LOCUS       DAOSOB010000001        69791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_418_length_69791_cov_70.329070, whole genome shotgun sequence.
ACCESSION   DAOSOB010000001 DAOSOB010000000
VERSION     DAOSOB010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 69791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69791
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_418_length_69791_cov_70.329070"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1055)
                     /locus_tag="PLS88_00005"
     CDS             complement(135..1055)
                     /locus_tag="PLS88_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOU05775.1"
                     /translation="MALSRIFSIFLLIAVGYVTRKTKLLEENGIRAVSNLVLTIALPC
                     TIISSFDRSIPFSAAKDLIEVAIYAIAIHGISIALSEILFSKMERTKQKILIYATVFS
                     NCGFMGFPLAESIFGPSSLMYVSIYVMIFNIFTWSYGVALLSRGENSDQKTKKTKIRD
                     MLLNPGIIAVAIGFILWILPFSMPRTISYAIALIANCTTPLSMIVVGATLASLGVKGL
                     FSGMEVWIGSFMRLLVLPAIFIIFMKILKQDDFPTKVANLLVAMPAAAQTVIFAERHD
                     ADVLLASKIVFISTVLSAATIPIIAQLLKV"
     gene            complement(1070..1849)
                     /locus_tag="PLS88_00010"
     CDS             complement(1070..1849)
                     /locus_tag="PLS88_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOU05776.1"
                     /translation="MRIIRCIYKDAIYYGRIEDDLVVLSKDLPQIRIHDKKKRIDFDR
                     EEAKVPFSSVYLEAPCVPTKAVCVGLNYRDHAEEFGLKIPSSPVLFIKPSTTLLGPGK
                     PIHYPRLSKQVDYEAELVVVIGKTAKNIDAKYALDYVLGYTCGNDVTARDLQPKDGQW
                     TVAKSFDTFMPLGPWIETDLDPSSLKLKAYLNNEPKQVSSTSNLIFDVPSLIEYISQI
                     MTLNPGDVIMTGTPSGVGPMQKGDSICIEIEGIGRLENTIE"
     gene            complement(1935..2840)
                     /locus_tag="PLS88_00015"
     CDS             complement(1935..2840)
                     /locus_tag="PLS88_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOU05777.1"
                     /translation="MNKYLDISEEVADAITTGIPVVALESTIITHGMPWPQNLETALK
                     VENAVREEGAIPATIAIIKGRLKVGLTREELVSLANGSLKPIKASRRDFPALMAFGGN
                     GATTVAGTMIIAEMAAIHVFATGGIGGVHRGAEKSWDVSADLEELARTDVAVVCAGAK
                     AILDIPKTMEYLETKGVPVIGYGTSELPAFYTQHSGVELSLRVDTPREIAMILRAKWE
                     SGLKGGVVIANPIPSQYAMSETTIEGAIQKAIEESEHKGLKGAEVTPYLLSRVADLTG
                     GDSLDSNIALVLNNARLAAKIAKEY"
     gene            complement(2837..3961)
                     /locus_tag="PLS88_00020"
     CDS             complement(2837..3961)
                     /locus_tag="PLS88_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOU05778.1"
                     /translation="MKIDRHRKPYCVVVGGLNLDIQAFCSFDYRPEDSNPGQIHRSLG
                     GVGRNIAENLVRLGLDVELITVVGDSSSWDGLVSRTEKLGISLAHSLRIKEAPLSVYL
                     CILDRDGNLMGAVADMRAIEKMQVNQLKAQKSLFDYAEAIVVDGNIPKDCIEWIAENY
                     GVLEDEKKPHISSIKQKSQEPDAISRPLLIADPVSTKKSEKFVSCFGKFDIAKPNVSE
                     SARIAGLEENASIEEIALSLRKKSLLPKELYISKGTEGISFIDREIIEHIPLNSFKLC
                     PHVINRSGAGDAACAALVWISLLERRLKEKYGDSSWSISPRDKAKLALSSALYASSSN
                     RPVNPHLNQRNLFETTRICYPETSKLIGELINAIQTENHK"
     gene            4035..4514
                     /locus_tag="PLS88_00025"
     CDS             4035..4514
                     /locus_tag="PLS88_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOU05779.1"
                     /translation="MDQIFDRLEMLVRAWMSSTFGEGLGRTRFRDSETFEDPDLADAW
                     DELESYLDPFKADKKHKARTNWRSYTYRSYDTGETLEEETKRKAVEEAYRFLGLEPYA
                     PFAQVKVRYKKLLKEHHPDRHTKSATDNAEANEISAKINAAYQLIEAWEESQAKSFR"
     gene            4532..7432
                     /gene="polA"
                     /locus_tag="PLS88_00030"
     CDS             4532..7432
                     /gene="polA"
                     /locus_tag="PLS88_00030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOU05780.1"
                     /translation="MENKAPLYILDVYAIIYRSYFAFIRKPLRNSKGENVSALFGFFR
                     FLFSLFEQKKPELFAAALDSIGPTFRHTQFATYKATRQKTPEDLTAQIPKIEAILQAL
                     EVPAIRSEGYEADDIIATLANQCRKEGRPCFIVSGDKDLLQLVGDKIFALRPSENFSF
                     RELDAQGVFNEWGVRPDQIRDFLALTGDASDNVPGVKGIGEKTAQKLISKFGSLEAIY
                     NNLTAIEPESLRKKLEEGRESAFLSKELISLCYDVPACPNDVSLLKPSLNIEAAAPLF
                     LREDIKSLIPDELRRQLLEKSTTRKEVASAASFQEISGRAQQVQARSSTQVMPSTQAV
                     HSSQASLSSEAAAKGITQGAASMVPIENTSGLSPSQLMQANYEAVLSIEKLDRIIDEA
                     IERAVCAFDTETDSLDVLKAGLVGFSLAFDADSAWYVPLKSPDSECIPKDLALSSLSR
                     LMKEPQMLLIGHNLKFDINVLYTAGLEVNPTCFDTMIAAWVLDVEAPSFSLSSVAKRF
                     LGIYGLEYEDVVPKGLTFADVPLASATRYASEDAYFSFLLYQFLSKEIETHNLHNIFY
                     DIEMPLLPILAEMERTGIRVEAGRLRAYGKELQKELTEIETKVFQLVGHEFNLASPKQ
                     LAEVLFSERKLPVQKRTKTGFSTDTSVLEELAPLDPVPQLILRQRLLTKLKTTYIEPL
                     AELATAQGRIHTTFVQTGAATGRLSSRDPNLQNIPIREEEGRKIREAFIAEPGHLLVS
                     ADYSQIELAVLAHLSKDPNLIKAFKEGVDIHKRTASFIFGIPEDQVDADRRRIAKTIN
                     FGVIYGMSAFRLARDLGISNSLAKEFIDSYFSTYSGVADFIQKTIKEAQEKGVTFTLF
                     GRRRQILGINSKNKTEQQAAQRVAVNTPIQGTAADIVKIAMIKVHRRLRKEMPEVHML
                     LQVHDELVFEAPQALVEGAMKLIKEEMEHAVLLDIPLRVSMESAPSWGEMHL"
     gene            7450..8070
                     /gene="coaE"
                     /locus_tag="PLS88_00035"
     CDS             7450..8070
                     /gene="coaE"
                     /locus_tag="PLS88_00035"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOU05781.1"
                     /translation="MFILGLTGGYCVGKNEVASILEKSGWKVTDVDKLGHVALPLVCN
                     ELIDAFGDSILREDGSIDRKKLGSLVFNNRERLTSLENIVHPAMLSLLDKEIEKAQQN
                     SIQKLCINAALLYRFPQLDSCDAVIEVRAPLYLRIDRAIQRDHLSFDEALHRISSQKE
                     LWRKRSDVSVPIYMLWNLKSLEELEADALELLKKIENEIKGKRSFT"
     gene            8111..8860
                     /locus_tag="PLS88_00040"
     CDS             8111..8860
                     /locus_tag="PLS88_00040"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU05782.1"
                     /translation="MSDRSKKLLWIAVAVSGFALIVLLAVFIIFPPNQLSESKPFDLT
                     GRSPAKEVSPNDFSAFPSIVIQSSEGEGQASSTSKDEKTEPQKPQSNVIIVPAPSSNQ
                     KTAETPSQPIESQSQKTAVAPKTSQTSQTSQTPAQSSAKTSPATTPSASSTQKTTQPT
                     PSAAPKTDSSSTEYWIQVGSFSQQDTAEKLRSEYITRGMTAIIAQKDVGGKTYYQVKV
                     GPYASSDEAKKWLLTVKSVKGASQDAFVTSR"
     gene            9005..10078
                     /locus_tag="PLS88_00045"
     CDS             9005..10078
                     /locus_tag="PLS88_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU05783.1"
                     /translation="MRKIILGLLALLMAVYLVLPATAQAKKPLKVAFVYIGPPGDLGW
                     TYEHERGRLALQKYFGDKIETKYVENVPEGPDAERIIRQFAIQGFDVIFTTSFGYMDP
                     TYAVAQEFPNIVFEHCSGYKTAPNMATYFGRIEEARYLTGIIAGRMTKTNKIGYVAAF
                     GIPEVVRGLNGFALGVRSVNPNAKIQVVWTNTWYDPVKEREAAIALLDAGCDIIAQHQ
                     DTTEPQKAAQERGKLSIGYDSDMAKFVGDTVLASAIWNWETYYIKTIQEVLNGTWKTH
                     AYWGGLKDDIVKLSDLSPRVPANVRKEVEDAKKKILGGWSIFTGPIKDQNGKIVYKAG
                     EVIPDDKQLSMDWFVEGVVGKVQ"
     gene            10142..11668
                     /locus_tag="PLS88_00050"
     CDS             10142..11668
                     /locus_tag="PLS88_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU05784.1"
                     /translation="MNDSNIPKVEMRRISKSFPGVVANDCIDLKLHKGEVLALLGENG
                     AGKSTLMNILCGLYRPDQGEIYIDGQQVEIHSPRDAQKYGIGMVHQSFKLVDSMNVLE
                     NIILGLRNEPFVPDMKLVRKQLMELASQYHLSVDPDACIWQLSVGEQQRVEILKLLYR
                     NAEVLILDEPTAVLTPQEAHELNRVIKTMKQEGKSAIFITHKMEEVIEFSDRVMVLQK
                     GRVVAETPTSETSPKELARMMVGREVLFQIERKPYSPGKAILELSHIEALGDRGLPAL
                     KDLSFSLKAGEILGIAGVDGNGQRELAEVVTGLRKSTKGTLKIADKDMTNKSPLEIIR
                     EGVAHIPQDRASVGAVGDLSVASNLAMKQYRSKPLAQGIILLPKRIIDFARKLIDKFR
                     IATPSPNTQVKFLSGGNVQKTILAREIGAARSLIIAVYPSRGLDVGATESVRRQLINM
                     RDEGLGVLLFSEDLDELMQVSDRIAVIFEGRIMDIFDAENIDIEHIGMLMAGMKTEVY
                     "
     gene            11668..12747
                     /locus_tag="PLS88_00055"
     CDS             11668..12747
                     /locus_tag="PLS88_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU05785.1"
                     /translation="MRFVFEKRKATSISALILVPLISFAVSLCLTALLLTIFGADPLK
                     TYSAMAIGAFGSLHGFAETLVKAIPLMMTGLGVAIAFQLKFWNIGAEGQLTLGGVAAA
                     GVALFLEQYLPGPFLLVGALLGGLLAGALWAGIPAILKTSLNVDETLVTLMMNYIAIL
                     FSEYLYYGPWRDPKGYGFPGSRMFSSNAWLPRIVGRAHIGILIALFVALVLWFILRRT
                     RWGFELQIIGASQQAARYQGVAVERNIVLAITLSGALCGLAGAFEVTGISHRLQQGLS
                     IGYGYTAIIVAWMSQLNPLVIPFVAVALAALSVGGDQVQMIMGLPAAMGTVMQGLILF
                     PMLAGSLFTEYRLLAVKDNKSNLPG"
     gene            12764..13714
                     /locus_tag="PLS88_00060"
     CDS             12764..13714
                     /locus_tag="PLS88_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU05786.1"
                     /translation="MDFVTSILTITIRAGTSLTIATIGEIFTERTGILNLGVEGIMLM
                     GALSAFTAVFYSHSIMLGILVAMVIGGLLALIHAFLTTTMRANQVVSGLSITLFGTGF
                     SSFLGQRLGPESNNFRLVGLRAERILPFAPKWAKDIPILSAFLNQDIITYLVYLLIPL
                     AWFFLYKTRPGLWLRSVGEDPYTADAMGIDVTKTRYFYTIIGGMLIALGGAHLSLSYT
                     PGWSENITGGRGWIVIALVIFSMWNPARAVWGSLLFGGINAVQFRLQASGTSIPANFL
                     NMLPYIGTIIVLVAITWWEALSKKVGAPAALGSSYMREEK"
     gene            13711..15447
                     /gene="ade"
                     /locus_tag="PLS88_00065"
     CDS             13711..15447
                     /gene="ade"
                     /locus_tag="PLS88_00065"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOU05787.1"
                     /translation="MKKDIIDAAIGKVQCDVNYSNCILVDVLSGRLLKDSVVSIKNGY
                     VAGINDGLSAKETIDLRGMYLAPGLVDGHVHIESSLLTPAEYAKVVIPHGTTTVIADP
                     HEIANVMGYDGMRYMLNVSENIPLDVYFMVPSCVPATDFDTAGAALYASDMHQFLQEP
                     RVLGLGEVMNYPGVLAQDPQLMDKIALFKSNGRPIDGHSPGLSGARLSAYIAAGIGSD
                     HECTSAEEALEKVGKGMYIMLREGSTAKDLRNLLSAVRRENAGRFMLCSDDRHCNDLR
                     DEGHIDFSLRLMLEAGVDPIDAIRIASSNAARWFGIPSQGAIAPGYKADFIVFPSFES
                     FEIKAVVKNGKIVAKDGSLIEDFSGAPIAIRDSVNIKWLTAEDFIIPDKGSPVRVIKV
                     QQNSLITIHGIEELPFENGALVSDLERDILKIFVIERHTGSGNIGKGFIQGLGLKRGA
                     IGSTISHDSHHMIIAGVDDLSIFKAARHLNKIKGGLVYAVGDQILLDLPLPVAGLMSD
                     KPADFVIKKLSDFDRLFQKEGLTATSPLMTLSFMALPVIPSLKITDKGLVDVEKFKKV
                     SLYADKIVKEKH"
     gene            15612..17138
                     /locus_tag="PLS88_00070"
     CDS             15612..17138
                     /locus_tag="PLS88_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOU05788.1"
                     /translation="MAYLRIKVNNTWHDIHSSEGTPAIDVIRGELGLMATKEGCREGD
                     CGACAVLIGEYAASQLRYRALPSCLLAIGELKGKHLITLEGLIEGAQDGLTPVMRAFL
                     DENASQCGFCTPGFIIALTSWLAEPKPLDLAGALQAIDGNLCRCTGYAAIRRAALRLI
                     EQFKGLPIDPEARLNALVGVHVLPLSVSEFAAEISRQFSQNSIEKKNPQIAPKERITS
                     NGIVILGGGTDYYVKNPEPGEDFSPFLIRMRPEFKEIAYLEEKGQKWLEIGAAVTVRD
                     FFASELVRQEVPGIEQFETMFASTLIRNLATIGGNIVNASPVADITAMLLALDAELVI
                     SSQEEEGFGTSITARTCKLEEFFLGYKTIDLKPTEVIRAIRIPCCVDPKTRKFSFEKA
                     SKRKNLDIAAVNTAISFRIEEQRFKDVHISLGGVAPIPIVSKAAMELLEGSFCPISDG
                     KTMAELAKKTASLAEASVKPISDVRGSEEYRRRMVYRLMLAHFVRLFEDSGVAEELFP
                     "
     gene            17135..19543
                     /locus_tag="PLS88_00075"
     CDS             17135..19543
                     /locus_tag="PLS88_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU05789.1"
                     /translation="MNNKDILEQDEFRAREANSDMSLKWAGRAELESGSSGLIPESLP
                     NVLGQTQYIDDIPRPAGCLQAVVVLSQSAHGKIHSIHIEKAIELDASVRVILAKDIPG
                     TNQIGFNKPDEQLLPEEEWHYWGQPLAIVVATSRSLARKAASLVRIEGENLPAIIDAR
                     EAATKGDFIFPPRTIACGDVLEAFSKCAFVVEGRVDSGGQEHVYLETQGAIAQVIDGR
                     RVHVISSTQGPTGVQRAVAQALGLPMNMVEVEARRLGGAFGGKEDQGANWACMAAVAS
                     WVTGKPVEIYLNRKDDMRATGKRHPYSTDFRLGADANGKLMAFEADYYQNSGSTCDLS
                     AAILARTVLHATGAYQIPNVRVTGYMCHTNLPSFTAFRGFGGPQAFFVIESAIDALAQ
                     KMGIDPVEIKRNNLFQEGDVTYYGMTMERVRASDSVEQLLRKVDYPALKARIAEFNAS
                     HRLKKKGLGLIPVAFGISFTKLQMNQAGALVHIYSDGSVVVSTGAIEMGQQVARKIAI
                     IVAKTLGVPIDRVTVQRTTTLTVANTVPTAASTGSDMNGMAAKIACQEIRGRLLKKAA
                     ELLMVDLEKIDIKDGAVLVDGAPDHLSWEQLVEAMSASRQDLSAHGFYATPMLEYDME
                     TERGKPFAYHVYGAALVEASIDVLRGTYTIDRATIVHDIGESIDESVDIGQIEGALAQ
                     GLGWALLEDLRFEPDGKPISDTLSTYKVPDINFMPPNIDIEFLPSIDNPFAPYNSKAV
                     GEPPLQYGIAGYFAVLEAIRAATGKTLEYYDLPLIPEKIMKLLISEQNKIDKVTGNHI
                     GTKA"
     gene            19574..21055
                     /locus_tag="PLS88_00080"
     CDS             19574..21055
                     /locus_tag="PLS88_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU05790.1"
                     /translation="MILANVAAALPGERDFRLVNIFIKGNKIASIKEADLKKKTDNPL
                     ENINSGANEATDLHAYDDEEIIDAHGLLAFPGAIDPHVHFDEPGFTHREDFLHGSAEA
                     ARGGVTTIIDMPCTSIPPVISNEALEEKLSIVQKKALIDFGFYGGINGNMTPEHIPEL
                     IASLAPRVLGFKSYFISGMDTFPAVDEAQFAAAAKACGKEGRPLLLHAEDAYVISEAT
                     KERELLRGAQKPQWIDYYASRPMEAEIVAVMKALKLAGQYAKYVHIVHIGTAKAAMAA
                     FERGATCETCPHYLAFDETDFARFGAALKTAPPVKSPEQKSILWHLLADGKINFVTSD
                     HAGAPDYEKFTDNPLMAYGGIPGTGTLFPYMLSEGLFAKRLSLERFLEVTSGGAARRY
                     GLWQGKGSLAPGKDADIVLVDPEHTRRLNPSAMMSKSAITPFAGMVLSGRIEGTWVRG
                     SIVYASVRLAAALGAPKDPIFSNEEGIILAKPGYGTFLTWGYR"
     gene            21052..24267
                     /locus_tag="PLS88_00085"
     CDS             21052..24267
                     /locus_tag="PLS88_00085"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU05791.1"
                     /translation="MSKIMKATALPALFARIAGEYKAKRSIFEIPESTWLDIFEQEAE
                     SAGMPVMASTISIPLGPAAGPHTQIAPNLIAAYLSGARVFELKTVQENDHLDIDKPCI
                     DALDEGYNVEWSTELSLEEARIEYINAWMVINLFARLWSHKPSDFLFNISVGYTLEGL
                     KSEKMDAFIEGMRRPETGSYWEPALEELKSFVESPLFIQAFGSQALEKARSIADHMPV
                     RPVHSVTLSTMHGCPPEEIERIGRYLIEEKGFDTYIKLNPTLLGYENARTILDRLGWK
                     DIVLKRESFEHDLQFDMAISLINTLGEVAANRGRRFGIKLSNTLANINNGDYLPGMER
                     YLSGRALFPITIHLTARLAHALPDFGARFSYCGGVSAFNAADLIRAGIGPLTIATDLL
                     KPGGYQRLGHMVKDVLSSLPFAPESPDSAALDALAEGVFLKPYYRKEWKEGMASIEGP
                     LPLFDCFAAPCIEACPVVQKVPAYVAATGTGDANRGLSIVISDNPLPTITGILCDHIC
                     QEHCSRVDYEGAVRIRDMKLSTVRAANSESENEMRPAWPREAIGKTAIVGAGPAGLAC
                     AWYLAQHGQKAVIFEKLPIAGGVPSTVIPSFRISQEDIASDIDRFEKLGVEFRFGTEI
                     STIEACKALERDEFDSIVIAHGAYHARNLMLGGDGVKVLHALDFLRSCMEKGLDPFSG
                     AKNILVVGGGNTACDAVRLASRIPGVEHVYWSYRRSKNEMPADKEELANAIAEATALM
                     ISPDNRASYFSQDPSSSPDSSSEEKNQESIFLELTLPEALGPGTITLRRMKLGEKDES
                     GRPSPVPTDETFSLPCDLMIAAVGEAPDPTLFKNFGIEVAKNGLPIVDEETMESSIPG
                     IYICGDARRGPSSIIASEADGRAAALAILKKREIEVINKDYRAPLWDSTSRLNRGKMM
                     ESLSIDDPSFAAIEAERCLACDSACLRCVEVCPNRANIIIETGRLFDQPAQILHIDRL
                     CNECGNCGRFCLWKGEPYRDKPTLFDTIGDLHVSNNAGFAFSGNRERPSLVFRAKAGE
                     SVAELPFFAWNGVISLSVHRDILALARVVWSEHRYLVEVHE"
     gene            24264..25622
                     /gene="ssnA"
                     /locus_tag="PLS88_00090"
     CDS             24264..25622
                     /gene="ssnA"
                     /locus_tag="PLS88_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOU05792.1"
                     /translation="MILFRNVRIMELEPPSVSEPIDVAVYEPDEGAHGGTIAEIGTSL
                     QTKYPKAKLAGDGGFLSPGLVCGHTHLYSVLSRGMLVDIAPSKDFAQLLEHLWWRLDR
                     AIDPEILQASALAGCSDALKAGVTSLVDHHAGPECIADSLSIIRKAYEDTGLRGILCY
                     ETTDRNGMAEARAGIAENVRFAKEVDNIRASGLKPLVEAAIGAHASFTLSNETLEGLA
                     EAVQETCRGIHIHLAEDKFDAVDARYRFGKDPVERIDAVGALNPRGLIGHGVWLTPSE
                     VEIMNARDAFLAHNARSNMNNAVGYNTLLPTFKNVVLGTDGMGADMLEEFKFAVFRHR
                     ESQGPWWPGDFLKALHRGNLLIERYFSQDFEPTFPGFGTIKRGAPADLVLWDYDPPTP
                     LVGANIAGHFAFGMSSRSARTVMVAGSIRILDHRPLYDDIAIEAEACHQASRLWKRME
                     ER"
     gene            25626..26846
                     /locus_tag="PLS88_00095"
     CDS             25626..26846
                     /locus_tag="PLS88_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOU05793.1"
                     /translation="METTKILEKTHDYRDYTAQNLSKIIKVPAFSTTEKERIQLLKKL
                     CEEAGMEDLWIDGLGSLLGRVGKGSKKLVFDAHIDTVGVGDESQWKTPPFSGLIKNGL
                     VYGRGASDQLGGAASMITAAHILKELSYEGDYEIWFSFTTIEEDCDGMCWKYLIEEEK
                     FVPDFVVSTEPTSCRLYRGHRGRMEIQIDIKGISCHGSAPERGDSAAYKAARAALAIE
                     KLNTQLLPDDDRFLGKGTITVSQIDVHGPSQCAVPDQAMLYCDRRLTWGETDKSAIAQ
                     VQAVLKEAGVDDFTVYMPEYRKPAWTGTEYHQELYFPTWKIPEDHILVQSGVEAYTAL
                     FGEKPIVDKWTFSTNAVAICGRHNIPVIGFGPGDEAQAHAPNEITRVDDLEIAAAFYV
                     ALPFALSALPTRER"
     gene            26867..28093
                     /locus_tag="PLS88_00100"
     CDS             26867..28093
                     /locus_tag="PLS88_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU05794.1"
                     /translation="MKWFYRCPLCGKTYPIEPGRYLCDSCAEAQKPNEPLRGILECSW
                     EGENSSRGSIPLPVEESFFPPIPVGQTPLWAPERLRTELGIPHLYLKDDTCNPSGSYK
                     DRASWLVAAFARKFGIKEIVLASTGNAASSMACIGAASGIAIKVYVPKSAPIAKRVQI
                     LQYGAELIEVDGTYDLAFDKSLEYSKSTGMLSRNTAYNPLTIEGKKTASFEIAKDLAR
                     AEDNVKKHGINTNVSQDEALKVPNIPAYLAPDHIFVPTGDGVILSGVIRGFEDLLRLG
                     WIDRIPTVWAAQAEGSCAIARALATGVFEARPSRTIADSIAVDLPRNGYFTLHKLKKH
                     GGRAVVVSDEEILKAQTYVARLSGLFAEPSSACAFAGFMKVRSELDPEARVVVMLTGN
                     GLKDIKSAAMAVGLQT"
     gene            complement(28146..29522)
                     /locus_tag="PLS88_00105"
     CDS             complement(28146..29522)
                     /locus_tag="PLS88_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOU05795.1"
                     /translation="MDKPLRVLLIEDSEDDAVLILRELRKAGYTPLSERVETEYTMRS
                     ALSSQKWDIILSDYNMPEFCAAEALSVYRECGIEIPFIIVSGAVGEEKAVQLMKEGAH
                     DYVMKDNLKRLVPAIERELKESEDRKSRKIAEDSYRKSDFIVNASRDMMALINRSGIF
                     ETVNSSFCKIFGKERQQEIVGHSVSEIWGQEVYEQKIKPCLERCQNGEDINTEDWFDM
                     EGMGTQCFEINYIPYRNSDGVVTHAIMAAHNITNRKMAEQELDKSYRNLQKTLEETVN
                     ALSALVEMRDPYTAGHQNRVARIAKAIAQQLGLSEDAAQGIWVASLIHDIGKVRVPAD
                     ILSRPAHLSSAEFELIKEHPQTGYEILRKIDFPWPIAEIVLQHHERINGSGYPLGLKG
                     DEILFASRIIGVADVVEAMTYHRPYREALGLDAALDEIKENKGILYDSDVVDACIKVF
                     LEKGLALD"
     gene            complement(29567..31735)
                     /locus_tag="PLS88_00110"
     CDS             complement(29567..31735)
                     /locus_tag="PLS88_00110"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU05796.1"
                     /translation="MLSQFLNSANYFLNPYSIPMFITSLLVLLLGLYVLLQDKNIVSQ
                     SFFVTTASASIWQCGTGLIYSMKNSALVLSFCKVFTFLGIIMIAPGIYFFTITSLDLY
                     NEKKRYILANYAIALVFYIFGLLTDWLIASVEERFYGYDIVFGPLVIPFLLFFSVLII
                     RSLQLDRRAIRQMEPGAKRDQIRLFMISLSIAALASVDFLSSIPSLHFYPIGFLLVLV
                     FISLQAYAIIKYKRASLGTIFEAIEDGIIVIDKNGKIEEANPSMETMLGIKRHDLLGK
                     DFLSIVSLVSSGTEDPEKVEAMIKNLLLTPAKIADEDIAFKDSERHFNVASSPILDRF
                     GAKTGTILIFRDITERKKMEEELRRYQGSLEEMVKARTKELKESEEKYKALVDHALVG
                     IGIHQNAQMVFANRQFASMLRFSEEEMIGLPILQIIHPDERELIMSRAYNRYSGKEVI
                     ETYEMRMVRKDGSFMPAFISNTAIEYNGERATLITVVDMTETKLRKELEQVNQELERF
                     TYSVSHDLRAPLRSIDGFSQALQEDYEDRLDREAQDYLHRIRAACQRMTAMIDAILQL
                     SRIGRYEMHREKVKLSALAQDIALELETTYPDRKVKFSITEGIVAAGDPMLLRMALEN
                     LIGNAWKFTQEREGARIEFGVMQKNSKTIYYVRDNGTGFDMKYADKIFDPFQRLDNAM
                     AFSGTGIGLASVKRIIHRHGGEIWAEGEVDKGATFYFTLQ"
     gene            31980..33479
                     /locus_tag="PLS88_00115"
     CDS             31980..33479
                     /locus_tag="PLS88_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU05797.1"
                     /translation="MIDLAIDESQRKKNIEHCKKRGILLPTFAQMCDPAKIPSAIRAE
                     LYGIGLWDVHPRNLFRVNWHNEPKEFGGGYGSVNFIEIPRSISGTKARIIGLAGKWFP
                     TGAHKVGAAYACLAPELVTGRFDPTSKKAVWPSTGNYCRGGAYISRLLACPSVAILPE
                     GMSRERFDWLKTMAEEIIATPGSESNVKEIFDKCVELQKTRQDVVIFNQFDQLPNHLW
                     HYAITGPAMEEVFATISGAATQAGEKGTHANGESAQVCGVVLSSGSAGTLGSGSYIKE
                     KFPGAKLAVAEALQCPTILENGFGAHRIEGIGDKHIPWIHNLRDTDAAVGVDDELPMR
                     LIRLFNEPAGRELLLETGTDPSIVEKLDWLGISGAGNLIAAIKFAKYYELGEDDIVFT
                     MFTDSMELYQSRLVELTAERGPYDQRQADRDYECLQDLSVDYVFEMTHVDKRRVHNLK
                     YFTWIEQLGKELPELRAQWSDYRNYWGSLHQQVAALDELIEDFDAEVQK"
     gene            33476..34837
                     /locus_tag="PLS88_00120"
     CDS             33476..34837
                     /locus_tag="PLS88_00120"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOU05798.1"
                     /translation="MILLKDCLRVVRLEDEDLNGVDVLLEGNRITQIAKNISIPCGAR
                     VIDASHLVVAPGLVNTHHHFYQTLTRNLPAVQTAKLFDWLVYLYEIWKGIDEEAVYIS
                     SLLAMAELIKTGCTTTTDHHYVYPQGFEADIPTLQFKAASDIGIRFLATRGSMSRSKK
                     DGGLPPDSVVQDEESILAHCEDTIRKFHDPSADSMHRVALAPCSPFSVSEELMKESAI
                     LARKYGVRLHTHLAETADEEDYCVQIYGRRPLRLMEDCGFLGADVWYAHGIFFNDKEI
                     ELLAKTRTGIAHCPSSNMRLGSGICRVREMLDIGVPVGLAVDGSASNDSSDMLGEARQ
                     ALLLQRVRYGATGLASKEAWRIATKGGATILGWEQAGSIEEGALADIACWNVDKLAYA
                     GSLSDPSAALLFCGYDHAAEMVIVNGKIVVDNGRIVCIDEEKLKEDANKVALRLYKKA
                     GIT"
     gene            34855..36276
                     /locus_tag="PLS88_00125"
     CDS             34855..36276
                     /locus_tag="PLS88_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOU05799.1"
                     /translation="MNTTALIIVLVYLAAMLAIGFWTNKKLIKTSADYMLAGRAVPMI
                     IVACSLAANNIGGGSTNGLVNRAFGSWGISAVWYVMAASIGLIPMIFFAPKLRGVMAF
                     TIPEVVGRRFGKTAHITSAILNIVSLFCLTASQILTSGTILSLLIGLDLNFAIVLAAG
                     FTILYTTMGGLWADSITDLFQWIIIFLGLLIALPFAVNAAGGWNNMMSSLPSEKLKPF
                     GTLGFLGILSLVINYFLTFTAGPEMVSRIFSAKDAKASKNALIWAAVFMGVFSFVPTF
                     IGLAGYAINPNMPSNQVLANVIFGHTPAWVAGAVSAAIIASTMSSADSDMLCASTIIT
                     KDLLPYFKSDLSDKSQIAISRAMNVIIGIGAMFIALFKIDIITLNVFSFMLRAAGPIA
                     AFLLGLLWKNVGKAAGVISIFVGSAVGIGWQILANQMKVDNPYGFLPIVVGSASSFIT
                     FIIVTYIERAMGRPPAPELKIEE"
     gene            36359..37576
                     /gene="ygeW"
                     /locus_tag="PLS88_00130"
     CDS             36359..37576
                     /gene="ygeW"
                     /locus_tag="PLS88_00130"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOU05800.1"
                     /translation="MDRNQIENLIDDLRGKKIAMCKKDFLLTWEKTVPEIEATFLVAD
                     ILRAMRDSNISPKVWDSGIAVSNFRDNSTRTRFSFASACDLLGLYIQDLDEGKSQVAH
                     GETVRETANMISFLTEAIGIRDDMYLGEGDRYQREVGKALDEGVKAGVLPSRPTIVNL
                     QSDIDHPTQAMADFLHLVHYFGGLDKLKGKKIAMSWAYSPSYGKPLSVPQGIIGLASR
                     FGMNIVLAYPKGYDLIGDVVEMSKKQAAESGGSFSYTDSMDKAFKDADIVYPKSWAPY
                     DVMLRRVPLLHAGDKEGLKELEREALANNAKFKDWECTEEKMKLTRSAGKNSPQALYM
                     HCLPADITGVSCAAGEVAASVFDRYRDDTYREASYKPYIIAAMILLGKFERPAELLES
                     LLARGRQRTYGNF"
     gene            complement(37634..38395)
                     /locus_tag="PLS88_00135"
     CDS             complement(37634..38395)
                     /locus_tag="PLS88_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU05801.1"
                     /translation="MNSESVLSARDISFRWKEDQPLLEDISLDVARGQTLAILGPNGA
                     GKTTLLSILSGRLSPRAGSVNLDSRPLISYSARERSRLIAFLPQLEKLAFNYRVLDFV
                     LMGRTPCMEVLALPGKEDEEAALKALSLFGLDSFSMRNIGELSGGEFQLVRIARCLAQ
                     GASILILDEPASMLDPAHSRHIADALASLADSGKTIIYTTHDIDLAIFLGGHALIISE
                     GKIQWIGAAGGLVNEDILGRAYGISFSIKQLPSAF"
     gene            complement(38392..39408)
                     /locus_tag="PLS88_00140"
     CDS             complement(38392..39408)
                     /locus_tag="PLS88_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU05802.1"
                     /translation="MKQGRSSPNSPHHSSLVIYLGIALIIVTVLSLFIGRYPVPGIMN
                     PCLLKTDPIARQIVLNSRLPRILGAILLGLVLGGAGASLQSIFSNPLIDAGFLGVSQG
                     AAFGAALALVLGFRAYSVIAALAFGMAVLALALSIWLSERFRYGGQILRLVLAGLAVS
                     AFFSSLLSLMKYIADPLHQLPDIVFWTMGSMVPMSWKRLESAAPIAVLSLIVLYAMRW
                     RVNLLSLDDAVAHSLGVRPSLERRIVSVAAAAGVGVMTAVCGVIGWVGLIIPQIVRAV
                     DGPDGRFVVPRSMLGGAIFVLISDTIARSVFSGEIPLGIITSLLGALSFAILLTTRKV
                     ELAR"
     gene            complement(39410..40426)
                     /locus_tag="PLS88_00145"
     CDS             complement(39410..40426)
                     /locus_tag="PLS88_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU05803.1"
                     /translation="MKRQIILFGLVLAIVMAFSLNAQQRIVLAGRGANMLADAIYLFP
                     DAGERLVAYSSGDQGLGLFMSAVDPAFKEKVCFDKSAGVEVIASFKPDLVILKSIAKS
                     QYEAPLNALGIRQLYLTLETPEQYYEDIATLGKVLGNQVQAAEILSWYADHEKRIVSH
                     TSKLSASQKPKVLLMQLAASGESVWQVPPDSWMQTIIAERAGGIPVWKGANPGSGWAT
                     VSVEQIAAWNPDVVCIINYRGNSSEAAEAFKKDKRLSSLKAVKEGKVYGFPQDFYSWD
                     QPDTRWILGLTWLAKQIQPALFRDISIVQTTRDFYSFMYGFDEAKFRSIIEPKIKGDI
                     GEQF"
     gene            complement(40496..42076)
                     /locus_tag="PLS88_00150"
     CDS             complement(40496..42076)
                     /locus_tag="PLS88_00150"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOU05804.1"
                     /translation="MDRESENYQGYDTILNADDIKIFYPANSRIAGSQTTFIVSEVRG
                     ASAYQIQISSSQSDFENNILLKNSDYASNKIDIGLQSLTRGIWYYWRARAQQSGEWGK
                     WTEISSFIIGYTITYNGNGNTEGSVPIDSNIYLEGESAIVAKRSGSLIKAGYGFDSWN
                     TRSDGTGINLRAGSTLVVSQENITLYAIWHLLSLWAIGCNNYGQLCDGTTTNRYIPIE
                     VMEDVKAVAAGEEHTMILKNDGSLWAVGRNSEGQLGDGETNRRPTPIKVMDDVQAVSV
                     GRYHTMILKNDGRLFATGDNFYSQLCDGTNITRYIPIEVMEDVKAVAAGWTQTMILKN
                     DGSSWAAGMGWDDMDYNGAGYGPMKPIKVMDDVKEIEPGQDSYSMILKNDGSLWGRGG
                     NYHGQLGDGTTHDRSSPVKVMDNVKAVAAGFEHTMILKNDSSLWATGSNEYGQLGDGT
                     THDRSSPVKVMDDVKAVAIGAYFTMILKNDSSLWATGSNEYGQLGDGTTTTRYLPIKI
                     IEDVEDIKAGYDYALILK"
     gene            complement(42197..44692)
                     /locus_tag="PLS88_00155"
     CDS             complement(42197..44692)
                     /locus_tag="PLS88_00155"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOU05805.1"
                     /translation="MRSRLNKIAICLAIFGLSFLFFGILPISAQNSANRYALVIGNAN
                     YSKLPKLRNSVNDASDMADLLKELDWNVTVLLDADLPKMEEAVLRLGNNVSTSPSAVG
                     LFYYAGHGVQANGVNYLIPADADISGETFLKTKSLSVQSVLDELQKARNNLNIIILDA
                     CRDNPFSWARSGSRGLSVLSYQPPGSIIVYATSAGSVAREGSGRNGIFTAELLKNLKV
                     PGLEIEEVLNRTCSAVQEVTYGEQNPAVYKQFFKSFYFNELKEKQKPIEPVILSSSQA
                     ADLLVRASEPDSEIYLNDVYKGKAPLLIQKVEPKKPLKVSAKTATSSGTDEVTLEPGE
                     LREITISMAPLRGNLVIITNESDVEVLIDGESRGSLGSGIFRSLPLGSHNVELLGKDS
                     YYSEKVEIRADTTTQMTATLTPVGSVDIRIPTDALAKLHIGSEIRIIEGSGIVRNLPE
                     GNYYIEASGKDYVSASAFLNVRKGQVVSWEPYKNGFISFDVLTKGSTLFIKELTRSYP
                     AEGQISDIPPGLYHGVLTKSGYKDSEIEIDVQPGKNTVVKVSLFMQEPARIVMKNYGV
                     DLAMYVNDKYWNATVEKDGLIYYDNLPINTPLKLEFELSSYKSGESISIPTRTLKLEQ
                     GQTLKLELDAGQIRIPLADGISYINVGGRSYPVFPSIDDEFITPWLPVGTYYLSINCA
                     PGTAYTGYVEVQKDSVTSFTDHIAQIITSLQAQRAETAKRLEKAKDTKINAQLSLGVG
                     IVGAIGAVASYIEGAKTKSAYNLATDSDEIASLRTQVQLYQVLFPVAATIGGAGLGTS
                     LVLFGQSNTSSLQKSIDDIDEQISELQRRGWNL"
     gene            complement(44810..46735)
                     /locus_tag="PLS88_00160"
     CDS             complement(44810..46735)
                     /locus_tag="PLS88_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU05806.1"
                     /translation="MSQKFYHLRWIREVWRERQALVWLLLFLTLLSSAVAVAFPLLTK
                     QLFDLLEHAIDTKMSEDAAMVQVRRIALYFAAIGVAGLIAGIFPGVRGALNMVFDYIV
                     RKRYFGEVVRKDIRFFSKFRSGDVITRLTSDISDFPKISWFLCSGIFRAVESASKVTF
                     CIIAMFSLDWRLALASLASLPVMLLVFSRTQAAIYDRVNKNEQAISEINNQLEMSFSG
                     VRVIKAFASEEKYTNFFKDALGKRFNTEMDVIKLETVLHLIYQYIDYVAQIALVFVGG
                     LMVTKRSISIGTFYAFYNYLNMLIYPVLDIPQLFVFGKRAFVNIDRLEEMRQYETGSQ
                     KQCSELSQKKQNPSLQNRNSVLSQKKNSPPLQDQNRVSLQKQQESIKKNDIANSDVFR
                     SLHFENVTVRYEQRRKAALSDISFEILPGEKFLIIGPVGSGKSTLIKTALGLLEPQKG
                     RVVINDKPVRSLDPTEIRGLIGYVPQDPLLFSGSIRENILFGCREGQILTEEELLRII
                     EVSRLTEEIEYFSDGLETKLGQRGTSVSGGQKQRIAIARALAGYPGLLLLDDMTASLD
                     TNNEEALWHSLAAMPEFARAAVIAVSHRLSSIQYVDRVLFLNMGRIAGFGKHAELMEQ
                     NAAYREFVAEHVRRPSK"
     gene            complement(46732..48489)
                     /locus_tag="PLS88_00165"
     CDS             complement(46732..48489)
                     /locus_tag="PLS88_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU05807.1"
                     /translation="MQKKLDSNDYLSTIMRYLSRYRMLALPGLIVMIAIALGQLAGPY
                     ILKQIIDVAVPREDTSLLLAYAAAFLVIVSVTGALSYLGMILLARLGLSIVTHIKQDI
                     FSHLLKLPVSYFDKHPVGELMSRTETDTERVRDMFSTLGANLIVNVLTMIGIFIVTFA
                     LMPALALIMVGISLGLLIILILFFSKIFPMYEKARSLYAKITAKVTEFIQGIEVLKAY
                     GRTGWAEADLDTTAKERMSLEVRISLIEYSMMSLLNALIGPMFIVVLLLLYAPKALTG
                     AMTLGTILLFVEYGARLLRPIAEIAESLRSLQQARTSLSRIRTIMATPEEPNRGSGKA
                     PTFEREIRFDHVWFAYEDEDWVLRDVSFVIPKGSFTAVVGASGSGKSTIISIICGFAF
                     PQRGKVLVDDIPLDEIDIVAWRRHIGLVLQESFMFPVSVLENVRLYQEEITEDKVKSA
                     LSIVHADKAVSMLPDGLEANLWERGSNLSSGERQLISFARALAADPDLILLDEATSNV
                     DMTTEKHIKESMDVLRKGRTMVVVAHRLSSILKADRIFYIDEGRLIAEGTHESLYEEL
                     PAYRLLVDQQFPAGKRVLV"
     gene            complement(48677..50491)
                     /locus_tag="PLS88_00170"
     CDS             complement(48677..50491)
                     /locus_tag="PLS88_00170"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOU05808.1"
                     /translation="MEQTKDFLLQPPKNRWKGSPPKIGIRPAIDGRRRGVRESLEEVT
                     MGMAKAAARLISENIRHPTGEPVECIIADTTIGGVAEAAACAEKFAREGVAVTLTVSP
                     CWCYGSETMDMDPLTIKGVWGFNGTDRPGAVYLAAVLAAHSQMGLPAFGIYGRDVQDI
                     SDAQRIPADVAEKILRFARAGIAVAWMRGKSYLSVGSVAMGIAGSIVNPDFFREYLGM
                     RNEYVDMSEIVRRIEEKIYSEAEFHRALQWVKTYCKEGPDNNPLGEQHSRARKDKVWE
                     TCVKMAMIVRDLMVGNPDLRRKGREEEALGHNAILAGFQGQRQWTDHFPNGDFLETIL
                     NSSFDWNGIREPYLVATENDSLNGVAMLFGHLLTGSAQIFSDVRTYWSPEAVERVTGW
                     KPTGLAENGFLHLINSGATCLDGTGCQRINGQPAIKPWWQVEPEEAKACLDATQWRYA
                     SLGYFRGGGYSSDFLTEGSMPVTMTRINIIKGLGPMIQIAEGHTVTLPPEVHNTLDLR
                     TDPTWPTTWFAPRLTGTGPFKDVYSVMANWGANHGASCYGHIGADLITLASILRIPVA
                     MHNVPAEKIFRPSYWNAFGMDPEGADYRACSALGPLYS"
     gene            complement(50517..51524)
                     /locus_tag="PLS88_00175"
     CDS             complement(50517..51524)
                     /locus_tag="PLS88_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU05809.1"
                     /translation="MARETVTIKNVAERANVSTATVSRVLNNDPRVRKETRNIVLDAM
                     NSLGYKLNFVARSLKTSRTHTIGVLSPDLSGDFFMFIAESMNRELTGRGYSLVVGTSQ
                     GSEEEEARQIRHLAERLVDGIVLIPVGGSSAYLPQISAFNIPIVFVDRILKGYKADKV
                     LVDNEEGAYKAVSALIREGHHRIGFLGGNPEVTTAEERYEGYCRAMEEAGLEIEQKYV
                     RFGNPTLPFGYRAMEEIMKDPYSPNTWFIVNVFTHLGATNYLISEGGERAKQITFAAF
                     DEMPYSPLLRYCRYAVQQPIAEMGKTAANLIIDRIEGKGPLEPQTLRFKTRLIQHTLV
                     R"
     gene            complement(51555..51992)
                     /gene="fucU"
                     /locus_tag="PLS88_00180"
     CDS             complement(51555..51992)
                     /gene="fucU"
                     /locus_tag="PLS88_00180"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HOU05810.1"
                     /translation="MLIGIKPCISAELLSVLYRMGHGDEIVLADAFFTGDSLNSRVVR
                     ADGIRIPDLLDGIFRLINLDSYVDDPIVMMAPSKGDSLDLEVEKRYREVLDRYWPETP
                     PIQRMERFAFYERAKKAFAVVMTGETVKYGNIILKKGVPPVTE"
     gene            complement(51986..52978)
                     /locus_tag="PLS88_00185"
     CDS             complement(51986..52978)
                     /locus_tag="PLS88_00185"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOU05811.1"
                     /translation="MIAVCGENLIDMVPSEHGKDLFQACPGGCPYNSAIAAARLGVHT
                     WFIGKTSKDFLGDKIISKLQSSGVDTSLLLRADQAMTLAFVERDPAGNAKYAFYSEGA
                     ADRCYYPEDLPPALPEACCFMLVGSISLVQEPSGSTICSLIEREYKKRLISFDPNVRP
                     SIIKSRAEYLERFDWICRRSAIVKASDSDLEWIYDMPPAKAAEKVLALGPELVAITMG
                     EQGAMILTKQHHLVSPAKKVQIVDTIGAGDSFHAALLAGLGWLKVRDRESLASLSEVN
                     LRAVLRLANSAAALDCQKRGAEPPTLRELAIFDPEAARIAPGIIPTFNSKEYIC"
     gene            complement(53125..54213)
                     /locus_tag="PLS88_00190"
     CDS             complement(53125..54213)
                     /locus_tag="PLS88_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU05812.1"
                     /translation="MHSAKRVRTVIVALVLLAALTVPVFAAGPIFIPMISKGFQHQFW
                     QTVMKGAKDAADKYHVVMTFEGPPTEADIQPQVQMLVNAMAKNPSAICLAALDTNSVM
                     DQLNEAIRRKIPIIGFDSGVPNAPKGAIYATAATNSYAAAGLAAQKMFPVIKDKIMAA
                     TPSNPITIIDFNQDATSQSVTDRGKGFRDMMIKLITTEGKRSLSDIKVTGNPAYIAPD
                     SPTSGKAIILEMVVPASPRDTDATNTAQAVLTRVQRDHIVGIFCSNEGTARAILSASN
                     DGTALPTQYPGLVVIGFDAGKAQKAAVRNRYFFGAITQDPYMIGYDAMELAYKAVMGQ
                     PVSDIDTGAKFYDYSNMDQPDIAQLLYD"
     gene            complement(54301..55581)
                     /locus_tag="PLS88_00195"
     CDS             complement(54301..55581)
                     /locus_tag="PLS88_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU05813.1"
                     /translation="MTSENVSVSRMQWFKERGLKYALAPAALIILYVFFGIFGMNYFS
                     YPTLVNIIDASYYIGFISMGVTFVIISGGIDLSVGTVMMASALIGGVALKHGVPMLPS
                     LLLIILVGTGFGFFNGLIVSRLKLPPFIATLGTMMISMGVGSIVSNVQSATFPIRGQP
                     GGWYKNLFLYLSPEGGMIPTGAILLLLVGIICHLILSNTKLGRYTYAIGSNIEAARLS
                     GVDVQKWQMMVYVISGATAGIGGISYAAIYTTVLPAQGQGFELYAIAAAVIGGTSLSG
                     GVGTIWGTIIGVFIMSVLATGLPAMNLQAHWQTFFTGVIVIGAVLLDMYRTKKASEVR
                     IETAADMYKAEMSEKIASMKRELSSLKASGDRDKVADLQKEIAAARRELKATFAAKKA
                     EEKAEQARIRAEERAADHEFSEMLKHKDHPIDNI"
     gene            complement(55578..57113)
                     /locus_tag="PLS88_00200"
     CDS             complement(55578..57113)
                     /locus_tag="PLS88_00200"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU05814.1"
                     /translation="MSVAAKLLSMEKIDKRFTGVHALRQVNFDLDEGEIHALVGENGA
                     GKSTLMNILVGIIQKDSGQIYYMGHLFNAHDPKHALEMGIGIIHQELNMMDHLTVAQN
                     IFIGREFTKMGGILLDKREQNKRAEELFTRLKMNIDPTEQVGRLTVAKQQMVEIAKAV
                     SHRLRILILDEPTAALTDTEIAELFTIMRDLKSQGVGMIHISHRLEEIHQIADRVTVL
                     RDGEVVDTKKTSEVTKAQIINMMVGRVIYEQPKIKSLVGPEAPVVLRAHKLNAGKLVR
                     DVSFELRKGEILGFSGLIGSGRTETARAIFGADEVQSGWIEVNGKRAKIRSPSDAVAK
                     GIGYLSEDRKRFELAINLSVKDNMVMAAYDQFQSSLFIDARKVQDVTQKYIDRLNIKT
                     PSIEQLLRNLSGGNQQKVVVAKWLICNCDILIFDEPTRGIDVGAKSEIYTLMNELTKE
                     GKSIIMISSELPEILRMSDRIIVMCEGRITGELPIEEASQEAIMSLATMGRQAVLTGS
                     GTE"
     gene            57393..58148
                     /locus_tag="PLS88_00205"
     CDS             57393..58148
                     /locus_tag="PLS88_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOU05815.1"
                     /translation="MLLDLSERERSILELLADRNYLSVSELSSTLGVSEVTIRTDLSS
                     LENRGYLMRTRGGALPSIHRSILEHQKLHIEEKQHIAKKAAELVCDGDRIMIEAGTTT
                     ALIARYLIGRQDVQIVTNSMLAFSYARINPLLNIILTGGTFRRETESFVGPIAVQNLD
                     SFNARLAFVGTDGFTIQRGMTTQFTEGAEIVRAMNKRAEITWLVADSSKFGKIGFVSV
                     LPLLAVHGIITDNGLPKEALEALQSEGLEVILA"
     gene            58183..59505
                     /locus_tag="PLS88_00210"
     CDS             58183..59505
                     /locus_tag="PLS88_00210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOU05816.1"
                     /translation="MAEKIIMPKLGNTVESSVILSWKVRPGDIVTKDTILCEIETDKA
                     TMEVPAGVDGTVLALLKKEGDDVPVLETIAIVGQPGEVWENTEVSPQASTASISPSAT
                     ASITSKEEKETTMPQKLHSEEPHSSPRARMAMYHLGIGQEAIDKGSGPGGRILEKDVI
                     EASIQEPSIQEAAIQEIPAQEAVIQEDSAKTPNAAQIQTTKEEPSIHERMEFQSIPLI
                     GIRKRIAGRMHDSVQTTAQYTEHGSADAEHLLALRARLKAQENPNLAHITIGDLVLMA
                     VIKTLPEFPEFNAHLEGNELRLFKPIHLGVAVDTPRGLMVPVIRNAQDLSLTGVSMEV
                     KRLAKACQSGRADPNSLSGSTFTVTNLGAFGVEWFTPIVNTPETAILGLCTIRPALVK
                     TDKGIETCMRIGLSLTVDHQVIDGAYAARFLRRLSEIIADIDIMPFLL"
     gene            59546..61015
                     /gene="lpdA"
                     /locus_tag="PLS88_00215"
     CDS             59546..61015
                     /gene="lpdA"
                     /locus_tag="PLS88_00215"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOU05817.1"
                     /translation="MTQYDVIILGSGPGGYLAAERLAAKKLKVALIEKAYVGGTCLNI
                     GCIPTKTLLNSAKLYAHLHEASSFGIESEGLQVNWQRTMAWKEEVSKKLRRSLELTLR
                     KAGVEIIRGTGKLTDGHSITVFPPAGESDGFRSSSTSASQSSGSGSLEFPLVIKGQKI
                     IIATGSSPIIPPIPGIKDNPAVLDSTGLLSVAEIPSRLCIIGGGVIGIEFASLFSSLG
                     SKVTVIEMMDEIVPVMDRQMAIELRKALKNVTFMLGTKVTELDGKKVLFENKNGEKGS
                     VEADIILLSVGRKANIEDWGAKESGLDIKNRAIATDDRMRTNLPGIWAIGDVTGKSQL
                     AHAAYRMAEVAVSDILAGQNAQHSDQIFVAETVPWALYSLPEAAGVGLTEQDARAKGY
                     DIQVVRMPYNVSGRFVAENGFSAPGSIKIIIEKSSERLLGIHLLGAYASEQIWGAALA
                     LERKLPISALRNMVFPHPTVSEVIREAAWSVQASGGTDQ"
     gene            61066..63522
                     /locus_tag="PLS88_00220"
     CDS             61066..63522
                     /locus_tag="PLS88_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU05818.1"
                     /translation="MPKSLPIKPEDVRKPGMLEIPSIPINQYKRNIKEERKRFGDDGL
                     IAMLHDMIVVREFETMLNTYKTMGSWEGIPYSYKGPAHLSIGQEAAVVGQAAALEPED
                     FIFGSHRSHGEILSKCLSAARKMDESKLISTMESWLDGETLSYAKRMPYVDTFSLARN
                     FIIFGTLGEIFARKAGFNRGMGGSMHAFFLPFGSMPNNAIVGGSADIALGSALYKRIN
                     KKPGIVIANIGDASMGCGPVWEAMMMASMDQYRTLWPESVGGAPPILFNFFNNFYGMG
                     GQTYGETMGFGVLARVGAGVNPEAMHSERVDGLDPLAVADAVLRKKKILLEGKGPVLL
                     DTITYRISGHSTSDASSYRTKEEIEEWRKNDSIDDFSKYLIENAILKESDIEALQKDI
                     HSTIYEAVKLATNDEECPRPTGALIESVMYSNKKELTFDARTPELLQAGEDNPRVQSI
                     SHRSRYAFDEEGKAIPKNKLYQFRDGIFEALLYHFKLDPTLAAWGEENRDWGGAFAVY
                     RGLTEALPYHRLFNSPISEAAIVGSGVGYALSGGRAVVELMYCDFLGRAGDEVFNQVS
                     KWQSMSAGLLKMPLVVRVSIGNKYGAQHSQDWSSLVAHIPGLKVYFPATPYDAKGMMH
                     LALSHTDPVIFLESQLLYDVGEQFEKGGVPKDYYEIPEGLPAIRKPGKDLTIATYGAT
                     LYRAMEAAKILEEKYGLSVEVMDLRFLTPFDYDPLVESVKKTGRLLLASDAVERGSYL
                     HTIASNVEQLAFDWLDAPVVVVGSRNWITPAAELESIFFPQPEWIVDAVHERILPLKG
                     HEPSTIQTTGDIARRYRIGV"
     gene            63567..64934
                     /locus_tag="PLS88_00225"
     CDS             63567..64934
                     /locus_tag="PLS88_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU05819.1"
                     /translation="MNDSKELERIINDARNEPVLRLEALAQLAEMMGQPAARSGESNN
                     HIHTCYSFSPYTPSGAALAARRAGLDVAGSVDHDSYAAASEMRAACALLDIGVVTGFE
                     LRVSLSEAARSFPEKTATMLTTRKLNNPDSIGIVYMTVQGIPAPALKKVEVFLSPIRA
                     ARYRRSALMAELANDILLSLGLPGIDFEKDVVSNSKYSEGGTITERHLLAAVSRSILS
                     QVEPGNELIKWLEENLDLVLTGSQKRNLSESQNPYLVYDLLGALKAGFLERIFIQPTE
                     ECVDVRDVISFANRIGAIPAYAYLGDVAESPTGDKRAEKFEDEILDELIPVIKELGFP
                     AITYMPPRNTKEQLLRLQGLCKKYNLMEISGVDINQPRQSFRCPELLLPEFRHLDDAT
                     WAMVAHEALSGFDLRFGLFSAENPLADMPITERICRYATVGRAIDFREPGSLEKQAKL
                     LFLEK"
     gene            64937..66178
                     /locus_tag="PLS88_00230"
     CDS             64937..66178
                     /locus_tag="PLS88_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU05820.1"
                     /translation="MNIIINPSSLAPIVRGFVLSLADYSCMVQSVEAAFSHAGEPVFH
                     SAHSDELSLIIDGLASGANMDDGLVASLLKKALEPFVTRIREGLAKGTIELSISNAKG
                     ESIAQYQLVPIAPLDSRIRLAVHRPGDPSLLSSSARSEVVKGKISFVTGGAQGFGAEI
                     ARGLALSGAIVWIADINLPGAERFARELSEEIGNQRVFPVEIDVSKEESVQKAFEIVA
                     QTCGGLDLVVSNAGILKAGSVLEQPAKEFRLVNEINYIGFFNVAQQAARLLRLQWLGA
                     PDWYTDIIQINSKSGLEGSNKNAAYAGSKFGGIGLVQSFALELIEYRIKVNAICPGNF
                     FEGPLWSDPQKGLFVQYLNSGKVPGAKTIEDVRAYYEAKIPMRRGCYGQDVIKAIYYL
                     VEQLYETGQALPVTGGQVMLN"
     gene            66263..67771
                     /locus_tag="PLS88_00235"
     CDS             66263..67771
                     /locus_tag="PLS88_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOU05821.1"
                     /translation="MTEAIAVLDIGMTNKKVVLYSNELALIGEKRKVFPPLMRDGIET
                     HDLKAMEEWFLASLGEFARSFSIGAIAVTTHGATFVCTDEKGNPVAPCIYYTYEPSLE
                     FHERFYERAGKPEDLQAITGTPNFSALINPAKGLFFLKENYPLEFAKAHWVLPYPQYW
                     GMRLTGIAAAESTYLGCHTYLYDWQKNDYSSVADMLGIRAKLPYPIRQAWEVLGTISP
                     DIAARTGLSLDTLVTLGIHDSNASILPHLASKVSHDFVLNSTGTWCVLMHPVDKYGFE
                     PDELGKVVFFNRSAWNTPIKTAIFLGGKEYETWMDIIAKSSGSKSSRQLPKPDKKDYL
                     RVLEECNCFILPEIVPGSGQFPGSVARAVQGGISYPLSDIEEGKYKIAFLQDAKIAQA
                     VLNLSIVLQTEVALQRTGIKPGTEILTEGGFRSNTDYNMLLGSCFPGNAVYLTDLQEA
                     TSFGAAMTALTALKKIDPRALSRYIHIEKVLVPSMFAPERLSAYRNAWHAQI"
     gene            67806..69068
                     /locus_tag="PLS88_00240"
     CDS             67806..69068
                     /locus_tag="PLS88_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOU05822.1"
                     /translation="MKTKAVRIYGVNDLRLEEFDLPPLKDDEILARVVSDSICMSSHK
                     LAMQGDAHKRVRAPLAQNPIIIGHEFCGELVEVGAKWAHKFKPGMRFAIQPALNYKGT
                     LWAPGYSYPYIGGDATYIIIPNEVMEMDCLFEYKADAFFMGSLSEPVSCIVGAYHAQY
                     HTQPGSYVHEMGIKPGGSLALLASVGPMGLGAIDYAIHGDIKPARIIVTDIDQARLDR
                     AQKLISVESAQKEGIELIYVNTKDLPDPVATLRELNGGKLFDDVFVFAPVRSLVEMAD
                     KLLGKDGCLNFFAGPTDPNFSANFNFYNVHYESHHLVGTSGGNTDDIRESLELMAKGL
                     LRPEAMITHVGGLNAVAETTINLDKIPGGKKLIYTHKKLDLVAIADFAARGTTDPFYA
                     ELARITQAHNGLWCKEAEDYLLAHAPEI"
     gene            69116..>69791
                     /locus_tag="PLS88_00245"
     CDS             69116..>69791
                     /locus_tag="PLS88_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOU05823.1"
                     /translation="MNWLNEIIGSLLSVIGLNTSSQISGSVQFFLYDVIKITLLLCIL
                     IFIVSYIQSYFPPERSRKIMGRFHGFWASCVAALLGTVTPFCSCSSIPLFMGFTSAGL
                     PLGVTFSFLISSPMVDLGSLVLLMSIFGTKVAVVYVLLGLVVAISGGMLIEHLHMESY
                     VEDFIKNVSSVEIDSPDPTQRERLALSKNQVASTFKKVFPYILLGVGIGTVIHNWIPE
                     AWISSVL"
BASE COUNT        18797 a        16484 c        16171 g        18339 t
ORIGIN      
        1 attctccatg attccgaatt cattgagaaa tgtgcccgaa ccggtcttga tgatggagaa
       61 tacgttttcg atatctaccg tacctggacg aataaatggg tataaactca accaattata
      121 tatcgaacaa tcaatcatac tttaagtaat tgcgctatta taggaatcgt agcagccgat
      181 aacacagtgg aaataaagac tattttcgat gcaagcaaga catctgcatc atggcgttct
      241 gcgaagataa cggtttgagc agcggcaggc atcgccacca aaagattggc tacctttgtc
      301 ggaaaatcgt cctgctttaa tattttcata aaaattataa aaatagccgg caaaaccaga
      361 agcctcataa aagatcctat ccatacttcc attccagaga ataagccttt tacaccaaga
      421 ctggccaatg tcgctcccac aacgatcata gataaaggcg tcgtacagtt cgcaatgaga
      481 gctatagcat aactgattgt ccgaggcatc gaaaaaggca agatccaaag aataaagcct
      541 atagctaccg cgattattcc tggattgaga agcatgtctc ttatcttagt cttcttagtt
      601 ttttgatcgc tgttttcgcc tcgagacagg agagcaacac catagctcca ggtgaaaata
      661 ttgaaaatca tcacatagat tgaaacatac ataagactgg aaggaccaaa aatgctctcg
      721 gcaagaggga agcccataaa tccacaatta gaaaacactg ttgcatagat taggattttt
      781 tgtttggttc tttccatttt tgaaaagaga atttccgaaa gagcgataga aattccgtga
      841 atcgcaatag catatatcgc tacttcgatt agatcttttg ccgcggaaaa aggaatgctt
      901 ctatcgaaac ttgagattat ggtgcaaggg agagctattg tgagtactag atttgaaacg
      961 gctctaattc cattttcctc tagcagcttt gtttttctcg tgacataacc aactgctatc
     1021 aagagaaaaa tggaaaaaat acgcgataag gccataattg agtgcttttt tattctatag
     1081 tgttttcaag ccgaccgata ccttctattt cgatgcatat gctatctccc ttttgcatgg
     1141 gtcctactcc agaaggagtg ccggtcatga tcacatctcc tggatttaga gtcataatct
     1201 gggagatata ttctattaaa gaaggaacat caaaaatcag atttgaggtc gatgagactt
     1261 gcttaggttc attattgaga taggccttta gtttcaaaga agaaggatct aggtcagttt
     1321 ctatccatgg gcccaatggc ataaaggtat cgaaggattt ggccactgtc cattgaccat
     1381 ctttgggctg cagatctcgt gctgtcacat cgttaccgca tgtataacca agcacgtaat
     1441 caagcgcata tttggcatca atgttttttg ctgttttgcc tattacgacc acaagctcgg
     1501 cttcataatc tacctgtttc gacaatcttg gatagtgtat gggcttccca gggcccaaaa
     1561 gcgttgtcga tggtttaatg aacaacactg gagaagatgg aatttttagc ccgaattctt
     1621 cggcatgatc cctgtaattg agaccaacgc atacggcttt tgtcggaaca caaggagctt
     1681 ccagatatac gctagaaaat ggaacttttg cttcttctcg gtcaaaatca attcgctttt
     1741 tcttgtcatg aatccgaatc tgaggaagat ccttcgaaag gacgaccaga tcatcttcta
     1801 tgcggccgta atatatggca tctttataaa tgcatcttat tattctcatt tttgctccat
     1861 aaaatctcaa attggcatta caacgagtgc aaagtgggat tcactatatc ataagcagtt
     1921 atcgaataca attattaata ttctttggct atctttgctg caaggcgagc attgttcagc
     1981 accagcgcga tgttcgagtc gagggaatcc cctcctgtca aatccgcaac tctagaaagc
     2041 agatacggag tgacttcagc acctttcagc cctttgtgct cagattcttc gattgctttt
     2101 tggattgctc cctctatcgt cgtttcgctc atggcatatt gagaaggaat aggatttgcg
     2161 atgacaactc cgcctttcaa gccagattcc catttggcac gaaggatcat tgctatctct
     2221 cgaggagtat cgactctaag cgaaagttct acgccagaat gttgcgtata gaaagcagga
     2281 agctccgatg ttccgtagcc gatcaccggg actcctttgg tttctagata ttccatggtc
     2341 ttgggaatgt ccagaattgc ctttgcgcct gcgcaaacca ctgctacatc ggtgcgcgct
     2401 aattcttcta ggtcagcaga tacatcccag gatttttccg ctccacgatg gactccacct
     2461 ataccgcctg tggcaaacac atggattgct gccatctcgg cgattatcat tgttcccgct
     2521 actgtggttg cgccatttcc tccaaatgcc ataagtgccg gaaagtccct tcgggacgct
     2581 ttgatgggct tgagagaacc attcgcgagt gaaacgagct cttcgcgtgt caaaccgact
     2641 ttcagccttc cttttattat cgcgatagtg gcggggatcg ccccttcttc tcgaaccgca
     2701 ttttctactt ttagggcagt ctccagattt tgcggccagg gcataccgtg tgtaattatc
     2761 gtcgattcta gagcaactac gggtatcccc gtagtaattg catcagcaac ttcttctgaa
     2821 atatcaagat acttgttcat ttatgattct ccgtttggat tgcattaata agctcaccta
     2881 tcagcttgga tgtttcagga tagcagattc ttgtcgtttc gaacaggttg cgctgattta
     2941 gatgaggatt aaccggtcga ttcgaggagc tagcatagag tgcggaacta agtgctagtt
     3001 ttgctttgtc tctgggagaa atagaccagg agctatctcc atacttttct tttaatcgtc
     3061 tctcaagcaa tgaaatccaa acaagagcag cacatgctgc atcgccggcg cctgaacgat
     3121 ttataacatg agggcaaagc ttgaaagagt tcaacggaat atgctcgata atctctctgt
     3181 ctataaaact aatgccctca gttcctttag atatatacag ctcttttggc agaagagact
     3241 ttttcctaag agataaagct atctcttcta tagaagcatt ttcttcaagg ccagctattc
     3301 tggcagattc actaacattg ggctttgcta tgtcgaattt tccaaagcat gagacaaatt
     3361 tttccgactt ttttgtagaa acagggtcgg caataagcaa aggcctagaa attgcgtctg
     3421 gttcttgaga cttttgcttt atagaagata tatgaggctt cttttcatct tctaagacac
     3481 cataattctc ggcgatccat tcgatacaat cctttggaat attcccatcc accacaattg
     3541 cttcagcgta atcgaacaac gatttttgag ctttaagctg attgacctgc atcttttcga
     3601 tagcacgcat atccgcaacg gcacccataa ggtttccatc cctgtctaga atacaaagat
     3661 agacagataa aggcgcttcc ttgattctga ggctatgagc aagactaatc cccaacttct
     3721 cggtacgaga gactagccca tcccagcttg aagaatctcc aaccacagtt atcagctcta
     3781 catccaaccc aaggcgcacg aggttctcag ctatattccg tccgacacct cctaggcttc
     3841 tatgaatttg cccggggttt gagtcttcgg gccgatagtc aaagctgcaa aaagcctgaa
     3901 tatccaaatt cagacctcct acaacgacac aatatggctt tctatgtcta tctattttca
     3961 aggtctctcg ccctccacca agatttgcaa atatgctata ttatcatcag aaatcttatt
     4021 tagcgaggca taaggtggat caaatattcg ataggcttga gatgctggtt cgcgcatgga
     4081 tgtcctccac gtttggtgag ggtttggggc gaacaaggtt tcgagactca gagacattcg
     4141 aagatccgga tcttgccgac gcatgggatg agctcgaatc atacctggat ccttttaagg
     4201 cagacaaaaa gcacaaagcc cgaacgaatt ggaggtctta tacttaccgg tcttacgata
     4261 cgggtgagac tcttgaagaa gaaaccaaaa gaaaggctgt ggaagaggct tacagatttc
     4321 taggattaga accctatgct ccttttgcgc aggtaaaggt tcgatataag aagctcttaa
     4381 aagagcatca tccagatcgg catactaagt ccgccacgga taacgcggaa gcaaatgaga
     4441 taagtgctaa gattaacgcg gcctaccaac ttatcgaagc atgggaagaa tctcaagcta
     4501 aaagtttcag ataaggatac agaagaataa aatggaaaac aaagcccctt tgtatatcct
     4561 cgatgtctat gcaattatct atcgttctta ttttgcattt atccgaaagc ctttacgaaa
     4621 ttccaaaggg gaaaatgtat cggcgttgtt cgggtttttc cgttttcttt tttctctatt
     4681 cgaacaaaaa aaaccggagc tctttgcagc cgctctagat tctattggac ctacatttcg
     4741 ccatacgcag tttgccactt ataaggcaac tcgtcagaaa acgccagagg acctaacagc
     4801 acagattcca aaaatcgaag cgattcttca ggcattggaa gttccagcga ttcgatccga
     4861 aggatatgaa gccgacgata taatagcaac tttagcaaat caatgtcgga aagaaggtcg
     4921 accatgcttt attgtttctg gcgacaagga tttgttgcaa cttgtaggag ataagatatt
     4981 cgcgcttcgg ccttcggaaa acttctcatt tcgggagctg gatgcccaag gagtttttaa
     5041 cgaatgggga gtaagacccg atcaaatccg agatttcctt gcacttacag gagatgcctc
     5101 ggataatgtg ccgggggtaa agggcattgg agaaaaaaca gctcagaagc taatttctaa
     5161 attcggctca ctagaagcta tatataataa tttgactgcg atagagccag aatctctgcg
     5221 caaaaagctc gaagaaggaa gagagagtgc atttttatcc aaagagctta tatctctgtg
     5281 ctatgatgtt cctgcttgcc ctaatgatgt ctctctattg aaaccttctt tgaatatcga
     5341 agcagcggct cctctttttt tgagagaaga tatcaagagc cttattcctg atgaattgcg
     5401 taggcaattg cttgagaaat ccacaactag aaaagaagtt gcttcagctg cgagtttcca
     5461 ggaaatttca gggcgagcgc aacaggtaca ggctagatct tctacgcaag ttatgccttc
     5521 tacgcaagct gtgcattctt cgcaagcttc gctttcttca gaggctgcag ctaaaggaat
     5581 aactcaaggg gcagcttcta tggtacctat agagaatact tcaggattat cgcctagcca
     5641 actaatgcaa gcgaattatg aggcagtcct gtcgatcgaa aaactcgata ggatcatcga
     5701 tgaggctatt gaacgagctg tctgtgcttt tgataccgag accgattctc tagatgtgct
     5761 taaggcggga cttgtcggtt tttctcttgc tttcgatgct gattcagcct ggtatgtgcc
     5821 cctaaagagt cctgattcag aatgcattcc aaaggacctg gccctttcgt ctttatcgag
     5881 gcttatgaag gagccacaaa tgctccttat tgggcacaac ctaaagttcg atataaacgt
     5941 tctctacact gcgggccttg aagtaaatcc aacttgcttt gacacgatga tagccgcatg
     6001 ggttctggat gtagaggctc cttctttttc gctatcttcg gttgctaaac gttttctagg
     6061 gatttatgga ctagaatatg aagatgtagt accgaaaggc cttacctttg ccgatgtgcc
     6121 tttggctagc gcaactcgct atgcatcgga agatgcgtac ttttcctttc tactctatca
     6181 atttctctct aaggaaattg agacgcataa tctgcataat atattctatg atatagagat
     6241 gcccttattg ccaatccttg ctgaaatgga acgaaccggg attcgcgtcg aggcagggcg
     6301 ccttcgtgcc tatggtaaag aactacagaa agagcttaca gagatcgaaa ccaaagtctt
     6361 ccagctggtt ggccatgagt tcaatttggc ttctcctaaa caactagccg aagtgctatt
     6421 ctccgagagg aaactacctg tacaaaagcg aacaaaaaca ggcttttcta ccgatacttc
     6481 tgttctagaa gagcttgcac ctttggatcc cgttcctcag ctcattctta ggcaaaggct
     6541 cttgaccaaa ctaaagacga catatatcga acctctcgca gagttggcaa cggcgcaagg
     6601 acgaattcat actacctttg tgcagactgg cgccgcaacc ggaaggcttt ccagcagaga
     6661 tccaaacctc caaaatatcc ctatacgcga agaagaaggc agaaaaataa gagaggcttt
     6721 tattgccgaa ccagggcatt tgcttgtttc tgccgattat tcgcaaatcg agctagcagt
     6781 tctggctcac ctctccaaag atcctaatct tatcaaagcc ttcaaagaag gagtcgatat
     6841 ccataagcgg actgcttcct ttatctttgg aatacctgag gatcaggtcg atgcagatcg
     6901 aagaagaatt gctaagacta tcaattttgg ggtcatatat ggcatgagcg catttagact
     6961 tgcgcgagat ctaggtattt cgaacagcct agcaaaggaa tttatcgatt catatttttc
     7021 gacttattca ggagttgcag attttattca aaaaacgatc aaagaagccc aagaaaaagg
     7081 cgtcactttt accttatttg ggcgcaggcg gcagattctt ggaatcaatt ctaaaaataa
     7141 aaccgagcag caagcagcac agcgagttgc cgtcaataca ccaatacagg ggactgctgc
     7201 agacatagta aaaatagcta tgatcaaagt acatcgaagg cttaggaagg aaatgccaga
     7261 agtgcatatg ctgctgcaag tacacgacga acttgtattt gaagcgccac aagctctggt
     7321 ggaaggagcc atgaagctta tcaaagagga aatggaacat gctgtactgc tcgatattcc
     7381 acttagggta agcatggaat cggctccatc ttggggcgag atgcatttgt aattcaaaag
     7441 gaaaggtcga tgttcatact tggcctcact ggaggttatt gcgtcggaaa aaacgaagtc
     7501 gcaagcatac tcgaaaaaag tggctggaaa gtcacggatg tcgataagct tggccatgtg
     7561 gcgcttcccc ttgtatgcaa tgagcttatc gatgcctttg gcgactctat tctaagagaa
     7621 gatggttcca tagaccgaaa aaaacttggt tcgctggtat tcaataatag agagcgattg
     7681 acgagtctcg aaaatattgt ccatcccgca atgctttctt tactagataa ggaaatagag
     7741 aaagcgcaac agaattcaat acaaaaatta tgcataaatg cggcattgct ctatcgtttt
     7801 ccacagcttg attcatgcga tgcggtcatc gaagttagag cgccacttta tctaagaata
     7861 gatcgagcga tacaaagaga tcatctgtct ttcgatgaag cactccatag aatctcgagc
     7921 cagaaagagc tttggagaaa gagatctgat gtcagtgtcc ctatatatat gctttggaat
     7981 ctaaaatctc tagaggaact cgaggccgat gctcttgaat tgctaaaaaa aatagaaaat
     8041 gaaataaagg gaaagcggag ctttacctaa taatttacct tgccgatatt aattaaatag
     8101 aggtctagaa atgtcggatc gttcaaaaaa attgctatgg attgctgtag cggtaagtgg
     8161 cttcgcattg attgtccttc ttgctgtatt tattatcttt ccacccaatc aattgtctga
     8221 atccaaacct tttgacctga caggcaggtc gcctgccaaa gaggtatctc caaatgactt
     8281 ttctgccttt ccaagtatcg tcatccaatc ttcagaagga gaaggtcagg cttccagcac
     8341 ttccaaagat gaaaagacag aaccccaaaa accgcagagc aatgtgatta tcgttcctgc
     8401 tccttcctca aatcaaaaaa ccgctgaaac tccttcgcaa ccaattgagt cgcaatcgca
     8461 aaagactgct gttgcaccaa aaacgtcaca aacatctcaa acatcgcaaa caccagcaca
     8521 gagctctgca aagacaagcc cagcaacaac acctagcgcc tcatcaacac agaaaacaac
     8581 ccagccaaca ccttctgctg caccaaaaac tgactcatcg agcacggaat attggattca
     8641 agttggcagt ttttcgcaac aagatactgc ggaaaaactt cgcagtgaat atataactcg
     8701 tggcatgaca gccatcattg cgcaaaagga tgtcggcggt aaaacctatt atcaagtcaa
     8761 ggttgggcct tatgcatcat cggatgaggc aaaaaaatgg cttcttacgg taaaatccgt
     8821 caaaggcgca agccaggatg cctttgttac aagccggtaa gaattcgaca atatacagag
     8881 cgctccgtac aaagcactcc atgataaatt tgtttgacag caggcgattt ggaggctaaa
     8941 cttttttagg cacttaatgc caacggaatt ttccgttttt accaatcaag caaaggagtc
     9001 gccaatgaga aagatcattc tcggtctgct tgctcttctt atggcagttt acctcgtgct
     9061 gccagccacg gctcaggcaa aaaaacctct aaaggtcgcc tttgtatata tcggcccgcc
     9121 aggtgatctc ggttggacct atgagcatga acgaggacgg ctagctctac agaaatattt
     9181 cggcgataaa atcgaaacca aatatgtaga aaatgttcca gagggcccgg atgcagagcg
     9241 tatcattcgt caattcgcta ttcagggatt cgatgttatc ttcactacaa gttttggtta
     9301 tatggaccca acgtatgctg tagctcagga attcccaaat atagttttcg aacactgctc
     9361 aggatataag acggctccaa atatggctac atactttgga cgaatcgaag aagcccgcta
     9421 cctaactggc attattgctg gccgcatgac caaaaccaat aagatcggtt atgtagcagc
     9481 cttcggaatt cctgaagtcg tgaggggcct aaatggattc gctctaggag tacggtctgt
     9541 caatccaaat gcaaagatcc aggttgtctg gacgaatacc tggtatgacc ccgtaaaaga
     9601 aagagaagct gcaatagcac ttctcgacgc gggctgcgat atcattgcac aacaccagga
     9661 tactacagag cctcaaaaag cagcacagga gcgaggcaag ctctctatcg gctatgactc
     9721 agatatggca aaattcgtag gagatactgt tcttgcaagc gcaatttgga actgggaaac
     9781 ctactatatc aagactatcc aagaggtatt gaacggaact tggaagacac acgcctactg
     9841 gggtggattg aaagatgata ttgtaaagct ttcggaccta agcccgcgcg tgcccgcaaa
     9901 tgttaggaaa gaagtggagg atgcaaagaa aaagatattg ggcggatgga gtatctttac
     9961 tggccccata aaggatcaga atgggaagat tgtctacaag gcaggcgaag tcatccccga
    10021 cgacaaacag ctttcaatgg attggtttgt tgaaggggta gtaggtaagg ttcagtgatt
    10081 attttctgcc ggcatgtttg ctcaaagcaa tgcatgccgg ctttttttat ctataagatc
    10141 tatgaatgat tcgaatattc cgaaagtcga aatgagacgc atttccaagt cattccctgg
    10201 agttgttgca aatgattgta ttgatttgaa gctccataaa ggagaggttt tagcgcttct
    10261 gggagagaat ggcgcaggaa agagcacttt gatgaatata ttgtgtggcc tgtataggcc
    10321 ggatcaaggt gaaatataca tagacggaca gcaagtcgaa atccattctc cgcgagatgc
    10381 tcaaaaatat ggaataggga tggtgcacca gagtttcaag ctcgtagatt cgatgaatgt
    10441 tcttgaaaat atcattctag gcctccgcaa tgagcccttc gtaccagata tgaagcttgt
    10501 tcgcaagcag ctcatggaat tagcatctca ataccacctc agtgtcgacc cagatgcttg
    10561 tatatggcaa ctatccgtcg gagagcagca gcgtgtggag atcctaaaac ttctttatag
    10621 gaatgctgaa gtcttgatat tggatgaacc cactgccgtc cttactccac aagaggctca
    10681 tgagctgaat cgtgtcatca agaccatgaa gcaagaaggc aagtcagcaa tattcataac
    10741 tcataaaatg gaagaagtga ttgagttttc agaccgcgtc atggtgctgc aaaaaggaag
    10801 agtcgttgca gaaacaccta cttcagagac gagtcctaaa gaactagcgc gaatgatggt
    10861 aggacgcgaa gtcctctttc aaattgaaag aaaaccctat agccctggaa aagcaatatt
    10921 ggagctctct cacattgaag cacttggaga tagaggctta cctgctctca aggacctatc
    10981 atttagccta aaggcaggag agatactcgg tattgccgga gtcgatggta atggacagag
    11041 agagctagca gaggtcgtaa ctgggctgcg aaagtctaca aaaggaacgc taaagattgc
    11101 agataaagat atgaccaaca aatctccact cgagattatt cgagagggag tagcacacat
    11161 acctcaggat agagcatccg ttggagcggt cggagatctc agtgtcgcat cgaatcttgc
    11221 catgaagcaa taccgctcca agccacttgc tcagggaatc atccttttgc ccaaaagaat
    11281 catcgatttt gcccgcaagt tgatagataa atttcgtatt gccaccccct cgcccaatac
    11341 tcaagtcaaa ttcctctccg gcggaaatgt gcagaagacg attctggcta gggagatcgg
    11401 cgcagcacgc tcgttgataa ttgcagtcta tccatctcga gggctcgatg taggagctac
    11461 agagtcagta aggcgtcaac taatcaatat gcgagatgaa gggcttggtg tcctactttt
    11521 ctcggaggat ctagatgagc ttatgcaggt ctcggaccgc atagccgtta ttttcgaagg
    11581 gaggatcatg gatattttcg acgctgaaaa tatagatatc gaacatattg gaatgctgat
    11641 ggcaggcatg aagacggagg tctattgatg aggttcgtat tcgaaaaacg aaaggcgaca
    11701 tcgatttcag cccttatcct cgtccctctg atatcatttg cagtctctct gtgcctgact
    11761 gccctattgc tcacaatttt cggagccgat cctctcaaaa cctattctgc aatggcaatt
    11821 ggtgcttttg ggtctttgca tggttttgca gaaacacttg taaaagccat acctctaatg
    11881 atgacaggcc tcggagtggc aattgctttt cagttaaagt tttggaatat aggtgccgaa
    11941 ggtcagctta cgctgggcgg cgtagccgcg gcgggagtgg ctctttttct agagcaatac
    12001 ctgccaggtc cctttcttct tgtgggcgca ttattaggag gcttacttgc aggtgcactt
    12061 tgggcaggca ttcctgcgat tctcaagacc tctctaaatg tcgacgagac tcttgtcacc
    12121 cttatgatga attatatcgc cattttgttc tcagaatacc tctattacgg cccctggcga
    12181 gatcctaagg gatatggatt ccccggatcg cgtatgtttt catctaatgc ttggcttcca
    12241 cgaatagtgg gacgcgcgca tatcggcatt ttaatagctt tgttcgttgc ccttgtcctg
    12301 tggtttattc ttaggcggac tcgctggggc ttcgagctgc agatcattgg tgcaagccaa
    12361 caagcagcaa ggtatcaagg agtcgccgtt gaacgaaata tcgtccttgc aattacgctt
    12421 tccggggctc tctgcggact agcaggagca ttcgaggtaa cagggatatc acatcgttta
    12481 cagcaaggcc tttctattgg ttatggatat acagcgataa tagttgcatg gatgagtcaa
    12541 ctcaatcctc ttgtgattcc ttttgtagcg gtagcgctgg ccgcgctatc tgtaggaggc
    12601 gatcaggtgc agatgataat ggggctacct gcggctatgg gtaccgtgat gcaaggtctc
    12661 atactattcc ctatgctagc aggctccctg ttcactgaat acaggcttct ggcagtaaaa
    12721 gacaataaga gcaacttgcc tggatgaaac ttaggagtat tgtatggatt ttgtcacttc
    12781 aattctgacg attaccatcc gtgcaggaac gagccttacc attgctacta ttggagaaat
    12841 cttcaccgaa cgcaccggta ttctaaatct cggtgtcgag ggaatcatgc ttatgggcgc
    12901 cttgtctgct tttactgctg tcttttatag ccacagcatc atgctgggaa tcttggtagc
    12961 aatggtgatc ggaggtctgc tcgccttaat acatgctttt cttaccacga caatgagggc
    13021 aaatcaagtt gtctcggggc tttcgataac gctctttgga acagggtttt catcatttct
    13081 gggacaaagg cttggaccag aatccaacaa tttcaggtta gtaggcctta gagcagagcg
    13141 aattctgccc tttgctccaa aatgggcaaa agacattccg attctttcgg catttttgaa
    13201 tcaagacata attacctatc tcgtatacct ccttattccc ctagcatggt tttttcttta
    13261 taagacaaga ccaggcctat ggcttcgttc cgtcggagaa gatccctata cggccgatgc
    13321 aatgggaatc gatgttacaa aaactagata tttctatact attattggtg gaatgctaat
    13381 agctttggga ggagcccatc tttctctttc ttacactcct ggatggtcgg aaaatattac
    13441 tggcggaaga ggttggattg tgattgcgct cgttatcttt tcgatgtgga atcctgcacg
    13501 cgcagtgtgg ggatccctat tgttcggagg aatcaacgca gtccagtttc gtcttcaggc
    13561 atcaggaacg agcatacctg cgaactttct caacatgttg ccatatattg gcactataat
    13621 agtgcttgta gctattacat ggtgggaagc cctgtccaaa aaagtgggcg cgcctgcagc
    13681 gcttggatcc tcatatatga gggaggaaaa atgaaaaagg acatcataga cgccgcgatt
    13741 ggaaaagtac aatgcgacgt caattattcc aattgtatcc tagtagatgt cctttctggc
    13801 cgtttgctca aggattctgt tgtttcgatt aagaacggtt atgtagctgg aatcaacgat
    13861 gggctttcgg caaaggaaac aatagacctc agaggaatgt atcttgcacc tggtcttgtc
    13921 gatggccatg tacacataga gtcctcactt ctcactccgg ccgaatatgc aaaagtcgta
    13981 atacctcacg gaaccaccac tgtaattgca gatcctcatg agattgccaa cgtgatgggc
    14041 tatgatggta tgcgctatat gctcaatgtt tcggaaaaca ttcccctaga tgtttatttt
    14101 atggttccat cgtgcgtacc ggcaactgat ttcgatactg ctggagcggc gctctatgcc
    14161 tctgatatgc atcagttttt acaggagcct cgcgtactcg ggcttggcga agtgatgaac
    14221 tatccaggag ttttggcgca ggatccgcag cttatggata agatcgcact attcaagtca
    14281 aatggaaggc ctattgacgg acattctcct gggctatcgg gcgcccgcct ctcggcatac
    14341 attgccgcag gaataggctc tgaccatgag tgtaccagcg cagaagaagc attggagaag
    14401 gtgggcaagg gaatgtacat aatgctgagg gaaggctcta cggcaaagga tttgcgcaac
    14461 ttgttatcgg cagttagacg agaaaacgca gggcgcttca tgctatgttc agatgaccgt
    14521 cattgcaacg atctacgcga tgagggccac atagacttct cgctaaggct tatgttggag
    14581 gcaggcgtcg atcctataga tgcaattcgc atagcttcga gcaatgcagc tagatggttt
    14641 ggaattcctt ctcaaggtgc tattgctcca ggttataagg ccgattttat cgtgttcccc
    14701 tcatttgaat ccttcgagat caaagcggtc gtcaaaaacg gcaaaattgt tgcaaaagat
    14761 ggttctctga tcgaagactt ttctggagct ccaattgcga tccgagattc ggtcaatatc
    14821 aaatggttga ctgccgaaga cttcataatt cctgataaag ggtctccggt gagagtaatc
    14881 aaagtccaac aaaattcgct tataaccatc catggcatag aggagcttcc tttcgagaat
    14941 ggagcactcg tttcagatct cgaacgcgac atactcaaga tatttgtcat agagagacat
    15001 acaggctcgg ggaatattgg aaaaggcttc attcagggac ttggtcttaa acgcggagct
    15061 ataggcagca ctattagcca tgattcgcat catatgataa tcgccggcgt cgatgattta
    15121 tcgatcttca aggcagccag gcatctcaac aaaatcaagg gcggcttagt ctatgcagtg
    15181 ggagatcaaa tcttgctgga cctgcctttg cctgttgcag gtctcatgag cgataaaccg
    15241 gccgactttg ttataaaaaa gctgagcgat ttcgatagac tcttccagaa ggaaggtctg
    15301 acagcgactt ctcctcttat gacgctctcg tttatggccc ttccagttat tccaagcctc
    15361 aagattacag acaaaggtct tgtcgatgtc gaaaagttca aaaaagtatc gctctacgct
    15421 gacaaaatag tcaaagaaaa acactgattc ggtatcttgg cagcctttat gcttaaggca
    15481 agtcctttta aaaaggttgc ctttttccat aaacagagga aagcaagagg agggatacaa
    15541 gcgctcgggt ccagcgtcat gaatgccctt ccgaaaaatc tatgatataa ttttcgatta
    15601 gggagagcaa aatggcttat ttgcgcataa aggtcaacaa cacatggcat gatattcatt
    15661 catctgaagg aacgcccgca atcgatgtaa ttcgaggcga acttggcctt atggcaacaa
    15721 aggaaggttg tcgcgagggc gattgtggcg catgtgctgt attgattgga gaatatgcag
    15781 ctagtcaact gcgatatcga gcgcttcctt cgtgcctact tgcgattggc gagctcaaag
    15841 gcaaacatct tattacgctc gaaggcttaa tagagggagc tcaagacggt cttacaccag
    15901 tcatgcgcgc ctttttagat gagaatgcaa gtcaatgcgg gttttgtacg cctggattca
    15961 tcatcgcctt gacttcttgg ctggcggaac cgaaaccact cgaccttgca ggcgctttgc
    16021 aggccataga tggcaacttg tgccgatgta ctgggtatgc tgcaattcga agagcagccc
    16081 ttaggcttat cgagcaattc aaaggcctgc ccatcgatcc agaggctagg ctgaatgcgc
    16141 tagtgggtgt tcatgtgctt cctttatcgg tcagcgaatt tgcagctgaa atatccaggc
    16201 aattttcaca gaactcgata gaaaaaaaga accctcaaat agcgccaaaa gagagaataa
    16261 catctaatgg cattgtcatc ttaggcggag gaacagacta ttatgtaaag aaccccgagc
    16321 caggcgaaga cttttcgcca ttcttgattc gaatgaggcc ggaattcaaa gagattgctt
    16381 acttagagga aaaaggacaa aagtggctcg aaatcggtgc tgccgttacg gtgagagact
    16441 tttttgcctc agagcttgta agacaagaag ttccgggaat cgagcaattc gaaacgatgt
    16501 ttgcaagcac cttgatacgc aatctcgcta caattggcgg gaatatcgtc aatgcctcgc
    16561 cagtagcgga tatcacggca atgctattgg ctctcgacgc agaactcgtc atttcatcgc
    16621 aggaagaaga gggatttggc acatctataa cggcgcgtac ttgtaagctg gaagaattct
    16681 ttttgggata caagacaatc gatctaaaac caacagaagt catcagagct atcaggattc
    16741 catgctgtgt cgaccccaag acacggaaat tctcctttga aaaggcaagc aaacgcaaaa
    16801 atctcgatat cgccgccgta aatacggcca tatcttttag aatcgaggag caaaggttca
    16861 aggatgtaca cataagcctt ggaggagtcg cgcctatacc aatagtcagc aaagcagcaa
    16921 tggagctgct cgaaggttca ttctgcccta tctcagatgg gaaaacgatg gccgagcttg
    16981 caaagaaaac ggcaagcttg gctgaggctt cggtaaagcc cataagcgat gtgcgcggat
    17041 ctgaagaata tcggcgcaga atggtctata ggctcatgct tgcacatttt gtgcgcctct
    17101 ttgaagatag cggtgttgcg gaggagctat ttccatgaat aataaagata tacttgagca
    17161 ggacgaattc cgggctcggg aggctaattc ggacatgagt cttaaatggg caggtcgagc
    17221 ggaattggaa agcggctcgt cgggcttaat acccgaatcg ctaccaaatg ttctggggca
    17281 aactcaatat attgatgata ttccgcgacc agcaggttgc ctccaggccg tcgtcgttct
    17341 atcgcagagc gcacatggaa aaatccactc tatccacata gagaaggcta tcgagctcga
    17401 cgcttctgta cgggtcatcc tggcaaaaga catccctgga accaatcaga ttgggtttaa
    17461 caagcccgac gagcagctcc ttccagagga ggagtggcat tattggggcc agcctttggc
    17521 aattgttgtg gcgacttctc gcagtcttgc aagaaaagcc gcaagccttg tgcgcatcga
    17581 aggagaaaac cttcctgcaa tcatagatgc aagggaagcg gcaaccaagg gagattttat
    17641 ctttccaccg cggacaatag cttgtggaga tgtgctcgag gcattttcaa aatgtgcctt
    17701 tgtcgtcgaa ggcagagtag attcgggtgg gcaagaacat gtctatctcg aaacccaggg
    17761 agcaatcgct caagttatag atggtcgtag agtacatgtc atttcgagta cacaggggcc
    17821 aacgggcgta caaagagcgg ttgctcaagc gctgggctta cccatgaaca tggtggaagt
    17881 cgaggcaaga cgcctaggtg gagcatttgg cggcaaggaa gatcaaggag ccaattgggc
    17941 atgcatggct gccgtcgctt cctgggttac gggaaagcca gtcgaaatat acctcaaccg
    18001 caaggatgat atgcgggcca caggcaagcg tcatccttat tccacagatt ttcgccttgg
    18061 cgcagatgcg aacggcaaac tcatggcttt cgaggcagac tactaccaga attctggctc
    18121 gacctgcgat ctatcggcgg caattcttgc acgcactgtg ctgcatgcta cgggtgccta
    18181 tcagatcccg aatgttagag taactggcta tatgtgccat acgaacctgc cctctttcac
    18241 cgccttcagg ggctttggag ggcctcaagc gttctttgtt atcgaatcgg caatcgacgc
    18301 gcttgcacaa aagatgggaa tcgatccagt agaaataaag cggaacaatc tcttccaaga
    18361 aggcgacgtg acctattatg gcatgactat ggagcgagtg cgcgcatcgg actcggttga
    18421 gcagcttctc cgcaaggtcg attatcctgc gctaaaagct agaattgcag agttcaatgc
    18481 ctcgcatcga ctaaaaaaga agggccttgg ccttattccc gtagcttttg gcataagttt
    18541 tactaagcta cagatgaatc aggccggagc ccttgtgcat atctacagcg atggctcggt
    18601 agtggtctcg acgggcgcca tcgaaatggg ccagcaggtg gcgcgaaaaa tcgcgataat
    18661 tgttgcaaag actctcggtg ttccaataga tcgagttacg gttcaacgca ctacaacttt
    18721 gacagtagcc aatactgtgc cgaccgccgc gagtacggga tcggatatga atggtatggc
    18781 ggcaaaaatt gcatgccagg aaattcgagg aagactctta aagaaagccg cagagctcct
    18841 tatggtcgat ctcgaaaaga tcgatatcaa agatggagca gtgctagtag atggagcacc
    18901 tgatcatctc agctgggagc agcttgtcga ggccatgagc gcttcacggc aagatctatc
    18961 cgcacatgga ttctatgcga cgccgatgct cgaatatgat atggaaacag aacgtggaaa
    19021 gccttttgcc tatcatgtct atggcgcagc gctggtagaa gccagcatag atgtcctacg
    19081 aggcacatat acaatcgaca gggcaacaat tgtccacgat attggtgaga gtatcgatga
    19141 aagcgtcgat atagggcaga tcgaaggagc ccttgcacaa ggcctaggct gggctctgct
    19201 cgaagatctg cgcttcgagc ccgatggtaa gcctatttcg gacaccctat ccacctataa
    19261 agtgcccgat ataaacttta tgcctcccaa tatagatatc gaattcctgc caagcatcga
    19321 taatccattt gcaccttata actcaaaggc agttggcgag cctccattgc agtatggtat
    19381 tgcgggctat tttgctgttc tcgaggccat tagagctgca acaggaaaga cgctcgaata
    19441 ttacgatctg ccccttatcc cagagaaaat aatgaaactg ctcatttcag agcagaacaa
    19501 aatagataaa gtaactggaa atcacattgg gacaaaagcc tgatgcggaa tattcttggc
    19561 agggagaatc agaatgatat tagcaaatgt cgctgcagca ttgcctggtg agcgcgattt
    19621 taggcttgtc aatattttca taaaaggcaa taaaatagct tctatcaagg aagcagacct
    19681 taagaagaaa acagataatc cgttggaaaa tataaattcc ggggcgaatg aagcaactga
    19741 ccttcatgct tatgatgatg aagaaatcat agatgcacat gggcttctcg catttccagg
    19801 tgcaatagat ccccatgttc acttcgatga gcctggcttt acgcatcgcg aagattttct
    19861 gcatggctcc gcagaggcag cacgaggagg cgtgactacc atcatagaca tgccctgtac
    19921 ttccattcca cctgttatct ctaacgaggc actcgaagag aagctatcaa tagtacaaaa
    19981 aaaagcgttg atcgattttg gcttttatgg tggcataaat gggaatatga ctcccgaaca
    20041 tattcctgaa cttatagcat cgcttgctcc tcgagtgctt ggcttcaaat cctatttcat
    20101 ctcaggcatg gacacattcc cagcggtaga cgaggcccaa tttgccgccg ctgcaaaggc
    20161 ttgcggaaaa gaggggcgcc cccttctttt acatgccgag gacgcttatg taatttcgga
    20221 agcaaccaag gaaagagaat tgctacgtgg cgcacaaaaa ccgcaatgga tagactacta
    20281 cgcctcgcgt ccaatggaag ctgaaatcgt agcggtgatg aaggctctaa aacttgcagg
    20341 acaatatgcc aaatatgtac acattgtaca tattggtacc gccaaggccg ccatggcagc
    20401 ttttgagcgc ggcgccacat gtgagacctg ccctcattac cttgcgttcg atgagacaga
    20461 ctttgctcgc tttggagctg cactcaagac ggctccacca gtgaaaagcc cagaacaaaa
    20521 atcaattctt tggcaccttc tcgcagatgg taagataaat tttgtcacat ctgatcatgc
    20581 gggagcacca gattatgaaa aattcacaga caatccattg atggcgtatg gtggcattcc
    20641 tggtactggt acactctttc cctatatgct atctgaggga ttgtttgcca agagactctc
    20701 tctcgaacgt tttctcgagg taactagtgg aggcgcggca agaaggtatg gactttggca
    20761 aggaaagggc tcgcttgccc ctggcaaaga tgctgatatt gtccttgtcg atccagagca
    20821 tacgaggcgg ctcaatccct cggctatgat gagcaaaagt gcgataacgc catttgcggg
    20881 aatggtgctt tcgggacgca tcgaaggcac atgggtgagg ggttctatcg tatatgcatc
    20941 ggttaggctt gctgcagcgc tcggagcacc caaagatcct atattttcca atgaagaagg
    21001 cataattctt gccaaacctg gttatggcac atttcttact tgggggtatc gatgagcaag
    21061 ataatgaagg ccacagcgtt gcccgccctg tttgcgcgca ttgcaggcga atacaaagca
    21121 aaacgtagca ttttcgaaat tccggaatct acttggctag atatattcga acaggaagcg
    21181 gagagcgcag gcatgcctgt aatggcttct acaataagca ttccactagg gcctgcagcc
    21241 gggcctcaca cgcagattgc tcctaacctc atcgcagcat acctttcggg agcgagggtg
    21301 ttcgaattaa aaacggtaca ggagaacgat catctcgaca tcgacaagcc ttgcatcgat
    21361 gcgctcgacg agggatacaa tgtcgaatgg tccaccgagc tctcgctcga agaggcacgt
    21421 attgaatata taaatgcctg gatggtcatc aatctctttg caagattatg gtcgcataaa
    21481 ccaagcgatt ttttattcaa tatttcggtg ggttacacgc tagaaggcct caagagcgaa
    21541 aaaatggacg cttttatcga aggtatgcgc agacctgaaa caggctctta ttgggaacct
    21601 gcgcttgaag agctaaagtc tttcgtcgaa tctccacttt ttatccaggc ttttggatct
    21661 caagcgctcg aaaaagcccg ttccatagca gatcacatgc cggttcgacc tgtacattcc
    21721 gtaacactct cgaccatgca tggctgccct cctgaagaaa ttgagcgcat aggccgctat
    21781 ctcattgaag agaaaggctt tgatacctat ataaagctaa acccaacact tctcggctac
    21841 gaaaatgcac gaacgattct cgataggctt ggatggaagg atattgtcct aaaacgcgag
    21901 agtttcgagc acgatctgca attcgacatg gctatatcgc tcataaacac cttaggcgag
    21961 gttgcggcca acaggggaag gcggtttggc atcaagcttt ctaatactct ggccaacata
    22021 aataacggtg attatttacc tggtatggaa cgatacctct caggccgggc tcttttccca
    22081 ataacaatcc accttacagc aagactagct catgctttgc ctgatttcgg agcgcggttt
    22141 tcttattgcg gcggtgtctc agccttcaat gcggccgatc taatcagagc gggaataggc
    22201 ccacttacaa ttgcaaccga tttactaaag ccaggcggtt atcagcgctt aggacacatg
    22261 gtcaaagatg tactctcaag tctaccattt gcgcccgaga gtccagattc cgcagcttta
    22321 gatgcacttg ccgaaggagt atttttaaaa ccctattacc gcaaggaatg gaaagaaggc
    22381 atggcttcca ttgaaggtcc tttgccgctt ttcgattgtt ttgcagcacc ctgtattgaa
    22441 gcatgtcctg ttgtacaaaa ggtacctgct tatgttgctg cgacaggaac tggtgatgca
    22501 aatagagggc tttccattgt tatttcggat aatccgctgc ccacaataac tggaattctg
    22561 tgcgatcata tttgccagga gcattgctca cgcgttgatt atgagggcgc ggtacgaatc
    22621 cgagatatga aattatcgac cgtgcgagca gcaaactcag aatcagaaaa tgaaatgcga
    22681 ccagcatggc ctagagaagc gattggaaaa acagcgatag ttggagcagg gcccgctggg
    22741 cttgcttgtg cttggtattt ggcccaacat gggcagaaag ccgttatttt tgaaaagtta
    22801 cctattgctg gcggtgtacc ttctacggtc attccttctt tcagaatttc tcaagaggat
    22861 attgcatcgg atatcgatag atttgaaaaa ctaggtgtag agtttcgatt cggtactgag
    22921 atttctacta tagaagcttg caaagcccta gaaagagatg aattcgattc cattgtcatt
    22981 gctcatggag cataccatgc tcgcaattta atgcttggtg gcgatggcgt aaaagtcttg
    23041 catgcactcg attttcttcg ctcttgcatg gaaaaaggtc ttgatccttt tagtggagcg
    23101 aaaaatatac ttgttgtggg aggaggcaac actgcatgcg acgctgttag gttggcatct
    23161 agaatccctg gtgtagaaca tgtctactgg tcttatagac gcagcaaaaa cgagatgcct
    23221 gcagataagg aagagctagc aaatgcaatc gcggaagcga ccgccctaat gattagtcct
    23281 gataatcgag cttcatattt ttctcaagat ccctcatctt ccccagattc atcttcagaa
    23341 gagaaaaatc aagaatctat ttttcttgaa ttgacgcttc ctgaagcgct aggaccaggc
    23401 acaataacgt tgcgtcgcat gaaactaggc gagaaagatg agtctggacg gcctagtcca
    23461 gtaccaactg atgaaacatt ttcgcttcct tgcgacctca tgattgcagc agtgggcgaa
    23521 gcgcctgatc ctacgctctt caagaatttc ggaatcgaag tggcaaaaaa tggtcttcca
    23581 atcgtagacg aagagacaat ggaatcttcg attcccggaa tctatatttg tggcgatgct
    23641 cgtagaggac cttcttcgat catagcctcg gaagcggatg gaagagcggc agccttagcc
    23701 attcttaaaa aaagagaaat agaagtaatc aataaagact atcgagctcc tttatgggac
    23761 agcacatctc gtctaaatcg tggcaagatg atggaatctc tatctatcga tgacccctca
    23821 tttgcagcaa tagaagctga gcgttgtctg gcatgtgatt cggcttgtct gcgatgtgtc
    23881 gaagtctgtc ccaaccgagc gaatataatc atagaaacag gaagactgtt cgatcaacct
    23941 gcgcaaattc tgcatatcga tcgcctctgc aatgagtgcg gtaactgtgg gcgattttgt
    24001 ctatggaaag gtgagcccta tagggataag ccgaccctct ttgacaccat aggcgatctc
    24061 catgtctcga ataatgcagg ttttgctttt tctggaaata gggaaagacc atcgctggtt
    24121 ttccgagcaa aagcaggaga atctgtcgct gaactaccat ttttcgcctg gaatggagtt
    24181 atttctctat cggtgcatcg cgatatcctg gccttagctc gcgtcgtttg gtctgagcat
    24241 agatatctcg tggaggtgca tgaatgattt tgtttcgcaa tgtaagaatt atggaactgg
    24301 aaccgccttc ggtttccgaa ccgatagatg tagcggtata tgagccagac gaaggcgcac
    24361 atggcggtac tattgccgag ataggaacct ctttgcagac aaaataccca aaagccaaac
    24421 tagcgggaga tggaggattc ctttctccag ggcttgtatg cggacatact catctatact
    24481 cagtgctttc gcgcggcatg ctcgtagata ttgcgccatc caaggatttc gcgcagcttc
    24541 tcgaacatct gtggtggcgc ctcgatagag caatcgaccc tgagattctg caagctagcg
    24601 ccctagcagg ttgttccgac gcgctcaagg caggagtcac gagccttgtc gaccatcatg
    24661 ctgggcctga atgcattgct gatagtcttt caattatacg aaaagcctat gaagatacgg
    24721 gtttgcgcgg gattctgtgc tacgaaacaa ctgaccgcaa tggaatggct gaagcacgag
    24781 ctggaatcgc ggaaaatgta agattcgcca aggaggtgga caacatccgg gcatcgggtc
    24841 ttaagcctct tgtcgaagct gctatcggcg cacatgctag ctttactctc agcaatgaga
    24901 ctctggaagg acttgctgaa gcagtacagg agacttgccg aggcattcat atacatctcg
    24961 cagaagacaa gttcgatgct gtcgatgcta gatatcgctt tggcaaggat ccggtagagc
    25021 gcattgatgc tgtaggagct cttaaccctc gcgggcttat cggccatggc gtatggctca
    25081 ccccttccga agtagagata atgaatgcta gggatgcctt ccttgcccac aatgctcgtt
    25141 ccaatatgaa caatgccgtc ggctataaca ctctattgcc gacattcaag aatgttgtgt
    25201 tgggaacgga tggcatgggt gcagatatgc tcgaggaatt caaattcgct gttttcaggc
    25261 accgcgaaag tcaaggccca tggtggcctg gtgactttct taaggctttg cacagaggca
    25321 atctccttat agagcgatat ttctcacagg actttgagcc aacatttcct ggattcggta
    25381 ctataaagcg aggtgctccg gcagacctcg tgctctggga ttatgatccc cctactccgc
    25441 ttgtgggagc aaacattgct ggacactttg cattcggaat gtccagtaga tcggctcgca
    25501 cggtaatggt tgcggggtcg attcggattc tcgatcatag accattgtat gatgacatag
    25561 ctatcgaagc agaagcatgt caccaagcct caaggctctg gaaaagaatg gaagagaggt
    25621 aataaatgga aacaaccaag attctagaaa aaactcatga ttatcgcgat tatactgccc
    25681 agaatctgag caaaatcatt aaggtgccag cgttttccac cacagaaaaa gaacgaattc
    25741 agcttttgaa gaagctatgc gaagaagctg gtatggagga tctgtggatc gacgggcttg
    25801 gatctttgct cggccgagtg ggaaaaggct cgaaaaagct tgtattcgat gcacatatcg
    25861 acacagttgg tgtcggtgat gaatcacaat ggaaaacccc tcccttttct ggattgatca
    25921 agaatggcct tgtatatggg agaggagctt ccgatcagct tggcggagct gcctcaatga
    25981 tcacagctgc gcatatttta aaagagttgt cttatgaggg agactatgaa atctggtttt
    26041 cctttactac gatcgaagaa gactgcgatg gcatgtgctg gaaatacctc attgaagaag
    26101 aaaaattcgt cccagatttc gtagtatcga ccgagccaac ttcctgcagg ctctatcgag
    26161 gccaccgtgg acgcatggaa atccaaatcg atataaaagg aatctcttgc catggctcgg
    26221 ctccggagcg cggcgatagt gccgcataca aagcagctcg agctgccctg gcgatcgaaa
    26281 aactcaatac ccaacttttg cccgacgatg atcgtttcct tgggaaaggc acaataaccg
    26341 taagccagat agatgtgcac ggtccaagcc agtgcgccgt acccgatcaa gctatgctat
    26401 attgtgatcg tcggctaacc tggggagaaa ccgacaaaag cgctatcgcg caagtacagg
    26461 ctgttctaaa agaggcgggc gtagatgact ttactgtcta tatgcctgaa tacagaaagc
    26521 cagcctggac cggaacggaa tatcaccaag aactctattt cccaacctgg aagatcccag
    26581 aggatcacat cctcgtgcaa tctggcgtcg aagcatatac agcgcttttt ggagaaaagc
    26641 ctatcgtaga caaatggacc ttctcgacaa acgccgttgc gatctgtggc cgccataata
    26701 tcccagtgat cggattcgga ccaggagacg aagctcaagc ccatgcaccc aatgagatta
    26761 cgcgtgtaga cgatcttgag atagctgcgg ccttctatgt cgccctcccc tttgcgctat
    26821 cggctttgcc aactcgagaa agataaatta tcgaggcata aaagctatga aatggttcta
    26881 tcgctgtcct ttatgtggaa aaacatatcc aatcgaacct gggcgctatc tatgcgacag
    26941 ctgtgcggaa gctcagaaac caaatgaacc cttgcgtgga atactagaat gctcttggga
    27001 aggagagaat tcatccagag gttctattcc cctccccgtg gaagaatcct tctttcctcc
    27061 aattcctgtg ggtcaaacgc cattatgggc tcccgagcga ttacggactg agcttggaat
    27121 accacatcta tatctcaagg atgatacctg taatccctct ggctcgtata aggaccgagc
    27181 aagctggctt gtagcagcct ttgcgcgtaa atttggcata aaggaaattg tccttgcttc
    27241 gactggaaat gccgcatcga gcatggcctg cattggagcc gcatctggca tagcgataaa
    27301 agtctatgtt ccaaagtcgg ctcctatcgc aaagcgagtc cagattctgc agtatggcgc
    27361 agagcttatc gaggtcgatg gcacatatga cctcgctttc gataaatcgc tcgaatactc
    27421 caagtcgacc ggtatgcttt cgcgcaacac cgcatacaat ccactgacga tagagggcaa
    27481 gaaaaccgca agcttcgaaa tagccaagga tctggcaaga gctgaagata atgtaaaaaa
    27541 acatggtata aacacgaatg taagccaaga tgaagcgctt aaagttccca atattcctgc
    27601 ctatcttgcg cctgaccaca tctttgtacc cacaggggat ggcgtaattc tctcgggggt
    27661 gatccgaggt ttcgaagatc tcctgagatt gggttggata gatagaattc cgactgtatg
    27721 ggcagctcag gccgaaggct cgtgtgcaat agccagagca ctggcaacag gtgttttcga
    27781 agcgcgtcca tcgcgaacta ttgccgattc gatagcggtc gatttgccga ggaatggtta
    27841 ttttactctg cataagctta aaaagcatgg cggtcgcgcc gttgtcgttt cggacgagga
    27901 aatccttaag gctcaaactt atgttgcaag gctctccggc cttttcgccg agccttcgag
    27961 tgcctgcgcc tttgcgggat ttatgaaggt gcgctcagag ctcgatcccg aagcgcgtgt
    28021 tgtggttatg ctgacaggca acgggcttaa agatatcaaa tcagctgcca tggccgttgg
    28081 cttgcaaaca taatcgtctt atcgaaaaat caaaatgcat gcataaatat catggtgaat
    28141 agaaatcaat cgagtgccaa acctttttcc aaaaaaacct tgatgcaggc atccacaaca
    28201 tcggaatcat ataggatccc cttgttttcc ttaatctcat cgagcgctgc atccaatcca
    28261 agtgcttctc tataggggcg gtgataagtc atggcttcga cgacatcggc aacgcctatg
    28321 attctgcttg caaacaagat ctcatcccct ttgagcccta acggatagcc tgatccattt
    28381 atcctctcat ggtgttggag tacaatctcg gcaataggcc atgggaaatc tatctttctt
    28441 agtatttcat aacccgtttg aggatgttcc ttaatgagct cgaactctgc acttgaaaga
    28501 tgagctggtc tagaaaggat gtcggcaggc acacgaactt tgccaatatc atgtatcagc
    28561 gaagctaccc atatgccttg ggcagcatct tctgacaagc caagctgctg ggcaatagct
    28621 ttggcaatcc ttgcaacccg gttctggtgc cctgccgtat acggatctcg catttctacc
    28681 aaggcagaaa gcgcattaac ggtctcctct aatgtttttt gcagattccg atagctctta
    28741 tccagctctt gttctgccat ttttcgattt gtgatattat gagccgccat tatggcatgc
    28801 gtaacgactc catcgctgtt cctataaggg atatagttga tttcgaaaca ttgagtgccc
    28861 attccttcca tatcgaacca gtcttcggta ttgatatctt ctccattttg acagcgttcg
    28921 aggcaaggct tgattttttg ctcatagact tcttggcccc aaatttcact tactgaatgg
    28981 cctacaattt cttgctgcct ttctttgcca aaaatcttgc aaaagctgct attgacagtt
    29041 tcaaatattc cagatctatt tatcaaagcc atcatatcgc gcgaagcatt gacgataaaa
    29101 tcggattttc tataggaatc ctcggcgatt tttcgggatt ttctatcttc agattccttg
    29161 agctcgcgct caattgcggg aacaaggcgc ttgagattgt ctttcattac atagtcatgc
    29221 gcgccttcct tcatgagttg aaccgctttt tcttccccaa cagctccgga tacaataata
    29281 aaaggtattt ctattccgca ttctctataa acagataggg cttctgcagc gcagaattct
    29341 ggcatgttat aatccgaaag gataatatcc catttttgag atgagagggc tgatctcatt
    29401 gtgtattcgg tttccacacg ctcagataat ggtgtataac cagcttttcg caattcgcgt
    29461 aggatcagca cagcatcgtc ttcggaatcc tcgattaaca gcactcgcaa tggtttatcc
    29521 attcggttct ccattcgcta attagcctaa taaagtattc tattcattac tgcaatgtaa
    29581 aatagaaggt agcgccttta tcgacttcgc cttcggccca gatttcccct ccatggcggt
    29641 gaattatgcg ctttaccgaa gccagcccta tgcctgttcc gctaaaggcc atcgcattat
    29701 cgaggcgctg gaatggatcg aaaatcttat ctgcatattt catatcaaac ccagtcccat
    29761 tgtcgcgaac ataatagatc gtcttagaat tcttctgcat gacgccaaat tcaattcttg
    29821 cgccttcacg ttcttgcgtg aatttccatg cattgccgat caggttctct aaagccatcc
    29881 ttagaagcat cggatctcca gctgcaacga tgccttctgt gatcgagaat ttcaccttac
    29941 gatcgggata ggttgtttca agttctaggg ctatatcctg cgctaacgcg ctaagtttca
    30001 ctttttctct gtgcatctca tatcttccaa tgcgagagag ctgaagaatg gcatctatca
    30061 tggctgtcat tcgctgacat gccgccctta tacgatgaag ataatcctgt gcctctcgat
    30121 caagccgatc ctcgtaatct tcttgcagag cttggctgaa accatcaatg ctcctaagcg
    30181 gcgcacgcag atcatgggag acggaatatg tgaatctctc tagttcctgg tttacttgct
    30241 ctagctcctt acgaagcttc gtttctgtca tatcgacgac agtgatgagt gtcgctcttt
    30301 ccccattgta ttcgatcgcc gtgttgctaa tgaaggcagg cataaaactg ccgtcctttc
    30361 tgaccattcg catctcatac gtctcgataa cttcttttcc actatatcta ttgtaggctc
    30421 tagacatgat aagttctctt tcgtctggat ggattatttg taagatgggc aggcctatca
    30481 tttcttcttc ggagaatctt aacatggatg caaactgcct atttgcaaag accatttggg
    30541 cgttctgatg aatgccaata ccaaccaatg catgatccac aagtgcttta tatttttctt
    30601 ctgattcctt gagttctttt gtccgggcct taaccatttc ttctagagat ccctggtatc
    30661 gcctcagttc ctcctccatt ttttttcgtt ccgtgatatc ccgaaatatg agtatggtgc
    30721 ctgtttttgc accaaagcga tccagaatcg gcgaagaagc aacattgaaa tgtcgctcag
    30781 agtccttgaa agcaatgtct tcatcagcga tctttgcggg cgtgagcaac aagtttttta
    30841 tcatagcttc cactttttca ggatcctctg ttccgctcga aactagagaa actatactaa
    30901 gaaaatcttt accgagaaga tcgtgcctct ttatacccag catggtctcc atggatggat
    30961 ttgcttcttc tattttgcca tttttatcta taacaatgat cccatcttcg attgcttcga
    31021 aaatagtacc cagagaagcc cttttatatt ttattattgc ataggcctga agcgatataa
    31081 aaacgaggac tagcagaaaa ccaatcggat agaaatgaag cgatggaatg gaggaaagaa
    31141 agtcgacgga agccaatgca gctatcgata aggatatcat aaacaacctt atctggtcac
    31201 gctttgcccc aggctccatt tgtctaatgg ctcttcgatc gagctgcagc gaccgaatta
    31261 tgagaacaga aaagaaaagc aggaaaggta ttactaatgg accaaatact atgtcatagc
    31321 cataaaagcg ttcttccaca gaagcgataa gccaatctgt cagcaaacca aagatataga
    31381 aaactagagc gatggcataa tttgcaagaa tatagcgctt cttttcgttg tatagatcca
    31441 gagatgttat ggtaaaaaag tatattccag gggcaatcat gattattcct aaaaaagtga
    31501 atactttgca aaaagagagt accagtgcag aattcttcat tgaatagatt aaaccagtgc
    31561 cacattgcca aatactggca ctagctgtcg taacaaaaaa ggattggctt acaatattct
    31621 tatcctgcaa aaggacatag agcccaagca ataaaaccaa gagagaagta ataaacatag
    31681 gaatggaata tggattcaag aaatagtttg cactattcag aaactgactc agcatttatc
    31741 ccccttactc aatcgagcta tgcccaattc agagagatgc cttactaatt tatattctgc
    31801 tgcattcaag aggcattttc aagactttgg actttaaaaa gatttatggc ttacattgct
    31861 cgctatacat gaggcaaaca gataaacaga taaaccctcg cagaaaccag gttagcatgt
    31921 tataatgcat atattttgat catttataag cgttatcaca gcattgcaag gagtctagca
    31981 tgatcgatct agccatcgac gaatcccaaa gaaaaaagaa tatcgaacat tgcaagaaaa
    32041 gaggaattct tctgccaact tttgcccaga tgtgcgatcc cgcaaagata ccaagtgcaa
    32101 taagggctga actctacggt attggccttt gggatgtaca tccgcggaat ctattcagag
    32161 tcaactggca caatgagccc aaggaatttg gcggtggata tggatcagtc aatttcatcg
    32221 aaattcctcg atctatctct ggcacaaaag cccgaattat cggactcgca ggcaaatggt
    32281 tccctaccgg tgcacataaa gtaggcgcag cttacgcctg ccttgctcca gagctcgtaa
    32341 ctggcaggtt cgacccaacc agcaagaagg ctgtttggcc cagcacaggc aattattgca
    32401 gaggcggagc atacatttcg cgcttgctcg cctgcccgag cgtcgcaatt ctccccgaag
    32461 gaatgagccg cgagcgattc gattggctca agactatggc cgaagaaatc atagccacgc
    32521 caggaagcga atctaatgta aaagaaatct tcgataagtg cgtcgagctg caaaaaactc
    32581 gccaagatgt agtcatcttc aatcaattcg atcagctgcc taaccatcta tggcactatg
    32641 cgatcaccgg cccagccatg gaagaagtgt tcgctacgat cagcggcgct gctacccaag
    32701 ctggtgaaaa gggcacccat gctaacggag aaagcgctca ggtttgcggt gtagtgctat
    32761 cctccggctc agctggaacg ctcggctcag gctcctatat caaggagaaa ttccctggcg
    32821 caaagctcgc ggtcgcagaa gcactgcagt gccccacaat tctagagaat ggttttgggg
    32881 ctcatcgtat agaaggaatt ggcgacaagc atattccatg gatacacaac cttcgggata
    32941 ccgacgctgc ggtcggagtc gatgatgaac ttccaatgcg cctcattcgg cttttcaacg
    33001 aaccggcagg ccgagagctt ctattggaaa caggcaccga tccttctata gtggaaaaac
    33061 tggattggct tggcatatcc ggagcgggaa atctcatcgc tgccataaaa ttcgcaaagt
    33121 actatgaact tggcgaagat gatatcgtat tcacaatgtt caccgactcg atggaactgt
    33181 atcaatcgcg tctcgtggaa ctaaccgcag agcgcggccc atacgatcag cgccaggccg
    33241 accgcgacta tgaatgcctg caggacttat cggtcgatta cgttttcgaa atgacgcatg
    33301 tcgataagcg gcgtgttcac aatctaaaat attttacatg gatagaacag ctcggaaagg
    33361 agctcccaga acttcgagct cagtggagcg attatcgcaa ctattgggga agcttacacc
    33421 agcaagttgc ggccttagat gagctcatcg aggatttcga tgcggaggtt caaaagtgat
    33481 cctcttaaag gattgccttc gagtagttcg ccttgaagat gaagatctaa acggcgtcga
    33541 cgtgctcctg gaaggaaatc ggataaccca aattgcaaaa aatatctcaa ttccttgcgg
    33601 cgctcgggtc atcgatgctt cgcatcttgt tgtagcacca ggcttagtca atacgcacca
    33661 tcatttctat cagaccctta cccgtaatct accagccgtg cagactgcca agctcttcga
    33721 ttggctcgtc tatctctatg aaatatggaa aggtatcgac gaagaggcag tctatatctc
    33781 gagccttttg gccatggcag aattgataaa gaccggctgc acgaccacta cggatcacca
    33841 ttacgtatat ccacaaggtt ttgaggccga tatccccaca cttcagttta aagccgcatc
    33901 agatatcggt atacgctttc ttgccacacg tggctctatg tcccgatcga agaaggatgg
    33961 tgggcttcct ccagattcgg tagtgcaaga tgaagaaagc atattagctc actgtgagga
    34021 tacgattagg aaattccacg atccttctgc ggactcgatg catcgcgttg ctttagcgcc
    34081 atgcagtcct ttttctgttt ccgaggagct catgaaagag tcggcaatcc tagctaggaa
    34141 atatggagtg aggcttcata ctcacctagc ggaaacagcc gacgaagagg actattgtgt
    34201 tcagatctat ggccgccggc ctttaagact catggaagac tgcggctttc ttggagcaga
    34261 tgtttggtat gcccatggca tcttcttcaa tgataaagaa atagagctct tagccaaaac
    34321 caggacaggc attgcgcatt gtccctcttc caacatgaga ctgggctccg gcatctgccg
    34381 cgtaagggag atgctggaca ttggagtccc ggtggggctg gcggtcgatg gctctgcttc
    34441 caacgatagt tcggacatgc ttggagaggc aagacaagcg ctgctactgc agcgcgtacg
    34501 ctatggcgcc actggcctag cttccaagga agcctggcga attgcaacaa aagggggtgc
    34561 cacaatcctg ggttgggaac aagctggctc tatcgaagaa ggagccttgg cagacatagc
    34621 ctgctggaat gtcgataagc tcgcttatgc aggatcgctt tcggacccct cggctgctct
    34681 cctgttctgc ggctacgatc atgccgccga gatggtcatt gtcaacggaa aaatagtggt
    34741 cgacaatggc cgcatcgtct gtatcgacga agagaagctt aaagaagatg cgaacaaggt
    34801 cgcccttcgc ctttataaga aggcgggaat aacataagga tcaaggagga ccttatgaac
    34861 acgactgctc tcattattgt cttggtttac ctggctgcaa tgcttgcaat agggttctgg
    34921 acgaacaaaa agctgatcaa gacaagcgcc gattacatgt tggcaggacg agccgtgccg
    34981 atgatcatcg ttgcctgcag ccttgcagca aacaacatag gtggcggttc aactaatggc
    35041 cttgttaacc gagcctttgg ttcttgggga atttcggcgg tatggtatgt gatggctgcc
    35101 tctattggcc taattccaat gattttcttt gctcctaaac tacgaggcgt aatggctttt
    35161 actataccgg aagttgtggg gcgccggttt ggcaagacag cccacatcac ttcggccatt
    35221 ctcaatatcg tatcgctatt ctgccttacg gcatctcaaa tccttacatc gggcaccatt
    35281 ctctctcttc taatcgggct agatctcaat tttgcaattg tactcgctgc gggttttacg
    35341 attttatata ccacaatggg agggctctgg gcagattcta tcaccgatct tttccaatgg
    35401 atcattattt tccttggact gctcatcgca cttccctttg ctgtgaacgc agcgggcgga
    35461 tggaacaaca tgatgtcaag cctgccttcg gaaaagctta aaccatttgg gacgctgggt
    35521 tttcttggaa tactctctct ggtaatcaat tactttctta cctttaccgc tggccctgag
    35581 atggtctcaa gaattttttc tgctaaggat gcaaaggcta gcaaaaatgc tctaatctgg
    35641 gcagctgttt ttatgggtgt cttctctttt gtacctacat tcattgggct agctggctat
    35701 gcgatcaatc ctaacatgcc aagcaatcag gttctggcaa atgtcatctt cggccatact
    35761 cctgcctggg tagcgggcgc ggtctcggcc gcaatcatag cctctacaat gtcgagtgcg
    35821 gattccgata tgctctgcgc ctcgacgatc attaccaagg atttgcttcc ttacttcaag
    35881 agcgatcttt cggataagtc tcagattgcc atttctcgcg cgatgaatgt gataataggc
    35941 attggagcca tgttcattgc acttttcaag atcgatatca tcactctcaa tgtcttttct
    36001 tttatgcttc gcgcggctgg acctattgca gcctttttac ttggtcttct ttggaagaat
    36061 gtgggtaagg ctgcaggcgt catatcgatt tttgtcggtt ctgcggtagg tatcggttgg
    36121 caaatccttg ctaatcagat gaaggtggat aatccttatg gatttctacc catagtcgta
    36181 ggttccgctt ccagctttat aacatttatt attgtaactt acatagaacg ggctatgggt
    36241 cggccacctg ctccggaatt aaaaatcgaa gaataatgcc tcaatcttag aatgaggttt
    36301 ttcttgaagt agcttttttc ttgaaaggaa attttttctt aaaaaggaga ttttttgaat
    36361 ggatagaaat caaatcgaga acctcataga tgatcttcgc ggcaagaaaa ttgcaatgtg
    36421 taaaaaggat ttcttgctaa catgggagaa aacagtccca gaaatcgagg ccaccttttt
    36481 agttgccgat atccttcgcg ccatgagaga tagcaacatc tcacctaaag tctgggattc
    36541 aggcatcgca gtatccaatt tccgcgacaa ttcgacccga actcgctttt cctttgcttc
    36601 cgcttgcgat ttgctgggtc tatacatcca ggacttagat gagggaaaga gccaggtagc
    36661 tcatggcgaa accgtccgcg agactgcaaa tatgataagc tttcttaccg aagccatagg
    36721 aattagggat gatatgtatt taggcgaagg cgatagatac cagcgagaag tgggaaaagc
    36781 tctagatgag ggagtaaagg caggcgtttt gccttctcgg cctactatag taaatcttca
    36841 aagcgacatc gatcatccaa cccaagctat ggctgatttt ctgcatcttg tgcattactt
    36901 tggaggcctc gataagctta aaggcaaaaa gatcgctatg agctgggctt atagcccctc
    36961 ttacggcaag ccactttcgg tgccacaggg gatcatagga ctagcgagcc gcttcggcat
    37021 gaacattgtg ctcgcatatc caaaaggata tgacctcatc ggtgatgtcg tcgaaatgtc
    37081 caagaaacag gcggccgaat ctggcggaag cttttcttac actgattcga tggacaaagc
    37141 cttcaaagat gccgatatcg tgtatccgaa aagctgggcg ccctacgatg taatgctccg
    37201 ccgtgtgccc cttctccatg ctggagataa agaaggcttg aaagaactcg agagagaagc
    37261 gcttgccaac aatgcaaaat tcaaagactg ggaatgcacc gaggaaaaaa tgaagctgac
    37321 gaggagcgca ggcaagaatt ccccacaagc actctatatg cattgcctcc ctgccgatat
    37381 caccggcgta tcttgtgcag ccggcgaggt tgccgccagc gtgttcgatc gctaccgcga
    37441 tgacacatat agagaggcca gctacaaacc ctatatcatt gcggcgatga ttctcttggg
    37501 aaaatttgaa cgaccagccg agctgctcga gagcctctta gccagaggca gacagagaac
    37561 ttacggcaac ttctgatatt cggtcttttg taaccatgtg gattctcttt ttggctattg
    37621 gtttagcagc tttctagaaa gcgctgggca attgttttat agaaaatgaa atgccatagg
    37681 cgcggccaag tatgtcttcg ttgacgaggc cacccgccgc gccgatccac tggatttttc
    37741 cttctgagat gataagtgca tgacctccta gaaaaatggc taggtcgata tcgtgcgttg
    37801 tgtagattat cgtcttgcct gaatcggcaa gggaagcaag cgcatcagcg atatgccgcg
    37861 aatgcgctgg atctagcatg cttgccggct catccagaat tagaatagaa gccccttgag
    37921 caaggcaacg cgcgattcgc acaagctgaa actcgccacc ggacagctcg ccgatattgc
    37981 gcatgctaaa cgaatctaac ccaaaaagag aaagcgcctt tagtgcggct tcttcatctt
    38041 cctttcctgg caaggccagg acttccatgc aaggcgtgcg acccatcaat acaaaatcca
    38101 agactcgata attgaaggca agcttttcaa gctggggcaa aaatgctatc aaccgcgagc
    38161 gttctctggc cgaataggaa ataagcgggc gactatcgag attgacggag ccggcgcgcg
    38221 gactgaggcg accagagaga atggagagca gggttgtttt tccagcgccg ttggggccga
    38281 ggatggcaag cgtttggccg cgcgccacat cgagcgagat atcctctagt agtggctgat
    38341 cttctttcca tctaaaagag atatcgcgcg cagaaagaac cgattcgctg ttcatcgtgc
    38401 gagctccact tttctagtgg taagcaggat agcaaagctc agcgcaccca aaagagaggt
    38461 tataatgcct agcggaatct cacctgaaaa gacagatcgc gcaatagtat cggaaataag
    38521 gacaaaaata gctcctccga gcatggagcg cggcaccaca aaacgtccat ctggaccatc
    38581 tacagcgcgt acaatttgag gaatgataag cccgacccat ccaatcactc cacacactgc
    38641 cgtcattaca cctacgcccg ccgcagccgc cacgctgact attctacgct ctagagatgg
    38701 ccgcacgcca agcgaatgcg ctaccgcatc atcgagcgat agcaaattga ccctccaacg
    38761 catagcataa aggactataa gagacagaac tgcaattggc gctgcggatt ctagcctctt
    38821 ccaactcatc ggtaccatcg atcccatggt ccaaaagact atatcgggca actgatgcaa
    38881 aggatctgca atgtatttca tcaaggataa aagcgatgaa aaaaaggctg atacggctag
    38941 cccagcgagc acaagcctca aaatctgccc gccatatctg aatcgctcgg agagccatat
    39001 cgaaagagca agcgctaaca ctgccatgcc aaaagccagc gcagcgatga ccgaataggc
    39061 acgaaacccc aaaaccagcg cgagcgcagc tccgaaagct gcaccttgag acacacccaa
    39121 aaaaccagca tcgattagag gattgctaaa aatagactga agtgaagccc ctgctcctcc
    39181 aagcacaaga ccaagcaata tcgcccctag aatgcgtggc aggcgagaat ttagtacgat
    39241 ctggcgcgca attggatccg ttttgagcaa acaaggattc attatgccag gtactggata
    39301 gcgaccaata aaaagcgaaa gtactgtgac tatgataagc gcaatgccga gataaatgac
    39361 cagcgaagaa tggtgcggac tattgggaga agagcgaccc tgtttcacac tagaattgct
    39421 cccctatatc acctttgatc ttgggctcga tgatagagcg aaactttgct tcatcgaatc
    39481 cgtacatgaa gctatagaaa tcccgagttg tctgcactat cgagatatct ctaaagagcg
    39541 ctggttgaat ctgctttgca agccatgtca atcccagaat ccatcgcgta tctggctgat
    39601 cccaggaata aaaatcctgt gggaaaccat atacctttcc ttccttcacc gccttgagcg
    39661 aggaaagccg cttatctttt ttgaaagcct cggccgcttc cgatgaattt ccacgataat
    39721 tgatgataca cactacatca ggattccagg ctgcgatctg ttcaaccgat accgtagccc
    39781 atccagagcc aggattcgca cctttccata caggaattcc acccgcgcgc tccgctatga
    39841 ttgtctgcat ccacgaatca ggcggaacct gccagaccga ttctcccgaa gccgctaact
    39901 gcattaggag gacctttggt ttttgagaag cagacaactt cgatgtatgg ctgactattc
    39961 tcttctcatg gtcagcatac cagctaagta tttccgcagc ttgtacttga tttccaagaa
    40021 ctttgcccaa tgttgcaata tcctcgtagt attgctcagg tgtctcgagc gtcaaataga
    40081 gctgacggat tccaagcgca ttgagcggcg cttcatactg acttttcgct atcgatttaa
    40141 ggatcaccag gtcaggtttg aacgaggcga tcacttctac cccagcactc ttgtcaaagc
    40201 agaccttttc cttgaaagcc ggatcgactg ccgacataaa gagccccaag ccttgatcgc
    40261 cgcttgaata tgctacaagt ctctctcctg cgtcaggaaa aagataaatg gcatctgcaa
    40321 gcatattggc tccgcgccct gccaagacaa tacgctgttg agcgttgagg ctaaatgcca
    40381 taacaatagc taaaacaagc ccaaaaagaa ttatttggcg tttcataaag gaaaatgtag
    40441 attatctgga tggcaaggtc aagaatagta tgaaatagaa caaagtttgt tgagtttatt
    40501 ttaatatgag tgcataatca tagccagctt ttatatcctc aacatcttct atgattttta
    40561 ttggtaaata tctagtggtc gttgttccat caccaagttg accgtattcg ttagatcctg
    40621 ttgcccaaag actgctatca ttcttaagta tcatcgtaaa ataagcgcct atagctactg
    40681 ctttgacatc atccatgact tttactggac tcgatctatc atgagttgtc ccgtcaccga
    40741 gttgaccgta ttcgttagat cctgttgccc aaagactgct atcattctta agaatcattg
    40801 tatgttcaaa tccagctgca actgctttga cattatccat gacttttact ggactcgatc
    40861 tatcatgagt tgtcccgtca ccgagttgac catgataatt acctcctcta ccccataggc
    40921 tgccatcatt tttaaggatc atagaataac tatcctgtcc aggttcaatt tctttaacat
    40981 cgtccatgac ctttattggc ttcatcgggc cataacctgc cccattgtaa tccatatcat
    41041 cccaacccat tcctgctgcc catgaactcc catcattctt cagaatcatg gtttgagtcc
    41101 atccagctgc cactgctttg acatcctcca taacttctat tggaatatat ctagtgatat
    41161 ttgttccatc acaaagttga ctgtaaaaat tatcacctgt tgcgaataat cttccatcat
    41221 ttttcaggat cattgtatga tatcttccaa ctgatactgc ttggacatca tccataactt
    41281 ttattggcgt tggccttcta ttagtttccc catcaccaag ctgaccctcc gaattcctgc
    41341 ctactgccca taagctgcca tcattcttca ggatcatcgt atgttcttct ccagctgcga
    41401 ctgcttttac atcctccatg acttctattg gaatgtatct attggtggtc gttccgtcgc
    41461 aaagctgccc ataattattg cagccaattg cccacagact aagcagatgc catattgcat
    41521 agagtgtgat gttttcttga ctaaccacaa gagtcgatcc tgctctaaga tttatacctg
    41581 ttccatcgct tcttgtattc cagctatcaa aaccatatcc agctttgatc aagcttcctg
    41641 accttttagc aactattgca ctttcacctt ccagatagat attagaatct atcggaacag
    41701 atccctctgt atttccattt ccattataag ttatggtata accgataata aaagatgata
    41761 tttcagtcca ctttccccat tctccactct gttgcgcacg agctcgccag taataccata
    41821 tccctcgagt tagactttgc aacccaatat ctattttatt ggaggcataa tcggaatttt
    41881 ttaacaagat attgttctca aaatcagatt ggctgctcga gatttgaatc tgatacgcac
    41941 ttgctcctct gacttcagag acgataaatg tagtttgaga accggctatt ctcgaatttg
    42001 ctggataaaa gatcttgata tcatccgcat ttagaattgt atcatagcct tgatagtttt
    42061 cgctttctct atccactgga ttatcaagcg ggccaaaaag atctttgcaa gcaaagagag
    42121 aactaagcag caaaaaggcg agtaaaaata atctgaagac tttcgtctca tttcctatca
    42181 ttttggtggc ccatttctat aggttccatc ctctcctttg aagctcgcta atctgctcat
    42241 cgatatcatc gatggatttc tgcaaggaag aagtatttga ttgcccaaag agaacaagcg
    42301 aggttcccaa ccctgctcct cctatggttg cggctacggg gaacaatacc tgatagagct
    42361 gcacttgcgt tcgaaggctt gctatttcat ctgaatcggt tgcaagattg tacgcgcttt
    42421 tcgtctttgc accctcaatg taggaggcaa ctgcgcctat tgctcctact attcctactc
    42481 caagcgaaag ctgagcattg atctttgtgt cttttgcctt ttcaaggcgc ttagcagttt
    42541 ccgctctttg agcttgaagg ctagttatga tctgcgcaat gtgatcggta aatgaagtta
    42601 cgctgtcttt ttgtacctcc acatatccag tatacgcagt accaggagca cagttgattg
    42661 ataagtaata ggtgcctacg ggaagccatg gagtaataaa ttcatcatct attgatggga
    42721 acacgggata gcttcttcct cccacattga tgtaggagat tccatcagcc aatgggattc
    42781 gaatttgacc cgcatcgagc tctaatttca gagtctggcc ttgttctaat ttgagagtcc
    42841 tagtaggtat agatatactt tctccgcttt tatacgaaga aagctcaaac tccaacttga
    42901 gaggcgtatt tattggaaga ttatcgtagt agataagccc atccttttcc acggttgcat
    42961 tccagtactt atcatttaca tacattgcta gatctacgcc ataattcttc attacaattc
    43021 tcgcgggctc ttgcataaag agacttactt tcaccactgt attcttccct ggctgcacat
    43081 cgatctcgat ctctgaatcc ttgtatccgc tctttgtgag aacgccatgg tatagcccag
    43141 gaggaatgtc ggaaatctgt ccctcggcag gatagctcct tgtaagctct tttatgaata
    43201 atgttgaacc ttttgtcagc acatcaaaag aaataaagcc attcttatat ggctcccatg
    43261 ataccacttg gccttttctt acgtttagaa aagcactggc tgaaacatag tcttttccgc
    43321 tcgcttctat atagtaattc ccttctggca aattccgcac tatccctgac ccctcaatga
    43381 ttctgatctc tgatcctatg tgcaatttcg ctaatgcatc agtggggatt ctaatgtcta
    43441 ctgagccaac aggagtcaat gtcgcggtca tctgcgtcgt cgtatctgct ctaatctcaa
    43501 ccttctcgct atagtaggaa tctttcccca gaagttcaac attgtgtgag cccaagggaa
    43561 gagagcggaa tatccctgag ccaagcgacc ctctactctc tccatcgatg agcacctcca
    43621 catcgctctc attagtgata atgacaaggt ttcctctaag aggcgccatc gaaatagtga
    43681 tctcccgcaa ttctcctggt tcaagggtaa cttcatcggt accactcgag gtcgcggttt
    43741 ttgcagatac ctttagtggc tttttcggct ctaccttttg aatcaataaa ggggctttac
    43801 ctttatacac atcatttagg tatatctctg aatcaggttc agaagccctc actaagaggt
    43861 cagccgcctg tgaggagctc aatatcactg gctcaatagg cttttgcttc tcttttagct
    43921 cattaaaata gaatgactta aagaattgct tatagactgc aggattctgc tctccatagg
    43981 ttacctcctg tacggctgag caggttcgat tcaatacttc ctcaatctca aggccaggga
    44041 ctttcagatt ctttaagagc tccgcagtga atattccatt cctcccagag ccttcgcgag
    44101 caacactgcc tgcagaggtc gcatagacga taatgctccc tggaggctgg taggacagaa
    44161 ctgataaacc tctagaccca gaccgtgccc agctaaatgg attatctctg caggcatcga
    44221 gaatgatgat attgagatta ttcctcgcct tctgaagctc atccaaaacg ctctgcacag
    44281 aaagagattt cgtctttaaa aatgtctcac ccgagatatc agcgtcagca ggtatgagat
    44341 aatttactcc gtttgcctga actccatgac cagcgtaata aaataagcca acagcgcttg
    44401 gagaagtgga tacattatta cccaggcgaa gtacagcctc ctccatcttg ggaagatctg
    44461 catccaaaag gactgtcaca ttccagtcaa gttctttcag aagatccgcc atgtcggagg
    44521 catcgtttac agaatttctt aatttcggca acttgctata gttcgcattt ccaatgacta
    44581 aggcatatcg attcgcgctg ttttgagccg atattggcaa tattccgaag aaaagaaaac
    44641 ttaatccaaa tattgctaaa caaatggcaa ttttgttcaa tctcgatctc atatcaagaa
    44701 ttataattct gatttttccg gggtcaatag gagatttaaa agattcttat atgcccaaga
    44761 agggtccaag aaggaccaaa cgcagcctta tattctatat tttaagacct tatttgcttg
    44821 gccgtctcac atgctccgcc acgaattcgc gataggcagc gttctgttcc atgagctcgg
    44881 cgtgtttgcc gaaaccggct atgcgcccca tattcaaaaa cagcactcga tcgacatact
    44941 gaatactcga tagcctatga gaaacagcga taaccgcagc tcgtgcgaat tcaggcattg
    45001 cagcgagcga atgccataag gcttcttcgt tattcgtgtc gagcgaggcc gtcatatcat
    45061 cgagcaacag cagaccagga tagcctgcaa gcgcccgcgc aattgctatg cgctgttttt
    45121 gccctcctga aacgcttgtg ccacgctgtc caagcttggt ttccaatcca tccgaaaaat
    45181 actcgatttc ttctgtgaga cgagatactt cgatgatgcg taaaagctct tcttcggtta
    45241 gaatctggcc ttctcgacaa ccaaagagga tgttttctcg tatcgagcca ctaaacaaga
    45301 gtggatcctg tggcacatat cctatgagtc cgcgaatctc cgtgggatcc aggctccgca
    45361 caggcttatc atttatcact actcgtccct tctgaggttc gagtaatccg agtgcggtct
    45421 taataagtgt ggatttccct gatccaaccg gtccgattat gaggaatttt tctccgggta
    45481 atatctcgaa actgatatcc gatagagctg ctttgcgccg ttgctcatat cgcactgtta
    45541 cattctcgaa atggagagag cggaatacat cactatttgc gatatcgttt ttctttatgg
    45601 attcttgctg tttctgtaac gatactctat tttgatcttg aagcggtggg ctattttttt
    45661 tctgagacaa tacgctattt cgattttgaa gcgatgggtt ttgttttttc tgagacaatt
    45721 ctgaacattg cttttgagat ccagtttcat attgcctcat ctcttcgagc ctatctatat
    45781 tgacaaacgc tcgcttccca aacacaaaga gctgaggaat gtccagcaca ggatatatga
    45841 gcatgttgag ataattataa aaagcgtaaa aagttcctat cgagatgctt cgttttgtca
    45901 ccatcaatcc gcctacgaac acgagagcaa tctgtgccac atagtcgata tactgataga
    45961 ttaggtgcaa aactgtctcg agctttatga cgtccatctc tgtattgaat cgcttgccaa
    46021 gcgcatcctt gaagaaattt gtgtactttt cttcgcttgc aaaagccttt atgactcgta
    46081 ctccagaaaa actcatctca agctggttgt ttatctccga tatggcttgc tcgttcttat
    46141 taacacggtc gtagattgct gcctgtgtcc tgctgaaaac aaggagcatg acaggcagag
    46201 aagcaaggga agcgagagca agtctccaat cgagactgaa cattgcaata atgcaaaatg
    46261 taaccttact cgctgattcg actgctctaa aaatgccaga acacaaaaac catgaaatct
    46321 ttggaaaatc agaaatatcc gatgtgaggc gtgtaattac atcgccggag cgaaatttgc
    46381 taaaaaatct aatgtctttt cgcactactt cgccaaaata tctctttcgg actatgtagt
    46441 cgaagaccat attgagcgcg ccgcgcacac cagggaaaat gcctgcaatg agtccagcca
    46501 ccccgattgc cgcaaaatag agcgcaatgc gcctaacttg aaccatcgcg gcatcttcgc
    46561 tcatctttgt atctattgcg tgctcgagta ggtcgaagag ctgctttgtg agcagaggaa
    46621 aagccactgc gactgcgctc gatagaagtg tcaaaaacag caaaagccac acgagagctt
    46681 ggcgttctcg ccaaacctct cgtatccagc gaagatgata aaatttttga ctcatacaag
    46741 cacccgcttg cctgctggaa actgctgatc cacaagaagt cgataggctg gcaattcttc
    46801 gtagaggctt tcgtgagttc cttctgcaat gagcctacct tcatctatat agaatatccg
    46861 gtcggctttc agaatcgaag aaagcctatg ggcaactacc accattgtcc ttccctttcg
    46921 aagtacatcc atgctctctt tgatatgttt ctccgtggtc atgtctacat tgctggtggc
    46981 ctcgtctagg aggatgagat cggggtcagc cgcaagcgct cttgcaaaag aaatcaattg
    47041 ccgctctccg gatgagagat tcgaacctcg ctcccagaga ttagcctcga gcccatcggg
    47101 aagcatagag acagccttgt cagcatgcac aatcgaaagt gccgatttaa ctttgtcttc
    47161 cgtgatttct tcttggtata accttacatt ttctagaaca gaaactggaa acataaagga
    47221 ttcttgcaac acaagtccta tatgtctccg ccaagctaca atgtcgattt cgtcgagagg
    47281 aatatcatcg accaacacct tgcctctctg aggaaaggca aaaccgcaga taatgcttat
    47341 tattgtagat ttacccgagc cgctcgcacc tacaaccgcg gtaaagcttc cctttggaat
    47401 gacaaacgaa acatcgcgta gcacccagtc ctcatcctcg taagcaaacc aaacatgatc
    47461 gaagcgaatc tctcgctcga aagtcggagc tttcccagag cctcgatttg gctcttccgg
    47521 tgtagccatt atagtacgaa tcctagagag cgaagtgcgg gcttgctgaa gacttctaag
    47581 gctttcggca atttcagcaa tgggacgcag aagcctagct ccatattcga caaagaggag
    47641 tatagtgcct agtgtcatag cgcctgtaag agcctttgga gcgtagagga gaagaagcac
    47701 aacgatgaac attgggccaa tcaaggcatt caggagagac atcatagagt attcgatcag
    47761 cgaaatgcga acctcaagcg acattctctc tttagccgtt gtatccagat cggcttcggc
    47821 ccatcctgtt cttccatagg cttttaagac ttcgataccc tggatgaact ctgtaacctt
    47881 ggcggtgatc tttgcatata gcgaacgggc cttttcatac atcggaaaga tcttcgagaa
    47941 aaacagaatt aaaattatca ggagacccaa cgaaatgccc accatgataa gtgcgagcgc
    48001 aggcatcaag gcaaaggtca ctataaaaat cccgatcatc gtaagtacat ttacaataag
    48061 gttcgcgcca agggtcgaaa acatatcgcg cactctttcg gtgtccgtct ctgtgcgaga
    48121 cataagctcc ccgacaggat gcttgtcgaa ataggataca ggaagcttca aaaggtgtga
    48181 aaaaatatct tgctttatat gagttacaat cgatagacca agccgcgcaa gcaatatcat
    48241 gcctagatag ctcagagctc cagttaccga cactatgaca aggaaagcgg ctgcataagc
    48301 tagaagaagc gaagtatcct ctcttggcac cgcaacatcg attatctgtt tgaggatata
    48361 tggaccggcg agctgtccaa gagcgatggc gatcataaca atgagcccag gaagcgctag
    48421 cattctatag cgagacaggt agcgcataat ggtactgagg taatcgtttg aatctaattt
    48481 tttttgcata aactgaataa gcgaccttca gagagactta ttgctttcag ataacccagg
    48541 ttaatcaata atgtagttca tattccaagg caattagaaa acgcctcatt gtatacttca
    48601 atcgtaatcg agctcaagat gggcttcaat gaaccgcgca ctctccttca gctcacctat
    48661 tcagttcacc tatagttcaa ctatatagag gtccgagcgc cgagcaggca cgatagtcag
    48721 ccccttcggg atccatgccg aaggcgttcc aatagcttgg cctgaatatc ttttcggcag
    48781 gaacattgtg catagctact ggtatacgca atattgaagc gagagtaatg agatcagctc
    48841 ctatatggcc ataacagctc gccccatggt tggctcccca gttcgccatg accgaataga
    48901 catccttgaa aggaccggtt cccgtgagcc tcggtgcgaa ccatgtagtt ggccaggtag
    48961 gatcggtgcg caaatcaagc gtattgtgca cttcaggcgg cagtgtcacc gtatgtcctt
    49021 cggctatctg aatcataggg ccaagacctt tgataatatt gatgcgagtc atggtaacag
    49081 gcatgctgcc ctcggtcaga aagtccgagg aatagccacc tccgcggaaa tacccaaggc
    49141 tcgcatagcg ccattgtgtc gcatcaaggc aggcctttgc ttcttctggc tcgacttgcc
    49201 accatggttt tattgcaggc tgaccattta tccgctgaca gccggtgcca tcgaggcagg
    49261 ttgcacctga atttatgagg tgcaaaaagc cattttcagc tagcccagta ggcttccaac
    49321 ctgtaacgcg ctcgactgct tcggggctcc aatatgttcg cacatctgaa aaaatctggg
    49381 cactgccggt caaaagatgg ccaaacaaca tcgcaactcc attgagggaa tcgttctcgg
    49441 tagcgacgag gtaaggttcg cggatcccat tccaatcgaa actggaattg agtatggtct
    49501 cgagaaaatc tccattgggg aagtggtcgg tccattggcg ctgcccttgg aagcccgcca
    49561 aaatcgcatt atggccgaga gcttcctctt ctcttccctt tctgcggaga tctggattgc
    49621 caaccattag atcgcgcacg atcatcgcca ttttgacgca ggtttcccat actttgtctt
    49681 tgcgagctcg agaatgctgc tctccgagag gattgttgtc cggtccttct ttgcagtagg
    49741 tctttaccca ctgcaaagca cgatgaaatt ccgcttccga atatattttt tcttcgatgc
    49801 gccgcacaat ctcgctcata tcgacatatt cattgcgcat acctaggtat tcgcggaaaa
    49861 aatcaggatt tacgatggag cctgcgatac ccatggcgac cgaacctacg gagagatagc
    49921 tcttgccgcg catccaagcc acggcgatac ccgcgcgagc aaagcgcagt atcttttccg
    49981 caacatccgc ggggattctt tgagcatccg aaatgtcctg gacatcgcga ccatagatac
    50041 caaaagcagg caagcccatc tgagaatgag cggccaatac tgccgctaga tataccgctc
    50101 ctggtcgatc tgtgccgttg aatccccata ctcccttgat tgtcagagga tccatgtcca
    50161 tagtctcaga gccatagcac caacaagggc taacggtaag ggttacggcc actccttctc
    50221 tagcgaattt ctcagcacag gccgcagctt cggctacacc tccaatcgta gtatcggcga
    50281 ttatgcattc aaccggctcg ccagtaggat ggcgaatatt ttcggaaatt agtcttgctg
    50341 ccgccttggc catgcccatt gtcacctctt ctagtgactc gcggacacct ctgcgacggc
    50401 catcgatggc agggcgaata cctatcttcg gtggacttcc cttccaccta ttcttgggtg
    50461 gttgaagaag aaaatctttt gtctgttcca tactgttcct ccaatgaata tgttgcttaa
    50521 cgcactagtg tatgctgaat caatcttgtc ttgaatcgga gtgtctgagg ttcaagcggt
    50581 cctttgcctt cgattctgtc tatgatgaga ttagccgctg ttttccccat ctctgcaatc
    50641 ggctgctgaa ctgcataccg acaatagcgc aataggggtg aatacggcat ctcgtcgaag
    50701 gcagcaaagg tgatctgttt tgctcgctct cctccctcag agatcaagta attagttgca
    50761 cctagatgtg taaaaacatt gactatgaac caagtattgg gcgaatatgg gtctttcatt
    50821 atttcttcca tagctcgata gccaaaagga agtgttggat tgccaaagcg cacatatttt
    50881 tgttcgattt caagacctgc ttcctccata gctctacaat agccttcata gcgctcttct
    50941 gccgtagtga cttcaggatt gcctcctaaa aatccaatgc gatgatgccc ttctcttatt
    51001 aaggcgctga ccgccttgta tgctccttcc tcattgtcga caagcacctt atccgcctta
    51061 tagcccttta gtattcggtc tacaaagaca atcggaatat tgaaggcaga aatctgagga
    51121 agatacgcac tggaaccccc aaccgggata agtacgattc catcgaccaa tcgttctgca
    51181 agatggcgta tctgccttgc ttcttcctct tctgagcctt gactagtgcc aacaacgaga
    51241 gaataacccc gccctgtgag ctctctattc atgctctccg cgatgaacat gaaaaaatcg
    51301 ccagaaaggt ctggcgatag cactccaata gtgtgggtac gacttgtctt gaggctgcgc
    51361 gcgacaaaat tgagtttgta gccaagcgaa ttcatcgcat ctagcactat gtttctcgtt
    51421 tccttgcgga cccgaggatc gttatttaaa actcgagaga cagtagcggt acttacattt
    51481 gccctctctg ctacattttt gatggtaacg gtctctcttg ccattatgcg actccctgaa
    51541 aagacaaaaa catatcattc cgtaacggga ggcacccctt ttttgaggat gatattccca
    51601 tactttacag tctctcctgt cattacaaca gcaaaagcct ttttagccct ctcatagaaa
    51661 gcaaaacgtt ccatacgctg aatcggagga gtttctggcc aatatcggtc taacacttcg
    51721 cggtatctct tctcaacctc gaggtcgagg ctatctcctt tggagggagc catcatgaca
    51781 atcggatcgt cgacatagga atccaggttg ataaggcgaa aaattccatc gagtagatct
    51841 ggaatcctta tgccatctgc ccgtaccacg cgagaattca gtgaatcgcc agtaaaaaac
    51901 gcatcggcaa gcacaatttc atccccatgt cccatcctat agaggactga gagcagttca
    51961 gccgaaatgc atggctttat tcctatcaac atatatactc cttggaattg aatgtaggga
    52021 ttattccagg cgctatcctc gctgcctcgg ggtcgaaaat agccaattcc cgcagagtcg
    52081 gcggctctgc ccctcttttc tggcagtcaa gcgcagccgc tgagttagca agtcgcaaga
    52141 ctgctcgaag attcacctcg ctcaaagaag caagactttc tcgatccctc accttgagcc
    52201 acccaaggcc cgcaagaagt gcggcatgga agctatctcc cgcgccaatt gtatcgacaa
    52261 tctgcacttt tttggctgga gagacaagat ggtgttgttt tgtcaatatc atagctcctt
    52321 gctctcccat cgtaattgcc acaagttccg gccccaatgc cagcactttt tcagctgcct
    52381 ttgctggcgg catatcatag atccactcaa gatctgaatc gctcgccttg acaatggcac
    52441 tgcgacggca tatccaatcg aatctttcga gatattccgc cctcgatttg attatgctgg
    52501 gtctgacatt cgggtcgaaa cttatgagtc tctttttata ctccctttca atgagtgaac
    52561 aaattgtaga acctgatggt tcctgcacca aagatatcga accgacaagc ataaaacagc
    52621 aggcctcagg aagcgctgga ggcaaatcct caggataata gcatcgatcg gcagcccctt
    52681 ctgaatagaa cgcatatttt gcattgcctg caggatcacg ttcaacaaag gcaagagtca
    52741 tggcctggtc ggctcgaaga agaagagatg tgtctactcc agaactctga agttttgaga
    52801 tgattttgtc gcctaaaaaa tctttcgatg ttttcccaat aaaccatgta tgcacaccga
    52861 gtcgcgctgc agcaatagcg ctattgtaag ggcagccccc tggacaagcc tgaaataggt
    52921 ctttcccatg ttcggagggt accatatcga ttagattttc gccacacaca gctatcatgt
    52981 tctattatct ccattgtgca tatatattcc cagtcttcca tgcgaaaagg ggctgcccat
    53041 tccttttggc agcccccttt tcgcgtcaac cgagattgtc gctgtttctc gaatcttcag
    53101 gtcaagcccg ggttagatgg attctcagtc gtacaagagc tgagcaatat ctggctgatc
    53161 catattgctg tagtcataga acttggcgcc agtatcgatg tcggaaacag gctgtcccat
    53221 caccgctttg tacgcaagct ccatagcatc atagccgatc atgtaaggat cctgcgtaat
    53281 tgcgccaaag aagtatctgt tgcggactgc tgctttttgg gcctttcctg catcgaatcc
    53341 aataactacg aggccaggat actgagtcgg cagagcggtg ccgtcattag aagcagaaag
    53401 aatagcacga gcggtgccct cattcgagca gaaaattccg acaatatggt ctctttgcac
    53461 gcgagtaagg accgcttgcg cggtgttggt tgcgtcagtg tccctagggg aagcgggaac
    53521 taccatttcg aggatgattg ctttaccact tgtggggctg tcgggcgcaa tgtaggcagg
    53581 gttgccggtc actttgatgt cactcagaga gcgttttcct tcggtcgtga tcagctttat
    53641 catcatgtcg cggaatccct tgccgcggtc agtgaccgac tggcttgtcg catcctgatt
    53701 gaagtcgatg atagtgatag gatttgaagg ggttgctgcc atgatcttgt ctttgatgac
    53761 cgggaacatc ttctgtgcag caagccctgc ggcagcatag ctgtttgttg cggcggtcgc
    53821 gtaaatcgct cccttcggtg cgttggggac gcccgagtcg aatccaatga ttggaatttt
    53881 acgccgaata gcctcattga gctgatccat gacggaattt gtgtccaagg cagccaagca
    53941 aatagccgat gggtttttgg ccattgcatt tacgagcatc tgaacctgag gctgaatgtc
    54001 agcttctgtg ggcggtcctt caaaggtcat gaccacatga tatttatcag cagcgtcctt
    54061 agctcccttc ataaccgtct gccagaactg atgctggaag ccctttgaaa tcatggggat
    54121 gaaaatcggg cctgcagcga acacaggaac agtcagtgcg gctaaaagga cgagagccac
    54181 aatcactgtt ctcactcttt ttgcactatg catagaaacc tcctttgcta ttcagccccg
    54241 gatttcctcc ggagctcaat tgccaacaaa taagtccgct acgcggacta aacacctgct
    54301 ttatatgtta tctataggat gatccttgtg tttcagcatt tcggaaaact cgtggtctgc
    54361 agctcgctct tcggcgcgta ttctggcttg ttcagccttt tcttctgctt ttttcgcagc
    54421 aaaggtcgct tttaattcgc ggcgggcagc tgcaatttct ttctggagat cggcgacttt
    54481 gtctctatca ccggaagcct tcagtgagga gagctcacgc ttcatggaag caattttctc
    54541 ggacatttct gctttgtaca tatcggctgc ggtttcgatt cgcacttcag aagctttctt
    54601 ggttcggtac atgtctagca acactgcccc aatgacgatt acccccgtga aaaaggtctg
    54661 ccaatgcgct tgaaggttca ttgcaggcag gcctgtcgcg agcaccgaca taataaacac
    54721 accgataatc gttccccata tcgtgccaac gcctccagaa agggaagtac caccgataac
    54781 agccgccgcg atcgcataga gctcgaaccc ctggccctgc gcggggagaa ctgtggtata
    54841 gatagccgca taagagattc cacctattcc agcggttgcc ccagagataa cataaaccat
    54901 catttgccac ttctggacat cgacgccgga aagtcgagca gcctctatat tgctgcctat
    54961 ggcgtaggtg taacgtccga gcttcgtgtt ggaaagaata aggtggcaaa ttattcccac
    55021 gagaagaaga agaatagcgc cggttggtat cattccgcct tcaggggaga gatagagaaa
    55081 aagattctta taccaaccgc caggctgtcc gcgaattgga aaagtggcgc tctgaacatt
    55141 tgaaacgata gaacccactc ccatcgagat catcatagtg ccaagagtcg caatgaaagg
    55201 cggcaacttc agcctcgaca ctatgagtcc attgaagaac ccaaatccag tcccaaccaa
    55261 gatgatgaga aggagagacg ggagcatagg tacgccatgc ttgagcgcga ctccaccgat
    55321 aagcgccgag gccatcatca ctgtcccgac agagagatct atgccgccag agatgatgac
    55381 aaatgttact cccattgaaa tgaacccgat ataataagaa gcatcgatga tattgaccag
    55441 agttgggtag gagaaatagt tcattccaaa aataccgaag aacacgtata agatgatcaa
    55501 tgcggctgga gcgagcgcat actttaaacc ccgttccttg aaccattgca ttcggcttac
    55561 gctaacattt tctgatgtca ttctgttcca ctccctgtga ggacagcttg gcggcccatt
    55621 gtagcaaggc tcataatagc ctcttggctg gcctcttcta taggtaactc gccggtgata
    55681 cgtccttcgc acatcacgat aatcctatca ctcatgcgca gaatctccgg caactcagaa
    55741 gagatcataa tgatcgactt tccctctttc gtgagctcgt tcattagggt atagatctcg
    55801 ctcttggctc ctacatcgat tcctctagtt ggctcgtcga aaatgagaat gtcacagtta
    55861 cagatcagcc actttgcgac gacaaccttc tgctggtttc ctcccgaaag gtttcgcaaa
    55921 agttgctcta tggaaggcgt tttaatgttg agtctatcga tatatttttg ggtcacatcc
    55981 tgcacttttc tcgcatcgat aaagaggctg ctctggaact ggtcgtaagc tgccatgacc
    56041 atgttgtctt tgacactaag gttgatggca agctcaaaac gcttgcggtc ttcggagaga
    56101 taaccgatgc ctttcgccac tgcatctgag ggcgagcgga tcttagccct tttcccattg
    56161 acttcgatcc atccgctctg aacttcgtcg gcaccaaaga tggctcgcgc ggtttcggtg
    56221 cgccctgatc ctatgagtcc cgaaaaaccg aggatctcgc ctttccgaag ttcaaaactc
    56281 acatcgcgta cgagtttgcc cgcattgagc ttgtgggcgc gcaaaaccac aggagcttct
    56341 ggtcccacta agctttttat ctttggctgc tcatagatga cacgtcccac catcatgttg
    56401 attatctgtg cttttgtcac ctcggaagtc ttcttcgtat cgactacctc tccatctcta
    56461 agcacagtga cgcggtcggc tatctgatgg atttcttcca atcggtggga gatgtgaatc
    56521 atccctacac cttggctctt taagtcgcgc ataatcgtaa agagttcggc tatctctgtg
    56581 tcggtgagcg ctgctgtagg ctcgtcgagg atgagaatcc gcaaacgatg agaaaccgct
    56641 ttggcgatct cgaccatttg ctgtttggct accgtcaagc gtccaacttg ctctgtgggg
    56701 tctatattca tcttaagccg agtaaaaagc tcctcagccc ttttgttctg ctcgcgcttg
    56761 tcgagcagaa tgccacccat cttcgtgaat tctctaccga tgaaaatatt ctgcgcgaca
    56821 gtgagatgat ccatcatgtt gagttcttga tggataattc caatgcccat ttcgagagca
    56881 tgcttcggat catgtgcatt gaataggtgt cccatgtagt agatttgacc tgagtctttt
    56941 tggattatgc cgacgagaat gttcatgagc gtcgacttac cagcaccatt ttcgcccaca
    57001 agcgcgtgta tttccccttc atcgaggtcg aaattcactt ggcgaagggc atgtacacca
    57061 gtaaaacgtt tatcgatctt ttccatcgac aataacttgg cagccacgct catgactcct
    57121 tttcttatga tcagccgtga aaagccttgg atgaatctac ctatttcagt cacaaagcat
    57181 gcacctccgc agtgctactt cggactgtat cgatcaggta aacgattaca tggtttttcg
    57241 attctgtcaa gataaaaaat gcaaacgatt acatttttta ttgataaaag gccttgcatg
    57301 tttttcagat tggttgtata ttcaaagaaa gctattcgaa atataatcga aagattttag
    57361 aaagatatta taaagttttc cggaggttaa agatgcttct agatctcagc gagcgagaga
    57421 ggtcgatctt ggaactcctt gctgatcgca attatctttc tgtctctgag ctttcgagta
    57481 ctctaggtgt ctcggaggtt actatacgta ccgacctctc ctctctagaa aaccggggtt
    57541 acttaatgag aactcgaggt ggtgcgctcc cttcaataca tcggagtatt cttgaacacc
    57601 aaaagctgca tatagaagaa aaacaacata ttgccaaaaa agcagctgaa ctcgtctgcg
    57661 atggcgaccg catcatgata gaagccggaa ctaccaccgc tctcatagcc cgatacctta
    57721 ttggccgaca agatgtacaa attgtaacga attctatgct ggccttctct tatgcacgta
    57781 taaatcccct tctcaatatt atactcactg gaggcacatt tcgacgcgaa acagagtcat
    57841 ttgttgggcc tatagccgta caaaacctag acagcttcaa tgctcgccta gcatttgtgg
    57901 gaactgatgg ctttactatc cagcgcggca tgacgacaca gttcaccgaa ggagccgaga
    57961 ttgtcagggc gatgaacaaa agagcggaga ttacatggct tgtcgccgat tcctccaagt
    58021 ttggcaagat tggttttgta agcgtattgc ccttgttagc agtgcatggg atcattacag
    58081 acaatggctt accaaaggaa gcgctagaag cacttcaatc ggaaggccta gaagtaattc
    58141 ttgcataaat tagatagatg aattcaagag agggatagca aaatggcgga aaagatcatt
    58201 atgcctaaat tgggcaacac ggtagaatct agtgttattc tttcctggaa agtaaggcca
    58261 ggggacattg taacgaagga tactattctg tgcgaaatcg aaaccgataa agcaacaatg
    58321 gaagtgcctg caggggttga tggcacagtg ctagcgcttc ttaaaaaaga gggcgacgat
    58381 gtgccagtct tggagaccat tgcaatcgtt ggtcagccag gggaagtctg ggagaataca
    58441 gaagtttccc ctcaagcaag cactgcgtcg atctctcctt ctgcaactgc ttccatcact
    58501 tccaaagaag aaaaggaaac cacaatgcca caaaagcttc atagcgagga gccacattcg
    58561 agcccacgag ctcgaatggc tatgtaccac cttggcatag gtcaagaggc tatcgataaa
    58621 ggcagcggtc ctggtggaag aattctcgaa aaggatgtta tcgaggcttc gatccaagaa
    58681 ccttcgatcc aagaagctgc tatccaagaa atacctgcgc aagaagctgt tatccaggaa
    58741 gattctgcca aaacaccaaa tgcagcacag attcagacta caaaggaaga accgtcaatt
    58801 catgaaagaa tggaattcca aagcatccct ctcataggca ttcgaaagcg catagcaggc
    58861 cgtatgcatg attctgtcca gactactgcc cagtataccg agcacggttc tgcggatgcg
    58921 gagcaccttc ttgcactgag agcccgtctt aaggcacaag agaatccaaa tcttgcacac
    58981 atcactattg gcgatcttgt tctaatggca gttatcaaga ctcttcctga gtttcccgaa
    59041 ttcaatgcac accttgaagg aaatgagcta agactattca agccaatcca cctgggtgtt
    59101 gcggtcgata cgccgcgagg cctcatggta ccagttatta gaaatgctca ggatctctct
    59161 cttacaggcg tttccatgga agtaaaacgc cttgcaaaag cctgtcaaag cggccgcgcc
    59221 gatccgaatt ctctctctgg ttctaccttc acagtgacga atctaggtgc attcggcgtg
    59281 gagtggttta cgcccattgt caatactccg gagactgcga tattaggcct atgcacaatc
    59341 cgacccgctc tagtaaaaac cgacaaaggt atcgaaactt gcatgagaat aggcctttca
    59401 cttactgtag atcatcaagt tatagatggg gcttatgcag cgcgcttttt aaggcgactt
    59461 tccgagatta ttgctgatat agatattatg ccttttttgc tttgatatct tttttgaatt
    59521 tgtatgattt ttatggagaa tatgtatgac ccaatatgat gttataatcc tcggatctgg
    59581 gcctggtgga tatctagccg cggaacgcct tgcggcaaaa aaactcaaag tcgcacttat
    59641 agaaaaagcc tatgtaggcg gaacctgcct caatatcggt tgtatcccca ctaagactct
    59701 tctcaatagc gctaagctat acgctcattt gcatgaagcc tcttcgtttg gcatagaatc
    59761 agaaggctta caggtaaact ggcagcgcac tatggcatgg aaagaagaag tctctaagaa
    59821 actacgaaga tctttggagc tgacgctcag aaaagccggc gtggaaatca ttcgaggaac
    59881 tggaaaattg acggatggtc attcaattac cgtctttcca ccagccggag aaagcgatgg
    59941 attcagatct tcttcaactt cggcttctca atcctctggt tcggggtctt tggaattccc
    60001 cctagttatc aaaggccaaa aaattatcat tgcaacaggt tcctctccaa ttattccgcc
    60061 aattcctggc ataaaagata atccagcagt gctcgattcc acgggattgc tatctgttgc
    60121 cgaaattcct tcgcgccttt gcataattgg aggaggcgta ataggaattg aatttgccag
    60181 cctcttttct tccttaggtt ctaaagtgac tgtcatcgaa atgatggatg aaatcgtgcc
    60241 tgtaatggat cggcagatgg ccatcgagct gagaaaagcg ctaaagaatg tgactttcat
    60301 gcttggtaca aaagtgacag agctcgatgg gaaaaaggtc ctatttgaga ataaaaatgg
    60361 cgaaaagggc tccgtggaag ccgatattat tttgctctct gttggacgca aggccaacat
    60421 cgaagattgg ggtgccaagg agtctggcct cgatattaag aatcgcgcta tagcgacaga
    60481 tgaccggatg cgcacgaatc tacccggcat ttgggcgatc ggagacgtaa cgggcaaaag
    60541 ccaattagct cacgctgcct accgtatggc agaagtagcc gtctccgata tccttgcagg
    60601 tcaaaatgcc cagcacagcg atcaaatatt cgtggcggaa acagtgcctt gggcactcta
    60661 ctcgctgccc gaagccgcag gtgttgggct caccgaacag gatgctcgag caaaaggtta
    60721 cgatatccaa gtcgttcgaa tgccatataa tgtatctggc agattcgtag ccgaaaatgg
    60781 cttttcagct cctggatcca taaagataat catcgagaaa tcgagcgaaa ggcttcttgg
    60841 aattcatctc ctgggagcct atgcgtctga gcagatttgg ggagccgcgc tcgctctcga
    60901 acgaaaacta ccaatctctg cgcttcgaaa tatggtattc cctcatccaa ctgtctcgga
    60961 agtcattcgc gaagcagcat ggagcgtaca agcgtctggc ggcacagatc aatagcacac
    61021 attaaacgaa aactggctta taaagagccc ataaggagtc atattatgcc aaaatcattg
    61081 cccatcaaac ctgaagatgt cagaaaacct gggatgctcg aaatcccttc tataccgatc
    61141 aatcagtaca agcgaaatat caaggaagaa cgaaagcgtt ttggagatga tggtctcatt
    61201 gccatgctcc acgacatgat cgtggtccga gaatttgaaa ccatgctaaa tacatataag
    61261 acaatgggct catgggaagg aattccatac agctacaagg gtcccgccca tctttccatt
    61321 ggccaagagg cagccgtagt cggacaggct gcagccctcg agccagaaga ctttatcttc
    61381 ggttcccata gaagccatgg agagattctc tcaaagtgcc tctctgcagc tcgtaaaatg
    61441 gatgagtcta aactaatatc taccatggaa tcctggctgg atggagaaac actctcctat
    61501 gccaagcgca tgccttatgt ggatacattc tccctcgcgc gcaattttat catttttgga
    61561 acgcttggag aaatatttgc gcgcaaagcc ggcttcaata gaggcatggg cggatcgatg
    61621 catgcattct ttttgccctt tggctccatg cctaacaacg ctatcgtagg aggctccgca
    61681 gatatagccc taggctctgc cctctataag cgcataaata aaaagccagg aatcgttatt
    61741 gccaatatcg gcgatgcttc gatgggttgt ggtccagtgt gggaagccat gatgatggca
    61801 tccatggatc agtatcgcac actatggccg gaatcggtcg gcggcgcgcc tccgattctc
    61861 ttcaatttct tcaacaactt ctatggcatg ggtggacaaa cctatggcga aacaatgggc
    61921 tttggggtgc tcgctcgagt aggagctgga gtcaatccag aagccatgca ctcggagcgt
    61981 gtggatggcc tagatccatt ggccgtagca gatgccgttc tgagaaagaa aaagatccta
    62041 ttggaaggca aaggtccggt acttctagat actattacct accgcatatc tggccattcc
    62101 acgagcgatg cctccagcta tcgcactaaa gaagagatcg aagaatggcg gaaaaatgac
    62161 tctattgatg atttttcgaa atatctaatc gaaaatgcaa ttctaaagga atccgatatc
    62221 gaggcactgc aaaaagatat acatagcacg atctatgaag ccgtcaagct agctacgaac
    62281 gatgaagaat gccctaggcc caccggtgca ttgatcgaat cggtcatgta ttcaaataaa
    62341 aaagagctta cattcgatgc tcggactccg gaacttcttc aggctggcga agataatcct
    62401 cgagtgcagt cgatctctca ccgctctcgc tatgccttcg acgaagaagg aaaagccatt
    62461 cctaagaata agctctatca attccgcgat ggaatattcg aagccctgtt atatcatttc
    62521 aagctcgatc caacgctggc tgcttgggga gaagaaaatc gcgattgggg tggcgccttt
    62581 gcagtctacc gaggactcac cgaagcgctt ccctatcata gattattcaa cagccctatt
    62641 tcggaagctg ccatagtagg ttctggcgta ggatatgcct tgtctggcgg acgggcggta
    62701 gtagaactta tgtattgcga ttttttgggc cgagcaggcg atgaagtttt caatcaggtc
    62761 tcaaagtggc agtcgatgtc tgcaggtcta ttgaagatgc cccttgtcgt tcgtgtctcc
    62821 atcggaaata aatacggcgc acagcatagc caggattggt ctagccttgt cgcccatatt
    62881 ccgggactaa aggtctattt tcctgcaaca ccatacgatg caaaaggaat gatgcacctt
    62941 gcactaagcc atactgatcc tgtgatcttc cttgaaagtc agctccttta cgatgtgggc
    63001 gagcaattcg aaaaaggcgg tgtgcctaaa gactactatg agattcctga aggcctaccc
    63061 gctattcgca aacctggcaa agatctcact atagcaacct atggcgctac cttatacaga
    63121 gccatggaag ccgcaaaaat cctcgaggaa aagtacgggc tttcggtaga agtgatggat
    63181 ctgcgcttcc ttaccccatt cgattacgat ccgcttgtag aaagcgtcaa gaaaacaggt
    63241 aggctcctat tagcttcgga tgcggtagaa cgtggttcat acttgcacac tattgcttcg
    63301 aatgtagagc agcttgcctt cgattggctc gatgcgcctg tcgtcgtagt tggatcgcgc
    63361 aattggatta cgccagccgc agagctcgaa agcatcttct tcccacagcc tgaatggatt
    63421 gtcgatgcgg tgcatgaaag aattctgcca ctcaagggcc atgaaccttc gactatacaa
    63481 actactgggg atattgcacg ccgctatcga attggtgtgt aagttttgaa ggaattgtgg
    63541 cagatacgag aaaaaggaaa gctgcgatga atgactctaa agaactggaa agaatcataa
    63601 atgatgccag aaacgaaccg gtattgcgcc ttgaagcctt agcgcagctt gcggaaatga
    63661 tgggacagcc agctgcccga tctggagaat cgaacaatca cattcatacc tgctacagct
    63721 ttagccccta tacaccttcg ggcgcggcct tggcggcgcg cagggctggc ctcgatgttg
    63781 cgggttccgt cgaccacgac tcctacgcgg ccgcgagcga aatgcgcgcc gcctgtgcct
    63841 tgcttgacat tggtgttgtg acgggcttcg agctaagggt ctcgctatct gaagccgcac
    63901 ggagtttccc cgaaaagaca gccacgatgc tcaccacccg caaactcaac aaccctgatt
    63961 ctataggcat cgtctatatg actgtccagg gcataccagc ccctgcgctc aaaaaagtcg
    64021 aagtctttct ttcgccaatc cgagcggcaa gatataggcg cagcgctctt atggcagagc
    64081 ttgcgaatga tatcctctta tctttaggac taccggggat agatttcgaa aaagatgttg
    64141 tctccaactc gaagtattca gagggtggca cgataacgga acgccatctg ctagctgctg
    64201 tgtcccgatc tattctatcg caggtagaac ctggcaatga gcttatcaaa tggctagaag
    64261 aaaacttgga tcttgtcctt actggttctc agaagcgcaa tctttcggaa tcccagaatc
    64321 cctatctcgt atatgatctc ttgggagctc tgaaggccgg gttcttggag aggatattca
    64381 tccagccgac agaagaatgt gtcgatgtac gagatgtcat aagttttgca aatagaattg
    64441 gcgcaatccc tgcatatgcg tatctcggcg acgtcgccga atctcctaca ggcgacaaga
    64501 gagccgagaa atttgaagac gaaatactag atgaactcat tcctgtgata aaagagctcg
    64561 ggtttccagc aataacctat atgccaccgc gcaacactaa agaacagctt ttgcggcttc
    64621 aagggctttg caaaaaatac aatctcatgg agatttccgg agtcgacatc aatcagccac
    64681 gccaatcgtt ccgctgcccc gagctgttac ttcccgaatt ccgccatctg gatgatgcaa
    64741 catgggcgat ggtagctcat gaagcactga gtggattcga tctgcgcttt ggtcttttct
    64801 ctgcagaaaa tccactcgcc gatatgccga taaccgagag aatttgccgc tatgcaactg
    64861 taggacgagc catcgacttt agagagcctg gctcgcttga aaaacaagca aaacttttgt
    64921 tcttggagaa atagccatga atatcattat caatccttca agcctcgcgc ctatagtgcg
    64981 aggtttcgtg ctgtcgttgg ccgactattc ttgtatggtt cagagtgttg aagcagcttt
    65041 ttctcatgca ggtgagccgg tctttcacag tgctcattcc gatgagcttt ctctcatcat
    65101 agatgggctt gcttctggag caaatatgga tgacggactc gtcgcttcgc ttctcaaaaa
    65161 agcactcgaa ccatttgtaa cacgcattcg agaaggcctt gccaaaggga caatcgaact
    65221 ttcaatctcg aatgcgaagg gcgaatccat tgcacagtat cagcttgttc ctattgcacc
    65281 gctagattcg cgcattcgcc ttgcagtcca tcggcctggc gatccgagcc tcttatccag
    65341 cagcgctcgt tcagaagtgg taaaaggcaa aatctcgttc gtaactggtg gcgcgcaggg
    65401 ttttggagcc gagatcgctc gcggacttgc actttccggt gcgattgtct ggattgccga
    65461 tataaatctg cctggggcag agcgctttgc tcgagaactc tctgaagaaa ttggaaatca
    65521 aagagtattt ccggtcgaga tcgatgtaag caaagaagag tctgttcaaa aagcctttga
    65581 gatcgttgca cagacttgcg gaggcttaga ccttgttgtg tcaaatgcag gcattctcaa
    65641 ggctggctct gtgctagaac aaccagccaa ggaattccgc ctcgtcaacg aaatcaatta
    65701 tatcggcttt ttcaatgtgg ctcagcaggc cgcgcggcta ctgcgcctgc agtggctcgg
    65761 tgctccggat tggtatacgg atatcataca gatcaattca aaatctggtc tggaaggctc
    65821 aaacaagaat gctgcctatg cggggtccaa atttggcgga attggtcttg tgcagagctt
    65881 tgcgctcgag cttatagagt atcgcataaa ggtcaatgcg atctgcccag gtaatttttt
    65941 tgaaggtcca ctttggtcgg atccgcaaaa ggggctcttt gtacaatacc tcaattcggg
    66001 caaagtgcct ggtgccaaaa caatcgaaga tgtcagagcg tactacgagg caaagatacc
    66061 catgcgccgc ggatgctatg gccaagacgt tatcaaggct atctattacc tcgtcgagca
    66121 attatatgag acaggacagg ctttgcctgt aacgggcgga caggttatgc tcaattgatg
    66181 cttattagcc tatggttgcg ctcgttagtc aatttggatt tttttccttg tccactcgtt
    66241 tttaaaagaa aagggaggct ctatgaccga agcgattgct gtgctcgata ttggaatgac
    66301 aaataagaaa gttgttctct attccaatga acttgctttg atcggagaaa agagaaaagt
    66361 tttccctccg ttgatgcgag atggcataga aacgcacgat ctcaaggcca tggaggaatg
    66421 gtttcttgca tcgcttggag aatttgccag gagcttttcc attggggcaa ttgcggtgac
    66481 cactcatggc gccacctttg tctgtaccga tgaaaaggga aatcctgtcg cgccctgtat
    66541 ctattatacc tatgagccga gcctcgaatt tcacgagcgt ttttatgagc gcgctggcaa
    66601 gcctgaggat cttcaagcta taacaggaac ccccaatttt tccgcgctta ttaatcctgc
    66661 aaaaggactc tttttcttga aggagaatta tccgctagaa tttgcaaaag ctcattgggt
    66721 tttgccctat cctcagtact ggggtatgag gctgacagga attgcggccg cagagagcac
    66781 atatttgggc tgccatacct atctgtatga ttggcaaaaa aatgactatt ccagcgtcgc
    66841 agacatgctc ggtattcgag caaaacttcc ctaccctatc cgacaagcat gggaagttct
    66901 aggcacaatc tcgcccgaca tagctgctcg aactggtcta tctttagata cgctggtaac
    66961 gcttggcatc cacgattcaa atgcctcgat tttgcctcat ctcgcctcta aagtgagcca
    67021 tgactttgtt ctcaattcga cggggacatg gtgcgtgctt atgcaccctg tcgataaata
    67081 tggttttgag cctgatgaac ttggcaaagt cgtgtttttc aatcggtctg catggaatac
    67141 cccaatcaaa actgcgatat tccttggcgg caaagaatat gaaacatgga tggatatcat
    67201 agcaaaaagt tcaggctcca aatcctcaag acagttgccc aagcccgata agaaagacta
    67261 cctccgagtc cttgaagaat gcaattgttt tattcttccg gagatagtgc caggatcggg
    67321 gcagttcccg ggttcagtgg cgcgtgcagt ccaaggcggc atttcatatc cgctttccga
    67381 tatcgaagaa ggcaagtata agattgcttt tctacaggat gccaagatag cgcaagccgt
    67441 gctaaatcta tcgatagtac tccagactga agtagcgctc cagagaacag gcataaagcc
    67501 tggcaccgaa atcctcactg aaggagggtt tagaagcaat acagattaca atatgctcct
    67561 cggatcatgt tttcctggga atgcggtata cctgaccgac cttcaggagg cgacaagctt
    67621 cggcgctgcc atgactgcat tgactgctct aaagaaaata gaccctagag cactgtcgcg
    67681 ctatatccac atagaaaaag tcctagtgcc ttccatgttt gctcccgaaa gattatcggc
    67741 ctatagaaat gcctggcatg cgcagatttg atatattaga ggtaatatat attggaggtt
    67801 cttaaatgaa aacgaaggca gtacgcatct atggtgtcaa tgacttgagg ctcgaggaat
    67861 tcgatctacc cccgctaaaa gatgatgaaa tactcgctcg cgttgtgagc gattcaatct
    67921 gtatgtcttc gcacaagctt gccatgcaag gcgatgcgca taagcgggtg cgtgctcctt
    67981 tggctcaaaa tcccatcatc attggacacg agttctgtgg agagctagtc gaagtcggcg
    68041 caaaatgggc gcataagttt aaacctggta tgcgctttgc tattcagccc gctttgaact
    68101 ataagggaac gctttgggcg ccaggctatt cttatcctta tatcggcggg gatgctacct
    68161 acatcattat tcccaacgaa gtgatggaaa tggactgtct tttcgaatat aaggctgatg
    68221 cattttttat gggctcgctt tccgaaccgg tatcttgcat tgtgggcgcg taccatgcgc
    68281 aataccacac gcagccagga tcctatgtgc atgaaatggg cattaagcca ggcggatcgc
    68341 tcgcgctcct cgcgtcggtt gggccgatgg ggcttggagc tatagattac gcgatccatg
    68401 gcgacataaa gcctgcgcgc attatcgtca ccgacatcga tcaggcgcgg cttgatcgtg
    68461 cacaaaaact catctccgta gaatcagcgc aaaaagaggg catcgagctc atctacgtca
    68521 atacgaagga cctcccggat ccagtagcga ccttgcgcga gctcaatggc ggcaaacttt
    68581 tcgatgatgt atttgtcttt gcacccgtga ggagtcttgt cgaaatggcg gacaagctac
    68641 ttggaaagga tggctgcctc aacttctttg cggggccaac ggatcctaat ttctctgcaa
    68701 atttcaattt ctacaatgta cactatgaat ctcatcacct tgttggaaca tccggcggca
    68761 ataccgatga tatacgcgaa tctctcgaac tcatggccaa aggactcttg cgcccagagg
    68821 ctatgatcac ccatgtgggc gggctcaacg cggtcgcaga aaccacgatc aatctcgaca
    68881 agatcccggg cggcaagaag ctcatctaca cccataagaa gctcgacctg gtagctattg
    68941 ccgattttgc tgcgcgtggc acaaccgatc cattctatgc agagcttgcg cgaatcaccc
    69001 aggcgcacaa tggcctctgg tgcaaagaag ccgaagatta ccttcttgct catgcgcccg
    69061 aaatctgatt gtgtctcata tgatcggaaa ctttattcaa aatcaaatcc ttggaatgaa
    69121 ttggctcaat gagattatcg gaagccttct ttccgttatt ggcctgaaca cttcatccca
    69181 aatcagcggc agtgtgcaat ttttcctgta cgatgtcatc aaaattacat tgttgctctg
    69241 catccttatc tttattgttt cgtatattca gagctatttc ccgccggagc gcagtaggaa
    69301 aatcatgggg aggtttcatg gtttctgggc ttcctgtgta gctgcgctgc tcggcacggt
    69361 cacgccgttc tgttcctgtt cttcaattcc acttttcatg gggtttacga gcgcaggcct
    69421 cccgctcggc gtcactttct cctttctcat ttcttcgccg atggtggatc tcggcagcct
    69481 tgtgctattg atgagtatct tcggaaccaa ggttgcagtg gtgtatgtgc tgctcggcct
    69541 cgtggttgcg atttctggcg gcatgctcat cgagcacctg cacatggaaa gctatgttga
    69601 agattttatc aagaatgtga gcagtgttga aatcgattcg cctgatccga cgcagcgcga
    69661 aaggctcgct ctctccaaaa accaagtcgc atcgacattc aagaaagtat tcccctacat
    69721 tttacttggt gtgggtatag ggacagtgat ccataattgg attccggaag cgtggatttc
    69781 ctcggtgctc g
//
LOCUS       DAOSOB010000002        62134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_519_length_62134_cov_61.126033, whole genome shotgun sequence.
ACCESSION   DAOSOB010000002 DAOSOB010000000
VERSION     DAOSOB010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 62134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62134
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_519_length_62134_cov_61.126033"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2314)
                     /gene="greA"
                     /locus_tag="PLS88_00250"
     CDS             complement(<1..2314)
                     /gene="greA"
                     /locus_tag="PLS88_00250"
                     /inference="COORDINATES: protein motif:HMM:NF011309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOU05824.1"
                     /translation="MDQSPLSEKLQLMLNEEKWTRTLMANYSVANLKELDRFIEEIMA
                     QNGADDIIKICVEHLSQTKNSVIALYVAGMLNIMRQPADESYLVQLMDLFAESKKSNI
                     VEYICNRILDFGENRIALVRLAECYTAENKIEQRYAVWESLVRIDTEEAELAQLIAEW
                     KEKNGLTEEAIDFYKKALIRYINKGLFSNIKELWHKLIQLSPEDYDFFIHAQKKIAKQ
                     VSPEKAATILMDLYDWYKKNEKWDTALSILKLIVEYDERNPALRKEFLECYSNIYSGH
                     SMFDECVKLSNLTQSYRSLHEAIADFEKRIAFDRGNYVFHRTWNIGRISNITNDEVVI
                     DFAKSRNHHMSLKMAFDSLITLSKDHIWVLKATWPREKLREKVKADPAWTIKIIIKSF
                     DNRADMKRIKAELVPSVLSISEWNSWSTRAKEILKTDPMFGNAQDDISTFIVRDRPLS
                     FEEKIYAQFKAEKNFFSRVQYFRDYLEMSEEVDTDYFEEMLSYFVSFLKLTQVNEFVV
                     GSYLLLKELSGRYPRMQQYVTMRFVDLFDQIKDNIVAVYLGLKDTELKNQFIQNIKLF
                     IPSWPDIYIQLLPYARSVKMLDALESNGHEEKLKRMAMYIVENYKDMREAFIWLAKDL
                     KDKPWMKELGIVREKIILTLLHILDITFKEIDSHKDTTENRKINKLVQNILFKEKDLE
                     EFLQEAPQQVVERVYSMLSDIKEVDPAIKLDLKKKISERFPNIKFYDGNERLTVSRGL
                     MVTANKYDEKKRLLGHIMEVDVPANQKEIAY"
     gene            complement(2333..3289)
                     /locus_tag="PLS88_00255"
     CDS             complement(2333..3289)
                     /locus_tag="PLS88_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05825.1"
                     /translation="MAHLPRLKEEATTAEENLDLAYESLKNADMQSADFFLENALKAD
                     FEHPEVLFAMKCLHFWKDLISQMPETGNLLERGDFLCSSWKKFMLFLSHIGDSFDTAR
                     YVYRRFIFGLALDQYLAIPDEEKDSLGPLLDLRLGRARKATGDIETALIHLERAAQGR
                     RNDASILAELADVYAMSGEARLSKALFREAFFIDPQAIEIDFLESELIFKIIENIKEL
                     GYSGDDIAEWIPVYAEIWGVFNIKRELSSAEFNRISASARQLEIQLRESLQQGSSLVP
                     RLLNKYFWMADHLKAREDAKELHSVLLKIKIIDQSIYESYIE"
     gene            3447..4127
                     /gene="rpe"
                     /locus_tag="PLS88_00260"
     CDS             3447..4127
                     /gene="rpe"
                     /locus_tag="PLS88_00260"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOU05826.1"
                     /translation="MKEPIIAPSILSADFYDIGSAVRYIEESGCPWLHLDIMDGHFVP
                     PITFGHKMIADIRAKTRLFLDAHLMIEEPERHINAYIESGADAITFHSEACVHSHRLI
                     QSILHAGIKAGISIVPSTPVSSIIPLLESVDLVLVMTVNPGYGGQKLIPFCLQKVEEL
                     RRIREQEGLNFLIAVDGGINVKLIPDLVIQQPDVLVIGSAFFSAQDKKQFVTNFLDAW
                     ISPIRNID"
     gene            4188..5042
                     /locus_tag="PLS88_00265"
     CDS             4188..5042
                     /locus_tag="PLS88_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU05827.1"
                     /translation="MKKLQMNMLMIVMLIVSSFTYAQTTKTSDEFSYALGLFKNGDFE
                     RAGIAFAEIYQTASDIEILADSCFMAALSYFQATDFPTSYIFCDKFMQSFPSHPNMPD
                     IQFLQGRILFKIGLYEDAILVFDTFMNKYPDSPFSSSVLFWKGESYYQIGDFAQALSI
                     FNKVKETWPNTEATSLTAWRITVMALESREAKYARLAEFESRRNMYRDIQQMQEDVYG
                     EQRLLRDYLWSKAISRYSATPNIEIQKAIIDNISRNDRLNRLLDAKERALKLVILKIE
                     NYLKDIAP"
     gene            5039..6103
                     /locus_tag="PLS88_00270"
     CDS             5039..6103
                     /locus_tag="PLS88_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05828.1"
                     /translation="MKKSIFFFILSFIFTGALFAIDIRSGLVRIATDDQTLQPMLYRL
                     VNISGKSKYEPLWFDGDPRTSFIILNIDNRIYRMGASKEYKTSQRQIKNGIEIEYRSV
                     TNRLTQRVLFYKSPYSRVTDGFIIEIEIENYTTREMKVLIKEVCDTVLGEKGGYNFAT
                     SSNPRVTDEMIITSDSDEKYVISPGQTSSIALLLDEEAKPDMVVIANWKRLSDSKFYY
                     DSELMKGFTQSPFSINDSALGLYWNERIIPPKGSIKVASTWLTGGPGNEMIDWLSQNY
                     PFPNQELQQAFENPPVVFEEQPAANVPKLDIEAIKELISEIDRAIGNIDNVSDKEIQD
                     ILSRLNCLEQSEEFAASQSN"
     gene            6120..6581
                     /gene="dtd"
                     /locus_tag="PLS88_00275"
     CDS             6120..6581
                     /gene="dtd"
                     /locus_tag="PLS88_00275"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOU05829.1"
                     /translation="MRAVIQRVSEAFVTYKNTKEDKFCGRITKGILIYLGIAADDTDG
                     DAEYLADKIAHLRIFMDSNEKMNLSILDLGYEALVISQFTLHADARKGRRPSYSQAAG
                     PEKALILYDLFCTKLMEQGVFVQTGKFQEIMRVHSINDGPITILLDSKKAF"
     gene            complement(6671..9457)
                     /gene="ppdK"
                     /locus_tag="PLS88_00280"
     CDS             complement(6671..9457)
                     /gene="ppdK"
                     /locus_tag="PLS88_00280"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOU05830.1"
                     /translation="MPRKKFVYAFGTGLAEGDGTMKELLGGKGAGLHEMTKIGLPVPA
                     GFTITTEVCELYYKNGKKWPEGLEAEIQKHIKNLEKLTKKTLGDPSDPLLVSVRSGAP
                     VSMPGMMETILNLGLTDASVEGLASKTGNRRFALDAYRRFIMMYGSIAMGIDREVFDK
                     AFEEIKETKTRLRLGIPTGQKVSDTDVNEEELSELIKYFKKIYREHIGTDFPQDPWEQ
                     LKGAINAVFDSWMAEKAVTYRKVEKLVGIKGTAVNIVQMVFGNKGENSGTGVCFTRDP
                     NTGENIFYGDYLINAQGEDVVAGIRTPIKLAELEKRDPKVYRQLVDIRRKLERHYRDM
                     QDLEFTVEEGKLYMLQCRTGKRSPIAAFRIAVDFVKEKLITKEEAILRIKASDIEGIF
                     YPMIDKSNPAALKKSFLVQGIDAVPGAAVGKVVFNAKVAEEWEEKGEKIILVRKETSP
                     EDVGGMHAAQGILTATGGKTSHAAVVARGWGKCCIVGCEKLEIDYENGQFAVGNTIVK
                     EGDYITLDGNTGNVYLGQLKLVKPEPPKAYTTLMSWVDRTRTLKVRTNADTPYDAKKA
                     RELGAEGIGLCRTEHMFFDSEERILAIREMIIADDTESRKKALAKLLPFQTKDFEGIF
                     KAMDGLPVTIRLIDPPLHEFVPHDEEGQRELADAIGVPFEKVVQRVEQLHELNPMLGH
                     RGCRLTITYPEILDMQVTAIITAACNMTKNGIKVLPEIMIPLVMDDKELKILESRARE
                     IAAKIIATSEVPLKYMIGTMIEVPRAALLADRIAKVAEFFSFGTNDLTQMTLGLSRDD
                     AGKFLPDYIDEKKGAIFRADPFQNIDQDGVGMLIRMGIEKGRTSRPRLKVGICGEHGG
                     DPESVKFCHRVGMNYVSSSPFRVPVSRLAAAQAVIEDAQAAHKTARKPGRPAKATSSK
                     AKRPRGRPRGSKNR"
     gene            complement(9630..10271)
                     /locus_tag="PLS88_00285"
     CDS             complement(9630..10271)
                     /locus_tag="PLS88_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05831.1"
                     /translation="MRNLWKILLSLALIISLPFYFISCIGIEASTKIDSKGAGTFSVE
                     YQIAKDFVDFGSEESTPSLPFALSREDIERGLQDTEGISLKSYSKTKNANNMIISFTL
                     DFDSPENLVKYLDPTNSFARFNKENGIYSLKFSFGDEIPPLDPDMKKYISEQFASYRF
                     SLTIETAKSPPEVFIQDGSFISKTTSGKKTILECAMADLLTVSTSPQIDIFWR"
     gene            complement(10285..10563)
                     /locus_tag="PLS88_00290"
     CDS             complement(10285..10563)
                     /locus_tag="PLS88_00290"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOU05832.1"
                     /translation="MLRLVLRLNETSTIKDKRRVVSGIKDRLQHRYRLSCAEIDLLDS
                     LAFAEIGAALVSNSAQYGEKVLRDAVTFIEDHYPVELYEVQMHYEIYG"
     gene            10642..12891
                     /gene="metG"
                     /locus_tag="PLS88_00295"
     CDS             10642..12891
                     /gene="metG"
                     /locus_tag="PLS88_00295"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOU05833.1"
                     /translation="MKRRLITSALPYVNNVPHLGNLIQVLSADVFARFCRLRGYETLY
                     VCGTDEYGTATETKALEEKVSPRELCDRFYEIHRDIYEWFEISFDTFGRTSTPEHTEI
                     TQSIFFDLDKNGYISSQSIDQLYCDTCGRFLADRYVRGTCPYCGYEGARGDQCEMCGK
                     LLDPMELLSPKCISCGGTPRPRSTKHLYLDLPAILPKLEAWMKEASVRGFWANNAIQM
                     TQAWIRDGLHPRAITRDLKWGIPVPKEGFKDKVFYVWFDAPIGYISMTARLAKEKGFD
                     WKSWWQKPDEVELFQFIGKDNIPFHTVIFPSSLLGTGKNWTMLHHMSSTEYLNYEAGK
                     FSKSKGIGVFGNDAMNTGIPADIWRFYIFWNRPETSDYTFTWTDFQEKVNGELIGNLG
                     NLVNRTLTFISRYYSGIIPEGTPDMAFWKEVADREQEASELLERADLRDAFRTIFSIA
                     DLANKRFQYEEPWKARIDNPQKASSLMRDLCYVLKDLAIMTYPYMPSTAKRLASFLGI
                     EIGQGKLGWADLGLKGEFQRIKKHEVLFNKLEDELIASLRVQYSGSQKEREEKEEQNR
                     KNEGKLPASIEKNQNNRFDQIIDLRVARIIKIERHPKADKLYIETLDDGSGSERVIIS
                     GLVPFYREDELLGKNIVLVNNLKPAKLRGIESAGMLLAASTKSLEGNEIVEVLTAPNA
                     KPGERVLLEGSSIENLEETISKENIDIETFLTIPLYAKDGYAWSEGQRLMVRGEPIRL
                     EKVMDGKIG"
     gene            12930..14192
                     /locus_tag="PLS88_00300"
     CDS             12930..14192
                     /locus_tag="PLS88_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOU05834.1"
                     /translation="MDVLPKIEISPILSAMDCMPECHEQGFLQSKFWGLFKARTGWQA
                     YSCTYSFYDKDLSGRILVLKRKFAKLFSFLYVPFGAGELSSISKRWNALSALGRALGE
                     SFGGTDVFIRFDLPWERDKALAKNKEEEEKLDIESTLGFRIPGLYKGTDVQVPDTVIL
                     DLQGEEEQILARMKPKWRYNIRLSQKKAISVEDEGISSLPLFMSLYKETAKRDRIAIH
                     PESYYKTLFETSSEIAEAARANSIATITPHLSLYVARHKEEVLASIIVLHFGDTSTYL
                     YGASSSKKRNLMPTYALQWQAILDAKRSGAKKYDFFGIPPDDKDDSHPMAGLYLFKTG
                     FGGNIIHRYGAYDISLRPLLYSVFRTAEKTRSLWHKKIKKTLNKKTVRLSDETMKRLT
                     IKKVQKSQESEEDASLSSDSENTKPSNP"
     gene            complement(14126..14698)
                     /gene="amrA"
                     /locus_tag="PLS88_00305"
     CDS             complement(14126..14698)
                     /gene="amrA"
                     /locus_tag="PLS88_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOU05835.1"
                     /translation="MEFTISLEDQKKLLNYARLTINASLKGEEEVEPPLLSKSLKCGA
                     FVTLRKNGSLRGCIGRIQSDADLTKTIKQMALAAAFEDPRFPPLKKEELPLIEIEITL
                     LSPLYPISPEEIVIGKHGLLISAHGRSGILLPQVPIEFGWDKETFLEQLCWKAGLPSD
                     MWKSPLSQLYGFEGFVFSESELKEASSSDS"
     gene            complement(14702..15523)
                     /gene="amrB"
                     /locus_tag="PLS88_00310"
     CDS             complement(14702..15523)
                     /gene="amrB"
                     /locus_tag="PLS88_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOU05836.1"
                     /translation="MSDEKLVRSPLLSGIFYPSDPDMLMSEMDNLSMYAKPMPCPAFA
                     IIAPHGSFLYSGKVSMESWGAILGADPSIIAIMGPSHLPYEEGVFLPESTKFDIPGTS
                     LNIDSNLRDYLLTKVTMLKKNDLPHLEEHSIELQLPFASRFFPGVPILPCIISGKNPS
                     TIETATRLFEEISNYIGNQVIFVLSSNLAVSDSPEKCDSLSKEFVDSLSRPIAEFHVE
                     AESCCNSFCGEAVIRAFRVAFPKARAELLDYDNSSKHREYCDELVVGYGAIDFKR"
     gene            15643..15963
                     /locus_tag="PLS88_00315"
     CDS             15643..15963
                     /locus_tag="PLS88_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05837.1"
                     /translation="METVSQALLYEEKLLRNRLQRVESECASRPKGSIVLKRRYNQVY
                     AYLQWRDGNRVCSRYLGKPDSWQCRSIQAKITERRKYLEEAKEIRKKLEATQHLLEEV
                     RKFS"
     gene            15996..17144
                     /locus_tag="PLS88_00320"
     CDS             15996..17144
                     /locus_tag="PLS88_00320"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOU05838.1"
                     /translation="MKDIFIIHEEEYDTAPDCTSAAPTIVKILGEHTDFTDGLVLSAS
                     LSFEVRVAISFRKDNSLRFYAADFNERKRANVATFKYKREDRWANHVKSVCDYFWRVF
                     NLDPRGLNVTIQSNVPLGLGLGISSAINMATALIFKTLYDLDLKLEELAFHAWKAQSL
                     FFEKELPITNYLAITAPAGRSFSIIDLHGKKRRGVQFLPEPWEMLLTDSKVPRSSVEA
                     ELEQRIADCKACISVLVPYHNRSIRDIAPQELDELLGIVPERKRRRCLHVLEEVRRVV
                     EAEESLSRQDYVGFGRIVNKSHASLRSLYEISCPEIDWLTKRALELDDVLCSRLVGQG
                     FGGSTLTILKNEKKQVYRNKLEDYERIFGFKPIIHEVSTGAGLRILEH"
     gene            17183..17944
                     /gene="surE"
                     /locus_tag="PLS88_00325"
     CDS             17183..17944
                     /gene="surE"
                     /locus_tag="PLS88_00325"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOU05839.1"
                     /translation="MRILLTNDDGIQSDGIFALIDTITAMKDANGKSLHQVWVVAPEH
                     ERSGVSHAMTLKKPAKLRRLGDFRYSCSGTPVDCIIIAGLAILHEKPDLVISGINRGP
                     NLGTDIIYSGTCGAARQAALQGIPAIAVSCAAYCDNLEYEGLTSFLSGNLEELIALWK
                     PDSFININGPSTKSRDLKAVWTVPGKNKYIDNLKCFDGLDGYTYCFLTEGGEERTPDN
                     LSDHHVISQGHIAISLIEVHPRAFHESSLNGKFVF"
     gene            18039..18111
                     /locus_tag="PLS88_00330"
     tRNA            18039..18111
                     /locus_tag="PLS88_00330"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18072..18074,aa:Thr,seq:tgt)
     gene            18135..18217
                     /locus_tag="PLS88_00335"
     tRNA            18135..18217
                     /locus_tag="PLS88_00335"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18169..18171,aa:Tyr,seq:gta)
     gene            18317..18802
                     /locus_tag="PLS88_00340"
     CDS             18317..18802
                     /locus_tag="PLS88_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOU05840.1"
                     /translation="MNPEMPIKKIYFECMRCNACCGGQPGYVWLSKKDLMALSEYFGL
                     SIQEFALAFCRMVDIGIEITLSLKEKQNYDCIFLENYGCSVYPVRPVQCKTYPFWETI
                     LENEQSWIEEAKYCPGIGKGPLISSNTILDAFLERRQNTPLSIDEITALRALENKWDN
                     Q"
     gene            18799..19815
                     /gene="mltG"
                     /locus_tag="PLS88_00345"
     CDS             18799..19815
                     /gene="mltG"
                     /locus_tag="PLS88_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOU05841.1"
                     /translation="MKKYHIRFIMQNLLILFLILSMAIIILIITPPFRSKSVEVKIKS
                     GSSLRDVAQELHNSHVIPSSRIFELYVRAIGSERSIKSGTYRFMPFESPIAAASKLIR
                     GYYITQKVVIPEGRTARQIATILESNNVINAEAFLEAVNDTELAKSLGIPASSCEGYL
                     FPDTYFFNPQCDSRELVSQMVAGFNDAIERIEGTKTKLLNPQSLHQRVILASIIEREY
                     RLPEDAPLIASVFFNRLQRNMPLQSCATVVYVMTEHLGKEEPSVVHYGDLKIQDPYNT
                     YINRGLPPGPISNPGEISLRAALFPPQTDYLYFRLDDVNTGKHRFSHTLEEHNYVEII
                     PKGL"
     gene            19822..21612
                     /gene="dnaG"
                     /locus_tag="PLS88_00350"
     CDS             19822..21612
                     /gene="dnaG"
                     /locus_tag="PLS88_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOU05842.1"
                     /translation="MARISPQIRQEILDKTDFLSVYQEHVRLQKKGASFWGLCPFHSE
                     KTPSFSVSPDTGLFYCFGCHKGGSIIQFIMDIDRLSYTEAMQELAQRCGVQIRFEESE
                     ELGETEKERQALLELNERLSKTFHWFLMNHDFGRPALEILHKRGLSDELIEKFSIGYA
                     PANRQWLYSFLKAKGYTDDFLKHSGLFSKKDLAYPLFADRIMFPIGDAKGRIIAFGGR
                     AMNEDGPKYINSPDTAVFRKQETLFAFSQAVDSIKNSDTAIICEGYMDALSFHAAGLT
                     NAVAPLGTAFTSQQAMIIRRKSSNIILCFDTDEAGLHAAERACSIATATGLETRVLLL
                     EEGKDASEILEKKGAGELTRMAKNTINAGYFLLTRANQLFDISTMEGKAKAVSFLFPF
                     LDALDSDVKRQEYIKEVGRIIGVSSHAVAMDYAKAKVSGMRRTRDTYGISNLPYGSSN
                     SARTSDLLFMAAIILSENPFQFIDENLDIENFDDPRAKDLFLALQEAQKEGILDIEGI
                     LSRCTDEAAARFVREVDTSGELQGGLEKIIKDGFEKKKKAALERKRRDLVTQLQSLSV
                     DADPLQEKLILEEIINIDGELKATGGDINE"
     gene            21605..23332
                     /gene="rpoD"
                     /locus_tag="PLS88_00355"
     CDS             21605..23332
                     /gene="rpoD"
                     /locus_tag="PLS88_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOU05843.1"
                     /translation="MNDIELDPAIAKLLEYAKEKKTISFDELSDMLPEQILNSEKMEA
                     IINLLVNNDVKIEEEIQIEEESLPKLTSIPLKSKMVSNDKEISSDDPVRLYLRDIGRE
                     NLLTAEQEVELSRMMEQGENIIKNTLRKSGMLIPEFHQIVVKATRKEPKDISLQKKEN
                     TEKIAERRRLNQFYKDLIDTSAADLKAYIELKKSIIAKGGNIFEDPQLKEIREQLLPI
                     VNETDIHPEEITVFSEKFIVAAKKIIRYRKEQDRIQGILQISSSKDLRTLGRNLTIKE
                     KREEIEQRLGLSAEEIKDKIRQLQVNEKKLQDIENNFETPIDKITSMAREVNRGRKMM
                     KSAKDRLIKANLRLVVSIAKKYTNRGMQFFDLVQEGNIGLIKAVEKFEYRKGYKFSTY
                     ATWWIRQAITRSISDQARTIRVPVHMIEQINKVVRESRQLMQKLGREPTDEEIAAQLG
                     WEIERVKAVKNVAREPVSLDTPIGEDEDSLLQDFIEDKEVENPAVQTNYNLLQEQLRM
                     VLSTLPKREQEVLRMRFGLDEGYSLTLEEVGLYFNVTRERIRQIEAKALKKLRQFKRS
                     QKLRDYIDH"
     gene            23345..24268
                     /locus_tag="PLS88_00360"
     CDS             23345..24268
                     /locus_tag="PLS88_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOU05844.1"
                     /translation="MPMRMDELFETLRKYQKLLSDRVELEKQLEAMPKTIEIQNQMLS
                     REKKSFVDVKEQFTTTETNLKQLRQALAEAEMRRENAERQMDGIQTQREYDAVNKEIR
                     EASEKEQDLRHRIQEEERRFAEIDDKLHRIEISVSALESEIEQKRTEIEQNSKQIIDQ
                     IEKLRTEENSLTQELEDDLKFKFERIIRSKSGMGIVPIHGVVCTGCHMILPANFVNEV
                     RQADKIVFCPYCSRILYYEETEIEGSIEEILTGSLVDLGEEDSEETEETESEDESEDI
                     SNSSKKGSSRKRKNQSEPLIDNDFLIDNMEE"
     gene            24277..24691
                     /gene="rnpB"
                     /locus_tag="PLS88_00365"
     ncRNA           24277..24691
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLS88_00365"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            24709..25650
                     /locus_tag="PLS88_00370"
     CDS             24709..25650
                     /locus_tag="PLS88_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU05845.1"
                     /translation="MFDRFFRGKAPRTRKKNPWLFIAIILIGVGVFLGFYFAISSKDT
                     KNGISRSALAKTWNSGNNIEKTLSEAQKAIESYPFDEYYLSICGIAYYYSALNAQEEE
                     TRQQELEKAVIALRKALAVGLPSRMKGQVYYILAKAYYQKGEPWFDLAERYFLLAKES
                     GSKEKDIPQYLAIVYAGRKDYNRAVSWFEQALENNTSDALLLSAAISYMNTDQKEKAR
                     DLLLRLESSASDAKIRLKASLLLAQDNFDSGDIQAALNRYQIVLKEDPVNVDAWYGLG
                     LIYAKLNDQLGARAAFRKVVQIDPNHADARRRLAEKL"
     gene            25682..26701
                     /locus_tag="PLS88_00375"
     CDS             25682..26701
                     /locus_tag="PLS88_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOU05846.1"
                     /translation="MFSPDIGIDLGTCNTLIYMNGKGIVLDEPSVVAVERGTNRIVAV
                     GTEAKSMLSRTPTDIIAKRPLRDGVIADPDMTEKMIKYFIQRVFPKNLFFKPRIVVGV
                     PTCITKVEENAVIDSTYKAGARMVKVIAESLAAAIGAGIPITEPAGHMICDIGGGTTE
                     ISVISLKGMVVTNAIRVGGDEFDEAIAKHIRSIHNLIIGEQTAERLKIEIGNASPERT
                     IEKIEIKGTDAITGLPRRFEVDSVEIREALMDSVNLIVEEIKKTLGKTPPELAADIVE
                     RGIVLSGGGALLKGLPKLIAKETGVPVILSEHPLECVAIGAGKYFEIIQKNIGSSSVY
                     ESLNL"
     gene            26718..27599
                     /gene="mreC"
                     /locus_tag="PLS88_00380"
     CDS             26718..27599
                     /gene="mreC"
                     /locus_tag="PLS88_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOU05847.1"
                     /translation="MKIGSQQTHGPARVMHKKAGICMMLSIIVLFISTKTIVKVPSFF
                     KGYVIGGIQKSLSAIGSFFTGTVGAVSELRKIEKDYEALLEKTQDYELKVRDYTALRE
                     ENQRLKELLGLSTLPDAKKIAAHVIARDPTNMYSSLVIDKGYINGIRKYMPVVAYQNG
                     IEGLVGKVIEVKASTSILQPFYDQRFFAAARLAKTRAEGMLNGQGFRDVPLNLLYIPK
                     SEMEQLKQGDIVVTSGLDEIFPPEIIIGRVEGWRLNEFSSSLVISVQPAVDLSRIEYV
                     FAIDIQAPSSPGKEAIQ"
     gene            27596..28105
                     /gene="mreD"
                     /locus_tag="PLS88_00385"
     CDS             27596..28105
                     /gene="mreD"
                     /locus_tag="PLS88_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOU05848.1"
                     /translation="MKSILISSIVAALMLFIQSTWLSRGIIMGIIPNLALDVLLFTSF
                     INKEGQGIITSFLVGIVADILSASPLGYYAFIYTSCGYFATLISYVTEKDIFIVPFLL
                     GAVATIIMAILSKVISLLFSAGIHYYQVFSMEFGVELILNGLFAILLFFLLSFMRPLF
                     EHNPKKAMP"
     gene            28140..29993
                     /gene="mrdA"
                     /locus_tag="PLS88_00390"
     CDS             28140..29993
                     /gene="mrdA"
                     /locus_tag="PLS88_00390"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOU05849.1"
                     /translation="MLPKKITLRIRILKALIIMLLVIYSINLFSMQVLRKELFVSEAQ
                     RISIQSTRIPAPRGEVYDRSGRNLLAGNIEAYSVYITPSELPGSKKSEVINKLSLLLN
                     IPIEDIENKLPDSSLYSYGRIVIAKNASLEAVTQIAARIDEFPGISWSSKPLRNYADL
                     QSLSNIIGYVGTITRDEYKLLYNKGYILEDLTGKAGIELAYEDLLKGKDGWISKAVDV
                     YGKDISNNAAEIQEPIPGKRLILSIDSKIQKIAEEALGPRQGSVIVLKPATGEILAMV
                     TYPYYDSRIFMSDNVGKAYLELLNDPNKPMLNRAYQSSYPVASTFKTVLTAAILEENA
                     ISPDKKIYCSGELIYGGRSWSCHIKKPGHGALDLKNALAQSCDIYFWTAGRDYLGIEN
                     IVSYAQDFGYGKPTGIDLPAENVGLLPTPTWKEEKYNEIWTPGDTMNLSIGQGYLLAS
                     PLQLANMMAMIVNDGIIYKPHILKEVRDPTTSALINEIQPEILHQSSISSRTFETLKS
                     YLRGVIVYGTAKVPINTKVVQVAGKTGTAEVGLKDRWHSWFVSFGPYDAPAKDQIVVA
                     TMIEASNPWEWWAPYAANVIYQAIYANQNAHDAAKAVGVRLEGSSLIGKRE"
     gene            29990..31309
                     /gene="rodA"
                     /locus_tag="PLS88_00395"
     CDS             29990..31309
                     /gene="rodA"
                     /locus_tag="PLS88_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOU05850.1"
                     /translation="MKSQSFKRLFGNIDYYLLFSCFLLCALGIVAIYSSGIDAEGVTV
                     SNEYLKQIIWFGIGVFLLIAAAVFDFSRYKDMVWILFAFAILLLIITLLAGKVVNGAR
                     SWLGIGGIGIQPAEFTKIATVLMLARYLESAEFDSSFKKLLMTGLIMSIPLALILAQP
                     DFGSALVFIPAALVMLTLADVDFQYILFGVLSIVGVFLFLSLPLYASNHPNPENILFT
                     AITNKNVLIAILIFILVISIISGIGWLKFKKRYYYWLSYSMAILAISTIGAIIAHSIL
                     KPYQIMRLLVFIDPSIDPKGSGWNILQSITAIGSGGFWGKGFLQGTHSHARYIPQQST
                     DFIFSIIAEEFGFVGCIVLFILYGILLFRCIVLIETSKDRFSQYVIGGLLGIFAFHFM
                     INIGMAMGIMPVTGIPLYFVSYGGSSLWTGLISIGILTGISARRYSA"
     gene            31306..34014
                     /locus_tag="PLS88_00400"
     CDS             31306..34014
                     /locus_tag="PLS88_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOU05851.1"
                     /translation="MNTNFIDPLRDIGEALFEVQKPARYTGGECSAFSSIDPLDSRLR
                     IALSFPDLYEIGMSNNAIRIIYSMLNEQRSALVCERVFSPAPDFEHLLMEKQLPLYTL
                     ESGIPLNKVDVLAFSIGYELLATNILAIMDRGWIPLETKKRGEKDPIIIAGGPAITNP
                     LPFAEFLDAVWIGEAEEGFTELMLRLAKLKSNGATRIEKLKALALHSSIWISPFLQEL
                     GIPTKKKVKRAIYQDFYRTTYTSNFPYPVINPVHSHGSVEIMRGCPNGCRFCHAGYFY
                     RPQRSKFPSIIKKEVEDLIIKKGYTEITLSSLSSGDYPDIIGLFNDLTALWSKYNVSF
                     QLPSLKVDSFTLPLIEKISEVRKSGLTFAIETPLDSWQCGINKRVPLEKIKAILHEAK
                     LKGFKSAKFYFMIGLPVAERGNGEEEAIINYLNNIASIEHIAIHVNIGTFIPKPHTPF
                     EREEQLSEYESLKIIKNIRKNLRSTKNIEISYHSPFLSVLEGLISRGDESIGEIILYA
                     YKKGARLDAWEEYIDPDIWNNALKNYNSKRGEGAWQRFLARRDENENLPWNEISFKIS
                     SKWLKNESKKAKSNQLTLICNDNCTNRCGVCDNKNSIVSYNILYKDIKDSSLPIFSVE
                     GKNIRSINNNDNVSSLDIDKSDGISFINCADILNNYIDFKSTTAIKIICVWEKKDIAV
                     LYPMHDVAKAFYRAFQISGLSIAYTRGYNPMPRIELSPPLPLGVEGENEILIAWINIS
                     QDNISQINRLFSLHQENILYLINLNLPKGLYLKNIKVGVFPQEKRKTIGSLLKATAWK
                     YEFIGKPSYSKAVDILKEYSDFFIPINVTEAVDNSSENEIELLEKFSGSSGKTISFFK
                     ILKENSYSFCFQNKNIESSRKYFSAKRIGCYGYNESNGYDLLQNIL"
     gene            complement(34154..34876)
                     /locus_tag="PLS88_00405"
     CDS             complement(34154..34876)
                     /locus_tag="PLS88_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05852.1"
                     /translation="MSHLIATLFEISIVCFFIIMISCGLPTAEYLFPPDDFSSANGVL
                     VIYHNKENIDEPTISSIFLGYEIYYRVFDDKNEAEQSYYNIRNMTSSNVIQTANYYQY
                     YPLKHRISASGQYSLDISPLIKKESLIKVSSNKYNLNMNTQGTWTLTDEGSNTVIDSI
                     VRNRSDTASSADIVKADFYRSSEYKVGDRDYAGTNSVTPGGSVYIVFFAFAYGFDPST
                     MKNVYSDPVILEPSVEYIPGGT"
     gene            complement(34963..35718)
                     /locus_tag="PLS88_00410"
     CDS             complement(34963..35718)
                     /locus_tag="PLS88_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU05853.1"
                     /translation="MNQEEIQQVQPEKDKEQKKERDIIKKEEKKSSSEKFLDFISKNR
                     KIIIGLGIAIVLIIVAIGIYTAVSGNIASASSRAMELADQKTMQWSQETDEQKRAEIE
                     TSLMAELDSIANKWPKTIAAQRALIRKAAILSQKKEYAEAEKVALEAYKRNKKSYVAP
                     IALELAAVAAEEAGNIDAALEHYILITKDYKKDNPAAPHAFFNVGRIKETKGDYKGAV
                     EVYDQLISSFGDSEWAMLGKNRVIYLKAQGLAD"
     gene            complement(35722..37236)
                     /locus_tag="PLS88_00415"
     CDS             complement(35722..37236)
                     /locus_tag="PLS88_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOU05854.1"
                     /translation="MVSVIDSDRFRTLIDINAQLNSSYTDFRSLLQTIVESAARLVGA
                     EAASLALFDASTESLRFEIALGPRGAELQGKSVHATEGIVGWVFKNRRSIIVNDAAND
                     PRFSPTVSREISYSTNSILAVPLKVKDRTEGVIELINKASHGLFISEDLEWVELFAVQ
                     AGIAFDNARQYEKTEQELTYLQHKVQEGEGWHPLIFSSKVMHDRLELVKRVAPSDASV
                     LILGESGVGKELIAEQLHLLSKRSEKPFIRVNCAALPESLLESELFGHVKGAFTDAIE
                     NRAGRFEAANGGTIFLDEIAELPLRLQSKLLRVIQQKTFERIGSNETIKVDVRIIAAT
                     NRDIEKLVQNQEFRSDLYYRLNVLPIQLPPLRERLDDVPTLAEYFLKKYTRETNRGVM
                     HFSESAMQCMLSYSWPGNIRELENAVERAVLIAKGQIITADDLMIGSKPNIADEQFSG
                     KDLREAITIFKKNYIQSALEQNRWNQTETARNLKIQRTYLSRLIKELNILQNKE"
     gene            complement(37269..39678)
                     /gene="rpsA"
                     /locus_tag="PLS88_00420"
                     /pseudo
     CDS             complement(37269..39678)
                     /gene="rpsA"
                     /locus_tag="PLS88_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
     gene            complement(39681..40433)
                     /locus_tag="PLS88_00425"
     CDS             complement(39681..40433)
                     /locus_tag="PLS88_00425"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOU05855.1"
                     /translation="MSEPKEKSIRLHAYLASLGIASRRSCEEMIREGRVTVDGVKASI
                     GQKIVCNERIEIDGEPIIVTQRKRLRYILLNKPKGYLSSLSDPYGRPCAIDLISGNVN
                     ERIYNIGRLDQWSSGLLLFTNDGDLASKLMHPSGQIEKEYRILTDVPIPENFASSFMR
                     GIIIDGIRYRARSVLVTGPNSAVIILIEGKNREIRRVLEYFDLHAKLLERIRFGPICD
                     PNLAPGQWRELTENEVQALKAFSDLNQKSKGK"
     gene            complement(40430..40987)
                     /gene="scpB"
                     /locus_tag="PLS88_00430"
     CDS             complement(40430..40987)
                     /gene="scpB"
                     /locus_tag="PLS88_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOU05856.1"
                     /translation="MNEQLDLEAALISAILFLENEPLSEEKLARIAGFTLEQTSLALQ
                     RICSEFDQPVHGFAPIKKETGWIFAPKLVFWEQLKDHYGKKNEMRLSRAAMETLAIIA
                     YSQPITRAEIEAIRGVNVDAMIRLLLEKNLIAEIGKRDVPGKPMQYGTTQEFLRCFGL
                     NSIEDLPKLDEVENTRFSRLETPEG"
     gene            complement(40971..41768)
                     /locus_tag="PLS88_00435"
     CDS             complement(40971..41768)
                     /locus_tag="PLS88_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HOU05857.1"
                     /translation="MTKEFGDDSIQEKNAFISNGRTFCLSDFEGPLDLLLYLIIKNEM
                     NINDIRISSITEQFLQCLDSDPSADLDEISEFYQMAATLLYIKSRSLIPHTEDEAEEL
                     DDPRRNLIDQLIEYHRFKKLSELMEQREMEVEWFVERKVAQRTLPFADNPDIDPWIPA
                     DSWDLLRIFASMIRNFSSERIIDLYEEVSINEKIALIYELLDKKGNFHFDDLISRNTS
                     AMDLACAFLAVLDSTKNKLISVRQHKLFGDILIIPYSAVEPSNERTT"
     gene            complement(41765..42277)
                     /gene="folK"
                     /locus_tag="PLS88_00440"
     CDS             complement(41765..42277)
                     /gene="folK"
                     /locus_tag="PLS88_00440"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOU05858.1"
                     /translation="MEKVWLGLGSNKGDSSSILDSAIQELGGFLYELRRSGLWRSRAR
                     YYNDQQDFLNLAVSGFTNLTPRELLIQIHRIEANHGRDRSKEIKKGPRTLDIDILIYD
                     QKIFAEPDLIIPHPGLAERKFALLPLLELDYELIHPGLGISLRELAACLPPQGIYPVN
                     NNRYDWPYSE"
     gene            complement(42315..43484)
                     /gene="recA"
                     /locus_tag="PLS88_00445"
     CDS             complement(42315..43484)
                     /gene="recA"
                     /locus_tag="PLS88_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOU05859.1"
                     /translation="MRGKDDPNPEIQKEREEKLRALEAARLQIEKQFGQGSLMKLGAH
                     TIAQGIDVISSGSILLDEALGIGGYPRGRIIEIYGPESSGKTTLALHAIAEAQKKGGI
                     AAFIDAEHALDPIYARNLGVDINELYISQPDNGEQALDIAESLIRSGAVDIIVIDSVA
                     ALTPQAEIEGEMGDSHVGLQARLMSQALRKLAGTLSKSKTILVFINQIRMKIGVMFGN
                     PETTTGGNALKFYSSVRIEVRRIDTIEGGQDEEAIGNRVRVKVVKNKVAPPFRKAELE
                     IIFGKGISWSASLLDAGVKCNVIEKKGAWYTWSGEKIGQGRENAKAYLEQNAEAAKKI
                     ETQVREILFSPDKSKTTEAIISQANPSGTNDSFNTAANIENSENNSSKIRTEDLF"
     gene            complement(43641..44963)
                     /locus_tag="PLS88_00450"
     CDS             complement(43641..44963)
                     /locus_tag="PLS88_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU05860.1"
                     /translation="MHQIKLSRLKPFSFIGFRMLLGRPAEAREKGVHKARHGIFLGAI
                     LGIGISIVPLYIVIFVSNGMIKGITERYLETKTSHLQMSIPFDLSEENRSQRIETISA
                     LPGVVFASLEIDGLGLAASAKSSTSAQIRGLEESITLDKGYRQFIEVDVGEPFPQKEN
                     EVVLGRYLAKALGVGLGETVSLMTLRETEGAEILPKLSIFRVAGIVSSGYRELDANWF
                     IIHFQVAQRLLNSDMAYAYIGIKTKDPYGPDLDKIMNNIKNLYPEALVRPWRDIEQGL
                     FKSFSSTRSLLIFIMAIASIVAAVNLSSVLTTFVIEHRTEIAVLRSFGISRKQTMLIF
                     LLGGTATGIIGCILGSAVGIAISLCINHIILAIQTLINFFTRLLNPSSYVITLLNPDY
                     YLERIPISMNWSYIILILCVGVLVSAIVSLIPAFRSSSIAPAELIRHE"
     gene            complement(44951..45634)
                     /locus_tag="PLS88_00455"
     CDS             complement(44951..45634)
                     /locus_tag="PLS88_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU05861.1"
                     /translation="MNKPIIECRGLTKTFASSAEVLCILDQANFEMGKGEICTILGPS
                     GSGKSTFLAILGSLERFDSGDVYVDGYDLSALSEKSLSMFRRSVVGFVFQFHFLLNDF
                     TALENVALPSYVAGIQRHEAWKKARKLLDMVGLGKRLDHYPSQLSGGERQRVAVARAL
                     INSPSIVLADEPTGNLDASSAKELRSLLQNLPEATGATLLIVTHDPDLAAIGGSSYLL
                     KGGRFECIK"
     gene            complement(45627..46910)
                     /locus_tag="PLS88_00460"
     CDS             complement(45627..46910)
                     /locus_tag="PLS88_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU05862.1"
                     /translation="MRFEPLLRIAVRWSITGKKSAHSSGLKFASLGIAVGVIALIVVL
                     SVMNGLQQQYIESLLETTSFHIRARVKEGDLDIFTNNLRQNRLVRSVIPFMETNLLVQ
                     GQEGRQNVLRVMWIDSEDLVLDFGYCRALNISPSAAAKSLEEGIILGSEAAHILGVSE
                     GSMLTLRGAYVDAEEGIQQYSERVKVSNLFRSGYYELDAALAIMPRQKAKNLENFPAS
                     TILGIKLINAENANSFASMLEHENGILDIESWQEYNRSFFSALHTEKILMFLLVGIIF
                     IVVAINIHYSMRRSIARKSRDLAIMAAIGMNQHSIATIFVLEGILVGLGGAIFGIAIG
                     IPIAQHVDKIINGAIGLIEAFISTLYHIGLVKGVPDLRVFSPSIFYIEGIPSRIFFSD
                     IALIACFAIIFPVIAVYLAYRRFRNASPLEVLRSE"
     gene            complement(46973..47839)
                     /gene="ftsY"
                     /locus_tag="PLS88_00465"
     CDS             complement(46973..47839)
                     /gene="ftsY"
                     /locus_tag="PLS88_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOU05863.1"
                     /translation="MNFKDKIKALFHRSQLSEETFEELEDLLIEGDIGASIAFEVVDT
                     LKNVCRKEGISTPEEARGKLKEILRPFIKIADFDFDDKRLNIVLILGVNGVGKTTSCA
                     KLARWAENNGIEQPIIFAACDTFRAAAIEQLKIHGQRLGARVIAQQHGSDPGAVLWDA
                     IDAARKNNARLVIADTAGRMHTRADLLRELEKLDRVVSQKAGDVSYQRLLVLDATTGQ
                     NAMRQAEIFNSSVKIDGVIMAKYDSASKGGMIIAVSKQFGLPTVFLGTGEKYGDLEPF
                     DIDRYLDDFVGD"
     gene            complement(47865..48773)
                     /locus_tag="PLS88_00470"
     CDS             complement(47865..48773)
                     /locus_tag="PLS88_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05864.1"
                     /translation="MKTYKNLLLIFLFLLIDTYIFAQASPSQSIGSIALVFPLQISNI
                     QDLDPLGKESVDSMMVLSFVHDKLSMSQYDTNNTVSPESRSLSRVMEETLRSRTTVEK
                     MTAIDIETLKNWNWSLIIQDKSALFLESSLQKADAVLFMMYTIEDSNMQLGSFLLKKT
                     GESYAFLSNSFSVFSLLDAARETVDSFIKESALIAKKSSLAYSELAIDKIDLPSVDPW
                     ENRDRYKKAQTAFETSIGGVVISLSVSFVAVGFWQIYREAAYRNSAFNNAMTISAIAA
                     GTSAAVTAAFLTVSIWNAGIMLGASH"
     gene            complement(48760..49891)
                     /gene="prfB"
                     /locus_tag="PLS88_00475"
     CDS             complement(join(48760..49821,49823..49891))
                     /gene="prfB"
                     /locus_tag="PLS88_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702248.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOU05865.1"
                     /translation="MLEDIAKPIEDLKEDIFEIWGRLDAPSIEQKIYDLEAQTNIPDF
                     WSEQTKAKKTLSEIKTLRDRLETWQELHNDILTLEEMYELAHQEDQQDMEADLQASLE
                     DIKTRFDRAVVIELLSGEADRNDAYLTIHAGSGGTEACDWASMLYRMYVRWAERHDFK
                     VETLDFLEAEGGIKSVTIQISGEYVYGYLKGETGVHRLVRISPFDANARRHTSFASVS
                     VMPVLDESIEIDIRPEDIRIDTYRAGGAGGQKVNKTDSAVRITHLATGIVVTCQNERS
                     QYKNKDVAMSILKSRLYEYYKAERDRETAKFAGEKKEIAWGSQIRSYVFQPYTMVKDH
                     RNKFSVGNVQNVMDGDLDPFLEAYLHWQWKGGTAVEEEDEDI"
     gene            complement(49906..50958)
                     /locus_tag="PLS88_00480"
     CDS             complement(49906..50958)
                     /locus_tag="PLS88_00480"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOU05866.1"
                     /translation="MHVATSAIEKKHFGTLSSGEEVSLFILKAGSFQASFTDWGATWV
                     SFIMPGKEGQKDDILLGFSMLSGYVGKHPYFGSTIGRFANRISGAQFTLNGIVYKLWK
                     NDNCNHLHGGRKGFDKHLWNHELSAFSGEPAIRFSRMSPDREEGYPGTLYVEVIASLS
                     SSGELRLHYYAETDEVTPISLTNHAYFNLAGEGKGTILDHELEMQASMYLPVDEYLIP
                     KGEICPVDGTALDFRFTKRIGRDIADVGNGYDFCYVIDKDYLPLKPFAFVSEPSTGRN
                     LSVATTLPAVQFYTGNNLAAITGKSGSIYDKYAGFCLETEMYPDSPNQQNFPSCFLFP
                     GKPWEHETVYRFDIQY"
     gene            complement(51036..51857)
                     /locus_tag="PLS88_00485"
     CDS             complement(51036..51857)
                     /locus_tag="PLS88_00485"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU05867.1"
                     /translation="MASVLDNKRVAFVTGATSGLGLAISRSLLDIGYIVYGAGRRQQP
                     PDLPQDFIYVPTDVALDESVNKSRDFVLSQESHIDLLVCAAGFGISGSIEDTSIDLAK
                     AQFDVNFFGTARVVCAFLPSMRQHGRGRIIIVGSIAGKIGMPFQAYYSASKFALEGFV
                     ESLRYEVRRFNIEACIVEPGDFKTGFTGSRQKIMTPESPYYDQFERVIGVQEHDEING
                     LDPAKAGKKIAGLAVSKHLPTRLTIGPFFERFAVWIRRLIPDAWFEVFYRMYYRL"
     gene            complement(51868..52386)
                     /locus_tag="PLS88_00490"
     CDS             complement(51868..52386)
                     /locus_tag="PLS88_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOU05868.1"
                     /translation="MGIIAISGKSGCGNSTVLHLVAEKLGYEPVNYTFRSMAADMGIS
                     FEELHKKAQESLEYDRKLDEHQVQLARKGNTVIGSRLAIWMLPEAELKVYLKASHATR
                     VKRIHAREGGDIEAIDRFTKERDAKDRGRYLALYNIDIDDISSAHLVIDTERWSAAQV
                     ADIIVYAYHLIH"
     gene            complement(52394..53260)
                     /locus_tag="PLS88_00495"
     CDS             complement(52394..53260)
                     /locus_tag="PLS88_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOU05869.1"
                     /translation="MKSMTGFAHSPILAKDFQGTLTLKSYNNRFLDISIFLPPYAYRI
                     EPLIRDYLGSRILRGKIECTLKLKKIELSLEDIELQFQAATLLSEQLRKLAKACNIEK
                     DLSLDLITKFPGIVDMNIEIDENNFWPILMPILEETWQEFEESRIREGTATEENIRGQ
                     LKRLKSKLNEIELQADSLERIVHEQIVTRFKELLSENYDESRVLQEVAVQLVRLTINE
                     EIGRLRAHIDAFESIARQPVCGKKLDFLCQEMNREINTIGSKSMLVPVSHAVVEMKDA
                     LENIREQLRNVE"
     gene            complement(53286..54827)
                     /gene="murC"
                     /locus_tag="PLS88_00500"
     CDS             complement(53286..54827)
                     /gene="murC"
                     /locus_tag="PLS88_00500"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOU05870.1"
                     /translation="MTQFPEKLEGLHVHLVGAKGTGMTALAEILKKRGAILTGSDVAD
                     VFYTDSILHSLGVELFENFDKNNLPLSTDIVIHSAAYSREGNPELKAATYRGIPIFSY
                     PEVLGALSLHSRSAGIAGVNGKTTTAALSGILIKALGLPAMVLAGSAISNFDDRCTLV
                     QGETYFIAETCEYRKHFLNFKPTWIVLTSVESDHQDYFPTYESIRDAFVEYVLSLPLN
                     GVLIFCADQKGAVEVADLVLQKRKDIKYVEYGFLAHGKYRIRDFQTKAGVNTFILGEN
                     SFPIRIHLPGKHLVQDAVAAIALVEQIYETEKSKTFEESEWNSIVEALATFRGSKRRS
                     EIIGEYEKILIMDDYGHHPTAIKTTIEGIKKFWPHRRLVVDFMSHTYTRTIALQDDFV
                     SSLDNADSILLHKIYASAREQPVSGFSGKTLYEKLCEHRPELFPIDLEKDNREKMITC
                     YKNKTREDKKGFIAYADEPLEALNPLTNLLQPGDIFLTLGAGDNWKLAKALAERLDKK
                     PATSK"
     gene            complement(54912..56828)
                     /locus_tag="PLS88_00505"
     CDS             complement(54912..56828)
                     /locus_tag="PLS88_00505"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1925 domain-containing protein"
                     /protein_id="HOU05871.1"
                     /translation="MQSFKVVIGMYNHLPYNASGALYEQTYQGAWRPFLSGLYKFLSI
                     KSVIYFSGAIFPWLEENHPEYMYLLVEMVRRGQVELLGGGLYNPLSPLISTQDMTGQV
                     ESLSAFIRKTTGKRPSGAWLYEYSWSSSIPSILQNSKIQYTFLPAEYFSYEQGSREFC
                     MPIASEDHRKVITIFPTFESRDSENSFVPYENTLLDLQESYPECKTFIIMADGNEIST
                     SWEKSGLESPDVLFERTFAWFQKNCLEYDTLTPIQLHKTTKPTRIIYFSQCYSQRFKD
                     YCKKTINRLPTKNQGYIQISKQSVLDHLLVHNLYQKLNYVSTMIALFRGDKSRKKASI
                     DDVWKAQCGDLFWIGPTGGILVPEARLGAFSSLIEAEKTIRQSRFHSYLSFDDLNFDG
                     LKEAIFQSSVYNCYLQSEFASVSEFDSIKTETNYACGWNDDQCSTGCFKDYISTEGSF
                     ESNIIAIEHWNMVENPKEESSVLFRREFSNRSYGHFLTLVCRKTYRFRNDFFSIDYEL
                     SNKNTEACQFRFCTKSEIIATPVFEDHRIELLHHRESKTLDLKSQLSFEMVDGIGLSN
                     LRKAERVLIRSDSPFSLFAKSNFLQKPTVSPQVLNVPSDFLMFEGFSINIGWDLSIPP
                     EGTVFFSLSVHLEH"
     gene            56912..57976
                     /locus_tag="PLS88_00510"
     CDS             56912..57976
                     /locus_tag="PLS88_00510"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU05872.1"
                     /translation="MIEARTAVDLRLTKHYGQFVGKLLTITLSRDDDGRRADRIVRKV
                     LKDTALSTIYRLFRQGDIRTSNSPIKPNDRLKEGDIVEIRLPEKIWEKGHSQTIIKSA
                     SLKKKHSQGSYDEIPSILWQNSHLIAFHKPRGMLVHDGNTNLDAYVHSFLHDLLEPSV
                     SFSPGPLHRLDRNTSGIIVFSKTRIGADLFSTAMRNRRIHKFYIAIVEGRIDEPILLK
                     DSILRDKKRRLSVIDKNEGREAILRAFPLIQSKLFSLLLVDLITGLTHQIRVQLASHG
                     YPLAGDSKYGGSKTPEINSYFLHSCCLGFVQPLFEDMPLTIFDPLPNDFGLAISYLFN
                     MNKREIDTLISHRIDQSLAI"
     gene            58011..59000
                     /gene="hflK"
                     /locus_tag="PLS88_00515"
     CDS             58011..59000
                     /gene="hflK"
                     /locus_tag="PLS88_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOU05873.1"
                     /translation="MADTPNPKKMWKIKLKWPLVITIIIALCVIFFLLTSFVIVDQTE
                     KAVITTFGKYTKTLGAGLHYKLPFGIQKAHLVKTQVIQTETFGFRTVRPGIVTQYSSE
                     KFPNESTMLTGDLNIVDVEWIIQYRVTDPEAWLFNVMDKEKTIRDTSQTVINMLVGDR
                     TILGVIGPERQNIQEAAVALMNEFFSKYGLGITVTQVQLQNIVPPEGVQAAFEDVNKA
                     IQDMNRLINEGREAYNAEIPKVQGQADQILEVAQGYASERVNVAKGDVSRFNSVYDEY
                     RKAPEITRKRLYYEMMEEIFKDQENIDLIDKSLQNVLPIKNLAQTPSSQEASR"
     gene            58997..59980
                     /gene="hflC"
                     /locus_tag="PLS88_00520"
     CDS             58997..59980
                     /gene="hflC"
                     /locus_tag="PLS88_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOU05874.1"
                     /translation="MKKTSIIITIVVALGILFIIIFGPLFVLNEGEQAVVVRFGKIVA
                     FHTSAGLKWRSPFVDTVMKYPKKLMSWDGEPQRIPTKENQFIYVDTTARWRIVDPIKF
                     YESVNTLDGAFSKLSDVIDSAVRTVISSNYLREAVRSSDEILSESQVESFQTGDIENN
                     TTLQQLAITQTQYEKIEKGRLVLAEEMKTLVSEIVPSFGIEVVDILPRQIKYSDELSE
                     SVYQRMIKERNQIAQAFRAYGEGKKAEWLGKLENEKRTILSSAYAKGESIKGKADAEA
                     SKIYADSFGRDPSFFEFWKAIESYRKTMPAFNKTLSTGMDYFKYLYSPTGR"
     gene            complement(59996..60715)
                     /locus_tag="PLS88_00525"
     CDS             complement(59996..60715)
                     /locus_tag="PLS88_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU05875.1"
                     /translation="MEHLFLALADIHGNIDALKQILKANPNIEGILVAGDLTNFGKEK
                     EAQNVLLAFSSITSKAALYFLAGNCDTIEARRYFEKQPGYIEQRCFPLLSEKDIWIIG
                     SGGGLIHTGLTPFEIREEELENGLNHAYKCAIQGYRNIIDSSELTPDLIVLTHTPPKD
                     TFADIRHSRHIGSIAFQRFLYEHEPLLWICGHIHEGKSIHIENRTLVVNPGPAAHGSY
                     ALILLKRYPKGWKAAAELRSI"
     gene            complement(60723..61952)
                     /gene="tyrS"
                     /locus_tag="PLS88_00530"
     CDS             complement(60723..61952)
                     /gene="tyrS"
                     /locus_tag="PLS88_00530"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOU05876.1"
                     /translation="MNRALEILKERGFVQQCSDLEGLSNLMDEGPITFYIGTDPTGPS
                     LHIGHMVPFFAMRHLRDAGHRGIALLGGGTARIGDPSGKTEMRKLISYEEIDANTERF
                     IDQLDRFIHFDGVNALTANNKDWLSNLNYIEFLRDIGRHFSVNRMLSFESYRMRLETG
                     LSFIEFNYQLLQSYDYLQLYKLHGCRLQIGGDDQWGNIVAGIELIRRVEGAECYGLTF
                     PLVTTSDGKKMGKTEKGALFLDPEITSPYEFFQYFRNVPDSDVERFLLMFTFLPIDEC
                     RYLGSLKDKALNEAKERLAWEVTSLIHGEEEANKALSAAKAAFRGEGDASQLPTIEVE
                     ISRLDSGIGVLDLFVEAGLAPSKSEARRLVKQSGASVNGRKIENEMQILTSRDLENNA
                     LMLRAGKKRVARVVPKS"
BASE COUNT        18545 a        12698 c        12407 g        18484 t
ORIGIN      
        1 cataggctat ttctttctgg tttgcaggaa catctacttc cattatatgt ccaagaagac
       61 gttttttctc atcatatttg tttgctgtca ccattaaacc tcgcgaaaca gttagccttt
      121 cattgccatc gtagaatttt atattaggaa agcgctcgct gatctttttc ttcaaatcaa
      181 gcttgatagc tggatcgact tcttttatgt cggatagcat cgaatagact ctctcgacaa
      241 cttgttgagg agcttcttga aggaattcct ctaaatcctt ttctttgaag agaatatttt
      301 gtacaagctt attgatcttc cggttttctg tcgtatcttt atggctatcg atttccttga
      361 atgttatatc tagtatatgt aaaagggtaa gaataatttt ttctcgcaca atgccaagtt
      421 ccttcatcca aggcttgtct tttaaatctt tagcaagcca tatgaaagct tctcgcatat
      481 ccttataatt ctctacgata tacatagcca ttcgcttgag tttttcttca tggccattag
      541 actcaagtgc atcaagcatt tttacagatc tggcataagg caaaagctga atatagatgt
      601 ctggccagct tggaataaaa agcttaatgt tctgaatgaa ctggttcttg agctccgtat
      661 ctttcagtcc aagatagact gcgacaatat tatccttgat ctgatcaaaa aggtcgacaa
      721 atcgcattgt cacatattgt tgcattcttg gatatcgtcc ggacagctct tttaagagta
      781 ggtaagaacc gacgacaaat tcgtttacct gtgtcaattt caggaatgaa acaaaataag
      841 acagcatttc ttcaaaataa tcggtgtcga cttcctcgct catttctaga taatctctaa
      901 aatactggac cctagaaaaa aagtttttct ccgccttaaa ctgcgcatag attttttctt
      961 cgaaggatag aggcctgtcg cgcacgataa aagtagaaat atcgtcctgg gcatttccaa
     1021 acataggatc tgtcttgagt atttctttgg cacgagtact ccatgaattc cactcagata
     1081 tagaaagaac agaaggcaca agctcagctt tgatgcgttt catatcagcg cgattatcga
     1141 agcttttgat tatgatttta attgtccagg ctgggtctgc ctttactttt tctcttaatt
     1201 tttctcgagg ccaggttgct tttagaaccc atatatgatc cttggaaaga gtaatgaggc
     1261 tatcgaaagc cattttcagc gacatgtgat gattgcgcga ttttgcaaaa tcaataacaa
     1321 cctcatcgtt ggtgatatta gaaattcttc caatattcca tgtcctatgg aagacataat
     1381 ttcctctatc aaaggcaata cgtttttcga agtcggcaat tgcctcatgg agagagcgat
     1441 aggattgggt gaggttcgaa agctttacac attcatcgaa cattgaatgt ccagagtaga
     1501 tatttgagta gcactcgagg aattctttgc gtaaggcagg attgcgctcg tcatattcca
     1561 cgataagctt gagaatgctc aatgcggtat cccatttttc attcttctta taccaatcat
     1621 ataaatccat taaaattgtg gcagcttttt ctggcgaaac ctgctttgca atttttttct
     1681 gtgcatggat aaaaaagtca taatcctcag gcgaaagttg aataagttta tgccataatt
     1741 cttttatatt cgaaaagagt cctttattaa tatagcgaat aagagctttc ttatagaaat
     1801 ctatagcttc ttctgttaag ccattctttt ccttccattc tgcaataagt tgtgctaatt
     1861 cagcttcttc ggtatcgatt cgtacaagac tttcccatac tgcatatcgc tgctcaatct
     1921 tattctctgc agtatagcat tctgctaatc gaactagggc aatacggttt tctccaaaat
     1981 ctaatattcg gttgcagata tattcgacaa tattactttt cttgctttct gcaaataaat
     2041 ccatcaactg aacaagataa gattcatcag ctggctggcg catgatattt agcattccag
     2101 cgacatataa tgcaataacg ctattctttg tttgagatag gtgttccacg caaatcttga
     2161 tgatatcatc agccccattc tgggccatta tttcctcaat aaagcgatct agttctttca
     2221 ggttggcgac agagtagttc gccataaggg ttcttgtcca tttctcttca ttcaacatga
     2281 gctgtagttt ttcacttaat ggagactgat ccattctatt ctcctctctt cgttattcta
     2341 tgtatgattc ataaattgat tgatcgataa tcttgatctt gagcaacact gaatgcaatt
     2401 ctttagcatc ctctcgggcc tttagatgat ccgccatcca aaaatattta tttagaaggc
     2461 ggggaacaag gctcgatcct tgctgaagtg attctcttaa ttgaatctca agttgacgtg
     2521 cagaggccga aattcgattg aattcagcgc tgctcaattc tcttttaata ttgaatacac
     2581 cccatatttc tgcataaact gggatccatt cagcaatatc atctccagaa taccctagtt
     2641 cttttatatt ttcaatgatc ttaaagatta attcagactc caaaaaatca atttctatag
     2701 cttgtggatc aataaaaaaa gcttctctga ataaggcttt agaaagacgt gcttcgcctg
     2761 acatagcata tacatcggcc aattcagcaa gaatgcttgc atcgtttcgt ctaccttggg
     2821 ctgccctttc aagatgaata agagctgtct ctatatctcc cgtagctttc ctggctctgc
     2881 ctaaccttag atctagcaat ggcccaaggg aatctttttc ttcatctgga atcgcaaggt
     2941 actgatcaag agcaagccca aatatgaatc tcctataaac atagcgagca gtatcgaagg
     3001 agtcccctat atgagaaaga aataacatga attttttcca ggagctacag agaaaatctc
     3061 cacgctccaa aagattccct gtttcaggca tttgcgagat aagatccttc cagaaatgaa
     3121 gacacttcat tgcaaataga acttctgggt gttcaaaatc tgctttcaga gcattctcaa
     3181 ggaagaaatc tgcactttgc atatctgcat tctttaggct ttcataagcc agatcaagat
     3241 tttcttcggc tgtggttgct tcttccttga gacgaggcag atgggccatt agaatatttt
     3301 tctccttaga ctgctattta gatagtatcc gagccttgaa tttttcgtca atggctattc
     3361 tatttgctat ttccaattaa cttaacataa aactcaagca aggagtatgg aaaaacagtt
     3421 cgatacaagc tatcatatat aacattatga aagaaccaat aattgcgccc tcgattctat
     3481 cagctgattt ctatgacatt ggttcagctg ttaggtatat agaagagagc ggatgtcctt
     3541 ggcttcactt ggatattatg gatggacatt ttgtgccacc tatcacattc gggcataaaa
     3601 tgatagcaga tattcgagca aagacaagac tattcctgga tgctcatctt atgatagaag
     3661 aacctgagcg ccacatcaat gcttacatag aaagtggcgc agacgcaatt acttttcatt
     3721 ctgaagcttg tgtgcattca catcgtctaa ttcaaagcat attacatgct ggcattaagg
     3781 caggaatatc gatagtacct tcgactccag tctctagtat aattccactt cttgaatcag
     3841 ttgaccttgt acttgtaatg actgtaaatc ccggctatgg ggggcagaag cttatacctt
     3901 tctgtttaca aaaggtagaa gaactaagaa gaataagaga acaagaaggc cttaatttct
     3961 tgatcgctgt tgatggtggt ataaatgtga aattgatccc cgacctcgtg attcagcagc
     4021 cagatgtgtt agttattggg agcgcttttt ttagtgcaca agataagaaa cagtttgtta
     4081 caaattttct agatgcttgg ataagcccaa tcagaaatat tgattgactt agatgtgagc
     4141 tttccaagat atataaacaa aaaatattgt accgggaaga tagaagtatg aaaaagttac
     4201 aaatgaatat gcttatgatt gttatgctta ttgtttcatc tttcacttat gctcaaacta
     4261 ccaaaacatc agacgaattc tcttatgctt taggactttt taagaatggc gattttgagc
     4321 gtgctggaat tgcttttgca gaaatatatc agacagcatc agacatagag atcttggctg
     4381 acagctgctt tatggctgct ttatcatatt ttcaagcaac tgactttcct acctcttata
     4441 tattttgcga caaattcatg caatcttttc cttctcatcc aaatatgcct gatatacaat
     4501 tcctacaagg tcgaattctg ttcaaaatag ggctatatga ggatgctata ttggtattcg
     4561 atacttttat gaataaatac ccagatagtc cgttttcctc ttctgtattg ttctggaaag
     4621 gagaaagcta ttatcagata ggtgattttg ctcaagcttt atccattttt aataaagtca
     4681 aagaaacttg gcctaacacc gaagcgactt ctcttaccgc atggagaatt accgtaatgg
     4741 ctctagaatc tagagaagcc aaatatgctc gcctcgctga attcgagtct cggagaaaca
     4801 tgtatagaga tattcaacag atgcaggaag atgtatatgg agaacaacga cttctaagag
     4861 attacctgtg gtcaaaagca attagtaggt attctgcaac gccaaatata gaaatccaaa
     4921 aagcaataat agataacatt tcaaggaatg atagactcaa taggcttctt gacgcgaaag
     4981 agagggcttt gaagctggtt atattaaaaa tagaaaatta ccttaaggat atagcaccat
     5041 gaaaaaatcg atattcttct tcattttatc gtttatattt acaggtgctt tatttgctat
     5101 tgatatacgt agcggccttg tccgaattgc tactgatgat cagacattgc agccgatgct
     5161 ttatcgcctt gtaaatattt ctggaaaatc gaaatatgaa cctttatggt ttgatggtga
     5221 tccaaggacg agttttatta tattaaatat cgacaaccgt atatatagaa tgggagcttc
     5281 aaaggaatat aaaactagtc agcgtcaaat aaagaatggt atcgaaatcg aatatagatc
     5341 ggttacaaat agattgactc agagagtgct tttttataaa agcccctatt ctcgggtaac
     5401 agatggtttt attattgaaa tagaaataga aaactataca actcgagaga tgaaggtatt
     5461 gataaaagaa gtatgtgata ctgttctcgg cgaaaaagga ggctataatt tcgctacaag
     5521 ttcgaatccc agggttacgg acgaaatgat tatcacatcg gattctgatg aaaagtatgt
     5581 tatttctcca ggacaaacat cttccattgc tttgcttcta gatgaagagg caaagccaga
     5641 catggttgtt attgcaaact ggaagcgact gagcgattca aaattctatt acgactctga
     5701 gcttatgaaa ggctttacgc aatctccttt ctctatcaac gattctgcac ttggtcttta
     5761 ttggaatgaa agaattattc caccaaaggg tagcataaaa gtggcgagta cttggcttac
     5821 cggtggacca ggaaacgaaa tgatcgactg gctatcacaa aattatcctt tccctaatca
     5881 agaattgcaa caagcctttg agaaccctcc tgtggttttt gaagaacagc ctgctgcaaa
     5941 tgttccgaaa ctagatattg aagccattaa agagcttata tctgagatag atagggctat
     6001 aggcaatatc gataatgtca gcgataaaga aatacaggac attttgtcaa ggctaaattg
     6061 tcttgaacag agcgaagagt ttgccgcttc tcagtcgaat taaggaaagg gaagcttata
     6121 tgagagctgt aatacagcgt gtatctgaag cttttgtgac atataaaaac actaaagagg
     6181 ataaattctg tggaagaatt actaaaggaa tactaatcta tctcggaatt gcagccgatg
     6241 acacagatgg cgatgctgaa taccttgcag ataagatagc ccatctacgt atttttatgg
     6301 atagcaatga aaaaatgaat ctttcgattc ttgatctggg ctatgaggca cttgtaattt
     6361 cgcaatttac cttgcacgct gatgcccgaa aaggcagacg accttcctat tcacaagctg
     6421 ccggtccgga aaaagctttg attctatatg atttattttg cacaaagttg atggaacagg
     6481 gagtctttgt ccagactggt aaattccaag agatcatgcg ggttcattcg attaatgatg
     6541 ggcctataac aattttgctc gattcgaaaa aggcttttta atatttatca agactaggag
     6601 taaaagaata ggagcgctag aatggcgctc ctatcttatt tggaacctgg aagatactac
     6661 tagattttgg ctatctattt ttagaaccac gcggtctgcc acgagggcgc tttgccttag
     6721 aggaggttgc ttttgcaggt ctaccaggtt ttcttgcagt tttatgagca gcttgagcat
     6781 cttcgatcac tgcctgtgct gcagcgagtc tagaaacagg aactctgaaa ggggaagaag
     6841 aaacatagtt catacctact cgatgacaga attttacaga ctctggatct cctccgtgtt
     6901 caccacagat gcctaccttg agtctaggcc tcgatgtgcg gcctttttct atacccattc
     6961 gtatgagcat ccccacgcca tcctgatcga tattctgaaa tgggtccgct ctgaaaattg
     7021 ctcccttctt ctcgtcgata tagtctggca gaaatttacc ggcatcatct cgagataatc
     7081 caagagtcat ttgagtcaaa tcattcgtcc caaaggaaaa aaattcagct acttttgcta
     7141 ttctatctgc aagcaaagcg gctcgcggaa cttcgatcat cgtacctatc atatacttta
     7201 aaggtacttc tgatgtagct ataattttag ccgctatctc ccgtgctctt gactcaagaa
     7261 tttttagctc tttgtcgtcc ataaccaagg ggatcataat ttccggaaga accttaatgc
     7321 cattcttggt catattgcag gcagcggtga ttatagctgt aacctgcata tctagaattt
     7381 cggggtaagt gattgtcaaa cggcagcctc tgtgacctag cattggattt agttcatgca
     7441 actgttctac tcgttgaact accttttcaa aaggaacacc aattgcatcg gctagctcac
     7501 gctggccttc ctcatcgtgt ggaacaaatt catgaagagg tgggtcgatt aatcggattg
     7561 ttacaggcag gccatccatc gccttaaata taccctcgaa gtctttagtc tgaaatggga
     7621 gaagtttagc tagggctttt ttacggcttt ctgtatcatc cgcaatgatc atttcacgaa
     7681 ttgcgaggat gcgttcttct gaatcaaaga acatatgctc agtccggcat agtcctatgc
     7741 cttcggctcc taattcccta gcttttttag catcataagg agtgtcggca tttgtgcgga
     7801 ccttaagcgt tctagtccgg tctacccaag acataagagt ggtgtaggct ttaggtggtt
     7861 cgggctttac cagcttgagt tgacctagat acacattgcc agtattgcca tctagagtga
     7921 tataatctcc ctctttgaca atcgtatttc caacagcaaa ctggccattt tcatagtcta
     7981 tttcgagttt ttcacagcct acaatacagc acttgcccca tcctcgtgca acgacagcgg
     8041 catggcttgt cttgccacca gtagctgtca aaatgccttg ggctgcatgc attccgccta
     8101 cgtcttcagg actggtctct ttgcgaacaa gaatgatttt ttcgcctttt tcttcccatt
     8161 cttcggctac ctttgcattg aataccacct tgcctacggc ggctcctgga actgcatcga
     8221 taccttgcac aagaaaagat ttcttaagtg ctgcagggtt tgatttatct atcatagggt
     8281 agaagattcc ctctatatcg gaggctttaa tacgaagtat tgcttcttct ttggtaatca
     8341 gcttttcttt tacaaaatcg actgcaatcc ggaaggccgc aatggggctg cgttttcctg
     8401 tgcggcactg aagcatatag agcttacctt cttccacagt gaattcaaga tcttgcatgt
     8461 ctctataatg cctttcgagt tttcttcgga tgtcgactag ctgtctatat acttttggat
     8521 cacgtttttc gagctccgcg agctttattg gtgtcctaat cccagcaaca acatcttccc
     8581 cctgagcatt gatgagataa tcaccataaa agatattctc accagtattt ggatctctag
     8641 tgaaacatac accggtacca gaattttcac ctttgttgcc aaaaaccatt tgaacgatat
     8701 tgacagcagt tccttttatt ccaactagtt tctctacttt gcggtaggtc actgcttttt
     8761 ctgccatcca agaatcgaag accgcattga tggctccttt tagctgttcc catggatcct
     8821 gtggaaaatc tgtcccgata tgttcgcgat atatcttctt gaaatatttg ataagctcac
     8881 taagctcttc ttcgtttaca tcggtatcgc tcactttttg cccagtaggt attcctaagc
     8941 gcagtcttgt ttttgtctct ttgatttctt caaaggcttt gtcgaatacc tccctatcga
     9001 ttcccatagc tatagagcca tacatcataa taaagcgcct atatgcatct aaagcgaagc
     9061 gtctatttcc ggtcttagaa gccaggccct caaccgaagc atcagtgaga ccaagattca
     9121 aaatagtctc catcatgcca ggcatcgaaa caggtgcgcc agatcgaaca gagacaagta
     9181 atggatcact cggatcgcca agagtctttt ttgtcaactt ttcaagattc ttgatatgct
     9241 tttggatctc tgcttcaagt ccttcaggcc attttttccc attcttataa taaagctcac
     9301 atacttccgt tgtgattgta aaaccagcag gaactggaag tccaatttta gtcatctcgt
     9361 gaaggcctgc acccttgccg ccgagaagct ctttcattgt gccatctcct tcggcaaggc
     9421 ccgttccaaa tgcatacacg aatttttttc ttggcatgag gtttctcctc caatagatag
     9481 aaaaaaagct gacgaataaa taattcgctg cggtggaaaa ctaatcgaaa cagctaaatt
     9541 agtcaagata tgtataaata acgttattaa ttatcttaat tatattatct aaatgatata
     9601 aaactttact ctataccatt aggttatcat catctccaaa agatatcgat ttggggcgaa
     9661 gtgctcacag tgagaagatc tgccattgcg cattctagga ttgtcttttt tccagaggta
     9721 gttttgctaa taaaactgcc atcttgaata aagacttccg gaggagattt agcagtttca
     9781 attgtcagag aaaatcgata agatgcgaac tgttcagaaa tatacttttt catatcggga
     9841 tcaagagggg gaatctcatc tccaaaagag aattttaaag aatagatacc attttctttg
     9901 ttgaatctgg caaagctgtt agtaggatct agatatttta ccagattttc aggagaatca
     9961 aaatctaaag taaaagaaat aatcatattg ttagcgttct tcgtctttga gtatgatttt
    10021 aaggaaatac cttctgtgtc ttgtaatcct cgctcgatat cttcgcgaga taaggcaaag
    10081 ggtagagaag gagtagattc ttctgatcca aaatccacaa agtctttggc aatttgatat
    10141 tcaacggaga atgttcccgc acctttagag tcgatcttag tcgaagcttc aatccctatg
    10201 caagaaatga aatagaaagg cagagagatt attaaagcca agctgagcaa tatcttccag
    10261 agatttctca ttagattagt cctctcatcc gtatatctca taatgcatct gcacttcata
    10321 gagctcaact ggataatggt cctctatgaa tgttacagca tctctcagca ctttttctcc
    10381 gtattgtgca gaattagata caagtgcagc tcctatttct gcaaaggcaa gtgaatcaag
    10441 caagtcgatt tcagcgcatg aaagtctgta tctgtgctga agtctatctt ttattcctga
    10501 aaccactctt cttttatctt tgattgtaga tgtttcattt aatctgagca ccaatcgcaa
    10561 cattgataca accatttgat ataatattat cacttgagct tgtccttgac agcccctttc
    10621 tacagggata ttgtccaagc catgaaaaga agactcataa catcggctct tccctacgtt
    10681 aataatgttc ctcatcttgg caatctgatt caggtattat ctgcagatgt atttgctaga
    10741 ttctgtagac ttcgtggata tgagactctc tatgtctgtg gtaccgatga atatggcact
    10801 gcgactgaga caaaagctct cgaggaaaaa gtaagtccgc gtgagctctg tgataggttt
    10861 tatgaaattc atcgcgatat ctatgaatgg tttgaaatat cttttgatac ttttggtaga
    10921 acatcgactc cagagcacac agagatcaca caatccatat ttttcgatct agacaaaaac
    10981 ggttacatct caagccaatc catagatcaa ctctattgcg atacctgtgg acgttttctt
    11041 gctgatcggt atgtaagagg aacctgtccc tattgtggtt atgaaggagc ccgtggtgac
    11101 caatgtgaga tgtgcgggaa actgctcgac ccaatggaac ttctgagccc taaatgcata
    11161 agttgcggtg gtactccaag accacgctcg accaaacatc tatatctgga tctccctgct
    11221 atactcccta agctagaagc ctggatgaaa gaagcaagtg taagaggttt ttgggcaaat
    11281 aatgcaatcc agatgacgca ggcatggata cgggatgggc tgcatccacg cgctattact
    11341 agggatctaa agtgggggat tcccgtgccc aaagagggat tcaaagataa ggtcttctat
    11401 gtctggttcg atgcgcctat cggctatatt tcaatgacag caagattagc aaaggaaaag
    11461 gggttcgact ggaagagttg gtggcagaag cccgatgaag tagagctttt tcagttcatt
    11521 ggtaaagata atattccatt ccatactgtc attttccctt ctagcctttt gggtactgga
    11581 aagaactgga ccatgcttca tcatatgtca agtacagaat atctcaatta tgaagctggt
    11641 aagttttcca aatccaaagg tattggcgtt ttcggaaatg atgctatgaa tactggaatt
    11701 cctgcagata tatggcgttt ctatatattt tggaatagac ctgagacttc agattatacg
    11761 ttcacatgga ctgatttcca agaaaaggta aatggagagc ttataggaaa tctcggaaat
    11821 cttgtaaata gaacgcttac atttatttcg cggtattaca gtggaattat ccccgagggg
    11881 acccctgata tggctttctg gaaggaggtt gccgatcgcg aacaagaggc ttcagaactt
    11941 ttagagcggg ctgatctccg agatgcgttt agaactatct tttccatagc agatttggca
    12001 aataagcgtt tccagtatga agagccttgg aaagctagaa tagacaatcc tcaaaaagcc
    12061 agctcactta tgcgtgatct atgctatgtg ctgaaagacc tagcaataat gacttatcct
    12121 tatatgccaa gcacggctaa aagactcgca tcttttttag gaatagaaat aggacaaggg
    12181 aaacttggtt gggctgatct tggtttaaaa ggagagtttc aaagaataaa aaaacacgag
    12241 gttcttttta ataagctcga agatgagctg atagcttccc ttcgtgtaca atattcaggc
    12301 tcccaaaaag aaagagaaga aaaagaagaa caaaatcgta aaaatgaggg aaagctacca
    12361 gcttctatag aaaaaaatca gaacaatcga tttgatcaaa taatagacct acgcgttgct
    12421 cggattataa agattgagcg acatcctaaa gccgacaaac tatatataga gaccctagac
    12481 gatggcagtg gttcagaaag ggtaataatc tcaggactag tgcctttcta tagagaagat
    12541 gaactacttg gcaaaaatat tgtccttgtt aacaacttaa agccagcaaa attgcgtgga
    12601 attgaaagtg ccggcatgct tctcgcagct tcgaccaaaa gcctagaggg aaatgaaatc
    12661 gtcgaagtat tgactgctcc aaatgcaaag cctggcgaaa gagtgttact agaaggaagt
    12721 agtattgaaa atctagaaga gactatatcc aaagagaaca tcgatattga aacattttta
    12781 acaatccctt tatatgcaaa agatggctat gcttggtcag aaggtcaacg ccttatggtt
    12841 cgtggcgagc ccataaggct cgaaaaagtt atggatggaa aaattggctg agtaaagaac
    12901 caataaccga gattttagtg cagagtgcta tggatgttct accgaaaata gaaataagcc
    12961 ccattctatc agcaatggat tgtatgcctg aatgccatga gcagggcttc ttacaatcca
    13021 aattttgggg acttttcaag gctcgcaccg gatggcaagc ctattcatgt acatattctt
    13081 tctatgataa ggatttgagt ggtaggatcc ttgttttaaa aaggaaattt gcaaaacttt
    13141 tttcttttct ttatgttcct tttggagcag gagaattgag ttctatctca aagcgatgga
    13201 atgctctatc ggcgcttgga cgagctctgg gtgaatcttt tggcggtacc gatgttttca
    13261 tacgttttga tttgccatgg gaacgagata aagcattagc aaaaaataaa gaagaagaag
    13321 aaaagcttga tatagagagc actcttggat ttcgtattcc tggcttatat aaaggcaccg
    13381 atgttcaggt gccagataca gttatcttag acttgcaggg cgaagaagaa caaattcttg
    13441 ctagaatgaa gcctaaatgg cgctataata taagactatc acaaaaaaaa gccatttccg
    13501 tagaagatga aggtatttct agtttgccat tatttatgtc tttatataaa gagacagcca
    13561 agcgagatag aatagctatt catccagaga gctactataa gactttattt gaaacaagct
    13621 ctgaaatcgc agaagcagct cgagcaaatt ctattgctac gataacgcct catctgtctc
    13681 tatatgttgc tcggcataaa gaagaggtac ttgcaagtat tattgtcctg cattttggag
    13741 acacttcaac ctatctctac ggtgcatctt cgagcaagaa acgcaatcta atgccaacct
    13801 atgcattgca atggcaagcc attctagatg caaagagatc tggggcaaaa aaatatgatt
    13861 tctttggtat accgcctgat gataaagatg attctcatcc tatggctgga ctctatctct
    13921 ttaagacagg atttggaggt aatataatac atcgctatgg agcttatgat atcagtctta
    13981 gacctcttct ttattctgtc ttcagaacag cagaaaaaac gcgcagcctt tggcataaaa
    14041 agataaaaaa aactttgaat aaaaagacag ttagactatc tgatgagact atgaaaaggt
    14101 taacaataaa gaaggtacaa aaaagtcagg aatccgaaga ggatgcttct ttaagctccg
    14161 attcagaaaa cacgaaacct tcgaatccat aaagttgcga aagaggagat ttccacatat
    14221 cagaaggaag gcctgctttc cagcaaagct gctctaggaa agtctcttta tcccatccaa
    14281 attctattgg aacttgtgga agcaatattc ctgatcttcc atgagcagag atgagaagac
    14341 cgtgtttccc tatcacaatt tcctctggag agattgggta taagggagag agcagagtga
    14401 tttcaatctc gatcaagggt aattcttcct ttttcaaggg cggaaaacgc gggtcttcga
    14461 atgcagcggc aagagccatt tgttttatag tttttgttaa atcagcatca ctctgtattc
    14521 tgccgataca acccctaagc gatccatttt ttctcaatgt gacaaaagct ccacatttta
    14581 gagatttaga taggagcgga ggctcgactt cttcttcgcc ttttaacgac gcattaattg
    14641 tgaggcgtgc ataattcaat agtttctttt gatcttctag agatattgta aattccataa
    14701 ttcacctctt aaaatctatt gctccatagc ctacaactag ctcatcacag tactctctat
    14761 gtttcgaaga attatcataa tccagtaatt ctgcccttgc ttttggaaat gcaactctga
    14821 aagctcgaat aactgcttct ccgcaaaaag aattacagca agattctgct tcaacatgaa
    14881 attcagcaat aggtctagaa agagaatcga cgaattcctt agaaagcgaa tcacattttt
    14941 ctggagaatc gcttactgca aggtttgaag atagtacaaa gataacctga ttcccaatat
    15001 aattgcttat ttcttcaaat aatcttgtgg cagtctcaat agtcgaggga ttcttacccg
    15061 atataataca aggcaatatt gggacccctg ggaaaaaacg agatgcaaat ggtaattgaa
    15121 gctctatcga atgctcttcc aaatgtggta gatcgttttt ctttaacatc gttacctttg
    15181 taaggagata atctctaagg ttcgaatcaa tattcaagga agttcctgga atatcgaatt
    15241 tagtagattc aggcagaaaa acaccttctt cataaggcag atgagaaggc cccataatcg
    15301 caataataga aggatccgca cccagaattg caccccacga ttccatgctg acttttccgc
    15361 tatagagaaa acttccatgg ggagcaataa tggcaaatgc tggacaaggc attggctttg
    15421 cgtacatact aagattgtcc atttcggaca ttagcatatc tggatcagat ggatagaaaa
    15481 ttcccgatag caaagggctg cgcaccagtt tttcatcact catttaaatg ctctaatcta
    15541 taagtatatt gctaaacctt gaaaatgcaa gaaatgtgga ttgtaaattt ttaagggaac
    15601 atcgattgct tacagattat catcatatta ttatttcaac aaatggaaac agtttctcaa
    15661 gcacttctat atgaagaaaa attgcttcga aatcgccttc agagagtcga gagcgaatgc
    15721 gcatcgagac ctaagggtag cattgtacta aaacgccgat ataaccaagt atatgcatat
    15781 ctgcaatgga gggatggtaa tagagtctgc agcagatatc taggaaagcc cgattcctgg
    15841 caatgcagat caatacaagc aaagattacg gaaagacgaa aatacctgga agaggcaaaa
    15901 gaaataagaa aaaagctgga agctacgcag catcttctag aggaagttag aaaatttagt
    15961 tgaaaataca gcataaatag gatacaataa tcacaatgaa agatattttt ataatacacg
    16021 aagaagagta tgatactgct ccagactgta catcagctgc gccgacaata gttaaaatcc
    16081 taggagagca cactgatttt actgatggtc ttgtgctttc agcttctctt tcctttgaag
    16141 tccgagtcgc tatatctttc agaaaagaca attcgctgcg tttttatgca gctgatttta
    16201 atgaacgtaa acgcgcaaac gttgccacat tcaagtacaa aagagaagat cgctgggcta
    16261 atcatgtaaa aagcgtgtgc gactattttt ggcgcgtatt caaccttgat ccccgtggtc
    16321 tcaatgtgac tattcaaagc aatgtgccgc ttggacttgg tctaggtatt agtagcgcga
    16381 taaacatggc aactgcactt atcttcaaaa cgctatacga tctggacctc aaactagaag
    16441 aattggcttt tcatgcctgg aaagctcaga gcttgttctt tgagaaagaa ttacctataa
    16501 caaattatct tgcaattact gcgcccgcag ggagaagttt ttcaatcata gacttgcatg
    16561 ggaaaaagcg ccgcggagtg caatttttgc ctgaaccttg ggaaatgctt ctaaccgatt
    16621 ccaaagtgcc gcgatcttct gtggaagcag agctggaaca gcgaatcgca gattgcaaag
    16681 catgtatttc agttcttgtt ccatatcata accgttctat ccgcgatata gcaccacaag
    16741 aactcgatga actgctggga atagtgcctg aacgtaagcg tagacgatgt cttcatgtgc
    16801 tagaagaagt aagaagagta gttgaagcgg aagagagtct ttcacgtcag gactatgtag
    16861 gctttggtcg aattgtaaac aaatcacatg cttctttaag aagtctttac gaaatttcat
    16921 gtccggaaat agactggctt accaagagag cgcttgagtt agatgatgtt ctatgttcta
    16981 gacttgttgg ccaagggttt ggcgggagca cgcttacaat tctcaaaaat gaaaaaaagc
    17041 aagtctatcg caataagcta gaagactatg agagaatatt tggtttcaaa cccataatcc
    17101 acgaagtctc gacaggcgca ggtcttagaa ttctagagca ttgattctaa gctaccaatc
    17161 ttattctgaa aacggagatt acatgagaat cttattaaca aatgatgatg gcatacaatc
    17221 ggatggcata tttgctctca tcgatacaat tactgcaatg aaagatgcca atgggaaaag
    17281 tttacaccaa gtatgggtag tcgcgcctga gcatgaacgg tctggcgtga gccatgctat
    17341 gacgttgaaa aagccagcta agttgcgcag acttggagat ttccgctata gctgctctgg
    17401 aacaccggta gattgcataa taattgcagg acttgctatt ctccatgaaa aaccggatct
    17461 agttatatca gggataaata gaggaccgaa tcttggcaca gatataattt attctggaac
    17521 ttgcggcgcg gcacgtcagg ctgcgttgca aggaatcccg gcaattgctg tatcatgtgc
    17581 agcctattgc gataatcttg aatatgaagg actaacatct ttcctgtcag gcaatctaga
    17641 agagcttata gccttgtgga agcctgattc attcatcaat attaatggtc cttctacaaa
    17701 atctagagat ctaaaggcag tatggactgt cccaggaaag aataagtaca ttgacaacct
    17761 taagtgcttt gacggattgg atggatatac ttattgtttt ctcactgaag gaggggaaga
    17821 gcgaacgcct gataatctct cggatcatca tgtaatttct cagggccata ttgcaataag
    17881 cttaattgaa gttcatcctc gcgcatttca tgagtcatct ctaaatggga aattcgtttt
    17941 ttaacattta gattcagcta ttttcctgct attattaata tctttaaagg ccttgaccaa
    18001 atcattgtct ttcgtatata gtgcggttcg ttatgcacgc catcttagct cagttgggag
    18061 agcgcgtgac ttgtaatctc gaggtccccg gttcgattcc gggagatggc tcgagggaca
    18121 tatagtccat caatggggag attcccgagt ggtcaaaggg ggcagactgt aaatctgttg
    18181 gctgtagcct tcgaaggttc aaatccttct ctccccacaa ggggttgtcc tgagaaatgg
    18241 acagcccctt tttgtttgtt ttgcacaaag gctttttttg attatctctc ttggccgcct
    18301 acaggatggt ggtactatga atcctgaaat gccaataaaa aagatctatt tcgaatgtat
    18361 gcgttgcaat gcttgctgtg gcggtcaacc tggttatgtg tggctctcca agaaagatct
    18421 tatggcttta agtgagtatt ttggtctttc tatacaggaa tttgcattgg ctttctgtag
    18481 aatggtcgat ataggcatag aaattacatt aagtctaaaa gaaaaacaga actatgactg
    18541 cattttttta gaaaattatg gatgcagcgt ttatccggtt aggccggttc aatgcaagac
    18601 atatcctttt tgggaaacaa tactagaaaa tgagcagagt tggatcgaag aggctaaata
    18661 ttgccctgga attggaaaag gccctctgat atcctcaaat acgattttag atgcttttct
    18721 tgagagacga cagaatactc ctctatcgat cgatgaaatt acagctctga gagctttaga
    18781 gaataaatgg gataatcaat gaaaaaatat catatcagat ttattatgca gaatcttttg
    18841 attttatttt tgattttgtc aatggccata ataatcttga ttataacccc tcccttcaga
    18901 agtaaaagtg tagaagtaaa gataaaaagt ggatccagcc ttagagatgt tgctcaagag
    18961 cttcataatt ctcatgtcat accttcctcg aggatatttg agctctatgt acgtgccatt
    19021 ggatctgagc gcagcataaa atctggaacc tatcgcttta tgccttttga gtcgcctatc
    19081 gcagcagcaa gtaaattgat aagaggatat tacataactc aaaaagttgt tattccagaa
    19141 ggtaggaccg cgcgccaaat tgctacgatc ttggaatcga acaatgtaat aaatgcagaa
    19201 gcttttttgg aagcagttaa tgatacagag cttgctaaat cattgggcat tcccgcctca
    19261 agctgtgaag gctatctttt cccagataca tattttttta atccgcaatg cgactcgcga
    19321 gaactcgtat cacaaatggt cgctggtttt aatgatgcaa tagaaagaat tgaaggaact
    19381 aaaacaaagc tattaaatcc tcagtccctt catcaaaggg tgatacttgc ctctattata
    19441 gagagagaat atcgattacc cgaagatgca cccctcattg cctcagtgtt ttttaaccga
    19501 cttcaaagga atatgcccct tcaatcttgt gcaactgtag tatatgtaat gacagaacat
    19561 ctagggaaag aagagccctc agttgtgcat tatggagacc taaaaatcca agatccatac
    19621 aatacttata taaaccgagg gctgcctcct gggccaatct ccaatccagg agaaatcagt
    19681 ttaagagcag cattatttcc ccctcagaca gattatttgt attttagatt agatgatgtt
    19741 aataccggga aacaccgctt ctcccatacc ttggaagaac ataattatgt agaaataatt
    19801 cccaaggggc tatagaaaat aatggcgaga atatcaccac agattagaca ggaaattctc
    19861 gataagaccg atttcttatc agtatatcag gagcatgtcc gtcttcagaa aaaaggagct
    19921 tctttttggg ggctttgccc ttttcattcg gaaaagacgc cgtccttttc tgtaagtccc
    19981 gacaccggcc ttttctattg ctttgggtgt cataagggtg gctcaattat tcaatttata
    20041 atggatatag atagattgag ctatacagag gcaatgcaag aattagcaca gcgatgtggt
    20101 gtccagattc gtttcgaaga gtctgaagaa ttaggagaaa ccgaaaagga acgtcaggct
    20161 cttcttgagc taaacgaaag gttatccaag acttttcact ggtttttgat gaatcatgat
    20221 tttggtcgtc cagccttgga gatccttcat aaaagaggtc tatctgatga attgattgag
    20281 aaattcagca taggctatgc ccctgcaaat agacaatggc tttattcatt tctcaaagca
    20341 aaggggtata ccgatgattt tttgaaacac agtggccttt tttcaaagaa agatctggca
    20401 tatcctctct ttgcagatcg aataatgttt cccattggag atgccaaggg tagaataatc
    20461 gcttttggtg gcagagccat gaacgaagat ggtccaaagt atattaacag ccctgataca
    20521 gcagtgtttc ggaaacaaga gactctattc gccttctcac aagcggttga ttctataaag
    20581 aacagcgata ctgcaattat atgcgaagga tacatggatg cactttcatt tcatgctgct
    20641 ggattgacaa atgcagttgc gcctctggga acggcattca caagtcaaca agccatgatt
    20701 atccgccgaa aatcatcgaa tataatacta tgctttgata cggacgaagc aggattgcat
    20761 gctgctgaac gagcttgttc gatagctact gcgactggcc tagaaactcg agtgcttctt
    20821 ctagaagagg gcaaagatgc ttcagagata ctggaaaaga agggagcagg ggagttgacg
    20881 agaatggcta aaaatactat aaacgctgga tatttcctgc tcacacgtgc caaccagctt
    20941 ttcgatatat cgacaatgga gggaaaagcg aaagcagtct cttttctgtt ccccttcctt
    21001 gacgctctcg attcagatgt taagcgacaa gaatacatca aggaagtagg aagaatcatt
    21061 ggagtgagca gtcatgcggt tgctatggat tatgcaaaag cgaaagtatc cgggatgcgc
    21121 cgtaccagag atacttacgg catttctaat ttaccatatg gcagctcgaa ttcagctaga
    21181 acttccgacc ttctatttat ggcagcaatc attttgtcgg aaaatccatt ccagtttata
    21241 gatgagaact tggatataga aaatttcgac gatccaaggg caaaagatct atttttggct
    21301 cttcaagaag cgcagaagga aggaattttg gacatagaag ggattttatc tcgttgtact
    21361 gatgaggctg ctgcccgctt tgttagagaa gtcgatacat ctggcgaact gcaaggtgga
    21421 ctagaaaaaa ttataaagga tggatttgaa aagaaaaaga aggccgcttt ggagagaaag
    21481 cgcagagatc ttgtaacaca gcttcaatcg ctttcagtag atgcagatcc attgcaagaa
    21541 aagctgatcc ttgaagaaat aattaatatc gatggagaac ttaaagctac gggaggagat
    21601 attaatgaat gatatagagc tcgatcccgc aattgcaaag ctactcgaat atgcaaaaga
    21661 aaaaaagact ataagttttg atgagctttc tgatatgttg cctgaacaaa tactcaattc
    21721 ggaaaaaatg gaagctatca tcaaccttct tgtgaacaac gatgtaaaaa tagaagaaga
    21781 aattcagatt gaagaagaat ctcttcccaa actcacttcg attcccttaa aaagcaaaat
    21841 ggtctcaaat gacaaggaga tatcgagcga tgacccagtt aggctttatc tgagggatat
    21901 cggaagagaa aaccttctaa cagctgagca ggaagtcgag ctatctagga tgatggagca
    21961 aggcgagaat atcatcaaga acacgctacg aaaatccgga atgctcattc cagagttcca
    22021 tcaaatagta gttaaggcta ctcgaaaaga accaaaagat atttctttgc aaaaaaaaga
    22081 aaatacagag aaaattgcag aacggagaag gctaaatcaa ttctacaaag acctcatcga
    22141 tacaagtgct gcagatctta aggcttatat tgagcttaaa aagagtataa tcgcaaaagg
    22201 cggaaatatc tttgaagatc cacaattaaa agaaataaga gaacaactgc taccaattgt
    22261 aaacgagact gatattcatc ccgaagaaat aacagtcttt tcagaaaaat ttatcgtcgc
    22321 tgcaaaaaag ataatccgct atagaaaaga gcaagataga atacagggaa ttctccagat
    22381 ttcatcatct aaagacctga ggacccttgg aagaaatcta accataaaag aaaagcgcga
    22441 ggaaattgaa cagcgccttg gccttagtgc ggaagagatc aaagacaaaa ttcgacagct
    22501 ccaagttaat gagaaaaagc tgcaagatat cgagaataat ttcgagactc ctattgacaa
    22561 gatcacttct atggctcgag aagtgaatcg tggccgaaaa atgatgaaga gtgcaaagga
    22621 taggctaatc aaggcaaatc taagacttgt tgtctctata gctaagaaat acacaaatcg
    22681 tggaatgcag ttcttcgatc tggttcagga aggtaatatt ggtcttataa aggctgttga
    22741 aaaatttgaa tatcgcaagg gttataagtt ttccacctat gcaacctggt ggatacgcca
    22801 agcaattaca agatcgattt ctgatcaggc caggactata agagtgcctg tccatatgat
    22861 tgagcagata aacaaggttg ttagagaatc cagacagctt atgcaaaagc ttggccgtga
    22921 gcctacagat gaggagattg cagcgcaact tggatgggag atagaaagag taaaagcggt
    22981 aaaaaacgta gctcgcgaac ctgtttcgct cgacacaccc atcggagaag acgaagattc
    23041 gcttttgcag gattttatcg aagataaaga agtagagaat ccagctgtcc aaaccaatta
    23101 caacttgctt caggagcaac tgcgaatggt actctccaca ctccctaaac gtgagcaaga
    23161 agtattgaga atgcgttttg gactcgatga ggggtattca ttgacattgg aagaggttgg
    23221 tttgtatttt aatgttactc gtgagcgtat tcggcagatc gaggcaaaag ctctcaagaa
    23281 attgcgacaa ttcaaacgaa gtcagaagct tcgtgactat atagatcatt gatagaggtg
    23341 acccatgccc atgagaatgg atgaattatt tgagactctt aggaaatacc aaaagctgct
    23401 aagcgatcgt gtagaattgg aaaagcagct agaggctatg cctaaaacaa ttgaaataca
    23461 aaatcagatg ctttcgagag aaaaaaaatc ttttgttgat gtaaaggaac aatttacaac
    23521 tacagaaaca aatctcaagc agcttcgtca ggcacttgct gaagcagaaa tgaggcgcga
    23581 aaatgctgaa aggcagatgg acggcattca gactcagcgc gaatatgatg ccgtaaacaa
    23641 ggaaattcgt gaagcttcag aaaaagaaca ggatcttcga caccgaatcc aagaagaaga
    23701 acgtcgtttt gcagagatcg atgataagtt gcatcgtata gagatctctg tttctgctct
    23761 tgaatccgaa atcgagcaaa aacggacaga gatagagcag aatagcaaac aaataataga
    23821 tcaaatcgag aagcttcgga cagaggaaaa tagcctgact caagaacttg aagatgattt
    23881 gaagttcaaa ttcgaacgaa ttatacgaag caaatcaggc atgggcattg taccgatcca
    23941 tggtgtagta tgtaccggtt gccacatgat tctgcctgca aattttgtta atgaagtccg
    24001 acaagctgat aagatagtct tttgcccata ttgttcccga atcctttact atgaagagac
    24061 ggagattgaa ggatcgatcg aagagatact cactggttca cttgtggatt tgggagaaga
    24121 agatagcgaa gagacggaag aaactgagtc agaggatgaa tcagaggaca tttcaaattc
    24181 atcaaaaaaa ggatcttcta gaaaaagaaa aaatcaaagt gaaccactta ttgacaatga
    24241 ttttcttatc gataatatgg aagaataatt atattttagg cgggctgggt tgccgcaggc
    24301 attatgcttg aggaaagtcc gggctcggaa gggttcgatg ccggataatg tccggggcag
    24361 gaagagcctt tagttccgaa tgttcggggc ttaaggcttt atcttgtaca gaaagtacca
    24421 cagaaaatac accgctgcgt ttatacgcag taagggtgaa aaggtgaggt aagagctcac
    24481 cgcattcctg gcgacaggaa tggcatggca aacctcatcg cgagcaaggt caaacagcga
    24541 tggataaccc atccaagtcg ctataagcgg cagaatcgcg ggtagaccgc tagaatgtac
    24601 cagcaatgat acatcgagac agatggcaac catgccgaga gtgaaattag ctcaaagcat
    24661 acagaacccg gcttacaggc ccgccttttt ttggtatatt atttttcaat gttcgataga
    24721 ttttttagag gaaaagctcc tcgaacaaga aaaaaaaatc cttggctttt catagcaata
    24781 atattgattg gagtaggtgt attcctaggt ttttattttg ctattagcag caaagataca
    24841 aaaaatggca tatctcgatc agctttggca aaaacctgga attctggaaa taatatagaa
    24901 aagacattat ctgaagcaca gaaagccata gaaagctatc cctttgatga atattacctt
    24961 agtatttgcg gcatagcata ttattattca gctctcaatg ctcaggaaga ggagactcga
    25021 caacaagagt tggaaaaagc ggttattgct cttagaaaag cccttgctgt tgggttgcct
    25081 tcaaggatga agggacaagt ctattatata cttgcaaagg cctattatca aaaaggcgaa
    25141 ccatggttcg atcttgccga acgttatttt ttgcttgcaa aggaaagcgg aagcaaagag
    25201 aaagacattc cacaatactt agctatcgta tatgctggca gaaaagacta taatcgagct
    25261 gtttcttggt ttgaacaagc gcttgagaac aacacatctg acgcgctttt attgtcagct
    25321 gcaataagtt acatgaatac agaccagaaa gagaaggcaa gagacttgct tttacgctta
    25381 gagagttcgg catctgatgc aaagattagg ctcaaagcat cattattgct ggctcaggac
    25441 aacttcgact ctggcgatat tcaagccgca ctgaatagat atcagattgt actaaaggaa
    25501 gatccggtca atgtggacgc atggtatggt cttggcctta tatatgcaaa gttgaacgat
    25561 cagcttggtg ctcgagcagc ttttagaaag gttgtacaaa tcgaccctaa tcatgcggat
    25621 gcaagaaggc gattggctga aaaattatag aaagaggaga aaagcagtgg gtatccttga
    25681 catgttcagc cctgatattg gaatcgatct cgggacatgc aatacattga tctatatgaa
    25741 cggcaaagga attgtgctcg atgagccatc tgtagtagca gtggagaggg ggacaaatcg
    25801 tattgtagca gtaggaactg aagccaaaag catgctttcg agaacgccga ctgatatcat
    25861 tgcaaagcgc ccgctgcggg atggtgtaat tgccgatcca gatatgactg agaagatgat
    25921 caaatatttt attcaaagag ttttccccaa gaatcttttt ttcaaaccaa ggatagtggt
    25981 tggcgttcca acatgcatca caaaggttga ggaaaatgct gtcatagact ctacttacaa
    26041 agctggtgct agaatggtga aagtaatagc agaatcgctt gctgctgcaa taggtgcagg
    26101 cattccaata actgagcccg ctgggcatat gatttgcgac ataggtggtg gtactactga
    26161 aatctcagta atctcattga aaggcatggt ggtaacgaat gccattcgag ttggtggcga
    26221 tgaattcgat gaagctatag ccaaacatat tcgaagtatt cataatctga taataggcga
    26281 gcaaaccgcc gaaagactca aaattgaaat tggaaacgcc agtccagaaa gaacaataga
    26341 aaaaatagag ataaaaggta cagatgcaat cacggggctc cctcgccgct tcgaagtcga
    26401 ttctgtagaa atacgagaag cgcttatgga ttcagtaaat ctaatagttg aagaaataaa
    26461 gaaaacgctt gggaaaactc ctcctgagct agcagctgat attgtggaac gcggtatcgt
    26521 tctttcggga gggggagctc ttctaaaggg actaccaaaa ttgattgcaa aggaaactgg
    26581 cgttccagtg attctatcag agcaccctct tgaatgcgtt gcaattgggg ctggtaagta
    26641 tttcgagatt atacaaaaga atattggaag cagcagtgtt tacgagagtc taaatctctg
    26701 atattggcag gttaggcatg aagattggca gccaacaaac tcatggacct gctcgagtaa
    26761 tgcacaagaa agctggcatc tgtatgatgc tgtcaattat tgtgctattt atatcaacaa
    26821 agacgatagt caaagttcct agttttttca aaggttatgt aattggggga attcagaaaa
    26881 gtctatctgc aattggctca ttctttactg gaactgtcgg cgctgtatct gaacttcgaa
    26941 aaattgagaa agattatgaa gcattgctgg aaaagaccca ggattatgag ctaaaagtac
    27001 gagattacac cgcactgaga gaagaaaacc aacgcctcaa ggagctttta ggccttagta
    27061 cattgcctga tgcaaaaaaa atagctgccc atgtcatagc tagagatcca actaatatgt
    27121 attcctcact agtaatcgat aagggatata taaatggtat aaggaaatat atgcctgttg
    27181 tggcctacca aaacggtatc gagggtttag ttggtaaggt aatcgaagtc aaagcttcga
    27241 ctagcatact tcaacctttc tatgatcaac gtttttttgc agctgcaaga ttagcaaaaa
    27301 cccgtgctga aggaatgctg aatggccagg gctttcggga tgtcccactt aatttgcttt
    27361 acatccccaa gagtgagatg gagcaactca aacaaggaga tatcgtagtt accagcggct
    27421 tggacgagat tttcccccca gagataatta taggacgggt agagggttgg cgactcaatg
    27481 aattctccag ctcattagtt attagtgtac aacctgcagt tgacctcagt agaatagagt
    27541 atgtgttcgc aatagatatt caagctccct cttctcctgg taaagaggcg attcaatgaa
    27601 aagcatatta atatcaagca ttgttgcggc actcatgctt tttatacaat caacatggct
    27661 atcgcgcggc ataatcatgg gtattattcc caatttagct cttgatgtgc tcctctttac
    27721 ctctttcatc aacaaagaag ggcagggtat cattacttca ttcttagttg ggattgtagc
    27781 agatattctt agtgcctctc cacttggata ttatgccttt atttatacaa gttgtggcta
    27841 ctttgctacg ctcataagtt atgtgacgga gaaagacatt tttatcgttc ctttccttct
    27901 tggggcagta gcaacaataa ttatggccat attatctaag gtgatttcgc ttctgttttc
    27961 ggcaggcatt cattattacc aggtattcag tatggaattc ggtgttgaat tgatattgaa
    28021 cgggctattt gcaatactgc tgttcttttt gctttctttt atgagaccgt tgttcgagca
    28081 caatcccaag aaagctatgc catgagcatc atctttgcaa aaggacagat ataatggcca
    28141 tgctgcccaa aaaaatcact ttgcgcatac gcattttgaa agccctaata ataatgctcc
    28201 ttgttatcta ttcgataaat ctattctcta tgcaggtctt gagaaaagaa ttatttgtaa
    28261 gcgaagctca gcgcatttct atccaatcaa ctagaattcc tgctcccaga ggtgaagtat
    28321 atgatagatc gggtcgaaat cttctagcag gcaatataga agcttattcc gtttatatta
    28381 cgccttctga acttcctggt tccaaaaaat ctgaagttat taataagctc agtctacttt
    28441 taaacattcc tattgaggat atagagaaca aacttcctga ttcaagtcta tattcatatg
    28501 gtagaatagt aatagctaaa aatgcgagtc tggaagctgt tacgcagatt gcagctagaa
    28561 tcgatgaatt cccaggcatt tcatggagtt ccaagcctct tagaaattat gctgatctac
    28621 aatcgctttc taatattatt ggttatgtag gcacaatcac tcgagatgaa tacaagctgc
    28681 tatataataa gggctatata ttagaagatc ttactggaaa agcaggcata gaattggcat
    28741 atgaagatct tcttaaaggt aaggatggct ggatatctaa agctgtcgat gtctatggaa
    28801 aagatatttc gaacaatgct gcagaaatac aagaaccgat ccctgggaag agattgatac
    28861 tgagcataga cagcaaaatt caaaaaatcg cagaagaagc tcttggccct cgtcaggggt
    28921 ccgtaattgt acttaagcct gcgacaggtg aaattttggc aatggttact tatccttatt
    28981 atgattccag aatattcatg tcggataatg ttggtaaggc atacttagag cttctgaatg
    29041 atcccaataa gcccatgctc aatcgagcct atcagtcctc gtatccagta gcatctactt
    29101 tcaagacagt ccttacagct gctattctag aagaaaacgc gatttctcca gataaaaaaa
    29161 tctattgcag cggtgagtta atatatggtg gccgttcttg gagctgtcat attaaaaagc
    29221 ctgggcatgg agccctagat cttaagaatg ctcttgcgca atcatgtgat atttattttt
    29281 ggacagctgg tcgggactat cttgggattg aaaatattgt cagttatgca caagacttcg
    29341 gttatggaaa gcctactggc atcgatttgc cagcggaaaa cgttggtctt ttgccaacac
    29401 cgacatggaa agaagaaaaa tataacgaaa tctggacacc tggcgatacc atgaatctct
    29461 ctataggaca aggatatctg ttagcatctc ctcttcagct tgccaatatg atggcaatga
    29521 tcgtcaatga tggaatcatc tataagcctc atatcttgaa agaagtaaga gatccaacta
    29581 caagcgctct tataaatgaa atacagcccg aaattctaca ccaatcttca atttcatcaa
    29641 ggacattcga gactctgaaa tcatatctcc gcggtgttat tgtttatggt acagcaaaag
    29701 taccaataaa caccaaagtc gttcaagtcg ccggaaagac aggaacagcg gaagtgggtc
    29761 ttaaggatag atggcattca tggtttgtat cttttggacc ctacgatgca cctgctaaag
    29821 accaaatagt tgtagccaca atgatagaag cctcgaatcc ttgggaatgg tgggctcctt
    29881 atgctgctaa tgtgatatat caagcaatat atgctaatca aaatgcccat gacgcagcaa
    29941 aagctgttgg ggtacgtttg gagggttctt ccttgatagg gaaaagggaa tgaaatctca
    30001 aagtttcaaa agattgtttg gtaacataga ttattatctg cttttctcat gctttttact
    30061 ctgtgcattg ggaattgttg caatctattc atcaggaatc gatgcagaag gagtcactgt
    30121 ctctaatgaa tacttaaaac aaataatatg gtttggtatt ggagtctttt tgctgattgc
    30181 tgcagcagtt ttcgattttt caagatataa agatatggta tggatattat ttgcctttgc
    30241 aatattgcta ctaattataa cactgcttgc aggaaaagta gttaatggag ccagatcatg
    30301 gcttggaatt ggtggtatcg gtattcagcc agctgaattt acgaagatcg caacagtttt
    30361 aatgcttgca cgttacctag aatctgcgga atttgattca agctttaaga agttattgat
    30421 gactggcctt atcatgagta ttccattggc cttaattctg gcgcaacctg actttggttc
    30481 cgctcttgta ttcattccag cggctcttgt aatgcttacc ttggccgatg tggattttca
    30541 atatattcta tttggagttt tatcaatagt cggggttttc ctatttctaa gccttccttt
    30601 atatgcatca aatcacccaa acccagaaaa tatcttattc actgctataa caaataagaa
    30661 tgttttgata gctatactta tctttatact tgtaatatcg attatctctg gaatcggatg
    30721 gcttaaattc aagaagcgat attattactg gctctcatat agtatggcaa ttttggccat
    30781 ttcaacgata ggagcaatta tcgctcatag tatattaaag ccttatcaga taatgagatt
    30841 gcttgtattc atagatccaa gtatcgatcc caaaggctca ggttggaaca ttctccagag
    30901 cattactgct attggatctg gcggtttttg ggggaaagga ttcttgcaag gcactcatag
    30961 tcatgccagg tatattccac aacagagcac agatttcatt ttttctataa tagcagaaga
    31021 attcggtttt gtaggttgta tcgtactttt tattctatat ggaattctct tatttcgatg
    31081 tattgttctc atcgaaacat ccaaggatcg atttagccaa tatgttatcg gtgggctgtt
    31141 gggcatattt gcatttcact ttatgatcaa tatcggaatg gcaatgggaa taatgccagt
    31201 aactggaata cccctatatt ttgtctctta tggtggttca tctctctgga caggcttaat
    31261 atccattggc attctaactg gaatttctgc gcgcaggtat tcagcatgaa tacgaacttt
    31321 atagatcctc taagagatat tggagaagca ctttttgagg tacaaaagcc tgctcgctac
    31381 actggaggag agtgtagcgc tttttcatca atagaccctc tagattcccg actgagaatt
    31441 gcactttctt ttcctgattt atatgagatt ggaatgtcga acaatgctat tagaattata
    31501 tattcgatgc tcaatgaaca gaggagcgct ctcgtttgtg aacgggtctt ctcgcctgct
    31561 ccggattttg aacatttact tatggaaaaa caacttcctc tttacacgct tgaaagtgga
    31621 attcctctta acaaggtaga tgtcctggca ttcagtattg ggtatgagct tcttgctact
    31681 aatatccttg caataatgga cagaggctgg attcctctcg aaactaaaaa gagaggagaa
    31741 aaagatccaa tcataattgc tggaggacca gctattacaa atcccttgcc ttttgctgaa
    31801 tttttggatg cggtttggat tggtgaagct gaagaaggat tcacagagct tatgcttaga
    31861 ctagcaaagc taaaaagcaa tggagcaact cgaattgaaa agttaaaagc cttggcttta
    31921 cattcttcga tatggatatc tcccttttta caagagttag ggataccaac aaaaaaaaag
    31981 gtaaaacgag ctatctatca ggacttctat agaacaacct atacatccaa ctttccatat
    32041 ccagtaatca atcctgttca ttctcatggt tctgtcgaga taatgagggg ctgccctaat
    32101 ggttgtcgtt tttgccatgc cggttatttt taccgtcctc agcgatcgaa atttccttca
    32161 atcatcaaga aggaagtaga ggatttgatt ataaagaaag gatataccga aatcacactt
    32221 tcttctctgt cttcaggtga ttatcccgat attattggac tattcaatga ccttaccgca
    32281 ttatggtcga aatataatgt atcgtttcag cttccaagtt taaaggtaga tagtttcact
    32341 ttaccactta tagaaaaaat atctgaagta agaaaatcgg gtttgacctt cgcgatagaa
    32401 acacctcttg attcatggca atgcggtatt aataagcgtg ttccattgga aaaaattaaa
    32461 gcaatacttc atgaagcaaa acttaaaggg tttaaatcgg ccaaattcta tttcatgata
    32521 ggccttccag tagccgaaag gggtaatgga gaagaagaag cgattattaa ctacctaaat
    32581 aatatcgcaa gcattgaaca tattgcgatt catgtgaaca taggtacttt cattcccaaa
    32641 cctcacacac ctttcgaaag agaagaacaa ttatccgaat atgaatctct aaaaataata
    32701 aagaatatac gaaaaaattt aagatctact aaaaacattg aaataagtta tcattcgccg
    32761 tttttatcag ttcttgaagg cttgatttcc agaggtgacg aatctattgg cgaaattatt
    32821 ctatatgcct ataaaaaagg cgctagattg gatgcatggg aagagtatat cgatcccgat
    32881 atttggaata atgcgctgaa aaattacaat tcaaaaagag gggagggggc ttggcaaaga
    32941 ttcttagcaa gaagggatga aaatgaaaat cttccatgga atgagatttc atttaaaata
    33001 tcttcaaagt ggcttaaaaa tgaaagcaag aaagctaaaa gcaatcaatt aactttaatt
    33061 tgtaatgata attgtactaa tagatgcggt gtctgcgaca ataagaactc aatagtatct
    33121 tataatatac tatataaaga tataaaagac agcagcctcc caatttttag cgttgaagga
    33181 aaaaatataa gatctattaa caataatgat aatgtctcat cattagatat cgataaaagc
    33241 gatggcatta gtttcataaa ttgtgctgac attttaaata attatataga tttcaaatcg
    33301 actactgcaa taaaaatcat atgtgtttgg gagaaaaaag atatagcagt attatatcca
    33361 atgcatgatg tagcaaaggc tttttataga gcatttcaaa tatctggtct ctcaatagca
    33421 tatactcgcg gatacaatcc tatgcctaga atcgagcttt ctccaccttt gcctctagga
    33481 gttgagggag aaaatgaaat actaattgct tggattaata tatcgcaaga taatatttcg
    33541 cagatcaata gattattttc tttgcatcaa gaaaatattc tttatttgat caatttgaat
    33601 cttcccaaag ggctttatct taaaaatatt aaggtaggcg ttttccccca agaaaaaaga
    33661 aagacaatag gatctttact taaggctacg gcatggaaat atgaatttat aggcaagcct
    33721 tcttattcaa aggctgtaga tatattgaaa gaatattcag acttttttat accgataaat
    33781 gttacagaag ctgtagataa ttcatctgaa aatgagatag agttattaga aaaattttct
    33841 ggaagttctg gtaaaactat tagctttttt aagatactta aagaaaattc ttattctttt
    33901 tgtttccaga ataaaaatat tgaatcatct cgtaaatatt ttagcgctaa aagaataggt
    33961 tgttatggct acaacgaaag caatggctat gatttacttc aaaatatcct ttaaccaaat
    34021 aatgcatata aacttttgtt cggatcgaag ttgatcttag cattaataga ttttcgagtt
    34081 tattggtaga cagaatatac attatgcgaa caaactgata tcaatcgcaa taaatatttt
    34141 ggtactttga attctaagta ccaccaggaa tgtactctac tgacggctca aggatgactg
    34201 gatcgctata gacatttttc atcgtggaag gatcaaatcc ataggcaaaa gcaaagaata
    34261 ctatataaac tgagccacct ggcgtgacag aatttgttcc tgcgtagtct cgatcgccaa
    34321 ctttatattc tgaagaccga taaaaatcag cttttacaat atcagcacta ctcgcagtat
    34381 cacttctgtt tcgcacaata gaatcaatca cagtatttga gccttcatcc gtaagcgtcc
    34441 atgttccttg cgtattcata ttaaggttat atttattgct agatacctta attaatgact
    34501 cctttttaat taatggacta atatcaagag aatattgacc agatgcagat attctatgct
    34561 tcaaagggta atattgataa taattagccg tctgaattac attggagctt gtcatgtttc
    34621 tgatgttata atagctctgt tctgcttcat ttttatcatc aaaaactcga taatatattt
    34681 cgtaaccgag aaatattgaa gagatagttg gctcgtcgat attttccttg ttatgataaa
    34741 tgactaacac tccgttggct gaagagaaat catctggagg aaataaatac tcagccgttg
    34801 gaagcccaca agatatcatt ataataaaaa aacatacaat ggatatctca aataaagtgg
    34861 ctatgaggtg agacataagt ctaaagcttc tctttcccct ctcccctctc atccttttta
    34921 gccaccttat ctattaagat aacgtcctta tctaggattt tcttagtcag caagaccctg
    34981 tgctttcaag tatatcacgc gattcttacc taacatcgcc cattcggaat caccaaatga
    35041 tgatataagt tgatcataga cttcgacagc gcccttataa tcgcctttag tttcctttat
    35101 gcgtccgaca ttaaagaatg cgtgtggtgc tgcaggatta tcttttttat aatccttggt
    35161 aatcagaatg taatgttcga gagcagcgtc gatgttacct gcttcctctg cggcaacagc
    35221 agcgagttct aaggcaattg gcgccacata ggatttctta tttctcttat aagcttctaa
    35281 agcgactttc tcggcttcag catattcttt tttttgactc aaaatagctg cttttctaat
    35341 taaagcccgc tgagcggcaa ttgtttttgg ccatttattt gctatagagt ccaactctgc
    35401 catcagagaa gtctctattt ctgctctttt ttgttcatca gtctcctgcg accactgcat
    35461 cgttttttga tcagccaact ccattgctct gctcgacgca gaagcgatat ttccagacac
    35521 cgcagtataa attccgatag ctacaataat taatacgatc gcaattccga gaccaattat
    35581 aatctttcta ttcttgctta taaagtcaag aaatttttct gaagagcttt ttttctcttc
    35641 ttttttgatt atatctcttt ctttcttttg ttccttatct ttttctggtt gtacttgctg
    35701 aatttcttct tgattcacac attactcctt attttgcaga atattgagct ctttgatcaa
    35761 tcttgacaaa taagtcctct ggatcttcaa attgcgagcc gtctctgttt gattccagcg
    35821 attttgttcc agagccgatt gtatataatt cttcttaaag attgtaattg cctctctgag
    35881 atctttcccg ctaaactgct catccgcaat gtttggcttt gagcctatca tcagatcatc
    35941 ggctgttatt atttgtcctt ttgcgataag tacagcccgc tccaccgcat tttccagctc
    36001 tcttatattt cctggccaac tgtagctaag catgcattgc attgccgatt cggaaaaatg
    36061 catgactcct ctgtttgttt cccttgtata ctttttaagg aaatactcgg ctaaggttgg
    36121 tacatcatcg agacgttctc tcaagggcgg taattgtata ggcaatacat tcaagcgata
    36181 ataaagatcg cttcgaaatt cctgattctg tactaatttt tctatgtctc tattcgtggc
    36241 ggctattatg cgaacatcta ctttaatggt ctcatttgag cctattctct caaatgtctt
    36301 ttgttgaatt actcgcaaaa gctttgattg aagcctcaaa ggcagttcgg caatctcatc
    36361 gagaaaaata gttcctccat tggcggcttc gaatctccct gccctattct ctatagcatc
    36421 agtaaatgct ccttttacat gaccaaaaag ctcactctct aaaaggcttt ctggcaatgc
    36481 tgcacaattg acacgtataa aaggcttctc gcttcgtttt gaaaggagat gcaactgctc
    36541 ggctataagt tcttttccaa ctccgctctc tccaagaatc aaaactgaag catcgctcgg
    36601 agcaactcgc ttgactagct caagccgatc atgcatgacc ttagaagaaa aaataagggg
    36661 atgccatccc tcaccttcct gaaccttatg ctgaaggtag gtaagttctt gttctgtctt
    36721 ctcatactgt cttgcattat caaaggcaat gccagcttgt actgcaaaaa gctctaccca
    36781 ctcgaggtct tcactgataa aaaggccatg agaagccttg ttgatcagct ctatcacacc
    36841 ttctgtccta tcttttacct tgaggggaac cgctagaata gaattggtac tataagagat
    36901 ttctcttgaa accgttggtg aaaagcgagg atcattggct gcatcattca cgatgataga
    36961 acgtcggttc ttaaataccc agcctactat cccctctgtg gcatgaacag attttccctg
    37021 gagctcagca cctcttgggc caagtgcaat ttcgaatctc aggctctctg tggacgcatc
    37081 gaaaagagcg aggctcgccg cctcagcgcc cacaagtctc gctgctgatt caacgatagt
    37141 ctgtagcaga gaacgaaaat ctgtgtaact agaattaagt tgcgcgttga tatcgatcaa
    37201 cgtacggaat ctatccgaat ctattaccga aaccatttct gccattgtat aagactgaac
    37261 aaaagtgctc agtcgttttc actatccttt gcctttaaga ggtcacctag cgtatagcct
    37321 gcctctcctt catcttcctg aataaatcgg gaaatttcct cacgttcttg tttctggact
    37381 aaatccctaa tggaaagccc cagtttttgg cgatctggat tgagatcaat tacaactgcc
    37441 ttaatctgca tttcaggatg atattttttt aaggcttcgt cataagactc atttcgatcg
    37501 gataccaaat cctgcttctt aatcaagcct tcgatatttc ccggtacttt gacaaataca
    37561 ccaaagtcag ttacggaaga taccgttccc tcgacaatag aaccgggctt gtatgtcttg
    37621 gcaaaagaac gccaaggatc ttctgtcagc tgctttacac ctaaacggat atttctttcc
    37681 tctggattgc tctcaagcac aataacttct atttcatcac cttcatgtag aacagaagat
    37741 ggattcttta ttttttgtgt ccaagagtag tcgtctatat gaaggaaacc atcgattcct
    37801 tcttctaatt ctataaaagc tcctgcattt gtaaccttaa caacttttct ctttagccgt
    37861 gtacctactg gatagctttg atctattgtg tcccatggat tgggttgaac ctgcttaaga
    37921 cctagcgaaa ctttaccagc tggaatatcg tatccaagaa tcatgcagtc gacaatatcg
    37981 ccagctttta gcacatcctc aggccttcga actttcttca accatgaaaa ctcagagata
    38041 tgagcaagac cctcgatacc ttcttcaagc tctataaatg caccatagtc agtgagttta
    38101 gtaacctttc cctttacgat atcaccaaca tgatatttct cttcaaaact gttccatgga
    38161 tccggagtaa agtgttttag cgaaagattg atacgtttct cttctggatc taaacgaatg
    38221 actttaagtt caatttcctg tccttttcga acaaaatctt ttggacgtgt tacatgaccc
    38281 cagctcatat cgttgatatg cagaagaccg tcgaaccctc ctagatctat gaaagcacca
    38341 aagcttgtaa aactctttac aacacctcta acagagtcgc caatttgcgt gttttggaag
    38401 aaggattcgc gtttgcgttc tatttcttct tccataagtt ttcgcctgtt tagaatgatg
    38461 ttgatttttt tatctgagta caagcgctcg agatagaacc tacatcgcat tccaacaagc
    38521 tgttcacttt tttctacacg ttgtacatct gctttgctca aaggcagaaa acctgataat
    38581 ccatggccaa gatcgacctc gaatccgcct tttatttctt ttgtgatagt gccttcgact
    38641 ggttgatgct ctttgaaagc atttgctatc gatttccaaa agagtttctc atcggccttc
    38701 ttctttgaaa ttattatatc gcctgagtga gtctctttac gaattaagat taccttgacg
    38761 acatctcccg gcttaggagg aatatcgcca aattctgata catttagttt gccttctgat
    38821 ttgtacccta catctataaa aacagtgtcg ccagctattt ggacgacctt tccttcgact
    38881 aaatcgccct cctccagacc ctcaaacgat ttgaggtact gctcctgcaa ttgtgtttgg
    38941 atatcgtcct tggagggatc ttggacgtcc attcccactt ccatattatc tgccatgtgc
    39001 aatccttgcc cttagaattt tgtatacttt gtcataaact tgctctatgg tcaagtcaga
    39061 ggtatccaaa tatatagcat cttccgcgat tttaagagcg ccgataggtt tttgagcatc
    39121 gagcttatct cgcatttcta tattcttacg tatttcttca attgaaatcg aagcgactct
    39181 ttctccttcg ttcttggcag gaacttgctt ttcaagtctt tcatgatatc gtctttctgc
    39241 tcgtatatta ggagaagcat ccagatatag ttttacatca gcatctggaa agactactgt
    39301 tgtaatatcc ctgccctcgc tgacaacatc tttgccttcg gagatttttc taataataga
    39361 attaacttca gaacgaatct gtggtattgc agagacttga gagacaatag catctacctc
    39421 tggagttcta agctcttctt caagcctttc ttcatctaca aggaccgacc catcggctag
    39481 ataatcaaaa tggtgttttt tgacaagact tatcatttta tcgatatctc gccagtcaat
    39541 accttcgcga agggcaagaa aagagattgc gcgataaaga ctaccagaat ttatatataa
    39601 aaatccaagt ctctccgcaa tcatgcgtgc aattgtagat ttgccagaac cagcggggcc
    39661 atcaattgca actttcatta tcactttcct ttagatttct gattcaaatc gctaaaagct
    39721 ttgagtgctt gtacttcatt ttcggtgagt tcgcgccact gtcctggtgc taagttagga
    39781 tcgcaaatag ggccaaatcg aatgcgttcc agcagctttg catgaagatc gaaatactct
    39841 aacacccgac gaatctcgcg gttctttcct tctataagga taatgacggc gctattaggc
    39901 cctgtgacta ggacagagcg ggcacgatat cgtatcccat cgataataat tccccgcata
    39961 aagcttgatg cgaaattttc tggaatcggc acatcagtca agattctata ctctttttcg
    40021 atttgacctg agggatgcat tagttttgaa gctagatctc catcatttgt gaatagaaga
    40081 agaccactcg accattggtc cagtctacca atgttgtaaa ttcgctcatt cacattgccc
    40141 gaaattaaat cgatagcaca gggacgcccg tatggatcgc tgagtgaaga aagatagcct
    40201 tttggcttat ttaaaagaat atatcgaagt cttttgcgct gagttacaat gataggctct
    40261 ccatctattt ctattctttc attgcagacg attttttgcc caattgatgc ctttacgcca
    40321 tcaacagtta ctcttccctc tctaatcatt tcctcgcaag aacgtcgaga agctatccca
    40381 agagaagcaa ggtaagcatg taaccggatc gatttctctt taggctcact catccttcag
    40441 gcgtctccag tcttgagaat cttgtatttt ctacctcgtc tagcttcgga agatcttcga
    40501 tcgaattcaa tccaaaacat cttaggaatt cctgggtagt cccgtattgc atcggtttcc
    40561 caggcacatc acgcttgcct atctctgcta taagattctt ttcaagaagt agtctaatca
    40621 tcgcatcgac attaacacca cgaatagctt ctatctcagc acgagtgatt ggttgagagt
    40681 aggcaataat ggcaagggtt tccatggcag cgcgcgagag tctcatctcg ttttttttgc
    40741 catagtgatc ttttaactgt tcccaaaaaa ctagcttagg agcaaaaatc catcctgtct
    40801 ctttcttaat tggagcaaaa ccatgaactg gttgatcgaa ttctgaacaa attctctgaa
    40861 gagcaagcga agtctgctcc agtgtaaagc cagcaattct tgcaagtttt tcttcggaaa
    40921 gaggttcatt ttcaagaaat agaatcgcgc taattaaggc agcctcaaga tcaagttgtt
    40981 cgttcatttg aaggctccac cgccgaatat ggaattatca gaatatcgcc aaagagctta
    41041 tgctgacgaa cacttatcaa tttgttcttt gttgaatcca acacggcaag aaacgcacat
    41101 gcgagatcca tggcagaagt attccgagaa attaaatcat caaaatgaaa attgcccttc
    41161 ttgtccaata gctcatatat aagcgcaatt ttttcattga tactaacctc ttcatagaga
    41221 tcgattatgc gctctgaact aaagtttcta atcatcgaag caaagatcct tagcaaatcc
    41281 catgaatcgg ctggtatcca aggatcgata tctggattgt ctgcaaaagg caaggtgcgc
    41341 tgtgcgacct ttctctcgac aaaccactca acctccattt cacgctgttc cataagctca
    41401 gagagtttct tgaagcgatg atattctatc aactgatcga tgaggtttct tcgcggatca
    41461 tctagttctt cggcttcatc ttcggtgtga ggaataaggc tgcgcgattt gatataaaga
    41521 agcgtagctg ccatttgata gaattctgat atctcgtcaa gatccgcgga gggatcgcta
    41581 tctaaacatt gtaagaattg ttcagtgatg ctcgaaatac gaatgtcatt gatattcatc
    41641 tcattcttta ttattagata taacaaaaga tcgagaggac cctcgaaatc gcttagacaa
    41701 aaggttctgc cattagatat gaaagcattc ttttcttgaa tcgaatcatc gccgaattct
    41761 ttagtcattc tgaataaggc caatcataac gattgttgtt cacggggtaa ataccttgag
    41821 gtggaaggca agctgctaat tccctcagag aaatgccgag cccaggatga atcagttcgt
    41881 aatcaagctc tagaagaggc aatagagcaa attttctttc tgctaggcca ggatgaggaa
    41941 ttatcaaatc tggttctgca aatatttttt gatcatatat gagaatatct atatcaagag
    42001 tacgagggcc ttttttgatc tccttgcttc tatccctacc atgatttgct tcaatgcgat
    42061 gtatctgaat aagtaattcg cgcggtgtaa gattggtaaa acctgatact gcaaggttta
    42121 gaaaatcttg ctgatcgtta taataacgag cgcgagaacg ccaaagtcca gatcgcctta
    42181 actcataaag aaatcctcct agctcttgga tggctgaatc caaaattgaa gaggagtctc
    42241 ctttgtttga gcctagtcct aaccagactt tttccatgat aacaagcgct taaacccctt
    42301 ttgaaaatat gacatcagaa caaatcctct gttctgatct ttgagctatt attttcggaa
    42361 ttttctatat tggcagctgt attaaaagaa tcatttgttc cagaagggtt tgcttgtgag
    42421 ataattgctt cggttgtctt agacttatct ggactgaata atatctcgcg cacttgtgtt
    42481 tcgatttttt ttgccgcctc ggcattttgc tctagatatg cctttgcatt ttccctgccc
    42541 tgccctatct tctcgccaga ccatgtatac caagcgcctt ttttttcgat cacattgcac
    42601 tttacccctg catctagaag agaagcgctc catgagatgc ctttaccaaa tattatctct
    42661 agttcggctt tacgaaatgg cggcgctacc ttattcttta ccacctttac acgtaccctg
    42721 ttgccaattg cttcttcgtc ctgtcctcct tcgatagtat ctattcttcg aacttcgatg
    42781 cgaactgaag aatagaactt gagggcattg ccacctgtgg tggtttcggg attgccgaac
    42841 attaccccta ttttcatgcg tatctgattg ataaagacaa gaatcgtctt gctttttgag
    42901 agagtccccg ctagcttgcg taaagcttga ctcattagtc gagcctgaag tccaacatga
    42961 gaatctccca tttcaccttc aatttctgct tgtggtgtaa gggcggccac agaatctatt
    43021 acaattatat ccacggcacc tgaacgaata agggattctg caatgtctag cgcttgttca
    43081 ccattgtcag gttgagaaat atagagttcg tttatgtcta cacccagatt tcgagcatat
    43141 atcggatcca aggcatgctc agcatcaatg aatgccgcta ttccgccttt cttttgagct
    43201 tcggcaattg catgcaatgc cagcgttgtc ttgccagaag actccggacc atagatttca
    43261 atgattcgtc cgcgaggata gccgcctata ccaagggctt catctagaag gatcgaccct
    43321 gaagagatga cgtctattcc ttgagcaatc gtgtgggctc ctagtttcat caaagagcct
    43381 tggccaaatt gcttttcgat ctgtaatcgg gctgcttcta atgctctcag tttttcttct
    43441 ctttccttct gtatttctgg atttgggtca tcttttcccc tcattgcacc tacattttgt
    43501 ccaaacttag gttcctgttc tgtcatcaat tctgccgctg agcggccttg tctctttgcc
    43561 attgcaaata ccccttcatc ctctatgacg cacaaattgt atcacatgat tgaaatctct
    43621 gcagcctatc ttcaatatga tcactcatgg cggattaatt cagcaggtgc aatggaggat
    43681 gatctaaatg ctggaataag ggaaacaatc gcactgacta gtactccaac gcataagatg
    43741 agaattatat agctccaatt catggatata gggattcttt caagataata atcagggtta
    43801 agtaaggtta ttacataaga ggaagggtta agcaatcttg taaaaaaatt gatcaaagtt
    43861 tgaattgcta atattatatg attgatacaa agagatattg caataccaac agcgcttccc
    43921 aatatgcatc ctataatgcc tgtagctgtg ccacccaaaa gaaaaatgag catagtctgc
    43981 tttcgagata taccaaaaga gcgcaatacg gctatctctg ttctatgctc tattacaaaa
    44041 gtagtcagca ctgaggataa attgaccgct gctacaatgc ttgcaatagc cattatgaat
    44101 atgagcaaag atctggtgct tgaaaaggat ttgaacaaac cttgctctat atctcgccat
    44161 ggccgcacaa gagcttctgg atataggttc ttaatattgt tcatgatttt atcgagatca
    44221 ggtccataag gatcttttgt tttgattcca atatatgcat atgccatatc agaattcaaa
    44281 aggcgctgag caacttggaa atggattatg aaccaattag catcaagttc gcgatagcca
    44341 ctcgacacta ttccagctac tcgaaaaata ctcagtttcg ggagtatttc tgccccttct
    44401 gtttcgcgta aggtcatcag cgacacagtt tctccgagtc caacaccgag ggcctttgca
    44461 aggtaacggc ctagaactac ttcgttctcc ttttgaggga atggctctcc gacatctacc
    44521 tcaataaact gtctgtagcc tttatctaat gtaattgact cttccaagcc tctaatttgc
    44581 gcggaagtcg agctctttgc cgaggcagca aggcctaaac catctatttc taatgatgca
    44641 aagacaacac caggaagtgc gctaattgtt tctattctct gcgatcgatt ttcttcactc
    44701 agatcgaagg gaatactcat ctggaggtga ctagttttgg tttcaagata gcgttcagtg
    44761 atgcctttta tcattccatt tgacacaaaa atgacgatgt agagaggtac aatgctaata
    44821 ccaataccca atattgcacc aagaaagatc ccgtggcgag ccttatgcac acctttttct
    44881 ctagcttctg ctggtcttcc caaaagcata cgaaatccaa taaaagaaaa tggcttaagt
    44941 ctgctcagct ttatttgatg cattcgaatc gacctccctt gaggaggtag gaagaaccgc
    45001 caatggcggc caaatcggga tcatgggtga ctatcaaaag agttgctccc gtcgcttctg
    45061 gaagattctg aagcaaggat cgcaattcct ttgcgcttga agcatcgaga ttcccagtag
    45121 gttcgtctgc aagaacaatc gacggggaat tgattaatgc tcgtgcaacg gccaccctct
    45181 gcctttctcc gccagacagt tgtgaaggat agtgatctag acgcttgccg agtccaacca
    45241 tatcaagaag ttttcgtgcc ttcttccatg cttcatgtcg ctgtatacct gctacataac
    45301 tcggcagggc tacgttttcg agcgctgtaa aatcattgag taagaagtga aactggaata
    45361 caaatccgac aacacttcgc ctaaacatag agagggattt ctccgaaagc gcggatagat
    45421 catagccgtc gacatacaca tcacccgaat cgaagcgttc aaggctgccc aagatagcta
    45481 ggaatgtgct cttgccggag ccactagggc caagaattgt gcatatttcg ccctttccca
    45541 tttcaaaatt cgcctgatcg agaatgcaca atacttcggc gctcgaagca aaggtctttg
    45601 ttaagcctct acactctatg attggcttat tcacttctca gaacctccaa cggagatgca
    45661 tttctaaagc gacgataggc tagatagaca gcgatgaccg gaaagataat cgcaaaacaa
    45721 gcgataagag ctatatcgga aaaaaatatt cgagacggaa taccctctat atagaatatc
    45781 gaaggagaga agaccctgag atcaggcact cctttgacaa gtccaatatg atacagagta
    45841 gatatgaatg cttcgattaa cccaattgcg ccatttatta tcttatctac atgctgagca
    45901 attggaatgc caatagcaat accaaatata gcgcctccca atcctaccaa aataccctct
    45961 agcacaaaaa tggtcgctat tgaatgctga ttcattccaa tggctgccat tattgctaaa
    46021 tctcttgatt ttctagctat gctgcgacgc atcgagtaat gaatgttgat agctacgact
    46081 ataaaaataa tgccaactaa taagaacatt aaaatctttt cagtatgcaa ggcgctaaaa
    46141 aagctgcgat tgtattcctg ccatgattcg atatctaata ttccattttc atgttcaagc
    46201 attgaagcaa aagaattagc gttttctgca tttatcagct ttattccaag aatagtagaa
    46261 gctggaaagt tttctaagtt cttagctttt tgtctgggca taatagcaag cgcagcatca
    46321 agctcgtagt atcctgagcg gaagagatta gagactttaa ctctttctga atattgttga
    46381 attccctcct ctgcgtctac ataggctcct cttaaagtta gcatagatcc ttcagataca
    46441 cctaaaatgt gtgcggcttc ggaacccagt attattccct cttctagact cttggctgca
    46501 gctgaaggag aaatatttaa ggctctacaa taaccgaaat ctagaactaa atcctcggaa
    46561 tctatccaca ttacccgaag cacattttgg cgtccttcct ggccttgtac gagtagattt
    46621 gtctccatga agggaatcac cgatcttact aagcgatttt gtcgcaagtt attggtaaat
    46681 atatcgaggt cgccttcttt aactcttgct cttatatgaa aactcgttgt ctcaagaagt
    46741 gattctatgt attgctgctg aagaccattc ataacgctaa gcacgactat taaggcaata
    46801 actcctaccg caattccaag agaagcaaac tttagtccag aactatgggc ggattttttc
    46861 cctgttatcg accaacggac tgcaatgcgc agcagcggct caaaacgcat tccttacagc
    46921 ctccagagct gtttttcttc atccgaagtc ttgatcatcg accctatata cctcagtctc
    46981 caacaaaatc gtccaaatat ctatcgatat caaatggctc aaggtcacca tacttttcgc
    47041 ctgttccaag gaatactgtt ggcagtccaa attgtttgct tacggctatg atcatgcctc
    47101 cctttgaagc tgaatcgtat tttgccataa ttacgccgtc tattttgacg gagctattga
    47161 atatctctgc ctgtcgcata gcgttttgac cagttgtagc atctagcact aaaagccttt
    47221 ggtagctcac atctcctgcc ttctggctca ctacacgatc aagtttttca agttcacgaa
    47281 gaagatcggc cctggtatgc atccgtccgg ctgtatcggc aattacgagg cgcgcattat
    47341 ttttcctagc agcatctatt gcatcccata aaaccgctcc agggtctgat ccatgctgtt
    47401 gagctattac acgagctcct aacctttgcc catgaatctt aagctgctca atagcagctg
    47461 cacgaaaagt atcgcaggca gcaaatatga taggctgttc tattccattg ttttctgccc
    47521 atcttgctaa cttagcacag cttgttgttt taccgacacc attaacacca agtattagaa
    47581 caatattgag acgtttatca tcaaagtcga aatctgcgat tttaataaaa ggacgcagta
    47641 tttctttcaa tttacccctg gcctcctctg gggtggaaat gccttctttt ctgcacacat
    47701 ttttaagagt atctacaacc tcaaaagcga ttgaggcacc gatgtcaccc tcaatcaata
    47761 aatcctccag ttcttcgaat gtttcctcgc tcaattgact tctgtgaaat aatgctttga
    47821 ttttgtcctt aaaattcatt ctttctccta gctgtacata ttattcagtg agatgctcca
    47881 agcataatac ctgcattcca tatcgatact gtcaaaaatg cagcggtgac cgcagcacta
    47941 gtcccagcag ctatagctga gatagtcatt gcattattaa acgcgctatt tctataagca
    48001 gcctcacgat atatttgcca aaagccaaca gcaacaaaag ataccgaaag actaattaca
    48061 actcctccta ttgaagtctc aaaggcggtt tgggctttct tatatctatc tctattttcc
    48121 catggatcaa cagaaggcaa atcgatttta tcgatcgcta attccgaata agcgagtgaa
    48181 ctcttcttgg caataagtgc gctttctttt ataaaagagt ccacagtttc tctagcagca
    48241 tcgagtaagc taaagactga aaagctgttt gataaaaaag cgtaactttc ccctgttttc
    48301 tttagaagaa aagatcctaa ttgcatattg ctgtcttcaa tcgtatacat cataaagaga
    48361 accgcatctg ctttctggag agagctttcc agaaatagag cggatttatc ctgaataatc
    48421 aaactccaat tccagttctt tagagtttca atatcaattg ctgtcatttt ttcaacagtt
    48481 gtgcgcgatc gcaaggtttc ctccatcact cttgataagc tccttgattc cggggagaca
    48541 gtattgttcg tatcatactg agacattgaa agcttatcgt gcacaaaaga aagaaccatc
    48601 atcgaatcta cgctttcttt ccctaatgga tccagatctt gtatatttga gatttgtaaa
    48661 ggaaaaacaa gcgcaataga acctatgctt tgcgaagggc tagcctgggc aaatatataa
    48721 gtatcaatga ggagaaagag aaatatcagt aataaatttt tatatgtctt catcttcctc
    48781 ttcaacggcg gtgccgcctt tccattgcca atgaaggtaa gcttctaaga atggatcaag
    48841 gtcgccatcc attacatttt gaacatttcc aaccgaaaac ttgttccgat gatcctttac
    48901 cattgtatag ggctgaaaaa cataagacct gatctgagac ccccaagcga tctccttttt
    48961 ttcgccagcg aattttgcag tttctctgtc tcgctctgct ttgtaatact cgtacagcct
    49021 tgacttaagg atgctcatgg ctacatcctt gtttttatac tgactacgct cattctgaca
    49081 tgtaactaca atacctgtag ctaaatgtgt aattctcact gcggagtctg tcttattgac
    49141 tttttgccct cctgctccgc ctgctcgata tgtatctatc cgtatatctt ctggtcttat
    49201 atcgatttcg atcgattcat cgagtacagg cattaccgat acagaggcaa aagatgtgtg
    49261 ccgtcttgcg tttgcgtcaa aaggggaaat ccttaccaga cgatgaactc ccgtctctcc
    49321 ctttagataa ccatatacat attcacctga aatctggatt gtaactgatt ttatgccgcc
    49381 ctctgcttcg aggaagtcca gagtctcgac cttaaaatca tgcctttcag cccatcgtac
    49441 atacattcga taaagcatac tggcccaatc acatgcttct gtaccaccag aaccagcatg
    49501 aatagtgagg taggcgtcat ttcgatctgc ttctccggag agaagttcaa taaccacagc
    49561 tctgtcgaaa cgtgtcttaa tatcttcaag actagcttga agatcagcct ccatgtcctg
    49621 ttgatcctcc tgatgtgcta attcgtacat ctcttcaagt gtaagaatat cattatgcag
    49681 ctcttgccaa gtttccaagc gatctctcaa tgtctttatc tcgctgaggg tttttttagc
    49741 cttggtctgt tctgaccaaa aatctggaat attagtttga gcctctagat catagatttt
    49801 ctgttctata gaaggcgcgt caaagacgcc cccagatttc aaaaatgtct tcttttagat
    49861 cttctattgg ttttgcaata tcttctaaca tgtgcaactc ctacatcaat attgtatatc
    49921 aaatctatat actgtctcat gttcccaggg cttccctgga aacagaaaac aagaaggaaa
    49981 attctgttga tttggtgaat caggatacat ctctgtttct agacaaaagc cagcatattt
    50041 atcgtagata gagccacttt ttccagttat tgcggctagg ttgttacctg tataaaactg
    50101 aacggctggc aatgtggtag ctacactaag atttcttcca gtagatggct cagatacgaa
    50161 agcgaaaggc tttaatggca ggtaatcctt gtcaataaca tagcaaaaat cataaccatt
    50221 gcctacatct gctatatctc ttccaattcg tttagtgaat ctaaaatcga gagctgtccc
    50281 atcaacaggg caaatctctc cttttggaat tagatattca tcaacaggta ggtacatgct
    50341 tgcctgcatt tcaagttcat ggtcaagaat agtccctttc ccctcgccag caaggttgaa
    50401 ataagcatga ttggttaaac ttataggggt cacttcatct gtctcagcat aataatgtag
    50461 acgcaattct cctgaagacg atagagaagc aataacctca acatagagtg tgcctgggta
    50521 tccctcttct ctatcagggc tcatcctgga aaatctaata gctggttccc ctgaaaaagc
    50581 tgataattca tgattccata gatgcttatc aaaccctttg cgaccgccat gcaggtggtt
    50641 gcaattatcg tttttccaga gtttgtatac tatcccatta agtgtaaatt gagcaccgct
    50701 tattctgttt gcaaagcgcc caatagttga accgaaatat ggatgctttc ctacatatcc
    50761 agagagcatc gaaaaaccaa gcaatatgtc gtccttttgg ccttctttgc cgggcattat
    50821 aaaacttacc catgttgccc cccaatcagt aaaacttgct tgaaaagatc cagccttgag
    50881 aataaatagc gaaacttcct ccccgctcga aagagtgcca aaatgttttt tttctatagc
    50941 tgatgttgca acatgcatat atttcccttc tctatgccta ctatagaggg ctttatcata
    51001 gcgcacaaga aaataattct cgagaccttt ctatgttaaa gacgatagta cattctataa
    51061 aagacttcaa accaggcatc aggaatgagt cttcgaatcc atactgcaaa ccgctcaaaa
    51121 aatggcccta ttgttaagcg agtgggaagg tgctttgaca cagctaatcc agcaatcttt
    51181 ttgcccgctt tcgctggatc caatccattt atctcgtcat gttcctgtac acctattact
    51241 ctctcaaatt gatcatagta cggactttcg ggtgtcataa tcttctgtct agaaccagtg
    51301 aacccggtct tgaaatctcc tggctccaca atgcaagctt cgatgttaaa tctgcgcacc
    51361 tcatacctca acgattctac aaaaccttcg agagcaaatt tacttgcaga atagtaagct
    51421 tggaatggca ttccgatctt tcctgctata gagccaacaa tgataattct ccccctacca
    51481 tgctgacgca tactaggtaa gaaagcacac actactcttg cagtgccaaa gaaattcaca
    51541 tcgaactgag ctttagctaa atcaatagaa gtatcctcga tcgatccgct aattccaaag
    51601 cctgcagcgc atactagaag atcgatgtgg ctttcctgag aaagcacaaa atctctgctc
    51661 ttgtttactg attcatcaag agccacgtcg gttggtacat agataaaatc ttgaggcaga
    51721 tcaggaggtt gctggcgacg tccggctcca tagacaatgt atccaatgtc aagcagcgat
    51781 cttgagatcg caagaccgag cccactcgtt gcgccagtta caaaggcaac tcgcttatta
    51841 tcaagaactg atgccatgca gattacctca atgaataaga tggtatgcat atacgatgat
    51901 atcggcaacc tgggctgcag accatcgctc agtgtctatt acaagatgag cactagagat
    51961 atcatcgata tcgatattgt aaagtgcaag atatcggccc ctatccttcg catctctctc
    52021 tttggtaaaa cggtctattg cttcaatatc accgccttct cttgcatgga ttcttttaac
    52081 acgtgtggca tggctagcct tgagatagac ttttagctca gcttcaggca acatccagat
    52141 tgccaatctg gaacctatca cggtattacc ctttcgtgct agctgaacct gatgttcgtc
    52201 taactttcga tcatattcga ggctttcctg cgctttctta tgtagctcct caaagcttat
    52261 ccccatgtct gctgccatac tgcgaaaagt gtaattcact ggttcataac caagtttttc
    52321 agcaaccaaa tgcagcacgg ttgaattgcc acagcctgat tttcccgata ttgcaatgat
    52381 acccatatct atattactcc acatttctaa gctgttctct aatgttctcg agagcgtctt
    52441 tcatctcgac caccgcatga gataccggga caagcatgct ctttgagcct atcgtgttga
    52501 tttccctatt catctcctgg catagaaaat ctagtttctt gccacaaaca ggttgcctag
    52561 caattgattc gaaggcatcg atatgggctc taagccggcc tatttcttca ttgatggtaa
    52621 ggcgaacaag ttgcaccgct acttcctgaa gcactcggct ttcatcatag ttttcggata
    52681 ataattcttt gaatctagtt actatctgtt catgcactat tctttcgagc gaatcggctt
    52741 gaagttctat ttcattaagc ttcgatttca accttttaag ctgtcctctt atattctctt
    52801 ctgtggctgt accttctcga attcgacttt cctcaaattc ttgccaggtt tcttcaagta
    52861 ttggcatcaa tataggccaa aagttgttct catcaatctc tatattcata tcgacaatgc
    52921 caggaaattt tgttatgagg tcgagcgaca agtctttttc tatattgcat gctttagcaa
    52981 gcttgcgcaa ttgttccgaa agtagcgtgg ctgcttgaaa ttgaagctca atatcttcaa
    53041 gcgagagctc tatttttttt aacttaagag tgcattcgat cttccctcga agtatgcgag
    53101 atcctaaata atctcgaatc aaaggctcaa ttcgatatgc atatggtgga agaaaaatcg
    53161 atatgtccaa aaaacggtta ttgtaggatt tgagtgtaag ggtgccctga aaatcctttg
    53221 ccaatattgg actatgggca aatccagtca tactcttcat tgtttttgtt ctcctaacat
    53281 ctcaattact tcgatgttgc aggtttttta tccaatctct cagcaagagc tttagctagt
    53341 ttccaattat cgccagcacc caatgtcaga aaaatatcac ctggttgtaa tagattagta
    53401 agaggattca gtgcttccaa gggctcgtca gcatatgcaa taaagccttt cttatcttct
    53461 ctggtcttat tcttatagca ggtaatcatc ttttctctgt tatctttttc taagtctatt
    53521 ggaaaaagtt ctggacgatg ctcgcaaagc ttctcataga gagttttgcc actgaatccc
    53581 gagacaggct gttcacgcgc tgaagcatat atcttgtgca acaagataga gtctgcatta
    53641 tccagagaag aaacgaagtc atcttgcagt gcaattgtcc ttgtataagt atgagacata
    53701 aaatccacca caagccttct atggggccaa aatttcttta ttccttcgat tgtagtctta
    53761 attgctgttg ggtggtgccc ataatcatcc atgataagga tcttttcgta ctcccctatt
    53821 atttcgctac gtcgctttga acccctaaaa gtcgccaatg cttcgactat ggaattccac
    53881 tcagattcct caaacgtttt cgatttttct gtttcatata tctgctcgac aagagcgatt
    53941 gctgcaaccg catcctgaac cagatgtttc cctggtaaat gaatccttat tggaaaagaa
    54001 ttctctccta ggataaaggt attgactccc gctttggtct gaaaatcgcg aattctgtat
    54061 tttccatgag ctaaaaaacc atactctaca tatttgatat ctttcctttt ttgtaggaca
    54121 aggtctgcaa cctctacagc gcccttctga tcagcacaaa agattagaac accattgaga
    54181 ggaagactaa ggacatactc cacaaatgca tcgcgtatgc tttcgtatgt cggaaaataa
    54241 tcctgatgat cgctttccac tgatgtaagc actatccaag taggcttgaa gttaagaaaa
    54301 tgtttgcggt actcacaagt ttcggcgata aaataagtct ctccctggac taaagtacat
    54361 ctatcgtcaa aattagaaat cgcagaacct gctagcacca ttgcaggcaa tcctaatgct
    54421 tttatgagta tacccgacaa agcagctgtc gtagttttgc cgttaacacc ggctatgcca
    54481 gcagacctgc tatgcaaaga aagagctcca agcacttcag gatagctaaa tattggtata
    54541 cctcgatagg tagccgcctt gagctcagga tttccttcac gcgaatatgc tgcagagtga
    54601 ataacaatat cggtgctaag aggtagatta ttcttgtcga agttttcaaa aagttcgaca
    54661 ccaagcgagt gtagaatact atcagtatag aacacatcag ccacatcgct acctgtaagg
    54721 attgcgcccc gcttttttag tatctctgca agtgcagtca ttccagtgcc ttttgctccc
    54781 acaaggtgaa catgaaggcc ttccagcttc tcaggaaatt gagtcatctc cattattctc
    54841 ctattctaaa gaaattcatc ttctaacctc aagaccccgt ttatttgaag atatatatat
    54901 cgactaatta ctcaatgctc caaatggaca gatagagaaa aaaagaccgt tccttcagga
    54961 gggatagaca agtcccatcc aatattgatc gaaaagcctt cgaacatgag aaaatctgat
    55021 ggaacattca agacttgcgg agagactgta ggcttttgca agaaattaga ttttgcgaat
    55081 agtgaaaacg gcgaatcaga acggattaac actctctctg cttttctaag attgctaagc
    55141 ccgataccat cgaccatttc gaaggataat tgcgatttca aatctaaggt tttgctctct
    55201 cgatgatgta ataattcaat cctatgatcc tcaaaaactg gagtggcaat aatctcggat
    55261 ttagtacaaa agcgaaactg gcaagcttcc gtattcttat tgctcaattc ataatctatg
    55321 ctgaaaaagt catttctaaa gcgataggtc tttctacaaa caagggtcaa aaaatggcca
    55381 taagacctat tcgaaaattc tctgcgaaaa agaacactcg actcctcttt tggattctct
    55441 accatgttcc aatgctctat tgctatgata ttactttcga agcttccttc ggtgcttata
    55501 taatctttga agcatcctgt ggagcattga tcgtcattcc atccgcaggc atagtttgtc
    55561 tctgtcttta tagagtcaaa ttcgcttaca gaggcgaatt ccgattgtaa atagcagtta
    55621 taaacagaag attgaaatat tgcttccttt aagccatcga aattcagatc gtcaaatgat
    55681 agatacgagt gaaatctgct ctgtcgtatt gttttttcag cctctataag cgaggaaaaa
    55741 gcacctagcc ttgcttctgg aactaggatt ccaccagtgg ggccgatcca aaaaagatcc
    55801 ccgcattgag ctttccagac atcatcgata gaggctttct ttctcgattt atcaccccta
    55861 aaaagcgcta tcatagtaga gacatagttc aatttttgat aaagattatg tacaagtaga
    55921 tgatctaata cagattgctt agaaatttga atatatcctt gatttttagt gggcaagcga
    55981 ttgattgtct tcttgcaata atccttaaac cgctgtgaat agcattgaga aaaatatatt
    56041 atcctagtgg gttttgtggt tttatggagt tgaataggag taagggtgtc atattctagg
    56101 cagtttttct gaaaccatgc aaaagtgcgc tcgaaaagga catccggaga ttcgaggccg
    56161 ctcttctccc aactggtgct tatttcgttg ccatctgcca taatgatgaa tgtcttgcat
    56221 tcgggatatg attcttgaag atccaggaga gtattttcat aaggtacaaa gctattttct
    56281 gaatcccttg attcgaaagt gggaaatatc gttatcactt ttcgatgatc ttccgaagcg
    56341 attggcatgc agaattctct gcttccttgt tcgtatgaaa agtattctgc gggcaaaaaa
    56401 gtatactgga tttttgagtt ctgtaggatg gatggtatag aagagctcca agaatattca
    56461 tagagccatg caccagaagg tcttttgcca gtggttttac gaataaaagc acttaagctt
    56521 tcgacctgtc cagtcatatc ctgcgtgctt ataagaggcg aaagcggatt gtataaccca
    56581 cctcctaaaa gctcgacctg tcctcttcta accatctcta cgagaaggta catatattcg
    56641 ggatgatttt cttcaagcca tggaaaaata gcaccagaaa agtagattac cgatttaatg
    56701 gagagaaatt tgtatagccc agagagaaaa gggcgccacg cgccttggta tgtctgttca
    56761 tataatgctc ctgatgcatt ataggggaga tgattataca tccctattac cactttgaag
    56821 ctctgcatcg aagaaatata ctcctctggc attgaagaac tacttagcct tattacatag
    56881 agtgtgcgag atgctataga ctctgtcaag catgatcgaa gcacgtacag ccgttgacct
    56941 acggcttaca aagcattatg gtcaattcgt gggcaaattg ttgaccatca ctcttagtag
    57001 agatgacgat ggacggcgtg ctgatagaat agtccgaaag gtgctcaaag acactgctct
    57061 ttcaacaatt tatcgtcttt ttcgccaggg agatatcagg acttcaaata gccccataaa
    57121 gccgaatgat agattaaaag aaggggatat tgttgagata aggctcccag aaaaaatttg
    57181 ggagaaaggt catagccaaa caataataaa aagtgcttcg cttaaaaaaa agcattcaca
    57241 agggtcttat gatgaaattc caagtatctt gtggcagaat agccatctta tcgctttcca
    57301 taagccgaga ggaatgttag tacatgatgg aaatacaaat ctagatgctt atgtgcatag
    57361 ctttcttcac gatctattgg agccttctgt atcattttct ccaggaccgc ttcatcgact
    57421 agataggaac acctctggaa tcatagtatt ctcaaaaacc agaatcggcg cagacctttt
    57481 cagcactgca atgaggaata gaaggattca taagttctat attgccatag tcgaaggaag
    57541 gatagatgag ccaatcttac tcaaggattc tattctgcgc gataagaaga gacgattgag
    57601 tgtcatagat aaaaatgaag ggagggaagc tatccttcgt gcattcccat tgatacagag
    57661 caagttattc agccttttgc ttgtagatct cataaccggg cttacacatc aaataagagt
    57721 gcaactagca tctcacggct acccacttgc aggagattca aaatatggag gatctaagac
    57781 acctgaaatc aacagttact ttttgcattc atgctgttta ggctttgtac aacccctttt
    57841 tgaagacatg cctttgacta tttttgatcc tcttcctaat gattttgggc ttgctatatc
    57901 ctatttattc aatatgaata agagagaaat tgatactctt atatcccaca ggatagatca
    57961 gtcattggca atatgaaaca ataattcatt tttcaataag gaggtaagac atggcagata
    58021 cgcctaatcc taagaaaatg tggaaaataa aattaaaatg gcctctcgtg ataactataa
    58081 taattgcact atgtgtaatc tttttcctgc tgacgagctt tgtcattgta gaccaaactg
    58141 aaaaggcggt tattacaacc ttcggaaagt atacaaagac ccttggcgct ggacttcatt
    58201 acaaattgcc atttggaatt caaaaggccc atcttgtaaa gactcaggtg atccaaaccg
    58261 agacttttgg ttttagaact gtaaggccgg gaatagtcac acaatattca agcgaaaaat
    58321 tccccaatga atctacaatg ttgactggag atctcaatat tgtagatgtc gaatggatca
    58381 ttcaatatcg tgtcacagat ccagaagcat ggctgttcaa tgtgatggat aaagaaaaaa
    58441 caatcagaga tacttctcag acagtaatca atatgcttgt gggagataga actattctag
    58501 gtgtgatagg ccctgaacgt cagaatatcc aggaagctgc cgtcgccctt atgaacgaat
    58561 tcttctcgaa atatggtctt ggaatcacgg tgacacaggt tcagcttcaa aatattgtac
    58621 ctccggaggg cgttcaagct gcctttgaag acgtaaacaa agcgatccag gatatgaaca
    58681 gacttattaa tgaagggaga gaagcatata acgcagaaat tccaaaggta caaggtcaag
    58741 cagaccagat actcgaagtt gcacagggtt atgcttcaga gcgtgtcaat gtagcaaagg
    58801 gcgatgtctc tcgtttcaac tctgtctatg acgaatatcg caaagcgccc gaaataacgc
    58861 gaaaacgtct atattacgaa atgatggagg aaatattcaa agaccaagag aatatagatc
    58921 tcatcgacaa atcacttcag aatgtattgc ctataaagaa tttagctcag actccttctt
    58981 ctcaggaggc ttcaagatga aaaaaacatc aataatcata acaattgttg tggcattagg
    59041 aattcttttc atcataatct ttgggcctct ctttgtgcta aacgaaggtg aacaagccgt
    59101 ggtggtgcgc tttgggaaaa tagttgcttt tcataccagt gccggattga aatggcgaag
    59161 cccttttgtc gatactgtca tgaaatatcc taagaagctg atgtcctggg atggagaacc
    59221 tcagagaatt cccacaaagg aaaatcaatt tatctatgtc gatacaactg cccgctggag
    59281 aatagtggac ccgataaaat tctatgaatc tgtaaatact ctcgatggag catttagcaa
    59341 gctcagcgat gtaatcgatt cggcagtgag gactgtcata tcctcgaatt atctgcgaga
    59401 ggctgttcgc agcagcgatg aaattttgtc agaaagccaa gtagaaagct ttcaaacagg
    59461 ggatatagaa aacaacacca cgcttcagca actagccata acacaaactc aatacgaaaa
    59521 aatcgagaaa ggccgtttgg ttcttgcgga agaaatgaaa accctcgtct cggaaatagt
    59581 cccatctttt ggaattgaag tagtcgatat ccttccgcga caaatcaagt attccgatga
    59641 gcttagtgag agtgtatatc agcgaatgat caaggagcgt aatcaaatag cgcaagcatt
    59701 tagagcttat ggcgaaggga aaaaagctga atggctcggg aaactggaaa atgagaagcg
    59761 cacgatactg tcttctgcat atgcaaaagg tgaatcaatc aagggtaaag cggatgccga
    59821 agcatcgaag atctatgccg attcctttgg gcgagatcct tcattcttcg aattctggaa
    59881 agcaatcgag tcatatcgaa agacaatgcc tgcattcaat aagaccttat ctactggaat
    59941 ggactacttt aaatatctat atagtcctac tggtcgctga tagattgctg aaatatcata
    60001 tcgaccttag ttcagcagca gccttccagc ctttaggata tcttttaagt agaattagcg
    60061 cgtacgaacc atgtgcagct ggtccaggat taactaccaa ggtcctattt tcgatatgaa
    60121 tgcttttgcc ctcatgaata tggccgcaaa tccataaaag tggctcatgt tcatagagga
    60181 atctctgaaa ggctatgctt cctatatgtc gcgaatgacg tatgtccgca aaggtatctt
    60241 tgggcggggt gtgagtcaaa acaataagat cgggggtgag ctctgaactg tcaattatat
    60301 ttctataacc ttgaatggca catttgtaag catggtttaa accgttttcc agttcttcct
    60361 ctcttatttc gaatggcgtc agcccagtat gaattagacc tccaccgcta ccaatgatcc
    60421 atatatcctt ttcagaaaga agggggaagc atcgctgttc aatatatcca ggttgctttt
    60481 caaagtacct tcttgcttct atagtgtcgc aatttcccgc caagaaatat agcgcagctt
    60541 tggatgtaat gcttgaaaac gccaatagca cattctgagc ttctttttct tttccgaaat
    60601 tcgtaagatc cccagcaaca agaattcctt ctatgttagg atttgccttt aatatctgtt
    60661 ttagcgcatc gatgttgcca tgaatatcgg caagggcaag gaataaatgc tccatactat
    60721 tcttatgact ttggcactac tcgcgcaaca cgcttctttc cagctctgag cattagtgca
    60781 ttgttttcta aatcgcgcga tgtcaagatt tgcatttcgt tttcgatttt tctgccatta
    60841 accgaggcgc cactttgttt aaccaatctg cgcgcttcac tcttagaggg cgcaagtcct
    60901 gcttcaacaa atagatcaag cactccaatg cctgaatcta agcgggatat ttcaacctcg
    60961 attgttggaa gttgggaagc gtctccttct cctctaaatg ctgccttggc ggcactcagt
    61021 gctttgttgg cctcttcttc gccatggata agggatgtga cttcccatgc aagtcgctcc
    61081 ttggcttcat taagcgcttt atccttgagc gaaccgagat atctgcattc gtctatgggc
    61141 agaaaagtaa acatcagcaa gaatctctct acatcgctat ctggcacatt cctaaaatac
    61201 tggaaaaatt cataggggct tgtgatttct ggatcaagaa agagtgcacc tttttcggtc
    61261 ttgcccattt ttttcccatc gctagtggtt acgagtggaa aggtaagccc ataacactca
    61321 gcaccctcta ctcttcttat tagctcaatt ccagctacta tgttgcccca ctgatcgtcg
    61381 cctcctatct gtaatctaca accatgaagt ttgtaaagct gaagataatc atagctctgc
    61441 aaaagttgat agttgaactc tatgaaggaa agccctgtct ccagtcgcat tctatagctt
    61501 tcaaaggaga gcatcctatt caccgaaaaa tggcgaccta tgtcgcgcaa aaattcgatg
    61561 taattgagat tggataacca atccttattg ttagctgtaa gtgcattgac gccatcgaaa
    61621 tggataaaac gatcgagctg atctataaat ctttcagtat tagcatctat ttcttcatag
    61681 gaaattagtt tcctcatctc ggttttgcct gagggatctc cgatgcgagc tgtcccaccc
    61741 cctagaagcg ctatgccacg atggcctgca tctcttagat ggcgcattgc aaaaaacgga
    61801 accatatgac ctatatggag agaaggacct gttggatcag ttcctatgta gaaggttatt
    61861 ggcccttcgt ccataagatt ggaaagccct tccagatcgc tgcattgctg cacaaaaccg
    61921 cgttctttca agatctcgag cgctctgttc atgaaaaatg ctcctttaag ttttgcagta
    61981 taggatgaaa ttggcgcttg ctcaagttat tctattgttg tatttctaaa aaagtcattt
    62041 cttcttccaa aaagtgagaa ataccacaag ttttcaatag ggtgtacgac aattttgtgc
    62101 aaaggtagct aagcagcatt tcttcgagcc caat
//
LOCUS       DAOSOB010000003        58008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_587_length_58008_cov_72.934637, whole genome shotgun sequence.
ACCESSION   DAOSOB010000003 DAOSOB010000000
VERSION     DAOSOB010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 58008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58008
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_587_length_58008_cov_72.934637"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1504)
                     /locus_tag="PLS88_00535"
     CDS             complement(167..1504)
                     /locus_tag="PLS88_00535"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOU05877.1"
                     /translation="MVILTLNCGSSSVKYQVYDWEKKDVLANGIVERVTIGGSFITHK
                     VRNRPDYEVEHECPNHVVAIELILKTLVDPEVGVISDLSMIKAVGHRMVHGGSKFAKS
                     SIINGEFLETFKSLNELAPLHNPANLMGVEAATTILPNVPQCAIMDTAWHQTMPASSY
                     MYALPREWYEKYMVRRYGFHGTSFLYNAKRAAVLLGKDPFETNLVIAHIGNGASINAV
                     KNGCSYDTSMGLTPLEGLVMGTRSGDVDPGIIFHIMRKTGMTAAEIEKKLNKESGVLG
                     ITAKWADRRDVANAAEKGDPAAILAQQVEAYRIKKYIGAYYAALGHVDAIVFTAGVGE
                     MSPVIRNLATQGLEELGIAIDEKKNALAMCRNAELDITGSSSKVKIFIIPTDEELVMT
                     EDTIALINGTYDVHTNYRYYFENRDYVNRARAEGLQRDLEKKPWLKDIVARIP"
     gene            complement(1643..2044)
                     /locus_tag="PLS88_00540"
     CDS             complement(1643..2044)
                     /locus_tag="PLS88_00540"
                     /inference="COORDINATES: protein motif:HMM:NF015166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05878.1"
                     /translation="MNIITLHGAFMGLGLVCMVVAIIIAHYYRSKKWWLKLHRGLNIA
                     APVLAVIGIIVAIIMVTPGYGVQLPLVHHLLGLLTLVLLIIEPILGFSIFKSKDKQRI
                     ASLKKTHRIIGRITPIMYIVTMIAIELVVGG"
     gene            complement(2086..2643)
                     /locus_tag="PLS88_00545"
     CDS             complement(2086..2643)
                     /locus_tag="PLS88_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON domain-containing protein"
                     /protein_id="HOU05879.1"
                     /translation="MKLRAYVIFCMFSLFVIVPSSFAITIDGAISSGEYSKQTEFDKG
                     NFKLLWEFEGEKVFMAIIAKTPGWISVGFNPSSFMSNSDLILAVVKDSQDIKVYDEWS
                     SGMFGPHAPDIEKGGTSDVLSYAGSRSGDTVIFEFSRLLDTKDKYDKAIPTSGKFKIM
                     WAYGPSLDMTAKHKKAGTATVEMAK"
     gene            complement(2753..4870)
                     /gene="priA"
                     /locus_tag="PLS88_00550"
     CDS             complement(2753..4870)
                     /gene="priA"
                     /locus_tag="PLS88_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOU05880.1"
                     /translation="MPMSFVRIAYPIPLEETFWYRNDTSTRAEIGMRVEAPLGRRAKV
                     VGYVVESIDIPVSENELNLGAESQDESSAKKPKLTQLTKLAKPELPAYLEKLELPTDL
                     IKLEPSKIRSINRVIDIEPLFDSEMLELGRWVASMYHCSLGEALSAMLPSARRERLEI
                     AEQFDEIEVSRTPLVLTEGQRAALEGILAKPRGMFYLYGPTGTGKTEVFLQLADATLS
                     MGLGVLYLVPEIALTRQVEQDARARFGEQCAIIHSRLTPSRKLAEWRRIAKKEARIVI
                     GVRSAIFAPISNLGLIIIDEEHDGSYKSGTTPRYHARQVAMRRANLAGARLVMGSATP
                     SPEAWQACEEGQMRRFNLSNRPAGGSFPEIHVIDMRGRHGLISEELAEALHAAKQAGR
                     QSILFLNRRGFAHTLMCQTCGEQLLCKHCSVPLTYHKASNRLICHYCGYHEIKPRACP
                     SCGSLDLAWASYGTERIEEELNEGFPEMSHARLDTDTTNTKGYLENTLLSFRNGNIDI
                     LVGTQMVAKGLNFPGVRVVGILMADQGLAMPDFRAGERVFSLIVQVAGRAGRHVPDGI
                     VFIQTKKPDSPIIQLAAEGDVPCFLERELQIRRQLEFPPARRLCRFVFRSKSAKGARN
                     EASKYADLARRIVCISPFEDIDILGPAECPLAVVADNHRYQLIFKAARFSDLQRFVKT
                     LRHSIEPTSSVYIEIDIDPVQMM"
     gene            complement(4909..5685)
                     /locus_tag="PLS88_00555"
     CDS             complement(4909..5685)
                     /locus_tag="PLS88_00555"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOU05881.1"
                     /translation="MSEKALNENESTYNESTYTDAEARQILETLFLIEDFFRYGVRCE
                     HKISLEAPSSAIPSTWDRKAHLEALETQVSQCTACGLWQSRSQTVFGEGVLDPKVMVI
                     GEGPGMEEDRQGLPFVGASGQLLDKMLAAIDLSRHNNCYIANIVKCRPPNNREPSPSE
                     CDACIGYLRAQIDILQPGFILCVGRIAAHSLLRLSDPISRLRGRSFDFQGIPVRVTFH
                     PSALLRDTALKRPAWEDLKQFRSLMESRALTESRATKKVK"
     gene            complement(5792..8422)
                     /locus_tag="PLS88_00560"
     CDS             complement(5792..8422)
                     /locus_tag="PLS88_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU05882.1"
                     /translation="MRMKHPWNAGWWFRPGKAEDAFLSDDWIGPSAAHSPKNKTIISK
                     EISQTDDAKNKTEDWKQIHLPHDMIEAPFNAFDERSFLRWGCYAKELKKEEILKITEG
                     LDKKTSINRYPAIFLSFEGVSVSCRVWVNGRLACSHKGPYTPFEIRIDTFIPHTWLEE
                     QSKEQGSKAERARPIWVLVEVDSAEDSGIPPFGGVVDYLAYGGIYRGVSLCADLGARM
                     SKLWAIPRPRGDALDGPWLVHISAEIEALESGSINNCSLLARLYLSGELVNEGNILLS
                     DSTRIEEKNKFSANFSMSVDRPKLWDIDSPALYDLELALFSEMGQELDYEQLRIGFRT
                     AEFGSSGFYLNQRRIFLRGLDRHQEYPYIGYAMGPDGQRRDAEILKEELGCIIVRTSH
                     YPQSPHFLDACDELGLLVFEELPGWQHIGGREWQEQALHDLEDMIVRDRNHPSIVLWG
                     VRINESKDNHEFYARTNELARRLDPYRQTGGVRCQARSELLEDVYTFNDFIYDGKGTM
                     YISEPAKVLPKYRKRAPYLITEHTGHMFPTKRFDQEERLVEHALRHAHILDTAMGNSR
                     VAGCIGWCAFDYHTHKDFGSGDRICYHGVADAFRIPKYAGLFYSSQISPSERIVLEPA
                     SIFAKGERNASHLLPIHVFTNCDAIDVYRSGGFVARFFPDKIHFANLPHPPIVIDDLI
                     GALLEAEGWPRNDLRLFRKLAGKAMSLGESSLDLWDKLRMGLFMRRHKLSIQDIEGLV
                     LRYGMNWGASDEKIRIVGILNGKEVVERSFGADSSAQKLSIESDTLWLGGLTEEEWPS
                     TRIVVKALDQYGNIVPFLFEPYSIEIKGPASLLGPAQRSLISGVSAFWISSKAKKGKI
                     RIAIACPRFKETAVIELDIE"
     gene            complement(8419..9555)
                     /locus_tag="PLS88_00565"
     CDS             complement(8419..9555)
                     /locus_tag="PLS88_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05883.1"
                     /translation="MMNERGEIPVEIVHLYAYDVGRSIDLNKVASLVPAHRDIALAKR
                     RDTPAYLTLPKPLVLSISTEECDSKQFSKISAMAKIYEDGAITVIVRYCTMSTFEELP
                     AKAHMPIRDVSGSSSIEQFAESSFRMVREALRSAIVGYKELSESDRETYIAFCLLECP
                     GGDPQAFINAHKDMAASLISGETAGTLHISQIEQILDKSFSYRRNDIAIFDLDRCLII
                     DPSADYDDVLMMAEHANYRLIELRELDFLLDEWLSEAERDIRRLYFSGRRKRSSERAL
                     RLKLAQIQGLRFDALFTLENLDNSSKIIGDYFLGSIYHRLCEIFNTDEWKFSVERRLN
                     ALQNIYELLKSDTTEQRMMTLEMVFIAVCIIFPILQIIQVMLVK"
     gene            complement(9552..10658)
                     /locus_tag="PLS88_00570"
     CDS             complement(9552..10658)
                     /locus_tag="PLS88_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOU05884.1"
                     /translation="MVKKDKKDIKDFTKILKETASKAPQKVSKAAKTVADSAGEQHEH
                     ISKWTKEQKRTFIWFALVMFLVMVIVAVLVFFLALRGEERTMVPDVRHMDLADALVKL
                     QQRELYPRLTLRFTDNPLDRNLVLEQSPPPGSIVKAGRRINLVVSRGAVLDRVEDYSG
                     RNIDDLKLYLQGISATTKTLVSIREPPLYIFDNSAPGTILDQEPKPGTEISGPTVLDL
                     VVSKGPESRNITMPSLIGLSMREMASKASTIPLVFSYKMRSAHEKETPGTVVEQSVQP
                     DTNLKQFDSVQITIATPEARKGYVSGVFEYSLPEYPYVVPVGLDVISPDGTRNSIYSV
                     KHPGGAFSAPFSVQNGSVLVLMVSGAEVTRKEVK"
     gene            complement(10738..11700)
                     /gene="fmt"
                     /locus_tag="PLS88_00575"
     CDS             complement(10738..11700)
                     /gene="fmt"
                     /locus_tag="PLS88_00575"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOU05885.1"
                     /translation="MRILFAGNPDIAVPTLESLHNSSHEVVGVLTNPVTKAGRGLKMN
                     STPIAQAALRLWGESVPILAFETLKSEARAAVSAVLPDILVSFAYGRIFGPRFMELFP
                     KGGINVHPSLLPRWRGSSPIPHAIMFMDKETGISIQTIAPEMDTGELLHVHRIPLKGR
                     ETSASLSELCAQLAGPMVVEVLDEIERGIAKPRPQIGEPTYCRKITKEDGHIDWHRPS
                     REIDARIRAFDPWPGSFTFLRNLRLSILEVEPFDDFDSMSNAGYMTNHSPGTIIAAVV
                     QKGIIVKAGDGYIAIKRLQVATRKAVGFQDFSNGARDIIGAVLR"
     gene            complement(11721..12209)
                     /gene="def"
                     /locus_tag="PLS88_00580"
     CDS             complement(11721..12209)
                     /gene="def"
                     /locus_tag="PLS88_00580"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOU05886.1"
                     /translation="MLNILTYGESILTEKANEITEFDQSLQRLIEEMHEAMQRDHGIG
                     LAAPQVGTSKRLFIVGIDDEPLRVFINPHIVASSQETTELEEGCLSFPGLYFTVVRPL
                     AVDIEAVDMHGKPFRISADGLLARVIQHEYDHLDGILFVDRVSPSKRKRALTHYGRML
                     KM"
     gene            complement(12299..12784)
                     /locus_tag="PLS88_00585"
     CDS             complement(12299..12784)
                     /locus_tag="PLS88_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOU05887.1"
                     /translation="MRKDTPTRYLSKSLIIIPFMAALILFTACDSGQGIDKPNPKLKT
                     VDLVIGTTKIKAEVALTEGERNRGLMYRSSLKDGEGMLFVFESDQKLSFWMKNTKLPL
                     SLAYILSDGTIVQIIDLVPYSEESRPSIRSVRYALEVPKGWFERAGIKVGDKVQIPSL
                     E"
     gene            complement(12916..13122)
                     /gene="rpsU"
                     /locus_tag="PLS88_00590"
     CDS             complement(12916..13122)
                     /gene="rpsU"
                     /locus_tag="PLS88_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOU05888.1"
                     /translation="MSQIIVEDNEPLEKALKRFKRMVEKEGIIREWKRREYYEKPSAI
                     RNREKKALERKLQKKTRRSQEGRY"
     gene            13255..14655
                     /locus_tag="PLS88_00595"
     CDS             13255..14655
                     /locus_tag="PLS88_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05889.1"
                     /translation="MLFSLGNLITLAVVIAMFVAYHLLTADNRSLEKLKRLGDKLKDE
                     LGSYVESKAEEVKHYGIDLDVQQKAAKVVLEKIQEAQATLDEKSDTLTEMAERVKKYD
                     NVLSQLMEMTKRVDQNIAHLAEKNDFIQAISRRLEDADKEIEIIEKEMPKLKEQFAHD
                     AQQILDGFRDDILDQLHEQLTGIVNLFEKSRSDAESAIVEATMLKEKIDEIATAALSA
                     AASRAHSIEDSAFATLRAHIANNFDEISRQTDARIEGLSKSIGELANAIEAKVAALST
                     DTASQLAESDRRLSEARAALNAMTNDMARIQKESTEITIALSKEFKAERNSLEEQFEQ
                     FGQAFEIHRASFEKEFLGEISALRESLEEAKAQSEELKSAAQGNIAMALDELEKQVTQ
                     ELESRRSALDEQIDEWLTSMDAKIRKVSQDALDQRKAEEARLAQEVNAELKRLKDSLY
                     TQTKKIERDIEAFKSV"
     gene            complement(14659..15363)
                     /gene="deoD"
                     /locus_tag="PLS88_00600"
     CDS             complement(14659..15363)
                     /gene="deoD"
                     /locus_tag="PLS88_00600"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOU05890.1"
                     /translation="MSIHIAAQKGQIADRILLPGDPLRAKFVAENYLENAFCFNEVRN
                     MLGYTGTYRGKLVSVMGTGMGIPSLSIYVNELIREYDVKKLIRIGTCGSMLEYLKVRD
                     IVLAMSACTDSAANHLRFKGMDYAPTASFNLLKAAWDAATARKLRVFAGSVLSSDMFY
                     TEDPEQWKLWARFGVLAVEMETAELYTLAAKFGCEALSVLTVSDDLVTGEITSSEERQ
                     SSFRAMVELALDAIFA"
     gene            complement(15543..16472)
                     /locus_tag="PLS88_00605"
     CDS             complement(15543..16472)
                     /locus_tag="PLS88_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05891.1"
                     /translation="MKRSFRFSSALLLISMMAMVFMVISCKQTKVVEKPLEDGASKTS
                     TKTDVTQKAIGTPGPHPEAGANLPEIKKQNENILIVYKHTGPGYMEAYGFDTYIFRLL
                     TDGSGQIVGGTSYQRTAGGEKEFAQYNASVSGDTISLAASSAEKKPWSYTIKTKGNRI
                     EISGAHNVVVTLDKSLVFSSPDGTYKEIYSSDPSEERLPSEIRKADAILEKGFWAYPE
                     KGKAVYNQTKPEDTENIEGFQITLWYQDKGDYRFATEGPEPLNEVYASGLAEALAGDH
                     GLINAVMLDLMLGESSYMRPIYAFGISRKGLGK"
     gene            complement(16670..17410)
                     /locus_tag="PLS88_00610"
     CDS             complement(16670..17410)
                     /locus_tag="PLS88_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05892.1"
                     /translation="MRTKKAIFIAACLLLGAVLQPFAFAVEGEAFVPVVRFLNLGEPL
                     DHDESAAVTGGDVRMSLNQDRTKLKVNVIDNEYEARLGRIPPKEVFEIDVHNRVVDTQ
                     KAPFMPADAAGRKLASSGSVTSKPSQFPEGYWSITAVTPREDKYGPYMISTNAVGKVD
                     TYVNDGKGNYKASGVYKDTGYAVHSNTNPFDASKSYGCLIVKQDDLSRVAGVLKQDRE
                     ENPKAVQKILVRPPVKKPHKLFGNRGDM"
     gene            complement(17483..18226)
                     /locus_tag="PLS88_00615"
     CDS             complement(17483..18226)
                     /locus_tag="PLS88_00615"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOU05893.1"
                     /translation="MYPRFEPSYIPDAHLIKELEDIRADGAFFASLDNDLEVFILRHG
                     QSEGNARNTFQGRYDFPLSVQGEKEARQAGEWLRQFKPDYILASPMLRAKRSAEIVAS
                     AINIDHIELDDNLIEVDTGIFSGLNPEIAAQRHPKIWDDFYKRSWDAIPEAESSQSIY
                     ARAIMAWQHIREIAARGASRIVCITHGGLLQWLIRSTLGVHDWLPLLPISNCCISKYE
                     IEIIKRNSIAFAQWTLINFHPLESSDRAR"
     gene            complement(18284..>18856)
                     /locus_tag="PLS88_00620"
     CDS             complement(18284..>18856)
                     /locus_tag="PLS88_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU05894.1"
                     /translation="AFVGTLLGFVIARKRNAAVRILDPLLMIPYMVPGTVLGIGFIVA
                     FNKPPIILAGTATIIILTYFIRRLPYSVRSAASILKQIDPALEEAGINLGSPPGRTFR
                     TITLPLMQGGIISGAIMSWVTSMNELSASILLYVGKTMTMPIKIYLSVADGYFGTASA
                     MSTILLFVTGIAMFIVNKYFNQGSETFIAA"
     assembly_gap    18857..18956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18957..19834)
                     /locus_tag="PLS88_00625"
     CDS             complement(<18957..19834)
                     /locus_tag="PLS88_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU05895.1"
                     /translation="MSQKAETPSIRHSRADIWSFVIFFGFVIVIVFLIYPLFDIFRYS
                     FLDKKTQTFSLSNWKTFFSKVYYLRAFWHSMVIAILTTFFSMVLGIPLAFFTTRYRIK
                     GTNLLNTLSVLALLSPTFIGAYSWITMLGRNGFLRLLFQSIGINLPPIYGPLGIVLAD
                     TLQYYPFISLMTAGALMTIDRSLEEASENLGARSIKTFFSVTLPLVLPSVTGGALIVF
                     MMSLSNFGTPMIIGGNYLVLPTLAYNLYTSEISQTPGMASTVSIILMLCASVVIVLQQ
                     WVSSRRKYASMLVNRPV"
     gene            complement(19831..20931)
                     /locus_tag="PLS88_00630"
     CDS             complement(19831..20931)
                     /locus_tag="PLS88_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU05896.1"
                     /translation="MTSIRIVDLEKRFGELRAISDINLEIEKGEFFTLLGPSGCGKTT
                     LLRTIAGFYKQDQGDIYIDGELINKVPAYQRNTAMVFQNYAVFPHMTVFDNVAYGLRV
                     RKLPKDEISRKVSAALKSVHLEGYEKRTPDKLSGGQQQRVGLARAMAIQPRVLLFDEP
                     LSNLDAKLRIEMREEIRAVQKSLGITSIYVTHDQEEALVISDRIAVMEQGVIQQIGKP
                     WEIYADPKNTFVAYFVGKINMLTMHLLEGAEGGLRSAVLGNLSLLVPSEGTEGLSEVI
                     VAFRPEDVIEAATEASQNKVMGILKTVSFMGSVARFELEVEGQEITIDRHRPRESDIP
                     QKGEHAFFAVPSDSMLLFDPKTGLRIGRGAQK"
     gene            complement(21121..22095)
                     /locus_tag="PLS88_00635"
     CDS             complement(21121..22095)
                     /locus_tag="PLS88_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU05897.1"
                     /translation="MKRFVVTILCLLLAVSAWAAGGKVVVYTAHEESIINAIVPMFEK
                     ETGITVEYVKMASGDVINRAKAEASRPQADVIWSIGGEQLEAENSILAPYTPKDWDKI
                     NPVYKVGTNWLPYTGIMNVFVVNTKMLTPDKYPKTWTDLTDARFKKNICSARADKSGS
                     SYMQLCNVIAIYGDKGWDIYKGIMKNTVISASSGAVPKFVNDGEQAVGITLEDNAFRY
                     FAGGGPVAIVYPTDGTVAAPDGIALLKGAPNLENAKRFIDWCLSKPIQEYLVDAMARR
                     PVRIDCKDPKGLPPLSTIKTIPYDFAWAAKNKEAFIAKFMDIAMELGL"
     gene            complement(22170..22781)
                     /locus_tag="PLS88_00640"
     CDS             complement(22170..22781)
                     /locus_tag="PLS88_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOU05898.1"
                     /translation="MYKAVVFDFGNVLCAVERENFIQKATIHAKAQNAQDLLRTLWGT
                     PIEIEFETGKIDSREYFRRVQSLVELEPSYSYEEFVRDYQSIIVPNPDGERALETAAS
                     LGARTFVLSNTSFLHASVIFDNEVLGTYPELYILSYKIGAMKPDPRIWMKLLEYIRLD
                     PKDCLYIDDIGDYCKAAQDLGMDAFCYDFRIHNLSLELQNMLQ"
     gene            complement(22859..23566)
                     /locus_tag="PLS88_00645"
     CDS             complement(22859..23566)
                     /locus_tag="PLS88_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05899.1"
                     /translation="MSQGKRHPFLARYYLILLVCGWIAIAILPLGAQDSYNAALSLFW
                     DGRRLEMAETRQAESQLAFQRSLAMTDRLLAADPSNPDLQTLKTWNLFRLHRYVDTVV
                     YAQSVLNTRQDHRILETMAEALFFLNKNEEALSAFSRYFAIAPESDDRRSSAYYYVGE
                     IYFRIKKYEHADIAFSTAVALEKNMYYWWYRLGLVKEVLGQYRRAYEAFNASLALKSN
                     FKPALEARERVKAKSSL"
     gene            complement(23586..25040)
                     /gene="gltA"
                     /locus_tag="PLS88_00650"
     CDS             complement(23586..25040)
                     /gene="gltA"
                     /locus_tag="PLS88_00650"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOU05900.1"
                     /translation="MEDAELELSRLRSGPITPKMRMQIPPQEMPTQDPIERRSNMNEV
                     ALGYTSAQAMLEAERCLQCKNAPCIAGCPVRVDIPGFIGKIKEGDFLGAARIIKRTNL
                     LPSICGRVCPQETQCQAPCTVGKALHSVEKAIQIGRLERFAADYERGHGAVEIPHVSQ
                     RTGRKVAIVGAGPAGLTCAVDVRREGHSVAIFEAFHKPGGVMVYGIPEFRLPKVLVQA
                     EAEVLQSMGVDFELNFLVGRTRPLLSLLKEDGFEAIFIGTGAGLPKFMDIPGENLVGV
                     FSANEYLTRTNLLKAYMKGKAGTPIYPSRRVAVLGGGNVAMDSARMALRLGAEEVRVL
                     YRRTRSEMPARAEEVEHAVEEGIIFDFLKSPTRVIGDDKGRVAALEVLSYELGEPDES
                     GRRKPVPIHGSEEIIPFDTVIVAIGNESNPLISKTTPELQVDRHGHIIVDERQKTSIE
                     RVYAGGDIVLGSATVILAMGEGRRAAQSINEILQ"
     gene            complement(25061..25948)
                     /locus_tag="PLS88_00655"
     CDS             complement(25061..25948)
                     /locus_tag="PLS88_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOU05901.1"
                     /translation="MHNVVSKVQLSEEVFRIEVEAPLVARERKPGQFVIVMCDDEYSE
                     RIPLTIADADPDRGTVTLIFQTVGASTHKLALKNPGDKVILLGPLGNPTHIELYGWTV
                     CVGGGIGLAPLYPIAKALKQAGNRVTVISGARTKDLLIMQNELAAIADEHIVVTDDGS
                     FGRKALVTEPLDELCRRDPPPDLVVAIGPPIMMKFCAETTRPYGIKTLASLNTIMLDG
                     TGMCGGCRVSIGGKTKFVCVDGPEFDAHLVDWDGMMIRLGTYRDIEKEAHDRCHLELQ
                     LEKLEAEELTASTLKESAK"
     gene            26145..26957
                     /locus_tag="PLS88_00660"
     CDS             26145..26957
                     /locus_tag="PLS88_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOU05902.1"
                     /translation="MSLPIVSQKLEDEALFKPLDFLAYLKKTGIALSHRPAPDAIILS
                     YQKSLSDFVQATYPCERAKGYFGHFVHYLDLSKADIPDKDSLISNKIIAISSDFGVGA
                     PAAAVMLEELIAWGVREFISIGFAGSLREDLPPGSLVVCDNAFRDEGTSYHYIETTAP
                     ATPDPRLTSKLESCLASTGLSYTKGPTWTTDAIYRETPSEVQYFREQGALVADMEAAA
                     LFAVAKYRSVPIASCFSVSDTLAYLEWRPEFHAEPTMQGLEMLFSCAVRALK"
     gene            complement(27004..28650)
                     /locus_tag="PLS88_00665"
     CDS             complement(27004..28650)
                     /locus_tag="PLS88_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOU05903.1"
                     /translation="MKLLVLAAGIGSRFGGVKQVTGVGPNGETLLEYSIYDARRAGFN
                     EVIFLIRPEIEADFRSNVLSRLPSDIRYSLAYQTLDSLLDKALIEIYGENEENAREGG
                     LGESKRLLMSKRVKPWGTAHALLCTREFFVQGETFAIINADDYYGRRAFEAVGSHLFH
                     NPEELCFAGYKLEDVVPASGTVSRGICTLRDDGYLEKIIEHKKVWREGNCFYSRQGES
                     IVELSPDTYVSMNFWGLNSSIFDYAWQGWLEFLDRYIESPTQEFLLPDLIQSLVEKKG
                     ARVRMLAASELSFGLTNPEDLQEVRQRISRLVAEGVYPSPLFNNQKIESFPRLWRFEP
                     NEYRECAEFEDLFAQVNVPWDVLRLLDSYLRSKLDAVSGERIRSSVPKGVHIEADVFI
                     DEGCIIEEGAFIRGPAWIGKGCEIRSSCYIRGGLLAGSGVVLGHASEFKHCILLERAQ
                     APHFNYVGDSVLGHHAHIGAGVILSNFRLDGKPVRVKSLDSQEKIETGLAKFGALIGD
                     YCEIGCNSVLNPGTILGEKSVVLPMSPVSGTWPAESRVHT"
     gene            28891..32451
                     /gene="nifJ"
                     /locus_tag="PLS88_00670"
     CDS             28891..32451
                     /gene="nifJ"
                     /locus_tag="PLS88_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOU05904.1"
                     /translation="MTNTKNMVMIDGNAAAAHVAHACSDVIAIYPITPSSPMGELADE
                     FSSMGRNNIWGTVPQVVEMQSEAGAAGTVHGALTTGALSTTFTASQGLLLMIPNMFKI
                     AGEAIPTVFHIAARAIAAHALSIFGDHQDVMATRQTGWALLASNNVQEVMDLALIAHA
                     ATLKARVPFLHFFDGFRTSHEVSKIEELSYDTIRAMIPEEKVREHRARGLSPEHPVIR
                     GTSQNPDVYFQGRETINKLYEATPAIVEETMKQFAELTGRAYKLFDYVGAKDAERVII
                     LMGSGADTAEETAEHLVKQGEKVGVIKVHLYRPFSVEHFIKTIPATVKSIAVLDRTKE
                     SGSIGEPLYEDVRTSIGEAMGNHSAPFNNWPTVVGGRYGLGSYEFTPAMVKAVFDNLK
                     LEHPKNHFTIGINDDVSFTSLPYDEEFHLPGDGIVQCLFYGLGSDGTVGANKNSIKII
                     GDETDNCAQGYFVYDSRKAGTYTISHLRFGPKQINKPYLITKADFLACHKFSFIERID
                     MLANIKVGGTFLLNSPYPADEVWDNLPIEVQKEIIDKKLSFYVIDAMAIADKAGMGTR
                     INTIMQTAFFKISGVLPEEQAVELIKKYTKKTYMRKGADVVQKNLDAIDLALNATHKV
                     KIGQANSTKHMLPPVPDTAPKFVRETLGEIIAQRGEKMPVSKLPLDGTFPTGTTQYEK
                     RNIAEKIPVWDPSLCTQCGNCSLVCPHGVIRMKAYDPEILKNAPSTFKSVDGKGKDVA
                     GLKVTVQVAPEDCTGCGACINICPAIDRANPGRKAINFGSQIELRDTEKQNWDFFLSI
                     PETDSKFLNLSIPKGIAMKRPLFEFSGACAGCGETPYIKLVSQLFGDRAIIANATGCS
                     SIYGGNLPTTPYTKRADGRGPAWSNSLFEDAAEFGYGMRLTSDKQNEYAQQLLASAKG
                     KGIPEELADRLLSNTQESDEQIEAQRVDVAALRECLAGKTEGWAKELDNVAEALIRRS
                     VWIFGGDGWAYDIGFGGLDHVIASGKNVNLLVLDTEVYSNTGGQASKATPIGAIARFA
                     TAGKETSKKDLGMIAASYGYVYVAQIALGANMVQAIKAIREAESYNGPSIVIAYSHCI
                     NHGIDMMKGMDQQKMAVQSGMWPLYRYDPRLKAQGKNPFQLDSREPDYSKLEQYMYAE
                     VRFKSLVQASPERAKVLFEKQRDLIERRYKEYRYLADRPF"
     gene            32578..32940
                     /locus_tag="PLS88_00675"
     CDS             32578..32940
                     /locus_tag="PLS88_00675"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOU05905.1"
                     /translation="MNTIKSYKWTIASAIAVLVLLLIPSQAFQKAPQSLIGLDKFVHA
                     ILFGAMTAIFCAEHRALKKMNPKFLLSLIIISAFAFLTEISQLLTITRQFDLKDFGAD
                     TIGIATALVVMRIASLLS"
     gene            33014..33820
                     /locus_tag="PLS88_00680"
     CDS             33014..33820
                     /locus_tag="PLS88_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU05906.1"
                     /translation="MENIEKQNSSAQKNDATHATEKFSEIDSSTMLHLQKSSFLFFLT
                     TSIFLVFCFILTTLMPGIDFLSFYPLEMLILMGLLTFGFLIIHLNRKLAWLEPALVIA
                     ILPLTLLKYSTSIYSMCIFIIAVIELSRLGYFRRATTIKACILVSYYALSLVLIAIAH
                     REFFLDIIMSIIFLSLFFIYFLPIIQDRWQIGKTNPIYKINLRDLNLSCREMDFLRDC
                     LHGATFKEIAYKHHVSESTVRNTFAHIYHKFNAADRMDLLSKLAAFDIIE"
     gene            33837..34748
                     /gene="mazG"
                     /locus_tag="PLS88_00685"
     CDS             33837..34748
                     /gene="mazG"
                     /locus_tag="PLS88_00685"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOU05907.1"
                     /translation="MDLDLMPLRDIAGENADSILADADKTAKAFKYFYAVIARLRASD
                     GCPWDLEQTPSSIRGNIIEEAYELAEAITEDDNPHIKEETGDLYLLATMVGYISQQEG
                     RFSVADSLCDEACKLIRRHPHVFGESDVNSPDQVVQQWNEIKEQVEGRRKKESILDEV
                     NRNLPPLEKAYKLQKKASKVGFDWQQKQDIWNKLEEELEELKEARVIFSEECESAKAV
                     DNISRKDALEEEFGDLLFTVINAARLYGIDPSIALHRTNEKFSRRFRHVEERMKKQHL
                     EMNQENMNKMDTFWNEAKKGEIIGKNN"
     gene            34805..37033
                     /locus_tag="PLS88_00690"
     CDS             34805..37033
                     /locus_tag="PLS88_00690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU05908.1"
                     /translation="MANLLSAQKLAKARKTFNIYNFLNSFSFVFVSGSIITLFALKLG
                     AQNSIIGLLNALAYVTFFMLPLGKRLVKRSSIIKVFGWGWVVRYIALVPILFAPLFAS
                     KGHMGLAILMLILGTAGFHIARGIALIGNNPVLGLLSTGGGDKPRSDQGRYLVTISVV
                     NSVAAILANIVLAFLLGQEASLWTYTIAIGLGIITGLISCAILFKTPEPEAFSSKEEQ
                     SLWDVTKEALVQPEFRAFIFTFMLLSFLSGMGRAFLPVYAKEVFAQGDDAIMVYSIVA
                     SLGSITMGLITRLVVDRLGSKPLFIIYSAIGFVSFLPIALIPDSATLMGAPSVIALFL
                     AIVHFLSSFGFAGEENAAQVYYFGLVPKEKMLDLAVIYNLAYGIGGSLGSFLGGGILE
                     IFSNIGLSNAGAFRLYYAAISIGLVVVIYSMRNLPHMGAKSVTQSISILLSPKELRAF
                     DLLTRLDQSASAHEEVRIIQKLSKSESNLTQKELVEYLYSPRFEVRSEALLALESIPE
                     LDHETIAALIREVKANVYTTAYVAARILGKQRSSIAIPALIESLDGPDYMLQSMAMVA
                     LARLEAFEAIEKIERLIQSTNNPRVRISGAYALELFGRINSIPTLISCLKAEDRPANV
                     SDELVLSISGILGIDKEFYLLYSEFIDDNSSGIAQLIAYAEDLNLGDDRLNAWISAVD
                     ALFAPDSDTAPLARLLLASPKITDSILMLSDGLLDPKLTYPGFNFLCAYIALHIEDLK
                     DY"
     gene            complement(37066..37659)
                     /locus_tag="PLS88_00695"
     CDS             complement(37066..37659)
                     /locus_tag="PLS88_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOU05909.1"
                     /translation="MVFSTLAKIVSSLVSLYMVLCIIRIFFSWIPSLTDTKWGKLIAR
                     LTDPYLNLFRGFSLFRTTAVDFSPIVALAVLSVLNNLFMTLSYAVRITLGFILSLLLD
                     AAWSAISFVLGFFLVISVIRIIGFLARIAVLHPLWQILDGIINPLLFRINKIIYRGRA
                     VQYLQGLITGFVAVLVVRALGGLLVRLLTSLLMSLPI"
     gene            37737..38528
                     /locus_tag="PLS88_00700"
     CDS             37737..38528
                     /locus_tag="PLS88_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOU05910.1"
                     /translation="MHIGNVHIGTCGFSYEEWKTVFYPADLPSNEMLRYYSLVFSFVE
                     LDNTWYRMPNPNQLQRLALITPSDFRISLKVHRSLTHEIDESWQTKADEFSLAVSALS
                     STGRLGCVLVQLPYRFSYTIENRKYLANLLAALSNFPLVVEFRNATWYQDRVFETLRK
                     LKVGLVTVDRPDLPGLPPESVIITSDICYYRLHGRNTDLWWSGNAKSRYEYCYSEQEL
                     KDRARLIRAMAKQSKTVFVAFNNHSSGYAPKNAAYIQSILAEQQL"
     gene            complement(38542..38982)
                     /locus_tag="PLS88_00705"
     CDS             complement(38542..38982)
                     /locus_tag="PLS88_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05911.1"
                     /translation="MRKPFEISTGAWWVIADGRTIAVPSFHESWLASHPGIAGGALHT
                     IDFVEKSGWLSVTLYSEGLIEVISRDILDNRQREALKQLLEINRSLIRKMVLFVPSID
                     GCFTAEDSTLDDWEQLSKQIEAFAAKEIKKDLSEESIELDSNQP"
     gene            complement(38966..39787)
                     /locus_tag="PLS88_00710"
     CDS             complement(38966..39787)
                     /locus_tag="PLS88_00710"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU05912.1"
                     /translation="MIRILFEDDEIIVIDKPAGLAAQPGQGLRDNVIAALERQLGYRA
                     FPVHRLDRETAGCMMLAKDAKAANKWSNRIASREITKRYQAWVTGVPSKPKGTIEDAL
                     EGKRGAQAACTIWTLRETWLFPLQGPPSNNPVDTIGKEHVRNEIMLANQYPAHVQVDA
                     AEANFLTLSLLELELSTGRMHQIRRHLAGIYLPVLGDDKYGNFLLNKALRKKGIRHLM
                     LWAYELVLPWPSLKPPNMPILAAEPPHFISLREILEREGRILDKRYTEQSDEKTI"
     gene            complement(39913..40761)
                     /locus_tag="PLS88_00715"
     CDS             complement(39913..40761)
                     /locus_tag="PLS88_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOU05913.1"
                     /translation="MNQGALDTIEAAIPNLAEAERKIAVFVQESPQKALHLNISELAR
                     QAGTSPAAVVRFCRHIGVGKYSDFKLWLAKDVYQGWGEKFLPDLELESQTPGARAIRD
                     MTEAVRRTMNSLAQTLDPIVVEEAADRIRGASMTAIFGIGASGLVAEDFHQKLTRIGI
                     PSSYLFDTHAQITASCSLRQSDLAFIISYSGETNEMIEVAAQAKTRGCFLISMTMTGQ
                     NRLRDYSDLILEIPTVERIYRSAAELSRISQLAAIDILFNVLISYDLDSAISALERSM
                     QATHHF"
     gene            complement(40764..41723)
                     /gene="murQ"
                     /locus_tag="PLS88_00720"
     CDS             complement(40764..41723)
                     /gene="murQ"
                     /locus_tag="PLS88_00720"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOU05914.1"
                     /translation="MDIDSVDEYLERATTESRNVHSEHIDDLPVIEILRIINEEDKGV
                     PFAVEKALPSIAALVEDVVRAFNNDGRLVYIGAGTSGRLGVLDASECPPTFGVPPSMV
                     VGLIAGGDAALRNSIEGAEDHPENGVKALKDIDFSAQDVLVGITASGAAPYVLGAMEY
                     ARNLGAAVAAISCNRQSKTFEIAHHKILIEVGPEVITGSTRMKAGTAQKLVLNMITTA
                     SMIRIGKVYGNLMVDLMPVNRKLIARSKRLIRQATGCSKDEAEAAFLASGGKPKIAIL
                     MTILGIGADQAAELIERGDGQLSEAIRIYSKDKGESDQKNKGE"
     gene            41908..43152
                     /locus_tag="PLS88_00725"
     CDS             41908..43152
                     /locus_tag="PLS88_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU05915.1"
                     /translation="MAKKLSIVLLLIAMLSPMYAQQKTVSIWGWRAQDVDVWKGVEAA
                     LKAKGEQISIRYEVFPPTEYDSKLLVSLQGGVGPDIMYTRRLPGARTQALLDNGYLTA
                     INGLVDLSNFDDVSLSFISTDNKVWGVPFANQIIGIFYNKAIFDKYKLKEPQTWDEMV
                     QIAETLKKNGVTPFFVTGKEAWILAMQNAMVGVSYPGDEWIGKLTVGKAKFTDPEYVN
                     MLKDLNALKKYYQKDFMANTSAEQDVAFAMEQAAMVFYGVWGNTNWLKMNPDLKFDYF
                     PIPPKDKNVPARAYTYMDGAYGLNNTSKNKEAALKVLNYTGTKTYGELFSSTTGEITA
                     IKGVTMPASKPILVKCYEKMTTIAAKNRYWVGSPFDAGTPSVYNILEKDMQSMYLDQM
                     TPEALAKRLQDGISTWYPAFKK"
     gene            43284..44183
                     /locus_tag="PLS88_00730"
     CDS             43284..44183
                     /locus_tag="PLS88_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU05916.1"
                     /translation="MRKYSFLWMLLPAFILYSVFIAYPFFSSLGLSFYDWPGIGPKKF
                     IGLANYKNILSGFMSSEFYNALWHNVVFFIWSLILSVVPGLFFAFLLAANMKGTGWLK
                     VVFFFPNTLAIVVVGFLWGLLLNPQWGLINQLFRVVGLDFLAKPWLGDTKTALPTIIF
                     VTAWRGLGFYILIYLAAILGVDREMIEAARIDGASELQIASRIILPHLYSIIATTSML
                     KFIWTFNIFDIVYAMEGTQGGPAGSTDVLGTLFYRIAFGGLGASQVGMGLGATVVTLI
                     FLIVLPVSIFYVFIVEKRVEKGE"
     gene            44183..45010
                     /locus_tag="PLS88_00735"
     CDS             44183..45010
                     /locus_tag="PLS88_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU05917.1"
                     /translation="MLKFNGFQKVLAWIFLLLLAFANLYPIGIMMLSSFKSTKEIFLE
                     PFNLPSVWRWQNYVSAWQRADFATYFKNSVLVTALSIVSIIIVSSMAAYVIARFDFKF
                     KRGVYLYMLAGLALPTRLAIIPIFLIMRNLHLLDKLSGLVIVYTASGIAFSVFLLVNF
                     FKNLPRDIEDSAKIDGAGPFRIYWQIDLPLLKPALVTVAMFNFIDVWNDFFFPLILLS
                     SKAKKTVPLGLQAFFGEYTVEWDVLFAALNISVLPILIVFLILSKQFIAGLTEGALK"
     gene            complement(45077..46261)
                     /locus_tag="PLS88_00740"
     CDS             complement(45077..46261)
                     /locus_tag="PLS88_00740"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOU05918.1"
                     /translation="MIKSKQQQASVVFGLEHFLDDPRRYNKRYGLATNPAAITSRGIP
                     SWKAFMEHGIIPVCLFGPEHGFRGAAQDAVELDDEVFRGIPAYSLYGKRLRPEPWMLE
                     SLDALVFDMQDVGCRYYTYLYTLAYIMEACAKVGTALIVLDRPNPIGGSKVEGSPIPK
                     ELESFVGGYGLPPRYGMTIGEFALYLQGEYYPNCRLELIEMSGWDSRPAWIETNLPWP
                     LPSPNLPTLSCAELYPGTCLVEGTWLSEGRGTSRPFEIIGAPFIDGEALREQLAALEL
                     PGVVFISLFFSPTASKYKGELCEGVLISLTDREALRSLDTGIALIHTINRMYPNEFRW
                     REDWDDKELSFFDKLAGGTTLRFMIISGAPLEDCLEFAHRGEKEFLRIREKYLRYGTQ
                     KG"
     gene            46285..47469
                     /locus_tag="PLS88_00745"
     CDS             46285..47469
                     /locus_tag="PLS88_00745"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOU05919.1"
                     /translation="MTYNSNKGSQKSHNQAEETLENLLDNAIAKDLCTGIAASVSIDG
                     RLLWSHCSGFTAREELALSKPIENDGKTSNSIIAPIDPETRFDVASMTKPLSTSLLIL
                     KALQAGALNLQDSLGKYLPEVNYRTGQIPLLALLTHSSGLPAIPALHSFFADSGRLDR
                     SLALERLFSIEPQFAIGKHVVYSCTGYIFLGALLERISGMLLGELFQKEIAAPLKLEQ
                     ASFAKKIQKSGEPEHLDNAVATEYCPWRNRRIQGQVHDESSYCLGGHSGNAGLFLSLK
                     DAITLGNFIMQEGTFGGKQILKPEIIQLLWREYTHGLEERRSLGFKLHDSTTVDGPLW
                     PLQSFGHTGFVGTSVFFEPQRRLFAILLTNRVYFGREQTADSILAFRREFYSRVWHAF
                     AE"
     gene            47738..49042
                     /locus_tag="PLS88_00750"
     CDS             47738..49042
                     /locus_tag="PLS88_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU05920.1"
                     /translation="MKKAIALCVLAIAILASVPAQAPVVPGLGSKKITINLWTHEDPN
                     RSVIEKKYIAEFMAANPNITVNYNTYPSNRIFEALTTAFAGKQGPEIFNVSIGNAVSF
                     IDNGRVAPVDYKALGLKGPADLEAMYLPGMLKAVERDGDFYGLPLEITNMCLYVNRKM
                     LKEAGIDPDKDYPKTWEDLISLSEKIVKREGQIITRRGFDFRYDAYTNLFIPMVEQLG
                     GEYVSKDGKKAIIGDEAWLKALQWMKELGPAGKNLGSPTYTAARTAFDKNDNTVAMSF
                     SGLYQEARMKASNPDFYNSKDWMVVPFPQWKDSKKKVTANYSAHYYMVNAQSDKLAQQ
                     ASWVLVGWMLSHGEEYLDKVAIIQPTKKLIESPIYKSMPYSDVFAKDFENANIVYVGK
                     ASARLSTLLSETIQNVMVGGVDPKVALEKLRKSAQEALDSAD"
     gene            49087..49992
                     /locus_tag="PLS88_00755"
     CDS             49087..49992
                     /locus_tag="PLS88_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU05921.1"
                     /translation="MGDRRNPLEEKRNRWGWFFVAPGIVFFSAFSLYPILYAIWTSFH
                     NKKLLSLKPPKFIGLQNWISVLSSQDFWNSVRASAVFCIGVFIPVFLGSLLFALLISS
                     RKKGKRFYQMALYSPAILSSVVTASVWLLIFDPRGLANQFLNTLLGTAGVDHKWLADP
                     VMVQLSTILVYVWKQVGYYTILFGTGIGKIPSSVVEASMIDGANSWQRLTRITLPLLR
                     PTMALVSVMIFISSLKTFSTQYLFNQHGAPLAPINVLTLNIYRTALSDQNLGKASVMS
                     LFLFFILLVLSWLQVKAGGQGEELE"
     gene            49989..50867
                     /locus_tag="PLS88_00760"
     CDS             49989..50867
                     /locus_tag="PLS88_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU05922.1"
                     /translation="MKHETSKLSSRWNTIKYNAGSFIATAILVIACSAAILPFAFMLS
                     ASLMPGHDVTTIPYRWIPETPLWSNYLRALKGSDGSWIYLRNIANSFIVASSVALSSV
                     ILASLTGYGLAKFHFKGRNIVFMGIMATMMIPFETVMIPLYLTAVKFGLQNRLIGLIF
                     PFLTSAFGVFLMRQFLLGFPDELLDAARIDGCSEFQIYRSIVFRNATPAIATLAILAF
                     RGQWDNLIWPLLIIQKEELKTIPLYIVLFTSELSTDEGAMMAVAVIAALPVFILFFTM
                     SKYFLSGAGVYTGSKI"
     gene            50864..>51357
                     /locus_tag="PLS88_00765"
     CDS             50864..>51357
                     /locus_tag="PLS88_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU05923.1"
                     /translation="MIDLYIFDEGGVLIRNHMIMGDVAAALGLDITTFLEFIAPDIKS
                     LSRGSIDSAEFWRRFSDRTGIRPSENYLKTLFRPLPDEATFSLVNELSKKARVVCGTN
                     TIDSHHEINLQLGLYSPFQAVYASHIIGHIKPELDFWQHILAEEGVSAQRTFFTDDSE
                     DNIKA"
     assembly_gap    51358..51457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            51636..52580
                     /locus_tag="PLS88_00770"
     CDS             51636..52580
                     /locus_tag="PLS88_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOU05924.1"
                     /translation="MNNQWIFGIDGGGTATRLRIESLEGKVLYQAEGSSLNPRSVGWN
                     GSRKTLDDLFGSMYKSTNLSTDECISGFAGVAGIDREADTGTMRSIIREAGNLAFDTL
                     IEVKNDSIPALAGAFGELRGILLIAGTGSIAVGADGAGRIIRSGGWGHILGDEGSAYW
                     VGLRALNAAIRFQDRRGPQTDLLGRALAFFGEKEAFSLIPAIYEDFDKAKIAAFARIV
                     AEEQDRGDEIARQIFIEASEELALLATSIAIRLGEAHSGGRIAFSGGFITNNEQLWRD
                     TEARILSALPRHRVVVPQADATIGACILARSIANAAAR"
     gene            complement(52584..53450)
                     /locus_tag="PLS88_00775"
     CDS             complement(52584..53450)
                     /locus_tag="PLS88_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOU05925.1"
                     /translation="MKTRRFKASFFGVIVAVIWGMTFLSIKVALREFGPMSLPLFRFT
                     IASFLLALIMLLTRTSFRVAWKDVPMLSLSAFTGVTLYYYFENNGIMRLTASESSLIV
                     GTIPVVTLLAEIIAFKVRPRRSVILGIVLSFAGVALIVMRSGNASTSVSGYFFMLGAV
                     LSWVAYGFITNPLSGRYPMLTITFWQMVIGALGCVPFALAEHQSWTGFSASVLLNATF
                     LAVFGSAIGYWLYIIVLEELGPGRSSVFINLIPVVSVTASFVFLGERLSAIQIAGACL
                     AIGGVYLASSSS"
     gene            53487..55103
                     /gene="hutH"
                     /locus_tag="PLS88_00780"
     CDS             53487..55103
                     /gene="hutH"
                     /locus_tag="PLS88_00780"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOU05926.1"
                     /translation="MGGTSYAILESMNEVIVDGCSLSISAVVAVARHKFPVRLDPDAE
                     LRMQASREVVEFAVKKKQVKYGLTTGFGKFCNVIISEEDNARLQKNLIMSHACGQGEP
                     FAPEIVRAMMLLRANALAVGNSGVRPVVVQRILQLINEDIVPIVPEKGSLGASGDLAP
                     LAHIALVLIGMGEALYKGAKMTGARALSEANIQPLVLEAKEGLALINGTQAMAALGAL
                     SVYDALLLYRSATSASALTFQALRGITEALDPRIHELRRQKAQIEAAQDLRRLLEDSR
                     LTSAASGTRVQDAYALRCIPQVHGACLAAIKHVWEIVSAELNAVTDNPLIFPYVLREG
                     GDIISGGNFHGEPLALALDYLGIAASELANLSERRLERMVNPTLSEGLPAFLTINGGL
                     NSGFMIVQYSAASLVSENKVLSHPASVDSIPSSANQEDHVSMGTIAARKARQIIDNTR
                     RVVAMELLAACQALDLRAIQIGLPSIVDSLSPATAPIYGIIRTVVRFAEQDRIMYPDI
                     DAVVRLLSDEILPQTMERWSWNPEPVVAHE"
     gene            55161..>58008
                     /locus_tag="PLS88_00785"
     CDS             55161..>58008
                     /locus_tag="PLS88_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05927.1"
                     /translation="MVTKRDHGLRLDRTSPQERARLISYINIKLKSLGLPVYSKEGIG
                     FVQLAADMLESFRQKNRLLPKILPPADQRIQNFIDQYLADLGLARIPQLPSNTLVLDH
                     YGMARELSLPPDGPKHVSPTLTSYRVRNGVLHNPKSDRRTTEGVFHIAEGGLPIPLDK
                     KAVPKPCFAKLLEAALNPPLDMMELPFTAGENEKAHTFASVLLRPIVRPEIPDYCEER
                     SMEIRFFAPGSLVANIDFVESIFGNSGDPFIPENDAAIDPVHWTGTTGCIILATHLTE
                     LTKKELGLPTWEQATERQRRDGMCWHDPAEKYNDGKPFKITARDESGVIITIIADNYF
                     GYSKKEIKSHISYSANLLGLAEEEHAGGALVFPSYNLGTRFVPDTNLKSKGHSLNDVY
                     SIMRGRIDIKQEGYAIDFTFPNIIYLPEDAYISLEDQMAHWVSDGIEESLRILPGNIY
                     IHPTGYQIRMEQHSVAGSWRLIGTSAEGLLCHKPCTVSGGGKSEIAKSIQDAIVYMPI
                     VIADFDKDMQEVKKIIEKDYGNRFRDESDNLGKDTRPVLSPKRSLGSVIKLLSPSPAY
                     SDEYNEWLRSIPERIKSLVFLVKRFYRPDWGLDWMSHFSVDAVNGTTGNILKFEGKPI
                     MGSYLRVGKNEKGLNRFFKLRQDFMPAFKLQWEDDITASIIVPVNQLENLPDWASDHM
                     SLKFAKNCEARFFQRPDDAIIRGYDKQTEKDLTRHDNFLSNFEPLTRADASRIIENTI
                     HFSEYTEPMRKFIEDSEKDPDFEYFVASNYFRIVDDLPSKNPRYLQLDPSYADPMARY
                     LAELGPRLYRRIPADKPLPQPIGAVLPGRRNNPPDRQAGIRPLAVYGPIHYQELPELF
                     MDFVCSLTGKSPSTTGAGSEGALTKGPFNALVPTTDLNNALLGFILTGYAGFTTAAGH
                     IGHKYKIDHDISLMMPELWSRLSPEERDPEFLKKN"
BASE COUNT        15567 a        13839 c        12802 g        15600 t
ORIGIN      
        1 aaaccatgct catcatttgg aatctgacaa acatcccgat cttgggatga ttcacctgaa
       61 ataactctct gtatcacctc cttttcaaat atagggccca ttatatcact attatcggag
      121 caatggaagc caatattgag catttccagc tcttatatgc ctgcttttat ggaatgcgtg
      181 ccacgatatc cttgagccag ggctttttct cgagatcgcg ctgcaggcct tcggctcgcg
      241 ccctattcac ataatcgcgg ttttcgaaat agtagcgata attagtatga acatcgtagg
      301 ttccattgat aagcgctatc gtgtcttctg tcatgaccag ttcttcatcg gtcgggatga
      361 taaagatctt gaccttcgag ctggaccctg taatgtcgag ttctgcgttg cggcacatag
      421 caagagcgtt tttcttctca tctatagcta ttcctagctc ctcgaggcct tgagttgcca
      481 gattgcgtat aacagggctc atctctccta ctcctgcagt aaagacaatc gcgtctacat
      541 gaccaagagc cgcgtagtat gcgccaatgt atttctttat tcggtatgct tctacctgct
      601 gtgccaagat ggccgcagga tcgccttttt ctgctgcatt tgccacatcg cggcggtctg
      661 cccattttgc ggtaatgcct agcacacccg attctttgtt tagctttttc tcgatctccg
      721 cagcggtcat cccggttttt ctcattatgt gaaagatgat tcccggatca acatcgccgc
      781 ttctagttcc catgacaaga ccttcgagtg gggtcaagcc catggaagta tcgtaagaac
      841 aaccgttctt tacggcgttg atagatgccc cattgccaat atgagctatt acaagatttg
      901 tctcgaatgg gtctttgcca agcagcacgg ctgcgcgctt agcattgtag agaaagctcg
      961 ttccatggaa tccatagcgg cgaaccatgt atttctcgta ccattcgcgc gggagagcat
     1021 acatatagct cgaggctggc atggtttggt gccaggcggt atccataatt gcgcattgcg
     1081 gcacattggg gagtattgtt gtggccgctt ctacacccat gaggtttgct ggattatgaa
     1141 gaggcgcgag ttcgttgaga gacttgaagg tttccaggaa ttctccattt attattgaag
     1201 atttggcaaa cttcgagcca ccgtggacca tgcgatggcc aaccgccttg atcatcgaaa
     1261 gatcagaaat aacaccgact tctgggtcga caagagtctt tagaattagc tcgatggcta
     1321 ctacatgatt ggggcattcg tgctcgacct cataatcggg cctgtttcgg actttgtgag
     1381 tgataaagga gccaccgata gtgacacgtt ctacgattcc atttgcgagc acatccttct
     1441 tctcccaatc atagacctga tatttgactg aagatgaacc acagttgagc gttaagataa
     1501 ccattttaag actcctgagg agatataatt tcagccagta tagcactgtg cataggactg
     1561 cgcaataaat taacaaaatt gcaaaattac ctttgcacaa aattattgtg catcctaaaa
     1621 gcgggctcta tttcgaaatt tctcagccgc ctacgacaag ctcaatggcg atcatggtaa
     1681 caatgtacat aatcggcgta atccggccga taatgcgatg tgttttcttc aagctcgcga
     1741 ttctttgctt atcctttgac ttgaaaatcg aaaatcccag aattggctcg attatgagca
     1801 ataccagggt gagcagtcca aggaggtgat gcacaagcgg caactgtact ccatacccag
     1861 gagttaccat gataatcgct actataattc caatgaccgc gagcacagga gcagcgatgt
     1921 tcaatcctcg gtgcaatttg agccaccact tcttgcttct gtagtaatgg gctatgataa
     1981 tagcgaccac catacatact aatccaagtc ccatgaacgc gccatgcaat gtaattatat
     2041 tcatgtaaaa gccttccttg attcgcacaa aattgcgtat cgatattatt ttgccatttc
     2101 gacagtggct gtgccagcct tcttgtgttt cgcagtcatg tcgaggctag ggccgtaagc
     2161 ccacataatc ttaaacttcc ccgaagtggg aatcgcttta tcgtatttat cttttgtatc
     2221 cagaagccga gagaattcaa agattacggt gtctcctgac cgtgaaccag catatgaaag
     2281 aacatctgag gttcctccct tttcaatgtc tggcgcatga ggaccgaaca tgccagatga
     2341 ccactcatca tagactttga tatcctgact atcctttact accgcaagga tcaggtcgga
     2401 attggacata aagctcgatg gattgaatcc caccgagatc catccagggg tcttagctat
     2461 aatcgccatg aatacttttt cgccttcaaa ttcccataag agcttgaaat tccctttatc
     2521 gaattctgtc tgtttggaat attcgcccga agagattgcg ccatcgattg tgatggcgaa
     2581 cgacgatgga actatcacaa agagagaaaa catgcaaaat atgacatatg ctcttagttt
     2641 catagatgct cctagtttta tactttatca gaaattgtgt cgttatgcca agttagcaat
     2701 aaacacatga cggcagagaa ttatgaactt taaatcttgt gtctcagaaa tactacatca
     2761 tctgcactgg gtcgatatcg atttcgatat atacagacga agttggttcg atgctatgcc
     2821 ttagtgtctt tacgaatcgc tgcagatcgg aaaagcgagc cgccttaaag atgagttgat
     2881 atctatgatt gtcagcaacc acggctagag gacactcagc aggaccaaga atatctatgt
     2941 cttcaaatgg cgatatacac acaatgcgac gagcaagatc ggcatacttc gaggcttcgt
     3001 tgcgcgcacc tttcgctgac ttcgatcgga agacaaaacg acataagcgc cttgcgggtg
     3061 gaaattcaag ctgtcggcgt atttggagct cacgttcaag gaagcaaggc acatcgcctt
     3121 ccgcagctag ctgaataatt ggactatcgg gtttcttcgt ctggataaag acaattccat
     3181 cgggtacatg cctgccggct cttccagcca cctggacaat caaagagaat acgcgctctc
     3241 cagcacgaaa atcgggcatt gcaagccctt gatccgccat gaggatgcct acaacacgta
     3301 caccaggaaa attcagccct tttgcgacca tttgtgttcc cacaaggatg tcgatattac
     3361 cattgcgaaa agagagcaga gtattttcca gatagccttt tgtatttgtt gtatcagtat
     3421 cgagtcgcgc atggctcatt tccggaaatc cttcgttaag ctcttcttcg atgcgttctg
     3481 tcccatagct tgcccatgca agatcgagtg aaccacaaga tggacaggct ctaggtttaa
     3541 tttcatggta tccgcagtaa tggcatataa gacggttcga agccttgtgg taagtaagag
     3601 gcacggaaca atgcttgcag agcaattgct cgccgcaggt ttggcacatt agggtatgtg
     3661 caaaacctcg acgattcaaa aaaagaatcg attgacggcc tgcttgcttg gctgcatgca
     3721 atgcctcagc tagctcttca gagatgagac catgcctacc tcgcatgtct atgacatgta
     3781 tttctggaaa agaacctcca gccggccgat tcgaaagatt gaagcggcgc atctggcctt
     3841 cttcgcaagc ctgccatgcc tccggcgaag gggttgcgct gcccattaca aggcgtgccc
     3901 cggctaggtt cgctcggcgc attgcaactt gtctagcatg atagcggggc gttgtgccgc
     3961 ttttatagct gccatcgtgc tcctcatcta taatgattag tccgagattg gaaatgggag
     4021 caaatatggc gctgcgcact cctattacta tgcgcgcttc tttctttgct attctgcgcc
     4081 attcggctag cttgcgcgaa ggtgtcaagc gagaatgtat aatggcgcat tgctctccga
     4141 atcgggcgcg cgcgtcctgt tcgacttggc gcgtaagagc tatctcgggt actagatata
     4201 gaacacctaa gcccatggat aacgttgcgt cggcaagctg gagaaagacc tcagttttac
     4261 cggttcctgt ggggccgtag aggtagaaca tgcccctagg cttggcgaga atgccttcca
     4321 gcgcagccct ctgtccttca gttagtacaa gtggagttct gctcacttct atttcatcga
     4381 attgctcagc gatctctaat cgttcgcgcc gagccgatgg caacattgct gagagtgctt
     4441 caccaaggga acaatgatac atagaggcga cccatctgcc gagctctagc atttcgctat
     4501 cgaaaagcgg ctcaatatct attacccgat ttatagaacg aattttgctt ggttctagct
     4561 taattaagtc agttggtagt tccaactttt ccaaataggc tggcagttct ggcttggcca
     4621 attttgtcag ttgtgtcagc ttaggctttt ttgctgatga ttcatcttga gattcagctc
     4681 ccaaattcag ctcgttttcg gaaactggaa tatcgatgga ttcaacaaca taaccgacga
     4741 ctttcgccct tcttccaaga ggagcttcta cgcgcatacc tatttcggcc cgtgtcgagg
     4801 tatcgttgcg gtaccaaaag gtttcttcga gaggaattgg gtaggctatg cgcacgaatg
     4861 acataggcat tctctattcc tctagctttg aaatctgagt tgtaactgtc attttacctt
     4921 ctttgtggct ctgctttctg taagggctct gctttccatg agcgagcgga attgcttcaa
     4981 gtcctcccat gcagggcgtt tcagagctgt gtctctaagc agggctgagg ggtgaaaagt
     5041 cacacggaca ggaattcctt gaaaatcgaa gctccgaccc cgcagtctcg aaattggatc
     5101 agagagcctt aaaaggctgt gcgcagcaat acgaccaacg catagaataa agcctggctg
     5161 caatatatct atttgagctc gcaaatagcc tatgcaagca tcgcactcag aaggcgaagg
     5221 ttcacggttg ttgggcggtc ggcattttac tatgttagct atatagcagt tgttgtgccg
     5281 cgaaagatct atagcagcaa gcatcttgtc gagtagttgg cccgaagctc ccacgaaggg
     5341 caagccctga cggtcttctt ccattccagg tccttcacct atcaccatta cctttggatc
     5401 taagactccc tcgccaaaca cagtctgcga tcgggattgc caaaggccgc aagcagtaca
     5461 ctgactaacc tgcgtttcga gagcttcgag atgcgctttt cgatcccatg tcgatggtat
     5521 ggccgagcta ggcgcttcta gcgatatctt gtgctcgcac cttactccat aacggaaaaa
     5581 atcctcgatt aggaaaagag tctccaaaat ctgtcgggct tcagcgtctg tgtatgtgct
     5641 ttcattgtat gtgctttcat tctcattcaa agctttttcg ctcatagcta aatcaaatcc
     5701 tcgttcaagc cagaattcca tagacgacta tgtccgacta tgaccctagg gttctcaaaa
     5761 tctagactca ttatcgtcta tttgtatatt ctcattcgat atcgagctca ataaccgctg
     5821 tttccttaaa gcgaggacaa gcaatggcta ttctgatctt tccttttttt gctttgctgg
     5881 atatccaaaa cgcgctaact ccacttatca gagacctctg ggctggtccg aggaggcttg
     5941 ctggcccttt aatttcgata gagtaaggtt cgaatagaaa aggtacaata ttcccatatt
     6001 gatccagggc ttttacaaca atgcgagtgc ttggccactc ctcttctgta aggcctccaa
     6061 gccatagcgt atctgactct atagagagct tttgtgcgga gctatctgcg ccaaagctgc
     6121 gctcgaccac ttccttgcca tttaaaatgc ccactatgcg tatcttttca tccgatgctc
     6181 cccagttcat accataacga agaacaagtc cttcaatatc ttgaatactg agcttgtgcc
     6241 ttcgcataaa aagccccatt ctcagtttgt cccagagatc aaggctgctt tcgcccaaag
     6301 acattgcttt accagccagt tttcgaaaaa gacgaagatc gtttcggggc caaccctcag
     6361 cttcaagcaa tgctcctatc agatcatcga ttactatagg tggatgggga aggttcgcaa
     6421 aatgtatttt atcgggaaag aaacgagcaa caaaacctcc cgatctatag acatctatgg
     6481 catcgcagtt ggtaaagaca tggataggca aaagatgaga agcattcctc tcgcccttgg
     6541 caaatattga agctggctct aggacaatgc gctctgaggg cgagatttga ctgctataga
     6601 agagacctgc gtattttggt atccggaaag catcggctac tccatggtaa caaattctat
     6661 ctcctgaacc aaagtctttg tgagtatgat agtcaaaagc gcaccagccg atgcatcccg
     6721 caacgcgcga attgcccatg gcagtatcga gaatatgtgc atggcgcagc gcatgctcaa
     6781 caagacgttc ttcctgatcg aagcgttttg tgggaaacat gtgtcctgta tgttcggtta
     6841 tgagataggg tgcccgtttt cggtatttcg gaagcacttt cgcaggctcc gagatataca
     6901 tcgtcccttt gccatcatag atgaagtcgt tgaaggtata gacatcttcc aagagttcgc
     6961 tgcgggcttg acatcgaaca cctcctgtct ggcgatatgg atcgagcctg cgggcgagct
     7021 cgttggtcct ggcatagaac tcatgattgt ccttggattc gttgatacgt actccccata
     7081 aaacgatact cggatggttc cgatcgcgga ctatcatatc ttcaaggtcg tgcaaagcct
     7141 gttcctgcca ttccctgccc cctatatgct gccaacccgg aagttcttcg aaaaccaaaa
     7201 ggccaagctc atcgcaggca tcgagaaaat ggggcgattg aggataatga cttgtgcgca
     7261 cgattatgca ccccagttct tctttgagta tctcggcatc gcgcctttgg ccatctgggc
     7321 ccatggcata accgatatat ggatattcct ggtgccgatc tagcccacgc agaaatattc
     7381 ttcgctggtt taggtaaaat ccagaagatc caaattctgc ggtcctaaaa ccgatacgca
     7441 gctgctcata atcgagctct tggcccatct cgctaaagag cgctagttcg aggtcgtaga
     7501 gagcggggct gtctatatcc caaagtttgg gcctatctac actcatggag aaatttgctg
     7561 aaaacttgtt tttctcttcg attctcgtag aatccgagag aagtatgttt ccctcattta
     7621 cgagttctcc agacaagtaa aggcgggcaa gcaggctgca attattgatt gacccagatt
     7681 ctagggcttc gatttctgcg cttatatgta cgagccatgg tccatccagc gcgtctcctc
     7741 ttgggcgcgg gatcgcccac agcttgctca ttctagcacc taaatctgca cacaggctga
     7801 ctcctctata gatgccacca tatgcaagat aatcgactac ccctccaaag ggcggaatac
     7861 cactatcttc agcgctatcg acctctacga gaacccaaat tggtcttgct cgctcggcct
     7921 tcgaaccttg ctctttcgac tgctcttcca gccaggtatg cggtatgaat gtatcgatgc
     7981 gtatttcgaa aggcgtatat ggtcccttat ggctacaagc taatctgcca ttgacccaca
     8041 cacgacatga gacacttacg ccttcgaagc taagaaatat cgctggatat ctatttatgc
     8101 ttgttttttt atctaagccc tcagttatct tcaatatttc ttcttttttg agctctttag
     8161 catagcatcc ccagcgtaga aagcttcttt cgtcgaacgc attgaaggga gcttcgatca
     8221 tgtcatgcgg caaatgaatc tgtttccagt cttcggtttt attctttgca tcgtcggtct
     8281 gcgatatttc tttgcttatg attgtcttgt ttttagggga gtgggcagcc gatgggccaa
     8341 tccaatcatc gctcaaaaat gcatcttctg ctttcccggg cctgaaccac cagccggcat
     8401 tccatggatg cttcattctc atttaaccag cattacctga atgatttgca gtatcggaaa
     8461 gataatgcat actgcaatga ataccatttc gagggtcatc atgcgctgct ccgtggtatc
     8521 gctctttaat agttcataga tgttctgtag cgcatttaga cgtctttcta ccgaaaactt
     8581 ccactcgtct gtattgaaaa tttcgcagag cctatgatag atcgagccca aaaaataatc
     8641 tccgattatc tttgaggaat tatcgaggtt ttccaacgtg aaaagcgcat cgaaacgcaa
     8701 accctggatc tgggcgagct tgagcctaag ggctctctcg ctcgacctct tgcgtctgcc
     8761 agaaaaatac agtcgtcgga tgtcccgttc ggcttcgctc agccattcat cgagcaaaaa
     8821 atcgagttct cgcagctcta tgagtcgata gttagcatgt tctgccatca taagaacatc
     8881 gtcgtaatca gctgagggat ctatgatgag gcagcgatca aggtcgaaaa tcgcaatgtc
     8941 gtttcggcga taactaaagg atttgtctag aatctgttct atctggctaa tatgcaaggt
     9001 ccctgccgtc tctccagata tcaagctagc tgccatatcc ttatgagcat tgataaaagc
     9061 ttgaggatct cctccaggac attccagtag acaaaaagct atataagttt ctctgtcgct
     9121 ttcgctcagt tccttatatc cgactatagc gctacgcagg gcttctctga ccattctaaa
     9181 ggaagattca gcaaactgct cgatggaaga cgagccgctc acatcgcgaa ttggcatgtg
     9241 cgccttggca gggagctctt cgaacgtaga catagtgcaa taacgcacaa tcaccgttat
     9301 ggctccatcc tcatagatct ttgccattgc ggatatcttg ctgaattgtt tcgaatcgca
     9361 ttcctcggta ctgatggaaa gcacaagagg tttaggcaga gtcaaataag ctggggtatc
     9421 gcgccgtttg gctaaggcaa tgtctcggtg cgctggaact aaagaagcca ctttattgag
     9481 atctatcgat cttcccacat cgtaggcata gagatgaaca atctctaccg gtatctcgcc
     9541 acgctcgttc atcatttcac ctccttacgc gtcacctccg ctccgcttac cattaggacc
     9601 agcaccgacc cattctgtac agagaaaggt gcactgaacg cacccccggg atgcttcacc
     9661 gaataaatag aatttcttgt tccgtctgga gaaataacat caagtccaac tggtacgaca
     9721 tagggatact cgggcaagga atattcgaac acgcccgata catagccctt ccgcgcttca
     9781 ggtgtagcga ttgtgatctg cacagaatcg aattgcttga gatttgtatc gggctgcacc
     9841 gactgttcta caacggtacc gggtgtttcc ttttcatgcg cagaacgcat tttatagctg
     9901 aatactagcg gaattgtaga tgccttcgac gccatctccc tcatcgaaag gccaattaga
     9961 gatggcatcg taatattcct agattctggc cctttgctta cgacaagatc taagactgtc
    10021 ggaccagaaa tttccgtacc cggctttggt tcctgatcta gaattgtacc aggcgccgaa
    10081 ttatcgaaaa tataaagagg aggttctctg atgcttacga gggtctttgt tgtagcggaa
    10141 attccttgaa ggtagagctt gaggtcatcg atgttgcgac cggaataatc ctccactctg
    10201 tctagcacag caccgcggct cacgacgaga ttgatgcgtc gtccggcttt tacaatgctt
    10261 cctggtggag gtgactgctc aagcacgaga ttgcggtcga ggggattatc ggtgaaacgc
    10321 agcgtcaggc gcgggtatag ctcacgctgt tgaagtttga ctagagcgtc agcaaggtcc
    10381 atatgtctta catctggaac catcgttctt tcttctcctc taagcgcaag gaaaaacacc
    10441 aatactgcca caatcaccat gactagaaac atgactaggg cgaaccaaat aaaggtccgt
    10501 ttctgctctt tggtccactt ggagatatgt tcatgctgct cgcctgcgct atctgcgacc
    10561 gtctttgctg ctttggatac cttctgagga gctttagacg cagtttcctt tagtatcttc
    10621 gtaaaatctt tgatatcttt cttgtctttc ttgaccatgc tttaaaattc ctttacctgc
    10681 aactatcggc aactaaaata gttatgactg aaacggatct ctttacgtgt agctctctta
    10741 tcggagaacc gcgccaataa tgtctctcgc tccgttggaa aaatcctgaa acccgacagc
    10801 cttgcgagtc gctacctgta gcctttttat tgcaatgtag ccatctccgg cctttacgat
    10861 aattcccttc tgcacaactg ctgcaattat agtcccagga gagtgatttg tcatatatcc
    10921 agcattgctc attgaatcaa aatcatcgaa aggctctact tcaagaatgg aaaggcgcaa
    10981 atttctaaga aaagtgaatg agcctggcca aggatcgaag gcacgtatgc gggcatcgat
    11041 ttcgcgcgat ggcctatgcc aatcgatatg gccatcttct ttagttattt tcctgcaata
    11101 ggtaggttct cctatctgag gcctaggctt tgcaattcct ctttctatct catccaaaac
    11161 ttcgacaacc attgggcctg caagctgcgc acagagttcg gaaagcgaag cgcttgtctc
    11221 tcggccttta agaggaatgc gatgaacatg caagagctct ccagtgtcca tttcgggagc
    11281 tattgtttgt atcgatattc ctgtctcctt atccatgaac attatggcat ggggtatggg
    11341 tgagctgccc cgccatcttg gcaaaaggct tggatgtaca tttatgccgc cttttgggaa
    11401 aagctccata aatctaggtc caaaaatccg tccatacgca aaagagacaa gtatatcagg
    11461 caaaacggcc gaaactgcgg ctcgtgcttc agacttgagt gtctcaaaag caagaatcgg
    11521 cacgctctct ccccataggc gtaatgctgc ctgtgctatg ggagtactgt tcatcttaag
    11581 gcctcggccg gctttggtta ctggattcgt caatactccg acaacctcat gcgaagaatt
    11641 gtgcaaactc tctagggtgg gaaccgcgat atctggattt cctgcaaaca aaatgcgcac
    11701 cagcgactcc tagccgtttt ttacattttc agcatacggc catagtgtgt aagggcccgt
    11761 tttcttttcg aaggtgagac acggtctaca aataaaatgc catctagatg gtcatattca
    11821 tgctggataa ctctagccag caacccatct gcgctgattc tgaaaggttt tccatgcatg
    11881 tcaacggctt ctatatctac ggcaagagga cgaactacgg taaaatatag ccctggaaac
    11941 gaaagacagc cttcttctaa ttctgtggtc tcctgactgc tagcgacaat gtggggattg
    12001 atgaaaacgc gaagaggttc gtcatcgatg cctacgataa agagccgctt ggaagtcccc
    12061 acctgaggag cagcgagccc aattccatga tcgcgctgca tggcttcatg catttcctcg
    12121 atgagccgtt gtaagctttg gtcgaattcg gtaatctcat ttgctttctc ggtgagaatc
    12181 gattcaccat aggtcaatat attgagcata tcaatttcct atattaagca tatttgtgtt
    12241 tcagatatat agattaacgt aaaagatgac ttcttggcaa tgttccatgt atttgttttt
    12301 attctaacga tggaatctgc accttatctc cgactttgat accagcgcgt tcgaaccagc
    12361 ctttaggtac ttctagggcg tagcgtacgc ttctaatcga gggacgagat tcctcagaat
    12421 aaggcacaag atcgataatc tggacaatag ttccatcaga gagaatatag gccagcgaaa
    12481 gtggcaactt ggtgttcttc atccagaaag agagcttctg atcgctctcg aaaacgaaga
    12541 gcataccttc gccgtctttc aatgagctgc gatacataag gccgcggttt ctttcgcctt
    12601 ccgtaagtgc aacttcggcc ttgatttttg ttgttccaat tacgagatcg accgtcttga
    12661 gtttagggtt tggcttatct atgccttggc cggagtcaca cgctgtaaaa agaatgagag
    12721 ctgccataaa tggaataatt attagacttt tagataagta tcgggttggc gtatcttttc
    12781 tcataatcta tcttttcctt tatctatatt aagcgcttct attatattaa gcgcttctat
    12841 gataaccaac tttgaaaaga aaaagccgct tgaacaaagc ggcttttctg cgagcatcat
    12901 acttcctctc gaagatcaat atctgccttc ttgtgatcta cgagtttttt tctggagctt
    12961 ccgttctagc gctttttttt ctctattgcg aatcgcagat ggcttttcgt aatattcgcg
    13021 ccgcttccat tcacgtatga taccttcttt ttcaaccatt ctcttaaagc gctttaacgc
    13081 cttttcgagt ggctcattat cttcgacaat gatttgagaa ataaatctca cctcccttct
    13141 atatgcggag tgcattgtaa ctgaaggaaa tattttgtca agctctccgt ttatatatgg
    13201 cagctctttt gcaaatctat tttgaaggct atacttggat agaggagttc ttagatgctg
    13261 ttttcacttg gtaatctgat aacactggcc gttgtcattg cgatgtttgt agcctatcac
    13321 ctgttgaccg cggataatcg ttctctcgaa aaactgaaac gcctcggaga caagctcaag
    13381 gatgagcttg gttcctatgt cgaatcaaaa gccgaagaag taaagcacta tggaatcgat
    13441 ctcgatgtgc aacagaaggc agccaaagta gtgctggaaa aaatccagga ggctcaggca
    13501 actctagatg agaaatcgga tacgttaacc gagatggcgg agcgcgttaa gaaatatgac
    13561 aatgttttat cccagcttat ggaaatgaca aagcgtgtcg atcagaatat cgcgcacctt
    13621 gccgaaaaga atgactttat acaagcaatt tcgcgccgcc ttgaagatgc agataaagaa
    13681 attgaaatta tcgaaaaaga aatgcccaaa ctgaaggaac agtttgctca tgatgctcag
    13741 caaatattgg atggattccg cgacgatatt cttgatcaac tccatgagca gctaacagga
    13801 atcgtcaacc tgtttgaaaa atctagaagc gatgcagagt cagcaatagt ggaagctact
    13861 atgcttaaag aaaagatcga tgagattgct acagccgccc tttccgcagc tgcatctcgt
    13921 gcgcacagca ttgaggattc tgcctttgcc acccttcgtg cccacattgc caacaatttc
    13981 gacgaaatct cacgccagac cgatgctcgc atcgaagggc ttagcaaaag cattggtgaa
    14041 ttagcaaatg ccatagaggc caaagtagcc gccctcagca cagataccgc atcccagctt
    14101 gccgagtccg atcgtagact ctctgaggct agagctgcct tgaacgcaat gaccaacgat
    14161 atggcgcgca ttcaaaaaga atcaacggaa atcaccattg cgctttccaa agaattcaaa
    14221 gccgaacgaa attcgctcga agaacaattt gagcaattcg gtcaggcttt cgagattcat
    14281 cgcgcatcct tcgaaaaaga attccttggc gagatatcgg ctctacgcga atccctagag
    14341 gaagcaaagg ctcaaagcga agaactcaaa agcgcagccc agggcaatat tgccatggct
    14401 ctcgatgaat tagaaaagca ggtaacccaa gaactcgaat ctcgaaggtc agccttggat
    14461 gagcaaatag atgaatggct cacttcgatg gatgccaaga tccgcaaggt ctctcaggac
    14521 gctctggatc agcgcaaggc agaggaagct cgcctagctc aggaagtcaa cgcggaattg
    14581 aagcgtctca aggatagcct ctatactcaa accaaaaaaa tcgaacgcga tatagaagcc
    14641 tttaaaagtg tctgaaaatc aagcaaatat tgcgtcgagc gccaattcta ccatggcacg
    14701 gaaagagctc tggcgttcct cagaactagt aatctccccc gtaacaagat cgtccgagac
    14761 ggtaagaacc gagagtgctt cgcagccaaa cttagcagcc agtgtataga gctctgcggt
    14821 ttccatctct acggcgagaa cgccaaatct tgcccagagt ttccattgct cgggatcctc
    14881 ggtatagaac atatcggaac tcagcactga acccgcaaag actcgcagtt tgcgagctgt
    14941 tgcggcatcc caagcagcct ttagcaggtt gaatgaagct gtgggagcat aatccatacc
    15001 tttgaatcgt agatggttag ctgctgaatc ggtacaggcg ctcatggcta gaacaatatc
    15061 gcgaaccttt agatattcga gcatgcttcc acaggttccg atgcgaataa gcttctttac
    15121 atcatattca cggatcaact cattgacata tatggaaaga ctggggattc ccattcctgt
    15181 gcccattacc gaaacaagct ttcctctata ggtacccgta tagccaagca tgttgcgtac
    15241 ttcattgaag cagaaagcat tttcgagata attctcagca acaaatttag ccctgagagg
    15301 gtctccaggc aaaagaattc tatcggcgat ttggcctttc tgcgccgcga tatgaatgct
    15361 catcttgcag tccttatcaa tttaaaagat ctaagctaat tagcctattt attcaatcat
    15421 aaaggctaac agaactcaat cccttatcaa agactgctac aatcatggca ttccccccag
    15481 ctttgcttct tttattattt atggccctat ttcctgtttt acttcagact ttggttccca
    15541 ccttactttc ccaaaccttt gcgcgagatg ccaaaagcat agataggccg catatagctc
    15601 gactcgccga gcattaaatc gagcatgacc gcattgataa ggccatgatc gccagctaga
    15661 gcctctgcca atccggaagc atacacttca tttaatggct ctggaccttc cgtagcaaaa
    15721 cgataatctc ctttgtcttg ataccataaa gttatctgga atccttcaat attctcggta
    15781 tcctctggtt ttgtctggtt atacacggcc tttcctttct caggataagc ccaaaagcct
    15841 ttttctagga ttgcatcggc ttttcgtatt tctgatggaa gccgctcttc ggatggatcg
    15901 ctggaataga tttccttata ggttccatca ggcgaagaaa atacaagtga cttatccaga
    15961 gtcacaacaa cattgtgtgc tcccgagatt tcgattctat ttccttttgt ttttattgta
    16021 taagaccagg gttttttttc cgcgctcgaa gctgctagcg atatcgtatc gccagaaaca
    16081 ctcgcattgt attgtgcaaa ttccttttct cctcctgctg tgcgctgata tgaagtacca
    16141 cccactatct gtcctgatcc gtcggttagt agacgaaata tatatgtatc gaagccataa
    16201 gcttccatat atcctgggcc tgtgtgctta tagactatga ggatattctc attttgcttc
    16261 tttatttctg gcaggttcgc accagcttca ggatgaggtc ctggtgtacc gatagccttc
    16321 tgagtgacgt cggtcttggt gcttgtcttg gaagctccat cttctagagg cttctctaca
    16381 actttagttt gtttgcatga tataaccata aagaccatag ccatcatcga gatcagcaac
    16441 aaagcacttg aaaaacgaaa actccttttc atacaatgcc tcccctaagc atacataact
    16501 atactacatt acttcagctc aaataagagc ctaatctaat ttgttacatt gtaaattatt
    16561 aaaagccacc ttaaaagccg ccatcttctc tgccatatac cccttgagcc gatagatggc
    16621 ggcaagcata cattttttat tcgatattca tatatcgatt ttcttcactc tacatatctc
    16681 ctcgattccc aaacaattta tgcggtttct ttacaggtgg ccttaccaat attttttgca
    16741 cagcttttgg attctcttct ctatcttgtt ttagaactcc agccactcta gacaaatcgt
    16801 cctgcttaac tatgagacat ccataggatt tagacgcatc gaaaggattc gtattagagt
    16861 gcacggcata tccagtgtct ttatacacgc cgctagcctt ataatttccc ttgccatcat
    16921 tgacataagt gtcgactttt ccaaccgcat ttgtagagat catataaggc ccatatttgt
    16981 cttctctagg cgttacagcc gtgatcgacc aatacccttc tggaaactgc gaaggcttac
    17041 ttgttaccga cccagatgat gcgagtttac gcccagcagc atctgctggc ataaaaggcg
    17101 ctttttgtgt gtctacgact cgattatgaa catcaatctc aaacacctct tttggaggga
    17161 ttcggcccaa tcttgcctca tactcattat cgattacatt tactttgagc tttgttcgat
    17221 cctgattcag cgacatacgc acatcccctc ccgttacagc ggcactctca tcatggtcaa
    17281 ggggttcacc aagattgaga aaccgtacaa cagggacgaa cgcttcgccc tccactgcga
    17341 aggcgaaggg ctgcaatact gctcctaaaa gcaggcatgc agcaatgaag attgcttttt
    17401 ttgtgcgcat ttagcacctc ctcactgtat agacgctgag aaggtatgaa tatgcttcaa
    17461 agttttagat tttttggaaa acctatctag ctctatcgct agactctaaa ggatggaaat
    17521 ttatcaatgt ccattgagca aaagctatgg agttccgctt tatgatttct atctcatatt
    17581 tagaaataca acagttggat attggaagaa gaggcaacca atcgtgaact ccgagggtcg
    17641 atcttataag ccattgcaag agaccgccgt gtgtaatgca gactattcga gaagctcccc
    17701 tggcggcaat ctcgcgaata tgctgccagg ccataatcgc ccgagcgtaa atcgattgcg
    17761 aagattcggc ttcgggaatt gcatcccagc ttctcttata gaaatcatcc catatttttg
    17821 gatgccgctg agccgctatt tcggggttaa ggcctgaaaa tataccagta tctacctcta
    17881 tgagattatc gtccaactca atatggtcta tatttattgc tgaagcgaca atttcggctg
    17941 atctcttagc ccgcagcatt gggcttgcta agatatagtc aggtttgaat tggcgcaacc
    18001 attctccagc ttgcctagct tctttttcgc cttgcacaga gagaggaaaa tcatatcgcc
    18061 cctgaaaggt attgcgggca ttgccttcgc tctgtccatg gcgaagaata aaaacctcta
    18121 gatcattatc aagagatgca aaaaaagcgc catcggctct tatatcttcc aattctttga
    18181 taaggtgcgc atctggaata tagcttggct cgaatcgcgg atacatctca tcctctaggt
    18241 ggcacaagaa ttgatggttc caatcatgga accttataaa atctcaagca gcgataaatg
    18301 tctcacttcc ctggttaaag tatttattta cgataaacat tgcgataccc gtcacaaaaa
    18361 gaagaatggt cgacattgcg ctcgccgtgc caaaatatcc atccgccacc gaaagataga
    18421 tctttatagg cattgtcatt gtcttcccta catataggag aatcgaagct gatagctcat
    18481 tcatagaagt aacccaactc attattgcgc cggagattat tcctccctgc atgagcggta
    18541 gggtgatagt tctaaatgtc cgccctggcg gagagcccag attgatacca gcttcctcta
    18601 gagctggatc aatctgcttt agaatagagg cggcggagcg aaccgagtag ggcaaacgcc
    18661 gaataaaata agttagaata attatggtcg ccgtgccagc cagtattatc ggtggcttgt
    18721 tgaaagcgac tataaatccg attccaagca cagtaccagg aaccatatat ggaatcatga
    18781 gcaatggatc gagtattctg acggctgcat tcctttttct agcaattacg aatcccaaaa
    18841 gcgtcccaac aaaggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacag
    18961 gcctattcac cagcatgctg gcatatttgc gcctcgaaga aacccactgc tgtagcacaa
    19021 tcacaaccga cgcacagagc atcaggatga tcgataccgt agaagccatt cctggagttt
    19081 ggctgatttc gctcgtatag agattgtagg caagcgtcgg cagcacaaga taatttccgc
    19141 ctattatcat tggagttccg aaattggaaa gcgacatcat gaatacaatg agagctccac
    19201 ctgtcaccga tggcagaact agaggcagtg tcacgctgaa aaatgtcttt atcgaacgag
    19261 ctcctaggtt ttcggaagcc tcttcgagcg agcgatctat ggtcatgaga gcgccggctg
    19321 tcataagcga tatgaaagga taatactgta gcgtgtcagc gagcactatt ccgagcggac
    19381 catagatagg cggcagattg atgcctatag actggaacaa aagtcgcagg aatccatttc
    19441 gacccaacat ggtaatccag gaatatgcac caataaaagt tggcgaaagc aaagccagca
    19501 ccgatagcgt attcaggagg ttggtgcctt ttatgcgata cctagtggta aaaaaagcaa
    19561 gaggaatccc caagaccatc gaaaaaaatg tggtcaaaat agcgatcacc atcgagtgcc
    19621 agaaggcacg caggtaatag actttggaga aaaaagtctt ccagttcgac agagaaaaag
    19681 tctgtgtttt cttatccaga aagctatagc gaaaaatgtc gaatagtgga tagatgagaa
    19741 acacaatgac aatgacaaaa ccaaagaaaa tgacaaacga ccagatatcg gctcggctat
    19801 gccgaataga aggagtctcg gctttctggc tcatttctgg gctcctctgc ctattctgag
    19861 gccagtcttt ggatcaaaaa ggagcatgga atctgacggt acggcaaaaa aggcatgttc
    19921 gcccttttga gggatatcgg attcgcgagg cctatgccta tcgatcgtta tctcctgacc
    19981 ttccacctcg agttcgaagc gagctaccga tcccatgaag cttacggttt ttagaattcc
    20041 catgaccttg ttctggcttg cctcggtcgc agcctcgatt acatcttctg gtctgaaggc
    20101 gactatcacc tctgaaagac cttcagtacc ttcagaaggg acaaggagag ataaatttcc
    20161 cagaactgca ctgcgaagcc ccccctctgc cccttcaagc aggtgcatgg tgagcatatt
    20221 tatctttcca acgaaatatg caacgaaagt gttctttggg tctgcataga tctcccaggg
    20281 tttgcctatc tgttgaatga ctccctgctc catgacggca atgcgatccg agattacgag
    20341 tgcttcctcc tgatcatgag tgacatatat cgatgttatg ccaagcgatt tctgcacagc
    20401 acggatttct tcgcgcattt ctatgcgaag tttggcatct agattcgaga gaggctcatc
    20461 gaagagaaga accctgggct gaattgccat tgcgcgcgca agacctacgc gctgctgctg
    20521 tccgccagag agcttgtctg gggtgcgctt ttcgtagcct tcgagatgaa cgctcttgag
    20581 cgctgctgaa actttacgtg agatttcatc cttcggcaat ttccggacac gaagcccata
    20641 ggctacattg tcgaacaccg tcatatgtgg aaatacagcg taattctgga acaccatggc
    20701 ggtattcctc tgataggcag gcactttatt tatgagttcc ccatcaatgt agatatcgcc
    20761 ctgatcttgc ttgtagaaac cggcaatggt ccgcaaaagt gtcgtcttgc cacagccaga
    20821 agggccaagc agcgtaaaaa actcgccttt ttcaatctct agattgatat cggagattgc
    20881 tcttaattcc ccaaagcgct tttccaaatc cacaattcgt atcgaggtca tgcttacctc
    20941 tcgctataaa agttaagaaa attgtatcta actatgcgac tctcttcaat gttgtctgca
    21001 tctctagcta tatgatgatt taaagcaaat agcaaaaaag ccgccagctc ttctgctggc
    21061 ggccttcgtt tccgcaaatc caagcaaatt tagcaaaaag gattgcggct ttgcatcatc
    21121 tcacagacca agctccatcg cgatatccat gaattttgcg atgaaggctt ccttattctt
    21181 tgctgcccat gcaaaatcat agggtattgt cttgatagtc gaaagcggag gcaagccttt
    21241 gggatccttg caatcgatcc gaacagggcg tctagccatc gcatcgacaa gatactcttg
    21301 gattggcttg gaaagacacc aatcgataaa tctcttggca ttctcgaggt ttggagctcc
    21361 cttgagcaag gcaatgccat ctggggccgc aacagtgcca tctgtaggat agacaatagc
    21421 tacagggcca ccaccagcaa agtatctaaa cgcattgtct tcaagagtta ttccgactgc
    21481 ttgctcgcca tcgtttacga atttgggcac agctcccgac gaggctgaga tgaccgtatt
    21541 cttcatgata cccttataga tgtcccatcc cttgtcgcca tagatagcaa tgacattgca
    21601 gagctgcatg taggatgaac cggatttatc ggccctggcc gaacagatgt ttttcttgaa
    21661 tctcgcatct gtgagatcgg tccaggtctt gggatacttg tccggcgtga gcatcttggt
    21721 gttgactacg aatacattca tgataccggt gtaagggagc cagttcgttc caactttgta
    21781 tacggggttg atcttatccc aatccttcgg tgtatatggg gcaagaatag agttctctgc
    21841 ttcgagctgc tcgccaccta tactccagat gacatccgcc tgtggccttg atgcctcggc
    21901 cttggctcga ttgatcacat cgcctgaagc catcttgaca tattcgacag taataccggt
    21961 ttccttttcg aacatgggca ctatggcatt gataatgctc tcctcgtgcg ccgtgtagac
    22021 cacaactttg ccacccgctg cccatgcgga taccgcaaga agcagacaaa gaatcgtgac
    22081 cacaaatcgt ttcatagaac ctcctcttgt acctgcggct gccattctag acacctgtcg
    22141 cgccgcaatg tgcattttgt tcgctttatc tattgtaaca tattttgcaa ttctagtgag
    22201 agattatgta tgcgaaaatc gtaacaaaaa gcatccattc cgagatcctg agcagcctta
    22261 cagtaatcac cgatatcgtc gatatataga caatcttttg gatctaggcg tatatattcc
    22321 aaaagcttca tccaaatacg aggatcaggc ttcatggctc ctattttgta ggaaagaata
    22381 tagagttcag gataggtgcc cagcacttca ttgtcgaaga tcaccgaggc atgaagaaaa
    22441 gaggtattgg ataagacgaa ggttctggca cccaggcttg ccgcggtctc aagagcacgt
    22501 tcaccatctg gattgggcac gattatggat tgatagtctc ttacaaattc ttcgtagcta
    22561 tagctgggtt cgagctctac aaggctctgc acgcgtctaa agtattcgcg cgagtcgatt
    22621 ttgcctgtct cgaactcaat ttcgattgga gtaccccaga gggtgcgtag aagatcctga
    22681 gcattctgtg cttttgcatg aatggtagct ttttgaataa aattctctcg ttcaacagca
    22741 catagcacat tgccgaagtc gaaaacaaca gctttataca tggtaagtcc tttaatttaa
    22801 taatttataa agagatatta taaataatcc acttacatgg ctattccaaa aatcgtgctc
    22861 aaagcgaaga ttttgccttt accctttctc gagcctctag cgcaggcttg aaattcgatt
    22921 ttagggcaag agaggcattg aaagcctcgt aggctcgtcg gtactggcct agaacctctt
    22981 tgacaagtcc gaggcggtac caccagtaat acatattctt ctcaagcgct acagccgtcg
    23041 aaaatgctat gtccgcatgc tcatatttct ttatcctaaa ataaatttct ccaacataat
    23101 aataagcact ggagcgtcgg tcgtcgcttt cgggggcaat tgcaaaatag cggctgaagg
    23161 ccgaaagagc ttcctcattc ttattgagga agaagagcgc ttcggccatt gtctcaagaa
    23221 tacgatgatc ctgtcttgta ttcaacacag attgggcata gacaaccgta tctacatatc
    23281 gatgcagacg aaaaagattc caagttttga gtgtctgtaa gtcaggattg gaggggtctg
    23341 cagccaacaa gcgatctgtc attgcaagag aacgctgaaa ggcgagctgg ctttcggctt
    23401 ggcgagtctc tgccatttca aggcggcggc catcccaaaa aagagataaa gccgcattgt
    23461 agctatcctg tgcccccaga ggcagaattg caatggctat ccatccacaa acaagaagaa
    23521 tcaaataata ccttgccaga aaaggatgcc ttttgccctg tgacactcca ctcgcctcct
    23581 ctcctttatt gaagaatttc gtttatagat tgggcagcac gcctgccctc gcccatagct
    23641 agtatcacgg tagcggaacc cagaacgata tctccgcctg catagacgcg ctcgatgctg
    23701 gtcttctgtc gttcatcgac tatgatatgg ccatgacgat ctacctggag ttcgggagtt
    23761 gttttcgaga tcaaaggatt ggattcgttg ccaatggcta cgatcaccgt atcaaaggga
    23821 attatctctt cgcttccatg gatgggaacc ggctttcttc tgcccgattc atcgggctct
    23881 cctagctcat aagagagcac ttcgagggcg gcgacccttc ccttgtcgtc tccaataacg
    23941 cgcgttgggc tctttagaaa atcaaagatt attccctctt ccacagcatg ttctacttcc
    24001 tcggcacgtg caggcatctc agaccgagtg cgtcgatata gaacccgtac ttcctccgct
    24061 cctagcctga gtgccattcg ggctgagtcc atggccacat tgcctccacc caagacagcg
    24121 acacgcctag aaggatagat tggggtaccg gccttgcctt tcatgtaggc cttaagaaga
    24181 ttagttctgg taaggtattc attagcgctg aaaaccccta ctaggttttc tccggggata
    24241 tccatgaatt tgggtaagcc agcacctgtg cctatgaata ttgcttcaaa gccatcttcc
    24301 ttaagtagag aaagaagcgg acgggttctt ccaacgagga aattgagctc aaaatcgact
    24361 cccatcgatt gcaggacctc tgcttcagcc tgcacgagga ccttaggaag tctgaactcc
    24421 ggaattccat aaaccattac acctcctggt ttatggaagg cctcgaatat agcgacgctg
    24481 tggccttctc tgcgcacatc gacagcacag gtaaggcccg caggacctgc gccaacaatt
    24541 gctacctttc taccagtcct ctgagaaaca tgaggtattt ccacggcgcc atggccgcgt
    24601 tcataatctg cggcaaaacg ctcgagcctg cctatttgaa tagctttttc aacactatga
    24661 agagctttgc caaccgtaca tggcgcttgg cattgcgtct cctgagggca aacgcgtcca
    24721 cagatcgaag gaagtaaatt ggttcttttt attattctag cagcacctag gaagtctcct
    24781 tccttaatct tgccgatgaa ccctggaata tcgactcgca ctggacagcc tgcaatgcaa
    24841 ggagcattct tgcactgcag acagcgctct gcttctaaca tagcttgtgc ggaagtgtat
    24901 cctagggcga cctcattcat gttgctgcga cgctcgattg gatcttgggt aggcatttcc
    24961 tgaggaggta tctgcattct catctttggc gtaattggtc cagagcgtag ccgagaaagt
    25021 tcaagttcgg catcttccat tgtgaattct tccttatggc tcattttgct gactccttga
    25081 gagtagacgc tgtaagttct tctgcttcga gcttctcgag ctgcaactcc agatgacagc
    25141 gatcatgagc ttctttttct atgtctctat atgtgccaag gcgaatcatc attccatccc
    25201 aatcaacaag atgagcatcg aattcaggtc catctacaca tacgaatttg gtctttccgc
    25261 caatagatac acggcaacct ccacacatgc ctgtaccatc gagcataatg gtgttgagag
    25321 aggccagcgt tttgatgcca taaggcctgg ttgtctctgc acagaatttc atcatgatgg
    25381 gtgggcctat ggcgactacc aaatccggtg gtgggtctct cctacagagc tcatcgagcg
    25441 gttctgtgac caatgccttt cttccgaagg aaccatcgtc cgtgaccaca atatgttcat
    25501 ctgcaattgc tgcaagctcg ttctgcatta taagaagatc ctttgttcga gcccccgaga
    25561 tgaccgtaac cctattcccc gcttgcttta gagcttttgc aattgggtat aacggagcta
    25621 ggcctattcc accgcccaca cagactgtcc agccgtagag ttcgatatgc gttggatttc
    25681 ccaaagggcc aagcaggatt accttgtctc ctggattctt gagagcaagc ttatgcgtgc
    25741 tggcgccaac ggtctgaaag atcaaagtca ctgttcctcg atctgggtct gcatccgcta
    25801 tagtaagtgg aattcgttcc gaatactcat catcgcacat cacaatgaca aactgccctg
    25861 gcttgcgttc gcgagctaca aggggcgcct caacttcgat tcggaaaacc tcttccgaaa
    25921 gctgtacttt tgaaactaca ttatgcatgc tttgcatttt taactccgcg gatattttag
    25981 cggggattag tgatttttgt ccataaacca aaccattccc tttgtgctta ttccttttgt
    26041 aatctccgct ttttgcagtt atgcttcgaa tatgcctata caatactcca acttgagaag
    26101 agaatagtac aatagaaaac cgattaaaag aagaaggagt accgatgtcc ctaccaatcg
    26161 tatctcaaaa gctcgaagat gaagcccttt tcaaacctct cgattttctg gcatacctga
    26221 aaaagaccgg aattgcattg tcgcataggc cggcgcctga cgccattatt ttaagttacc
    26281 agaagagcct ttccgatttt gttcaggcta catatccttg cgagcgagcc aaaggctatt
    26341 ttggccactt cgttcattac ctagatttat caaaggcaga tataccagat aaggattcgc
    26401 tgataagcaa taagatcatc gctatctctt cagacttcgg tgtgggagct ccggctgccg
    26461 ccgtaatgct agaggaatta atcgcatggg gagtgaggga attcatatcg attggattcg
    26521 caggatcgct ccgcgaagat ctaccaccag gctcgcttgt ggtctgcgat aacgcttttc
    26581 gcgatgaagg cacttcctat cactatatcg agactactgc accggcgact cccgatccca
    26641 ggttaacatc gaagctagag agttgccttg cttctacagg actttcttac acgaagggac
    26701 caacttggac cacagacgcc atttatcgcg agactccatc agaagttcag tacttccgcg
    26761 aacagggtgc ccttgtggcg gatatggaag cagctgcgct ctttgcagta gcgaaatacc
    26821 gcagcgtccc tatagcatcc tgtttttcgg tatccgatac gcttgcttat ctggaatgga
    26881 ggcctgagtt tcatgccgag ccaacaatgc aggggctcga gatgttgttc tcatgtgccg
    26941 taagggcgct gaaatagctc agcaatagca ttctaatatc atcaagcgat gtaaaaacca
    27001 ctttcaagta tgtactctgc tctctgccgg ccatgtaccg gatacagggg acataggcag
    27061 aacgacactc ttctctccca ggatcgtgcc tggattcaat accgaattgc agccaatctc
    27121 gcaataatca cctatgagcg ccccgaattt tgcaagtcct gtttcgatct tttcctgaga
    27181 gtctagagat tttaccctaa caggctttcc atcgagtcta aagttcgaaa gaattactcc
    27241 cgctccaatg tgggcatgat gccccaggac agaatccccc acatagttga agtgtggcgc
    27301 ttgcgccctt tccaatagaa tacaatgctt gaattcgctc gcgtgcccca atacaactcc
    27361 tgaacccgcg agcaatcctc ctcgaatgta acagctacta cgaatttcgc aaccttttcc
    27421 gatccatgcg ggtccgcgga taaaagctcc ttcttctatg atgcagcctt cgtcgatgaa
    27481 aacatcagct tcaatgtgaa caccttttgg aacagaagat cggattcttt ctcccgatac
    27541 cgcatcaagt ttagatctga gataggaatc gagtagcctg agcacatccc atggcacatt
    27601 gacttgcgca aacagatcct caaattcggc acattctcta tattcattgg gttcaaagcg
    27661 ccatagtcta gggaaggact cgattttctg gttattgaaa agaggagatg gatagactcc
    27721 ttctgctacg agcctagaga tccgttgcct tacttcttgc agatcttctg ggttggtaag
    27781 gccaaaactc aactcgcttg cagcgagcat gcgcactcgt gctccttttt tctcgaccag
    27841 gctctggatc aaatcgggca acaggaattc ctgcgtagga gattcgatat atcgatctag
    27901 aaattcgagc cagccttgcc atgcataatc gaaaatagag gaattcaagc cccaaaagtt
    27961 catggaaaca taagtatcag gacttaattc aacgatactc tcgccttgtc tagaataaaa
    28021 acagtttcct tcgcgccata ccttcttgtg ttcgattatc ttttcaagat atccatcgtc
    28081 gcgtaaggtg catattcccc gtgacacagt tccagaagct ggaactacat cctccaattt
    28141 atagcctgcg aagcataatt cttcgggatt atgaaataga tgggaaccta ctgcctcgaa
    28201 tgcacggcgg ccatagtaat cgtcggcatt gataattgca aaagtctcgc cttggacgaa
    28261 aaactctcta gtgcagagaa gcgcatgagc cgttccccag ggtttgactc gcttactcat
    28321 caaaaggcgc ttactctctc caaggccgcc ttctcttgca ttttcctcat tctctccata
    28381 gatctctata agggctttat ccaatagaga gtctaatgtc tgataagcaa gtgaatagcg
    28441 aatgtccgaa ggaaggcgcg aaaggacatt cgacctgaaa tcagcctcga tctctggccg
    28501 aatcaggaat ataacctcgt tgaagcctgc tcttcgagca tcataaatcg aatactcgag
    28561 caaggtctct ccattaggac caaccccagt aacctgcttt actccaccaa atctggaacc
    28621 gataccggct gcaagaacca atagtttcat ggctaaatga tgcgcattct aatggctctg
    28681 gtcaagctag cgcttttcat cttctgccga tcagcctttg gtattttata tagtttttta
    28741 tatatataga gctaatactt cttagctcaa tcccacaaag gatccataat gccaaatcgc
    28801 tattgaaata tccatagaat ggttgtagga tatagcgggt caagtttcga gaccgtattc
    28861 taatatctta aatcttagag gaataacaca atgactaata ccaagaacat ggtaatgatc
    28921 gacggaaatg ccgctgcggc ccatgtcgca cacgcatgtt cggatgtcat cgctatctac
    28981 ccgattacac cttcttcgcc tatgggagag ttagcggatg aattctcttc aatgggcaga
    29041 aataatatat ggggtactgt tccacaggta gtcgaaatgc agtcggaggc cggtgcggct
    29101 ggcactgtgc atggtgcact tacaacaggt gcgctatcca cgacatttac cgcttctcaa
    29161 ggtcttttgc taatgattcc gaatatgttc aaaatcgcag gcgaagcaat acctactgta
    29221 tttcacattg ccgcacgtgc gattgccgca catgcactct cgatctttgg agatcatcaa
    29281 gatgtgatgg ctacccgtca aaccggctgg gctcttcttg cttccaacaa tgtccaggag
    29341 gttatggatc tcgctctcat tgctcatgct gctacactca aggcgcgcgt tccgtttctg
    29401 catttcttcg atggtttccg cacttctcat gaggtttcca aaatcgagga actctcatac
    29461 gacacaatac gagcaatgat tcccgaagaa aaggtgcgtg agcatcgtgc ccgtggacta
    29521 agccccgagc atcctgttat tcgcgggaca agccaaaatc ccgatgtcta ctttcaagga
    29581 cgagagacaa taaacaagct atatgaggcg actcctgcta ttgtcgaaga gacgatgaaa
    29641 cagtttgccg aacttactgg ccgcgcgtac aagctcttcg actatgtagg cgctaaagac
    29701 gcagagcgcg taataatcct tatgggctcg ggcgccgaca ctgccgaaga gaccgccgaa
    29761 caccttgtaa aacaaggcga aaaagtcggt gtgatcaaag ttcacctcta caggcccttc
    29821 tctgtcgagc atttcatcaa gaccatccca gcaacggtca agtcaatcgc ggttctcgat
    29881 cgcacaaaag aatcgggctc tattggcgag cccctctatg aagatgtacg aacttcaatt
    29941 ggggaagcaa tgggcaatca ttctgcacct ttcaacaact ggcctacagt ggtgggcggg
    30001 cgatatgggc tcggatccta tgaatttaca ccggccatgg tcaaggcagt attcgacaat
    30061 ctgaagcttg agcatccaaa gaatcacttc acgatcggca taaacgatga tgtgagcttt
    30121 acgagcttgc cctatgacga ggaattccac ttgccaggcg atggtattgt tcagtgtctg
    30181 ttctatgggc ttggatcgga tggaactgta ggtgcaaaca agaactccat caagataatc
    30241 ggcgatgaaa ccgacaactg cgctcaaggt tattttgtct acgattccag gaaagctggc
    30301 acctatacaa taagccacct tcgctttggt cccaagcaga taaataagcc atatctgata
    30361 acaaaagctg attttctcgc atgccacaag ttcagcttta ttgaaaggat agacatgctc
    30421 gccaatatca aagttggcgg gaccttcctt ctaaatagcc cttatcccgc cgatgaagta
    30481 tgggacaatt tgccaataga agtacaaaag gagatcatcg ataagaagct aagcttctat
    30541 gtcatcgatg ctatggccat agccgataaa gctggaatgg gcacgcgcat caacactatc
    30601 atgcagaccg catttttcaa gatctccggc gtattgcccg aagaacaagc agtcgagctc
    30661 attaaaaagt acacgaaaaa gacctatatg cgaaaaggtg ccgatgtcgt ccagaaaaac
    30721 ctcgatgcaa tagatctagc actgaatgct acacataaag tcaagattgg ccaagcaaat
    30781 agcacgaagc acatgctccc accagtgcca gatacggctc ccaagttcgt acgcgaaacg
    30841 ttaggcgaga taattgcaca acgcggtgag aaaatgcccg tttcaaagct gccactcgat
    30901 ggaaccttcc ctaccggcac aacgcaatac gaaaagcgca atatcgccga aaagatccct
    30961 gtatgggatc catccctatg cacccaatgc ggcaactgca gcctagtctg ccctcatggc
    31021 gtaatccgca tgaaagctta tgatcctgaa atccttaaaa atgcgccttc taccttcaag
    31081 agcgtcgacg gaaaaggcaa ggatgttgcg ggtctcaaag tcaccgtgca agttgcgccc
    31141 gaggattgta cgggttgcgg agcgtgcatc aatatctgcc cagctatcga tagagccaac
    31201 cccggtagaa aagcaatcaa ctttggatcg cagatcgagc tccgcgatac tgaaaagcaa
    31261 aactgggact tcttcctctc cattcctgag accgatagca aattcctcaa cctctcgatt
    31321 ccaaaaggca tagcaatgaa gcggccgcta ttcgaattct ccggagcatg cgcaggctgt
    31381 ggcgagaccc catatatcaa gcttgtaagt cagctgtttg gcgacagagc aattatagcc
    31441 aatgcaactg gctgttcctc tatctatggc gggaatctgc ctactacgcc ctatactaag
    31501 agagcggacg gcagagggcc ggcttggtcc aacagcctat tcgaggatgc tgctgagttt
    31561 ggatatggta tgcgcctcac ttcggacaaa cagaacgaat atgctcaaca gcttcttgct
    31621 tcggctaaag gcaaaggcat tcccgaagaa ctcgcagaca ggcttctctc caatactcaa
    31681 gagagcgatg aacagatcga agctcagcgc gtcgatgtag ctgcactcag agaatgcctt
    31741 gctggcaaga ccgaaggctg ggctaaagaa ctcgacaatg tagctgaagc gcttatccgc
    31801 cgttctgtat ggattttcgg tggagatgga tgggcttacg acataggttt tggtggtctc
    31861 gaccatgtta ttgcctcggg caaaaacgtt aatttgcttg tgctggatac cgaagtctac
    31921 tccaacactg gaggccaggc ctctaaggcc accccaatcg gagcaatcgc tcgctttgcc
    31981 acagcaggaa aggagacttc caagaaagac cttggcatga tcgcagcaag ctacggttat
    32041 gtctatgttg cacaaatcgc attgggagcc aatatggttc aagcaatcaa ggccatccgt
    32101 gaggctgaga gctataatgg gccttctatc gtcatcgcat attcgcattg tatcaaccat
    32161 ggcatcgata tgatgaaggg tatggatcaa cagaaaatgg ccgtccaaag cggtatgtgg
    32221 ccgctatatc gctacgatcc gaggcttaaa gctcaaggca agaatccatt ccagcttgat
    32281 tctagagagc ccgattattc aaagctcgag caatacatgt atgcggaagt ccgcttcaaa
    32341 agccttgttc aagctagccc agagcgtgca aaggtcctgt tcgagaaaca gcgcgacctt
    32401 atcgaacgcc ggtataagga gtaccgctat ttggcggaca ggcctttcta atctctagaa
    32461 tagtttttat cggataggct ttgcttctga atacgaaagc cacctcgcat cgaggtggct
    32521 ttttcaattt ccaatattga cctttgtggt cattttcttc tagagtggca atactcgatg
    32581 aatacaataa aatcatataa gtggaccatt gctagcgcaa tagcagtgct cgttctgctt
    32641 cttataccat cccaagcatt tcaaaaagca cctcaaagcc ttattggcct tgataaattc
    32701 gtacatgcga ttctttttgg agctatgaca gcaatttttt gtgctgaaca tagggctcta
    32761 aaaaaaatga acccaaaatt tcttttgagc cttatcataa tcagcgcttt tgcatttctc
    32821 accgaaatca gccagctcct tacaataaca agacaattcg atttaaagga ttttggagct
    32881 gatacaattg gaattgctac agctcttgta gtcatgcgaa ttgcatcact actttcgtga
    32941 gtttttcggt aattcgatgt aatattcgat agcaattaga aaatataaaa tgctttactt
    33001 aaggggaatc gctatggaaa acattgaaaa acaaaattcc tctgctcaaa aaaatgatgc
    33061 gactcatgct acagaaaagt tctcagaaat agattcatct actatgttgc atctgcaaaa
    33121 atccagcttc ctgtttttct taacaacgag cattttcctt gttttctgtt tcatattgac
    33181 gacattgatg cccggaatcg attttctatc cttctatccg ctggaaatgt taatacttat
    33241 gggtttatta acttttggtt ttctaataat ccatctgaat agaaaattgg cttggttaga
    33301 gcccgctctt gttatagcta ttctacctct gactctccta aagtattcta cttctattta
    33361 cagcatgtgc atatttatta ttgcagttat cgaactcagt cgcttgggtt attttaggcg
    33421 tgcaaccaca atcaaagcct gtatccttgt ctcctactat gccttgagtt tagtgttaat
    33481 agccattgcg catagagaat tcttcctcga cattattatg tcgattatct tcctatctct
    33541 gttttttata tattttcttc caataattca agatagatgg cagattggca aaacgaatcc
    33601 gatctataaa atcaacctaa gagatctaaa tctttcatgc cgagagatgg actttctaag
    33661 agattgcctt cacggagcta ctttcaaaga aatagcttat aagcaccatg tcagcgaatc
    33721 gaccgttcgc aatacctttg cccatatcta tcacaaattc aacgcagcag acagaatgga
    33781 ccttttgtca aagcttgctg cttttgatat catcgaataa ctcaacagag gaatttatgg
    33841 acttagattt aatgcctctg cgggatatag ctggagaaaa tgcagatagt attctcgcag
    33901 atgccgacaa gaccgcaaag gcttttaagt atttttatgc tgtgatagca cgccttcgtg
    33961 catccgatgg ctgcccctgg gacctagagc aaacgccatc gagcatacgt ggtaacatta
    34021 tcgaagaggc atacgagcta gctgaagcca tcacagaaga tgacaacccc catataaaag
    34081 aagaaacggg agacctatat cttcttgcca ctatggttgg ctatataagt cagcaggaag
    34141 gacgtttttc cgtagctgat agcttgtgcg atgaagcttg taaacttatc cggcgtcatc
    34201 ctcatgtatt tggtgagtcc gatgtcaata gtccagatca agtagttcag caatggaacg
    34261 aaatcaaaga gcaggtcgaa ggccgtagaa aaaaggagtc tatcctagac gaagttaatc
    34321 gtaatttgcc gccattagaa aaagcataca agcttcaaaa aaaagcgtca aaagttggct
    34381 ttgattggca gcaaaagcaa gatatctgga acaaactgga agaagaacta gaagagctaa
    34441 aagaagctcg agtcattttt tccgaagaat gcgaatccgc aaaagctgtg gataatatat
    34501 ctagaaaaga cgcactcgaa gaggaatttg gagaccttct ctttactgtt ataaatgcgg
    34561 ctcggcttta cggcatagat ccctctatcg ctctacatcg cacaaatgaa aaattctcgc
    34621 gacgattccg acatgtcgaa gagcgcatga aaaagcagca tctcgagatg aatcaggaga
    34681 atatgaataa gatggataca ttctggaacg aagcgaaaaa gggagaaatt ataggaaaaa
    34741 acaattagct atccgatata gtatcgagta gaacatcatt atacgatagt atatattcga
    34801 caatatggcc aatctgctgt ctgctcagaa gcttgcgaag gcgcgtaaga cattcaatat
    34861 ttacaacttt ctaaattcct tctcttttgt ttttgtctcg ggatccatta tcacgctctt
    34921 tgccttaaaa ttgggtgcgc agaatagcat catcggccta ttgaatgcct tagcctatgt
    34981 tacttttttt atgttgccat tgggcaagcg cttggtcaaa cggtcttcga tcataaaggt
    35041 attcggctgg ggatgggttg tcagatatat tgctctcgtt cccatcctat ttgccccctt
    35101 gttcgcatcg aaggggcata tggggcttgc gattctgatg ctaattttgg ggaccgcagg
    35161 atttcatata gcccgaggaa ttgccctcat cggaaacaat ccagtcttgg gactattatc
    35221 gactggtggt ggcgacaagc ctcgaagcga ccagggacgc tatctggtca ccatatctgt
    35281 tgtcaattct gtagcagcaa ttctagcaaa catagtactc gcttttttgc ttggccaaga
    35341 agcatcgcta tggacctaca caattgcaat aggtcttgga attataacgg ggcttatcag
    35401 ttgtgccata ttattcaaga cgccggaacc cgaggcattt tccagcaaag aagagcaatc
    35461 tctatgggat gtcacaaaag aagctcttgt ccagccggaa tttcgtgcat tcatatttac
    35521 cttcatgctg ttatccttcc tttctggcat ggggcgtgca tttctgccag tctatgcaaa
    35581 agaagtcttc gcgcagggcg acgatgcaat aatggtatat tcaattgttg caagcctcgg
    35641 ctcaataacg atgggactta taactcgcct cgttgtggat cgccttgggt cgaagccact
    35701 tttcataatc tatagcgcaa tcggatttgt ctcctttctc ccaatagccc tcattccaga
    35761 ctcggcaacc ttgatgggcg ctccatcggt tatcgcgctg tttctggcta tagttcattt
    35821 tctctcctct tttggatttg ccggcgaaga gaatgctgca caggtctatt attttggtct
    35881 ggtgccaaaa gaaaagatgc tcgatttggc ggtaatttac aatctagcct atgggattgg
    35941 aggatcgctg gggtcattcc ttggaggtgg aattctcgaa atcttttcga atataggatt
    36001 atcaaacgct ggagccttta gactatatta cgcggcaatc agtatagggc ttgtagttgt
    36061 tatttatagc atgcgaaatc tgccacatat gggcgcaaaa tcggtaactc aatcgatatc
    36121 tattctgctt tctccaaaag aactgcgagc tttcgaccta ttgacgagac tcgaccagag
    36181 cgcaagtgct cacgaagaag ttcgaattat acaaaaactc agcaagtcgg aatcgaacct
    36241 tacacaaaaa gaacttgtag agtatctcta ttcccctcgt ttcgaagtgc gatcggaagc
    36301 tctacttgcg ctcgaatcca tacccgagct cgatcacgag acaattgccg ccttgatacg
    36361 ggaagtaaaa gccaatgtct ataccacagc ttatgttgca gctcgaattc tgggcaaaca
    36421 acgatccagc attgcgattc cagcacttat cgagtcgctg gatggaccag actatatgtt
    36481 gcagagtatg gctatggtag cccttgctag actcgaagcc tttgaagcaa ttgaaaaaat
    36541 cgaacggctt atacaatcca cgaacaaccc acgagttcga atatcgggag cgtacgcgct
    36601 cgagcttttt ggaagaataa actcgatccc tacactgata tcttgtctga aagctgagga
    36661 tagaccagcc aatgtatcag acgaactcgt attgagcatt tctggcattc tgggcatcga
    36721 taaagaattc tatcttctat attcggaatt catagatgac aattcctcgg gcattgcaca
    36781 gctaatagca tacgcagagg atctgaatct cggagatgat agattgaatg cgtggatttc
    36841 agcagtggat gctctatttg ccccagattc cgatactgct ccccttgctc ggctgcttct
    36901 cgcaagccct aaaataacag actctatcct tatgctcagc gatggacttt tggaccccaa
    36961 gcttacatat ccaggcttca attttctatg tgcctatatc gctctacata tagaagatct
    37021 aaaagactat tagagatgtg ctgtaatctt agccgtaacc taatcctaga ttggcaggga
    37081 catcaaaaga gatgtaagaa gccttaccag aagccctccg agtgcgcgaa ccacaagaac
    37141 cgcaacaaag cctgtgatga gaccttgtag atattgtacc gctcgtccgc gatagataat
    37201 cttgtttatt ctgaaaagaa gaggattgat aattccatcc agaatctgcc aaagaggatg
    37261 aagcacggct atccgcgcaa ggaaacctat aatccgaata accgaaatca caaggaaaaa
    37321 acccaataca aaagatattg ccgaccatgc tgcatccaac aaaaggctta gaataaaacc
    37381 aagcgttatg cgcacagcat aagagagtgt catgaaaaga ttattgagta ccgacaacac
    37441 tgcgagcgct actatagggc taaaatcaac agctgttgtg cgaaaaagcg aaaaaccgcg
    37501 aaaaaggttg agatacggat ctgtcaaacg agcaataagc ttgccccatt ttgtgtcagt
    37561 gagcgaaggt atccaggaga aaaatattcg aattatacat aataccatgt acagcgatac
    37621 gagactgctc actatttttg caagagttga aaataccatg aattatcctc cgcacatcgg
    37681 ttctttattt agaagcttct ttatttagaa actgatacct actatactaa aaattgatgc
    37741 acataggcaa tgttcatatt ggcacctgcg gtttctccta tgaggaatgg aaaaccgtat
    37801 tttatccggc agatctccct tcgaatgaaa tgctgcgata ctattcgctt gtattttcct
    37861 tcgtagagct ggataataca tggtatcgca tgccaaatcc caaccagcta caacgtctgg
    37921 cgcttatcac ccctagcgat ttccgcattt cgttaaaagt gcatcgcagt cttactcatg
    37981 aaatcgatga aagttggcaa accaaagccg atgaattttc tctcgctgtc tccgcactct
    38041 cttcgacagg gagactagga tgtgtccttg tgcaacttcc atatcgtttt tcctatacga
    38101 tcgaaaaccg caaatatctt gcaaacctgc tcgctgcgct ctcgaacttt cctctggttg
    38161 tcgagttccg aaatgctaca tggtatcaag atagggtttt cgaaacactt cgaaaactga
    38221 aagttggact tgtaacagta gacaggcccg acttgcctgg cttacctccg gaatcagtta
    38281 tcataacatc ggacatttgt tattaccgtc tgcatggtcg caatacagac ctttggtggt
    38341 ctggcaatgc aaagagtcgc tatgaatact gctattctga acaagaatta aaggatcggg
    38401 cccgactcat acgtgccatg gcaaagcaga gcaagactgt atttgttgca ttcaacaatc
    38461 attcaagtgg ctatgcaccc aagaatgcag cctatatcca atctattctc gctgaacagc
    38521 aattgtgaga atattcttgg ctcatggttg attgctatct aattctatgc tttcctcgga
    38581 caggtctttc ttgatttctt ttgcggcaaa ggcttcgatc tgcttcgaga gctgctccca
    38641 gtcatctaag gtcgaatctt ctgcagtgaa gcagccatcg atggatggca caaagagcac
    38701 catttttcga ataagggatc tattgatttc aaggagctgc ttcagggctt cgcgctggcg
    38761 attatcgagg atatcgcggc ttatgacttc gattagccct tcggaataga gtgtcaccga
    38821 tagccagcca cttttttcca caaaatcgat tgtatgaaga gctccaccag ctattccggg
    38881 gtgcgaagct agccagctct catgaaatga aggtacggca atagtacggc catcggcaat
    38941 cacccaccat gcaccagtcg aaatctcaaa tggttttctc atcgctttgc tctgtatacc
    39001 ttttatcgag tattctgcct tcgcgctcta aaatctccct aagagagata aaatgaggag
    39061 gttcagccgc aagaattggc atattgggcg gcttaagaga tggccaaggc agtacgagtt
    39121 cataggccca taacatgaga tgtcttattc cttttttgcg tagagcttta ttaagcaaaa
    39181 aattgccata cttatcatcg ccgagaacag gcaggtaaat acccgccaaa tgccgcctaa
    39241 tctgatgcat gcgccctgta cttaactcta gctctaaaag cgaaagcgtc aaaaaattgg
    39301 cttctgcagc atcgacttga acatgagccg gatactgatt agctaacatt atttcattgc
    39361 ggacatgctc tttgcctatc gtatcaacag ggttattaga gggggggcct tgcaggggaa
    39421 ataaccaggt ctctcgcaaa gtccaaatag tacaggcagc ctgagctccc ctttttcctt
    39481 caagggcatc ctcaattgtt cctttcggct tagatggcac tcctgttacc caagcttgat
    39541 agcgtttggt tatctctcgg cttgcaatgc gatttgacca tttattggca gccttggcat
    39601 cttttgcgag catcatgcat cccgcggtct ccctgtcgag cctatggact ggaaaagccc
    39661 tgtatccgag ctggcgctct aacgctgcaa ttacgttatc gcgtagacct tgtcctggct
    39721 gggctgcaag ccccgctggt ttgtctatta cgatgatctc atcgtcttca aataatatgc
    39781 gaatcatcga tgctccatga taacagaaca atcgactttc agcaaagccg aaactgcaat
    39841 agagcgcata ataaaacgtt tgacgcctta acaaaaattg agaaaaagtt atgcttcgcc
    39901 ccattagcag gcctaaaaat gatgtgttgc ttgcatcgag cgctctaaag cggaaattgc
    39961 gctgtcgaga tcataagaga taagaacatt aaagagaata tcaattgcgg cgagctgact
    40021 aattctcgat agctcggccg cactgcgata gattctctcg actgtaggta tttcgagaat
    40081 aagatcagaa taatcacgta acctattttg accggtcatt gtcatcgaga tgagaaaaca
    40141 tcctctggtt tttgcttgag ccgcgacttc tatcatctcg ttggtctctc cagaataaga
    40201 tattataaaa gctagatcgg attgtctgag agaacaggag gctgtaatct gcgcgtgagt
    40261 atcgaataga tatgaactgg gtataccaat tcgcgtaagt ttctgatgaa agtcctctgc
    40321 aaccagaccc gaagctccaa taccaaatat ggcggtcata ctggctcctc tgattcgatc
    40381 tgccgcttct tccaccacta tcggatctag cgtctgggca agagaattca tcgtacgtcg
    40441 gactgcttcg gtcatatcac gaatagcgcg ggcgccagga gtctgcgatt ccagctctag
    40501 atcgggtaga aatttttcgc cccaaccttg gtatacatct tttgcaagcc agagcttgaa
    40561 atcgctgtac ttgcctacgc ctatatgacg acaaaagcgc actaccgccg cagggcttgt
    40621 gccagcttgc ctcgcaagct cgcttatgtt gagatgcaag gctttctgag gagattcttg
    40681 aacgaaaacg gcgatctttc gctctgcctc cgcaagattg ggaattgcgg cttcgattgt
    40741 gtctagcgct ccttgattca tttctattcc cctttgtttt tctgatcgct ttctcccttg
    40801 tcttttgaat aaatgcgtat tgcctcggaa agctgcccgt cgccgcgctc gataagctcg
    40861 gccgcttggt ctgcacctat gccaagtatt gtcataagta tagctatctt cggcttgcct
    40921 ccggaagcta ggaatgcggc ttcggcttca tccttagaac agccggttgc ttgtctgata
    40981 agacgcttcg atctagctat gagcttgcga tttactggca ttaagtcgac cataaggttg
    41041 ccatagactt taccaatgcg aatcatacta gcggtcgtta tcatattgag cacaagtttc
    41101 tgggcagtac ctgccttcat tcgggtagaa ccggtgatta cttcaggtcc tacttctatg
    41161 agtatcttat gatgcgcaat ttcaaaagtc ttcgactgcc gattacaaga gatcgctgca
    41221 actgctgcac ctaaattcct agcgtactcc attgctccaa gtacataagg agcggctcct
    41281 gaagcggtaa tacctacaag cacatcttgt gcagaaaaat caatatcttt gagagctttt
    41341 accccatttt ccggatgatc ctctgcgcct tcgatcgaat ttctgagagc agcatcccct
    41401 cctgctataa gcccaacaac catgctaggc ggaactccaa aggtcggcgg acattctgaa
    41461 gcatcaagta caccgagcct tccagaggta ccagcgccta tgtatactag cctcccatca
    41521 ttattgaatg cccgtacgac atcctcaaca agcgctgcaa tgctaggcaa ggccttttcc
    41581 actgcaaagg gtaccccttt atcttcttcg ttaataatcc tcaaaatctc tatcacggga
    41641 agatcatcga tatgttccga atgcacattg cgcgattcag tagtcgcgcg ttctaggtat
    41701 tcatcgaccg aatctatatc catggacaag cttctcctat taaaattatt tttcacattt
    41761 ttgagagaaa tgcaagctca ttcatctaaa aatagaaaaa ttatttcaaa ttatgattga
    41821 caacccgaat aaattcatca atactaataa aaaaccatgg atccatcaag aaaatggacc
    41881 tggaaatatc tatcaaggag gctagcgatg gcaaaaaaac tatcaatcgt attgctgctc
    41941 atagcaatgc tttctcctat gtatgctcaa cagaagacag tgagcatctg gggttggaga
    42001 gctcaagatg tagatgtatg gaaaggagtc gaggctgcac ttaaggcaaa gggtgagcaa
    42061 atatccatta gatatgaagt ttttccacct actgagtacg attccaagct attggtttct
    42121 ttgcaaggcg gagttggtcc agatatcatg tatacccgca ggcttccggg tgcgagaacc
    42181 caagccttgc tcgacaatgg ttatctaact gccataaacg ggctcgtcga tttatccaac
    42241 ttcgatgatg tttcgctcag ctttattagc acggataata aagtctgggg cgttcccttt
    42301 gcaaaccaga taatcggaat attctacaat aaggcaatct ttgacaaata caaactcaaa
    42361 gagccacaga cctgggacga gatggtccag attgctgaga cgctaaagaa gaatggcgta
    42421 actccattct ttgttactgg caaagaagct tggatacttg ctatgcagaa tgccatggtt
    42481 ggtgtgagct atcctggaga cgaatggatt ggaaaactaa ccgtaggcaa ggctaaattt
    42541 acagatccag agtatgtaaa tatgctcaaa gacctcaatg cccttaaaaa atactaccaa
    42601 aaagatttta tggccaatac cagcgcagaa caagatgttg cttttgccat ggaacaggcg
    42661 gcaatggtct tctatggcgt ctggggcaat acaaattggc tcaagatgaa tccagaccta
    42721 aagttcgact acttccctat cccaccaaaa gataagaatg taccagcaag agcatataca
    42781 tatatggatg gtgcatacgg cctaaataat acttcgaaga acaaagaagc cgctttgaaa
    42841 gttctcaatt ataccggtac caagacttat ggcgagctgt tttcatcgac aactggcgaa
    42901 atcacggcta taaaaggcgt cactatgccc gcttcgaagc cgatactggt aaaatgctat
    42961 gaaaaaatga caactatagc cgcaaagaac cgctactggg taggttctcc cttcgatgca
    43021 ggaactccaa gcgtctacaa cattctagaa aaagacatgc agtccatgta tcttgatcaa
    43081 atgacaccag aagctttggc aaagagattg caggatggca tatctacatg gtacccagct
    43141 ttcaagaaat aatatttctt gataatcata tgtttagtgt agctttgtga aaaaagtgcg
    43201 ccgcggcttt attgagacaa gggccgcggc gcttgtctca ataaagtcgc ggcgctttta
    43261 tatagcgtgg gagagaggtt gctgtgcgaa aatattcttt tctttggatg cttctacccg
    43321 cattcattct gtattcggtt tttatagcct atcctttctt ctcgagcctt ggcctcagtt
    43381 tctatgattg gccaggaatt ggcccaaaga aattcatagg tctggcaaat tataaaaata
    43441 tactctcagg attcatgtca tctgagttct acaacgcgct gtggcacaat gtcgtttttt
    43501 ttatttggtc gctcattcta tctgtggtcc ctgggctatt ctttgcattt ctgcttgctg
    43561 ccaatatgaa aggaaccggt tggcttaaag tagtcttctt cttcccaaat acacttgcaa
    43621 ttgtggtggt tggatttctg tggggactac tgctcaatcc tcaatggggc cttataaatc
    43681 agctctttag ggtagtcggc ctagacttct tggcaaagcc atggcttgga gacacaaaga
    43741 ctgcactgcc tacaataatt tttgttacgg catggcgagg cttgggattc tatatactca
    43801 tatacctcgc agcgattttg ggtgtcgaca gggaaatgat agaagctgca cgcatagatg
    43861 gagcttccga gctgcaaatc gcaagtcgta tcattctgcc tcatctctat tcgataatcg
    43921 cgacgacatc catgctcaaa ttcatctgga cattcaatat cttcgacatt gtatatgcga
    43981 tggaaggcac gcagggaggc cctgctggtt ctacagatgt tcttggaact cttttttaca
    44041 gaattgcatt tggagggctt ggagcaagcc aggttggtat gggacttgga gcaaccgttg
    44101 tgaccttgat attcctcata gtattgccgg tctctatatt ctacgtcttt atagtggaaa
    44161 agcgtgtaga aaagggggaa taatgctcaa gttcaatggt tttcagaagg tgcttgcatg
    44221 gatttttctt cttttactcg cgtttgcaaa tctatatccc atcggaatca tgatgctgtc
    44281 tagctttaaa tcaacaaaag aaatattcct cgagccattt aatcttccga gcgtctggag
    44341 atggcaaaac tatgtctctg cttggcaacg cgccgatttt gcgacttatt tcaagaattc
    44401 tgttctcgta acggctttgt cgattgtctc catcattatc gtatcttcga tggcagccta
    44461 tgtaattgcg cgcttcgatt ttaaatttaa gcgaggcgta tatctctata tgctagcagg
    44521 acttgcgctg cctactcgcc ttgcgatcat cccaattttc ctaataatgc gcaatcttca
    44581 tctattggac aaactttcag gcttggtgat tgtatacact gccagcggga ttgctttttc
    44641 ggtttttttg ctagtgaatt tttttaaaaa ccttccacgc gacatcgaag attcggccaa
    44701 aatagatggc gcaggacctt tccgcatcta ttggcagatc gacctaccgc ttctaaagcc
    44761 agcgctggta actgtagcaa tgttcaactt tatcgatgtg tggaacgact tctttttccc
    44821 tttaatcctt ctgagttcga aagctaaaaa gacagttccc ttggggctac aggcattctt
    44881 tggcgaatac acggttgaat gggatgtgct ctttgcagcc ctcaacatat cggtcttgcc
    44941 aattctaata gtattcctaa ttctgtcaaa gcaatttata gcaggtctga cggaaggtgc
    45001 cttgaaataa gggaaaagtc agatactagg gctttgaagc ggataaacaa caagcaaaac
    45061 cccagcaacc agcagattag cccttctggg ttccatatct aagatatttt tctctaatcc
    45121 ttagaaattc tttctcccct ctgtgcgcaa attcgagaca gtcctcgaga ggtgctccgc
    45181 ttattatcat gaatctcagg gttgtcccac cagctagctt atcgaaaaag ctaagctctt
    45241 tatcgtccca atcctctcgc catcgaaatt catttggata cattcgatta attgtatgga
    45301 ttaaagctat gcctgtatcg agcgatcgca gcgcttctct atccgtaagc gaaattagga
    45361 cgccctcaca aagctccccc ttatatttag aggccgtagg cgaaaaaaac agggagatga
    45421 acactacgcc aggcaattca agagcggcaa gctgttctct cagagcctct ccatcgataa
    45481 aaggcgctcc aataatctcg aatggccgtg aggttccccg tccttctgaa agccaagttc
    45541 cctctacaag acatgtacca ggatataatt ccgcacagct gagagtcgga agattaggcg
    45601 atggcaatgg ccagggtaag tttgtttcta tccatgccgg acgagaatcc cagcctgaca
    45661 tctctattaa ctccagtcta cagttagggt agtactcacc ctgtagatat agagcaaatt
    45721 ctccaattgt cattccatag cgtggcggaa ggccatatcc ccctacaaag ctttccaatt
    45781 ctttaggaat aggagaacct tccaccttcg atcctcctat aggattaggt ctgtcgagca
    45841 cgataagcgc tgtacccact tttgcgcatg cttccattat ataagcaagg gtatagagat
    45901 aggtataata gcgacagcct acatcttgca tgtcgaatac aagggcatcg agagattcga
    45961 gcatccaggg ttctggccta aggcgctttc catagagaga atatgcagga atgcccctga
    46021 aaacttcatc gtccagctct actgcgtcct gtgctgcgcc cctgaatcca tgctctggac
    46081 cgaagagaca cactgggata attccatgtt ccatgaaggc tttccaggaa ggtatgcctc
    46141 tagatgttat cgccgcaggg ttggtagcta gtccatagcg tttattatat ctacgcggat
    46201 catccaaaaa atgttcgaga ccgaatacaa ccgaagcctg ttgctgtttt gacttgatca
    46261 tgctatatgc taacatattc atatatgacc tacaacagca acaaaggctc acaaaaaagc
    46321 cacaaccaag ccgaagaaac tctcgagaac cttctcgata acgcaattgc taaagacctc
    46381 tgtacgggca ttgcggcctc agtttcaata gatggcagac tcctctggtc tcattgttct
    46441 ggatttaccg caagagaaga actagctctt tccaaaccca tagaaaacga cggcaaaact
    46501 tctaattcta tcattgctcc catcgatcca gagactcgct tcgacgttgc atcgatgacc
    46561 aagcctttaa gcacaagcct tcttattctg aaagcactgc aagctggggc attgaacctt
    46621 caagattcct taggcaaata cctaccggaa gtaaattaca gaacaggaca aatcccgcta
    46681 ctcgctcttc ttacacacag cagcggatta cccgccattc cggccttgca tagctttttt
    46741 gctgattcag gtagactcga tcggagtctg gctttggaaa ggcttttttc gatcgaacct
    46801 caatttgcca ttggaaagca tgtagtctat tcctgcactg gctatatatt cttgggtgcg
    46861 ctcttggagc gcatttctgg gatgcttctt ggcgagcttt ttcaaaaaga aattgcagct
    46921 ccactaaagc ttgaacaagc ctctttcgca aaaaagatac agaaatcagg ggagcctgag
    46981 cacctcgaca atgccgttgc aacagaatat tgcccatggc gcaatagaag aatacagggc
    47041 caagttcacg atgagagctc ttattgcctg ggaggtcatt cggggaatgc cggacttttt
    47101 ttgtccttaa aagatgcgat tactctgggg aattttatta tgcaggaagg aactttcggt
    47161 ggaaagcaga tattgaagcc cgaaataatc cagctattgt ggcgagaata tacccatggc
    47221 ctggaagagc gtcgaagtct tggttttaag cttcatgact ccacgaccgt agatggtcca
    47281 ctttggcctt tacaatcctt tgggcacacc ggctttgttg gcacaagcgt tttcttcgaa
    47341 ccgcagcgaa ggcttttcgc aattctttta accaatagag tctattttgg gagagagcaa
    47401 accgcagatt cgatccttgc gttccgaaga gaattttatt caagagtctg gcatgctttt
    47461 gccgaataac caagacatac ggaaatccat atagagcatt tcgtgaaata attttttata
    47521 attagaattt tttctttatt gacatcttaa aaacaacctt tataatgaaa atatatttta
    47581 gggcgtttgc caatacttac aaggattcct gaagaattac attagcaaaa tttctgcaat
    47641 tttcttaaaa attcaaatgt gaacgattct cggctttgcc gagagataga ttttatctgg
    47701 attcaatcta gagattgaaa ttctatagga ggtatctatg aaaaaggcaa ttgcattatg
    47761 tgtgctggcg atcgctattt tagcatcggt gccagcgcag gctccggtcg tgcctggtct
    47821 tggcagcaag aaaataacca tcaatttatg gactcatgag gatcccaata ggtctgttat
    47881 agaaaaaaaa tacattgccg agtttatggc cgccaatccc aatatcaccg taaactataa
    47941 tacctatccc tcgaatcgga tattcgaggc cctaaccacg gcttttgccg gaaagcaagg
    48001 cccagaaata ttcaatgtct ctataggcaa tgcagtttca tttatcgaca atggaagagt
    48061 tgctcctgtc gactacaaag ccctaggtct caagggtccg gccgatcttg aggctatgta
    48121 tcttccaggt atgctcaagg ccgtcgagcg cgatggcgat ttttatggac ttcctctaga
    48181 aatcaccaat atgtgccttt atgtaaaccg caaaatgctc aaggaagctg gcatagatcc
    48241 cgataaggac tacccgaaga catgggaaga cctcattagc ctttccgaaa aaattgttaa
    48301 acgcgaaggt cagataataa cccgccgtgg cttcgatttc cgctacgatg cgtatacgaa
    48361 cctcttcatc cccatggtcg aacagctagg aggagaatat gtctctaaag atggcaagaa
    48421 ggctatcatc ggcgacgaag cctggctcaa agcccttcaa tggatgaaag aactagggcc
    48481 agccggaaaa aatcttggtt cgccaaccta tacggcagct cgaaccgcat tcgacaaaaa
    48541 tgacaatacc gttgcaatgt ccttttccgg cctctatcaa gaagctcgta tgaaagcctc
    48601 gaacccagat ttctataatt cgaaagattg gatggtggtt cctttcccac aatggaaaga
    48661 ttcaaaaaag aaagttacag ccaattactc cgcacattac tacatggtga atgcacaatc
    48721 ggataagcta gcccagcaag catcgtgggt attggtagga tggatgctga gccatggaga
    48781 agagtatcta gacaaagtgg ctataatcca gccaacaaag aaactaatcg agtcgccaat
    48841 atataagagc atgccatatt ccgatgtttt tgctaaagac tttgaaaacg cgaatattgt
    48901 ctatgttggc aaggcaagtg cgcggctttc gaccttgctc agtgagacca tccagaatgt
    48961 aatggtcggg ggtgtcgatc caaaggtagc tctagaaaaa ttgcgcaaga gcgcacagga
    49021 agcactcgat tcagctgatt gaattgattc ttcgtcctga gagaccaaaa tgaaaggaca
    49081 tagatgatgg gagatcgtcg aaatcctcta gaagagaagc gcaacagatg gggctggttt
    49141 ttcgtagcgc ctggaatagt atttttttcc gccttctcgc tatatccaat tctatatgca
    49201 atttggacta gctttcataa caagaaattg ctttcgctca agccacctaa atttattggt
    49261 ttgcagaatt ggatatcggt tctgagttcg caagattttt ggaattccgt acgagcttcg
    49321 gctgttttct gtataggcgt tttcattccg gtatttttag gatccttact gtttgcgctt
    49381 ctaatctcga gccgcaaaaa aggaaagaga ttctaccaaa tggctcttta ttcgccggca
    49441 attttgtctt cggttgttac ggcaagcgtc tggctgctca tcttcgatcc tcgtggcctt
    49501 gccaatcagt ttctcaacac actactgggc acagcagggg ttgatcacaa gtggcttgcg
    49561 gatcccgtga tggttcagct atcgacaatc ctggtctatg tctggaagca agttggatat
    49621 tataccatcc tttttggaac aggaatcgga aagattcctt ccagcgttgt cgaggcttca
    49681 atgatagatg gggcaaatag ttggcagcga ttgactcgta taaccctgcc tttattgaga
    49741 cccacgatgg cgttggtgtc agtgatgatc tttatttcga gccttaagac cttttccact
    49801 caatatcttt tcaatcaaca tggcgcacct ctagcgccga tcaatgtgct aactcttaat
    49861 atctaccgta cggcgctctc cgatcaaaat cttggcaagg caagcgtaat gagccttttc
    49921 cttttcttca ttttattggt cctttcatgg cttcaagtaa aagccggagg tcaaggggag
    49981 gagcttgaat gaaacatgaa acttccaagc tgagctcccg ctggaatacc atcaagtata
    50041 atgcaggaag cttcatagct accgcaattc ttgtaattgc atgttctgcg gcaattctcc
    50101 cctttgcctt tatgctcagt gcctctctta tgcctggtca cgatgttaca actatccctt
    50161 atcgctggat tccagaaacc cctctttggt caaattacct ccgcgctctc aaaggatccg
    50221 atggaagctg gatataccta cggaacattg ccaattcctt tatcgtcgcg tcgtcggtag
    50281 ccttgtcatc cgtgattctc gcctcactta cgggctatgg attggcaaag ttccacttca
    50341 aaggaagaaa tatagtcttc atgggaatca tggcgactat gatgattcct ttcgagaccg
    50401 ttatgattcc gctttacttg accgcggtca aattcggcct tcagaatcga ctgatcggac
    50461 tcatatttcc tttcctcaca agcgcttttg gtgtattcct tatgcgccaa ttcttattag
    50521 gttttcctga tgagcttcta gatgcggcac gaatagacgg ctgctccgag tttcaaatat
    50581 acaggtccat tgtatttcgc aatgcgacac cagctattgc gacattggca attcttgcct
    50641 ttcgcggaca gtgggataat ttgatttggc ctcttctgat aatacaaaag gaagagctca
    50701 aaaccatacc tctctatata gtactcttca cttcagagct tagcaccgac gagggcgcca
    50761 tgatggctgt agcggtcatt gcagcattgc ctgttttcat actcttcttt actatgtcca
    50821 agtatttttt aagcggcgca ggagtctata ctgggagcaa aatatgatcg acctttatat
    50881 tttcgacgag ggaggggtct taatacgcaa ccacatgata atgggcgatg tagcagctgc
    50941 cctaggattg gatattacta ctttcctcga gtttattgca cctgatataa aatcgctctc
    51001 gcgagggtcg atagacagcg ccgagttttg gcgtaggttt tctgatcgca cagggattag
    51061 gccttccgaa aattatctaa agacactatt ccggccgcta ccagatgaag ctactttctc
    51121 attagtcaat gaattgtcaa agaaagccag agtagtatgc ggaactaaca caatagatag
    51181 tcatcacgag atcaatctcc agcttgggtt atatagtccc tttcaagccg tatatgcatc
    51241 ccatattata ggccatataa aacccgagct cgatttctgg caacatatcc tcgcagaaga
    51301 aggagtatcc gcccagcgca cattctttac cgacgacagc gaggacaata taaaggcnnn
    51361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnata gataggatga tttttaggca
    51481 gggaaaagag aaatgcgggc ttttgtcttt gatttacaag aaaatcctgt tcatcctgca
    51541 aaatcccgta atcctgtcag gggtattttt gtcatgtgta tcctatttat cctgtaaaag
    51601 cttcgtagca ttattaaagg cttaagaggt ccccaatgaa caaccaatgg atttttggca
    51661 tagatggcgg cggtacagcg acaaggctga ggatagaaag cctagaaggc aaggtcttgt
    51721 accaagcaga aggttccagc ctaaaccctc gctcggtcgg ttggaatgga tcgagaaaaa
    51781 cgctagacga tctttttgga agtatgtaca agagcacaaa tctctccacg gatgaatgta
    51841 tctctggttt tgctggtgta gccggtatcg acagagaagc agatacgggc acaatgcgaa
    51901 gcatcattcg agaggctgga aatttggctt tcgataccct aatcgaggta aagaacgatt
    51961 cgatccccgc gcttgccggt gcctttggag agctacgagg gattcttctt atagcaggga
    52021 ccggatcgat tgctgtagga gccgatggcg ccggtcgaat catacgctcg ggcggttggg
    52081 gtcatatcct tggagatgaa ggatctgctt actgggtcgg ccttcgcgcc ctaaatgcgg
    52141 caattcgatt tcaggataga agagggcctc aaaccgatct gttgggcagg gcactcgctt
    52201 tttttggcga aaaagaggct ttttctctta ttccggccat ctacgaggat ttcgacaagg
    52261 caaaaattgc ggcatttgcc agaattgtag cagaagaaca agatcgaggt gacgaaatag
    52321 caagacagat attcatagaa gcttccgaag agctggcctt gcttgcaact tctattgcta
    52381 tccgactcgg agaggcacat agcggcggaa ggatcgcctt ttcaggagga tttatcacca
    52441 acaatgaaca gctttggcga gacacagaag cgcgaatact ctcggcactg ccgcggcacc
    52501 gagttgtagt acctcaagcc gatgcaacaa tcggtgcttg catcttagcc cgctcgatag
    52561 ccaacgccgc agctagataa tgcttatgag ctactagatg ctaagtatac accgccaatt
    52621 gcaaggcaag cacctgcaat ctgtatagcg ctcaggcgtt ctccaagaaa aacaaaggat
    52681 gcagttacag atactaccgg aatcaaattg atgaacacgc tagatctccc cggccccagc
    52741 tcttccagca cgatgatata gagccaatag cctatcgccg agccaaacac ggccagaaat
    52801 gtagcattca agaggaccga agccgaaaac ccagtccaag attgatgttc cgctaacgca
    52861 aaaggcacgc agcctagggc accgataacc atttgccaga atgttattgt cagcataggg
    52921 tagcgcccag atagcgggtt ggtgatgaat ccataggcca cccaggaaag cacggctccc
    52981 agcatgaaaa aataacctga cacagatgtc gaagcattcc ccgagcgcat cacaataagc
    53041 gctacacctg caaaagagag cacaattccc agtatgaccg agcgtcgagg ccgaaccttg
    53101 aatgcaataa tctccgcaag aagtgtgaca acaggaattg tccctacgat aagcgatgat
    53161 tctgatgcgg tgagccgcat gattccattg ttctcaaagt agtaatagag agttacgcca
    53221 gtaaaagcgc tcaacgagag catggggaca tctttccatg caactcgaaa actggttctt
    53281 gtcagtaaca ttatcaaggc cagcaaaaaa gatgcgatcg tgaaacgaaa gagaggaagg
    53341 ctcatcggcc caaattctcg caaagctact ttgatcgaaa ggaatgtcat gccccaaatg
    53401 acagcaacaa taacaccaaa aaacgatgcc ttgaaccttc tggttttcat gaccaatcat
    53461 gctatcggca taggcaatcc ctgtccatgg gtggtacttc ttatgctata ctcgaaagca
    53521 tgaatgaagt gatcgtcgat ggttgttcgc tttctatttc agccgttgtt gcggtggctc
    53581 gccacaaatt tccagttcga ctcgatccag atgccgaatt gcgtatgcag gcatctagag
    53641 aagtcgtgga gttcgcagtc aagaaaaaac aggtaaaata tggcctcaca accggttttg
    53701 gcaaattctg caatgttatt atttcggaag aagacaatgc gcggcttcaa aaaaatctta
    53761 taatgagcca tgcttgcggt cagggcgagc cattcgcgcc cgagattgtg agagccatga
    53821 tgctgcttag ggcaaacgcc cttgcggtgg gcaatagcgg cgtacgaccg gtggtagtac
    53881 agcgtatact tcagctcatc aatgaagata ttgtacctat tgtgcctgaa aagggttcgc
    53941 ttggcgcttc cggagattta gctccgctag cacatattgc actcgtgctc atcgggatgg
    54001 gagaagcctt gtataaaggt gcaaagatga ccggcgctcg tgctctatcc gaagccaata
    54061 tccaaccact cgtcctcgaa gcaaaagaag gacttgccct catcaatgga acgcaggcaa
    54121 tggccgcgtt aggtgctcta tctgtatacg atgcgctcct tctataccgc agcgcaacca
    54181 gcgcttctgc ccttactttt caggccttga gaggcataac agaagcactc gatccgcgca
    54241 tccacgaact aaggcgccaa aaagcccaaa tagaggctgc tcaagacctt cgcaggctgc
    54301 tggaagattc gcgtctcacg agtgcagctt cggggactag ggtccaagat gcctacgctc
    54361 ttagatgcat tccccaggtc catggcgctt gccttgcagc catcaaacat gtatgggaaa
    54421 tagtatctgc cgagctcaat gcggtgaccg acaatccgct cattttccct tatgtgctcc
    54481 gagagggcgg cgatattatt tctggtggca actttcatgg agaaccattg gccttagccc
    54541 tggattatct aggaatcgcc gcttcggaat tggctaatct ctcagaacgc agactcgaga
    54601 gaatggtaaa tccgactctc tccgaaggtc tgcctgcttt tctaaccata aatggcggac
    54661 tgaattcagg ctttatgatt gtgcaataca gtgcagcaag ccttgtatcg gaaaataaag
    54721 tactctctca ccccgcaagt gtagactcaa ttccttcaag cgcgaatcaa gaagatcacg
    54781 tatcgatggg cactattgcc gcacgtaaag ccaggcagat tatcgacaac acaagacgag
    54841 ttgtggccat ggaactgctt gctgcttgcc aagctctaga tctgcgcgct attcaaatcg
    54901 ggcttccttc tatagtagat tcgctatccc ctgccacggc tccaatatat ggcatcatta
    54961 gaactgtagt tcgttttgcg gaacaggatc gaataatgta cccagacatc gatgcggtag
    55021 ttagactctt gagcgacgaa attctacccc agactatgga aagatggagc tggaatcctg
    55081 aacctgttgt tgctcatgaa taataagtct aaaatctaga tgaccctttt attagaggct
    55141 ttttgcaagg agtgtcgctt atggtgacaa agcgcgatca cggtctacgt ctggacagaa
    55201 cttctccgca agagagagct cgacttattt catatattaa tatcaagctg aaaagtttag
    55261 gccttccggt atattccaaa gaaggaattg gattcgtaca gctagctgcg gacatgctag
    55321 agagtttcag gcagaagaat agactgctgc caaaaatcct tccgcctgca gatcagcgca
    55381 tacaaaattt catcgaccaa tatctagcag acctgggact tgctcgaatt ccgcagctac
    55441 cctcgaacac gctggttctc gatcactatg gcatggctcg cgagctttcc cttccacccg
    55501 atggacccaa acatgtatcc cctacgctga cctcgtatcg tgtccgcaac ggtgtcctgc
    55561 acaacccaaa aagcgaccga cgtacaactg aaggcgtatt tcatatagcc gaaggaggct
    55621 tgccaattcc gctcgacaag aaggcagtgc ctaagccctg ctttgccaag cttctcgaag
    55681 cggctctgaa ccctcctctc gatatgatgg aactaccctt taccgcagga gagaacgaaa
    55741 aagcgcatac tttcgcatca gtgttgttgc gacctattgt tcgtccggaa attcccgact
    55801 actgcgaaga gagatcgatg gaaatacgct tttttgctcc tggttcgctc gtagcgaata
    55861 tcgattttgt ggagagcata tttggaaatt ctggagatcc ttttattccc gaaaacgatg
    55921 cagcgataga tccagttcat tggacaggca ctacgggctg catcatcctt gctacacatc
    55981 tcacggagct cacaaagaag gaacttggac tacccacttg ggagcaagcg acagaacgcc
    56041 agcgccgcga cggtatgtgc tggcatgacc ccgccgaaaa gtacaacgat ggcaaaccct
    56101 tcaaaataac cgctcgcgat gaatccggag tcataattac gataattgcg gacaattatt
    56161 ttggctattc taaaaaagag ataaagtcgc atatctccta ctctgccaat ctcttaggcc
    56221 ttgccgaaga agaacacgcg ggcggagctc ttgtctttcc ttcctacaat ctgggcacgc
    56281 gctttgtacc agacacaaat ctaaaatcca agggccacag cctcaatgat gtctattcta
    56341 taatgcgtgg ccgcatcgac attaaacaag aaggctatgc tatcgacttc acctttccaa
    56401 atatcatcta tctaccagaa gatgcatata tttctctgga agaccagatg gcacattggg
    56461 tcagcgatgg catcgaagaa tctctacgaa ttcttccggg aaatatctat atacatccaa
    56521 cgggctatca gattcgcatg gaacagcatt ctgtggctgg ctcgtggcga ctaatcggca
    56581 cttccgcgga aggacttcta tgtcacaagc cctgtacggt atctgggggc ggcaagtctg
    56641 agatagcaaa atccatccaa gatgctattg tctatatgcc gatagtcatt gcggatttcg
    56701 ataaggacat gcaggaagtc aaaaaaataa tcgaaaaaga ctatggcaat cgtttccgag
    56761 acgaatcgga taatctagga aaagatacaa ggccggtgct ttctccaaag cgttcccttg
    56821 gttcggttat aaagctcctt tctccctcac ctgcatatag cgatgaatac aatgaatggt
    56881 tgcgttccat tccggaacgt ataaagagcc ttgtgttttt agtaaagcga ttctacaggc
    56941 ccgattgggg tctcgactgg atgtctcatt tctcggtaga tgcagtcaat ggtactactg
    57001 gaaacattct aaagttcgaa ggcaaaccca taatggggtc ttacctccga gtaggcaaaa
    57061 acgagaaggg tctgaaccgt ttcttcaaac ttaggcagga cttcatgcca gccttcaagc
    57121 tccaatggga agacgatatc accgcctcga taattgtgcc tgtaaatcaa ctagaaaatt
    57181 tgccagattg ggcttccgat catatgagcc tcaaatttgc caaaaattgc gaggcgcgtt
    57241 ttttccaacg gcctgatgat gccattattc gaggctatga taaacaaacc gaaaaagatc
    57301 tcactcgaca cgacaatttc ttgtcgaatt ttgagcctct cactcgcgcc gatgcctctc
    57361 gcataatcga aaacactata catttctccg aatacaccga acctatgcga aaattcatcg
    57421 aagatagcga aaaagacccg gacttcgagt actttgttgc ctcgaattat tttaggatcg
    57481 tagacgacct accttcaaaa aatccgcgct atctccaatt agacccaagc tatgcagatc
    57541 caatggcacg ctatctcgca gagcttggcc caagacttta tcggcgcatt cctgcggaca
    57601 aacctcttcc tcaacccatt ggtgccgtgt tgccaggacg caggaacaac ccgccagatc
    57661 gccaagcagg tatacgcccg cttgccgtat atggtccgat tcattaccag gaattgccag
    57721 agctattcat ggatttcgtt tgctctctaa ctggcaagag cccctcaacc actggagcag
    57781 gaagcgaggg agctcttaca aaggggccct ttaatgccct tgtacctact accgacctca
    57841 acaatgcgct cttgggcttc atccttaccg gttatgcggg tttcacaacc gccgctggcc
    57901 atattggtca caaatacaag atagatcatg atatatctct tatgatgcca gaactgtggt
    57961 ctcggctgag cccagaagag cgcgacccag aatttttaaa aaagaatg
//
LOCUS       DAOSOB010000004        56989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_611_length_56989_cov_82.058331, whole genome shotgun sequence.
ACCESSION   DAOSOB010000004 DAOSOB010000000
VERSION     DAOSOB010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 56989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56989
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_611_length_56989_cov_82.058331"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PLS88_00790"
     CDS             <1..671
                     /locus_tag="PLS88_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05928.1"
                     /translation="AYSQYILSLTHATLRRSGRCTDSQKRRKTILYRSLVFEKAALER
                     SFSELLDQLYRERCSAYSSPLILITDEKCEYARALKLHPSRFSIHHLTVNSKVPRTFQ
                     NPLFASNYLDRELRKDLADHRRETVCFPRNVANMLNRLVVYLGWHNYEKPYRIGRDIV
                     LTHAEVAGIEREAICKAREKQFQERAFLSRAGLSLLEKKLWLRSFPTPLKRKAEYVPA
                     YAYA"
     gene            1032..2336
                     /locus_tag="PLS88_00795"
     CDS             1032..2336
                     /locus_tag="PLS88_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05929.1"
                     /translation="MNVVPAVLALSKNLSTGRKIMKSFASVDATSTSIGGVLERFQAS
                     LGNRELSFSEFLSDAYVQKWKGEQLAHALKVRTALVLGLQEFLVSRGILNIDRVSLSP
                     VTDPLCQDVEHVPIILYQDTPYRTTHSMIYSKMLACLNPYIEGIYVDSPNIRLELPSL
                     NSRHKYLIDFSQMDIEFKRAHKLSEDDYFDRPKETAEILSKELDRALDFFEDLIIYST
                     KKILVLAAESLEALGVKLTVPSKPFPRFYSDEIEVQNGCSLEEQLGQKAKTQFFWVLG
                     LLRENYDLVYPYLSRDGRRPSKKEISPRQVFNYDLCAAPLYRDGSLGHAYEILSGGLR
                     EWVYEAIVERLLDNAVLRESPRFDRYGNLENMAALDGYGPFLSIAHMRDSQDQRFFPQ
                     VCGGGLGIERTLFALLQGPFIKDIDEVTCFGKNPDKTLPFLF"
     gene            complement(2435..3721)
                     /locus_tag="PLS88_00800"
     CDS             complement(2435..3721)
                     /locus_tag="PLS88_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HOU05930.1"
                     /translation="MESYDVVIVGTGPAGLGAAFELLESRPSFNILLIDKQRVSSGGL
                     RNDCKMNFTWPIGFPLECWDEATGKHYLERVEAFLEPHFMEKRNIEIYRKRAEKIGVQ
                     LLDVRQSHLGTDGGFKLIKDLTARLKELGAAFSFGEEMLRVEPRSRVLITDKRELQYD
                     SLIVAPGRGGFAFLQRLMEELGIKYRDNVVDIGVRIETTEEHYPIVSDYYDPKFLFPK
                     KTRTFCTNSRAAYVVQERYGDEKQGYWYSINGHAWSHERPPNGLVNFAILKTITLTQP
                     LASGQDYAQMLGHLAALLGGGRPIMQRIGDFRLGKRSFANSFTGDLYNFNPSLPSCTP
                     GDISLCIPAKTMRAIWNAMKLLDTIVPGVMHPSTIMYYPEIKLYANRPVFIDENFQVL
                     PGIYFIGDGAGTSRGITSAWASGMRAAHGIIRLSNN"
     gene            3967..5118
                     /locus_tag="PLS88_00805"
     CDS             3967..5118
                     /locus_tag="PLS88_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU05931.1"
                     /translation="MKRIVRIGLLTIGVALLIVACGPGAPKTVKFGVFEPLTGANAGG
                     GALEVEGIKLANELYPTVKIGDKEYKIELVIADNKSDKVEAANAAQRLVDKDKVNIVL
                     GSWGSSLSMAAGPIVKNAKVSAIGLSCTNPLVTKGNEYYFRVCFIDPFQGTVMANYAY
                     NTLGAKKAVIIREVSNDYSVGLAKFFEDAFKQLTGDENAILAELNYNTGDTDFSAQLT
                     EIKKHKPDVIFAPGNYTESALIIKQGKELGIKTPFLGGDTWETPEFIDVGKQAVEGVV
                     FSTFFATETPITETSKVFLDEYRKKYNKEPAAVTALGFDGYLVARDALQRAGSLDKEK
                     VRAAIAATKEFPGATGMITLNEDGDAVKSAVIKTIKDGKFTYMATVEPY"
     gene            5273..6166
                     /locus_tag="PLS88_00810"
     CDS             5273..6166
                     /locus_tag="PLS88_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU05932.1"
                     /translation="MTLSLLFQHIVNALSLGSLYALLAIGYTMVYGILRLINFAHGDI
                     FMVGAYVAFYGITLMSLPWWAALLFSIVFTAFFGIVLERVAYKPLRDSPRISIMISAI
                     GASFLLESLAVLIFGGRPKGMPVPDVLSKVVYFGSVSMITISLIIPLFTFALLIVLLW
                     IVNKTKIGMAMRAVSTDIEAARLMAVDVNKVVSVTFGIGSLLAALGGIMWSFKYPQLN
                     PLMGMMPGLKCFIAAVIGGIGNIAGAVLGGFLLGIIEIMMVALLPTLSGYRDAFAFIL
                     LIIVLLIKPTGLLGKRQAEKV"
     gene            6169..7260
                     /locus_tag="PLS88_00815"
     CDS             6169..7260
                     /locus_tag="PLS88_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU05933.1"
                     /translation="MSKKSSIRGGPISKNMLYTIIFLGACILFIFLADRLFDAFSLRV
                     FNLCAIYIVLALSLNLLNGFTGLFSLGHAGFMAVGAYVSALLTMPAPLKEMNYFLVPI
                     VPWLHHVTIPFLPALIIAGLVSACVGFLIGAPVLRLRDDYLAIATLGFGEIIRVIITN
                     AQSITNGAQGLKGLPRFSTTFAVWTAAGLVVLFMVLLMRSSYGRAMKAVRDDELAAEA
                     MGINVFRVKVTSFTVSSFLAGIGGALLGHMITTIDPKMFTFTLTYNILLIVVLGGVGS
                     ISGSVISAIIVTVMMEALRFLDEPINLIFFKTPAMPGLRMVVFSILLMVMIIYRQKGL
                     MGNKEFSWEMFQNAFVKKSKKGGSHARTP"
     gene            7244..8020
                     /locus_tag="PLS88_00820"
     CDS             7244..8020
                     /locus_tag="PLS88_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU05934.1"
                     /translation="MPEHPELMLSTSHLTMKFGGLTAVSDFNLEIQKNEIVGLIGPNG
                     AGKTTAFNMITGMLSPTEGKVLFKERDITGLKPHSITALGIARTFQNIRLFKEMSVLE
                     NVLVACNLSINTGILAATLHLPGYRARESEARRFSIELLEQVGLAHLANENGTSLPYG
                     QQRRLEIARALATKPKILLLDEPAAGMNPQESFELMDFIRKIRDEFDLTIFLIEHHMQ
                     VVMGVCERMYVLDYGITIAQGDPASIQKNPKVIEAYLGVD"
     gene            8020..8733
                     /locus_tag="PLS88_00825"
     CDS             8020..8733
                     /locus_tag="PLS88_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU05935.1"
                     /translation="MLEIKNLSVYYGGIHALQGIDLEIPDKKIVTLIGANGAGKSTTL
                     RAIAGLVKPSSGEVLLQGSVISGRPTLEIVQKGVALVPEGRRIFSNLSVEENLLLGAY
                     SRTDKKAVIADLEHVYELFPRLKERRAQKGGTLSGGEQQMLAVGRGLMSKPKLLMMDE
                     PSLGLAPLISKMIFEIIKQINSEGTTVLLVEQNAKAALEVADYGYVLETGRISLFGAG
                     HELLQDDRVRKAYLGEAQQ"
     gene            complement(8772..10262)
                     /locus_tag="PLS88_00830"
     CDS             complement(8772..10262)
                     /locus_tag="PLS88_00830"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOU05936.1"
                     /translation="MKFLRRIIPWILVAVIILAALYIPDLGPENYRDFDSLFKNERVR
                     QGIAGYEVAIIKGGEIVFNKAYGFDGQRNLLDTSTPLYIGPASEIFTGTLLAQLVNEK
                     KISLDVPITHFIPELGSLKARTAGGDSLELPLTLRLLAAHRVAFPEKDLPEFDPETMG
                     LEAGLPDADLFLSSHFPTEKYTRSRLSYRIVGAILEKASGMKYSELLESMIAIPLDMR
                     STTAEPSSMLRIAVGAGSFFGLTFPYVEKFPYDAAPSDGIVTTSEDLAKFLRFVVSPK
                     HGQRIPGLSSAQTPMLYQPLYKDGDTGFGWRIIENKEGRSIFQGGSIRGYASRIVIYP
                     ERNSAIAILCPQDGVLISNFLLPKLVSCAEQVLFEGESRRSFPTQRAQLVLGFIFIIY
                     LLNVILQTLMSYSWAKNLLKYRETSISQLYARFVMVRTIVGLVLRILIVLLAPLAIGA
                     LVGWQVTYQEIIAFEPGVSTMLMTALLFGVLRNITRLVMVLRLRHF"
     gene            complement(10417..12570)
                     /locus_tag="PLS88_00835"
     CDS             complement(10417..12570)
                     /locus_tag="PLS88_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOU05937.1"
                     /translation="MNQYSHAFSLTQHFGSRCFSNDVMRERLPRSAFEAILDIQAGKR
                     ELTLDIAEIVASAMKEWASDHGATHYTHWFHPLSGLTAEKQESFVSPQPSGGILMEFS
                     GKDLIKGEPDASSFASGGLRATFEARGYTAWDVTSPAFLKRNADRLILCIPTAFVSYT
                     GLALDKKVPLLRSMEALNCAGLRLLRLLGDSETERIIVYAGPEQEYFLVNAELYRKRP
                     DLKLCGRTVLGSMAAKGQELEDHYYGAIEEKAAAFMNELNSELWAMGISAKTQHNEVA
                     PNQFEIACIYSQANMAADANQLVMETICKIAEHHGLAALLHEKPFEGLNGSGKHVNWS
                     IGTDKGIDLLDPAVVADTSSEEDARFRFMRFVLFSLSVIEAVDRRAGLLRATTATAGN
                     DRRLGGNEAPPVILSIYLGKPLYDLFDSLQSQNNTQKIANALLELGIRSLPKLSRDFS
                     DRNRTSPFAFTGNKFEFRMVGSSQSAATPLTMLNTAVAEALNHISREIEKELTKGMPL
                     LDAARFVAQKSWQEHRRVVFNGNGYSIDWIHEAEQRGLPIIRSAVDAIQELTKPDNVK
                     MLTDLCVLLPEETEARQHIYLERYSKQIKIEAAMMIDLVRRFIIPAASEAARRYSVSC
                     TDIASIGAHFDVQETYAKRIAQLTAKSAEGLDSLEKALDDAIHTENNLERAKLFRDSV
                     APHMDELRETCDELECIVPQDLWPIPTYGDLLFTL"
     gene            complement(12847..14376)
                     /locus_tag="PLS88_00840"
     CDS             complement(12847..14376)
                     /locus_tag="PLS88_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOU05938.1"
                     /translation="MMPSFYSSFSEIEVAASDALMALLRMLFKIGEPEEVLGCFLNSV
                     LPLFQGWAYAACSRTGSGDVLRYIDIGPNEDSWSDENVKIGEEVCIGSVPCGLALEAD
                     EFIQKKLSKGLILEIPIRQRDNIVLVISLLLSEDCASEVKSLASETIQKAAELIEEFL
                     GMRLRLAEKIDRDNGRLINAIDSNLSESPVSWCGIVGNSDVMQEMFSMINQVADTEAT
                     VLLLGESGTGKELVAKAIHQKSSRASKPFVAVNCAALPESVIESELFGHEKGAYTGAF
                     EQRKGRFEQANEGTLFLDEIGELSPALQIKLLRFLQDHRFERVGGNANIEADVRVIAA
                     TNRNLQEAVDQGSFRADLYYRLNVFPINVPPLRERGADILLLSDHFVSQFNRENSKKI
                     QRISTPALDLLMIYHWPGNVRELENCILRAAILSTDGVIHAYHLPPSLQSAASTGTEP
                     KTGLDAEIFRLEKELIIEALKLENGNVAAAARRLMVTERRIRYAIQRHRIDPRRFKTK
                     F"
     regulatory      14512..14695
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            14820..15935
                     /gene="asd"
                     /locus_tag="PLS88_00845"
     CDS             14820..15935
                     /gene="asd"
                     /locus_tag="PLS88_00845"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOU05939.1"
                     /translation="MNNNQSQLQFSPSKKIPVTVLGATGVVGQRFLRRIANHPWFYPQ
                     FLAASDRSAGKKYAEACRWRLSGLSYAGYAEAKVVPCSPELAFSPIVFSALDAGPARE
                     IEPLFAEAGAYVFSNASAFRMEEDIPLLIPELNPEHLELIEMQKAKRRWHGAIVTNPN
                     CTTVMLAVPLAILEEQFGLEAVIVTSMQAISGAGYPGVSALDIEGNVIPFIRNEESKV
                     ESESNKILGRLVKGENVISIVPESFTVSATCTRVPVIDGHTLSISVKLKSKTSLERVV
                     EAFQNYKPKTMVYNLPSAPKHFLRFLEEDDRPQPRLDAEEEEGMCISLGRLRPCPILD
                     FKFISLGHNTERGAAGASILNAEMALSMGMLKEVDNR"
     gene            15947..17275
                     /locus_tag="PLS88_00850"
     CDS             15947..17275
                     /locus_tag="PLS88_00850"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOU05940.1"
                     /translation="MKFGGTSVGSAAHIREVASIVRSREGSRCVVLVSAMAGITNTLF
                     EMAEAARSHDLDRAIRLLGKIEQSHLEASHELDIATPDIIGKIREIIENLRRRIHGID
                     LLGEFSLRVMDEIAAAGERLSSLLVSEYIKCPLLDARKVIRTDNHFGNAHPKLPVIKR
                     NAALYLEPLLESSDIIVTQGYIGSDDADDTTTLGRGGSDYSAGLLALALQADEIEIWT
                     DVEGIMTADPRIVPSARTVDILSYEEAVELASYGAKVLHPATVRPALEAGIPVTIRST
                     FAPYGKFSTISPGDSSGRPCVAVAMRRQVMIISVIQESMTDQAGFLAKLFDIFGRLGV
                     SVDLVSTSEICVSVSLDKRLLFEDLLKNLEELGQVSVADNRAVIAIVGDLLRKTPEVL
                     RTTFIAMDGIPIDLISMGANGINLSLIVQEADAEKAIRGLHTAFFEGGSR"
     gene            17272..17994
                     /locus_tag="PLS88_00855"
     CDS             17272..17994
                     /locus_tag="PLS88_00855"
                     /inference="COORDINATES: protein motif:HMM:NF017024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOU05941.1"
                     /translation="MTIKIGIFGAGKLAHAIVEEVEASNVNEFFVVWMVDKGEMIPDS
                     DIDVAIDASSADAVESHIDWAIRKEIPFVIATTGWDITDLAVRIGEKTGVLVSPNFSF
                     AVAFMQRIASQLARFADWYSDGEIAIFEHHHSAKKDSPSGTAKALARAIVSSSTRYTG
                     WSASGVENCVKDKIPIASLRAGTETGVHEILFDAPHEQFSMTHRARDRRVFAAGALKA
                     AQWIIGRKGLFGMEDVLNDLFS"
     gene            18035..19015
                     /gene="dapA"
                     /locus_tag="PLS88_00860"
     CDS             18035..19015
                     /gene="dapA"
                     /locus_tag="PLS88_00860"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOU05942.1"
                     /translation="MNTIDGLGVAIATPFDDANNIDYEAFERLLDFLVGRDFEASAGE
                     YARRTFGGGAILERFRLFWERESGGAQFVVVLGSTGEGPTVEAEERRELVRRAVERKL
                     GIPVVVGTGSNSTKAAVRLTEEAVDLGADAVLVVVPYYNKPTPAGLIAHFRAVAAAAS
                     SKPVIVYNVPGRTGINLAPSVMQQLWKIENIRAVKESSGNLAQIGEICRALPEGKTVL
                     SGDDGLALPSIAVGAEGLVSVAANVMPRRFRALVDAARAGRRQDAKTLHGELLPFMDA
                     LFVESNPIPVKAALKLCGLAGDGLRLPLVSATKPTKELMESVLAALGCLL"
     gene            19033..19908
                     /locus_tag="PLS88_00865"
     CDS             19033..19908
                     /locus_tag="PLS88_00865"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOU05943.1"
                     /translation="MNKDAETNASFINVGDDITSGAQMDKVAQTLRDVFSEQGHFDKE
                     QWALFLELLENGSIRVVDRLSDGSWKVNTWVKEAILSGFQEGGMVEWSWQSESFFDRP
                     AFSPRHFTLSDGVRIVPGGTAVRRGAFIARGVVIMPPSYINVGAFVDQDTMVDSHVLV
                     GSCAHIGKKVHLSAGAQIGGVLEPPQANPVIIEDEVFIGGMCGIFEGILVKKRAVLAP
                     GVIITKGTRIFDLVRGMDLYSEVPENAVVVPGTRPARGSYAEGLGISLASPCIVKYRD
                     EKTDSAVVLESALRG"
     gene            19920..20729
                     /gene="proC"
                     /locus_tag="PLS88_00870"
     CDS             19920..20729
                     /gene="proC"
                     /locus_tag="PLS88_00870"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOU05944.1"
                     /translation="MQYKLGIIGAGKIGEAILAGSLSKGLLTPSDVILSVRTEQHRKN
                     LEDKYNVTAVLRNREVARQSEIILIAVKPRTVFEVLQEIADVISDESVVVSTVAGVTL
                     DSMQKKLARKVALIRTMPNLGVLVGEGMTALAPARFTEESKIQKVKQLFESVGRALVL
                     DEQYMDAVTGLSGSGPAYVYLIIEALTDGGVKMGLPREVSIELAAQTVLGAAKTVLVT
                     GEHPAKLKDQVTTPAGSTIDGLMELEDGGLRVTLIKAVVKATERANKLLQK"
     gene            complement(20852..22000)
                     /locus_tag="PLS88_00875"
     CDS             complement(20852..22000)
                     /locus_tag="PLS88_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05945.1"
                     /translation="MLIERLAKMYVQWGEPRQSSESEKKRNQQIQAIREFLEVIQKTS
                     ENRKELFAVSQQSRRKATKLSYLLLASDTEPEAIRDSFLRSGLIFKEQHYNKPEVLMW
                     LAGTEACSRGIHPYIVFLIMSAYCGLETANSEFLWLQEKSKLQETKLTEYIVPGDLTD
                     NIEEALSEPVRFQRTIRIAGMPLAASAFAGCSLYYIEQILSLVGPIGSFILTEMIAAA
                     RKRLISDEICTAQQAFLDLYSRQESESSAMDKELMTGMEAFEESSQPDPDLIRTTTDI
                     IMSAEAKALKTTLSSMSDNEIASLLRCLEPIAHERLLGLISQGRQRRILMVIQQTDNV
                     TSERMLRDAQLFAQKLLSSYAPRNLRPGETLKIPELLRNLISSLLGRE"
     gene            complement(21990..22406)
                     /locus_tag="PLS88_00880"
     CDS             complement(21990..22406)
                     /locus_tag="PLS88_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05946.1"
                     /translation="MIYWNADLAAKIRCSSEEMKILPSYIDYLVGSANKIAQGGIQPT
                     SGQFSSEKDDFFRYGLLLVGEGLSGEILEEILAVLLYVSKAEGIDFLKQCIAAEAILS
                     IANGEDEEIMLRKLLPYCGIDVALDTIAQRKSEHAD"
     gene            22758..23522
                     /locus_tag="PLS88_00885"
     CDS             22758..23522
                     /locus_tag="PLS88_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOU05947.1"
                     /translation="MILAEFDTWARSFLEIDQLRQIDDSLNGIQVACDTSKQIQKMAV
                     AVDACAESIRRARNMGADLLFVHHGLFWGKPEPLTGSLRERLKMLFDSDMALYACHLP
                     LDKHPEVGNNAQLANLLGLKERKAFGIYHGISIGWSGVLPKPTKLDQIIAKILPDGSA
                     AKSVIAAGPKEIKTVAIVSGGAPFESLEAITEGIDLYITGEPSHSIYHYLIEGGINFI
                     AAGHYATEVWGVKAIAEKAHAELGLDTIFLDIPTGL"
     gene            23524..24051
                     /gene="lspA"
                     /locus_tag="PLS88_00890"
     CDS             23524..24051
                     /gene="lspA"
                     /locus_tag="PLS88_00890"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOU05948.1"
                     /translation="MKKKVPLAPLLLSWIIIVVDQITKALVIKYIPEGRIFARIFGDF
                     VWIVHTRNTGAAFSIGALSPPPLRFLFFIFLPIAVLITLLFYYFKSENLSPILRYSIG
                     LIAGGGTGNLIDRILRPEGVVDLISVNMYGLLGMERFATFNVADSAITVGEILLILGF
                     IILEFRKPKAQSPQS"
     gene            24100..24375
                     /locus_tag="PLS88_00895"
     CDS             24100..24375
                     /locus_tag="PLS88_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05949.1"
                     /translation="MNKKQANKGKVALFVVGATVANILLMAICFVLFMLLYSVAFSKL
                     LPQEALIWAIGISFLLSLLVSSLIYRRLLKLLRERYHLDDYLGLKAK"
     gene            24435..25793
                     /locus_tag="PLS88_00900"
     CDS             24435..25793
                     /locus_tag="PLS88_00900"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOU05950.1"
                     /translation="MIERLKDLAISIPEIYLPKEGVDYRKWAVIACDQYSSEREYWEN
                     VAQEVGNAPSTLRLIYPECFLEDGDMDQRISAIHRTMNEYLEQGIITSKGEAFVLVER
                     TTPFEEKPRLGLVVAIDLEHYQYGKNSRSLIRPTEDTIEQRLPPRMKIRRGAPLELPH
                     IMILVDDPKKTLIEPLYEQRQYFEKAYDFDLMQNSGHIRGWFVKEERQLCRIAQALED
                     LADPCAFSERYGSNYSADDVLLFAVGDGNHSLATAKAIWEETKNAYKDKPDAGRILSE
                     HPARCALVELVNLHDEGLPFHPIHRVLFNIDAVDFLRKLSAHGAEIRYSSNAKQAFAE
                     VDVPQDSHFFAFVTEKWTGTIRFEEPKARLAVSTIQECIDEYRKAHPEVVIDYIHGSA
                     SIEALGRKKGSLGLYLPPVDKDMLFSVVIQEGVMPRKTFSMGEAPEKRFYIESRLIIP
                     QK"
     gene            25895..26767
                     /locus_tag="PLS88_00905"
     CDS             25895..26767
                     /locus_tag="PLS88_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOU05951.1"
                     /translation="MLILLADAFTPDLPERLAEFGEVTSDMSRIPEAEVLIVRSKTRL
                     DRAMLDKAASCRYIVRGGVGLDTIDAEYARSKGIIVDNTPEASSIAVAELAFAFMIAM
                     PNHLVRADISMKEGKWLKKELERNELYGKTLGLIGIGRIGRELALRAKAFGMSVIAFD
                     KYVLSSDIVKIVSFEEILACSDYISLHIPLIDETRGMIDASAISRMKNGVYLINTCRG
                     QVVIEEDIADALKSGKIAGYATDVYSKEPPEGSPLFEAPNVLMTPHIGASTEENLLRL
                     ADCIVGRLRKYVGR"
     gene            26797..27894
                     /gene="serC"
                     /locus_tag="PLS88_00910"
     CDS             26797..27894
                     /gene="serC"
                     /locus_tag="PLS88_00910"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOU05952.1"
                     /translation="MKRAINFNAGPAALPMEVLLKAQEEMLDWRGTGMSVMEVSHRSS
                     EYEAMHNESQELFRKLAGMGPEWKILFMTGGASTQFFMVPMNYLFRERKATYIVTGHW
                     GKAAVKEAKRFGSIDVISTENEDGSFTRIPKQEELRIDPTSTYVHMTSNNTIYGSQWQ
                     YWPEVGGAPLVCDMSSDIFSRPFPVDKLALIYAGAQKNLGPSGVTLVAIRDDFLYLAQ
                     EQSKLPTMLSYRTFSENNSLYNTPPCFSIYILNLTLKWLLYSIGGLENMQKINEEKAR
                     ILYEAIDSSEGFYRGPVEKESRSQMNVVFRLKTPEMEDAFIKQAKSAGIIGIRGHRST
                     GGIRFSIYNANLVDNIRKAVEFMEEFRLINR"
     gene            28016..30814
                     /locus_tag="PLS88_00915"
     CDS             28016..30814
                     /locus_tag="PLS88_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05953.1"
                     /translation="MAKSRLHRFYNKFISSLSFSAELRKMRRELNAKIDQPEIITSPP
                     PFHPQAANRWFKRRRISIAESYLMVVRDLDSRNSARRLTALKNLADVAFRSSNIDYPL
                     NTARVQSALVKEVVKHRSNKRRQLELLYDFSMSTQGQHQVIRKLCDELNIIELPENGM
                     KIGELGYAWDDHVHDVATSGRKNPTQLVLDAFIKGISSLTVAYGLVSDIDLMEEVLEA
                     GNILGLKVNIGLEFSVLVEGARYHFMAELPHFSSKEELRAFFSDHALDMENFFQGLEI
                     NRESRLDAVRRLLESFNQNTLPKINKDFEDKPEYCLAPLSLEVLLATIPNVNITPLHL
                     AEFMYVRYRPILQKRVWYYKALREKARYMAENTSSLKYGQSGEELREELRREEIEAKY
                     SELKKELNQLSPDTVLSTYFDTPHIISYQSAFDDLESISQMLKKAGCSIKFVQPLEHG
                     LDLAKQALIKRGRCIDAVEIYNIQDCIGRNPEEIEDFARFVNELNKSAAAEGRNLLKP
                     ICGSDATGRNPKIPGMGFIFEDQIIGNLRKRYIKRHMRLPSLISAMIRSSESPVDEAI
                     LKDSAVPRIVCMGKISGSWNRATTGDEERIGLLRAWRYFNPTFKNVIRAITGFTVATA
                     YIGPAYALLWLGITGFRNSIADLISYRGPKLSQWKLKSINFDNVGQSLFWTGFSVPLM
                     GYAKAAFDALWPLAPETFLFNLVKFFVISIVNGFYLAAHNTLRGFDKSVVRANLFRSI
                     LAWPLATVFAPIGNSLSIPSIVQTKIWSDFVAGFIEGGNKYRKVLRQRQKTLEELIPN
                     IINTSGNTQYIAMMDILYLFSQEPRTRTTIKAVLSPYAFFTRKLKENSSLRLNMLLEL
                     NEVMSKENLWTDLVDYIVTNYEEEMADELVELVVDGLPELLKDLSSLIEKYGKDPGLL
                     AKLATKASGTIRRSGQPK"
     gene            complement(30892..31998)
                     /locus_tag="PLS88_00920"
     CDS             complement(30892..31998)
                     /locus_tag="PLS88_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOU05954.1"
                     /translation="MDNELTWWKFDEVDAFMRAGFEAVGVAPDVAAVCSDVLISADKR
                     GVDSHGVGRYKPIYLDRIWAGILNPNTTFDVIKETPTTAVIDGHNGMGLYIAKRSMEL
                     AVEKAEKFGIGMTVCRNSTHYGAAFYYARIAVEHGMIGMTTTNARPSIAPTWGVEPML
                     GTNPMTWGMPSDEDFPFMLDCATSVTQRGKIELYDRLGKDLPDGWVIGQDGKYRHDTH
                     QVLIDLGQDKAALTPLGGLGEDFGGYKGYGYAMVVELLSSALSQTNFMKALLGVDSNG
                     KPKPIELGHSFIAINISAFCDLEDFKHHVGEVSRQLRASRKAPGAQRIWTPGEKEHET
                     WLYRKDKGVPFNPPLKKAFKEVKERCKLDIELPF"
     gene            32249..34255
                     /locus_tag="PLS88_00925"
     CDS             32249..34255
                     /locus_tag="PLS88_00925"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOU05955.1"
                     /translation="MSAHPYTITLDFDDLPPEPIFEPGIRRAPKRDSELTPGQERIAL
                     KNALRYIPPRWHEKLAPEFLAELRQRGRIYGYRFRPHGKICGLPIDSYLGRCIEGRAL
                     RVMIDNNLDFEVALYPYELVTYGETGQVCQNWMQYRLICKYLEVLDENSTLVVESGHP
                     VGIFASHPFAPRAIITNGLMVGMYDNLKDFNIAAAMGVANYGQMTAGGWMYIGPQGIV
                     HGTYNTILNAGRLKLGIPQDKDLAGRLFVSSGLGGMSGAQGKAAMIAQAVSIIAEVDH
                     SRIETRLKQGWVSCEMESCEEAVRLAHVAQEKGEPIAVAYHGNIVDLLEYIDTHDIHV
                     DLLSDQTSCHVPYDGGYCPVGITFEERTRLLAEDRHYFRALVDASLRRHFEVIMHLVK
                     KGTYFFDYGNSFLKAVFDAGVKEISRNGIDEKDGFILPSYVEDIMGPELFDYGYGPFR
                     WVCLSGKKEDLHKTDLAAMECIDPDRRGQDRDNYIWIRDAEKNKLVVGTQARILFQDA
                     FGRMNIALKFNEMVRNGEIGPVMIGRDHHDTGGADSPFRETSNIKDGSNVMADMATQC
                     FAGNAARGMSLVTLHNGGGVGIGKAINGGFGLVLDGSDKVDQVIRMALPWDAMGGVAR
                     RSWARNPHAMEVADQFNCEYGEYGTITMPNLVDEELLERLLGVY"
     gene            complement(34206..36782)
                     /locus_tag="PLS88_00930"
     CDS             complement(34206..36782)
                     /locus_tag="PLS88_00930"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOU05956.1"
                     /translation="MANDIKILENTRVGEGFVPSRYLRPGENEYYLRDEQLRSLGKEP
                     LSFRPLSASELEVLVRNNNTCRDWSLFLVRDPFHPELLRNNIFFGLVRISRLEPATLE
                     HHDFSTTTGIYNSLVIACDIGDNCAISDCAYLSHYIIDDHCILINNAELQVSNHAKFG
                     NGIVMESEEESVRITLDVMNENGGRWIYPFNGMLPADAWLWAKFRDRDALMERFAEMT
                     NTKFDRRLGRYGIIGRDSVLKHNRIIKDVCIGESAYIKGSNKLKNLTINSKTEAPTQI
                     GEGVELVNGILGYGCRVFYGCKAVRFVMCDNSALKYGARLIHSVLGENSTVSCCEILN
                     NLIFPAHEQHHNTSFLIASLIKGQSNLAAGATIGSNHNSRAPDGEIEAGRGFWPGLCT
                     SVKHSSRFASFCLLSKSDYKHELDIRFPFCLVDNDETNGRLMLAPAWWWHSNTYALMR
                     NEKKFKERDKRIDKRIQFIYSPFAPDTMTEILASLAILEYATGKAAIEWLANNEDNRR
                     LFGNKILDLVNELGLHISQKIEAENTKKRSNESAQRPYLENLDIHNPNNTGIRKLGAL
                     ILAGQNDPLPFEVYVKGFENSARQQIVLKPCRARASYREILLWFSVTTLLPTLLTELD
                     KIDDFAEPDEIFERISLCIGLESKELGERKNCQHKAELQEPPEIEWDNMGGFLVQHSK
                     LEDLIERIETGSIQSWDELHCEYQRLSLEYEKDARVFAWAILGYLSLDPEKASAVNVA
                     IARRDASDLEASNLDASDLEERWPKPTLSQLISSLEEAKELSCTIAQRVYATRAKDWT
                     NPFRKTTFRNENEQLAVYGRIEENAAIKAVYHEMEDIRHEIDRLTNRLQASVPKVPHP
                     QD"
     gene            36812..37486
                     /locus_tag="PLS88_00935"
     CDS             36812..37486
                     /locus_tag="PLS88_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05957.1"
                     /translation="MQLDEWGIKRQIRSSPIPLDHFMPFRHGIAMDFVDSARVDTLVT
                     GSEKKVFELFESMVKSTGQERKKSALALANLLGNPGEFSFYIDCTEDQRLIRVFHLLR
                     VFRENMTLLINKTWADGSQNTTQEELLGDLARFIEEYRDFRIVSAFRTFVGIARQIPS
                     LLFGSLGKANDFLEYAFRIDPKFGLFFWYIGEIELQLRNIENIPEHRELFDLEVLIGA
                     FVLSCF"
     gene            complement(37489..38178)
                     /locus_tag="PLS88_00940"
     CDS             complement(37489..38178)
                     /locus_tag="PLS88_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05958.1"
                     /translation="MLFWQISQIPAMAMSLVTAMLPLQTSESSCGYAVAAALINIMRT
                     SVFLAGLEDLEREKHKNPCMPAFENDKTLSRNYGKIPPISLADIKAIIADHGIDSMVF
                     KFSPEALHELVKSINAPLILHVRGQFSHFVIIIDIKSDSKLEAETDVESDTEADTESA
                     GILLFDPSCGLVLLSEFRLKNLVSGYCLLPIRYACKQGEKPVGLEDFETSLSYLKTLL
                     WNVFRTLYCKE"
     gene            complement(38268..40292)
                     /gene="fusA"
                     /locus_tag="PLS88_00945"
     CDS             complement(38268..40292)
                     /gene="fusA"
                     /locus_tag="PLS88_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU05959.1"
                     /translation="MDLEKIRNIGIMAHIDAGKTTTTERMLYYSGKTYKIGEVDNGEA
                     TMDWMDQEQERGITIQSAATTTFWRDHQINIIDTPGHVDFTAEVERSLRVLDGAIAVF
                     CAVGGVEPQSETVWHQADRYRVPRIAYINKMDRVGANFEAVLKDMRTKLGAHPVPLNI
                     PIGAESSFEGVIDLLEMQEIYWSEDGSDMTRSDIAPERQEMAHRWRENLLDAVSSQSD
                     LITKLYLEGVAIPKETLIAEIRKACIAQHFIPVFAGASRRNMGVQFLLDAIVDFLPAP
                     DEVGSIKAYHAKKEQEIEIPCQISGYPLGLVFKVQYDREAGALCYVRMYSGTLHSSSV
                     VYNNSKKKRERITRLLRMHANKSEPIESVSAGDIAVFVGLKLAQTGDTLGTEGYPVLL
                     EKMHFPEPVISVAIEPRTLSDRDKLKEALDILAREDPTFAVHEDEETGQIIIRGMGEL
                     HLDVLVTRVIREFKVQAKIGNPQVTYRESISTKYRHSEKFHKIIAGKENTAGLTIEVE
                     PLPRGTGNQYKKLVRAPDVPQEIFEAIERGIMSAFSSGIQYGYPAVDISVSLIDIEYN
                     ELSSTAFAFEACANMAYDAACRAANPILLEPIMKVDILTPKEFVGEVMSLVSQRGGLI
                     HGTETKIVAEIIHAEAPLATMFGFTTSLRSVSQGRASFSMEFSHFEPKRD"
     gene            40575..42725
                     /locus_tag="PLS88_00950"
     CDS             40575..42725
                     /locus_tag="PLS88_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOU05960.1"
                     /translation="MKKVELLAPAGSREALDAAIGEGADAVYLGLRSFNARMRTTNFA
                     FNQFEAAVYACHYRSRRIYVTVNTVFEERESDRIYQLLEYLATVGPDGIIVQDAGVAK
                     IAHDYFPSLPLHASTQMNIASAAGCNQLSRAGFKRAVLARELSLEEIRQIRQYTSLEL
                     EVFVHGALCVSASGLCLFSSYLGGKSANRGQCTQACRRLYESEKGKGYYFSPDDLELI
                     EYLPDLITAGADAFKIEGRMKSAEYVGTVVSAYRFMLDNYEKDRERAILKAKAILQSD
                     FARRKTNFFISGKSEDYIHPEQSGGTGIALGKIRDARTIDGKRWALLNSYEGLAERDS
                     VRIHRKDDSGRITAKIQAIRYEVYGMLLLLDGDWRQNDELYLIQTASMTRNYKPVLPK
                     NLDKFHKFPSNHAAPRPALLRLDGRKLEALFEPGNYVLAGKVAHLHAALTFRPKKAMI
                     LFNKLNAETMRREEESLPFKRDRLILWLDPYLAESDSTWLTAELDYWLEKGVSLVIVN
                     NQAHFSMLRGKKVISIAGPWLYTFNQWALAYYIEQGVQAFIPPYEISRQDLYRLTEYL
                     PAQFFAPIVFAYPDLFRIRADLSKKYGQANFIDRENNTFRLIGKRDYSVVVPERPFSI
                     TDLVPNLKKQGFRRFIVDLSNAEPSKGLYRDIARAVDQGSSLLNTTRFNWKEGFWSEE
                     TIRRDASKDDSKDASINKKPKSPPYAQRRQSRTR"
     gene            42933..43715
                     /locus_tag="PLS88_00955"
     CDS             42933..43715
                     /locus_tag="PLS88_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOU05961.1"
                     /translation="MSESAGKAFDLLFYLAKTGEAVSLARLSKETGMNKATALRYLNV
                     LESKGVVERCKMGWTLGLSLFELGSKVPVRQLVADKVRPIIERLARETGESVNLAYLA
                     DETAIFLDRAEANRSLRMRSMPGDRLPLYCTGVGKAILSQLSEERIYAILGPGPLEKF
                     TESTYTDPEDIINEARRARELGYGIDSEEFEVGLTCYAMPLRIPSSDFVGAISISGPT
                     ARMKNPEIRDRFIYLLKQAIKDATEVLSPHCYLDNISKKAKS"
     gene            complement(43791..44207)
                     /locus_tag="PLS88_00960"
     CDS             complement(43791..44207)
                     /locus_tag="PLS88_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU05962.1"
                     /translation="MNHLNSILVEGNLVRDPNFRSTPSGNQVCDFTVATNRNYKIADQ
                     KYENEVSYFDVEAWARLGSACAQNLKKGRGVRVVGRLKQDRWTDTEGKSHSRVKIVAE
                     HVEFKPMSKATQAEAPNSETPFIEDSKRGEIETPVF"
     gene            44334..45731
                     /gene="purB"
                     /locus_tag="PLS88_00965"
     CDS             44334..45731
                     /gene="purB"
                     /locus_tag="PLS88_00965"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOU05963.1"
                     /translation="MESFDRFQSPFTWRYGSDKMREIWSEHNKRKLWRRIWIALAKAE
                     SSYGLVTPSQIAELESHADDIDLEKAGKIEDSIHHDLMAELQVFASQCPSAGGVLHLG
                     ATSMDIEDNAEVMRIRESLSLLQRKLTKLLLKLAEKIELYASLPVMAFTHLQPAEPTT
                     MGYRYACYSQDLVDVYKRLSYTIADIRGKGFKGAVGTSASYVELLGLDKLDDFERALS
                     ENLGLQFFDVATQTYTRVQDYRIIALLAEMGAIFHKMAFDFRILQMPMLGEIREPFGE
                     HQIGSSAMPFKRNPIECEKVDSLARLLLSYSNVAWDNAALSILERTLDDSANRRIVLP
                     EAFLAADELITTMQHIIDGQIIDKEAIQLTLDTYAPFSATERILMALGKRGADRQISH
                     GRLRDMAMLAWKAVKEGKTNPLKSLIKSDEFFASFLSSDTIEKLFDVDIYIGAAEQRA
                     LAVAERIRRFAMDEC"
     gene            45781..46434
                     /locus_tag="PLS88_00970"
     CDS             45781..46434
                     /locus_tag="PLS88_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOU05964.1"
                     /translation="MLDFISKGGPILWVIMGLSVVALAIVIERLLYLRRISIDEEKLF
                     LRIKTSLMEGHFSEALAICDQNISPFSTLFKVGIENRKQPEYLQRELLKDAASLESVN
                     LERGLTTLGTISNISTLLGLLGTVTGTMRAFGVLGKFGAVSDPAALASGVAEALITTV
                     GGLVVAIPVIMIYNYFVSRVNLIMTRLETQVNNLVSFISSYGGKEPDSAEEKPSKEA"
     gene            46434..46850
                     /locus_tag="PLS88_00975"
     CDS             46434..46850
                     /locus_tag="PLS88_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU05965.1"
                     /translation="MARNHKLSPKINFDLTPLIDIILQLVIFFMITTTFRTAPGINLQ
                     LPSSKTAQTISTPELRVVVMSSDEIYVDKTKTNLTSLESVLGKRVSGSDAKQIRAILE
                     GRAGAEYQLIISVLDALRMNGIENVGLITRKEKVVR"
     gene            46847..47875
                     /locus_tag="PLS88_00980"
     CDS             46847..47875
                     /locus_tag="PLS88_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOU05966.1"
                     /translation="MSEALTYAEREQRKRTILSYIIAIVMYAILFGAGLLFNILKPES
                     IDFSNKSLIVNIQGPVTNDIGRGSPIEKEEGLVAEKPAPPPASVPEPKAQTSIKPKTA
                     EPVATSPAAKPEPTPTPITPETTAIQEPEKPWIPGERGPGSRISSSESSVLSPGEGQV
                     PWGTGEAVRITKAEKGNTVETTLGGSSESVGQSLYVPIYLSMPLPEVVPAALFNAIPP
                     EIVPPNTLIASAESRKRAFLNYYSWNGKEYRLTNPVPLEVRDKLWEMLEDAGYNVEKA
                     DYKAGRSLNPIVIGFSITKDRQLRGLEILQSSGDPEIDAAVLYGFKRASFWNKSGDTI
                     QGRFVYRF"
     gene            47955..51131
                     /locus_tag="PLS88_00985"
     CDS             47955..51131
                     /locus_tag="PLS88_00985"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU05967.1"
                     /translation="MIFNFSSLIAALSFCIYVIFIVFGLSGRKERVSVSFLIYMVMMA
                     LWSFGAFMAYGNIGLISLTNSIKIKLVGLLGAPFAIFATLLYFSKSEKKLYRNLLYIG
                     YIIYAYLLYLNFSSSIVADVWFEGLDYNYRLGSDALIAYFFSYAYLILTIFFLLREQK
                     ANQNKFLKRALQLMLIGVVVLMIGVLAYLYEPIGRYPVDMIAAMINSSIIFYSIYKYR
                     LVQYSAAVFDLILGALVAAFSSIIYMMFFVFIFRLNRFVPNSTLVPLALCLGLCSAFL
                     FGPLHTAIQTLFERISGRKSFLYYRELRDFSTKLTSIVSLEELSRLIVDNVISALKLE
                     WAFVLINEYRSQNFRVIASHGLDFIQEQGSQNPTNQVIVPKSSPFIQAYQKKAAQEKQ
                     AYSQWNIGIMLIKGQKHQTVLASLVLPLRFKERLNGFIILGPRLEKDYYNKYELDILQ
                     FLVDQASVAMENAITYERLRQHQKRLQDANKQLINQRKFETFFDGIATPISIQDINYN
                     IVSANYAAQRYFEKTQEELIGSKCYKAYFNRDRPCAECLAQDCLHAMLPFNTEKQDSR
                     TQLTFSLNFYPIPTREASMGAFIELFQDITKQKSQHEELLSEEKLAAIGSLVSTIAHE
                     INNPIGGILGTTDLILEETPEGSPIRGYTEDIARYSQNALDFIKSLMVYSKKTRTTLE
                     MINIINVLENSLKMAMRSIDFGSVVVRKSYDSVKEVLANPTELQQVFLNLIINSVQAM
                     RSDGVLTLSCRQERDDVLVSVQDTGIGMRSENFDRIVNNFLTVEETSSATGMGLSFAN
                     HIVTKYGGRILLDSQLGKGTTFTVILPSAIQDKDRIWFIHASEPRQFDDSYYLQLKVL
                     VGEKGYQEETIHRKCDDSAFHIIAYKGFQPIGTVSVHLSEANGHIPIEENFQISPFLR
                     GVPYAEIDRLAVTKDERLGLTPFEIMVLSYLYARGWGAKQIFIDVFADEASQIQMFKK
                     VGFEIIGSYSNPLPCTVMMMNHASIYEQKTPNNQLFIKRTISRLIPKIDFSAEELSLV
                     MKAIDEINAIFPRQDTVDTKEISSYNLS"
     gene            51133..52194
                     /locus_tag="PLS88_00990"
     CDS             51133..52194
                     /locus_tag="PLS88_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOU05968.1"
                     /translation="MKPEDLAEGLNIIFAHSPVFSDQSLIIDVVANPKAGGFSRIHNS
                     NKRMRELKEMVKRSSDLPLRKAPFSLKMHLTERCGHAATIVQRILEHSPSNGKDTYHL
                     IITAGGDGTSLETAEQLTLLPESEKDRFGIVRLPFGTGNDGSEGRDLFVALERFLNPA
                     ILERRPALKITPSYEGGALPRYSFNIASVGLDAYVAEMTNRLKRHFPGDSYKFWVDVG
                     TMFYDRVYKVVNMGLRAWNRGRLVLDSELPRLLVAMGASGNRYYGSHKSILPNEHNCI
                     AISQTSLFRKLILKGSIEHGKHDHIRELTHFTADKLQIDYHESIPLQCDGETTILAKC
                     DFPLEIEKLQNAYNVLVPV"
     gene            complement(52170..52739)
                     /gene="thpR"
                     /locus_tag="PLS88_00995"
     CDS             complement(52170..52739)
                     /gene="thpR"
                     /locus_tag="PLS88_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOU05969.1"
                     /translation="MRLFAALPFPVATQNQIGAYCASLEEAFRPARPSWVPPQNLHLT
                     LHFFGELEAEDAEKLKILLSDLANLCPPLRITTEDLSMLPSMNRPRVLYLATHIEPFE
                     PLTILINRIREIAIQLGAETDARPWKAHLTLARLKLPIAPELSSLPRPPKLSLSIDSF
                     ELLRSTLSPSGAVYSRISRFALYTGTSTL"
     gene            complement(52746..53477)
                     /locus_tag="PLS88_01000"
     CDS             complement(52746..53477)
                     /locus_tag="PLS88_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HOU05970.1"
                     /translation="MKNARTTLSKLSEIALRRYSMIQPGDRILIAVSGGKDSCCLAWD
                     LAKKRQWWETPFEIAACHIVTDLEDASFSQTQDDAWLALRMTEWGIPYTRLEIPVVGR
                     LKPGRSMNCYWCTTQRRSELMKYAMAHGFNKLALGHHLDDILETLLMNMLKKGEFATM
                     PPVMPYRKYPITVIRPLALCEERQILACADELGLSAYTCTCSFNLQGERKKARELLEH
                     LTEGSSHKKRNIFESMSRIRKDYLP"
     gene            complement(53584..54207)
                     /gene="udk"
                     /locus_tag="PLS88_01005"
                     /pseudo
     CDS             complement(53584..54207)
                     /gene="udk"
                     /locus_tag="PLS88_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537791.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0046108 - uridine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
     gene            complement(54250..55134)
                     /gene="ispH"
                     /locus_tag="PLS88_01010"
     CDS             complement(54250..55134)
                     /gene="ispH"
                     /locus_tag="PLS88_01010"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOU05971.1"
                     /translation="MQIVRARILGMCMGVRRADELAHRAAEKAAQTGRPVYSYGPLIH
                     NPQAVADLESLGVRVLEPSAIESELDELPNLKDAIVIIRAHGASLAALSRLHVLGARV
                     VDATCPRVIKSQGLARHYERLGWQIVLIGDPRHGEISGILGHTLNALVVDSPETALPM
                     ARALRDKPCALIAQTTIRQEDYDAIIDIFQSYVKKMIAEKTICPATRERQQALAELST
                     QVDAVLVVGGKNSENTKRLYKTAIGFGLPSWHIETASELTPEMMRYNRIGITAGASTP
                     DFIVDEIQEMLVRMAQSE"
     gene            55247..56191
                     /gene="arcC"
                     /locus_tag="PLS88_01015"
     CDS             55247..56191
                     /gene="arcC"
                     /locus_tag="PLS88_01015"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOU05972.1"
                     /translation="MATRKTIVVALGGNAIIEEGSEGTIEQQFENTRKSMKAIVGMIA
                     EGHKVVLTHGNGPQAGVHLIRNEAASAQVPPSPLNVIVADTQGSMGYMIAQSLANALR
                     TEGIDKDVVTVITQVVVDPNDPSMLNPTKFVGPFYKAEEVEKLRARGWIIKEDPMRGY
                     RRVVPSPKPLDVIEKDTIKDLIDDGKIVIAVGGGGIPVKRDADGILSGVDAVIDKDRA
                     SSLLASLIDADELIILTGVDKVAINFRKPDQRVFDHLSLKECEQYMAEGHFPKGSMGP
                     KIEAACDFIRRGGAKVIITSMGNATAAVNGKAGTVITA"
     gene            56259..56687
                     /gene="yajC"
                     /locus_tag="PLS88_01020"
     CDS             56259..56687
                     /gene="yajC"
                     /locus_tag="PLS88_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOU05973.1"
                     /translation="MNGIQNGLFLLQQTTASNPTGSLISTIITFGLVFVIFYFLIIRP
                     QNKKQKQMQQMIAGVKKGDKVVTIGGIHGVVASVKDSTVVLKVDDGTRIEFSKSAIAS
                     ISPVKAEEKAEDKPDEKAEEKPEDSAESTRKTDKEKTEDK"
     gene            56781..>56989
                     /locus_tag="PLS88_01025"
     CDS             56781..>56989
                     /locus_tag="PLS88_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOU05974.1"
                     /translation="MSKLRRLMIVIAVLAVAFMFLFPSIQWYFLTPKEDQAIAVGSRE
                     QIREYSRRMTYLIISDLKERAAAGDT"
BASE COUNT        15115 a        12615 c        13737 g        15522 t
ORIGIN      
        1 gtgcatatag ccagtatatt ctaagtctca ctcatgccac attacgccgc tcaggccgct
       61 gcaccgacag ccaaaagcgc agaaaaacta tactctatcg atcattagtc tttgaaaagg
      121 ctgccctcga acgctccttc tcagagcttc tagatcagtt atatagggaa cgctgtagtg
      181 cttactcttc accgctaatc ctcatcacag atgaaaagtg tgaatatgct cgagcgctca
      241 aactacaccc tagtcgcttc tctatacatc atctcaccgt aaattccaaa gtcccacgta
      301 ccttccagaa tcctctcttt gcctcaaact atctcgacag agagcttaga aaggatcttg
      361 ctgatcatcg tcgcgaaaca gtttgttttc cgagaaatgt tgcgaatatg ctgaatcgcc
      421 ttgtcgtata cttaggctgg cataattatg aaaaacctta caggattgga agggatatcg
      481 ttctaacgca tgcggaagta gctggtattg aaagagaagc gatttgtaag gctagagaga
      541 agcagttcca agaacgcgct tttctaagca gagcaggatt atcgcttctt gaaaaaaagc
      601 tatggcttag aagtttccca actcccctta agagaaaagc ggagtatgta cctgcttatg
      661 cttatgcatg agatactcta ctaccaaatc tctaaagcca tttacacaag gttcgtagca
      721 gtattgattt ctttacgaag ccacttgaca tatttatgca atcggagtaa aaaatattca
      781 tggcgcgagc aatattctgc aataatggca actccctcaa taataataat cgcatacttg
      841 gagcggggcc ggagccgaat atataggcgc attgaagcgc gaaaactgaa gccggtcccg
      901 ttttcggggc tggctttttt ttacgctgca tattgcgcta gtttttccga aactggaggt
      961 gcaggggatg gcggagagtt tctgtttccc cggtggtaat gggatttcga gtattaaacc
     1021 agtatgccgc aatgaacgtc gttcctgcgg ttctggcact ctcgaagaat ctctccacag
     1081 ggagaaaaat catgaaatca tttgcttcag tagatgccac cagcacctca attggtggcg
     1141 tattggaacg ttttcaagct tctcttggca atagagaact ctcatttagt gagtttttat
     1201 ccgatgctta tgtacaaaag tggaagggag agcaacttgc ccatgcatta aaagtgagaa
     1261 cagctcttgt gttgggactt caggagtttc tagtttccag agggattctc aacatagata
     1321 gggtatcgct ctcgcccgta acagatcctc tatgccaaga tgtcgagcat gtccccataa
     1381 ttctctatca agatacgcct tataggacta cacattctat gatctattcc aagatgcttg
     1441 cttgtctaaa tccatatata gaaggaatat acgtagactc gccgaatatt cgtctcgaat
     1501 tgccctcgct gaattcaagg cataaatacc ttattgattt ttctcaaatg gatatcgaat
     1561 tcaaacgagc acacaagctt agcgaggacg actatttcga taggccaaaa gagacggctg
     1621 agattttatc taaagaacta gatcgggcgc tcgatttttt cgaggatttg atcatttata
     1681 gtacaaaaaa aattttagtg ctggctgcgg agagtttgga agcactagga gtcaagctca
     1741 ctgttccatc gaagccattt ccaagattct atagcgatga aatcgaggta cagaatggat
     1801 gcagccttga agagcagctt gggcagaaag ccaagacaca atttttctgg gtactagggc
     1861 ttttgcgaga gaactacgat cttgtgtatc cctatctatc tcgagatgga agaaggcctt
     1921 cgaagaagga gatctctcca aggcaagtct tcaattatga cttatgtgca gcgccccttt
     1981 atagagatgg gagccttggg catgcatatg aaatcctctc tggcggcttg cgggaatggg
     2041 tctacgaagc gattgttgag cgccttctag ataatgccgt tctgagggag tcaccacgct
     2101 tcgatcgata cggaaatctc gaaaatatgg ccgcgttaga tggctatggc ccatttctga
     2161 gcattgctca catgagagat tcgcaagacc agagattctt tccgcaggtt tgcggtggag
     2221 ggcttggcat cgagcgcaca ttattcgcgc ttctccaggg gccattcatt aaggatatag
     2281 acgaggttac ttgttttggc aaaaatccgg ataaaacatt gccttttcta ttctagggaa
     2341 ggcttgttgg gtacagagaa aacttgtagg gctaattcca gcaaatggct ctagcaaaaa
     2401 gttgcgcctt cggtttttta tgtcgcttta aaggtcaatt attcgataac ctaataattc
     2461 catgggcggc tctcatcccc gaagcccaag ccgaggtaat ccctcggctt gtccccgcac
     2521 catcgcctat aaaatagatt cccggcaaca cttggaagtt ctcatcgata aaaactgggc
     2581 ggttcgcata cagtttgatc tctggatagt acatgatcgt cgatggatgc ataacgccag
     2641 gcacaattgt atctagcaat ttcatcgcgt tccaaatcgc gcgcattgtc tttgccggaa
     2701 tgcagaggct tatatctcct ggcgtgcagg atggaagaga agggttgaag ttatagagat
     2761 cgccagtaaa agagttggca aatgatcgct ttccaagcct aaaatcgcct atgcgttgca
     2821 ttatcggtct tccgcctcca agcaatgcag ccaagtgtcc taacatctgc gcataatctt
     2881 gtccggaagc tagtggctgc gtcaatgtta tagtttttag aattgcaaag tttacaaggc
     2941 catttggtgg tcgctcatga gaccacgcat ggccattgat agagtaccaa taaccttgtt
     3001 tttcatcgcc atagcgttcc tgcaccacat aggctgctcg agaattggta cagaaagtac
     3061 gcgttttctt ggggaaaagg aatttaggat cataataatc gctcactatt gggtagtgtt
     3121 cttctgttgt ttcgatccgg acgcctatat cgaccacatt gtcgcgatat ttgattccaa
     3181 gttcttccat aagcctttgg agaaatgcaa aaccaccacg gccaggtgct actattaaag
     3241 agtcatattg cagctcgcgt ttatctgtta ttagaaccct ggatcgaggc tcgactctaa
     3301 gcatctcctc tccaaagctg aaagctgcac cgagctcttt gagccttgct gtgagatcct
     3361 tgattagctt aaaaccacca tcggtgccca ggtgcgactg gcgcacatcg agcaattgaa
     3421 cccctatttt ctctgcccgt ttcctataga tttctatgtt gcgcttttcc atgaaatgtg
     3481 gctcgagaaa ggcttcgacg cgctcaaggt aatgtttgcc agtagcctca tcccaacact
     3541 ccaggggaaa acctataggc caagtaaaat tcatcttgca atcgtttcta agcccgcccg
     3601 aggatacccg ttgtttgtcg attaggagaa tattgaacga tggtctcgat tctagaagct
     3661 cgaatgcggc accaagtcca gctgggcctg tacccacaat cacgacatca tagctttcca
     3721 tgcgtttttc tcctcggcct attaaatcat ttcgctcatt tctgcgcaat gcgaagacaa
     3781 gaacagattg gatgcatcga gattttgaag cgatatgtgc gtggttctaa ataccattta
     3841 ttgcttcaat attattcatt aaatctccta aatagtgtaa aatatatttg acagaacgcc
     3901 tccggcgttc taccattgat aatgtcccat cagtatttga aatggacata caagaaggag
     3961 gtaatcatga agagaattgt gaggattggg ctattaacca ttggagtcgc tctgctcatc
     4021 gtagcttgtg ggccaggtgc tcctaaaact gtaaagtttg gcgtgttcga gccactgact
     4081 ggcgcaaatg ctggtggcgg cgcactcgaa gtcgaaggta tcaagctcgc aaatgagctc
     4141 tatccaactg taaagattgg cgacaaagaa tataagatcg agcttgtaat tgctgataat
     4201 aagtccgaca aagtcgaggc ggccaatgct gcacagaggc tagtcgacaa ggacaaagtc
     4261 aatattgtgc ttggatcttg gggttcttcg ctctccatgg ctgctggtcc catcgtcaag
     4321 aatgccaaag tctcggcaat aggtctatct tgcaccaacc cattggttac aaagggcaat
     4381 gaatactact tccgcgtctg ctttatcgat ccattccagg gcacggtcat ggctaattat
     4441 gcgtacaata ctttgggtgc caagaaagcc gtaataattc gcgaagtttc gaacgattac
     4501 tctgttggtc ttgcaaaatt ctttgaagat gcattcaaac agttgacagg cgatgagaat
     4561 gcaatccttg ctgaattgaa ctacaacacg ggcgacaccg atttttccgc ccaacttacc
     4621 gagatcaaga agcataagcc cgatgttatc tttgcaccag gcaactacac tgaaagcgct
     4681 cttatcatta agcaaggcaa ggagctcggt ataaagaccc cattcttggg cggagataca
     4741 tgggagactc cagaatttat cgatgtcggt aaacaagctg tcgaaggcgt cgtgttctct
     4801 acattctttg caaccgagac gcctatcacg gaaacatcca aagtattcct cgatgagtat
     4861 cgcaagaaat acaataaaga gcctgcggca gtcaccgcac ttggcttcga tggctatttg
     4921 gtagcacgcg atgcactcca gcgcgctggc agcctcgaca aggaaaaggt tcgcgcagca
     4981 atagcagcta caaaggaatt cccgggcgca accggaatga ttactctaaa cgaggatggc
     5041 gatgccgtga agagcgcggt gatcaagacc atcaaagatg gcaaatttac ctatatggct
     5101 acggtcgagc catactgata gccgatagcc taggatttta actggaggtg ggatgatggg
     5161 cgcatagaag cgtccaccat cccgatatat cataagtcaa ttgaaggagc ggaggttcat
     5221 cgaagaataa ggtcgagtgc atccgctcct tttcttatat ttacgaggaa gcatgacact
     5281 ttcattatta tttcagcata tcgtcaatgc gctctcgctc ggaagcctct atgcacttct
     5341 agccattggc tacacaatgg tgtatggaat attgaggctc ataaactttg cacatggcga
     5401 tattttcatg gttggtgcct atgttgcttt ctatgggatc acgcttatgt ctttgccatg
     5461 gtgggcagcc ttgctttttt cgattgtctt cacagcgttt ttcggtattg tcctcgagcg
     5521 agtagcttat aagccgctta gggattcacc gcgaatctct ataatgataa gcgcaatagg
     5581 agcatctttt cttctggaga gtcttgctgt tttaattttt ggaggaagac caaagggaat
     5641 gcccgttccc gatgtgctct cgaaggtcgt ctatttcggc tcggtttcga tgataactat
     5701 cagcctgata attccccttt tcacttttgc gcttctgatt gttctccttt ggattgtcaa
     5761 taagaccaag atcggcatgg caatgcgcgc tgtttccaca gacatcgagg cagcccgctt
     5821 aatggcagtc gatgtcaata aagtcgtatc tgttactttt ggcattggct ctctgcttgc
     5881 ggctttaggt ggcattatgt ggtcattcaa atatcctcag ctgaatccgc ttatgggaat
     5941 gatgccaggc ctaaaatgct tcattgccgc agtcataggt ggtataggca acattgcagg
     6001 cgcagtgctg ggtggattct tgcttggcat aatcgaaata atgatggttg cattactacc
     6061 gaccctttct ggttataggg atgcttttgc atttatcttg ctcatcatcg tcctattgat
     6121 aaaacccaca ggactcttag gcaagcgcca ggctgagaag gtgtgaccat gagtaaaaaa
     6181 agttccatta gaggcggacc gatttctaag aacatgctct atacaattat ttttcttgga
     6241 gcatgcattc tcttcatctt ccttgccgat agattattcg atgcattttc gctgcgcgta
     6301 ttcaatctat gtgcgatcta cattgtgcta gctctttctc ttaatctgct aaatggcttt
     6361 acgggtctct tttctcttgg gcatgctgga ttcatggccg tgggagccta tgtgagcgcg
     6421 cttctcacta tgcctgcgcc tctcaaggag atgaattatt tccttgtgcc aatcgttcca
     6481 tggctccacc atgttacaat tccatttctg cccgcgctta taatagcggg gcttgtgtcg
     6541 gcttgcgttg gcttccttat tggtgctcct gtcctgcgcc ttagagacga ttatctcgca
     6601 atcgcaacgc ttggttttgg cgagatcatc agagtaatca ttacaaatgc ccaatcgata
     6661 actaacggag ctcaggggct taagggtctg cctcgctttt ctaccacgtt tgcggtatgg
     6721 acagcagcgg gtcttgtagt gctttttatg gttcttctta tgcgctcgag ctatgggaga
     6781 gcaatgaagg cggtacgaga tgatgagctt gctgcagaag cgatgggaat caatgtattc
     6841 cgggtaaagg tcacctcatt taccgtgtcg agttttctgg ctggtattgg tggagctctc
     6901 cttggccata tgatcaccac cattgaccca aaaatgttca ctttcacgct tacctacaat
     6961 attttgctga tagttgtgct tggaggagtc ggctctatct caggttcagt gatatcggca
     7021 attatagtta cagtaatgat ggaggcgctg cgtttcctcg atgaaccgat aaacctcatc
     7081 tttttcaaga cgccggccat gccaggtctt cgtatggtcg tattctcgat tctcctaatg
     7141 gtgatgatta tctatcgtca gaaggggctc atgggcaata aggaattcag ttgggagatg
     7201 ttccagaacg cattcgtcaa gaaaagcaag aagggaggca gccatgccag aacaccctga
     7261 gcttatgctg agcacctctc atctcacaat gaaatttggt ggccttactg ccgtaagcga
     7321 ttttaatctg gaaatccaaa agaacgagat cgtcggactt ataggcccaa acggcgctgg
     7381 aaaaacgacg gctttcaata tgataacagg aatgctgagt ccgaccgaag gaaaagtcct
     7441 tttcaaagaa agagatatta ctgggcttaa acctcacagc attactgctc ttggtatagc
     7501 gcggactttt cagaatatca gactatttaa ggaaatgtcc gtgctcgaga atgtactagt
     7561 tgcctgcaat ctgagcataa atactggaat actagctgca accttgcacc ttccaggata
     7621 tagagcccgc gagtccgaag ctcggcgatt ttccattgag ttgctagaac aagtaggcct
     7681 agcacattta gcaaatgaaa atggtacaag tcttccgtat gggcagcaaa ggcggctcga
     7741 aattgcgcga gcgctggcta caaagccgaa gattcttctt ctcgatgagc cggctgcagg
     7801 tatgaatcca caggaatcgt tcgagctcat ggattttatc cgcaagattc gagacgaatt
     7861 cgatctcact atcttcttga ttgagcacca tatgcaggtg gtcatgggcg tgtgtgaacg
     7921 catgtatgtg ctggattatg gcatcactat tgctcaaggc gatccagctt ccattcagaa
     7981 gaatccgaag gtcatcgaag cctatctagg agtggactga tgctggaaat caagaatctt
     8041 tcagtttatt acggaggtat tcatgccctc cagggaatag atctcgaaat tccggataaa
     8101 aagatcgtta cgcttatagg ggccaatggc gctgggaaaa gcaccacgct acgtgccatt
     8161 gcggggctcg taaagccgag ctctggcgag gtgcttctcc agggatcggt catatccgga
     8221 aggccgaccc tggaaatcgt gcagaaaggc gtagcactgg taccggaagg caggcgaata
     8281 ttctcgaatc tttcggtaga agagaatttg cttcttggtg cctattctag aacggacaag
     8341 aaggccgtga ttgcagatct tgagcatgta tatgagcttt tcccaagact taaagaaaga
     8401 agggcgcaga agggcggaac gctctcgggt ggagaacagc agatgcttgc agtaggtcga
     8461 ggacttatgt cgaagcccaa gctgctcatg atggatgaac cttctctagg tcttgctcca
     8521 cttatatcga agatgatttt cgaaataatc aagcagataa attcagaagg aacgacggta
     8581 ttgcttgtag agcagaatgc aaaggcggct cttgaagtag ctgactatgg ttatgtctta
     8641 gagactggtc gcatctcgct ttttggtgcg gggcatgagc tactccaaga tgaccgtgtg
     8701 agaaaggcat atctgggcga agcgcaacag tagagagcaa cagcagagcc tatttgggcg
     8761 acgaattaat tttagaagtg cctcagcctt aagaccatta caagcctggt gatattgcgc
     8821 aaaacgccaa acaatagagc tgtcataagc atagttgata ccccaggctc gaaggctatt
     8881 atttcctggt aagttacctg ccatcccacg agcgctccaa tggcgagggg agccaagagt
     8941 actatgagaa tgcgcagcac caagccaaca atcgttctaa ccataacaaa gcgggcataa
     9001 agctgggata tgctagtctc gcggtactta agtaggttct tagcccagga ataagacata
     9061 agtgtttgca agattacgtt tagcaagtaa ataataaaga tgaaaccaag cacgagttga
     9121 gcacgctgtg tggggaagga tcgacgactc tctccttcga acaatacttg ttctgcacag
     9181 gagaccagct ttggcagtag aaagtttgag atgagcacac catcctgtgg acagagaata
     9241 gctattgcgg agttgcgctc aggatagatg acgatacgcg atgcatagcc tcgaatcgag
     9301 cctccctgga aaatagagcg accttctttg ttctcgatga tacgccatcc aaaacccgta
     9361 tctccatcct tgtagagggg ttgataaagc atgggtgtct gtgctgaaga gagcccaggg
     9421 attctctggc catgttttgg cgatacaacg aatctcaaaa attttgcaag atcttcgctt
     9481 gtggttacta tgccatcgga aggcgctgca tcatatggga atttttctac atagggaaat
     9541 gtaaggccaa aaaacgagcc agcacccacc gcgatacgca gcatggaaga tggctcggcg
     9601 gtagtagatc gcatgtcaag cggaatagcg atcatcgatt caaggagctc cgaatacttc
     9661 atacccgagg ccttttccag aattgcacct acgattcggt aagaaaggcg actccgtgta
     9721 tatttttctg taggaaaatg ggaacttaaa aatagatctg cgtctggtag acctgcctcg
     9781 agacccattg tttccggatc gaattctgga aggtctttct ctggaaacgc aacacgatgc
     9841 gccgcaagaa ggcgtagagt aagtggcagc tctagagaat caccaccagc tgttcttgct
     9901 ttgagcgaac cgagttctgg aataaagtga gtaattggca catcgagaga tatttttttt
     9961 tcgtttacga gctgtgcaag aagcgtcccc gtaaagatct ccgacgctgg cccgatatat
    10021 aagggggtag aggtatctag aagatttcgc tgtccatcaa agccataggc tttattaaag
    10081 acgatttctc cacccttgat tattgctacc tcataaccag caattccctg gcgtactcgc
    10141 tcgtttttga aaagcgaatc gaaatctcta taattctctg gtcctaggtc tgggatgtag
    10201 agagcggcaa ggatgattac agcgacaaga atccatggaa ttattcggcg cagaaacttc
    10261 atcagtcact atcgtagcag gagcgggcta gtagagcaac atggcctcag tttcctagat
    10321 tatagcgctt caagctggag tgtctaaact atagtgctcc agagtgctcc agaccttagc
    10381 gctccaaacc tcttacctga tgagtggact cttaactcag agagtaaaga gtagatcgcc
    10441 ataggtgggg atcggccata aatcttgagg cacaatgcac tctagttcgt cacaagtttc
    10501 tctgagctca tccatatgag gggcaactga gtctcggaat agctttgctc gttcaagatt
    10561 attttctgtg tgtatagcat cgtcgagggc tttctcgagg gaatcgaggc cttctgcaga
    10621 ttttgcagta agctgggcta tgcgcttggc ataggtttct tgtacgtcga aatgcgctcc
    10681 aatggaggct atatcggtac aggaaacact atagcgtctt gctgcttcgc ttgctgcagg
    10741 aataatgaat ctccgaacaa ggtcgatcat catagcggct tcgattttta tctgcttgga
    10801 gtaacgttcg agatagatat gctgccgagc ctcagtctcc tcaggcaaaa gaacgcagag
    10861 atcggtcaac atttttacat tgtctggctt ggtcagctct tgaatagcat caaccgcgga
    10921 gcggataatc ggtagtcctc tttgctctgc ctcatgtatc cagtcgattg aatatccatt
    10981 gccattgaac acaacgcgtc gatgctcttg ccaggatttt tgcgcaacaa agcgtgcagc
    11041 atcgagcaag ggcatgcctt ttgttaattc tttctcgatc tctctagaga tgtgattcaa
    11101 agcttcagca actgcagtat tcagcatggt aagtggagtt gctgcagact gcgaagaacc
    11161 caccatacga aattcaaatt tgttgccagt aaaagcgaaa gggcttgtgc gatttctatc
    11221 ggagaagtca cgtgagagtt ttggaaggct ccttatgcca agctccaata aggcattggc
    11281 aattttttgt gtattattct ggctttggag cgaatcgaaa aggtcataga gtggttttcc
    11341 aaggtatatc gaaagaatta caggtggtgc ctcattgcca ccaagcctcc ggtcgttacc
    11401 tgcggttgca gtagtagcac gcagaagccc ggcacgtcta tctacagcct cgattaccga
    11461 aagagaaaaa agaacaaatc gcatgaatct gaaccttgca tcttcctcgg atgaagtatc
    11521 cgcgactacg gctgggtcta gaagatcgat acccttgtca gttcctattg accagtttac
    11581 atgttttccc gagccattta agccttcaaa gggcttttca tgtaagagcg cagcaaggcc
    11641 atgatgctcc gcgatcttac aaatggtttc cattacaagt tggttggcat cggcggccat
    11701 atttgcctgt gagtaaatac aggctatttc gaattgatta ggagctacct cattgtgctg
    11761 ggttttagca gatattccca ttgcccagag ctcgctgttg agctcgttca taaaagcggc
    11821 tgccttctct tctatggctc cgtagtaatg atcttcgagc tcctgaccct tcgccgccat
    11881 cgaccctagc actgtgcgac cgcagagctt tagatcaggc cgttttctat agagttcggc
    11941 atttacgagg aaatattcct gctcaggacc agcatagacg ataattcgct cagtttcgct
    12001 gtcgcctagg aggcgcagca aacgaaggcc agcgcaattt agtgcttcca tcgaacgcaa
    12061 cagaggcact ttcttgtcca gagcaagtcc ggtataggac acgaatgccg taggaatgca
    12121 tagaataagg cgatcggcgt ttctttttag aaatgctggg cttgtaacat cccatgcggt
    12181 ataaccgcga gcctcgaagg ttgccctaag gccgcccgag gcaaagctag aggcatcggg
    12241 ttcgcccttt attagatctt tgcccgagaa ctccatcaga atacctccac tgggctgtgg
    12301 tgacacgaag gattcctgct tctcggcagt aagcccagaa agaggatgga accagtgggt
    12361 ataatgagtc gcgccatggt cagaggccca ctccttcatt gctgaggcaa cgatctctgc
    12421 aatgtccagt gtaagctctc tcttgccagc ttgaatatct agaatcgctt caaaagctga
    12481 gcgaggaagg cgctctcgca ttacatcatt ggaaaaacag cggctaccga agtgctgggt
    12541 caaagaaaaa gcatgagaat attgattcat aaaaagttct ccatgaaaaa aagaatattt
    12601 cgtataaaag aagcaagaag tgtgcccaaa ttgggcaatt cgcattatgt ggacgatttt
    12661 aagtttgcga aattcgcttc tatatatcta tatgaggtat aaaatggcat tgtgtgcgaa
    12721 atagtaccat tgactcataa gtattccaaa atgggctctt tccatggaat aataattaca
    12781 aaattgtaag actaagcaaa aacttacaat tttgtcttgg atcggtattg caagattaaa
    12841 aaggacctaa aatttagtct tgaatctacg aggatcgatt ctatggcgct ggatcgcata
    12901 tcgtatcctt cgctcggtga ccatgagccg ccttgctgca gccgcgacat tgccattttc
    12961 cagtttaagg gcttcgatta tgagttcttt ttcgaggcgg aagatctcgg cgtcgagacc
    13021 tgttttgggc tctgtgcctg tcgaggctgc agactggagg cttgggggca aatggtaggc
    13081 gtggattacc ccatcggtcg aaagaatcgc tgcacgtaga atgcagtttt ctagttctcg
    13141 cacattgcca ggccaatggt agatcatgag gagatccaat gccggcgtcg aaatgcgctg
    13201 aatttttttg ctgttttctc gattgaactg cgatacaaag tggtcggaca agaggagtat
    13261 atccgctcct ctctcgcgta aaggagggac atttatggga aatacattga gtctatagta
    13321 aagatcggct ctgaaagagc cttgatccac agcttcctgc agatttcggt tcgtggctgc
    13381 gataacccgt acatcagctt cgatatttgc atttccgccc actcgctcaa agcgatgatc
    13441 ctgcaaaaag cgtaaaagct tgatctgcag agctggtgag agttcaccaa tctcatccaa
    13501 aaagagcgtt ccctcatttg cctgctcaaa tcgacctttc cgctgctcga atgcgccagt
    13561 ataagcgcct ttttcgtgtc cgaaaagctc gctttcgatg acagattcgg gaagagccgc
    13621 acagttcact gctacgaaag gttttgaagc gcgagagctt ttttgatgaa tagcctttgc
    13681 gaccagttcc ttgcccgtgc cactttctcc caataataga acagttgctt cagtatccgc
    13741 gacctgattg atcatagaga acatctcctg cattacatcg gaattgccta ctatgccgca
    13801 ccacgatacc ggagattcgc tcagatttga atcgatagca ttgatgagcc ggccattgtc
    13861 cctatcgatt ttttcggcga gtcttagtcg cattcctagg aattcttcga ttaattctgc
    13921 cgctttctgt atagtctcgc tggcaagcga tttgacctca gaagcgcaat cttcggaaag
    13981 caaaaggctt atcactaata caatattatc cctttgtctt atagggattt cgaggattaa
    14041 acccttactc aattttttct ggatgaattc atcggcttcg agggctagtc cacagggtac
    14101 agaaccaatg cagacttctt cgccaatctt aacattttca tcggaccagg aatcttcatt
    14161 ggggcctata tcgatatatc ggagaacatc gccagaaccc gttcttgaac aggctgcata
    14221 agcccatcct tggaacagag gaagaacgga attgagaaaa cagcccaata cttcctctgg
    14281 ctctccaatc ttgaatagca tcctcaaaag cgccataagc gcatctgagg cagcaacttc
    14341 tatctcagaa aaagaagaat agaatgatgg catcataagg gctcgtttgc agataggaat
    14401 accgagtttg agcctttaat gcaagatggc tggtgaagct aagctttgac tttttcttaa
    14461 taagtatgat ttgccccctt gtgtgaatat aacactctat gatagggtca cttcaccgat
    14521 ggaggagcga ttcccatgag tacggacggg aggccgagag agcctttgaa cgcgccagaa
    14581 aggggaggat cgccgaaggg acagagcctt tctctcaggg ctgttcttgg acgtccgggc
    14641 taatacccga cggctgtcgc ggaaatcaat cgttccgcgg agcgcctgac gtttgccttt
    14701 cttaatgaaa ggagcccagg catggttgct aagcttacaa gcagccttag agcttctttc
    14761 aggaataaga tttctatgaa gggcgcaggc tgacctccac cggaaggtat ggaggtacca
    14821 tgaacaataa tcaatcccaa ttgcaatttt ctccttcgaa aaagattcca gtcacagtac
    14881 ttggtgcaac aggagtggtg gggcagcgct ttctccgtag aattgccaat cacccctggt
    14941 tctatccaca gtttttggct gcaagcgatc gatcggcagg taagaaatat gcagaagcct
    15001 gtcgatggcg tctttcgggc ctttcatacg cagggtatgc agaggcaaaa gtcgtcccat
    15061 gttctccaga gcttgcattt tctcccattg tgttttcagc cctcgatgca ggtcccgcac
    15121 gcgagatcga gccattgttt gcagaggctg gtgcctatgt gttctcgaat gcaagcgcct
    15181 tcagaatgga agaagacatt cctctcctta ttccagagct caaccccgaa caccttgaat
    15241 tgatagaaat gcagaaggct aagcgaagat ggcatggcgc tatcgttact aatccgaact
    15301 gcacaaccgt aatgctcgca gtgcctttgg caattctcga agaacaattc ggccttgagg
    15361 cggttatagt tacaagcatg caggcaatca gtggggcggg ttatccgggt gtatctgcgc
    15421 tcgatatcga agggaatgtt attcctttca tacgaaatga ggaatctaag gtagaatcgg
    15481 aatccaataa gatactggga cgcttagtca aaggggaaaa tgtgatttct attgtacccg
    15541 aatccttcac agtgtcagcg acctgcactc gggtaccggt gatcgatggc cacactctgt
    15601 ctatttccgt aaaattgaaa tcaaagacga gcctcgaaag agtcgtagaa gctttccaaa
    15661 actacaagcc aaagactatg gtctataatc tgccaagtgc tcccaaacat ttcttacggt
    15721 ttctagaaga agacgacagg ccacagcctc gtttagatgc cgaggaagaa gagggcatgt
    15781 gcattagtct ggggagattg cgtccttgtc ctattctaga cttcaaattt atctcgctag
    15841 gtcataatac agaaagaggg gctgctggcg cttcgattct caatgccgag atggcactgt
    15901 ctatggggat gttgaaagag gtcgataata gatgaggacc cttgtgatga aatttggcgg
    15961 tacatcggta ggaagcgccg cccatatcag ggaagtggcc agtattgtgc ggtcacgaga
    16021 gggttccagg tgtgttgtgc tcgtctcggc catggctggg attactaaca cgcttttcga
    16081 gatggcagaa gcagctcggt ctcatgatct agatagggca attcggcttc taggcaaaat
    16141 cgagcaatcc catcttgaag ctagccatga gctagatatc gcaactcccg atattattgg
    16201 taagatcagg gaaatcatcg aaaaccttcg gcgccgaatt catggtatcg atctgttagg
    16261 cgagttcagc ctacgcgtta tggatgagat agccgcagca ggtgaacgct tatcttcttt
    16321 gcttgtctca gaatacataa aatgcccact tcttgatgct cggaaagtta ttcgcacaga
    16381 taatcatttt gggaatgctc atcctaagct accggtcata aaaagaaatg ctgctcttta
    16441 tctagagcct cttttggaat ccagcgatat cattgttaca caaggatata taggttctga
    16501 cgatgctgat gataccacaa cactgggaag aggtggcagc gattattcag cagggctttt
    16561 agctcttgca cttcaggccg atgaaatcga gatttggacg gatgtcgaag gaatcatgac
    16621 tgcggatcct cgcatagtgc cttctgctag gactgtcgat attttgagct atgaggaagc
    16681 cgtggagctt gcttcttatg gagcaaaagt tttgcacccc gccactgtaa ggccagctct
    16741 cgaagcaggc atacctgtca cgatcaggag cacctttgcg ccttacggaa aattttctac
    16801 catcagtcct ggagattcca gtggtcggcc ttgcgtagct gtagcaatgc ggcggcaggt
    16861 tatgatcata tcggttattc aagaatctat gacggatcag gcaggatttc ttgctaagct
    16921 tttcgatatc ttcggaaggc tcggtgtgtc tgtagatctt gtttcgactt ctgaaatatg
    16981 cgtatcggta tctctggata aacgcttgct tttcgaagac ctcttgaaaa acctcgaaga
    17041 attaggccaa gtctctgtgg ccgataatcg tgcagtgatc gctatcgttg gagaccttct
    17101 tcgcaaaacg cccgaggtgc ttagaactac ttttatcgca atggacggca ttcctatcga
    17161 tctaataagc atgggtgcta atgggataaa cttgagcctc attgtgcaag aggctgatgc
    17221 tgagaaggcg attcgcggcc ttcatactgc atttttcgaa ggaggttcta gatgacaata
    17281 aagattggta ttttcggagc aggaaagcta gctcatgcga ttgtcgaaga agtggaggcg
    17341 agtaatgtaa atgaattctt cgtggtgtgg atggtagata aaggcgagat gatccccgat
    17401 agtgatatag acgtcgccat agatgccagc tctgccgatg cagttgagtc gcatatcgac
    17461 tgggccattc ggaaagagat accttttgta attgcaacca caggatggga cattacagat
    17521 ttggcggtac gcattggaga aaaaactggg gtgcttgtaa gccccaattt ttcctttgcg
    17581 gttgccttta tgcagcgaat tgcttcgcag cttgcacgct ttgcagattg gtattcggat
    17641 ggcgaaattg cgattttcga gcaccaccat tcagccaaaa aagactcgcc aagcggaaca
    17701 gctaaggctc tggcacgagc gatcgtttcc agctcgacac gatatacagg atggagcgca
    17761 tctggggtag agaattgtgt caaggataaa attcccatag cttcactgcg ggctgggact
    17821 gagacaggtg ttcacgaaat attattcgat gctccacatg aacaattctc gatgacccat
    17881 cgtgctagag ataggcgggt ctttgcggca ggagcgctaa aagcggctca gtggattata
    17941 ggccgtaagg gcctatttgg tatggaagat gtactgaatg atctttttag ctaggtcaag
    18001 gagataaaga aaagctgaag gaggcaagaa ggttatgaat actatcgatg gtttaggcgt
    18061 cgccattgca acgccgtttg atgatgcaaa taacatcgat tatgaggcat tcgaacgact
    18121 actcgatttt cttgtaggac gagattttga ggcttctgca ggcgaatacg cgcgccgcac
    18181 atttggcggc ggagcaattc ttgagcgctt tcgacttttc tgggagcgcg aatctggcgg
    18241 cgcgcagttt gtcgttgtcc taggttcaac gggcgaagga cctaccgtgg aggccgagga
    18301 gcgtcgcgag cttgtgagga gggctgttga gcgcaagctt ggcattccag tggttgtagg
    18361 cactggctcg aattcgacta aggctgcggt tcgcttgact gaggaggcgg tagatcttgg
    18421 agccgacgcg gtgcttgtag tcgttcctta ttacaataag ccaaccccag ctggtctgat
    18481 tgctcatttt agggcggttg cggctgcagc gagctctaag cctgttattg tctacaatgt
    18541 cccagggcgc accggcatca accttgcgcc ctctgtcatg caacagctct ggaaaattga
    18601 gaatatcaga gcagtcaagg aatcctctgg gaacctagca cagattggag agatttgccg
    18661 agcgcttccc gaaggaaaga ccgtgctctc tggggacgat ggcctcgcac ttccgtcgat
    18721 tgcagtgggg gcagagggtt tagtgagcgt ggctgcgaat gtgatgccga ggcgctttag
    18781 ggcattggtc gatgcagcgc gcgcggggcg ccgccaggac gcaaagacgc ttcatggtga
    18841 gcttttgcct tttatggatg cgctttttgt ggaatccaat ccgattcccg ttaaggcagc
    18901 gctaaagtta tgcgggctag cgggcgatgg gctaaggttg ccgcttgtat cggctacaaa
    18961 gcccacaaag gagcttatgg agagcgtcct ggcggcgctt ggctgcttgc tataatggat
    19021 atggacaatg tgatgaataa agatgctgaa accaatgcga gctttattaa tgtgggcgat
    19081 gatataactt ctggcgcaca aatggataag gttgctcaga ctctccgaga tgttttttct
    19141 gagcaaggtc acttcgataa ggaacagtgg gctctatttc tcgaattatt ggaaaatggc
    19201 agcataagag ttgtggacag gctttcggat ggctcttgga aagtaaatac ttgggttaag
    19261 gaagccatcc tctccggctt tcaagaaggc ggcatggttg aatggtcatg gcaatccgaa
    19321 agcttttttg acaggccggc cttttcgcct cggcatttta ctctttctga tggcgtgcgg
    19381 attgtccccg gaggaactgc ggtgcgtcgc ggagcattta tcgctcgcgg agtggtgata
    19441 atgccgcctt cgtatatcaa tgtaggagcc tttgtagatc aggacacaat ggtggatagt
    19501 catgtgttgg ttggctcctg tgcgcacatt ggcaagaagg tacatctatc cgcaggggcg
    19561 cagatcggtg gtgtgcttga gcctccgcag gcaaatcccg tgattatcga agacgaagtc
    19621 tttataggtg ggatgtgtgg gattttcgag ggtattttgg tcaagaaaag ggcagtgctc
    19681 gctccaggcg taatcataac caagggcacg aggatttttg atctagttag ggggatggat
    19741 ttatatagcg aagtgccgga gaatgcggtt gtcgttcctg gcactcgacc tgctcgcggt
    19801 tcttatgcag agggtctggg catttcgctt gcatcgcctt gtattgtaaa ataccgcgac
    19861 gaaaaaaccg acagtgctgt ggtcctggag tcggctttgc gtggctgaat ggaggaatca
    19921 tgcaatacaa gcttggaata attggtgctg gcaaaatcgg agaggcgatt ctggctggtt
    19981 ctttgtcaaa agggctgctt accccttccg atgtgattct atctgtgcga actgagcagc
    20041 atcggaagaa tctggaggat aaatataatg tgacggcggt gcttcggaac cgcgaagtgg
    20101 caaggcagtc tgagatcatt cttattgcag tcaaacccag gacagtcttc gaagtacttc
    20161 aagaaatagc cgatgtaatt tccgatgaga gtgtcgtcgt ctcgactgtt gcaggagtca
    20221 cgctcgattc gatgcagaaa aagcttgctc gaaaagtggc actaatccga actatgccaa
    20281 atcttggggt gcttgttggt gaaggcatga ccgcactagc accagcacgc tttacagaag
    20341 aatcaaaaat ccagaaagtc aagcagcttt tcgaatcggt tggccgtgct cttgtcttag
    20401 atgagcagta catggatgcc gttacaggtc tttctggttc tgggcctgcc tatgtttacc
    20461 taataattga ggctctaaca gatggtgggg taaagatggg cttgccgcga gaggtctcta
    20521 ttgagctagc agcgcagact gtgctcggcg cggctaagac agtgcttgtc acaggagaac
    20581 atccagctaa gctcaaagac caggttacaa cgcctgcggg ctctaccatt gacggcctga
    20641 tggaactcga agatggtggg cttagagtga cattgatcaa ggccgtggta aaagcaaccg
    20701 agcgagctaa taaactattg cagaagtgaa gactaacctt atgttcaaaa gtcgccttct
    20761 ggctttgcca ttgattttgt ctctgtagag ccatttggtc ttggctctaa gtgctttgtc
    20821 cttatcgctg cttgggcgcg tggcctatct gctattctct tccgagcaat gaagaaatga
    20881 gatttctcaa tagttctgga atcttgagag tttctcctgg ccgaagattt cgaggagcat
    20941 aagacgaaag caatttttgt gcaaaaagct gcgcatctcg gagcattctt tcgctagtca
    21001 cattatcagt ctgctgaatc accatgagta tgcgtctctg tcgcccctgg gaaatcaggc
    21061 ctaaaaggcg ttcatgggct ataggctcga gacatcgcaa tagtgaggcg atttcattat
    21121 cgctcataga agaaagtgtc gtcttgagag ctttcgcttc agcagacata atgatatcgg
    21181 ttgttgtacg gattaggtct ggatcgggct gactcgattc ttcaaaggct tccattcctg
    21241 tcatcagctc tttatccata gctgaacttt cggattcttg acgcgaataa aggtcgagga
    21301 aagcttgctg agcggtacat atttcatcag agataagtct tttgcgcgct gcagctatca
    21361 tctctgtgag tatgaaggaa ccaatgggtc caacaaggct tagaatctgt tctatataat
    21421 atagagagca tcccgcgaag gctgatgctg ccaaaggcat gcctgctatt cggatagtgc
    21481 gctgaaatct cacaggttcg ctgagagcct cttcgatatt gtcggtgaga tctccaggaa
    21541 cgatatattc tgtaagctta gtttcttgaa gctttgattt ctcttggagc catagaaatt
    21601 ccgaatttgc tgtttcaagt ccgcaatatg ctgacatgat taggaatacg atataaggat
    21661 gaatgcctcg agagcatgct tctgtgccag ccaaccacat tagtacttca ggcttgttat
    21721 agtgctgttc cttgaatata aggcccgagc gcaaaaaaga atcgcggatt gcttctggct
    21781 cggtgtcgct tgcaaggagg agataggaaa gcttggttgc ctttctccga gattgttgtg
    21841 atactgcaaa aagctctttg cgattttcgc ttgttttctg gatgacttct agaaactcgc
    21901 gaatcgcttg tatctgctga ttcctttttt tctcggactc gctcgactgt cggggctctc
    21961 cccattggac atacatctta gcgaggcgtt caatcagcat gttctgattt cctttgtgct
    22021 atggtatcta gagccacatc tatgccacag tacggaagaa gcttgcgtag cattatttcc
    22081 tcatcctcgc catttgctat ggaaagaata gcctcggcag caatgcattg tttgagaaag
    22141 tcgatacctt cagccttgct gacgtataga agtaccgcta gaatctcctc caatatttcg
    22201 cctgacaatc cttcgccgac taaaagaagg ccatagcgaa agaagtcgtc tttttcggaa
    22261 gagaattgtc ctgaagtagg ctgtatgccg ccttgcgcaa tcttgttagc cgagcccacc
    22321 aggtaatcga tataagaagg caaaattttc atttcttccg aactgcatct tattttcgca
    22381 gcgagatcgg cgttccagta aatcatttta tctccacata tccatattta tgccagatta
    22441 tagtacaaaa taatgctcca gagaaatggg gcttaaagac atcgctgtca aaacggatta
    22501 gctttctgcc tattgcataa aaacgcatat tatggcatat tatgtgaact atgatgaaat
    22561 ttaacattat ttggtaaaat tttcataatt tttcatatta aatgtaatac caatatattg
    22621 acgcaattat gagaatgtga tatattcaaa ttgaaagcaa taaacgagtg tcggtggcga
    22681 aacgacatta tatagctttt gagcccttca tggtctgcct ccttccatga agggttcttt
    22741 ttttctatac tagttccatg atacttgctg aattcgatac ttgggctcga tcctttcttg
    22801 aaattgatca acttagacag attgacgact cgctcaatgg cattcaggtg gcttgcgaca
    22861 cttcgaagca gatacaaaag atggcggtag cggtcgatgc ctgcgctgag agcattcgaa
    22921 gagcgcgcaa tatgggtgcc gatcttttat ttgtccatca tggtctgttt tgggggaaac
    22981 ccgaaccttt aacaggcagc cttcgagaaa ggcttaagat gctttttgat tccgatatgg
    23041 ctctctatgc atgtcatctt cccctagata agcatccgga agtggggaat aatgctcagc
    23101 tcgcaaatct gcttgggctt aaggagagga aagcttttgg aatatatcat ggcattagca
    23161 tcggatggtc tggtgttctg ccaaagccaa cgaaactgga tcaaatcata gctaagattc
    23221 ttcccgatgg ctcagccgcg aaatccgtaa tcgccgcagg gccaaaagag ataaagactg
    23281 ttgccattgt atccggtgga gccccattcg agtctctcga agccataacg gaaggtatcg
    23341 atctttacat aacaggtgag ccttcgcatt ccatttacca ttacctgata gaaggtggca
    23401 taaacttcat agccgcaggt cactatgcca cagaggtctg gggcgtaaag gcaatagcgg
    23461 aaaaagctca tgcagagttg ggtctcgata caatattcct agatataccc actggattgt
    23521 gagatgaaga aaaaggtgcc tctagcccct cttttgcttt cttggattat aattgttgta
    23581 gatcagatta cgaaggcgct agttataaag tatattcccg aaggaaggat ttttgcacgg
    23641 atttttggag actttgtttg gatcgtgcat actcggaaca cgggagcagc cttttctatc
    23701 ggtgctcttt ctccaccgcc gctcagattc ttatttttta tttttttgcc tattgctgtg
    23761 cttataacat tgcttttcta ttatttcaaa tcggaaaatc tttcgccaat acttagatat
    23821 tcgataggtc tgatagcggg gggagggaca ggaaatctga tagatcgaat tttgcgacct
    23881 gaaggagtcg tagatcttat ttcggtcaat atgtatggtc ttcttggaat ggaacgattt
    23941 gcgaccttca atgttgccga ctctgctatc actgttggcg agatactctt gatacttgga
    24001 tttattatcc tggaatttcg caaacctaag gcgcaatcgc cgcagtctta gaagagatgg
    24061 ggcgctatct tcgtagtatt atgatggatg aggatcaata tgaataagaa acaagccaat
    24121 aaaggtaaag ttgctctctt tgtagtaggg gcaacagtgg cgaatattct tttaatggcg
    24181 atttgttttg tgcttttcat gttgctctat tcggtggctt tttccaagct tcttccgcaa
    24241 gaagcattga tatgggcaat tggcatatca tttttgctct ctcttctcgt atcctcgctt
    24301 atctatcgtc gtttgctgaa attattgagg gagcgttatc acttagatga ttatcttggc
    24361 ttaaaagcaa aatagaaaag ctcggatatc ggatcgaatc ttaggtcgaa ttttggatga
    24421 ccagagccag caaaatgatc gaaaggctaa aagacttagc tatctctatt cctgagatat
    24481 atctgccaaa agaaggcgtc gattatcgga aatgggcggt gatagcatgc gatcaatatt
    24541 cttcagagcg cgaatattgg gagaatgtgg cccaagaagt aggcaatgct ccttcgactc
    24601 tcagactgat ttatcccgaa tgctttctag aagatggcga catggatcag cgtatttcgg
    24661 ccatacatag aacaatgaat gagtatctag aacaaggcat aatcacctca aaaggagaag
    24721 ccttcgtgct agtggaacgt acgactccat tcgaagaaaa gcctaggctt ggccttgttg
    24781 tggcaatcga tcttgaacat tatcagtatg gtaagaattc cagaagtctg atccgaccta
    24841 cagaagacac catagagcag cgccttccgc cccgcatgaa aattaggcgt ggtgctccgc
    24901 tggaactgcc acatatcatg attctggtcg atgatcccaa gaagactctt atagagccgc
    24961 tctatgagca aaggcaatac ttcgaaaagg catatgattt cgatttgatg cagaattctg
    25021 gccatattcg tggttggttc gtaaaagaag agaggcaatt atgccgaatt gcgcaagctc
    25081 tcgaagacct tgctgatcct tgtgcattta gcgaaagata tggctccaat tatagtgcgg
    25141 atgatgtctt gctctttgca gttggcgatg ggaatcactc gcttgctact gcaaaggcta
    25201 tttgggaaga gacaaaaaat gcatataaag ataagccaga tgccggaaga attctctcgg
    25261 agcacccggc gcgctgtgcg cttgttgagc ttgtgaattt acatgacgaa gggcttcctt
    25321 tccatcctat tcatcgtgtg ctctttaata tcgatgctgt ggattttctt cgcaagctga
    25381 gtgctcatgg tgcagagatc cgctacagtt ccaatgcaaa gcaggctttt gcagaggtcg
    25441 atgtaccgca agattcacac ttctttgcat ttgtaactga aaaatggaca ggaacgattc
    25501 gattcgagga gcctaaagcg cggcttgcgg tctccactat tcaggaatgc atagatgaat
    25561 atcgcaaggc gcatccagag gtggttatcg attatataca tggcagtgca agcatagaag
    25621 ctctgggtag gaaaaaagga agcctagggc tttatcttcc gcctgtggac aaagacatgc
    25681 ttttcagtgt tgttatccag gaaggcgtaa tgcctcgcaa aacattttcg atgggcgagg
    25741 ctccagagaa gcgtttttat atcgaatcga ggctaattat accccaaaag taatactctg
    25801 aattgtattg atagactaat gcagaacctt tgcatatata ttctattcta gattgccaaa
    25861 gagtgatatt atccttcgaa actgggaggt ggaaatgctc atactgctag cagatgcttt
    25921 tacgcctgat ttgccagagc gacttgcgga atttggcgaa gtcacttcgg atatgtcgcg
    25981 cattcccgag gctgaggtgc tgatagttcg ttccaagaca aggctcgata gagccatgct
    26041 cgataaggca gctagctgca ggtacattgt caggggaggc gttgggctcg ataccataga
    26101 tgcggaatat gccagatcca aaggcatcat tgtggacaat actcccgaag cctccagcat
    26161 agcggtggcc gagcttgcct ttgctttcat gattgcaatg ccaaatcatc ttgtccgcgc
    26221 cgatatatct atgaaagaag gaaaatggct aaaaaaagaa ctcgagcgga atgagcttta
    26281 tgggaaaacg ctcggtctca taggcattgg gagaattggt cgggagttgg cgcttagggc
    26341 taaggctttt ggaatgtccg taattgcttt cgataaatat gtacttagtt ccgatatagt
    26401 gaagatcgtc agctttgaag agatactggc atgttcggat tatattagct tacatattcc
    26461 gcttatagac gagacaagag gcatgatcga tgcatccgca atctccagga tgaaaaatgg
    26521 ggtctacctt ataaacacct gccgcggtca ggtcgttatc gaagaagaca tagccgatgc
    26581 gctcaaatct gggaaaatag cgggctatgc aaccgatgtt tattcgaagg aaccacccga
    26641 aggctcgcct ctttttgaag cgcctaatgt gcttatgacg ccgcatattg gagcttccac
    26701 cgaagaaaac cttcttaggt tggcggattg tattgttgga aggctcagga aatatgtagg
    26761 tagataagaa atgtggttga gaaaggaaag aagttcatga aacgagcgat taattttaat
    26821 gctgggcccg ctgcgcttcc tatggaagta ttgctgaaag ctcaagagga aatgctggat
    26881 tggcgaggaa ctggcatgtc cgtcatggaa gtgtctcatc gatcgagcga atatgaggcg
    26941 atgcataacg agtcgcagga actttttcgt aagctcgcag gcatgggacc tgagtggaag
    27001 atccttttca tgacaggtgg agcctcgact cagtttttca tggttcccat gaactatctc
    27061 tttagggaac gcaaggcaac ctatattgtg acaggacatt ggggcaaggc tgctgtcaaa
    27121 gaagcaaagc gttttggatc catagatgtc ataagcaccg aaaatgagga tggcagcttt
    27181 acgcgaattc caaagcagga agagctaaga atcgacccta catcaaccta tgtgcacatg
    27241 accagcaaca acacaatcta tggctctcaa tggcaatatt ggccagaggt cggtggagcg
    27301 cctcttgtat gcgatatgtc cagcgatata ttttctcgtc catttccggt cgataaacta
    27361 gcgcttatct atgcaggtgc tcagaagaat ctcggtcctt cgggggtaac attagtcgcc
    27421 attagagacg acttcctcta tcttgcacaa gagcagtcca aactacccac aatgctttct
    27481 tatcgcactt tttcggagaa taattcgcta tataatacac caccatgctt ttcgatatat
    27541 attctgaatc ttacgctaaa gtggctacta tattcgatcg gtgggctcga aaatatgcag
    27601 aagatcaacg aagagaaggc tcgaattctc tatgaggcta tcgactcatc agagggtttc
    27661 tatagagggc cagtggaaaa agaatctcgt tcccagatga acgttgtatt ccgactcaaa
    27721 acgcccgaaa tggaagatgc cttcatcaag caggcaaaat cagctggaat tattgggata
    27781 agggggcatc gttctacagg cggaattaga ttctcaatat ataatgccaa tcttgtcgat
    27841 aatattagaa aagctgtaga atttatggag gagttccggc ttataaaccg ctgaatcctg
    27901 ttagatttcc aatgcaaggg gctggtctgg tctaaggatt cgtaggccag ccttttttat
    27961 tctctataaa tgttttaata gatgttttat atgaaaggat aggttatatt gctttatggc
    28021 taaatcgagg ctgcacagat tttacaataa gttcatctcc tctctcagtt tttcagctga
    28081 gctgcgcaag atgcgccgtg aactcaacgc aaagatcgat cagcccgaaa ttataacttc
    28141 gcctccaccc tttcatcctc aagcagctaa tcgctggttt aagcgccgga gaatttccat
    28201 agcggaatct tacttgatgg tagttagaga cctcgattct cgcaactcgg ccagacgctt
    28261 gaccgccctt aaaaatcttg cagatgtcgc ttttcgttcc tcaaatatag actatccttt
    28321 gaatacagca agggtccagt ctgcccttgt aaaagaagtt gtcaagcacc gctcgaataa
    28381 acgccgccag ctagaattgc tctatgattt ttcgatgtcc acacaagggc aacaccaggt
    28441 aatccgcaaa ctctgcgacg agctcaatat aatagagctg cccgaaaatg gcatgaaaat
    28501 cggagagctt gggtatgcct gggatgacca tgttcacgat gtagcgacct ctggaagaaa
    28561 gaatcctact caactagttc tagatgcctt catcaagggt atatcttcgc tgaccgttgc
    28621 ttacggcctc gtttccgata tcgatttgat ggaggaagtg ctggaagcag gtaacatact
    28681 tggtctcaag gtaaatatag gactcgagtt cagcgttcta gtggaaggag ctcgttacca
    28741 tttcatggcg gagctgcctc atttttcatc aaaggaagaa ttgcgtgctt ttttctcgga
    28801 tcatgcgctg gacatggaaa acttttttca gggactcgaa atcaatagag aaagtagact
    28861 cgatgcggta cgtcgtcttc ttgagagttt caaccaaaat accctgccaa aaataaacaa
    28921 agacttcgag gataaaccag aatattgcct cgccccgctt tccctggaag tgttgcttgc
    28981 aactattccc aatgtaaata ttacgcctct tcatcttgcc gaattcatgt atgtgcgcta
    29041 taggccaatt ctccagaaga gagtgtggta ctacaaagca ctccgcgaaa aggcaaggta
    29101 catggcggag aatacttcta gcctgaagta tggacaatct ggcgaagagc taagagaaga
    29161 gctaagaagg gaagagatcg aagccaaata ttccgagctc aagaaggagt tgaaccaact
    29221 ttctccagac acggttcttt cgacctattt cgacaccccg catataattt cctatcaatc
    29281 agcttttgac gatcttgaat ccataagcca aatgcttaaa aaggcaggat gctccatcaa
    29341 attcgtccag ccgctcgagc atgggctcga tttagctaag caagccttga ttaaacgagg
    29401 tcgatgcata gatgccgtgg aaatctataa catccaggat tgcataggaa gaaatcccga
    29461 agaaatagaa gactttgcgc gttttgtcaa cgaactcaat aaaagcgcag ctgctgaagg
    29521 tcggaattta cttaagccga tctgcggaag cgatgctaca gggagaaatc cgaagattcc
    29581 gggcatggga ttcatattcg aggatcaaat catcggcaat ctgcgcaagc gatacataaa
    29641 acgacatatg cgtctcccat ctctcatctc tgcaatgata cgatccagcg aatctcctgt
    29701 ggacgaggca attctcaaag attctgcggt tcctcgtatc gtctgcatgg gtaagatttc
    29761 tggcagctgg aatcgcgcaa caacaggcga tgaagagaga attggtctgc tgcgtgcatg
    29821 gcggtatttc aatccgacat tcaagaacgt tatacgcgct ataactggat ttaccgttgc
    29881 tacagcctat ataggccctg cttatgctct gctttggctt ggcataacag ggtttcgaaa
    29941 cagtattgcc gacctaattt cctatagagg ccctaaactt agccagtgga aacttaaaag
    30001 catcaatttc gacaatgttg ggcaatcgct tttctggact ggtttttcgg tacctcttat
    30061 gggatatgca aaagctgctt tcgatgcatt gtggccttta gcgcccgaaa cattcttatt
    30121 caatcttgtc aaattctttg ttatttcgat tgtaaacgga ttctaccttg cagcacacaa
    30181 caccttgcgg ggctttgata agagtgtcgt tagagccaac ctattccgaa gtattctagc
    30241 ctggcctcta gccacggtat ttgcgcctat agggaactct ctctcgattc caagcattgt
    30301 tcagaccaag atatggtcag attttgtcgc tggttttata gaaggcggaa ataaatatcg
    30361 caaggtgctt cgacagcgcc aaaagacctt ggaagagctg attcctaata tcatcaatac
    30421 tagcggcaat actcagtaca ttgccatgat ggatatcctt taccttttct ctcaggagcc
    30481 ccgcaccagg acaacgataa aggcggtttt gagtccttat gcattcttta ctcgtaagct
    30541 caaagaaaat tcctcgttgc gcttgaatat gcttttggaa ttgaatgagg taatgagcaa
    30601 ggaaaattta tggacagacc ttgtcgatta catagtgaca aattacgaag aggagatggc
    30661 agatgaactg gtagagcttg tggtcgatgg cttacctgag cttttaaaag atctaagcag
    30721 ccttatagaa aagtatggga aagatccagg tcttcttgca aaactcgcta caaaagcgag
    30781 cggcacaatt agacgatcag ggcagccgaa ataggatctc tatagactag aagagcctat
    30841 aaaaggtcac cgtagcatgc actacatgct acggtgatga gcatcccaaa ttcagaaggg
    30901 tagttctata tcgagtttgc agcgctcctt aacttctttg aaagccttct taaggggcgg
    30961 attgaagggt acacctttgt cttttctgta gagccaggtt tcatgttctt tttcgccggg
    31021 cgtccaaata cgctgagctc ctggggcttt cctcgatgcc ctgagttggc ggcttacttc
    31081 tccgacatga tgtttgaagt cctctaggtc gcagaaggcg cttatattga tggcaatgaa
    31141 gctgtgccct agctcgattg gcttgggttt gccattagag tccacgccca gaagagcctt
    31201 catgaagttg gtttgggaga gagcgctcga aagcagttct actaccatgg cataaccata
    31261 gcctttgtag ccaccgaaat cctcgcctag gcctccaaga ggagtaagag cagccttgtc
    31321 ttggccgagg tctatcagga cctggtgagt atcatgtcga tattttccat cctgtcctat
    31381 tacccatcca tcgggaaggt ccttgccaag cctgtcgtag agctcgatct ttcctcgctg
    31441 ggtaactgag gtcgcgcagt ccaacataaa aggaaagtcc tcgtccgagg gcatgcccca
    31501 tgtcatggga tttgtgccga gcatcggttc gactccccaa gtgggagcta tagaaggtcg
    31561 tgcattggtg gtggtcatgc cgatcatgcc atgctctacg gctatacgcg catagtaaaa
    31621 agcggcgcca taatgtgtcg aatttcggca taccgtcatg cctatgccaa atttctctgc
    31681 tttttctacg gctaattcca tggacctttt tgcgatatag agacccatgc cgttatggcc
    31741 atcgatcact gctgttgtcg gagtctcctt gataacatcg aaggtcgtat ttggattgag
    31801 aattccagcc caaatacgat ctagataaat tggtttatac ctgcccacgc catgagaatc
    31861 gacgccgcgc ttatcagcgg aaatcaagac atcggagcat acagccgcga catcaggagc
    31921 cacaccgacg gcctcgaatc cagccctcat aaaagcatct acttcgtcga atttccacca
    31981 tgttaattca ttgtccatta ctctttctcc ttgtgaatag attaggggct caagctcctg
    32041 tagaacaaga tcctaccaat gatatacatc ataaatctaa aggcgcgata ttgacaatca
    32101 gtattagaaa aacatcgcat aaaaatagaa atgagaaaac ggaagcagaa aatcgaaaaa
    32161 gattgttcgt tccaatttcc attattggtg atacaattct atcgctgcat aagatatatg
    32221 agcaaaggat aaaaaggagg tcagcataat gagtgctcat ccatatacta taacgctaga
    32281 ttttgatgat cttccgccag agcctatttt cgagcctgga atcaggcgag ctccaaagcg
    32341 cgactcggag ttgacacctg gtcaggagag gatcgcgcta aaaaacgcct tgcgctatat
    32401 tcctccccgc tggcatgaaa aactcgcgcc agaattcctg gccgagctca ggcaacgagg
    32461 tcgcatatat gggtatcgct ttcggcccca tggcaagata tgcggccttc ctatcgatag
    32521 ctatttgggt aggtgcattg aaggtcgggc gcttcgcgta atgatcgaca acaatctaga
    32581 cttcgaagtt gcgctctacc catacgagct tgtcacctat ggagagactg ggcaggtatg
    32641 ccaaaactgg atgcagtatc gccttatttg taagtatctc gaagtgctcg acgagaatag
    32701 tacgctagta gtggaatcgg gacatccagt gggcattttt gcgagccatc cttttgcacc
    32761 gcgtgccatc attaccaatg ggctcatggt tggcatgtat gacaatctaa aagatttcaa
    32821 tatcgctgca gcaatgggtg ttgcaaacta tggccaaatg accgcgggcg gttggatgta
    32881 cattggaccc caaggcattg tgcatggcac ttataacaca attctcaacg cgggcaggct
    32941 caaactaggc atcccgcaag ataaggacct tgctggcaga ctctttgtgt cttcaggtct
    33001 cggcggtatg tctggagccc agggcaaggc agcaatgata gctcaagctg tctctatcat
    33061 tgcggaagtg gatcactcgc gaattgaaac aagacttaag cagggctggg tttcttgcga
    33121 aatggagtca tgcgaagaag ctgtccgtct tgcgcatgtt gctcaagaaa agggagagcc
    33181 tattgctgtc gcatatcatg gcaacattgt cgatctcctc gaatatatcg atacccatga
    33241 tattcatgtg gatctactct ccgatcagac tagctgccac gttccctatg atggcggcta
    33301 ttgcccggtc ggcataactt tcgaagagcg tacaaggctt ctagccgagg ataggcatta
    33361 tttcagagct ttggtcgatg cttctttgcg gcggcatttc gaggtgataa tgcaccttgt
    33421 caaaaagggc acatattttt tcgattacgg aaattcattc ctcaaggctg tgttcgatgc
    33481 gggtgtaaag gagatctcca gaaatggcat cgatgaaaaa gatggtttca ttttgccaag
    33541 ctatgtcgaa gatatcatgg gccctgagct ttttgactat ggctatgggc ctttccgttg
    33601 ggtctgctta tctggcaaaa aagaagacct gcataaaaca gaccttgcgg caatggagtg
    33661 catcgatcca gatcgtcgcg gccaagaccg cgacaactat atctggatac gcgatgcgga
    33721 gaaaaacaag cttgtggtgg gaacacaggc acgcattctc ttccaagatg cctttggtcg
    33781 catgaatata gcgctaaagt tcaacgaaat ggtgcgcaat ggagaaattg gcccagtaat
    33841 gataggccgc gaccatcatg acactggtgg tgcagacagt ccattccgcg agacgagcaa
    33901 tatcaaggat ggctcgaatg ttatggcaga tatggcaaca cagtgttttg caggcaatgc
    33961 tgcaaggggt atgagccttg tcacgctgca caatggcgga ggtgtcggca ttggcaaagc
    34021 tataaatggt ggctttggcc tcgtgctaga tggctcggat aaggtcgacc aggtaatccg
    34081 catggcccta ccatgggatg cgatgggtgg cgttgcgcgg cgctcatggg cgcgcaatcc
    34141 tcatgccatg gaggtcgccg atcagttcaa ttgcgagtat ggtgaatatg gcactattac
    34201 catgcctaat cttgtggatg aggaactttt ggaacgcttg cttggagtct attagtaagt
    34261 cggtctatct catgccgtat atcttccatc tcatgataga cagcctttat ggcagcgttt
    34321 tcttctatgc gaccatagac agcgagttgt tcgttctcat tccggaaagt tgtctttctg
    34381 aaaggattcg tccaatcctt tgcccgagta gcatagacgc gctgggctat agtgcatgaa
    34441 agttccttgg cttcttcgag gcttgaaata agctgagaga gagtaggttt tggccatcgc
    34501 tcttctaaat ccgatgcatc taaattcgat gcctctaaat ccgatgcatc tcttctagca
    34561 atagcgacat taaccgcact tgccttttcg ggatcaaggc tcaagtatcc aagaatagcc
    34621 caagcaaaaa ctcgagcatc tttttcatac tctaaggaga gtctttggta ctcgcagtga
    34681 agctcgtccc agctctggat gctacctgtt tcgatgcgct cgatcagatc ttcgagcttg
    34741 gaatgctgga caagaaaacc tcccatgtta tcccattcaa tttccggagg ctcttggagt
    34801 tcagctttat gttggcaatt cttccgttcc ccgagttcct tgctttccag cccgatgcat
    34861 agcgatatcc tctcaaatat ttcatctggt tcagcaaaat catctatctt gtctagttcc
    34921 gttaaaaggg taggcaggag cgtcgtaacc gaaaaccaga gcaatatttc tctataggag
    34981 gctcttgctc tgcaaggctt aagaacgatt tgttgccttg cggaattttc aaagcctttt
    35041 acatatactt caaaaggaag aggatcattt tgtccagcaa ggatgagagc gcctagcttg
    35101 cgaatgccag tattatttgg gttatgaata tcaagattct ccagataagg cctttgtgcg
    35161 gactcattgc tccttttttt ggtgttttca gcttcgattt tttggcttat gtgtaatccg
    35221 agttcgttta caagatccaa aattttgttg ccaaataatc ttcgattatc ctcattattg
    35281 gcgagccatt ctatggctgc ttttccggta gcgtattcaa gaatagcaag gcttgcaaga
    35341 atctctgtca tcgtgtctgg ggcaaatgga gaatatatga actggatgcg cttatcgatt
    35401 ctcttatcgc gctctttgaa tttcttttca tttctcatta gtgcgtatgt gttagaatgc
    35461 caccaccaag ctggagccaa catgagcctg ccattggttt cgtcgttgtc tacaaggcag
    35521 aaggggaaac gaatatcgag ttcatgcttg tagtcacttt ttgaaagaag gcagaatgaa
    35581 gcgaaacgcg atgaatgttt cactgaggtg cagaggccag gccaaaaacc tcgccctgct
    35641 tctatttccc catccggagc ccgcgaattg tgattcgatc caattgtggc accggcggcg
    35701 aggttggatt gaccttttat gagacttgcg atcaagaagc ttgtgttatg gtgctgctcg
    35761 tgggctggaa aaatcaggtt atttagaatc tcgcagcagg atacggtgct attttcacca
    35821 aggacagagt gaattagtct ggctccatat ttcaaggctg aattatcgca catgacaaat
    35881 cgcactgcct tgcagccgta aaagacacgg cagccatagc ccaaaatgcc atttacaagc
    35941 tctacacctt caccaatctg cgtaggtgct tcggttttgg aattaattgt gagatttttc
    36001 agcttgttcg agccttttat ataagcgctt tctccaatgc atacgtcttt gattatgcga
    36061 ttgtgcttga gtacgctgtc gcgacctatg attccgtatc ggccgagtct gcgatcgaat
    36121 tttgtgttgg tcatctcagc aaagcgctcc ataagggcat ctctgtcgcg aaatttagcc
    36181 caaagccagg catccgcagg aagcatgcca ttgaatggat aaatccagcg gccgccgttc
    36241 tcattcataa catcgagcgt tatgcgaacc gactcctctt cgctctccat gacaatacca
    36301 tttccgaact ttgcatgatt ggagacctgc aattccgcat tgttgatgag aatacagtga
    36361 tcgtcgatga tgtagtgaga aagataagca caatccgaaa tggcacagtt atctccgatg
    36421 tcgcaggcta tgaccagcga attgtaaatt ccggttgtgg tagagaaatc atggtgctcc
    36481 aaagtagcgg gttcaaggcg ggatatgcgt acaagtccga agaatatgtt gtttctcaaa
    36541 agctcaggat ggaacggatc tcgcacaaga aacaaagacc aatccctgca ggtgttattg
    36601 tttctcacaa gaacttctaa ttcgcttgct gaaagcggcc tgaaagataa aggctccttg
    36661 cctaggcttc taagttgttc atcgcgtaga tagtattcat tttcgccagg gcgaagatat
    36721 cgagatggaa caaatccttc tcccactctt gtgttttcga gaatcttaat atcgtttgcc
    36781 atgctacaag tatgaaatac tctttgtttc catgcaactg gatgaatggg gtattaagcg
    36841 acagattaga tcttctccta tcccccttga ccatttcatg ccttttcgtc atggtattgc
    36901 catggatttt gtcgattccg ccagagtgga cactctcgta accggatccg agaaaaaagt
    36961 ttttgagctt ttcgaatcta tggtaaagtc cacaggccaa gaacgaaaga aatccgcact
    37021 cgctctagcc aatcttttag gaaaccccgg agaattcagt ttctatattg actgcacgga
    37081 agatcagcgc ttaattcgag tctttcatct tttgcgtgta ttccgagaaa acatgacact
    37141 cttaataaat aagacctggg ccgatggaag ccagaatacc actcaagaag aactgcttgg
    37201 agatcttgcc cgattcatcg aagaatatag ggattttcgt attgtctcgg cttttcgtac
    37261 cttcgtagga attgcgcgtc aaatcccatc tctgctgttt ggatctcttg gaaaggcgaa
    37321 tgattttcta gaatatgcat tccgtatcga tccaaaattt ggtcttttct tctggtacat
    37381 tggcgaaatc gagcttcagt tgaggaatat cgaaaacata ccagagcaca gggaactttt
    37441 cgatcttgag gtcttaattg gagcctttgt gctttcctgt ttctagtttt attctttaca
    37501 atatagagtc cggaatacat tccataataa ggtcttcaga taagaaaggc ttgtctcaaa
    37561 gtcttcgagg cctacgggct tttctccttg tttacaagca tatcttattg gaagaagaca
    37621 ataccccgaa actagattct taagtctaaa ttcactcagc agcaccaatc cgcaggatgg
    37681 atcgaaaagg aggattcctg ccgattctgt atcggcttca gtatctgatt caacatcggt
    37741 ttcggcctcc aattttgaat cggatttaat atcgataatg atgacaaagt gagaaaactg
    37801 gccacggaca tgaaggatca gaggagcatt tatagatttt acgagctcat gcaaagcttc
    37861 aggcgaaaac ttaaacacca ttgaatcgat tccatgatca gcaataatgg ccttgatatc
    37921 ggccagcgaa attggcggta ttttcccata gtttcgggat agagttttat cattttcgaa
    37981 tgctggcata catggatttt tgtgtttttc tctctctaaa tcttccaagc ctgccaagaa
    38041 aacactagtc cgcataatgt tgatcaaggc cgctgcaacc gcatagccgc agctcgattc
    38101 gctcgtttga agcggaagca tggcagtgac taaactcatt gccatagcag gaatctgtga
    38161 aatttgccag aaaagcatat cgtgcctcct ccaagagaag acgtgaacag aaagctcggc
    38221 tgcttcaact tttgaggcaa atgttatttt gttgggatta ttgccaatta atctcgttta
    38281 ggctcgaaat ggctgaattc catgctaaag gaagctcgac cctggctcac ggatctgagg
    38341 cttgtagtaa agccaaacat agtggcgagt ggagcctcgg catgtattat ctcggctact
    38401 atctttgtct ctgtgccatg gataagtccg ccgcgctgag aaacaaggct cataacctct
    38461 ccgacgaatt cctttggagt cagaatatcg actttcataa tgggttcgag caaaatggga
    38521 tttgctgctc tacatgcggc atcataggcc atattggcgc aggcctcgaa ggcaaaggca
    38581 gtggagctaa gctcgttata ttcgatatcg atgagtgaaa cacttatgtc tacggctgga
    38641 tatccatact gaatgcccga agaaaaggca gacattatgc ctcgttctat agcttcgaaa
    38701 atttcctgag gcacatctgg ggcgcgaaca agttttttat actgattgcc ggttcctctt
    38761 gggagcggtt ccacttctat cgttaggcct gcagtattct cttttccagc aataatcttg
    38821 tggaattttt cgctatggcg atatttagtc gaaatcgatt ctctgtaggt gacctgtgga
    38881 ttgccgatct tggcttggac cttgaattct cggataacgc gcgtgacaag cacatcgaga
    38941 tgtaattctc ccattcctct aataatgatc tgaccggtct cttcatcctc atggactgcg
    39001 aaagttggat cctcgcgagc taaaatatcg agcgcttctt ttagtttgtc tcggtcggaa
    39061 agcgttctag gttcgattgc taccgagata acaggttctg gaaagtgcat cttttcgaga
    39121 agtacgggat aaccttcggt gccgagagtg tcgccggtct gtgccaattt gagccctaca
    39181 aatactgcaa tatcgcctgc gctaaccgat tcgataggct cgcttttgtt agcgtgcatg
    39241 cgtaaaagcc tagttatacg ctcgcgcttt ttttttgagt tgttatatac aactgaggag
    39301 ctgtgcagcg tcccagaata catgcgcaca tagcagaggg cgccggcttc gcggtcatat
    39361 tgaaccttga atacaagtcc aagaggatat ccgcttatct gacaaggaat ttctatttcc
    39421 tgctcttttt tagcatgata agctttgatc gaaccaactt catccggagc aggcagaaaa
    39481 tcgactatcg catcgagaag aaattgaacg cccatgtttc tgcgcgaagc tccggcaaat
    39541 actggaatga agtgctgagc gatgcaagct tttcgtattt ctgctattaa ggtctctttc
    39601 ggtatggcga caccttcgag atagagcttt gttatgagat cggactggga tgagactgca
    39661 tcaagaagat tttctcgcca tcgatgagcc atttcttggc gctcgggagc aatatccgat
    39721 cttgtcatat cggatccatc ttcgctccag tatatctctt gcatttcgag gagatcgatg
    39781 actccttcga aagaactctc ggccccaatt gggatattca agggcacagg atgagcaccg
    39841 agttttgtcc tcatgtcctt caaaacggct tcgaaattgg cgccgactct gtccatttta
    39901 ttgatatatg cgatgcgcgg aacgcggtat cgatctgctt gatgccagac ggtttcggac
    39961 tgtggctcta cacctcccac ggcgcagaac actgctatag ctccatcgag tacacggaga
    40021 gagcgctcga cttcagcggt aaagtccaca tggcctggcg tatcgatgat atttatctga
    40081 tgatcgcgcc aaaaggtggt cgtggctgcg ctctggatcg taatgccacg ctcttgctcc
    40141 tggtccatcc agtccattgt agcttcgccg ttgtcaactt cgcctatttt atatgttttt
    40201 ccagaataat agagcatacg ttctgttgtt gtagtcttgc cagcatcgat atgcgccatg
    40261 ataccaatat tgcgtatttt ctccaaatcc atggattgcc ccggagcaaa tataaggatt
    40321 gtagtttctt ggaacttccg taaggccgga agacaagaag aaaagcccaa aaaaccaggc
    40381 cataaaaata tataggaaaa gtgaataaaa tacaacatta aggcaagcgc tgttatgccc
    40441 tgctatgccg atggcgccgt tacacttgct gcttataaaa atcctataat atccatatca
    40501 tagttggctc cattattatg cccgtgtatc tcatctcctt gcaccaaagc accattaaag
    40561 cttataagga tactgtgaag aaagttgaat tgcttgcgcc agcaggatca cgagaggcgc
    40621 tcgacgcagc tattggcgaa ggagcagatg ctgtctatct tggcttaaga tccttcaatg
    40681 ctcgtatgcg tacgacgaat tttgccttta accaattcga ggctgcagta tatgcatgcc
    40741 attatcgctc gcggcggatc tatgtgactg tcaataccgt attcgaagag cgcgaatcgg
    40801 atcgcatcta tcagttgcta gaatatctcg ctaccgtagg accggacggc ataatcgttc
    40861 aagatgcggg tgtcgcaaag attgcacatg actattttcc ttctttgccg cttcatgcat
    40921 cgactcagat gaatatcgct tcggccgcag ggtgcaatca gctttcgcga gcgggcttta
    40981 agagagcggt actcgctcgc gagctttcgc tcgaagagat tcggcagata aggcagtata
    41041 cgagtcttga gctcgaggtc tttgtccatg gtgcgctttg tgttagcgct tcgggacttt
    41101 gtctattttc gagctatctg ggaggcaaaa gtgcgaatcg aggtcaatgt acacaagcat
    41161 gcagaaggct ctacgaaagc gaaaaaggta agggttatta tttttcgccc gacgatcttg
    41221 aacttataga ataccttccg gatctaatca cggcgggagc ggatgccttc aaaatcgaag
    41281 gtagaatgaa atctgcggaa tatgttggca cagtggtatc agcctaccgc tttatgctag
    41341 acaattacga gaaagataga gaaagagcca tactaaaggc caaggctata cttcagtcgg
    41401 attttgctcg ccgaaaaacg aactttttca tatctggcaa gtccgaagac tacattcatc
    41461 ccgagcagtc tggaggcaca ggcattgctc ttggcaagat tcgcgatgcc agaaccatcg
    41521 atggcaagcg ctgggcgtta ttgaacagct acgaaggctt ggctgaacga gattccgtac
    41581 gcatccatcg aaaagacgac tcaggccgca taaccgctaa aattcaagcg atacgatacg
    41641 aagtatatgg aatgctcctt ctattggatg gagactggcg gcaaaacgat gagctatacc
    41701 tcatccaaac agccagtatg acgcggaact ataagccagt gctgcccaag aatctcgata
    41761 agtttcataa atttccgagc aaccatgcgg ctccgcgccc cgcgctttta cgcctcgatg
    41821 gtagaaaact cgaggccttg ttcgaacctg gaaactatgt ccttgcgggt aaagttgctc
    41881 acctgcacgc cgcgctgacc tttcgcccca agaaggccat gatccttttc aataaactga
    41941 atgcagaaac catgcgccgc gaagaggaaa gccttccttt taaaagagat aggcttattc
    42001 tctggctcga tccatatctt gccgagtccg attccacatg gcttaccgct gaactcgatt
    42061 attggctcga aaagggtgtc tcacttgtaa ttgtgaacaa tcaagcgcat ttttcgatgc
    42121 taagagggaa aaaggtcatt tcgattgctg gcccatggct ctacacattt aatcaatggg
    42181 cgcttgctta ttatatcgag cagggagttc aggcttttat tccgccatac gagatttctc
    42241 ggcaagatct ttataggctc acagaatacc ttccagctca attttttgct cccatagttt
    42301 ttgcatatcc cgatttattt agaataagag cagacttgtc gaaaaaatat ggccaggcta
    42361 attttattga tcgcgaaaac aacactttca gacttatagg aaagcgcgac tattctgtgg
    42421 ttgttcccga aagacctttt tctattaccg atttggtgcc gaatcttaag aagcaaggat
    42481 ttaggcgctt cattgtcgat ctatcgaatg cagagccatc caaaggccta tatcgggata
    42541 tcgcacgagc ggtggatcaa ggcagctcgc tactcaatac tacgcgcttc aactggaaag
    42601 aaggtttttg gagcgaagaa acaatacgca gggatgcctc caaggatgac tccaaggatg
    42661 cttccataaa taagaaaccc aaaagtccac cctatgctca acggcgtcaa tccagaacca
    42721 gataaagaag gaggatgtgt cgacggatta tctggatatt tgcatatatg aacggtttaa
    42781 attgaaatgc tttagcgggt gcacgacaat tttgtggcaa agatagccta ttccttgacg
    42841 acacatctca cagcgtataa agttacgaga taaattgcct ttttatatgt tgatttatta
    42901 aaagcgaatg gcattttttg gagttgcaaa gcatgagtga atcggctggc aaggcctttg
    42961 atcttctatt ctatctcgca aaaacagggg aagcggtctc tttagcccgc ctttccaaag
    43021 agacaggtat gaataaggcc acagcgctaa gatatttaaa tgtcttggaa tcgaagggtg
    43081 tggtggagcg ctgtaaaatg ggctggactc tcgggctttc gcttttcgag ctaggtagca
    43141 aagtaccagt gcgccaattg gtagcggata aagtgcgccc tatcatagag aggctcgctc
    43201 gagaaactgg cgaatccgta aaccttgcct atcttgccga tgaaacagcc atcttcctcg
    43261 atcgtgcaga ggcaaaccgg agccttcgta tgcgatccat gcctggagat aggcttcctc
    43321 tctattgtac gggagtgggt aaggcgattc tttctcagct tagcgaagag aggatatatg
    43381 ctatcctggg tccaggacca ctggaaaaat ttaccgaatc cacctatacc gatcctgagg
    43441 acataatcaa cgaggctaga cgcgctagag aattgggcta tggtatagac agcgaggaat
    43501 tcgaggttgg gcttacctgt tatgcaatgc ctctacgcat tcccagttcg gactttgttg
    43561 gtgctataag catctctggc cctaccgctc gcatgaagaa tccagaaatc cgcgatcgct
    43621 tcatatatct tctaaaacaa gcaataaaag acgctactga agtgctatct cctcattgtt
    43681 atttggacaa catatcgaag aaagccaaga gctaaagctc tattgtaatt ttgacagagc
    43741 gtgccacgat tttagcagag ctcatggaca ctctctgtca tggtagagtt ctagaatact
    43801 ggtgtctcta tttcaccccg ctttgaatct tcgatgaatg gtgtttcgga atttggggct
    43861 tctgcctgcg ttgctttgct cataggtttg aactccacat gctcggcaac aattttgact
    43921 cgagagtgtg attttccttc ggtatctgtc caacgatcct gttttagcct tccaacaacc
    43981 ctgacaccgc gtcccttttt tagattctgt gcacaggcag aacctagtct tgcccacgct
    44041 tcgacatcga aatatgacac ttcattttca tacttttggt ctgcgatctt gtagtttcga
    44101 tttgtggcaa ccgtaaaatc acagacctga tttccagatg gcgtcgatcg aaaattagga
    44161 tcgcggacca aattaccttc taccagaata ctgttaaggt gattcatacg acctccttgt
    44221 gtactagccg tgcgtcgaga ttacaggagc atcattgctt gcctgcatga ctaattcgca
    44281 aggaatccat atccatgatt caatttttat gccctctttg ctaatatata cgcatggaaa
    44341 gctttgatcg atttcaatct ccttttacat ggcgctatgg aagcgataaa atgcgagaaa
    44401 tctggagcga gcataataag cgtaaactct ggcgtcgtat atggatagct cttgcaaagg
    44461 cagaatcaag ttatggcctt gtaacgcctt ctcaaattgc cgagctggaa agccatgccg
    44521 atgatatcga tttagaaaaa gccgggaaaa tcgaagattc gatacaccac gacctaatgg
    44581 ccgagctcca agtttttgct tcgcaatgcc caagcgcagg tggagttctc catctgggcg
    44641 caacctcaat ggacatagag gataatgcag aggttatgag gattcgcgag agcctttctc
    44701 ttctacaaag aaagcttaca aaactcctcc tcaaactggc agagaagata gaactctatg
    44761 catccttgcc tgtcatggcc tttactcact tgcagcccgc agagccaacg acaatgggat
    44821 atcgctatgc ttgttactct caggaccttg tagatgtcta taagcgcctt tcttatacga
    44881 ttgctgatat cagaggaaag ggcttcaagg gtgcagtagg cacttctgca tcctatgttg
    44941 agcttttagg gctagacaag ctcgatgatt tcgaacgagc tttatccgag aatctaggct
    45001 tacagttttt cgatgtcgcc actcaaacct atacgcgagt acaggattat cggataattg
    45061 ccctcctggc cgagatgggt gcgatatttc ataaaatggc ctttgatttt cggatcttgc
    45121 agatgccgat gctaggcgaa atccgtgaac cttttggaga gcatcagatt ggctcttccg
    45181 ctatgccctt taagcgcaac ccaatcgaat gcgaaaaggt agattcattg gctcgacttc
    45241 ttttatctta ttcgaatgtt gcatgggaca atgcagcttt gtctattctc gagcgcacgc
    45301 ttgacgattc agccaatagg cgcatcgttc taccagaggc attccttgca gcagatgagc
    45361 tgattacgac tatgcagcat attatcgatg gccagattat cgataaagaa gccatccagc
    45421 tcaccttgga tacttatgcg ccattttccg cgacggaacg gattttaatg gctcttggca
    45481 aacggggtgc ggatcggcag atttcacatg gacgccttcg ggatatggca atgctggcgt
    45541 ggaaagcagt aaaagaagga aaaaccaatc cacttaagtc tctcatcaag tccgatgaat
    45601 tttttgcttc atttctttct tcagatacta tcgaaaaact atttgatgtc gatatttata
    45661 taggtgcggc cgagcaacgt gcgctagcag tggcagagcg catccgaagg ttcgcgatgg
    45721 atgaatgctg aattgagcga tatactttta agatgctgat atttttctca aggagttgac
    45781 atgttagatt tcatcagcaa gggtggaccg atcctatggg tcattatggg actatctgtg
    45841 gtggcactcg cgattgtaat agaacgtcta ttgtatctta gacgtatttc gatcgatgaa
    45901 gaaaagctgt ttctgcgcat aaaaacaagc ttgatggaag gacatttttc cgaggctctt
    45961 gctatatgcg accaaaatat ttcgcctttt tcgacattgt ttaaggttgg gatagaaaac
    46021 agaaagcagc ctgaatactt gcagcgagag cttttaaaag atgccgcatc tctggaatcg
    46081 gtcaatttgg agagagggct tactacgctt ggtactatct ccaacatttc aaccttgctt
    46141 gggcttttag gaacggtcac cggtactatg cgtgcctttg gcgtcttagg gaaattcggc
    46201 gctgtaagtg atcccgctgc gctggcaagc ggagtggcag aggctctaat aacaacggtg
    46261 ggcgggcttg tagtcgccat tcctgtcatt atgatttaca actattttgt atcgagggtt
    46321 aatttgatca tgacccgcct cgagacacag gtgaataact tggtgagctt tatctctagc
    46381 tatggtggga aggaacctga cagtgctgag gagaagccct cgaaggaggc atgatggctc
    46441 gcaatcacaa actttcgcca aagatcaatt ttgatcttac gcctctaata gatatcatac
    46501 ttcagctggt gatttttttc atgattacaa caactttcag gactgctcca gggataaact
    46561 tgcagcttcc ttcttccaag actgctcaga ctatttctac acccgaacta agggtagtag
    46621 ttatgtcctc agatgaaatc tatgtcgata agacgaaaac caacctgaca agccttgagt
    46681 cggtgttagg caagcgcgtg agtggctcgg atgctaagca gattcgtgca attcttgaag
    46741 gtcgagcagg agcggaatat cagcttatta tttctgttct cgatgctttg cgaatgaatg
    46801 gtatcgagaa tgtcgggctc ataacgagaa aagaaaaggt ggttagatga gcgaggccct
    46861 cacctatgcc gagcgagaac agcgaaaacg caccattctc tcatatatta ttgcaattgt
    46921 tatgtacgct attctgtttg gagcaggcct tctattcaat attcttaagc cagagagcat
    46981 cgatttcagc aataaatccc ttatagtgaa tattcagggt ccagttacga acgatattgg
    47041 cagaggttct ccgatcgaaa aagaggaagg gcttgttgca gaaaaaccag caccgcctcc
    47101 tgcttcagta ccggagccta aggcccaaac tagcatcaag ccaaagacgg cagagcctgt
    47161 cgcgacaagc cccgctgcca agcctgaacc tacaccgact cccataactc cggagacaac
    47221 cgcaatccaa gagccagaaa aaccctggat tccaggcgag cgtgggcctg gttcgcggat
    47281 cagttcgagc gaatcttcgg tgctgtcgcc aggcgaaggc caggttccat ggggcactgg
    47341 agaagcagtt cgaatcacca aggccgaaaa aggaaacact gtagaaacta ctttaggcgg
    47401 atcctcagaa agtgtgggtc aaagccttta tgttccaata tatttgagca tgcctcttcc
    47461 ggaggtcgta ccagccgcac tgttcaacgc tatacctcct gaaatcgttc ctccaaacac
    47521 acttattgct tccgccgaat cgcgaaagcg agccttccta aattattatt catggaatgg
    47581 caaggaatat cgtctaacaa atccagtacc cttagaggtt cgcgataaac tctgggagat
    47641 gctggaggat gccggctata acgttgaaaa agccgattac aaagctggtc gttcgctaaa
    47701 tccaatagtc ataggctttt ccattaccaa agatcgtcag ctgagagggc ttgaaattct
    47761 gcaatcctcc ggagaccccg aaatcgatgc tgctgtgctc tatggcttca agcgagcatc
    47821 gttttggaat aaaagcgggg ataccattca ggggcgcttt gtctatagat tctgatgtta
    47881 tactatcctg ccttataatg tgttataatg tgccccaata actcatagca taatcatcga
    47941 gcaggagcaa ttcgatgata ttcaattttt cttcgctcat cgctgcatta tcgttctgca
    48001 tctatgtcat tttcatagta tttggtcttt ctggcagaaa agagcgtgtg agtgtttcat
    48061 tccttatata tatggtcatg atggcgctct ggagcttcgg cgcctttatg gcctatggca
    48121 atataggctt aataagccta accaactcaa tcaagatcaa gttggttggg ttgcttggag
    48181 cacctttcgc catttttgcc acacttctat atttttccaa gagcgagaaa aagctatatc
    48241 gaaatttgct ctatattgga tatatcattt atgcatatct gctctatctg aatttttcta
    48301 gctccattgt tgcagatgtc tggttcgaag gccttgatta taattatagg cttgggtcag
    48361 atgcgcttat agcctatttt ttctcttatg cttatttgat tcttactatc ttttttttat
    48421 taagggaaca aaaggctaat cagaacaagt ttttaaagcg cgctctgcag ctaatgctga
    48481 taggcgtcgt ggtgcttatg atcggagttt tagcttacct atacgagcct attggccgct
    48541 atcctgtgga catgattgca gcgatgataa actcgtcgat tatattttat agcatatata
    48601 aatatagact tgtacaatat tcggctgccg tgttcgattt gattctcggt gcgcttgtag
    48661 cagccttttc aagcattata tacatgatgt tctttgtttt cattttcagg ctcaatcgct
    48721 ttgtgccaaa ctccactcta gttcccttgg ctctttgcct tggtttatgc tctgccttct
    48781 tatttggacc attgcatacg gcaatacaga ctctttttga acgtatatcg ggtagaaaaa
    48841 gttttctcta ttatagagag ttaagggatt tttctactaa gctcacatcc atcgtgagcc
    48901 tcgaggagct ttcgaggttg attgtcgata atgtgatatc ggcattgaag ctcgagtggg
    48961 catttgttct tataaacgaa tatagatcgc agaatttccg agttatcgct tctcatggct
    49021 tggattttat acaggaacaa ggaagccaga acccaactaa tcaggtcatt gtgcccaagt
    49081 catctccttt catccaagcc tatcaaaaga aagctgctca agaaaagcaa gcctattcac
    49141 agtggaacat cggcataatg ttgataaaag gccaaaagca tcagacagtc ttagcgagtc
    49201 tcgtgcttcc gctaaggttc aaagaaaggc tcaatggctt catcattctt ggtccgcgcc
    49261 tcgagaaaga ctactacaac aagtatgagc tcgatatatt gcaatttctt gtggatcaag
    49321 catcggttgc aatggagaat gctataactt acgagcgtct acgccagcat caaaagcgcc
    49381 ttcaagatgc caacaaacag ctaatcaatc agagaaaatt cgaaaccttt ttcgatggaa
    49441 ttgcaacgcc tatttcgatt caggacatca attacaatat tgtaagcgct aactatgctg
    49501 ctcagcgtta tttcgagaag actcaggaag agctaatagg atctaagtgc tacaaggcat
    49561 atttcaatag agatcgccca tgtgccgaat gccttgctca ggactgcctc catgcaatgc
    49621 tgcctttcaa taccgaaaag caagacagcc gaactcaact tacgttctcg ctcaattttt
    49681 accccattcc cacaagggaa gcttctatgg gggctttcat cgagcttttc caggatataa
    49741 ctaagcaaaa aagtcagcat gaagagctcc tttcagaaga aaaactcgct gctattggtt
    49801 cgctcgtttc cacaattgca catgaaataa acaatcctat tggcggaatt ctaggtacga
    49861 cggatttgat tctggaggag acaccggaag gtagcccaat ccgcggatat acagaggata
    49921 tcgcccgtta ctcccagaac gccttggatt ttatcaagag ccttatggtc tattcaaaga
    49981 agacgcgaac tactttagag atgataaata ttataaatgt gctcgagaac tcgttgaaaa
    50041 tggcaatgcg cagtatcgat ttcggatcgg ttgtcgtgcg aaagtcctac gatagtgtca
    50101 aggaggttct tgcaaatccg actgagcttc agcaagtatt tcttaatctc atcatcaatt
    50161 ctgttcaagc tatgcgtagc gatggagtct tgacactttc ctgtcggcag gaaagagatg
    50221 atgtgctggt ttcggtccaa gatactggca taggaatgag gagtgagaat ttcgatagaa
    50281 ttgtaaataa tttcctaaca gttgaagaaa ccagctcggc tactggaatg ggcctgagtt
    50341 ttgctaacca tatcgtaaca aaatatggag gacgtatcct cctcgacagc cagcttggga
    50401 agggaacgac ctttactgtg attctcccat ccgcaatcca agacaaggat aggatatggt
    50461 ttatccatgc cagcgagccg cgccaattcg atgacagcta ttatctacag ctcaaggtat
    50521 tggtgggaga aaagggttat caggaagaaa caattcatcg caaatgcgac gattcggcct
    50581 ttcatattat tgcctacaag gggtttcagc ccattggtac agtctctgtt cacttgagcg
    50641 aagcgaatgg gcatattcct atcgaggaaa acttccaaat cagtcctttt ctcaggggcg
    50701 ttccctacgc cgagatcgac aggctcgcag tgacgaagga cgaacgccta ggtcttactc
    50761 cattcgaaat aatggtgctg tcctatctat atgcccgcgg ttggggcgca aaacagattt
    50821 ttatcgatgt ctttgccgat gaagctagtc agatccaaat gttcaagaaa gttggcttcg
    50881 agattattgg aagttacagc aatccgctgc catgtactgt catgatgatg aaccatgctt
    50941 cgatatatga gcagaagact ccaaataatc agctctttat taagagaact atttcgcgcc
    51001 ttattcccaa gatcgatttt tcagcagagg aattgtcttt ggtaatgaaa gctatcgatg
    51061 aaatcaatgc catatttcca agacaagata ctgtagacac taaagagata tcatcataca
    51121 atctatcatg atatgaagcc agaagattta gcagagggac taaacatcat ttttgctcac
    51181 tctcctgttt tctctgacca gtctttaatt atcgatgttg ttgctaatcc aaaagccggc
    51241 ggctttagta gaatccacaa ttcaaataag cgaatgagag agttaaagga gatggtaaag
    51301 cggtcgtccg atctacctct ccgtaaggct ccttttagct tgaaaatgca tctcacagag
    51361 aggtgtggac acgctgcgac aatagtccag cgtatactgg agcattctcc gtctaacggg
    51421 aaagatacct accacctcat cattacagca ggcggagacg gcacaagtct cgagactgct
    51481 gagcaactca ctctgcttcc cgaatcggaa aaggatcgtt ttggaatagt aaggttgccc
    51541 ttcggaacag gcaacgatgg ctcggaagga agggatcttt ttgttgctct ggagaggttt
    51601 cttaatccag ctattctcga gcggaggcct gcgctcaaaa taacgccaag ttatgaaggt
    51661 ggggccctgc cacgctattc tttcaatata gctagtgtag gtctagatgc ctatgtagcg
    51721 gagatgacaa atcgcctcaa gcgccatttt cctggagatt cctacaagtt ttgggtcgat
    51781 gtagggacta tgttctatga tcgtgtctat aaagtggtaa acatgggtct cagggcttgg
    51841 aacagaggta ggcttgtact ggattccgag cttcctcgtc ttttggtagc tatgggagct
    51901 tcgggcaacc gttattatgg ttcgcataag agcatacttc ccaatgaaca caactgcatc
    51961 gcaatatctc agactagcct gtttagaaag cttattctaa aaggatccat agagcatggg
    52021 aagcacgacc acattcgtga actcacacat ttcactgcgg acaagctcca gatagattac
    52081 cacgaaagca ttcctctaca gtgcgatgga gagacaacta tccttgcaaa atgtgacttc
    52141 cctcttgaaa tagagaaact tcagaacgcc tataatgtgc ttgtaccagt gtagagcgca
    52201 aaacgactta ttctggaata gactgcgcca ctaggactaa gagtcgaccg tagaagctcg
    52261 aacgaatcga tagataggct cagttttggg ggtctaggaa gcgacgaaag ctcaggagca
    52321 ataggtagct tgagtcgtgc gagtgtaaga tgggccttcc atgggcgagc atcggtctct
    52381 gctcctagct gaattgcaat ctcacgtatc ctgtttatca atattgtaag cggttcgaat
    52441 ggttctatgt gcgtcgcgag atataatact cgaggcctat tcatggatgg gagcatggac
    52501 agatcttctg tagttatcct gagcggaggg cagagattgg ccaaatccga aagcaaaatc
    52561 ttgagctttt cagcatcttc tgcttccaat tccccaaaga aatgaagagt caaatgaaga
    52621 ttttgaggag gaacccaaga gggacgagct ggcctaaaag cctcctcgag cgaagcgcag
    52681 taagctccta tctgattctg ggtagcgact ggaaaaggca aggcagcaaa gagacgcatg
    52741 atttattatg gcagataatc cttccttatg cgagacatag actcaaagat attgcgcttc
    52801 ttgtggctcg agccctcggt caaatgttcg aggagttctc ttgctttttt tcgttctcct
    52861 tgcaaattga atgagcatgt acacgtgtaa gccgacaagc ccagttcatc ggcacaagct
    52921 agaatttgcc tttcttcgca caaagcaaga ggccggataa ctgttatggg atattttcta
    52981 tagggcatga caggaggcat cgttgcaaat tcgccctttt tcagcatgtt cataagcaga
    53041 gtctcgagaa tatcgtccag atggtgacca agagcgagct tgttgaatcc atgcgccatg
    53101 gcatatttca ttagttcgga tcggcgctgg gttgtacacc agtagcagtt cattgacctt
    53161 cctggtttga gtctgcccac cacaggtatt tcgagtcgtg tgtacggaat accccattcg
    53221 gtcatacgca aagcaagcca ggcatcatcc tgagtctgag aaaagcttgc atcctctagg
    53281 tcggtgacta tatgacaggc ggcaatttcg aaaggagttt cccaccattg gcgtttcttg
    53341 gccaggtccc aggcaaggca gcaggaatct tttccgccag agactgcaat tagtattcta
    53401 tctccaggct gaatcatact gtagcgacgc agggcaatct cgctaagctt agaaagagtg
    53461 gtgcgagcgt ttttcatgtt attttaattt ggatcaatgt cgttctatgg ttatgccagc
    53521 catagatgtg ggctcggatt tgatatcgga gtcttgtccc tcaagagcgg aatttggctt
    53581 ttcatagcct gtagatggat agatgacgga attgatgaag gaaacgagta catctagcgc
    53641 ccgttcatta aacccaccct cgggaataat tatgtcggcg taagccttgg tcggttcgat
    53701 aaaatcgtag tgtccaggtc ttaccattgt catatattgc tccaccacgg agtcgacagt
    53761 tcggccgcgc tccttgatat ctcgcaatag gcgtcggatg aagcggatat cgtccggtgt
    53821 atcgacatat atctttaggt ctataaggtc tcgcacttct ttgttagtaa aaatcattat
    53881 tccctcgaaa attacaagct tggagggctt aatatggata gtctgacctg ttcttttgtg
    53941 atgaacgaaa tcgtatacag gcatgtcgat agattggcca tttttgagcg ccgtaagatg
    54001 agatattaga agctcgttat cgaaggcatc gggatggtcg aagttgaacg ccgtgatatt
    54061 gaggttgctt atgaattctg ctgacttgta gtaattatcc tgtggcagga agacgaagtc
    54121 gctgacaatt tcagatattt ttcgaacgat cgtggtttta cccgagccgg aaccaccaga
    54181 aataccgatt atcttgactg ctggcatacc cttttttatc acggctttgc attatggtaa
    54241 agtggaaggc tattcgctct gagccatccg aacaagcatt tcttgaattt catccacaat
    54301 gaagtcggga gtggaggcac ccgccgtaat gcctatgcga ttatagcgca tcatctcggg
    54361 cgtgagttcg cttgctgtct ctatatgcca ggaaggaaga ccaaagccta tggctgtttt
    54421 ataaagcctt ttagtatttt cagagttttt gcctcctact acaagcaccg catctacctg
    54481 tgtgctgagt tctgccaatg cctgctggcg ttctcgggta gctgggcata tggtcttttc
    54541 tgcgatcatc tttttgacat aagactgaaa gatatcgatg atcgcatcgt agtcttcctg
    54601 acggatagtt gtctgcgcaa ttagggcaca aggtttgtct ctgagtgccc tagccatagg
    54661 aagggctgtt tccgggctat ctactactag agcattcaag gtatgtccta gaatacctga
    54721 aatctcgcca tgacgagggt ctcctatgag cactatttgc caacctaatc tttcgtaatg
    54781 ccgggctaga ccttggctct tgatgactcg ggggcaagtg gcatcgacta ctcgtgcacc
    54841 aagcacatgc aagcgagaaa gtgcggccag cgatgctcca tgagctctga ttataactat
    54901 agcatctttg aggttcggaa gttcatccag ttccgactcg attgcgctag gttccagaac
    54961 gcgaacacca agagattcaa ggtcggcaac tgcttgagga ttatggataa gtgggccata
    55021 gctatagaca gggcggccag tttgagcggc tttctcggca gctcgatgcg ccagctcatc
    55081 tgcgcgtcgt acgcccatac acataccaag gattcttgct cgaacaatct gcatagttcg
    55141 atattatcaa atttggaaag ccatttccac aatgcgcatt gcggattttt cgaaaagtgt
    55201 tatactacta tttctggaaa tttcgctatc tagaaaagga gaattcatgg caactcgcaa
    55261 gactattgtc gtagcgcttg gtggcaatgc gattatcgaa gaaggcagcg aaggaactat
    55321 agaacaacag ttcgaaaaca cccgaaagag catgaaggca atagttggaa tgatagccga
    55381 aggccacaaa gttgttctta cacatggaaa tggcccacaa gctggcgttc atctcatccg
    55441 aaacgaggca gcttccgcac aagtccctcc atccccactc aatgtaattg ttgccgatac
    55501 gcaaggttct atgggctata tgatcgcaca gagtcttgct aatgcacttc gcaccgaagg
    55561 catagataag gatgtcgtca ctgtaataac gcaggtagtt gtcgacccga atgatccatc
    55621 aatgctgaat ccaactaaat ttgtcggtcc tttttacaag gccgaggaag tagagaaatt
    55681 acgcgcccgc ggctggataa tcaaggaaga tccgatgcgc ggctatcggc gtgtagtccc
    55741 aagcccaaaa ccgctcgatg ttatagaaaa ggacacgatc aaggatctca tagacgatgg
    55801 caagatagta attgcggttg gaggaggtgg aattcccgtc aagcgagatg cagatgggat
    55861 tctctccgga gtggatgctg tgatcgataa agatagagcc tcgtcgctcc ttgcgagtct
    55921 tattgatgcg gacgagctca taatacttac aggtgtagat aaggttgcaa taaacttccg
    55981 aaagcccgat cagcgagttt tcgatcatct aagtttaaaa gaatgcgagc aatacatggc
    56041 cgaaggacat tttccaaaag gttccatggg gcctaagatc gaggcagctt gtgactttat
    56101 tagaagaggt ggtgctaaag tgattataac ttccatggga aatgccactg ccgcggtaaa
    56161 tggcaaagcc ggcacggtga tcacagctta gtgcaacttg accattcgag ctgctccatg
    56221 gtaattttgc tggaattctt tttcgataag gaagatatat gaatggtatc cagaatggcc
    56281 ttttcctgct tcagcagaca acggccagca atccgacggg gtcgttaatc tcgacgatca
    56341 ttaccttcgg tctcgtgttt gtgatctttt actttttgat cattcggcca cagaacaaaa
    56401 agcagaagca gatgcagcag atgatagctg gagtcaaaaa gggtgataag gtcgtaacca
    56461 tcggcggcat ccatggtgtg gtagcttcgg tcaaagattc cacagtggtg cttaaggttg
    56521 atgatggaac gcggatcgaa ttttcaaaat cggctatagc ttccataagc cctgtcaagg
    56581 ctgaagaaaa agccgaagac aagccggacg aaaaggccga agaaaagcca gaggacagcg
    56641 ctgaatcgac ccgaaaaacc gataaagaaa agaccgaaga caagtaaaga tttcattgtg
    56701 caatacccag ctttcgctcg ggtaggcata acacatttct ctccatttaa tacatccatg
    56761 agaggattgg agtaacgggt atgagcaaat tgcgccgcct catgatagta attgcagtac
    56821 tagccgtggc atttatgttt ctttttccga gtattcagtg gtatttcctg acaccgaaag
    56881 aggatcaagc cattgcggta ggttccagag agcaaataag agaatattcc cgtaggatga
    56941 cctatctcat aatcagcgat ctaaaagaaa gagcagctgc gggcgatac
//
LOCUS       DAOSOB010000005        51852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_694_length_51852_cov_63.852096, whole genome shotgun sequence.
ACCESSION   DAOSOB010000005 DAOSOB010000000
VERSION     DAOSOB010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 51852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51852
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_694_length_51852_cov_63.852096"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            443..1060
                     /locus_tag="PLS88_01030"
     CDS             443..1060
                     /locus_tag="PLS88_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05975.1"
                     /translation="MEIQLQELLERIHSEGIENAKQQAEEIIQKAHSEAAEIVARARR
                     DAEEMQSEALKRIDAMEAASRESLLQASRDTMIALRQSVQRFLDSAIRADVKKAFDEK
                     MAAQVIPEVLKILSSGQSGDFEVLLPPEQFEKIDSSLSARLAKELSRGVSFKPYDAID
                     AGFRVALSGSSVQYDFSAESIAQMLSVRVNKLLSQYLKEAAGSLD"
     gene            1062..1652
                     /locus_tag="PLS88_01035"
     CDS             1062..1652
                     /locus_tag="PLS88_01035"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOU05976.1"
                     /translation="MKHYWYFASTLTSFPFGSPPPVSVQAFDELCSRMIDKSDLEIFP
                     CIEEARNGRFSESMKKSAFLMSYFEFERGLRNALVVFRTRASKQDASQWMRPGDINPD
                     SLKAAQAAQAASDPLQAEFAIERERWNAVERLSALSAFELDYILAYKMKLLIASRYAS
                     FDRNRGMQGFNILYQDIVNAAAAVDAASAFDTGVAT"
     gene            1649..3412
                     /locus_tag="PLS88_01040"
     CDS             1649..3412
                     /locus_tag="PLS88_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOU05977.1"
                     /translation="MTEGFVTAVNGNMVSIQTEGIVTMNEVAYILVGERRLKSEVIRI
                     RGTSVQAQVYEITKGIRVGNRVEFSNELLSVELGPGLLGQIYDGLQNPLPEIANKVGY
                     FLEPGLYLRALPRDIKWHFTPLLRKGDVLKRGMGIGWVPERYFRHIIMMPFDLYGEWK
                     VIEIAPEGDYTIDEAIAEVADSKGNKRHIRMCFSWPVKRAVDCYSERLKPSEPLVTKT
                     RIIDTFFPVAKGGTYCIPGPFGAGKTVLQQVTSRHAEVDIVLIAACGERAGEVVETLK
                     EFPQIIDPKTGRSLMERTVIICNTSSMPVAAREASVYTAVTIAEYYRQMGLDVLLLAD
                     STSRWAQAMREMSGRLEEIPGEEAFPAYLESVIAAFYERAGIVRLYNGKEGSLTIGGT
                     VSPAGGNFEEPVTQATLKVVGAFHGLSRERSDARKYPSIHPLDSWSKYDGIMPKSTVR
                     YAHSFLERGAEISAMMKVVGEEGTSLEDYVIYLKSEFLDAVYLQQNSFDPVDAAVSPA
                     RQRHVFKLILKILAADIRFSDKEEARNFFYELRQTMLDLNGTPQDSDMFNALYNRVDA
                     ALVDREPEFDNRGLKLMSTLE"
     gene            3416..4714
                     /locus_tag="PLS88_01045"
     CDS             3416..4714
                     /locus_tag="PLS88_01045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOU05978.1"
                     /translation="MNKIYSRIESIVGNVIAVEAQDIAYGELAQVNTRYGASLAETIR
                     LDGKMVYLQVFSGSRGISTGDEVRFLGHPMQVSFSDDLMGRIFDGAGEPRDGGPDLTE
                     NLITIAGPAVNPYQRIIPREMIRTGIPMIDVFNTLVKSQKLPIFSVSGEPYNPLLARI
                     AMQAEVDLIILGGMGLKYDDYLFFKDTLEQGGSLSKTIMFVHTAADPIVECLMVPDIS
                     LAIAERFALKGKNVLVLLSDMTNFADALKEIAITQEQVPSNRGYPGDLYSQLAARYEK
                     AVDFSDAGSITILAVTTMPGDDVTHPIPDNTGYITEGQFYLRDGHIEPFGSLSRLKQL
                     VNAKTREDHRSIMDAMIKLFAAYRDTLEKKSMGFQMSAWDRKLLKYGELFEQRIMDLS
                     VNIPLEKALDLCWETLAECFEPRETGLRSDLIQKFWIGKK"
     gene            4748..5362
                     /locus_tag="PLS88_01050"
     CDS             4748..5362
                     /locus_tag="PLS88_01050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOU05979.1"
                     /translation="MGKIKLTKNELKKKRDELKMYERYLPTLQLKKQQLQVEVRSVQT
                     RMEELQAQKTTLEQEASSWMAVFTEQTAARTKEGEPILKVSSISSDTGNIAGVAIPVF
                     LGVEFDHKSYDLYATPLWVDNALILLERLVRTKIELDIVSKQKELLSQELRITTQRVN
                     LFEKVKIPETVADIRKIRIYLGDQETAQVVRGKIAKRKVEGAER"
     gene            5371..7248
                     /locus_tag="PLS88_01055"
     CDS             5371..7248
                     /locus_tag="PLS88_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05980.1"
                     /translation="MKKFYLIVLQRDRENLPERLRNLGVAHVEELRGIGEKYQGLERE
                     KAEAESVYFLLQNYVDKKSKPSKEDTSISLKYEEYKDVRDIIREISAAKQRLDLLEDR
                     SIDLKREINRVESWGEVSMAMLEQIIDGVGLTLRFFEAPLKELATLPKETEYIRISAP
                     KGRCRIGLILHEGEETPELPPSFLEFLPPEIPLSQMRQEIASIDSQKQQILREFRENS
                     ANLSLIKKYLHKLNSSIILERLRSGMPQKESLAYMKGYVPARDCEKFKKAAAKYGWAI
                     AFDDPIEEDQPPTLVENPPAVRIIQPVFEFLGTVPNYWEYDISSWFLIFFSIFFAMIF
                     GDASYGAIIVLATIASIIAGKRKGKPIGDAQKLFLLLGSTTVIWGALTATWFGIAFDS
                     LPRLLQNISIPSINGQSPDSENNIKVICFSIGLIQLSIAHLKNLKRDFPDLKFLSQAG
                     SLLLLLGMFNAALNLVIDATRFPITNWAIVCIAVGFFLVFIFGNWNGKLGSSLLESLK
                     NIIPTFLGTVSVFADIVSYIRLWAVGLAGLALAQTVNGMAGGLLSGPSKFIIDLILKF
                     IIAAVILVAAHALNFLLTVLSVIVHGIRLNMLEFSGHLGMEWSGYKYDPLKEDEDLSA
                     S"
     gene            7275..7697
                     /locus_tag="PLS88_01060"
     CDS             7275..7697
                     /locus_tag="PLS88_01060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455682.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOU05981.1"
                     /translation="MNIALIGAACVLGFGAIGSGIGAGIAGMAAIGSWKRSYLNNKAP
                     SFLLVAFAGAPLTQTIYSFILMTRIINSTKEPLLLLAAGIMSGLSEGISAIAQGQAAA
                     AGCDAFGETGKGFANYIIVVGLVETVALFTLAFTFSVI"
     gene            7732..8817
                     /gene="ispG"
                     /locus_tag="PLS88_01065"
                     /gene_synonym="gcpE"
     CDS             7732..8817
                     /gene="ispG"
                     /locus_tag="PLS88_01065"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOU05982.1"
                     /translation="MKETHIIKVGDLFLGGDWPVSIQTMWKDALPRFSSSGDAQLLDI
                     LEKLSSLKKLGCDIIRFAVPEISTAESLGMLAGLSPLPIVADIHFDWRIALRCMDFPI
                     AKIRINPGNIGAEWKVREVVAKAKDKGIPIRIGINAGSLPRDLAMEQDVVSAAIKAAE
                     REIELLESMSFNDIVVSLKMNQPDEVVRANEEFSLRYPYPLHLGVTEAGPLVAGIVRN
                     TAALVPLLKKGIGATIRVSLSDTMEQEVIAGKEILACVGKGKHGIRIVSCPRCGRASF
                     DTHAFVSRWMNRLYSLNADISIAVMGCIVNGPGEARQAALGITGAGNSVLIFKHGEII
                     RRVEKEQADIAFEEELESLLKDEINSN"
     gene            complement(8871..9218)
                     /locus_tag="PLS88_01070"
     CDS             complement(8871..9218)
                     /locus_tag="PLS88_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU05983.1"
                     /translation="MNQTTKTCYEEKLEPYAQKFKAIGHPIRLKILCLIADQEVPCVG
                     DIWRCLDQPQPVVSQHLAILKHYGIVTSEVKKTRRIYSISDPFIKNLIDAMICQIKAE
                     SNSSSEKMAEKSE"
     gene            complement(9328..9729)
                     /locus_tag="PLS88_01075"
     CDS             complement(9328..9729)
                     /locus_tag="PLS88_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05984.1"
                     /translation="MLVLVLIEEKSIANVVLRQLNHKDIASIAYRDPLRVLDHLTELN
                     PEAVIIRGKDFPLHQQLLAALVRFYAPLKGCKMIVLGRGIPDFPPCICIAEEQFLREP
                     SIITNEILKTHHIDSKTGSRLVAKAQRTMKL"
     gene            9810..13205
                     /gene="mfd"
                     /locus_tag="PLS88_01080"
     CDS             9810..13205
                     /gene="mfd"
                     /locus_tag="PLS88_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOU05985.1"
                     /translation="MKKDNLQALFEKISKNPALGKIQKLVEQDSFPIHIQNSENSLLA
                     LSLASLVRRTKRKCLVVLPSDHETEEFSQDLALTGVDLLDFPLWFGAPYRALPPRSRM
                     FRERASALARLAEGDFEIATVSARTLAIPVPPPEYTRNHILPLRIGADFEPIAISSKL
                     AELGYLRVPSVSLPGEYAVRGEVLDIYMPGYEYAVRIHFDYDRIERIVRFDPETQTGR
                     EQITQIHVRPLKELLWGKAEIAQLQNNASDFVRTDPRIRQIIENLDSGKEMLGEELWF
                     PLAFDRINNLMDYLDNKAMLVLVASERIDAISETLRKEYASMYRIALREMLVPPPHKI
                     HINLDGLSAVTSSVLYCHALKGIDGSVERISLEAEPQHSYFGNIRLFKEELSGFKKED
                     YKTWIIASTEPQAERISSLIQDDSIIISTGPISAGFTIPSLRLRVLAEHELFGRRKHV
                     PRSIAKTKSSVIESFIELTPGDYVVHTNYGIGKFIGIERLIVLGLERDYICIEYAADE
                     KVFVPIEQANLVQRYIGNEGEAPHLDSVGSKVWENRKKRVSKSVEELAERLIRIYARR
                     KSAHGFAFPSDNEWQIQFEATFPYEETEDQLKCIEEVKHDMESPKPMDRLVCGDVGFG
                     KTEIAMRACFKAITAGKQVAFLAPTTILAEQHFENFLERIGDFPIKVALLSRLVDRKT
                     QNSIIKGLADGSIDMVIGTHRILQNDVHFKDLGLLVVDEEQRFGVKDKERLKEMKVGI
                     DCLTLTATPIPRTLHMSLLKIRDMSVLQTPPLERQPIQTFVEEFSPELVARAIRNEIA
                     RGGQIFYLHNRIETLPEVEYFIRQLVPEALVESAHGKLDGKDLESIMHRFIHGAFHVL
                     VSTTIIENGIDIPNVNTIIIDRSDSYGISQLYQLKGRVGRSDRQAYAYLLYPDKRALS
                     EIAMKRLQIISDFTELGSGFKIAMKDLEVRGAGNLLGREQSGNIYAVGFDLYLKLLDE
                     AVSRLSGIAEEEEEPYLELEYSGFIPDSYISVPMIKMEIYKRIASIRTQEEIDSLHEE
                     LEERFGPLPDEALSLLSLAEMRLLCRRLSIGRLQERAGIVTVEFSKVSKISVEKILRL
                     IRESAGTIRLSPEKPNAIQIQTRAIGLKEKSAYLRERLSLLAG"
     gene            complement(13169..13975)
                     /locus_tag="PLS88_01085"
     CDS             complement(13169..13975)
                     /locus_tag="PLS88_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOU05986.1"
                     /translation="MSETEKSSSTLYLIPAPLWPYSPENWMPCQLMNMIAPNVLTLMR
                     KVPYFVVESQKTAERLLSRLLSPEQFSKVHFFILSEHSEAQDLKAPIDALKSGYDCAI
                     LSEAGLPCIADPGAALVYLAHEERLRVVPIGGDSSLLLALAASGLNGQRFCFLGYMPI
                     QSEELKKELAREGKAAMHDEAARIFIETPYRNAKTLKACIEALPENIYLCVAYDLGTN
                     QPLIRSMSVASWRKEAFILPEQPAVFCFGLPMNLGKKLLQPARRERRSLR"
     gene            complement(13999..14835)
                     /locus_tag="PLS88_01090"
     CDS             complement(13999..14835)
                     /locus_tag="PLS88_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOU05987.1"
                     /translation="MNLSEIYYPHSVQELIVIMERQKNIQIVAGATSFGYIQEPRYLS
                     FEPSIACIRNVPELKTIHKTERMISFGAACSLSELESVYPFEKNPAREVLRATANSAV
                     RNIATIGGHLMYQKRFLALWPLLACLDTELEFKGPSGTSIKNIWYLTNEEGLPTLESR
                     RILTRIRIPLQSIDYLFIRRTGGGIFPDGDGAYLVSACSIERNSISNFKLVIAGQRAY
                     RDYDAEQKIVSSPYPLTERVFQSALRMYSESLLRTNFWNTELFVPLISQALDNLQRSS
                     IL"
     gene            complement(14832..15311)
                     /locus_tag="PLS88_01095"
     CDS             complement(14832..15311)
                     /locus_tag="PLS88_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU05988.1"
                     /translation="MNIEFTLNGNLQRITITSVERAFDVLINQCGIKSFSEISPDRDC
                     SNCLILYDGKPVCSYILPAFLLREQHVETIEYFAKSKEYDLLSAELEKEGLLSCTYCN
                     NSLMLVAMELVHRSLTPSLYEISDILASLHCQCIDPRAFANTLLKLIQQLAIARKLP"
     gene            complement(15301..17052)
                     /locus_tag="PLS88_01100"
     CDS             complement(15301..17052)
                     /locus_tag="PLS88_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU05989.1"
                     /translation="MTINECIPFEQLSAPGMLYAFNIRALSNIGEIQNIKVPPLPEGY
                     TLITADQLPFRNELKIKGISLPLLASKKVSYIGEAVGLIVGPDPLIAEELALKTIVDC
                     EGQESRLDWQVFSSSDISARIDYSKENAMVSSESPESSGAEDSFELASYLRLEPREFS
                     FHSGLGALAEWQDDRLKLACPTLWPELLHSCIAELIGSSIENIEILQSKMYDNSGIYF
                     WYPSLLGARSAAAARALKRPVKLLISIQREKRFLPCVHGISLHMRTKWSQYKHNLMNI
                     ECRFAIPIGAYSVLDELILEKTVHLVADAIPNIPLTIIGFAVRTNTIPMGAIDCISSS
                     AIFSMLETHIARAARKMDLEFLELLCITLGKRDSYSAITKMPAKNESSTEKIVKPLLE
                     HTDFHRKYAAYEQIHKRTRGAREARIRAISLSIADQSHIDSSSISMITDLAQGAAILE
                     VDYDSLRSIFRNIRLDISIYAGKILCPFKTKGAIRSASIEALLSCIRRSFYPDIDIND
                     IYEWIISESRINIELVKDETVNSTLPIGDLAYTLVLSCFLEVMQQLNSSERLILPLRP
                     TPTAISSTELLEKDDEH"
     gene            complement(17039..17593)
                     /locus_tag="PLS88_01105"
     CDS             complement(17039..17593)
                     /locus_tag="PLS88_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOU05990.1"
                     /translation="MRFYAIQVTTRKEDEWLERIQHQVTNLKFHNIMKKMYIRKRGKT
                     KLENSPVFPGYIFFEYNGESLPLETIYRLRHSQFFIRFLPNNEAPQPLNNRDSEIIRH
                     FINFGSLIPPSLVRFDENQKIKVIEGPLQGIEGFIIKVNKRKRRAKVKLDIAESVIIL
                     DLAFEVMEADTAETAPQGIYYDNK"
     gene            17706..19640
                     /gene="ligA"
                     /locus_tag="PLS88_01110"
     CDS             17706..19640
                     /gene="ligA"
                     /locus_tag="PLS88_01110"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOU05991.1"
                     /translation="MPNDLKANEDIQVRQKRIRELESLIRHHQELYYNGEPEISDEEF
                     DALWDELASIDPENPLIASIGADRSDRWPKYRHRMTMGSLSKATDPESFLAWAAKVKY
                     PLYLVQYKLDGASIELQYDEGAFARGVTRGDGVIGDDITPNAIRMNGVPKQLQEPFTG
                     SVRGEVLMSKRVHAIKYSDKANCRNAANGLMKRKDGQGVEDLDIVCYDAVGRLPAGEA
                     FSTEREKIVWLKRMGFNVVPTVECKNPEEIIVYRAKIMELRPTLEYDIDGLVIKGDRI
                     DPEDMNKLRPELQIAFKFSPEEAVTTLIAVEWSESGTNVTPIGIVEPVRLAGTTVQRA
                     NLANPGMIRSMNLKIGSKVVITKRGEIIPKIESLVENPKDALPISIPSHCSQCGAELI
                     DEDTRLYCPNPECPGKAYHRLEKWLSVVDIKDIGSTLLRRLFEAKRVMRIPDLYTLTM
                     DELISFERMGEKSAQKILRNIKGRNEVSLSQFIAGFDIEGIGLLMADKLVEAGYDTLD
                     KILAASPADFEKIEGFAEITAKALYEGLHLVEKDMRELVDRGYVRIKPPLRRIQRGTN
                     PIAGKSFCFTGELRSMKRSDAEKLVREMGGIAKSSVTKDLDYLVTNDPSSGSEKNRKA
                     QELSINIIDEKEFLEMIGLK"
     gene            19651..19962
                     /locus_tag="PLS88_01115"
     CDS             19651..19962
                     /locus_tag="PLS88_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOU05992.1"
                     /translation="MKAPSIRTQEHLDFLKPALHEEKEGVVLEVYVVPRAPCDSVEEL
                     RNGRLLLRVKAAPEKGRANEAVLELIANYLDMAPSNLRILRGNSSRRKSILLQNFRYN
                     R"
     gene            19976..21748
                     /gene="argS"
                     /locus_tag="PLS88_01120"
     CDS             19976..21748
                     /gene="argS"
                     /locus_tag="PLS88_01120"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOU05993.1"
                     /translation="MEYYVDQWKSIIANCLNELAFQKELKDAYLSSQDIIAEKPPRPE
                     FGDIGFPMFSYAKQFRMPPARIAAEVANVCNIKTDTEKPIWPGTVKTAGPYINIYLDK
                     KYVANYVLENAEKEFWESSPLFNGRRIMIEFSCPNTNKPLHLGHLRNNVLGESLSRIM
                     KEAGAEVKKVNLINDRGIHICKSMLAYLAYGEGRTPEDEGLKSDHFVGKYYVLFNRLK
                     DEDPSAEEKAQELLQKWEAGDADVIALWEKMNNWAVEGIMATYRRQKVSFDEFDFEHE
                     TYKLGKEEVLEGLARGIFYRAEDGSIQVDLEDIGLGKKVLLRKDGTSIYITQDIGTAI
                     MRYNRWPFHELVYVVASEQQYHFKVLFEVLRRLGYNWAENLYHLSYGLVNLPSGRMKT
                     REGTVVDADDLIDELTRLAANEIADKRREEAVGDIKDVAEQVALGALHYFLLQVSPAK
                     DMLYNPEQSISFTGDTGPYIQYMGARASSILRKYKNGEGNANQGKINVSLIVSDSDWS
                     LMRLLIEFPHTIEQSAKNKDPSILAAYTHQLASEFSVWYRDNPVLNNDNPDISASRLA
                     FVIAIRETLRRASNLLCIPFLDAM"
     gene            21869..22180
                     /gene="rplU"
                     /locus_tag="PLS88_01125"
     CDS             21869..22180
                     /gene="rplU"
                     /locus_tag="PLS88_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOU05994.1"
                     /translation="MYAIVEINGKQYHAEQGKDLVVDRFDAMPGQSIVFEKVLLLSGD
                     ETKVGSPYVNDAAVKAIVKEEIKGDKIIVFKYKPKKGYHKTRGHRQPYTVLTVNEIVG
                     A"
     gene            22211..22456
                     /gene="rpmA"
                     /locus_tag="PLS88_01130"
     CDS             22211..22456
                     /gene="rpmA"
                     /locus_tag="PLS88_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOU05995.1"
                     /translation="MPHKGVNGRDSNPQYLGVKAYSSENVNAGSIIVRQRGSRINPGK
                     NVGKGKDDTLFALIDGTVRFREYKGKKFVDVEPAIQA"
     gene            22543..23649
                     /gene="obgE"
                     /locus_tag="PLS88_01135"
     CDS             22543..23649
                     /gene="obgE"
                     /locus_tag="PLS88_01135"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOU05996.1"
                     /translation="MIKFADEAIISVASGKGGDGCVAFRRERFIPFGGPAGGDGGRGG
                     DVIFIVKQNLRTLAYLRYRKVFKAENGQPGMGKNMHGRDGKDVIIEVPPGTILRDAET
                     NEIIRDFGRRAGGKRAGTLEYVEPWVFLKGGKGGWGNTHFKSSVNQTPRYAQPGQPGQ
                     ERKLKVELSLIADIGFVGFPNAGKSSLLEYFTNARPKIAPYPFTTKIPNLGVLTVNER
                     DIILADIPGIIEGAHEGAGLGLRFLKHIARTACLAFLVDLSDEHWRTAFPILLHELGL
                     FSPTLVQKPRILLGTKMDLPESQTNFMEFKASFPEESVYGISVFTGQGLDEVKQQFFE
                     KVLAYEVAEAYEAQKENAPDRLFDEIDFSDEGFI"
     gene            23649..24227
                     /gene="nadD"
                     /locus_tag="PLS88_01140"
     CDS             23649..24227
                     /gene="nadD"
                     /locus_tag="PLS88_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOU05997.1"
                     /translation="MRIAIIGGSFNPPHIGHMILIEEILATECYQRVLLIPAKAAPHK
                     VPQMDPGPEMRLALLEASIEGWHNVAIEDCELRRAGLSYTSDTLEELWNRRLFDERPG
                     LVIGDDLATDFLATWKRAEKILELADIIVAHRLYVDKIPLPYPHNYIENLIIPISSTL
                     VRERIHAGGAWRSLVMPSVRTIIENYGLYRNT"
     gene            24205..24819
                     /gene="yqeK"
                     /locus_tag="PLS88_01145"
     CDS             24205..24819
                     /gene="yqeK"
                     /locus_tag="PLS88_01145"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOU05998.1"
                     /translation="MDYIEIPEIAKVIEKEIACTLTPKRYAHSRSTAETAVKLAERFG
                     EDRWAAHLAGLSHDMCRGLSFEEQDNLVNRHKACIAFLSKRASLAAILSDKEFKEKMI
                     HGPAAACLLCHRFQVRQEPILEAVALHSIADDAMSPLAKIVYIADKLEPLRNRAADAD
                     EKMQTLDLDSLFAYTLTSVVKWFSESGRPLCPYTAEIYSRMPKL"
     gene            24816..26006
                     /locus_tag="PLS88_01150"
     CDS             24816..26006
                     /locus_tag="PLS88_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOU05999.1"
                     /translation="MSDSKSKILKRSSLPLLVILIVVAIGLFFVVKLTEKGTVESIIK
                     KDQPMAILFIFESDKKPVSNQLLIWYPSRRKAAIMDIPSTMGIILKSANKMSSIDTVY
                     DSRNPSKFVKEISDYLKYPIDGWFVYDEVSLCQTIDLLEGIQMFIPETVIESDSLKGV
                     SLPGGAVVLDGDKTNQYLLYALATDSYSEEISRKQRLLINMLGKLADESTAFNKNNNI
                     RLIASKPKSNFSLATRVSIFKHLAQIDYDMIITQFISGSFRSFEERRLLFPYYDGELA
                     RDVVSQTAKALGVEENEASQKTVVTIDILNGCGEKGLANTASILFESYGYKVVSVGNA
                     PSFDYAKTIMYDNDGDKAALQQVANIINCRNFGDASALPGLQKANITIILGKDFNGRY
                     CIGQ"
     gene            25996..26358
                     /gene="rsfS"
                     /locus_tag="PLS88_01155"
     CDS             25996..26358
                     /gene="rsfS"
                     /locus_tag="PLS88_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOU06000.1"
                     /translation="MDSKAFAYQSAEILAQHKALNVLVMDVHEIAGWADYFVLATSTS
                     STHMRGLQKHIEEFLDSEKIDLLNRPEVTDDQCWLLMDAQDVIINIMSAEAREFYDLE
                     SLWFNAPRFAVTFRGNGI"
     gene            complement(26450..26794)
                     /locus_tag="PLS88_01160"
     CDS             complement(26450..26794)
                     /locus_tag="PLS88_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06001.1"
                     /translation="MNHKITMTADTQEEFELITLTNGDSADTIYSLRPTVRFDGAYDD
                     EFDYDSDDDDEFEDEEDLEDEDDLEEDEEFEDEEDIDEDFDEDEDLDEDEELEDDDFE
                     DEDFDYDDDTDE"
     gene            26988..28019
                     /gene="ispE"
                     /locus_tag="PLS88_01165"
     CDS             26988..28019
                     /gene="ispE"
                     /locus_tag="PLS88_01165"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOU06002.1"
                     /translation="MYALEIKAFAKINIGLKIFQRRISGYHDIESIFQNVSLSDTILI
                     STGKKNNKERVTIEGDFGCPINLSSVYRAAIEFDKASDGIISQNGVYIQVKKGIPAQA
                     GLGAASADAAATLCGLNELFSMQFSKKELTSISIRIGSDVPFFLYGGAAIVRGRGELI
                     NLIDPRTDFGIVILLPSWSSSTQDAYRLLDVLRCNDISWIPPATEFRESESVHCVCSS
                     DEKLKQWYHGPIVNWYLENDFQPVMEKQHREYKEWSQFLMKNGAICTSLTGSGSAMYG
                     VFKSMREADTAAEECRTLLKEAEKSGLCTIASIFSVAPLARSMIVSYIQACNVDTRSD
                     KERSCHGSD"
     gene            28006..28290
                     /gene="spoVG"
                     /locus_tag="PLS88_01170"
     CDS             28006..28290
                     /gene="spoVG"
                     /locus_tag="PLS88_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOU06003.1"
                     /translation="MEVTDIRIRKVSAEGKLKAYVTVTFDECFVVHNVKIIEGKSGMF
                     IAMPSRRTKTGEYKDIAHPICPDFRSKLQEKILAAYNSNAPSDTSVPEFD"
     gene            28342..28413
                     /locus_tag="PLS88_01175"
     tRNA            28342..28413
                     /locus_tag="PLS88_01175"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28374..28376,aa:Gln,seq:ttg)
     gene            28446..29102
                     /locus_tag="PLS88_01180"
     CDS             28446..29102
                     /locus_tag="PLS88_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOU06004.1"
                     /translation="MEHIALRVSPRGKLTKGELRQERKKCRLPAQIYGKGIEPISVFL
                     EGKEFTNVAKRITESMIIDLDLEGKRFAALLKEIQKETISGDILHIDFNLLRHDRKIR
                     VRVPLHLNGTAKGVKDGGILEHSIHEIEVECDSDHLPEKIEVDITDLEANHALHLREI
                     ALPEGVKLITNPDTVFAIIKFAHGEPENPSEVEAEAVEEAVAPTEQESAETTSEAVHK
                     "
     gene            29246..30718
                     /gene="tilS"
                     /locus_tag="PLS88_01185"
     CDS             29246..30718
                     /gene="tilS"
                     /locus_tag="PLS88_01185"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOU06005.1"
                     /translation="MKTLEYKVKKVLLDMHVDFHKPLAIAFSGGLDSSVLLSILVRII
                     GHENIRAIHVCHNIRPKEELEKEKIILQKTCRDMNIPLTIVNIRQGAISEYAHRTKCG
                     VEAAARQYRYHALIRTAQRFGISTIATAHQADDQAETFFLRLLRGGRLEALGGILQSR
                     LVDSTKDIYIIRPLLSFRRYELEAFARENSLEWSEDSTNRDNSFLRNRIRHELIPLLD
                     TRFPSWRSSIESYQLQAQELKSLVTSIARQRMPTMVRGRGKDAILDQTLFKKEEALVR
                     REILRLFLMQLGLEDIVNQGVLRSLEHAILKRAPRIEAGRCQFDLSCGVVKYIGKADS
                     SSAFLDRSISHLKDLWKLDEFFLKVREPGQYECGPFRIVVSQSKYDPSSNIAAIDKED
                     FIYLSIPLSFPFVFRNRKEGETISHGTFSKDLDAIFKKQKISSESKHIITILEDEYGI
                     AAILLMTFDGKLNIKSYYPPQLLCEKRKIMYILLSMKGDF"
     gene            30723..32606
                     /gene="ftsH"
                     /locus_tag="PLS88_01190"
     CDS             30723..32606
                     /gene="ftsH"
                     /locus_tag="PLS88_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOU06006.1"
                     /translation="MPDQNDNRNGNKPDLPIAPKGNRAALVLFFSIIVLLAVFLFFGQ
                     SENSREISYSSFLSYLELGEVKSIKIVDQRDIEGVLKGKGGAEMPFTTRIPYFDAELM
                     AKLEEKGVSVTGAISGTSPFQILFELTPWIFGFIMIWIMMKQVQGNNKAFSFGKSRAK
                     MYSDSNKKITFEDVAGQKEAKYELMEVVDFLKNPNKFLKMGAKIPKGVLLVGMPGTGK
                     TLIAKATAGEANVPFFHMSGSDFVEMFVGVGASRVRDLFEQGRKHAPCIIFIDELDAV
                     GRTRGSGLGGGHDEREQTLNQMLVEMDGFDTKDGVIVLAATNRPDVLDPALLRPGRFD
                     RQVVVAMPDVNERTEILKIHMRQIPTSGDVDVEKLSRATPGTSGADLANLVNEAALFA
                     IRKNKEAVEMDDFEDARDKILMGVARKSLVIADEEKRATAIHESGHALLHYFLAHADS
                     LHKVTIVPRGRALGLALSLPDKDTYSRTYGWLYDRIVISYGGYAAEKIIYGETTTGAA
                     QDIKQATEIAQKMVREWGMAKDIGPISLGEDEEPIFLGREIAQHKDYSEATAQKIDAA
                     IQGILDSALEKATNILKREKDRLVKLADELMQVETLEDKEVRELLGLPEGKLLLEEGI
                     APC"
     gene            32615..34540
                     /locus_tag="PLS88_01195"
     CDS             32615..34540
                     /locus_tag="PLS88_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOU06007.1"
                     /translation="MKTRQAAILVLFLSLSVAIIQSSCSSMPKNELNINSSEEAIIAR
                     LDLLVDTDPEQCIAVISNLFEQQREKNQSFEASIIGEKGFVLFSKAIDNINILFRKAV
                     ADQDILAARRLSFSASSLVQYVSFYDSLRKMVSEELSIIASPAMRLQLALWEAENKFK
                     EYGYVTGKAVLSMAYLGEQGEDSFIKDTAFSIEDLKVLIDARDPKEYFATWATRAKEN
                     NDLNAEAWFESLSSGRIDFSPAIQTSSQDWLADAVSSVVTVYVDRGFKVQGGYSIPDR
                     VLGTAFQISEGLYLTNYHVVQSEIDPTYKGYSRISIRPSDNPQIRVPAKVLSLDEELD
                     LALIKSTEYARCSILLPPPGMRFHAGDRVFAVGSPIGLENSITAGVVSSLSRKIISFG
                     EAAQIDVPVNQGNSGSPLFSENGVLVGMVFAGLPSFQNINFALPVQWISLSLPSLFEG
                     KEVVHARLGLIMGKGSGQSPLVLADLDPSLSAFRAGDILKSINGKVSKDIASIQFQLA
                     SVPKGALCFLEAQRDFQPVRRLRQISSTDGASLIPAWDRIHKNSILEGLFGARLIQLK
                     DSEKVGGLYSVQWVCPAEAADEIGLSENDVITVYKFRLDPKNKVFILEFSAKSRLSGY
                     FERTIHLEFSSDSATII"
     gene            34598..36517
                     /locus_tag="PLS88_01200"
     CDS             34598..36517
                     /locus_tag="PLS88_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU06008.1"
                     /translation="MPKIAILESDILIALDIAKSIEQEGLAEADIYEKIEQAYQDIDG
                     LEYELFIIDIGDNQPEIIDAAIRIHRQTGLYCLVVSDHGVTSISAKLREAQPLGILVK
                     PFSKRELIANVETALYHSSMEKKLRDRERRYRDLFAYSLSARCITDIEGSILERNRVF
                     ESSFSNPIKAENVKDILPSDMIWREIIESLEENNILQREIQTNRIIDSLEQRSRDFIC
                     SFSKFEEEGSSAQILCEFLDVTESRRLREELFQSQKREEIGRLTSGVAHDLNNFLTSI
                     MGFLEMIKLEIPNNSPAHEDIRGIDRVVKRASGLTRQLLGFSKHKPYSPAPINLKEVM
                     GDTYKILKRLIPENISLTLSLPEESLVVNMDISHLEQILLNLVVNARDALEKTENPHI
                     SIQLSQFGNDAFYPKEAMMQPGDYALIAVKDNGAGIDNQNIERVFEPFFTTKAKNQGT
                     GLGLSIVKSLVEMNGGYIKLESEKGRGTTIKIWFPILMGTDTTTKPMAQEEYSLLSMI
                     NPPNNILKGRHILVVDDDESILEACTRILESAGAIVDTCINAGEAILKAERVKFDLLL
                     TDIVLPGVYGTELWERMKKNSQAKACIFMTGYESYQTEQFAGVTLLHKPFDARTLLEC
                     CNNVLNSSIESQETI"
     gene            complement(36482..37216)
                     /locus_tag="PLS88_01205"
     CDS             complement(36482..37216)
                     /locus_tag="PLS88_01205"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06009.1"
                     /translation="MNIKIENLKKSYGSLEVLEDVTFSLEESCITLIIGPSGCGKTTI
                     LNIISGISQADSGSIIGIEGRKFSYCFQEPRLLGWLSAEDNLRFALGSLLQSSSRICD
                     IELRIERFLQEAGLWEFRKFKPSQLSGGMQKRLALARAFAFPADMLLLDEAFSEVDLR
                     KKIELMEDFLHLWKDEKPTVVIVTHDIHDALYLADKVIVLSQRPARVEGTIQIDIPHE
                     KRTFTSSELYGYEMELYRLLGFDRTI"
     gene            complement(37219..38016)
                     /locus_tag="PLS88_01210"
     CDS             complement(37219..38016)
                     /locus_tag="PLS88_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU06010.1"
                     /translation="MHKLGERRFMLLRWQLLGIISFLLIWEIGAILVKSDLILPSPAR
                     VATALYMLSGKNTFWLSIGGSFLRVLEAFILSTLIGLATGIISGIKPQARAFLNPLVV
                     AMRATPVMALILVAMFWFPAWQVPVFSAILMAFPVMHTSTEAGVHAVDQKLLQMSNFF
                     HVPSSLVFWRLRLPSALPYILSGAKNALGLSWKVVVAGEVLSQPRMALGTGMQEARLS
                     IETTTVFAWAIITILLCGLSEYLFGLLADGLSVRFRQSSQEENQDLG"
     gene            complement(38000..38965)
                     /locus_tag="PLS88_01215"
     CDS             complement(38000..38965)
                     /locus_tag="PLS88_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU06011.1"
                     /translation="MKRSMIFLVALFLLLASISILYALPKDKIAVSVIRGPSGLSSVW
                     MMSELSRTSPNEFEFISVASADMVVAKLLNGEIDAGVLPVNVAAKLYNAGAPIRALAV
                     VGNGMVKFLTTDPKLQSLTDCKGKTIYIAGQKATPDYIFQFLCAQKGLQIERDYTAIY
                     SLAYPEIAAGLASGKISYAVLPEPFATQAILKNPAIIAPIDITKEWQIATNQTDYPMS
                     LFVARKELIELHPDKARILLDSYRASVQKAIEDPQMIAKLAENLDLGISAQVAIIAIP
                     NSNFVFIEASQSIQSIEALLSVFLKFDPTSVGGVLPSRSFYAQIR"
     gene            complement(38962..40407)
                     /locus_tag="PLS88_01220"
     CDS             complement(38962..40407)
                     /locus_tag="PLS88_01220"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HOU06012.1"
                     /translation="MSLNCEEIDLILSEAPLVGTKIQNIYQPTYDSLILELFGKGTLT
                     YYFFSIAQNACRLHPLSTPAPKNERPLRFMECLRSRIRGGTILYASQIGKDRIAKIDI
                     IRTNEEGAPEQSYLYARLWSGAGNILLVSAEGIIIDALRRLPARNEVSGSTFILPQQL
                     DSNKQYSKHYVVRDLEGDGTFWKRIEQFYAQNADNLSRSILLAQAHDRYERKKLSIET
                     RLNHLVSKQNEYEQAERFRQLGDILLAGYYTEIQGKKAFARAYDYYDNKEILIEINQN
                     LNPAKNAGIYYEKYRKAKSSLEEIRSEIQHWQKSLNQLDTWLHRLENETNPLIIAKIL
                     RKGGTARSKEKPAFPCQYLDFRGWRILIGRSGKENEDILRHFVRGSDLWLHARDYAGS
                     YVFIKAKKNKSIPPEVIQSAAKLAIYYSKARKNGGGDVHTTFVKNLRKVKDGPIGLVI
                     PYLEKNLYITFTEAEVREIFQEYQTKEENLI"
     gene            complement(40465..43236)
                     /gene="secA"
                     /locus_tag="PLS88_01225"
     CDS             complement(40465..43236)
                     /gene="secA"
                     /locus_tag="PLS88_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOU06013.1"
                     /translation="MARNTILSALLGSKEEKDIKAVLPLLHKINELESWALSLDDSDF
                     PALTIKFKDRLAKGETLDDLLPEAFALVREAARRKLGERPFDVQIIGGIALHQGKIVE
                     MKTGEGKTLSSVTAAYLNALAGQGVHIITVNDYLAERDSQWMGQIYKFLGLTVGCILS
                     SMDNIARKVAYSCDITYGTNNEFGFDYLRDNMVWSLDQKVQRGHYYCIIDEIDSILID
                     EARTPLIISGPADDDTYKVNEVNRLASSLLEVKKNPETGEYPDEAKGELLEGDYKIDE
                     KSKRVMFTSEGLNHIELLLQKRGLIKESLFDPENFQFVHYFTQAVRAQHLFSKDVDYV
                     VQDNQVQIVDEFTGRILHGRRYSDGLHEAIEAKERIKIARRNRTLATITFQNYFRLYK
                     KLAGMTGTAETEAEEFNKIYNLDVMVVPTNRPVVRKDENDLVYLNEAEKYDAIIEAIR
                     EMHTKGQPILVGTVSIEKSEKLSSLLIRNGIKHEVLNAKNHAREALIIAEAGSKGAVT
                     IATNMAGRGTDIKLGGNPEFRARRKAGTEAPEEKYREILSREYETWTKDYEEVKSLGG
                     LCVLGTERHEARRIDNQLRGRSGRQGDPGRSVFFISLDDDLMRLFGGDNFKQLMGRAG
                     MKPGEPIYHPLLSKSIESAQKKVEERNFEIRKHLLEYDDVLNKQRNFIYEQRNSILAD
                     DNLIERVRDAAYEMLEDQNAILTQEFKDNPNKAIQEFSLWLFDSFGIEMTAKELTALY
                     KSDELIPAIRNKLELDLDSKIQAAGAESLNFFIRFSYLQEIDEKWLNHLESMEALREA
                     VYLRSYAQKNPLLEYKIEGSDIFESLIFSIRHNIASKVFRVRIKESTSTVEATSPRSA
                     SRIEATQTSHEAVSSFANESQSVRKESAIAAASAPATATVVRQGKKIGRNDPCPCGSG
                     KKYKYCHGR"
     gene            43292..44446
                     /locus_tag="PLS88_01230"
     CDS             43292..44446
                     /locus_tag="PLS88_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06014.1"
                     /translation="MKRFVSILCLLVVVADFLISCHSSVEEGKIQALFDLGYGRSDSS
                     IDISIQNKNSVDICLRKGIFHLLSRGEAKILRISSYGQTLAMWYDPKKGSAPLVLKDV
                     QMSDFSSEEDLGRFAIQVSFNDPGYIATDSRQMLYVADAQVVRRFDSDGRELPFLGQE
                     GIGGIRFPVITALDVLSNDDIAVSCASESEKAVYFYDRNGTFLQVLKWDGQHMPVPVS
                     VAENHPNASGSRIIASLESFLPCFLNGKRVVILKINYYSEKYDPSSGVTISIEPIGGW
                     IMVTDASSGAFLNSFPLQSSLEDLGIDQQMIAAQSNDIFCIRWRDVGLAGEVFRFNQQ
                     GRINGKLDFQVPDDGASLIAMIIGDDGNLYMLTQLSSKLRMYTWKILTLR"
     gene            44558..45487
                     /locus_tag="PLS88_01235"
     CDS             44558..45487
                     /locus_tag="PLS88_01235"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOU06015.1"
                     /translation="MNLEAFVLGCGGMMPLPYRHLTSMLIRREGELFLFDAGEGTQVS
                     IRRLNLRWKKITAIFISHMHADHVTGLPGILMLSSQVDREEPLYIFGPPKIAEFVDQN
                     RHLLDMYINYEIIVREIYEAGTIWQGDDYSIRAFPLKHTKMCFGYALEESKRPGVFYP
                     EKALALGVPRGPLWSELQSGKTVRLTEGSSIKPSEVMGEPRSGRKFSFVTDTLYFPEI
                     AQEVSNSDLLICEGMFEHALIESAVDKRHMTAHQAALIARDAGNIKKLGLIHYSPRYA
                     DRELKILLDEAREIFPDTVLTKDRMRFPIEFVD"
     gene            complement(45504..46871)
                     /locus_tag="PLS88_01240"
     CDS             complement(45504..46871)
                     /locus_tag="PLS88_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06016.1"
                     /translation="MKRTLRIILFIGTFVLLALIVGYVAWKIGEIRLLGEQSASAEYS
                     ILRNAASLIASEEDLGDQFVRDRLKSLYNASTHLLAVQVLDRNGLVLWKMPDESPYFA
                     SPATNPGSVFHAPGMSTIIYMTPLPDGMKLMALYSILSQRDISNILIVPAIILSAWIV
                     LLVVLQLVLKEKPKEPEEEIVKEELTETIEKQEYQENPLESASYSEEALSATQENIKN
                     PAVQETIENIEEEAKAEAAAEEEEEEREEEIAQKEVRAPENIQAAQSESEAIPKPLSS
                     SDDFLPGEFVAPAPQTDEETEEVPLPEVFPSTLSLAELKRNLDIELQRDQETSLLLIH
                     CILSGELDPSALALGVTIRDYFASESLVFELGKGCYAAVLPGTDAGSALKVAVDLDDV
                     LTTTASLYKDLSEEPPFYFGISARYDRVIFPDRLYREAFAALEEAQERSGSRILAFKP
                     ASAIA"
     gene            46991..47842
                     /gene="amrB"
                     /locus_tag="PLS88_01245"
     CDS             46991..47842
                     /gene="amrB"
                     /locus_tag="PLS88_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOU06017.1"
                     /translation="MATSRRIRKRYMSSGWYPEDSAEISSLVADWTQSQKNLSAYAVL
                     SPHAGWYFSGDLAAKSVWALRDCDTVAILGGHLSSGDPILYGDEDDFDCTVRDAENDI
                     EMLNALKVELKNVGIQEIASDRDIDNSVEIILPLAGLRFPEARILWLRVPPDYKAKEL
                     GAALFRAASSCKKQLVTIASSDLTHYGPNYGFMPRGLGELAVQWVRNENDRGFINAAL
                     EMDADKVLKHARNNFSACSSGAVAAAISFALNAGAKHSLLIEHKLSFDIHPDRSFVGY
                     ASIAFVP"
     gene            complement(47847..49304)
                     /gene="murD"
                     /locus_tag="PLS88_01250"
     CDS             complement(47847..49304)
                     /gene="murD"
                     /locus_tag="PLS88_01250"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOU06018.1"
                     /translation="MDEFRGKHITIMGLGLHGGGIASARFFAKAGADVTVTDLRGAET
                     LSPAIYELKGLPIRYVLGRHEKKDFIDADIVIKNPAVRRDSPYLAFAKRIETDISIFL
                     RYSKSPLIAVTGSKGKSTIASALWHVLLKNNKKALLGGNITVSPLDFLDETDTEVPVI
                     LELSSWQLGDLRGRNLLKPLVAVLSDILPDHLNYYDSMETYVSDKRVIYESQDDNCWT
                     VCNADQKWGHSFAEETRARVLWYSESPLEESVGKRALGGWLIKSKTKLSNPDNFGAFS
                     SSVDLLEGRGKFNFDGPEELLVPSQVLVPGRHQKKNLLAAAVAARAFGLPSEDIMSGL
                     ASFPGVPHRLEFVGSTRGIRWYNDSTATIPDAAIAAIESFSSPIVLIAGGSDKMSDFS
                     AFAQKARSLKALILLAGSGTDRLIPILDANKVAYKGPYFSMHEAVQTADKQADPGDIV
                     ILSPGCASFGMFMHEFERGDSFKKEALMLFRQYAE"
     gene            49528..51684
                     /locus_tag="PLS88_01255"
     CDS             49528..51684
                     /locus_tag="PLS88_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU06019.1"
                     /translation="MNRIEHHPILSAKKNLTLVNFSFDGEEISGYEGEPVASALISEG
                     RHVFSYHFRDGFPQGLFCANGQCSQCTMLIDGIPKKSCVTPLAKGMDVRTIHGLPRLP
                     SQDSPLVQAERRSIRTDILIIGAGPAGLAAAAELGSMGFQVLVADDKPQAGGKLILQT
                     HKFFGSEADCYAGTRGIDIANILQEEALQYPSVTILTNAPVVGIYKDRKAGIYLNYES
                     YLLVEFTALVVAAGAREKALHFPGWDLPGVIAAGAFQTLVNRDLVKAYMKILIIGSGN
                     VGLIAAYHALQADIEVAGIFEAAGRVNGYQVHADKIRRLGIPIHLNTTITRAEGCQKV
                     QRAYVAAVDERFQPIPGTTRDYEVDSVLIAVGLAPCNEFLEQARRYGIMAVAAGDASE
                     IAEASSAIYGGKTAAANLALMLGLGKKGAIDPGWEKTRKVLASRPGDCYERSPIVPGK
                     EWRPVFFCSEEIPCNPCATVCPLRSIELRSMQGNIMDLPYFAGDRCTGCGACVAICPG
                     LAITLVRRVADDKAQVVLPWEFNVDSGLEEDMELVDQKGEVLERVPIISKRKVQKRNT
                     WLLTFEVDVAHAARIAGVRHLKTQQVPRPQKDTIEFKNIPSIISVEKSSLEDLDTVVC
                     RCERITLREIVDFIKKNDVKDANQLKSLRVGMGACGGKTCSQLIAKAFIIAGVNPAEI
                     EPFTQRPLFMEVPMGSLVNEGLHKLGGKDAGTRGAP"
BASE COUNT        14956 a        10406 c        11805 g        14685 t
ORIGIN      
        1 aacccacgtc tccagcttgg aaggctgggg taatagccgt tatacgacgg tcgcaatcaa
       61 tttgtgcctc tttccgcaag caaaggcaaa gctttctaag tgtcctattg tttttgcctg
      121 cttgtagctt ctcgacttgg gcgatttaag cgcacgatgc cttgcttgtc aagtaggcct
      181 aaggtaccca tccgccagag tggcttagaa gcgcccttaa aaatgaatca tacctaaatt
      241 ggctcttgct ccaaactgtc tttcagtata aaattgtgtc taatttgtaa gcatattgcc
      301 gcaatatttt atattttttc gatattggga agaggacgac ttatttttgc tgtttttctg
      361 agcttatttt tgttcgataa tcatgcacta atgagatgcg atcttgtttc ccaagcatcg
      421 actcaatgca gagggaatta agatggagat ccaattacaa gagcttcttg agagaattca
      481 tagcgaaggc atcgaaaatg caaagcagca agccgaagag attatccaaa aagctcattc
      541 ggaagcggca gaaattgttg cccgcgccag aagagatgca gaggagatgc agtctgaggc
      601 cctaaagcgc atagatgcaa tggaagcggc ttcgcgcgaa agccttcttc aggcttctag
      661 ggatacaatg atcgccttgc gtcaaagtgt tcaacgcttt ctcgattcag ccatcagggc
      721 tgatgtcaag aaagctttcg atgagaaaat ggccgcccaa gtgatacctg aagttctaaa
      781 gatactctcg tccggtcaat ctggcgactt cgaagtgctt cttccaccgg agcaattcga
      841 aaaaatagac agttctcttt ctgcaaggct tgccaaagaa ctctcgaggg gtgtcagctt
      901 taagccttac gatgccatcg atgcgggatt ccgagttgct ctgtctggtt cctcggttca
      961 atatgacttt tctgcagaga gcattgcaca gatgctctcg gttcgcgtaa ataagctcct
     1021 ctcccaatat ctaaaagaag ctgcggggag cctcgattga aatgaagcat tattggtact
     1081 ttgcttcgac acttacatct tttccatttg gctcaccccc tcccgtttct gtgcaggctt
     1141 ttgacgaact ctgctctagg atgattgata agagcgattt ggaaattttt ccttgcatag
     1201 aagaggcaag aaatggcaga ttttcagaat ccatgaagaa atctgcattc ttgatgtcat
     1261 attttgaatt tgaaagaggg cttcggaatg cgcttgtagt tttccggaca agagcttcaa
     1321 agcaagatgc tagccaatgg atgaggcctg gcgatataaa cccagattcg ctaaaagccg
     1381 cgcaggccgc acaggccgct tcggaccctc tccaagccga gtttgccata gagcgcgaac
     1441 gatggaatgc tgtggagaga ctctccgctc tttcagcatt tgagcttgac tatattcttg
     1501 catataaaat gaaattgctt attgcatctc gatatgcctc tttcgataga aatcgcggga
     1561 tgcaaggatt caatatacta taccaagata ttgtaaatgc tgcagctgca gtagatgcag
     1621 cgagtgcttt tgataccgga gtagcaacat gacagaagga tttgtcaccg cagtaaatgg
     1681 caacatggtt tctatacaaa cagaaggtat tgtaaccatg aatgaagtcg cttatatcct
     1741 ggtaggagaa agaaggctaa aaagcgaggt aattcgtatc cgcggtacct ccgttcaggc
     1801 ccaggtctat gagattacca aaggcataag agttggaaat agggtagagt ttagcaatga
     1861 gctattgtct gtcgagcttg gccctggcct tcttgggcag atatatgatg gacttcaaaa
     1921 ccctctgcca gagattgcca ataaggtagg gtattttctg gagccaggtc tttatctcag
     1981 ggcactccct cgagatataa agtggcattt taccccactc ttaagaaagg gcgatgtctt
     2041 aaagcgtgga atgggtatag gctgggttcc ggagcgatat ttccggcata tcataatgat
     2101 gccttttgat ttatatggag aatggaaagt tatagagatt gcaccagaag gtgattacac
     2161 gatcgatgag gcgatagcgg aagttgccga ttcgaaagga aacaagagac atatccgaat
     2221 gtgtttttcc tggcctgtga agagggctgt ggattgctat tccgaacgtc ttaagccttc
     2281 agagccgctt gttacgaaaa ctcgaatcat agatacattt ttcccagtgg caaagggtgg
     2341 aacttactgt atccctgggc ctttcggagc aggaaagaca gtcctgcagc aggtaacaag
     2401 tcgccatgcc gaagtcgaca ttgtgctaat agcagcatgt ggcgagcgag caggcgaggt
     2461 agtagaaact ctcaaagagt tcccccagat catcgatcca aagactgggc gctctttgat
     2521 ggaacggact gttattatat gcaacacttc gtctatgcct gtcgctgcac gtgaagcgtc
     2581 ggtatatacg gctgtgacta ttgcagaata ctaccggcaa atgggcctcg atgtgcttct
     2641 ccttgctgat tctacaagcc gttgggcgca ggcgatgcgt gagatgtccg gacgccttga
     2701 agagatccct ggagaagagg cttttccggc atatctcgag tctgttatag ctgcattcta
     2761 tgagcgcgct ggtatcgtaa ggctttataa tggcaaggag gggtctctca ctattggggg
     2821 aacggtgtcg cccgctggtg gcaacttcga agaacctgta acgcaagcta cgcttaaagt
     2881 tgttggtgcc tttcatggac tctcacgaga gcggtcggac gcacgcaaat atccttcaat
     2941 ccatcctcta gattcctggt ccaaatatga cggtatcatg cccaaatcga ccgttcgata
     3001 cgcccattca tttttagagc gaggagccga aataagcgca atgatgaaag ttgtaggtga
     3061 agaaggaaca agcttagagg attatgtaat ctatctaaag agcgaatttt tagatgcggt
     3121 ttacttacag cagaattcct tcgatcctgt cgatgcggcg gtatctccag caagacaaag
     3181 gcatgtgttt aaactgatac ttaagatact tgcagccgat ataagattct ccgacaaaga
     3241 agaggcgcga aattttttct atgagcttcg tcaaacaatg ctcgatttga atggtactcc
     3301 gcaagatagc gatatgttta acgctctata caatagagta gacgcagctc tggtcgatag
     3361 agaaccagag tttgataacc gggggcttaa attgatgagc acgctggagt aaggcatgaa
     3421 taagatatac tcgagaatag aatcaatcgt aggtaacgtt attgcggtag aagcgcagga
     3481 tattgcttat ggtgagctgg ctcaggtaaa tactagatat ggtgcttctc ttgcagagac
     3541 gatccgtcta gatggcaaga tggtgtatct tcaagtcttt tcgggtagtc gtggtatttc
     3601 aacaggcgat gaggtgcgtt tcttgggaca cccaatgcaa gtgagctttt ccgacgacct
     3661 tatgggcaga attttcgatg gagcgggaga gccgcgcgat ggaggcccag atctaacgga
     3721 aaatcttata actatagccg gaccagcagt aaacccctat cagcgcatta ttccgcgcga
     3781 gatgataaga actggtattc cgatgataga tgtgttcaac acacttgtca aaagccagaa
     3841 acttcctatt ttttcagtct caggagagcc atataatcct cttcttgctc ggattgcaat
     3901 gcaggctgaa gtcgacctta tcatcctagg aggaatggga ctcaaatatg acgactatct
     3961 tttcttcaaa gatactcttg agcagggagg ttctttgtcg aaaacgatta tgttcgtgca
     4021 taccgcggcc gatcccatag tcgagtgtct catggtgccg gatatctcgc tggcaattgc
     4081 cgagcgcttt gctctcaaag gcaagaatgt tctcgttttg ctgagcgata tgaccaactt
     4141 tgccgatgct ttgaaggaaa tcgctataac ccaagaacaa gtaccttcga atcgcggtta
     4201 tcctggcgat ttatattcac agcttgctgc gcgatacgaa aaagccgtcg atttttcgga
     4261 tgctggctct attactattc ttgcggtcac tacaatgcca ggagatgatg tgacacatcc
     4321 tattccagac aacacaggct atataaccga aggccagttt tatcttcgtg atggacacat
     4381 cgagcctttc ggatctcttt ctcgactgaa acaattggtc aacgccaaga ccagggaaga
     4441 tcatcgctct attatggatg cgatgataaa actttttgct gcatataggg acaccctcga
     4501 gaagaaatcg atgggcttcc agatgagtgc ctgggatcgc aaactcctca aatatggaga
     4561 actctttgag cagaggatta tggatttgtc tgtgaatatt cctctagaaa aggcccttga
     4621 tctatgctgg gaaacattgg ctgaatgctt tgagccacga gaaacaggcc ttcgttcaga
     4681 tcttatccag aaattttgga taggaaagaa ataattcgag ttagcaagaa gagaaggcaa
     4741 tagaatcatg ggaaagatca agctgaccaa aaatgagttg aaaaagaaga gagatgagct
     4801 caagatgtat gagcgctatc tcccaactct tcaactcaaa aaacagcagc ttcaagtcga
     4861 agtgcgcagc gtccagactc gcatggagga acttcaagca cagaaaacaa cgctcgagca
     4921 agaggcatca tcatggatgg cagtgttcac agaacaaact gctgcacgta ccaaggaagg
     4981 tgagcctatt ctgaaggtta gctccattag ctctgatact ggcaatatag ccggagtcgc
     5041 tattccagta ttccttggtg tagaattcga tcataagagt tatgatttat atgcgactcc
     5101 tctctgggtt gataatgcat tgattctttt ggagcggctt gttcgcacaa aaatagaact
     5161 cgatatagtc tcaaagcaga aagagctgct ttcacaagag ttgcgaatta caactcagcg
     5221 tgtaaaccta ttcgaaaaag tgaagattcc cgagactgtc gccgacataa gaaagattcg
     5281 catttacttg ggcgatcaag agacagctca agtagtgagg ggtaagatcg caaaaagaaa
     5341 ggtagaggga gccgaacgat gatagtacct atgaaaaagt tttacctcat cgtgctccag
     5401 agagatagag aaaaccttcc agagcgactt agaaatctcg gtgtagctca tgttgaggaa
     5461 cttcggggta taggagaaaa ataccaaggt ttggagagag aaaaggcaga ggctgaatcg
     5521 gtctattttc ttcttcagaa ttatgtcgac aaaaaatcca aaccaagcaa ggaagatact
     5581 tctatatctt taaaatatga agaatacaaa gatgtcagag atatcattcg cgaaatctcc
     5641 gcagctaagc aaagactgga tttgttagaa gatcgttcta tagacctaaa aagagaaata
     5701 aatagagtgg aaagctgggg agaagtctcg atggcaatgc ttgagcaaat catcgatggc
     5761 gtaggtttaa ctcttagatt cttcgaagct ccgctgaaag agctcgctac gctgccaaaa
     5821 gagaccgaat atataaggat ttctgctcca aaaggcagat gcaggatagg ccttattctc
     5881 cacgaagggg aagaaacacc agaattgccg ccatctttct tagagttttt accaccagag
     5941 attcctcttt cgcagatgcg acaagagatc gcttctatcg atagccaaaa gcaacagatt
     6001 cttagagaat ttagggaaaa ttcagcaaac ttatctctta taaagaaata tttgcataag
     6061 cttaattcta gcataatctt ggaacgattg cgatctggca tgccacagaa agaaagtctt
     6121 gcctatatga agggatatgt tcctgctcgc gactgcgaaa aattcaagaa ggctgccgca
     6181 aaatatggtt gggctattgc cttcgatgat ccgatagaag aagaccagcc tccaactctt
     6241 gtggaaaacc ccccagcagt tcgaataatc cagcctgtat tcgagtttct tggaaccgtg
     6301 ccgaattatt gggagtatga catatcgagc tggtttttga tctttttctc tattttcttt
     6361 gcaatgatat tcggagatgc cagctatgga gctatcatcg ttctagccac aattgcttcg
     6421 atcattgctg gaaaaagaaa aggaaagccg attggcgatg cacagaagtt atttcttctc
     6481 ttgggtagta caactgttat ttggggtgca cttactgcca catggttcgg tatagccttt
     6541 gatagtcttc caagactgct tcagaatatc tctattcctt caataaatgg tcagtctcca
     6601 gattccgaga ataatataaa ggttatttgt ttttcaattg gccttattca gctttcgatt
     6661 gctcacctaa agaatcttaa gcgtgatttc cccgacctca agtttctctc tcaggctgga
     6721 tcactactat tgcttcttgg catgttcaat gctgcattaa atttggtcat cgatgcaact
     6781 agatttccaa taacgaattg ggcgattgtc tgtatcgctg taggcttttt ccttgtattt
     6841 atctttggca attggaatgg caagcttggt tcgagtcttt tagaaagctt aaagaatata
     6901 attccaactt ttttaggcac agtaagtgtc tttgccgata ttgtctctta tataagactt
     6961 tgggccgtag gacttgcggg gctcgcactt gctcagacgg tgaatggaat ggcgggagga
     7021 cttttgagtg gaccctcaaa atttatcata gatcttattc tcaaattcat cattgcagca
     7081 gtgatcttgg ttgctgccca tgcgctcaat ttcttgctta cggttctttc ggttatagtg
     7141 catggcataa gactcaatat gctggagttt tccgggcatc tcggcatgga gtggtccggt
     7201 tacaaatacg atccgctgaa agaggatgag gatctttcgg cgtcatagca atctacccta
     7261 taggagagtg gtgtatgaat attgcattga taggtgctgc atgtgtgctg ggctttggtg
     7321 ccattggttc tggcatcggg gcaggaattg ctggcatggc cgcgattggt tcctggaaac
     7381 gatcttattt aaataataaa gcaccatcat ttttgctcgt tgcctttgct ggagctcctt
     7441 tgactcagac tatatatagc ttcatcttga tgaccagaat catcaactca acaaaagaac
     7501 cgttgctttt gcttgcagct ggcattatgt ctggactctc agaaggaatc tccgcgatcg
     7561 cacaaggaca ggctgcagct gctggctgcg atgcatttgg agagactggc aagggttttg
     7621 caaattacat cattgttgtg ggcttagttg aaactgtagc attatttact ttggctttca
     7681 cttttagcgt catttgatta agcacttagg aataggagct aagttttctt aatgaaagag
     7741 acgcacatta taaaagtggg ggacctgttt ttaggaggcg attggcctgt cagcatccag
     7801 accatgtgga aagatgcgct tcctagattt tcatcgtcag gggatgcaca gctgttggac
     7861 atccttgaga aactttcttc cctcaaaaag cttggctgtg acattatccg ctttgcagtg
     7921 ccagaaatat ccactgcaga atcgctcggg atgcttgcag gtctttctcc attgccaatc
     7981 gtggctgata ttcatttcga ttggcgaata gcgcttagat gcatggactt tcctatagcg
     8041 aagatacgta tcaatccagg caatatcggt gccgaatgga aggttcgcga ggtcgtagct
     8101 aaggccaaag acaaaggaat tcccattcgt ataggtatca atgccggttc attacctaga
     8161 gatcttgcaa tggaacaaga tgtcgtaagc gcggccataa aagcggcgga gcgcgaaatt
     8221 gagcttctcg aatcaatgag tttcaacgat atcgtagtat cgctaaagat gaaccagcca
     8281 gacgaagtcg tgcgagccaa tgaagagttt tccttacgct atccttaccc tttgcatcta
     8341 ggcgtcactg aggccggtcc tcttgtggca ggaatagtcc gcaataccgc agcgctcgtg
     8401 ccacttctaa aaaaaggtat tggagctacc atccgtgttt ctctctccga taccatggag
     8461 caggaagtaa ttgctggcaa agaaatcctt gcttgcgtag ggaaaggcaa gcatggaatt
     8521 aggattgtat cctgccctcg ctgtggaaga gcatctttcg atactcatgc cttcgtttct
     8581 agatggatga atcgcctgta ttcgctaaac gcagatatat cgattgccgt tatgggctgc
     8641 atagtaaatg gcccaggtga agcgcgacaa gcggctctag gcattacagg agcaggcaat
     8701 tcggtgttga ttttcaaaca cggagaaatt ataaggcgag ttgaaaaaga gcaagctgat
     8761 atcgcattcg aagaagaact cgaatcgtta ttgaaagacg aaatcaattc caactgagca
     8821 ccaatgctgt tgtaggccaa agctgtgctg tatatgctat tttcttcgag tcattcgctt
     8881 ttttctgcca ttttttctga ggaagaattc gattctgctt tgatttgaca gatcatcgca
     8941 tcgattagat tcttgataaa tggatcagag atagaataga tgcgacgcgt tttctttact
     9001 tcggaagtaa cgattccata gtgttttaga attgcgaggt gctgagatac tactggctgt
     9061 ggttgatcga gacagcgcca gatatcacca acacaaggta cttcttgatc ggcaatgagg
     9121 caaagaatct tcaaacgaat aggatgtccg attgccttga acttttgtgc ataaggctca
     9181 agtttttctt catagcatgt ttttgtggtc tggttcataa tgtctatatt atatagcaat
     9241 tattataaaa actcaagttg ataaagctga taatgcaaat tgcatccaga ataggctagc
     9301 tagatttctg ggttctgccg attttattca aagtttcatc gtacgctgtg cttttgcaac
     9361 tagcctgctg ccagtcttag aatcgatatg gtgggttttt agtatttcat ttgtaataat
     9421 agagggttct cttaggaatt gttcttcggc gatacagata catgggggga aatcaggaat
     9481 gcctctccca aggacaatca ttttgcagcc tttcagcggt gcataaaagc ggactagcgc
     9541 tgcaagcaac tgttgatgta aggggaaatc ttttcctcga atgatgactg cctcaggatt
     9601 aagctcagta agatggtcta atactcgcag aggatcgcga taagctatag aggcaatatc
     9661 cttatgattt aattgcctaa ggacaacgtt ggcaatcgat ttttcttcga taagaactaa
     9721 tactaacatg gtatcgtaat tttagcaccc atagataaaa ctcgctaaaa aggctatact
     9781 cctaactatc ttcaatgaca cagcatacga tgaaaaaaga caatctgcaa gcgctattcg
     9841 aaaagatatc taaaaatcca gcattgggaa aaatccaaaa gctagtagag caggactcct
     9901 ttcctattca tattcagaat tcggagaatt ccttgcttgc tctgtctctt gcttctttag
     9961 taaggcggac caaaagaaaa tgccttgtag ttcttccctc agatcatgaa acagaagagt
    10021 tttctcagga tctcgcactt acaggtgttg atcttctaga ttttccctta tggtttggag
    10081 ccccttatag ggcacttcct ccacgttctc gcatgtttag agaaagggcc tctgcacttg
    10141 caagacttgc agaaggcgat tttgaaattg ctactgtttc agcgcgcact cttgcgattc
    10201 cagttccacc acctgaatac actaggaatc atatcctgcc attgagaatt ggggcagatt
    10261 tcgaacccat cgccatatcg agcaaactgg cggaacttgg atacttgagg gtgccttctg
    10321 tcagcctacc aggcgaatat gccgtgcgag gtgaagtgct cgatatctat atgcctggat
    10381 atgaatacgc ggtccgtatc cattttgact atgatcgtat cgaaagaata gtccgcttcg
    10441 atcctgagac ccaaacaggg cgtgagcaga ttactcaaat ccatgtgaga cctctaaaag
    10501 aactcttatg gggtaaggct gagattgctc agcttcagaa taatgcttca gatttcgtgc
    10561 ggactgatcc tcgtattagg cagattattg agaatcttga cagtggaaaa gaaatgctag
    10621 gcgaagaact ttggtttccg cttgcatttg acaggataaa taacctaatg gattatctcg
    10681 acaataaggc aatgcttgtg ctagtagcca gcgaacgcat cgatgcaatt tccgaaacgc
    10741 taaggaaaga atatgcctca atgtatcgga ttgcacttcg ggaaatgctt gtgccaccgc
    10801 cgcataagat tcatataaac ctagatggac tatctgctgt tacctcgtct gtgctgtact
    10861 gccatgcatt gaaaggaatc gatggctctg tagagcgaat atctttggaa gcagagccac
    10921 aacactctta ttttgggaat ataaggctct tcaaagaaga gttgtctggt ttcaaaaaag
    10981 aagactataa gacatggata attgcttcta ctgagccaca ggcagaacga attagttcac
    11041 tcattcagga tgattctatc attatatcta caggacctat ttctgctggt tttactatac
    11101 catcgctcag attgcgagta ctcgccgagc atgagctttt tggcaggcgg aaacatgtgc
    11161 ctcgctctat tgccaagacg aagagctctg taatcgaaag ctttatagaa cttacgccag
    11221 gcgattatgt agttcataca aactatggca taggcaagtt cataggtata gagcgcctaa
    11281 tagtccttgg tctcgagcga gattacattt gcatcgagta tgcagccgat gaaaaagtct
    11341 tcgtgcctat cgaacaagct aacctcgtgc aacgatatat cggcaatgaa ggggaggctc
    11401 cgcatctcga ttcggttgga tcaaaggttt gggaaaacag aaaaaaaaga gtcagcaaat
    11461 cggtggagga gctagcagaa cggcttatca ggatttacgc tcgtagaaaa tccgcgcatg
    11521 gctttgcgtt tccttccgat aacgaatggc agatccaatt tgaagcaaca ttcccatacg
    11581 aagaaaccga agatcagcta aaatgtattg aagaggtaaa gcacgatatg gagagcccaa
    11641 agcctatgga tagattagta tgcggcgatg tgggctttgg taagacagag attgcaatgc
    11701 gggcatgttt caaggccatc acagcaggca aacaggttgc attcctggct cctaccacga
    11761 ttttagcaga acagcatttc gagaactttc tcgagcgaat tggagatttc ccaataaaag
    11821 tagcattgct ttcacgattg gtcgatagaa aaacacagaa ttctattatc aagggattgg
    11881 ctgatggaag catcgacatg gtaattggaa ctcataggat tctgcaaaat gatgtccatt
    11941 tcaaggatct agggctgctt gtcgtcgacg aggaacagcg ctttggggta aaagataagg
    12001 agcggctaaa ggagatgaaa gtcggaatag actgtctcac tttgacagct actccaattc
    12061 ctcgtaccct tcatatgtcg ctcctcaaga ttagagacat gtctgttcta cagacgcctc
    12121 cgctggagag gcagccaatt cagacttttg tagaggaatt ttctcctgaa ctcgtcgctc
    12181 gtgcaataag aaatgaaata gctagaggtg gtcagatatt ttatctccac aatcgcatag
    12241 agacgctacc ggaggtagag tactttatcc gacaactcgt ccctgaagct ctagtagaga
    12301 gtgcccacgg aaagctcgat ggcaaagatt tggaatcgat aatgcatcgc tttattcatg
    12361 gagcattcca tgtccttgtt tctactacta tcatagagaa tggcatcgat ataccaaatg
    12421 tcaacactat aattattgat agatcggata gttatggtat ttctcagctt tatcaactga
    12481 aaggcagggt aggaagatcc gatagacaag cctatgccta tcttctctac ccagataaaa
    12541 gggctctcag cgaaattgcg atgaaacgac ttcagataat atccgatttt actgagcttg
    12601 gctctggctt caaaattgcc atgaaggacc tagaagtgcg tggagcgggg aatcttctag
    12661 gacgcgagca atcgggaaat atctatgcgg ttggcttcga tttatatcta aaattgctag
    12721 atgaggcggt atctaggctt tctggcattg cggaagagga agaagaaccc tatcttgaac
    12781 tcgagtattc aggttttata ccagacagct atatctcggt tccaatgatc aaaatggaaa
    12841 tctataagcg tattgcctct attaggacac aagaagagat agattctctc catgaggaac
    12901 ttgaggaacg ctttggtcca ctcccagacg aggctctctc tctgctttcg cttgcggaaa
    12961 tgagactgct atgcagaaga ctctctatag ggcggctgca agagcgggca ggaatcgtta
    13021 ccgtagagtt ttcaaaggtt tcgaagattt ctgttgagaa gattctgaga ctcatacgtg
    13081 aatctgcagg cacaatccgt ctatcaccag aaaaaccaaa tgctattcag attcagacaa
    13141 gagcaattgg cctaaaggag aaaagtgcct atctcaggga gcgtctttcc cttctagccg
    13201 gttgaagcag cttcttgcca agattcatgg gtaagccaaa gcagaacaca gctggttgtt
    13261 ctggcaagat aaaggcttcc ttccgccaag aagcaacaga catcgacctt attagaggct
    13321 gattggttcc tagatcatat gcaacgcaga gatagatatt ctcaggcagt gcttcaatac
    13381 aagccttcaa tgtctttgca ttacgatatg gagtctcgat aaagattctt gctgcttcat
    13441 cgtgcatggc tgctttgcct tcacgagcca gctctttttt aagctcttcg ctttggattg
    13501 gcatgtagcc aagaaaacag aatcgttggc cattgagacc agaagcagca agggctagga
    13561 gcaaggagct atcgcctcca attgggacaa cccgcaaccg ttcctcatgt gctagataca
    13621 caagcgctgc gcctggatcg gctatacaag gaagccctgc ctcagaaagg atggcgcagt
    13681 cataaccgct tttcagtgca tcaattgggg ctttaagatc ctgagcttcc gagtgttcgc
    13741 ttaaaataaa aaaatggacc ttagagaatt gctcaggcga aagcaatctt gatagaagac
    13801 gctcagctgt tttctgtgat tccactacaa aatatggaac ctttctcatt aaagtaagaa
    13861 catttggagc tatcatattc atgagctggc aaggcatcca attttcaggt gaataaggcc
    13921 ataaaggggc tggtataaga taaagggtac tgctgctttt ttcagtctct gacatgatat
    13981 ctctcattta gtctttagct ataatatact tgatcgttga aggttatcga gtgcttggga
    14041 tattaatggt acgaaaagct ctgtattcca aaagttagta cgaagaagac tttcgctata
    14101 cattctaagg gcagactgaa aaacgcgttc tgtcaaagga tagggagaag agacaatttt
    14161 ctgttcagca tcatagtctc ggtatgcacg ctgtccagct ataactaact taaagttaga
    14221 aatcgagttt cgttcgatgg aacatgcaga aaccaagtat gcgccatctc catccgggaa
    14281 aatccctcca ccagtcctcc taataaaaag gtaatcgata gattgaagcg ggattcgaat
    14341 tcttgtaagt attcttctcg actccagagt cggcaagccc tcttcattag tcaggtacca
    14401 gatatttttg atgcttgttc ctgatggccc tttgaactcc agttccgtat ctaggcatgc
    14461 caatagaggc catagagcaa ggaatctctt ttgatacata agatgcccgc caatcgttgc
    14521 aatgttgcgt accgcggagt ttgccgttgc tcgcaggact tctctggcag gattcttttc
    14581 gaagggatat acggattcga gctcgcttag agaacatgct gccccgaaag atatcatacg
    14641 ttcagtttta tggatggttt ttagttcagg tacatttctg atacacgcga tagaaggctc
    14701 gaaagataga tagcgtggtt cttgaatata tccgaagctt gttgcgccag ctactatctg
    14761 tatattcttt tgtctctcca tgatgactat gagttcttga acactatgcg ggtagtagat
    14821 ttcgctaagg ttcatggcaa ttttctcgct atagccaatt gttgtattaa ttttaatagt
    14881 gtattagcaa aggctctagg atcaatacat tggcaatgca agctagccag gatgtctgat
    14941 atttcataca acgaaggagt caaggatcta tggaccaact ccattgctac aagcatcagc
    15001 gaattgttgc agtaagtgca ggacaataga ccttcctttt cgagctctgc agatagaagg
    15061 tcatattcct tgctcttagc aaaatattcg attgtttcta catgctgttc ccgcagaagg
    15121 aaggcaggca gaatatagct gcatacaggt ttgccatcat atagaataag gcaattgctg
    15181 caatccctat caggactgat ctcagagaaa ctttttatgc cacattgatt aataagcaca
    15241 tcgaaagctc tctcgacgct agtgattgtg attctctgca aattgccatt caaagtaaat
    15301 tcaatgttca tcatctttct ccaaaagctc tgtagaggat atagcagtag gggtaggcct
    15361 aagaggcaat ataagccttt ccgagctatt caattgctgc atcacctcga gaaagcatga
    15421 tagtacaaga gtatatgcga gatctcctat agggagcgta gaatttaccg tttcatcttt
    15481 cacaagttct atattgattc tgctttcgga aattatccat tcatatatat catttatatc
    15541 tatatccgga taaaagcttc gtctaataca tgaaaggaga gcctcgatac ttgcagagcg
    15601 aattgcgcct tttgtcttga acgggcagag gattttaccg gcataaatcg atatatctag
    15661 acgaatattg cggaagatcg atctgaggct atcataatcg acctcgagga ttgcagctcc
    15721 ttgcgctaga tcggtaatca tcgaaatgga agacgagtcg atatggctct gatcagcgat
    15781 agagagagaa atagctcgta ttctagcttc tcttgcccca cgggttcttt tatgaatttg
    15841 ctcgtatgct gcgtattttc tatgaaaatc cgtatgttcc aataatggtt ttacaatttt
    15901 ttccgtagaa gattcatttt tggcaggcat cttggttata gctgaatacg aatctctttt
    15961 acccaaggta atacacaata attctagaaa ctctaaatcc atttttcttg cagcacgagc
    16021 aatatgagtc tcgagcatgc taaatatcgc tgacgaagaa atacagtcta tggctcccat
    16081 aggaatcgtg ttggttctta ccgcaaagcc aataattgtt aaaggaatgt taggtatagc
    16141 atcggcaaca agatgcacag tcttttctaa tataagctca tctaatacag aataggctcc
    16201 aataggaatc gcaaagcggc attcaatatt cataaggtta tgtttgtatt gtgaccactt
    16261 tgttctcata tgaagagata taccatgcac acaaggaagg aatcgttttt ctcgttgaat
    16321 tgatataagg agctttacag gccttttcaa agcccgagca gctgctgcgc ttctggctcc
    16381 aagcaaagaa gggtaccaga aatatatgcc agaattgtca tacatcttag attgcaggat
    16441 ttcgatattc tcaatcgagg aaccaattag ctctgcaata catgaatgta aaagctcagg
    16501 ccatagtgta gggcatgcta gtttaagtct atcatcttgc cattctgcta aagcacctaa
    16561 gccgctatga aagctaaatt cacggggctc caaacgcaaa taggaagcca gttcaaagct
    16621 atcttcagcc cctgaggatt cgggggattc ggaagatacc attgcatttt ctttagaata
    16681 atcaattcgg gctgaaatat ctgaagatga aaatacctgc cagtctagtc tggattcctg
    16741 tccttcgcaa tcaactattg tcttcaatgc aagctcttcc gcaattagtg gatccggacc
    16801 tacgatgaga ccaactgcct ctcctatata ggagactttt tttgaggcta acaatggcag
    16861 gcttatgccc ttgattttta gctcatttct aaatggcaac tgatcagcgg tgataagagt
    16921 atagccctct ggcaaaggag ggactttgat gttttgaatc tctccaatat tcgaaagcgc
    16981 tcttatatta aaggcatata gcatgcctgg cgccgagagc tgttcaaatg gaatacactc
    17041 atttattgtc ataataaatg ccttgtggtg cagtttcggc agtatcagct tccatgactt
    17101 caaatgccaa atcgagaatt attacagatt ctgctatatc aagctttact tttgcacggc
    17161 gttttctctt attgaccttt ataataaagc cttcgatgcc ttgtaagggt ccctcgatta
    17221 cttttatttt ctgattttcg tcgaatctta caagcgatgg aggaatcaag cttccaaaat
    17281 ttatgaaatg gcgaataatt tcgctatcgc gattattaag tggttgcgga gcctcgttgt
    17341 ttggcaggaa tcgtatgaag aattgagaat gtcttaatct gtatatggtc tctagtggca
    17401 gagattctcc attatactcg aaaaatatat agccaggaaa aactggtgaa ttctcgagct
    17461 tggtttttcc tctttttctt atatacattt ttttcattat attatggaat tttagatttg
    17521 ttacctggtg ctgaattcgc tcaagccatt cgtcttcctt gcgtgttgtc acctggattg
    17581 cataaaaacg catgccaata gaatagcata tcctaaatat cgagtcagta ttatttagag
    17641 aaatggaata taattttacc taacagtaat ttgacaggct ttctttggat gattagtata
    17701 agagcatgcc gaatgattta aaagccaatg aagatataca ggttcgacag aagagaatca
    17761 gagaattgga gtctctgatt agacatcatc aggaactcta ctataatggt gagcccgaga
    17821 tctcagatga ggaattcgat gccttatggg atgaactggc ttcgatagat ccagagaatc
    17881 ctcttattgc ttctattgga gcggacagat cagatcgatg gcctaaatat aggcatagga
    17941 tgacaatggg aagtctctcc aaggctacgg atccagaatc atttctagcc tgggcagcca
    18001 aagtcaagta tccactttac cttgtacaat acaagcttga tggcgccagc atagagcttc
    18061 aatatgatga aggtgccttt gcaaggggtg tcactcgcgg agatggggtt ataggcgacg
    18121 atataacccc taatgcaatt cgtatgaatg gagtgcctaa gcagcttcaa gagccattta
    18181 caggatcagt gcgcggcgaa gtgctgatgt cgaaaagagt gcatgctatc aagtattcag
    18241 acaaggccaa ttgtcgcaac gctgcaaatg gtcttatgaa gcgtaaagat ggtcagggag
    18301 tcgaggatct cgacatcgtc tgttatgatg cagtgggtag attacctgca ggcgaagcct
    18361 tttccaccga acgcgaaaag attgtttggc tgaaaagaat gggcttcaat gttgttccaa
    18421 ccgtagagtg caagaatcct gaagagatca ttgtatatcg cgctaagatt atggaactaa
    18481 ggcctactct ggagtatgac atagatgggc tagtcataaa gggtgatcga atcgatcccg
    18541 aggatatgaa caaactgcgt cccgaattgc agatagcctt taaatttagt ccagaagagg
    18601 ctgttacaac gcttatagca gtggaatgga gcgaatctgg aactaatgtg acccccattg
    18661 gtatagtcga acctgtaagg cttgcaggta ctactgttca acgagccaat ttggctaacc
    18721 cgggcatgat taggtctatg aacctaaaaa ttggctcgaa ggttgtaata acgaaacgtg
    18781 gagagatcat tcctaagatt gaatccctgg ttgaaaatcc caaagatgct cttccgattt
    18841 ctattccttc gcattgctca caatgtggag cagagctcat agatgaggat acgcgtctat
    18901 attgtccaaa tcctgaatgc ccaggaaaag cctatcatcg ccttgagaaa tggctatccg
    18961 tagttgatat aaaagatatt ggatcgacct tgttaaggag gcttttcgaa gccaagagag
    19021 tgatgcgtat tcctgatcta tatacgctta cgatggatga gcttatctcc tttgagcgaa
    19081 tgggagagaa gagcgctcaa aaaatattgc gcaatataaa aggacgaaat gaagtcagtc
    19141 tttctcagtt tattgcgggc tttgatattg aaggaatcgg tttgcttatg gcagacaagc
    19201 ttgtcgaagc tggctatgat actctcgata agatcctagc tgcttcgcca gctgattttg
    19261 aaaaaattga aggttttgca gagataaccg caaaggctct ttacgaaggg ctgcacctag
    19321 tcgagaaaga tatgcgcgag ctcgtcgata gaggatatgt gcgaataaag cctccgctcc
    19381 gcagaattca aaggggtaca aatccaattg ccggtaagag cttttgtttc acgggagagc
    19441 tgaggtcgat gaagcgctcg gatgctgaaa aactagttcg agaaatgggc ggcattgcaa
    19501 aatctagcgt aactaaggat ctcgactatc tagtcacgaa tgatcccagc tcaggatccg
    19561 agaaaaacag aaaggctcaa gagctatcga tcaatatcat cgatgaaaaa gaattccttg
    19621 aaatgatcgg ccttaaatga tggatcttag atgaaagcac cttcaataag aactcaagaa
    19681 cacctagatt ttctcaagcc agctctccat gaagagaaag aaggggtagt gctcgaagtc
    19741 tatgtggtcc ctcgtgcgcc ttgcgatagt gtggaagaac tgcgcaatgg gcgcttattg
    19801 ctgagagtaa aagcagcgcc agaaaagggt agggcaaatg aggctgtatt agagttgatt
    19861 gcgaattatt tggatatggc gccctcaaat cttcgtatat tgcgaggcaa tagctccaga
    19921 cgcaagtcta tcttattgca aaactttaga tataaccgct aaaatggagc acctcatgga
    19981 atattatgta gatcaatgga aatcgataat tgcgaactgc ttaaatgagc tcgctttcca
    20041 aaaagaactc aaggatgcct atttatcctc tcaagatatc attgccgaga agccacctag
    20101 gccagagttt ggagatatag gcttcccaat gttctcatat gcaaagcaat ttcgaatgcc
    20161 accggccaga atagctgcag aggtagcgaa tgtctgcaat ataaaaaccg atactgagaa
    20221 gcctatatgg cctggtaccg taaaaacggc aggtccatat ataaatatat acctagacaa
    20281 aaaatatgta gcaaattatg tccttgaaaa cgcggagaaa gaattttggg agtctagtcc
    20341 actctttaat ggtcgaagga taatgattga gttttcttgc ccaaatacca acaaacccct
    20401 tcatcttggg catcttcgta acaatgtgct tggagagtct ctttctagaa tcatgaaaga
    20461 agcaggggca gaagtcaaaa aggtcaatct gataaacgac aggggaattc atatatgcaa
    20521 gtcgatgctt gcatatcttg cttatggaga aggacgaaca ccagaagatg agggattgaa
    20581 gagcgatcac tttgtaggca aatactatgt cttattcaat agactaaaag atgaagatcc
    20641 atccgcagaa gaaaaagctc aagaactttt gcagaaatgg gaagccggag acgcagatgt
    20701 aattgcccta tgggaaaaga tgaataattg ggctgttgaa ggcataatgg ctacctatag
    20761 acgccaaaag gttagtttcg atgaattcga ttttgaacat gagacctata agttaggcaa
    20821 agaggaagtg ttggaaggcc ttgctcgtgg gatattctat cgcgcagagg atggctcaat
    20881 tcaggtggat ctagaagata tcggtctagg gaaaaaagtg ctcctcagaa aagatggtac
    20941 aagtatctac attacgcagg atatcgggac agcaattatg cgctacaaca gatggccttt
    21001 ccatgagctc gtgtatgttg tggcttctga acagcagtac catttcaaag tgctttttga
    21061 agttctaagg aggcttggct ataattgggc agaaaatctc taccatcttt catatggact
    21121 ggttaatctg ccttctggca gaatgaaaac cagggaagga acagtagtag atgccgatga
    21181 tcttatcgac gagcttacaa gattagcagc aaatgaaata gcagataaga gaagagagga
    21241 agcagtaggc gatatcaaag atgttgcaga gcaggtagct cttggagcct tacattattt
    21301 tcttctacaa gtttcgccgg ctaaagacat gctttataat cccgagcaat ctatctcttt
    21361 cactggagat acagggccat acattcaata tatgggagct cgagctagtt ctattcttcg
    21421 aaaatataaa aatggtgagg gtaatgcaaa ccaagggaaa atcaatgttt ctcttatagt
    21481 atcggattcc gattggtctc ttatgaggct attgatagaa tttcctcata ctattgaaca
    21541 atcggcaaaa aacaaagatc cttccattct cgctgcatat acccatcagc ttgcgagcga
    21601 gttttctgtc tggtatagag acaatccagt gctcaacaac gacaatccag atatctcggc
    21661 tagccgctta gcgttcgtga tcgcaataag agaaacgctt agaagagcaa gtaatttgct
    21721 atgcataccg tttctcgatg ccatgtaaga aagcctgttt taagaagtcg cttttttaga
    21781 gttctgagag ctgttgacaa agtggggtta taaagtgtat aaatttcagc ccgatatgag
    21841 tatttgagca ttgatgagga taagctatat gtatgcaatc gtagaaatca acggaaaaca
    21901 atatcatgca gagcagggga aggatctagt agtggatcgc tttgatgcaa tgccaggtca
    21961 gtcgatcgtt ttcgaaaagg tattattgct tagtggagat gagaccaaag ttggtagtcc
    22021 gtatgtcaat gatgctgccg tcaaggctat agtaaaagaa gagatcaaag gtgataagat
    22081 aattgtattt aagtataagc caaaaaaggg gtatcacaaa acccgcgggc atcgtcagcc
    22141 ttataccgtg ctcacggtga atgaaattgt tggcgcctga ggcgtccatt ttaaccggag
    22201 gaatatagca atgccgcata aaggtgttaa tggccgagat tcaaatccac agtatcttgg
    22261 tgtaaaggcc tattcaagcg agaacgtaaa cgcgggctcg attattgtac gccagagggg
    22321 ttctagaata aatccaggca agaatgttgg taagggtaag gatgataccc tatttgcgct
    22381 tatcgatggt acggtgcgct tccgtgaata taaaggcaag aaatttgtag atgtggagcc
    22441 agccattcaa gcatgagcat aaaaaccaga gcataattct aaccgctcct gtagcacaga
    22501 gagcggtttt ttattatgct tatgagttat gggagtattt ttgtgatcaa attcgctgat
    22561 gaagcgataa taagcgtcgc aagcggtaag gggggcgatg gttgtgttgc atttaggcga
    22621 gaaaggttca ttccttttgg cggtcctgca ggcggtgatg gagggcgagg gggagatgta
    22681 atttttattg tcaagcagaa ccttcggacg ctcgcatatc ttcgatatcg taaggttttc
    22741 aaagctgaaa acggtcagcc gggcatggga aagaacatgc atgggagaga tggaaaggat
    22801 gtcataatcg aagtcccgcc agggacgatt cttcgagatg ccgagaccaa tgagattata
    22861 agggattttg gccgtcgggc tggtggcaaa agagctggaa cgctagagta tgtagaacca
    22921 tgggttttct taaaaggcgg caaggggggt tggggaaata ctcattttaa gagctcagta
    22981 aatcaaacac ctcgttacgc tcaacctggt cagccaggac aggagagaaa gctcaaagtc
    23041 gaactcagtc taattgcgga tatcggtttc gttgggtttc caaatgccgg taaatcaagt
    23101 ctgttagagt attttacaaa tgcacgtcct aagattgcgc catatccttt cacaactaag
    23161 atacctaatc tgggcgttct tacggtgaat gaaagggata ttattttagc tgatattccg
    23221 ggcataatag agggagcaca tgaaggagct ggattggggc ttcgtttcct aaaacatatc
    23281 gcaaggacag cttgccttgc ttttctagtg gatctttccg acgagcattg gcgaacagcc
    23341 ttcccaattc tgttgcatga attggggtta ttttctccaa ccctagtgca gaagccgcgt
    23401 atcttgctgg gaacaaagat ggatctaccg gaatcccaaa cgaattttat ggagttcaaa
    23461 gcatcttttc cagaagaaag tgtctatggt atatctgttt ttactgggca aggtctggat
    23521 gaggtcaaac agcaattctt tgaaaaggtt ctagcctatg aagtagccga ggcctatgaa
    23581 gctcaaaaag aaaatgcccc agatcgccta ttcgatgaaa tagatttttc ggatgagggc
    23641 tttatttgat gagaattgcg ataattggag gttccttcaa tccgccccat ataggacaca
    23701 tgattttgat tgaagagatt cttgccactg aatgttatca gagagtattg cttattccag
    23761 ctaaagctgc gccacataaa gttccgcaga tggacccagg tccggaaatg cgacttgctc
    23821 ttttggaagc aagcattgaa ggatggcaca atgtggcgat tgaagattgc gaattgaggc
    23881 gagctggtct atcctatact tcagatacct tggaagaact atggaataga agactgttcg
    23941 atgaaaggcc agggcttgtg ataggagatg atctagctac tgatttttta gcaacatgga
    24001 aacgggccga gaagattctg gaattagcgg atattatcgt tgctcatcgt ctctatgttg
    24061 acaaaatccc attgccttat cctcacaact atatcgaaaa tctcattatt ccaatctctt
    24121 ccacgcttgt tagagagagg attcatgctg gtggtgcatg gcgttcgcta gttatgcctt
    24181 ctgttcgcac aatcatagag aactatggac tatatcgaaa tacctgagat tgccaaagta
    24241 atagaaaaag agattgcatg tactttgact cccaaaagat acgctcactc tagatcaacc
    24301 gcagagaccg cggtaaaact cgccgagcgt tttggcgaag accgatgggc cgctcatcta
    24361 gcaggattat cgcatgatat gtgtaggggg ttgagttttg aggagcaaga taatctggtg
    24421 aatcgacata aagcttgtat agcttttctt agtaaacgcg catccctagc ggcaatccta
    24481 tcggataagg aatttaaaga aaaaatgatt catggcccag ctgcagcttg tttgctctgt
    24541 cataggtttc aggttaggca agaaccgata ttggaagctg tggcattgca tagcattgca
    24601 gatgatgcaa tgtcccctct tgctaagatt gtatacattg ccgataaact cgaaccgctt
    24661 cgcaatcggg cagccgatgc cgatgaaaaa atgcagactc tagaccttga ttcgcttttt
    24721 gcatatacgc ttacttcagt tgtaaaatgg ttctccgaat ctgggagacc gctttgccca
    24781 tacactgccg agatatatag caggatgcca aaattatgag cgactcgaaa tcaaaaattt
    24841 taaaacgaag ctctctacca cttttagtga ttctaatcgt tgtagcaata gggcttttct
    24901 ttgttgtcaa gctcacagag aagggaactg tcgagagcat tatcaagaaa gaccagccta
    24961 tggctatact cttcattttt gagagtgata aaaaacctgt ttctaatcaa cttttaattt
    25021 ggtatccgtc gaggcgcaag gctgccatta tggatattcc aagcactatg ggcatcatac
    25081 taaaaagtgc aaataaaatg agcagcatcg acactgtcta tgattctcga aatcccagta
    25141 aattcgtcaa agaaataagc gattatctta agtacccaat tgatggctgg tttgtctacg
    25201 atgaagtgag cctttgccag acaatagatc tgctggaagg aattcagatg tttatccccg
    25261 agacggtgat agagagtgac tcactaaaag gagtgtctct tcctggaggt gcagtagtgc
    25321 tagatgggga taagacaaac caataccttc tttatgcctt agccacagac tcctattccg
    25381 aggagatcag ccgcaaacaa aggttgctta taaacatgct gggaaaactt gctgatgagt
    25441 cgacagcctt caataagaat aataatattc gtttgatcgc atcaaaacca aaaagcaatt
    25501 tcagtcttgc aactcgcgtg agtattttta agcacttagc tcagattgat tatgatatga
    25561 taatcacaca attcatttct ggatctttta ggagctttga ggagcgtcgt cttctatttc
    25621 catattatga tggagagctt gctcgggatg tggtgagtca gacagcaaaa gcgctgggcg
    25681 tagaagaaaa tgaagcgtct cagaagacgg ttgtgacaat agacatacta aatggctgtg
    25741 gagaaaaagg gcttgcaaat acagcctcga ttctcttcga aagctatggc tataaagtcg
    25801 tatcggtagg caatgcacca agtttcgatt atgcaaagac aattatgtac gacaatgatg
    25861 gggataaggc ggcgcttcaa caggttgcaa atatcataaa ctgtaggaat tttggcgacg
    25921 cttcagcttt gcccgggctt caaaaagcca atatcactat aatattggga aaagatttca
    25981 atgggaggta ctgtattgga cagtaaggcg tttgcatatc agagtgctga gattctagcg
    26041 cagcataaag cgctcaatgt tctcgtaatg gatgttcatg agatagctgg atgggctgat
    26101 tattttgtac tagcgacaag cacaagttcc actcatatgc ggggacttca aaagcatatt
    26161 gaggaatttc tagattccga aaaaatagat cttctcaata ggcctgaagt taccgacgat
    26221 cagtgctggc tgctaatgga tgcccaggat gtgataataa atatcatgag cgccgaagct
    26281 agggagttct atgatctaga atcgctttgg ttcaatgcac ctcgctttgc agtcactttc
    26341 cgtggaaacg gaatctaaat ctggaatagt ctcaataatc ttaggtatga gcaaggtatt
    26401 gaaaaggcgc ggttttagcc gcgccttttc aatctataac accgttgtct tattcgtcag
    26461 tgtcatcatc ataatcaaaa tcttcgtctt cgaaatcatc gtcctcgagt tcctcatcct
    26521 catctaggtc ctcatcttcg tcgaaatctt catcgatatc ttcctcgtcc tcgaattcct
    26581 catcctcttc caagtcatct tcatcttcta gatcttcttc gtcttcaaac tcatcatcat
    26641 catcgctatc atagtcgaat tcatcatcgt aggcgccatc gaatcgcaca gtaggtctaa
    26701 gcgaatatat ggtatcggca gaatcgccgt tggtaagagt gatcaattcg aattcttcct
    26761 gcgtatcggc ggtcattgta attttatggt tcatagtgcg ctcctcccat aaatattctc
    26821 tttgcattga agcagatgta ctattacgag aaacggccag tgcgtcaata cagaggcatg
    26881 gcaaaattta taggtcaaat gaaaatatgt caaggaaaaa ttacaaaaaa catttctttt
    26941 tggagagctt ttaatcaaaa aagttatcat atataatata atcgataatg tacgcactcg
    27001 aaatcaaagc ttttgcaaaa atcaatatcg gactcaaaat ctttcagaga agaatctcag
    27061 gataccacga tatagaaagc atatttcaga atgtaagttt atctgatacc attttgatat
    27121 ccacgggcaa aaagaataat aaagagagag tgaccataga aggtgacttt ggatgcccta
    27181 taaacttatc aagtgtatat agagcagcga tcgaattcga taaagcttcc gatggaatta
    27241 ttagccaaaa tggtgtatat atccaagtta aaaaaggaat tcctgcacaa gcaggcttgg
    27301 gtgcagccag tgctgatgct gcggctacac tctgcggctt aaacgagctt tttagcatgc
    27361 agttctcaaa aaaagaacta accagcattt caataaggat cgggagcgat gttcctttct
    27421 ttttgtatgg aggtgcggct attgttaggg gacgaggcga gttgataaac ctaatagatc
    27481 ccagaaccga ttttggaata gtaattttac taccttcatg gtcttcttct actcaagatg
    27541 cttataggct tttagatgtt ctgcgctgca atgatatttc ttggattcca cccgcaactg
    27601 aatttcgcga aagcgaatcg gtccattgtg tctgctcttc ggatgagaag ttgaaacaat
    27661 ggtatcatgg tcctattgta aattggtatc tagagaacga ttttcagcca gtaatggaaa
    27721 aacaacatag agaatataaa gaatggtcac agtttcttat gaaaaatggc gcaatctgta
    27781 caagccttac tggttctgga tcggcaatgt atggagtatt caagtctatg agagaagctg
    27841 atacagctgc cgaagaatgc aggactttgc tcaaagaagc cgaaaaatca ggattgtgta
    27901 ctattgctag cattttttct gtagcaccgc ttgcgcgaag catgattgta agttatattc
    27961 aagcttgcaa cgtagataca aggtcagaca aggagcgttc ctgccatgga agtgactgac
    28021 atccgtatcc ggaaggtaag tgctgaagga aaactcaaag cctatgtaac agtgaccttc
    28081 gatgaatgct ttgttgttca caatgtaaaa ataatcgaag gtaaaagcgg catgttcatt
    28141 gctatgccta gtaggcggac aaaaacagga gaatacaagg atatagcaca tcctatttgt
    28201 cctgatttcc gatcgaaatt acaggaaaaa attctcgctg catataattc taatgcgcca
    28261 agcgatactt cagtgcctga atttgactga caccttcaca ttttttcaat ttcctcgtat
    28321 attgcaatga attcttttta ttgggacgta ggcaagcggt aagcctccgg attttggttc
    28381 cggcatgcgc aggttcgaat cctgccgtcc caggaaaagg catataggtc aaggagtaga
    28441 aaaatatgga acatattgca ttgagagtct ccccgagagg aaaacttact aaaggagagc
    28501 tcaggcaaga acgcaaaaaa tgtagattgc ccgctcagat atatggcaaa gggattgagc
    28561 caatctcggt ttttctcgaa ggcaaggaat ttaccaatgt tgcaaaacgt atcaccgaga
    28621 gtatgatcat cgatctagat ttggaaggca aaagatttgc tgctttattg aaggagatcc
    28681 aaaaagaaac aatttctggt gatatcctcc acatcgattt taatctcttg aggcatgata
    28741 gaaagattcg tgtcagagtg cctcttcacc tgaatggaac agccaaaggt gtcaaagatg
    28801 gaggaatttt ggaacattca attcatgaga ttgaagttga gtgcgattcc gatcatcttc
    28861 cagaaaagat cgaagtagat attacagatc ttgaagcgaa tcatgccctt cacctgagag
    28921 aaattgcttt acctgaagga gtaaagctaa tcactaaccc cgataccgtc tttgcgatca
    28981 taaagtttgc acacggagag cctgaaaatc catccgaggt cgaagctgaa gcagtcgaag
    29041 aagctgttgc gcctacagag caagaatctg cagaaacgac aagtgaagca gtgcataaat
    29101 aacttttcaa tatctcaatt ttgtcttaat agtccagcgt tccagcttaa gaggctggaa
    29161 tgctgggctt tttattacta ggcttgtata ttcataagac aagatgctag ggtacccaga
    29221 ggcaaaccca agcaaggcaa agagaatgaa aaccctcgaa tacaaagtca agaaagtcct
    29281 cctggatatg catgtggatt ttcataagcc attggcaatt gccttttcag gaggactcga
    29341 ctcaagtgtt cttctcagca ttctcgttcg tattattggg catgagaata taagagccat
    29401 ccatgtatgt cacaatattc gtccaaagga agagcttgaa aaagagaaga taattctgca
    29461 aaaaacatgc agagatatga atattcctct gaccatagtt aatattaggc aaggtgctat
    29521 ttctgagtat gcacatagga caaagtgtgg cgtcgaagca gcagcaaggc aatatcgata
    29581 tcatgctctt ataaggacag ctcaaagatt tggtatctcg acaatagcca ctgctcatca
    29641 agccgatgat caagctgaaa cctttttctt gcgtcttctt cgcggggggc gccttgaggc
    29701 tctcggtggt attttgcagt caagattagt cgatagtaca aaagacattt atattattcg
    29761 cccccttttg agttttagac gctacgagct agaagcattt gcaagagaaa atagcctcga
    29821 atggtcagag gattcgacaa atagagacaa ctcttttttg agaaatagaa ttagacatga
    29881 gctcatccct ttactcgaca caagatttcc ttcctggaga tcttcaatcg aatcttatca
    29941 gctgcaagct caggaattaa aatctcttgt tacttcaatc gctcgccagc gtatgccaac
    30001 aatggtgaga ggtcgcggca aggatgccat actggatcaa accttattta aaaaggaaga
    30061 agctttggtg cgcagggaga tcctaaggct ctttctcatg cagctagggt tagaagatat
    30121 tgtcaatcaa ggcgtgttgc gatcccttga gcatgcaatt ctaaaaaggg cacctagaat
    30181 cgaagcagga agatgtcaat tcgatctctc ttgtggagtt gtcaagtata tcggaaaagc
    30241 ggattcttct tctgcatttt tagatcgaag tatttcgcat ctaaaggatt tatggaagct
    30301 agatgaattc tttctaaaag taagagagcc gggtcaatac gaatgtgggc cattcagaat
    30361 cgtcgtatct caaagcaagt acgatccttc ttcaaatatt gctgcaatag acaaagagga
    30421 ttttatatac ctatcgattc cgctttcctt tccatttgta tttcgaaatc gaaaggaagg
    30481 tgaaactata agccatggaa ctttctcgaa agacttggat gcaattttta aaaagcagaa
    30541 gatttcttct gaatcaaagc atattattac tattctagaa gatgaatatg ggattgcggc
    30601 gattctttta atgacttttg atggcaaatt aaatataaaa tcatactatc ctccacagtt
    30661 gctgtgcgaa aagagaaaaa taatgtatat tttgctatcg atgaaaggag atttttagat
    30721 aaatgcctga tcaaaacgat aatagaaatg ggaataagcc cgatctgccc atcgctccca
    30781 aaggcaatag agcggctctt gtcctgtttt tttctataat tgtcctatta gcggtctttc
    30841 tattctttgg acaaagcgag aattcgcgag agatttctta ttcttcgttt ttgtcttatc
    30901 ttgaacttgg agaagtcaaa tccatcaaga tcgtcgatca gagggacata gagggagtac
    30961 taaagggcaa gggtggggca gaaatgcctt tcaccacgag aataccttat tttgacgctg
    31021 agctgatggc taagcttgaa gaaaagggag taagcgtaac cggagcgata agcggcacaa
    31081 gtcctttcca gatactgttc gaactcacgc catggatatt tggctttatc atgatatgga
    31141 taatgatgaa gcaagtgcag ggcaacaaca aggcgttcag ttttgggaaa agccgggcaa
    31201 aaatgtatag cgattccaat aaaaagatca cctttgaaga tgttgctggg caaaaggaag
    31261 ccaagtatga acttatggag gtagtcgatt ttctcaagaa tcccaataag ttcctaaaga
    31321 tgggcgctaa gatacccaaa ggagtccttc tagtgggtat gccggggaca ggcaagacgc
    31381 tcatagcaaa ggctacagct ggtgaagcaa atgtgccatt tttccatatg tcgggttcag
    31441 atttcgtcga aatgttcgtc ggtgttggtg ctagccgtgt tcgtgacctc tttgaacaag
    31501 gtcgaaagca tgctccttgt ataatcttca tagatgagct cgacgcggta ggcagaactc
    31561 gaggttctgg acttggggga ggacatgacg aaagagaaca gactttgaat caaatgcttg
    31621 ttgaaatgga cggatttgat acaaaggatg gagtcattgt tttggcagcc accaataggc
    31681 ccgatgttct agaccctgca ttgttgagac caggtcgctt cgatagacaa gttgtagttg
    31741 caatgcccga tgtaaatgag agaaccgaga ttcttaaaat acacatgcgc cagattccaa
    31801 cttctggtga tgtagatgta gagaaactat ctcgtgctac ccctggaact tctggagccg
    31861 atcttgctaa cctggttaac gaagccgctc tctttgctat ccgcaaaaac aaagaagcgg
    31921 ttgagatgga cgatttcgaa gatgcgcgag acaagatatt gatgggtgta gcacgaaaaa
    31981 gcttggtgat tgcggatgaa gaaaaacgtg ccaccgcgat ccatgaatcg gggcatgcac
    32041 ttctgcatta ttttcttgca catgcggatt cgcttcataa ggtgactatc gttcctcggg
    32101 gaagagctct gggcttggct ttatcattgc cagataagga tacatactca agaacttatg
    32161 gatggcttta cgatcgcatc gtgatttctt atggcggata tgcagcagaa aaaattattt
    32221 atggagagac cactacagga gcggcacagg atatcaagca ggccacagaa attgcccaaa
    32281 agatggtgcg cgagtgggga atggcgaagg atattggtcc aatctctctt ggagaggacg
    32341 aagagccaat ttttctagga cgtgaaatag cgcaacacaa ggattattca gaagctacag
    32401 cccaaaagat agatgcggcc atacaaggaa tccttgattc tgcattggaa aaggcaacca
    32461 atattctaaa gcgagaaaag gatcgactag ttaaacttgc agatgagctt atgcaagttg
    32521 aaaccctgga agataaagaa gtaagagagc ttctaggctt gcctgagggt aaactactct
    32581 tggaagaggg gatcgcacca tgttgaggaa ttgaatgaaa acacggcaag ctgctattct
    32641 tgttcttttt ttatccttaa gtgtggcaat aattcaaagc agctgttcat ccatgcccaa
    32701 gaatgagtta aatatcaatt cctctgaaga ggcaattatt gctcgtttgg atctgcttgt
    32761 agatactgat cctgaacaat gtatcgctgt tatctcaaat ctattcgaac agcaacgaga
    32821 aaaaaatcaa tcttttgagg ctagcataat cggcgagaaa ggatttgtgc tttttagtaa
    32881 agctatagat aatatcaata tcctctttag aaaagctgtt gcagaccagg acattcttgc
    32941 ggctcgtcgg ctctcttttt ctgcttcttc tttagttcaa tatgtatctt tttatgattc
    33001 tcttagaaaa atggtatcgg aagagctttc gattatagca tcgccagcaa tgagactgca
    33061 gcttgctctc tgggaagccg agaacaaatt caaagaatat ggttatgtta cagggaaggc
    33121 agtgctatcg atggcttacc tgggagagca gggagaagat tctttcatta aagatacggc
    33181 tttttctata gaagacctga aagttcttat agacgcaaga gatccaaaag aatattttgc
    33241 tacttgggct accagggcta aagagaataa tgatctcaat gcggaagcct ggtttgaatc
    33301 gctctcgtct ggtcggatcg atttctctcc ggctatccag acttccagtc aagattggtt
    33361 ggcagatgcg gtatcgagcg tggtcacagt atatgtggac cgcggattca aggttcaagg
    33421 cggatacagc attccggatc gtgtgcttgg cacagcattt caaatttctg aaggattata
    33481 cctaacaaac tatcatgttg ttcagagcga gattgatcct acatacaaag gatattcgcg
    33541 aatcagtatt aggccttccg ataatcctca gataagagta cctgcaaaag tccttagctt
    33601 ggatgaagaa ttggatttgg cgcttattaa gtccacagaa tatgctcgtt gtagtatttt
    33661 gctgcctcct ccaggaatga gattccatgc aggagatcgt gtctttgccg tcggctcgcc
    33721 tataggtctt gaaaatagta ttacggctgg tgttgtcagt tcattgagca gaaaaatcat
    33781 ttcctttggc gaagcggctc agatcgatgt tccagtcaat caaggtaatt caggaagccc
    33841 gctcttttct gaaaatggag tgttagtagg catggtcttt gctggattgc catcatttca
    33901 gaatataaac ttcgcactgc ccgtccaatg gatttcattg tctcttccct ccctattcga
    33961 gggaaaggaa gttgtccatg ccagacttgg acttattatg gggaaaggca gtggtcagag
    34021 tccgctagtt cttgctgacc tcgatccgtc tctttctgca tttagagcag gtgatattct
    34081 taaatctata aatggaaagg tttcaaagga catagcttcg attcaatttc aactcgcttc
    34141 tgttccgaaa ggcgcactat gttttttgga agcgcagcgc gactttcaac ctgtcaggag
    34201 attgagacaa ataagctcta ccgatggtgc ctccttgata cctgcatggg acagaattca
    34261 caagaattca atacttgagg gacttttcgg ggctaggctt attcagctta aggactcgga
    34321 aaaagtaggg ggcctctatt ctgtacaatg ggtatgccct gcagaggcag ccgatgaaat
    34381 aggccttagt gaaaacgatg ttataactgt ttacaagttt cggctcgatc caaaaaataa
    34441 ggtatttatc cttgaatttt ctgccaagtc taggcttagc ggatatttcg aacgcactat
    34501 ccatttggag ttttccagcg atagtgctac aattatttaa aagcttcttc catagacaat
    34561 cgattcatag caagagggaa tcaaggaggc ctgcaagatg ccaaaaattg ccattcttga
    34621 atccgacatt ctaatagcgt tagatatcgc aaagagtata gaacaagaag ggctggcaga
    34681 ggcagatatc tatgaaaaaa ttgagcaagc atatcaggat attgatggac tcgagtatga
    34741 gctttttatt attgatattg gagataatca acctgagata attgacgcag caatacggat
    34801 acatagacag actggcctat actgtctggt tgtaagtgac catggcgtta caagtatttc
    34861 agcaaaacta cgagaagcgc agcctcttgg cattcttgta aaaccatttt ctaaacgaga
    34921 gcttatcgcc aatgtcgaaa ctgcgctcta ccattcatcg atggaaaaaa aattgagaga
    34981 tagagagcga cgatatagag atctttttgc ttatagtcta tcagcgagat gcatcacaga
    35041 tatcgaagga agtattctag agagaaatag agtatttgaa tctagctttt ccaatccaat
    35101 aaaagccgaa aatgtaaagg atatcttacc cagcgatatg atctggagag agataatcga
    35161 aagccttgaa gaaaacaata ttctgcagag agaaattcaa acaaatagaa ttatagatag
    35221 cctcgaacaa agatcgcgcg atttcatctg cagcttttct aaatttgagg aggaaggaag
    35281 cagtgcacaa attctatgtg aattcttgga tgtcaccgaa tctaggaggc tgagggagga
    35341 actatttcag tcacaaaaga gagaggagat aggaaggcta acaagtggtg ttgctcatga
    35401 tctaaataat tttctgactt caataatggg atttcttgaa atgattaagc tcgaaattcc
    35461 taataattcg ccagcacatg aagatatccg aggaatagat agagttgtta agagggcttc
    35521 tggacttacg cgacaactat tgggcttttc caagcacaag ccttattcac ccgctccaat
    35581 caatctaaaa gaagttatgg gcgacactta taaaatatta aagagactta tccctgagaa
    35641 cataagtctt accctaagcc ttcctgaaga gtcattagtt gtaaacatgg atatatcaca
    35701 tctcgaacag atacttttga atcttgttgt gaatgccaga gatgcacttg aaaagactga
    35761 gaatcctcat atatcgattc agcttagcca atttgggaac gatgcgtttt atcctaaaga
    35821 agccatgatg cagccaggcg attatgctct aatcgcagtt aaagataatg gagctggtat
    35881 agataatcag aatatagaga gagtattcga gccttttttt actactaaag caaaaaatca
    35941 agggacaggt ctaggattgt ctattgttaa atctctcgtc gaaatgaatg gcggctacat
    36001 taagcttgag agcgaaaagg gaagagggac tacgatcaag atctggtttc caatccttat
    36061 gggaaccgat actacgacca agcccatggc tcaagaagaa tactctctgc tctctatgat
    36121 caatccgcct aataatatat taaaaggaag gcatattctt gttgttgacg atgatgaatc
    36181 aatattggag gcctgcacaa gaatcctaga gagtgcagga gctattgttg atacttgtat
    36241 caatgctgga gaagctattc ttaaagctga aagggtcaaa ttcgatcttc tgctcacaga
    36301 tatagtactg ccgggcgtat atggaactga attatgggaa aggatgaaaa aaaatagtca
    36361 agcgaaagca tgtattttca tgaccggata tgagtcctat cagactgagc aattcgcagg
    36421 tgttacttta ctgcacaagc cattcgatgc tcgtactctt ctcgaatgtt gtaataatgt
    36481 tttaaatagt tcgatcgaat cccaagagac gatatagctc catttcatat ccatagagtt
    36541 ctgaagaagt aaaagtcctt ttttcgtgag gtatatcgat ctgtatcgtc ccttccaccc
    36601 gggcagggcg ctggctcaat acgatcactt tatcagctag atataatgca tcgtgaatgt
    36661 cgtgtgttac aatgacaaca gtcggctttt catctttcca taaatggaga aaatcctcca
    36721 taagttcgat tttttttctc agatcaacct ccgagaaggc ctcgtctaaa agaagcatat
    36781 cagctgggaa agcaaaagcc cttgccaaag caagcctttt ttgcattcct cccgataatt
    36841 ggctaggctt gaatttccta aattcccaaa gtccggcttc ttgtaaaaag cgctcgattc
    36901 tcaattctat atcgcatatt ctagaagagg attgcaaaag agagcccaag gcaaatcgca
    36961 agttatcttc tgcgcttagc caaccgagaa gccttggctc ctgaaagcaa taggagaatt
    37021 tacggccttc aatgccaata atagaaccgc tatcggcttg ggaaatccca gatattatat
    37081 ttaagatagt cgtcttgccg caaccgctgg gccctataat taaggttata caagattctt
    37141 cgagagaaaa ggttacatct tcgagcactt cgagagatcc atagcttttt tttagatttt
    37201 ctatcttgat attcatcgtc agcctagatc ctgattttct tcttgagaag actgtctaaa
    37261 tcgtacactt aatccatctg ctagaagtcc gaataaatac tcgcttagac cacaaaggag
    37321 tattgtaatt attgcccagg caaaaaccgt agtcgtttca atagaaagtc ttgcttcctg
    37381 cataccagta cccaacgcca tacgtggctg actcaaaact tctccggcga cgactacttt
    37441 ccacgagagc ccaagagcat tttttgctcc agataggata tatggaaggg cagatggcaa
    37501 acgaagacgc caaaaaacca atgatgaagg aacatggaaa aaattcgaca tctgaagaag
    37561 cttttgatcc accgcatgca cacctgcttc tgtcgaagta tgcattacag gaaatgccat
    37621 aagaattgcg ctaaaaacag gtacctgcca agccggaaac cagaacatag ccactaatat
    37681 caacgccata acaggtgttg ctcgcattgc tacaactaat ggatttaaaa aagctcgagc
    37741 ttgaggcttt attccgctta ttatgccagt agcaaggcct ataagagtgc taagaatgaa
    37801 tgcctcgagc accctaagga aagaaccacc tattgaaagc caaaatgtat tctttccaga
    37861 aagcatataa agggctgtag caactctagc aggacttggc agaattagat cgctttttac
    37921 aagaatagct cctatttccc atattaagag aaacgaaata atccccaata gctgccatct
    37981 aagcagcata aatcggcgtt cacctaattt gtgcatagaa tgatcggcta ggcaacacac
    38041 cgccaacgct tgtaggatcg aatttcaaga atactgatag taaagcctca atgctttgga
    38101 ttgattgaga agcttcaata aatacaaaat tagagtttgg gatggcaata attgccactt
    38161 gagcgctaat gcctagatct aaattctctg caagctttgc tatcatctgc ggatcctcga
    38221 tagctttttg gacagaagca cgatacgaat caagcaatat tcttgcttta tctggatgta
    38281 attcaattag ttcttttctg gcgacaaaga gagacatggg ataatccgtc tgatttgtag
    38341 caatctgcca ttcctttgtg atgtctatgg gtgctattat ggctggattt ttaagaattg
    38401 cttgagttgc aaatggttct ggcaatacag cataagatat ctttccgcta gcaagccctg
    38461 ctgctatctc aggatatgct aggctataga ttgctgtgta atctctttct atttgcaaac
    38521 ctttttgggc gcataagaat tggaagatat aatcaggggt ggctttttgt cctgcaatgt
    38581 agatcgtctt acctttgcag tcagtaaggc tttgtaattt ggggtctgtt gtcaagaatt
    38641 tgaccatacc attgcccact actgccaaag ctctgatggg cgccccagca ttgtagagct
    38701 tagctgctac atttacaggt aaaacaccag cgtctatttc tccatttaga agttttgcga
    38761 ctaccatgtc agcgctggca accgatatga actcaaattc attagggctt gttctggaaa
    38821 gttcagacat catccataca ctgctcaatc cagaagggcc tctaatgacc gatactgcaa
    38881 tcttatcttt aggcaatgca tatagaatag aaatactagc cagtagcaaa aataaggcaa
    38941 ccagaaagat catagatcgc ttcatattaa gttctcctct ttagtttgat attcttgaaa
    39001 aatctccctt acttcagcct ctgtaaaagt aatatataaa tttttctcta aatagggaat
    39061 aaccaagcct attggtccat cttttacctt tcgtagattt ttgacaaaag tcgtatgaac
    39121 atcaccacca ccattttttc gagcttttga gtagtatatt gcaagctttg ctgcagattg
    39181 gattacttct ggggggatcg atttgttttt cttagctttg ataaatacat aagagccggc
    39241 ataatcccgt gcatgcagcc ataagtctga accccgcaca aaatgccgaa ggatatcttc
    39301 gttttctttg cctgagcgac cgatcaaaat tctccagcct ctaaaatcaa gatattgaca
    39361 aggaaaagca ggcttctctt tcgaacgagc ggttccgcct tttcgtagga tttttgctat
    39421 gattaaggga ttagtctcat tctcaagtct atggagccaa gtgtccaact gattaaggct
    39481 tttttgccaa tgctgaattt cggaacgaat ctcttcgagg ctagatttag cctttcggta
    39541 tttttcgtaa tagatgcctg catttttggc aggattgaga ttttgattta tctcgataag
    39601 aatctcttta ttatcataat aatcataggc acgcgcaaat gcttttttcc cttgtatctc
    39661 agtgtaatat ccggctagca aaatatctcc caattggcgg aatcgttcgg cttgttcata
    39721 ctcgttttgt ttagatacaa ggtgattcaa tctcgtctcg attgatagct tttttctttc
    39781 atatctgtcg tgcgcttgag ctagcaagat agagcgagaa agattgtcgg cattttgggc
    39841 ataaaattgt tctatacgtt tccagaatgt accatctcct tcgaggtcgc gaactacata
    39901 atgcttagaa tattgcttat tgctatctag ttgctgaggc aatataaagg ttgatccaga
    39961 tacttcattc cgagctggaa ggcgtcgtag tgcatcgatg attatgccct cggcagagac
    40021 taagaggata tttcctgctc cgctccataa ccttgcatat agataggatt gttccggggc
    40081 cccttcttca ttagttctga taatatcgat ttttgcaatg cgatctttgc caatctgtga
    40141 tgcatataat atggtgcctc ctctaattct tgagcgaaga cattccataa atcgcaaagg
    40201 gcgctcgttt tttggagctg gagtgcttaa tggatggagc ctgcaagcat tttgggcgat
    40261 gctaaaaaaa taataagtca atgtaccttt gccaaaaagt tccaatataa gagaatcata
    40321 agttggttga tagatatttt gaatttttgt accgacaagc ggagcttcgc ttagaatgag
    40381 atctatttct tcacaattta gtgacataga gacactccaa acattctaga aagcagccaa
    40441 tatcatttcc gatatcaaga ggttctatcg gccatggcaa tatttatatt tcttgcccga
    40501 accgcatggg catggatcat tgcgacctat tttctttcct tgtcttacaa ctgtagcagt
    40561 agctggtgcg gatgccgcag ctatagcaga ctcttttcgt actgattgac tttcgttagc
    40621 aaaagaactt actgcctcgt gacttgtctg tgttgcttct atcctcgatg ctgatcgcgg
    40681 acttgttgct tcaactgttg aggtactttc tttgattctt actctaaaga cttttgaggc
    40741 gatattgtgc cgtatcgaga aaatcaagga ttcgaaaata tccgatcctt cgatcttata
    40801 ctcgagtaga ggattttttt gagcataact tcgcaagtat actgcttctc tgagagcttc
    40861 catcgattct aggtgattta gccatttttc atcaatttct tgcaaatacg aaaatctaat
    40921 gaaaaaattg aggctttcgg ctcccgctgc ttgtatcttt gaatcgagat caagctcaag
    40981 tttattccta atcgcaggaa taagctcgtc tgacttgtaa agagcagtca actcttttgc
    41041 tgtcatttct atgccaaagc tatcaaaaag ccataagcta aattcttgaa ttgctttgtt
    41101 tggattatcc ttgaattcct gggtgagaat agcattctga tcttctagca tttcgtaggc
    41161 tgcatcgcgt actctctcta taagattgtc atcagctagg attgagttcc tctgttcata
    41221 aatgaagtta cgttgcttat ttagaacatc gtcgtattca agcagatgtt ttcgtatttc
    41281 gaagtttctc tcttcaacct ttttttgtgc gctttcgatg gatttgctga gtagaggatg
    41341 atagattggt tctcctggtt tcatgccagc tctgcccatc agttgtttga aattgtctcc
    41401 tccaaatagg cgcatgagat catcgtctaa agaaataaaa aacaccgagc ggccaggatc
    41461 tccttgacgt ccgcttctgc ctcgcagctg attgtcaatg cgccttgcct catgacgctc
    41521 ggtacctaag acgcaaagtc ctccgaggct tttgacttct tcataatcct tagtccatgt
    41581 ctcgtattct cgagataaga tctctctata tttttcttca ggtgcttcgg taccagcttt
    41641 tcgccttgca cggaactccg gatttcctcc aagcttaata tctgttccac gtcccgccat
    41701 atttgttgca atagtaactg ctcccttaga acctgcttct gcaattatta gggcttcacg
    41761 tgcatggttc ttggcattca agacctcgtg ctttatgcca tttcttatga gcaaggaaga
    41821 tagcttttct gatttttcaa tggataccgt acctactaga ataggttgtc cctttgtgtg
    41881 catttctctt attgcctcga taatggcatc gtacttctca gcttcattta gatatacaag
    41941 gtcattctca tcttttcgaa caacaggcct atttgtaggg actaccatta catcaagatt
    42001 gtatatttta ttaaactctt cagcttctgt ttcggcagtg ccggtcattc cggctagttt
    42061 tttatataat ctaaagtaat tttggaatgt tattgtagca agtgtgcgat tgcgcctagc
    42121 aatcttgatt cgttccttag cctcgatagc ttcgtgaaga ccatcggagt atcgtctacc
    42181 atgaagaatt cggcctgtaa attcatctac tatttgaact tgattatctt gtacaacata
    42241 atcgacatct ttagagaaaa gatgctgggc acgaaccgct tgagtaaaat aatggacaaa
    42301 ttgaaaattt tcagggtcaa aaaggctttc ctttattaat cctctcttct gtaacaatag
    42361 ttcaatatgg tttaatcctt cgcttgtgaa catgactcgc ttggattttt catctatttt
    42421 ataatctcct tccaatagtt cgcctttggc ttcgtcggga tactcgccgg tctcagggtt
    42481 cttcttgact tcgagaaggg aggaagcaag tcgattgacc tcatttacct tgtaagtgtc
    42541 atcatctgca gggcctgaga taataagtgg ggttctagcc tcatcgatga ggatggagtc
    42601 gatttcatcg atgatgcagt aataatgacc tcgctgcact ttttgatcca aagaccaaac
    42661 catattatcc cgcagataat caaaaccgaa ttcgttgttg gttccatatg tgatatcgca
    42721 ggaataagcg acttttctag ctatattatc catggaggag agaatgcatc cgactgtaag
    42781 cccaagaaac ttatagattt gccccatcca ttgactatcg cgttctgcta gatagtcgtt
    42841 tacggttatg atgtggactc cttgtcctgc taatgcattt aagtaagccg cggttactga
    42901 agatagagtc tttccttctc ctgttttcat ctctacgatt ttgccttgat gcagcgcaat
    42961 tcctccaatt atctgcacat cgaatggacg ctcacctagt tttcttcggg ctgcttctcg
    43021 cacaagagca aaagcttccg gtaaaagatc atcgagagtc tctcctttgg caaggcgatc
    43081 cttgaatttt atcgtcaaag cagggaagtc gctatcatca agagacagag cccaagactc
    43141 tagttcgttg attttatgga gaagtggcaa aacagctttg atatcttttt cttcttttga
    43201 acccaagagg gcggaaagga ttgtatttct agccatgcat taagaatact tttgttctca
    43261 aaaagggtca aggaatggta taatttcgaa aatgaagaga tttgtaagta ttttatgctt
    43321 gctagtagtc gtagctgatt tccttataag ctgccactcc tccgtagaag aaggaaagat
    43381 acaagcgctt tttgaccttg gttatggcag atctgattca tcgatcgata tatctatcca
    43441 aaataagaat tcagttgata tttgccttag gaaaggaata tttcatctgc tgagtcgggg
    43501 agaggcaaaa atccttcgca tttcttctta tggccagact cttgcaatgt ggtatgaccc
    43561 taaaaaagga agtgcacctc ttgtcctcaa agatgtccag atgagtgact tttcttcaga
    43621 ggaagatcta ggccgttttg caattcaggt ttcattcaat gatcctggct atattgctac
    43681 agattcaagg caaatgctct atgtcgctga tgctcaagtt gtaaggaggt ttgattccga
    43741 tggtagggag ctgccttttc ttggacaaga aggcattgga ggaattagat tcccagtaat
    43801 aactgcactc gatgttctca gcaatgatga tattgcggtt tcctgcgcat ctgagtcgga
    43861 aaaagcagtc tatttctatg atcgaaatgg tacctttttg caagtgctaa aatgggatgg
    43921 gcagcatatg cccgttcctg tttcagttgc ggagaatcat ccaaatgcaa gtggctcgcg
    43981 cataattgcc tcgcttgagt catttctacc gtgcttcttg aatgggaaaa gagtagtaat
    44041 tctcaagatc aactattatt cggagaaata cgatccttca tcgggagtga caatttcgat
    44101 cgagcctatt ggtggttgga ttatggtgac agatgcatca agcggcgcat ttttgaattc
    44161 ttttcctttg cagagtagtc ttgaagattt gggtatcgat caacagatga tcgcagcaca
    44221 atcgaatgat attttctgta ttcgttggag agatgtaggg ttggccggag aggttttccg
    44281 ctttaatcaa cagggaagga taaatggaaa attggatttt caagtacctg atgatggtgc
    44341 ttctcttata gctatgatca ttggtgatga tggaaattta tatatgctta ctcaattatc
    44401 ttccaagctg aggatgtata catggaaaat actcaccttg agatgagata gcttattcaa
    44461 acccttgatc cctttgatat gcagagaaaa taatatgcaa aagattcaga acaattatga
    44521 tgacattcta tagcatttcc gctatagtca tttcgctatg aatttagaag catttgtcct
    44581 cggatgtggt ggcatgatgc ccctccctta tagacatcta actagcatgc ttatacgtag
    44641 agagggggag ttgtttcttt ttgatgctgg agagggaacc caggtttcga ttcgaaggtt
    44701 aaatctacgc tggaaaaaga tcactgctat ttttatctct catatgcatg ctgatcatgt
    44761 tactggcctg ccgggcattc taatgctctc tagccaggtg gatagggaag aaccgctata
    44821 tatttttggg cctccaaaga ttgcagaatt tgttgatcag aataggcatc tcttggatat
    44881 gtatatcaat tacgaaatca tcgtaagaga gatatatgaa gctggaacta tatggcaggg
    44941 tgatgattat tcgataagag cctttcctct aaagcatacc aaaatgtgct ttggttatgc
    45001 gttggaagaa tctaagcgtc ctggcgtttt ttatccagaa aaggcccttg cgctgggtgt
    45061 ccctcgtggt cctctttggt ctgagcttca gtccggcaag acagtccggc ttacagaagg
    45121 aagctctata aaaccttcag aagttatggg cgagcctcgt agcggaagga aattcagttt
    45181 cgttacagat acgctttatt tcccagaaat tgctcaagag gtatcaaatt cggatcttct
    45241 tatatgcgaa gggatgttcg aacacgcgct tattgagagc gctgttgaca agcggcatat
    45301 gactgcacat caagctgctc tgattgctcg tgatgctggc aatataaaaa agctgggctt
    45361 aattcactat agcccgcgat atgctgatcg agagctcaaa attttactcg acgaagcccg
    45421 agaaatattt cctgataccg tgcttacaaa ggacagaatg cgtttcccca ttgagtttgt
    45481 cgattaggcc ccatatcact gttttatgct atagcgctag ctggcttaaa tgccaggatt
    45541 cttgagccgc ttctttcctg tgcttcttca agtgctgcaa aagcctccct atataggcga
    45601 tcagggaaga tcacgcgatc atatcgggcg cttatcccaa aatagaaggg tggttcttct
    45661 gatagatctt tatagagact agcggtggtt gtgagcacat catctagatc cactgccact
    45721 ttgagtgctg aaccagcatc tgtgccgggc aaaacagcag cataacagcc ttttccaagc
    45781 tcgaagacga gcgattccga tgcaaaatag tcgcgaatag tgactcctaa agccaaggcg
    45841 cttgggtcta actcaccaga aagtatacag tgaataagga gcagagatgt ttcttgatcg
    45901 cgttgaagct caatatcaag gttacgctta agttcagcaa gtgaaagggt agagggaaaa
    45961 acctcgggca gtggtacttc ttcggtttcc tcatcggtct gaggtgcagg ggcaacgaat
    46021 tctccaggca ggaaatcatc agaagatgag agaggctttg gaatcgcttc ggattctgat
    46081 tgggcagctt gaatattttc tggagctctt acctctttct gggcaatttc ttcttctctt
    46141 tcttcttctt cttcttctgc tgctgcttct gcttttgctt cttcttctat attttctata
    46201 gtctcttgaa ctgcggggtt tttgatgttt tcttgggttg cagaaagagc ttcttcagaa
    46261 tatgaggcac tctctaatgg attctcttga tactcttgct tttcaatagt ttcagttaat
    46321 tcttctttaa caatttcttc ttcaggctct ttaggtttct cctttaatac aagttgcaat
    46381 accacaagaa gcacaatcca tgcggaaagt atgatagctg gaacaataag aatattcgaa
    46441 atatctcttt gagataaaat tgaataaaga gccataagct tcatgccgtc gggaaggggt
    46501 gtcatgtaga ttattgtcga catacctgga gcatgaaata cgctgccagg atttgtagct
    46561 ggcgaagcaa agtagggcga ctcatcgggc attttccaga gaacaagtcc gttgcgatca
    46621 agtacttgaa cagcaagcaa atgtgtgctt gcgttatata gggatttaag cctatctctt
    46681 acgaattgat cgccaagatc ttcttcgctt gctataagag atgctgcatt tcgcaggatg
    46741 gaatactcag cagatgcgct ctgttctccc agaaggcgta tctcaccgat cttccatgcg
    46801 acatatccga caatgagagc taaaagcaca aaagtgccaa tgaataagat tatacgcaat
    46861 gttcgtttca ttttttccat ccctgcagta tataatatca ttatcggcaa atacctgccc
    46921 agttggagag tattgtttat tgattcaaat gcacataatg catgggattg ccgctaggag
    46981 gataaaagcc atggctacaa gtcgcagaat taggaaaagg tacatgtctt cgggttggta
    47041 tcctgaagat tcagcagaaa tcagttctct tgtcgcagat tggactcaat ctcagaagaa
    47101 tctatcggca tatgctgttc tctcgcccca tgcaggatgg tatttttctg gggatttagc
    47161 tgctaagtca gtctgggcat tgagagattg cgatacagtg gcaattctcg ggggtcattt
    47221 gagtagtgga gatcccatcc tatatggcga tgaagatgat ttcgactgta ctgtcagaga
    47281 tgcagagaat gatatagaga tgcttaatgc cttaaaggtc gaattgaaga atgttgggat
    47341 ccaagaaatc gcttcagatc gagacataga taattctgtc gaaataatat tgcctcttgc
    47401 tgggcttcgt ttcccagaag ctcgtattct atggcttcgc gttccaccag attataaagc
    47461 caaagagctc ggagccgccc ttttcagggc ggcatcttca tgcaaaaagc aacttgttac
    47521 cattgcttcc agcgatttga cccactatgg ccctaattat ggttttatgc cacggggctt
    47581 gggagagcta gctgtccaat gggttagaaa tgaaaacgac agaggtttta taaatgcagc
    47641 gcttgagatg gatgccgaca aggtattaaa acacgctagg aataatttct ctgcctgttc
    47701 ctcaggagct gttgctgcag ctatatcttt tgctctcaat gcaggggcaa aacatagtct
    47761 tctaattgag cacaagctta gcttcgacat ccatcctgat cgttcatttg tcggatatgc
    47821 atctatcgct tttgttccat agagtcttat tcagcatatt ggcgaaaaag catgagggct
    47881 tcttttttaa aagaatcacc tcgttcaaac tcatgcataa acataccaaa gcttgcacag
    47941 cctggagaaa gaattacaat atcaccagga tcagcttgct tgtctgcagt ttgaactgct
    48001 tcatgcatcg aaaagtaagg tcccttgtaa gccactttgt ttgcatcgag tattggaatg
    48061 agacgatctg ttccacttcc cgcgagaaga attagcgctt ttaggctcct tgctttttga
    48121 gcaaaggccg aaaagtcact catcttatcg cttccgccag ctattagaac tatagggcta
    48181 gaaaagctct caatggctgc aattgctgca tcagggatag tagcagtgct atcattgtac
    48241 catcttatgc ctcgtgtgga gcctacgaat tcgagcctat ggggcactcc gggaaagctt
    48301 gcaagtccag acatgatgtc ttctgaaggc agaccaaatg cacgggctgc aaccgcagcg
    48361 gccaagagat tctttttttg atggcggcca ggaaccaaaa cctgacttgg cacgagtagt
    48421 tcttctggcc catcgaaatt gaattttcca cggccttcga gaaggtctac agatgaagag
    48481 aaagctccaa aattatcggg attacttagc tttgttttgc tttttataag ccaaccaccc
    48541 aaagctcttt tcccaactga ctcctcgagg gggctttcac tgtaccaaag aacacgagct
    48601 cgggtttcct ctgcaaaaga atgtccccat ttctgatcag cattacatac tgtccagcaa
    48661 ttatcatctt gtgattcata tattacacgt ttgtcggaga cataagtctc catggaatca
    48721 taataattca aatgatcggg caaaatatca cttagcaccg ctacaagcgg tttcaataga
    48781 tttctacctc gcaagtctcc aagttgccag cttgaaagct caagaattac agggacttct
    48841 gtatctgtct catctaggaa atcaagagga gaaactgtga tatttcctcc cagaagggct
    48901 tttttattgt ttttcaagag gacatgccag agcgcggatg caatggtcga ttttcctttt
    48961 gatccagtta ccgcgataag aggcgatttg gaatagcgta aaaatattga gatatcggtc
    49021 tctattcttt ttgcaaaagc tagataaggc gaatcgcggc gaacagcagg gttttttatt
    49081 actatatctg catcgatgaa atcttttttt tcatggcgac ctagaacata gcgaatcggc
    49141 aaacctttaa gttcatatat ggctggtgat agagtttcag cgccacgtag gtctgtgact
    49201 gtgacatctg ctccagcttt tgcaaagaat ctggcgctcg caattccgcc accatgaaga
    49261 ccaagtccca ttatggttat atgtttgccg cgaaattcat ccattttgct gataattccc
    49321 cttcatctta atttatttta tagtgttgga agaatttgac ttttttaaaa tatagcataa
    49381 tcggaatatc ttcgatagtc tgttctatcc aagaggagat gtaattatat gcatagaatt
    49441 cgaatattat tgtatattgt gtcagattgt gctaaaccat gatttttctt gacagatttg
    49501 gaattcaatc ataggcttga atgaattatg aaccgcatag aacatcatcc tatactttct
    49561 gcaaaaaaga atctgactct cgtgaatttt tcattcgatg gagaggaaat atccggttat
    49621 gaaggagaac cagtggcttc tgccctaata tccgaaggcc ggcatgtgtt ttcctatcac
    49681 tttcgggatg gctttcctca aggtcttttc tgtgcaaatg gtcaatgttc acagtgcaca
    49741 atgttgatag acggcattcc aaagaagtca tgcgtaactc ctttggccaa agggatggat
    49801 gtccggacaa ttcatggact gcccagattg ccttctcagg atagcccgct tgttcaagca
    49861 gaacgcagat cgattcgcac tgatattctc atcataggcg ctggacctgc tggactcgct
    49921 gcagctgctg agctaggcag tatgggcttt caggttcttg tagcagatga caagccacaa
    49981 gctggaggca aattaatcct ccagactcat aaatttttcg gttcagaagc cgattgctat
    50041 gcaggaacac gcggcatcga tattgctaat atactgcaag aagaagccct gcaatatcct
    50101 tccgtaacaa tactaacgaa tgctcctgtg gtaggtatat acaaagaccg aaaggctggg
    50161 atctacctta attacgaatc ttatttgctt gtcgagttta cggctctagt tgttgctgcc
    50221 ggagcaagag agaaagcact ccattttcct ggctgggatc ttcctggagt aatagcagca
    50281 ggagctttcc agacacttgt aaatcgtgat cttgtcaagg catatatgaa aattctcatt
    50341 ataggttcgg gcaatgttgg cttgatcgcc gcatatcatg cccttcaggc agacatcgaa
    50401 gtcgctggta ttttcgaagc cgctggcaga gttaatgggt atcaggtaca tgccgacaag
    50461 ataaggcgtt tgggaatacc aatacatcta aatacgacaa taacgagggc agagggctgt
    50521 caaaaggtcc agagagcgta tgtagctgca gttgatgaga gatttcagcc tatacctgga
    50581 acaacaagag actacgaagt agattctgtt ctaattgcag taggccttgc accatgcaat
    50641 gaatttttag aacaagccag acgatatggt atcatggcag tggctgctgg agatgcgtca
    50701 gagatagcag aagcttcgag tgctatctat ggtggcaaaa ctgcggcggc aaatcttgcg
    50761 ctcatgctag gactagggaa aaaaggagct attgatccag ggtgggaaaa aacccgaaaa
    50821 gtgcttgctt cacggccggg agattgctat gaacgttctc cgatagttcc tggtaaagaa
    50881 tggcgtccag tattcttttg ttcagaggag attccatgta atccatgtgc aacggtatgc
    50941 cccctgaggt ccatagagct aaggtcaatg caaggaaaca ttatggatct tccttacttt
    51001 gcaggagatc gctgcacagg atgtggagcc tgcgtagcaa tctgcccagg tctcgctatt
    51061 actctagtgc gaagggtcgc agatgataag gcacaggtag tccttccttg ggagtttaat
    51121 gtcgattctg gactcgagga agacatggaa cttgtggatc aaaaaggcga ggtattggaa
    51181 agagtcccaa taatatctaa acgaaaagtg cagaaaagaa atacatggct tttgaccttc
    51241 gaggtagatg tggcgcatgc agcaaggatt gcaggtgtta gacatctcaa aacgcagcaa
    51301 gttcctcgac cgcaaaagga tacaatagaa ttcaaaaata tacctagcat catatctgtc
    51361 gaaaagagtt cgcttgaaga cctagatact gtggtatgtc gctgcgaacg aattaccttg
    51421 cgagaaatag tcgatttcat caagaaaaat gatgtcaaag acgcaaatca gctcaaatct
    51481 ctcagagtag gaatgggtgc atgtggagga aaaacctgct cacagctaat tgcaaaggca
    51541 ttcatcattg caggagtgaa tccagcggaa atcgagcctt ttactcagag gccacttttt
    51601 atggaagtgc ccatgggttc tcttgtgaat gaaggtttgc acaaattagg tggcaaagac
    51661 gcaggtacta gaggcgcgcc atgaaaaact atgatgtcgt aataatagga gctggatcgg
    51721 tgggcctacc tctatcatgg cagcttgcag agaaagggct caaggttgca ttgctagatg
    51781 aggaggcctc ctggggaaga ggtcagaatc gggctgctat aggcggaata agagctactc
    51841 actcggatcc tg
//
LOCUS       DAOSOB010000006        49037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_775_length_49037_cov_64.729799, whole genome shotgun sequence.
ACCESSION   DAOSOB010000006 DAOSOB010000000
VERSION     DAOSOB010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 49037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49037
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_775_length_49037_cov_64.729799"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PLS88_01260"
     CDS             <1..1110
                     /locus_tag="PLS88_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06020.1"
                     /translation="GGGSRDSDDRRGGGGLGGLWLTTNLFDSMIRLWFYSELFKGPEA
                     RYRSAQAKAQRRPLHKAVFSHVFGDGDPNAEWDTILKKAFVAFVQTHKGVISLPEFMA
                     ISGLKPEEAEDCITRYLVEFEGSPEVTENGALYFFFPSLLTKTATVSGVTASTFPTKK
                     LHRFSSNPEKMDRTFRWVNIFNLVFGSYYLWNAINLGTAIIVQTPEGTGLKGGLSFLY
                     SYTVYLFSMLGANQPVVLVGWLLGVAPLAFSILFFGIPIVRSLLIKRENEKIKHENMR
                     RVLYSAVIANPQYFDPHSIQLPANEVRPEKTNALLEETQHLAAWSRGEISPEGKWTFS
                     DIEFIEREVERVRASIDEKDYSPQKTVFDTDSPIS"
     gene            1159..2145
                     /locus_tag="PLS88_01265"
     CDS             1159..2145
                     /locus_tag="PLS88_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU06021.1"
                     /translation="MHGFLAAIISAISFSTLGIFAELLYYHGFSVLCALAWRFLVAAL
                     FLWFVVYIRKHIQSKSILAKSDIEKRKDNHGQQGLWKSVFLLGLFGFSPQAGLFFLTV
                     KILDPGITSLLLYLYPSFVFLIIFVWQHQKPKRIQVLALALSFMGCILTFWKAGSYPI
                     IGLALGILVAVAYAVYLVVAEHVLDGVDSLWATAIIMTAAALVYLCLALLEDTFLIPS
                     STEQFLIVLCIAIMASVLPIVTLFIAMQYLGARDTSIISTIEPVFTNILSILILGEKV
                     TIQRIIGGVSILAGVLILNRWGYASHKKDSATDLNGPSSPLSKSPGTDEGKS"
     gene            complement(2061..2897)
                     /locus_tag="PLS88_01270"
     CDS             complement(2061..2897)
                     /locus_tag="PLS88_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06022.1"
                     /translation="MYDKDSEERISQPIFNSLLSYLPEISRMRGWRMYARNGSRILSL
                     WSDYGRMLLGYSPRGFASIAKSSMDRGLFSATPGKWNSRIDKMLHGLLPEYQNIKILG
                     SQEKALTILQGLKGLASSAPSLASCRFLFDSYLRRDNKLPDTICLVLPIPSALSIGII
                     ASKLSTSLLMEQELIEGIKGMTSIYALSKMQSEEEKAIISRKLEPLWECFDLYKGTLF
                     SRSGPWLFPNYPREKHEEIFIYCLKNGLLISPDYDFPSSVPGDFDKGELGPLRSVAES
                     FL"
     gene            complement(2925..5114)
                     /locus_tag="PLS88_01275"
     CDS             complement(2925..5114)
                     /locus_tag="PLS88_01275"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOU06023.1"
                     /translation="MKLGQIPEYLEGLNPEQLEAVCYEGNRLLILAGAGTGKTRVITT
                     KIAHLVKQGKVLPESILAVTFTNKAANEMRERAMAIEPYCERAIIRTFHSFGAWFMRR
                     NAKAFDLRSEFTIYDEDDSAELVHSIFPAFNKRDCGDYAFLIAKAKDYCLAPDSPNLD
                     FISRNPEFRRIYSAYEDRLRSTGNVDFGDLILIPALLLEKDETIRNRTRQRFRVILVD
                     EYQDSNIAQFRLLQQLAGSDTMLCVVGDDDQSIYRFRGAEVKNILNFPKVFPDTKIIR
                     LERNYRSHQAILDLAHSIVIHNENRLGKNLLATRPGGNRPIIAFLDDQDQEVEFCAHT
                     ARQYVKGGGEWKDIAILYRTNAQSLGFERLFPQFDIPYRIVGAVRFYEREEIKDMLAF
                     LAILANPRDEISFKRIVNKPSRGIGATSLDKIISFSLHNGADLIEASRQTIASIPRKG
                     SEGLKEFLTLVEEAKTSFHKDMTVKENSGDLGSLISDFAQKSGLLEFYDNKDRISGSH
                     KKENIDELVNAASDHPLSEEGLLEFLDDVMLDQSRQRNENNEDSVTLITMHNTKGLEF
                     PIVIVTGLEQGLFPRNDEEGDDIEEQRRLFYVSVTRAKNQLYLTSCRWRRIHGKLFET
                     MPSQFISEIDPSLYKLWADSRNKMRAASPQYHSPSMVAPSQSNISAKKSEWEPGMSVY
                     HDEYGNGTVIKVTLSQSAGPLVIVKFETGKTAQFFPKYTKKLEKIKN"
     gene            complement(5111..>5813)
                     /locus_tag="PLS88_01280"
     CDS             complement(5111..>5813)
                     /locus_tag="PLS88_01280"
                     /inference="COORDINATES: protein motif:HMM:NF037386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOU06024.1"
                     /translation="CMLLDLMQALSNIEKDFRIRFLYIHPDHFPSEILQIIAHDSRFS
                     PYFDLPFQHASAPILKKMNRYGSAIKYLDLISRIRSELPMSMIRSTFLVGFPGETEDD
                     FEALLDFQKAANIDWLGVFSYSLEENTPAYSMKDRIPKSIARKRKAILEKEQMAITSN
                     RLRRFIGVTDSFIIEEAFCKDDLCFGRGWMQAPDVDGVTIINTSLKPGIVARAQIVSV
                     NGVDFNAELEYTATP"
     assembly_gap    5814..5913
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5914..6597)
                     /locus_tag="PLS88_01285"
     CDS             complement(<5914..6597)
                     /locus_tag="PLS88_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOU06025.1"
                     /translation="MPTFFMDQHGCAKNQVDGEEISSRLIDADFLAVSSPDEADAIIV
                     NTCGFIEDAKKESIGAIVEAKTRWPEKKVIAIGCLSQRYPDELFNNLPEADGVLGNGD
                     LSIIPEAVTKILSGERIMLVPVQSTAMPSPHYQRKVFFDYPGTAYIKITEGCSNYCSY
                     CAIPLIRGELRSRQIEDVVLEAQNLISKGMYELVLIGQDLGNYGKDLSGQCMLLDLMQ
                     ALSNIEKDFR"
     gene            complement(6662..8023)
                     /locus_tag="PLS88_01290"
     CDS             complement(6662..8023)
                     /locus_tag="PLS88_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU06026.1"
                     /translation="MGKIGDIFREARLGKGLTLEQVADETNISKRFLQGIESDDFSGF
                     PGEVYTLGFIRNYAEFLSLNSSAMVELYRSDESTRQSETDKSESIEPPSPVEDSKKGE
                     ALTQELPFSSEVDKSAKENVEPPPLQRKPIGKSTKKQKSENTKIETNANIHVDRTLEK
                     SGSANFQPGEKTRTANIQLGRILPLVFLAIVLIVAAVSIIPRLRISPSAARVPTEYRA
                     EGLPFEQRLYPQDKVYIPISGDVISITLDSIKDKVLFNTPFGTIAVGLNEKSVMNPSA
                     DRERLIIAVSDYDPRKSQNGAMVNFDVEEALSSTESSSDIIVPSTSEKTPLSQDLIQN
                     QAGRTSQAQTSSTILFRSTGGPHPFYVNVSFIAPVFFRYEADRKEWVERYYRKGESIT
                     VNASNTITFWTANAQAIKVNVFQSAGKSAELIMGGPGEIAVQRLSWSNSQGGWAMIAT
                     PVD"
     gene            complement(8051..8737)
                     /locus_tag="PLS88_01295"
     CDS             complement(8051..8737)
                     /locus_tag="PLS88_01295"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOU06027.1"
                     /translation="MKRKYFVIIASIIACFLGVGALSAQTLITADQFFSQLADKYALV
                     SDYQADVQITAGHQPMTGTLLFKSPSLLRIDFSQPSDQVIVFDGKTLIVYLPQYRAVL
                     RQDSTDQGISVGAATLASKEGLSLLRRNYTIGWEQSPNPEPLDPGSNELVYRLMLSRK
                     SVSEGYKNIRISVSADTMLIRRLEGWTVSNDKITFDFKNIQLNRGISDNRFVYNAPAS
                     ANVYSNFLFQ"
     gene            complement(8734..10395)
                     /locus_tag="PLS88_01300"
     CDS             complement(8734..10395)
                     /locus_tag="PLS88_01300"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOU06028.1"
                     /translation="MFETSPLHAGRYSYVPKIPEIFLDSIQSVKPIPTETIEPERDRE
                     ALKRIFPNSYGKPAIKFIKGEGALEDKAIAVGVILSGGPAPGGHNVIAGLFDALKKAN
                     PQSRLIGFKGGPSGLIKGQYIDLSSEIVDQYRNTGGFDIIGTGRTKIETPEQFEESLE
                     NAKKLSLDALVIIGGDDSNTNAALLSEYFFQRGAPITVVGVPKTIDGDLKNEWIETSF
                     GFDTATRVYSELIGNICKDASSGRKYWHFVKLMGRSASHITLECAFQTRPNVALISEE
                     IEEKQMSLSQVVEIIANTIAARAAKGENFGIVVVPEGLIEFIPEIKKLIAQINDIIAT
                     SEAEYESLSRGEAQRSYIISRLPSECASLFSSLPESIQTQLCWDRDPHGNVQVSRIET
                     EKLLAEMTSRRLQEMMAVGAYKAKFDYQTHFFGYEGRCAFPTNFDADYCYSLGYDACL
                     LILHGLTGYIAAIRNLSAAPKDWLPGGIPIVSLMNIEHRHGEEKPVIKKAMVELEGKP
                     FKAFAAMRDKWALSSDYRIPGSIQYFGPPSITDTPFITLQLEQRS"
     gene            10527..11534
                     /locus_tag="PLS88_01305"
     CDS             10527..11534
                     /locus_tag="PLS88_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU06029.1"
                     /translation="MVRRILRAFIFFLLFGLFLTVSNAEQEYHVIQAGETLYSIAKSY
                     SIPYEMLASINSITDPSKIRPGVVLFIPQVHIVAKGETYYGLSKQYGITIQELKSANN
                     LSDSYVLKVGDVLIIPEKEVNFVQTVQNLPSLDSVAMSQPAISVPAQQSPSPPATTAA
                     PSNSYSSASSTSQAILPPASPTASSSAVLPSTNQPAQTSAQTKADSSKASVSSQRIPD
                     TMVNWPVDGKAQYMSGKLEGVMFQTARGASVRAVASGTVVSAGPSRGYGEVVFVQSKS
                     GFVYVYAGNESIIVKTGENIEPGKTIAKVGIDANDGNPIAYFFVFRNGQPVDPSLAPR
                     D"
     gene            11636..12622
                     /locus_tag="PLS88_01310"
     CDS             11636..12622
                     /locus_tag="PLS88_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU06030.1"
                     /translation="MGDYSDCDKASIPKLPSRKGPIEKAEQESDPISLYLRQIARFPL
                     LNAQQEQEIGSRLEELSLFLKYCDREIANDPQNTALLEMKRDTLEKIRASKEVLITSN
                     LRLVVSIAKGYRMRGVSLLDLIDEGNIGLIEAVNRFDYRKGYRFSTYASWWIRQAIIK
                     CLMDQSRMIRLPIHMLNTIRRCYVSAKGLVQELGRNPNSIEIAEKSGIALSKVESALK
                     LALGTSSLDVCTEENQNVSLANNLKDENAPDPYSEAFLVTLRELLSYIMCSLSEREQT
                     VLELRYGLNGEAPKTLEETGLALGITRERVRQIQERALEKMRNRQELSDSIL"
     gene            complement(12765..15566)
                     /locus_tag="PLS88_01315"
     CDS             complement(12765..15566)
                     /locus_tag="PLS88_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOU06031.1"
                     /translation="MQKLSFKAGQLLSAIIAVLLLFGGCATSPAKNKEVTLNDLVIQG
                     DFEGIRKFYSNQEELNKPSNKEPYVGLYPLHIAVTQGNTQIAEILIVLGAKPDNQDLS
                     GKTALRYAVDRKQTAMVKMLIDRGADPFVPDVGGTSPAQLALHSDISLLDAMFNSRNI
                     NNTGPDGRTILHHAADALLPKEVDLLLSKGALVQIKDKADRTALDLALLYPEKLEAAQ
                     IAEKLILKGANPSFPEFSWFADTVRRPDYSAARFANGNTPLHEAISRYQYGFALFLLL
                     NNVDPNLKNLNGDAPLHLAVKSGYVEGARLLLSHNANPDILDANQSTPLHLTIPATQR
                     LEMVKLLLAFKANPAIMDNQGNTPLHKAVTQLYAPEIVETLIQAGAPVNAQNSKGDTP
                     LIICARTEHYEYAKPLIEAKAEIFKMNQAGENALKIALSKGYTAVDNIVLPSNVNQSD
                     DNSNSVLMTAVLLKANPDVLKLILSKGADPNAKNKAQDTALHIAVRQNYAQQGIVLLD
                     ANADVFQYNAMQENPLFLALTAKPAPLEWFFRPNVISATDANGDSVVHHAARKNLPDG
                     IAYLIKKGANIEALNNAGEIPLHTAAKYDSTDAIRYLISAGSSLNAANKNGDIPLQSA
                     VLNGAFKATQMLLAAGSPVNNQNYSGETALHQASKNNSAAIIQLLIAQGANTEIQDSR
                     GFTPLITAAANDRYDAASELIKAKALIDTRDYLGSTPLYHAVSGEYASLIRLFLVNDA
                     DIHALNAQELSPFRLSLSKNPQITSEFYTNKLINKPDSNGDCILHIIVKSGQGQEALT
                     EALKHGANLNARNSKGDTPLMLALKKGDLNTAVVLIQAGSSLFISNADNTLPLYVIFG
                     MDSGARMKLFSAAGTNSADFIGESLLHYAVRANNADIVSELLSLGADRNAQNRKGETP
                     KDIAQTKGYQEILAILARK"
     gene            15726..16703
                     /locus_tag="PLS88_01320"
     CDS             15726..16703
                     /locus_tag="PLS88_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOU06032.1"
                     /translation="MNIGSFMPIQSIWKTVIEQLREEFPDHTWLEGLTPDSPEIGSLD
                     LIIAGRIPADVIKAATSLKALFQPFTGVNHLPSQLMLERGIEVYNVHSNAFDVAEKAL
                     ALTLAFYGRVIEYHNDLKNEIWHGFWVNRGAEDNWDSLYGRKCSILGTGAIGQELAGL
                     LKAFHCTVYGWRRTKNGKLPDHFDAIVPSLQEAIDSAEIIFIALPATTETENLISKEM
                     LMSMKGKFLVNVGRGSIVNEEGLYEALKSGVLKGAAIDTWYTYPVTGVLGAPSRYPIH
                     KLSNVVLSPHVGGSTNQASARSIEDTVANIRSYLMTGRGIWKADLSKMY"
     gene            complement(16757..18571)
                     /locus_tag="PLS88_01325"
     CDS             complement(16757..18571)
                     /locus_tag="PLS88_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU06033.1"
                     /translation="MKLLFVQLPAQLPDWSSVPANVPLAAGYLASYAESKGLLSRREW
                     TILEPPIANYGSDASIISSIVAREPDIIAFTLYSWNIKRSLFLAEKISSQLPRARLIA
                     GGPEVVKSMPITSKSPFNSLIYGEGEEAFTDILQDIQEHRPISSFYSSDNLVDLNKLP
                     NPYLSGALYFDPNFQAHIETMRGCNSRCSYCYYGKNYKTVRYFPHEQILEVIRLASEA
                     GVPEIYIMDPNFQTGSDFAGKLRDIAFVNHAHTPIHTELRLEGLDEEIAGLLKEAGII
                     SVEAGLQSTNSKALEAVHRSFDRKAFERGAELLQRQKIIIKTGLILGLPYDGYEQIIE
                     TFDFLGMQGLGQEAELYPLSLLPGTEIRERADEYGMSAMDAPPYLVTSTHWISYDDMV
                     DAISAFEESFDVEWAMPPAPHFQLFKEGFVSFIDIRKPENIDWMRLNPEKLSNSITLL
                     IDADDPETLSRVVRAARDLRKDNPYTLYQIVLTSETRIPSEKLVERIRDAFLNSDHYY
                     ELLNSFSPDPQTSYQTRIFFATKNFALAYRALEEAQDMETMVILSGKGGYNSDRLSEL
                     LPYVIFDKLSLPFDRLYELISIYADFPHMLIEAPEGLF"
     gene            complement(18636..19424)
                     /gene="aat"
                     /locus_tag="PLS88_01330"
     CDS             complement(18636..19424)
                     /gene="aat"
                     /locus_tag="PLS88_01330"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOU06034.1"
                     /translation="MRWIVMPDRLDEEQEYRFPPAESATPNGVVCYGGNLSPGALISA
                     YRQGIFPWPSNPAFIQWCSPDPRFIILRGFLHVTESAQKALKKALKPNSGYALTLDNN
                     FQEVIKNCAAMPRAGQPGTWIFPNIVDAYTRLHEIGYAHSVEVWKNGNLVGGLYGVSI
                     GMAFFGESMFSLESNASKIGFLSLAKTLFNQGFEFIDCQVYTPYLELMGGIDVPRLIY
                     LSILDAALKKPTMKGSWSKMFPGFPERELIMSADNNHEVMVDRQ"
     gene            complement(19443..21725)
                     /gene="clpA"
                     /locus_tag="PLS88_01335"
     CDS             complement(19443..21725)
                     /gene="clpA"
                     /locus_tag="PLS88_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HOU06035.1"
                     /translation="MKVSSEVQAIFNAAYNEAKLRNHEYLMPEHILFASLSFEKVRAI
                     FESCDADLEQMRRSIEAYFEQKMPIVRNAEPIQSAGFQAVIERAVMQSQSAGKEEVQI
                     SDLVVSLYDEERTYAGYLMRKLGIKRLQLLEVLSHGINDDDISSDALSDEEAEDRAEE
                     GARDKRTLRPGTLERFATELTALAAAGKLEPVIGREAEIERTIQVLSRRLKNNPIHVG
                     DAGVGKTAITEGLAQRIAAGNVPPKLKGYTIWSLDMGALLAGTKFRGDFEERVKKVID
                     LLLKKEKAILFIDEIHTIIGAGAVTGSTLDASNLLKPALTSGKLRCIGSTTYDEYNKI
                     FEKDRALSRRFQKIDIVEPTIPETVEILKGLRPKYEEYHNVQYSDETLEIAVKLAAQF
                     ITERKLPDKAIDVIDEAGALARIRADKEKDETDTATVDISPHDIEVVIAKIARIPERT
                     VSSSEKDKLATLEDSLKQEVFGQDQAIEAVVKAVKRSRAGFRAPDKPIANFLFVGPTG
                     VGKTELARQLSKHLGITLHRFDMSEYQEKHTVSRLIGSPPGYVGYEEGGLLTDAIRKT
                     PNAVVLLDEIEKAHPDIYNILLQIMDYATLTDNQGRKADFRNVILIMTSNAGARDIGK
                     PLIGFGDQQVSSSALDEAVERAFSPEFRNRLDAVVHFNNLPMEIIERIVKKAVDEFAM
                     QLAEKNVTLKVEDEVIRYLAIKGYSREFGARNISRLVEDQIKTVFVDEVLFGKLEHGG
                     MAMARLENEKIVFDISPQKD"
     gene            complement(21722..22039)
                     /locus_tag="PLS88_01340"
     CDS             complement(21722..22039)
                     /locus_tag="PLS88_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOU06036.1"
                     /translation="MGTRVEQSIDTQELTREEIEAREPEEYRVYLINDDFTTIEFVIS
                     ILMKVFHKNLMEATKLTMEVHRKGRGLAGVYPYDIAITKVQQVHAMARQRGFPLRCAM
                     EKT"
     misc_binding    22143..22408
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            22480..24204
                     /gene="thrS"
                     /locus_tag="PLS88_01345"
     CDS             22480..24204
                     /gene="thrS"
                     /locus_tag="PLS88_01345"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOU06037.1"
                     /translation="MDIEKIRHSLAHVMAEAVQHLFPGVKFGIGPAIDDGFYYDFLFP
                     QPITNDDLSAIEKEMRRIIEKDQDFIRTEISKQEAQELFRDQPFKLELIEGLEEGKIS
                     IYKQGDFVDLCRGPHVESTKELKPSAFKLKTIAGAYWRGDESKPMLTRIYAYAFESKA
                     KLEDYLHMLEEAERRDHRKLGKELDLFSTHEEAGPGLIYWHPKGGRFRVELENWWRDE
                     HYKNGYEILFTPHIGKSWLWETSGHLGFYKANMYAPMKIDEDEYFIKPMNCPFHIMIY
                     KTSSHSYRDLPLRWAELGTVYRYERSGVLHGLMRVRGFTQDDAHIICTPDQIEDEILE
                     VLRFSLSMWKTLGFKEIKAYLATRPADSVGEPERWDQALVSLKKAIDKEGLLYEIDEG
                     GGAFYGPKIDLKVKDAIGREWQMTTIQFDFNEPERFDMVFIDKDGKQKRPYMVHRALL
                     GSLERFFGVFIEHYAGAFPVWLMPDQVAVIPVAPSFDAYAKTVAKFLQEKGIRAKAML
                     SEERMNAKIRDAQNQRIPYMAIVGQREQEGSLVSLRLRTGDQESGIPLEVFAARVLEK
                     IDMKSLDL"
     gene            24263..25249
                     /locus_tag="PLS88_01350"
     CDS             24263..25249
                     /locus_tag="PLS88_01350"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOU06038.1"
                     /translation="MADLRTYLMGLQLQNPIIIGASGLTSMPDGVEKAAHSGVGAIVL
                     KSLFEEQILAVLGQEMAGLDLNGYPDAEAFISRTAWEESTDEYLRLISESKRKAGGVP
                     IIASINCVGQGRWADFARRIEEAGADALELNIAYVPFSPQISGQEIEKKVLSTVKETR
                     SVTKLPIMVKLGNHYSSLPHLVHGLSKEGANAVVLFNRFFRFDFNLNTMRLSGAQPLS
                     SETEYHESLRWTSILYKRVGLEIVSSTGIHNANAAVKCLLAGASAVEVCSIIYKKGWK
                     IVEAILKDMNILMDSLGYSSLDGLQGKLAAINADRPEEYHRLQYIKALTDIY"
     gene            25448..26908
                     /locus_tag="PLS88_01355"
     CDS             25448..26908
                     /locus_tag="PLS88_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOU06039.1"
                     /translation="MKLNLSLDNLSEAFPEDLTPDQRARAQTIFLKTLSEKTHRFYGG
                     KIQIVPKAGLYGFNWFNVWYTPGVSKVSTTIRDDNDQSFALSNRGNLVAVVSDSTRVL
                     GDGDCTPPGGLGVMEGKAFLMKYLGGVDAIALCINSRGTDGKPNPDKIIDFVKMLEPS
                     VGAVNLEDISQPNCFKVLDELREACDIPVWHDDAQGTACVTLAGLLNALELAGKKLSE
                     AKIVLLGAGASNTTIARLILADGGRGENMVIFDSKGGLHRNRKDIETDKRYYRKWELC
                     LQTNPRCIETEEEALKGADVLIALSTPGPDTVKREWIRSMAPKSIVFACANPVPEIWP
                     YAAKEEGAYIVATGRGDFPNQVNNSICFPGLLKGALLVRSRKITDNMAIRCAHSIADF
                     ARSRGISADNIIATMEETEVFAREAADVAMAAIEEGVARLPDTWDNIYSKAKADILAA
                     RSLTQDLMKDGHIAAIPEEMLQEAFDFAVSEVRKKA"
     gene            complement(26962..28641)
                     /locus_tag="PLS88_01360"
     CDS             complement(26962..28641)
                     /locus_tag="PLS88_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOU06040.1"
                     /translation="MSTITLTIDGYKEISVPMGSNAEEIFIQSGMEQEWGTSTHPFPP
                     VAALVNNELAALSAPVNADCKIAPIYPDSPMGAEVYRHSLCFLLAMAAKAIVPKRRLI
                     VSMAIGNGYYHYFDDEEPVSPHLLNSLCLYMRDIVAKNLTIRIIMQSWNSVFEYFEQS
                     NQAETFSLMEYINDPFVLLNECQDYKDLHIAPLVPRTGLLSVWELMPYRRGMLLRFPH
                     TKKPYEMDPFIDIPVLYDIAEEYEQKAKVLNAGSIGALNKINQSGGIQDFILMAEALQ
                     NKKLAAIADMIAEKSDKTKVILIAGPSSSGKTTTAKKLSVQLRALGFKPIHIELDNYF
                     VDRSHTPLDKDGKPDFECLEALDIEFLNRQLLDLFEGKEIELPEFDFKSGIRKPSGTF
                     ISIRENEILMLEGIHGLNERLTPHIPADNKLKIYVSALTQLNVDDHNRVRTTDYRLLR
                     RMVRDYNFRGHDARETLSMWSSVQRGERLYIFPFQGDADIAFNSALDYELGVLKVFAE
                     PLLRTVKPHHAEYTDARRIQAFISRISPISPQYVPSDSILREFIGSSIFKY"
     gene            28706..29725
                     /locus_tag="PLS88_01365"
     CDS             28706..29725
                     /locus_tag="PLS88_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU06041.1"
                     /translation="MSKVTIKDIAQRAGVSKTTVSFALNYPDRISKKTYNKIMAIIDE
                     LGYVPNPFARSLTTKRIGAIGLLLPQKIGDIFANPHMTQVISGIGEECERREFSLAIL
                     PLIRGRIIEAARKSYVDGLITIGVGPGHEVVSLLKQNHIPFVTIDGEESASIINIGID
                     QKNAARELMQHVLKKGHKRLAILALEPDTKPSGAELASIVVQKRLEGFQIALKDAGLS
                     ITDSRISIQNCKGSIQSGYESACHILSLDPRPTAIVAMSDISAIGAMIATKELGLEIP
                     EDVAVAGFDDIQESMLVSPRLTTIHQPDRDKGAEAARMVMNMLDGSSGQHIKLPYQLI
                     VREST"
     gene            29767..31569
                     /gene="pepF"
                     /locus_tag="PLS88_01370"
     CDS             29767..31569
                     /gene="pepF"
                     /locus_tag="PLS88_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOU06042.1"
                     /translation="MTKIIPERSDVPAEHRWNLASLFSTDSEWEKELVVFNTMIHDAE
                     KGKISLGTDRQAFLKTLKDFEQYLILEERLGYYADLRVTENEGDSVARGMFERYVNLS
                     TQGQAAWSWLSPAIQMLDDNFIDSCLKDSSFEDYRVFLSKLRRFKPHVLSDAEERLLA
                     LQSEPAQTPKDAFSVLTNVDFDFGTIETPEGPKPLTQSTFAYFMRLPDRSVRRKAYLQ
                     FYASFEAHRHTLATLYAGSVKLDKYIAKVRKYPSARAMALFPDNVPEAVYDNLVSTVN
                     RNIPALHRYYALRKEVLGLDSLRHYDVYVPLVKEIKIERSYEQAVDIVIEALKPLGEE
                     YVNTLRGGLLGGWVDRYENKGKRSGAFSAGSFLGNPYILMNYKDDVLDDLFTIAHEGG
                     HSMHSWYSARNNPFMCYGYTIFEAEVASTFNEQLVFSYLYENSKDEREKAYLLANRID
                     NTLGTLFRQTMFAEFEERVHEIAESGEAITVDVLRKEYRGLLEKYFGPEMVFENVSDL
                     EGLRIPHFYNAFYVYKYATGLSAGIALSEGVLKGREKERDAYLNFLRSGGSRFPIESL
                     ELAGVDMSTPEPIEAACKRFAKDVEALSAILKAK"
     gene            complement(31642..32421)
                     /locus_tag="PLS88_01375"
     CDS             complement(31642..32421)
                     /locus_tag="PLS88_01375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOU06043.1"
                     /translation="MNILILDSSSETQFLSLKDRRTQHILEVLRKEVGGRMRAGSLDG
                     LIGWAILESISDAEIMLRFIAEESAPPLRPLRVLMGTVRPIQAARIVKELTILGVESV
                     VFVPTELGEKSYTQSHFYQQKEYLIYAREGAEQSGNPRLPEIGLAWTLDRALIKAERT
                     ESMDHADTKTPISARETDNSKCTRLLCHPDPSAQLLGDIILSGLPVVLAIGTERGWTN
                     HEIDIFLNVGYLLCSLGDRILKTETAASAAVSILLSRLGYM"
     gene            complement(32418..33632)
                     /locus_tag="PLS88_01380"
     CDS             complement(32418..33632)
                     /locus_tag="PLS88_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HOU06044.1"
                     /translation="MIIKPMIRNNICLNAHPEGCREEVRLQAARAKTFRKRLASSDIN
                     GSAPKPNNALIIGCSTGYGLASRLVAAFGYGAKTIGFSYEKEPSATKPATPGWYANKE
                     FDRLATENNLFAASFSQIDAFSHAAKQKAIEIALENNFKYDLVIYSLASSVRVDPDTG
                     VLYRSVIKPIGRQYAGRTIDVFTEKFSEATIQPATEEEIANTVKVMGGEDWKLWIDAL
                     QNEGLLAPEAITLAYSYIGPPFSWPIYRDGTIGQAKLHLEKTANLLEKDYGKENGLRA
                     FVSINKAVVTRASAVIPIIPLYISTLFKVMKERNIHEDCLDQMMRLFSERLYQKIGIA
                     IPTDDEGRIRIDDLEMGPSVQNEVSSRLARIDESNLHELADLAGFRSDFLRAHGFDVP
                     GVDYEREILSLE"
     gene            complement(33629..34048)
                     /gene="fabZ"
                     /locus_tag="PLS88_01385"
     CDS             complement(33629..34048)
                     /gene="fabZ"
                     /locus_tag="PLS88_01385"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOU06045.1"
                     /translation="MKNIESYLPHRPPFLFVDDVYIEGDTIIAQHAFHDDDWFFKGHF
                     PSFPIVPGVLLIESMAQAGGVGAKLMGIYPKSLFMFAKIKEARFRQAVRPGDTFMIEI
                     KNVRASSIYLHQKGIGKVGDAIAVEAEWISIASGVPE"
     gene            complement(34135..35415)
                     /gene="fabF"
                     /locus_tag="PLS88_01390"
     CDS             complement(34135..35415)
                     /gene="fabF"
                     /locus_tag="PLS88_01390"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOU06046.1"
                     /translation="MQNEVAVTGLGIISPIGNSIDEFWNNCINGLTGVGYITHFDASK
                     LESKIAAEVKNFDPSVWIDRKEARKMALFSQYAVAAATQAWMDAGLPDFAQNESLQNK
                     GGIDPDRIAVVLGNGIGGIEIFTESHAKMLQDGPDRIPPMTIPQMIANEAAANISMHF
                     GVHGPALTQVTACASGTDALGEALDLVRAGRVDIVIAGGTEAAITEFAVGSFCRLKAL
                     STSYNDRPELASRPFDRDRDGFVIGEGAGILILERLDHAKARNAKIRAIFAGYGSSCD
                     AYHLTAPQPEGIFGVKAIEHALRDADLQATDIEYYNAHGTGTDLNDPMESKMLKKAFG
                     SSVKSLKISSTKSMTGHCIAAAGAIEAIVCIKSLETGILPPTIHLDNPDIEQGCDLDY
                     IPNTAQARKIKTAMSASLGFGGHNGIIIFKAVGT"
     gene            complement(35518..36495)
                     /locus_tag="PLS88_01395"
     CDS             complement(35518..36495)
                     /locus_tag="PLS88_01395"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOU06047.1"
                     /translation="MSIVVRSISAYIPPLRITNEELAMRMDTTDEWIRSHTGIGARHI
                     APDGLQTSDMAVEAAKSALDKANIGPQELDYIIVATATPDYLGFPATACIVQDKIGAY
                     GVAAFDVTAGCTGFIYALNIASSMLEASHGRNALVIGAEGLSRFVNWEDRSTAVLFGD
                     GAGAAVLSRIDESARGCLSFILGAEGGGAKDLFLVQPERTKAFERQEPIVPFIYMDGK
                     KVYDFAVKSITVLIQRLLHKAAYKLEDFAWIVPHQANARIVQAAAKRLSIPLDKFYVN
                     IEEYANTSSASIPIALTEMSEKGLLKTNDLIMLVGFGAGLTYGAAVIRW"
     gene            complement(36705..38741)
                     /gene="ftsH"
                     /locus_tag="PLS88_01400"
     CDS             complement(36705..38741)
                     /gene="ftsH"
                     /locus_tag="PLS88_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOU06048.1"
                     /translation="MMPSKGKNNWKLDQQRKTDKNTNNRKKPGMPEGFQFRFSLGYVL
                     ITLLAVSFFNYFLFKSDTTLVPYSTFKDKVRTGEIRRVEIDANYYTGYTEPNPAEPSS
                     PLPPSAKPGVVYKTVPVSDPEFTSLLDEKGVVYSASPRGGSTILSLLLNWILPFAIMI
                     FFWRAIMSKFMGSNSNVLAFGQNKATVVAEGDIKTRFADVAGVDEAKAELVEVVDFLK
                     NPQKYTEIGGKIPKGVLLVGPPGTGKTLLARAVAGEANVPFFKMSGADFVEMFVGVGA
                     ARVRDLFKQAREKAPCIIFIDEIDALGKSRASGFIGGNDEREQTLNQLLVEMDGFDST
                     SGLIILAATNRPDVLDPALLRAGRFDRQVLVDKPDMKGREEILKIHTKNIKMDPSVDL
                     SKIARSTPGFVGADLANVVNEAALLAVRAGRKKVKEEDFQAAIEKVIAGLEKKNRMIN
                     PKEKQIVAVHETGHAITAAFTPGADPVRKISIVPRGYGALGYTLQMPLEDRYLITEEE
                     LLGKIDVLLGGRAAEELVFQSISTGASNDIANATEIAKRMITDYGMSDKFKNVALTRR
                     SSGLLSPQQASDPFATKEYSEETQRYIDETIASMINERYQHVVNMLTEKKYLLDHISS
                     VLLEKEVIEESEFAELLEGGTSIPKKLQEPMVAAEAAHLPGATEKASISSASGK"
     gene            39005..39271
                     /locus_tag="PLS88_01405"
     CDS             39005..39271
                     /locus_tag="PLS88_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOU06049.1"
                     /translation="MAVKPIGDRVLIKIKESEDKTAGGIIIPQTAQEKTQTGIVIAIG
                     TDADAIKVKVGDEVMYDKYAGTQVKIEGVEHLIVKMSDILAILE"
     gene            complement(39392..39844)
                     /locus_tag="PLS88_01410"
     CDS             complement(39392..39844)
                     /locus_tag="PLS88_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU06050.1"
                     /translation="MDLKEILKPELISLDLKGRNKEEIIRELVDLASNSGKVLDKEET
                     IQSVLEREYRMSTGMKHGIAIPHGKTTAVHELVACVGISKQEVDFDALDRKGCRIFIM
                     TLSPIDKTGPHLQFLAEVGMLFRSEEKRQALLEAKTKEEVVSILLGNA"
     gene            complement(39910..41139)
                     /locus_tag="PLS88_01415"
     CDS             complement(39910..41139)
                     /locus_tag="PLS88_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOU06051.1"
                     /translation="MKLWHKYALGIAMGAILYISLPPSMIHGTPTISFLAELALKIGG
                     YALILTLIAGIPISVFRLSEAHRFAKIFSQSLILSVASLIVAAGIGIAIVLIFKPVPL
                     PLVYQSGTVQTLDPLDLVRAIFPTSIFEVFLVSSKWFLPIGLFALFFGLALSHDPVMS
                     RPILPVLDVISRASYLINSFISEILGILLIPISVYLFLNLRNTGIPSEYRSVLLFVLA
                     LTVFSIFVLFPLIYRILGGKSNPYILLYAMSGPLLAAAASGSIFLSSATALRHVSESL
                     GIKRDTNAVVFPLVLVSGRVGSVIIVACSFIAMFLSYSRNNPTVGQIILLLALLPLSV
                     IVGASALRSDIIVMLSLACLLFGQGFQNGASLLVPIALPLSICATILDTAWLIFSLSL
                     IAELNGQRSVKKARNFI"
     gene            41272..41345
                     /locus_tag="PLS88_01420"
     tRNA            41272..41345
                     /locus_tag="PLS88_01420"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41306..41308,aa:Arg,seq:cct)
     gene            complement(41367..42587)
                     /locus_tag="PLS88_01425"
     CDS             complement(41367..42587)
                     /locus_tag="PLS88_01425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU06052.1"
                     /translation="MDEIEIQGKSKVQVCPVENKRDFERFLRFPWTIYGNDPLWVPPI
                     LPERKAILDPKRGEFFLRGGEAKLFLAIKEGRIVGTIAAAEDCSANTRDATKHCIFGF
                     FEYINDLDVARALIETVEEWGHSRGLTELYGPFNLDYENAYGVLVEGRDRPPVLLCGH
                     TPPYYLSTMEILGFLPARGDNIAIEIRLDKPIPDRAKLASLAASAREHRGYSIRNANF
                     FRFEDEVNCVLELLNKSLAHLPGFIAWEKPAVRALLEPFRSFADPRLVLFAMKEGKTV
                     GFFPAVPNLNEVLIKCDGFRTPYAALSGLIRMSLFSHPRTPEMRGIRRFLSRRYECVT
                     IKSVLVPPEHWGAGAAALLFDEMIRRLEGLGYSWIDLSLTSEDNPNTPALAERFGGKI
                     YKRYRVFRKAIASY"
     gene            complement(42600..44075)
                     /locus_tag="PLS88_01430"
     CDS             complement(42600..44075)
                     /locus_tag="PLS88_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HOU06053.1"
                     /translation="MFSRTQNAKIIEGKSGILTAYVPRLMILYMKDELGLSLKLAYGS
                     GDLGFSITTTMVGAYLSIFLTDVVGLAPTLVATAIFIGRSVDYINDPIIGHLVDRTRS
                     RWGRRRPYLLFGALPFGATFLLLWLRPPLNNPALLAAYYGGAYALFDTAATFVYMPYY
                     ALTPEIAPSYDQRTSLTSFRMSFSIIGSLIVFVLPWAFIGSFSPDNADRILVFAVGVA
                     IISVLPLFICFAAVHERPEFQKESVPNLKGSLKAAFGNRAFLFGAVIFLATWVCIDLL
                     QALIVYYIKHVIQRESYMDLIMALIFVTALISIPLWNFLSGLWDKRLAYIVGAAFLAI
                     CLGLLSFLGATTSMGVIAVLSVAAGIGVGAAHVIPWSILPDAVELDEYETGERHEGMF
                     YSLITLAQKAASSIALPLVLLMLGAAGYAPRAEKQSESAIRGIRLVMSIIPALLLGSG
                     AIFALKYPLKREDHKRLTAELDERRRNYADVQIDPAEAPHN"
     gene            44231..45463
                     /locus_tag="PLS88_01435"
     CDS             44231..45463
                     /locus_tag="PLS88_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU06054.1"
                     /translation="MKRFMLAFMALLTVTAAFGAGKLTLMQNKPEIDAQLKAYAATWG
                     QKNGVTVVIKSIGGTSGGMGPQLKADYAAGDMPDIFAFDGLEAYKEWEGIILDLSNEP
                     WVSKTSVPFKYNGKVYGFPVAIEGWGLAYNADLLSKAGIDPKTLNNFDAYKKAFEKLD
                     SMKKDLGINSVVSMAASIEMGWVTAHHNFNSLLSNGLPYGDLSVTNDLLAGKVDAQRL
                     QEYADWVELLFKYSDKAILLTGNYDAQVGAFATGKAVFLHQGNWVDPNLKDANTTFKM
                     AFAPHGSMKKVTDGIFVSAPSYYAINKESKNITLAKKFLNDLVMTPEGNNYMVKEAGM
                     IPAFSNVTLSPEGQLSKSVQQWSAAGKVYSWNQYYFSGDFRDKVLTPIYNQFAAGSIS
                     KAQFIDLMTKAFKDNAKK"
     gene            45591..46511
                     /locus_tag="PLS88_01440"
     CDS             45591..46511
                     /locus_tag="PLS88_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU06055.1"
                     /translation="MTKKTESSLVFWIFLLPVLFAFIMVMVIPFALGSYYAFTNWTSS
                     ARVDSSLKFVGFENFSNSLKDPSFLYSFGITFVYTILNMIVINIVAFVLALFVTGDLR
                     FKNLYRVGFFVPNLIGGIILGYIWQFIFNNALPSLGSIVPALDFLSDPANLMLSKPNT
                     AIAAMIIVGTWQYAGYIMMIYVAAIENIPQELIEASRIDGASTWVRLKSITIPLCAQA
                     FTVTMFLTLVNSFKQFDVNVSLTSGGPSTILMGQPIFGTELLALNIYNTAFISNKLSE
                     AQARAFIFFLILAIISIIQVNVNKKKEIEL"
     gene            46508..47374
                     /locus_tag="PLS88_01445"
     CDS             46508..47374
                     /locus_tag="PLS88_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU06056.1"
                     /translation="MKSARIRKPTSLLAEIVTAILFIVFLFPFFLVLINSAKTSFEVT
                     QYPLAWPKRWGTIVDNIVKIWTSESVRYPSSLLSSAIITAVSLILINLFSAMAGWALV
                     RTKTKVSSFIFFIFVASMVIPFQIVMFPLLSWFRTVTVATGIRLLRTYQGIILSYIGF
                     GAPLSIFMFHGFIKSIPLELEEAATIDGCHKHQIFFRIIFPILKPIQATVLVLNGIWI
                     WNDYLLPLLVLGKGNDVMTVPLAVSNFAGAFVKQWDLILTAILMAMIPVIVFFLFAQK
                     YIVKGMIAGAIK"
     gene            complement(47402..>49037)
                     /locus_tag="PLS88_01450"
     CDS             complement(47402..>49037)
                     /locus_tag="PLS88_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOU06057.1"
                     /translation="IAWYTLLENPAIEILDNGFRIRSKEEQLKLLEKERIRKEIAEAR
                     TAFVDAFSLAWKKRDPFFIEKNENFKPFVDELSRFACGLTSDSPIARDLGIRLTPQAV
                     HEALLATGFWKPSLNPWPERNGCILSIPSEPKNIQERPELALERLDLRHLPSFAIDNA
                     WSKDPDDAISIEGNRIWVHVSDPVSAIAPISAEDEDARTRASTLYLPEKTIPMLHHSL
                     IEAFGLGILPESRAISFGITMQKNGKIEEITIVPSIIRVTRLTYEEADVLLETDTVLK
                     NLNQIAEARKKLRQDQGAVDIEYPEISIRAIDDDVEFLPVPATRSAKLVQELMILAGE
                     AAARWAYSAGISFPYVSQEPPISSDLAGCGAGPSESLAENYARRKVMRTAFVSSTCAA
                     HAGLGLSFYSQVTSPLRRYQDMLAHYQIHAELARQKSSHRSDEETFIEAAIGLSREML
                     DERLYLYSQQVAKNRQAERDSRLHWTMVYLSKHRDWMGQATVLEVDRGNAQIYIPSLG
                     IEMPIRISRNIKLDTNITIALRRVSIPELSASFDVV"
BASE COUNT        13339 a        10594 c        10979 g        14025 t
ORIGIN      
        1 ggaggtggat ctcgtgatag cgatgaccgc cgaggcggtg gcggacttgg tggcttgtgg
       61 cttaccacta atcttttcga ttcgatgatt cgactctggt tctatagtga gctattcaaa
      121 ggaccagaag ccaggtatcg cagcgctcaa gctaaagctc aacggcgacc tctccataag
      181 gctgtatttt cgcatgtctt cggagatggt gatccaaatg cagaatggga tactatcctg
      241 aaaaaggcat ttgtagcttt cgttcagact cataaaggtg taatttcatt gccagaattt
      301 atggccatat ctgggctcaa accagaggag gccgaagatt gcataacaag gtatcttgta
      361 gaattcgaag gcagtcctga ggttacagaa aatggagcac tctacttttt ctttccatcc
      421 ttgctaacaa agactgctac agtatcaggt gtcacagcct ctacgtttcc gactaaaaaa
      481 cttcatcgct tctcttcgaa cccagaaaag atggatcgaa ccttccgctg ggtaaacata
      541 ttcaaccttg tatttggctc atattatcta tggaatgcta ttaacctggg caccgcaatt
      601 atcgttcaga ctccggaagg aacagggcta aaaggcggac tgagcttttt atattcttac
      661 acagtttatc tgttttctat gctaggggca aatcagcctg tggttcttgt aggatggctt
      721 ctgggtgtcg cgccattggc attttcgatt ctttttttcg gaataccgat cgttcggtct
      781 cttctaatca agcgcgaaaa cgagaaaatc aaacatgaaa atatgaggag agttctttat
      841 tcagccgtga tcgctaatcc ccaatacttt gatcctcatt ctatacaatt gccagctaat
      901 gaagttcgac ctgaaaaaac caatgccttg ctggaagaga cgcagcatct cgcagcttgg
      961 agcagaggag aaatcagtcc cgaaggcaag tggactttta gcgatattga attcatcgaa
     1021 cgggaagtcg agcgggttcg tgcatcgatc gatgaaaaag attactctcc acagaagacg
     1081 gtcttcgata ccgattctcc tatatcatag taattctggg gctttaacaa aatgataagg
     1141 gctcgtaaca gtaccgtaat gcatgggttc cttgcggcta taatctcagc catcagcttt
     1201 tctacacttg gcatttttgc tgaattgctt tattaccatg gattctctgt actatgtgcg
     1261 ctcgcatggc gttttctcgt tgcagccctt ttcctatggt ttgtagtgta tattcggaaa
     1321 cacattcagt cgaaaagtat attagctaag agcgatattg agaaaagaaa agataatcat
     1381 ggacagcaag gactttggaa atctgtcttt ttacttggtc tatttggttt ttcaccacaa
     1441 gcaggactct ttttccttac agtaaaaatc ttggaccctg gtattacaag cctgctgctg
     1501 tatctatatc cttctttcgt atttctcata atatttgttt ggcaacacca aaagcccaag
     1561 cgcattcaag tccttgctct tgcgctttct tttatgggtt gcatactaac tttttggaaa
     1621 gcgggatcat atccgattat aggtcttgca ttgggaattt tggttgcggt tgcctatgcc
     1681 gtttatcttg ttgttgcgga gcatgtcctc gatggagtgg attctttgtg ggctactgcg
     1741 atcattatga cggcagctgc gcttgtgtac ctctgtctcg ctcttctaga agatactttt
     1801 cttattcctt cttctaccga gcaatttctc atagtgcttt gcatcgcaat tatggcttct
     1861 gttttgccta ttgtgacgct ttttattgcg atgcaatatc ttggtgctag agatacctct
     1921 attatcagca caattgagcc agtattcact aatattcttt ctatattgat acttggggaa
     1981 aaggtgacta ttcagcgaat tataggtggt gtttctattc tcgcgggggt tttgattctc
     2041 aatagatggg gttatgcatc tcataaaaaa gattcagcaa ccgatctcaa cgggccgagt
     2101 tcacctttat caaagtcccc aggcacagac gaagggaaat cgtaatctgg agaaataagc
     2161 aaaccgtttt taaggcaata tataaaaatc tcttcatgtt tctctctggg atagtttgga
     2221 aaaagccatg gaccggagcg ggaaaagagg gttcctttat atagatcgaa gcattcccag
     2281 agaggctcta gctttctaga gattatagct ttttcttctt cagattgcat cttagaaaga
     2341 gcatagatcg aagtcatgcc ttttatgcct tcgataagtt cttgctccat caataggctt
     2401 gtcgatagct tgcttgcaat aattccaatc gaaagagcac taggaatggg tagcacaagg
     2461 caaatagtat ctggaagctt gttatccctc cttaaatatg aatcgaagag aaatctacaa
     2521 ctggctagac ttggtgctga agaggcgagc cctttcagcc cttgcaatat tgtaagtgct
     2581 ttttcttgtg aacctagaat cttgatgttt tgatattccg gcaaaagtcc atgcagcatt
     2641 ttatcgatgc gactattcca ttttcctggt gtggccgaaa aaaggcctct atccatggaa
     2701 ctttttgcga ttgaggcaaa gccacgcgga ctataaccta agagcattct tccatagtct
     2761 gaccaaagac tcagaattct cgaaccattg cgagcgtaca ttcgccatcc acgcatccgg
     2821 cttatttctg gtagatacga gagaagagaa ttaaaaatgg gttgcgaaat cctttcttca
     2881 ctatctttgt catacatact agagcgatcc tctcaatggc gatttcaatt cttgattttt
     2941 tctaattttt ttgtatattt cgggaaaaac tgcgcagttt ttccagtctc gaacttaact
     3001 atgacaagag gaccagcact ctgggatagt gttactttta tcacagttcc attcccgtat
     3061 tcatcatgat agacagacat acctggttcc cattctgatt tcttggctga tatattggat
     3121 tgagatggag caaccatgga tggcgagtga tactgtggag aagctgctct catcttattt
     3181 cttgaatcag cccatagctt atagagagat gggtcgatct ctgatatgaa ttggctcggc
     3241 atggtttcga agagctttcc atggattcgg cgccaacgac atgaagtcag gtaaagctgg
     3301 tttttcgccc ttgtgacaga aacatagaag aggcgtcttt gctcttctat gtcatcgccc
     3361 tcttcatcat ttcgcgggaa gagtccttgt tcaagccctg tgactatcac tataggaaat
     3421 tcgaggcctt ttgtgttgtg cattgtaatc aatgttactg aatcttcatt gttttcattt
     3481 ctctgtcgag attgatcgag catgacatca tccaagaact ccagaaggcc ttcctctgaa
     3541 agcggatggt ccgaggctgc attgacgagt tcgtctatgt tctctttttt atgggagccc
     3601 gatattctat ccttgttgtc atagaactcg agaaggccgc ttttctgagc aaaatccgaa
     3661 ataagcgatc ccagatcgcc tgaattttct ttaacagtca tgtctttatg aaatgaagtc
     3721 tttgcctctt cgacgagggt gagaaattct ttgagccctt cgctgccctt gcgaggaata
     3781 cttgcaattg tttgacgaga agcttctatc agatcagcgc cattatggag cgaaaaagag
     3841 ataatcttat ctagagatgt tgcaccaatt ccccttgaag gcttattaac aatgcgtttg
     3901 aaagaaatct cgtctcttgg atttgcaaga attgctaaaa atgccagcat atctttgatt
     3961 tcttcgcgtt cataaaaccg tacagcacca acgattcgat aaggtatatc aaattgcggg
     4021 aaaaggcgtt caaaacctag agactgggca tttgttcggt agagtatagc gatatctttc
     4081 cattcgcctc cacccttgac atattggcgc gctgtatgag cacagaactc cacctcttgg
     4141 tcctggtcat caagaaatgc aattattggc ctgtttcctc ctggtctagt agctaggagg
     4201 ttctttccga gcctgttttc gttgtgaatt actatgctgt gagcaagatc caatatagct
     4261 tgatgcgatc tataattgcg ctcaagtcta attatctttg tatctggaaa tactttaggg
     4321 aaatttaaga tatttttgac ttctgcgcct cggaaacgat atatagattg gtcatcatct
     4381 ccaactacac aaagcattgt gtcagaacca gcgagctgct gaagaagtct gaattgggct
     4441 atattcgaat cctgatattc atcaacaagg attactctaa agcgctggcg tgtacgattt
     4501 ctaattgtct catctttttc gaggagcaag gcgggtatca agatgaggtc tccaaaatcg
     4561 acattgcccg tagaacgtaa tctatcttca taagccgaat agatgcgcct gaattccgga
     4621 tttctactta taaaatctag attcggggaa tcaggcgcta ggcaataatc tttagccttt
     4681 gcaattagaa aagcataatc gccacaatcg cgcttattaa aagcagggaa tatcgaatga
     4741 acgagctcag cggagtcatc ttcgtcatag atggtaaact cgcttcgcag atcgaaggct
     4801 ttggcattgc gccgcataaa ccatgctcca aaagaatgga aagtccttat gatcgctctt
     4861 tcacaatatg gctcgatagc cattgcgcgc tcgcgcattt catttgcagc cttattcgta
     4921 aaggtcacgg caagaatgga ttcaggtaag acttttcctt gtttgactag gtgcgctatt
     4981 ttggttgtta tgactcgagt tttgccagtg cctgcgcctg caagaatgag aagacggttt
     5041 ccctcatagc aaactgcttc gagctgttct ggatttaatc cttccagata ttcaggaatc
     5101 tgtcctaatt tcatggtgtg gcagtatact ccaattctgc attgaaatcg acaccattaa
     5161 cagatacgat ctgtgccctt gccactatgc cgggttttag agacgtattt attatggtaa
     5221 caccatctac atcaggagcc tgcatccaac cgcgtccgaa acataagtca tctttacaaa
     5281 aagcctcttc tatgatgaag ctatccgtga ctcctataaa gcgccgtaat ctattggaag
     5341 ttatggccat ctgttccttt tccagaattg cttttctttt cctggcaatg gattttggga
     5401 tgcgatcttt catactatat gcaggcgtat tctcttcaag cgaatatgaa aacacaccga
     5461 gccaatcgat atttgcagct ttctggaaat cgagcagagc ctcaaaatca tcttcggttt
     5521 ctccgggaaa tccaacaaga aaagtggaac gaatcataga cataggcaac tcgcttctta
     5581 ttctcgatat cagatccaga tattttatag cagaaccata acggttcatt tttttgagga
     5641 tcggagccga agcatgctga aaaggtaggt cgaaataagg tgaaaagcga gaatcatggg
     5701 ctatgatctg caatatttca gaaggaaagt ggtcaggatg aatataaaga aagcgtattc
     5761 ggaaatcttt ttctatgttc gataaggctt gcatcaagtc taaaagcatg catnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcggaaa tctttttcta tgttcgataa
     5941 ggcttgcatc aagtctaaaa gcatgcattg accggataag tccttgccgt aatttccaag
     6001 gtcttggcca attaagacaa gctcatacat gcctttagat atgagattct gggcttccag
     6061 tacgacatct tctatttgtc tcgaacgaag ctcacctcta atcagcggaa ttgcacagta
     6121 actacagtaa tttgaacatc cttcggtgat cttaatataa gctgttcctg gataatcgaa
     6181 aaagaccttt cgttgatagt gtgggctagg cattgccgta ctctgtacag gcacgagcat
     6241 aattcgttct cctgatagaa tctttgtcac tgcttctggg attatggaaa gatccccatt
     6301 ccctagaacg ccatctgctt ctggtaggtt attaaataat tcatcaggat agcgctgcga
     6361 gaggcaacca atcgctataa cttttttctc cggccatctt gtctttgctt cgactattgc
     6421 tcctatcgat tcttttttgg catcttcgat aaaaccacat gtgtttacaa taatagcatc
     6481 agcttcatcg ggtgagctta cagctagaaa atccgcgtca atgaggcgcg agctgatttc
     6541 ttctccatcc acttggtttt ttgcgcagcc atgctgatcc atgaaaaatg taggcattta
     6601 agatttcctc aagatcttaa gcgatatcta tataataaaa ggattattgg cagcaagaac
     6661 tttagtccac tggagttgct atcattgccc aaccaccttg agaattggac caagataggc
     6721 gttgaaccgc gatttctcca ggccctccca ttataagctc cgcggactta cctgctgatt
     6781 gaaatacatt cacttttata gcttgagcat tggctgtcca aaaggttata gtattagaag
     6841 catttactgt tatagattct cccttgcggt aatatctttc aacccattct tttctgtcgg
     6901 cctcgtaacg gaaaaagact ggcgctataa aactcacatt tacataaaaa gggtgaggcc
     6961 caccagtgct cctaaaaagt attgttgaag aagtttgtgc ttggcttgtt ctccctgctt
     7021 ggttttgtat caaatcctga gaaagcgggg ttttttctga tgtggacggc acaatgatat
     7081 ccgatgagct ttcggtgcta gaaagtgctt cttctacatc gaaattaacc atggctccat
     7141 tctgagattt tctcgggtca taatcgctca ctgcaataat gaggcgttct cgatcggcag
     7201 aagggttcat gaccgatttc tcattcaatc ctactgctat tgttccaaat ggagtgttga
     7261 ataagacctt atcttttata gagtcgagag ttatagagat gacatctccg ctaataggaa
     7321 tatagacctt atcttgtgga taaaggcgtt gctcgaaagg caaaccttca gcacgatact
     7381 ccgttggaac tcgagcagca ctaggagata ttctgagtct cggtataatg ctaacggctg
     7441 caacgataag cactatagca agaaaaacaa gcggcaatat tctgcctaac tgaatattgg
     7501 cagtcctagt tttttctcct ggctgaaaat tagcagagcc agatttttcc aaagtccgat
     7561 ccacatgtat gttagcattc gtctctattt tagtgttttc tgatttttgt tttttagtgc
     7621 ttttccctat tggcttcctc tgcaaagggg gaggttctac attctcttta gccgatttat
     7681 cgacttcgga tgagaaagga agctcctgag tcaatgcttc tcctttttta ctatcttcaa
     7741 cgggcgaggg gggttcaata ctttcggatt tatcggtctc agattgtctt gtgctctcat
     7801 ccgatcgata aagctcgacc attgctgatg aattgagact aaggaactcg gcatagtttc
     7861 ttataaagcc aagagtatat acttcaccag ggaagccact gaaatcatca ctctcgattc
     7921 cctgcaaaaa gcgcttgctg atattggtct cgtcggctac ctgctctagc gtcaaccctt
     7981 tcccaaggcg agcttcgcgg aatatatcac cgatctttcc catcaccact ccttgcgtct
     8041 ttggcttctc ttactgaaac agaaaattac tataaacatt tgcactagca ggcgcattat
     8101 agacaaatct attgtcgctg atgcctcgat tcaattgtat attcttaaaa tcgaaggtaa
     8161 tcttatcatt cgagacggtc cagccctcca atctcctaat gagcatagta tcggcgctta
     8221 ccgaaattcg tatatttttg tatccctcag atacgctttt tctcgatagc attagccgat
     8281 aaaccagctc attcgaacct ggatcaagtg gctcaggatt agggctttgc tcccatccta
     8341 tagtataatt tcgccgaagc agcgaaaggc cttcttttga agcgagagtc gccgcaccta
     8401 cggaaatgcc ttgatcggtt gaatcctgac gaagcacagc cctgtattgc ggaagataga
     8461 caatgagcgt cttgccatcg aacacaataa cttgatctga gggctgagaa aaatcgatgc
     8521 gcaaaagaga cggcgatttg aagagtagag taccagtcat gggctgatgt ccagcggtta
     8581 tctgcacatc agcctgataa tcgctaacca atgcgtattt atcggctaat tgcgaaaaga
     8641 attggtcagc ggttatcaag gtctgggcac tcaaggctcc tacaccaagg aagcaagcga
     8701 taatggaagc aattatgaca aaatatttcc ttttcacgag cgttgctcca actgcagagt
     8761 tataaatgga gtatccgtaa tggaaggagg gccaaaatac tggatacttc caggaatccg
     8821 atagtctgag gagagagccc acttatctcg cattgccgca aatgccttaa atggctttcc
     8881 ttccagctcc accattgctt ttttaattac gggcttttct tctccatgcc tatgctcgat
     8941 attcatcaat gaaacaatag ggattcctcc aggtagccaa tccttcggag cagctgaaag
     9001 atttcttatt gctgcaatat agcccgtcaa tccatgcaga atgaggagac atgcatcata
     9061 tcctagcgaa tagcaataat cggcatcgaa attggtgggg aaagcgcatc ggccttcgta
     9121 gccgaaaaaa tgcgtctggt aatcgaattt tgccttgtat gctccaacag ccatcatttc
     9181 ttgaagtctg cgagaagtca tttcggctag gagtttttca gtttcgattc tggagacctg
     9241 tacatttcca tgaggatctc tgtcccagca tagctgcgtc tgaatcgatt ctggaaggct
     9301 agaaaacagc gaagcacact ctgaaggcaa acgcgatata atgtaggatc tctgtgcttc
     9361 tcctcgagac aaggattcat attccgcttc cgatgttgcg ataatgtcgt tgatttgagc
     9421 tatcagtttc tttatttctg gaatgaattc tataagtcct tctggcacta caacaatgcc
     9481 aaaattttca ccttttgctg cacgtgcagc tattgtatta gctataatct caactacttg
     9541 agacaggctc atctgctttt cttcaatttc ttcggagatg agagccacat ttggtcttgt
     9601 ttgaaatgca cattctaggg tgatatgcga ggcggatcgt cccatcagtt tgacaaaatg
     9661 ccaatatttg cggcctgaag aagcatcttt gcaaatgttg ccgatcaatt ccgagtatac
     9721 tctagttgcg gtgtcgaaac cgaaagaagt ttcgatccat tcattcttga ggtctccatc
     9781 gatagtcttg gggacaccga ctacggtgat aggcgctccc ctttgaaaaa aatactctga
     9841 aagaagtgcg gcattcgtat tggaatcgtc tcctccgatt attacaagtg cgtctaagct
     9901 cagctttttt gcgttctcta aggattcctc gaactgctca ggggtttcga tcttggttct
     9961 tccagtaccg ataatatcga atccgcctgt attcctatac tggtcaacaa tctcagagct
    10021 caggtcgata tattggcctt tgataaggcc tgatggcccg cccttaaagc caatgagtct
    10081 ggattgagga tttgcttttt tcaacgcgtc gaaaagaccg gctattacat tatgcccacc
    10141 tggagcgggt ccacctgaaa ggatgacgcc gacagcaata gctttatcct ctaaggcccc
    10201 ttcgcctttt atgaatttta tggctggctt gccatagcta ttaggaaaaa ttcttttaag
    10261 tgcttcccta tctctctcag gttctatagt ctcagtaggg ataggtttta cgctttgaat
    10321 agaatctaga aatatctcag gtatttttgg aacataagaa taccttccag catgcagggg
    10381 tgatgtttcg aacatctgaa tatacctctc ggaaaaaatg tcgatgacat tctaacaatt
    10441 tggcttttaa ttgaaaaggg cataaatagc aaattctttt cttcaatggc gacaaaagcg
    10501 tgccgatata taaactggaa ctttatatgg ttagaagaat tctcagagcc tttatattct
    10561 ttttgctttt tggattattt ctcacagtaa gcaatgcgga gcaagaatat catgtgattc
    10621 aagcaggcga aacattatac tcgatcgcta aaagctattc gataccatat gaaatgcttg
    10681 ctagtatcaa tagcataaca gatccgtcca aaattcgtcc gggggtggtt ctcttcatcc
    10741 cgcaagttca tattgtcgca aaaggtgaga cttattatgg tctttcgaaa caatatggca
    10801 ttacaattca ggaattgaaa tcggctaata atctttctga cagctatgtg ctcaaggtcg
    10861 gagatgtctt aataattcca gaaaaagaag taaattttgt acaaacagtc caaaacctac
    10921 cttctttaga ttccgttgct atgagccaac ccgctatttc agttccagcc cagcaaagtc
    10981 cgtctccgcc tgcaactacg gctgccccat caaattctta ttcgtcagca tcttctactt
    11041 ctcaggccat tctacctcca gcttctccta cggcatcatc gtcagcagtt ttaccatcta
    11101 caaatcaacc agctcaaaca tctgcccaaa ccaaggccga tagttctaag gctagcgtct
    11161 ccagtcaacg aataccagat acaatggtga actggcctgt agatggaaaa gcccagtata
    11221 tgagtggcaa actcgaaggt gtaatgttcc agactgcgcg tggtgcatca gtaagagccg
    11281 tagcttcagg gacggttgtc tctgcagggc catctcgtgg ctatggcgaa gtagtttttg
    11341 tgcaatcgaa atctggtttt gtctatgtat atgcgggcaa tgagtctatt attgtaaaaa
    11401 ccggagaaaa tatagaacca ggaaagacca ttgcaaaggt aggcatagat gcaaatgatg
    11461 gcaatccgat agcatatttc tttgtttttc gtaatggcca acccgttgac ccttcgcttg
    11521 ctccacgaga ctgacgaaaa agtattactg actttcctct tgtgtgctaa aaaacaataa
    11581 agtaaaattc tattggttat aattgtcgaa tcagcgaatt cgacaaggaa aaaatatggg
    11641 tgactattca gattgcgaca aagcgagcat tcccaagtta ccctcaagga aaggccccat
    11701 agaaaaagca gagcaggaat ccgatcctat tagtctatat cttcgacaaa ttgcaaggtt
    11761 tcctcttctc aatgcacaac aagagcaaga aatcggttca aggctcgagg aacttagtct
    11821 tttcttaaag tactgtgaca gagaaatagc caatgatcct caaaacactg cgcttctaga
    11881 gatgaagagg gacactctcg aaaagataag ggcatcaaaa gaagtgctga taacttctaa
    11941 tctgcgctta gtggtttcca tagctaaggg atatcgaatg agaggcgtaa gtctcttgga
    12001 tctcatcgat gaaggaaata ttggccttat tgaagctgta aaccgtttcg actatagaaa
    12061 gggatatcgt ttctcaacct acgcaagttg gtggataagg caggccataa tcaagtgcct
    12121 tatggatcaa agcaggatga tacgtcttcc gattcacatg ttgaacacga ttcgcagatg
    12181 ctatgtatcg gctaaagggc ttgttcagga acttggccgc aatcctaact ccatcgaaat
    12241 agccgaaaaa tctggtatag ctctgagcaa agttgagagc gctttaaagt tagcgcttgg
    12301 aacatccagc cttgatgtgt gcacagagga gaatcaaaat gtaagcttgg caaacaacct
    12361 taaagatgaa aatgcacctg atccttatag cgaagcattc ctagttacct taagagaatt
    12421 gttaagttat atcatgtgtt ctctttccga gcgggaacaa actgtactag agctcaggta
    12481 cggtctgaat ggtgaagcgc caaagactct tgaggagacc ggtctggctc tgggtataac
    12541 tcgagagaga gtgcggcaga ttcaagaaag ggctcttgaa aagatgagga atagacaaga
    12601 gctttcagac tcgatcttat aaaggcagct ttgaagcagt tctgtaaaac cttgataccc
    12661 ttaatagaga ggcaaaagac ctgcaagcct atagagttct agcatgcaaa tctgcatgta
    12721 gtactaaatc aggcatcctc tagatagcta ccttcattat catctcattt acgtgcgagt
    12781 attgcaagaa tttcctgata tccttttgtt tgtgcaatat cttttggagt ctctcctttg
    12841 cggttttggg catttctatc tgccccaaga gatagtagct ctgacacaat atctgcatta
    12901 tttgctctta ctgcatagtg aagcagcgat tcccctataa aatccgcgct gttcgtgcca
    12961 gctgccgaga aaagtttcat tctggctccc gaatccattc caaagattac ataaagaggc
    13021 agagtattgt ccgcattcga tataaagaga gatgatcctg cttgtatcag caccaccgct
    13081 gtattaagat cacccttctt tagagcgagc atgagagggg tatctccttt tgagtttctc
    13141 gcattgagat tggctccatg tttaagggct tctgtgagag cttcctgtcc ttgcccactc
    13201 ttcactatta tgtgcagaat acaatctcca ttagaatcgg gcttgttgat aagcttatta
    13261 gtatagaatt cagaagttat ctgaggattc ttcgaaagcg ataatctaaa aggagaaagc
    13321 tcttgggcgt tcagagcatg gatatcagca tcgttaacca agaacaacct tatcagtgag
    13381 gcatattcgc cgcttacagc gtggtatagg ggcgtggatc caaggtagtc acgcgtgtcg
    13441 atgagagcct tagctttgat taattcactt gcagcgtcat aacgatcatt ggctgctgct
    13501 gttatgagag gagtaaagcc gcggctgtcc tgaatttcgg tatttgcgcc ttgcgctata
    13561 agtagctgga taattgcggc gctgttattc ttgctagctt gatgcaaagc tgtctcgcca
    13621 gaataattct gattattcac aggagagcct gctgcaagga gcatctgagt cgctttgaaa
    13681 gctccattca ataccgcact ttggagagga atgtcgccat ttttgtttgc agcattcaaa
    13741 gaagatcctg cgcttatcag ataacgaatt gcatcagtag aatcgtattt tgcagccgta
    13801 tgaagtggaa tttctcctgc attattgagt gcttcgatat ttgcgccctt tttgattagg
    13861 taagcaattc catcaggaag attcttgcga gcggcatggt gaactacaga atctccattt
    13921 gcatccgtag cactgatcac gtttggtctg aaaaaccatt ctagaggtgc aggttttgca
    13981 gtgagcgcta ggaagagggg attctcttgc attgcattat attgaaatac atctgcatta
    14041 gcatccagga gcacgatacc ctgctgggca tagttttgcc ttacggcgat atgtaaggca
    14101 gtatcctgcg ctttgttctt agcattcgga tcagctccct tagaaaggat tagcttaagg
    14161 acatctgggt tggcttttag caatacagca gtcatgagaa cgctgttcga attatcatcg
    14221 ctttggttta cattagatgg cagcacaata ttgtcgacag cagtatagcc tttactcaat
    14281 gcaatcttga gagcattctc acctgcctgg ttcattttga aaatttctgc tttcgcttca
    14341 atgagaggct ttgcatattc atagtgctca gtccgtgcac aaataatgag aggcgtgtct
    14401 cctttggaat tctgtgcatt tacaggtgct cctgcttgaa taagagtttc gactatttcg
    14461 ggagcatata actgcgtgac tgctttgtgg agtggcgtat ttccttgatt atccataata
    14521 gcggggttag ctttgaacgc tagaagaagc ttgaccattt caagacgctg agtagctgga
    14581 atggtgagat gtagcggtgt gctttgatta gcatccagaa tgtccggatt tgcgttgtgg
    14641 gagagtagca gtctagcacc ctcgacatag cctgacttta ccgctagatg aaggggcgca
    14701 tcgccattca gatttttaag gttgggatcg acattattga gcaatagaaa aagtgcaaaa
    14761 ccgtattgat atctggatat cgcctcatgc aaaggcgtgt tgccgttggc aaagcgggca
    14821 gcactatagt cgggcctcct cacggtatct gcaaaccagg aaaactctgg gaaagaagga
    14881 tttgctccct tgagaatcag cttctcagct atctgcgctg cttctagttt ctctggataa
    14941 aggagagcga gatcaagagc agttctgtct gccttatctt ttatctggac tagggcacct
    15001 ttcgatagaa ggaggtcgac ttcttttggc aatagtgcat cggcagcatg gtggagaatt
    15061 gttcttccgt cgggaccagt attattgata ttccgagagt tgaacattgc atcaagaagg
    15121 cttatatctg agtgtagcgc taactgggct ggtgaagttc cacctacatc ggggacaaag
    15181 ggatcggctc ctctatcgat aagcatcttt accattgccg tctgtttcct atccactgca
    15241 tatcgtaaag ctgtttttcc agagagatcc tgattatcag gctttgcgcc aagcacaatt
    15301 aggatttctg ctatttgtgt attgccttgt gttactgcga tatgaagagg ataaagcccg
    15361 acatagggtt ctttatttga aggtttgttc aattcctctt ggtttgaata aaacttacga
    15421 ataccttcaa aatcaccttg gattacaaga tcgtttagag taacctcttt attctttgca
    15481 ggggaggtcg cacagcctcc aaataaaagt agcacggcaa taatagcaga gagcaattgt
    15541 cctgccttaa aagatagttt ttgcattctc tatatctccc ttcaagaata tcggcatcaa
    15601 taataaaaat cccgagtctc aacaatctat atcgcaaaaa tccaacaatt tcacaaggtt
    15661 gcttgcattt tagcattaag tatgcttcca tatatatgat atattttatt ttataagagg
    15721 ttatcatgaa tattggatca ttcatgccaa ttcagtctat ttggaaaacg gtaatagaac
    15781 aacttcggga agaattccca gatcatacat ggctagaagg gcttaccccc gattctcctg
    15841 aaatcggttc acttgatcta atcattgctg gacgcattcc agcagatgtt atcaaggcgg
    15901 cgacaagcct taaagctttg tttcagccgt ttacaggggt aaaccatctg ccttctcaat
    15961 tgatgcttga gcgcggaatt gaggtctaca atgttcactc caatgctttc gatgtcgcgg
    16021 agaaagccct tgctctaact cttgcttttt atggaagagt cattgagtac cacaatgacc
    16081 ttaaaaatga gatatggcat ggcttttggg tcaatcgagg agcagaggac aactgggaca
    16141 gcctatatgg tcggaaatgc tcgattctag ggacaggagc cataggtcag gagttggctg
    16201 gtcttctcaa agcatttcat tgcacagtat atgggtggcg taggacaaaa aacggcaaac
    16261 tgccagatca tttcgatgcc atagtgccct ctttgcagga agccatcgac tcagccgaga
    16321 tcattttcat agcgcttcca gctactacgg agactgaaaa tctcatatca aaagagatgc
    16381 tcatgagcat gaagggtaaa tttctggtca atgttggcag aggttctatt gtgaatgaag
    16441 aaggcttgta cgaggctctc aagagtggag tgctaaaagg tgctgcgatc gatacatggt
    16501 acacataccc tgttacaggt gtactaggtg caccctcacg ctatcctata cataagcttt
    16561 cgaatgttgt gctttctcct catgtaggag ggtcgactaa tcaggcctcg gctagatcta
    16621 tcgaagacac ggtagccaac attcggtctt acctcatgac aggtcgcggc atttggaagg
    16681 cagacctttc taagatgtat taaggccata tggaataaga ctttaaagtc aattggtttg
    16741 tgccgtctta aaagagttaa aagagtcctt ctggtgcctc gattaacata tgcggaaaat
    16801 cagcgtaaat ggagatgagt tcgtaaagtc ggtcgaaagg aagcgataat ttatcgaaaa
    16861 ttacataagg caggagctcg gaaagacgat ccgaattgta gccacccttc ccgcttagta
    16921 tcaccattgt ctccatgtcc tgcgcttctt cgagggcccg atatgctagg gcaaaattct
    16981 ttgttgcaaa gaatatcctg gtttgataac ttgtctgcgg atcgggggaa aatgagttca
    17041 aaagctcata atagtgatca gagttaagga atgcatctct aattctctct acaagtttct
    17101 cagaaggtat tcttgtctcg cttgtaagca caatctgata gagtgtatag ggattatctt
    17161 tgcgtaagtc cctagccgct cttactactc gggacaatgt ttcgggatcg tctgcatcga
    17221 ttagaagggt gatggagttt gatagttttt ctggatttag acgcatccaa tcgatgttct
    17281 cgggctttcg gatatcgata aaggagacga acccttcttt aaagagttga aaatgaggtg
    17341 caggtggcat agcccattct acatcgaagc tttcttcgaa ggcagaaatc gcatcgacca
    17401 tatcatcata ggatatccag tgcgtgctgg ttacgagata tggaggagcg tccattgcac
    17461 tcataccata ctcgtcagcg cgttctctta tctctgttcc gggaagcagc gatagaggat
    17521 acaactctgc ctcctgaccg agtccctgca tgccaagaaa atcgaaagtc tcaatgattt
    17581 gttcataacc atcatatggc aagccaagta tgagacctgt ttttattata attttctgtc
    17641 tctggagaag ttcggcgcca cgctcgaagg cttttctatc gaagcttcta tgaaccgcct
    17701 ccaaggcttt ggaattcgtg ctttgaagcc ctgcttcgac cgagataatg cctgcttctt
    17761 tcagaagacc tgctatctct tcgtcaagac cctcaagcct gagctcggta tgaataggcg
    17821 tatgagcatg atttacaaat gcgatatcgc gcagctttcc tgcaaaatca gaccctgttt
    17881 gaaagttcgg gtccattatg tatatctcag gaactcctgc ctcagaagca agtcgaatta
    17941 cttccagaat ctgttcatgg ggaaaatagc ggacagtctt atagttcttt ccataatagc
    18001 agtatgagca tcttgaattg cagcctcgca ttgtctcgat atgagcctga aaattcggat
    18061 cgaaatagag tgcgccactc agatatggat ttggtaattt gttgagatct acgagattat
    18121 ctgaactata aaaagacgaa attggcctgt gctcttgaat atcctgcaat atatctgtaa
    18181 atgcttcttc tccttcgccg tatataaggc tattaaaagg cgatttcgat gtaattggca
    18241 tggatttgac gacttccggt ccgcccgcta ttaagcgggc acgaggtagc tgtgaggaaa
    18301 ttttttcagc caggaaaaga ctgcgcttaa tattccatga atagagagtg aatgcaataa
    18361 tatcgggctc acgcgcaaca atagatgaaa ttatagaagc atctgatcca tagttcgcta
    18421 tggggggctc aagtattgtc cattctcgac gagagagcag tccttttgat tccgcatagg
    18481 aggctagata tcccgcagct aatggtacat ttgcagggac cgaactccag tcgggcagct
    18541 gtgcaggtaa ttgtacaaaa agtaatttca ttagtattcc gttcttcttt ttttgatgat
    18601 cctatcatgg atgatccaat atcttagatc tgctatcatt ggcgatctac cattacttca
    18661 tggttattat ccgcagacat aattagctct ctttcaggaa aaccggggaa cattttcgac
    18721 cagcttccct tcatggttgg ttttttaagt gcagcatcga gaatagagag ataaatcaaa
    18781 cgtggcacat cgatgcctcc catcagttct aggtagggag tgtatacctg gcaatctata
    18841 aattcaaagc cctgattgaa taaggtcttc gccaaagaga ggaatccaat ctttgaagca
    18901 ttgctctcca ggctaaacat cgattcaccg aaaaaagcca taccaatact aactccatac
    18961 aagcccccca caagatttcc attcttccag acttcgaccg aatgagcgta accaatttca
    19021 tgcaaccttg tgtaggcatc tacaatgtta ggaaaaatcc aggtaccagg ctgcccagct
    19081 cgcggcattg cagcgcaatt ttttattact tcttgaaaat tattatcgag cgtgagtgca
    19141 tacccagaat tcggctttaa tgctttttta agggcctttt gagctgattc cgtgacatgc
    19201 aaaaatccgc gcagaatgat aaaacgagga tctgggctgc accactggat gaaagcagga
    19261 ttggaaggcc aagggaagat tccttgacgg tatgcagaga tcaaagctcc tggcgaaaga
    19321 tttcctccgt aacatactac accattcggt gttgcacttt ccgctggcgg aaatcgatat
    19381 tcctgctcct catctagtct atctggcatt acaatccatc gcatcaaggc agctcccagc
    19441 ttttagtcct tttgaggcga aatatcgaat acaatctttt cgttctctag gcgagccatt
    19501 gccattccgc catgctcgag cttgccaaag agcacttcgt ccacgaatac agtttttatc
    19561 tggtcttcaa ccaggcgact tatatttcgt gctccgaatt cacgggagta acccttgatt
    19621 gcaagatatc ggattacttc atcttcaacc tttagggtca cattcttttc ggccaattgc
    19681 atagcgaatt catcgactgc cttcttgaca atacgctcga tgatctccat cggtagattg
    19741 ttgaaatgca ctactgcatc gagcctgttg cgaaattcag ggctaaaggc tcgttccacc
    19801 gcctcgtcga gggctgaaga gctaacctgc tggtcgccaa aacctattag tggctttcca
    19861 atatcacggg cgcctgcgtt gctcgtcatg atgagaatga cattacgaaa atcggctttt
    19921 ctgccttggt tatccgttag ggtcgcataa tccattatct ggaggagaat attgtagatg
    19981 tcgggatggg ctttctcgat ttcatcgaga agaacaacag cattaggcgt tttcctaatt
    20041 gcatcggtta aaagtcctcc ttcttcataa ccgacatatc caggtggaga accgattagc
    20101 ctcgataccg tatgtttttc ctggtactcg ctcatatcga aacgatgcag agttattcca
    20161 agatgtttcg atagctggcg agccaactca gtctttccaa caccggtggg acccacaaag
    20221 agaaagtttg caattggttt atcgggcgct ctaaagccgg ctcgcgagcg tttgactgcc
    20281 ttcacaacgg cttcgatggc ctgatcctgt ccaaaaactt cctgtttgag agaatcttcg
    20341 agagtagcaa gtttgtcttt ttcggaggaa gaaaccgttc tctcgggaat cctggcgatt
    20401 ttagcgatta ctacttcaat atcgtgaggg ctgatgtcga ctgtggcggt atctgtttca
    20461 tccttctcct tatcggcacg aatgcgagcc agagcaccag cttcatctat gacgtcgatc
    20521 gccttatcgg gtaattttcg ctcggttata aattgagcgg caagctttac agctatttca
    20581 agggtttcat cgctgtattg cacattgtgg tactcttcgt attttggtcg aaggccctta
    20641 agtatctcaa cggtctcggg aattgtgggt tctactatat cgatcttctg aaagcgccga
    20701 gagagcgcac ggtctttttc gaagatcttg ttatactcat cgtaggtcgt agatccaata
    20761 cagcgcagct ttcccgaagt gagagcaggc tttagcagat tagaagcgtc cagagtactt
    20821 ccagtaactg caccagctcc gattatcgtg tgtatttcat ctataaaaag aatggctttt
    20881 tctttcttga gcagcaaatc tatgactttt ttgacacgtt cctcaaaatc gccgcggaat
    20941 tttgtaccag caagaagcgc tcccatgtcg agggaccaaa tcgtataacc ttttagctta
    21001 ggaggcacgt taccagcagc gattcgttgc gcaagacctt cggtaatagc tgtcttacca
    21061 acacccgcat cgcccacatg tatgggattg ttttttagcc ttcgtgagag aacttgtata
    21121 gtgcgctcaa tttctgcctc tcggccaatg acgggctcga gttttccagc ggctgcaagg
    21181 gcggttaact cggttgcaaa gcgctctaat gttccaggtc gaagagtacg tttatctctt
    21241 gcaccttctt ccgctctgtc ttcagcctcc tcgtcggaaa gagcatcgct cgatatatcg
    21301 tcgtcgttta tgccatgaga aagtacctcg aggagttgaa gcctttttat gccgagctta
    21361 cgcatgagat atcctgcgta tgtgcgttct tcatcatata gagagaccac aagatccgaa
    21421 atctgaacct cttccttgcc tgccgattgc gactgcatta cagcgcgctc tataactgct
    21481 tgaaaacctg cgctctgaat aggttctgca ttgcggacaa tgggcatttt ttgctcaaag
    21541 tacgcctcga tgcttcgcct catctgttcc agatccgcgt cacagctttc aaatatagcg
    21601 cgtacctttt caaacgagag gcttgcaaag agtatatgtt ccggcatgag atattcatga
    21661 ttccggagtt ttgcttcatt gtatgcggca ttaaatatcg cctgcacctc agaacttact
    21721 ttcatgtttt ctccattgcg cagcgaagcg ggaaaccccg ctgtctggcc attgcatgta
    21781 cctgttgtac ttttgtaatg gcgatgtcat atgggtaaac ccctgctaga cctcgtcctt
    21841 ttctatggac ctccatcgtc aacttagtag cttccatcag attcttgtgg aacaccttca
    21901 taagaattga tatcacaaac tcgatcgtag taaaatcatc gtttatgaga tatacacgat
    21961 attcttcggg ctcccttgct tcgatttctt ccctcgtcag ttcttgagta tctatcgact
    22021 gttcgactct agttcccata catataaaaa atactcatag taacagactt taacaagtca
    22081 cccgcatgaa attcacagct agtctacact tgacatagag catctaaata tgaagaataa
    22141 taaaaaatgc gttgatggag aagagtagct ggcgaacctc gagcatagag agggattccg
    22201 tattagcaag tacgggtgga agaatcctgc cgatacccag cgaaaaccac tccggagtag
    22261 tctgagtgaa cctgattttt tcagcagtaa ttcagatcgc atgccaggcg ttattggcga
    22321 agagcgcggc ctctgtcgtg gggagccgaa ataaggtggt accgcgggtt acggggagtg
    22381 ttgcaactcc acggaattcg tccttatatg aagaatttca tgcaaggacg tatttcgtgg
    22441 agttttttta tatgctcaat gatagaaagg agagctaata tggatatcga aaagattagg
    22501 cattctcttg ctcatgtaat ggcagaggca gtacagcatc tttttccagg ggttaaattc
    22561 ggtataggcc ctgcaataga tgatggattt tactatgatt ttctatttcc acaaccgata
    22621 accaacgatg atttgtctgc aatagaaaaa gagatgcggc gaattatcga aaaggatcag
    22681 gattttatcc gcacagaaat cagcaagcaa gaagcgcaag agctcttcag ggatcaacct
    22741 ttcaaactcg aattgatcga aggcttggaa gaaggcaaaa tttcgatata caagcagggc
    22801 gattttgtgg atctatgccg aggaccgcat gttgaatcca caaaagaact aaaaccaagt
    22861 gcctttaagc tcaagactat tgccggcgct tattggcgag gagatgaatc gaagccaatg
    22921 ttgacgcgaa tctatgccta tgcctttgaa tcgaaagcta agctagagga ttacctccat
    22981 atgctggaag aggcggagcg acgagaccat cgcaaattgg gcaaagaact agatctcttt
    23041 tctacgcacg aagaagcagg tcctgggctc atatattggc accccaaggg cggccgtttc
    23101 cgtgtcgaac tggaaaattg gtggcgagat gaacattata aaaatggtta tgagatcctt
    23161 tttacgcctc atataggcaa gagttggctc tgggagacca gtggtcatct gggcttctac
    23221 aaagcaaaca tgtatgcccc aatgaaaata gatgaggatg agtacttcat aaagcctatg
    23281 aattgcccat ttcatatcat gatttacaaa acttccagcc actcttaccg tgaccttccg
    23341 cttcgctggg ctgagcttgg aactgtctat cgctatgagc gctcgggggt tcttcatggg
    23401 cttatgaggg ttcgaggatt tactcaggat gatgcgcaca tcatctgcac tccagatcaa
    23461 atcgaggatg agatactcga ggtgctgcga tttagcttat cgatgtggaa aaccttgggt
    23521 ttcaaggaaa taaaagcata tcttgcaaca cggcctgccg attctgtcgg agaaccagag
    23581 cgctgggatc aagctcttgt gtcgctcaag aaagcaatcg ataaagaagg attgctctat
    23641 gaaatcgatg aaggcggtgg tgcattctat gggccaaaga tagacttaaa agttaaagat
    23701 gcaataggtc gagagtggca gatgactacc attcagttcg atttcaatga gccagagcgt
    23761 tttgatatgg tttttatcga caaggatggc aaacaaaagc gtccatacat ggttcatcga
    23821 gcgcttctag gatctctcga gcgctttttc ggggttttta tcgagcatta tgctggggcc
    23881 ttccctgtat ggctcatgcc agatcaagta gccgtaatac cagttgctcc tagtttcgat
    23941 gcatatgcca aaacagtagc taagtttttg caggagaagg gcattagagc taaggcaatg
    24001 ctctccgaag agcgaatgaa tgcaaagatt cgagacgccc agaatcagag gattccatat
    24061 atggctatcg ttggtcagcg cgagcaagaa ggcagcctag tgtcgcttag actgagaaca
    24121 ggagatcaag agagcggaat tcctctcgag gtatttgctg cacgcgtttt agaaaaaatc
    24181 gatatgaagt ctctggattt ataattacag ttctacttgc tgtggcattt ttctgttaga
    24241 atgaaagact aaggagcaga aaatggcaga cttacggaca tatctgatgg ggcttcagct
    24301 acagaatcca ataatcattg gtgcaagcgg acttaccagt atgcctgatg gtgtagaaaa
    24361 ggcagcacat tctggcgtag gggcaattgt gctcaaatcg ctatttgaag aacagatact
    24421 tgccgtgctt ggacaggaaa tggcggggct cgatctaaat ggctatccgg atgcagaagc
    24481 cttcatttct cgtacagctt gggaagagag cacagatgaa tatctaagac ttataagcga
    24541 atctaagaga aaggcaggcg gagtcccaat aatcgcaagc atcaattgtg ttggccaagg
    24601 cagatgggcg gatttcgcta gacgcataga agaagcagga gcggatgcgc tagagctcaa
    24661 tatagcctat gtgccctttt ctccgcagat ttcgggacaa gagatcgaaa aaaaagtgct
    24721 ctcgaccgtc aaggaaacca gatctgtcac aaagcttccg atcatggtaa agctgggcaa
    24781 ccattattcg agcctgccgc atctagtgca tgggctttca aaagagggcg caaatgccgt
    24841 tgttcttttt aatcggttct ttaggttcga cttcaatctg aatacgatga gactatctgg
    24901 agcacaaccg cttagttccg agaccgaata ccatgagagt ctgcgatgga catcgatcct
    24961 ctataagcgg gtaggtcttg agatagtttc ttctaccggg attcacaatg caaatgcagc
    25021 ggtaaaatgc ttacttgctg gagcaagtgc agttgaagta tgttctatta tatataaaaa
    25081 aggctggaaa atcgttgagg caatactcaa agatatgaat atccttatgg attcccttgg
    25141 ctattcgtct ctggacggac ttcaagggaa gctggctgca ataaatgccg atagaccaga
    25201 ggagtatcat cgtcttcaat atatcaaggc attgactgac atatactgat ggaaatgttc
    25261 gtccaagggg ttgcgcctta gatcttcggt gaattgtggc ttagattttc ggttctcttt
    25321 ttattgcacc tcttcagagc acatgatagt attagactgt atcccgccgg ctataattat
    25381 tcggcgacgc aatagacaaa tgacccagaa cattccaaaa gggtcaaatt gcaaaaggaa
    25441 ggagactatg aagctcaatc taagtctaga caatctatca gaggcttttc cggaggattt
    25501 gacaccggac cagcgagctc gtgcacaaac catttttctg aaaacgcttt cagaaaagac
    25561 tcatcgtttt tatggaggga aaatccagat agtacccaag gcaggtctct atggattcaa
    25621 ctggttcaac gtatggtaca cgccaggcgt ttcaaaggta tcgacaacta ttcgagacga
    25681 taatgatcaa tcatttgcgc tctcaaaccg aggaaatctt gtcgctgtcg tgtctgactc
    25741 tacgcgtgtt ttaggcgatg gcgattgtac gcctcctggt ggtctaggag taatggaagg
    25801 caaggctttc ctcatgaaat atttgggtgg cgtggatgca attgctcttt gcatcaattc
    25861 ccgaggtacc gatggcaaac ctaatcctga taagatcatc gattttgtta aaatgctaga
    25921 accctcagta ggagctgtaa atctcgagga tattagtcaa ccgaattgct tcaaagtgct
    25981 ggatgaactg agggaagcct gcgatatccc tgtatggcat gatgatgcgc agggcactgc
    26041 ttgtgtgact cttgctggcc ttctcaatgc gctcgagctt gcaggcaaga agcttagtga
    26101 agccaaaatt gtattgctgg gagcgggtgc ttctaatacg acaattgccc gactaatcct
    26161 tgccgatggt ggtagaggag aaaacatggt gattttcgat agtaagggtg gtctgcacag
    26221 gaatcgcaaa gatatcgaga ccgacaagag atactatcgg aaatgggagc tctgccttca
    26281 aactaatcct cgttgcatag agaccgaaga agaggcctta aagggcgccg atgtactaat
    26341 tgcactatcg acaccagggc cggatacggt caaacgcgaa tggattcgat caatggctcc
    26401 caaatccatt gtttttgcct gcgccaatcc agtacccgaa atctggcctt atgcagccaa
    26461 ggaagagggc gcttacatag ttgccacagg tcgaggcgat tttccaaatc aggtcaataa
    26521 ctccatctgc tttcctgggc ttttgaaagg cgctttactt gttcggtcga gaaaaataac
    26581 agacaatatg gcgatccgat gcgcccactc tatagccgat ttcgcaagat ctcgtggtat
    26641 tagcgctgac aatataattg caacaatgga ggagaccgaa gtcttcgcgc gcgaggcagc
    26701 cgatgtagcc atggccgcga tagaagaagg agtagctagg cttcccgata cctgggacaa
    26761 tatttactcc aaggccaaag ccgatatttt agctgcacgt tctcttactc aagacttgat
    26821 gaaagatgga catatcgcag ctattccaga ggagatgcta caagaagcct tcgattttgc
    26881 ggtgtccgag gtaagaaaga aagcatagat cactgaattt gaggaaaccg aaatgggagt
    26941 ccaattgcta gctaggtcgc actaatactt gaatatacta gatcctataa attcacgcag
    27001 gattgaatcg ctgggcacat actgaggaga aataggcgaa atccgcgaga taaatgcctg
    27061 aatccggcga gcatccgtat attcggcatg atgtggctta actgttcgta gaagcggttc
    27121 ggcaaaaacc ttaagcaccc caagctcata gtcgagcgcg gaattgaatg ctatatctgc
    27181 gtcaccttgg aaagggaata tatagagtct ttcgcctctc tgtacagaag accacataga
    27241 aagcgtttcg cgagcatcgt gcccacgaaa attgtaatct cgcaccatgc gccgaagaag
    27301 gcgatagtct gtcgtccgga cacggttgtg atcgtccaca ttaagctgtg tcagtgctga
    27361 gacatatatc ttgagtttat tatctgcagg tatatgtggc gtaagccgct cattgagacc
    27421 atggatacct tctagcatga gtatttcatt ctctctgatg ctgataaaag taccagaagg
    27481 ctttcttata ccgcttttga aatcaaattc tggaagctcg atctctttgc cctcgaaaag
    27541 atccaaaagc tgtcgattga gaaattcgat atcgagagct tctaggcatt cgaaatctgg
    27601 tttaccgtcc ttatcgagag gtgtatgact acgatctacg aaatagttat cgagttcgat
    27661 atggataggt ttgaagccaa gagcccttag ctgtaccgat agttttttcg cagttgtggt
    27721 tttgcccgag ctcgaaggtc ccgctattag aatgactttt gtcttatcgg atttttcggc
    27781 aatcatatcc gcaatagcag caagtttttt attctggaga gcttcggcca tcagaatgaa
    27841 atcctgaatt cccccagatt gatttatctt attaagagca ccgatagatc ccgcattcag
    27901 cactttagct ttctgctcat attcttctgc tatgtcatag agcacaggta tgtctatgaa
    27961 agggtccatt tcataaggct tttttgtatg aggaaaacgg agcaacatcc cgcgacggta
    28021 aggcataagc tcccatacac ttaaaagtcc tgtgcgagga acaagaggcg ctatatgcag
    28081 atccttataa tcttgacact cattgagcaa gacaaaagga tcatttatat attccatcaa
    28141 agaaaaggtt tctgcttgat ttgattgttc aaagtattcg aagactgaat tccaggattg
    28201 cataataatt cgtatggtca gattttttgc tactatatcg cgcatataca agcagaggga
    28261 gttgagaagg tgtggcgaaa caggttcttc gtcatcgaag tagtgataat atccatttcc
    28321 tattgccatg ctcacgatga gtcgcctttt aggcacaata gcttttgcag ccatagcaag
    28381 gagaaaacat aaggaatggc gatatacttc ggcccccatt ggagaatcgg gatatatggg
    28441 agcaattttg cagtcggcgt tgaccggtgc cgaaagggcg gcaagctcat tgttaactag
    28501 agcagcaacc ggaggaaaag gatgtgtgga agttccccat tcttgttcca taccagattg
    28561 tatgaagatt tcctctgcat tcgatcccat tggtacagaa atttctttgt aaccatcgat
    28621 agttaaagtt atagtgctca tttatatatt ataagctatt actaatactt tgacgagctt
    28681 gttgcttttg tgtagaatta atctcatgtc aaaagtgaca atcaaggata tagctcaacg
    28741 ggcaggagta tcgaagacca ctgtttcctt tgcactaaac taccctgata gaatatcgaa
    28801 gaagacatat aacaagataa tggcgattat cgatgaactt ggatatgtgc caaacccctt
    28861 tgcgcgtagc ttgacgacaa aacgtattgg tgcaattggt cttcttctac ctcaaaaaat
    28921 aggagatatc tttgcaaatc cacatatgac tcaagttatc agcggaatag gagaggaatg
    28981 cgaacgaagg gaattctctc ttgctatctt gcctctaatc cggggcagaa taatcgaagc
    29041 tgcaagaaaa tcttacgttg atgggctcat cacaattgga gtcgggcctg gacacgaggt
    29101 tgtaagcttg ctcaaacaga atcacatccc ctttgtgacg attgatggcg aagaatcggc
    29161 ttcaataatc aatataggaa tcgatcaaaa gaatgctgca agagaattga tgcagcatgt
    29221 gttaaaaaaa ggccataagc gactcgctat actggcgcta gaacctgata caaagccgag
    29281 cggagccgag cttgcttcga ttgtcgtaca aaagcgtctc gaaggatttc aaattgcctt
    29341 gaaagatgct ggattgtcga ttactgattc tcgaatatct atccaaaact gcaagggctc
    29401 gatacagagt ggctatgaga gcgcttgcca tatcctttcg cttgatccac gaccaaccgc
    29461 gattgtcgcg atgtcagata tttcagcgat aggagctatg atcgctacaa aagaactagg
    29521 tctagagata ccagaagatg ttgctgtggc aggatttgat gatattcagg aaagcatgtt
    29581 ggtttctcct cgtctgacta ctattcacca gcccgaccga gacaagggag cagaagctgc
    29641 acgaatggta atgaacatgc tggatggttc ttccgggcaa cacataaaac tgccatatca
    29701 gcttatagta cgtgaatcga cctgatatat aaataaaatt gtcttgctat aaaaaaggga
    29761 ggaagaatga caaagataat tccagagcgt tcggatgttc ctgcagagca tagatggaac
    29821 ctggcaagtc tattttctac cgattccgaa tgggaaaaag aactagtggt cttcaatacg
    29881 atgattcatg atgcggaaaa gggcaagata tctcttggta cagatcggca ggcttttctc
    29941 aaaacactta aggactttga gcaatatcta attctagagg aaaggcttgg ctattatgca
    30001 gacctcaggg taacagaaaa tgaaggagat agcgtagcta gaggaatgtt tgagcgctat
    30061 gtaaatttat cgactcaggg tcaagccgct tggtcttggc ttagtcctgc tatccagatg
    30121 ctggatgaca attttatcga cagctgtctt aaagattctt cgtttgagga ttatagagtt
    30181 tttctttcga aattgcgtcg cttcaaaccg catgtgctga gcgatgctga ggagcgactc
    30241 ttggctcttc agagcgagcc agctcagact ccaaaggatg ctttcagcgt tctaaccaat
    30301 gtcgacttcg attttggtac aatcgagaca ccagaaggac caaaaccgct tacacaatca
    30361 acttttgcat actttatgcg tctgccggat aggtcggtac gaagaaaggc ctatctacaa
    30421 ttttatgctt cctttgaagc gcatcggcat acgcttgcga ctttgtatgc cggatctgta
    30481 aagctcgaca aatatatagc taaggtaaga aaatatccta gcgctagggc catggcactc
    30541 ttcccagaca atgttccgga ggctgtttat gataacctag tctctactgt aaatagaaac
    30601 atacccgctt tgcatcgata ttacgcgcta aggaaggaag tactcggttt ggatagtctg
    30661 aggcactatg atgtctatgt tcctcttgtc aaggaaatca agatcgagcg aagttacgaa
    30721 caagcagtgg atatagtgat cgaagcacta aagcccttgg gagaagagta tgtgaatacg
    30781 ctgcgcggag ggctattggg aggttgggtg gatcgctatg agaataaagg caaacgatct
    30841 ggggcttttt ccgcaggtag ttttttggga aatccttaca ttcttatgaa ctataaagac
    30901 gatgttctag acgatctatt tacgattgcc cacgagggtg gtcattcgat gcattcttgg
    30961 tattcagctc gcaacaatcc ttttatgtgt tacggatata cgatttttga agccgaagtt
    31021 gctagcactt tcaatgagca attagttttc tcttatctct atgagaacag caaagatgaa
    31081 agagagaagg cttatctgct tgcaaatcga atcgataaca ccctcggaac acttttccga
    31141 cagacaatgt ttgctgagtt tgaagagagg gttcatgaga ttgcagaatc aggagaggca
    31201 attactgtgg atgtgcttag aaaagagtat cggggcttgc tcgagaaata ttttgggcca
    31261 gaaatggtgt tcgaaaatgt ttcggatctt gaagggcttc gcataccaca tttctataat
    31321 gcattctatg tctacaagta tgcaactggg ttgtctgcgg gcattgctct atctgaaggc
    31381 gttttgaagg gcagagaaaa agaacgcgat gcttatctta attttctgag atctggagga
    31441 agccgttttc cgatagaatc tttagagctt gctggcgtgg acatgtcaac tcctgaaccc
    31501 atagaagccg cttgtaagcg ctttgccaaa gatgttgagg ctctttcggc tatattgaag
    31561 gcaaaatgat agagaaggct ctgttgtggg ggaagctgtt atagcgaagc ctgttatgga
    31621 gcagtttatt atagggcagc tctacatata tcccaagcgc gatagcaaga ttgagaccgc
    31681 tgcagaggca gcggtctctg ttttcaatat ccgatctcca agagagcaga gcaaatagcc
    31741 aacattaaga aaaatatcta tctcatgatt tgtccaacca cgctctgttc caatggctaa
    31801 tacaaccggc aatcccgaaa ggattatgtc gcctagaagc tgggcagacg gatcggggtg
    31861 gcagagtaac cttgtacatt tgctattgtc agtttcccgg gctgatatag gtgtttttgt
    31921 gtcggcatga tccatgctct ctgtgcgctc tgccttaatt agcgctctat ctagggtcca
    31981 agccagccca atttcaggca accttgggtt tcctgattgt tcggctcctt ctcgtgcgta
    32041 tattaaatat tccttttgct ggtaaaaatg agattgtgtg tatgattttt cccccagttc
    32101 ggtcggtaca aatacaacgc tttcgacacc aagtatggta agctctttta ctatgcgagc
    32161 cgcctgaatt ggtctcactg ttcccatgag gacacgaaga ggccttagtg gaggggcaga
    32221 ttcttcggct ataaaacgaa gcattatctc tgcatcgcta attgactcta agattgccca
    32281 accaatcaat ccatcaagac taccagcgcg cattcttccg ccaacctctt ttcttaggac
    32341 ttcgagaata tgctgagttc tccggtcttt tagagagaga aactgagttt cggaagatga
    32401 atcaagtatt aagatgttca ttcgagagag agaatttcgc gctcatagtc gaccccggga
    32461 acatcgaagc catgcgctct taaaaaatcg cttctgaagc cggctagatc agcaagttca
    32521 tggaggttcg attcatcgat cctagccagg cgtgaactga cttcgttctg aacagaagga
    32581 cccatctcga gatcatcgat ccgaattcgg ccttcgtcat ccgtaggaat ggctattcct
    32641 attttttggt ataggcgttc agagaaaagg cgcatcatct ggtcgaggca gtcttcatgt
    32701 atattgcgct ctttcatcac cttgaaaagt gtcgatatat atagcggaat tatcgggata
    32761 acggcgcttg ctcttgttac gactgccttg ttgatagaaa caaacgctcg aagtccgttc
    32821 tcttttccat agtccttttc gagcaggttt gctgtttttt ctagatggag tttcgcttga
    32881 ccgattgtac catcgcgata tatgggccag gaaaaaggag gccctatata ggagtatgca
    32941 agagtgatcg cttctggcgc gagcaaacct tcattctgga gagcatcgat ccatagcttc
    33001 caatcttcgc cgcccataac ttttacagta ttggcgatct cttcttctgt tgcaggttga
    33061 atcgttgcct cagaaaattt ttctgtaaac acatctattg tgcgacctgc gtattgtctg
    33121 cctataggtt ttattaccga acggtatagt actcctgtat cgggatctac tcgaacagag
    33181 cttgctagag agtatataac caaatcgtat ttgaaattat tttcaagtgc aatttctata
    33241 gctttctgtt tggcggcatg agaaaaggcg tctatttgag agaaggatgc tgcgaaaagg
    33301 ttattttcag tagcaagtct atcgaattct ttatttgcat accatcccgg agtcgctggc
    33361 ttggtggcgg aaggctcttt ctcgtaggaa aaacctatgg ttttggctcc atatccgaat
    33421 gcagcgacaa gtctcgatgc cagtccatat ccggtagaac agccaattat caaggcatta
    33481 tttggcttgg gagctgaacc atttatatca ctacttgcaa gccttttacg aaaagtcttt
    33541 gctcgcgctg cctgtaaacg cacttcctca cggcagccct cgggatgagc attgaggcaa
    33601 atattgtttc ggatcattgg ctttattatc attccggtac tcctgaagct atgcttatcc
    33661 attcggcttc gacagctatc gcatcgccta ccttacctat acctttttga tgaagataga
    33721 tagaggaggc acgcacattc ttgatctcga tcataaaagt atcgccaggg cgaaccgctt
    33781 gtctgaaacg agcttccttt atctttgcga acataaagag gcttttggga tagatgccca
    33841 tcaattttgc gcctactcct cctgcttgag ccatgctttc gatcaacaat actccaggca
    33901 cgataggaaa tgaaggaaaa tgtcctttga aaaaccaatc atcatcgtgg aatgcatgtt
    33961 gagctattat agtatcgcct tcgatataga catcatcgac gaacagaaat ggaggcctat
    34021 gaggaagata agattcaata ttcttcactg tctcaaatct ctccttatct cgtataagaa
    34081 ataatttctt tgcttaacaa tcttagccat ctgctttgaa tcgtgcaacg ggttttatgt
    34141 tcctactgcc ttgaatatga ttattccatt gtgtccgcca aagccaaggc tagctgacat
    34201 cgcagtcttt atcttgcgag cttgagcagt attaggaata taatctagat cgcaaccttg
    34261 ctctatatct gggttgtcta agtgaatggt aggaggaagg atacctgttt ctaatgactt
    34321 tatgcataca atggcttcga tcgctccagc agctgcgata cagtgccccg tcatgctctt
    34381 tgttgagctg attttaagcg acttgacaga ggaaccaaag gctttcttta gcatcttgct
    34441 ttccatgggg tcgttgaggt ctgtgcctgt accatgtgcg ttatagtatt cgatatcagt
    34501 tgcctgcaaa tcagcatctc ttagagcatg ctctatggct tttacaccaa agatgccttc
    34561 tggttgaggt gcggtcagat gataagcatc acaactgctg ccataacccg caaagattgc
    34621 tctgatcttc gcgtttcggg ctttggcgtg atcgagtctc tcaaggatta gaataccggc
    34681 tccttcgcct attacaaaac catctctatc gcggtcgaaa ggtctagatg ctagctctgg
    34741 tcggtcgtta taggatgtcg acagcgcttt gagcctacaa aaggaaccaa ctgcaaattc
    34801 agtaatagct gcctctgttc ctcctgcaat tacaatatcc actcgtccag cccgaacaag
    34861 gtctaaggct tcaccaagag catcggtacc tgaggcacaa gctgtaactt gcgtcaaagc
    34921 agggccatga acgccaaaat gcatggaaat gtttgcagca gcttcattcg ctatcatttg
    34981 aggaatggtc attggaggta tacgatcagg cccatcctgc agcattttgg catgagattc
    35041 ggtaaatatc tctatgcctc ctattccatt acccaagaca actgcgatgc gatcagggtc
    35101 tataccacct ttattttgca ggctctcatt ctgggcaaag tctggaagcc ccgcatccat
    35161 ccaagcctga gtggcagcgg cgactgcata ttgagaaaag agagccattt ttcgcgcttc
    35221 ttttcgatcg atccatacag atgggtcaaa attttttact tcggcggcaa tcttgctttc
    35281 tagcttcgat gcatcgaagt gtgtaatata acctacgccg gttaagccat tgatgcagtt
    35341 attccaaaac tcatcgatcg aattccctat aggacttata attccaagac ctgtcactgc
    35401 tacttcattt tgcattcttg ccttctcctt gtattaatga aaaagttttt atatatacaa
    35461 gtgacaattt gccaatagta tgcgatataa tagtcggatt ctgcaagacg aaatctctca
    35521 ccatcgaata accgcagctc cataagtaag acctgcacca aagccaacaa gcattatgag
    35581 atcattagtc ttcaaaagcc ccttttcgct catttctgtt agagcaatag gaatgctagc
    35641 actagaagtg tttgcatatt cttcgatgtt gacatagaat ttgtccaaag gaatcgataa
    35701 acgtttggca gcagcctgca cgattcgcgc atttgcctga tgtggcacta tccacgcaaa
    35761 atcttcgagc ttataagcgg ctttatgaag gagtctctgg attaaaacag tgatcgattt
    35821 gactgcaaag tcatagactt ttttcccatc catatagatg aatggtacga ttggttcttg
    35881 gcgctcaaaa gctttagttc tttcgggttg aacgagaaag agatcttttg ctccacctcc
    35941 ttcggctcca agaatgaatg aaagacatcc gcgagcggat tcatcgattc gggagagtac
    36001 cgcagcacct gcgccatcgc caaataagac tgctgtagag cgatcttccc agttgacaaa
    36061 tctactcaag ccttcagcgc ctataaccaa ggcatttctg ccgtgcgatg cttcaagcat
    36121 gcttgatgca atattgagcg catagatgaa tcccgtgcat ccagccgtga catcgaaagc
    36181 agcaacacca taagcgccga tcttatcctg aacaatacaa gcagtagccg gaaagccgag
    36241 atagtcgggc gttgctgttg ccacaatgat ataatctaat tcttgagggc cgatattcgc
    36301 cttatctaaa gcgctctttg cagcttctac tgccatatcg ctcgtttgga gtccatcagg
    36361 agcgatatga cgagcaccga taccagtatg agagcgtatc cactcgtctg tcgtatccat
    36421 gcgcatcgca agctcttcgt tagttatgcg aagaggtggt atataggccg aaattgatct
    36481 tacaacaata gacatacggc aatatctcct ctttctctac gatagatgct ctaaatgaat
    36541 catatagggc caatgttgtc aatatgacaa gaagagtaaa aatgtcaagg tattcttgga
    36601 tatgtcaact tctccgaata tctcgaataa tcttgagtat ataattagga cctaagctgg
    36661 tttatcgatc tagaatttag ctatgtttcc ctaagtctag actcctattt gccggaagcc
    36721 gaagaaattg aggctttctc tgttgctcca ggcagatgag cagcctccgc tgctaccatt
    36781 ggctcttgca atttcttggg tatagatgtt ccaccttcaa gaagttcagc gaattcggac
    36841 tcttctataa cttctttttc gagcaataca gaagatatat gatctagcaa gtactttttt
    36901 tctgtaagca tattgacaac atgttggtat cgctcattga tcatagaggc tatagtctcg
    36961 tcgatgtagc gttgcgtttc ttcactatat tcttttgttg caaatggatc agaagcttgc
    37021 tgagggctta atagtccgga agatcttctt gtcaaagcta cattcttgaa tttatcggac
    37081 attccgtagt cggtaatcat tctcttggct atttccgtcg cattggcgat atcattagaa
    37141 gctccagtgg aaatagactg aaataccagc tcttccgcag ctcgtccgcc aagaagcaca
    37201 tctatcttac caagcaattc ttcttcagtg attagatagc ggtcttcgag aggcatttga
    37261 agggtgtatc cgagtgctcc atagcctcga ggtacaatgg agatttttct tactggatcg
    37321 gcacctggag tgaaagcagc tgttatcgcg tggcctgtct catgaaccgc cactatctgc
    37381 ttttcctttg gatttatcat acgattcttc ttttctagac cagcaattac cttttcgatg
    37441 gcagcttgaa aatcttcttc tttgactttt ttccggccag cgcgaaccgc tagaagagcg
    37501 gcctcgttga caacattggc aagatcagca cctacgaatc ctggagtact acgagctatt
    37561 tttgataggt cgaccgaagg atccatttta atattcttag tatgaatctt gagaatttcc
    37621 tcgcgacctt tcatgtctgg cttatctacc aatacctgac gatcgaacct tccagctctc
    37681 aaaagggctg ggtctagcac atcaggccta tttgttgcag caagtattat gagaccgctt
    37741 gtcgaatcga aaccgtccat ctctactagc aactgattaa gcgtctgctc tcgttcatca
    37801 ttccctccga tgaacccgct cgctcgcgat tttccgagtg catctatttc atcgataaaa
    37861 atgatacaag gagccttttc tctggcttgc ttaaaaagat cgcggactcg ggcagccccg
    37921 actccaacaa acatttcgac aaaatcagca ccactcatct tgaagaaagg aacattggct
    37981 tcaccagcga ctgccctagc caacaaggtc tttccggttc ctggaggccc cacaaggagt
    38041 actcctttag gaatcttacc gccaatttct gtatattttt ggggattctt gagaaagtcg
    38101 acaacttcta ccaattcagc cttagcttca tcgactccag ctacatccgc gaatctcgtc
    38161 tttatgtcgc cttccgcgac gacggtagcc ttattttgac caaaagcgag tacattagaa
    38221 ttggatccca tgaatttgct catgatagca cgccagaaga aaatcattat ggcgaatgga
    38281 agtatccagt tgagcaaaag cgaaaggatt gtgcttcctc cacgtggaga tgccgaatat
    38341 acaacacctt tttcgtctag caaagatgtg aactcaggat cgctgaccgg aacggtctta
    38401 taaacaacgc ccggctttgc agatggcgga agaggagagg atggttctgc tggattgggt
    38461 tccgtatagc ctgtataata atttgcatcg atttcaaccc ttctgatctc gccggttctg
    38521 accttgtcct tgaatgtcga ataaggaaca agagtcgtat cgctcttgaa gagaaagtag
    38581 ttgaaaaagg agacagctag gagtgttatg agcacatatc ctaaggaaaa cctgaactga
    38641 aagccttctg gcattcctgg ctttttacga ttattagtat tcttatcggt ttttctttgc
    38701 tgatcgagtt tccaattatt cttgccttta gatggcatca ttttctccta taaaaagatt
    38761 catcgactat cttgattata gactatttgc atacttatcg taagcagaaa ccgtgactac
    38821 ttagtaatat actgattttc attctatatg gcgaataccc tattccccat ttaggtatga
    38881 agctttttag atgatttatc taaaagtcaa ttgatatgct tgaccaggag agattgtttt
    38941 tttatatttc aaatcggttc tctaccacaa atcatctcgg agcatttatt aggaggacat
    39001 atttatggcc gtaaaaccca ttggggacag agtgcttatc aaaatcaaag aaagcgaaga
    39061 caagacagca ggaggaataa taattccgca gactgctcaa gagaagaccc aaacaggtat
    39121 cgtcattgcc attggcaccg atgcggatgc tataaaggtc aaggtaggcg acgaggttat
    39181 gtacgataag tatgcaggca ctcaggtcaa gatcgagggt gtagaacacc ttatagtaaa
    39241 aatgtcagac attcttgcca tcctcgaata agaaagccta caccaatgat gaaagggggc
    39301 tgtccaataa gttgggcagc ctcttttttt caaagtgtac agcgatctgc tgcgatagtg
    39361 ctgaagtcct ttttcattct ctgaaaaggt cttaggcgtt gccaagcaga atagagacca
    39421 cttcttcctt agtcttggct tcgaggagcg cttggcgttt ttcttcgctg cgaaaaagca
    39481 tgcctacttc tgcaaggaat tgaagatgag ggccagtctt atcgatcggt gataatgtca
    39541 tgatgaaaat acgacacccc tttcgatcga gggcgtcaaa gtccacttct tgcttagata
    39601 tgccgacaca tgcgaccaac tcgtgtacag ctgttgtttt tccatgagga atagcaatac
    39661 catgtttcat gcccgtgctc atacgatact cgcgctcaag gacactttgg attgtttctt
    39721 ctttatccag tacctttccg ctattggatg caagatcgac aagttctctg atgatctctt
    39781 ctttgttcct gcctttaaga tccaatgaga tgagttcagg cttcaatatt tcctttaggt
    39841 ccattgttct atcctaattt gtggtcttga taccgtcaag tgcactttgc atcatgagtt
    39901 tatttgaggt catatgaaat tgcgtgcttt ctttacactg cgctgaccat tcagctcagc
    39961 aataagtgat aaggaaaata tcaaccaagc agtgtcgagt attgtcgcac agatagaaag
    40021 cggaagagca ataggtacca aaaggcttgc tccattttga aaaccctgtc caaaaagcaa
    40081 acaggcaaga gaaagcatga cgataatgtc gctacgtagg gcagaagcgc caactataac
    40141 cgaaagaggc agaagcgcaa gcagtaggat tatctggcct acagtgggat tattcctgga
    40201 ataggataaa aacattgcga tgaatgagca tgcaacaata ataacacttc ctactcgacc
    40261 actcacaagg acgagaggga atacaactgc attagtatcg cgctttatcc ccaagctttc
    40321 tgatacatgg cgaagtgctg tggcggaaga aagaaagata ctgcccgagg ctgcagctgc
    40381 aaggagcggt cctgacatag catagagaag tatataaggg ttggattttc ctccaagaat
    40441 tcgatagata agagggaaaa gcacaaagat cgagaaaact gtcagagcaa gcacgaacaa
    40501 gagaacactc cgatattcag aaggaatccc tgtatttcta agatttagaa agagatacac
    40561 agatatagga atgagcaaga ttcccagtat ttcagaaatg aaggaattta tcaggtaaga
    40621 tgctctagat attacatcca gcactggtag tattggcctc gacatgacgg gatcatgtga
    40681 tagcgccaaa ccaaaaaaga gagcaaaaag gccaattggc aggaaccact ttgaagagac
    40741 taaaaagact tcgaatatgc tcgtaggaaa tatggctcga acaaggtcca atgggtcaag
    40801 tgtctgtacg gttccacttt gatataccag aggcaatggt acgggcttga aaatcagaac
    40861 tatagctatg ccaataccag ccgcgactat taggctagca accgagagga taagggattg
    40921 agaaaagatt tttgcaaagc gatgggcttc tgacaatctg aaaactgaaa taggaatacc
    40981 tgctatgagg gttagaatga gcgcatagcc accaatcttc aaagctagct ctgcaagaaa
    41041 tgaaattgtc ggcgtaccat gaatcatact tggaggaagg gatatatata gaattgctcc
    41101 catggcaatt ccaagggcgt atttatgcca aagcttcatc ttgggaagca taactctatt
    41161 ggattgacag gtcaatattg agagctttct caaatcctcg aatgctggtt catgttataa
    41221 ctttgcattg acatatgaca ttgttttcaa gtaatgttca ttttattgtg cgacccagta
    41281 gctcagcagg atagagcgac tgcctcctaa gcagtaggtc ggccgttcga atcggccctg
    41341 ggccataagg aattacgacg aattatctaa tatgaggcta ttgcttttct aaaaacccgg
    41401 tagcgtttgt agatttttcc accaaagcgc tcagccaagg caggggtgtt aggattatct
    41461 tcagatgtaa gggataggtc tatccaggag tagccaaggc cttccaatcg ccggatcatt
    41521 tcatcaaaaa gaagagcggc tgcacctgct ccccaatgct ccgggggaac gaggacactc
    41581 ttaatggtta cacattcgta gcgtcggctt aagaagcgac ggataccccg catctccggt
    41641 gttctaggat gagagaatag actcatccta attaatccgc tcaaagccgc atagggagtt
    41701 cgaaagccat cgcattttat gagtacttcg ttgagatttg gcacggccgg aaagaatcca
    41761 accgttttgc cttccttcat tgcaaagagg accagcctag gatcggcgaa gctccggaag
    41821 ggttccaaaa gggctcgcac agctggcttc tcccaagcta tgaatcctgg caaatgagct
    41881 aggctcttat ttaaaagttc gagtacacag ttcacttcgt cttcgaagcg aaagaaattg
    41941 gcatttcgaa tagagtagcc tctgtgttct cgtgcagaag ctgccaagct ggctaactta
    42001 gcccgatcgg gtataggctt gtccaggcgt atttcaatgg cgatattgtc tcctcgcgct
    42061 ggtagaaaac ctaggatttc catggttgag aggtaatagg gcggggtatg gccgcacaga
    42121 aggacaggag ggcgatctcg accttctaca agaactccat aggcattttc gtagtctaga
    42181 ttgaagggtc catagagctc tgtaaggcct cgcgaatgcc cccattcttc aaccgtctct
    42241 ataagagccc gagcgacatc gagatcattg atgtactcga agaagccaaa aatgcagtgt
    42301 tttgttgcat cgcgcgtgtt cgcagaacag tcttcagccg cggctatagt gcctacgatt
    42361 ctgccttctt taatggcgag aaagagcttt gcttctcctc cgcgaagaaa gaactctcct
    42421 ctcttaggat ctaatatggc cttgcgctcc ggcagaattg gaggaaccca taatgggtca
    42481 ttgccatata ttgtccatgg aaaacgcaag aaccgctcga aatctctctt gttctcgaca
    42541 ggacagacct gtactttaga ttttccttgt atctctattt catccataga ttgtcctatt
    42601 cagttgtggg gggcttctgc aggatctatt tgtacatctg cgtaattcct tcggcgttca
    42661 tcgagttcag ctgtaagtcg tttgtggtcc tcgcgcttca aaggatattt taaagcaaat
    42721 atagcgcctg atccaaggag tagagcaggt attatgctca tgactagtcg gatccctcga
    42781 attgcagatt ctgattgttt ttccgctctt ggagcatatc ctgcagctcc aagcatgagg
    42841 agcactaggg gcaaagctat ggaactggcc gctttttgtg caagggttat gagtgaatag
    42901 aacattccct catggcgttc tcctgtctca tattcatcta gttctacggc atctgggaga
    42961 atggaccagg gaatgacatg cgcagcccca acaccgattc ctgcagccac ggataagaca
    43021 gcgatcaccc ccatagaagt cgttgccccc aggaaggata gcaatcccaa acagattgcg
    43081 agaaatgccg ctccaactat ataggcaagt cttttgtccc aaaggccaga taagaagttc
    43141 cagagaggta tggatatgag ggctgtgaca aagataaggg ccattattaa atccatatag
    43201 gattctcgct ggattacatg tttgatgtaa tagacaatta gagcctgtag gagatctatg
    43261 cagacccagg ttgccaggaa gatcacggcg ccaaaaagaa aagcacgatt gccaaaagca
    43321 gccttaagag agcctttaag gttcggcacg gactcttttt ggaactcagg gcgctcatgg
    43381 actgcggcaa aacaaataaa gagaggcagc actgagataa tggcaacacc tactgcaaaa
    43441 accaggatcc tatctgcatt gtctggagag aagcttccaa taaatgccca aggaagcaca
    43501 aatacaatga ggcttccaat gatcgaaaag ctcatcctga aagaggtgag cgatgtccgc
    43561 tgatcataag atggagctat ctctggtgtc agggcgtaat aaggcatata gacgaaggtg
    43621 gctgcagtat caaagagcgc atatgctcct ccgtagtatg ctgccaataa agcaggatta
    43681 ttcaggggtg gccgcaacca aagaagcaga aaagttgctc caaaaggcaa ggcgccaaaa
    43741 aggaggtatg gccgtcttct gccccatcgg cttcgtgttc ggtctaccaa atgcccaatg
    43801 atagggtcgt ttatgtagtc tacgcttctg cctataaaga tcgctgttgc tacaagggtt
    43861 ggagccagcc caaccacgtc ggtcaggaaa atagagaggt aggcacctac catggttgtg
    43921 gtaatggaaa accccagatc ccccgagcca tacgcaagtt taaggcttag cccgagttca
    43981 tctttcatat aaagaatcat aagtcgaggc acataagcgg tcaagattcc actttttcct
    44041 tcgataatct ttgcattttg tgttcgtgaa aacatgtcta tgcctgccta aaatccaaaa
    44101 aaataggcgc agtacagatt tatagtgctt gaataagatt cccttcttga caatccagat
    44161 ttttgtggta ccattattaa accggttaag caatcaaact taatcgaaaa gtttgaagga
    44221 ggaatgaaat atgaaacggt ttatgttggc attcatggcg ctattgacag taactgctgc
    44281 atttggcgct ggaaagctta ccttgatgca gaataagccc gagatcgatg cgcagcttaa
    44341 agcctatgct gcgacatggg gccagaaaaa tggcgtaact gttgtgatca aatcgatcgg
    44401 cgggacatct ggcggtatgg gtcctcaact caaagcggac tatgcagcag gagatatgcc
    44461 agatatcttc gctttcgatg gcctggaagc ctataaggag tgggaaggaa tcatcttaga
    44521 cctctcgaat gagccctggg tttccaagac ttctgttccc tttaaatata atggaaaggt
    44581 ctatggattt cctgtagcaa tcgaaggatg gggattggcc tacaatgccg acctcctttc
    44641 taaggctggc atagatccaa agactctgaa caatttcgat gcatataaga aggcctttga
    44701 aaagctcgat tctatgaaga aggatctagg catcaattcg gttgtctcca tggctgcctc
    44761 catcgagatg ggatgggtta ctgctcatca caacttcaac tctttgttat ccaatggctt
    44821 accttatggc gacctctctg tgacgaacga tctgttagct ggcaaagtcg atgcgcaaag
    44881 acttcaggag tatgcagact gggtagagct tctattcaaa tattcagata aagcgatcct
    44941 ccttactggc aattatgatg ctcaagttgg agcctttgca accggcaagg ctgtcttctt
    45001 gcaccagggt aactgggtag atcccaacct gaaggatgca aataccacat tcaaaatggc
    45061 ttttgctcct catggatcaa tgaaaaaagt taccgatggt atctttgtct cagctccatc
    45121 ttattatgcg atcaacaagg aatcgaagaa catcacgctt gccaaaaaat ttcttaacga
    45181 ccttgtcatg actccagaag gaaataatta catggtcaag gaagcaggta tgattccagc
    45241 cttctcgaat gttactctca gtcccgaagg ccagctctcc aaatctgttc agcagtggtc
    45301 agctgctggc aaggtctaca gctggaatca gtattacttc tcgggagatt ttagggacaa
    45361 ggtgcttacg ccaatctaca atcagtttgc agctggcagc ataagcaagg ctcaattcat
    45421 cgatttaatg accaaagcat tcaaggataa tgccaagaaa taattttaca ttatagaata
    45481 ctttttcagc ggagctgtcc gcgaagtgtg cttgctttgg gccgcctgca ttgctaatgt
    45541 tgatgcaggc ggttttattt tgaaatcctc aatagaatag gggagcaagt atgacaaaaa
    45601 agacggaatc ttcattggtg ttttggatat ttcttttgcc tgtgctattt gcgttcatca
    45661 tggtcatggt aattccattt gcgttaggat cttattatgc gtttaccaat tggacctcat
    45721 cggcgagggt agacagtagc ctcaagtttg taggtttcga gaatttttct aatagtttga
    45781 aagatccatc ttttttgtac tctttcggta tcacctttgt atatacaatt ttaaacatga
    45841 tcgtcatcaa tatcgtagct ttcgtgctgg ctctgttcgt aacgggcgat cttcggttca
    45901 agaatctata tcgagttggt tttttcgtcc ctaatctcat aggcggaatc attctaggct
    45961 atatttggca gtttattttc aataatgctc ttccttcgct tggttccatc gtacctgctc
    46021 tcgatttctt atccgatcca gccaatttga tgttaagcaa gcctaatact gctatcgcgg
    46081 caatgatcat cgtaggcaca tggcagtacg cgggctacat aatgatgatc tatgttgcgg
    46141 cgatcgaaaa tattccccag gaactcatcg aggcttcaag aatagatggg gcttcaactt
    46201 gggttcgtct caaatctatc acaatacctc tttgtgctca ggcttttacg gttactatgt
    46261 ttcttacgct cgtgaattcc ttcaagcaat tcgatgtcaa tgtttcgctg acgagcggcg
    46321 gaccttctac tatattgatg ggtcaaccta tttttggaac ggagcttctc gctctcaaca
    46381 tatataatac agcttttatt tcgaacaagc tatccgaagc acaagcaagg gcgtttatct
    46441 ttttccttat actagcgatc atttcgatca tacaggtgaa tgtgaacaag aaaaaggaga
    46501 ttgagctatg aaaagcgcta ggattaggaa accaacatct ctccttgccg aaatagtcac
    46561 tgccatactt tttatagtat ttttatttcc ctttttcctc gtgctcatca attcggctaa
    46621 gacatctttc gaggttactc aatatccctt agcctggcca aaaagatggg ggactattgt
    46681 cgataatatc gttaaaatct ggactagcga atcggttcgc tatcctagct cacttctttc
    46741 gagtgcgata ataacggctg tttctctaat tttgatcaat ttattctctg ctatggctgg
    46801 ttgggctctc gttaggacaa aaaccaaggt ttcttcattc atattcttta tttttgtcgc
    46861 ttctatggtg atccccttcc aaattgtcat gtttccgctt ctatcctggt ttcgcaccgt
    46921 taccgtggca acaggcataa gactattgcg tacatatcag ggaattattc taagttatat
    46981 cggctttggt gcacctcttt ctatatttat gtttcatggt ttcattaaaa gcattcctct
    47041 ggaattagag gaagctgcta ctatcgatgg gtgtcataag catcaaatct tttttaggat
    47101 tattttccct attctaaagc caatccaggc cacagtgctg gttctaaatg gtatctggat
    47161 ttggaacgat tatcttttac ctcttcttgt tctcggcaaa ggtaatgatg taatgacggt
    47221 gcctttagct gtttccaatt ttgctggagc ttttgtcaaa caatgggatc tcattcttac
    47281 agctatactt atggcaatga ttcccgtaat tgtgttcttt ctcttcgctc agaaatacat
    47341 tgtcaaagga atgattgcag gcgcaataaa atagtgatcc taccgataga aagggttaat
    47401 tctaaacgac atcgaagctt gcgctaagtt caggaatcga aacacgtcgc agcgcgatag
    47461 ttatgtttgt atctagcttt atgttgcgag atatacggat aggcatttcg atcccaagag
    47521 atggaatata gatctgagca ttgcctctat cgacttctag aaccgtcgct tggcccatcc
    47581 aatctctatg tttactgagg taaaccattg tccaatgaag gcgggaatct cgttcagctt
    47641 ggcgattttt ggctacttgc tgactataga ggtagagtct ttcatccagc atttcgcggc
    47701 taagccctat tgctgcttct ataaaagttt cttcgtctga tctgtggctg gacttttgtc
    47761 gggcaagctc cgcatgtatc tggtaatgtg ccaacatatc ctgatatctc cgcagcgggc
    47821 tcgtcacttg agaatagaat gataggccaa ggccagcatg agcagcgcag gtggaactca
    47881 caaaagctgt acgcataacc ttgcggcgcg catagttttc tgcaagactt tccgatggtc
    47941 ctgcaccaca gccagctaaa tcagaggaaa taggtggctc ctgcgaaaca tatggaaatg
    48001 aaataccagc cgaataagcc catcgcgctg cagcctctcc tgccaaaatc atgagttctt
    48061 gtactagctt tgccgatcgg gttgctggaa caggcagaaa ctcaacatcg tcatctatag
    48121 ctctaatcga tatttcaggg tattcaatat ctacagcgcc ttgatcttgc ctaagctttt
    48181 ttcttgcttc tgcaatttga tttagatttt ttaatactgt atcggtttcg agcaaaacat
    48241 cggcttcttc gtaagtaagc cttgttactc ttataatact tggtacgatc gttatttctt
    48301 cgatttttcc atttttctgc atggtaattc cgaatgagat cgccctggat tctggcaaaa
    48361 tgccaagccc aaaagcctcg atcaatgaat ggtgaagcat aggtatcgtt ttttctggca
    48421 aatacaaggt agaagccctt gtgcgcgcat cttcatcttc agcagaaata ggagcgatag
    48481 cggaaactgg atcggaaacg tgtacccaaa tcctgttgcc ttctatagag atagcatcgt
    48541 caggatcttt agaccatgca ttatcgatag caaaggaagg aagatgtctc aagtccaatc
    48601 gctccaaggc caattcaggt cgttcctgga tattctttgg ctctgaagga atggataata
    48661 tgcatccatt tcgctctggc caaggattaa gactaggctt ccaaaacccg gttgcaagca
    48721 gagcttcatg gactgcttgc ggagtcaatc tgattccaag atcgcgggca atcggactgt
    48781 ccgatgtaag gccacatgca aacctgctca attcatccac aaaaggttta aaattctcat
    48841 ttttctcaat aaagaaagga tcgcgctttt tccatgctaa gctaaacgca tcgacgaaag
    48901 ctgttctagc ttcggcaatt tcttttctta ttctttcctt ttcgaggagt tttagctgtt
    48961 cttctttgct tcgaattcta aagccattat caagtatttc tatggcaggg ttttcaagaa
    49021 gcgtatacca agctata
//
LOCUS       DAOSOB010000007        48385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_791_length_48385_cov_62.084254, whole genome shotgun sequence.
ACCESSION   DAOSOB010000007 DAOSOB010000000
VERSION     DAOSOB010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 48385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48385
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_791_length_48385_cov_62.084254"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..481
                     /locus_tag="PLS88_01455"
     CDS             182..481
                     /locus_tag="PLS88_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU06058.1"
                     /translation="MPSTGKPMTKVEIIDALAKSTGFTKKDINLVLDELAKLAYSEAK
                     KNKKFTIPGIAIMKVVHRKAKVGRNPATGEQITIPARNVIKITVAKPCKDAVLGK"
     gene            complement(590..1948)
                     /locus_tag="PLS88_01460"
     CDS             complement(590..1948)
                     /locus_tag="PLS88_01460"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOU06059.1"
                     /translation="MSETTVTMEKIVSLAKRRGFVFQSSEIYGGLSGAWDYGPQGIEL
                     KNRIARFWWKEMTQMHDNIVGLDAAILMHPRVWEASGHVENFTDPLVDCKKCKGRFRA
                     DQIPPENLAEKKCPDCGGELTDTRKFNLMFKTNLGPVEDESGLVYLRPETAQGIYVNF
                     KNVQQSNRMKIPFGIAQIGKAFRNEIVTKNFIFRTCEFEQMEMQFFVKPGTDEDWFEY
                     WRAERWSYYKKMGVNMKKLRWHRHGADELAHYAKDAYDIEYEFPMGFKELEGVHNRTD
                     YDLSRHQQYSGKDLQYIDQDSGNERYIPYIIETSAGLTRQLLMLLCDSYEEQKISDKG
                     NEDDWRTVLHFHPILAPITVAVLPLMKKDGLAELAQNIRIELKDDFVTDYDQAGAIGR
                     RYRRQDEIGTPFCITIDYQTKEDNSVTVRDRDSMLQERVSRANLTSYIQQKIKSYNTA
                     SI"
     gene            complement(2003..3733)
                     /locus_tag="PLS88_01465"
     CDS             complement(2003..3733)
                     /locus_tag="PLS88_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOU06060.1"
                     /translation="MSELVEKPEYLDFIREAVAEDLRTGRYKEVRTRFPPEPNGWLHI
                     GHAKALFVDFGVAQDFGGKCNLRMDDTNPEKEDMEYVEAIKRDIKWLGFDWEDRLFFA
                     SDYYEKLYELACKLIKKGLAYVDDLDEEQIKEFRGTNVPDKNNITYTPPGRNSPWRDR
                     SPEENLDLFERMRVGEFADGSKTLRAKIDMAHPNLLMRDPVMYRIRRIPHYRTGTKWC
                     IYPMYDFAHPLSDAIEGITHSLCSLEYEIHRPLYEWFIQNCEVFPSRQIEFARLNLSH
                     TVLSKRWLLQLVREKKVKGWDDPRMPTIAGMRRRGYPAAGIRDFLGRIGIAKTDSLVD
                     IQLLEHCIREELNTHANRYMAVINPVKLVIENWPEGKIEWLEAVNNPEDPSAGIRKIP
                     FSSTLYIDRDDFREVPPPKYYRLYPGNQVRLRYGYIVTCTGFEKNPTSGEIEKIRCVY
                     DPNTRGGDAPDNRKVKGTIQWVCAIHAIPIEARLYDHLFEAERPMEVPEGKTFFDNLS
                     DHSLTIESRAVAEPAVVEIEKGQTVQFERIGYFCKDPESTSSLAVFNRTVTLRDTWAK
                     IEKKFQEKPH"
     gene            complement(3802..5271)
                     /gene="gltX"
                     /locus_tag="PLS88_01470"
     CDS             complement(3802..5271)
                     /gene="gltX"
                     /locus_tag="PLS88_01470"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOU06061.1"
                     /translation="MSVRVRYAPSPTGNQHIGGVRTALINYLFAKSQGGTFILRLEDT
                     DRTRYSQEYVQNLYETFRWLGFYWDEGPDVGGSKGPYVQSERIDLYRRYAEELVFMDK
                     AYYCFCDSERLEKLRQEQVAQKKDEIGYDRHCRYIAEEERARNLAEARPYVIRLKIPL
                     HGITVFHDALLGQIEWKNEDISPDPVLLKSDGFPTYHLANVIDDHLMGITHIMRAQEW
                     IPSAPMHKIMYDALGWEMPELCHLPMVLGPDGHKLSKRHGATAVNEFKKAGYLPEALI
                     NYIALLGCSYEDGRDIYSLDELVRLFRIDRLNKAPAVFDYQKLEWFNGQYIRMKSDKE
                     LAELIRPYLISAGLRKEEDPQKDQMELAAIPFIKERLKLLGDSPAIMSYLYKRLDMPA
                     AEEFVPKKASIDEAIMYLEECRSILESSNLDDLPAIEEKFRERATVIGKKLGDILMPL
                     RVAITYSRVSPPLFESMRILGKDECIKRIEAAVAYLRGA"
     gene            5782..6270
                     /locus_tag="PLS88_01475"
     CDS             5782..6270
                     /locus_tag="PLS88_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOU06062.1"
                     /translation="MEYLFNFKKIGYLQGKRPKGCILCLVRDDSKEVEKLVVHETEHF
                     IVSINLYPYNPGHLIVFPKRHIVDIREYSDEEEKELDKLTKTCLEALDKYASPSAYNI
                     GYNMGLSAGASIEHLHMHIIPRYPREIGIVELIAGSRVLVQDPKDTLQQLKEIFSNLA
                     IL"
     gene            complement(6256..6906)
                     /locus_tag="PLS88_01480"
     CDS             complement(6256..6906)
                     /locus_tag="PLS88_01480"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOU06063.1"
                     /translation="MKALIVTGGDNPPSSVIRHLSENSDIVIAADAGLEICIKAGIEP
                     HIAIGDFDSVDPAILSQLSTEKIFRFPEEKDYSDTELAIDAAHRYGAKDIILAGGGAG
                     RLDHLLAIRALFERKDPIKEWHTAHESAFLVDRGFKLQASMPINSVVSVFPLSEGALG
                     MRSNGLKWPLDGLKWDCSEFGLSNKTISEEIEIYSGEKPILVILPLDLKISISQDC"
     gene            6962..8335
                     /locus_tag="PLS88_01485"
     CDS             6962..8335
                     /locus_tag="PLS88_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU06064.1"
                     /translation="MIIPSIDPEVRRIADRIIQSGKKAFLVGGAVRDSFLKRPVIDYD
                     IATDATPEECMMLFPFAIPTGIKHGTITIISRSRRYKIEITTLRKEADYSDGRHPDSV
                     TFVDDITQDLSRRDFTINAMAIDLVRNIFIDPFDGKKDLKNKIIRAVGNPIERFQEDG
                     LRTIRAIRFSAQLEFAIEPNTLAAITSCSSNLSRVSAERIRDEFSKIILTAKPSIGLR
                     ILSETGLIEEIIPELKDCKGIPQPNHCEKDVYEHLLSTLDAVIPDNLEEERLLTLRLA
                     ALLHDIGKPVCYRTENETVSFYRHEIESERIARQALMRLKYPNRVIDKVCHLVRYHMF
                     DYEPNWTDAAIRRFIARVGFSTIPDLFALRLADTTATTGKPISWPALSEFKARIDRIL
                     EEKQALSLKDLAINGENLARIGIPRGKQMGEVLSELLDTVLEDPALNTKEKLLEIAKA
                     KYFGRFD"
     gene            complement(8347..9156)
                     /gene="rnc"
                     /locus_tag="PLS88_01490"
     CDS             complement(8347..9156)
                     /gene="rnc"
                     /locus_tag="PLS88_01490"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOU06065.1"
                     /translation="MKQKDHSWLSSGQEISPSRYSALLFFQEATGISFHDITLLDIAL
                     THSSSVNENSSNRQDNERLEFLGDSVLSLCISHILYENLPDKNEGELAHMKNVLVSET
                     SLASIAMSLNIGEFLVLGKGEEMSGGRMKPAILADATEALLGAYYLDMGIIPTKEFIS
                     RLFKSKLEELRKTPSKDFKSIIQEYAQKLGIEIPSYLVIKIEGPEHARHFFVSCVIDG
                     ETFGPLEGMSKKEAEQRVAQHIFEVLHNRNAETARLLDLVAGGSSEADKDI"
     gene            complement(9173..9415)
                     /gene="acpP"
                     /locus_tag="PLS88_01495"
     CDS             complement(9173..9415)
                     /gene="acpP"
                     /locus_tag="PLS88_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOU06066.1"
                     /translation="MDEQELFEKIKKIIADKLEVDESKITMDASFRQDLGADSLDTYE
                     LVYGIEEELSIQIPDEKANEFETVKDAFEFIKTQVK"
     gene            complement(9427..9612)
                     /gene="rpmF"
                     /locus_tag="PLS88_01500"
     CDS             complement(9427..9612)
                     /gene="rpmF"
                     /locus_tag="PLS88_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOU06067.1"
                     /translation="MAVPKFRTSKARTRRRRSINMKLNPPAVVHCSNCGNPVMLHRVC
                     PKCGFYRGRQVITPESK"
     gene            complement(9732..10229)
                     /gene="coaD"
                     /locus_tag="PLS88_01505"
     CDS             complement(9732..10229)
                     /gene="coaD"
                     /locus_tag="PLS88_01505"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOU06068.1"
                     /translation="MPNAVFAGSFDPPTSGHLDVIKSALSIFDGLYVVVAINPEKDGL
                     FTIDERIEMLNAMNRFGQRMKVCKWDGLVTDFASQHGCTVLVRGLRNAAELPYESTMA
                     YMNQRLDPSIKTVFFLYDAEHVDISSTLVRDLVSHSRLPNDIVPEEVEMVLRQILQRR
                     GQPLS"
     gene            10327..12030
                     /gene="lnt"
                     /locus_tag="PLS88_01510"
     CDS             10327..12030
                     /gene="lnt"
                     /locus_tag="PLS88_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOU06069.1"
                     /translation="MNELAKGLHRHNILKPENLSSFSTPFWIRLLWAFLAALFAALAQ
                     PNELFLYGNWFFGLFCLVPLYMALVDTEKLEEASLIGALFGALYHALTSYWLFFYKNF
                     AFWTLGTTTIAYAVIYGVALMYGCFFLHHTDGCRPLAFALAWAALEFAKSTGFLGYPW
                     GLLAYSFTGLPIMLQTADLWGVYGISAFLALCSSATSELILQKTRKMSPLKSCFRWPT
                     IAGTWAIILLLYGALTISRPIPIRATLRLLLCQQNTDPWISGENEALYANIKVAKEAL
                     KSIQEEKGAMPDLIVFSETSLRRPYKDFQSWFEHNPPEEPLLPFLRNANIPLLTGLPV
                     VWNWDTNEASNSVGIIYPTGKIGETYAKIHPVPFAEAIPFWEFSWFRSFIQNVIGLES
                     GWVMGNEEVIFALSPKNRDDGMNGMNTLPTIRFATPICFEDAFSELCRDFIHRGADLL
                     INLTNDSWSRTKSAQIQHYAVARLRAIETRRALVRSTNSGVTCVIGSDGRNIAELPQF
                     INASMVVDVPIYESRFTFYLNFGDWFAWVCADFVGLLFIWAILDTKRKNYVIQSNINH
                     L"
     gene            12061..12468
                     /locus_tag="PLS88_01515"
     CDS             12061..12468
                     /locus_tag="PLS88_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06070.1"
                     /translation="MSISSYFDAEDLEMLEDVSDIVRYREHPAFDAVPFVGSIKKHPY
                     DKEKFLILLLHEQKNMPWFKEGEIIEIKTRDVQALDELPSTIDLNGTAISTFRIWIRR
                     RAIAVRFEPFEVNDKEYRPFDTHSISRLLSKRD"
     gene            12468..13340
                     /gene="amrS"
                     /locus_tag="PLS88_01520"
     CDS             12468..13340
                     /gene="amrS"
                     /locus_tag="PLS88_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOU06071.1"
                     /translation="MIDNGLSADRARYWRSLDNSRIQCILCAHACIIQNETRGHCNAR
                     QNRSGKMILPFYAWISSIAIDPIEKKPLKRFMPGTSTFSVGFWNCTMDCPFCQNWEIA
                     HPPPAYIQKQIIEPDRLIELALASGCPSISFTYSEPLLHIEYVIEAMQIAHSRGLKTI
                     LVTNGNALDEPARHILTHTDAANVDLKTADPLIYRKLLGGEIKVIKNFIKVAFELCHI
                     EVTSLAVPGVLDSIAQIEEISSFIASLSTEIPLHITRYHPAWKYTKPPLSPDTIHEMA
                     ESARNYLKNVYSYW"
     gene            13399..13471
                     /locus_tag="PLS88_01525"
     tRNA            13399..13471
                     /locus_tag="PLS88_01525"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13432..13434,aa:His,seq:gtg)
     gene            13514..13587
                     /locus_tag="PLS88_01530"
     tRNA            13514..13587
                     /locus_tag="PLS88_01530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13548..13550,aa:Arg,seq:tcg)
     gene            13603..13675
                     /locus_tag="PLS88_01535"
     tRNA            13603..13675
                     /locus_tag="PLS88_01535"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13636..13638,aa:Lys,seq:ctt)
     gene            13755..13835
                     /locus_tag="PLS88_01540"
     tRNA            13755..13835
                     /locus_tag="PLS88_01540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13788..13790,aa:Leu,seq:tag)
     gene            13908..13979
                     /locus_tag="PLS88_01545"
     tRNA            13908..13979
                     /locus_tag="PLS88_01545"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13940..13942,aa:Gly,seq:gcc)
     gene            14093..15448
                     /gene="tig"
                     /locus_tag="PLS88_01550"
     CDS             14093..15448
                     /gene="tig"
                     /locus_tag="PLS88_01550"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOU06072.1"
                     /translation="MEISEKKIEELELSRMRMTITVPAQEVRSEYDTMMNEYAKQARI
                     DGFRKGHVPVPVLERKFGASLKTDAMARVIEKALETALKDVSRPPLAYEMPTLEDDPS
                     FELDSDFIFSVTYDTFPVFELPSLDGIEIEVPNVTITQEDLDRELENIRQRNAIVAEK
                     DGPSEIGDIVTASFVEIAEDGSEIPGTSRQDFSFELGKNLNIYKFDDEIIGLKAGDEK
                     IITKTFPDDFEFPEYAGKTATIKVLISKVKHQDLPDLDDELAQDVSEKYKTLDDLKEA
                     VRAHLMEDLEAKLRKTKENAIMDELLKRTNISIPRSMITAELAMRWESLKQQTGVDSD
                     EHLEMLLAMSGKTREMIFQEWEPLAEKALATNILLDKLIEAGSYSSSDEEVDAEIAKE
                     AIHSTMSPAEIKAEYEKHGTIKYIKEDIAMRKFFDNILSSATIKESAPMSYLDFMKGN
                     D"
     gene            15515..16126
                     /gene="clpP"
                     /locus_tag="PLS88_01555"
     CDS             15515..16126
                     /gene="clpP"
                     /locus_tag="PLS88_01555"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOU06073.1"
                     /translation="MSEYMSNLVPIVIEQTGIGERSYDIYSRLLKDRIVFIDGEISDI
                     IADLVIAQLLFLESQDPSRDISIYINSPGGLVTAGLAIYDTIQYIKPDVQTICIGQAS
                     SMAALLLACGTKGKRYALPSSRILIHQPWGGVSGQASDISLQAREIIRLKKLTIDYFA
                     LHSGQLIEKVAQDMERDFYMSSQEALEYGIVDTIMEPRKHGKE"
     gene            16113..17366
                     /gene="clpX"
                     /locus_tag="PLS88_01560"
     CDS             16113..17366
                     /gene="clpX"
                     /locus_tag="PLS88_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOU06074.1"
                     /translation="MARNKQFDLEQETFCSFCGKSSSYVRKMIAGPGVNICDECVRIC
                     SQILDEEEQRVTANFLTEVPKPKEIKEFLDMYVVGQEYAKRVLSVAVYNHYKRLVHKV
                     SAPSPDVEIEKSNVLLIGPTGSGKTLLARTLARKLKVPFAMADATTLTEAGYVGEDVE
                     NILLKLIQNAGGNIQAAERGIVYIDEIDKISRKGENPSITRDVSGEGVQQALLKIIEG
                     TISNVPPQGGRKHPNQEYLRIDTTNILFICGGAFVGLDKIIESRVSSHAMGFGADVKA
                     RHEKILSQLYSELQPDDLIKFGLIPEFVGRLPVKVALDELTKEDLKRIIVEPKNALLK
                     QYEASMRLDNVELEFDNAAIDAIAEKAIAQKTGARGLRAIVEKIMVDIMYSLPSDNNV
                     KKVILTRDAVEGKGSCIIEYRQKTA"
     regulatory      17370..17480
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            17467..18501
                     /locus_tag="PLS88_01565"
     CDS             17467..18501
                     /locus_tag="PLS88_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU06075.1"
                     /translation="MMRKKQFIAVFEVIAMVSIAVVTGWAQSNAQSKQQNSELIVWTY
                     DSFISEWGPAPLIARAFQEEAGIKLRFVSKGDGGALLAALFDPINKPSADIIIGLDDK
                     QLDKALKSGLFIPLSLKNISEIQKDLLLDTTNRLVPFDFSQFALIWDSQSGIEPPRSL
                     EDLTKPIYRKKLIIMDPRTSTPGLGFLAWTEAVYKTDWKEYWKRLAPSVLAMTPSWDT
                     GYGLFTKGEAPLVLSYSTSPAYHKAYENSERYRALQFAEGHPTQIEFAGVLISSQKKE
                     LAQKFLDYLLSAKCQALLPETQWMFPANIKTELPPSFSIVQKYPALIATIRDLDHDPS
                     TAANLLIGNP"
     gene            18498..20159
                     /locus_tag="PLS88_01570"
     CDS             18498..20159
                     /locus_tag="PLS88_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU06076.1"
                     /translation="MRTRIFLYLVLCTILIFSLAPLIAFLQAAFFGLSEAESKTEAIK
                     TFLDFFKRIPESTIAKSLWFTLKQSIASTALAIGLGLPGAWLTARFRFPGRKALLSLA
                     AIPFCFPPILIILAFISYYGKEGLLSTAFSLIFGSRGEYRGILYSFWGLVIVHAFYNF
                     PIVVHQISALLLRIPNTPLEAARTLGAKKLKAFRTGILPWIMPGIGQSAGLIFLYCFF
                     SFTTVLVFGGRAGTTLEVEIYQALRYRSNLALAAVFSVIESACALLGIWMLTRIKGKT
                     YRTVRDFGAARKLVKPKGKQWFLIVLYQIFIIVFFIGPLFSIVIEAFRVPASLNSPDK
                     FGFDNFKRLLTGFQAPLTGAILSTLKLSGIAALLATFIGIIACFSIFFAQRGEKKSNI
                     VAMMMDLLQWLPMAVSPAVIAYGWLFISANHLATSALIAAQALIAWPFVSRTVGASLR
                     AIDSRMHEAAKTLGASPIKAFITIELRTILPSVAAAAAFAFSITAGDVNVPLMLGMSE
                     IETLPLILYRLTAAYRFNEACAAGLVLGLMTGIVFMLKEKVIDVA"
     gene            20149..21243
                     /locus_tag="PLS88_01575"
     CDS             20149..21243
                     /locus_tag="PLS88_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU06077.1"
                     /translation="MSLEVKDLLVERNSFVLRADFSLEQGKILVLLGPSGCGKTTLLR
                     SIAGLEMIKQGTITLNNLRIDKLPTEKRHIGFVFQDLALFEQLRVRDNIGYSLSIRNE
                     EKRKREERVISLARNFKIEHLLGRYPYELSGGERQRVAFARAFAADPMLMLLDEPLSS
                     LDAPLRREMRRFIRVQLSRQQLTTIHVTHDVEEALDLGDEIIVMRDGKMISRGIMSDF
                     EKKPGSGWLARFMNLGLVLPIIEISASKESQLLDIMTPEENMFQVSRNLESNIDNALE
                     KAMCLYIPFSAIKIFRKNNNEKTINVKIVRYLTSTPLVSKLVLTLPSMQDYFFELPMP
                     ESDFPMPAEGTEIEISIDQSMCQVLPDIPL"
     gene            complement(21216..21758)
                     /locus_tag="PLS88_01580"
     CDS             complement(21216..21758)
                     /locus_tag="PLS88_01580"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOU06078.1"
                     /translation="MKKIALIGMMGSGKSEIGCLLARNYGIDFIDMDRSIEENTKTTI
                     PAIFRDFGEEYFRDLEENQLAIMAATPRSFVLSCGGGVVLRDFNRMILKNSFLTIWLD
                     VSIAELKRRLQDQRHQRPLLQSEEWESKIEEIYCQRHELYRSAARIRYAWENNHSLEE
                     SAIDIQKLIDAAHNGISGRT"
     gene            complement(21755..22756)
                     /locus_tag="PLS88_01585"
     CDS             complement(21755..22756)
                     /locus_tag="PLS88_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOU06079.1"
                     /translation="MLNNNNQDDFYVIDAHCDTLMAITGRSMNKEERNPRDFFADNTS
                     VHLDFPKLVRGRVVCQVMAIFLEDSQLQEATSETMAMIDSFESLLKFAPKEFFLAKDG
                     ADVHRALETKSVCAILSIEGAEALGDSLESLDIFYQRGVRAIGLTWNRRNAFGRGLKA
                     EGSDGLTSLGKELVEKMEEMHVLVDVAHLGEEGFWDVADISTGPFVASHANARGILDH
                     PRNLSDSQIRAIADHGGAIGCVFVPYFVTKDPKDCSLEAFLLHIDHIVRIGGIESCAI
                     GSDFDGFLPQEGKVIQDAGEFPLLYSALRRHGYSHEAAQKILGRNWLRIFDDVFGSI"
     gene            complement(22768..24750)
                     /gene="uvrB"
                     /locus_tag="PLS88_01590"
     CDS             complement(22768..24750)
                     /gene="uvrB"
                     /locus_tag="PLS88_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOU06080.1"
                     /translation="MKPFRVVAPFQPAGDQAQAIETISKAIKKGEKYVTLKGVTGSGK
                     TYTMAKVIEVIQKPTLVISHNKTLSAQLYREFKEFFPENAVEYFVSYYDYYQPEAYVP
                     SKDLYIEKDSDINEEIDRMRLSATASLMERRDVVIVATVSCIYGLGSPDLYREMRLYL
                     DRGQIIDIDEIKRNLISLQYERNDMVLERGRFRIRGDVLEIFPAYLEDAYRIELDFDT
                     IARIRRLDPLTGEASNELEEAIIYPAKHFVMPEDMVQRAIDSIRNELEERYRYFIEHG
                     KYLEAERIKSRTEYDIEMLSEMGYCSGIENYSAPLSNRKPGERPGVLLDYFPKDFICF
                     IDESHVTLPQLGAMYNGDHSRKLSLVDYGFRLPCALDNRPLRIDEFESIVSQTIYVSA
                     TPGEIELKRSKTLAEQVIRPTGLVDPEIIVHPTEGQMEDIYARVRERAKQGERSLIIT
                     LTKKMAEELTDYLSGLGLRVRYIHSEVETIERVEILTQLRAGEFDVLVGINLLREGID
                     LPEVSFIAILDADKIGFLRSETSLIQIIGRAARNAAGLVVMYADVISPAMKNAISETE
                     RRRKMQQAYNLEHGITPRTISKKIQDILERHKEERIEASQGEIEMLRHTHNLLVPEQR
                     KSLIKELEKLMFEHAKNLEFEQAAIIRDEISKLKDL"
     gene            complement(24747..26018)
                     /locus_tag="PLS88_01595"
     CDS             complement(24747..26018)
                     /locus_tag="PLS88_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOU06081.1"
                     /translation="MKLYSKAQICMVIAVSVALTALSIVGIVFVIDKHAQSQSTMIMA
                     SQPAKDTEEDVYFPEITSLGSQDDQTVGLPLQIANSAQSMSGYSADEIENISIYEKYN
                     ESVVNINTEVLGINWFLEPVPQSGGSGSGSIIDEQGYILTNNHVVEGAYKLYVSLADG
                     SRYEAKLIGTDPESDLAVIKFSPEPGKKLRPIPFGTSKDLKVGQKVLAIGNPFGLERT
                     LTAGIISGLGRPIQESETIILQNMIQTDASINPGNSGGPLFNMRGEMIGINTMIYSPS
                     GGSVGIGFAIPVDTAVRIVPQLIKNGQVRRGWIEMQVIQLFPDLVNYLRQTGKAAPVE
                     TGLLISTVTSGGNAEKAGLKGGTTAVRYRSTVFYIGGDIIVSIDGKPVTKIAELYAAL
                     EDTSPGQRVTVEFYRGSKKMSTTVVLAERKR"
     gene            complement(26096..26617)
                     /locus_tag="PLS88_01600"
     CDS             complement(26096..26617)
                     /locus_tag="PLS88_01600"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOU06082.1"
                     /translation="MSIITKTGDEGITGLWSGEKIGKDSPRVEAYGTIDELSSALGIA
                     RHLCIQDNVLCAIEYIQRLLFRVGGELASLSMPFDNPINEDDEKTIEDKTKELEMRIP
                     IRSFVVPGMTGGSAALDLARTIARRAERRVVALSRIEDVSPVLMRTLNRLSDYIYMLA
                     REEEAAQGKLTFV"
     gene            26723..27649
                     /locus_tag="PLS88_01605"
     CDS             26723..27649
                     /locus_tag="PLS88_01605"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06083.1"
                     /translation="MTTENKVILYFGAQGTCPYLFNNHQTLMNLLVFQDEVDPRLYSA
                     LISTGWRRHGKNIYRMECPSCELCIPLRLEPMSIQLTFSLNRILRKNKDIVIIPTPPI
                     FRAEHFMLWQRYSLWKHSIPLKDLAEDSYNNLLEPWSLIFEYRDNNEDRKLLAVSHVD
                     PLDDGLSSVYFSFDPDEKGRSLGFYSILAESYLSSFVKNAKYLLPNKEIKGESVSYFY
                     ENRISCKLEPGYTKNQIAAIGYENEHYYYLGFWVPGAPKMDYKARISPFEISIADENI
                     DNPNPWKRFFSRKEAITYLSRREWPGLSLTIP"
     gene            27690..29294
                     /locus_tag="PLS88_01610"
     CDS             27690..29294
                     /locus_tag="PLS88_01610"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOU06084.1"
                     /translation="MKGQLMASEENEKQAKTGNKKSSEKKSNNKQAKTTLKKTIKNPF
                     VYIGTIIILVLTIIAFVLIPSISSGTSASGKIPSFGSWNGNPIQYTSGSYFAEQVSQI
                     NDYLRQQGLDETQSQLYAYQVWRLAYQNTVIRTAILDSAKRSGMKISEETIDEEVSKN
                     SQFQVDGKFSLEKYNSTSAATRLSIRNRIREDMLIQNYYNDLMSASPSTAEIEFVASM
                     AKPQRAIQYVNISYADFPAEETLEWAKSHESLFRTIGLSKITISTSEKDAKKVLAQIK
                     ANSLSFEDAAKSHSKDIYADKGGDMGTRTFYDLKSEFLDEKDAENLMSLKKGEISPVY
                     KQTGNTWAFYQINSESSPPDFSKQSVLDEVKSYIFDKEKSVLESWALAKANEFKAETM
                     NTSFESAAKITGLQVKSAGPFILNYGNPGFYFYGQQISLFQEPYRSLDTDLVGAVENE
                     TFLTTLFSTPKDAVSEPVLLDASVVVMKVTEDSEASDQEASYTKFSYPYFFQTAMENH
                     IRNSILSSEKFKDNFNTTFAKLFMAN"
     gene            complement(29358..31172)
                     /gene="lepA"
                     /locus_tag="PLS88_01615"
     CDS             complement(29358..31172)
                     /gene="lepA"
                     /locus_tag="PLS88_01615"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOU06085.1"
                     /translation="MIEPACIRNFCIIAHIDHGKSTLADRFIEKAHLIDSRKLQDQML
                     DNMDIERERGITIKSQAISLPYIAKDGKTYMLNLVDTPGHVDFSYEVSRAIAACEGAV
                     LVIDATQGVEAQTLSNMYMALEHDLEIIPVINKIDMASADIEGVRGQINKDLGLEGES
                     ALAVSAKLGIGIDELCEAIVERIPPPNGVAENPLQALIFDSHYDPYRGAIVHLRLKEG
                     SIRKGMHIRLMSTGAIHEVEEVGHFRMALSPAEELSAGEVGYCIAGIKTVKEVRVGDT
                     LTDYENPCKTALPGFREVKPVVFSSIYPMDSSDYEELRDSLEKLVLNDASLIFEKDHS
                     LALGFGFRCGFLGLLHLEVVQERLEREYGQSIIMTAPSVRYRVIFKDGSTLYIDNPSD
                     YPDPSRIESVEEPYIKAEIITPVDYVGNIISLCVGKRGSQESFLHLDEKRVELVFEMP
                     LAEVLFDFYDKLKSTSRGYASFDYEVTGYRLTDVVKLDFLINGEPVDALSMLVYRPNA
                     YDRARIVCERLRRNIPRQQFKIPIQGSIGSQIIARETVNPVRKDVLAKCYGGDITRKR
                     KLLEKQKEGKKKLKTIGNVELGKEVFLSVLRASEEEDR"
     gene            31290..31592
                     /locus_tag="PLS88_01620"
     CDS             31290..31592
                     /locus_tag="PLS88_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOU06086.1"
                     /translation="MNLENLSIIEKGGLRKDHEIMVYALSTCIFCKKALAFLDANNFA
                     YKYLFIDRIPFESKNAIKKELGERFKENIIFPFMVIDARKHLIGFIESEWKMALGA"
     gene            31600..32781
                     /locus_tag="PLS88_01625"
     CDS             31600..32781
                     /locus_tag="PLS88_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOU06087.1"
                     /translation="MKDIYLDWAATTPLDSDIMRSALEFSLRAYGNPSSKHWLGKEAR
                     QALEESRARVMHSIGITKGNFVFTSSGTEADHIPIFAILERLRRASSAGKPIHAIISS
                     IEHSAIEEQIRVLERLGLQVSRIDPDSKGHLEAEHVASKIRNETVIICIMNVNNETGA
                     IQDIKAISESIREVVQLKRMKKPWLHVDAVQALGKLSIRDLENYADSFAVSAHKIRGP
                     KGTGGLWFARPPEIFIRGGGQESGLKPGTENLFGAFAFSLAAEKAALSMPANQEKALI
                     LEERIIDGISSIPNALIIPERIPKDNGFVPSIISMAFPGLGGETMVRALSDCHIAVST
                     GSACMSNIHHAERRVLKAMQVPEDIAFSSIRVSTGPLTTLQDIDSFLEQVEVLYRKLK
                     T"
     gene            32795..33985
                     /gene="thiI"
                     /locus_tag="PLS88_01630"
     CDS             32795..33985
                     /gene="thiI"
                     /locus_tag="PLS88_01630"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOU06088.1"
                     /translation="MHTLFLIKPGEIELKLGNKREFARRLKEQITKRLKGIPFTLEEY
                     PGRFFLTVEEKDSELTLFVLQHCPGINGVAKAIKTKKDIDEICLAAVECAKTEILTGA
                     HTFKIEARRSDKSFPFDSYRICVIAGNSVLAEYPTLSVDVHNPDFIIRIEIRERAYIY
                     SSTAAGPRGLPVGSQGKGILLLSGGIDSPVAGYMMACRGLALESVYFHSYPYTSPEAQ
                     QKVERLAARIAIWTGGMHLWIVPFTDIQLAISKTSYEETKTLMLRMAMMQAADIIAHR
                     INANSIVTGESLGQVASQTAENMRLSQSKTSLPVLRPLIGIDKEEIIAIARQIETYEI
                     SILPYEDCCVVFSPKHPILKPDYEELKGYFESLALNNLIESAVMKSERKYFDFAYALK
                     TNTK"
     gene            complement(34018..34089)
                     /locus_tag="PLS88_01635"
     tRNA            complement(34018..34089)
                     /locus_tag="PLS88_01635"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34054..34056),aa:Glu,seq:ttc)
     gene            complement(34230..35528)
                     /locus_tag="PLS88_01640"
     CDS             complement(34230..35528)
                     /locus_tag="PLS88_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU06089.1"
                     /translation="MNDLENIPNKTLLEKLYNQHRAGYEEALANTVHDISKLLQDKGI
                     HPTIKSRVKDFESYFSKRIRLLKKAWDEHGNPLPINDVLAMRIICPFLHDLEEVVSLL
                     GQRYEVVEIEHKGQDRSFREFGYESIHVLIRIPDELVPLCEGLERYVIEIQLRTILQE
                     AWAEVEHEIVYKAEFTPLDEPLKRKLAALNATLTLSDIIFQEILDYQKKLSDALKKRR
                     EGFYGKIEQKTAEIFGISEATTEILSPFSAQTIEQGETATFHENNISAPIRWNGLDMD
                     MLLLSALNAHNASDFKSAISIYSKILDKKPEKSLAAVVYKHRGMAYFAQSEYEEALAD
                     FTSCLMLDPNCYKALYYRGVVKSIQKELTQAIEDFSKALEIHPYHFFSRYRRALCWWQ
                     LGDYVQAHADCELALRIMPENKLAQELQKKIMEHLAKEDI"
     gene            complement(35698..36150)
                     /locus_tag="PLS88_01645"
     CDS             complement(35698..36150)
                     /locus_tag="PLS88_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06090.1"
                     /translation="MNPSKEQDHKQGYIVALIIFLTILFIVWKGPTLQYNCMRLYFPD
                     KKKGELKLEKRPIAPIGTLEEKAQATIEELLLGPLSRDLKPLTYLDIKLLRIVEGKNA
                     LFLDFSTQDVARLSSEYKTFKSALEKSLKETIPGTFHIYIFINGILAR"
     gene            complement(36152..37231)
                     /locus_tag="PLS88_01650"
     CDS             complement(36152..37231)
                     /locus_tag="PLS88_01650"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOU06091.1"
                     /translation="MYKLPDMMLVLISRKKFCLKILPGTIAIIAMLVLSNLALFAANE
                     KKQEDISISDLAYSLKATLEYDPLLHAGTITKEGRSVRFAVDVPYVLLDWTIVKEVPA
                     PYESVTSLQIKNEFTQTVQEFFELKPQTSSKYVVKAIVIDPGHGGKDPGAIGEFEDFR
                     LQEKDVNLSIALRLADLLRLRYPERTITLTRSSDIYPTLEERVEMANKIKLEETEAII
                     YISIHANASFNKNTKGFEVWYLNPNYRRTVVDEKTAKEKGEDIAPIINIMLEEEFTTE
                     SIILAQKVYQRMDRIIGDRSPARGIRAEEWFVVRNAKMPSILIEVGFVTNKSEALLLS
                     QAGYLRQIADAIYNGVCDFIEHFEQ"
     gene            37457..40963
                     /locus_tag="PLS88_01655"
     CDS             37457..40963
                     /locus_tag="PLS88_01655"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOU06092.1"
                     /translation="MKIERLFTKAGYGPYEKIKWEKRKSEIRNPDGSIVFSMDSVIVP
                     SFWSSISSDILAQKYYRKAGVPKDKAIEWKKFIPASQTALSNEDIVDDAEHDARQVFH
                     RLAYTWCDWGQKDGYFDSEEDRQAFYDEICYMLAHQIAAPNSPQWFNTGLFSVYGIEG
                     PAQGHYFIDPNTKEVAKSESAYKRPQPHACFILDVKDDLVNEGGIMDLVTKEARLFKY
                     GSGTGSNFSRLRAANEKLSGGGVSSGLLSFLKIGDRSASAIKSGGTTRRAAKMVILDV
                     DHPDIEDFVRWKTEEEYKVACLAAGSGLLNHYAQVIISAIEDMGAQDENSYLPDRNLL
                     LRKAIKQALNAGLPSSWIHQVLTYYAQTGKAPRMPQYNTAWESEAYNTVSGQSSNNSV
                     RVTNEFMEAVINDLDWELIARTTQKPFRVTKARNLWNEIAESAWFCADPGLQFHTTIN
                     EWHTCVADGEIRASNPCSEYMFLDDTACNLASLNLLSFYDFKAGKFDEMAYRHAIRLW
                     TLVLEISVAMAQFPSKEIAKNSFDYRTLGLGYANVGSLLMVMGLPYDSENGRTVTAAL
                     SAILTGEAYAQSARIAREFGPFARYKANKESMLRVIRNHKRAAYNAAKEEYEALSVFP
                     KGLDSQSCPSNLLNAAHECWDEAIQLGEKYGFRNAQVTAIAPTGTIGLIMDCDTTGIE
                     PDFALVKFKKLSGGGYFKIINASVPPSLHALGYSEDEIADIIQYALGKGTLEGAPGIS
                     LQSLVDKGLPNNSIDRIEKALKNAINLDSCFSLYNVGYDVLEKLGISKEEANTPGFSL
                     LSRLGYSDREKEEAEVYACGTMGLEGAPHLKSEHLAVFDTATPSGKRGSRSISWQAHI
                     AMMAAVQPFITGAISKTINMPNNVSIDDIKGAYMLAWRSMLKSIAIYRDGSKLSQPLA
                     ALTPGSNIIADSIIALQSGETTLEDVASLEGTSDSSTKKNVEPELPGLPVAPRGIRRA
                     LPNRRKGYTQKAKIGGHSIFLRTGEYEDGSLGEIFLDMHKEGAAFRSILNSFAIAVSL
                     GLQYGVPLEEYIDAFTFTKFEPNGIVSGHDYLKMATSVLDFIFRDLAISYLHRYDLGQ
                     IKPEDLIATSTQSLSNKTSSAFESGKRHIPTERDTNIPLIREARQKGYEGDACPVCGN
                     LTLVRNGTCLKCETCGSTTGCS"
     gene            41239..42564
                     /locus_tag="PLS88_01660"
     CDS             41239..42564
                     /locus_tag="PLS88_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOU06093.1"
                     /translation="MKKRKIIIIGAAGRDFHNFNTRYREDESTDVVAFTAAQIPNIDG
                     RRYPAALAGRLYPEGIPIYSESELPDLIKKYDVDECVFSYSDVPYSHVMKMSAIVNSA
                     GASFTLLGPKDTQLKSSKPVISVCAVRTGSGKSQTSRKIVQMLMKRGLKVVAIRHPMP
                     YGDLVQQKVQRFATIEDLARYHCTIEEMEEYEPHIARGNVIYAGVDYEAILRQAEKEA
                     DVILWDGGNNDFSFYIPDLQITVADPLRAGNEVSFYPGEVCLRTAHVVIINKIDSASP
                     EQVQIVRDNIARTNPSAIVIDGASPITVDRPELIRGKRVLCVEDGPTLTHGMMKFGAG
                     VVAARKFQASQLIDPRPYLSGGLKQTFNTYPDIGTLLPAMGYSETQKRDLEDTINRTD
                     CETVIIATPIDLNRIIHINKPTVRVDYELQEIGSPNLDEVLEDFCKRKL"
     gene            complement(42666..42836)
                     /locus_tag="PLS88_01665"
     CDS             complement(42666..42836)
                     /locus_tag="PLS88_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU06094.1"
                     /translation="MAYRVTEACTNCGSCESECPVSAISEKDNARYIDPETCIDCGAC
                     AAACPTEAIIAG"
     gene            complement(42944..43756)
                     /locus_tag="PLS88_01670"
     CDS             complement(42944..43756)
                     /locus_tag="PLS88_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOU06095.1"
                     /translation="MMPFAEEKEPFIIGQDDRVCAVYKPCGFHSVSQKKSDHSIVSWL
                     YDQRIPGLKGSLVGRELGLVYRLDQATSGILLFAANRDSFIRLRQAQNELAIAKRYIS
                     ISAPSECELPGSLPKTMKERQSFFIEELLSNKEVYIESFFRPYGPKGALVSCIAPEFA
                     SKSNKPITKEIYISKYIAAYKVMEVSNVKRQLPNGVVCLEVEIHKGFRHQIRAHSAWI
                     GLPILGDMLYGEQDSNRLWLEAFLVCLPQQDGCRNEWKLYDGIKDIAQYLSI"
     gene            complement(43757..45856)
                     /locus_tag="PLS88_01675"
     CDS             complement(43757..45856)
                     /locus_tag="PLS88_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06096.1"
                     /translation="MDEYFDAEPVTKSYDAQIARRILLYLKPYRLYAIIAILALAIAT
                     AGELISPTIIKRAIDDVVVRDWYGIDPSAKYILPKDEGSLEVRIGGRIYVRASRLAGL
                     TMAERNRLFEQGLLDNEASYVFPLDPMREEKISIAERISKVELSDNWGVIPSSVLRTL
                     RSEDAAILRAADDKALLRFGLILLIVLLIVLGATFAMTWFTNQISTLIMKDMRLELFH
                     HVIEQSLSYLSRQPVGRLVTRLTSDIEVISQFFSDVLSAFIKDASIMIGSLIVLFLLS
                     WKLAFVVLATMPLVFIASAVARIKARDAFRNQRYWTSKVNSFLSEHISGIDIVKLFVQ
                     ENKVSEGFGKNNKQLLKANIAEMYVYATFRPFVDFMATLTTVLAIFFGAMLFLRMQIS
                     IGTLIAFISLISMFYSPIKDLSEKYILLQSAMASGERIFGLLDQDERLPDQLPEFVGK
                     PFPRTIRGHIEFSHVWFAYKNEEWVLKDVSFIVNPGEKVAIVGYTGAGKSTVANLLAR
                     FWDIQEGSILIDGVPIGQFPLKQIRRLIQPVPQDVFLFQGTIRENISLGLDLSQEKLE
                     EAAKAVYAHDFIISLPQGYDTPLAEGGLNLSLGQRQLISFARVLAHEPSVIILDEATS
                     SIDTETEKLLQRGIEGLLKGHTSIVIAHRLSTIRNSNRIIVLGQGRVVEIGTHDELIA
                     LKGLYWNLYRLQNKEAE"
     gene            complement(45849..47690)
                     /locus_tag="PLS88_01680"
     CDS             complement(45849..47690)
                     /locus_tag="PLS88_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06097.1"
                     /translation="MFKEYSTLLPHLRSYRYRYVGGILCLIIVDASQVLIPRYIQMAI
                     DRITSGTFVFTDIISPLFHMLSLALLISIGRFFWRYFINIASRRIEAEMRDRLFSHIL
                     SMSGNFFRKNTTGDLMARATNDISTIRQATGMGFVSLIDGIFMTGMIIAAMFANNSAV
                     AAWIILPLPLITALILLFGRIVGKLFKKIQDIYGHLSDIAQESLSGIRIVKSFVKEEY
                     FFKKFSIANTEYKNAIMDLVKTSGFFFPFITFLSGLSTVLLILFGGRASIRNKMTPGS
                     IIAMLSYLEMLVWPLMSAGFTVNIIQRGAASLKRINEILRTEPEIKESSNCLKQMPRG
                     NIDIKKLNYIYPGTQKPALKDISVYIHEGSMLGILGKVGSGKSTLLKILPRMLEPGEG
                     HVFIGNIDICFYGLSELRSAFGMVPQESFLFSDSIRQNILFSADNISPERFEAITRIS
                     GLDHDYQLFPNGWETIVGERGITLSGGQKQRIALARALAADPYILLLDDALSAVDAET
                     EEHILSSLIDERKGKTTIIVSHRISTLRNADHIIVLDSGEIIQQGSHEELIADSEGFY
                     ARIAALQQLEQQASIPSIGDSISQRETQVEQKSLAHDASVSEETIYG"
     gene            47767..48309
                     /gene="rnhA"
                     /locus_tag="PLS88_01685"
     CDS             47767..48309
                     /gene="rnhA"
                     /locus_tag="PLS88_01685"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOU06098.1"
                     /translation="MSQEDFIVYTDGGCKGNPGPGGWAYIMRYGDRYREAWGGDPYTT
                     NNRMELTAVIKALSFLKSRIEDASSSASNSSLESPPSWTKAQINIFTDSMYVKNGITK
                     WLAEWKKRSWKTSAKKPVMNLELWQELDSLCCKLEPHFEWVAGHAGNIDNERCDKLVH
                     LAIEDMLRQKESGTSIVVPL"
BASE COUNT        14030 a        10529 c         9940 g        13886 t
ORIGIN      
        1 caagtctttc ttttatctta ccttagaaaa atcatcacaa atttgtcgtg cacccttttc
       61 aataccaaat ctagacaagg aacataattg tggtaaaata aatacataaa tatgctttat
      121 gcatatttgc gacggctctt tattatgtcg aaccgccatt ttcgaagaaa aggagaagaa
      181 tatgccatcg acaggaaaac caatgaccaa ggtcgagatt attgatgcat tagcaaagtc
      241 gacaggcttt actaagaagg acatcaatct tgtgctagat gaacttgcca aactcgctta
      301 ttcagaagca aagaagaata aaaagtttac tatcccaggc atagcaatta tgaaagttgt
      361 ccatcgcaaa gccaaggttg gacgtaatcc agcaacagga gaacagatca caattcccgc
      421 gcggaatgtg atcaagatta ctgtagcaaa accctgtaag gatgccgttc taggtaaatg
      481 aatcgctttt aagaattcta ggaagtatta aaacgcatgg aatgtccccg aaataccgcg
      541 ctatcacggg gacatttttt gatctttgga tttttcttcc ctatttcagt cagattgagg
      601 cggtattata gctctttatc ttttgctgta tgtacgatgt tagattagca cgggatactc
      661 tttcttgaag catgctatcc ctatctcgaa ctgttacact gttgtcttct tttgtttggt
      721 aatcgatagt tatgcagaaa ggtgtgccaa tttcatcctg ccgtcgataa cgtcgaccaa
      781 tggcgcctgc ttgatcataa tctgttacaa aatcatcttt taattctatc cgaatattct
      841 gagcaagctc agctagacca tcttttttca taaggggcag aaccgcgacc gtaataggag
      901 ctaaaattgg atgaaaatgc agcactgtgc gccaatcatc ttcatttcct ttatcggata
      961 tcttctgttc ttcgtaagaa tcgcagagca acatgagaag ctgccgagta agaccagcgc
     1021 tcgtctctat gatgtatgga atatagcgtt catttccgct gtcttggtcg atatattgca
     1081 gatcttttcc cgaatattgc tgatggcgac tcaaatcgta atcagtgcga ttatgcactc
     1141 cttcgagttc tttgaagccc attgggaact catattcgat atcataggca tctttggcgt
     1201 agtgagctaa ctcatcggct ccatggcgat gccatcgcaa tttcttcata tttacgccca
     1261 ttttcttgta atagctccag cgctctgcac gccaatattc gaaccaatct tcgtctgtac
     1321 ctggcttaac aaagaattgc atttccatct gttcaaattc acaagtcctg aatataaaat
     1381 tctttgtgac tatctcattg cgaaaagctt ttccaatctg tgcaatgccg aatggaatct
     1441 tcatgcgatt ggactgctga acattcttaa agtttacata tataccttgc gccgtttcag
     1501 gtcgcaggta gacaaggcca gattcatctt ccactgggcc taaattagtc ttgaacatga
     1561 gattgaattt gcgagtatcg gttagctcgc cgccacagtc tgggcatttt ttttcagcaa
     1621 gattctcggg aggaatctga tccgccctga agcgtccttt gcattttttg cagtctacaa
     1681 gaggatctgt gaagttttcg acatgtccag aagcttccca taccctgggg tgcataagaa
     1741 tggctgcatc aagacccact atattatcat gcatctgggt catttctttc caccagaatc
     1801 gagcgattct attttttagc tcgattcctt gaggaccata atcccaggct cccgaaaggc
     1861 cgccatatat ctctgaggat tgaaatacaa aacctcgacg ttttgcgagg gaaacgatct
     1921 tttccatagt tactgtagtc tctgacataa atcttgtctc ctttgctgga atatcatggc
     1981 ttaattattg aggaccatgg aattaatgag gcttttcctg aaatttcttt tcgatttttg
     2041 cccaagtatc cctcaaagtt acagtacgat taaaaacggc caaactgctt gttgactcgg
     2101 ggtccttgca aaaataccct attctttcga attgtactgt ttgacctttt tcaatttcga
     2161 ctaccgctgg ctcggctact gctcgggatt cgattgtaag tgagtgatca gagagattgt
     2221 caaaaaaggt cttaccttca ggcacttcca ttgggcgttc cgcttcgaag agatgatcgt
     2281 atagcctagc ctcaataggg attgcgtgga tagcgcaaac ccattgaata gtgcctttta
     2341 cttttctatt gtcgggtgca tctccacccc ttgtattggg gtcatagacg catcttatct
     2401 tctcgatttc tccactggtt gggttttttt caaaaccagt acaagtaaca atatagccat
     2461 agcgcaatcg aacctgattg ccaggataaa gtctataata tttgggcgga ggcacttctc
     2521 taaaatcatc tcgatctatg tagagagtac tactaaaagg aatctttctt attcctgcag
     2581 aaggatcctc tggattattg acagcttcta gccattcgat tttcccttct ggccagtttt
     2641 ctatgacaag cttcacggga tttataacag ccatataacg atttgcatgt gtatttaatt
     2701 cttcgcgtat acaatgctct aatagctgaa tatccacgag ggaatctgtc tttgcaatcc
     2761 ctattcttcc tagaaaatcc ctgattcccg ctgcaggata tccacgcctg cgcattccag
     2821 caatagtggg catgcgcgga tcgtcccaac ctttgacctt cttttctctg acaagttgaa
     2881 gcaaccagcg cttggagagt acggtatgag agaggttaag acgagcaaac tcaatttgac
     2941 gactaggaaa tacttcgcag ttttgaataa accactcata caagggtcta tgaatctcgt
     3001 actctaggga gcatagagaa tgggttattc cttcaatggc gtcggagaga gggtgagcaa
     3061 aatcgtacat tggataaatg caccattttg ttcctgtcct ataatgagga attcttctaa
     3121 ttctgtacat tacagggtcg cgcatgagga gattaggatg agccatatcg atctttgcgc
     3181 gcagagtctt tgaaccatca gcaaattccc caacgcgcat cctttcgaat agatcaagat
     3241 tctcctcagg agaacgatct ctccatggac tatttcgacc aggaggagta taggtaatat
     3301 tattcttatc cggcacattt gtgcctcgaa attctttgat ttgttcttca tctagatcgt
     3361 cgacataagc aaggcctttt ttgataagtt tacaggctaa ctcatatagt ttctcatagt
     3421 aatcagaagc aaaaaaaagc ctatcttccc agtcaaagcc tagccatttg atatctcttt
     3481 tgattgcttc tacatactcc atatcttctt tctctggatt tgtgtcgtcc attcgcagat
     3541 tgcatttgcc accaaagtcc tgagctacgc caaaatctac gaaaagtgct ttagcatggc
     3601 ctatatgaag ccatccatta ggttctggcg ggaatctagt acggacttct ttgtatcttc
     3661 cggtgcgcaa atcctcagca accgcctcac ggataaaatc aaggtattcg ggtttttcga
     3721 ctaattcaga catgatataa ctctccttgc tgcgcttcag aggcacagta agcgaaatat
     3781 cgcttggatt gaatggctag gtcaagctcc tcgcagatag gctactgcag cctcgattct
     3841 tttaatgcat tcatccttgc caaggatccg catcgattcg aatagaggcg ggctgacacg
     3901 ggaatatgtt atagctaccc ttaaaggcat cagaatgtct cctagtttct ttcctatgac
     3961 tgtagcgcgt tctctaaatt tttcctcaat agcaggaaga tcgtcgagat tcgagctctc
     4021 gaggatcgat ctacattctt caagatacat tattgcttcg tcgatgctag cttttttggg
     4081 aacaaattct tctgctgcag gcatatctag acgtttataa aggtaagaca tgatcgcagg
     4141 cgagtctcca agcagcttaa gtctttcttt gatgaatgga atcgcagcca gctccatttg
     4201 atctttttga ggatcttctt cttttcgcag gccggcactg atcaaatacg gacggataag
     4261 ctctgccaac tctttatcgc ttttcatacg aatatattgg ccattgaacc attccaattt
     4321 ttggtagtcg aataccgctg gcgccttgtt gagccggtct attctaaata ggcggacaag
     4381 ctcatctaaa ctatatatat ctcgaccatc ttcgtatgaa catccgagca aagcgatata
     4441 attgatgagc gcttcaggaa gatagcctgc ttttttaaat tcattcactg ccgtggctcc
     4501 atgccgcttc gaaagcttat gaccgtccgg acccagaacc ataggaagat gacagagctc
     4561 tggcatctcc caaccaagag catcatacat gatcttgtgc ataggagccg aaggaatcca
     4621 ttcctgagca cgcattatat gtgtaattcc cattagatga tcatctatca catttgccaa
     4681 atgataggtt gggaaaccgt cagactttaa gagaactggg tccgggctta tatcttcatt
     4741 tttccattct atctggccaa gaagagcatc atgaaaaaca gtaattccat gaagcggaat
     4801 ctttagtctg attacatagg gtcttgcttc tgcgaggttg cgtgctctct cttcttctgc
     4861 gatatagcgg caatggcgat catagcctat ctcatctttt ttctgagcga cctgctcttg
     4921 gcgaagcttc tcgaggcgct cagaatcgca gaaacaataa taggccttgt ccataaagac
     4981 aagctcttct gcataccttc tatatagatc gattctttca gattgcacat aaggtccctt
     5041 agaaccaccg acatcaggac cttcgtccca ataaaaccca agccacctga atgtttcgta
     5101 gagattttgc acatattcct gtgaataacg ggttcgatct gtatcctcca ggcgcaatat
     5161 aaaggttccg ccctgagatt ttgcaaatag atagttgatc aacgctgttc ttacaccgcc
     5221 gatatgttga tttcctgtag gggatggcgc atagcgcact ctaacggaca ttattcctcc
     5281 tgatcaatct ctgtttataa ttctttaggc tttcctagat ttgaattact atacatttcc
     5341 atgtctttct atgcaagaat tgaaaacaat cagctttggt gctagcagaa cctcgttagc
     5401 aattaggatt caatagatcc ggatgctttt tgcaaaaata ttcagcatta aggccgcaat
     5461 ggcaaaaaca agaagaaaga actgaaaaat gaaacgccca atagagaaac gatccttccc
     5521 cgctatcttt agagctatca tgcttgtcat attaaagaga ctcacagcaa ggagaaagat
     5581 attgcatttt tcatatattg atctccatat aacgttgtat tcattagatc tgtcaaagaa
     5641 caacataacg aaactgctaa ctaagaagat aataataata gtcactgcaa aattcagaag
     5701 cttcttaaga gtatgtataa aagcagattg cgattctgaa ccgcttttgt tttccataat
     5761 gctaatatat catacttcaa tatggaatat ctctttaact ttaaaaaaat tggctatcta
     5821 caaggcaaaa gaccaaaggg ctgtatacta tgtcttgtcc gcgacgattc caaggaagta
     5881 gaaaaactcg tggtacatga gactgagcat ttcattgtat ctataaatct ctacccctat
     5941 aatcctgggc atttgattgt attccctaaa aggcatattg tggatattcg ggaatactcc
     6001 gatgaagaag agaaagaatt agataagcta actaaaacat gcctagaagc acttgataaa
     6061 tatgcaagcc cctcagccta taacattggc tataatatgg gcttgtcagc tggagctagc
     6121 atagagcatc tacacatgca cattatacct cgttatccga gagaaatagg tattgtagag
     6181 ctcatagctg gatcccgtgt actagtacaa gatccaaagg ataccctcca acaattaaaa
     6241 gagatattca gtaatctagc aatcctgtga tatagaaatc ttaagatcaa gaggcaaaat
     6301 cacaagaata ggcttttctc cagaatagat ctctatctct tcggagatcg tcttattgct
     6361 taagccaaat tcgctgcagt cccatttcag gccatccaat ggccatttaa gcccattcga
     6421 tcgcatgccc aaggctcctt cagagagagg aaaaacagag acgacactat ttattggcat
     6481 agaagcctgc aacttaaagc cgcgatcgac gagaaaagca ctctcgtgtg cagtatgcca
     6541 ctctttaata gggtccttgc gctcaaaaag agctcttata gcgagcagat gatcaagacg
     6601 ccctgctccg cctcccgcaa ggattatatc ttttgcacca tagcgatgcg ctgcatcaat
     6661 tgcaagctcg gtatcagaat aatccttttc ttctggaaag cgaaaaatct tttctgttga
     6721 aagctgggat aatattgcag gatctacact atcgaaatct cctatagcaa tatgaggctc
     6781 tataccggcc tttatacata tctcaagtcc ggcatctgct gctataacaa tatcgctatt
     6841 ctcggaaaga tgccgaatca cagatgaagg aggattatcg ccaccagtca caataagggc
     6901 tttcatatta gcttcctcat agacatagag ctgaagccta agatacactc caaaggcaga
     6961 tatgataatc cccagtatcg atccagaggt ccgtagaatc gcagacagaa tcatccaaag
     7021 tgggaaaaag gcattcctgg ttggtggtgc agtccgcgat tcatttctca aaagacctgt
     7081 gatagattac gatatcgcga ctgatgctac tcccgaagaa tgtatgatgt tattcccttt
     7141 tgccatacct actgggataa aacatggtac tataactatt atttctcgtt cacgtagata
     7201 taaaatagaa ataacaacgc ttcgaaaaga ggctgattat tctgatggta gacatccaga
     7261 cagcgtcacc tttgtcgacg atataaccca ggatctctcg agaagggatt ttactatcaa
     7321 tgcaatggcc atcgatttag ttcgcaatat attcatcgat ccattcgatg ggaaaaagga
     7381 cctaaaaaat aagattatcc gtgcagtcgg aaatcctata gagcgattcc aagaggatgg
     7441 ccttaggaca atacgcgcaa tacgtttttc cgcccagctg gaatttgcca ttgaaccaaa
     7501 tacccttgct gcgattactt cttgctccag caacctttcg agagtttctg cggagaggat
     7561 tcgcgacgaa ttctccaaga taatattgac agccaagcct tcgattggtt tacgaattct
     7621 atcggaaacc gggctaatag aagaaataat tccggaatta aaagactgca aaggaatacc
     7681 tcagccgaat cattgcgaga aggatgtcta cgaacacctg ctttcgaccc tggatgcagt
     7741 cattccggat aatctagaag aagaacggct acttactcta cgtctcgcgg ccctcttgca
     7801 tgatatcggc aaaccagttt gctatcgtac tgaaaatgaa actgtaagct tttatcgaca
     7861 tgagatagag tcagaaagaa ttgccagaca ggctcttatg cgactcaagt atccaaatcg
     7921 tgttatcgac aaggtttgcc accttgtacg ttaccatatg ttcgactacg aaccaaattg
     7981 gacagatgca gccatccgaa gattcatcgc tcgtgttgga ttttccacga ttcctgatct
     8041 atttgccctt cgattggctg atacgactgc aaccacagga aaacccatat cctggccagc
     8101 tctgtcagaa ttcaaagcaa gaattgatcg aattctagaa gaaaaacaag ctctttcact
     8161 caaagatctg gcaattaatg gagaaaatct tgctagaatt gggattcctc gcggcaaaca
     8221 aatgggcgaa gttctatctg agctcttgga tactgtgctc gaagacccag ctttgaatac
     8281 gaaagaaaag cttctagaga tagcaaaagc taagtatttt ggaagatttg attagatact
     8341 aggcctttat atatccttgt cagcctcaga acttcctcca gccaccaaat ctagaagtct
     8401 agcagtctcg gcatttctat tatgcagcac ttcgaatata tgctgggcaa ctcgctgctc
     8461 ggcttctttt tttgacattc cttccagagg gccaaaggtt tcgccatcga taacacagga
     8521 gacaaaaaaa tgccgagcat gttctggccc ttcgattttt atgaccagat atgaagggat
     8581 ttcaatacca agcttttgcg catattcttg aattatggac ttgaagtcct tgcttggtgt
     8641 ttttcgaagc tcttcgagct tactcttaaa tagccttgat atgaattcct tagttggtat
     8701 tatccccatg tccaggtagt aagcgccaag aagtgcttcg gtagcatcgg ctagaatagc
     8761 tggcttcatt cgtcctccgc tcatctcctc tccctttcca agaacaagaa attccccaat
     8821 attgagagac attgcgattg atgctagaga tgtttcggat accaagacat tcttcatatg
     8881 ggccaattcg ccttcatttt tatcaggaag attttcatat agaatgtgag atatgcatag
     8941 actaagaaca ctgtccccta aaaattctaa gcgctcattg tcttgtctat ttgaagaatt
     9001 ctcgtttaca gaagagctat gtgtaagagc tatatcaagg agtgtaatat cgtgaaaaga
     9061 aatgcctgtg gcttcttgaa agaacagcaa agcgctgtag cgtgaaggag agatctcttg
     9121 tccggagctt agccatgagt gatctttttg cttcacgcct tcgctcgtct ttttatttta
     9181 cctgggtctt gataaattca aaggcatctt ttacggtttc aaattcattc gccttctcgt
     9241 ctggaatctg aatactcaat tcttcttcga tgccataaac gagctcatag gtgtctagag
     9301 aatcagcacc aagatcttgg cggaaacttg catccattgt gatcttgctt tcatctactt
     9361 cgagcttgtc agcaataatc ttcttgattt tctcaaatag ctcttgctca tccattggaa
     9421 gactccttat ttagattcag gggtaattac ttgccgaccc ctgtaaaaac cgcattttgg
     9481 acaaacccta tgcaacataa ctgggtttcc acaattactg caatgtacga cagctggtgg
     9541 gttgagtttc atgttgatgg aacggcgtct tctcgtcctt gctttagacg tcctaaattt
     9601 cggtacagcc atggtagaac ctcataatta ttcaatatcg gcatgataat tgcatgccaa
     9661 attatacaaa atttataaaa atgtcaagat agacatgctc gcagtcgtta aacatatatt
     9721 atttgcgcaa gttatgaaag gggttgccct cttctctgta atatctgcct aagaaccatt
     9781 tcaacttctt ctggtacgat atcattagga agcctactat gcgatacaag atcacgcaca
     9841 agtgtagagc ttatatctac atgttcagca tcatagagga aaaaaactgt ctttatggaa
     9901 ggatcgagtc tttggttcat gtaggccata gtggactcat aaggcaattc agcagcattg
     9961 cggagtccgc gcacgagcac tgtgcatcca tgttgactag caaaatcagt cactagacca
    10021 tcccatttac ataccttcat tcgttgaccg aatctgttca tggcattgag catttcgata
    10081 cgctcatcaa tagtgaacaa tccatccttt tcagggttga tggcaacaac aacataaaga
    10141 ccgtcgaata tgctaagggc actcttgatt acatcgaggt ggccagaggt tggaggatca
    10201 aagcttcctg caaagaccgc atttggcata aattttaatt taatgcagtt ttacttcaac
    10261 agtcaatgga aaaaatctgc tctagctctt tagtcaaaaa gatctttcgc ttatactgat
    10321 tttattatga atgaattagc caaggggctg catcgacata atatcttaaa gcccgaaaat
    10381 ctcagttctt tttcaacacc tttctggatt cgcctgctat gggcattttt agctgcttta
    10441 tttgctgcgc ttgctcagcc aaatgaactt ttcttgtatg ggaattggtt tttcggcctc
    10501 ttctgcctcg tgccacttta tatggcactt gttgatactg aaaaactaga agaagcaagc
    10561 cttattggcg ctctttttgg agcactttat catgcgctga caagctattg gctttttttc
    10621 tataagaact ttgcattttg gactctaggg actacaacca tcgcttatgc agtaatatat
    10681 ggagtagccc ttatgtatgg atgttttttt ttgcaccata cggatggctg tagacctctt
    10741 gcattcgccc tagcatgggc agctctagag tttgcaaaat ctactggctt tctagggtat
    10801 ccttgggggc ttctggctta ctcctttact gggcttccga ttatgctgca gacagctgac
    10861 ctctggggcg tctatggtat ttctgctttc ttagccttat gttcatcagc aacctcagaa
    10921 cttattttgc aaaaaacacg caaaatgagc cccttaaaaa gttgttttcg ttggcctaca
    10981 atagccggta cctgggctat aatactactt ctttatggtg ctttaacaat ttctaggcct
    11041 attcccatca gggcaacatt gcgccttctg ctctgccaac agaacacaga tccatggata
    11101 tcgggagaga acgaagctct atacgctaat ataaaagtag ctaaagaggc tttgaaatca
    11161 atccaagaag aaaaaggcgc aatgcctgac ctcatagtat tcagcgaaac aagcctaagg
    11221 cgtccttata aggatttcca atcttggttt gaacataatc ctccagaaga acctctattg
    11281 ccttttctgc ggaatgctaa tattccattg ctgactggcc tcccagtggt ctggaattgg
    11341 gataccaatg aagcatccaa ttcagttggt attatttatc ctacaggtaa aatcggcgaa
    11401 acatacgcaa aaatacatcc cgtaccgttt gcagaagcca ttcccttctg ggaattttct
    11461 tggtttcgct cttttattca aaatgtaata ggacttgaaa gcggttgggt aatgggaaat
    11521 gaagaagtaa tatttgcatt gagcccaaaa aatagagatg atggtatgaa tggtatgaat
    11581 accttgccta ccattcgatt tgcgacgcct atatgcttcg aggatgcctt ttccgagtta
    11641 tgcagagatt ttatccatcg gggcgcagat ttattgataa atctaacaaa cgattcatgg
    11701 tctaggacaa aaagcgccca aattcagcat tatgctgtag cgcggcttag agcaattgaa
    11761 acaaggcggg cacttgtgcg ttcgaccaac tcaggagtca cctgcgtcat tggttccgat
    11821 ggaagaaata tagctgaatt gccccaattc attaacgctt ccatggtggt agatgttcct
    11881 atttatgaat ctcgttttac tttttatttg aattttggag actggtttgc ttgggtttgt
    11941 gctgatttcg ttggattgct tttcatctgg gcaattctgg atactaaaag aaaaaattat
    12001 gtaatacaaa gtaacataaa tcatttataa tattaaacag aatcactaag gaggattgcc
    12061 ttgagtattt ctagctattt tgacgcggaa gatttggaga tgcttgagga tgtatctgat
    12121 attgtaagat atcgagagca tcccgccttt gatgctgttc catttgtagg ttccataaag
    12181 aaacacccct atgataaaga aaaatttctt atacttttgc ttcacgaaca aaaaaacatg
    12241 ccatggttca aggaaggtga gattattgag attaaaaccc gagatgtgca agctttagat
    12301 gagcttccct caactattga cctcaatggt actgcgatca gcacattccg gatctggata
    12361 aggaggcggg caattgcagt gcgattcgaa cctttcgaag taaacgataa ggaatacaga
    12421 ccttttgata ctcatagcat ttcaagactt cttagcaaga gagattgatg atagacaatg
    12481 ggctttctgc agatagggcg agatactgga gaagcttaga taactcaaga atacaatgca
    12541 ttctatgcgc tcatgcatgc ataatccaaa atgaaacaag agggcactgt aatgcccgcc
    12601 aaaatcggtc tggcaagatg attcttccct tttatgcatg gatttcttct attgcaatag
    12661 atcctatcga gaaaaaaccc ttaaagcgat ttatgcccgg aacgtcaacc ttttctgtag
    12721 gattttggaa ctgtacaatg gattgcccat tttgtcagaa ttgggagatt gctcatcctc
    12781 cacctgcata tattcaaaaa caaatcattg agccagatag acttatagag ctagctctag
    12841 catcgggttg cccatctata agctttacct attccgaacc attattgcac atagaatatg
    12901 tcatagaggc gatgcagata gcacatagcc gaggtctaaa aacgatcctc gtgacaaatg
    12961 gaaatgcact tgatgaacca gctcgacata tccttaccca tacagatgct gcaaatgtcg
    13021 acttaaagac agccgatcct ctcatctacc gaaaattgct tggaggagaa ataaaggtta
    13081 tcaagaattt cataaaggtt gcttttgaac tctgccatat tgaggttaca tctcttgctg
    13141 ttccaggagt tctcgattca attgcacaaa ttgaagagat ctcaagtttt atcgcctcgc
    13201 tttcgactga gatacctctt catatcactc gataccatcc ggcatggaaa tacactaagc
    13261 cacctctttc tccggatact atacacgaaa tggctgaaag cgcaaggaat tacttgaaaa
    13321 acgtatattc ttattggtga atagcccctc ttgacagtct cgatttattt cggcattgta
    13381 agcatcgcca attacacggt gggtgtagct caggtggtag agtgccagac tgtggatctg
    13441 gttgtcgtgg gttcgatccc catcacccac ccgagcccga caccttacag gtcttgggcc
    13501 taaatctaat tgcgcgttcg tagctcagct ggatagagcg acggacttcg aatccgtagg
    13561 ccgcaggttc gagtcctgcc ggacgcatgt ttttgcatat atgggccgct agctcagctg
    13621 gcagagcagc agactcttaa tctgcgggtc gaaggttcga acccttcgcg gctcataaag
    13681 gccgaagttt ttcaattgat tttgggcctt gacgaagaag cttggttccg ctagtatgcg
    13741 cctcgcatga aattgcgaga gtggtggaac ggcagacacg ccagacttag gatctggtgc
    13801 cttaaggcgt aagggttcaa gtcccttctc tcgcaggatg ctttcctaat gaaaggcgtt
    13861 gctggtctga aaagagcctt ggccgcataa acagagcctt aatgcatgcg ggaatagctc
    13921 agtggtagag cgccaccttg ccaaggtgga tgtcgcgggt ccaactcccg tttcccgctt
    13981 cgacaacagg gcgattcgga gactggccga atcgcccctt tttttgcatt atagcacgcg
    14041 gtgtagcggg ctttttctat ttttacagaa gagtaaaaac tgagaggatt ccatggaaat
    14101 cagcgaaaag aaaattgaag agcttgagct ttctagaatg cgaatgacaa ttactgtacc
    14161 agcacaggaa gtacgatctg aatatgatac aatgatgaat gaatatgcaa aacaagctag
    14221 gatcgatgga tttagaaagg gacacgttcc tgtccctgtt ctcgagcgga aatttggcgc
    14281 atcgcttaag actgatgcta tggcacgagt catcgaaaaa gcactggaga ccgcccttaa
    14341 agatgtctct cgaccaccgc tcgcttatga aatgcctacg ctagaagacg acccaagctt
    14401 cgaactcgat tccgatttta tcttcagcgt tacctatgat acattccctg tattcgaact
    14461 tccttcgctt gatggaatag agatcgaagt accaaatgtc actattaccc aggaagattt
    14521 ggatcgagaa ttagaaaaca ttaggcagcg aaatgccatt gtggcggaga aagatgggcc
    14581 ttctgaaatt ggcgatattg ttaccgccag ttttgttgaa attgcagaag atggaagcga
    14641 aattcccggc acttcacgcc aggacttctc tttcgaactc ggcaaaaatt tgaacatcta
    14701 taaattcgat gatgaaatca tcggtttaaa ggctggagat gaaaaaatta taacaaaaac
    14761 attccctgat gatttcgagt ttccggaata cgcgggaaaa acagcgacca taaaggtctt
    14821 aatcagtaaa gtaaaacatc aagaccttcc agatctcgat gatgaattag ctcaggatgt
    14881 ttctgaaaaa tacaaaaccc tcgacgatct caaagaagct gtccgcgctc acctcatgga
    14941 agacctagaa gcaaagcttc gcaaaacaaa agaaaatgcc attatggatg aactcctcaa
    15001 aagaacgaat atatctattc cacgctccat gattactgct gagcttgcaa tgcggtggga
    15061 atcgctaaaa caacagacag gtgtcgattc cgatgagcat ttggaaatgc ttctggcaat
    15121 gtccggaaag actcgtgaga tgattttcca agaatgggaa cctctagcag agaaggcgct
    15181 tgcaacaaat attcttctcg ataagctaat agaagctgga tcttattctt cttccgatga
    15241 agaggtcgac gccgaaatag ctaaggaagc aattcatagc acaatgagcc cggccgagat
    15301 caaagcggag tatgaaaagc acggtacgat aaagtacatc aaagaagata tcgctatgcg
    15361 gaagtttttc gacaatattt tgtcttcagc aacgatcaaa gaatcggctc ccatgtccta
    15421 tttggacttt atgaaaggaa atgattaaat tttattttgg agctcgagaa ttgaatctcg
    15481 attatgacaa atcaatgggc gctaaggaaa tgaaatgtcc gaatatatga gcaatcttgt
    15541 tccaatcgta attgaacaaa ccggtattgg agagagatcc tacgatatct attcgcgtct
    15601 tctcaaagac cgaatcgttt ttatcgatgg cgaaatcagc gatattattg ccgatttggt
    15661 aattgcacaa cttctttttc ttgaatctca ggatccttct agagatattt cgatctatat
    15721 caatagtcca ggcggattag tgactgcagg gctagcaatt tatgatacaa ttcaatatat
    15781 aaaacccgat gtacagacaa tctgtatagg acaagcatcg agtatggctg ctctcttact
    15841 cgcctgcgga acaaagggga agcgctatgc tctgccctct tctcgtattc taatacatca
    15901 accatggggc ggagtttctg gtcaggcaag cgacatcagc cttcaagcac gcgagataat
    15961 tcgcttaaaa aagctgacga tcgactactt tgcgctacat tcgggccagc taatcgaaaa
    16021 agttgcccaa gatatggagc gtgatttcta tatgagctct caagaagctc tcgaatacgg
    16081 aatcgtcgat acgatcatgg agccgcgaaa gcatggcaag gaataagcaa ttcgatctgg
    16141 aacaagagac attctgctct ttctgcggga aaagctcatc ctatgtccgc aagatgattg
    16201 caggtcctgg ggtcaacatc tgcgatgagt gcgtccgtat atgcagccaa atcctcgatg
    16261 aggaagaaca gagagtaact gctaattttc ttacagaagt accaaaacca aaagagatca
    16321 aggaattcct tgatatgtat gtcgtaggac aagaatatgc gaagagagtc ctttcagtag
    16381 ctgtttacaa ccattacaaa cgacttgtgc ataaggtctc ggccccttct ccagatgtcg
    16441 aaatcgaaaa gtcgaatgta ttgctcattg ggccgactgg atcaggcaag accctacttg
    16501 cgcgcacact cgcacgtaag ctcaaagtgc cttttgctat ggctgacgct actacgctca
    16561 ccgaggctgg atatgtaggc gaagatgtcg aaaatatcct ccttaagctt attcaaaatg
    16621 caggtggaaa tattcaagcg gctgaacgcg gcatagtata catcgacgag atagacaaga
    16681 tttcccgcaa gggtgagaat ccctcgatta cacgcgatgt atctggagaa ggcgtccagc
    16741 aggcccttct caagataatc gaagggacta tctccaatgt tcctccccag ggaggacgaa
    16801 aacatccgaa ccaggaatat cttcgcatcg atactaccaa tatcctcttc atctgtggtg
    16861 gagcctttgt tggactagac aagattatcg aatcgagagt ttctagtcat gccatggggt
    16921 ttggtgcaga tgtaaaggca agacatgaaa aaatactaag tcagctatat tcagagctgc
    16981 aacccgacga tcttatcaaa ttcggcctta tccctgaatt tgtcggacgc ttgccggtaa
    17041 aggttgcgct ggatgagctt acaaaagaag acctcaaacg tatcatcgtt gaaccaaaaa
    17101 atgcattgct taagcaatat gaagcctcga tgcggcttga taatgtagaa ctggaatttg
    17161 acaatgctgc aatagatgca attgccgaaa aagctattgc tcagaagaca ggagcgcgtg
    17221 gtctacgcgc tattgttgag aagattatgg tggatataat gtattcgctt ccaagcgata
    17281 ataatgtaaa aaaagtaatt ttgacgcgag atgcggtaga aggcaaaggc agctgcatta
    17341 tcgaatatcg tcaaaaaaca gcctaagcgc aaaaatcaat ggggtggttg gctgttctcc
    17401 gttggagaca gcagcctgag aaatacccat cgaacctgat ccggataata ccggcggagg
    17461 gataagatga tgcgtaaaaa acaattcatc gcagttttcg aagtgatagc aatggtttcc
    17521 attgcagtcg ttacaggatg ggcgcaatca aatgctcagt caaaacaaca gaattctgag
    17581 ttgatcgtct ggacttatga ttcgtttatt tcagagtggg ggccagctcc tctaattgct
    17641 cgggctttcc aggaagaagc tggaataaag cttcgctttg tatcgaaggg agacggtgga
    17701 gcgcttttgg ccgcgctatt cgacccgata aacaagccta gcgccgatat aataatagga
    17761 ctcgatgata aacagctcga taaagcctta aagagtggcc tttttattcc actctcctta
    17821 aaaaacattt cagaaatcca gaaagattta ttgctagata caacaaatag gcttgtacct
    17881 ttcgatttta gccaatttgc gctaatctgg gactctcaaa gtggcatcga gccacctcga
    17941 tcattggaag atctgactaa gcctatttat cgcaagaagt taataattat ggatccaaga
    18001 acctctactc cgggtttggg ttttctagca tggacagaag cagtctacaa gactgattgg
    18061 aaagaatatt ggaaaagact tgctccatcc gtacttgcaa tgacaccgag ctgggatact
    18121 ggatatggcc tctttaccaa aggcgaagcc cctttggttt tgagctattc cacgagcccc
    18181 gcataccata aggcttatga gaactctgag cgctatagag cccttcaatt tgcagaggga
    18241 catccaaccc aaatcgaatt tgccggtgta ctcatatcta gccaaaagaa agaacttgct
    18301 caaaaattcc tagactatct attatctgcg aaatgtcagg ctttgctgcc tgaaacacaa
    18361 tggatgtttc ctgctaacat caaaactgag ttacctccat cattctctat cgtgcaaaaa
    18421 tatccagcct tgatagctac aataagagat ctcgatcatg atccttcgac ggcggcaaat
    18481 ctactcattg gaaatccatg agaacaagga tttttctata cctggtgcta tgcacaattc
    18541 tgatcttctc tttggcccct ctcattgcct tcttgcaagc cgcctttttt ggcttaagcg
    18601 aggcagaaag caagactgag gccattaaga cctttttaga cttctttaaa cgcataccag
    18661 aatcgacaat agcaaaaagc ctttggttca cgctaaagca atccattgcc agcactgctc
    18721 tagcaatagg ccttgggcta cctggagcgt ggctgacagc aagattccga tttccaggaa
    18781 ggaaggctct tctgtcttta gctgctatac cattttgttt tccgccaata ttgattattc
    18841 tcgcattcat atcttactac ggaaaagaag gactgctttc aacagctttt tcgctgattt
    18901 ttggttcaag aggagaatac agaggaattc tctacagttt ttggggacta gtcattgtgc
    18961 atgccttcta taacttccca attgttgtgc accagatttc tgcattattg ctgcgcattc
    19021 caaatactcc tctcgaagct gcaagaacac ttggagcaaa aaagctaaaa gcctttcgaa
    19081 caggtatttt gccttggatt atgcctggaa tcggccaatc agctggtcta atctttctat
    19141 actgcttttt tagctttact accgtgcttg tattcggcgg tcgcgctggg acaacacttg
    19201 aagtcgaaat ataccaagct ctacgatatc gttcaaatct agcccttgca gcagtctttt
    19261 ctgttattga gagcgcttgt gcgcttttag gaatatggat gctaacgaga attaagggca
    19321 agacatatag gacagtgaga gactttggag cggcacgcaa attggtaaaa cccaagggaa
    19381 agcaatggtt tctaatcgtg ctttatcaga tcttcatcat agtattcttt ataggacctc
    19441 ttttttcgat cgtaatcgaa gcattcagag ttccagcatc attgaatagc cctgataaat
    19501 ttggctttga taacttcaag cgacttttaa caggattcca agcgccatta actggagcaa
    19561 tcctttccac attgaaattg agcggaatcg ctgcattgtt agcgactttt atcggaatta
    19621 ttgcatgctt tagcatattc tttgcgcaga gaggagaaaa aaagagcaat atcgttgcta
    19681 tgatgatgga tctgctgcaa tggcttccta tggctgtttc gcctgcagtt attgcttatg
    19741 gatggctttt tatatctgca aaccatctag ctacttccgc ccttatcgct gctcaagcgc
    19801 ttattgcatg gccttttgtg agcagaacag tgggtgcatc gcttcgtgca atcgattcac
    19861 gaatgcacga agcagccaag acacttggag cctcaccaat aaaagctttt ataacaatag
    19921 agctgcgtac gattttgcct tcggtggcag cagccgcagc ctttgctttt tccataactg
    19981 ccggggatgt caatgtgcca cttatgcttg gaatgagcga gattgaaaca cttccgctta
    20041 ttctctatcg cttaaccgca gcttataggt tcaatgaagc ttgtgccgct ggccttgtgc
    20101 ttgggctcat gacgggtatt gtattcatgc taaaggagaa ggtcatcgat gtcgcttgaa
    20161 gtaaaggatc tgcttgtcga acgcaatagt tttgtgcttc gcgccgattt ttctttagag
    20221 caagggaaaa ttcttgtctt gctgggccct tctggctgcg gaaaaacaac tcttcttcgt
    20281 tcaattgctg ggctcgaaat gataaagcaa ggcactatta ccttgaataa tctgagaatc
    20341 gacaagttgc ccactgaaaa acgacatatc ggatttgtct tccaggatct agcactattc
    20401 gaacaactta gagtccgaga taatattgga tatagtctat caattcgaaa tgaagaaaaa
    20461 agaaagagag aagaaagggt catttccttg gctcgcaact tcaaaattga gcatcttcta
    20521 ggccgctacc cctatgagct ctcgggtggc gagcgacaac gtgtcgcatt tgctagggca
    20581 tttgctgctg atcctatgct catgttgctg gatgagccac tctcttcgct agatgcaccc
    20641 ctcagaaggg agatgcgacg ttttattcgt gtgcagcttt caagacagca gttgactact
    20701 atccatgtta cgcatgatgt ggaggaggcc ctggatttgg gcgacgaaat cattgtaatg
    20761 cgcgatggta agatgataag tagaggaata atgagcgatt ttgagaaaaa accaggttct
    20821 ggatggcttg ctcgttttat gaaccttggc cttgtgcttc ctataataga aatatcagcc
    20881 tcaaaagaga gccagctcct cgatataatg acccccgaag aaaatatgtt tcaagtgagt
    20941 cgcaacttag aaagcaatat cgataatgca ttggaaaaag cgatgtgctt gtatatacca
    21001 tttagcgcca taaagatttt tagaaagaac aacaatgaaa aaacaataaa tgtcaaaata
    21061 gttcgatatc ttacttcaac tcccctggta tccaaattag tcttaacact tccaagtatg
    21121 caggattact ttttcgagtt gcctatgccc gagtcggact ttccgatgcc tgcagaagga
    21181 acggaaatcg aaatttcaat cgatcagagt atgtgtcaag ttcttcctga tataccatta
    21241 tgagcggcgt cgatgagctt ttggatatcg attgcactct cttcgagcga atgattattc
    21301 tcccatgcat agcggattct tgctgcactc ctatagagct catggcgttg gcaatagatc
    21361 tcttctattt tcgattccca ctcctctgac tgaagaagtg gcctttgatg tctttggtct
    21421 tggagtcttc ttttcagttc ggctatcgat acatccagcc agatagtaag gaaagaattt
    21481 tttaggatca tgcggttaaa gtcgcgcaaa actacgccgc ctccacagga aagcacgaaa
    21541 gaacgaggag ttgcggccat tatggcgagc tgattttctt ctaaatctct aaaatattcc
    21601 tctccaaaat cgcggaagat tgccggtatt gtggtctttg tgttctcttc aattgatcta
    21661 tccatatcta taaaatcgat tccataattg cgagcaagaa gacagccaat ttcgctcttc
    21721 ccagagccca tcatgccaat caacgcaatt ttcttcatat agaaccaaat acatcatcga
    21781 agattcttag ccagtttcta cccagtattt tctgggctgc ttcatgacta taaccatgcc
    21841 ttctaagcgc gctatataga agcggaaact ctcctgcatc ttgaatcact ttgccttcct
    21901 gtggcaaaaa tccatcaaaa tcagaaccta tggcacagct ctcgatacct cctatgcgca
    21961 ctatatgatc gatatgaagc aagaaggctt ctaggctgca gtctttggga tcttttgtga
    22021 caaagtaggg aacaaacacg cagccaatag caccaccatg atcggcaatt gccctgattt
    22081 gggaatcgct taagtttctt ggatgatcga gaattccgcg agcgtttgca tgagatgcaa
    22141 caaatggacc agtggatatg tcagcaacat cccagaaacc ctcctcgcct agatgtgcca
    22201 catcgacgag aacatgcatt tcctccatct tctcgacaag ctctttgccg agcgaagtaa
    22261 ggccatcgct tccttcggct tttagacctc gaccaaaagc attacgtcta ttccaagtca
    22321 agccaatagc tctaactccg cgctgataga atatatcaag actttctagc gaatctccta
    22381 aggcttccgc cccctctatc gagagtatag cgcaaacgct ttttgtctct agagctctat
    22441 gtacatctgc gccatccttt gcaagaaaga attctttggg agcgaacttc aagagagatt
    22501 cgaacgaatc gatcatagcc atggtttcgc tggttgcctc ctgaagttgt gaatcttcaa
    22561 ggaaaatagc catgacctgg catacaacac gacctcggac tagcttaggg aaatccaaat
    22621 gcaccgaagt attatcagca aaaaaatcgc gcggatttct ctcttctttg ttcatgctcc
    22681 gacctgtgat ggccataagc gtatcgcagt gtgcatcgat cacatagaaa tcatcttggt
    22741 tattgttatt caacatatct aataccttta caaatctttt aattttgaaa tctcatcgcg
    22801 tattattgct gcttgttcga attccagatt ctttgcatgt tcgaacataa gtttctcaag
    22861 ctcttttatt agagattttc tttgctcagg aaccagcaag ttgtgtgtgt gcctaagcat
    22921 ctcgatttca ccttgagaag cttctattct ctcttcctta tggcgctcga ggatatcctg
    22981 gatcttttta ctaattgttc taggagttat cccatgttca agattataag cttgctgcat
    23041 ttttctcctg cgctcagtct ccgaaatggc attcttcata gctgggctta tgacatcggc
    23101 atacattaca acaaggcctg ctgcattgcg cgctgctctt cctatgatct gaataagact
    23161 tgtctctgaa cgcaagaaac ctattttgtc ggcatcgaga attgcaataa atgatacttc
    23221 tggcaggtct atgccttctc gtaataggtt tattcctacc agaacatcga attcccccgc
    23281 acgcaactgc gtcagaatct cgacacgctc aatagtctct acctctgagt gtatatagcg
    23341 gactcgaaga ccaagtccgc tcaggtaatc ggttagctct tctgccattt tctttgttag
    23401 cgttataata agacttcttt cgccttgttt agctctctcg cgaactcttg catagatatc
    23461 ttccatctgg ccttcggtgg gatgaacgat aatttctgga tccacaagcc ctgtaggacg
    23521 aattacctgc tctgctagag ttttggatct cttaagctca atctcgccgg gtgtggccga
    23581 aacatatatt gtctgtgaca caatcgactc gaattcatcg atcctaagag gcctattgtc
    23641 taatgcacaa ggcaatcgaa agccataatc gacaagcgat agttttcggc tatgatcgcc
    23701 attatacatt gctccaagct gaggcaatgt cacatgcgac tcatcgataa aacatataaa
    23761 atcctttgga aaataatcga gcaatactcc aggcctttct cctggcttgc gattggagag
    23821 tggcgcagaa taattctcta tgccactgca gtatcccatt tcactaagca tctctatatc
    23881 atattctgtt cgtgatttta tgcgctcagc ctcgagatac tttccatgtt cgataaaata
    23941 tcggtatcgt tcctccaact cattccgtat gctatctatc gcccgctgaa ccatgtcttc
    24001 cggcataacg aagtgttttg ctggatagat tattgcttct tctagttcat tcgatgcttc
    24061 acctgtaagc ggatctaagc gacgtatgcg cgcgatggta tcgaaatcta gctcaattcg
    24121 ataggcatcc tctagatagg caggaaagat ctccaaaaca tccccacgaa tcctgaagcg
    24181 accgcgctct aataccatat catttcgttc atattggagg cttatgagat tccgcttaat
    24241 ttcatcaata tctatgattt gacctctgtc taggtacaac ctcatctcac gatataggtc
    24301 aggcgaacct aatccatata tgcaagagac tgtcgctaca atcactacat ctcttcgctc
    24361 cataagcgaa gcggttgcag atagacgcat tcgatcaatc tcttcattga tatccgagtc
    24421 tttttcgata tagagatctt tggaagggac ataggcttct ggttggtagt agtcataata
    24481 ggagacaaaa tactcaacgg cgttctctgg aaagaattct ttgaactcgc ggtatagttg
    24541 ggcagataat gtcttgttgt gcgaaatcac gagggtgggt ttctggataa cttcgatgac
    24601 ttttgccatg gtgtatgtct tcccagaacc tgttacccct ttcagcgtca catacttttc
    24661 gcccttttta atggcttttg agatagtctc gattgcttga gcttgatcgc ccgctggctg
    24721 aaaaggagca acaaccctaa aaggcttcac ctctttcgct ccgccagaac aactgtagtg
    24781 ctcatctttt tcgagccgcg atagaattcg acagtaacgc gttgcccagg acttgtatct
    24841 tctagcgctg catatagttc tgcaattttg gtgacaggtt tcccatcgat actcactata
    24901 atatctccgc cgatatagaa cactgtcgat ctatagcgca ctgcagtagt accacctttg
    24961 aggcctgctt tttctgcatt gccacccgaa gttactgtcg atatgagaag cccagtttct
    25021 acaggtgcgg cttttccggt ctggcgtaga tagttgacaa gatctgggaa aagttgaatt
    25081 acctgcatct cgatccaacc tctcctaact tgtccattct ttatgagctg aggaacaatc
    25141 cgaaccgcag tgtctactgg aattgcgaat cctattccca ccgaaccgcc agatggtgaa
    25201 tagatcatgg tatttatgcc aatcatctct cctctcatgt tgaatagagg acctccggag
    25261 ttcccaggat tgatagatgc atcagtctgg atcatatttt gaagaataat agtttcgctt
    25321 tcctgaattg gacgccctaa gccagagata attcccgccg tcaatgtcct ctcaagaccg
    25381 aatggattgc ctattgctaa gaccttctga cctactttta gatccttcga tgttccgaaa
    25441 ggaataggtc taagcttttt ccccggttct ggagaaaact ttatcacagc caagtcactc
    25501 tcgggatctg taccaattag ctttgcctca tagcggctgc catctgcaag gctcacatag
    25561 agcttatacg ctccttcgac tacatgattg ttggtaagaa tatatccctg ctcatctatg
    25621 atgcttccag agcccgagcc tccactttga ggtacaggtt ctagaaacca attgatacct
    25681 aatacttcag tgttgatgtt gaccacagat tcgttgtatt tttcatagat agaaatattt
    25741 tcaatctcat ccgcactgta tccgctcata ctttgggctg aattcgcaat ttgcaaagga
    25801 agacctacag tttgatcatc ctggctacct aagctagtta tttcgggaaa atacacatct
    25861 tcctcggtat ctttggcagg ttgagaagcc ataatcatcg tagattggct ctgagcatgc
    25921 ttatcaataa caaaaacaat acctacaatc gataaggctg tcaaagccac agagacagca
    25981 atgaccatgc atatctgcgc ctttgaatat aacttcattt cgagtacctc cttaattctc
    26041 gctccttttc ccgatttctg ctgtcaacaa gcaaagctct tttatctagg atatcttaga
    26101 caaaggttag ctttccttgt gctgcttctt cttcgcgagc gagcatgtat atataatcag
    26161 acaatctatt gagagtgcgc atcaacacag gcgaaacatc ctctattctt gaaagagcga
    26221 ctacacgacg ctctgctctg cgagcaatgg ttctcgccaa atctaacgct gctgaacctc
    26281 ctgtcattcc tggcacaacg aaactcctta taggaattcg catttcaagt tctttggttt
    26341 tatcttcaat tgtcttttca tcatcttcgt tgataggatt atcgaaaggc atgctcagag
    26401 atgccaattc cccacctact ctaaatagaa ggcgctggat atactcgatt gcgcaaagca
    26461 cattgtcttg aatacagaga tgacgagcta ttccaagagc agaagagagt tcatcgatag
    26521 tgccataagc ctcaaccctt ggagaatcct tgcctatttt ttcaccagac cagaggccag
    26581 ttatgccctc atcgcctgtt ttagtaataa tgctcatata gtaaaattat acagactacg
    26641 aacaacaatc aagctacaaa aaggcttttc tagacagaat taatgcctta atagcgatgt
    26701 agcgcggctc aaggaaagag ttatgactac ggagaataaa gtaatccttt attttggagc
    26761 acaaggaaca tgcccttacc ttttcaataa tcatcaaact ctaatgaact tactagtttt
    26821 tcaagatgaa gttgaccctc gattatatag tgccttgata tcgactggat ggagaagaca
    26881 tggcaaaaat atctatcgta tggagtgtcc tagctgtgaa ctctgtatcc cattaaggct
    26941 tgagcctatg tcaatacaac tcacgttttc tctgaataga atcctacgaa agaacaagga
    27001 tatagtcatt atccctactc cgccaatctt ccgtgcagag cacttcatgc tatggcaacg
    27061 ctattctcta tggaaacact ccatacctct caaagacctc gcagaagact cttataacaa
    27121 tctcttagaa ccatggagtc taatatttga atatcgagat aataatgaag acagaaaact
    27181 tctagcagta agccatgtag atcctttaga tgacgggctg tcttctgttt atttctcttt
    27241 tgatcccgac gaaaaaggac ggagtctagg attttactcg atccttgctg aatcctacct
    27301 aagcagtttt gtaaaaaacg caaaatatct tttgccaaat aaggaaataa agggagaatc
    27361 agtttcttat ttttatgaga atagaatatc gtgtaaactg gaacctggtt atacaaaaaa
    27421 tcaaatcgca gcaatcggtt atgaaaatga gcattactat tatcttggct tttgggtgcc
    27481 aggagcaccc aaaatggatt acaaagcgcg aatatcaccc ttcgagatat ctattgccga
    27541 tgaaaatata gataatccaa atccttggaa gagatttttt tctaggaagg aagctataac
    27601 ttatctttca aggcgggaat ggccaggact ctcgttgact attccatagc ctcatgatag
    27661 tttaatgcag catttttata ggattatgga tgaaaggaca actaatggca tccgaagaaa
    27721 acgaaaaaca agcaaaaaca ggcaataaga aatccagcga aaaaaaaagc aataataagc
    27781 aagccaagac caccctcaag aaaaccataa agaatccatt tgtttatatt ggtacaataa
    27841 tcatccttgt gcttactatt attgctttcg tgcttattcc aagcattagc tctggcacga
    27901 gcgccagcgg taagattcct tcttttggat cgtggaatgg caatccaata cagtatacct
    27961 caggatctta ttttgccgag caggtatcgc aaataaatga ctaccttcga cagcaaggcc
    28021 ttgacgagac gcaatcacag ctctacgcct atcaagtatg gcgtcttgca tatcaaaata
    28081 cagtgatacg tacggcgatc ctcgattcgg caaagcgatc tggtatgaaa atcagcgaag
    28141 aaacaataga tgaagaagta tcgaaaaatt cccaattcca ggtagatgga aaattttccc
    28201 tggaaaaata caatagcacc tctgctgcga cccgtctttc tatcagaaat agaatccgtg
    28261 aagatatgct aattcaaaac tactacaatg atcttatgtc agcctctcct tccacagctg
    28321 aaattgaatt tgtagcatct atggcaaagc cacaacgcgc aattcagtat gtaaacatct
    28381 cctatgcgga cttccctgca gaggaaacgc tcgaatgggc taaatctcac gaatctctat
    28441 tcaggacgat tggcctatca aagatcacta ttagcacatc agaaaaagat gcaaaaaaag
    28501 tcttagccca aatcaaagca aatagccttt cttttgaaga tgctgcaaaa agtcattcaa
    28561 aggatatata tgccgacaag ggtggcgata tgggcactag gactttttat gatcttaaaa
    28621 gcgaattcct ggatgagaaa gatgcagaga atctcatgag tctcaagaaa ggtgagattt
    28681 caccagtcta caagcaaact ggtaacacct gggcttttta ccaaataaac agcgaatcga
    28741 gtcctcccga tttttcaaag caatccgtcc tagatgaagt aaaaagctat attttcgaca
    28801 aagaaaagag tgtgctggag tcttgggctc tggcaaaggc aaatgaattc aaagccgaaa
    28861 ccatgaatac atctttcgaa tctgcagcga aaattacagg tcttcaagta aagtcagcag
    28921 gtccctttat acttaattac ggcaatccag gtttctattt ctatggtcag cagatcagcc
    28981 tttttcagga accctatcgc tcgctggata ccgatcttgt tggcgcagta gagaacgaga
    29041 ctttccttac aacacttttc tcaacaccta aagatgcagt atctgaaccg gtacttctgg
    29101 atgcttcggt agtagttatg aaggttacgg aagacagtga agcaagtgat caggaagcct
    29161 cttacacgaa attctcttac ccttattttt tccaaaccgc catggaaaac catattcgta
    29221 attcaatttt atcatcagag aagttcaaag ataatttcaa cacgactttt gcgaagctct
    29281 ttatggctaa ttagttgata gtcctataca atagttagaa atagataaaa ggcttgtcca
    29341 tattcacgga cagcctttta tctatcctct tcttcactag cgcgcagtac agacaaaaat
    29401 acttcttttc ctagctcgac atttccaata gtctttaatt ttttcttgcc ttctttttgc
    29461 ttctctagta gttttctttt acgggttata tcccccccat agcatttagc aagcacatct
    29521 tttcgaacag gattgacagt ctctctagca ataatttgac ttcctatgga cccctgaatg
    29581 ggaatcttga attgctggcg tggaatattg cgacgcaagc gctcgcagac aatcctagct
    29641 cgatcataag catttggcct atatacaagc atcgagagcg catcgacagg ctctccattt
    29701 atgaggaagt ccagttttac aacatctgta agcctataac ccgtcacttc gtaatcgaaa
    29761 ctggcatatc cgcgggaggt agacttgagt ttgtcgtaga aatcaaaaag aacctctgca
    29821 agaggcattt caaatacaag ttctacacgc ttttcatcca aatgcagaaa agactcttga
    29881 ctcccacgct tgcctacgca gagagaaatt atattgccaa cataatctac tggggtgatt
    29941 atttctgcct ttatgtaggg ctcctctaca ctctcgatgc gcgaggggtc gggataatcg
    30001 ctgggattgt caatatacag tgttgagcca tctttaaaaa tcaccctata tcgaactgaa
    30061 ggcgcagtca taatgataga ttgaccatat tcacgctcga gtctctcctg aacgacttca
    30121 agatgcaaaa gtcccaaaaa tccacatcga aatccaaatc ctagagcaag cgaatggtct
    30181 ttttcaaata taagtgatgc atcgtttaat acaagctttt caagactatc tctcaattcc
    30241 tcgtaatctg aagaatccat tggataaatt gagctgaaga cgacaggctt tacctctcta
    30301 aaaccaggca aggctgtttt acagggattt tcataatctg ttaaagtatc accgacacga
    30361 acttctttta cagtttttat gcctgcaata cagtagccaa cctcccctgc cgagagctct
    30421 tctgctggag aaagcgccat cctaaaatgc cctacctctt ctacttcatg aattgcccct
    30481 gtagacataa gcctaatatg catgcctttc cttatagagc cttcttttag tctaagatgt
    30541 actatagccc ctcgataagg atcgtaatgc gaatcaaata tgagagcctg aagaggattc
    30601 tctgctacac cattaggtgg cgggattctc tctacaatag cttcacagag ctcatctatt
    30661 ccaattccca atttagcaga taccgcaaga gcactttctc cttccaagcc tagatcctta
    30721 tttatctgac ctcttacgcc ttcgatatcg gcactggcca tatcgatctt gttgatgact
    30781 ggaattatct ccagatcgtg ttctagcgcc atgtacatat tcgacagcgt ctgcgcctcg
    30841 acaccctgtg ttgcatcgat gactaagacg gctccctcac atgcagcgat cgctctagaa
    30901 acttcatagg agaaatcgac atgtcctgga gtgtctacaa gattgagcat ataggttttt
    30961 ccatctttgg ctatataagg aagactaata gcctgacttt ttatcgttat gcctcgctcg
    31021 cgctcgatat ccatgttatc aagcatctga tcttgaagtt tccttgaatc gatgagatga
    31081 gccttttcaa taaacctatc ggcaagagtg cttttcccat ggtcaatgtg cgcaatgata
    31141 caaaaattgc gaatgcaggc aggctcaatc ataggcaaac tttatacgaa acaagggctt
    31201 ttatcaacag attctaattt ctctccaagg atgccctata tctaaaaaca tcatttcgga
    31261 tatataatat gaaaaattgg agaggcctaa tgaatcttga aaatctttct ataatagaaa
    31321 aaggaggcct gcgaaaagac catgaaataa tggtgtacgc gctttctacc tgcatctttt
    31381 gcaagaaagc actggctttt ctcgatgcaa ataattttgc ctataaatat ctttttatcg
    31441 atcgtattcc attcgaatca aaaaacgcga taaagaagga gcttggcgaa agattcaaag
    31501 aaaatatcat ctttcccttt atggtaatag atgccagaaa acaccttatt ggtttcattg
    31561 agtcagaatg gaaaatggca ttgggagcct aagctatcga tgaaagatat ttatcttgat
    31621 tgggctgcaa caacaccgct tgattctgat ataatgcgca gcgcgctgga attcagtctt
    31681 agagcatatg gaaacccatc gagcaaacat tggcttggta aagaggctcg gcaggctctc
    31741 gaagaatcgc gtgcaagagt catgcactcc ataggtatta caaaagggaa ttttgttttc
    31801 accagcagtg gcactgaggc ggatcatata ccaatctttg caatcctcga gaggcttaga
    31861 cgtgctagtt ctgcaggaaa acccatacat gccataatat cttcgatcga acactctgca
    31921 attgaagaac aaatacgcgt tttggaaaga ctagggcttc aagtgtcgag gatcgatcct
    31981 gattcaaaag gacatctcga agctgagcac gtcgcttcta agatacgcaa tgagactgtc
    32041 attatctgca ttatgaacgt caacaatgag acaggtgcca ttcaagacat caaagccata
    32101 tcggagagca tcagagaagt tgttcagcta aagagaatga aaaagccatg gcttcatgtt
    32161 gatgcagttc aagcgcttgg aaagttatcg attagagatc tcgaaaacta tgcggatagc
    32221 tttgctgtat ccgcacataa aatccgagga ccaaagggaa caggtgggct ttggtttgct
    32281 cgacctccgg aaatttttat ccgtggcgga ggacaagagt cgggcttgaa accaggtaca
    32341 gaaaatctct ttggtgcatt tgctttttct cttgctgctg aaaaagcagc actatcgatg
    32401 ccagcaaatc aagagaaagc tttaatactt gaggaacgca tcatagatgg catatcgagc
    32461 attcccaatg ctctcataat tcctgaacga ataccaaaag ataatggctt tgtgccctcg
    32521 ataatatcaa tggctttccc tggtttaggc ggagaaacta tggtgcgagc attgtcggac
    32581 tgtcacatcg cggtttctac gggctcagcc tgcatgtcga acattcacca tgcagaacga
    32641 cgagtcttga aggctatgca agtacctgag gatatagctt tttcatcaat tcgtgtctct
    32701 actggtccat tgacaaccct acaagacatt gattcctttc tagaacaggt cgaggtcttg
    32761 tatcgaaagc ttaaaacctg ataatacaaa agctatgcat actcttttcc tcatcaaacc
    32821 aggtgaaatt gagctaaagc ttggcaataa gcgcgaattt gcgcgtcgct tgaaagaaca
    32881 gataaccaag aggctcaaag gtatcccatt tacgctcgag gaatatcccg gtagattttt
    32941 cctgactgtc gaagagaaag attcggaatt aactttattt gtgcttcagc attgccctgg
    33001 gataaatggt gttgcaaaag ccattaagac aaaaaaagat atagatgaaa tttgcctagc
    33061 tgcagtagaa tgcgctaaga cagaaatctt gacaggcgca cataccttca aaatagaagc
    33121 taggcgttca gacaaaagct ttccttttga ttcctacagg atatgcgtta ttgctggcaa
    33181 tagcgttctt gctgaatatc caacactttc tgtagatgtt cataatccgg attttataat
    33241 caggatagaa atcagagagc gagcctatat ctattctagc accgctgcag ggcctagagg
    33301 attgccggta gggagccaag gcaaaggaat tctacttctc tctggaggaa tcgattcacc
    33361 ggtcgcagga tacatgatgg cttgtcgagg ccttgcgctc gaaagcgttt attttcacag
    33421 ctatccttat acctcgcctg aggctcagca gaaagtcgaa cgccttgctg cacgtattgc
    33481 aatctggaca ggtggcatgc atctgtggat tgtaccattt acagatattc agcttgctat
    33541 atctaaaaca tcttatgagg agacaaaaac gctcatgctt aggatggcga tgatgcaggc
    33601 agctgatatt attgcccatc gcatcaatgc aaactctatc gtaactggcg aaagccttgg
    33661 acaagttgca agccaaacag cggaaaatat gcgattatct cagagcaaaa cttccttgcc
    33721 tgtcttaagg cctctaattg gaatagacaa agaagagatc atcgctatcg cgcgacaaat
    33781 agagacatat gagatttcga ttttgcccta tgaagactgc tgtgttgtat ttagtcctaa
    33841 acaccccatt ctaaagcccg attatgagga gcttaaaggc tattttgaat cgcttgcttt
    33901 aaataatcta attgaatcgg ctgtcatgaa atccgagagg aaatattttg atttcgcata
    33961 tgcgctaaaa actaatacaa aataaaaaag ctaccatgaa accatgatag ctttatctat
    34021 ccccaagggg aatcgaaccc ctgttggcgg gttgaaaacc cgctgtccta acctctagac
    34081 gatggggaca ttcatttcgg cattctaagc ccatttttag gacgggaagg tcgaacggca
    34141 gtgaacctat cagaatttcc aacgcatgtc aataagcaag accataattg aaatgcaata
    34201 agagtgcaaa cttagctttc aatgaggttc tatatgtctt ctttagccag atgctccatg
    34261 attttcttct gcaactcctg ggctagctta ttctcaggca ttatgcgcaa tgccagttcg
    34321 caatcggcat gagcctgtac gtaatctcca agctgccacc aacatagagc tcgtctatag
    34381 cgagaaaaga aatgatacgg gtgtatttcg agagccttgg agaaatcttc tatggcttgt
    34441 gtcagctcct tttgaatgct ctttacaact ccgcggtaat ataaggcttt atagcagttg
    34501 ggatctagca ttagacagct ggtaaaatca gctagcgctt cttcatattc agattgtgca
    34561 aaataggcca tacctcgatg tttgtacacc acggcagcaa gtgacttctc gggcttctta
    34621 tcgagaatct tggaatatat tgatatggca ctcttgaaat ctgaggcatt atgtgcatta
    34681 agcgcagaga gaagcagcat atccatgtca agcccattcc atcggattgg agctgaaata
    34741 ttgttttcat gaaaagtagc tgtctctccc tgttctatcg tctgagctga aaatggagaa
    34801 agaatttctg ttgtcgcttc agagattccg aagatttctg ctgttttttg ttcgattttg
    34861 ccataaaatc cctcacgacg cttcttaagc gcatcgctca gttttttttg ataatccaat
    34921 atttcctgaa aaatgatatc ggacagcgta agcgtggcgt ttagagcagc cagtttcctt
    34981 ttcaagggct cgtcgagcgg agtgaattca gccttgtaga caatttcatg ttctacctct
    35041 gcccaagctt cttgcagaat ggtcctcaac tgaatctcta ttacatatct ttccaagcct
    35101 tcacagagag gaacaagctc atcgggaata cgaatcagca catggattga ttcataccca
    35161 aattcacgaa acgaacggtc ctgtcctttg tgctcgattt caacaacctc ataacgctgg
    35221 cctagaagag aaacaacttc ttccagatcg tgcaaaaatg gacagataat tctcatagca
    35281 agaacatcgt tgatgggtaa gggattcccg tgttcgtccc atgctttctt aagtagcctt
    35341 attcttttag aaaaatagga ctcaaaatct ttgaccctac ttttgatcgt tggatgtatt
    35401 cccttgtctt gtaagagttt ggaaatatca tggactgtgt ttgctaaagc ttcctcatag
    35461 ccagccctat gttgattgta gagcttctct aaaagagttt tatttgggat attctcaaga
    35521 tcattcataa gaataaagtt atcatgaaac atttcaatcg gcaaaggagg gggctacttt
    35581 atcacttgtt tagattctta gttcgcttgt gcatgagtgc attagagcaa aaaagaataa
    35641 tattgcgaac atttacatct attgcatcta tttatccaga tagcagaaaa attgaactca
    35701 tcgcgcaaga atgccattta taaatatata gatatgaaaa gtccccggta tcgtctcttt
    35761 aagactcttt tccaacgcgc tcttgaatgt cttatactct gaagatagtc ttgccacatc
    35821 ttgtgttgag aaatccagaa aaagagcatt cttcccctca actattcgaa gcagcttgat
    35881 atctaaatat gtcaaaggct taagatctct agaaagagga ccaagtaaaa gttcttctat
    35941 agtagcttgg gctttttcct ctaaagtccc tattggcgct attggccgct tttccagctt
    36001 caattctcct tttttcttat ctggaaaata gagacgcata caattgtatt gaagtgttgg
    36061 tcctttccat acaatgaata ggatagtaag gaaaattatc agggcaacaa tatagccttg
    36121 cttatggtct tgttctttag acggattcat atcattgttc gaaatgttcg ataaagtcac
    36181 agacaccatt gtatatggca tctgcgattt gtctcaagta accagcctga gataaaagaa
    36241 gagcttcaga cttattggtc acaaagccta cttcaatcaa aatagaaggc atttttgcat
    36301 ttcgcacaac aaaccattcc tctgcgcgga tccctcgcgc tgggcttcta tcaccgatta
    36361 tcctgtccat cctctgatat actttctgag ctaagattat gctctctgta gtaaattctt
    36421 cctcaagcat gatatttatt atgggagcta tatcctcccc tttttctttt gcggtttttt
    36481 catccactac agtacgtcga taatttggat ttaggtacca gacttcaaaa ccttttgtat
    36541 tcttgttaaa agaggcattt gcatgtatgg aaatatagat tattgcctct gtctcttcca
    36601 attttatctt gttagccatt tcgacccgtt cttcgagagt tggataaata tcgctcgagc
    36661 gggtaagcgt aatcgtcctt tcagggtatc gaagacgcaa taaatcagcg agtctaaggg
    36721 caatactgag attcacatcc ttctcttgaa gcctaaaatc ctcaaattct cctatagctc
    36781 ctggatcttt gccaccatgc ccaggatcta tcacaatagc ttttactaca tactttgagg
    36841 atgtctgtgg ttttaattcg aaaaattctt gtactgtctg tgtaaattca tttttaattt
    36901 gcagagatgt gactgattcg taaggtgcgg gcacttcttt tacaattgtc cagtcgagaa
    36961 gtacataggg aacatcgacc gcaaaacgca cactgcgacc ttcttttgta atcgttccag
    37021 catgaagtaa tggatcatat tccagcgtag ccttgagcga ataggctaga tcagaaatac
    37081 taatgtcttc ttgttttttc tcatttgccg caaaaagcgc taaattggat agaacaagca
    37141 ttgctatgat agcgatagtt ccaggcagaa tctttaggca aaattttttc ctggatatta
    37201 aaaccaacat catatctgga agcttataca tttctgctta acaatagcaa gaatatgccc
    37261 ttctgtcatc ccaaagccaa gttatccaat atgctgaagc aagctaaaca tcgaagaaaa
    37321 ataccaaatt actacaagag ctattgcatt tagagtttcc ttgagtatat aagataagaa
    37381 aagacaccac tacatattgt agtatcgtgc taatatgcta gtatagatgg tatggtttac
    37441 ttcaaggaga caggatatga aaatagaacg cttgtttact aaagcaggct atggccctta
    37501 tgaaaagatc aaatgggaaa agcgcaaaag tgagattaga aatcccgatg gttcaattgt
    37561 tttttcgatg gattctgtca tagtaccttc attctggagt tccatatcaa gcgatatcct
    37621 ggctcaaaaa tactatcgga aagctggagt tccaaaagac aaagccatcg aatggaaaaa
    37681 gtttatccct gcctcccaga ctgccctttc taatgaagat atagtcgatg atgccgaaca
    37741 cgatgctcgc caggtattcc ataggcttgc atatacatgg tgcgattggg gacaaaaaga
    37801 tggctacttt gattccgaag aagatcgaca ggctttttat gatgagatct gctatatgct
    37861 agcccatcag attgcagcac caaatagccc ccaatggttc aatacaggac tattctctgt
    37921 ttatggcatc gaagggccag ctcagggaca ttactttatc gatcccaata ctaaagaggt
    37981 ggcaaaatcc gagtcagcct ataagcgacc tcaaccacat gcttgtttta ttctcgatgt
    38041 aaaggatgac ttagtgaatg agggcggaat tatggacctt gtaacaaaag aggctcgttt
    38101 atttaaatat ggttccggta cagggtctaa tttttcacgc ttaagagctg ccaatgaaaa
    38161 actctccgga ggcggcgttt catcaggcct gctttcgttt ctaaaaatag gtgatagatc
    38221 tgcttcagcc ataaaatctg gtggtacaac taggcgcgca gccaaaatgg ttatcctcga
    38281 tgtagaccat cctgatattg aagactttgt gcgctggaag actgaggagg aatataaggt
    38341 agcatgcctt gctgcaggca gcggcctttt gaatcactac gcacaagtaa ttatttctgc
    38401 gatcgaagac atgggagctc aagatgaaaa cagctatctg cctgatcgaa atctactctt
    38461 acgtaaggct ataaaacagg cgctaaatgc aggactccct tcatcatgga ttcatcaggt
    38521 attgacctat tatgcacaga caggcaaggc tcctcgaatg ccacaataca acactgcttg
    38581 ggaatctgaa gcctataaca ctgtttctgg tcaatcatct aataattccg tgcgggtcac
    38641 caatgagttc atggaggcag ttatcaatga tctcgattgg gagttgatag cgcgaactac
    38701 tcagaagcct ttccgtgtaa caaaggccag aaatctttgg aatgaaattg ccgaatcagc
    38761 gtggttttgt gctgatccag gtctgcaatt ccatactact atcaatgaat ggcatacctg
    38821 tgttgccgat ggcgagatcc gcgcatccaa tccatgctcc gagtacatgt tcctcgatga
    38881 tacagcttgc aacctcgcat ctttgaattt gctatctttc tatgatttta aggcaggcaa
    38941 attcgatgaa atggcttatc gccacgcgat tcggctttgg actcttgttc ttgagatttc
    39001 ggttgcaatg gcgcaattcc cctccaagga aatagctaaa aacagctttg attatcggac
    39061 tcttggatta ggatacgcca atgttggaag cttgctcatg gttatgggtc ttccttatga
    39121 ttcagagaat ggaagaactg taactgctgc tctgtcggca attcttactg gtgaggctta
    39181 tgctcaatct gctcgcatag cgagagagtt tgggccattt gctcgctata aggccaataa
    39241 agaatctatg cttagagtga taagaaatca taagcgcgca gcatacaatg cagccaagga
    39301 ggaatatgaa gcgctgagcg tttttcctaa agggctcgat tcacagtcat gtcctagtaa
    39361 tcttctaaat gcagctcatg aatgttggga cgaagcgatt caactaggag agaagtatgg
    39421 ctttagaaat gctcaagtga ccgctattgc accaactggc accatagggc tcattatgga
    39481 ttgcgacact acaggcatag agcctgattt tgctcttgtc aaattcaaga aactttcggg
    39541 tggagggtat tttaaaatta tcaatgcctc ggttcctcct tctcttcatg cgcttggtta
    39601 ttctgaagac gaaattgcag atatcattca atatgcttta ggaaagggaa cactcgaagg
    39661 agcaccggga atctcgcttc agtcgctagt cgacaagggt cttccaaaca attcaatcga
    39721 tagaatcgaa aaagctctta aaaatgcaat caatctggat agctgctttt ctctatataa
    39781 tgttggctat gatgttctag aaaaattggg aatatcgaaa gaggaagcga atactccggg
    39841 atttagtctt ttatctcgct taggctatag cgatagggaa aaagaagaag ctgaggttta
    39901 tgcctgtggg acaatgggcc ttgaaggcgc tcctcatctc aagtcagagc atctagccgt
    39961 cttcgatacg gcaacaccct cgggaaagag aggaagccgc agcatatcat ggcaggcgca
    40021 tatagcaatg atggccgcag ttcagccttt catcacggga gctatctcca agacaatcaa
    40081 catgcccaat aatgtaagca tagatgacat taaaggtgcg tatatgcttg cttggagatc
    40141 tatgctgaaa tctatcgcta tctacaggga tggatcaaaa ctctctcaac cacttgctgc
    40201 tcttacacca ggctcaaata tcattgctga ttcgattata gctcttcagt caggtgaaac
    40261 gactctagaa gatgtcgctt ctctggaagg cacctcggat agttcaacta aaaagaatgt
    40321 cgagccagaa cttcccggtc ttcctgtggc accgcgcggc ataagaagag ctcttccaaa
    40381 taggagaaag ggttataccc agaaggcaaa gataggtggt cattccatat tccttagaac
    40441 cggcgagtac gaagacggca gcctcgggga gatctttctt gatatgcata aagaaggcgc
    40501 tgccttcaga agcattttga attcttttgc aatagccgta tccttgggtt tgcaatatgg
    40561 tgtgcctcta gaagaatata tagacgcctt tacatttaca aaattcgagc caaatggaat
    40621 agttagcggg catgactatt taaaaatggc cacaagcgtc ctggatttta tttttcgcga
    40681 cctcgcaatc tcctatctac atcgctatga tcttggacag atcaagcctg aagaccttat
    40741 tgcgacatcc acacaatcgc tttccaataa gacttcgagt gccttcgaga gtggcaagag
    40801 acacattccg acagaaagag atacgaatat tccacttatc agagaggcta ggcaaaaggg
    40861 gtatgaagga gacgcctgtc ctgtttgtgg taaccttacg cttgttagaa atggtacctg
    40921 cctcaagtgt gagacatgtg gatcaaccac tggctgcagc taattcttaa gtataagtaa
    40981 atcttgtggc ctataatcga atcaagaaat agattgatat ggtaaactca ccttaaagtg
    41041 tatatgtatt agctattatt cgacaaatcc tgcaaaaata cctaagaata aggctacaaa
    41101 atcgtatcat tattcatttt aatgcttgtg gtataaaaag ttatttttac ctcaccttat
    41161 tgactcagac acgatagtag gcaaaaataa atatatgtct tagtcattct taagacaaaa
    41221 tgaaagaaag gagcttagat gaaaaaacga aagataataa tcatcggggc agcagggagg
    41281 gattttcata acttcaacac tcgttacaga gaggatgaat caaccgatgt tgtggccttt
    41341 acggccgcgc aaattcccaa tatcgatgga agaagatatc cagctgctct tgctgggcga
    41401 ctataccctg aaggaatacc tatttattcc gaaagtgagc ttcccgatct gattaaaaaa
    41461 tatgatgtcg atgaatgtgt attctcttat tccgatgtac cctattctca tgtcatgaaa
    41521 atgtcggcga tagtgaattc tgccggtgct tcctttacct tgcttggtcc taaggacact
    41581 caacttaaat cgagtaagcc tgtcatatcg gtttgtgcag tacgcactgg ctcaggcaag
    41641 agtcagactt cacggaaaat agtccaaatg cttatgaagc gcgggcttaa agtcgttgcg
    41701 attcgccatc ctatgcccta tggggatctt gtgcaacaga aagtgcaacg tttcgctacg
    41761 atagaagatc tagcccgtta tcactgcaca attgaagaga tggaagaata tgagcctcat
    41821 atagcccgcg gcaatgtcat ctatgcagga gtagattatg aggcgattct tcgccaagca
    41881 gagaaagaag ctgatgtgat tctctgggat ggcggaaata atgacttctc attctatatt
    41941 cctgacctgc aaataacggt cgcagacccg ctgcgcgctg gcaacgaggt atccttctac
    42001 cctggcgaag tctgcttaag gaccgctcat gtcgtaataa tcaacaaaat tgattcagcc
    42061 tcaccagagc aggtccaaat agtaagagat aatattgcac ggacaaaccc gtcggctatt
    42121 gtaatcgatg gagcgagccc tatcactgtg gatcgacccg aattgatccg tggcaaacga
    42181 gtcttatgtg tagaggatgg accaactctt acacatggca tgatgaaatt tggagctggc
    42241 gtggtagcag ctcgtaaatt ccaagcttct cagctcattg atcctcgtcc atatctaagt
    42301 ggaggattaa agcaaacatt caatacttat cccgatatag ggacacttct tcctgcaatg
    42361 ggatatagcg agacgcagaa gcgagatctt gaagatacaa tcaatcgaac cgattgcgag
    42421 acagttatca tagcaactcc tatagatttg aatcgcatca tccatataaa caagccaact
    42481 gttcgtgtcg attatgagct tcaagagatc ggatcgccga atctcgatga ggttcttgag
    42541 gacttctgta aaagaaagct ataacttgag acaataaaaa tccgctcaag gagacggttt
    42601 gatcgtacag aaaaataaaa ggggctgccc aacttgttgg gcagcccctt ttaaggttta
    42661 ggacctcagc cagcaataat ggcttctgtc ggacaggccg cggcacatgc tccacaatcg
    42721 atgcatgtct ctgggtcaat gtagcgagca ttgtcttttt cgctaattgc gcttactggg
    42781 cattcgcttt cgcaagatcc gcaattcgtg caagcttccg tcactctgta cgccatgtga
    42841 aacctctcta aaaatttgat tattccttga aggatatttt attattttat catcaagcac
    42901 ctaatatggg aatgggtttc cagataatat aaaataatgg cttttatata gatagatatt
    42961 gagcgatatc tttgattcca tcatatagct tccattcgtt tcgacatcca tcctgctgtg
    43021 gaagacatac taaaaaagct tcaagccaca atctattaga gtcttgttct ccatacagca
    43081 tatcccctag aatgggcaaa cctatccatg cgctatgagc tcgaatttga tggcgaaatc
    43141 ccttatgtat ttcaacttcg aggcatacta ccccatttgg cagttgccgt tttacattgc
    43201 ttacttccat cactttataa gcagcaatat atttgctgat atatatctct tttgtgattg
    43261 gcttattgga ttttgacgca aattctggcg cgatacagga caccaaagca cctttgggac
    43321 catagggtcg aaaaaagctt tctatatata cttctttatt ggataggagt tcttcgataa
    43381 aaaatgattg cctctctttc attgttttag gcaaagatcc tggaagttcg cactctgaag
    43441 gggctgaaat tgaaatataa cgctttgcaa tggcaagttc attctgcgct tgccgaagtc
    43501 ttataaagct atcccgattc gctgcaaaaa gcagaatccc agaggtagcc tgatctagcc
    43561 tataaacaag tccaagttct ctaccaacta gagacccttt aaggccaggt attctctgat
    43621 catatagcca agatactata gagtgatcac ttttcttttg gcttacagaa tggaaaccac
    43681 atggcttata gacagcacaa acccgatcat cttggccaat tataaagggt tccttctcct
    43741 ctgcaaatgg catcatttat tccgcttcct tattctgaag cctatataga ttccagtaca
    43801 agcctttaag agcaatcaat tcatcatgtg tacctatctc taccacacga ccttgtccta
    43861 gtactatgat cctattagaa ttgcgaattg tggaaagtcg atgcgcgata actatgctcg
    43921 tgtggccttt taagagacct tcaatgcctc tttgaagaag cttttctgtc tctgtatcta
    43981 tggaacttgt agcttcatcg agaataatca cagatggttc atgggcaagg actcttgcaa
    44041 aagagattag ttgtctttga ccaagcgaaa gatttagacc cccttctgca agtggagtat
    44101 cataaccttg aggtaatgat atgatgaaat catgggcata gacagccttt gcagcttctt
    44161 ccagcttttc ttgtgaaaga tcgagcccaa ggcttatgtt ctctcgaata gttccttgaa
    44221 aaagaaaaac atcctgagga acaggttgaa taagtcttcg aatttgcttt aatgggaatt
    44281 gtcctattgg cactccatcg atcaaaatag acccttcttg gatatcccag aatcgagcta
    44341 ataaatttgc tacagttgat tttcctgctc cagtatagcc gacaattgca actttttcac
    44401 caggatttac aataaaagat acatctttga gcacccattc ttcattttta taggcaaacc
    44461 acacgtgtga aaattcaata tgcccgcgaa tagttctagg aaaaggcttt cctacaaatt
    44521 ctggtaactg gtctggaagc ctttcatctt ggtccaaaag accaaatatt cgttcaccgc
    44581 tcgccattgc gctttgcaag agaatatatt tttccgatag atccttgata ggagagtaaa
    44641 acattgaaat aagactgata aaagcaatga gtgtaccaat agatatctgc attctcagaa
    44701 atagcatcgc accaaaaaag attgctagaa ctgttgttag ggttgccata aaatcgacaa
    44761 aaggtctgaa ggtggcatag acatacatct cggcaatatt ggcctttaat agctgtttat
    44821 tgtttttccc aaagccctca gaaactttat tttcttggac gaagagcttg actatatcta
    44881 ttcctgatat atgctccgag agaaaagaat taactttact cgtccaatag cgttgatttc
    44941 gaaaagcatc gcgcgccttg atacgcgcaa ctgctgaagc gataaagact aaaggcattg
    45001 tggctagtac gacaaatgcc agtttccaac tcagcaagaa aagtacaata agagatccta
    45061 tcatgataga agcatctttg atgaaagcgc ttaaaacatc gctaaagaat tggctgataa
    45121 cttcaatatc gctggttaat ctcgtaacca agcgccccac tggctgcctt gaaagataag
    45181 aaagcgattg ttctatcaca tggtgaaaaa gctcgagacg catgtccttc atgatcaagg
    45241 tagaaatctg attcgtgaac caagtcatag cgaaggttgc accaagtacg atcaatagca
    45301 caatcaataa aatgagacca aagcgcaaaa gtgctttatc atccgctgct cggagaatcg
    45361 cagcatcttc agatcttaat gtcctcaaga ccgaagatgg gatcactccc cagttgtccg
    45421 aaagctctac tttagagatt ctctctgcaa tggagatctt ttcctcgcgc attggatcaa
    45481 gtggaaacac ataactcgct tcattgtcta gaagaccttg ttcgaaaaga cgatttcttt
    45541 cggccattgt gagtcctgcg aggcgagaag cgcgcacata tatgcgtcct ccgatcctaa
    45601 cttcgagaga accctcgtct ttaggaagga tatattttgc tgaaggatca ataccatacc
    45661 aatccctaac aactacatca tcgatggcac gctttatgat agtaggagat atgagttctc
    45721 cggcagtggc tattgcgagg gctagaatag ctattatcgc atagagtcta tagggtttta
    45781 gataaagaag aattcggcgt gcaatctggg catcatagct ttttgtgacg ggctctgcgt
    45841 cgaagtattc atccatagat tgtttcctct gacacagaag catcatgcgc taaagacttt
    45901 tgctctactt gcgtttctct ttgagaaata ctatctccaa tagaagggat agaagcctgc
    45961 tgttcaagct gctgcaaggc tgcaattctg gcatagaaac cttcgctatc tgctattagc
    46021 tcttcatgtg aaccttgctg gattatttca ccactgtcta gcactatgat atgatcagcg
    46081 ttgcgtagag tcgatattcg atgcgaaaca atgatggtag tcttgccttt tctttcatca
    46141 atgagagaac taagaatatg ctcttcagtt tctgcatcca cagctgacaa cgcatcatca
    46201 agcaagagaa tatatggatc agctgcaaga gcacgagcca gggcaattct ttgtttctga
    46261 cctccagaaa gtgttattcc cctttcgcct acaattgtct cccatccatt ggggaaaagt
    46321 tgatagtcat gatcaagtcc agatatccta gtaatggctt caaagcgttc gggactgata
    46381 ttgtcggcag aaaaaaggat attttgcctt atgctatctg agaataaaaa gctttcctga
    46441 ggcaccatac cgaaagcaga ccgaagttcg ctcagcccat agaaacatat atctatattg
    46501 cctataaata catgaccttc tccgggttcc aacattcgcg gaagtatttt taagagcgtg
    46561 ctcttgcccg atcccacttt tccgagaatt cctagcatag aaccttcatg gatataaaca
    46621 gaaatatcct tcaatgcggg cttttgcgtt cctggatata tgtaattaag ctttttaatg
    46681 tctatattgc ccctgggcat ctgttttaag caattcgatg attccttgat ctccggctct
    46741 gttctgagaa tttcatttat tcttttcaaa cttgcagccc cgcgttgtat gatattcacc
    46801 gtgaaacctg cactcattaa cggccatacc aacatttcaa gatatgacag catagctatg
    46861 atcgaacctg gagtcatttt attccgaatt gaagccctac caccaaaaag tatgagcagt
    46921 actgtactca atccagaaag aaaagtgata aaaggaaaaa agaagccaga agttttcact
    46981 agatccataa ttgcattttt atattctgta ttcgctattg agaatttttt gaagaaatac
    47041 tcttctttta caaaggattt gacaattcgt attccagaga gggattcctg ggcgatatcc
    47101 gacaaatgtc cataaatatc ctgaattttc ttgaacagtt tgccgactat tcgaccaaag
    47161 agcaaaatca gagcagttat taaaggcaaa ggaagtatta tccaagcagc aaccgctgaa
    47221 ttgtttgcaa acattgcggc tataatcatg cctgtcataa aaataccatc gataagagat
    47281 acgaaaccca tacctgttgc ttggcgaatt gtacttatat cgttagttgc ccttgccatc
    47341 agatcgcctg tagtattctt tctaaagaaa ttccccgaca tggacaggat atgtgagaaa
    47401 agcctatctc gcatttctgc ttcgattcgt ctgcttgcaa tattgataaa atatcgccaa
    47461 aaaaatcgtc ctatagatat taaaagagca agcgacaaca tatggaataa aggactaatg
    47521 atgtcagtaa aaacaaaagt tccgctagta atgcggtcta tggccatttg aatatatcga
    47581 ggaatgagaa cctggcttgc atctactata ataagacata agatgccgcc aacatatcga
    47641 tatctataac ttcttagatg aggcaacaat gttgagtatt ctttaaacat atatttcgat
    47701 tctaacgatg gcttagaaac tcagcaagca gttgcttgaa tcagaatctt ctgccatatt
    47761 gcatttatga gccaggaaga tttcattgta tatacagatg gtggatgtaa aggcaatcct
    47821 gggcctggcg gatgggccta tattatgcgc tatggtgatc gatatcgaga agcctggggt
    47881 ggagatcctt ataccacaaa taatagaatg gaacttactg ctgttataaa ggcgctatct
    47941 tttttaaaat caagaataga agatgctagt tcttcggcct ctaatagctc gctggaatct
    48001 ccaccatctt ggacaaaagc acaaatcaat atattcaccg attcgatgta tgttaaaaat
    48061 ggtattacaa aatggctcgc agaatggaaa aaacgtagct ggaaaacctc tgcaaaaaag
    48121 cctgtcatga atcttgagtt atggcaagaa ttggatagcc tttgttgtaa gcttgaacct
    48181 catttcgagt gggtagcagg acatgcaggc aacatcgaca acgaacgatg tgacaaattg
    48241 gttcaccttg ctatagagga tatgctccgc cagaaagaaa gtgggacttc gatagttgtg
    48301 ccactatgat tctttggttt aaatttataa cttgactttg acagtttgtg ttctaaaaac
    48361 acgttataat ctcttatttt ataat
//
LOCUS       DAOSOB010000008        43623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_919_length_43623_cov_58.030275, whole genome shotgun sequence.
ACCESSION   DAOSOB010000008 DAOSOB010000000
VERSION     DAOSOB010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 43623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43623
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_919_length_43623_cov_58.030275"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /gene="secG"
                     /locus_tag="PLS88_01690"
     CDS             <1..332
                     /gene="secG"
                     /locus_tag="PLS88_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOU06099.1"
                     /translation="VIIQDEESDSIGGIFASGAQSAFGSRTSNIVVRITYVLGGLFFV
                     IAFSLAILNKSSTGNIQKVVEQESTQTATSEWWNSQGDGDQSVQSQLAPQDQTTQPEG
                     LNTPATK"
     gene            479..877
                     /locus_tag="PLS88_01695"
     CDS             479..877
                     /locus_tag="PLS88_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06100.1"
                     /translation="MRIGINAIGSRRLSSVSNAMLHSLKEKGHDSILTSEYSYSLGSF
                     DFLIFLTEPKGMFGGLLSSVAQDLAKSGDLVGKRCLAMVQKRGFRSSHTLKKFMEALE
                     HEGLVIVQGEVFSNIDTAIHIIAEVPLKRG"
     gene            881..1735
                     /locus_tag="PLS88_01700"
     CDS             881..1735
                     /locus_tag="PLS88_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOU06101.1"
                     /translation="MKNYYEILGVEQDSSQKDIKSAFRRQAKRLHPDMYYSTGKARSE
                     ESPATLRESAMRLILEAYKILSDAEKRRIYDRELQRKEKENKGFDYREFLKMRADDPQ
                     SQAKLIVFDLLHGFEEEAIWIYERSKSFQDFRLERWLERGEAMDCEYCIAEEYEKRGK
                     YIKAYQIYKKVIQMEMEKPWFRYYFDVVALQFRLLVLQKLPGKIDEDDYLDRLDEAIE
                     LGISPRETAQYLRKKVEILVHRGEADRAAETLMQISQIYPKLAGFDALHAKVQRALEQ
                     QVAQDRVY"
     gene            1789..2820
                     /locus_tag="PLS88_01705"
     CDS             1789..2820
                     /locus_tag="PLS88_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU06102.1"
                     /translation="MKRIATCFLVFLFTAISAFAQTSIDKPAATIKLVKQEVISVRQL
                     RTDVEKLENSVGVKLTLDQRKDVLDARINSMLFLQFCEREKISVSEAQVNAALSQLRS
                     QLGTGATDADLEKSLRASGVFVDPATFVRQRLLFETYIQTKRQDELKVALKAPTADEI
                     LKAYDLAKASLVRPDTIRISVIYVDTKGKTNAEIAKAKELINSISVSLKSNPTKFDEY
                     VLRASDQAGYKAIPSMYLEKTQQSKSIFGDEFFNAAFQANVGEITPVIETPVGYRIVR
                     VNEFLAQKQLTLSDPVPGNQNLTVQEFLAIQLASEKQQAFLNKAEADLIEALRKEATI
                     KIYSENLNW"
     gene            2833..3252
                     /gene="nusB"
                     /locus_tag="PLS88_01710"
     CDS             2833..3252
                     /gene="nusB"
                     /locus_tag="PLS88_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOU06103.1"
                     /translation="MASRRKARILAVQAIYAWDMSHQELEDILSFEWLDENKKARYEI
                     EILDFAKLLIIGTIEQIDSIDDLIKKHLEHWTFERLRKVDLAILRVGTYSLVFQQDIP
                     AQISIDEAIEIAKEYGSEESYRFINGVLDGIRKDFFG"
     gene            3257..4003
                     /gene="map"
                     /locus_tag="PLS88_01715"
     CDS             3257..4003
                     /gene="map"
                     /locus_tag="PLS88_01715"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOU06104.1"
                     /translation="MIRIKNERQIEGIRSSCDLLSLLYEKLKLQVQPGNTPKDLDRFA
                     YDFIIKHGGKPAFLGYEGYPATLCISVNEVVIHGIPNDRPFHEGDIVGIDSGINLNGY
                     FSDAAITLPVGSIAQETQKLLKVTRESLELAIEQVKPHARVSDISRAVFSHATKNGFK
                     LVRQYCGHGVGLEIHEDPQIPNYVSPGPNPRLMPGMVIAIEPMINIGTSDVRVLDDDW
                     TVVTMDGSLSAHFEHTVLVTDSGHEVLTKW"
     gene            4121..5632
                     /locus_tag="PLS88_01720"
     CDS             4121..5632
                     /locus_tag="PLS88_01720"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOU06105.1"
                     /translation="MSLRKKLYSRDFFVANLVILGIVLGFGLAFLFRAPTSESSSLPV
                     VKAESPSMISSPDIEASIETAESVQNALRNIAKTVLPSVVELDVVEGVQQTQQQTPSF
                     PFYFFFGPDSPFPDQFQPQEGLGSGVIVRRTGKTVYVLTNHHVAGNATKITVKLSDGR
                     EYEGKLVGSDERKDIALVKFETDDSDIAIAKLGDSSKLQVGDLAIAVGSPFGYIASVT
                     AGIISALGRTGGPEENINDFIQTDAAINKGNSGGALVNINGEVIGINTWIASPSGGSV
                     GLGFSIPINNVKDAIDDLIVNGTVKYGWLGVSLSNISQDKASAKELGLEGKKGAFISH
                     VFINGPADKAGILPGDFITAVNGKTIQSTDELVRVVGDIKPGSTVKLEFIRSGVVMTA
                     NTKIELRDNAIASNNANIFPGVTVISLKSDSVDQKKIPSGVRGALVVDVLAKSPAASM
                     GLQSGDIITKINNKNISDLKDFYELLAKESEKKLVFTVNRNGQTIETLAYVRK"
     gene            5741..6358
                     /locus_tag="PLS88_01725"
     CDS             5741..6358
                     /locus_tag="PLS88_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOU06106.1"
                     /translation="MHKEFVTYDTVRNNAIKLAYKIYRDGFIPDIIYVSLRGGAYMGN
                     VISEYFKFVKDDGRPVFYAAVVARSYTGFDQQESIKIDGWTYNPDFLRHGDKVLLVDD
                     VFDSGRTINYLARVIMERGLPRSDVKIAVHDYKVKDYIPQRLPILPDYWCRKIEVKSK
                     EDELWIHYMSHELEGLTQQEIVEHYLKDDPSLAEPMSFVAKKSAY"
     gene            complement(6385..7659)
                     /locus_tag="PLS88_01730"
     CDS             complement(6385..7659)
                     /locus_tag="PLS88_01730"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOU06107.1"
                     /translation="MAEILSIVLGGGKGTRLFPLTKERAKPAVPFGGKYRIVDIPLSN
                     CINSGFRKIYILTQFNSASLHLHIYRSYNFDRFSKGFVEILAAEQTHSHSGWYEGTAD
                     AVRKNLIHFRPHNPTHYLIVSGDQLYRMDLAEMFKEHIESGSRLTVAGTLVDRETASY
                     LGIIKIKANSSIDSFLEKPGPTKDIHEYEVPKDLKPDTANPSKPYLASMGIYIFDASL
                     IEETMQLDANDFGKEIIPFVVSKEKVNAYVFDGYWEDIGTVRNFYEANIELTDINPKF
                     NFYDEQSPIYTHARNLPASKLNYCTLNQALASDGCIITNASITRSIVGIRTVIESGAS
                     LDGVVCMGSDYYESAAEKDDNRRKNIPDIGIGGGSIIRQAIIDKNARIGSNCRIGIDT
                     LVRQEGEYSTHYIVDGIIVIPKNQIVPDGTVI"
     gene            7723..10038
                     /locus_tag="PLS88_01735"
     CDS             7723..10038
                     /locus_tag="PLS88_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU06108.1"
                     /translation="MNSESANAIYGEFVSWLAESSEIAWSSRIPAKSADCMPFPDDLS
                     PILSEALHKRGIDSLYTHQRKAYDLVRSGHDIVIVTPTASGKTFCYNLPILQTLLEMP
                     NARALYLFPTKALSQDQQSGLNEVMLAGSIGLPINTYDGDTPSDIRSKARTDGRIIIS
                     NPDMLHAGILPNHTKWVRFFSNLKFIVVDELHAYRGVFGSHVASVIRRLLRIAKFYGS
                     SPSFIFSSATIGNPQELAEHYIERKVEIIEENGAGSGEKILVCINPSLIDEVQGLRRS
                     NALEAESVMLWLLQKGIRTILFSRSRLQVELLAHYLNQKLENPYNRNYGIVVKPYRSG
                     LLPSERREIERGLRNGTVHGVVSTNALELGIDIGGLDAAVITGYPGSIASFWQQAGRA
                     GRTSGLSLAVYVASSSPLDQYFVSHPEYFLSRGAEQAHIDAYNPYIFTDHLKCAAFEL
                     PFLDDEAFSPDNSNRKAAELTSEALEYLEEEGIVRHVEGRYFWSGEGYPGEKISLRSA
                     TTENVVIVDISNGHHDVIGEMDRPSAKELLFEKAIYIHLGTQYQVKTLDFEKRICLVE
                     SSDADYWTDSIVKRDIELLTEDSTESHSEFRLILGDILVRSQVEKYKKLRFNTHENIG
                     YGEIWQEPEEMQTRSLMMVVDPQSQSGKFLSQFGPELANSIVYGIANLIRQLAPAKIL
                     CDIHDIGVASRAIDPFYKSPVIYFYDMYPGGTGIAEALCSNMSGIIRSTIERIASCPC
                     LGGCPSCIGVENANPDTKAASLKLLNLLSCS"
     gene            10100..11362
                     /locus_tag="PLS88_01740"
     CDS             10100..11362
                     /locus_tag="PLS88_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOU06109.1"
                     /translation="MNLKERLALIKSEKTKKNTSASRERNMPENWHNILPYVWTREIQ
                     GPIFIVPSFFYLSIPRSLHSSEIEESIDSQAPAFQIAADKIVFFDLETTGLSGGAGTI
                     AFLSTTARFEGRNLILNQVFLEDYPGERDFLSVVISQLSAGDCIASYNGAAFDIPLLQ
                     SRCILNGIIMPARLHIDVLQDCRRFWGKILDSCSLSSMERFILKITREFDIPGAAIPK
                     VWLDYVKSDSLNENQKALMEMVWQHNIMDVVSLAKLFFHTESAYRDPYRAVIEDAVDP
                     LGLVHRLCKLGRLEDAKSLLYMIYKNHEDNDLSTEIMRRVHQYLAKLARKDKDWELFS
                     EIVLSMDEEFLYGCVAKAKLFEHRFKDDDTALIWARKAHDLACNFGYSGAIERKDKDM
                     AAQLSVIASIDHRIARLERKIAKRKGIS"
     gene            complement(11288..12388)
                     /gene="rlmN"
                     /locus_tag="PLS88_01745"
     CDS             complement(11288..12388)
                     /gene="rlmN"
                     /locus_tag="PLS88_01745"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOU06110.1"
                     /translation="MNYNSAEELNIVYPSGLSQEELVAILSEEPLYRAKQAFLWLSRG
                     VRSFEEMTNLPVALRERLAEHFNGQVRSSRVENEFSDKDGSLKLQIRLRDGAAVECVL
                     LEDIEGRKTACLSSQVGCAMGCAFCKTGTLGFLRNLGPDEIVEQFHYLRERCGFISNV
                     VFMGMGEPLLNLDNVRKSIYILSNPAGIGLSMRKITISTSGIVPGIIDLAKNGPIVRL
                     AVSLTSADPYIRTELMPVNRKWPLDVLKPALLEYQKSTNDRITFEVVLIGGLNVREKD
                     AIELANWITPIKSQVNLIPWNPINGMPFKTPTVRQIEKFSQILLERGIVTVRRMSRGR
                     GVMGACGQLGDTLSFCDFTLKPSNTMINRGND"
     gene            complement(12385..13521)
                     /locus_tag="PLS88_01750"
     CDS             complement(12385..13521)
                     /locus_tag="PLS88_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU06111.1"
                     /translation="MPSLPYNDDFFEIPNEIQLENTEVAEISKKGYQLLKENRFDEAV
                     ECFIQILEKDKENNYALVGLGDAARKQERFKEAADYYRRCLVFHPGNSYALFGLADCY
                     KALNQFQKAIEIWEQYLLHDNTNITVLTRVADAYRKVRDFRKSKSIYHRVLELNPDNH
                     YALIGLGHLHYDFKEYKEALSYWQRMVELEGDNVDIRVLTAIGNCYRKLKHFDKGIPI
                     FEKALEKEPDNFYALFGIADCYRGVGKQNMSLVYWNKILEKDPKNKVILTRAGDAYRN
                     MGDFEKATEYYQNALNIEFDIYAVLGLAVIARQQGKYDEAIASLRSLLQNDPKNYRIY
                     VELAQTYLMNNQRQMAVEILTEFQQMGIKNPVIQDALFKLTAKT"
     gene            complement(13697..14233)
                     /gene="rsmD"
                     /locus_tag="PLS88_01755"
     CDS             complement(13697..14233)
                     /gene="rsmD"
                     /locus_tag="PLS88_01755"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOU06112.1"
                     /translation="MRITGGEYSGRIVKMPEGDLRIRPSMDRMRQSVFDVLGDLQGYS
                     FLDLFAGSGILGLEAASRGANPVVCIEKDRKKFPVLLQNASIAQPAIFCKAMPAETFI
                     LRNKQAFDIIFLDPPFDYKFKLQLLERLDRSASIHNGSLVLIHFPHEDKLPEQISRLK
                     CYDIRIFGRSIVHFYRRI"
     gene            complement(14239..14706)
                     /gene="smpB"
                     /locus_tag="PLS88_01760"
     CDS             complement(14239..14706)
                     /gene="smpB"
                     /locus_tag="PLS88_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOU06113.1"
                     /translation="MKTQDSIKTIAINRRARFDYDIEERLECGIVLFGSEVKSIKEGR
                     VSFSDAFAEIKGQEVWLNNLHIADYPHSSIFRHDPDRPKKLLLHRQEIKRLDRRVREK
                     GFTLIPLSIYLKHGLIKLELGLCRGKKAYDKRAEIKARDLDREIRREFKVKDW"
     gene            complement(14703..16256)
                     /locus_tag="PLS88_01765"
     CDS             complement(14703..16256)
                     /locus_tag="PLS88_01765"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU06114.1"
                     /translation="MKESEKITDLDIEKVKITLKPIFGIRPRIYIPIIYALIILIILF
                     FLLINPGLSNPGAKLSFEGNPNIAALYIENKYIGNTSDGIKLRPGSYQIQIRKRGFEP
                     KTIDIKVPNRIFATLILPPRVRIQYELEAESLESILIPSFREFSSWSLSGRPSAIYQL
                     PMVLSEASKDLVALPPADRVSIAKPLLAAGLSAAQNSSSIRDVIFASFSLGAPSASPL
                     GFVSMARNVTALLASSKNYVAAFSEIAPERTDDTIRGALSTLEKETSTFETTLYNSIG
                     VHMVGPHSFIMFNGGNLKGLTITPGGTRVPYIAYVPEFGLSSTEVTQRQFAGFLKENP
                     EWMPENRATLIGKGFVDSSYLADFDISSPSDKPITGVSWYAANAYCAWLSKNAPLGYK
                     VELPTEAMWEMAATQASRKIADLGVFSNRSSSGPISVGYSGHDASGFSDLFGNVWEWT
                     SDSFRPYKWIVEDSPAFDILANSGDSKTVKGGSWANNADQISIESRGPMPADHSSEFL
                     GFRPALIRK"
     gene            16326..17300
                     /gene="lepB"
                     /locus_tag="PLS88_01770"
     CDS             16326..17300
                     /gene="lepB"
                     /locus_tag="PLS88_01770"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOU06115.1"
                     /translation="MKNMDICDRLVVFSEDLLTRRKQRSLRAKMKQQKRHPILDWIYA
                     ILWAACVVLVINQYFFQNYRIPSGSMEKTLLVGDMIFVDKLSYGPELLPGVLKTPGIS
                     KPKRGNVIVFENPTYLSRGPIYTIFQQLLYMVTFTLVDIDREATGEPRVHYLIKRAIG
                     VGGDTLRVRNGEVSIKPIGSSEFLDERMLMEGLGLPVKMQRLVNSSEYSEIDNVGIAS
                     AYAELDLPLPSRVGMPSVQNANKDAFQYDMIRVTTLRDADPSNSRNAQLAQRYKNGWY
                     IADSRIFPMGDNRDNSRDARYFGPIAEKKVLGHALFIYFPFSRIGSIH"
     gene            17313..17894
                     /locus_tag="PLS88_01775"
     CDS             17313..17894
                     /locus_tag="PLS88_01775"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOU06116.1"
                     /translation="MFAKRPIRYAEKIERKKARNRILIYITVFIILCIIIRTFIFQTW
                     KIRGDLMKPTLRDGDIVIVVPCLLFAEEKSIGIAGLPKKGDVVLVGDGSEFILPSIAM
                     IKDSVLRFITLQKYSLLATEFGKTFSLPAVMRIQGIQQEKNNLKTWTVFKLATDSQGD
                     NFSMTGSQIVLPSRIKGRVVLRIWPLARIGKVQ"
     gene            17891..19156
                     /gene="hemW"
                     /locus_tag="PLS88_01780"
     CDS             17891..19156
                     /gene="hemW"
                     /locus_tag="PLS88_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOU06117.1"
                     /translation="MKPMALHIHEHEGITFSNIDPFEIHSLYVHIPFCTDRCSYCDFF
                     SVPFANNSHICSLDYQISYVQAILLNFQNWSKLFRAGSFSTIYLGGGTPSVLDKQVLK
                     KLIDRLEPHADRYCEWTIEANPESLDKALLDMLACTKVNRISLGIQTLSEEEWPILRR
                     VGSIDDAIQAIELLCEYPFEISVDLLLGIPHIPHKGDKRHKGKSYILQSLEYLVSRVS
                     HISIYDLTLEKGTLIESQVSSGEFAMLDEDELAEERRRIDIYLESQGFHRYEVSNYAK
                     LGHECRHNAAYWKMEPYLGVGCGGVSTIQYPACENSQELGTILRITETKDINSYIQSP
                     TEIPKDIEIIDRKTALFEFLMMGFRTSRGIDKKRITSLFGINLEECIPFTLKRWNSEI
                     IRSGDLIALKPYNFDILNRFLIECLEELG"
     gene            19168..20427
                     /locus_tag="PLS88_01785"
     CDS             19168..20427
                     /locus_tag="PLS88_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06118.1"
                     /translation="MAIFQFVVVSSILHEEPQKMRLLSAFEDNLQRAGGELLSLGSTL
                     INPRNSALPLFILVLTGGTESEAMRLISKERMLELKMPIVLLAHPSQNSLSASLEILA
                     KIRQDGGKGLILQADTSGAFDAEELEDIIAVCQSKIAMRNCRIGVVGKPSDWLIASSQ
                     DSLVALNSWGVKLINIPLEELLQNIGEIRSNSLETPIIEDLQEKAEFFAESNDTDLLK
                     SIEILQALRILVDRYRLSSLTLRCFDLVLKDKSTGCLALSQLSDDGIDAGCEGDIPSI
                     IGLSWIRSLTGQIGWMANPSNIIAKKEDDLAEMLIAHCTAPRSILSKYALRSHFESGL
                     GVAIAGIFPKEEVTLFRIGGKYLEKLWYSPAQVIESPRSEGLCRTQVLLNLPNKKAQE
                     LLTNPLGNHIVMVRGNWTKRISIFEQL"
     gene            complement(20443..21051)
                     /locus_tag="PLS88_01790"
     CDS             complement(20443..21051)
                     /locus_tag="PLS88_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU06119.1"
                     /translation="MKIYHHYSVYGFSNIYIIVNESLKKAIIVDPAELTPTMIERIEQ
                     NKLDLCGILITHNHIHHVKGLSTIMKIYSPTIYASNAKLLGFQCRKLRDGDVFSEAGF
                     EITAIAVPGHSQDSIVYFMEGNFLFTGDVLHAGMIGKTNSVFNTNALIARIKQKLFCL
                     PESTLVLPGHGPPSTIGTEKSANISFQEGFAERIRPNYDFFV"
     gene            complement(21048..21647)
                     /locus_tag="PLS88_01795"
     CDS             complement(21048..21647)
                     /locus_tag="PLS88_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOU06120.1"
                     /translation="MYKVSSCAIEGCSLPAFSIENLCYHHLQDYPERMKRAMQELQSN
                     NTILNRVFDYAFLMGLDFSRYRFVGCSFRNARIQHSLFTGASFQLCFLDSSSFFSCDL
                     SGSDVDFCSFGKCSFMDCSFENSELIQSSFNGSVIVDCTFAGSNLYNSRFIMCDMNTV
                     NIDNCDFKRAFYIPSKEQKVSFSRSNIAEAVRDLEHLYL"
     gene            22097..22897
                     /locus_tag="PLS88_01800"
     CDS             22097..22897
                     /locus_tag="PLS88_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOU06121.1"
                     /translation="MRICVFDSGIGGLPFVQALNREFPFLEIDYVADDAGFPYGTKSP
                     EMIRDMLFERLRRIRARLDPEILIISCMTAVQIGLKELQAAHRALNIIGAIPPIAEAA
                     KESQTHRIALLSTSRTAEDSFLDYLIARDAPDSEVLRIPAQDLIEYAEQHLPFASLLS
                     AQTMVAPYINYALEEGADRIVLASSQFILLEDAIASQLNERQAFNVQCLDPRAKVISA
                     LQKLIHYKEHAEETKFQKPRFFLAGDRGATPYYIAWSDKFNCSTPQLL"
     gene            22894..23901
                     /gene="rsgA"
                     /locus_tag="PLS88_01805"
     CDS             22894..23901
                     /gene="rsgA"
                     /locus_tag="PLS88_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOU06122.1"
                     /translation="MNRNSNESMVLGVVISFSNRYAKVRCDDGNIRTCTIKGKRIDSS
                     SRGYKNLAAGDHVSVIPLSKQNGVIESIFDRKSEFGRYNEKGEARQVFAANLDLVVCI
                     ASTRAPPFRPRFIDRVGVMAEYCGLPFLIVCNKIDLGLDISIESRLAAFQELGYGVIK
                     SSVRDSIGIDELRKVLIGKTSIFIGQSGTGKSSIINCLIPGADQRIAEISEKYDRGKH
                     TTTLSTMLSSPNEDFNIIDTPGIRRLAIRNIEPNNLAYYFPDMVPFLGLCEFGASCTH
                     RYELHCFVKQAVQEGLINNDRYESYLRMRAELEETKNAKTNEARRLQRSATDQFEEEE
                     W"
     gene            23895..26357
                     /locus_tag="PLS88_01810"
     CDS             23895..26357
                     /locus_tag="PLS88_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOU06123.1"
                     /translation="MVTNEHSLALLDFYRLRNRLEEYCFSHEGRALMNSTLPCSHEDS
                     VKRLKKDLSLLVEGLSDKEMPSFPFPNIEIPITKALKEGTMLEVDELFAIGLWAKSYQ
                     QFFAFLRSLINPLVEVNHETWEEDFSNDEAWAKLGIENIVHSAPSLINAVQIVFAVIS
                     EDGEIKDLPRLKSLKLAIQKTQAENLHIINRYLSDPTLQDALQSNIATERDNRTVIAI
                     KANFKGRIKGIVHEFSSTGQTIFIEPLELVERNNKLLELDSKLNEEIRAILRETTEAL
                     RQYLPILIRGRGLLASADVRLARAIQLKREDLAIAGNLEQGIHLYQAKHPLLGKKAVP
                     IDVDIPDGARILIITGPNTGGKTVSLKTIGLLALLNQYGAGIPALSTSAFAVFDTILA
                     DIGDEQSIDQSLSTFSGHMNIISGIVSSATDRSLVLLDELGAGTDPEEGCAIAMALLD
                     YFHHLQCFAIVSTHHGVLKNYGYTKPGCLNASMEFDAESLSPNYRIIMGVPGESRALE
                     IASQIGLPKEIVDVAKAYLEDERSDYSVIIHSLGEKQKELEKLELEKKRQLKKVIDSR
                     REVDLENLRIRQRELELRKQGVAELKNFLTESRKIFENIVRELRENGIKDEDTSSGRE
                     FLRKMSDEIHQQSEELESFSEETQRIERLMKPDMQSSIPIDIDKIKSGDTVIFQDREA
                     IFLRMLDEKKALVQLGSIRIPIECEALSLPRSRNKTEIVSKLGYQIELASDSMAKRMP
                     VAELDVRGMRLSEALDALEHQIDAASLAGISNFAIIHGTGEGILSRGIHEWLSMQSSV
                     ADYYFARPEDGGFGKTWIHLKE"
     gene            26505..27239
                     /locus_tag="PLS88_01815"
     CDS             26505..27239
                     /locus_tag="PLS88_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU06124.1"
                     /translation="MSGHSKWASIKHKKGALDAKRGQLFTKLIKEISIAARMGGGDIE
                     GNPRLRTAVLKARAANMPKDNIDRAIKKGTGELEGSIYEELFYEAYTPGGGAMLIEVL
                     TDNKNRAAAEIRNIITRSGANLGTTGSVAYLFKRKGVLTYDSEKYTEDQIMEAALDAG
                     ADDVVSEDGTISVYTDPASFEDVLNMMNDKGFETLGAEISMLPDSYITVDLETAQKIQ
                     RLIDKLEENEDVQSVYHNIEMPELEE"
     gene            27248..27754
                     /gene="ruvC"
                     /locus_tag="PLS88_01820"
     CDS             27248..27754
                     /gene="ruvC"
                     /locus_tag="PLS88_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOU06125.1"
                     /translation="MNSQSSLLAIGIDPGIASIGYGLVRTFEGRLSLIAYGCIETESD
                     EPIGKRLTYIFEEIRKLLGQYAPDIGGIEELYFFRNVSSAFPVAEARGVIRLAFEKTG
                     VVLREYSPNAIKKAVTGSSRASKSQVQEMVKVLLGMSQIPTPDHAADALAAAICTLHS
                     IGPAGYTL"
     gene            27756..28361
                     /gene="ruvA"
                     /locus_tag="PLS88_01825"
     CDS             27756..28361
                     /gene="ruvA"
                     /locus_tag="PLS88_01825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOU06126.1"
                     /translation="MFNRIKGVFSGYADEALHISTGAIEWEIFVPIRDPSFYTHTGEE
                     IELFTWLHHYEDAMRLYGFFSAVERQLFLDLQKVEGIGPRQAFKILQGIAPRSLATML
                     DRGDLASLQKIPGIGPKTAQKMMLALKGKLITPEEASAAISNSQGRFRDVIRALVEMG
                     FERKEVERVTAQLASSLEDGPEAEKELFKRALLELSAGAGA"
     gene            28400..29404
                     /gene="ruvB"
                     /locus_tag="PLS88_01830"
     CDS             28400..29404
                     /gene="ruvB"
                     /locus_tag="PLS88_01830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOU06127.1"
                     /translation="MNPLYNPAEDEKEASLRPRYLSEFQGQQRIKDNLLIFIKAARDR
                     GEALDHLFLMGPPGLGKTTLALVVASEMGVDFKPTSAPAIEKPKDLAGILTNLRPAGV
                     LFIDEIHRLKPSIEEMLYIAMEDFELDWIIGQGPAARTVRIPIPPFTLVGATTKAGMV
                     SAPLSSRFGISLRFDYYSPEELASVIRRSSNILDIKIKEDAIELLSGTSRGTPRIANR
                     LLRRMRDFAQVRGEGIITRDIVEDGLERLEVDYLGLERLDREILKTIISKFGGGPVGA
                     ETLAVSVSESVESLEDYYEPYLIQIGMLARTPRGRVATPIAYRHLGMEKLDENGQFQF
                     "
     gene            29379..30488
                     /gene="queA"
                     /locus_tag="PLS88_01835"
     CDS             29379..30488
                     /gene="queA"
                     /locus_tag="PLS88_01835"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOU06128.1"
                     /translation="MKTDSFNFDLPKHLIAQYPTEERGRSRLMVLDRRTGNIVDSMVE
                     DVASFIEPNALMVFNDSKVRKARLYGRCEQTGTSVEFIFLSPWKQDDLISLRGSEFNH
                     EGQENLVTACEQVSSVSRLWRAVCNKSKRQNIGRSYIFPGQLRGSVVEDRGDEKIIEF
                     SSNIGEEYFDLYGHVPLPPYIKREDTGNDEVRYQTVFAKHTGSAASPTAGLHFTPQLL
                     DELSQKGIEIEYLTLHIGLGTFQPVRVENIEDHHMHEEWFEIPFQVAERINLALREGR
                     KVLAVGTTSMRALESAWNRGQVRHGNFSTRIFIYPGYEFHVVNSLFTNFHTPKSTLIM
                     LVSAFAGREKIMQSYQLAIDKGYRFFSYGDAMLIL"
     gene            complement(30559..31200)
                     /gene="tmk"
                     /locus_tag="PLS88_01840"
     CDS             complement(30559..31200)
                     /gene="tmk"
                     /locus_tag="PLS88_01840"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOU06129.1"
                     /translation="MPSYNTDEFKNRFIVIEGIDGAGTTTQVRLLAESLIAGGFSCIV
                     TAEPTMNHIGQLIRTILSGEDIVAPTTVAHLFAADRNEHLYGKNGIIESLKAGSMVVS
                     DRYVLSSLAYQGTTCGPRLPWLLNSTFPAPGLTLLFEVDPEICITRIRSRLKKEIYET
                     LEFQRKVQNMYERMADRLSHKGWRIERIDGSKDIESVHQQIESIVLSFLGVEC"
     gene            31254..37187
                     /locus_tag="PLS88_01845"
     CDS             31254..37187
                     /locus_tag="PLS88_01845"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOU06130.1"
                     /translation="MKTKKSVILIVLFSLTLAICGSIAIIDLVQREVQVLAIRALDKL
                     QSTIVDTTGLILKYDYLDIESLNRFSMHGIELIRSDPLDQNNSEFPSYLEFSLPSERS
                     NYKEQPPLKINKLSVRISFWALFAGQNNEAVRDITVDDVQISLYFPNDEYIINKILAN
                     FVSGKAKSLPHFELSIGPIRAEVREIAKQPNSSIDKDVINGGSLTIESLQFSSLTDSP
                     VILAPSVILKANGLFGLPKELNAEFSLRGSGNADLSNIDMNIELQASSTNWHIIPQQL
                     LLQKRGSRIECEREGGGGLVRGWYESGEWGLEGVFEGYRAGEDIGVGGVWGNLVRGSE
                     VSGKVEVGGKGKELVGYEVDLGIEIGEGIEIFGKGVGGIGVRLKGVGDIGRYIGEVSV
                     VKGGGKVRYEGEVGYEGIRLVGRYEVSGEGIGIRGEVRGGGGAYEIGVEGGGVKGIGV
                     GAGICKVKVGEESYEVRYEGVIGGGEVKLGGSIGKLGGYEGVVELEGVGIGKVMEAIG
                     VRGVEGKVRGRIYGLERGGKISWSISGLGYEGEVMGIGVGIRGEGVGDEKGYEIKGLE
                     VSIGSYDIGLRGKGIYGGELFSGEVSGGGMRYEVRVGIRGGEVRVEVGKECSGVFEVS
                     GGRVGGEVGMDNFGIGIGDGVMRLGGKARGWYEGGHWGISLDGISLRYEGEGVYPELR
                     VSGSAGEGGLRFEQLEVIGEGYGFKGSIDLKEEGGRYIFIGSGRGEGALGEGSEYSIW
                     GGVKGKGVELRVGVQGLEVRGYEVEGYADLIGEVDIGELLKGSWEGLGDISGEVAIGL
                     RGSGLSVVEQRFKVSKRGSRIECEREGGGGLVRGWYESGEWGLEGVFEGYRAGEDIGV
                     GGVWGNLVRGSEVSGKVEVGGKGKELVGYEVDLGIEIGEGIEIFGKGVGGIGVRLKGV
                     GDIGRYIGEVSVVKGGGKVRYEGEVGYEGIRLVGRYEVSGEGIGIRGEVRGGGGAYEI
                     GVEGGGVKGIGVGAGICKVKVGEESYEVRYEGVIGGGEVKLGGSIGKLGGYEGVVELE
                     GVGIGKVMEAIGVRGVEGKVRGRIYGLERGGKISWSISGLGYEGEVMGIGVGIRGEGV
                     GDEKGYEIKGLEVSIGSYDIGLRGKGIYGGELFSGEVSGGGMRYEVRVGIRGGEVRVE
                     VGKECSGVFEVSGGRVGGEVGMDNFGIGIGDGVMRLGGKARGWYEGGHWGISLDGISL
                     RYEGEGVYPELRVSGSAGEQEANLNIDYLQYAGQSLKGSMAIQYSSIANLLKDTKINY
                     SFLSIETNRPILEGRIVALDNNLEVSIIASSIPLEEILPSTLNISGDIELKGSATILI
                     SGKKFNLSDISYLGLQFNTRKAEMKGISFSASAVIKLKGSALSLESGFFSYQNYRFEN
                     INAEYDIDTKQVTYDLNSKIVIGKQLLSARLKGIGENVDDSIFKPDMAQNFNIIGDII
                     EAKFGSRAIDPLTYSISYLEHNLQIHLSQANGDSIDASINNMTDFDLVLNDLFKVKGK
                     ASGKIQANEVSADIVLEKIDLTSLGALISKDILRDISGDASAVLHLSGDISDPRIEGR
                     ILFSNFIIDPNLYVLEKMGPFNAEITISDGVVELSPTIVNIGTGDISLAATANLARWQ
                     LSDIKVFFSTLEKASMRLKGTIGGLNTDGIWIKGDIKAAISSDFVEIGGNILLDNGTL
                     EVDPNGFVFSENTQSPVSPLYLNLIVTFGKNVQMYIPNKDISLIKGTISPTSTLKVQY
                     DQAKGDLAVNGSIELRSGYIYYLLRDFFIKECTIELSENQSKFNPVMTVIAELMEPSK
                     DGMIKITLSANKSPIDNFNPVLSSVPPKSQIELLALLGGGLALSELAENTPLTLKEAV
                     IASSEFLTHNLVFRSFEQRVQKALGLDVLYLQSSFIQRWLLDITDEAHKDQTNLSKYL
                     IGTGLFGGKYITDSAFAHFSLRIEQNPLEKTGFLQLNPEIGLELQSPFGLLKWSMSFG
                     KEGMPLNNQDLSLSWRINF"
     gene            37206..39758
                     /gene="bamA"
                     /locus_tag="PLS88_01850"
     CDS             37206..39758
                     /gene="bamA"
                     /locus_tag="PLS88_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOU06131.1"
                     /translation="MKYSNRLFISIIMIFCAFQIICAQTSTEELPHQDNSQDWFWNKK
                     IAGFKWDGLHYANRNDLDSMLRGYIGTFFTENIWIEIQAKLYALDWFESIESIALPAG
                     DEEDRIIIKFIVIEKPSISSIKVIGNSSIRTSELLDVASSKTGAIFNEDKAKADALAI
                     QKFYLQKGFPDAKVEYEKNPAAGNPNLIVLTFKVTEGTAVVVRKIAFSGNSAFSSQTL
                     KGQVTLKEAGLFQKGAYEESKLAESRTALEDYYASKGYVDAKVIDVIKNYSKDEKSQK
                     NYIDITFVIAEGKQWRLGDIVFEGNSIFSTSRLASLISLKQNEPINLKRLLTDKQKID
                     DLYYESGYIFNNITMTEVRDEQTGKISFKISIIERDRAHIESITFKGNTKTKDYVIAR
                     EIPLEAGEVFSKTKIIDGLRNLYSLQYFSSIEPEIHQGSVIDLMDLVINVEEMSTSQI
                     QFGLTLSGLGQQTAFPISGFVKFSDLNLGGTGKNLQVNMTLSPDEQSLETSFGQSWLF
                     NKRISQSLSFNIAHSILQTAQDSIAPIFSEEDIPDPFIALGSGSNEWDGLLSSVSDTY
                     LMDYDDYAFSLGYSMGYVHKISQGDVGISGGVSSGLDMSSYDSAKYRPYEKEMRDYNG
                     QWVLKNSIFGRVYLNKLDYWYNPGKGYFLSERLAYTGLLPIERQFYIRSDTRAELYAT
                     LFSIPFSSTWIFSPVLGIHSGVQALFPQPWNSLKVTKDWVYIDGTFNMRGWKSLYNGH
                     GILVWENWLELHLPLVPQILSLDGFFDAGAMMTENGWLDMTFDSPVSTNSDTLGWNNF
                     AFSVGFGARFIIPQFPFRFYLAKRFVYDGSKVEWKTKGGSFDFVLSITQPLY"
     gene            39838..42426
                     /gene="mutS"
                     /locus_tag="PLS88_01855"
     CDS             39838..42426
                     /gene="mutS"
                     /locus_tag="PLS88_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOU06132.1"
                     /translation="MSSKSDSMLDQYRRIKAKHRDEILFFRLGDFYEMFNEDAQEASA
                     ILDLTLTKRQGLPMCGIPYHAAKAYIARLLKAGKKVAICEQKALSNNKRGLMDREVVE
                     VITPGTALEDDYLEQGTNNYLADICIIDQSLCFAYLDVSTGEFRAFSLAFKREMVAET
                     IRAELYRLSPREILVQQSILDQPKLFQVIRECGSIIEAQPDWVYDISHSLDLLKKKFG
                     IVSLKGFGFEDNAPEIAAAGNLLEYVQEMIRKNCPHIRALLPYEEKEYVLLDEATKRN
                     LELVKNLSDSSKRDTLIEVIDKTKTAGGARLLRQWLFQPLRIREKIEERLEAISFLYH
                     NQILLTDIRKLLYGILDIERLISRLAVDKAHARDLRAIHDSINIGINLFALIASAQAP
                     FELTSRMSKEQLEACKHVIDTIEKTILEEPSILLTEGNIIRDGYDKELDDLRNLHKNT
                     QKVLEDYLKDEKKRTGIQNLRIRYNRIIGYYIEVTKGNLANVPSHFIRRQSLVGAERY
                     TTDRLAELESKINGAQERIIDIEKNIFIEIRDSLKQFIPALLECASEISRIDCLASLA
                     QTATEQGFTKPQIEEEEDIEIINGRHPVVEICLPYGDFVPNSISLSRKSNWFILITGP
                     NMAGKSTVLRQTALIVLLAHMGSFVPAESAKIGLVDKIYCRVGAQDNLARGESTFLVE
                     MHETAFILNTATEKSLVIMDEVGRGTGTLDGVSIAWAVSEYLIKRISCRTLFATHYHE
                     LTTMAIPGMRNMRMVATEQDGNISFPKRLEDGASSGSYGIHVANLAGLPKEVIIRASE
                     VESHFNSMEQALKEEKPEETAVPSYELFSAEDIVIETLKNIDPNNITPIKALQILSDL
                     VERLKQ"
     gene            42435..43172
                     /locus_tag="PLS88_01860"
     CDS             42435..43172
                     /locus_tag="PLS88_01860"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HOU06133.1"
                     /translation="MSDYLMMDSIFRKANAQTLDYIFPQHCVLCGSIVSAVSWSPAPL
                     CASCERTLEFIKGPRCKHCGRELISELEYCMDCRNLQTSLTHIIPIFSFRGNAASLIH
                     AFKTGQRISLARYFAYRISMLDELVGNISTSICLVPVPPRPEKLKLGQFDQVGTIAES
                     LIEYGYHYKKMLLRLSGSSQQKLLDRASRLKNASLSYTLLSEKKTMERIILLDDVCTT
                     GATLEACARLLMQNGNEVAGAIVLAAD"
BASE COUNT        13117 a         8013 c        10160 g        12333 t
ORIGIN      
        1 ttgtgattat ccaagatgaa gaatctgatt cgataggggg gatttttgca agcggtgctc
       61 agagcgcctt tggttctagg acatcgaata ttgtggtgcg gataacctat gtgcttggcg
      121 gtctattctt tgtcatcgcc ttttcccttg caatattgaa taaatcttca acagggaata
      181 tccagaaagt agtagaacaa gaatctaccc aaacagcgac tagcgaatgg tggaattccc
      241 agggcgatgg ggatcagtct gtccaaagtc agcttgcgcc acaggaccag acaacccaac
      301 cagaggggct taatacaccc gccactaaat agctcttttc ccgcttaatt caaaaggctg
      361 gaacccaaag gaaggcacgg aagcgtgtta ttctgtgctt ttattctatg aagaattagt
      421 gtgaaattgc gtattcaata cgtaactagc taattcatat ggatgttttc taattattat
      481 gcgaattgga ataaatgcta ttggatcaag aagactttct tcagtttcta atgcaatgct
      541 gcattcattg aaagagaaag gtcatgattc aattctgaca tcagaatatt cctatagcct
      601 tggatccttt gatttcctta tctttctaac ggaaccaaag ggtatgtttg gtggtctctt
      661 gtcctcagtt gctcaggatc tggcaaaatc tggagatcta gtggggaaac gttgtcttgc
      721 tatggtgcaa aaaagaggat tcagatcaag tcatacactt aagaaattta tggaagcact
      781 agagcacgag ggcctagtga tcgtacaagg cgaagtcttt tccaatatcg atactgcgat
      841 tcatataatc gcggaggttc ctctgaaacg cggatgaaca atgaaaaatt attatgagat
      901 tcttggtgta gaacaagatt cgagccaaaa ggatataaaa agcgcattta gaagacaagc
      961 aaagcgtctc catccagata tgtattattc tacagggaaa gccagatctg aggagtcacc
     1021 tgcaacgctt cgcgaatcag ccatgaggct tatcctcgag gcatataaga tattatctga
     1081 tgctgaaaaa cgaagaatct acgatagaga gcttcaaagg aaagagaaag agaacaaagg
     1141 atttgattat cgcgaattcc ttaaaatgcg agcagacgat cctcaaagcc aagcaaaact
     1201 gatagttttt gatcttctgc atggtttcga ggaagaagca atatggatct atgaaaggag
     1261 taagagcttt caagatttcc ggcttgagcg ttggctcgag cgtggagaag ccatggattg
     1321 cgagtactgc atcgcagagg aatatgaaaa gcgaggcaaa tacatcaagg cttaccagat
     1381 ctacaagaag gttatacaga tggagatgga aaagccctgg tttaggtact actttgatgt
     1441 tgtcgctctt caatttaggt tgcttgtttt gcagaaattg cctggaaaga tagacgaaga
     1501 cgattatcta gataggctag atgaagcgat tgaattagga atttcgccgc gagagactgc
     1561 gcaatatctt agaaaaaaag tcgaaatctt agtgcataga ggagaagcag acagagccgc
     1621 agaaactctc atgcaaatat cgcagatcta tcccaagctt gctggctttg atgcattgca
     1681 cgcaaaagtt cagcgtgcac ttgagcagca ggttgcacaa gatagggtat actgaccagc
     1741 accgtatcgt gcttgttaat aaataatatc gagagcgagc ataatgttat gaagagaata
     1801 gcaacttgtt ttttagtctt tctgtttaca gcaatttcag cctttgcaca gaccagcata
     1861 gataaaccag cagcaacaat aaaacttgta aagcaagagg ttatttcagt tcgccagcta
     1921 cgaactgatg ttgaaaagct agagaattca gtaggtgtaa agctgacatt agatcaaaga
     1981 aaggatgttc tcgatgcgag aataaatagt atgctgttct tgcagttttg cgagcgcgaa
     2041 aaaatctcgg tatcggaagc tcaggtaaat gccgcgctat cacagctcag atctcagtta
     2101 ggtacaggcg ccacagatgc tgacctagag aaatcacttc gtgcttctgg cgtatttgta
     2161 gatcctgcta cttttgttcg tcaacggttg cttttcgaga cctatattca aaccaagaga
     2221 caagatgagc taaaggttgc gctcaaggca ccgactgcag atgaaatctt gaaagcctat
     2281 gatctggcaa aggcttcgct tgtgagacct gatacaattc gaatcagtgt gatttatgtg
     2341 gatactaaag gtaaaaccaa tgccgagatt gcaaaagcaa aagagcttat aaactcaatc
     2401 tcggtctctc tcaaatcaaa tcctacaaaa ttcgatgaat atgtgcttcg cgctagcgat
     2461 caagctggat acaaggcaat tccatcaatg tatctagaaa agactcaaca gagcaaatcg
     2521 atatttggcg atgagttttt caatgcggca tttcaagcaa atgtgggcga aattactccc
     2581 gttatcgaaa cgccagtggg ctacaggatt gtccgtgtca acgaatttct agctcaaaag
     2641 cagctgacac tctcagatcc agtcccagga aatcaaaatt tgacagtgca ggagttcctt
     2701 gctattcagc ttgcatccga gaaacaacaa gcctttttga acaaggctga agcggatctc
     2761 atcgaagcac tgcgtaaaga agcaactatc aagatttatt cggaaaactt aaattggtaa
     2821 gggacgattc aggtggcttc tagaagaaag gcaagaattc ttgcagtaca ggctatatat
     2881 gcttgggata tgtctcatca agaacttgag gatatattat ctttcgagtg gctagatgag
     2941 aataagaaag cacgctatga aatcgagatt ctagattttg ctaaattgct gataatcggg
     3001 actattgaac agatcgattc tatcgatgat ttaataaaaa aacatcttga acactggacc
     3061 ttcgaaagac ttcgaaaagt tgatttggca attctaaggg ttggaacata cagcctggta
     3121 tttcaacagg atattccagc acagatcagc atcgacgaag cgattgaaat tgcaaaagaa
     3181 tacggcagtg aagaatctta ccgttttatt aatggtgtgc tagatggcat aagaaaggac
     3241 tttttcggat aaccatatga ttcgaatcaa aaatgaaagg caaatcgagg gaattcgatc
     3301 atcctgtgat ttgctctctt tactctatga gaagctaaag cttcaagtgc agcccggtaa
     3361 tacgcccaag gatttggaca gatttgccta cgattttata ataaaacatg gaggaaagcc
     3421 cgcttttctt ggatatgagg gctatcctgc tactctctgt atttccgtaa atgaggtagt
     3481 catacacggc atacctaacg ataggccttt ccatgaaggc gatatagttg gcatagatag
     3541 tggtatcaac ctaaatggtt atttctctga tgcagctatc acattgcctg ttggatccat
     3601 agctcaggaa acgcaaaaat tgctaaaagt aacgagagaa agccttgagc ttgctataga
     3661 acaagtaaaa ccgcatgcgc gtgtttcgga tatctctagg gctgtttttt cccatgcaac
     3721 caagaatggg ttcaaactgg tgcgacaata ttgcggtcat ggagttggac tagaaatcca
     3781 cgaagaccct caaattccaa actatgtctc gccaggcccc aatccgaggc taatgccagg
     3841 aatggtcata gcaatcgagc ccatgatcaa tataggcaca agcgatgtac gtgtgctcga
     3901 cgacgattgg acagttgtaa cgatggatgg ttcgctttca gctcactttg agcatactgt
     3961 tctcgtaact gattcaggac acgaagttct aacgaaatgg taatattttt tcatttcttt
     4021 ttctaatttt ttatctattt tagagttcat ggaagagttt aattttatta tattggatac
     4081 agtttgcaat tactctgtca aataacggag gatatcgacc atgtctttaa gaaagaaatt
     4141 atattcacgg gattttttcg tcgcgaatct tgttattctt ggaatagtat taggattcgg
     4201 ccttgcattt ctatttaggg ctccaacatc agagagctct tctcttccag tagtcaaagc
     4261 cgagagtcca tccatgataa gcagtcccga cattgaagct tcaatagaga ctgcagaatc
     4321 agtgcagaat gcgctgcgca atattgcaaa gactgtactc ccttcggtcg tagagctcga
     4381 tgtagttgag ggtgtccagc agactcaaca acaaacgcca tcatttccat tttatttctt
     4441 ctttgggcct gacagtcctt ttccagatca attccagccc caagaaggtc tcggttctgg
     4501 cgtaatagtg cggaggactg ggaagacagt ctatgtcttg accaatcatc atgttgcagg
     4561 gaatgctaca aagattaccg taaagttgag cgatggccgt gaatacgaag gcaagctcgt
     4621 tggatcagat gagcggaaag atatagctct tgtaaaattc gagacagatg attctgacat
     4681 cgcgattgca aagcttggcg attcatctaa attacaagtt ggagatttgg caatcgcggt
     4741 tgggagtcca ttcggttata tcgcctcagt taccgctggc attatcagcg cattggggag
     4801 aaccggaggt ccggaagaaa atatcaatga ttttattcaa accgatgcag caattaacaa
     4861 aggtaattcc ggaggagctc tagtaaacat caacggagaa gttataggca taaatacatg
     4921 gattgcatcc cctagcggag gatcagtggg cctaggtttt tctattccta taaataatgt
     4981 aaaagatgcg atcgatgacc ttatagtaaa tggaactgtc aaatatggct ggttaggtgt
     5041 aagtctctcc aatatctctc aagacaaggc aagtgctaaa gaacttggtc tcgaaggcaa
     5101 gaaaggtgct tttatcagcc atgttttcat caatggtcca gcagataagg ctggaatctt
     5161 gcctggagat ttcattacag ccgtcaacgg aaagacgatt cagagcacag atgaactggt
     5221 aagggtagtg ggagacatca aacctggttc tacggttaaa ttggaattca ttcgaagcgg
     5281 agttgtgatg actgcgaaca caaaaattga gcttagagat aatgcgattg cgtcgaacaa
     5341 tgccaatata ttcccaggtg tgactgttat ttcgctcaaa tcggactctg tcgatcagaa
     5401 aaaaataccc agcggagtac gtggagctct tgttgtcgat gttcttgcaa aatcgcctgc
     5461 ggcatcaatg ggcctgcaga gcggagacat tatcactaaa atcaataaca agaacatcag
     5521 cgatcttaaa gatttctacg aactgctagc taaagaatcc gagaaaaagc tagttttcac
     5581 cgtcaatcga aatgggcaaa caatcgagac ccttgcttat gtaaggaagt aagtatatat
     5641 tccttttcta gggaaagtca ttcgataagt gaagcctcct tatggaggct tcacttgttt
     5701 tttcaaggag gcttcacttg ttttttcaag aagggattct atgcataagg aattcgtaac
     5761 ttacgataca gtccgaaata atgctatcaa gcttgcctat aagatttata gagatggctt
     5821 tattccagat ataatctatg tttctcttag gggtggagcc tacatgggca atgtgataag
     5881 cgaatatttc aagtttgtaa aagatgatgg gcgtcctgtt ttttatgctg cagttgtagc
     5941 aaggtcatat actggttttg atcaacaaga atcaatcaag atcgatggat ggacctataa
     6001 tcccgatttc ctgcggcatg gcgataaagt gttgctcgtc gacgatgttt tcgactcggg
     6061 tcgaaccata aactatctag ctcgagttat aatggagcga ggcttgccga gaagcgatgt
     6121 aaagatagcg gttcatgact ataaggtaaa agattatata cctcagcgct tgcctatcct
     6181 tccagattat tggtgtagaa agattgaagt gaagtccaag gaggatgagc tttggattca
     6241 ttatatgagc catgaactag aaggtcttac tcagcaagaa atagtagagc actatctcaa
     6301 agatgaccct agcctagcag agcctatgag ttttgtggcc aaaaagagcg cttattgaat
     6361 gagcgttaat tgagctaaca ataattaaat tactgtacca tcaggaacta tctgattctt
     6421 tggaatgacg ataattccgt ccactatata atgagtgcta tattcgcctt cttggcgcac
     6481 aagagtatcg attccaatgc ggcagttcga gccaatacga gcattcttat ctattattgc
     6541 ttggcgtatg atagagccac ctccgatacc aatatcggga atattctttc tgcgattatc
     6601 atctttttct gctgcacttt cgtagtagtc tgaacccata cacacaaccc catcaaggct
     6661 agctcctgat tcgataacag tgcgaatacc tacaattgat cgagtaatcg acgcattagt
     6721 tattatgcaa ccatcgctgg ccagagcttg attcaatgtg caatagttga gtttagaagc
     6781 tggcaagttg cgtgcatgcg tatatattgg cgattgttcg tcataaaaat taaattttgg
     6841 gtttatgtct gtgagctcga tattggcttc atagaagtta cgaactgttc caatatcctc
     6901 ccagtaacca tcgaagacat atgcatttac cttttccttt gaaaccacaa atggaataat
     6961 ctccttgcca aaatcattcg cgtccagttg catggtttcc tctattaggg aggcatcgaa
     7021 tatatatata cccatgcttg ctagatatgg tttactggga ttggccgtgt ctggttttag
     7081 gtcttttggc acctcatatt catggatgtc cttagtagga ccaggctttt ccaagaaact
     7141 atcgatactg ctatttgctt ttatcttaat aattccaaga tagctggcag tttctcgatc
     7201 aaccaaggtc cctgcaaccg taaggcgact gccagactcg atatgttctt tgaacatctc
     7261 ggctagatcc atacgataaa gctgatcacc tgagacaatc aaataatgag tcggattgtg
     7321 tggtcggaag tgaataagat tctttctgac cgcatccgca gttccttcat accatccaga
     7381 atgcgaatga gtctgttccg cagctagaat ttcaacaaag cccttagaga aacggtcaaa
     7441 attgtaggaa cgatagatgt gaaggtgaag ggaagctgag ttgaattgtg tgagaatata
     7501 gatcttccta aagcccgaat tgatacagtt tgagaggggt atgtcgacaa ttcggtattt
     7561 cccaccaaac ggaacggcag gcttggctct ctcctttgtg aggggaaata gacgagttcc
     7621 cttgccgccc cctaacacga tagaaagaat ttctgccata tatgctcctt tcttatcagc
     7681 tgctgattta ccttttttgt tttgatgact acagtataac ttatgaattc tgaatccgca
     7741 aatgcaatct atggtgaatt cgtatcctgg ttggcagaaa gcagcgagat tgcttggtca
     7801 tccagaatac cagcgaaaag tgctgattgc atgccattcc cagatgattt atcgccaata
     7861 ctttctgaag cgctgcataa aaggggtata gattctctct acacacatca acggaaggcc
     7921 tatgatttag ttcgtagtgg acatgatata gtaattgtaa caccgacagc ctctggaaag
     7981 acattctgct acaatcttcc catccttcag actctccttg agatgccgaa tgctagagcg
     8041 ctctacctat ttcccacaaa agctctatct caagatcagc aaagtgggct caatgaggtt
     8101 atgctagctg gaagtatcgg tttgcctatt aatacttacg atggggatac tccatcggat
     8161 attcgctcca aagcacgaac agatggtcgc ataatcattt ccaacccaga tatgctccat
     8221 gctggcatac ttcctaacca cacaaaatgg gttaggttct tctctaatct gaagttcatt
     8281 gtcgtggatg agcttcatgc ttatcgaggc gtttttggaa gccatgtcgc ttctgtcata
     8341 agacgattac ttcgaattgc caaattctat ggatcttcgc cgtctttcat cttttcatct
     8401 gccacgatcg gaaatccaca ggaattagct gagcattata tagagcgaaa ggttgagata
     8461 atagaagaaa acggagcagg aagtggagaa aaaattctag tttgcatcaa tccttctcta
     8521 atagacgaag tacagggctt aaggcgttcg aatgctctcg aagctgagtc tgtaatgcta
     8581 tggctattac aaaagggtat acggacaatc cttttttcta gatcacggct tcaagtagag
     8641 cttcttgccc attatctaaa tcagaaactc gaaaatccat ataatcgcaa ctatggtatt
     8701 gtggtaaaac catatcgttc gggtttgctt ccatcagagc gaagagaaat agaaagaggc
     8761 ttgagaaatg gaactgttca tggggttgtc tcgaccaatg ctctagagct tggtattgat
     8821 ataggtgggc tcgatgctgc tgtaataaca ggatatcctg gaagcattgc ttccttttgg
     8881 caacaagcgg gtagagcagg tcgaacttca ggcctctctc ttgctgttta tgtagcttct
     8941 tcttcacctc tcgatcagta ttttgtatct caccccgaat atttcctatc aagaggagca
     9001 gagcaagccc atatcgatgc atacaatccc tatatattta cagatcatct caagtgtgct
     9061 gcctttgagc ttcctttctt ggatgatgag gcattttctc ctgataattc gaatcgaaaa
     9121 gccgccgagc tcaccagtga agcgctcgaa tacttggagg aagaaggtat agtgcgtcat
     9181 gttgaaggta gatacttctg gtcaggagaa ggttatcctg gagaaaaaat atcgcttcgt
     9241 tccgccacta cagaaaatgt cgttatagtc gacataagca atgggcatca tgatgttatt
     9301 ggagaaatgg atcggccaag tgcaaaagag ctgctctttg aaaaagcgat ttatattcac
     9361 ctgggcacac agtaccaggt aaaaacgctt gatttcgaga agcgtatatg tcttgtagag
     9421 tcaagcgacg ctgattattg gactgattca attgtaaagc gcgatatcga actgcttact
     9481 gaggattcca ctgaatcgca tagcgagttt cgtcttatac ttggagacat tcttgtgcga
     9541 agccaggttg aaaagtataa gaaattgcgt ttcaacacac atgaaaatat cggatatgga
     9601 gagatatggc aggagccaga ggaaatgcag actcgatctc ttatgatggt tgtcgaccct
     9661 cagagtcaga gtggtaaatt cttgagccaa tttgggccag agctagctaa ttccatagtg
     9721 tatggtattg ctaatctgat acgccaactt gctccagcta aaatcctctg cgatatccac
     9781 gatatcgggg ttgcttctcg tgcaatagat cctttttata aaagccctgt catatatttt
     9841 tatgacatgt atcctggtgg tacaggaata gcagaggcgc tttgctcgaa tatgagtggg
     9901 attattagat ctacaattga aaggattgcc agttgtccat gccttggcgg ttgcccttcg
     9961 tgtattggtg tagaaaatgc aaatcctgat actaaggcgg catccctaaa gctcttaaac
    10021 ctgctttcat gttcttgaat tatttcaatc atctctgtat gcaacgacat agatattagg
    10081 caaataagga gttcagtaag tgaatcttaa agaacgcctt gctttgatta agagcgaaaa
    10141 aaccaaaaaa aacacttcag cttcaagaga aagaaatatg ccagaaaact ggcataatat
    10201 attgccttat gtatggacta gagagattca aggacctatc tttattgttc cttctttttt
    10261 ctatcttagt atcccaagat cattgcattc ctctgagatt gaagaaagta ttgattctca
    10321 ggctccagca tttcaaatag ccgccgataa aatagtattc ttcgatcttg agactaccgg
    10381 actctcaggt ggtgcaggga ctattgcctt tctttcgacc accgctcgct tcgaaggaag
    10441 gaatctaatt ctcaaccaag tattcctcga agattatcca ggagagcgcg attttctttc
    10501 agtggtgatc tcacaacttt cggctggaga ttgtattgca agctataatg gagcagcatt
    10561 cgatattcca ctgctccagt cgcgatgcat actaaatgga attataatgc cagctagatt
    10621 acatatcgat gtacttcaag actgccgacg attctggggt aaaattctag actcatgttc
    10681 tctctcctct atggaaagat tcatccttaa aataacaaga gaatttgata ttcctggagc
    10741 tgcaattcca aaagtttggc tggattatgt aaaaagcgat tctctcaacg aaaatcaaaa
    10801 ggcgctgatg gaaatggtat ggcagcacaa tattatggat gtggtctcgc ttgccaagct
    10861 atttttccat accgaatcgg cgtatcgcga tccatataga gctgttatcg aagatgcagt
    10921 agatccatta ggcctagttc atcgtctatg taagcttgga aggctagagg atgcaaaatc
    10981 actactctat atgatctata agaaccatga agacaatgat ttatctacag aaataatgag
    11041 aagagtgcat caatacctcg caaagctagc aagaaaagac aaggactggg aattattctc
    11101 agagatagta ctctctatgg atgaagaatt cctatatggg tgtgtggcaa aagcgaagct
    11161 cttcgaacat agattcaagg atgatgatac agctctaatc tgggctcgaa aagctcatga
    11221 tttagcatgc aattttggtt actcaggcgc aatcgaaaga aaggacaaag atatggcggc
    11281 ccagctctca gtcattgcct ctattgatca tcgtattgct cggcttgagc gtaaaatcgc
    11341 aaaaagaaag ggtatctcct agctgcccac aagctcccat tacaccgcgg ccacggctca
    11401 tccgtcgaac agttacgata cctctttcca aaaggatctg tgaaaatttt tcgatttgac
    11461 ggacagttgg agttttgaaa ggcattccat ttataggatt ccatgggata agattaacct
    11521 gagatttgat cggagtgatc cagttcgcta gctcaattgc atccttttct ctcacattca
    11581 aacctccgat aagaacgact tcaaatgtaa tgcgatcatt ggtgcttttc tgatattcga
    11641 gaagggctgg ttttagaaca tcgagaggcc atttcctatt gacaggcata agctctgttc
    11701 taatataagg gtcagcggag gtgagagaga cggctaggcg cacaattggg ccattttttg
    11761 caaggtctat aatgccagga acaataccac tagtggatat ggttattttc cgcatcgaca
    11821 atccaatgcc tgctggattc gagagaatgt aaatgctttt tctgacattg tcgagattaa
    11881 gcagcggctc tcccatgccc ataaatacaa cattggaaat aaatccacag cgctcgcgaa
    11941 gataatgaaa ttgttcgact atttcgtctg gtccaaggtt tctgagaaat cctaacgtgc
    12001 cagtcttgca gaatgcacat cccatggcac atcccacttg gctagaaaga caagctgtct
    12061 ttcttccttc gatatcttca agaagcacac attctacagc agctccgtcg cgaagcctta
    12121 tctgtagctt aagggagccg tctttatcag aaaactcgtt ttcgactctc gatgagcgga
    12181 cttggccatt gaaatgctcg gctagtcttt ctctaagagc taccgggaga ttggtcatct
    12241 cttcgaatga ccttactcct ctagataacc ataaaaatgc ttgttttgca cgatatagag
    12301 gttcttcaga taagatagcg accaattctt cctgagatag gccgctcgga tagactatat
    12361 ttagttcctc agcggaattg taattcatgt cttggctgtc agtttaaaca aggcatcctg
    12421 aataactgga ttttttatac ccatctgctg aaattcagta agtatttcga ctgccatttg
    12481 gcgctgatta ttcattagat aagtctgagc gagctctaca tagatacgat agttctttgg
    12541 atcgttttgt aagagcgagc gcaggctggc aatggcttca tcgtacttgc cctgctgtct
    12601 ggcaattacg gcgagaccta agactgcata gatgtcgaac tctatattga gagcattctg
    12661 gtaatattct gtggcttttt caaaatcgcc catattccga tatgcatctc ccgcgcgagt
    12721 cagaataacc ttattcttgg gatctttttc caagatctta ttccaataaa ccagggacat
    12781 attttgtttg cctactcctc gataacaatc ggctatgccg aaaagggcat agaagttatc
    12841 tggctctttt tcgagtgctt tctcaaagat tgggatacct ttatcgaaat gttttagctt
    12901 gcgataacag tttccaatgg cagtaaggac tctgatgtcg acattgtcgc cttccaactc
    12961 gaccatgcgc tgccagtaag agagggcttc cttatactcc ttaaaatcat agtgtaagtg
    13021 ccctaggcca ataagggcat agtgattatc tggattaagt tcgagaacac gatgataaat
    13081 agacttggat ttccgaaagt cacgaacctt acgatacgca tcagcaacgc gtgtcaatac
    13141 agtgatgttt gtgttgtcat ggagaaggta ttgttcccag atttcgatgg ctttttggaa
    13201 ttggttgaga gccttgtagc agtctgctag cccgaataat gcatagctgt tgcctggatg
    13261 aaaaactaag caacgtctat agtaatcggc agcttctttg aatcgctctt gcttgcgagc
    13321 ggcatctccc aagccaacaa gggcatagtt attctcttta tctttttcaa gaatttgaat
    13381 aaagcattcg actgcttcat cgaagcggtt ctcttttagg agctgatagc ccttttttga
    13441 gatttctgcg acctctgtgt tctctaattg gatttcatta ggaatctcga aaaaatcatc
    13501 attgtagggc aaagacggca tatcactcat tgctttttac ctcttggcgg aaagtatttt
    13561 taaatatggc tccttatggc cgaattattt attacctgac tctttccgtc tttctttaac
    13621 tctttttaca gtttttatta taccttttaa ttcatatcaa tgcaacattt tcgaagattt
    13681 aataataaat aagatatcat attcttctat aaaaatgcac aatagaacga ccaaagatcc
    13741 ttatatcata acattttagt cgagatattt gttctggcag cttgtcttca tgaggaaagt
    13801 ggatgaggac taggcttcca ttatgaatag aagcggatcg atcgagtcgt tcgagtagtt
    13861 gaagcttgaa tttatagtcg aaggggggat ctagaaaaat aatgtcaaat gcttgcttat
    13921 tgcgcaaaat gaaggtctcc gctggcatgg ccttacagaa tatcgcaggc tgagctatag
    13981 atgcattttg cagcaaaact ggaaattttt ttctatcttt ttcaatgcag acaacaggat
    14041 tagctccgcg cgatgccgct tctagcccaa ggattcctga acctgcaaag agatcgagaa
    14101 atgaatagcc ttgcaaatcg ccaaggacat cgaatacaga ttggcgcatc ctatccattg
    14161 aaggccttat tctcaagtct ccttcaggca ttttgacgat tcttcctgaa tattcgccgc
    14221 ctgttattcg cattgaagtt accaatcttt tactttgaat tctcgtctta tttcacgatc
    14281 cagatcccga gcttttattt cagctcgctt atcgtatgct tttttgccgc ggcagagacc
    14341 cagttcgagc tttataagac catgcttgag ataaatgctc aatggtatta aggtaaatcc
    14401 cttttctctg actcgccggt cgagtcgctt gatttcttga cgatgtagaa gaagtttttt
    14461 tggcctgtcc ggatcgtgac gaaaaatcga agaatgagga taatccgcaa tatgcagatt
    14521 attgagccat acttcctgtc cctttatctc cgcaaaggcg tcgctaaaac taactcttcc
    14581 ttctttgatt gactttacct cggaaccaaa taaaacaata ccgcattcaa gtctttcttc
    14641 aatatcgtaa tcaaaacgcg ctcggcgatt aatggctatg gtctttatgg agtcttgtgt
    14701 tttcattttc ttatcagagc aggtctaaat cctaaaaatt cggaagaatg atcggcaggc
    14761 atagggcctc gtgattcaat agagatttga tcagcattat tagcccacga gccccctttt
    14821 accgtctttg aatcgcctga attggcaagg atatcaaaag caggagagtc ttctacgatc
    14881 catttataag gtctaaaact atcagaagtc cattcccaga catttccaaa gagatcagag
    14941 aaacctgatg catcatggcc cgaatatcca acagaaatcg gcccagagga agaacgattg
    15001 ctgaatactc caagatccgc tatttttcga gaggcttggg ttgcagccat ttcccacatg
    15061 gcttctgtgg gaagttcgac tttatagcct aatggggcat tcttactgag ccatgcacaa
    15121 taagcattcg cagcatacca agagacaccc gttatcggct tatcgcttgg gcttgagata
    15181 tcaaagtctg caaggtagga actgtcaaca aatccctttc caatcaaagt tgcgcggttt
    15241 tctggcatcc attcaggatt ctcctttaaa aaacctgcaa attgtcgctg tgtaacttcg
    15301 gtgcttgaaa gaccaaactc gggaacataa gcaatatagg ggactctagt gccaccaggc
    15361 gtgattgtaa ggcctttcag atttcctcca ttaaacataa tgaaagagtg aggtccaacc
    15421 atatgtacac cgatagaatt atacagagtt gtctcaaatg tcgaagtctc tttttctaaa
    15481 gttgatagag ctcctcttat tgtatcatct gttctttcag gagctatttc cgaaaatgct
    15541 gctacatagt tctttgaact tgcaagcaat gcggtaacat tgcgagccat agatacaaat
    15601 cccaatggac ttgcgctagg cgcaccaagt gaaaagctcg caaatatcac atcacgaata
    15661 gacgagctat tctgagcagc gctcaagcct gctgcaagta gaggcttggc tatcgacact
    15721 ctatccgctg ggggcaaggc aacaagatct ttcgaagctt cagatagtac catcggtagc
    15781 tggtaaatag cgcttggtct accagaaagg gaccaagatg agaattctct aaaagatggg
    15841 ataagtatgg attccagcga ttctgcttct aattcatatt gtattctgac tctgggtggc
    15901 aatataagag tagcaaatat acggtttggt accttaatgt ctattgtttt aggctcgaag
    15961 ccacgcttgc gtatttggat ttgataagag ccaggtctaa gctttatccc atcagaagta
    16021 tttcctatat atttattttc tatgtaaaga gctgctatat ttggattacc ttcgaagctt
    16081 agcttggcgc ctgggttaga aaggccagga ttgatcaaaa ggaaaaaaag aatgataagt
    16141 attattagtg catagataat cggtatgtat atacgtggcc taatgccaaa gattggcttt
    16201 aaagttattt tgaccttttc aatatccaga tcggtgattt tttcactctc tttcattgta
    16261 aaagcagcct agccgatggg caccgtcttg tcaacgatat atgctcatga tagcttggaa
    16321 tcggaatgaa gaatatggat atttgcgata ggcttgttgt gttttctgaa gacttgctca
    16381 caagaaggaa acaaagatcc ctccgtgcaa agatgaaaca gcaaaagcgc catccgattt
    16441 tggactggat atatgctatt ctctgggctg cttgcgttgt acttgttata aatcagtatt
    16501 tttttcaaaa ctatcgaata ccatcgggat caatggaaaa gacacttcta gtgggagata
    16561 tgatttttgt agacaaactc tcctatggac ccgaactttt gcctggtgtt ttaaagaccc
    16621 caggaatttc caaaccaaaa cgagggaatg taattgtctt tgaaaatcct acctatctct
    16681 cgcgcgggcc aatctatacg atttttcaac aattgcttta catggtcaca tttacccttg
    16741 tcgacataga cagagaagca accggagagc cacgagtgca ttatctaata aagcgagcta
    16801 taggggttgg aggagatact cttcgtgtaa gaaatggtga agtatcaata aagcctatcg
    16861 gctcctcgga attcttagat gagcggatgt tgatggaagg tcttggattg cctgttaaga
    16921 tgcaaagatt agtaaattca agcgagtatt cagagataga caatgtcggc atagcttcgg
    16981 cctatgctga gcttgatctt cctcttcctt ctcgagtagg tatgccatct gtgcaaaatg
    17041 caaataagga cgcttttcaa tacgacatga ttcgtgtgac tactttacga gatgcagatc
    17101 cttccaattc ccgaaacgct cagcttgcgc agcgctataa gaatggatgg tatattgcgg
    17161 attcgcggat tttccctatg ggcgacaacc gagacaattc gcgcgatgca agatattttg
    17221 gaccaatcgc cgaaaaaaaa gtgctaggac atgccttgtt tatttacttc ccatttagcc
    17281 gaattggcag catccattga gagtgacgct gtatgttcgc caagcgcccc atacgatatg
    17341 cagagaaaat agaacggaaa aaggctcgaa acagaatact tatatacata actgttttta
    17401 tcattttgtg cattatcata aggaccttta tatttcagac ttggaagatt cgtggggacc
    17461 ttatgaaacc aacgctcaga gatggcgata tagttatagt tgtaccatgt ctgctttttg
    17521 cggaagaaaa atccatcggt atagccggct tgccaaaaaa aggtgatgtc gttcttgtgg
    17581 gagatgggtc ggaattcata ctaccctcta tagcaatgat taaagattca gtattgagat
    17641 ttattactct gcagaaatat tcgcttttgg caaccgaatt tggcaaaacc ttcagtcttc
    17701 ctgcggtaat gagaattcag ggtatccagc aggaaaagaa taacctcaag acatggacag
    17761 tctttaagct tgctacggac tctcaaggtg ataatttcag tatgacaggt agtcaaattg
    17821 ttttgccctc tcgaatcaaa ggcagagtag tcttgaggat atggccatta gctcgaatag
    17881 gaaaggtaca atgaagccaa tggctttaca tatccacgaa catgagggaa taacattttc
    17941 gaatatagat ccattcgaaa tacattcgct ttatgttcat attccattct gtacggatcg
    18001 gtgttcttat tgcgattttt tctccgtgcc gttcgcaaat aattcgcaca tttgttcatt
    18061 ggactatcaa atttcctatg tgcaagcaat acttttaaat tttcaaaatt ggtcgaagct
    18121 ttttcgggcg ggatcttttt cgacaatcta ccttggcgga ggaacaccat cagtactcga
    18181 taagcaagtg cttaaaaaac taatcgacag gctagaacca catgcagaca gatattgcga
    18241 gtggacaatc gaagctaatc ccgaaagtct tgacaaggcc ttactcgata tgctcgcttg
    18301 cacgaaagtc aatcgcatct ctctaggaat tcagactctt tcagaagaag aatggcctat
    18361 tcttcgaaga gtaggatcta tcgatgatgc gatacaagcc atagaacttc tatgcgagta
    18421 tccatttgaa atatctgtgg atctgctact aggtattcct catattcctc ataaaggtga
    18481 taaaaggcac aaaggcaaaa gctatatatt gcaatcactc gaataccttg taagtcgagt
    18541 ttcacacatt tcaatatatg acctaactct ggagaaagga actttgattg aaagccaagt
    18601 ttcttctggg gaatttgcca tgcttgatga agatgaattg gcagaagagc ggcgtaggat
    18661 tgatatttat ttagaaagcc aaggttttca tcgatatgaa gtttcgaact atgccaaatt
    18721 aggtcatgaa tgcaggcaca atgctgcata ttggaagatg gagccatatc ttggggtagg
    18781 atgtggagga gtgagcacaa tccaatatcc tgcttgcgaa aacagccagg agttaggcac
    18841 tattctcaga atcacagaga caaaagacat aaactcatat attcaatcgc caacggagat
    18901 tcctaaagac attgagataa tcgatagaaa gacagccctg ttcgaatttt tgatgatggg
    18961 tttcaggaca tcgcgtggga tcgataaaaa aagaataacc tcgctttttg gaataaatct
    19021 cgaggaatgc atacctttta ctctcaaacg atggaatagt gaaataatcc ggagtggaga
    19081 tcttattgct cttaagccat acaattttga tatactaaat cgatttctaa ttgaatgcct
    19141 tgaagaactc ggttgatgag gttttatatg gcaatttttc agtttgtagt ggtttcgtct
    19201 attctgcacg aagaaccaca aaaaatgaga ctgcttagtg cttttgagga taatcttcaa
    19261 agagctggag gcgaattgct gagtcttgga tctactttga tcaacccaag aaattctgca
    19321 ctaccgcttt tcattctcgt tctcacaggc ggaactgagt ctgaagccat gaggttgatt
    19381 tccaaagaaa gaatgctaga gctcaaaatg cccattgttc tattggctca tccatcgcaa
    19441 aactctctgt ctgcttctct tgagattctt gcaaagatca gacaagatgg cgggaaggga
    19501 ttgattcttc aagccgatac ctcgggtgca ttcgatgctg aagagctaga ggatataatt
    19561 gccgtttgtc agagcaaaat cgcaatgaga aactgcagaa ttggtgtagt gggtaagccc
    19621 tcggactggc tcattgcctc aagtcaggat tcgcttgtag ctctaaactc atggggtgtt
    19681 aagcttatca acattccttt ggaagagctc ctgcagaata tcggagagat acgttccaat
    19741 tccttggaaa ctcccattat tgaggattta caagaaaaag cagaattctt tgctgaatca
    19801 aacgatacag acttgctgaa gtcgatagag atattgcagg ctctaagaat ccttgtggat
    19861 cgatatagac tttcctcact tactttacgc tgtttcgatc ttgttctaaa agataagagc
    19921 acaggttgcc ttgctctatc tcaactttca gatgatggaa tcgatgcggg ctgcgaaggg
    19981 gacattcctt caattatcgg tctaagctgg attcgttctt tgacaggaca aattggctgg
    20041 atggccaacc cttctaatat aattgctaag aaagaggatg atttagccga aatgctgata
    20101 gcgcactgta cggcaccacg ctcaattcta tccaaatatg cgctacgcag ccattttgaa
    20161 tcagggcttg gagttgcaat cgcaggaatc tttcccaaag aagaagtaac tcttttcagg
    20221 atagggggca agtaccttga gaagctctgg tattctcctg ctcaagttat agaaagtccg
    20281 cgtagcgaag ggttgtgcag aacacaggtt ttgttaaatc ttcccaataa aaaagctcaa
    20341 gagctgctta caaatcctct tggtaatcat attgtaatgg taagaggaaa ttggaccaaa
    20401 aggatttcga tttttgagca actctaggct tttcgggatt atttaaacaa agaagtcata
    20461 gtttggtcgg atacgctcag caaagccttc ttggaaagat atattggcgc ttttttccgt
    20521 accaatggtg cttggtgggc catgtccagg gagaacaagc gtactctctg gaaggcaaaa
    20581 gagtttttgc ttaatgcgag ctataagcgc atttgtattg aagacactat tcgtttttcc
    20641 aatcatgcct gcatggagga catcgccggt aaaaagaaaa tttccttcca tgaaatagac
    20701 aattgaatcc tgagaatggc cgggtactgc tattgcggta atctcgaagc ctgcttcgct
    20761 aaaaacatcc ccatctctca actttctaca ctgaaatcca agtaatttcg cattagaagc
    20821 atagattgtg gggctataga tcttcatgat tgtagatagt ccctttacat gatgaatatg
    20881 gttatgtgtg atgagtattc cgcagagatc gagtttgttt tgctcgattc tttcgatcat
    20941 tgtcggagtg agttctgcag gatcaactat tatagccttc tttaatgatt cattgacgat
    21001 tatgtatata ttcgaaaatc catagaccga ataatgatgg tagatcttca tagataaaga
    21061 tgctccagat ctcggacagc ctctgcaata ttcgatcttg agaagctgac tttttgctct
    21121 ttagaaggaa tatagaaggc tctcttaaaa tcgcaattgt caatatttac agtattcatg
    21181 tcacacatta taaaacgaga gttatataga ttagagcctg caaaggtaca atccacaatt
    21241 acagagccat tgaagcttga ttggataagc tcggagttct caaagctaca gtccataaaa
    21301 gaacatttcc caaaggaaca aaaatcgaca tccgaaccag atagatcaca agaaaagaag
    21361 ctggaactat ctaaaaaaca taattggaaa gaagccccag taaacaatga atgttgaatt
    21421 cttgcatttc taaaagagca accaacgaaa cgatagcgag aaaaatcaag acccataaga
    21481 aacgcataat caaaaactct attaagaata gtattattgc tctgcaactc ctgcatggct
    21541 cttttcatgc gctcaggata gtcttgtagg tgatgataac agaggttttc tatcgagaaa
    21601 gcaggcaaac tgcaaccttc tattgcgcaa gaagaaacct tatacataat tacagcctat
    21661 atgatcttgc aaggattctc aagactcaaa aacaaaaacc taaaaacata tatcaaacat
    21721 tctaatcatt catattgatt taaagcgctt gtcagtattt tgcttacaat gtagaatttt
    21781 tgatatgtgt tataaatgcg gtgccctatt ggcctatgat tataggccag ggttcaatga
    21841 tacctgtgat agatgcggaa aagacctgca cgtgtgccta atgtgtcgtt tctatactcc
    21901 tggtgcccat tgggactgca gagagacaat agaggagctt gtagcagata aagaaagacg
    21961 aaatcattgc gattttttca ttatttcgga gaaatatctt gacaaaggga gaactgcaga
    22021 ttcaaagcaa agcgatgaag cgaaaagaag attcaattca ttgtttggtg aatagatatc
    22081 taaggagaaa tgagcgatgc gcatctgcgt atttgattcg ggcatagggg gattgccttt
    22141 tgttcaggct ttaaatcgag agtttccctt cttagaaata gattatgtag ctgatgatgc
    22201 aggcttcccc tatggaacaa aatctcctga gatgatccga gatatgctct ttgaacgctt
    22261 aaggagaatc cgagcacggc ttgatcccga gattcttata atctcatgta tgacagccgt
    22321 tcaaatagga ttaaaagagc ttcaagctgc tcatagagca ttaaatataa taggagcaat
    22381 acctcctatt gcagaggcag cgaaagaatc acagactcat cgcatagcgc tgctttctac
    22441 atctcgcact gccgaggatt cttttctaga ttatttaata gccagagatg ctccagatag
    22501 tgaagtactt agaatacctg cccaagatct tattgaatat gcagagcaac atcttccttt
    22561 tgcgtctttg ctctctgcgc agacaatggt agctccttac atcaattatg cattagagga
    22621 aggggcagat cgaattgtct tggctagcag ccaattcatt ctccttgaag atgctattgc
    22681 cagtcaattg aacgaacgac aggctttcaa tgtccaatgc cttgatccaa gggcaaaagt
    22741 gatatccgct ttacaaaagc ttattcatta taaagagcat gccgaagaaa ccaaatttca
    22801 aaagccaaga ttctttctag ccggggatcg tggtgcaact ccttactaca ttgcatggtc
    22861 agataaattc aattgttcta cgccacagct tctatgaatc gcaatagcaa cgaatccatg
    22921 gtactaggtg tagtaatctc attcagcaat cgatatgcaa aagtgaggtg cgatgatgga
    22981 aatattcgaa catgtacgat caaaggcaag agaatcgact catcatctag aggttataaa
    23041 aatcttgctg cgggcgacca tgtatcagtg ataccgttat cgaaacagaa tggtgttata
    23101 gaatctattt tcgataggaa aagtgaattc ggtcgctata acgagaaagg agaggcacga
    23161 caggtatttg cagctaacct tgaccttgta gtatgcatag cgagtacaag agcgcctcct
    23221 tttaggccta gatttatcga tagagtgggt gtaatggctg aatattgtgg cctgcctttt
    23281 cttatagtat gtaataagat cgatctaggt ctggatattt caatcgaatc tcgtctcgcc
    23341 gcttttcagg agcttggcta tggagtaatt aaaagtagtg ttcgggattc tataggaatc
    23401 gatgaacttc ggaaggtcct tattggaaaa acctcaatct ttatagggca atccggtact
    23461 ggcaagtcga gcatcataaa ttgtctgatt ccaggagcag atcagcgaat agccgagatt
    23521 tcggaaaaat atgatagagg aaaacacacg acaactctct ctacaatgct ctcttcgcca
    23581 aacgaagatt ttaacataat cgatactcct ggtattcgta ggcttgcgat acgaaatata
    23641 gaaccaaata atcttgcata ttattttcct gatatggttc cgtttttggg tctatgtgag
    23701 tttggtgctt cctgtacaca tagatatgaa ttgcactgct ttgtaaagca agctgtacaa
    23761 gaaggactca taaataatga ccgctacgag agctatctta gaatgcgtgc tgaattagaa
    23821 gaaacaaaaa atgccaagac aaacgaagcc aggaggttac agaggtctgc tacagatcaa
    23881 ttcgaggaag aagaatggtg actaatgagc atagccttgc acttctagat ttttatcgac
    23941 tgcgaaatcg tctggaagaa tactgttttt ctcacgaagg aagggcactt atgaatagca
    24001 ccttgccttg ctctcacgag gattcagtaa aaagactaaa gaaagactta tcgctgctcg
    24061 ttgaaggact tagcgataaa gaaatgccat catttccttt tccaaatatc gagattccca
    24121 taacaaaagc cctaaaagag ggcacaatgc tcgaagtcga tgagcttttc gctatagggc
    24181 tctgggcaaa atcctatcag caattttttg cctttcttcg ttcgcttatt aatcctcttg
    24241 ttgaagtaaa tcatgagacc tgggaagaag attttagtaa tgacgaggct tgggcaaagc
    24301 tgggaattga gaatatagtg cattcggctc cttctttgat taatgctgta caaatagtat
    24361 ttgctgttat ttctgaggat ggcgaaataa aagatttgcc tcgcctgaaa tctctaaagc
    24421 tggcaattca aaaaactcaa gctgaaaatt tgcacattat aaatcgatat ctttctgatc
    24481 ctacacttca agatgccctt caatcgaaca tagctacaga acgagataat agaaccgtaa
    24541 tagcaataaa ggcgaatttc aaaggaagaa taaagggaat cgtacacgaa ttttcttcga
    24601 caggtcaaac aatcttcata gagccacttg agctggttga aaggaataat aagctgctgg
    24661 agctcgattc taaactaaac gaagagataa gggctattct aagagagaca acagaggcac
    24721 ttaggcaata tttgcctatc ctaatacgtg gcagaggact tttagcctct gctgatgtta
    24781 ggttggcacg agctatccaa cttaagagag aggatctcgc aattgcaggc aatcttgaac
    24841 aaggtataca tctctatcaa gccaaacatc ccttactcgg caaaaaggcc gtacctatag
    24901 atgtagatat tccagatggt gcaagaattt taataatcac tggtcccaac acaggaggaa
    24961 agacagtatc tctaaaaacg attggccttc ttgcgctttt gaatcaatat ggcgctggca
    25021 taccagcact atcaacttca gcatttgcag tttttgatac aattctcgct gatataggag
    25081 atgaacaatc catagatcag tcactgtcta cattttctgg tcatatgaat attatttctg
    25141 gaattgtatc ttcagctact gatcgcagcc ttgtattgct agacgagcta ggagcaggaa
    25201 ccgatccaga ggaaggttgt gcaatagcca tggccttact cgattatttt caccatttgc
    25261 aatgttttgc catagtcagc actcatcatg gagttctgaa gaattatgga tatactaaac
    25321 ctggctgttt gaatgcctct atggaatttg acgcagagag tctcagcccg aattacagga
    25381 taataatggg ggttccagga gaaagcagag ctctcgagat tgcaagccag atcggtttac
    25441 ctaaagaaat tgtcgatgta gctaaagctt atctcgaaga tgaacgatca gattattcag
    25501 taattattca ttctctagga gaaaaacaga aggagctcga aaagctcgaa ttagaaaaga
    25561 aaaggcaact aaagaaagta atcgattcgc gccgtgaagt agatcttgag aatctcagga
    25621 ttcgtcaaag agagcttgaa cttagaaaac agggagttgc tgaacttaaa aattttctga
    25681 ctgaatctag aaagattttc gaaaacattg tgcgcgagtt gagagaaaac ggtataaaag
    25741 atgaggatac gtcttcagga cgggaattct tgcgaaaaat gtcagacgag attcaccagc
    25801 aaagcgaaga gcttgaaagt ttttcagaag agactcaaag aattgaacgc ttgatgaaac
    25861 ctgatatgca aagcagtata ccgatagata tcgataaaat aaaatctgga gatactgtca
    25921 tatttcagga tagagaagct attttcctta gaatgctcga tgaaaagaag gcccttgtac
    25981 agctgggctc tattaggatc ccgattgaat gcgaagctct gagtcttcca cgatcaagga
    26041 ataaaactga gatagtttca aagcttggct accagattga actagcttct gattcgatgg
    26101 caaagagaat gcctgtagct gaactcgatg tgcgtggcat gaggctttca gaggctctcg
    26161 atgctttgga gcatcaaatc gatgctgcta gtctcgctgg tattagcaat tttgccataa
    26221 tccatggaac tggagaaggg attctctcta gaggtattca cgagtggtta agcatgcaga
    26281 gctccgttgc ggattactat tttgccagac cagaagatgg aggtttcggg aaaacctgga
    26341 ttcatctcaa agaataagat aaaatcctca aattttacta atttctcgct tttttgtttt
    26401 ttaaattgac attgcttgaa ttttgctgtc ctattctgtc aattgacacc aatttatgca
    26461 acatgatatc gttatttggt tttcttaaag ccaaaggagt tttcatgtct ggacatagta
    26521 aatgggcaag cattaagcat aaaaaggggg cacttgatgc caaaaggggc cagcttttta
    26581 caaagttaat caaggagatt tcaattgcag ctcgcatggg cggaggggat atcgaaggta
    26641 atccacgcct tcgtacagca gtgttgaaag ctagggctgc aaacatgcca aaagacaata
    26701 tcgatcgagc tataaaaaag ggtactggcg agcttgaggg ctctatctat gaagagttat
    26761 tctacgaagc ttatacgcca ggtggtggtg caatgctcat cgaagttctc accgataata
    26821 agaatcgagc agccgcggaa atacgaaaca taatcacaag atcaggggcc aatttgggga
    26881 ctaccggatc ggtcgcatac ctttttaagc gcaaaggcgt cctaacctat gatagcgaaa
    26941 aatacaccga ggatcagatc atggaagccg ccctagatgc tggagccgat gatgtggtca
    27001 gcgaagatgg tactatttcg gtatacactg atcccgcttc ttttgaggat gtcttaaata
    27061 tgatgaacga taaaggcttc gaaacactag gagccgaaat ctcgatgctg ccagactctt
    27121 atataactgt cgatctagaa acagctcaaa aaatacaaag actcatcgat aaactagaag
    27181 aaaacgaaga tgtacaaagt gtttatcaca acatcgaaat gcctgagctc gaggaatgat
    27241 cggttccatg aattctcaaa gttctctcct ggcaatcgga atcgatcctg gaatagcatc
    27301 gatagggtat ggccttgtga gaacttttga aggaaggctt tcgctgattg cttatggttg
    27361 cattgagaca gaatcggacg aaccaattgg gaagagactg acctatatat ttgaagagat
    27421 aagaaaactt cttgggcaat atgctcccga tattggcggc atcgaggagc tttatttttt
    27481 tagaaatgta tcctcagctt tccctgtagc agaggctcgt ggcgttatta gacttgcttt
    27541 tgaaaagacg ggtgtagtcc taagggagta tagtccaaat gctataaaaa aagcggtgac
    27601 aggctcttcg agggcaagca aaagccaggt tcaggagatg gtcaaggtgc ttctaggtat
    27661 gtctcagata ccgacccccg atcatgctgc ggatgctctc gctgccgcta tttgtacatt
    27721 gcattctatt ggtcctgcgg gctatactct ctaacatgtt caacagaata aagggtgtat
    27781 tcagcggtta tgcagatgaa gcccttcaca tatccacggg agccatagaa tgggaaatat
    27841 tcgtcccaat ccgtgatcca tccttttaca cccacacagg agaagaaata gagcttttca
    27901 catggctaca tcactatgaa gacgcgatgc gtctatatgg ttttttttct gcagtggaac
    27961 gccagctttt tctcgatctc caaaaagtag aagggattgg tcctcgccaa gcctttaaga
    28021 ttttacaagg cattgcgccg cgctccttag ccacgatgct cgatagaggc gacctagcct
    28081 cattgcaaaa aattccgggc ataggtccaa agacagcaca aaagatgatg ctagctctaa
    28141 aaggaaaact gattactcct gaagaggctt ctgctgcaat atctaactct caaggacgat
    28201 tcagagatgt aatccgtgcc ttagttgaga tgggttttga acgaaaagaa gtagagcgcg
    28261 tgacagctca gctcgcttca tctttagaag atggtcctga agcggaaaaa gaattgttca
    28321 aaagggcact tcttgagctg tccgcaggag ccggagcatg aatcctccta caatagatca
    28381 gacctcttcg cccagtacaa tgaatcccct ctataatcca gcagaagacg aaaaggaagc
    28441 ctctctgcga ccgcgctatc tttccgagtt tcagggtcaa cagagaataa aagataattt
    28501 gctcatcttt attaaagctg cgcgcgatag gggtgaagcg ctcgaccatc tatttctgat
    28561 gggacctcct ggtcttggca aaacaactct tgctcttgtt gtggcatccg aaatgggcgt
    28621 tgattttaag cctacaagtg cacctgctat tgaaaagccc aaagatctgg ctggcatttt
    28681 gactaatctt aggcccgctg gtgttctctt catagatgaa attcataggc ttaaaccatc
    28741 tattgaagaa atgctctaca tagccatgga agatttcgag ctcgattgga ttataggaca
    28801 aggtcctgca gctcgtacgg taaggatccc tattcctccc tttacactcg taggagcgac
    28861 aaccaaggca ggtatggtct ctgctccact ttcaagccgt tttggaatat ctttgcgctt
    28921 tgactattat tctcctgaag aattggcaag tgttattcgc cgttcctcga atattctcga
    28981 tatcaaaatt aaagaagatg caattgaact cctctctgga acgagcagag gaacgccacg
    29041 catagctaat aggcttctac ggcggatgcg cgattttgct caggtgcgag gagaaggtat
    29101 tataacgaga gatatcgtag aggatggact cgaaaggctg gaggtggatt atcttggcct
    29161 cgaacgcctt gatcgcgaaa ttcttaagac aataatctca aagtttggag gcggacctgt
    29221 tggggctgaa actctagccg tatctgttag tgaaagcgtt gagagccttg aagattatta
    29281 cgaaccatat cttattcaga taggcatgct agcccgtacg cctcgtggaa gagtggcaac
    29341 tcctatagca tatcggcacc tcggaatgga aaaactagat gaaaacggac agtttcaatt
    29401 ttgatctgcc gaaacattta attgcacaat accccacaga ggaacgaggc agatcgcgcc
    29461 taatggtttt ggatcgcaga actggaaaca tcgtagattc catggtggaa gatgtggcct
    29521 cctttattga acccaatgct ctgatggtgt tcaatgactc gaaagtgcga aaagctagat
    29581 tatatggaag atgtgaacag acaggaactt ccgtggaatt tatctttctt tctccatgga
    29641 aacaagatga cttaataagc cttcgaggct cagaattcaa tcatgaaggt caagagaatc
    29701 ttgtaacagc ttgtgaacaa gtatcatctg tctcgcgcct atggcgagct gtatgcaata
    29761 aatcgaaacg ccagaatata ggccgttcat atatatttcc agggcagttg agaggttcag
    29821 ttgtcgaaga tagaggagat gaaaagatca ttgaattttc ttctaatata ggcgaagaat
    29881 attttgatct ttacggacat gttcctctgc ctccttacat aaagcgagaa gatacaggca
    29941 atgatgaggt acggtatcag actgtgtttg caaagcatac tggttctgcc gcaagcccta
    30001 cagctggatt gcattttacg ccccaactac tcgatgagct ttctcagaaa ggtatagaaa
    30061 tcgaatacct taccctccat attggtctgg gtacttttca acctgtaagg gttgagaata
    30121 tcgaagatca tcatatgcat gaagaatggt tcgagattcc gtttcaagtt gccgaaagaa
    30181 taaaccttgc tttaagagaa ggcagaaaag tgctggcagt aggaactact tctatgcgag
    30241 ctttggaatc agcttggaat cggggacaag ttaggcatgg caatttttcc acaagaattt
    30301 ttatctatcc aggttatgaa ttccatgtag ttaatagtct tttcaccaat tttcatactc
    30361 caaaatctac tttgataatg ttggtgtctg cctttgcggg gagagaaaag atcatgcaat
    30421 catatcaatt agccatagat aaaggctatc gatttttcag ctatggcgac gctatgctta
    30481 ttctatagcg tttttagaga tgttggcatt tacgccaaat ctatagttta tggcggtgga
    30541 acactcaatt cacacaattc agcattcaac gccgagaaag cttaaaacga tagattctat
    30601 ttgctgatgc acagattcga tatctttgct accatcaatg cgctctatgc gccaaccctt
    30661 atgagaaaga cgatcagcca ttctctcata catgttctgc acttttcttt ggaattcgag
    30721 ggtctcatag atctctttct ttaatcgaga acgaatgcga gttatgcaga tttctggatc
    30781 tacttcaaat aggagtgtca agcctggagc tggaaaagta ctattcaata accaaggcag
    30841 tctaggtcca catgttgtgc cttgataggc tagactagat agaacatatc tatctgaaac
    30901 aaccattgaa ccagccttga gactttcgat aatgccattt ttcccgtata gatgttcatt
    30961 tcgatcagca gcaaatagat gtgcaactgt agtaggagca actatatctt cgccagagag
    31021 aatggtacgg atcagttggc caatatgatt cattgtgggt tctgccgtaa ctatgcaaga
    31081 aaatcctccg gcaattaggc tctctgccag taagcgaact tgggttgttg ttccagcacc
    31141 atcgattcct tcgatgacaa tgaaacgatt tttgaactcg tctgtgttat atgaaggcat
    31201 attatgctag tatataggcg actttaggtt ttgtcattca ggatttgcgt tgaatgaaga
    31261 cgaaaaaatc tgtaatacta atcgtcttat tttctctaac tcttgcaatc tgtggcagta
    31321 ttgcgattat cgatctcgtt caacgtgaag tgcaggtact agcgattaga gctttagata
    31381 aactccagtc aacaatagtc gatactaccg gtcttattct gaaatatgat tatttggata
    31441 tcgagtctct aaatcgattt tccatgcatg gcattgagct tattcgctca gatcctctag
    31501 atcagaataa tagcgagttt ccatcatatt tggagttctc tctaccatca gaaagatcaa
    31561 attacaaaga acagccacca ctgaagataa ataaactctc agtccggata agtttttggg
    31621 ctttatttgc cggtcaaaat aatgaagctg tgagagacat cacagtagat gatgtccaga
    31681 tttcgcttta tttccccaac gacgaataca tcataaataa gatactagca aatttcgttt
    31741 caggaaaagc aaaaagcctt ccacattttg aactatcaat cgggcctata cgcgctgagg
    31801 tgcgagagat tgcgaaacag cccaactcat ccatagacaa ggatgtgatt aatggaggaa
    31861 gcctgaccat agaatctctc cagttttcga gcttaaccga ttctccagtt atcttggcac
    31921 cctctgtgat tttgaaggcc aatggccttt ttggtttgcc taaagagctc aatgcagaat
    31981 ttagtcttag gggaagcgga aatgctgatt tatccaatat cgatatgaac atagagcttc
    32041 aggcaagttc tacgaactgg cacattatac cgcaacaact cttactgcaa aagcgaggga
    32101 gtaggataga gtgtgagagg gaagggggag gtggtttagt taggggttgg tatgagagtg
    32161 gggagtgggg tttagagggg gtatttgaag ggtacagggc tggggaggac ataggggtag
    32221 gaggggtatg gggtaattta gtaaggggta gtgaggtaag tggtaaggta gaggtagggg
    32281 gtaagggtaa ggagctagtt gggtatgagg tagatttagg gattgaaata ggggagggta
    32341 tagagatatt tggtaaaggg gtaggtggaa taggagtaag gcttaagggg gtaggggata
    32401 taggcaggta tataggggag gttagtgtag ttaagggtgg gggtaaggtg aggtatgagg
    32461 gggaggtagg gtatgagggt ataaggttag taggcaggta tgaggtgagt ggagagggta
    32521 tagggataag aggggaggta agaggaggtg ggggggcata tgagataggg gtagagggag
    32581 ggggagtgaa gggtataggg gtaggggcag gtatttgtaa agttaaggta ggggaagaaa
    32641 gttatgaggt aaggtatgag ggggttatag ggggagggga agtgaagtta gggggtagta
    32701 taggtaagtt aggaggttat gagggggttg tggagttaga gggggtaggg ataggtaagg
    32761 tgatggaggc cataggggta agaggagtag aaggtaaggt tagggggcgg atatatgggt
    32821 tagagagggg agggaagata tcttggagta taagtgggtt agggtatgag ggggaggtga
    32881 tgggtatagg ggtagggata agaggagaag gggtagggga tgagaaggga tatgagataa
    32941 aggggttgga ggtaagtata ggtagttatg atatagggtt gaggggtaag gggatatatg
    33001 ggggggagtt attttctggg gaggttagtg gaggggggat gaggtatgag gtgagggtag
    33061 ggataagggg tggagaggta agagtagagg tagggaagga gtgtagtggg gtatttgagg
    33121 tatctggggg tcgtgtaggg ggggaggtag ggatggataa ttttggtata gggatagggg
    33181 atggggtaat gaggttaggg ggcaaggcta ggggttggta tgagggtggg cattggggga
    33241 taagtttaga tgggattagt ttgaggtatg agggagaggg ggtatatccc gagttaaggg
    33301 ttagcggatc ggcgggagag ggagggttac gatttgagca gctagaggta ataggggaag
    33361 ggtatggatt taaggggtct atagatctca aggaagaagg ggggaggtat atatttattg
    33421 ggagtgggag gggtgaaggg gccttagggg aaggttctga gtattcgata tggggagggg
    33481 ttaagggcaa gggagtagag ttgagggtag gggtacaagg tttagaggta agagggtatg
    33541 aggtagaggg atatgctgat ttgatagggg aagtggacat aggggagtta ttgaaggggt
    33601 cttgggaggg gttaggggac ataagtgggg aagtagcgat aggtttgagg ggttctgggt
    33661 taagtgtagt ggagcaacgt tttaaggtga gcaagcgagg gagtaggata gagtgtgaga
    33721 gggaaggggg aggtggttta gttaggggtt ggtatgagag tggggagtgg ggtttagagg
    33781 gggtatttga agggtacagg gctggggagg acataggggt aggaggggta tggggtaatt
    33841 tagtaagggg tagtgaggta agtggtaagg tagaggtagg gggtaagggt aaggagctag
    33901 ttgggtatga ggtagattta gggattgaaa taggggaggg tatagagata tttggtaaag
    33961 gggtaggtgg aataggagta aggcttaagg gggtagggga tataggcagg tatatagggg
    34021 aggttagtgt agttaagggt gggggtaagg tgaggtatga gggggaggta gggtatgagg
    34081 gtataaggtt agtaggcagg tatgaggtga gtggagaggg tatagggata agaggggagg
    34141 taagaggagg tgggggggca tatgagatag gggtagaggg agggggagtg aagggtatag
    34201 gggtaggggc aggtatttgt aaagttaagg taggggaaga aagttatgag gtaaggtatg
    34261 agggggttat agggggaggg gaagtgaagt tagggggtag tataggtaag ttaggaggtt
    34321 atgagggggt tgtggagtta gagggggtag ggataggtaa ggtgatggag gccatagggg
    34381 taagaggagt agaaggtaag gttagggggc ggatatatgg gttagagagg ggagggaaga
    34441 tatcttggag tataagtggg ttagggtatg agggggaggt gatgggtata ggggtaggga
    34501 taagaggaga aggggtaggg gatgagaagg gatatgagat aaaggggttg gaggtaagta
    34561 taggtagtta tgatataggg ttgaggggta aggggatata tgggggggag ttattttctg
    34621 gggaggttag tggagggggg atgaggtatg aggtgagggt agggataagg ggtggagagg
    34681 taagagtaga ggtagggaag gagtgtagtg gggtatttga ggtatctggg ggtcgtgtag
    34741 ggggggaggt agggatggat aattttggta tagggatagg ggatggggta atgaggttag
    34801 ggggcaaggc taggggttgg tatgagggtg ggcattgggg gataagttta gatgggatta
    34861 gtttgaggta tgagggagag ggggtatatc ccgagttaag ggttagcgga tcggcgggag
    34921 agcaagaagc caatctcaat atagattatt tacaatatgc tggacaatcg ctaaaaggtt
    34981 caatggctat ccaatattct tcgatagcta atctactaaa agatacaaaa ataaattatt
    35041 cattcctgag cattgaaact aataggccaa tcctagaagg aaggattgta gctctagaca
    35101 acaaccttga agtttcaatt atcgcatcat caattccatt ggaagaaata ttgccatcta
    35161 ctcttaatat ttctggagat atagagttaa agggaagtgc aaccatttta atatcaggta
    35221 aaaagttcaa tctttctgat atatcttatc tgggtctgca attcaacact agaaaagcag
    35281 aaatgaaagg aatatctttt tcagcttcgg ctgtaataaa gcttaagggt tctgctttat
    35341 ctttggaatc tggatttttc tcttatcaga actatagatt tgaaaatata aatgctgaat
    35401 acgatataga tacaaaacaa gtcacctatg atctgaattc taagatagta attggtaaac
    35461 aattgctaag cgctaggttg aaaggcatag gcgaaaatgt ggatgactct atatttaaac
    35521 cagatatggc tcagaatttt aatattatcg gggatataat tgaagcaaaa ttcggatcta
    35581 gagctataga tccactaacc tactcaattt cttatttgga acacaaccta cagattcatc
    35641 taagccaagc aaacggggac agcatcgatg cctcgataaa taatatgaca gattttgatc
    35701 ttgtcttaaa tgatttattt aaggtcaaag gaaaagctag tggtaaaatc caagcaaacg
    35761 aagtatcagc tgacattgtt ttggagaaaa ttgatttaac atcgctagga gctttgatct
    35821 caaaagatat tttacgagat atctcgggag acgcatcggc tgtattgcat ctctctggag
    35881 atatatcaga ccctagaatt gaaggcagaa tcttattcag caattttatt atcgatccga
    35941 atctatatgt tctagaaaaa atgggacctt tcaatgcaga aattactata agtgatgggg
    36001 tagtggaatt aagtccaaca attgtgaata ttggaactgg cgatatttcc cttgctgcga
    36061 ctgcgaacct tgctagatgg caactcagcg atatcaaagt ctttttttca actcttgaga
    36121 aagcttctat gcgcttaaaa ggaactattg gtgggttgaa tactgatggc atttggatta
    36181 aaggcgatat aaaagcagca atatcttcgg attttgtcga aattggcggg aatatacttc
    36241 tagataatgg gacacttgaa gttgatccta atggttttgt tttctcagaa aacacccaat
    36301 ctccagtttc gcctctttat ctcaatttaa ttgttacatt tggaaaaaat gtccaaatgt
    36361 atatacccaa caaggatata tcacttatta aaggtactat atctcctact tcgactttga
    36421 aagtgcaata cgatcaggca aaaggagatc ttgctgtaaa tggtagcatt gagcttcgtt
    36481 cgggatacat atattatctc ctgagagatt tctttataaa agagtgcaca atagaactct
    36541 cagaaaacca atccaaattc aatccagtta tgactgtcat tgctgagttg atggagccca
    36601 gcaaggatgg aatgatcaaa ataacgcttt cagccaacaa atctcctata gataacttca
    36661 atccagtatt gtcttcggtg cctccaaaaa gccaaatcga gcttcttgct cttcttggtg
    36721 gtggattagc actttcagag ttagcagaga atactcctct tacattgaaa gaagcagtaa
    36781 tcgcaagctc cgagtttctt acacataatt tggtttttag atcattcgag caacgagttc
    36841 aaaaggcttt aggccttgat gttctttacc tgcagtcatc ttttattcag cgttggcttc
    36901 tagatataac agatgaggcc cataaggatc agactaattt atcgaagtat ctaattggaa
    36961 cagggctttt tggaggaaaa tatataaccg attcagcttt tgcgcatttt tctcttagga
    37021 tagagcagaa cccgcttgag aaaactggat ttttacaatt aaatccagaa attgggctcg
    37081 aactgcaatc tccatttggt cttttgaaat ggagtatgtc gtttggtaaa gaaggcatgc
    37141 ccttaaataa ccaagacctt agtttgagtt ggcgtatcaa tttttgagaa aatacaggag
    37201 cagcaatgaa atattcgaat aggcttttca tctcaatcat aatgattttt tgtgcttttc
    37261 aaataatttg cgctcagact tcgacagaag agcttcctca ccaagataat tctcaggatt
    37321 ggttttggaa taaaaaaata gctggattca aatgggatgg attacactat gcaaaccgca
    37381 atgatctcga ttccatgctg cgtggctaca tagggacatt ttttactgag aatatttgga
    37441 tagaaattca agcaaagctc tatgcgctag attggtttga gagcattgaa tctatcgcat
    37501 taccagctgg agatgaagaa gatagaataa taattaaatt catagtgatc gaaaagccat
    37561 ctatcagctc tataaaagtc attggaaaca gttctatcag aacgtcggaa ttactcgatg
    37621 tcgcttcttc gaaaaccggt gcaattttta atgaagataa agcaaaggct gatgcacttg
    37681 cgatccaaaa attctaccta cagaagggtt tccccgatgc aaaagtggaa tatgaaaaaa
    37741 atcctgctgc aggaaatcct aacctaatag tgttgacatt caaggtaacc gaaggaacag
    37801 ctgttgttgt tagaaagatc gcattctcag gtaattctgc attttcttct cagactctca
    37861 aaggccaggt aacacttaaa gaagctgggc tttttcagaa aggagcatac gaagaatcaa
    37921 agctggctga aagccgaaca gctttagaag attattatgc atctaaggga tatgtagatg
    37981 ctaaagtcat agatgtaatc aagaattatt ccaaagatga aaaaagtcag aaaaactata
    38041 tagacataac ttttgttata gccgaaggca agcaatggag attaggtgat attgtctttg
    38101 aaggcaatag tatattttct acatctcgcc ttgcttcttt gatatctcta aaacagaatg
    38161 aacccataaa tctgaagcgc cttcttactg acaaacaaaa aatagacgat ctatattatg
    38221 aatccggcta tattttcaat aatataacaa tgactgaagt tcgagatgaa cagactggaa
    38281 aaatttcatt caagatctcg atcatcgaaa gggatagggc tcatatagag agtattacct
    38341 tcaagggcaa tacaaagaca aaggattatg tcattgcaag agaaataccg cttgaagctg
    38401 gtgaagtatt ttcaaagaca aagatcatcg atggactacg aaatctatat agccttcaat
    38461 acttctcatc tatcgagcct gagattcatc aaggaagtgt aatagacttg atggatctgg
    38521 ttataaatgt agaagaaatg agcacatctc aaatacaatt tggtcttacg ctatctgggc
    38581 ttggacaaca aactgctttt cctatctcag gatttgtaaa atttagcgac ttgaatcttg
    38641 ggggcacggg aaagaacctt caggtcaata tgacactttc tccagatgaa caaagcctag
    38701 agacttcatt tggtcagagc tggcttttta ataagcgaat atctcagagt cttagcttca
    38761 atatagcgca ttcaatcctt cagactgcac aagattctat agctcctatt ttctctgagg
    38821 aagacattcc agatccgttt attgcgcttg gaagtggatc aaatgaatgg gatggccttc
    38881 tttcatcggt ttcagatact tacttgatgg attacgatga ttatgctttc agccttgggt
    38941 attcgatggg ctatgttcat aaaatatcgc aaggggatgt gggaatttct gggggtgttt
    39001 cttcaggctt agatatgtct tcctatgaca gcgccaaata tcgcccttac gaaaaagaaa
    39061 tgcgcgacta taatggtcaa tgggtattaa aaaattctat ttttggtcgg gtatatctga
    39121 ataagttgga ttattggtat aaccctggga aaggatactt tttaagtgaa cgtctcgctt
    39181 acacagggtt attgccaata gaacgtcaat tctatatcag atctgatacg cgtgcagaat
    39241 tatatgcaac acttttttcc attcctttct caagtacttg gatattctca ccagttctcg
    39301 gtattcatag tggagttcag gcacttttcc ctcaaccatg gaattcgcta aaagttacaa
    39361 aagactgggt atatattgat ggcacattca atatgagagg ttggaaatct ttatacaacg
    39421 gacacggaat ccttgtatgg gaaaactggc tagagctaca tcttcctcta gttcctcaga
    39481 tcctaagctt agacggattt tttgacgctg gagcaatgat gactgagaac ggttggcttg
    39541 atatgacttt tgattcacca gtctctacaa atagtgatac tttaggatgg aataattttg
    39601 ccttctctgt tggctttggg gctagattta taattcctca gtttcctttt agattctatc
    39661 ttgctaagcg ttttgtatat gatggttcta aagtcgaatg gaagactaaa ggaggttcct
    39721 tcgattttgt tttgagcatt acgcaaccgc tctattgatt cataaagaat tcatatggag
    39781 gtctatacaa taaggctgct tcttgtacta tgaaaagcag ctctctataa tgtcttcatg
    39841 tcttctaaat cagactcgat gctcgatcaa tatcgcagaa taaaggctaa gcatcgcgat
    39901 gaaattctct tttttagatt gggcgatttt tacgaaatgt tcaatgaaga cgctcaagaa
    39961 gcgagtgcaa ttctcgatct gactttaacc aagcggcaag gccttcctat gtgtggaata
    40021 ccttatcatg ctgccaaagc ctatattgct cgacttctta aggctggtaa aaaggtagca
    40081 atttgcgaac aaaaggcact ttctaataat aagcgcggtc ttatggatcg cgaagttgta
    40141 gaagtgatta caccaggtac cgcattagaa gatgattatc tagaacaggg tacaaataac
    40201 tatttagcag acatctgtat aattgatcaa agcctttgtt ttgcttactt agatgtatcc
    40261 acaggcgaat ttagagcttt ttctttggcc ttcaagagag aaatggtagc agagacaata
    40321 agagccgaat tatatcgttt gtcacctcgt gagattttag ttcagcaatc tattcttgat
    40381 caacccaagc tttttcaggt tatacgtgag tgtggctcaa tcatagaagc ccagccggac
    40441 tgggtctatg atatttcgca ctcactcgac ttgcttaaga aaaaattcgg cattgtatct
    40501 ctaaagggtt tcggttttga ggataatgct cctgaaatcg cagccgcagg taatttactt
    40561 gaatatgtac aggaaatgat acgtaaaaat tgccctcata ttcgagcctt gctcccctat
    40621 gaagaaaaag aatacgtgct tttagatgaa gccacaaaga gaaacttgga acttgtaaag
    40681 aatcttagtg attctagcaa gagagatact cttattgagg taatagataa aaccaaaaca
    40741 gcgggcggag ctcgtcttct gcgccaatgg ctctttcaac ctctcagaat tcgcgagaaa
    40801 atcgaagaac gcttagaagc aatttcgttt ttataccata atcaaatatt actgacagat
    40861 ataagaaaat tactttatgg cattctcgat atagagagat tgatatctcg tcttgctgtc
    40921 gataaggctc atgctagaga tctaagagct attcatgatt caatcaatat tggaataaac
    40981 ctttttgctc tgatagcatc tgctcaagct cctttcgaac taacttcaag gatgagcaag
    41041 gagcaacttg aagcttgtaa gcatgttatc gatactatag aaaaaactat cttggaagag
    41101 ccatctattt tgttgacaga aggcaatatt ataagggatg gttacgacaa agagcttgac
    41161 gatctgagaa atctgcataa gaacactcag aaagtacttg aagactacct taaagatgag
    41221 aaaaagcgta cagggattca aaatctacgg ataagatata atagaataat tggttattac
    41281 attgaagtga caaaaggaaa tcttgcgaat gttccttctc atttcattcg cagacaatct
    41341 ctagttggcg cagaacgata tactaccgat cgattagcag aactagagag caagatcaat
    41401 ggagcgcagg agcgcattat tgatatcgag aagaatattt ttatcgagat aagagatagc
    41461 ctgaaacaat ttatccctgc gctgctagaa tgcgcctcag aaatatcaag gatagactgc
    41521 ttggcatcac ttgctcagac tgcaactgag caaggtttta caaaacctca aattgaagaa
    41581 gaagaagata tagagataat aaatggccga catcctgtag tagaaatctg tttgccttat
    41641 ggagattttg taccaaattc aattagcctt tcaagaaaga gcaactggtt tatattgata
    41701 acaggtccta atatggctgg taaatctact gtgcttcggc aaacggcgct tattgtgctt
    41761 cttgctcaca tgggcagttt tgtaccagct gaatcggcaa agatagggct cgtagacaag
    41821 atttactgtc gtgtaggagc gcaggacaat ctagctagag gtgagagcac ttttctggtt
    41881 gaaatgcatg agaccgcttt tatactcaac accgcgactg agaaaagcct tgtgattatg
    41941 gatgaagtag gtaggggaac agggactctg gatggagtat cgatagcttg ggcggtaagt
    42001 gagtacctta taaagagaat cagctgcaga acactttttg caacgcatta tcatgagctt
    42061 acaactatgg caattccagg gatgcgcaac atgagaatgg ttgcaaccga acaagatggc
    42121 aatatatctt ttccaaaaag gctcgaagat ggtgcttcaa gcggttctta tggcatacat
    42181 gttgcaaatc ttgctggatt gccaaaagag gttatcataa gagcttctga agttgaatca
    42241 cattttaatt ctatggagca agcattaaaa gaagaaaaac ccgaagaaac cgcagttcct
    42301 tcttatgaac tatttagcgc tgaagatatt gtcatcgaaa cattgaaaaa catagatcca
    42361 aacaatatta caccaattaa agctcttcaa attttatctg acctcgttga gagattgaag
    42421 caatgatatg aataatgagc gattatttaa tgatggattc tatcttcagg aaagctaatg
    42481 ctcagactct tgattatata tttccccagc actgtgttct atgtggatcg attgtgagcg
    42541 cagtatcttg gtctcctgca cctctctgcg cttcttgtga acggacctta gagtttatca
    42601 agggtccacg ctgcaaacac tgtggaagag agcttatcag cgagctggaa tattgtatgg
    42661 attgccgcaa tttacagact tccttgacac atataattcc aatattcagt tttcgtggca
    42721 atgcagccag tttaatccat gcatttaaga cgggtcagcg tatttctttg gcccgctatt
    42781 ttgcttatcg tatttctatg cttgatgaat tggtaggaaa tatttctact tctatatgtc
    42841 ttgttcctgt gccaccaagg cctgaaaaat tgaaattagg acaattcgat caagtaggca
    42901 ccatagcaga atcacttata gaatatggct accattataa aaagatgctt ttgagattat
    42961 caggaagcag tcaacaaaaa ttgctagatc gtgctagtcg ccttaagaat gcttcgctat
    43021 cttatacact cttatctgaa aaaaaaacca tggaaagaat cattttgctc gatgatgttt
    43081 gtaccacagg tgcaactctt gaagcttgtg caaggttatt gatgcaaaat gggaatgaag
    43141 ttgccggtgc aatagtcctt gctgctgatt aggcttaata tgcagaagcg aatcttgacg
    43201 aataatgcaa gaatatctat aatagcaata taataaagtc tgagattctt ttttgcggca
    43261 tgcaggaaga gtcgtgcaaa cggctctttt tttattccct ctatatagcg ggaaatagag
    43321 cggctactat tgaacattta gcaaatgttt tgcagctagc tgacctgcct agcagaaaat
    43381 ccgcccaaag cggtttctct gtggatatgc gcttccgctt gagcttaaca atcgtcgaca
    43441 tagaagatag aagcacatcg ccaaaagaag ataggacaaa taggccagcg cggcttaagt
    43501 ctgaatgata tttaagccga gctttatacc aaagaataga ggcgaatgga agggaatgac
    43561 agataaaaaa cttcaagaag agatcgatgc aacactaaaa gcaggagggc tcgatctatt
    43621 gga
//
LOCUS       DAOSOB010000009        43264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_926_length_43264_cov_68.601356, whole genome shotgun sequence.
ACCESSION   DAOSOB010000009 DAOSOB010000000
VERSION     DAOSOB010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 43264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43264
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_926_length_43264_cov_68.601356"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..985)
                     /locus_tag="PLS88_01865"
     CDS             complement(53..985)
                     /locus_tag="PLS88_01865"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06134.1"
                     /translation="MKTLKQFNSAIILNRSIAPNLMHLAFEWPKDLQPPLPGQFFTFL
                     PSMVESSAGIILRRPLAFAGFEPKKNTANSSASLSAIAHSIYQIRGPCTKALALQQAG
                     SYIDIIASLGNSFPYPDREEKVIIAGGGIGIGPMLFLAASLAENIAIPEDHIENILKP
                     LESSRSSKEMKLIFGFRSMPLVPSFKEATGNNSIAKTWERLLSGAFIATDDGSKGFHG
                     TVLDALEFLKKTDKLARFWHVYGCGPEAMIAKLAAFAKRNNFPAHLSAEQWMACGIGA
                     CQGCVLPARTGGYLRVCSDGPVFEANAIDWEACL"
     gene            complement(1054..1284)
                     /locus_tag="PLS88_01870"
     CDS             complement(1054..1284)
                     /locus_tag="PLS88_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HOU06135.1"
                     /translation="MSVTIYTTPSCGYCRMAKEWFRQNGISFTEYNVAADMRSAEEMF
                     RKSGQMGVPVIDIDGRIIVGFNKTEIEKSLHH"
     gene            1429..2958
                     /locus_tag="PLS88_01875"
     CDS             1429..2958
                     /locus_tag="PLS88_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06136.1"
                     /translation="MKTAKVIVTLTLHFLCCSMLAWTAETSGERLSGNLSFSASSKKA
                     KISLFLKYSSDAFINLSIPLSIDKEWLENLAFTGKTKLVGYYIDAGKLAVKDSASFLI
                     APVLVSAKGKSTLLLGDIGKSSDPIGISISSSRMSLLAGKGDPYAFAAMQYLCAQEAL
                     LVSIGVGTLLDTENSIGILARLNPWISIGIGYTASSISFMIRSHIYPDYQNKSENSFD
                     IAWLRGIGTRIDMSISAKSQKLQGFFYIETGNFLSALGKVANYDAMAQIDYEADISFM
                     RILKSLNVKLSAFSKQGLVEPLDARTPSFGTLPDLWTMKYWPDGGKIGLIIESQKAAF
                     CLFQTDLNIKTNISGALAINSISWSNKIALDLVLQRAGSSIEMLKINGILQGSHSIGQ
                     KLGEIEEETDSESIGNEESGYFEQIEEQYTSSNIGSSSSPAFDRLLLSCRLKISPFSF
                     LTSVNLPIYNMDSRAITGKIGMSLVDHRLHMELSVSMQFNPESRQLQFKSLNCYAKIP
                     L"
     gene            3078..3704
                     /gene="pncA"
                     /locus_tag="PLS88_01880"
     CDS             3078..3704
                     /gene="pncA"
                     /locus_tag="PLS88_01880"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF008623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HOU06137.1"
                     /translation="MDDALIVVDIQNDFCPGGVLAVPEGDSIIPKVNELLAAFPLSIL
                     TQDWHPIMHCSFASSKGLPPFSLDMETSPPSVLWPDHCVAGSQGADFHRALQSWRARY
                     IFRKGTRKELDSYSAFLENDGVTSTGLEGLLSSLGINRVIVCGLATDYCVKATALDAR
                     KLGFEVVVVEDAIKGIGAAPDDIDNAKKQIREAGCVFAQSGDLVSSTL"
     gene            3741..5180
                     /locus_tag="PLS88_01885"
     CDS             3741..5180
                     /locus_tag="PLS88_01885"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOU06138.1"
                     /translation="MYKNALFTDFYELTMAQGYWKRQMTMPVVFDYFFRKHPFNGGYS
                     VFAGLATLIEALEEFSFSQDDLEYLSTLGIFEDEFLHFLSSFRFRGTIYAAREGEIIF
                     PQEPVVRIEADLISAQIIEGLVLNVLNFQTLIATKTARIWNASHRGSIMEFGLRRAQG
                     ADGALSASRAAFIGGAMGTSNTLAGKEYGISVLGTMAHSWVMSYPSELDAFNAYAEIY
                     PQNTTFLIDTYNSLESGIINAIEVGKKLKEKGYSFGVRLDSGDIDYLSRAIRARLDEA
                     GFMDAKIVVSNELDEEIIESLVDDGAPIDIWGVGTNLVTGGNEAAFAGVYKLAAIDSE
                     DRLRPVMKFSDNPEKSTNPGIKNLWRLYDENGAARLDLISCQDEKIQEGLEYIVHHPS
                     ADWRRLKITPARVEHLLFKVMDRGNRTETLPNIKQIQAFMKERIQTFDSTYLRLLNPH
                     IYKVSITDRLYDLKVYLINSFLKHKLSRN"
     gene            complement(5187..5813)
                     /locus_tag="PLS88_01890"
     CDS             complement(5187..5813)
                     /locus_tag="PLS88_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOU06139.1"
                     /translation="MNISQESVALQPLNLCVFCGSSTGARPSYSEAAEELGRILAERG
                     MVLVYGGGDVGLMGIVAEAAIKNGGKVVGIIPQRLYELVDQQELSELLVVNDMHERKA
                     LMEKKADAFLCMPGGIGTMEELFEVWSWRYIGYHQKPVALLNIEHYYDHLLEMLKHMV
                     KEGFLHKEIYEDLIVSDNIAYSLDALCKKTGTSGAAPFLKKPERRNHN"
     gene            complement(5878..6234)
                     /gene="ssrA"
                     /locus_tag="PLS88_01895"
     tmRNA           complement(5878..6234)
                     /gene="ssrA"
                     /locus_tag="PLS88_01895"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(6340..7455)
                     /gene="mnmA"
                     /locus_tag="PLS88_01900"
     CDS             complement(6340..7455)
                     /gene="mnmA"
                     /locus_tag="PLS88_01900"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOU06140.1"
                     /translation="MNQDITVAVGLSGGVDSSLSACLLKEQGYNVIGLTMKIWKGAYK
                     IQENLKHSCFGPSEQEDIEACQRLCTSLGIPYYVIDLADEYELYVLEYFRSEYLAGRT
                     PNPCIVCNRDLKFGFLIDRARQSGLSFDMFATGHYARKSTINGITYIRVARDIDKDQS
                     YFLYGLDSARLSHIIFPLGEMTKKETRQAAHIYGLEAAEKSESQDFVGGGSYAPLFEN
                     KEPLPGDIVDLKGNVIGRHRGLPFYTIGQRRGIGISLGTEPVYVLGLDAERNRVIVGS
                     GDGLFSSGLIARSFRLQNPESVGERIACKVKIRQNHKPAEAIAEFDKAGTARIEFIIP
                     QRAVAPGQSAVLYSEEGLVLGGGIIEKALTDTGKIEE"
     gene            7584..8711
                     /locus_tag="PLS88_01905"
     CDS             7584..8711
                     /locus_tag="PLS88_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOU06141.1"
                     /translation="MQDVFIGFTSITWKMLVMYGIGGILIWLAIKKQYEPMLLLPIGF
                     GAILVNLPLSIAWEHEGIPGFLRILYEAGIANELFPILIFIAVGAMIDFGPLFRNPLM
                     IFFGAAAQFGIFATMIVATLLGFDLKTAASIGIIGAADGPTAIYVANKFARDYLAPIS
                     VAAYSYMSLVPIIQPPVIRLLTTKQERSIHMPLHEKQSPQWLKILFPILVTMTSGIVA
                     PISVPLVGSLMFGNLIRESGVVERLSQVAQNELAYLVTLLLGISIGGSMSAEKFLNFD
                     TLLILAMGLVAFIFDTAGGVLFAKLVNMFLPKEKKINPMIGACGISAFPMSARVIQKM
                     ANDEERGNIILMQAIGANVSGQLGSIIAGGLVLALVPLIVG"
     gene            8722..8868
                     /locus_tag="PLS88_01910"
     CDS             8722..8868
                     /locus_tag="PLS88_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06142.1"
                     /translation="MAFIDNLRNAGTAMQAIAVSIGGLLGVFTTLAFFFLMILAADRF
                     SKHK"
     gene            complement(8936..10150)
                     /locus_tag="PLS88_01915"
     CDS             complement(8936..10150)
                     /locus_tag="PLS88_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOU06143.1"
                     /translation="MKVHPINDHIFALHADIRSDDLFEGIWPIPYGVSLNCYLVKADK
                     IALIDLVRDWVGAPNELKSQLASLGVALEEIDYIILNHLEPDHTGWLREFLSINKKAI
                     VLTTAKGAELVKNFYFEHDRVQAVKEGDILDLGLGQALQFFETPNVHWPETMMTWAKE
                     GGVLFSCDGFGSYGALGDRIFDDEISEDEHEFFEAESLRYYANIVASFGIFVKRAIEK
                     LSSLPIRVIAPSHGIIWRANPNKIIERYLRYADYSDGPREKEIAIIWGSMYGNTELGL
                     KHVIEGIEEEKVPYSIHRVPDENVSFVLADAYKSEGILIAMPTYEYKMFPPMAYILDI
                     FERKHLYKRKSLRIGSYGWVGGAKKEFEAKIESLKWSCIEPVEWPGAPDEATCELLKD
                     KGRELAKWVKAV"
     gene            complement(10212..11660)
                     /locus_tag="PLS88_01920"
     CDS             complement(10212..11660)
                     /locus_tag="PLS88_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06144.1"
                     /translation="MSSLQRLEAIGILCLIIMAAPFSISSQEDSNDIDALFEDEIVVQ
                     QDRETSSQKESQDSLNRQTNPLQSFLKTETVKIGGSISGTVQSSWQWNDPWTAGFDLV
                     DPDSQKLKPDLNSLVYFDARPQEDFRVHGSFKTAWPFSETKSLLDSNNKTTLVSVPDI
                     RIFELFSDFQLGDTAYMRFGKATVKWGVGYFFSPADIINLESINILDPTAQREGPLQF
                     RVFIPYGPSQNTLSFYTIFDTDNPDFENTALAAKAEFLLGNYELGLSAYYRYDTTERA
                     ALTLTGPIWNLDVFAEGVIARGSPKTFYKSFQSSYPYYNEISSDDIRNTLYPSATIGF
                     LYNNPNSNFTAIAEYYFNGEGYSDADRAALFNSYESQQAGVKNLILYSIATGNLKSFP
                     FLSGQHYGAASISFSEIGGSDFSISALGIMNFSDISGFAQPTVSWEITDYLKLSIFST
                     FIFGADSTEYGFLGRGRPVTIGIKLSAGTGNF"
     gene            complement(11697..12494)
                     /locus_tag="PLS88_01925"
     CDS             complement(11697..12494)
                     /locus_tag="PLS88_01925"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOU06145.1"
                     /translation="MHSNVFLKTRGLKKESYFIIILAMLFFVGLNPLLAQSKPDPKAL
                     IKQVDDLSDFSGKDFSAVFTIVTQKPGEKDSVTQARIFRRDLKKQFVILILLPEVNKG
                     QGYLREDDNVWFYDPTSRKFSHSSMKENLQNTKAKNSDFTLSTFAEDYTVTKMTDGMV
                     GKFPVWILDLKAKTNEVAYDRVVLYIRKDRTMLLKREDYSVNGRLMRTTAYPKYVELE
                     GRLLPSQILIVDEVNKGEKSQITMAEQSVSPLPDRVFTKAFLEQVSR"
     gene            complement(12510..13835)
                     /locus_tag="PLS88_01930"
     CDS             complement(12510..13835)
                     /locus_tag="PLS88_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU06146.1"
                     /translation="MNVVLLRIAARNLMRQRKRTMLLAGAIAFGIMIVTIINGFAGSF
                     VKNISDNFSYLMGGHVFVSGSEYTPSGKRISIIRDDSVIMQALEDSGQKWVSLQKTSQ
                     TIATLIFEGKSMQQTLTGIDLSHSGMIKERLALVDGNWDNISWPNAIIISQSIAKKLN
                     VLPGDVLVAQLQTITGQNNVGEFRVAAITIDSSFVGSMMTYVQLEYLNSLIGLGKNEY
                     MSLGIMLERLEKSGPFANALYTSMKGMGLNLFDRSTKKEDGSMTPFQAMLLEQKTEQW
                     TGTKYRLYTIDDVLAQAQQIVVALDTGSTVVLIVLFAIVMIGISNTFRMAMYERIREI
                     GTMRAIGVQRNEIRSMFLYEALLLALAGAIAGIILALIAMGLLTFISFDPLSPVFLIL
                     KRGHFSFYVPPLRAIGNILLIAVLTLVAVYAPANAAAKLPPAEALRTVK"
     gene            complement(13846..15348)
                     /locus_tag="PLS88_01935"
     CDS             complement(13846..15348)
                     /locus_tag="PLS88_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU06147.1"
                     /translation="MAVIFKIAFRNIKAHRSKSLIIGILLAMGAMVLVFGTAFINATE
                     EGVRSTFSDVYTGDIFISGISSDGPVSLFGVTSTSGMAETPIIPEYDKVLSIVKSTPH
                     LKKTSSLATGFSQVIRENGDQMTKEDSQNKIKPQDRFLFLFGIDAATYWDLFETVEIV
                     AGEKLKPGQSGIMINESQFYKFSDWFEKPLSIGDPLLVQGFSTIGFRLRELPIVGIFR
                     LKEQGTAPEQLAYIDIESLRVMSGMTVGANEPIHLKPEETSMLSIEDPDELFSDDIIV
                     TSETSEHFDVSSISRELSKPSQIDPLKPDEGAWQFIVGKADSPQNINRIISSLNKAFE
                     KEGIQAVAGSWQAAAGPYGQSVDVVRLIFAVAIIILSIVAIIIIMNTFVISVIERTSE
                     IGTMRAIGAGKGFVRTMFAAEAMALALVSASIGAGLGLLIIEILKSLRIEATNPFFLV
                     LFGGQYLNPIATAGNVFFAIVLMIAVGFIAHLYPVSLALKIQPVRAMQEE"
     gene            complement(15351..16088)
                     /locus_tag="PLS88_01940"
     CDS             complement(15351..16088)
                     /locus_tag="PLS88_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06148.1"
                     /translation="MPIIQVIDVKKDYYLGNVVVPALRGISFSIERGDFVSIAGPSGS
                     GKSTILNIIGLIDVPTSGELIIDGKNTKGLSDKELTRFRHEILGFIFQSFNLIPVLNV
                     YENIEFPLLLGKTQVPKSEWKSWIEFLIGEVGLTEWRNHKPNELSGGQRQRVAIARAL
                     VTKPSIVLADEPTANLDSTTGEQIIDLMKKINRELETTFIFSTHDAKIVNIADHVIRL
                     RDGMVIENRRSGEDIFEENAFTASAEN"
     gene            complement(16299..17546)
                     /locus_tag="PLS88_01945"
     CDS             complement(16299..17546)
                     /locus_tag="PLS88_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOU06149.1"
                     /translation="MQDLSVYLSANAHSMKKSVIRELLKLTNQPDIISFAGGLPAPET
                     FPIEELRVASDQVFRKYGDKILQYGTTEGDNDLKARIIEYESSHGIDLDPDNILITSA
                     SQQALDMVPKLFLDPGEYVIAERPTYLGAIQAIQSYQGKVLGIPFSHANDGLDMDELE
                     RKYARAKSENKRIKYIYVIPDFQNPTGVCWSLEKRKALIEFAYREGLFIVEDAPYREL
                     RFMGEPIPSLYQLELEMQNLGIVIGLKTFSKILAPGTRIGWILARKDIIQQLVVAKQA
                     VDLCTSPLTQKIVAQFMATGKLEEVLKNTCAIYRDKRNFMLQKLDTYMPKHPDLTWTK
                     PEGGLFLWLSLPSYIDTDKMFYKAIEKKVAYVVGSSFYYDDPEVNSMRINFSYSSYQQ
                     IEEGVKRLASTFKEEIEAHEAGL"
     gene            17732..18838
                     /gene="ychF"
                     /locus_tag="PLS88_01950"
     CDS             17732..18838
                     /gene="ychF"
                     /locus_tag="PLS88_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOU06150.1"
                     /translation="MPLNCGIVGLPNVGKSTIFSALTRAKVDAANYPFCTIDPNVGIV
                     PLPDKRLDKLFEIFKPKRKVPAVVEFVDIAGLVRGASKGEGLGNQFLSHIREVGMIAH
                     VVRCFDDPNVVHVAGKINPADDIEVIHTELALADLESIDRRLDKLNRQVKVQDPNLKK
                     DIEKEIAVLQKLRPALEEGKPASTINLESEELDYARRSFLLTLKKELFVCNVDESGID
                     NNKYVEIVKSIAEKQGSEAVVICGKYEAELADIEDSEERAEFLHSIGLEEPGLDILAH
                     AAYRLLGLRTFFTTGEDECRAWTIKVGDKAPTAAGVIHSDFEKGFIKAEVYHYDDLMN
                     YGSENAVRAAGKMRQEGRDYVVQDGDIIFFKFNV"
     gene            18913..21735
                     /gene="uvrA"
                     /locus_tag="PLS88_01955"
     CDS             18913..21735
                     /gene="uvrA"
                     /locus_tag="PLS88_01955"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOU06151.1"
                     /translation="MDKLIIKGAREHNLKNIDLELPRNRLIIISGLSGSGKSSLAFDT
                     IFAEGQRRYVESLSAYARQFLGRMDKPDVDYIEGLSPAISIEQKTTTRNPRSTVGTVT
                     EVYDYLRLLFARIGVPHCPSCGREIREQSVDQILDVILSWPMDTKIQVLAPVIRAQKG
                     EHKKILEDALKQGFLRARIDGQFIELESPPALEKQRKHTIELVVDRIRLSEESRRRVA
                     EAVETSLGIANGIVTIMREIDGQQKEEFFSQKGACPICGISLPEMEPRLFSFNAPQGA
                     CPACSGLGMNMEFDPGLIIPDKSLSFNEGGCAPYNPNSAWNRSKFEALADHYHFSLDT
                     PFDKLPSKVIEAILYGSDDNIHVRYNNRDKTGYFEYESRFPGILADLRRRYMESTSND
                     IKDWLESFMIEKECEVCQGKRLRPEALAVTIGGKNIYEISCMPVEESVQFFKSLKLTN
                     TETQISKLILKEIIARLGFLQNVGLEYLTLERQASTLSGGEAQRIRLATQIGSSLVGV
                     LYILDEPSIGLHQRDNQRLIDTLTYLRDLGNTLIVVEHDEQMLRTADYIVDLGPGAGE
                     HGGYVVAQGSVQDVAANPQSITGQYLAGSLKIEVPAVRRKGNGACIALKGASEHNLKN
                     IDVRIPLGRFVCITGVSGSGKSTLLSDVLYPAISNRVMRSSIREGSYKSLEGIEYIDK
                     IINIDQSPIGRTPRSNPATYVGVFTPIRELFASLPESKARGWKPGRFSFNVKGGRCEH
                     CQGDGTIKIEMNFLPDVYITCEVCHGKRFNSDTLDVRFKGKNIADVLNMTVEEASEFF
                     AYIPSIAHKLQTMLDVGLGYIRLGQSALTLSGGEAQRVKLSLELARRATGRTLYFLDE
                     PTTGLHFADVRQLLEVIRRLVDSGNTVVMIEHNLDVIKQADWIIDLGPEGGERGGMVI
                     AEGTPEEIALCRVSYTGQYLRRLLNI"
     gene            21792..24767
                     /locus_tag="PLS88_01960"
     CDS             21792..24767
                     /locus_tag="PLS88_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06152.1"
                     /translation="MQRKIGLLFLIQLLLVPLAAQAQIQIDEGTSKPEDVGSKSDNQI
                     TLTIEQAVSAEYFSLENGTKELRLTGPLVIQLIDSSGVTHTIKAQYVVYNRDTKEVKA
                     IGSVEYTRQTEYKTDIYRGESIAVDLDDNSGVFIDGSFNMESTASSSRKLIVHFESLI
                     SRSEEVISLANGSLTACDEIKPHYILRAKKIWLFESGDWAASNAILYVGAIPVMWLPL
                     FYYPSKASKFHPVVGFRSRAGGFLQTTTYLAGAQSSEAQKSSMLSTTEGALGSFGTYI
                     SRTQPSSESKNQTNIAILGDVYSSLGVFAGLRGELASTYSYDLSWLVGAAFSRSVFLE
                     STGYYSPYDSAGDYASVWNKWKLASIELPMRILLNFEASSKKNKTGLKWQISLPFYSD
                     PFVEQDFMYRNESYDFFSILGNSSLKTIDERSSFSQKASLSWSWNPSIEKSNFTFRLS
                     NLSSSFNWKSKYASTTGMNSQELHLNAVDPQRHFFYPDSARIIDNAFTMSGNILQSKN
                     MSLKWTSTNSGFIEDRFYSSQWQKPQDIDFGSWYWLYGGRSNADLTSSYSIDDAGLTF
                     QVSTGVLGQLQYRPYLYDERTNPTTVHPFLIADYGYNTAYWSTGTKLLWEPFKKSNIF
                     SASKFSYSIQGKVARLAYEGLDGSGIDAYPIYKLYWLSWDPTMISDHSMLTELSAKLG
                     STTERLSFKVALPPLLESYTLSGSTALGLATMGASYVISRKDSLEDLKSTSLTGNLTL
                     KPTKKIGISANAAWDFDTQAPLSLSTELSAWSFNARFVAQKASGYIFKGSSWIQDGTQ
                     YFRPSTLSLSWKPTMRSDVPLFSKDTIGWSVEANGLLTLNQNLIQSTNSTFGANLGFS
                     IKNSTGLSFNFTLSSVNSSFWRYYASLLPKSDDFDPEHYSKNFFLDLWDSMSIWDMNK
                     LKNTLFKLQSLKVGLSMDAHDWVLSANIEAGPELVTASSTQPLPHYEMEVAFNLAVTW
                     KDISAIKGSINYSEGTFKR"
     gene            24936..25928
                     /locus_tag="PLS88_01965"
     CDS             24936..25928
                     /locus_tag="PLS88_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOU06153.1"
                     /translation="MSPSHIVLEDTQVFAELCGANDYNLSLIGSLLGTHVLTRGNELF
                     VESDDPAVLSLFSRLISAIGTSIEEGVPATPELIIALHAELTPDGSQSLDASIENPAE
                     EKESDISRFLDNCIQIPNGFGKVFPRSAGQSLYLQGIASHDLTFSIGPAGTGKTYLAM
                     AYALRELLTRSKRKLILTRPVVEAGESLGFLPGDLEQKINPYLRPLYDAMEALVPYET
                     IRRLEESRAVEVAPLAYMRGRSLANCIVILDEGQNTTKEQMKMFLTRLGEHTKAIVTG
                     DITQIDLPKKTESGLIHAITLLAHIDEVFISRLDARDVVRSSLARKIIEAYEKE"
     gene            25915..28116
                     /locus_tag="PLS88_01970"
     CDS             25915..28116
                     /locus_tag="PLS88_01970"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU06154.1"
                     /translation="MKKNSQESWFFELKKSLRSVQPPLWLFFAAIVIAAIGLILVGPS
                     SISSRSSLKAIKVGEIAETDIYAGKDTIYIDKEATQRKIQAEERLVLAVFVMDDKITA
                     SIREKAEEFSSYYLQLLGSKSGSINALSLMLSSRFPDLLQSDQYEHLVRAELNAQTFV
                     YVSDIVESLLSKGIVSVPEGVFDRFNPDYYELVRILNGREESEKIAVSSMITLVNLGN
                     RLEDEMDARHIPESLKQYVRFLGKALLRENAFFDQNLSEKRLAAARARVEPVTRFVSR
                     NEILVRKGELITPEVYQQIKTIRSSILVSDIGVLLTGLGLLLIASLLGLLLSRMPDLS
                     DFPMDRSSLIFLLISFLILFYWILVIQNLLSGKSFSHGALFVPASLFAGLVSLLYGAR
                     TGIFFSLVSFFLTGAATNLNAQFMIGILLAGISATLIMRTAHTRIMLARAAVLQALVQ
                     AMLAMILLLNQKPSAAEVLQAMGLGALNGFAGGSFILLLLPLFERLLDRSTQFRLMEL
                     ADTNAPILKEMLMKAAGTYAHSMNVANLAEAAAKDIGANPLLARIGAYYHDIGKIDHP
                     EYFAENQQGINLHDDINPTLSASIIRRHVKDGIERAREIGLPKEVIEIIGQHHGNSVM
                     EAILVKAKDAAAQVDVSSYSYQGERPRSKEAGIVMLADSIEAASRSLKNPNASKLEDF
                     VHQLILKKLTNGQLSDSELTFNDIEVAEKAFLRILQSQMHTRLEYPEQRKQ"
     gene            28113..28574
                     /gene="ybeY"
                     /locus_tag="PLS88_01975"
     CDS             28113..28574
                     /gene="ybeY"
                     /locus_tag="PLS88_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOU06155.1"
                     /translation="MNTNEVFYSMIGIDEPSWAQRSADYALSVLDALGKKDWQISLTF
                     CDDNTIQTLNRDYRHVDSPTDVLSFVLGEYEPIEGSESIYLAGDIVISIPAIERNAEE
                     YGVGEDEELRRLIIHGILHLTGMSHEDDSPDQPMLLLQEQLLKTLGGNVIL"
     gene            28571..29371
                     /locus_tag="PLS88_01980"
     CDS             28571..29371
                     /locus_tag="PLS88_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU06156.1"
                     /translation="MNILSQLFGVHGKTVESKDEVVEEQREMIEGVEHLPETIVRDVM
                     VPRTDTVCVEDISPIDEILETLVDSGHSRIPVYSGTIDNIVGILYAKDVLAALVKKKP
                     LEVKSLMRLPYFVPETKRIDTLLKEFKRRHVHIAIAVDEYGGTAGIISLEDIIEEIVG
                     EIQDEFDEEADQVVPIGKNRWSCDARIHLEDLNDAIGAELPYEEYDSLAGYVFDLFER
                     IPSKGEQVTVGDLQFTVEEMEGHRLTKICIEKLDMSQEDSPFTPMHVS"
     gene            29491..30837
                     /gene="gdhA"
                     /locus_tag="PLS88_01985"
     CDS             29491..30837
                     /gene="gdhA"
                     /locus_tag="PLS88_01985"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOU06157.1"
                     /translation="MNSYIKEVLDGLRARYPWESEFLQATEEVLESITPLIEAEPKYR
                     QQKILERIVEPERVVIFRVPWVDDKGEYHVNRGYRVQFNSAIGPYKGGIRFHPKVTLS
                     TLKFLGFEQVFKNSLTGLPMGGGKGGSDFDPNGKSDNEIMRFCQSFITELFRYIGADT
                     DVPAGDIGVGGREVGYMFGQYKRIANEFTGVFTGKGLTFGGSLVRPEATGFGAVYFAE
                     EMLKTMGDTIEGKTVAISGFGNVAWGACMKASQRGAKVLTISGPDGYIYDPDGVGTKE
                     KWDYLGEMLIIDRNKVENYVRKFPNAVFHKGKKPWEQKVDLAMPCAIQNEMNGEDARM
                     LVENGVKVVVEVSNMGCMPEAWKYFIEKKIPFGPGKAANAGGVATSGLEMSQNSMRLS
                     WSAEEVDRRLHEIMANIHKSCVETAAKYGFEGNYVVGANIAGFKKVADAMIAQGLV"
     gene            30863..32203
                     /gene="aroA"
                     /locus_tag="PLS88_01990"
     CDS             30863..32203
                     /gene="aroA"
                     /locus_tag="PLS88_01990"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOU06158.1"
                     /translation="MGTMVAKPGRVSGSIQAPASKSALQRAIACAILARGISHIEASP
                     ICSDARAAIFIAKELGARVQEEKGILEIEGSALFHKDTEVESTILSKKVSYPSLQDQR
                     FQSYSPLILPCGESGLCMRMFSPIVALLDKEVELQGEGSLSSRPMNMVEESLKSLGIF
                     CVSRGGVQPIRIRGPLRPLEGRVNALGSSQFITGLLIALPLLDGDSELIVDNLVSSGY
                     LDLTIEMCAEFGVEIKREANGSRFLVPGYQVYKAADLAIEGDWSAGAFLAVSAAISGR
                     EDGLRIQGLRQESTQPDRAIVDVLTKAGAQVQFEGSDCIVRAANLVPFDFDATDCPDL
                     FPPLVAMASAIHGRSCIRGTRRLLSKESDRSAALCKTFRKLGAEVTLQDDAMYIEGGS
                     IHAATIESFGDHRIAMAASIAALVSSGEVYIEGAECVSKSWPGFFEDLSSILVS"
     gene            complement(32292..33068)
                     /locus_tag="PLS88_01995"
     CDS             complement(32292..33068)
                     /locus_tag="PLS88_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOU06159.1"
                     /translation="MRIAILGAGRMGAWLTEELCWNNEVMVHDSDLLKMKYFIKVHRA
                     LSIEEFADFKPELFINCVPLGLTLEAFDTVLPYLPSQCIISDIASVKTGFKEYYENCG
                     HPFVSSHPMFGPTLANIRDLHEESAIIISESSKEGADFFRRFYSSLKICIFEYSFEEH
                     DKTVAYSLATPFASTMVFAACMKKQDAPGTNFKRHLAVAKGLLSEDDRLLTEIMFNPY
                     TIRQLELINSKLAYMTHIIRQRDYDEMKKFLDKLRANIGQ"
     gene            complement(33065..34123)
                     /locus_tag="PLS88_02000"
     CDS             complement(33065..34123)
                     /locus_tag="PLS88_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOU06160.1"
                     /translation="MNLDDIRSDIDRIDAKILSLLNERMEKSLLARRFKATAFDPVRE
                     QIVQEKVRRSSQCLLDPHFSVHLYEQIIAECRRLQDAGLKTVAFQGEHGAYSEVALRL
                     LMPEAATIPCLEFSDVFDGVEKGIYDYGIVPVENTLGGIVGPVNSILIYTHLKIVAAI
                     DLPIRHCLLTIPGADHRELRIARSHTQALTQCRNFLLRNHLDPEPFYDTAGAAKALAE
                     NRPKGVSVVASKFASDLYGLEIIKEDIQDSPHNRTRFFIIATEDNGVEGDKCSAVFTA
                     GNKAGALFSVLEVFAEENINLTRIESVPDVPEKYAIFIDFEGSLSSAPVQRALEKVPK
                     IAEGFKILGCYHEMRVES"
     gene            complement(34120..35142)
                     /locus_tag="PLS88_02005"
     CDS             complement(34120..35142)
                     /locus_tag="PLS88_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOU06161.1"
                     /translation="MNTVGRIFRVSLYGESHGKAVGVVIDGCPAGIQLTEEDFTEDLN
                     RRKAGAEGTTARIESDKPIILSGVFDGHTSGAPIHIAFRNDNVRSSDYEQFTNIPRPG
                     HADFSAMMKYQGWQDPRGSGHFSGRISIGIVAAAVVAKKVLNTFFDIVFQTKLLEAGG
                     SRDIEKAVLRAKESGDSIGALVEIRISGVPAGWGEPFFDAAESVISHYIFAVPGVRGV
                     EFGDGFSAVAMRGSQHNDPFIDLQGRTARNGAGGINGGITNGNDIVLRIAVKPTSSIA
                     SAQHTLDFSCDEMVDLEIQGRHDACIALRSAVVLEAATACALADLALLARAEIPFACN
                     IPWRKS"
     gene            complement(35167..36225)
                     /gene="aroB"
                     /locus_tag="PLS88_02010"
     CDS             complement(35167..36225)
                     /gene="aroB"
                     /locus_tag="PLS88_02010"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOU06162.1"
                     /translation="MQRNQSIIRFGKTSELFSLIPSEKAIFVTTETLYRQSLEMFSDS
                     GLNAKEELHWIRVPDGEACKNFSVLENICRYMIDFDANPDTILVAIGGGSLSDLVGLA
                     AHLWMRGIELVLVPTTLLAMIDASIGGKNAIDIGTTKNLIGSFHLPNLILCDVRWLET
                     LPSRNLLSGMAEAIKHAAIDSEEHFLFLEEIINSGKSLTQIQAELWEELVRRSQQVKI
                     RYVDADPYDNHLRHALNYGHTFGHAIELITGIPHGFAVATGIGMANALAVSLGVLDPD
                     IAARIDLLLRNLGLPTNIKEASAIAGYNISPSTICDLIRADKKRRGESIDFALLSKIG
                     NVRIEPIAIKYVVEFLNA"
     gene            complement(36236..37165)
                     /gene="aroF"
                     /locus_tag="PLS88_02015"
     CDS             complement(36236..37165)
                     /gene="aroF"
                     /locus_tag="PLS88_02015"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOU06163.1"
                     /translation="MFKNIKLAARNPGQKTTIIDVNGVKVGDDLVIIAGPCSVETEKQ
                     TIETAIAVKKAGAQMLRGGAFKPRTSPYAFQGLGLKGLKILDKARRETGLPIVTEVVD
                     TRDVSWVGEYADVLQIGARNMQNFSLLREVGKSGKPILLKRGMYSTLEEWLNCAEYIL
                     AEGNPNVILCERGIRTFEAYTRNTLDLSMIPAVHQESHLPILVDPSHGTGILSLIEPM
                     SLAAVAAGVDGLEIEVHIDPQSALSDKDQQLTTEMFESLMEKLRGLKRFLDESNVEFI
                     GQASGECLSLSNNTTNVSDAIGTKTDAFSEAVD"
     gene            37300..40305
                     /locus_tag="PLS88_02020"
     CDS             37300..40305
                     /locus_tag="PLS88_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOU06164.1"
                     /translation="MYFNELVSIYDNQRRERNIYHDLMRDRVQEILLVGSLYDSFVVE
                     SDGVLTEQIYGEYFKLNLNTIPRITCAYTEESAIELFRDGRFDLVIIMASTDFDMPLR
                     LATSMKSIWPDIPILLMVTDNSSLAMLDMSRLELAAFNRIFVWNGYSKLFVGMIKYIE
                     DWRNVEADTQAGQVRVVLLIEDSVRYYSRYLPVLYRVVLKQTQALIEGEQITEINKLM
                     SIRARPKVLLANTYEEALKIFEKYKPYLLTVITDIRFHRNGEFDDNAGFEFLKFVKQE
                     LPDLPMLVQSSETDVREKANAFGASFIDKNSESLEMELAAFFQANLGFGNFVFRMPNG
                     AEIGRAGNMNEFTQRLNEIPIESLLYHAEHNHFSAWLMARGEIHVAKLMKPYKIADFS
                     SPEELRQFIVNMIEQIRSAHSRGIVPNFDPEMVKHPRYLFKIADGSIGGKGRGLLFIR
                     SFLDNFDFSQYVPGIEISMPNTAFIGIDEFEHFLESNGLWSWAYYDKEAQDLRSVFVQ
                     KPLSQKLENNLRQLLSVSKGPLAVRSSGLFEDMLMVPFSGVYDTYILPNNHPDGEVRL
                     RQLSMAVKLIYASLFSKEARAYFEAANYNLEEERMAVIIQELAGSQHGDYFYPHVSGV
                     AQSFNYYPISYIKPNDGLCIAALGLGTYLVTGGTAFRFCPRYPKLDIELLEHPLENTQ
                     RSFHALSLRDNNPDLLKGEMASLSELPIDVAENDRYFSMFASTWDALDQRFVPGVSAN
                     GHRIIDFANILKHEAYPFAKAIDIALDIGTRSMGTPVEIEYALNFSDNKQEPTLYLLQ
                     LKPLIHPEDHIEIDLSSINKEACFLLSRNCMGNGRDLNIHSIVWVDPRKFQRGETLEI
                     AAEIEELDSFARKENFDYLLIGPGRWGTRDRWLGVPVSFDQISHARVIVEMDLPGFVV
                     DSSLGSHFFHNLTTMHIKYMKITQGSDEDFIDLEWLYSLEPRIRTKHCVLTELKNPLD
                     MRFDGRNSKGAIFKSSEAGNLELTIKAGN"
     gene            40357..41919
                     /locus_tag="PLS88_02025"
     CDS             40357..41919
                     /locus_tag="PLS88_02025"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOU06165.1"
                     /translation="MIDQSAARTLHPLEVKLILAFDEGEIIDSQRVKQVLGFREGQEQ
                     QAFSWLLAKGVLEEAERKTEIFYELTPLGQEWHKKGIPAKRIFSLLKDRGPMSLPEIA
                     QSLGLDQKTVGSAFGSLSKEGVCTIDEARRAKLSKNEFPLSLLEIEMLLALASSQGGS
                     VSENALSNSQKTLMAQIAKKRGAQDSPFRIADRIRVFYRVTPIAKEYRNAISTLGMTG
                     EELGAITPEMLEKGTWKQGHFRPYNVQIPPARVIPGRRNAYVEFLESVKDKLVSLGFV
                     EFDGNLVETEFWNSDALFMPQFHSARDIHDVYYIEEPTHARFIEEPYLSHVAAAHENG
                     GNTGSRGWQYQFDRDFTRRLILRSQGTVLSARQLPKAEVPGKYFGIVRCFRYDRVDAT
                     HLSDFYQTEGIVLGKNVNLRTLLGFLEMFAVEVAGAKEVKYVPGYFPFTEPSIEVHIK
                     HPVLGWFELGGSGIFRPEVTSSLGVEVPVLAWGIGIDRMALMALGLNDLRDLFCTDLE
                     SVRMRRSQYRIQ"
     gene            41935..>43264
                     /gene="pheT"
                     /locus_tag="PLS88_02030"
     CDS             41935..>43264
                     /gene="pheT"
                     /locus_tag="PLS88_02030"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOU06166.1"
                     /translation="MPKIEVNEEFFFQLAEQSWSNREDLERDLTIAKAELDEWDSAPL
                     EASERTIKIELNDTNRPDLWTTAGLARQLRIYRTRNYPSYRFFTSKEKNLDASYRVVV
                     EKTVREVRPWIACFVAKGKAISDALLRDMIQTQEKLAWNYGRKRKGVSIGIYRIGLIE
                     WPVRYFGTKPDSVSFVPLQETRSMTLSQILKEHPKGIEYASILEGKPIHPLLTDSRGR
                     VLSYPPIINSADIGAVQVGDSEVFIEVTGTDYPSVALSASIMACDFYDIGFEIENVRV
                     DYEYETPFGSSVVFPYYFQKGVELSLIEANRLLGSSIDVEEAQDALARMGLSACCEDG
                     LTLHVSPPEYRNDFLHPVDVAEDLMIGLGMEKFDPERPTDFTIGRLTPIEVFSRKAKD
                     IMIGLGYQEMIYNYLGSGMDYAEKMQIPANQLVKIANPMTENYEYVRNSPI"
BASE COUNT        11973 a         9297 c         9745 g        12249 t
ORIGIN      
        1 aagtctggct cgaaagaagc agaagaatta ttttggcctt ctattgagtt cttcacaggc
       61 aagcctccca gtctatggca ttcgcctcaa aaactgggcc atcagagcaa acgcggagat
      121 aacctcccgt tcgagcagga agcacacaac cttgacaagc tcctataccg caggccatcc
      181 attgctcagc agaaaggtgg gcagggaagt tatttcgttt cgcaaaagct gccaattttg
      241 caatcatcgc ctcgggacca caaccataga catgccaaaa tctagccaat ttatcggtct
      301 tttttagaaa ctccaaagca tcgagcactg tgccatggaa acctttagat ccatcatccg
      361 ttgctataaa agctccggat aatagtctct cccaagtctt ggctattgaa ttattacctg
      421 ttgcttcttt aaatgaagga acaagaggca tacttcgaaa tccgaaaatt agcttcattt
      481 ctttactact tctggaagat tcaagcggtt ttagaatatt ctcaatatgg tcttcgggaa
      541 tagcaatatt ctccgctagc gatgcagcca gaaatagcat tggtccaatt ccgattccac
      601 cgccagcaat tatgaccttc tcttctctat cgggataagg aaaactattt cctagcgatg
      661 caatgatatc gatatagctg ccagcttgct gtagggcaag agcttttgtg catgggcctc
      721 tgatttgata tatcgagtga gcgattgcag aaagagatgc agaggaattt gccgtatttt
      781 ttttaggctc aaagccagca aaggccaaag gccttctcaa aatgattcct gcactcgatt
      841 caaccatcga aggcaaaaaa gtaaagaatt ggcctggtaa tggtggctgc agatcttttg
      901 gccattcaaa agccaaatgc atcaagtttg gagcaatgga tctatttaaa attatggccg
      961 aattgaattg cttcaaagtc ttcacctgag cgcaattcta tcagaagaat tatagatttg
     1021 ataccagacc ctaaaataat ttaagtattc cactcaatga tgcagagatt tttcaatctc
     1081 ggtcttattg aagccaacga tgattctgcc atctatgtct ataactggta cgcccatctg
     1141 acccgatttc ctgaacattt cttctgcgct tcgcatgtca gcagcgacat tgtattcggt
     1201 aaaagaaata ccattctgcc ggaaccattc ttttgccatt ctacaatagc cgcagctagg
     1261 agtcgtatag atagtaacgg acatatattt ttctccctct tcgatataat gattatgatt
     1321 catagaaaaa ataatatata ttgacattca tgtcaattat tctgaaagac aaaagcaatg
     1381 ccaaagaatt tttgaagcat aggcgccttc agctagcgtc tctattatat gaaaacagca
     1441 aaagtaattg taacattgac tttacatttt ctttgctgct cgatgctagc ttggacagcc
     1501 gaaacatcag gagaacggct gtctggaaat ctctctttta gtgcatcatc aaaaaaagca
     1561 aaaatttctc tattcctaaa atatagttct gatgctttta tcaatttatc gattcctcta
     1621 tcgatcgata aggagtggct tgaaaattta gcatttacag gcaagacaaa gcttgtaggc
     1681 tattacatag atgcaggaaa gcttgcagta aaagattcag ctagcttttt gatcgctcct
     1741 gttttggtat ctgctaaagg gaaatcaacg ctattgcttg gcgatattgg gaaatcgagc
     1801 gatccaattg gcatttcgat ttcttcttca aggatgtctc ttttagcagg aaaaggagac
     1861 ccttatgctt ttgctgcaat gcaatatcta tgtgcacaag aagcattgct tgtttcaatc
     1921 ggagttggaa cattgctcga tacagagaac tcgataggta ttttagctcg acttaatcca
     1981 tggatttcaa ttggtattgg ctataccgca tcctcgatat ctttcatgat tagatctcat
     2041 atatatccgg attatcagaa taaaagcgaa aactcattcg atattgcatg gttaagagga
     2101 atcggcacaa gaatcgacat gagtatatct gctaaaagcc aaaaactgca aggtttcttt
     2161 tatatagaga ctgggaattt tctatctgcg cttggaaagg ttgctaatta tgatgccatg
     2221 gctcagatcg actatgaagc agacatatcc tttatgcgga ttttgaagag cttgaatgta
     2281 aagctttctg cattttccaa acagggctta gtggagcctc tggatgcgag aaccccatca
     2341 tttggtacct tacctgatct atggactatg aaatattggc ctgatggtgg aaaaattgga
     2401 ttgataatag aaagtcaaaa agcagccttt tgcctatttc agacagattt gaacataaaa
     2461 accaacattt ctggggcttt agcaatcaat tctatatctt ggtccaataa aattgcttta
     2521 gacctcgtgc tgcagagagc tggttcttct atagaaatgc taaaaatcaa tggaatcctt
     2581 cagggtagtc attcgatcgg gcaaaaattg ggagaaatag aagaagaaac tgactcagaa
     2641 tctatcggca atgaagaatc aggatatttt gagcaaattg aagagcagta cacgagctcg
     2701 aatatcggtt caagctccag ccctgcattc gataggcttc ttctctcttg tcgattaaag
     2761 ataagtccgt tctcattctt aacctctgtt aatttgccta tctataatat ggattctaga
     2821 gctataactg ggaaaattgg aatgtcttta gtagaccatc ggctgcatat ggagctctcg
     2881 gtatccatgc agttcaatcc agaatctaga cagttgcagt tcaaatctct aaattgttat
     2941 gccaaaattc cgctatgagg ttattttaac gtaatatgag gttattttag cgcaaagggt
     3001 agttttcaat tttctctctc tgcgatacca ttttatagaa gactagcttt tcaatatttt
     3061 ccaagggagg aattgagatg gatgatgctc tgattgtcgt agacattcag aacgacttct
     3121 gcccgggtgg agttctggct gttccagaag gagattctat aattccaaag gtcaacgaac
     3181 tgcttgcagc atttccgctc agtattctga ctcaggattg gcatccaatt atgcactgtt
     3241 cttttgcttc ctctaaagga cttcctcctt tttcgctaga tatggaaaca agtcctccct
     3301 ctgtgctctg gcccgatcac tgcgtagcag gaagccaagg agcagatttt catcgcgcac
     3361 ttcaatcctg gagagctcgc tatatcttta ggaaaggaac acgaaaagaa ctcgacagct
     3421 attcagcctt tctcgagaat gatggagtaa catctacagg ccttgaggga cttttatctt
     3481 cgcttggaat aaatagagtt attgtatgtg gattagcgac tgattattgt gtaaaggcta
     3541 cggccttgga tgcaagaaaa ctaggatttg aggttgtagt ggttgaagat gctattaaag
     3601 ggatcggcgc agcgccagat gacatagata atgccaaaaa acagattcgt gaagcgggct
     3661 gcgtttttgc gcaatccggc gatctagtgt catcaactct ataatagtaa tcctacatcg
     3721 aaaggaatta tatgaattgc atttataaaa atgccctctt tacggatttc tatgaattga
     3781 caatggctca aggctattgg aagcgccaga tgaccatgcc tgtagtcttc gactattttt
     3841 ttcgcaaaca cccattcaat ggtggctatt ccgtatttgc gggtttggct actcttatcg
     3901 aagcgctcga ggaattctct ttttctcagg atgatctgga atacctctcg acattgggca
     3961 tcttcgagga tgaatttctt cactttctat cttcttttag atttagaggg actatatatg
     4021 ccgctcgtga gggcgaaatt atcttccctc aagagccagt tgttcgcata gaagctgatc
     4081 taatctcagc acagatcatc gaaggcttgg tgctcaatgt gctaaacttc caaactctaa
     4141 tcgctacaaa gacagcgcga atctggaacg cttcacaccg cggttcgatc atggaattcg
     4201 gtttgcgaag agctcagggg gcagatggag cgctctccgc gagtcgagct gcatttatcg
     4261 gaggcgcaat ggggacatca aacacccttg cagggaaaga gtatggaatt tcggtgctcg
     4321 gaaccatggc gcattcctgg gtcatgtctt atccgagtga gctcgatgca ttcaatgctt
     4381 atgcagaaat ttatcctcag aacacgactt ttctcatcga tacatataat tctcttgaat
     4441 ctggcataat aaatgcaatt gaggttggga aaaaactcaa agaaaaaggt tatagttttg
     4501 gggtacgcct cgattcaggg gatatcgatt acctctcgag agcaattaga gctaggctag
     4561 atgaagctgg ctttatggat gcaaaaatag ttgtatcgaa tgagctcgat gaagagataa
     4621 tcgaaagcct tgtagacgat ggtgcgccaa tcgatatctg gggtgtagga acgaatcttg
     4681 ttacaggtgg aaatgaagct gcctttgcgg gtgtctataa actcgccgca atagattcgg
     4741 aagataggct gcggccagtc atgaagttct ccgataatcc agaaaaatcg accaaccctg
     4801 gcatcaagaa tctttggcgc ctttacgacg aaaacggcgc cgctcgactc gatcttattt
     4861 cttgtcaaga tgagaaaata caggaaggac tcgaatatat cgtgcatcat ccaagtgcgg
     4921 actggcgtcg tttgaaaatc acgccagcta gggtagagca tcttcttttc aaagtgatgg
     4981 acagaggaaa tcgaaccgaa actcttccca atataaagca gattcaggca ttcatgaaag
     5041 agaggataca aacctttgat tcgacatatt tgcgccttct caatccgcat atctataagg
     5101 tttctattac ggacaggctg tacgatctca aagtatatct aattaattct tttttaaagc
     5161 ataagctatc gcggaactga attgctttaa ttgtggtttc tacgctctgg cttttttaga
     5221 aatggcgccg caccagaggt gccagttttt ttacagaggg cgtcgagcga atatgctata
     5281 ttatcgctca caataagatc ttcatagatt tctttatgta ggaatccttc tttgaccata
     5341 tgcttcagca tttctaaaag gtgatcatag tagtgctcta tatttagcaa ggctacaggc
     5401 ttttgatgat atccgatata tcgccacgac catacttcga aaagctcctc cattgttcct
     5461 ataccacccg gcatgcagag aaaagcatcg gcctttttct ccattagagc ttttctttca
     5521 tgcatgtcgt ttaccacaag caattcagaa agctcttgct gatcaacaag ctcgtagaga
     5581 cgctgaggaa tgatgcctac aacttttcct ccattcttga tcgccgcctc ggctactatg
     5641 cccatgaggc ctacatctcc accgccatat acaagaacca tccctcgctc tgccaatata
     5701 cgcccgagtt cttctgcggc ctcggagtac gaagggcgcg ccccagttga agatccgcaa
     5761 aaaacacata agttaagagg ctgcaaagcg acgctttcct gtgagatatt catatatttt
     5821 ctctttgatt gcacaaatcc attttgaaca atctagcgtg tatgaataga ggattcaaga
     5881 tggaggtggg gggaattgaa cccccgtccc catgcgtgcg ccgcacgcat ctacaggttt
     5941 atctggtcaa tgcattgtcg ggagccgggg cacagaccag cacgcggtcg gctccttatt
     6001 ccggtttaat gtccctcata ggcaccggac caccagtgag gtgagctccc taatgtcaca
     6061 gacatccatg gcgtcggaag ctgtccgcca tggcgtccgt cgctcactgc ttacgcagcg
     6121 agagcgagag cgccgttttt gttggcactt aaaggttttg ccacgttaag gagagtggct
     6181 actccacctg cagcgcacac cacaaaccgc ataggtcgaa accagtgcac ccccaaggtt
     6241 atgcttcata taagaataag gcaattctgt tatagaatca agcgctctgc agcaatcttg
     6301 gattcgaggt ttagggctgg cttaagagag ttgggagctc tattcctcaa ttttgccggt
     6361 atcggttaat gccttttcta ttataccacc cccaagcact agaccttctt cggagtaaag
     6421 gactgctgat tgaccaggcg caacagctcg ttggggaatt ataaattcta tgcgcgcagt
     6481 tcctgctttg tcgaactctg cgattgcctc tgctggctta tggttctgcc ggatttttac
     6541 tttacaagca atgcgctctc caacagactc tggattctgc agtctaaagg atcgcgcaat
     6601 tagtccagat gaaaaaagcc catcaccaga acctacgata acgcggttcc gttctgcatc
     6661 caggccaagc acatagactg gttccgtacc taaagaaatt ccaatgccac gcctttggcc
     6721 aattgtatag aatggcaatc ctcgatgcct accaatcaca ttgcccttca aatctacaat
     6781 gtcgccaggc aagggttctt tattttcaaa aagaggggca tagcttcctc cgccaacaaa
     6841 atcctggctt tcggattttt cggctgcttc caatccatag atatgcgctg cttggcgtgt
     6901 ctcttttttt gtcatctctc caagcgggaa tatgatgtgc gataggcgtg ctgaatctaa
     6961 accatatagg aaatagctct gatctttatc gatatctcga gctactctta tatatgtgat
     7021 accatttatc gtactctttc ttgcataatg tcctgtggca aacatatcga atgataggcc
     7081 ggattgtctt gctctatcga tcaagaatcc gaacttgaga tcgcgattgc agactatgca
     7141 aggattgggt gttctacctg caaggtattc gctgcgaaag tattctagca catatagttc
     7201 atattcatct gcgaggtcta tgacatagta aggaatgccc aagcttgtac atagacgctg
     7261 gcatgcctcg atgtcttctt gctcgctagg gccaaagcat gaatgtttga gattttcttg
     7321 tattttatat gcgcctttcc atatcttcat ggtgaggccg attacattgt atccctgctc
     7381 ttttagtagg caagcgctaa gagatgagtc cacgcctcct gaaaggccga ctgcgacagt
     7441 aatatcctga ttcataggta gcgtacttta ctaggaatgg tatttttatt ccagcattat
     7501 aatttgtaac tcagattcaa ttgcaggcat tttccaagaa tttgtacttg ctttattggc
     7561 tatatgcaga gtaaagtagc tgcatgcaag acgtttttat aggttttact tccattacat
     7621 ggaaaatgct ggtgatgtat ggcataggcg gaattcttat ctggcttgca attaagaagc
     7681 agtatgaacc aatgcttctc cttcccatag gttttggagc tatactggta aatctgccac
     7741 tttcgattgc atgggaacac gaggggatac caggatttct taggattctt tacgaggcag
     7801 ggattgctaa tgagctattt cctattctca tttttatagc cgttggagcg atgatcgatt
     7861 tcggtcctct ttttagaaat cctctaatga tattctttgg cgcagctgct cagtttggaa
     7921 tattcgcgac aatgatcgtt gcaacacttc ttggcttcga tctaaagact gcagcttcta
     7981 ttggcattat aggggcagct gatgggccga ccgctatcta tgtggcgaat aaattcgcaa
     8041 gggattatct ggcgccgata tcggtagctg catattcata tatgtctctt gtgcctatta
     8101 tccaaccgcc ggtaatacga cttttgacga caaaacagga gcgatccata catatgcctc
     8161 tgcatgaaaa acaatcgccc cagtggctca aaattctctt tccgatcttg gtgacgatga
     8221 cctcgggtat cgttgccccg atctccgtgc ctcttgtagg ttcgcttatg ttcggaaact
     8281 tgatccgcga gtcgggtgtg gtagaaagac tttctcaagt cgcacagaat gaactggctt
     8341 acctggttac actgctcttg ggcatttcta ttggaggctc tatgtcggct gaaaaatttc
     8401 tcaacttcga tactcttctc attttggcca tgggccttgt tgcttttata ttcgatacgg
     8461 ccggtggggt actttttgca aaacttgtga atatgttcct tccaaaagaa aagaaaatca
     8521 atccaatgat aggggcttgt ggtatctcgg cttttccgat gtctgcgcgt gtaatccaaa
     8581 agatggccaa tgatgaagaa agagggaata tcatcctcat gcaggccatc ggagcgaatg
     8641 tatcgggtca gcttggttca atcattgctg gcggactggt gctcgctctt gtgcccttaa
     8701 tagtcgggta ggagttctat aatggcattt attgataatc tgcgaaatgc aggcactgca
     8761 atgcaggcaa ttgctgtctc tatcggaggc cttcttggtg tttttactac attggcattt
     8821 ttcttcttga tgatcttggc ggcagatcgc tttagtaagc acaaatgaga atagaggaga
     8881 ctgcagcccg atttttctaa gaaatcaacc tatttagtgt ctcttttggc aagactcaga
     8941 cagcctttac ccatttagca agctcccggc ccttatcttt tagaagctcg catgttgctt
     9001 catccggagc tcctggccat tcgactggtt caatgcaact ccatttaagg gattcgatct
     9061 ttgcctcaaa ttccttttta gctcctccta cccaaccata gctgccaatt cgaagtgact
     9121 tgcgcttata gaggtgcttt cgctcaaaga tatcgagaat ataggccatt ggtgggaaca
     9181 tcttgtattc ataggtaggc attgcaatca atataccttc gcttttatag gcatcggcaa
     9241 gaacaaacga aacgttttca tcggggactc tatggatcga ataggggact ttttcttctt
     9301 ctatgccttc gatcacatgc ttgagtccga gctcggtatt tccatacatc gagccccaaa
     9361 ttatagcaat ttctttttcg cgaggtccat ccgaataatc ggcatagcgc aaatagcgtt
     9421 cgatgatctt atttggattt gctctccaga taatgccatg gcttggagcg attaccctta
     9481 tgggcaagct ggagagcttt tcaatggctc tctttacaaa aatgccaaag cttgcaacga
     9541 tatttgcata gtatcgaagc gattcagctt cgaaaaattc atgctcatct tctgatattt
     9601 catcatcgaa aattcggtcg ccaagtgctc catacgagcc aaagccatca catgaaaaaa
     9661 gaactccacc ttcttttgcc caggtcatca ttgtttctgg ccagtggaca ttgggagttt
     9721 cgaaaaattg caaggcttgt cccaaaccaa gatctaaaat atcgccttct tttactgctt
     9781 ggacacgatc gtgttcgaaa tagaaatttt ttacaagttc tgcgcccttt gcagtggtca
     9841 gcacaatagc ttttttattg atcgagagaa attctctcag ccaaccagtg tgatcgggct
     9901 cgaggtgatt aagtattata tagtcgattt cttctagagc tacgccaagc gaagctagtt
     9961 gactcttaag ctcatttgga gcgcccaccc aatctctgac aaggtcgatt aaagctattt
    10021 tgtcggcttt tacgagatag cagttcagcg aaactccata aggaataggc cagatacctt
    10081 cgaatagatc gtcgctccgt atatcggcat gaagcgcaaa gatatggtcg ttaattggat
    10141 ggactttcat tgcatatctc cttgtttcga attatataca atatatgggg taactattaa
    10201 ttctaaaatg ctcagaaatt gcccgtgcct gccgatagct ttattccaat agtaacaggc
    10261 ctgccccgac ccaagaagcc atattccgta ctatccgctc cgaatataaa tgtagaaaaa
    10321 attgatagct tgagataatc agtgatttcc cagctcaccg ttggttgcgc aaacccagag
    10381 atatcagaaa aattcataat acccaaggct gatatcgaga aatcgctacc acctatttca
    10441 gagaatgaga tgcttgcagc tccatagtgc tgaccggaga gaaaaggaaa gctttttaag
    10501 tttcccgttg ctatggaata tagaattaaa ttcttgactc cagcttgctg agattcatag
    10561 gaattgaaaa gagcggctct atcagcatca gaatatcctt cgccattgaa atagtattcc
    10621 gcaatagccg taaaattgct atttgggttg ttatatagga aacctattgt agccgaagga
    10681 tataaggtat ttcgaatatc gtctgaagat atttcattat agtatggata actagattgg
    10741 aaagatttat aaaaagtctt cgggctgcca cgcgctatga ctccttcggc aaaaacatct
    10801 agattccaga ttggaccggt tagtgtcaaa gcagcacgtt cggtagtgtc atatctatag
    10861 taggcgctta gacccaattc ataattgcca agaagaaatt ctgcctttgc agcgagagca
    10921 gtattttcga aatccggatt gtctgtatca aaaatagtat agaagctcag tgtgttctgg
    10981 cttggtccat aagggatgaa tacacgaaac tgtaatgggc cttcacgctg cgccgttgga
    11041 tctaaaatat ttatcgattc caggttgatt atatctgctg gactaaagaa gtatccgaca
    11101 ccccatttta ctgtagcctt tccgaaacgc atatatgcag tatctccaag ctggaaatcc
    11161 gaaaacagtt caaaaatgcg gatatctgga acagagacta atgtagtttt gttgttggaa
    11221 tccaagaggc tctttgtctc tgaaaatggc caagcggtct tgaatgaacc atgtaccctg
    11281 aaatcttctt gaggtcgtgc atcgaagtat acaaggctgt ttaaatcagg ttttagcttt
    11341 tgggaatctg gatctacaag atcaaagcct gctgtccaag gatcattcca ttgccaagaa
    11401 gattgtacag ttccgctaat actaccaccg attttaacag tctcggtttt caagaagctc
    11461 tgaagaggat ttgtctgtct atttagagaa tcttgtgatt ctttttggct agaagtttcc
    11521 ctgtcttgct gtaccactat ttcatcttca aatagtgcgt cgatatcatt gctatcctct
    11581 tgcgatgata tgctgaaagg cgcagccatg atgattagac atagaatacc aattgcctct
    11641 aatctctgaa gagaactcat cactacctcc tttattacct cctatattac ctgcaattac
    11701 ctgctaactt gttcaaggaa ggcttttgta aacactctat cgggtaaagg agacacagat
    11761 tgttctgcca tggtgatctg gctcttttct cctttgttga cttcatcgac aatcaatatc
    11821 tgcgaaggaa gaagtcttcc ttcaagctcg acatatttag gatatgcggt agttcgcatt
    11881 agccgtccat tcacagagta atcttctcgt ttgagcagca ttgtcctatc tttgcgaata
    11941 tatagcacta cccgatcata ggctacttca tttgtttttg cttttagatc caatatccaa
    12001 acaggaaact tgcccaccat accatcggtc attttcgtca ccgtataatc ttcggcaaat
    12061 gtgctgagcg taaaatcgga attctttgct ttagtgttct gcaagttttc tttcatcgaa
    12121 gaatgcgaaa acttgcggct tgtaggatca taaaaccaga cattatcatc ttccctgaga
    12181 taaccctgtc ctttgttcac ttccggaagc agaatcaaaa tgacaaattg ctttttcaaa
    12241 tctctgcgga agatccgagc ctgtgtaacg ctgtcttttt ctcctggttt ttgggtgaca
    12301 atagtaaaga cagcagaaaa atctttacct gaaaagtcgc tcaaatcatc gacttgcttg
    12361 ataagtgcct tcggatctgg ctttgactga gcaagcaaag gattcaatcc aacaaagaaa
    12421 agcattgcca agataatgat aaaatagctc tcttttttta ggcctcttgt tttcaggaat
    12481 acattagagt gcatgctaaa aactcccatt tatttgacgg ttctcaaagc ttctgctggt
    12541 ggcaattttg ccgcagcatt tgctggcgca tatactgcaa cgagtgtaag caccgctata
    12601 agaagaatat tgcctattgc gcgaagcggt ggcacataga atgaaaaatg accgcgcttc
    12661 aaaatcaaga atactggaga aagtggatca aagcttataa aagtcaagag gcccatagcg
    12721 attagtgcca agattattcc tgcaatggca cctgccagcg caagcaatag tgcttcatat
    12781 agaaacattg atcggatttc attcctttga acaccgatag cccgcatcgt tccaatttct
    12841 cggattcgct catacattgc cattcggaag gtattagaga tgccaatcat aacgatggcg
    12901 aagagaacga ttaaaaccac tgtgcttcct gtatctagag caactactat ctgctgtgcc
    12961 tgggcaagaa catcatcgat tgtatagagc ctatatttgg tccctgtcca ttgttcggtt
    13021 ttttgctcga gaagcatcgc ctgaaatggg gtcatggaac catcttcctt ttttgtcgaa
    13081 cgatcgaaga gattgagtcc catgcccttc atcgaagtat atagcgcatt agcgaaaggc
    13141 cctgatttct ctagtctttc tagcattatg ccaagagaca tgtattcatt tttccctaga
    13201 ccgatgagac tattgagata ttcaagttgc acgtaggtca tcatagagcc gacaaaactt
    13261 gaatctatag ttattgctgc gacgcgaaat tcgccaacat tgttctgacc tgttatagtc
    13321 tgcaattgtg caaccaatac atctcctggc agaacattga gtttttttgc aatgctttgg
    13381 gatattatta tagcattagg ccaggaaata ttatcccaat tcccgtcaac aagagcaagt
    13441 cgttctttta tcattccaga atgagacaga tcgatgccgg tgagtgtctg ctgcatgctc
    13501 ttgccctcaa aaatcagcgt agctatggtc tggctggttt tctgaaggct aacccatttt
    13561 tgtccagaat cctctaaggc ttgcattatc accgaatcat cgcgaattat cgatatacgc
    13621 ttgcctgacg gagtatattc ggatccacta acgaagacat ggccgcccat caggtacgaa
    13681 aaattatccg atatattttt tacaaatgat ccagcaaatc cgttgataat ggttacgatc
    13741 attattccaa aggctattgc tcctgcaagg agcatagttc tctttctttg ccgcattaga
    13801 tttcgtgcag cgatgcggag caatactaca ttcatgtact tcctcctatt cttcctgcat
    13861 cgctctcact ggctgaattt tcaaagccag cgaaactggg tagaggtggg ctataaagcc
    13921 taccgctatc atcagtacga ttgcaaaaaa tacatttcct gctgttgcaa tggggtttaa
    13981 gtattgccca ccgaatagaa caaggaaaaa cggatttgta gcctcgattc ggagcgattt
    14041 aaggatttcg ataatcaaga ggcccagtcc tgccccaatc gaagccgaga ccagtgcaag
    14101 agccattgct tcagccgcaa acatggtacg cacaaaaccc ttgccggccc caatggcacg
    14161 catagtgcct atttcgcttg ttcgttcgat tacagaaatg acaaaggtat tcatgattat
    14221 tatgatggcg acgatggaga gtataataat tgcaaccgca aagataagac gaacaacatc
    14281 tacagattgc ccataaggac ctgcggcggc ttgccaacta cctgcaacag cctgaattcc
    14341 ttctttttca aaagccttat ttaaagaaga aatgatgcga tttatatttt gaggagaatc
    14401 cgctttacct acaatgaatt gccatgctcc ttcatcaggc tttagaggat ctatttggct
    14461 gggtttggat aattccctag agattgaact tacatcgaaa tgttctgaag tctccgaagt
    14521 tacgattata tcatcgctga aaagctcatc gggatcttct attgaaagca tacttgtctc
    14581 ttcaggcttt agatgtattg gttcattggc tccaacggtc atgccgctca ttacgcgaag
    14641 agattcgata tcgatatagg caagctgctc aggtgccgtg ccttgttctt taagccggaa
    14701 gatgccgaca ataggaagct ccctcaagcg aaaaccgatt gtagaaaagc cctgaacaag
    14761 aagaggatct cctatcgaaa gaggtttctc gaaccaatcc gaaaatttat agaattgact
    14821 ctcattgatc ataatgccag attgccctgg ctttaatttt tctcctgcga caatctctac
    14881 ggtttcaaat aggtcccaat atgtggctgc atcgattcca aaaagaaata aaaatctatc
    14941 ctgaggcttg attttgtttt gggaatcctc ttttgtcatt tgatcaccat tttcgcgaat
    15001 tacctgagaa aaacctgttg cgagcgaaga ggttttcttt aggtggggag tcgattttac
    15061 gatagaaagc accttatcat actcaggaat tatgggagtt tctgccatgc cgctggtgga
    15121 agtgacgccg aacagcgata ccggaccatc acttgaaata ccagagataa aaatatctcc
    15181 tgtatataca tctgaaaatg tcgagcgaac cccttcctcg gttgcattga taaaggctgt
    15241 gccaaacacc aaaaccatgg ctcccattgc taataaaatc ccgatgatga ggcttttgga
    15301 tctatgggcc ttaatattgc ggaaagctat cttaaaaatg acggccattc ttaattctcc
    15361 gccgacgccg taaaggcatt ctcttcaaaa atatcttcgc cactccgcct attctctatt
    15421 accatgccat cgcgcaagcg aataacatga tcggcgatat tgacgatctt tgcatcgtga
    15481 gtagagaaaa tgaaagtcgt ctccaattct cggtttatct tcttcatgag gtctattatt
    15541 tgttctcctg ttgtggaatc cagattagcg gtaggctcat cggctagaac tatagaaggc
    15601 ttagtaacca gagcacgcgc tattgcaacg cgctgacgct gccctccaga tagctcattg
    15661 ggtttatgat tccgccactc tgtaaggcct acttcgccaa tgaggaactc tatccagctt
    15721 ttccactcgc tcttggggac ttgggtttta ccaagaagca gcgggaattc aatattttcg
    15781 tagacattta agacaggaat gagattgaag gattggaaaa taaagcccaa aatttcatgt
    15841 cgaaatcgag taagttcttt atctgaaaga cctttagtgt tctttccgtc aataataagt
    15901 tcgcccgatg tcggtacgtc gatgaggcct attatgttga gtatggtaga ctttcccgag
    15961 cccgaagggc cagcgataga gacaaaatcg cctcgttcga tagaaaaaga gattcccctg
    16021 agtgcgggga caactacatt ccccaaataa taatcctttt ttacatcgat cacctgaatt
    16081 attggcatgg tttatccacc ttgggaaaga ttagtgttac taggagaaaa tatcgatttc
    16141 tgtaagatat gacaatatga gtctaaaatt aaaaatgatt tcgacgccga tttaataaac
    16201 tagagtactc gtaaaatata tccctatatg catgacagat actcgcccaa tgcaatatag
    16261 agaattttgt tctagttcaa aagtccttcc taggcgcctt aaaggccagc ctcatgcgct
    16321 tctatttctt ctttgaacgt gctagcaagg cgcttcactc cttcctcgat ctgttggtag
    16381 ctcgaatagg agaagttgat gcgcatggaa tttacttcag gatcatcata ataaaaagaa
    16441 cttcccacca cataggcgac cttcttttcg attgccttgt agaacatctt atctgtatca
    16501 atgtagctag gcagagacag ccacaagaat aagccgcctt ctggttttgt ccatgtgaga
    16561 tctggatgtt tgggcatata ggtatctagc ttttgtagca tgaaattgcg cttatcgcgg
    16621 taaatggcac aggtattctt gaggacttct tcgagtttgc cggttgccat gaactgagcg
    16681 acaatcttct gggtgagcgg gcttgtgcag agatccaccg cttgctttgc aacaaccaat
    16741 tgctgaatga tatccttgcg tgcaagaatc cagcctattc gtgttccagg cgcaagtatc
    16801 ttagagaatg ttttaaggcc aatcacaatg ccaaggttct gcatttccag ttctagctgg
    16861 tacaaggaag gtatgggttc acccatgaat ctgagttctc gataaggagc atcttcaacg
    16921 atgaaaagcc cttcgcgata tgcaaattct ataagggctt tgcgcttttc aaggctccag
    16981 catactcctg ttggattttg aaaatccgga atcacataga tatattttat tcgcttattc
    17041 tcactcttgg cgcgagcgta ttttctctct agctcatcca tgtctaagcc atcgttagca
    17101 tgagagaagg gaatgccaag taccttgcct tgataggatt ggattgcttg aattgctcca
    17161 agataggtag gacgctctgc aatcacgtat tctcctgggt cgaggaagag ttttggaacc
    17221 atatcgagag cctgctggct tgctgaagtt atgagaatat tatctggatc gagatcaatg
    17281 ccgtgagatg actcgtattc gatgattcga gcctttagat cattatcgcc ttccgtcgtg
    17341 ccatattgga ggattttgtc gccatatttt ctaaaaacct gatcgctcgc tactctgagt
    17401 tcttcgatgg gaaaggtctc tggagcagga agccctcctg caaatgagat gatatccggt
    17461 tgattagtaa gcttaagtaa ttctctaatg acggatttct tcatactatg ggcatttgcg
    17521 gacaaataca ctgaaaggtc ctgcattgat aagtcctcct tgatttgtgg ttcttgtttt
    17581 tatcctactc cagtttttat gattcgtaaa tatatgtttt caaaattcta tactagcgtt
    17641 tcattatttg aaataataag catggtactt gtcgtatgat ttgtccttgc tatactttgg
    17701 aaatcaagag accaataaga ggaatgtcct aatgccgcta aattgtggta ttgtgggttt
    17761 gcccaatgta gggaaatcca ctattttctc cgctcttaca agggcaaaag tggatgccgc
    17821 aaattatcct ttttgcacaa tagatcccaa tgtaggcatt gttccactgc cagacaagcg
    17881 cttggataag ctattcgaga tattcaaacc aaaaagaaaa gtacctgcag ttgttgagtt
    17941 tgtagatatt gcaggtcttg ttcgaggagc ttcgaaggga gagggtcttg gtaatcaatt
    18001 cctttctcat attcgcgaag ttggcatgat agcccatgtt gtgcgatgct tcgatgatcc
    18061 caatgtggtt catgtggcag gcaagataaa tccagccgac gacatcgagg ttattcatac
    18121 tgagcttgca ctggccgatt tagaaagcat cgacaggagg ctggataagc taaatcgcca
    18181 ggtcaaggtt caagacccga atcttaaaaa agatatcgaa aaggaaattg ctgtattgca
    18241 gaagctgcgt cctgctttgg aagaggggaa acctgcctct acaatcaatt tggagtccga
    18301 agaactagat tacgcacggc gcagctttct attgactctc aagaaagaat tattcgtctg
    18361 caatgtggat gaatctggaa tcgacaataa taaatatgtc gaaatagtaa aatcaattgc
    18421 tgaaaaacag ggctcagaag cggtggttat ctgcggaaaa tacgaagccg agcttgcaga
    18481 tatagaggat tctgaggaac gggctgagtt tcttcatagt atagggctag aggaaccagg
    18541 acttgatata ctcgctcatg ctgcatacag attattgggt ctccgcactt tttttaccac
    18601 aggagaagac gaatgcaggg cttggacaat taaggtggga gataaggcgc caaccgcagc
    18661 aggcgttatt catagtgatt tcgaaaaggg atttattaaa gcagaagtct atcattacga
    18721 tgatctcatg aattatggtt cggagaatgc tgtacgagcg gctggcaaaa tgcgacagga
    18781 agggcgcgac tacgtggttc aagacggaga cataattttt ttcaagttca atgtctagca
    18841 gagcataaat agctatacat ctgtcaagca tacttgcctt tatgcctatg accacagtat
    18901 gattttggaa caatggataa gctgatcatc aagggtgcac gagaacacaa tctgaaaaac
    18961 atcgatctcg aacttccgag aaataggctc attatcattt caggcttgtc gggttcgggg
    19021 aagagctctc ttgctttcga tactattttt gcagaaggcc agaggcgcta tgtagaatct
    19081 ctatctgcat atgcaaggca gtttctaggg cggatggata aaccagatgt cgattatatc
    19141 gaagggcttt ctccagctat ttcaatcgaa caaaagacaa caacgcgcaa tcctagatcg
    19201 actgttggca cagtaaccga ggtttatgat tatctgcgac ttctgtttgc gagaataggt
    19261 gttcctcatt gtccttcgtg tggtagagaa attcgcgagc aaagtgtcga ccagattctc
    19321 gatgttattc tttcgtggcc aatggacacc aagatacaag tgttagcacc tgtaattcga
    19381 gctcagaaag gtgaacacaa gaaaatactg gaggatgctc tcaaacaggg ctttctgaga
    19441 gcgcggattg acgggcaatt catcgaactc gaatctcctc cagctctcga aaaacagaga
    19501 aagcatacaa tcgagcttgt ggtggatcgc atcaggctat ccgaagagag ccgccgccgt
    19561 gttgccgagg cagtagagac ttcgttaggc atagcaaatg gaattgtaac catcatgcgc
    19621 gagatagatg gccagcaaaa agaagagttc tttagccaaa aaggtgcctg tcctatttgc
    19681 ggaatttctt tgccagagat ggagccgaga cttttttctt tcaatgctcc tcaaggtgca
    19741 tgtcctgctt gcagcggttt aggcatgaac atggagttcg accctggttt gattattcca
    19801 gataagagct tgtcattcaa cgaaggtggt tgcgcaccct ataatcctaa ttcagcatgg
    19861 aatcgctcaa agttcgaggc acttgcagat cattatcact ttagcctgga tactcctttc
    19921 gataagctac cttctaaggt gatagaagcc atactttatg gttctgatga caatatccat
    19981 gttcgctaca acaatagaga taagactggc tatttcgagt atgagtctag gtttcctgga
    20041 atcttggcag acctacgccg ccgctatatg gaatcgacaa gcaacgatat aaaggattgg
    20101 ctagagtctt tcatgatcga aaaagaatgt gaagtatgtc aaggaaaaag attgaggccg
    20161 gaagctcttg ctgtaaccat tggtggcaag aatatctatg agatttcatg catgcctgta
    20221 gaggaatcgg tccaattctt taagtctctt aaactcacca atactgagac tcaaatttca
    20281 aagcttattc tcaaagagat tattgcacgg cttggctttt tacagaatgt ggggctagag
    20341 taccttacgc tcgagcgcca agcatcgact ctctctggtg gcgaagcaca gcggatcagg
    20401 cttgcgacac agataggtag ctctctcgtt ggtgtgctct atatcttaga tgagccatcc
    20461 ataggtcttc atcagagaga caatcagcgg ctaatcgata cactcacata tcttagagac
    20521 cttgggaata cgctcatagt cgtagaacac gatgagcaaa tgctccgaac agccgactat
    20581 atcgtggatc tcggaccagg agcaggcgag catggaggct atgtagtagc tcaaggcagc
    20641 gtgcaagatg ttgctgccaa tccccagagc atcacagggc aatacctcgc aggatctctc
    20701 aaaatcgaag ttcctgcggt gcgacgaaaa gggaatggcg cttgtatcgc tcttaaaggc
    20761 gcaagcgagc acaatctcaa gaatatagac gtaaggattc ctcttggaag atttgtatgc
    20821 atcacaggtg tttcaggctc cgggaagagt actctcttgt ctgatgtatt atatcctgcc
    20881 atttctaacc gagtgatgcg ctcctcaatc cgcgaaggat cgtataagag ccttgaagga
    20941 atagaatata tcgacaaaat catcaacata gatcagagcc ctataggaag gacaccgcga
    21001 tctaatccag ccacttatgt tggggtattt actccaatca gggaactatt cgcaagcttg
    21061 cccgaatcga aagcaagggg ctggaagccg ggacgattta gtttcaatgt caaaggtggc
    21121 cgctgtgagc attgccaggg cgacggtact atcaagatcg agatgaattt tttgccagat
    21181 gtatatataa cctgcgaggt gtgccatgga aagcgattta actccgatac cctagatgtg
    21241 agatttaagg gcaaaaacat agccgatgtg ctcaatatga cggttgaaga agccagcgaa
    21301 ttttttgcct atatcccttc tattgcccat aaacttcaga cgatgctcga tgtgggtctt
    21361 ggctatattc gtctaggaca atctgcgctg acgctttccg ggggagaagc acaaagggta
    21421 aaactctctc tcgaacttgc gcggcgggct acggggcgca cactctattt tctcgatgaa
    21481 cctacaacag ggctgcattt tgcggatgtg cgccaactgc tcgaggtaat tcgtaggctt
    21541 gtagacagtg ggaataccgt agtgatgatt gaacataatc tcgatgtcat aaaacaagcc
    21601 gattggataa tcgacctagg gccagaagga ggtgaaagag gcggtatggt aatagcggag
    21661 gggactcctg aagaaattgc cctttgtaga gtctcttata caggccaata cttgcggcga
    21721 ttactaaaca tctgaattat ggatctggta ttcttgtcaa cactgcattc ttccatatac
    21781 catacgaatc gatgcagcgc aaaattggcc ttcttttttt gattcaatta ttgcttgtac
    21841 ctcttgctgc ccaagctcag attcagattg atgaaggcac ttctaagcct gaagatgttg
    21901 gatccaaatc tgacaaccaa ataactctga ctatcgagca ggctgttagc gctgaatatt
    21961 ttagccttga aaatggaaca aaagagcttc gtcttactgg acctcttgtt atccaattga
    22021 tcgattccag cggagtgaca catacaataa aggctcaata tgttgtctat aatcgtgata
    22081 cgaaagaagt aaaagctata ggctctgtag aatatactcg ccagactgaa tataaaacag
    22141 atatctacag aggtgaatcc atagctgtag atctcgatga taattctggt gtttttatag
    22201 atggctcttt caatatggag tcaacagctt ctagctcccg caaactgata gtccattttg
    22261 agtctttgat ttccagatct gaagaagtga tttcattggc caatggatct ctaaccgcct
    22321 gcgatgaaat aaagcctcat tatattctac gagcaaaaaa gatctggcta ttcgaatcag
    22381 gagattgggc agcatcgaat gcgattctat atgtaggtgc aatacctgtc atgtggcttc
    22441 ctttgttcta ttatccttct aaggcatcga aattccatcc ggtagttggt tttcgatctc
    22501 gtgcaggagg ttttttgcag acaaccacct atcttgcagg agcacagagt tcggaagcac
    22561 agaagtcttc tatgctctca accaccgaag gagcattagg ttcatttgga acttatattt
    22621 caagaactca gccctctagc gaaagcaaga accagacaaa tatagcgatc ttgggggatg
    22681 tctattcatc cttgggggtt tttgctgggc taagaggtga attagcaagt acctattcat
    22741 acgatctttc ctggcttgta ggagccgctt tttcgcgttc tgtttttcta gaatcgacag
    22801 gctattattc tccttatgat agtgctggtg attatgcttc ggtatggaat aaatggaagc
    22861 ttgcatcgat agaactcccc atgcgcattc tattgaactt cgaagcctct tcaaaaaaga
    22921 ataaaacagg actaaagtgg cagatcagtt tgccgttcta ttccgatcca ttcgttgaac
    22981 aggattttat gtatcgaaat gaatcttatg attttttttc tattctaggg aattcttcgc
    23041 tcaagactat agatgaacgc agttctttct cgcaaaaagc aagcctttcg tggtcatgga
    23101 atccttctat tgagaaaagt aattttactt ttaggctttc caacctttca tcatccttta
    23161 attggaagag caaatatgct tctacaactg gtatgaattc tcaagagctg cacctcaatg
    23221 cggtagatcc tcaaagacat ttcttctatc ccgacagtgc gcgcattatt gataatgctt
    23281 tcactatgtc cggtaacatt ctgcagagca agaatatgtc gttaaaatgg actagcacga
    23341 attcaggctt catagaagat cgattttata gttctcaatg gcagaaacct caagatatcg
    23401 acttcggaag ctggtattgg ctatatggtg ggcgcagcaa cgctgacttg acctcttctt
    23461 attcgataga tgatgctggg ttgacctttc aggtttcgac aggcgttcta gggcagctgc
    23521 aatatcgacc ttatctctac gatgaacgta caaatcctac aactgtgcac cctttcctaa
    23581 ttgcagacta cggctacaat acagcgtatt ggagcactgg aaccaagtta ttatgggagc
    23641 catttaagaa atccaatatc ttttctgcct ctaaattctc ttattctatt caggggaaag
    23701 tagctagatt ggcctacgaa gggctagatg gcagtggaat agatgcctac cctatttata
    23761 aactttattg gttatcatgg gacccaacta tgatatctga tcattctatg cttacagagc
    23821 tgtctgcaaa gcttgggtcc acaacagagc gcttgagctt taaagttgcc ttgccaccac
    23881 ttctcgagag ttatacactt tcaggttcga cagccctagg cctagcaacg atgggggcta
    23941 gctacgttat atctcgtaaa gattccctag aggacctcaa gtcgacttca ttgaccggaa
    24001 accttactct aaagccaaca aaaaaaattg ggatttcggc aaatgctgct tgggatttcg
    24061 atactcaggc ccccttaagt ctcagcaccg agctatcggc ttggtcgttc aatgcacgct
    24121 ttgtggctca aaaagcaagc ggctatattt tcaagggtag ttcatggatt caggatggga
    24181 ctcagtattt cagaccttcg acattatccc tatcttggaa acccacaatg cgctcagatg
    24241 tgccattatt ctccaaggat acgattggct ggtctgtcga ggcaaatgga ttgcttactc
    24301 tcaatcagaa tctcattcaa tctacaaatt ctacatttgg agcaaatctt ggcttctcta
    24361 tcaagaattc tacaggatta tcttttaatt ttacgctttc ctctgtcaat tcctccttct
    24421 ggcgatatta tgcatccctg cttccaaaat cagatgactt tgaccctgag cactattcta
    24481 aaaacttttt cttggacctt tgggactcta tgtctatttg ggatatgaat aagctaaaga
    24541 atacgctttt taagttacag agtctaaagg tgggcctttc aatggatgcc catgattggg
    24601 tattgtccgc aaatattgaa gctggaccag agcttgtcac agcatcgtca actcaaccgc
    24661 tgccccacta tgagatggaa gttgccttca acctggcagt tacatggaaa gatatctctg
    24721 caataaaagg ttcgataaat tattctgaag gtacatttaa gcgatgagca tgtgagtctg
    24781 ccttaaaaat ttaacaggcg gtgaaagccc taaaacccgc caataaacag cgaacagtca
    24841 atagacaata gtgcagatga gaccaagagg ctggaaagag cttcttggta ttagtatatt
    24901 attaaagaaa gactattatt tggataggag tgcgattgag tccttcacat attgtgctcg
    24961 aagataccca agtgtttgcc gagctttgtg gcgcaaacga ttacaatcta tctttgattg
    25021 gaagcctttt ggggactcat gtgcttacaa gaggtaatga gctttttgtc gaaagcgatg
    25081 atccggcagt actaagcctt ttctcacgcc ttatcagtgc aattggaact tcgatagaag
    25141 aaggcgtacc tgctactcca gagcttataa tagctctgca tgccgagctt acgcccgatg
    25201 gaagtcaatc tttagatgct tcgatagaga atcccgcgga ggaaaaagag agcgatattt
    25261 caaggttcct cgataattgc atacagatac ccaatggttt tggtaaggtc tttccgcggt
    25321 ctgcagggca gagcctttat cttcaaggca ttgcatcaca tgaccttact ttttctatag
    25381 ggccagcagg aactggcaaa acctatctcg ccatggccta tgcgctgagg gagctcttga
    25441 cgcgatctaa gcggaagcta attttgacac gacctgtagt ggaagcggga gaaagtcttg
    25501 gttttttgcc aggtgacctt gagcagaaga taaatcctta ccttcgtcct ctctatgatg
    25561 ctatggaagc cctggttcca tacgagacta ttaggcggct cgaagagagt agggcggtag
    25621 aagtggcacc tctcgcctat atgcgtggta gaagtcttgc caactgtata gttattctag
    25681 acgaaggcca gaacactaca aaagagcaaa tgaagatgtt tcttacccgg cttggagagc
    25741 acacgaaagc gatcgttaca ggggacatca cccagataga tctccccaag aaaacagagt
    25801 cgggtcttat tcatgccata accctccttg cacatataga tgaagtattt ataagtaggc
    25861 tcgatgcccg cgatgtggtt cggagttctc tagccaggaa aattattgaa gcttatgaaa
    25921 aagaatagtc aagaatcctg gttctttgag ctgaaaaaat ctcttcgctc ggtacaacca
    25981 ccgctttggc tgttctttgc tgcaattgtg atagcggcaa ttggtctgat ccttgttggg
    26041 ccatcatcga tttcaagccg tagctcactt aaagcgataa aagtaggtga gatcgcagag
    26101 actgatattt atgctggcaa agacactatc tatatcgata aagaggcgac acagagaaag
    26161 attcaggctg aagagagatt agtacttgca gtctttgtga tggatgacaa gatcaccgca
    26221 tctattcgcg aaaaggctga agaattctct tcttattatt tacaacttct aggcagtaaa
    26281 tctggaagca tcaatgcctt atcgctaatg cttagcagcc gtttccctga tctcctgcaa
    26341 tccgatcagt atgagcatct tgtccgcgct gagctgaatg cacagacctt cgtatatgtc
    26401 agcgatattg tcgagagtct cttgagcaaa ggcattgtct ccgtgccgga aggtgtcttc
    26461 gatcgattca atccggatta ttatgaacta gtaagaatat tgaatggaag ggaagagagc
    26521 gaaaagatag ctgtcagcag catgattacg cttgtcaatc ttggcaatcg tctggaagat
    26581 gaaatggatg cccggcatat ccctgaatct ctaaagcaat atgtgagatt tttagggaaa
    26641 gcgctcttga gggaaaacgc tttttttgat caaaatcttt cggagaagag gcttgctgcg
    26701 gcacgagctc gtgtagaacc agtaacacgc tttgtgagca ggaatgaaat tcttgtgcgc
    26761 aaaggggagc taattacacc agaagtttat cagcagataa agactattcg ttcttcgatc
    26821 cttgtttcag atatcggcgt acttcttaca ggattagggc ttcttttgat agcttcttta
    26881 ttaggccttc ttctttcgag gatgcccgac ctgtccgatt tccctatgga tcgctcctca
    26941 ctgatattct tgctaatttc gtttctcatt cttttttatt ggattcttgt tattcagaat
    27001 ttattgagtg ggaagagctt ctcacatgga gctctttttg tgccagcatc cttatttgct
    27061 ggccttgtat cgctcctcta tggagcacga acaggtattt tctttagttt ggtttcattc
    27121 tttcttacag gcgccgctac aaatttaaat gctcaattta tgattggtat tctcctagcc
    27181 ggaatttctg caacgctaat tatgcgcaca gcgcacactc gcattatgct tgctagagcg
    27241 gctgtcctgc aggcgcttgt ccaagcgatg ctagcaatga tactcctctt gaatcaaaaa
    27301 ccaagtgcag ccgaagtctt acaggctatg ggcttaggag cgctcaacgg atttgcaggc
    27361 ggctccttta tcctcttgct tttgcctctc tttgagcgcc tgcttgatcg atcaacgcaa
    27421 ttcagactca tggagttagc agatacaaat gctcctattt taaaggagat gctaatgaag
    27481 gctgccggca cctatgctca ctcgatgaat gtagcaaatc ttgcggaagc tgctgctaag
    27541 gacataggcg caaatccgct cttggctcgt attggagctt actatcacga tatcggaaaa
    27601 attgaccacc ccgaatattt tgctgagaat caacaaggca taaatctgca tgacgatatc
    27661 aatccaacac tctctgcaag cataataaga cgtcatgtca aggatggcat agaaagagct
    27721 cgagaaatag gtctaccaaa ggaagtgatt gaaataatag gccaacatca tggcaactct
    27781 gtaatggaag caattctggt aaaagcaaaa gacgctgcag cacaagttga tgtttcgtct
    27841 tatagctatc aaggcgagcg gccaagaagc aaagaagcgg ggatagtaat gcttgccgat
    27901 agcatcgagg cagcctctcg ctctctaaag aatcctaatg catcaaagct tgaggatttt
    27961 gttcatcaat tgattcttaa aaagctcacc aatggccagc tctctgatag cgagctcaca
    28021 tttaatgata ttgaggtcgc agaaaaggcc tttttgcgaa ttcttcaatc gcagatgcat
    28081 acacgcctgg aatatccgga gcagaggaaa caatgaacac aaacgaagta ttttattcga
    28141 tgattggaat tgatgagccc tcttgggcac agagatctgc cgactatgca ctttctgtct
    28201 tggacgcact tgggaaaaaa gactggcaga tttcattgac attctgcgat gataatacaa
    28261 tacaaaccct taacagagac tatcgacatg tcgactctcc taccgatgta ctctctttcg
    28321 ttcttggtga gtatgagcca atagaaggca gtgagagtat ctatctcgca ggcgatattg
    28381 ttataagcat tcctgcgatc gagcgcaatg ccgaggaata tggagttgga gaagatgaag
    28441 agttgcgccg tctaattatt catggcattc tacacttaac tggtatgagc cacgaagatg
    28501 attcgccgga tcagccaatg ctgctgcttc aggaacaact ccttaagaca ttgggaggaa
    28561 atgttattct atgaatattc tttcacaact ctttggcgta cacgggaaaa ccgttgaatc
    28621 aaaggatgaa gtcgtcgaag agcaaagaga aatgatcgaa ggcgtggagc atcttcccga
    28681 gaccattgtt agagatgtga tggttccacg taccgataca gtgtgcgtcg aagatatcag
    28741 tcccatcgat gaaattctcg agactttagt agattctggg cattctcgaa ttccagtata
    28801 ttcaggtacc attgataaca tagtaggtat tctatatgcg aaagatgttc tagctgccct
    28861 tgtgaaaaaa aagcctctgg aagttaagag tctcatgcgc cttccctatt ttgtaccaga
    28921 gacaaagcga atagatactc ttctgaaaga attcaaacgc cgtcatgtac acatagctat
    28981 cgcagtggat gaatatggag ggactgcagg cataataagc cttgaagaca taatcgaaga
    29041 gatagtagga gaaattcagg atgagttcga cgaagaggca gatcaggttg tgccaatcgg
    29101 aaagaatcgc tggtcctgcg atgcccgcat tcatctagaa gacctcaatg atgcaatagg
    29161 cgccgagttg ccttatgagg aatatgatag tctggcgggc tatgtttttg atctttttga
    29221 gcggatacct tcaaagggag agcaggttac agtcggtgat ctgcaattta cagttgaaga
    29281 gatggaaggc cacaggttga caaaaatctg catagaaaag cttgacatga gccaagaaga
    29341 ttctcctttt acacctatgc atgtttcata aaatgaaaca tagccctaaa tcccaatttt
    29401 gcttgcggaa atcgataacg gggtatagaa tcataagagt gatctttacc ccacagatca
    29461 attaacaaca atccctaaaa ggagggagaa atgaattcct acataaaaga agtgctggat
    29521 gggcttcgcg ctcgatatcc gtgggagagc gaatttttac aagctactga ggaagtcctc
    29581 gaatcgatta ctcccctcat cgaggcagag ccgaaatatc gccagcaaaa gattctagag
    29641 cggatcgtcg aacctgagcg ggtagtgata ttccgtgtgc catgggtaga tgacaaaggc
    29701 gaatatcatg tcaaccgagg atatcgtgtt cagttcaatt ccgctattgg gccttacaaa
    29761 ggtggcatta gattccatcc taaagtcact ctctccaccc tcaagttcct tggatttgag
    29821 caggtattca agaattcgct tacaggtctt ccgatggggg gaggcaaggg tggttcggat
    29881 tttgatccga atgggaagag cgacaacgaa atcatgcgat tctgccagag cttcattacc
    29941 gaactattcc gctatatagg cgcagataca gatgtgccgg caggcgacat tggagtaggc
    30001 ggccgtgagg taggctatat gtttggccaa tacaagagaa tcgcgaatga gttcacgggt
    30061 gtctttactg gcaagggact gacttttgga ggttcgcttg ttagaccaga agccaccggc
    30121 ttcggcgcgg tgtattttgc tgaagaaatg ctcaagacca tgggcgacac aatagaagga
    30181 aagacagtgg ccatatctgg ttttggcaat gtagcatggg gcgcatgcat gaaagctagc
    30241 cagcgcggtg ctaaggtgct taccatttcc gggccggatg gctatatcta tgatccggac
    30301 ggggtcggca ctaaggaaaa gtgggactac ctcggcgaaa tgctcataat agaccgcaac
    30361 aaggtcgaga actatgtcag gaaattccct aacgccgtgt tccacaaggg caagaaacca
    30421 tgggagcaaa aggtggattt agccatgcct tgtgctatcc agaatgagat gaatggcgaa
    30481 gatgcaagaa tgctagttga aaatggcgtc aaagttgttg tcgaagtttc gaatatgggc
    30541 tgcatgcctg aggcttggaa gtactttata gagaagaaga ttccttttgg ccctggtaaa
    30601 gcagcgaatg caggcggcgt ggcaacaagc gggcttgaaa tgagccagaa ttcgatgcgt
    30661 ctatcctgga gtgcagagga agtggacaga cgtttgcatg aaataatggc gaacatccac
    30721 aagagctgtg ttgagacagc ggcaaagtat ggttttgaag gcaattacgt cgttggtgca
    30781 aacattgctg gattcaagaa agttgcagac gctatgattg cacaaggact tgtgtagttg
    30841 tagagggttt tgcatagaac caatgggaac tatggtcgcg aagccgggga gagtcagcgg
    30901 tagcatacaa gccccggcat cgaagagtgc cctgcagcgc gcgatcgctt gtgcaattct
    30961 agcgcgggga ataagccaca tagaggcgag ccccatttgc agcgatgcgc gcgctgcaat
    31021 ttttatcgca aaagagctgg gggcaagggt tcaggaagaa aaaggaatcc ttgaaattga
    31081 aggatcggct ctatttcata aagatactga agtcgaatcg actattttat cgaagaaagt
    31141 atcctatcca agcttgcaag atcagagatt tcaatcgtac tcaccactaa tccttccatg
    31201 tggagagtca gggctctgca tgagaatgtt ctctcccatt gttgctttat tggacaaaga
    31261 ggtagagctt cagggcgaag gttctctttc gagtcggccg atgaatatgg tcgaggagtc
    31321 tctaaagagc cttggcattt tttgcgtatc gagaggtgga gttcaaccta ttcgtattcg
    31381 aggtcccctt cggcccttgg aaggaagggt aaatgcctta ggtagctctc aattcattac
    31441 gggccttctt attgctttgc ctcttttaga tggtgattca gagctgattg tagataatct
    31501 tgtgagctcg ggatacctcg atctcactat tgaaatgtgt gcagaatttg gggtcgaaat
    31561 aaaacgagaa gcaaatggtt ctaggttttt agtaccagga tatcaggtct acaaggcagc
    31621 cgatcttgct atcgaaggcg attggagtgc cggtgctttc ctggccgtaa gcgctgcaat
    31681 ttcaggaagg gaagatggcc taagaatcca gggtttacgt caagaatcaa cacaacctga
    31741 tcgtgctatt gtggatgttc taaccaaagc tggagcgcaa gtgcaattcg aaggctctga
    31801 ctgcattgta agagctgcca accttgttcc ctttgatttc gatgcaaccg attgcccaga
    31861 cctttttcct cctttagttg ctatggcgtc tgccattcat ggcagatctt gcattagagg
    31921 aacaaggagg cttttatcca aggaaagcga tcgctcggca gcattgtgca aaacctttcg
    31981 gaagctaggt gcagaggtaa cattgcaaga cgatgcgatg tacatagaag gtggctctat
    32041 tcatgctgct actattgaat catttggaga tcatcgtatt gccatggcag cctccatcgc
    32101 agcgctcgtg tcaagtggcg aagtatacat cgaaggagct gagtgtgtat ctaagtcctg
    32161 gccaggcttt tttgaagatc tttcttctat attggtttct tgattatagc ttgaaaaaat
    32221 cttaaaagtc caataaatat tggttcgata aatatctgag actcggcagt aaaagattca
    32281 agccttctat tctattgtcc tatattagcg cgcagtttat caaggaactt cttcatttca
    32341 tcataatctc gttgtcgaat tatatgagtc atataagcta atttagagtt tatgagctcc
    32401 agctggcgta tggtataggg gttgaacata atctctgtaa gaaggcgatc gtcttctgat
    32461 aacaagcctt ttgcaacggc taagtgtcgt ttgaagttcg ttcctggtgc atcctgcttt
    32521 ttcatgcagg ctgcgaatac cattgttgaa gcaaaaggtg tagccaacga ataggcgaca
    32581 gtcttgtcat gttcttcaaa actatactcg aagatgcaaa tctttaagct tgaatagaat
    32641 cgacgaaaga aatcggcgcc ttctttgctc gattctgaaa tgatgattgc gctctcttcg
    32701 tgaaggtccc taatatttgc tagggttggg ccaaacattg gatggctaga gacaaaagga
    32761 tgtccacagt tttcgtaata ttccttaaag cctgttttta cgcttgctat atcgctgatg
    32821 atacattgag aaggcaaata tggaagaacc gtatcaaagg cctcaagtgt caaaccgaga
    32881 ggtacgcagt ttataaaaag ctcaggcttg aaatcggcaa attcctcgat ggatagtgca
    32941 cggtgcacct tgatgaaata tttcatcttc aagagatcac tgtcatgaac catgacttca
    33001 ttgttccagc atagctcctc cgtgagccat gcacccattc ggccagcgcc aagtatagca
    33061 attctcatga ttctaccctc atttcatgat agcatcccag aattttaaaa ccttcggcta
    33121 tctttggaac cttttcaagt gctcgttgca cgggagcaga ggataatgaa ccttcgaagt
    33181 cgatgaatat agcatacttt tctggaacat cgggcaccga ttctattctt gtaaggttta
    33241 tattttcttc agcgaatacc tctagcacag aaaacagggc tccagctttg tttccagcgg
    33301 taaaaacagc agaacacttg tctccctcga ccccgttatc ctctgtcgcg ataataaaaa
    33361 agcgcgttct attatgggga gaatcttgaa tatcctcttt gataatctcc aggccataga
    33421 gatccgatgc aaacttgctt gccacaacag atacgccttt aggcctgttt tccgcaaggg
    33481 cctttgctgc acctgccgta tcgtaaaagg gctccggatc caaatgattc cttaacagaa
    33541 agttacgaca ttgtgtaagg gcctgtgtat gagatcgggc tatcctaagc tcgcgatggt
    33601 cagctccagg aattgtgagc agacagtggc gaatgggcaa gtcgatagct gctactattt
    33661 ttaggtgtgt atatatgaga atagagttta caggaccaac tatgcctcca agggtatttt
    33721 caacaggaac gatgccatag tcgtaaatgc ctttctcgac accatcgaat acatctgaga
    33781 attcgagaca tggtatagtt gctgcctctg gcatgagcaa tcgcagggca acttccgaat
    33841 aagctccatg ttcgccttga aatgctacgg tcttaagccc tgcgtcctga agacgtcggc
    33901 attccgctat tatctgttca tagaggtgga ctgaaaaatg agggtctagc agacattgac
    33961 tagagcgtct cactttttct tggacaatct gctcgcgcac aggatcaaag gctgtagcct
    34021 taaagcgcct tgctaagagg gatttttcca ttcgctcgtt taagagcgac agtattttcg
    34081 catctatgcg atcgatgtcg cttcgaatat catcgagatt catgatttcc tccatggaat
    34141 attgcatgca aacggaattt ctgctcgcgc tagaagagct aggtcggcca gggcacaagc
    34201 tgttgctgcc tctaggacca ctgcgcttcg aagagcaatg caggcatcat gccttccttg
    34261 aatttcaagg tcaaccattt catcgcagga aaagtcgaga gtgtgctgtg cagatgcgat
    34321 tgaagatgtg ggcttgactg caattcgaag cacgatatca ttgccatttg tgataccacc
    34381 attgatgcca cctgctccat tgcgagctgt tcttccttgt aggtcaataa atggatcatt
    34441 gtgctgcgag ccgcgcatag caactgcgga aaaaccatcg ccaaattcca caccgcgtac
    34501 tccgggtact gcgaaaatat aatggctaat gacactttcg gcagcatcga agaaaggttc
    34561 tccccatccg gcaggtactc cagatattct tatttcaaca agagctccta tcgaatcgcc
    34621 agattctttt gcccgaagaa ccgctttctc tatatctcta gacccaccag cttcgagcaa
    34681 ctttgtttga aaaacaatat cgaaaaaggt gttgaggacc ttttttgcga caacggcagc
    34741 agcgacaatt ccaatggata tacgccccga aaaatgccca gatccccttg gatcctgcca
    34801 tccctgatat ttcatcattg cactgaaatc tgcgtgccct ggccgcggaa tattggtaaa
    34861 ttgctcatag tctgaggatc ggacattatc gtttcgaaat gcaatatgaa tgggcgcacc
    34921 acttgtatga ccgtcgaaca ctcccgatag gataattggc ttatccgact ctattctggc
    34981 cgtagttcct tctgctcccg cttttcttcg attcagatct tctgtaaaat cctcttcggt
    35041 gagttgaata ccagctgggc agccatcgat tacaactcca acagctttac cgtgagactc
    35101 tccatagaga gaaacacgaa aaatccttcc tactgtgttc atggttcacc ttttccctta
    35161 ggagacctaa gcatttagaa attccacaac atattttatt gcaataggct cgatgcggac
    35221 attgccaatc ttgcttaaaa gtgcaaagtc tatgctttct cctcgccttt ttttgtcagc
    35281 ccgtatgaga tcgcatattg ttgacggact gatattgtaa cctgcaatcg ccgaagcctc
    35341 tttgatattc gttggcagac caaggttacg cagcaataaa tctatgcgtg ccgcgatatc
    35401 tggatctagc acaccgagag atactgcgag agcgtttgcc atacctattc ccgttgccac
    35461 ggcaaatcca tgcgggattc ccgtaataag ctcgattgca tgaccgaaag tatggccata
    35521 attcaaagca tgcctaagat ggttatcgta gggatcggca tctacatagc gaatcttgac
    35581 ttgctgagat cgccgtacga gttcctccca gagctcggct tgtatttggg tgagcgattt
    35641 tcccgaattt atgatttctt cgaggaagag aaaatgctct tcgctatcga tggcagcatg
    35701 cttgatagcc tcggccatgc cagataacag gtttcttgaa ggcagggttt ctagccagcg
    35761 aacatcgcag agaataagat tagggagatg gaaactaccg atgagatttt ttgtagtacc
    35821 tatatcgatc gcatttttcc ctccgataga tgcgtcgatc attgcaagca atgtggttgg
    35881 aacaagcaca agctcaattc cgcgcatcca tagatgagca gcaagcccga ctagatcgct
    35941 taatgaacca cctccaatag cgacaagaat tgtgtctgga ttagcatcga agtctatcat
    36001 gtaccgacag atattctcca ataccgaaaa attcttgcaa gcttccccat ctgggactct
    36061 gatccagtgg agctcttctt ttgcattcaa acccgagtca gaaaacattt ctaatgattg
    36121 cctatagaga gtttcagttg tcacaaagat ggctttttct gaaggaatga gagagaataa
    36181 ctcagaagtt tttccaaatc gtattatgct ttgatttctt tgcatgagga tttccctaat
    36241 ctacagcttc agagaaggca tcagtcttgg tgccaatagc atccgaaaca tttgtcgtat
    36301 tattagataa ggaaaggcat tctccagaag cctgcccgat aaattcgaca ttcgattcat
    36361 ctaagaaacg cttaagccct cgaagttttt ccattagaga ttcgaacatt tcagtggtaa
    36421 gttgctgatc tttatcgctc agagcggatt gaggatctat atgcacttct atttctagcc
    36481 catctactcc tgctgctact gccgccaggc tcatcggttc aataagggac agaatgcccg
    36541 ttccatgaga gggatcgacg aggattggaa gatgactttc ttggtgaaca gcaggaatca
    36601 ttgataaatc aagcgtattt cgtgtataag cctcaaaagt ccgaattccc cgctcgcata
    36661 gaattacatt gggattccct tctgcgagta tgtattcagc gcaattgagc cattcttcta
    36721 aagtggaata catacctcgt ttgagtagga ttggttttcc tgacttgccg acttcacgga
    36781 gaagcgagaa attctgcata tttcgtgcgc caatttgcaa tacatcggca tattctccta
    36841 cccaggagac atcgcgcgtg tcgacgacct cagtgactat tggcagcccc gtctcgcgtc
    36901 gtgccttatc gaggatctta aggcctttta gtccaagacc ctggaaggca tatggagaag
    36961 tccgaggctt gaaagctccc ccgcgtagca tctgtgctcc agcctttttt accgcaatag
    37021 cggtttcgat ggtttgcttc tctgtttcca ccgagcaggg tccagcaatg ataacaaggt
    37081 catcaccgac ttttacgccg tttacatcga taattgtagt tttctgccct ggattgcggg
    37141 ctgctagttt aatattcttg aacacggcga tctccttgca ttaaatgcct atctatcaaa
    37201 agacctctaa gggggttttg ccaatcatag ctacgcatag aaacagggtc aaggtttacc
    37261 aatcttcatt ttaacgtaaa aaatgaatag tattgagcca tgtattttaa tgaactcgtt
    37321 tcaatctatg acaatcagcg ccgcgaacgc aatatctatc acgatctcat gcgcgaccgc
    37381 gtacaagaaa ttctattggt agggagtctc tatgattcct ttgtcgttga atccgatgga
    37441 gtgcttacgg agcagatcta tggggaatac ttcaaactca atctgaatac aattccaagg
    37501 atcacctgtg catataccga ggaatccgcg atagaacttt ttagagatgg caggttcgac
    37561 ttggtaatca tcatggcaag cacggatttt gatatgccac taagacttgc aacctccatg
    37621 aagagtattt ggcctgatat tcctattctt cttatggtaa cagataattc aagcttagcg
    37681 atgctcgata tgagccggct tgagcttgca gcctttaata gaatctttgt ttggaatggc
    37741 tattctaaac tttttgttgg aatgatcaaa tatatcgaag attggcgaaa tgtcgaggct
    37801 gatactcaag caggacaagt aagagtagtc ctactcatcg aagattcggt taggtattat
    37861 tctcgctatc tgccagtctt atatagggta gtgcttaagc agactcaagc cctaatcgaa
    37921 ggagagcaga ttacagagat taacaagcta atgagcataa gagccaggcc aaaggtcctt
    37981 cttgccaata cttatgaaga agcgcttaag atatttgaaa aatacaagcc ttatttgctt
    38041 acagttatta ctgatattcg ctttcatcgc aacggcgaat tcgacgataa tgcgggtttt
    38101 gagtttctca aatttgttaa gcaggaattg ccagacctac caatgcttgt gcaatctagt
    38161 gagaccgatg tacgcgagaa ggctaatgcc tttggtgcaa gttttatcga caaaaattca
    38221 gaatctctgg aaatggagct tgctgccttt tttcaagcaa atctaggctt tggaaatttc
    38281 gtctttcgaa tgcccaatgg agcagagatt ggacgagcag gtaatatgaa cgaattcaca
    38341 cagagactga atgagattcc catagaaagc ctgctttacc atgctgaaca taatcatttc
    38401 tcagcttggc ttatggcgcg cggagaaatc catgttgcaa aactcatgaa gccatacaaa
    38461 atcgctgatt ttagttctcc agaagagctg cgccaattca ttgtgaacat gattgaacag
    38521 atccgctcag ctcattctag agggatagta cctaatttcg atcctgagat ggtgaagcat
    38581 cccaggtatc tgttcaagat cgccgacggt tctataggag gcaagggcag agggctttta
    38641 tttattcgtt cgtttttgga taatttcgat ttttcgcagt atgttcccgg aatagaaatc
    38701 tcaatgccga atacggcgtt tataggtata gatgaattcg agcattttct tgaatcgaat
    38761 ggcctttggt cttgggccta ctatgacaag gaggctcagg atctccgctc ggtcttcgtt
    38821 caaaagcctc tttctcagaa actcgagaat aatctaaggc agttgctatc ggtcagcaaa
    38881 gggccgctgg ccgtgcgttc aagtggtctt ttcgaagaca tgctcatggt gccattttct
    38941 ggcgtatacg atacttatat attgcctaac aatcatcccg acggcgaagt gcgcctaagg
    39001 cagctctcaa tggcagtaaa gctgatctat gcgagcctct tttcgaagga agctcgcgct
    39061 tatttcgagg ctgctaatta taatctcgaa gaagagagaa tggcagttat tatccaagaa
    39121 ttggctggaa gccagcatgg agattacttt tatcctcatg tatcaggagt tgcacaatct
    39181 ttcaattatt acccgatctc atatattaag ccgaacgatg ggctctgtat cgctgctctt
    39241 ggattgggca catatctagt aacaggagga acggcatttc gtttctgccc cagatatccg
    39301 aagctcgata tagagctgct tgagcatcca ttggagaata cacagcgctc ttttcatgct
    39361 ctttctctta gagacaataa tccggatctt cttaaaggag agatggccag cctatccgaa
    39421 ctgcctatag atgtagctga aaatgatagg tatttctcca tgttcgcttc tacctgggat
    39481 gcattggatc agcgctttgt gcctggcgtt tcagcaaatg gacatcgcat aatagatttt
    39541 gccaatatat tgaaacatga agcataccca tttgccaaag caatagacat agcgctcgac
    39601 atcgggacgc gttctatggg tacgcctgta gagatcgaat atgcactcaa ttttagcgat
    39661 aacaaacagg aaccaaccct gtatcttcta caactgaagc cgcttattca tcctgaagat
    39721 catattgaaa tcgatttgag ttccattaat aaagaagcct gttttcttct ttctcgcaac
    39781 tgcatgggca atggcagaga cctcaacatt catagcatag tctgggttga tccacggaag
    39841 tttcaacgag gggagactct tgaaatcgca gcggaaatag aagagctaga tagcttcgcg
    39901 aggaaggaaa atttcgatta tctattgatt ggtccaggaa gatggggcac tcgggataga
    39961 tggcttggag tgccagtgtc ttttgatcag atttctcatg caagggttat agttgaaatg
    40021 gatttgccgg gatttgtggt tgatagctcc ctgggatcac atttctttca caatcttaca
    40081 actatgcata taaaatatat gaagataact caaggctcgg atgaagattt tatcgatttg
    40141 gaatggctct attcattgga gcccagaata agaacaaagc attgtgttct tacagagttg
    40201 aaaaaccctc tagatatgcg ctttgatggt cggaacagca aaggcgccat tttcaagtct
    40261 tccgaggcag gcaatttaga gctaacaata aaggctggca attgaccagc atgagcattt
    40321 ccggcaatat tcacttttat aacgaggcag ataaagatga ttgatcaaag cgcagcccgc
    40381 acactgcatc cgctcgaagt caagctgatt ttagcattcg acgagggcga gataatcgat
    40441 agtcagcgag tcaaacaagt tctcggattt agagaaggtc aagagcaaca ggctttttct
    40501 tggttgcttg ccaaaggagt gctcgaagaa gcagagagaa aaaccgaaat cttctatgaa
    40561 ctcactcccc ttgggcagga atggcataaa aagggtattc ctgccaagag aattttttct
    40621 cttttgaagg ataggggtcc gatgagccta ccagaaatag ctcaatctct aggtctcgac
    40681 cagaaaactg ttggttcagc ttttggttca ctttccaaag aaggggtatg cacaatcgat
    40741 gaggcgcgcc gcgccaagct atcaaaaaat gaattccctt tatctctctt ggagattgaa
    40801 atgcttcttg cgctggcttc ttcccagggc ggttcggttt ctgagaacgc tctctccaat
    40861 tctcaaaaaa cgctgatggc gcaaattgca aagaagcgcg gcgcgcagga cagccctttc
    40921 agaatagccg ataggatcag agttttttat cgtgtcactc ctattgcgaa agaataccgt
    40981 aatgccataa gcactttagg tatgacagga gaggaattag gcgccattac tccagagatg
    41041 ctcgagaagg gcacatggaa acaaggacat ttccgacctt acaatgttca gatcccacca
    41101 gccagagtta tacccggccg ccgtaacgcc tatgtcgaat ttctggaaag cgttaaggac
    41161 aagctagtat cgctaggatt tgtggaattc gatggaaacc ttgtggaaac agaattctgg
    41221 aactcggatg cacttttcat gccacagttc cattcggcgc gcgatatcca cgatgtctac
    41281 tacatcgaag agccaacgca cgcaaggttc atcgaggaac catacctatc tcatgtagca
    41341 gcggcgcatg aaaatggcgg caatacgggc agccgtggct ggcaatatca attcgatcga
    41401 gattttacca ggcggcttat acttcgaagc caggggacgg ttttatccgc tcggcaactg
    41461 ccaaaagctg aggttcctgg caaatatttc ggtatcgtgc gatgttttcg atatgatcgc
    41521 gtggatgcca cccatctttc ggatttctat caaaccgaag gcattgtgct aggtaaaaat
    41581 gtgaacctaa ggaccttact tggattcctg gagatgtttg cggtcgaggt tgctggagcc
    41641 aaggaagtga aatatgtacc tgggtatttt cctttcacag agccttcgat cgaggttcat
    41701 ataaagcatc ctgtgctcgg atggtttgaa ttaggaggtt caggtatttt taggcctgaa
    41761 gttacaagct ctctcggtgt cgaagtgcca gtgctagctt ggggtattgg tatcgataga
    41821 atggcgctta tggcgctcgg cctcaacgat ctgcgggatc tcttttgcac tgatttagag
    41881 agcgtgagaa tgcgtcgatc ccagtacaga atacaatgag gccggagata aaccatgcca
    41941 aaaatcgaag taaacgaaga gttctttttt cagcttgcag agcaaagctg gagcaatagg
    42001 gaagatcttg agcgcgacct aaccatagcc aaggcggaat tggatgaatg ggatagcgct
    42061 cctttggaag ccagcgaacg aactatcaaa atcgagctaa acgacacaaa ccgccctgat
    42121 ctgtggacaa ccgcaggcct ggcgcgtcag ttgcgaatat accgcacaag gaattatcct
    42181 tcttatcgct tctttacatc taaggaaaag aatctcgatg catcttatcg cgtagttgtc
    42241 gaaaagaccg taagagaggt ccgcccctgg attgcttgtt ttgtcgcaaa gggcaaggct
    42301 atcagtgacg ctctattgag ggatatgatt cagactcagg aaaaactagc ttggaattat
    42361 ggccgaaaac gaaaaggtgt ttcgatagga atctatcgaa tagggcttat cgaatggcct
    42421 gttagatatt ttgggactaa gccagattcg gtgtcctttg tgccgcttca ggagaccaga
    42481 tcgatgacct tgagccagat tctcaaggag catccaaaag gtatcgagta tgcgtcgatt
    42541 ctcgagggta aacccataca tcctttgctt acagattcca ggggaagggt gctctcgtat
    42601 ccgccaataa tcaatagtgc cgatatcggc gcggtccaag ttggcgattc cgaagtcttc
    42661 atagaagtca ccggcaccga ctacccttcg gtagcgcttt ctgcatccat catggcttgc
    42721 gatttctatg atattggctt cgaaatagaa aatgtgcgtg tcgattacga atacgaaacg
    42781 ccatttggat cctctgttgt tttcccctat tattttcaaa agggagtaga gctttctctt
    42841 atcgaggcaa atcgcctgct tgggagctca atcgatgtag aagaagcgca agatgcattg
    42901 gctcgtatgg ggctgtctgc ctgttgcgaa gatggactta ctttgcatgt atctccaccc
    42961 gaataccgaa atgatttttt gcatcctgta gatgttgcag aagatctcat gataggtctt
    43021 ggtatggaaa aattcgatcc agaaaggccg acagacttca caataggaag actcactccc
    43081 atcgaggtat tctcgcgcaa ggcaaaagat ataatgatag gtctcggtta tcaggagatg
    43141 atctataact atcttggatc tggcatggac tatgccgaaa aaatgcaaat acctgctaat
    43201 cagcttgtaa aaatagccaa tcctatgacc gaaaactatg agtatgtcag gaattcgcct
    43261 attc
//
LOCUS       DAOSOB010000010        40256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1036_length_40256_cov_78.178180, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000010 DAOSOB010000000
VERSION     DAOSOB010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 40256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40256
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1036_length_40256_cov_78.178180"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..465
                     /locus_tag="PLS88_02035"
     CDS             130..465
                     /locus_tag="PLS88_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06167.1"
                     /translation="MPEKNQSTQFIMDSAYDAKSIFQFVLDRCRIPIIDHNPKRKDSR
                     EAFDAVTKEHYKKRSVVERANSHLKDWLLPEKLLVRGIKKVSFELMCGVLCLAAIKIL
                     EQFISPSLI"
     gene            complement(703..1191)
                     /locus_tag="PLS88_02040"
     CDS             complement(703..1191)
                     /locus_tag="PLS88_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU06168.1"
                     /translation="MRHSRILSEGSWYHVFAKINRGECIFDNKEIKRLFLKVVLRAKT
                     KARHPYKFEIANFCIMGNHIHFLIRPAKGESLSKIMQWILSVFAIHYNKIIQANGHVW
                     HDRFKSVIINTIRQFILVYEYISNNPIKAGLCTLSSAYQYCGEYFISKDDFRIIEKDF
                     KF"
     gene            1856..3187
                     /locus_tag="PLS88_02045"
     CDS             1856..3187
                     /locus_tag="PLS88_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU06169.1"
                     /translation="MLQILSAKESLSPSEISLKKDLESGFFIPGRGILDMKSGLAVAI
                     TVISRFLGDPGRIGNIIFLAVPDEEGSSVGMKAAKKILSDFISSHQLQMKGIINLDAA
                     VDQGDGGEGKAVFLGSVSKVLPFALFVGKPAHAGAPFDGFNPVLAASLFMREIECNSD
                     CFGTRTSFPGEEPPPPTILYSRDMRTHYDVTMPFDIFCAVNILTFEKKPEKIFENFKL
                     LVQETLNNAISLFYERKSVFSRKKNEHIAMQKFSSKVVDFSELVNKAEYVAPGILDRL
                     RKAAEKNNPEDPVLQTAKIVHELLSFAQIEGPCAIVGFAPPFYAKAELGLKRDQDFLI
                     LLKDEIAQFNREYQESIKLRPFFPGISDMSILAPSIESHSIEYIKKQSAVIQKDLDKY
                     LDNFIKAPVINIGPWGREYHQWGERVNRYYAFNVLPDLVFRICQRILIQLK"
     gene            3233..3964
                     /gene="pyrH"
                     /locus_tag="PLS88_02050"
     CDS             3233..3964
                     /gene="pyrH"
                     /locus_tag="PLS88_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOU06170.1"
                     /translation="MTIVISLGGSIVAPQEGPSGLFLYDFRNVLLSWLNREDHKVIIV
                     VGGGYAARQWQKAYKEMIEIDKSTRGDGGPILGTDVDVRQSLDRIGIAATRLNAQLIK
                     EVFSDYSRDPIVTDPSADITMNSRILIAAGWKPGFSTDYDAVILAERFHAEKILNLSN
                     VSQIYSADPKVDPNAKPLLHISFDSLLQMIGTEWDPGANVPFDPIAAAKARELGITVI
                     FASGKNLMNFADILNDKPFIGTIID"
     gene            complement(4100..5542)
                     /locus_tag="PLS88_02055"
     CDS             complement(4100..5542)
                     /locus_tag="PLS88_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOU06171.1"
                     /translation="MNKKDFPHIHFYDQDFVDIYDRTWAWIADCWTNGGSQTKISKIR
                     FFYYPSAKTLDFLEQVFSSFFLVYSNRIYPASNGLDALYALQEPDGAIRNAYDIETGV
                     PILPKDNPLGVTLPLLSWAEFNLYHKTANKKRVKEIMPALSNYYKWLEANFKMPNGLF
                     ATPLSSTGMENSPRSEARYFIDFNAAMAMNALYLSALGDILNDKDASFQYKRDYFSIK
                     TRINSKMWNPEDGFYYDLDEDEIQIRTKTLGTYWLLLAEIPNEERAEKLIAKLKDPSC
                     FGTENPFSSLSVDHPAFDKKGQGYRGSVFPWLTFMVIKGLEKYYFYELARNSAIRHLY
                     YILDSMHNSDQEANKRSNHPTVWSAYQPMNEGKAIWEEHPDWPLPNHFPSNGLSTVTL
                     IIENVIGLYISLPRKTVDWIIPNLEAMGIENLSLKRNMITILSSKSGRGWEIHMESEK
                     LYYFTINVLGKKKKTLPIPSGKCSMLIDKI"
     gene            complement(5698..7128)
                     /locus_tag="PLS88_02060"
     CDS             complement(5698..7128)
                     /locus_tag="PLS88_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOU06172.1"
                     /translation="MDLESRNQPQAILPIFHSVQLDTDLCVGCTTCIKFCPTKAIRVK
                     NGKAKIFEDRCIDCGECIRRCPKGAKKAISDPIFLMDGYDLKVALPAPSLYAQFGPKY
                     RHSDIFNALHKSGFDEVFDVAWGALVVTEMTRNLLSQTDLRPRISSACPVVVRLIQQR
                     FPSLIPNLVPILPPFEIAAREVRNRLNHLSKRIGIFFLSPCTAKVTAIKMPLGYEYSA
                     INAVFSFGKVYRLIKRALDPLYSLISPPSRLPSDSELPSIRIDDILYEPHAQPLSQHL
                     LPIIDNLDSGIGWARSDGELDALHIENAVSVDGISNVIELLEAIENGNTDSIEYVEAL
                     ACPGGCVGGPMAVENPYIARSTIRQRYRSAALVSAVPQPFLDGYQNIKEESRPPSPKS
                     IEKEYEAFRWNKPLSPNPVLVLDKDLSKALEMAERIEYIRGKLPGIDCGACGAPDCDS
                     FAEDIVRGLSTLDECILLADQDINQS"
     gene            complement(7129..7554)
                     /locus_tag="PLS88_02065"
     CDS             complement(7129..7554)
                     /locus_tag="PLS88_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU06173.1"
                     /translation="MIIEYNIPRLDFFAAGKTSTDLKHRLIQLGLPPPLIKRIAIAAY
                     EAEMNIAIHGDGGRAEIDISKDKITTRFIDHGPGIPDIELAMQEGYSTAPEEIQNMGF
                     GAGMGLPNMKKNADELIIQSEPNNGTTVVMHFNLFSGES"
     gene            complement(7593..8195)
                     /gene="recR"
                     /locus_tag="PLS88_02070"
     CDS             complement(7593..8195)
                     /gene="recR"
                     /locus_tag="PLS88_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOU06174.1"
                     /translation="MKSIEDLVSILTKLPGIGRKSALRIAYALLRSDISFSEALAESI
                     LMLRKTVRFCSECGSYAETDICQICLDNSRDRSIICVVEQPQDVFTIETSREYRGLYH
                     VLGGLLSPLEGIGPESLSINKLIQRIHNSFSTSVPIQEIIIATNPTVEGDTTALYLKK
                     LFEKELVSVTRLATGIPVGGDLEYADKLTLARSFRGRAPL"
     gene            complement(8341..8694)
                     /locus_tag="PLS88_02075"
     CDS             complement(8341..8694)
                     /locus_tag="PLS88_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOU06175.1"
                     /translation="MDLSNIIDMLKNPQALKMQAEEMQKKMNAIQATGQAGGGMVKIT
                     LSGNMELLECLISPELSDLKDIPLLQDLIRAAHHNATEKIREAIQKEFAGSLGEFSTS
                     IPFGNFPGNFTGTEV"
     gene            complement(8722..10725)
                     /gene="dnaX"
                     /locus_tag="PLS88_02080"
     CDS             complement(8722..10725)
                     /gene="dnaX"
                     /locus_tag="PLS88_02080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOU06176.1"
                     /translation="MQFEVTANRKRPQTFEQLVGQSFVSSTLISSLEQGRIAHAYLFS
                     GPRGCGKTSTARILAKALNCEQGPTAHPCGTCDSCISIANGSSLDVIEIDGASNTSVD
                     NIRQIKDEVLFPPNIGRYKIYIIDEVHMLSMSAFNALLKTIEEPPPYIAFIFATTEPH
                     KVPATIKSRCQQFNFRLISVETIFELLKQVVLETGIEAEEEALLWIAREAAGSIRDAY
                     TLFDQIASFSGTKITAQNIRETLGLVGIESLNALFRAIAKGNSQDAFLILDDIIMRGV
                     SPEQFLTDAVNYCRSVLLIMNGIQKENLIIAPHTVFEPIVIESLSKAQIEYSIALLLD
                     TYRHLKESIEPRYEIELTIAKLCHLSSYISPLELFDMLHNIQQLFKKNEIRASSLNEI
                     GSPESIIKETINSKSSDKQIPLSELRKKIISKLRSQNLFLASALEKSAEWQPKHNGII
                     IPVSNRVEMDIILRFESFIIETATQILGKPCAIEVRHLSVQENHENNENSDLRPPLQT
                     NTQSSEPQPQTSVPQNGETIQGEIDKKKTTNERTPQGQDSLLKENIGLSGEKGDSPDS
                     FELPLETNEIGFSNNDEKIKSGNGIQCSDASEQNKLTESEAHLVDVVRKVFKGTVVAT
                     KVTHEPSILEKSSLSSNLSRNTSYSSELSEQSEEFADMQDEEI"
     gene            complement(10775..10872)
                     /gene="ffs"
                     /locus_tag="PLS88_02085"
     ncRNA           complement(10775..10872)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLS88_02085"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(10893..10980)
                     /locus_tag="PLS88_02090"
     tRNA            complement(10893..10980)
                     /locus_tag="PLS88_02090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10944..10946),aa:Ser,seq:gga)
     gene            complement(11062..11147)
                     /locus_tag="PLS88_02095"
     tRNA            complement(11062..11147)
                     /locus_tag="PLS88_02095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11111..11113),aa:Ser,seq:cga)
     gene            complement(11156..11229)
                     /locus_tag="PLS88_02100"
     tRNA            complement(11156..11229)
                     /locus_tag="PLS88_02100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11193..11195),aa:Arg,seq:gcg)
     gene            complement(11239..11326)
                     /locus_tag="PLS88_02105"
     tRNA            complement(11239..11326)
                     /locus_tag="PLS88_02105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11290..11292),aa:Ser,seq:gct)
     gene            complement(11347..11431)
                     /locus_tag="PLS88_02110"
     tRNA            complement(11347..11431)
                     /locus_tag="PLS88_02110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11395..11397),aa:Ser,seq:tga)
     gene            complement(11520..12584)
                     /locus_tag="PLS88_02115"
     CDS             complement(11520..12584)
                     /locus_tag="PLS88_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU06177.1"
                     /translation="MQRSLDSIEKVRINWHSQCLKARYGEKVWRLGLDAGFTCPNRSA
                     DRLQGGCRFCAADGNISAYQKSQTSVPNSNTSVTKAINDRSTIEDQIRRALAFTRQRY
                     KAKAFFLYFQAYTCTNAPVSMLAKIYNDAIKTFMRIYYDTQKQASVDLKLSDISMTPL
                     KGIIVSTRPDCFDIEKAELLASYRKQDLEVWIEFGLQSAHEKTLAFINRGHGIDEFLI
                     AMDIAKKAQLNRTVHIILGLPGESHRDMIETIKFVAQIKPEGLKFHDLHLVKGSAFAR
                     SFHSGEITLMHPSRLPVLIADCLELLPPSTEIMRLSADFRPEETINLSPQTDKHKLAR
                     AVEEELLVRDSFQGRRFEMD"
     gene            12669..13994
                     /locus_tag="PLS88_02120"
     CDS             12669..13994
                     /locus_tag="PLS88_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU06178.1"
                     /translation="MYRRCTFRIGISIFALIIILLCSACVHFTSSALIWTDMPELIIA
                     AQLFNRENDQYTIDVEYKAYPASEINNATKPPSLIIAKYMLSNTVLKKFASLDELFTK
                     YYLDPNDIYPALLNAGKLGSRHVLLPVSFDCELIIENKSETARAGTAMLDSEMIRQAS
                     QAFTRIENGKISSLGFSPRWDLSFAENWLLAGNVGFAPNQNWKLTSVPAVSADMSLWP
                     ILWNKQNLEKSIEVLLSFNSDIRISDQDAFAFVYFNKPGYQLVLEGKVLYWPMKASEF
                     FKLPHSTREQLRYRFPTVENKLILTSDSRYMGIPKGSKNRKAALEFARWLLVSTNQEK
                     IWKEMESQQLLPDYVGAFDGFSSIMQVNETIFLRYFPDYGQNPLLSAAIPEPAHIPDY
                     WEDFSRDFLHHWLESVMSNPDSRRAIDQDFRIAFEKYIGTMPDWLISNN"
     gene            complement(14018..15064)
                     /gene="dusB"
                     /locus_tag="PLS88_02125"
     CDS             complement(14018..15064)
                     /gene="dusB"
                     /locus_tag="PLS88_02125"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOU06179.1"
                     /translation="MKKKIPFSTNLDSWNVPFFLAPVAGYSDVAFRAICFEMGAALCY
                     TEMVSAEALIRGHPKTKLLLARDPVERYFAIQLFGANPTTLAKAVQIAATYKPVVIDL
                     NCGCPVPKIIKAGAGAALLKTPKRIGEIVHAMRDQTSVPISIKLRTGWDQNSINYLET
                     AAIAEEAGVCAITLHARTRAQGYAGKADWEAIKALSEQTSVPVFGSGDVLSASAAMSM
                     IRETGCNGVMIARGAIGNPFIFSDIHELAHRRNAEAYSNKPPSLFLESPSNPESTFIY
                     RRPDIIAQTALRHLEMSTKYIGETRACIEFRKHFCAYTRGLPGGAELRAQAVRCSTID
                     EYKPLFEIITRIYS"
     gene            complement(15102..15881)
                     /locus_tag="PLS88_02130"
     CDS             complement(15102..15881)
                     /locus_tag="PLS88_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU06180.1"
                     /translation="MRYFDTHAHIGLIYDDPIEQLLVCQKAKLAGVSRIVSICNSLVD
                     FKQVYENLKPAEYVYHAVGVSPSEVNNPGKDWQRFIEEASKLLRVIAIGEIGLDYYHK
                     FGDRRSQIELFIEQLEIAARLGLPVIIHNREAGKDVLDILKDRLPPAGAVLHCYSENA
                     AYAADILDLELDIYFSFAGNLTYRNARNLHETVAFLPLDRILLESEAPFMVPAEYRNK
                     RNMPEYLPSTAQFLADFLNIDVEEVAETTYQNACRFFKLAP"
     gene            complement(16144..16875)
                     /gene="lptB"
                     /locus_tag="PLS88_02135"
     CDS             complement(16144..16875)
                     /gene="lptB"
                     /locus_tag="PLS88_02135"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOU06181.1"
                     /translation="MTNAKLEVINLRKSFGHTLAVSDVSFSMEEGEVIGLLGPNGAGK
                     TTVFYMIAGFLRPNAGSVLFNGMMINNLPMYRRAILGISYLPQESSIFRRMTVEENIL
                     AVLEARSGLSSAARREKAHALMEEFGVLHVAKQPAYTLSGGERRRTEIARALAVEPKF
                     LLLDEPFTGVDPIAIREIKLLIHNLSKKRIGILLTDHNVRDTLAITSRAYIINKGTIV
                     ANGQPQSIIENPLVRETYLGHDFEM"
     gene            complement(16872..17594)
                     /locus_tag="PLS88_02140"
     CDS             complement(16872..17594)
                     /locus_tag="PLS88_02140"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOU06182.1"
                     /translation="MKLKIIALILGLMMIFGSLSIIFAQTNPPSLQPSPEPVAQAQQT
                     QPSSGKKPIVFSANTVQGVIAKGKENTILMGSVHITTGSMIITADRVDLSGEDYVNVV
                     CLGNVTVQDTEKGFSLVAEKLNYNRDTEIGLAQKKVFVNDTKNNTIIEAEWLRFDQKQ
                     SIFEAAVTVKVRKEDMSISSEYALYNRDTEEIWLHGGAIAVTEDGVLKGDVISSSADW
                     SKLKISGEVSGTITTKETATSQ"
     gene            complement(17599..18189)
                     /gene="lptC"
                     /locus_tag="PLS88_02145"
     CDS             complement(17599..18189)
                     /gene="lptC"
                     /locus_tag="PLS88_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOU06183.1"
                     /translation="MGAYRREKWPTQSIFSLCLFCTLLILLSSCSQVAEENSIDAQED
                     IPDAQFVDIIRKEYREGKLSMQFSAKSASWYETDQRLEVEELSFITYNGDDGSVSASG
                     DAEKAVFYEVSGDAEFSGYVHLQSAEGDASFESTKLSYKRSLDVFETPHDTEVLIKVG
                     NQLLLAGNGLLFDVKQQYYHIQENVHGSVNQQDEVE"
     gene            complement(18235..19842)
                     /locus_tag="PLS88_02150"
     CDS             complement(18235..19842)
                     /locus_tag="PLS88_02150"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOU06184.1"
                     /translation="MRKLLFVTGGVCSSLGKGIAASSIGALMEARGFNVQMVKIDPYL
                     NVDAGTMSPYQHGEVYVTDDGAETDLDLGNYGRFTTAKLSRANSITTGQVYKKVIEKE
                     RTGDYLGRTVQVVPHITDEIKERILAVANNPEVDMTIVEIGGTVGDIESVPFLEACRQ
                     LIHEFGKQNAISIHVTLVPAVSSGEIKTKPTQHAVKELQEVGIQPDALILRSTKPLSD
                     ELRRKISLFTNVEEEGVVSGYDAATIYEVPLIYYNQKLDTFLLRKMNVESRHAELSRW
                     QKVVQAITKPNRKVKIAVVGKYMDLQDAYKSIWEALIHGGIAHHTGLDIERIDSSRLE
                     DPAADFDAIFKDIHGILIPGGFGERGIEGMIRAAQYARERNIPYFGICLGMQIMVIEY
                     ARAVLRWQDAHSTEFDKSTQHPVVCLLEEQRKMKNYGGTMRLGAYDAVTKAGSNIRAA
                     YGVELISERHRHRYEVNSDIRTDLEAHGLCVSATTVDGELIEACEWPNHPWSIGVQFH
                     PEFKSRPTAPHPLFKEFIRAALNHSGL"
     gene            20019..20714
                     /locus_tag="PLS88_02155"
     CDS             20019..20714
                     /locus_tag="PLS88_02155"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOU06185.1"
                     /translation="MEQGQLLARGLAALDFDISSGILDKLLYYIDELERWNPVYCFVK
                     AEGQDLIIKHVLDSLGPWRILEDLFIEMDKQFSDNKKLRITDIGTGAGFPGLPLAIAF
                     PQRSFLLIERLEKRARFLENVICILGLENVEIHQNTVEEEDEPLGCVIFRALRPFTDK
                     KLFRTIWKKIQLGGALCAYKGRIMHAKLELTGLLDDPVLSSMAAHAKITPVWVPFLEE
                     ERCLVVARKTLNI"
     gene            complement(20954..21616)
                     /locus_tag="PLS88_02160"
     CDS             complement(20954..21616)
                     /locus_tag="PLS88_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOU06186.1"
                     /translation="MNSPNMIKSPGTTFLPSPQYLPAFYYNIPSPFGDILLIWQDIDS
                     PRVNRIYLPKEGVSQISQAKSDFYLLEQWRKKVPLPQHIELILQLILNMLSGRPVQPP
                     TNIIKDSLSHLKIPEFRQKVLLMEATIPFGFISSYKELAKACGRQLSFRAVAGSLSHN
                     PLPLLIPCHRIVASNGYLAGYQGGIAMKRFLLENEGVPIRSNKVIIEKAKFWDFSINY
                     QR"
     gene            complement(21609..22724)
                     /locus_tag="PLS88_02165"
     CDS             complement(21609..22724)
                     /locus_tag="PLS88_02165"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOU06187.1"
                     /translation="MIEIEVPATCANLGPGFDCLGIALSLSNIVRLERAERTQIKGCP
                     VEWANEDNLFLRSFRHSCMLLQTSVPKISAEIITGIPPARGLGSSASLAVAGAAAALL
                     LSGKTIKSLRDLAGTVAGTGTEVDDVDLTGGTGTEANLTDCTGTEVDLTNLTSFLHEE
                     ANQYFLLRAATDIEGHADNAAPAIYGGFTAAALTEKDIVISQNPAPEHWQFMAFIPDF
                     ILETSVARNALPDSYSRQDVVHGISHAALVALAFLKADLNLLSCACSDRIHEPYRRPL
                     IPDFDIVKTACLEQGAKMICISGSGPTIMAIFETSVPKAPIIASSTTKSLNEVLEKRI
                     AEKATHNWIALPLNVDNLGIRARKIFTNNLRRHSQNE"
     gene            complement(22732..24357)
                     /gene="thrC"
                     /locus_tag="PLS88_02170"
     CDS             complement(22732..24357)
                     /gene="thrC"
                     /locus_tag="PLS88_02170"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOU06188.1"
                     /translation="MVVRYTSTRDAKVDVNIAEAIVQGMPQDGGLFVPRNIPTLSPEA
                     ILEPKLSYAELAWLVLSPWFDWSETELRPLIEKAYSSSGKKSMFDTPEIVLLSNAGSP
                     NCENDLAGKKESMQLFLLELFHGRTCAFKDLALSILGGLLSKSLEKCGIDEPVLVLTA
                     TSGDTGSAALAGLGGQQGIRIAVIYPAEGTSEIQKLQMTSVPLEDRCVIGLKGTFDDA
                     QRAVKNIFQSAKDPRSPFFGIRLSSANSINIGRLLPQIVYYVKAWRDLYSAGAINCGE
                     SFDVVVPSGNFGDILAARYAKAMGLPIRRLVCASNSNRILYDFFATGIYDKRRAFNKT
                     LSPSMDILVSSNLERLLYHAFGENSELVLRLMHRFENEGFFQIPEQVASNLSDFYTGW
                     ADDFQTLESIRHMWEIHNLLVDPHTAVACKVAHEVFVNSQYHQKDRKIIEAQAINPIV
                     IAATASPFKFPASCLAALQSNLKNSPIAHNELSLAFELSSYTGISVPKPIADLVTAKI
                     NHNMIVEESNLESVLADFANKTAKPQVYRKSTM"
     gene            complement(24468..24986)
                     /locus_tag="PLS88_02175"
     CDS             complement(24468..24986)
                     /locus_tag="PLS88_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOU06189.1"
                     /translation="MLSQKMIDRINLQINREMYSAYLYLAMAAKMNELGYTGVGKWLT
                     AQYHEEMFHAMKFAEYLHDQNAVVSYTKIDSPEFKENEIKPLFQHVLSHEKSLTASIR
                     EIMDMAIAEKDYATQILAQWYIDEQVEEEKNATEIIQKIDLLGNSPQGLYLLDAELGK
                     RESSAPLDFNKI"
     gene            complement(25092..25661)
                     /locus_tag="PLS88_02180"
     CDS             complement(25092..25661)
                     /locus_tag="PLS88_02180"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOU06190.1"
                     /translation="MIRCPWCGNDPVYIRYHDEEWGSPVHDDQTHFEFLLLETQQAGL
                     SWRTILGKREAYQKAFVNFEPEIVASFCEKDITSLLSDPGLIRNRRKLEAAVKNARAF
                     CDIKKKYGSFDAWIWHFVEGRPIVNHWQDISEIPTQTELSDLVSNEMKKAGFSFVGSI
                     TVYAHLQAIGIINDHLITCFRHAEIANLR"
     gene            25792..26325
                     /locus_tag="PLS88_02185"
     CDS             25792..26325
                     /locus_tag="PLS88_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06191.1"
                     /translation="MRKSKIVTIAFMMIVAILSPLLAQNVTKEVHAEAGELHALGGIG
                     FGWRGFGISGGMEYMLQKLNIPNFPLSLGVMGIAGMDFGKGFDLSAATMATLHWSMKA
                     YKDFPKFFKNFDWYLGLGMGVGIVSAFGFGISTGGGLSYYVNDKFAIDLHSYYINYFV
                     GGGSGFSESIGIRYTLK"
     gene            complement(26582..28702)
                     /locus_tag="PLS88_02190"
     CDS             complement(26582..28702)
                     /locus_tag="PLS88_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU06192.1"
                     /translation="MKPIPNDYRIRSQMPLILLSLLLICLSTSNLQAQQDSPFPLELS
                     SLNNFEYAFPEGDTLRNITKPVFLGEETFIARLKQRAESLEFDQSKDNEPRPPLGIVL
                     CGGSARAYAHIGVLKTLEKAGIYPDFIVASSMGAIVGMLYAAGYSPQDIEFLIQALPL
                     ESFFTPIIPTNGGLINTEMFRAILRKIIGRLDVSETAIPIIITAEDLKSRQQIWIAEG
                     PFDLVMASAFAMPAVFEPQAFDQFILIDAGATTIAPVEPALQFSNRLVISTAFYNRAM
                     NFSSPVTVINRSVDIGKTRAGMAGIERSKALVIRNDVENLSYMQFTDPDIIIKKGEIS
                     AQNALEQLDLISRKELENAPPSGLLELRSKMHENLNKTIRDLQSGQQPSTSFTLRGIP
                     ILRLFDPFTLMPGETGTEARIGASLSFSYSKFKSDLSYFNALYPDPGKVWAIETALRV
                     NPIDSLNVWLTTRLWGDYTPTSILSHNTEYWEIAGRTSLMKVYEEKKIGFQAGGDIFL
                     KMDFSIANWQTCAFFHTAIPRKYKKFGPPIQPWYTANLGGFAENSVGLQNAAGLYGSL
                     QGGFDSKWVSPKFRAFSKVSLNNQEFMESKYDGFRSSAARKVVTSNLITNVEIPFSPH
                     ELYLDISEALLLKGFELAPFFDTRWNSTSDESFRIYDWAAGLSFSFEARAFGLAPATM
                     TFYASYSGSKIITLQFRAGMLLPE"
     gene            29007..30293
                     /locus_tag="PLS88_02195"
     CDS             29007..30293
                     /locus_tag="PLS88_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU06193.1"
                     /translation="MISEILVLLALILINGFFSLSETALVSSRKARLKAEADKGKSTY
                     KLALNTRESPSRYLSTIQVAITLIGILIGTISGTTFSQLLARWLSRWSFIGRYADSIA
                     VAIVVLAITFLSVVIGELVPKSIALRNPEYIAALTIKPMHALSIVFYPIVRFLSSVTD
                     SLTKLLRFSHKAETAITPEEVRILLEQGEKSGVFEAAEREMMEEVLSLDDRRVTMFMT
                     PRTEVVTLNVKDSRDSHIRAIIKNSEYGYLPVVDGDLDHTIGMVSVKRALTCMAEGCF
                     LSLETCMEPAVMIPESLSGLQALAILRDAKKSAGLIVDEFGGISGIVTIADLLEAIAN
                     LSEIETEEAPQILKRSDGSYLVDGSMPIDEFAENLGINLDIISSEDYDTVAGFVLHCG
                     GSIPKAGDIIEWPPLRIEIVDMDGKRIDKVLVQKSD"
     gene            complement(30367..30822)
                     /locus_tag="PLS88_02200"
     CDS             complement(30367..30822)
                     /locus_tag="PLS88_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOU06194.1"
                     /translation="MNQKLRKITKNKQKTLSWLRSALTFSKAQISAFVGGVIDYCTMI
                     FLTEAFHIHYTISIAAGGIVGAVVNFSVNRKWSFYSNDAQYEFSVLQQLGMFFPVVLG
                     SIALKSAGTFALTSLSKIDYKLTRLVVDVVVSIFYNFMLQKHLIFKKRR"
     gene            complement(30788..31534)
                     /locus_tag="PLS88_02205"
     CDS             complement(30788..31534)
                     /locus_tag="PLS88_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06195.1"
                     /translation="MDKGNAKIGMKQKKNSIDHTLPIISHDRIRTNLLKKQEQKALAF
                     LVQYIPSWMSSNMLTAIGFLGNVIVFLSFILAELFDTRFLLIGVAGFVISWFGDSLDG
                     RVAYYRKKPRKWYGFMLDLVVDWLGIVLIGLGFAIYANSFQKILGYAFVVLYGLEIMI
                     ALLRYKIGGSYSIDAGKLSPTETRILISLFLVIEVLFNGSIVYLAAIADIILAISNFS
                     EFKKLAKVANERDEEENRKGESKIAENNKE"
     gene            31683..32369
                     /locus_tag="PLS88_02210"
     CDS             31683..32369
                     /locus_tag="PLS88_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06196.1"
                     /translation="MNKKRLLYIAIYAIGMAYIEASIVVYLRKLYGISDLMISVPSFD
                     PQVSIIELGRELATLIMLFAIGFASGKRFQSQIGFSLYAFGLWDIWYYIWLVVFIGFP
                     HSFFDWDLLFLIPLPWWGPVITPVMISVLMIISGARLALLEERGLLVKPRSVDWIMLS
                     IGACIMLYTFMSNAIGSLPTSVDNLSDLRPSSFLWSIFLVGYVIATYATWRILYKSLD
                     EPAMHRSVRK"
     gene            complement(32319..32903)
                     /gene="pdxT"
                     /locus_tag="PLS88_02215"
     CDS             complement(32319..32903)
                     /gene="pdxT"
                     /locus_tag="PLS88_02215"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOU06197.1"
                     /translation="MSSHRIGLLALQGDYEAHGKALERVGFPRCDIVEVRRFSDLESL
                     TALIIPGGESTVMSMLLERFGMFDELKRRIVNGMPILATCAGLILLAKDIVGNNQMSL
                     GLLDIRVKRNAYGRQVDSFHAPVQTIISSIGKIEGIFIRAPRILASGKEVEVLAMYQD
                     EPVMIRQGSIVAATFHPELLPDAPVHRWFIQTFI"
     gene            complement(32900..33805)
                     /gene="pdxS"
                     /locus_tag="PLS88_02220"
     CDS             complement(32900..33805)
                     /gene="pdxS"
                     /locus_tag="PLS88_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOU06198.1"
                     /translation="MEQKLWSDRQSEQWHIKVGLAEMLRGGVIMDVTNAAQAKIAEKA
                     GACAVMALERVPADIRVQGGVARMSDPKIIKEIQDAVSIPVMAKCRIGHFVEAQILEA
                     LGVDFIDESEVLTPADDRFHVYKHSFKVPFVCGCRDLGEALRRIGEGAAMIRTKGEAG
                     TGDIIEAVRHMRTVRDGIARLIGLPEEELMTEAKELGAPFELVWEVAKTGKLPVPNFS
                     AGGVATPADAALMMQLGAESVFVGSGIFKSSNPERRAKAIVSAVVHYKDPKILAEISE
                     DLGEPMVGIGVDDLAENQKISERGW"
     gene            complement(34010..35428)
                     /locus_tag="PLS88_02225"
     CDS             complement(34010..35428)
                     /locus_tag="PLS88_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOU06199.1"
                     /translation="MKYNPVSKDIISELINIAGERNVLLDPAQLEIFSHDETSKEEYG
                     HMPEVVVTPRSTSAVAEIVKLANRTSVPITPRGAGSGLSGGAIPIFGGIVLSLEKMNQ
                     VREIDYDNLTMTVETGIVTNEINNLVKEKGLFYAGYPMSLETCMLGGNIAENAGGGKA
                     IKYGVTSRYILGLEFVTPTGEVVWLGGKLAKDVTGYDLVHLIVGSEGTLGIVTAAIIR
                     LIGLPAAKSDLLVLYRSPQEAISSVPVILSKGLIPTSIEFMDRRSVETSCAYLNENLP
                     YAQCGAMLLIEMDGRDQSIVESEAEAVGDLCMEQGAIEVYVADNRTTQERLWAIRRNI
                     AEALKVYCPVQSLEDIVVPPAAISAVMPELERISSQFGIEIPCYGHAGDGNLHATLVK
                     DPQMPMEQWQRIEQQALLALYESITALGGKISGEHGIGLKRKDFMKRFMSPVELNLIK
                     AIKRAWDPNLIMNPGKIFDIEY"
     gene            complement(35418..37625)
                     /locus_tag="PLS88_02230"
     CDS             complement(35418..37625)
                     /locus_tag="PLS88_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOU06200.1"
                     /translation="MHIIVLIKQVPETDKVKMDPETGTMVRSGLESIINPLDLYAIEE
                     ALMLKEHFGAHITVISMGPLDAERALRESLAMGCDKAILLTDKAFAGSDTWATSRVLA
                     ETIKHIGTFDLILAGERATDGDTGQVGPAVASWLGIPALTYVSSLEIESRTKDSRIAE
                     SDHNRTSSLTFLVRRLTEEGYQKVRVQSPCLITVVKEIASPRLPTLRGKKFSRSAEIS
                     HWGAKEINLDPASVGLAGSPTRVVKIFYPKVARGGERILALDEAAIDKAIDRILSTIG
                     PIMGDGGCSGIHKVANIEKSETKVSLAKSQPAMQESMISLSEQLSSGPIVDDKPEFWI
                     ITERRKGGIDLVSLELLARARHLADSRGATLVAIVLGPSSKDEAAMLIAHGADSVILV
                     ESPSLEDFICELWAEALFELACARKPEVILGAATTTGRTLMPYLAAKLGTGLTADCTE
                     LSIEEGSGLLLQTRPAIGGNIMATIKTAHHRPQMATVRPHSMQPLPPDFGRRGSIIHV
                     IDDSSLGKIISDSLALSVQVLALESNVKDFENLESAQIVVSGGRGLKKADNFKLIREL
                     ASSLGGAVGASRESVDRGWISYPHQVGLSGKTISPEIYICAGISGAVQHLAGIRTAKK
                     IISINTDPDAPIHAVADLAIIGDLFEILPRLTAQLKSIYSSSSGSESKPEPRLDPGFS
                     PGSVLRPGSVSSLGHGSSSPSSSLSPCQTKDTKLQEPDLPRSEILSTEAQHEI"
     gene            complement(37802..38140)
                     /locus_tag="PLS88_02235"
     CDS             complement(37802..38140)
                     /locus_tag="PLS88_02235"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOU06201.1"
                     /translation="MRVFYIVLLIAVAILAVIFAVQNSVPITVSFFGWSSQASMSIVL
                     VLTFAAGILIGMLLLFPSIWKRMRELSAQKKKTNEAKKLVKETQEAQALSSSQAGQTD
                     TSSTAQDIKA"
     assembly_gap    38247..38346
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<38347..40172)
                     /locus_tag="PLS88_02240"
     CDS             complement(<38347..40172)
                     /locus_tag="PLS88_02240"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOU06202.1"
                     /translation="MSPAYVKNEKLLSWVSSMEELCKPDLVYWCDGSDEEYETICRSL
                     VASGTFIPLNPEKRPNSFLARSDPSDVARVEDRTFICSKDKSDAGPTNNWVEPNEMRG
                     ILNKIFAGCMRGRTMYVIPFSMGPVGSPFSYIGIEITDSAYVVANMRIMTRMGRKVIE
                     ALGENGEFVPCMHSVGMPLEPGQKDVQWPCNRQTKYIVHFPETREIWSYGSGYGGNAL
                     LGKKCFALRIASVIARDQGWLAEHMLILGLESPSHEKTYVAAAFPSACGKTNFAMLIP
                     PEPFEGWKVTTVGDDIAWVRPGKDGQMRAINPESGFFGVAPGTSEKSNPNAMATIRTN
                     TIFTNCALTPDGDIWWEGMTKNPPAGLIDWQGKPWDPASGKPAAHPNARFTAPIQQCP
                     SIDPAWDSHEGVPIKAFIFGGRRSTVYPLVYQSFNWVYGVYLAATLGSETTAASTNAI
                     GLVRRDPFAMLPFCGYNMASYFDYWLHVGLSNPNPPLIFGVNWFRKDADGNFIWPGFG
                     ENIRVLKWIVDRANHKANAIESPIGWMPRYEDLDWEGLDFSKERFIELMSVDRELWAN
                     EILSHEELFVKLYERLPKELIFVRQLLLSSLWRAPEHWKSDT"
BASE COUNT        11013 a         8368 c         8833 g        11942 t
ORIGIN      
        1 caagtttctc taggtatatg agctatttag cagacaatgc ttctctttaa gagagcctag
       61 ggaagatggt gaagaactac tataaggata aatcagtgga ttctgtggcg agaggttcga
      121 cagcaataag tgccagagaa aaaccagtca acgcaattta ttatggacag tgcttatgat
      181 gcgaagagta tctttcagtt tgttctcgac agatgccgga ttccaatcat cgatcataat
      241 ccaaaaagga aagactcacg agaggctttt gatgcagtaa ccaaggaaca ctataagaag
      301 cgctcagttg tagagcgagc gaattcgcac cttaaggatt ggctcttacc ggagaagtta
      361 ctagtgcgtg gtatcaagaa ggttagtttt gaactcatgt gtggagtgct ctgcctggca
      421 gcaatcaaga ttcttgagca gttcatttca cccagtctca tctaaagacg aatagcataa
      481 accatagaag ctctcatgac ccatcgaaga gagagctata agcttgtatc tcatagaaaa
      541 gtctcttcaa agcccttttt cttacttttt tgtactcgtt aatccttgct tcatgattta
      601 tcctgcaaaa tcctgtctct attcttattc ctacaaccta tgccagaata ttcgtggacc
      661 tttgaagagg gtcagaagag agctttttaa ggcttatttt gattaaaact tgaaatcttt
      721 ctctataatc cgaaaatcat cttttgatat aaaatattca ccacagtatt gatatgctga
      781 agataatgtg caaaggcctg cttttatagg gttgttgctt atatattcat atactaaaat
      841 aaattgcctg atggtattaa ttattacgct cttaaaacgg tcatgccata catgaccatt
      901 tgcttgtatt attttgttat aatgtatagc aaatactgac aatatccatt gcattatttt
      961 tgatagagat tctccttttg caggcctgat taaaaaatga atgtggttgc ccataataca
     1021 aaaattggca atttcaaatt tataaggatg ccttgcttta gtttttgcac gcagaactac
     1081 cttgaggaat agtcgcttta tctctttatt gtcaaatatg cattctcctc tattgatctt
     1141 ggcaaaaaca tggtaccagg atccttctga taatatcctt gaatgtctca tataaagaac
     1201 atcgcagaaa aaagaaaaaa taattcaaag tttggaatac aatcactttg agattaacta
     1261 taaactgcat tgctaattct agagtaaaat agggagacta aaatattgaa gtacaaagct
     1321 gaaacattta gccagtatta aatctttaaa tgctaataag ttagatatag tgccaatgct
     1381 gtatgccaaa cctaagccaa acatacacca aacctccgtc cccttgttat ttggatcttt
     1441 atgacttttt attacgtcta ggaatgtcat cattttttct aaatactcaa ctaagctttt
     1501 aaatttatga tagagtagca tctatatgat aaatagaaac agtaatatag atactcagtc
     1561 tctcgcgatt cgattggcca aatttgctag cataacgaaa acctcaggag aattatccta
     1621 tcctgatttc cttatttctg ttcttctaga aatacccttc tttagtaaaa ggccaaacct
     1681 tgtacaagaa tttgagattt ctgaggatga gtggaaacgg agaattgtaa ttgcttttgc
     1741 tccaggagaa gggcaagcat gtataacgct tacaggacat tatgatgtag ttgatacaag
     1801 caattatgga gccttagagc cgcttgcttt tgatccagat acacttttga aaaaaatgct
     1861 acaaatactt tctgctaaag agagcctttc gccttcagaa atatctctaa aaaaggattt
     1921 ggaaagcggt ttcttcattc cgggcagggg catcctagat atgaaaagtg gactagctgt
     1981 tgcaattact gtgatctcta gattcttggg agatcctggt aggattggga atatcatttt
     2041 cttagctgtt ccagatgaag agggatcttc tgttggtatg aaagcagcta agaagattct
     2101 ctcagatttc attagttctc atcagcttca gatgaaagga ataattaatt tagatgctgc
     2161 agtcgatcaa ggggacggag gtgaaggcaa agctgtattt ctcggatcag ttagtaaagt
     2221 tcttccattt gctctatttg ttggaaaacc agctcacgca ggagcacctt ttgatggctt
     2281 taacccggtg ttagctgctt ctctttttat gagagaaata gaatgcaata gtgactgctt
     2341 tggtactcgt actagctttc ccggagaaga accgcctccg ccaactattc tctacagccg
     2401 agatatgcgg acacattatg atgtaactat gccttttgac atattctgcg ctgtgaacat
     2461 tttgaccttt gaaaagaaac ccgaaaaaat atttgaaaat ttcaaattgc ttgtgcaaga
     2521 gacactaaat aatgctattt ctctctttta tgaaagaaaa tctgtttttt ccagaaaaaa
     2581 gaatgaacat attgcaatgc aaaagttctc ttccaaagtt gtagattttt ctgaattagt
     2641 taataaagct gaatatgttg caccaggtat tttagatcgt ctaagaaagg cagctgaaaa
     2701 gaacaatcca gaagatcctg tgctgcaaac agctaaaatt gttcatgaat tgctttcatt
     2761 tgcccaaatt gaaggaccct gtgcaattgt tggctttgct cctccttttt atgcaaaagc
     2821 tgaattaggt ttaaaaagag accaagattt cttaatactg ctcaaagatg agattgcaca
     2881 gttcaatagg gaatatcaag aatcaataaa gctccgtccc tttttccctg gtatttcgga
     2941 tatgagtatt ctagcaccat caatagaaag tcattcgata gaatacataa aaaagcaatc
     3001 ggcggtaatt cagaaggatt tggacaagta tctggataat tttattaagg cacctgtaat
     3061 taatattggg ccatggggta gggaatatca tcaatgggga gaaagagtaa atcgctacta
     3121 tgctttcaat gttttgccag atttggtatt tagaatttgc caaagaatac taatacagct
     3181 taaataatct tttatgaaat tataagttat ataaatacta aggagaataa cgatgactat
     3241 tgtaatatct ttaggcggtt caatagttgc tccacaagaa ggtcccagcg gcctattctt
     3301 atatgatttc aggaacgtgt tactctcctg gctaaataga gaggatcaca aggtgatcat
     3361 agtggttggt ggaggttatg cagctcgtca gtggcaaaag gcctataagg aaatgattga
     3421 aatagataag tcaactagag gggacggagg accaatctta ggcacagatg ttgatgttcg
     3481 ccaatctctt gatagaatag gcattgcagc aacaaggctc aatgcacagt taattaagga
     3541 ggttttttct gactatagtc gagatccaat agtaacagat ccttccgccg acattacaat
     3601 gaacagcaga atactgattg ctgctggctg gaagccaggg ttttctacag actatgatgc
     3661 tgttattctt gctgagcgct ttcatgcaga aaagatattg aatctttcaa atgtttctca
     3721 gatttatagc gctgatccaa aagtagatcc taatgcaaag ccacttcttc atatatcttt
     3781 tgattcgctt ttacagatga ttgggactga atgggatcct ggggcaaatg tacctttcga
     3841 tccaattgca gcagccaaag ctcgggaact cggtattacc gtcatatttg catcagggaa
     3901 gaacttgatg aatttcgctg atattctcaa tgataaacca tttattggga caataattga
     3961 ttgaatttat cgtgctttac attggtcagc ttgaagagat actcgaattt ggaaatttcc
     4021 tttaaattcc aaatttcttt tttaacattt cgtatcattc tcgactccaa gatctaaagt
     4081 tagcgcaagg cttgttttcc tatatcttgt caatgagcat tgagcatttt ccagacggta
     4141 tcgggagagt tttcttcttt ttccccaata cattgatggt aaaataatat agtttctcgc
     4201 tttccatatg gatttcccat cctcttcctg atttgcttga aagaatggtg atcatattgc
     4261 gcttaagaga gaggttttca atacccattg cttcaagatt gggaattatc caatcgaccg
     4321 tttttctggg cagagatata tatagcccaa tgacattttc tattatcaag gtaaccgtgc
     4381 taaggccatt agatgggaaa tgatttggca gaggccaatc tggatgttct tcccaaatgg
     4441 ctttcccttc attcataggc tgataagcgc tccacacggt aggatggttg ctgcgcttgt
     4501 tcgcttcctg atcgctgtta tgcatggaat ccaaaatata atagagatgc cttatggctg
     4561 agtttctagc aagttcataa aaataatatt tttccagtcc cttgatgacc atgaatgtga
     4621 gccatgggaa aacactccct cgatatccct gacccttttt atcaaaggca gggtgatcga
     4681 ccgaaagcga tgagaagggg ttttcagtac caaaacagga agggtctttt agcttggcaa
     4741 ttagtttttc tgcgcgctct tcatttggta tttctgccaa aagaagccaa tatgtcccta
     4801 aggtctttgt ccgaatctga atttcatctt catctaggtc ataatagaaa ccatcttctg
     4861 gattccacat tttcgaattg attcttgttt ttatagaaaa atagtctctt ttatattgaa
     4921 aagaagcgtc cttatcattc agaatgtctc ccaaagcaga taaatatagg gcattcatag
     4981 ccatggctgc gttgaagtct ataaaatatc tagcctctga acgcggagaa ttttccatgc
     5041 ctgtagaaga aagaggagta gcaaaaagcc cgttcggcat tttgaaatta gcctcaagcc
     5101 atttgtaata attgctcagt gctggcatta tttcttttac tctcttctta ttggctgtct
     5161 tatggtagag attaaactct gcccatgaga gcaggggaag tgttacgcca agaggattat
     5221 ctttggggag aataggaact cctgtctcta tatcataggc attgcgaatc gcaccatcgg
     5281 gctcttgaag cgcataaagt gcatctagtc catttgatgc tgggtaaatc cgattgctat
     5341 atactagaaa gaaactggaa aagacttgct ccagaaaatc aagagtcttc gctgaagggt
     5401 aataaaagaa tctgattttg ctgattttgg tctggctacc tccatttgtc cagcaatcag
     5461 caatccatgc ccatgttcga tcataaatat cgacaaaatc ctggtcatag aagtgaatat
     5521 gaggaaaatc ctttttattc aaaaaactta ccctcttatt atatgtatgt aattcgctga
     5581 atttatggat aacagcaaaa ttttataaga aattgcacta tattcaagca tatcgcaact
     5641 tactatactc attccatgtc tatttgtcaa atttatcgat tattagatac tgttgattca
     5701 tgattgattg atatcttgat cagcgagaag aatacattcg tccagagtgc tcaagccacg
     5761 aacaatatcc tctgcgaatg aatcacaatc tggtgcgcca catgctccgc aatcgattcc
     5821 tggaagtttg cctcttatat attcgattct ttctgccatt tcgagagctt tcgataggtc
     5881 cttatctagg acaaggacag ggtttgggga cagaggtttg ttccatctaa atgcttcata
     5941 ttctttttct atagattttg gggacggagg tctagattct tctttaatat tttgatagcc
     6001 atccaagaaa ggctggggaa cagcagaaac aagggcagca gagcggtatc gttggcgaat
     6061 tgtcgagcgg gcaatatatg gattttctac tgccatcggt ccacctacac agccaccagg
     6121 acaagcaagt gcttcgacat attcaataga gtcagtattg ccattttcga tagcttccaa
     6181 tagctctatg acattagaaa tgccatcgac agagactgca ttttctatat ggagcgcatc
     6241 tagttctcca tctgatcgtg cccaacctat tcccgaatcc aaattatcta ttataggtaa
     6301 tagatgttga gataggggct gtgcatgcgg ctcatataag atatcatcaa ttcttataga
     6361 aggtaattct gaatcagatg gcaaacgaga tgggggcgaa attaatgagt acagaggatc
     6421 gagcgcgcgc ttgatcagcc tatatacttt gccaaacgaa aacactgcat tgattgcaga
     6481 gtattcatat cctaatggca tcttgattgc ggtcactttt gctgtacatg gactaaggaa
     6541 aaaaatcccg attctctttg atagatgatt taatctgttt cgcacttctc tagctgctat
     6601 ttcaaaaggc ggaagaatag gcacgagatt cgggatcaag cttggaaatc tctgttggat
     6661 aagtcgtaca acgactgggc aagcgcttga aatgcgaggc cttaggtctg tctgggagag
     6721 caaattgcga gtcatctctg ttacgacaag tgctccccat gcaacgtcaa aaacctcatc
     6781 gaagccagat ttatgaagtg cattgaaaat atctgaatga cgatattttg gaccaaattg
     6841 agcgtaaagg gacggagctg gtaaagcgac cttgagatca taaccatcca ttaaaaaaat
     6901 cgggtcagaa attgcctttt ttgctccttt aggacatcgg cggatacatt caccacagtc
     6961 tatacagcga tcttcaaata tttttgcttt tccatttttg acacgaatag ctttggttgg
     7021 gcaaaatttt atacaggtgg tgcagccaac acacaggtct gtatcaagtt gaacagaatg
     7081 aaaaatcgga agaatagctt gaggttgatt cctagattcc agatccactt atgactctcc
     7141 agaaaataag ttgaaatgca ttactacagt tgtgccatta tttggctcgc tttgaatgat
     7201 tagctcatct gcattctttt tcatattagg caatcccatt ccagcaccaa atcccatatt
     7261 ttggatctct tcaggagcag ttgaataccc ttcttgcatt gcaagctcga tatctgggat
     7321 acctgggcca tggtcgataa agcgggtagt aatcttatct tttgaaatat caatttctgc
     7381 tcgacctccg tccccgtgga ttgcaatatt catttctgct tcgtaggccg cgatggcaat
     7441 tctcttaatc aatggaggcg gtaaaccgag ctgtatcaat cgatgtttga gatctgttga
     7501 agtcttgcct gcagcaaaga aatccaatcg aggaatatta tactctataa tcacaatatt
     7561 taatccctat atttaatatc ttctctcacg aactaaagag gcgctcttcc tcggaaggat
     7621 cgagcaagag taagtttatc tgcatactca agatcacccc ctacaggaat accagtagct
     7681 aatcgcgtaa ctgacacaag ctctttttca aacaattttt ttaaatatag ggcagtagta
     7741 tctccctcga ctgttggatt tgttgcaata attatttcct gtatggggac ggaggttgaa
     7801 aaagaattgt gtatgcgctg gataagcttg tttatgctta gagactcagg accaatacct
     7861 tccaaaggag aaagtagccc tcccagaaca tgatagaggc ctctatattc cctggatgtt
     7921 tcaattgtaa atacatcttg tggttgttct acgacacaga taatacttcg atcgcgggaa
     7981 ttatctaaac agatttggca aatatcagtt tctgcataag aaccacattc ggagcaaaaa
     8041 cgcacagttt tacgcagcat taaaatactc tcagcaaggg cttcgctgaa agagatatca
     8101 gatcttaata gagcatatgc aatcctcaga gcgctttttc gtccaattcc aggcagtttt
     8161 gtgagaattg aaacgagatc ttcgattgat ttcataatca tgtccttttt gcttatccat
     8221 ctttcaagtt tcaaaaacca tcaagctccg tccccaaagt ccaccaaagc ccagattaag
     8281 aaattaagaa gcgggcgata gagaaagcag cgcatccaat tcaagcagaa gcttagaagc
     8341 ttaaacctcc gtccccgtga aattgccggg gaagtttcca aatgggatgc tcgtactaaa
     8401 ttctcctaat gagccagcaa attctttttg gatagcttct cttatctttt ccgtagcatt
     8461 atgatgggca gcccgaatga gatcctgaag caaaggtata tcttttaaat ctgaaagctc
     8521 aggagaaatt aggcattcaa gcaattccat gtttcctgaa agagtaattt tgaccatgcc
     8581 gcctccggcc tgtcctgtag cttgaatagc gttcattttt ttttgcattt cttcagcttg
     8641 catttttaat gcttgtggat tctttagcat gtcgataata ttcgaaagat ccatgtaacc
     8701 tccagcttat tccttgtaaa tctagatttc ttcatcctgc atatcagcga attcttctga
     8761 ttgttcagag agttcagatg aatatgaggt atttcttgat aaattagaag ataaggacga
     8821 tttttcgaga atagaaggct catgggtaac ttttgttgcc accactgtac ccttgaagac
     8881 cttgcgcaca acatctacaa gatgagcctc tgattctgta agcttgtttt gttctgaggc
     8941 atctgagcat tgaatcccat taccgctttt tatcttctca tcattattag aaaatcctat
     9001 ttcattggtc tctaaaggaa gctcaaaaga gtcggggcta tcccccttct cacctgataa
     9061 ccctatgttt tctttcagca aagaatcttg tccttgagga gtcctttcat tagtagtttt
     9121 tttcttgtca atttctcctt gaatagtctc tccgttttga gggacggagg tctgtggttg
     9181 tggttcactt gattgggtgt ttgtctgtaa cgggggacgg aggtcgctat tttcattatt
     9241 ttcgtggttt tcttgaactg ataaatgtcg gacttcgatt gcacatggtt ttcctagaat
     9301 ttgggtagct gtctctataa tgaaagattc aaatcgcaaa atgatgtcca tctcaacccg
     9361 atttgagact gggatgatta tgccattgtg tttaggttgc cattctgctg atttttctag
     9421 agccgaagcc aggaagaggt tttggcttcg caatttgctg atgattttct ttcttaattc
     9481 ggataacggt atttgtttat cagaagattt gctattgatc gtttctttaa ttattgactc
     9541 gggagatcca atttcgttca agcttgaagc gcgtatttcg ttttttttaa agagctgctg
     9601 aatgttatga agcatgtcaa ataattcaag agggctaata tatgatgaga ggtggcacaa
     9661 tttggcaatg gtcaattcga tttcgtatcg tggttctatg ctttctttga gatgtcgata
     9721 tgtatcaaga agtagagcaa tagaatattc gatttgggct ttcgaaagag actcaataac
     9781 tataggctcg aatactgtat gaggggcaat tattaggttt tctttttgga tgccgttcat
     9841 tataagcaac acagaacgac aataattcac agcatctgtc aagaattgtt caggagaaac
     9901 accccgcata ataatatcat ctaggataag aaatgcatcc tgggagtttc cttttgcaat
     9961 ggctcgaaaa agtgcgttaa gagattcaat tcctactaat ccgagcgttt ctctaatatt
    10021 ctgagcagtg atttttgtgc cagaaaatga agcaatttga tcgaaaaggg tataagcatc
    10081 gcgtatagag cctgcagctt ctcgagctat ccataatagc gcttcttcct ctgcttcgat
    10141 gcctgtctct aaaacgactt gcttcaaaag ctcaaaaata gtctctacag aaatcagtct
    10201 gaaattgaat tgctggcatc ggctttttat agttgcagga accttgtggg gttctgtggt
    10261 tgcaaatatg aatgcaatat atggcggagg ttcttcaatg gtcttaagga gcgcattaaa
    10321 ggcactcatt gatagcatat gaacttcgtc tataatatag attttataac gtccaatatt
    10381 aggagggaac agtacttcat ccttgatctg tcgaatatta tctacagatg tattcgatgc
    10441 gccatctatt tcgataacat cgagagaaga accgttggca atggaaatgc aagaatcaca
    10501 tgtcccgcat ggatgagctg taggaccttg ttcgcaattt agcgctttag ctagtatgcg
    10561 agctgtactg gtctttccac atcctctcgg tccagagaat agataggcat gtgctatgcg
    10621 tccttgttct agcgatgaaa taagcgtaga ggatacaaag ctttgaccta ctagttgttc
    10681 gaaagtctgt ggtcgtttac ggtttgccgt gacttcaaat tgcataatgg aaaaatacca
    10741 tgcgagggtg taagggtaaa gcagtcaaag cgcataaggc caggcgctct gcggcaccgc
    10801 acctgctgtg taccgttgct tccttccgga cctggcgggt ttagacagtg gttacgagca
    10861 cagtgcctgg ccttatgcgc tcgaattatt cacggagaga gagggatttg aaccctcggt
    10921 accctttcgg ggtacacacg acttccaatc gtgcaccttc gaccgctcgg tcatctctcc
    10981 agactgcaaa aagcaatcaa taaatcaaca caacaaggca atcaagagaa aaccgtggat
    11041 ataatctgcg gtttgttata gcggagagag ggggattcga acccccggaa cccagagggt
    11101 tcaacggttt tcgagaccgc ccgattcaac cgctctcgca tctctccaat aagaatgagg
    11161 ctaagaggat tcgaacctcc gaccttcaga tccgcaatct gaagctctat ccagctgagc
    11221 tatagcctca cgaaagaccg gagagggagg gattcgaacc ctcggtaccc tttagaggta
    11281 caactcctta gcagggagcc cgattcggcc tctctcgcac ctctccttgg accagatggt
    11341 ttaaatcgga gcagggggga ttcgaacccc cggtaccttg cggtacagcg gttttcaaga
    11401 ccgtctcctt aaacccctcg gacaccgctc caatgtcagc agaccagtcg gcgagaatcg
    11461 acaatacgag aaagcatggt aattgtcaag tgctgaaagg gctaccatcg attgctctat
    11521 cagtccattt caaacctccg tccctgaaat gaatcacgaa ccaaaagttc ttcttctact
    11581 gcgcgggcca gtttgtgctt atcagtttgt ggggaaagat tgattgtctc ttcaggccgg
    11641 aaatctgctg acaaacgcat aatctcggtc gatggaggca acagctcaag acaatcagca
    11701 ataagaaccg gtaaacgaga tggatgcatc aatgtaatct cgcccgaatg gaaacttcgc
    11761 gcgaatgctg agccttttac caggtgtaaa tcatgaaact tcaatccctc tggctttatc
    11821 tgggcaacga atttaatagt ctcaatcata tctctgtgag attctcctgg tagtcctaag
    11881 attatatgta ccgttctatt aagctgagct tttttggcaa tatccatcgc aatgagaaat
    11941 tcatcgatac catgacctcg attaataaaa gcgagtgttt tctcgtgcgc tgactgaagg
    12001 ccaaattcga tccatacctc caaatcctgt tttctatagg atgctaagag ttctgccttc
    12061 tctatatcaa aacaatctgg ccgtgtagat acaataattc ccttcaaagg tgtcatagag
    12121 atatcagata acttcaagtc aacgctagct tgcttttgag tatcataata aatgcgcatg
    12181 aatgtcttta tggcatcgtt gtatatttta gctagcatag atacaggcgc gttagtacaa
    12241 gtatatgcct gaaagtagag gaaaaaggct tttgccttat agcgttgacg ggtaaaagcg
    12301 agagcgcggc ggatttgatc ttcgatagtt gatctatcat tgatagcttt ggtaacacta
    12361 gtgttgctat tggggacgga ggtttgtgat ttctggtatg ctgaaatatt tccatctgcg
    12421 gcgcaaaatc ggcaccctcc ttgcaatcga tctgcactgc gattggggca agtgaaacct
    12481 gcatcaaggc ctaatctcca tactttctca ccatagcgag cttttaagca ttgagagtgc
    12541 caattgattc ttactttttc tattgaatca agggatcgtt gcataatttt gcttgacctt
    12601 catctttgct agcaagagat ttgttactat gatgacacta tacatgaaga ctattcaagg
    12661 aagggaaaat gtacagacga tgcacttttc gcattggaat tagcatattt gccctaataa
    12721 taatcctttt atgctctgca tgtgttcatt ttacttcaag tgcacttatt tggacagata
    12781 tgcccgaact catcattgca gctcaacttt ttaatcgaga gaatgatcaa tataccatcg
    12841 atgtagaata taaagcgtat ccagcttctg agataaataa tgccactaaa cctccgtccc
    12901 taataattgc aaagtacatg ctttctaata ctgtattgaa aaaattcgcc tcattggatg
    12961 agctttttac caagtattat cttgatccaa acgatatata tccagcattg ctcaatgcag
    13021 gcaagttagg ttcaaggcat gtcttgttgc ctgtttcttt tgattgtgaa ttgatcatag
    13081 aaaacaagtc tgaaaccgct agggctggta cagccatgct cgattccgaa atgattcgcc
    13141 aggcgtctca agcttttact cggatagaaa atggcaagat tagcagccta ggattttctc
    13201 cacggtggga tctgagtttt gctgagaatt ggcttcttgc aggaaatgtc ggttttgctc
    13261 caaatcaaaa ctggaagcta acctctgtcc ccgccgtatc agctgatatg agcttgtggc
    13321 caattttatg gaataagcaa aatcttgaaa aatctattga agtcttgtta tcctttaatt
    13381 ctgatataag aatatctgac caagatgctt ttgcatttgt ttatttcaac aagcctggat
    13441 accagcttgt ccttgagggt aaagtgcttt attggcctat gaaggcaagt gaattcttca
    13501 agcttcctca ctcaacaaga gaacagcttc ggtatcgctt tccaacagta gaaaataagc
    13561 tcatattgac ctcagattcg agatacatgg gaattcctaa gggctcaaaa aatcgaaaag
    13621 cagccttaga atttgctaga tggctcttgg tgtcaacgaa ccaagaaaag atatggaagg
    13681 aaatggaatc ccagcagctt ttgcctgatt atgtaggtgc ctttgatggt ttcagcagca
    13741 taatgcaagt aaatgaaact atattcctgc gatattttcc tgattatgga caaaacccat
    13801 tattgtcggc tgctatacct gagcctgccc atattccaga ttattgggaa gacttctcga
    13861 gagatttttt gcatcattgg ctcgaaagcg tgatgtctaa tcctgattca aggagagcaa
    13921 tagatcaaga tttccgtatt gcttttgaga aatatatagg gacaatgcct gattggctaa
    13981 tctctaataa ttgatattat cttttgataa gctcagtcta gctatatatt ctggtaatta
    14041 tctcaaaaag gggtttgtac tcatcaatag tagagcagcg aactgcttgg gcgcgcaatt
    14101 cagctcctcc agggagacct cttgtataag cgcaaaagtg ttttcgaaat tctatgcatg
    14161 ccctcgtctc accgatatat ttagtagaca tttcgagatg gcgaagagcg gtttgagcta
    14221 taatatctgg tctcctatag atgaaagtgc tttctgggtt ggaggggctt tcaaggaaga
    14281 gggacggagg tttgttagaa tatgcctcgg catttctgcg atgggccaac tcatgaatat
    14341 ctgaaaatat gaaaggatta cctatagctc cccttgctat catgacacca ttgcagccag
    14401 tttcgcggat catagacatc gctgcgctag cgctcaggac gtcgcctgaa ccaaatacag
    14461 ggacagaggt ttgctcagat aaagctttga tcgcctccca gtccgctttg cctgcatagc
    14521 cttgggcacg tgtccgggca tgcagtgtta tagcacatac tcctgcttct tcagcaatag
    14581 ctgcggtttc aagataattg atagaattct ggtcccagcc agtacgcagc ttgatggaaa
    14641 tggggacgga ggtttgatca cgcatcgcgt gtacgatttc gccaatgcgc ttaggagttt
    14701 taagcaatgc cgctcccgct cccgctttta tgattttggg aaccgggcaa ccacaattga
    14761 ggtctattac aacgggtttg taagtagctg ctatttgtac ggcttttgct aaagttgtgg
    14821 gattagcgcc aaagagctgg atagcaaaat atcgctccac aggatcacgg gctagaagga
    14881 gttttgtttt ggggtgacct cgtataagtg cttcagcgct taccatttct gtatagcaaa
    14941 gtgcagcccc catttcaaaa cagatggctc taaaagctac atcagaataa ccagccactg
    15001 gtgcaagaaa aaatggcaca ttccatgagt cgagattcgt cgaaaagggg atcttttttt
    15061 tcaatcgcct ttcaaatcct tttgtctatg caatacaatt ttcaaggcgc tagcttaaag
    15121 aatcggcaag cgttttggta tgttgtttca gccacttctt ccacatcgat gttcaaaaaa
    15181 tctgcaagga attgcgctgt ggatggtagg tattcaggca tatttctctt atttctatat
    15241 tctgcaggta ccatgaatgg cgcttcgctc tcaagcaata tgcggtcaag cggaaggaat
    15301 gcaactgtct catgcagatt tcgcgcattc ctataagtaa gattacctgc aaatgaaaaa
    15361 tagatatcca actcaagatc gaggatatcc gctgcataag ccgcattttc cgagtagcag
    15421 tggagaactg cccctgcagg tggtagtcta tcctttagga tatccagcac atctttacca
    15481 gcttctcgat tatggataat gacaggaaga cctaagcgag cagcaatctc aagctgttcg
    15541 ataaaaagct ctatctggct gcgcctatca ccaaatttgt gataataatc gagtcctatt
    15601 tcgccaattg caattactct taaaagtttt gaagcttctt cgataaatcg ctgccagtcc
    15661 ttgcccggat tatttacttc tgatggagac acaccgacag catggtagac atattcagca
    15721 ggttttagat tctcatacac ctgtttgaag tccacgaggc tgttgcagat gctgactatg
    15781 cggcttacac cagctagttt ggctttctgg caaacaagaa gctgttcaat cgggtcatca
    15841 taaataagcc cgatgtgggc gtgagtatca aaatacctca tggtttttcc gatagaaaat
    15901 ggtgggcagc ccatgcgggc tgtctaaaat gtaacatgta cgctccctgc cgtcaacata
    15961 aatttatgct ttgtaatttg ctcctctaaa aagctatgaa tgagctagtc caagatttta
    16021 gaggtcgcaa taaattattc aacttcttct cctggtattt tttgcaatta gcttttgtca
    16081 actttatcgg atctattgct ttttatgata tgtttgcatc gaatatcaca acaagcggag
    16141 agcctacatc tcgaaatcat gccctagata tgtttcacgg acaagaggat tttcgataat
    16201 actctgtggc tggccattcg ctactatagt tcctttgttt attatatatg cacgcgatgt
    16261 aatagcaagc gtgtcacgaa cattatgatc agtcaagaga atacctattc tttttttaga
    16321 gagattatga ataagaagct ttatttcacg tatagcaata gggtctaccc ctgtaaatgg
    16381 ttcgtcgaga aggaggaatt tcggttctac tgctaaagct cttgctattt cagttcttct
    16441 acgttcacct ccagacagag tatatgctgg ctgttttgca acatgtagaa ccccaaattc
    16501 ctccataaga gcatgcgcct tttctcttct tgcagcggag gataacccac tacgagcttc
    16561 gagcacagca aggatgtttt cttctactgt catcctgcga aatatagatg attcctgtgg
    16621 gagatatgag attccaagta ttgctcgcct atacatgggg agattattaa tcatcattcc
    16681 attgaaaagt acagagccag catttggtct gagaaatcct gcgatcatat agaatactgt
    16741 ggttttgcca gctccgtttg ggcccagtaa accgattacc tcaccttcct ccatggaaaa
    16801 actgacatca gagaccgcga gtgtatgacc aaaggatttt cttaggttga ttacttcgag
    16861 ctttgcattg gtcattgaga tgtggcggtc tcctttgtgg tgatagtccc tgagacttcg
    16921 ccagagatct tgagtttaga ccagtcagcc gaggaagaaa tgacatcgcc tttaaggacg
    16981 ccatcttcag tcactgcaat agcaccgcca tgaagccata tttcctcagt atcgcgatta
    17041 tataatgcat attctgaact tatcgacata tcttccttgc gtaccttcac tgtgacagct
    17101 gcttcgaaga ttgattgttt ttggtcgaat cttagccact cagcctctat gatagtgttg
    17161 ttttttgtat cgttgacaaa taccttcttc tgagcaaggc caatctcggt atctcgattg
    17221 tagttgagct tttcagcgac tagcgagaat cctttttccg tgtcttgtac agtgacattg
    17281 ccaaggcata ctacattgac gtaatcttcg ccgctaagat caacgcgatc cgctgtaatg
    17341 atcatgcttc ctgtcgtaat atgcactgat cccataagaa tggtattctc tttgcctttt
    17401 gcgataactc cttggacagt attagcacta aagacaattg gctttttacc gctagaaggc
    17461 tgagtctgtt gagcttgcgc tactggctca gggctaggct gtagagaagg tggatttgtt
    17521 tgagcaaaga taatactaag tgatccgaaa atcatcatca agcctaaaat caatgcaata
    17581 atcttcagtt tcatactgtc attctacctc atcttgttga ttcactgaac catggacatt
    17641 ttcttgaata tgataatatt gttgcttaac atcaaaaaga aggccatttc cagccaatag
    17701 aagctgattc cctactttta tcagaacctc ggtatcatgg ggtgtctcaa agacatcgag
    17761 gcttctttta taactgagtt tggtggactc gaaagatgca tcgccttcag cactctgcaa
    17821 atgcacatag cctgagaact ccgcgtctcc gctaacttca tagaatacgg ctttttctgc
    17881 atcgccagaa gctgatacag agccatcgtc gccattatag gtaatgaaag aaagctcttc
    17941 aacctccaag cgctgatccg tttcatacca ggaagcgctc ttggcggaga attgcatgga
    18001 tagttttcct tctcgatatt cttttcttat gatatcgaca aattgagcat ctgggatatc
    18061 ttcttgagca tcaatcgaat tctcttcggc tacttgactg caggaagaca ataggataag
    18121 caaggtgcag aaaagacaaa gagaaaaaat ggattgagtt ggccattttt ctcttctata
    18181 agcgcccata tcctatgctc cttgttggat gggtaaaaca ttttttagtt tgtactataa
    18241 tccggagtgg ttcaaggctg ctctgatgaa ttctttgaat agaggatgag gagcggtagg
    18301 cctcgatttg aattctggat ggaattgaac gccgattgac catgggtgat ttggccattc
    18361 gcatgcttcg attagctctc catccactgt tgtagcgctt acacatagac catgagcttc
    18421 gagatcagta cgaatatcgc tattgacttc atagcggtga cgatgccgct cactgatcag
    18481 ctcgacacca taagcagctc gaatgttaga accagccttt gtgaccgcat cgtatgcgcc
    18541 gagcctcata gttccgccat agttcttcat ttttctttgt tcttcaagca agcacacaac
    18601 agggtgttga gttgatttgt cgaactcggt tgaatgagca tcttgccatc ttagtactgc
    18661 acgtgcatat tcgatgacca tgatttgcat acctagacaa atgccaaaat atggaatgtt
    18721 tcgttccctt gcatactgag cagcgcggat cataccttct attcctcgct cgccaaaacc
    18781 tcctggtatt aaaataccat ggatgtcctt gaatattgca tcgaaatctg ccgctgggtc
    18841 ttcgagacgc gacgaatcaa ttctttcgat atcgaggccg gtatggtgtg caatacctcc
    18901 atggatcaac gcttcccaga tcgatttata tgcatcttga agatccatat atttgccaac
    18961 tactgctatt ttgactttac ggttaggctt tgttattgcc tgaactacct tttgccatct
    19021 gcttagctcg gcgtggcggc tctcaacatt cattttacga agcaggaatg tatcgagctt
    19081 ctggttgtaa tagatgaggg gcacttcgta aattgttgct gcgtcgtaac cgctgacgac
    19141 tccttcttct tctacattgg tgaaaaggga gatcttgcgg cgaagctcat cactcagggg
    19201 ttttgtcgag cggaggatta gggcatcagg ctgtatcccc acttcctgca attccttgac
    19261 ggcatgttgt gttggttttg tcttgatctc gccgctggag actgcgggaa ccaaagtgac
    19321 atgaatggag attgcattct gcttgccaaa ttcgtgtatg agctgtctac aagcttcaag
    19381 gaacggcact gactcaatat cgcccaccgt gcctcctatc tcaacgattg tcatgtcgac
    19441 ttctggattg tttgcgacag ctagtatgcg ctctttgatt tcgtcagtta tatgtggaac
    19501 aacttgtact gtgcggccca gatagtcgcc ggttctctct ttttcaatga ccttcttgta
    19561 gacttggcct gttgtgattg agtttgccct gcttagcttt gcagtagtga atcttccata
    19621 gttacctagg tctaggtcgg tttcggcacc atcatcagtg acatagactt caccatgttg
    19681 ataagggctc attgtgccag catcgacatt gaggtaaggg tcgattttca ccatttgcac
    19741 attgaaaccg cgggcttcca tgagagcacc gatagaagac gccgcgatcc ctttcccaag
    19801 ggaagagcat acgcctcccg tcacgaataa tagttttctc atgggtcttc agtatcccaa
    19861 attgggggac ttgctgtcaa tcctccaagg aaaaaagtgg agataagaac cacagaataa
    19921 gtcaatacag attattttgt tgtactatga tgtatgctaa gaatattgga tattaaatcg
    19981 atgtatgcac agtgcttcta atgatataat aaaaacaaat ggagcaagga caattattgg
    20041 cgcgtggatt agctgcactc gactttgata tttccagtgg gattctcgat aaattgttgt
    20101 attatatcga tgagctagag cgatggaatc ctgtctattg cttcgtcaaa gcggaaggtc
    20161 aagatctaat tattaagcat gtcttggaca gccttggccc ttggcgaata ttggaggatc
    20221 ttttcattga gatggataag caattctccg ataataaaaa gctgcgtatc accgatattg
    20281 gaacaggagc aggattcccg ggtcttccac tagccatcgc atttcctcaa agatcttttc
    20341 ttttgattga aagactagag aaaagagctc gctttctaga aaatgtcatt tgtatactag
    20401 gactcgaaaa tgtagaaatt catcaaaaca ccgtagaaga ggaagacgaa ccattgggat
    20461 gtgttatatt cagggcattg aggccattca cagataaaaa attattccga accatttgga
    20521 aaaagatcca attgggtgga gctttgtgcg cttataaagg tcgcattatg catgcaaagc
    20581 tagaattgac ggggctcttg gatgatcctg tgcttagcag catggcagct catgccaaga
    20641 ttactccagt ctgggtacca ttcttggaag aagagcgctg tttggtagtg gcgagaaaaa
    20701 cgctgaacat ctgaagatac attaggatag atggatggga gatggatggg gacggaggtt
    20761 tttgcgacta tacatgatgc atcaatcctt ttcgaataca tgtcttaata catggtgaca
    20821 tgcagggacg gaggtttaaa ggttaattat tgaaaagatt ctagctagaa agattcgagt
    20881 accactagta ccatgagaag gacatttaag aagtcaagca agccttgaca ggcaaagagg
    20941 gcaaagagca gtattacctt tgataattta tcgaaaaatc ccaaaatttg gccttttcta
    21001 taatcacttt gttcgagcga atggggacgc cttcgttttc gagaagaaat cgcttcatcg
    21061 caattccccc ttgatatcct gcaagatatc catttgatgc tactatgcga tgacagggaa
    21121 ttagaagagg aagcggatta tgtgagagcg agcctgccac cgctcgaaaa gagagctgtc
    21181 tcccacaagc tttcgcaagt tccttataac tgcttataaa accgaaaggg atagttgcct
    21241 ccatcaagag caccttttgt ctaaattccg gtattttgag atgtgaaaga gaatccttga
    21301 tgatgttagt aggtggctgc actgggcggc ctgaaagcat gttcaaaatc aattgcaaaa
    21361 tcagttcgat atgttgaggc aggggaactt tttttctcca ttgctctaag agatagaaat
    21421 cactctttgc ttggcttatt tgagataccc cctctttagg aagatagata cggttaactc
    21481 ttggagaatc tatgtcttgc cagataagaa gaatatctcc aaaaggagat ggaatattat
    21541 agtagaatgc tggcaagtat tgtggcgatg gcaaaaaagt tgtccctggg ctttttatca
    21601 tatttggact attcattctg agaatgtctc ctaagattat tggtaaatat ttttctagcc
    21661 ctgattccta ggttgtcaac atttagaggc aatgctatcc aattgtgggt tgctttttct
    21721 gcaattcgtt tctcaagcac ttcattcaag ctctttgtag tgctggatgc gatgatgggt
    21781 gccttgggga cggaggtttc gaagattgcc ataatagtag gaccagagcc tgagatacag
    21841 atcatctttg caccttgttc gaggcatgca gtttttacta tgtcaaaatc aggaataaga
    21901 ggtcgacgat atggttcgtg gattcgatct gaacatgcac agcttaaaag attcagatca
    21961 gccttaagaa aagccagagc tacaagcgct gcatgagaaa taccgtgaac cacatcttgt
    22021 cttgaatagg aatctgggag agcgtttcgt gcgacgcttg tttcaagaat aaaatcagga
    22081 atgaaagcca tgaattgcca atgttcagga gctggattct gactgattac tatatccttt
    22141 tcggtgagcg ccgcagctgt aaaaccacca tagatagctg gagctgcatt gtcggcatgc
    22201 ccttctatat cggttgctgc tctaagaaga aaatactgat ttgcttcttc gtgtagaaag
    22261 cttgttagat tcgtcagatc aacctctgtc cctgtgcaat ctgttaggtt agcctctgtc
    22321 cccgtgccgc ccgtcagatc aacatcatca acctctgtcc ctgtaccagc tactgtgcca
    22381 gctagatctc gtagactctt gattgtcttt cctgaaagaa gaagtgcggc tgcagcacca
    22441 gccacagcaa gcgatgcgct tgacccaaga cctctagccg gaggaatacc cgtaatgatc
    22501 tcggcagaga ttttggggac ggaggtttgt aatagcatac agctatggcg aaaagagcgt
    22561 aggaaaagat tgtcctcgtt ggcccattct acagggcagc ctttgatctg agttctttcg
    22621 gctcgctcca aacgcacaat attagaaaga gacaatgcta ttccaaggca atcgaaacct
    22681 gggccaagat ttgcacaggt agcagggact tcgatctcta tcatgaaaac ctcacattgt
    22741 tgattttcta tagacttgcg gtttggcagt cttattagca aaatcagcca agactgattc
    22801 tagattcgac tcctcaacga tcatattatg atttatttta gcggtgacaa gatcagcaat
    22861 aggcttgggt acagagatac ccgtatagct tgaaagctca aaggctagcg aaagttcatt
    22921 atgagcaatg ggtgaatttt taagattgct ttgcaaagct gcaaggcagg atgctggaaa
    22981 cttaaagggg ctcgcagttg ctgcgatgac aatgggattg attgcctgag cttcgataat
    23041 tttcctatct ttttgatgat attgggaatt aacgaaaact tcgtgagcaa ccttgcaagc
    23101 aactgcagtg tgaggatcga caagcaagtt atggatttcc cacatatgac gaatgctctc
    23161 tagtgtctga aaatcatcag cccaccccgt ataaaagtcc gagagattac tcgcaacttg
    23221 ctcaggaatt tggaagaaac cttcattttc gaatcgatgc atcagtctca atactaattc
    23281 ggagttctcg ccaaaagcat gatagagcag tcgctcgaga ttgcttgaga ctaagatgtc
    23341 cattgaagga cttagcgttt tgttgaatgc tcttctttta tcatagatgc ccgttgcgaa
    23401 aaaatcataa aggattctat tagaattgga agcgcagact agtctccgaa tgggcagccc
    23461 catcgccttg gcatatcttg ctgcaagaat atcaccaaaa ttgccacttg gaacaactac
    23521 atcaaaagat tcgccacaat taatagcacc tgcagaatat aggtctcgcc atgcttttac
    23581 atagtatacg atttgaggca gaagcctccc tatgttgatg gaattggcgg agctgagccg
    23641 aattccaaaa aaaggagatc taggatcttt tgcgctttgg aaaatattct ttactgctcg
    23701 ttgagcatcg tcgaaggttc cctttagtcc aataacgcat cgatcttcaa gagggacgga
    23761 ggtcatctgc agcttctgga tttcacttgt cccttctgct ggatagatca ctgcgatgcg
    23821 gattccttgt tgtccaccga gccctgcaag agcagccgat ccagtatcgc ccgatgttgc
    23881 tgtaagtacg aggacaggtt catctattcc acatttctcg agacttttgc ttaaaagtcc
    23941 tccaagaatg ctcagtgcaa ggtctttgaa ggcgcatgtc ctaccatgaa aaagctcgag
    24001 caaaaaaagc tgcatcgatt cttttttgcc agccaagtca ttctcgcaat ttggcgatcc
    24061 tgcattcgat agcaatacta tttccggtgt atcgaacatc gattttttgc cagagctaga
    24121 gtaggctttt tcgataaggg gacggagctc ggtctcgctc caatcgaacc agggtgatag
    24181 gactagccat gcgagctcgg catagcttag ttttggctca agaattgctt cagggctcaa
    24241 tgtggggatg tttcgaggca caaaaagacc accatcttgc ggcatgccct gcacaatcgc
    24301 ttcggctatg ttgacatcta ctttagcatc cctagtgctc gtatatcgaa cgaccataca
    24361 tagagcatac aaaactcgca agaatgagaa aagcccgcgc aaggcttatt ctcttgcaac
    24421 gggcttatgt cagtgcatta tagtgatatt agtgcaacgc tcgatactca tatcttatta
    24481 aaatcgagtg gtgctgaaga ttctcgtttg ccgagttcag catctagcag atatagtccc
    24541 tgaggagaat tgccaaggag atctatcttt tggattatct cggtggcatt tttctcttcc
    24601 tcgacctgct cgtcaatata ccactgagcg agtatctgtg tcgcataatc tttctctgca
    24661 atagccatat ccatgatttc acgaatacta gcagttaagc ttttctcatg ggaaagcaca
    24721 tgctggaaga gaggcttaat ttcattttct ttgaattctg ggctatctat tttggtatag
    24781 gaaactacgg cattctgatc gtgtaggtat tctgcaaatt tcatagcgtg aaacatttct
    24841 tcatgatact gtgctgtgag ccatttcccc actcccgtat agcctagctc attcattttt
    24901 gcagccatag cgagatagag gtaagctgag tacatttctc tattgatttg taagttgatt
    24961 cgatcgatca ttttctgtga taacatatca ttcctcctaa tctattccga tagcatcaac
    25021 ggatttcatg gcaaggccat gtgctttaaa tataatgttt tttatataaa gcgcaaaata
    25081 gaagatagga tttatctaag atttgctatt tctgcatgtc taaagcaggt tataagatga
    25141 tcattgataa tgccaatggc ttgtagatgg gcatatactg tgatcgatcc gacaaatgaa
    25201 aaaccggctt ttttcatttc attgctgacc agatctgaaa gttctgtctg tgtggggatt
    25261 tcagaaatat cctgccaatg gttgacaatt gggcggccct cgacaaagtg ccagatccaa
    25321 gcatcaaagg aaccatattt tttcttgatg tcgcagaaag cgcgagcgtt tttgacagct
    25381 gcttcaagtt ttcgtctgtt tcgtataagc ccaggatctg aaagcaagct agttatatct
    25441 ttctcgcaaa agctcgcgac tatctctggc tcaaaattca caaatgcttt ttggtaagct
    25501 tcgcgctttc cgagaatagt tcgccaggac agacccgctt gctgggtttc tagaagcaag
    25561 aattcgaaat gagtttgatc atcatgaact gggctacccc attcttcatc gtgatatcgg
    25621 atatatactg ggtcatttcc acaccatggg cagcgaatca tagaagcaga atatcataag
    25681 caagaaagaa ggtggagatg ttgataatct ccttgcaagt gcaatatatt tgtttaacat
    25741 aagctacaga tttagtagtt tcaatgtaga ttggcagcta gagaggatca tatgagaaaa
    25801 tcgaaaattg ttaccatcgc ttttatgatg atcgttgcta tattgagccc tttgcttgca
    25861 cagaatgtga ctaaggaagt tcatgctgaa gccggtgaat tgcacgctct aggcggaata
    25921 ggttttggtt ggcgaggttt tggaataagt ggaggaatgg aatatatgct tcaaaagttg
    25981 aatattccga actttcctct ttcgctgggt gtgatgggga ttgccggcat ggattttgga
    26041 aaaggtttcg atctttctgc agctactatg gcaactcttc actggagcat gaaggcttat
    26101 aaagattttc ctaaattttt caagaatttt gattggtatc ttggtctagg tatgggcgtt
    26161 ggcatcgtct ctgcttttgg ttttgggatc tctactggag gcgggctttc ctattatgtc
    26221 aatgataaat ttgctataga tctacattct tactatatca attattttgt aggcggtggt
    26281 tccggtttta gcgaaagcat cgggattcgc tatactctga aataagttca agtttttgtc
    26341 ttttcgtttg tcattgttga taaggatatt tgcagtgcgc tcggatcctg attagagcta
    26401 tgcagcagag ctatgctgct cttttgtagg ctttcggctt tatattgacc tttgcatgac
    26461 aatcgctctt atgaaagctc tgtccccgag cacccgagca tatttcgtgg atacccaaaa
    26521 gatatttcca tcaaggaaat acatggaata tcggtgtgat actggtgata acttgcgatg
    26581 tctattctgg caagagcatg ccggctcgga attgaagcgt gatgattttc gatcctgaat
    26641 aacttgcgta gaaagtcata gttgcaggtg caagtccgaa agcgcgcgct tcgaatgaaa
    26701 aggacaatcc cgccgcccag tcatagatgc gaaagctctc atccgatgtg ctattccagc
    26761 gcgtatcgaa gaatggagcc agttcgaaac ctttcaggag caaggcttca gaaatatcaa
    26821 ggtataattc atgtggcgaa aatggaattt ccacatttgt gattaaattg gaggtcacta
    26881 cttttcttgc tgctgaactt ctaaatccat cgtatttgga ttccatgaac tcctggttat
    26941 tgagcgacac cttcgaaaaa gcacgaaact tggggctaac ccatttggaa tcaaatcctc
    27001 cctggagaga gccatacaaa cccgctgcat tttgtaatcc aacagagttt tccgcaaaac
    27061 ctcctagatt tgctgtgtac cacggctgga taggagggcc aaattttttg tattttctcg
    27121 gtatcgccgt atgaaaaaat gcgcatgtct gccaatttgc aattgaaaaa tccattttta
    27181 aaaaaatatc cccgcccgct tgaaatccaa tttttttctc ttcgtatact ttcatcaagc
    27241 tcgtacgccc tgcgatttcc caatattctg tgttgtgcga caatatcgaa gtaggagtat
    27301 agtcccccca gaggcgggtc gtaagccata cattaagact atctattgga ttcacgcgca
    27361 aggctgtttc aatggcccag acctttcccg gatctgggta aagggcgttg aaataagata
    27421 aatcggactt gaattttgaa tatgaaaaag aaagcgatgc tccaatgcga gcctctgtcc
    27481 ccgtctcccc aggcatcaat gtaaagggat caaaaagcct tagaatcggt atacctctca
    27541 gtgtaaacga ggtggaaggt tgttggccac tttggaggtc cctaatagtc ttgttaagat
    27601 tttcatgcat tttgctgcgc aattctaata atccggaagg gggagcattt tctagctctt
    27661 tcctagaaat gagatctagt tgctcaagag cattctgtgc acttatttcg ccttttttta
    27721 taattatgtc agggtcagta aattgcatat aggataggtt ttccacatca ttgcggatta
    27781 ccaaggcctt agatctttct attcctgcca tgcccgcacg agtttttcct atatctacgg
    27841 aacggttgat aactgtcact ggactcgaaa agttcatcgc tcggttatag aaggcagttg
    27901 aaatcacaag gcggttgctg aattggaggg caggttcgac aggcgcaata gtagtggctc
    27961 ctgcatcgat aaggatgaat tggtcgaatg cttggggctc gaaaacagct ggcattgcaa
    28021 atgcgcttgc catgacaaga tcaaatggac cttccgcgat ccatatctgt tgtcgagact
    28081 tgagatcctc agctgttatg ataattggaa tcgctgtctc agagacatcc agtctgccaa
    28141 ttatcttcct cagaatagcc ctgaacattt cggtattgat gaggccacca tttgtaggta
    28201 ttattggtgt aaagaaagac tcgagcggga gcgcctgaat taagaattca atgtcttgtg
    28261 gtgaatatcc agcagcatat agcatgccta caatcgctcc catggaactt gctacaatga
    28321 aatctgggta aataccggcc ttttccagtg tttttagtac gccaatatgc gcataagccc
    28381 gcgccgatcc tccacagagt acaattccaa gaggagggcg tggttcattg tccttgcttt
    28441 gatcgaactc gagggactct gctctttgct tgagccttgc aataaacgtt tcttctccta
    28501 agaacaccgg ttttgttata ttacgaagag tatctccttc cgggaaggca tattcgaaat
    28561 tattaaggga cgaaagctct aaaggaaaag gcgaatcttg ttgagcttga agattcgagg
    28621 ttgataagca tattaagaga agcgatagca gaataagggg catctgactg cgaattctat
    28681 agtcattagg aataggtttc atttcctgtt agtatatatt gatattagat tgacacggaa
    28741 gagtattttt gtgaattttt gaatattgca tgattatttc ggctgtcatt gtggcaaagc
    28801 gatttttgca tgcttctatt ggtgttttta aataaaagac aatcgaagcc ttggcaatgc
    28861 ctcgacaaag gcaatgtaga tttgctattc tttttctgtg gaaaacgatg gttcatctaa
    28921 aggggactct gattatttta tatgcataga ttttataggt gttgaagcaa aggggatagc
    28981 tctagtattt gcggacgagg tgcaaaatga tttctgaaat cctcgtcctt ttagctctca
    29041 ttctgataaa cggattcttc tctctctctg agacagccct cgtatcatca agaaaggcta
    29101 ggctcaaggc cgaagcagat aaaggtaaaa gtacttacaa gcttgccctt aatacacggg
    29161 aatctccaag ccgctatctt tctacaatac aggtagcaat aacacttatt ggaatactta
    29221 taggcacaat aagcggtacg actttttcgc agcttctagc ccgatggctc tcaagatggt
    29281 cttttattgg gagatatgcc gattctatag ctgtggctat tgtggtttta gcaataacct
    29341 ttctctctgt agttatcggc gaactcgttc caaaatcgat agcgctacgc aatcctgagt
    29401 atattgctgc tcttactata aagcccatgc atgctttgag cattgtcttt tatcctattg
    29461 taagatttct ctcttcggtt accgacagcc ttacgaagct tcttagattc tcgcataaag
    29521 ccgagactgc tatcactccg gaggaagtaa ggattctatt agaacaagga gaaaaatcag
    29581 gggtatttga ggctgctgag cgcgaaatga tggaagaggt gttgagtctg gatgataggc
    29641 gagtcacaat gttcatgaca cctagaaccg aagtagtaac gctgaatgta aaagattctc
    29701 gagactctca catcagggct attatcaaaa attccgaata tggatatttg cctgtagtcg
    29761 atggagactt ggatcacaca ataggtatgg tatcggttaa gcgagcgctt acgtgtatgg
    29821 cagaaggttg ctttttatct ttagaaacat gcatggagcc agcggtgatg ataccagagt
    29881 cattatcggg tttgcaagca cttgctattc tccgcgatgc aaagaagtcg gctggtctca
    29941 tagtcgatga atttgggggg atttcaggca ttgtaactat agccgatttg ctagaagcta
    30001 ttgcaaatct ttcggaaata gagacagaag aggctcctca gattttaaag cgaagcgatg
    30061 gctcctatct tgtggatggt tcgatgccaa tcgatgaatt tgccgagaat ctaggtatca
    30121 atctcgatat tatttcaagt gaggattacg ataccgttgc tggttttgtt ctccattgtg
    30181 ggggctcaat tcctaaagct ggcgatataa ttgaatggcc tcctttaaga attgaaattg
    30241 tggacatgga tggaaaacgg atcgataaag tattagtaca gaaatccgat taggctttta
    30301 tttaggtttt taacatggat agaattggct ctagctttta aagtccgaag gtctagaaaa
    30361 tccattttaa cgcctttttt tgaaaatcaa gtgtttttga agcatgaaat tgtaaaaaat
    30421 agatacaacg acatctacaa caagccttgt tagcttatag tctatcttgc tgagagatgt
    30481 aagggcaaaa gttcctgcgg attttagggc gatactgcct aagaccactg ggaaaaacat
    30541 gccaagctgc tgaagaacgg aaaattcata ttgtgcatca tttgaataaa agctccattt
    30601 tctattcacg ctgaaattta caaccgcgcc tacaatacct ccagcggcga tcgagatagt
    30661 ataatggata tgaaaggctt ctgtaagaaa aatcattgta cagtaatcaa ttacgccacc
    30721 aacaaaagcg gatatttgtg cttttgagaa agtaagagct gaacgcagcc aagacaatgt
    30781 tttttgttta ttctttgtta ttttccgcaa tttttgattc accctttctg ttttcttctt
    30841 catcacgctc attggcaact ttggctagtt ttttgaattc agaaaaattt gaaatcgcta
    30901 ggatgatatc tgctattgca gcgaggtaga cgatacttcc attgaaaaga acttcaatta
    30961 caagaaaaag agaaataaga atcctggttt cggtcgggct caatttgcct gcgtcgatgg
    31021 aatagctacc accgatctta tagcgaagca aggcgatcat tatttctaga ccataaagta
    31081 cgacaaaggc atatcccaaa attttttgga agctatttgc gtagatagca aatccaagac
    31141 cgataagtac gatacccaac caatctacca caagatcgag cataaatccg taccattttc
    31201 ttggcttttt tctatagtaa gctactcgtc catcgagtga atctccgaac caggaaatga
    31261 caaagccggc aacgccaata agaagaaagc gagtgtcaaa tagttctgca agaatgaagc
    31321 ttaaaaagac aatcacattt cctaaaaacc ctattgctgt gagcatatta gagctcatcc
    31381 aggaaggtat atattgaact aaaaaggcaa gggctttttg ttcctgtttt ttaagtagat
    31441 tggttcttat acgatcatgg gaaatgattg gtaatgtatg atctattgaa tttttctttt
    31501 gtttcattcc tatcttggca ttacctttat ccattttttg cctcaacatc tctctttact
    31561 tcaattgaag aatctttaac aattataacc gaatggattt ttgtctggca atgggctcta
    31621 aaaatacata tattttagat atgtgtcaat aaaagagttt gatattattt gggcatgagg
    31681 ctatgaacaa gaaaaggctt ttatatatcg ctatctatgc gatcgggatg gcatatatcg
    31741 aggcatcaat tgttgtatat ctgagaaaac tttatggaat ttctgatttg atgataagtg
    31801 tgccaagctt cgatcctcaa gtatcgatca tcgaactggg gcgagaattg gctacgctca
    31861 ttatgctttt tgctattggc tttgcctctg gcaagagatt tcaatcacag attggttttt
    31921 ccctatatgc atttggtctc tgggatattt ggtattatat atggcttgtc gtttttatcg
    31981 gattccctca ttcattcttc gattgggacc tgctctttct tattccgctc ccatggtggg
    32041 ggcctgtgat tacgccggtt atgatctcag ttctcatgat aatcagcgga gcgaggcttg
    32101 cattacttga ggagagggga ttacttgtga aacctagatc agttgactgg ataatgctct
    32161 ccattggggc ttgcatcatg ttgtatacct tcatgagcaa tgcaattggg agtttgccaa
    32221 cgagtgtgga taacttgagc gatcttagac cgagctcatt cttatggtca atttttcttg
    32281 taggatatgt aattgctacc tatgcaacct ggagaattct atataaaagt ctggatgaac
    32341 cagcgatgca caggagcgtc aggaagtagt tcagggtgaa aggtcgctgc gactatcgag
    32401 ccttggcgaa tcattacggg ctcatcctga tacattgcaa ggacttcgac ttcttttcca
    32461 gaagcgagta tgcgtggggc tcgtatgaat attccttcta ttttgcctat gctgcttatg
    32521 atggtttgta ctggtgcatg aaagctgtct acttgacgcc cataagcatt gcgcttgacc
    32581 ctaatatcaa ggagaccgag gctcatttga ttatttccta cgatatcctt tgcgaggaga
    32641 attaggccag cacaagttgc aagtattggc atcccattta cgattcggcg ctttagttcg
    32701 tcgaacatgc cgaagcgttc gaggagcatg ctcatgaccg tgctttcacc tccagggatg
    32761 atgagtgcgg tcaaggactc caggtcggaa aagcgtcgaa cctctacaat atcgcagcgg
    32821 gggaatccaa cacgctcaag ggctttgccg tgagcttcat agtctccctg aagcgcgaga
    32881 agtcctatgc gatggctgct caccaacccc tctctgagat tttttgattc tcagcaaggt
    32941 catcgacgcc gatgccgacc atcggttcgc cgaggtcctc cgagatctct gcaaggattt
    33001 ttggatcttt gtaatggaca actgctgata cgatggcttt tgctctgcgt tcaggattgc
    33061 ttgatttgaa gatacccgag ccgacaaaga cggactctgc accaagctgc atcatcaatg
    33121 ctgcatccgc gggagtagca acgccaccag ctgaaaaatt gggaacggga agtttgcctg
    33181 tctttgctac ttcccatacg agctcgaatg gtgcgcctag ctcttttgct tcagtcatga
    33241 gttcttcctc tggaaggcca atcagacgag ctattccatc ccgaactgtg cgcatatgtc
    33301 gcacggcttc aataatatcg cctgtacccg cctctccttt tgtgcgaatc atggccgccc
    33361 cttcgccaat ccgtcgcagg gcttctccta gatcacggca gccacataca aacggcacct
    33421 tgaaagaatg cttatataca tgaaagcgat catcggctgg agtaagaact tcggattcat
    33481 cgataaaatc gacaccaaga gcctcgagta tctgggcttc tacaaaatga cctatgcgac
    33541 attttgccat gaccggaatt gagacagcat cttgaatttc ttttatgatt ttcggatctg
    33601 acatgcgagc aacacctccc tggactctga tgtcagctgg gacgcgctcg agagccatca
    33661 ccgcacaagc tcctgccttt tcggcaattt ttgcttgggc agcattggtt acatccataa
    33721 tgacgccgcc tctgagcatt tcggcaagtc ctacttttat atgccattgt tcgctctgtc
    33781 ggtcggacca taatttttgt tccataatat tatctcctca ctgttagcgg tgtggaagga
    33841 atctgcttgg ttgcaagtct aaagagtagc gaaaaaatac ttattacgca agggttggag
    33901 aaaaggttaa gggtgtcgag attcaagcca ttgctcttta agcaaacttt aatgcgatct
    33961 tgcctattaa gatgttttta gcgcgattct ttcaaatgtc taagcagagt cagtattcga
    34021 tatcgaaaat tttccctgga ttcataatga gatttgggtc ccaagcgcgt ttgattgcct
    34081 ttattaggtt cagttcgaca ggactcatga atcgtttcat gaaatccttg cgctttagcc
    34141 cgatgccatg ctcgcctgat attttaccgc caagtgcggt gatcgattcg tagagtgcaa
    34201 ggagagcctg ttgctctatg cgttgccatt gttccattgg catctgtgga tccttgacga
    34261 gagtagcatg gagattacca tcgcctgcat gaccataaca tggtatttca atgccaaact
    34321 gtgaagagat gcgctcaagt tctggcataa cggcggatat ggcggctggt ggcactacta
    34381 tgtcctcgag gctctgaacc gggcaataga cttttaatgc ttcggctatg ttcctgcgaa
    34441 tagcccagag gcgctcttgg gttgtgcggt tgtcagcgac atatacttcg atagcgcctt
    34501 gttccatgca taagtctcca actgcctcag cttcgctttc gactatagac tgatctcgtc
    34561 catccatttc gatcagcagc atcgcaccgc attgagcata gggcagattt tcgtttagat
    34621 aggcgcagga tgtctccacg ctccggcgat ccatgaattc gatggatgtc ggaattagac
    34681 cctttgagag aatcacgggg acggaggata tggcttcctg agggcttcga tagagtacaa
    34741 gcagatcgga ttttgcggcc ggaagaccaa tcaaccttat tatggcagca gtcacgatgc
    34801 caagggtacc ttcggagccg acaatgaggt gcactaaatc ataacccgtg acatcttttg
    34861 caagcttgcc tcctagccag actacctcgc ctgttggtgt aacgaattct aggccaagga
    34921 tatatcgaga tgtcacgcca tacttgatgg cttttcctcc tcctgcattt tcagctatgt
    34981 ttccaccgag catacaagtc tccaagctca tgggatatcc tgcgtagaaa agcccttttt
    35041 ctttgactag gttattgatt tcgtttgtca cgataccggt ttcgacggtc attgtaaggt
    35101 tatcgtaatc gatttcgcgc acttggttca tcttttcgag cgacaatact attccgccga
    35161 atatgggaat ggcacctcca gaaaggccgc tgcctgcgcc gcgcggcgtg atggggacgg
    35221 aggttcgatt ggcaagcttg acgatttcgg ctacggcgga agtagacctt ggagtcacca
    35281 ccacttctgg catatggcca tattcttctt tggaagtttc gtcgtgcgaa aagatttcga
    35341 gctgagctgg gtcgaggagg acatttcgtt caccagcaat attgatgagt tcgctgatta
    35401 tgtctttgct gacaggatta tatttcatgc tgagcctctg tgcttaagat ttctgagcgg
    35461 ggaaggtctg gctcttgcaa ttttgtatct ttggtttgac atggagaaag agaagaagag
    35521 ggggatgaag aaccatgccc aaggctagat acagacccag gcctaagcac agacccagga
    35581 ctaaatccag gatcaagcct aggctcaggt ttagactcag atccagacga agagctatag
    35641 attgatttca attgcgccgt gagccgcggc aatatctcga agagatcccc tattatggct
    35701 agatctgcga ctgcatgtat tggcgcatct gggtctgtgt tgatcgaaat tattttctta
    35761 gctgttcgta tacccgccaa atgctgaacg gctcccgaaa tacctgcgca tatatagatt
    35821 tccggcgaga tggtcttgcc gctaaggcct acctgatgtg ggtaggatat ccatccacga
    35881 tctacagatt cacggcttgc gcccactgct ccgccgagag acgaagccag ctcgcggatg
    35941 agcttgaaat tatcggcttt ctttaagcct cttccacctg acacaacgat ttgtgcactt
    36001 tccaggtttt cgaaatcctt aacattgctc tctagagcta aaacttggac agacaaagcc
    36061 aaagaatccg aaataatttt gcctaaagat gaatcatcga ttacatgtat tatgctgccc
    36121 ctgcggccaa agtccggtgg gagcggctgc atggagtgag gtcgtacggt ggccatctgc
    36181 ggcctgtggt gggcggtttt tatggtggcc atgatattcc ctccaatggc gggacgtgtc
    36241 tggagaagaa gtccgctgcc ttcttcgatg gagagctcgg tgcagtccgc agtgagccca
    36301 gtgccgagtt ttgcagcaag atatggcatt agggttcttc cagttgtagt ggcagctcct
    36361 agaatgactt cgggttttct ggcgcaagcg agctcaaaaa gcgcttctgc ccataactca
    36421 cagatgaagt cttccaaact tgggctttcg actagaatga cgctatcagc gccatgagca
    36481 atcagcatcg cagcttcatc tttagatgag gggccaagca caatagccac cagggttgcc
    36541 ccgcggctat ctgctagatg tctggctctg gccagcaatt ctaaagacac tagatcgatt
    36601 ccacccttgc ggcgctcggt aataatccaa aattctggct tatcgtccac aataggtcct
    36661 gatgagagct gttcagaaag agaaatcatt gactcctgca ttgcaggctg gcttttggct
    36721 aagctgactt tagtctctga tttttcgata ttcgctacct tatgtattcc cgaacatcct
    36781 ccgtccccca ttattggacc aatcgtagaa agaatgcggt ctattgcttt gtctattgct
    36841 gcttcgtcga gcgcaagaat tcgctctccg ccgcgcgcta cctttgggta aaaaatcttt
    36901 actacacggg taggagagcc tgccaaaccc accgaagccg gatcgagatt tatctccttt
    36961 gctccccaat gagaaatttc tgcacttctg gagaattttt ttccgcgcag agtcggcaag
    37021 cgtggtgagg caatttcctt gactacggtt attaggcagg gggactggac tcttaccttt
    37081 tgatagcctt cttcggtaag gcgacggacg agaaatgtaa gcgagctagt tctattgtgg
    37141 tctgattcag caattcgtga atctttagtt ctcgattcta tttccagcga actcacataa
    37201 gttagcgcgg gtattcccaa ccaagatgct accgcaggtc ctacctgccc agtatcgcca
    37261 tcggtagcgc gctctcctgc aagaatcaaa tcaaaagtgc cgatatgttt tattgtctca
    37321 gcaagaaccc tagaggtagc ccaagtgtcc gatcctgcaa aagccttgtc cgtaagcaga
    37381 attgctttat cacaacccat tgcaagcgat tcccgcagcg cacgttcggc atctagcggg
    37441 cccatggaaa ttaccgttat atgggctccg aaatgctcct ttagcataag cgcttcttca
    37501 attgcataca gatcgagagg attgataatg ctttctaacc ctgatcgtac catggttcct
    37561 gtctcggggt ccattttgac tttatctgtt tctggtactt gtttgataag aactattatg
    37621 tgcattgaaa aactccggaa tgcaagtatg acacaggagt atgacatagt agtagataga
    37681 tccgcaagat tatagggtat gttagtggga tttggagtgg attctcgacc aaaaggataa
    37741 gatggctgcc acagctgatt agataccgca agctattggc agccatcttg ctattctatt
    37801 attaggcttt tatatcctga gccgtagatg aagtgtctgt ctgccctgct tgcgaggacg
    37861 acagggcctg agcttcttgt gtctctttta ctagtttttt agcctcgttt gttttctttt
    37921 tctgtgccga cagctctcgc atgcgtttcc atatcgaagg aaagagtaag agcataccaa
    37981 tcaggatccc tgcggcgaag gtcaatacca acacaatcga cattgaagct tgggaagacc
    38041 atccgaaaaa cgaaacggta ataggtacgc tattctgtac tgcaaagatg acggctagaa
    38101 tagcgacagc aatcagaaga acaatgtaaa agactctcat tttgcctcct tatatggagt
    38161 ttcttaagaa ggtctttgct ctttattatc aatggtatca gatttccaat gctcaggggc
    38221 acgccacagg ctcgataaca ggagttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38341 nnnnnnatgg tatcagattt ccaatgctca ggggcacgcc acaggctcga taacaggagt
    38401 tgcctaacga agatgagttc ttttggtagg cgttcataaa gctttacaaa cagctcttca
    38461 tgagaaagga tttcgttcgc ccaaagctcg cgatccaccg acataagttc aataaagcgt
    38521 tcctttgaaa aatcgaggcc ttcccagtcc aaatcctcat agcgtggcat ccatccaatg
    38581 ggactttcga tagcattggc tttgtggttg gcgcgatcga caatccactt aaggacacgt
    38641 atattctccc cgaaacctgg ccatataaaa ttgccatcag catccttgcg gaaccaattt
    38701 acgccgaata ttaggggtgg attagggtta gaaaggccga catgcagcca ataatcgaag
    38761 tatgaggcca tattgtagcc gcaaaatgga agcattgcaa aaggatctcg ccgtacaagc
    38821 ccaattgcat ttgtagaggc tgctgtggtt tctgaaccaa gagtagctgc tagatatact
    38881 ccataaaccc aattgaatga ctgatagact agaggataga ccgtgcttct gcgtccaccg
    38941 aatatgaatg cctttattgg tacaccttcg tgactatccc aagccggatc gatagaaggg
    39001 cattgctgaa tcggagctgt aaatctggcg ttcggatggg cagctggttt tccagaagca
    39061 ggatcccatg gctttccctg ccaatctatt aagcctgcag gtggattctt ggtcatgcct
    39121 tcccaccata tatcgccatc tggagtgaga gcacaattgg taaaaattgt gttggttcta
    39181 atagtagcca ttgcatttgg attggatttt tcggaagtcc caggggcaac gccaaagaaa
    39241 cctgattctg gattgatggc tcgcatttgc ccatctttgc ccggacgaac ccaagcgata
    39301 tcatcgccta cagtcgtaac cttccaacct tcgaaaggtt cgggcggaat gagcatagca
    39361 aaatttgttt tgccgcaagc actcggaaag gcagcagcca cataggtttt ttcatgagat
    39421 ggcgattcaa gaccaagtat tagcatgtgt tcagccagcc aaccctgatc tcgcgcaatg
    39481 accgaagcta ttcgcagcgc gaagcatttc ttaccgagaa gagcattgcc accatagcca
    39541 gagccatagg accaaatctc ccgggtttct ggaaagtgga caatgtactt tgtctgtcta
    39601 ttgcagggcc attgaacatc tttttgccca ggttcgagag gcatgccaac tgaatgcata
    39661 cagggcacga attcgccatt ttctcccagg gcttcgataa ctttcctgcc catccttgtc
    39721 attatgcgca tattggcaac aacataagca gaatcggtta tttctattcc gatgtatgag
    39781 aagggagaac ccaccggtcc cattgagaag ggaatgacat acatcgttcg acctcgcata
    39841 cagcctgcga agatcttatt gagaataccc ctcatttcgt ttggctctac ccaattgttg
    39901 gttggtccgg catctgattt gtctttgctg cagatgaaag tgcggtcttc tactcgagcg
    39961 acatcgctag gatccgacct tgctagaaaa gaatttgggc gtttttcagg attaagggga
    40021 ataaaggttc ccgatgcgac tagagagcgg caaattgtct catattcttc atcagaacca
    40081 tcgcaccaat acaccaggtc gggtttgcac aattcttcca tagaagaaac ccacgaaaga
    40141 agcttttcgt tttttacata ggcaggactc atcttaagcg ttttcatgaa attactccga
    40201 agattaaaga tataaatgtt attataggct ttttttttat tttgtgtatg gggaag
//
LOCUS       DAOSOB010000011        36498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1191_length_36498_cov_77.454683, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000011 DAOSOB010000000
VERSION     DAOSOB010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 36498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36498
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1191_length_36498_cov_77.454683"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLS88_02245"
     CDS             complement(<1..341)
                     /locus_tag="PLS88_02245"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HOU06203.1"
                     /translation="MKDQPIDKGKKQWVYPDLELPPPGDFPLKGHESLIVLNMNDSDA
                     SIIITLYFTDKEPVSLPALTVKARRVRCLRLDKEEEIGIQLPRETQYALRLNADVPVV
                     AQYGRLDTRQQ"
     gene            complement(433..1503)
                     /locus_tag="PLS88_02250"
     CDS             complement(433..1503)
                     /locus_tag="PLS88_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HOU06204.1"
                     /translation="MRKNKPIIMKLDPYMFSEKEREILASPSFCVSLFQYRTGVEALR
                     IKIGRGEFIWLPYLGQQLWDWSIDGKSLKFEGFIEEPSYGKNFLQNYGGFLIHCGMTA
                     MGNPGPEDTHLHHGELPVAKFDEAWIEMEPENGSESPVSLCGHIHWKVPFVAEYHCSP
                     SLNISADGLQASMEVLLNNPASKTMEYMYLAHINFAFSGAEKILSTVPFDSSHVTIRA
                     DGEEKTAALFADPSLIKQIDKKAKYEPELVAILNHRDIASEAGSILLHEDGTARWVRQ
                     QKPELDHHVIWITHNEDRGACGFHLPSTAGPTGYSNEKNLGNLKHLPAGCNATLRYDF
                     GFADRLEDVPIICTKSRLSNVG"
     gene            complement(1500..3605)
                     /locus_tag="PLS88_02255"
     CDS             complement(1500..3605)
                     /locus_tag="PLS88_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOU06205.1"
                     /translation="MINLFPEPKKLQEQDGYTKAFHALAIDFGNLSEAEKSDLLEIAQ
                     MRFWNCKDVKIFADTSNEESITINSIQSLSGIEASNRDLFIKQGYFLKIEKNRIVLRY
                     QEKSGFIYAITTIKQLLIKKDEMYLLPLCEITDWPSIEHRAVAPTFSWYAGYGRLGFD
                     MQLWCYEEWVKYLNSCIDCKINQMNMVMYGYWPFDFEEYPETVFKDIPVKIWNKENSQ
                     WLTIHYSHPNIEQNFLADFITLAHRFDFKIFAYVGLNSYNGAYSIKHPEKRMIKPAGS
                     NFMNDFDSLCLSNEDNITYILASMRKIAQLGFDGFTLEESEEGFWFCNCEKCTERWGK
                     TSASPGEAKHKANMWLLNLIYKEIRKINPQAVIGIRAFRQPPLEKDPVFLEECVRSMP
                     DDVVLFWAPALYVPPTEFKKWIKAFGKERIWGRDSEANSITSTMGRLFRMFESNIIRY
                     EDESNVQVIERDIEQHITSAEEGVSGINGFMFEWYGLFMFQWAHGNYGWGSRMDKETF
                     FRRSCEASFGKELGQRVLLVLKSILTIHESQMPLYTTPFPFQKNKITEADIPLIMQAK
                     LRHPGLLEEIHAIQRELEKDARLTEYIPHFARIENAERRNGVIYDMVLASLEYEKATT
                     AEEKERLLDEILYYNEKDFDLVKDMFFDINPVSESGVKSCMYPYHEIKRIIHNIQHPE
                     NPDNEIICSGIEALGWLWL"
     gene            complement(3677..4564)
                     /locus_tag="PLS88_02260"
     CDS             complement(3677..4564)
                     /locus_tag="PLS88_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU06206.1"
                     /translation="MKSFHVLFGIDMETDVGSFTPFYEGARNGTPLLLDLFAKKGIKG
                     TFFFTGDCARRNPDIAKTVANSGNEVGCHSLYHETIGDELFPIPGVYPLLSHEVKPRL
                     ELCRDWVQEAAGVKTTSFRCPRLWGSTAVVNALEELGFTCDVSYPMYFYRQQFSPYHP
                     DRTDWTKKGDSSVLEVPNFADMTMQSKDPGLERDRDQWPLFRTEGAEYILKKSASFID
                     ICNKKGIPAVLAYYIHPWEFWPMKTSYHFGEATVIPDEFITKNCGPIALCELEKQIDG
                     LARMGAEFVTARDLAKLFA"
     gene            complement(4586..5758)
                     /locus_tag="PLS88_02265"
     CDS             complement(4586..5758)
                     /locus_tag="PLS88_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOU06207.1"
                     /translation="MHGNIHIPDYEYKERVSKAAALLKRDGLDALIVNGTEADYANTR
                     YFSGFWPLFERCGVAIGAAGDAALMVGPESRAFGGDRNKLNKLYVLKDYRESADPAYP
                     ELKADTFKDVFKGLGINGKKLRIGVASYLDTSVVIMEGIRKAFPEAEIVRADYLMVEL
                     RSIKSENEISCLKEGYRLAELASQQVIKEIKPGMTELQMVGVAQRAIYENGAEYEGLP
                     MYVFSEASTRHAISRSSYRRFEKGDIVQLNLSAKIDGYSASIGYPIVLGKLSGKRRDI
                     VMFALEAHRWTEAQLKEGVMAADVAKDFYQFYVSNGYANNFVYGPLHGTGLIEVEAPW
                     VETTSNYAFKPNMTFQIDTFISTDTFGVRWEKGIAIKENGVEVLSPPIGTLYELEF"
     gene            complement(5761..6777)
                     /locus_tag="PLS88_02270"
     CDS             complement(5761..6777)
                     /locus_tag="PLS88_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06208.1"
                     /translation="MSTFEEKSKRYGKSWRSFIRDWIMLFLLIALVVIFSITLSTFRT
                     SDNLMNILRQTAFTAIIAMGEFFVILIGQMDMSISSTIGMVSIFFAGFVVKMGMPIWI
                     AIILVLLMSALAGLINGILVVYGKMPSFIATLVTMNIIKGINYLYSRGLPISGLPDGF
                     NFLGSGYIGIVPFPVILMFIVAIILFIITAHTAVGRSFYAVGGNLEASKLSGINVVFV
                     SILAFVFSGVLSGVGALGLTAKTLSGNVSLGENLLFDVMTIVVLGGTSLTGGRGRIFG
                     VVIGALFLQVISNAMVLMGINTYWQWVVKGIILIIVVLIDSNTKREVVTTSQKARIAS
                     VEEK"
     gene            complement(6774..8324)
                     /locus_tag="PLS88_02275"
     CDS             complement(6774..8324)
                     /locus_tag="PLS88_02275"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU06209.1"
                     /translation="MTNRFQSDTDDTPKLNKTVPLIELRNCTMEFPGVRALDNVNFTL
                     MPGECHGLVGENGAGKSTLSKCITGEYRMTKGELFVEGQKIKLSSYSIKESQNLGIQI
                     VHQEFQLMSEMTGLENIFIGHYSRKGGLIDWKALNNKAKELMDFLQCDVNLNIPVHYL
                     RTAEKQIIQLAKAVQSKSKVLILDELTSVLQEKDIQNIFRIIDILKKRGMGIIYISHR
                     LNEIFECCDSYTVLCDGRHINSGNVSDIDQQKLIKMIIGRELTQVYPSINADFGDIML
                     EVKGLTAPKAFKNIDFYVRSGEVIGLAGLVGAGKTELIQAIFGVHPVISGKIFVKGKE
                     VKIKNPWHAIQLGLGYVPDDRRSLGLILKFDIKENTILPSMNKFKKMRFFQDHKAEAK
                     AAFDINEKLGLNYYSLFQEVETLSGGNQQKIVIAKWMLQNSQIFLLDEPTRGIDVGAK
                     FEIYKLIKDLTKQGKSVIIVSPELEELIGLCNRIYIIYEGMIMDTVEGERKRQEVIIH
                     SLLGVNGK"
     gene            complement(8476..9480)
                     /locus_tag="PLS88_02280"
     CDS             complement(8476..9480)
                     /locus_tag="PLS88_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU06210.1"
                     /translation="MKKIGSIMLIIFLLASTAGWAQKTPAQTKDNKTLIGVSIMELTA
                     YTWFLGVIDGCKQWASEHPEANFTFQFEDSHSDVQTMLNNIENLLAAGAKGIILFPAD
                     ASSAIPTMKQAVAKGIPFVIGDYKQQPAKPSDAVWATFVGHDMRALGVVAGKIAVDYL
                     KTLNKPNPVCLFVSRPTSGQVSKDRFEGFRDTVLASFPKAKVIEEGDVGAGNRASSQD
                     LVENVLKREPVIDVISGHNDAEVVGAYNAAVSQKRTNIKFIGIAGDKDVLSFISKGNP
                     AWIGEVLQDPVVLGYQATDAMYRALKGEKLPETYNLPLPEAITPANIKNYNWQSWSWL
                     "
     gene            9817..10845
                     /locus_tag="PLS88_02285"
     CDS             9817..10845
                     /locus_tag="PLS88_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU06211.1"
                     /translation="MTKLKDIADETGFSIKTVSRAIHDHPDIKKETKEIIMEVVRKRG
                     FTPNWAAQSLRNQKTNTIGYIIPNITNGFFSEIGIAIDAFFREKGYSTLICFTSDSRE
                     NEIESIRSLLAKNIDGIILAPIGNRGEFIDEIPNLADTPLVLIDNRLSGPPRNYVLQD
                     NEGNSRRLTAHIISHGHANIGFVTGPLEESSSRERFLGFCNALEDAGAIYDESLVRTV
                     DWEIHSGYEATKDLFDHKGNTRPTAIIYGNTQLLLGGYKALREKDLLIPQDVAVASFE
                     HPDIIDALTPCPTTLEKVEKRIGIAAAEMLLSLIETKEEIPPQEILIPSQLIIGESCG
                     CFARSQNP"
     gene            complement(10966..11592)
                     /locus_tag="PLS88_02290"
     CDS             complement(10966..11592)
                     /locus_tag="PLS88_02290"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU06212.1"
                     /translation="MTDIANNVEELYSVLRRRILDEYYFAGQKLSENALAKEFECSRT
                     PIREVFKRLEQDGLIVVKPKSGSYVRIYSGKNYLDLIEVRAYLEGLAFRLSVERGADT
                     SEMEALNKTMDDIIATAPIDMMTYAEVHYRFHREFIRMSGNELLENFFERLNLKSSHL
                     FLQTMNPEIARKTQEEHWRIINLVKARDPKGEKFVIQHLWKKRDYLGY"
     gene            complement(11616..12839)
                     /locus_tag="PLS88_02295"
     CDS             complement(11616..12839)
                     /locus_tag="PLS88_02295"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOU06213.1"
                     /translation="MTVSHIHELRNFYYRELTDDILPFWLSHGIDNEKGGLLDFLDRQ
                     GQPLSTDKGGWAQGRATWIFSALYNDLEPRDTWLSAARSCADFTRDKVLAGPEGRAYF
                     ELDRNGNPLILRRYLFSEAFAIIGLAEYSKASGDSSYLRKAIDTLAVYDRFRGKLEPK
                     INPALRPMRGHSDTMIMINVFQVLRDADPEHASIYSSRIQDRIEELFRYFVKPEKKVL
                     LETVDPDGEMTEGCEGRTINPGHIIETSWFLMSEAHINGDKVLLNKAIDLLSWSLERG
                     WDTEFGGLFSFLDAEGKQPAQIEWDMKYWWPHCEALIATLMAYVSTKDRRWERWFETI
                     HEYTFSHFPDPVYGEWFGYLHRDGSIANTVKGNHYKVCFHIPRCMLKVISLLDVLPKD
                     ELSKEEVSDPILHCI"
     gene            13374..14960
                     /locus_tag="PLS88_02300"
     CDS             13374..14960
                     /locus_tag="PLS88_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU06214.1"
                     /translation="MKKTRIALVALILFATFVGTAFAADNTLYLFTPTTGATMTTKLD
                     HPWVNRGDLWKVLMFRSLFLPDPTLTTVSPDLASSYQISSDGLTYTITMKKGASWHDG
                     TPVTADDVVFSIQTALKAAQVNAIYTGVFSHIVGASDFIAGKASSLSGLTAKGDVITM
                     KLDAKVGNAISVLGQFAIMPKHLLKDVNPLEFQNASFWAHPIGNGMYKLEEFNPGNYA
                     VFVPYEKYDGPKPKIARIVLTAMADPIVAAQSGKLDLYNTNATDQISEFNKMKGFTAY
                     PVDITFYRYFVVNIKDANGVPNKAMSDIRVRQAIMYAIDTKAIAEGLYPGLAKLIYTG
                     VPTSHSAYDKSSEVYSYNPEKAKQLLKEANFDFSKPLKLRFYYADQTSINFMTAIAQY
                     LAQVGIKTDVQKFQGDATTELYKVKDYDIALKGLSAFGFEEWYGEYASDNVNFVNILG
                     KDGYFDAKVNELRETTDPVKRNQILVDLQKLEQKYLYKLPLFILQNIYYVNTAKVKTP
                     GVFGNPWYNWDYKFQDWEIK"
     gene            15080..16036
                     /locus_tag="PLS88_02305"
     CDS             15080..16036
                     /locus_tag="PLS88_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06215.1"
                     /translation="MLYYFIRKLLAIVPMLIIISIIVFVGIQFSPIDPISYLASPDMA
                     ANAANLEALREHLGLNDPIYVQYFRWFGNILKGDFGYSIVDGTSISKIIAMKLPATFE
                     ISLIALIISTILGIGIGIISAIKQNTIIDYIGRIIGVVGISIPQFFFGIILIQFFSIK
                     LGWLPIGGRIEFGYKTWWDHVPNIILPSLALGISMTAALLRYARNSMLDVMNKDYIKT
                     ARSKGIPEWKVYIKHAFRNALGPTLVILAFRLPILVGGSVMIEAVFSWPGIGAIILQG
                     VSASDYPVIMMTTLMIAAAMLLASFLVDLLMALLDPRIRFER"
     gene            16052..16915
                     /locus_tag="PLS88_02310"
     CDS             16052..16915
                     /locus_tag="PLS88_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06216.1"
                     /translation="MDQQRITKSSLALRSWNKLVRNKLAMIGTIVAIAMILASIFANF
                     ITPYSPSKIDFTNMTKPPSWKHLMGTDKMGRDLFAQVLYGGRISIYVGVVGAILGSAV
                     GMILGCIAGWFGGKIDAFLVRLSEIFLTFPNMILVLILVAFVGQGVNNLIFIFFITGW
                     MTPFRMIRNEFLSLREETYVEVCKAFGISDLSIMFKHILPNTISPIMVATTINIANYI
                     LQEAGLSFLGLGVPITVPTWGNIMNAARSIEVIRNYWWMWFFPGLVISIFVLAVNFAG
                     DGLRDVLDPNL"
     gene            16931..17944
                     /locus_tag="PLS88_02315"
     CDS             16931..17944
                     /locus_tag="PLS88_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06217.1"
                     /translation="MQPGRLILEVKNLQTNFRVGNEDISIIRGVSFSARQGETLAIVG
                     ESGCGKSVTVHSVMRLLPKNGRLTGGEAIFHDIDNNYDLHNLEPFGKEMRSIRGRKIG
                     MIFQDPMASLNPVYRVGDQVAENLLQHFKMSKKEARERIVEMFASLGIPDPARRIDDY
                     PHQFSGGMKQRVMIAIAMICNPDLLIADEPTTALDVTIQAQIIELMEGLKDEQGKAVI
                     LITHNMGLVAETADEVAVMYMGRIVERAPVERIFHNPCHPYTKALLRSVPVLGHGKTE
                     RLETIRGSTPSPSECTKGCEFANRCDFMMDKCRVLDVPVYEIEENHHVRCMLFEDSKE
                     LQQ"
     gene            17941..19026
                     /locus_tag="PLS88_02320"
     CDS             17941..19026
                     /locus_tag="PLS88_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU06218.1"
                     /translation="MNSQSYQQLPENCSQGNPGQEGIIFEIRNLKKYYPVLAGFFRKT
                     VGWVKALDDVSITIHAGETLGVVGESGCGKTTFGKTMMMLQHPTSGELLFHFPEGKRD
                     LAKLSSKELMPFRRKVQMIFQDPFSALNPSKKIFDSFDEPLRVHGHEDPKERKETIVR
                     NLEMVNLSAEYMYRYPHEFSGGQRQRICIARALCIGPEMVICDEPVSALDVSIQAQVL
                     NLMKDLQKQLGLTYVFIAHDLSVVQYMSDKIMVMYLGKIVEMADSISLYEMPRHPYTQ
                     ALLSAVPIPSIDVKKKRIILQGDVPSPIDKPSGCAFHPRCRDAMDICRRIEPEFAPLS
                     DDPSHFIACHLYGKTNGESEPTKKGKL"
     gene            19023..20483
                     /locus_tag="PLS88_02325"
     CDS             19023..20483
                     /locus_tag="PLS88_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06219.1"
                     /translation="MNKPKKYLLFDRGIIDGHLCQNAVLRLAKAEKDTKNNPMMTEEF
                     FANPSKPWEVRYDNGYPNVFRDIEQNVFRLYYTTFSYDKDSAQTRLEERPFRQYMPSK
                     SRATCTCYAMSTDGIHWQKPELGLSEFEGNLANNIILKNAHGTSVLCDRADPDPAKRY
                     KLITKMHYSQENNYMAVAFSRDGIDFSTPQPWPKYNPPADTHNFAFVDPKTNRYVLVT
                     RIWKDGIRIAAKSESSDFLTWTEPEEIARGIGFDSQVYSMPVFFYEGLYLGLASIYHD
                     GDRSAGNFDTVDLSLMYTFDLRHFDWIEAGATLIERGKGKYPDGDWDCGCIYASSPIK
                     IDDRICIYYMGGNGQHTNFRETSLGRAWIDKDKFAYYAAKESSKEATLTLRPANFYGE
                     HLEILADIEDDGWIDCEFIDVSNSSYFDEVASARGRKQVTRITKSGWNKIIFGNMSTS
                     YLAEKPLSLCLKFRNSRIYAIEGDLAITRQERLERG"
     gene            20494..21390
                     /locus_tag="PLS88_02330"
     CDS             20494..21390
                     /locus_tag="PLS88_02330"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003164.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HOU06220.1"
                     /translation="MKLESGIYAAQLVPYTSDGIVKEKSLSAMVERNITLGKVDGLYI
                     GGSTGESFLSDTNAKKKVLQIAAETNAGRVSMIAQIGSINVKEAEELAHLAAALGYDA
                     VSAVTPYYYRFNFSEICDYYFRLAEAANLPMLVYYIPAFTGTSFDIEQARRLFEHPLI
                     AGFKYTSGDLYTMERLISAFPDKIVFSGYDELLLCGKALGAFGAIGSTYNLFAHIAKE
                     IWKNVDLGLISQARAGQAKLNSAIQELTSLGLYQALKEIISISGIDTGCCLPPFSPLS
                     QEQKEKADALGRKYVDMKLIGL"
     gene            complement(21463..22590)
                     /locus_tag="PLS88_02335"
     CDS             complement(21463..22590)
                     /locus_tag="PLS88_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06221.1"
                     /translation="MTTAALELINVTKTFHKEREKVTAVDSVNLIVNQGEMVVFLGPS
                     GCGKTTTLRMIAGFELPSSGKILIEGSDMTNVPVNKRGIGFVFQNYALFPHMNVYNNV
                     AYGLRSRNERESTIKVKVKNALDLVGLTNEEGRFPNQLSGGEQQRVALARVLVMEPKL
                     LLMDEPLSNLDAKLRIYMRTELRRIQKKLGITCLYVTHDQSEALTMADRIMVMNKGHV
                     EQIGTPFDIYAQPSSLFVADFIGQANILEVIVESIEGNFALVSIPDGWHYTARIIDPS
                     RIRSGSKASLIVRPENIRVRKRQIERIDDIACEPKTENIQAIVNSTVFMGGHIEYQLD
                     LGSRGKLLAFEPFRPGSDIYQEGDNVCAIIDKDMALLLPGN"
     gene            complement(22571..24166)
                     /locus_tag="PLS88_02340"
     CDS             complement(22571..24166)
                     /locus_tag="PLS88_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU06222.1"
                     /translation="MLKLLATTFLVKGSFSLDNLTKILRKTYNQKAFCNSILLAAAVS
                     AAGTLIGYLFALAVTRTNWPKYFKWLLSSITILPLISPPFTSSISLTLTLGPNGILLK
                     ALHLDDLNFYGFWGTFLSETLTYFPVAFMVLSAILSTMDANIDDAALSLEGSRGRIFR
                     TITLPLSMPGIANSILLLFGSSLADFATPLILGGHNFPILPTQAYLQITGLYDLKGGS
                     SLSFLLILPALLVFLFQRYLVGKKSYVTITGKSGAQSQFSRLPFWLETLTAVICSFIA
                     LFIVYLYFIILQGSLVKVWSVDNTFTLEHFSYVFTNGKKAILDTLKIAIISTLIGSFL
                     AIIIGYIIQRKKFAGKRALEFISMLNYALPGTVVGIAYILAFNEKPILLTGTMTILVA
                     AYVFRYYATGIRSVVASLQQIDPSIEEASASLGAGSVRTLFKVTIPLIIPSVLAGMRY
                     LFIHCMTAISATIFLVSVRWTLLTTRILESMTELQFSQASAFSIVLIVLVFIADAIMG
                     GMMKLSTKKFGIAQENIYDNSGA"
     gene            complement(24408..25403)
                     /locus_tag="PLS88_02345"
     CDS             complement(24408..25403)
                     /locus_tag="PLS88_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU06223.1"
                     /translation="MKRFLLGLAFVLLVLPAMLYAQETLNVYTIMPEKYATQVFAAFS
                     ADTGVKVNFIRLSAGEALARIIAEKNNPQVDCLWGGPADTYEAGILEGIFEQYKPKEA
                     DKIPASFKSSENYWIGIGIIPLVFMSNGKFLKEHHLNPPTSWEDLLNPAYKNALQMAD
                     ARTSGTATERIYTLVKIYGEDGAFAYQKKLNQNIQLYTKSGAGGAMPIATGQAASGVF
                     YIVDALDIQQQGYDVVLSYPKEGVTYGVEAAGILKGAKNLTAAKKLMDWASSKRLGEV
                     MVDKKINYIPTRPDVKVTNPNLDMSKVKLIDADIAWKGKNRQAYVERWVNEVIEQ"
     gene            complement(25707..26630)
                     /locus_tag="PLS88_02350"
     CDS             complement(25707..26630)
                     /locus_tag="PLS88_02350"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOU06224.1"
                     /translation="MNKTIPDGVYPTLITPYEKNGRIDWNALETLIEWHIAHGVAGFF
                     AVCQSSEMFHLTLRERLELARMSIKFSRDRVPVLVSGHVADTLYDQIEEAKMMTDTGA
                     DALILVTNRLARPYEGNDIFKRNLETFLTAFDRDILLGFYECPAPYKRLLNADLVQFI
                     VETVRFGFLKDTSCRIGDMKAKIEAAKGSNFKLFNANAATLLASLQKGASGYSGIMTH
                     FHSDLYVWMCKHWNDQPDRALELQDFLGLASIIEYQLYPINAMYALQREGLPIKLISR
                     RADARLFTESMRLEVEQLMGLSHRFSEAYRE"
     gene            complement(26638..27936)
                     /locus_tag="PLS88_02355"
     CDS             complement(26638..27936)
                     /locus_tag="PLS88_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOU06225.1"
                     /translation="MNLPLIVFVLSFVAVFLLGIPLAPGMMLSSAFYFLLSKNPAASL
                     DMVAMQFLTNLNASFVLIAVPLFVFMAEIMNSGKVTEMIFSFSSALVGKRKGALAHVN
                     IINSIIFSGMTGSALADASGPGNMELKAMQQQGYDDAYTCAITAASATIGPIIPPSIP
                     MVVYAMLSSTSIGYLFLGGIIPGLLVGIALLIYNTFISNKRNYPEGKKFPAKQFWKFT
                     LQALPALFTVVILLGGIYTGVVTPTEAGALAAFYGILISVFVYRALGWKEFKQCLINA
                     VRTTGTLSLLVGSSFAFSYIVSIEQIPNVIATFLLGLTQNKYAMLLILNIAMLILGCF
                     LDTTPIQLIFTPIIIPILSHLGIDLVHFGVVMVLNLMIGLSTPPYGMLLFTVSGLTGT
                     PLKDIIREIMPMVIVLIVVLFLITYIPDLVLFIPKLSGYK"
     gene            complement(27933..28421)
                     /locus_tag="PLS88_02360"
     CDS             complement(27933..28421)
                     /locus_tag="PLS88_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOU06226.1"
                     /translation="MPYWLKKTGKFLVDCIELYVPILTFSTLFVSFVLQIFARKFFKP
                     LLWPEELSLICFVWTALLGGLYSKRMGTQVAFTMLYDAAKPKTKRIMRIVGNAMLLVA
                     FLIILVPSWDYVQFMRYKKSDALGIPMNIAYFPFVVYLVDMIIRLTIDIAKDFSISGD
                     TK"
     gene            complement(28531..29529)
                     /locus_tag="PLS88_02365"
     CDS             complement(28531..29529)
                     /locus_tag="PLS88_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HOU06227.1"
                     /translation="MKKTIVAMLVLLMIASPTVFAQQKPIKLVFTSVSVPTDAHTQAM
                     YVFKEEVEKLSGGQIQIDVYDSGKLFTQQAEQDAIRKGTVDMVYSSASWLAEFVPYLS
                     MFGAAYTFQSYDQMTKTFNGPIGKKMFEDVAKKTGVRPLCAFYLGTRQLNLIAKVGPV
                     TKPEQMKGVKLRVPQSPTWIAMGKALGANPTPMAFNELYMGLKTGAVDGQDNPLPTDK
                     NAKFYEVTKYIVLTNHLVDSVWPSINEKKWQSLTKQQQDWVLQALEKARQSCDKTNLD
                     NEKEILDFFRQQGLVVIENPDRDAFAAYAKNFYLTEGKDISKDWDLALYDEIQKMK"
     gene            complement(29658..30590)
                     /gene="dapA"
                     /locus_tag="PLS88_02370"
     CDS             complement(29658..30590)
                     /gene="dapA"
                     /locus_tag="PLS88_02370"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOU06228.1"
                     /translation="MAKFVKERYGRILIPLLTPFCEDGSVDYERTAALAEFIIDQKKG
                     DSLVLSGSTGEFYAMSFDERVRLFETVKDKVGDRIPIIAGVGAASTIETIQLAKIAEK
                     IGFETVMIVAPYYTKPNQEELYRHFMQVATSVSLNILLYNIPIYTGVNIEPTTVSRLA
                     KQPNIVGIKEEAELNPKQITAFLNSTPENFVIYNGDDTMILEAYAQGGEKRIGGVVSG
                     SSHIIGHLIRSMIEDFLAGKVQAAAEQQQKIYPFLKSMGQNKRTNAAPLLKEALRLYG
                     FDAGYPRAPLMCGTPEEVENIRLAMIQLALFKER"
     gene            complement(30620..31399)
                     /locus_tag="PLS88_02375"
     CDS             complement(30620..31399)
                     /locus_tag="PLS88_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOU06229.1"
                     /translation="MAQVNSVRKALAILDLLSDGEKRSLSDIARELSLAKSTASNLLE
                     TLTAERVLERNRESGFYSLGIRLIELGYCTQTGHELVRIAAPFLRGLNQRFDETVHLV
                     LLDRDEVLYIDCIESHQRLRTYSVIGVRAPLHCTSVGKAILAFLPDSEIRRIIIRKGL
                     PSFTSNTITTEERLWKEIELIREQGYAIDDMEHEDHLRCVGAPIFNVEAKVFASVSLS
                     GPAERNTLKRLESIAPALLEATTEISHRLGYRNKDLSRLGS"
     gene            complement(31575..31937)
                     /locus_tag="PLS88_02380"
     CDS             complement(31575..31937)
                     /locus_tag="PLS88_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06230.1"
                     /translation="MTIPHIEAIMPAWQDEFGASITVIGCNYEILYMNEKSAMSNAKW
                     GGKALIGQDVRACHQQSSVRLIEHILETGEPHTYTIEKQGVKKFIYQAPWREQGSIAG
                     VVELSIEIPAEIPHYIRS"
     gene            32359..33306
                     /locus_tag="PLS88_02385"
     CDS             32359..33306
                     /locus_tag="PLS88_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU06231.1"
                     /translation="MKRVLSVFIAMLLIAGTIGTVGAQAQKRYKFVVITHATAVPFFV
                     PVRKGAEDAGKLIGADVVYTGPTGFDIQQQVEFVKSAIAQKVDGIAMTMPDANAFNAV
                     VKEAMSKGIPVIAINADAPESGRLAYIGQGNYEAGRSMGMQIVKLLPNGGDVLLTTHT
                     AGAENLEDRIRGIKDVLDAQGGKFKYRVVATGTDMVRAVSLISSALQANPNIKGMFGV
                     EEVTGSAIAQIIERDRLKGKVMAGGFDLVTDELVAIQKGNMQFTIDQQPYLQGFQGVM
                     ELYLLKKFNLTPCDINTGIAPVTAENVNEVMKLAAEGYR"
     gene            33409..34899
                     /locus_tag="PLS88_02390"
     CDS             33409..34899
                     /locus_tag="PLS88_02390"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU06232.1"
                     /translation="MQSLLSVQNISKHFGNIHALRNVSLDISPKEIIGLAGENGAGKS
                     TLVRIICGALSYDSGEMSYQGKPYKPHDVSNAESLGISVFHQEIPICPHLSIAANVFL
                     GPMIPSQRGRPDWKLMNNRCIELYRKFLDEDIDPTVPIGECSAAEQQLALLVRVLSRN
                     AKLVILDEPTTALSAPEVERLFDSIRRLKDEQGIGFIFVSHLLDELMELSDEIFVLRD
                     GENVGHLYKAEFSAKELSRLIAGRSIDERHRIAKDISDAEIALEAKSISLSKSYEDIS
                     FSVHKGEVFGIAGLQGSGRSEVISSLFGLPPPDKGSVVIRGNDFGRITISRAIAAGVG
                     YLPEDRKTKGIFPDLNIAKNIGIVSISQSKDLTGYNEEDYIKVANNMATKLSIKISNP
                     RDYISALSGGNQQKAMLARWLALSPSILVLNEPTRGVDIAAKAEIAKIIFDMAGAGCT
                     IIMASSEMEELLIMCDRILVMNRGRAKIILEKESLTKENLFFYAMS"
     gene            34917..35903
                     /locus_tag="PLS88_02395"
     CDS             34917..35903
                     /locus_tag="PLS88_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06233.1"
                     /translation="MNKSATKNQKNKSRIVLGRDIGVLIAAILIAVFFTIASPVFLTA
                     YNFFNLLRQTAQLGIIAMAMTMLIISGEFDLSVGAIYAVTGVVTGILVKNMGFNIWPA
                     ALIGFLTALLLGFVNGTLISTTKINSFIGTLSTQMIFRGIAMVLSQGQPISFLQSSIF
                     FDAFGRAELFGNIPVQIFWLLAWTLISFYLLHRTSFGVKVFATGDNRLAADLSGIKTM
                     SIKKTCFMITSAAAGLSSLISLAFLKTVTPTQGSGLELEAIAAGVIGGTSMSGGVGSI
                     LGTFIGTFIMAEVRTGLVLMGTDAYVQDAFVGLVIALAVIINVRFSISKDRT"
     gene            36010..>36498
                     /locus_tag="PLS88_02400"
     CDS             36010..>36498
                     /locus_tag="PLS88_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU06234.1"
                     /translation="MNFTIRAIAPVEAEDYYSLVNEVKDEGKFLFASIRIPLENMRKY
                     IAQHYESSNPVLGAFDDKKELLGWIDINRSNIEELAHIGTIAMGVRKSFRGQGIGGAL
                     LDACIHQARELGLEKLELEVFGSNSAARALYKKKGFFEEGIRLKKRKFEGHYDDLVCM
                     GMF"
BASE COUNT        10274 a         7605 c         7936 g        10683 t
ORIGIN      
        1 ttctgttggc gtgtatctag gcgcccatat tgcgctacaa ctggaacatc tgcgttgagg
       61 cgaagggcat actgagtttc gcgtggtagc tggataccaa tttcctcttc cttgtccaat
      121 cttaaacatc gaacgcggcg agcttttacg gtgagcgctg gcaagctgac cggttcttta
      181 tccgtaaaat agagtgtaat gatgatagat gcatcggaat cgttcatgtt tagaacaata
      241 agggattcat gtcccttgag cgggaaatcg cccggcggcg gtaattcgag atctggatag
      301 acccattgtt ttttgccctt atctatcggc tggtctttca tggtttctcc tattgctcct
      361 aagttttgca aatcatgctt gataggtgcc tattagcgta tcggcgacag aagcatattt
      421 gcccattagc tttcagccca catttgacag gcggctttta gtgcatatta ttggtacatc
      481 ttctaatctg tctgcgaagc caaagtcgta gcgaagcgtt gcattacagc cggcagggag
      541 gtgcttaagg ttgccaagat tcttctcatt tgaatagcct gtagggcctg ctgttgaagg
      601 aagatggaag ccgcaagcgc cacgatcttc attatgggtt atccaaatga catggtgatc
      661 gagctcgggt ttctgctggc gcacccagcg agcggttcca tcctcatgga gtagaatcga
      721 accagcctca gaagcaatat ctctatggtt caggattgcc actagttcgg gttcgtattt
      781 cgccttcttg tctatttgct tgataagaga tggatcggca aaaagagctg cagttttttc
      841 ttctccatca gctctgatag tgacatgtga agagtcaaaa ggtaccgttg ataatatctt
      901 ttcagcccca gaaaaggcga aatttatatg ggccaaatac atatattcca tagttttaga
      961 agcagggtta ttgaggagca cttccatact tgcctgcaag ccgtccgccg atatgtttag
     1021 agagggactg caatgatatt ccgctacaaa gggcactttc cagtggatat ggccacaaag
     1081 cgaaacagga ctttctgaac cgttttcagg ctccatttca atccatgcct cgtcgaattt
     1141 cgctaccggc aattctccat gatggaggtg agtgtcctct ggccctgggt ttcccattgc
     1201 cgtcattccg cagtgaatca agaatccacc ataattttgc aaaaaattct ttccataaga
     1261 tggctcttcg ataaaacctt cgaatttgag gcttttgcca tcgatgctcc aatcccatag
     1321 ctgctggcct agatatggca gccagatgaa ttcgccacgg cctatcttta ttcgtagtgc
     1381 ctctactcca gttctatatt ggaataggct cacgcagaag gatggagaag caagaatctc
     1441 gcgctctttc tccgagaaca tataaggatc taacttcatt atgatgggtt tgttctttct
     1501 catagccaca accaccccaa agcctcaatg ccggaacaga ttatttcatt gtcgggattc
     1561 tcagggtgct gtatgttatg gattattcgc tttatttcgt ggtaagggta catgcagctc
     1621 tttactcccg attcgcttac aggattgatg tcgaagaaca tatcttttac tagatcgaaa
     1681 tctttttcat tgtaatagag aatctcatct aggaggcgct ccttttcttc tgccgtagtt
     1741 gctttctcgt attctagaga agcgagcacc atgtcataga ttacgccatt tcttcgttcg
     1801 gcattttcta ttcgcgcaaa atgtggaata tactcggtaa gtctagcgtc tttttccagt
     1861 tcgcgctgga ttgcatgaat ttcttccaaa agaccaggat gcctcagctt tgcctgcatt
     1921 attagtggaa tatctgcttc cgtgattttg tttttctgga aagggaaagg cgtcgtataa
     1981 agaggcattt gactttcgtg gattgtgagt atgcttttaa gcactagaag gactctttgt
     2041 ccgagctcct ttccgaaact tgcctcgcaa gaacgacgga agaaggtttc tttatccatt
     2101 ctagagcccc atccatagtt cccatgggcc cattggaaca tgaaaaggcc ataccactcg
     2161 aacatgaaac catttatccc actgactcct tcttctgcac tggttatatg ttgttcgata
     2221 tctcgctcta taacttgaac gttcgattcg tcttcatagc ggattatatt agactcgaac
     2281 atgcgaaaaa gccgccccat agtggaagtg atggaattgg cttccgaatc tcttccccag
     2341 attctctctt tgccaaaagc ctttatccac ttcttgaatt ccgtaggagg cacatataag
     2401 gctggcgccc aaaatagcac aacatcatct ggcatacttc ttacgcactc ttcgagaaat
     2461 acagggtctt tttcgagagg aggttgtcta aacgcacgaa tgccgattac tgcttgtggg
     2521 tttatctttc gtatctcttt gtagatcaga tttagaagcc acatgttggc tttatgtttt
     2581 gcttctcctg gggaagccga ggttttgccc catcgctctg tgcatttttc gcagttgcag
     2641 aaccagaagc cttcctcgct ttcttcgaga gtaaaaccat cgaaaccaag ttgggcgatt
     2701 tttctcatgc tggcaaggat ataagtgatg ttatcttcat tgcttagaca aagcgagtcg
     2761 aagtcgttca taaagttcga gccggcaggc ttaatcattc gcttttctgg atgcttaatg
     2821 ctgtaagcac cattatagga atttagtccg acatacgcga atatcttaaa atcgaatcta
     2881 tgggcaagag taataaagtc agctagaaag ttttgctcga tattggggtg agagtaatgg
     2941 atagtcagcc actgactatt ctctttgttc cagatcttta cagggatatc cttgaagact
     3001 gtttctgggt attcttcgaa gtcgaatggc caatatccat acatgaccat gttcatttga
     3061 tttattttgc aatcgataca agaattgaga tactttaccc attcttcata gcaccatagt
     3121 tgcatgtcaa agcccagccg accatagccg gcataccatg aaaatgtcgg agctacagcg
     3181 cgatgctcga tcgagggcca atccgttatt tcgcatagtg gaagcagata catctcgtct
     3241 tttttgatta gaagttgctt tatggttgta atagcataga tgaagccgga tttttcctgg
     3301 tatctaagaa cgattcgatt tttctctatt tttagaaaat atccttgttt tataaaaaga
     3361 tcgcggttgc ttgcctctat acccgataga ctttgaatgg aatttatggt gatggattct
     3421 tcgtttgatg tatctgcaaa aattttgaca tctttgcagt tccagaatct catttgagca
     3481 atctctaata ggtccgattt ttctgcttct gacaggttcc caaaatcgat agcaagagcg
     3541 tggaaagcct ttgtataacc atcttgttct tgaagctttt ttggttcagg gaaaagattg
     3601 atcatggttt tacctcctct tattagccca aaaatcctcg agggattcta gaagggcttt
     3661 atttctcata tcgattctat gcaaataatt tcgccaaatc tcgcgcagtg acaaactccg
     3721 ctcccattcg agcaaggcca tctatttgtt tttccagctc gcaaagtgca ataggcccac
     3781 agttctttgt tataaactca tctggaatga ctgttgcctc gccgaaatga tagcttgttt
     3841 tcattggcca gaattcccat gggtggatgt aatacgcgag aacggcaggg attccttttt
     3901 tgttgcagat atcgatgaac gaggcgcttt tcttcaaaat gtactctgct ccttctgtcc
     3961 tgaacaaagg ccattggtct ctatctcgtt ccagtccagg atctttgctt tgcattgtca
     4021 tatcggcaaa attaggaact tcgagaacag aactgtcgcc cttttttgtc cagtcagtcc
     4081 tatctggatg atatggagag aattgctgcc ggtaaaagta cattgggtat gaaacatcgc
     4141 aggtaaagcc aagctcttcc agtgcgttga ctactgccgt cgagccccag aggcgaggac
     4201 aacgaaatga agtggtcttt acgccagcag cttcctgcac ccagtctctg catagttcaa
     4261 gtcgtggttt tacttcgtgt gaaagaagag gatatacacc agggatcgga aagagttcat
     4321 ctcctattgt ttcatgatat agcgaatgac atcctacttc gtttccacta tttgcaactg
     4381 tttttgcgat gtcggggttt ctacgtgcgc aatctcctgt aaagaaaaat gtgcctttga
     4441 tgcctttttt ggcaaagaga tctagcaaaa gcggagttcc atttcgcgcc ccttcgtaga
     4501 atggcgtaaa gctgccgaca tcggtttcca tatcgattcc aaacaaaaca tggaatgatt
     4561 tcatttcgaa ttctcctcga aaatactaga attcaagctc gtataaggtg ccaataggag
     4621 gcgacaacac ttcgactcca ttttctttga ttgctatgcc tttttcccag cgtactccaa
     4681 atgtgtctgt cgaaataaag gtgtcgatct ggaaggtcat attaggctta aaggcataat
     4741 ttgatgtcgt ttccacccaa ggcgcttcga cttcgattaa ccccgtacca tgcaatgggc
     4801 cgtagacaaa attatttgca tatccattcg atacatagaa ttgataaaaa tcctttgcaa
     4861 catcggcggc catgacccct tccttcagct gagcttctgt ccatctatga gcttcgagtg
     4921 caaacatgac gatatcgcgc cttttgcctg atagtttgcc aaggacgata ggatagccta
     4981 tcgaggccga gtagccatcg atttttgcag ataggttgag ctgtacaata tccccttttt
     5041 cgaatctacg ataggaagac ctcgaaatag catgccttgt cgaagcttct gagaatacat
     5101 acataggcaa accttcgtac tcggcgccat tttcgtatat tgcgcgttga gctacgccta
     5161 ccatctgaag ctcggtcatg cccggcttga tttccttgat tacctgctga cttgccagct
     5221 ctgcgagacg ataaccttcc tttagacagg agatttcgtt ttcggacttg atagagcgaa
     5281 gttctaccat caaatagtcg gcccgaacga tctcggcctc tggaaaagct tttctaatgc
     5341 cttccattat gacaacggaa gtgtccagat agcttgcgac tcctattctc agttttttcc
     5401 catttatgcc aaggcctttg aaaacatcct tgaatgtatc agccttgagt tcaggatagg
     5461 ccggatcggc gctttctctg tagtctttta aaacatatag cttgttcaat ttattgcgat
     5521 caccaccaaa agctctgctc tcgggcccga ccatgagagc agcatcccct gctgcaccta
     5581 tggccactcc gcagcgctcg aaaagaggcc agaaacctga aaaatacctt gtattagcat
     5641 agtctgcttc ggttccattt acgattagag catctaatcc atctcgctta agtagcgctg
     5701 ctgctttgct tacacgttct ttgtattcat agtctggtat gtggatattc ccatgcatct
     5761 tcatttctcc tctacactag ctattcttgc tttttgagat gtagttacaa cttctctctt
     5821 ggtgttggaa tctattagta cgacgatgat taggatgatg ccctttacaa cccactgcca
     5881 gtaggtattt attcccatga gcaccattgc gttgcttatt acttggagaa acagagctcc
     5941 aataaccact ccaaagatcc gcccacgtcc accagttaag gatgtgccac caagcactac
     6001 aatggtcatg acatcgaaca acaggttttc gcctaaagac acattgcccg ataatgtttt
     6061 ggccgtaagg cccagagcgc cgactccgct caacacacca gaaaaaacaa aggccaagat
     6121 acttacgaaa actacattga tgcccgaaag tttacttgcc tcaaggttgc cacctactgc
     6181 atagaagcta cgcccaaccg cagtatgcgc ggttattatg aacaggataa ttgcaacgat
     6241 gaacattaga ataaccggga agggcacaat tcctatgtac ccggaaccta gaaaattgaa
     6301 accatcgggt aggcccgaaa tagggagccc tctggaatac aggtagttta tgccttttat
     6361 gatattcatc gtgacaagcg tggctatgaa cgatggcatc ttgccataaa cgaccaggat
     6421 tccattgata agtccagcca atgcagacat caataatacc aatataattg cgatccatat
     6481 tggcatcccc atttttacca caaatcctgc aaagaatatg gagaccatgc caatggtaga
     6541 agatatcgac atgtccattt gtccaatcag tatgacaaag aattctccca tcgctattat
     6601 tgcggtgaac gcggtttgcc gtagaatgtt cattaggttg tctgatgttc tgaatgtgct
     6661 aagggtaatg ctgaatatga caaccaaggc tatgagaagg aaaagcatta tccaatctct
     6721 aataaatgac cgccagcttt tcccatagcg tttcgatttc tcttcaaagg tactcatttc
     6781 ccatttactc ctaacagact gtggataatt acctcttgtc tttttctttc gccttctaca
     6841 gtatccataa tcataccttc gtagatgata tagattctgt tgcacaagcc gatcaattct
     6901 tcgagttccg gagaaactat gattacgctt tttccttgtt ttgtgagatc ttttatcaat
     6961 ttatagattt cgaattttgc cccgacatct atgcctctcg ttggttcatc caagaggaat
     7021 atctgggaat tctgaagcat ccatttagcg atgactattt tttgctggtt tccgccggat
     7081 aaagtctcaa cttcttgaaa aagcgaataa tagtttagcc caagtttttc gtttatatca
     7141 aaggcagctt tagcctctgc cttgtgatct tggaaaaatc tcattttctt gaatttattc
     7201 attgagggga ggatagtatt ttctttgatg tcgaatttca ggatcagtcc aagacttctc
     7261 cgatcatctg gtacataccc taagcctagc tgaatagcat gccaggggtt ctttattttt
     7321 acttccttgc ctttaacaaa gattttacca cttattactg gatgtacacc aaagattgct
     7381 tgtattaatt cagtcttgcc cgctccaact aaacctgcta gcccaattac ttcgcccgat
     7441 cttacataaa aatcgatatt cttgaatgct tttggagcag ttagcccttt gacttcgagc
     7501 ataatgtctc caaaatcagc attaatagat ggatagacct gtgtaagctc ccgtccgatt
     7561 atcattttga tgagtttctg ttgatcaatg tcgctaacat ttccactatt tatatggcga
     7621 ccatcgcaga ggacggtata agaatcacag cactcgaata tttcattcag tcgatgagaa
     7681 atatagataa ttcccattcc tcttttttta agaatgtcta ttattctgaa aatattttgt
     7741 atatcctttt cttgaagaac cgaagttagt tcgtctagga tgagcacctt tgatttagat
     7801 tgtactgctt tggcaagctg tatgatctgt ttttcggctg tgcgcaaata atggaccgga
     7861 atattgagat ttacatcgca ctgcaagaaa tccatcagtt ctttggcctt attgttgaga
     7921 gccttccagt ctattaggcc tccctttctg gaataatgac caatgaaaat attctctaag
     7981 cccgtcatct cactcatcag ctgaaattct tggtgcacta tttgaatgcc aaggttttga
     8041 gattctttta tggaataact ggagagcttg attttttgcc cttcgacaaa tagctcgccc
     8101 ttggtcatac ggtattcgcc agttatgcat tttgaaagag tcgacttgcc agcgccattc
     8161 tctccgacta ggccatggca ctccccgggc atcaaagtaa aattcacatt atccaatgcc
     8221 ctgacacctg ggaactccat tgtgcagttt cttagttcaa tcaatggcac tgttttattg
     8281 agctttgggg tatcatcagt gtcagattga aaacgatttg tcattgccat atctcccact
     8341 atctcttatt tatctagtat ctaggtggct ttctcgataa tgcctctttt gaccttataa
     8401 aaagaagagg cgggcaagga ttgtctccct gcccaccccg tcctcatagt cttttcatgg
     8461 gcataaatag gtcgtttata gccaagacca actttgccaa ttgtagttct ttatatttgc
     8521 aggagtgatt gcctcaggca aaggaagatt gtaggtttcg ggaagctttt cgcccttcaa
     8581 tgctcggtac atggcatcag tagcttgata tcccaatact actgggtcct ggaggacctc
     8641 acctatccat gctggattcc ctttggaaat gaaagaaagg acatctttat ctccagctat
     8701 gccgatgaat tttatattag ttcttttctg tgagaccgcg gcgttatatg caccgacaac
     8761 ttctgcatcg ttatgccctg atattacatc gatgacaggc tctcttttaa gcacattctc
     8821 cactagatcc tgtgaagaag ccctattccc agcacccaca tcgccttctt cgatgacctt
     8881 ggctttgggg aacgaggcaa gcactgtatc tctgaagccc tcgaatctat cttttgaaac
     8941 ctgtccagag gtgggacgag atacgaacag acatacgggg ttgggcttat tcaatgtctt
     9001 taggtaatca accgcgattt ttccggcaac gacacccaaa gctctcatgt catgaccaac
     9061 gaatgttgcc catacagcat cagatggttt ggctggctgt tgtttataat cgccgattac
     9121 aaaaggaatg ccttttgcaa cagcttgctt catggtggga attgccgaag aagcatccgc
     9181 tgggaacaat atgattccct ttgcgcctgc agcaagaaga ttttcgatgt tgtttagcat
     9241 tgtctgcaca tcggaatgag aatcttcgaa ttgaaatgta aaattcgcct caggatgttc
     9301 ggaagcccat tgcttgcaac catctatgac accaaggaac catgtataag cagtaagctc
     9361 cattatgctt acaccgataa gggtcttgtt gtcttttgtc tgtgctggtg tcttctgagc
     9421 ccatccagcc gtggatgcaa ggagaaaaat gattagcatg attgaaccga tttttttcat
     9481 agtctcctcc ataaagatct accaaagact tattgccaac gttgtcggca acgattacaa
     9541 tcatcctatt gaagtgtgaa tttgtcaagc ttttttttaa gaatgtcact tgagtgaggg
     9601 tttggaagac ctaggactta gaaggctgag gatccagaag accgaaaatc tggaagacaa
     9661 gcggcatgtc ttgaacaaat aataagggga aaatattttt catattagag agttattgat
     9721 atagagagct attgacattt gccaacgttg tcggcaacgc taggtgttgc aaaacatctc
     9781 gtatatagag caggcaagac aatggagcgt taagccatga caaaactaaa agatattgcg
     9841 gatgaaacag gattttcaat aaagacagtc tctcgcgcta tacatgacca tccagatatt
     9901 aaaaaagaaa cgaaagaaat cataatggaa gtagtccgaa agcgaggatt tactcccaac
     9961 tgggctgccc agagcctcag gaatcagaaa acaaatacaa tcggctatat cattccaaat
    10021 ataacgaatg gattcttcag tgagatcggc atagccatcg atgcgttttt tagagagaaa
    10081 ggttattcta cactcatttg ttttacatcg gactcaaggg aaaacgagat tgaatcaatt
    10141 cgctctcttc ttgcaaagaa tatcgatgga attattctcg caccgatagg aaacagagga
    10201 gaatttatag acgagatacc aaacctcgcc gatacgcctc ttgtgttgat cgacaaccgg
    10261 cttagcgggc cacctcgcaa ttatgtgctt caggacaatg aaggcaattc tcgcagacta
    10321 actgcccata ttattagcca tggccatgcg aatataggtt ttgtgactgg tcctctagag
    10381 gaatcatctt cccgcgaacg ctttttgggt ttctgcaatg ctctggaaga tgcaggcgca
    10441 atatacgacg agagccttgt gcgaaccgta gactgggaaa tccacagtgg ctatgaggct
    10501 accaaggatc tcttcgatca taaaggaaac acgagaccaa ctgcaattat atacggcaac
    10561 actcagctcc ttcttggcgg atataaagca cttcgagaga aagatctttt gattcctcag
    10621 gatgttgccg ttgcctcttt tgagcatcct gacataatcg atgcgcttac tccctgtccc
    10681 actacacttg aaaaggtcga aaagagaata ggcatagcag cagctgaaat gctcttgtct
    10741 cttatcgaaa caaaagaaga aataccacca caggaaatcc ttatcccaag ccaactgata
    10801 ataggagaat cctgcggctg ctttgctaga agccaaaatc cgtaatctta gatctataac
    10861 aaaatccatt gagaaaaaat ctaagtctag ctcaatctat tatttcaaag tcaaatgtgt
    10921 aattgtagat atgattttga gttaaaccag cttcctcttt tctctttaat accccaaata
    10981 gtctcttttc ttccagagat gctgaatcac gaacttttcg ccttttggat ctcgcgcctt
    11041 tactagattt ataatgcgcc agtgctcttc ctgggttttt cgagctattt cggggttcat
    11101 ggtctgcagg aaaaggtgcg aagacttgag attgagcctt tcgaagaagt tttcgagcaa
    11161 ttcattgccc gacattctta tgaattctct atggaatctg tagtggactt ctgcgtaggt
    11221 catcatgtcg ataggagctg tagcaatgat atcgtccatt gttttattta aagcttccat
    11281 ctctgatgta tcggctccgc gctcgactga gagcctaaaa gctaaccctt cgagataagc
    11341 ccttacttct atgagatcga ggtagttctt gcctgaataa atgcgaacat agctgcctga
    11401 ttttggtttg acaacaatta ggccatcttg ttctagtctt ttgaacactt ctctgattgg
    11461 cgttctagag cattcgaatt cctttgccag agcgttttcg gaaagttttt gtccggcaaa
    11521 ataatactca tcaagaatgc gtcggcggag aactgaatag agttcttcga cattattggc
    11581 gatatctgtc atgctgtttc gggtctagat ccctcctata tgcaatgcag gattggatcg
    11641 gaaacctcct cctttgagag ttcatccttt gggagtacat ctagcaagct gattactttg
    11701 agcatgcaac gcggaatatg aaagcatact ttataatggt ttccttttac agtatttgct
    11761 atggagccat cgcgatgcag gtatccgaac cattccccat agactgggtc tgggaaatga
    11821 gagaatgtat attcatggat cgtttcgaac catcgttccc agcgcctgtc ttttgtggat
    11881 acataagcca tcagcgtggc gattagagcc tcacaatgag gccaccaata tttcatatcc
    11941 cattctatct gtgcaggctg ttttccttcg gcatctagga aagagaacaa gccaccaaac
    12001 tcggtgtccc atccgcgctc gagagaccag gaaaggagat cgatagcttt attgagtaga
    12061 actttatcac cattaatgtg agcttcgctc ataaggaacc aggaggtctc tataatatga
    12121 ccggggttaa ttgtgcgtcc ttcgcatcct tccgtcattt ctccatctgg atctactgtc
    12181 tcgagcagga cttttttttc gggctttacg aaataacgga aaagctcttc tatgcgatct
    12241 tgtattctgc ttgagtaaat agaggcatgc tctggatctg catctctaag aacttgaaag
    12301 acatttatca ttatcattgt atcggaatgg cctctcattg ggcgcagcgc tggattgatt
    12361 ttcggctcga gctttcctct gaagcggtcg tagactgcta gagtgtctat tgcttttcta
    12421 aggtaggatg aatctccgga tgctttggaa tactccgcga ggccgataat tgcgaatgcc
    12481 tctgaaaata gatatcgcct aagtatcaag gggtttccat tccgatcgag ttcgaaataa
    12541 gcccgacctt cggggccagc aagcacttta tctcttgtaa aatcagcaca cgatcttgcg
    12601 gcagagagcc aagtatctct aggttccaga tcattataga gagcagagaa tatccaagtc
    12661 gcacgtccct gtgcccatcc acctttatcc gtagaaaggg gttgtccctg ccgatctaga
    12721 aagtctaaaa ggccgccttt ttcattgtct attccatgag aaagccagaa aggcaagata
    12781 tcatccgtta gctctcgata ataaaaattc ctcaattcat gtatatgcga taccgtcata
    12841 gcagtttcct cttttgattt gggcaatggc aattattcaa tattagcaga tagagacaat
    12901 ttgaacaggg tttgcacatt tgaaatacgg agcaaaatgt aagcgctttt tttggttcaa
    12961 gtaatccgga tttaattgga attatgcaca taacatgtat actactttga atattatccc
    13021 atgtcaacga aaatatgata tctcgctaga aagcaggtcg atttaataag gataagaaat
    13081 atcaaacatt agaagatatc tttaagtcac taaaatataa taataaataa cattttttat
    13141 tatattacac aaagcgcata atcgcgattc atctcaaagc gcaaaataaa ataatagtag
    13201 aaatcttgta tgcttgatta aagaaaaaat tttaaagaag tatgcaatat aatcttgaca
    13261 atttcaaaat ctggatacat tctattattc gagatctaag ttcgctactt agaataatta
    13321 gtctaaaaat gccttgatcg tcacggcgaa agtaattttt taggaggttc gatatgaaaa
    13381 aaacgcggat cgctcttgtc gcgctgatat tgtttgcgac ctttgtagga acagcgtttg
    13441 cagctgacaa tacgctatat ctatttactc ctacgactgg cgcaacaatg accacgaaat
    13501 tggatcatcc gtgggtaaat aggggagatc tatggaaagt gctcatgttc cgcagtcttt
    13561 ttttgcctga tcctacgctc accacggtat cgccagatct cgcttctagc taccagattt
    13621 cttcggatgg acttacatat acgatcacta tgaaaaaggg tgcaagttgg cacgatggca
    13681 ctccagttac cgcagatgat gttgtattta gtattcaaac agcgctaaaa gccgcacagg
    13741 tcaatgcaat atatacaggc gttttttctc atatagtagg agcgtctgat tttattgcag
    13801 ggaaagcctc gtccctttcg ggacttactg cgaaaggcga tgtaatcacc atgaagctcg
    13861 atgcaaaagt cgggaatgct ataagcgtgc ttggtcagtt tgctatcatg cccaagcatc
    13921 ttctaaagga tgtcaatccc ctcgaattcc agaatgcgtc attttgggct catcctatag
    13981 ggaatggaat gtacaaactc gaagaattca atcctgggaa ttatgcggta ttcgttccct
    14041 atgagaaata cgatgggcca aaaccaaaaa tcgcacgcat cgtgctaacc gctatggccg
    14101 atccaattgt ggcggctcag tctggcaagc tagacctcta taacaccaat gctaccgatc
    14161 agatatccga attcaataaa atgaaaggat ttactgcata tccagtggat atcaccttct
    14221 atcggtattt tgtggtgaat atcaaggatg ctaacggtgt gcccaacaag gcgatgtcag
    14281 atataagagt gcgccaagct attatgtatg ctatcgatac taaggcaata gccgagggtc
    14341 tctatcctgg attggccaaa cttatctata ccggtgttcc tacgagccac tcagcctatg
    14401 ataagagtag tgaggtatat tcttataatc ctgaaaaagc taagcagctc ctcaaagaag
    14461 caaacttcga tttcagcaag cctcttaagc tgcgtttcta ttatgcagat cagacatcga
    14521 tcaattttat gacagccatt gcgcaatacc ttgctcaagt aggaattaag acagatgtcc
    14581 aaaaattcca gggagatgcg acaacagaac tatataaggt aaaagactat gacatagcac
    14641 ttaaaggtct atctgccttt ggttttgaag agtggtatgg tgagtatgcg agcgacaacg
    14701 tgaattttgt gaacatcttg gggaaagatg gttattttga tgcaaaagtc aacgagcttc
    14761 gcgagaccac tgatcccgta aaacgcaacc agatactggt cgatctgcaa aagctagaac
    14821 agaaatacct ctataaattg ccgctcttta ttctgcagaa tatttattat gtgaatacgg
    14881 caaaagtcaa gacacctggc gtattcggca atccttggta caattgggat tacaaattcc
    14941 aggattggga gataaagtaa aataataaat tatattcgat gttttttagt aattggcttc
    15001 tagcgatgtg tgtgagggct tgtcccttgc acacatcgtt ctttagagag tgatttaatt
    15061 ttccttggga attttggaaa tgctatacta ttttataagg aaactgctcg ccatcgttcc
    15121 gatgctgatc ataataagca ttatagtttt tgttggcatt cagttttccc caatcgaccc
    15181 tataagctac ttagcttctc ccgatatggc agcaaatgct gctaatctag aagcattgcg
    15241 ggaacatctt gggctaaatg atccaatcta tgttcaatat tttagatggt ttggcaatat
    15301 actgaaagga gattttggat acagcattgt cgatggtact tccatttcca aaatcattgc
    15361 aatgaaattg cctgctactt tcgagatttc gcttattgca ctcataatat cgaccatttt
    15421 gggtattgga attggaataa tttcagctat aaagcagaat acaatcatcg attatatagg
    15481 ccgaataata ggtgttgttg gcatttctat tccacaattt ttctttggga ttatcctaat
    15541 tcaatttttc tctataaagc taggctggct gcccataggc gggaggatag agttcggata
    15601 taaaacatgg tgggaccatg tgcctaatat tattctgccc tctttagctc ttggaatatc
    15661 tatgacagct gctcttctca gatatgcacg caattcgatg ttagatgtaa tgaacaagga
    15721 ctacataaag actgcgcgct caaaaggcat tcctgagtgg aaagtgtata tcaaacatgc
    15781 attcagaaat gccttaggcc caacattggt tatcctcgct ttccgccttc cgattctagt
    15841 gggaggatcg gttatgatcg aggcagtttt ctcatggcct ggtattggcg caataattct
    15901 ccaaggtgta tccgcaagcg attatccagt cataatgatg accacattaa tgatcgcggc
    15961 ggcaatgctg cttgcaagtt ttctagtcga tctgcttatg gcgcttctcg atcccagaat
    16021 acgattcgag cgatagaagg ggttgccaat tatggatcaa cagagaataa cgaaatcaag
    16081 cctagctctc aggagctgga ataagcttgt tagaaataag cttgcgatga taggtaccat
    16141 tgtagcgata gcgatgatat tggcatcgat attcgcaaac ttcattactc cctatagtcc
    16201 ttctaagatc gattttacaa atatgacaaa gccaccgagc tggaaacatc tgatgggcac
    16261 cgataaaatg ggccgagact tgtttgctca ggttctctat ggaggtcgaa tttcgatcta
    16321 tgtgggcgtt gtaggcgcta tacttggctc tgcggtaggt atgattttgg gctgtattgc
    16381 aggttggttc gggggcaaga ttgacgcctt tcttgtaagg ctttcagaga tattcttgac
    16441 tttccctaac atgatattgg tgcttattct tgtggcattt gtggggcagg gtgtaaacaa
    16501 tcttatattt atttttttca taaccggttg gatgacgcca tttaggatga ttcgtaatga
    16561 gttcctttcg cttcgggaag agacttatgt ggaagtgtgc aaagccttcg gtatttcgga
    16621 tttatccatc atgttcaagc atattttgcc taatacgata agcccgataa tggttgcgac
    16681 tacaatcaat attgctaact atatccttca agaggcggga ttgagttttt tggggctggg
    16741 agttccgatc accgtaccca cttggggcaa tattatgaat gcagcgaggt cgattgaagt
    16801 aattcggaat tattggtgga tgtggttttt cccaggcctt gtgatttcga tttttgtatt
    16861 agccgtgaat ttcgcaggcg atggtctgcg cgacgtgctc gatcctaatc tgtagaaggt
    16921 atcctagaag atgcagccag gcagacttat tcttgaggtc aaaaacttgc agacaaactt
    16981 ccgcgttggc aacgaagata tatcgataat tagaggagtc tcattttctg cgcggcaggg
    17041 agaaacgctc gccatcgttg gggaatcggg ttgcggaaag agcgttacgg tccactctgt
    17101 gatgcgtctt cttccaaaga atggcagact gacaggtggt gaggctatat ttcatgatat
    17161 agacaataat tatgatttac ataacctaga accttttggt aaggagatgc gatcgatccg
    17221 cggtagaaag ataggcatga tttttcagga tcccatggca tctttaaatc ctgtatacag
    17281 agttggagat caggtggctg agaatcttct tcagcatttc aagatgagca aaaaagaagc
    17341 tcgcgagcgt atcgtagaga tgtttgcaag ccttggcata ccagacccgg ctcgaaggat
    17401 agatgattat ccacatcaat tttctggcgg aatgaagcag cgtgtgatga tagcaatcgc
    17461 catgatatgt aacccagatc tcctaatagc cgacgagcca accacggcct tagacgtcac
    17521 cattcaggct cagatcatag agctgatgga aggcctaaag gatgagcaag gcaaagcggt
    17581 cattttgatt actcacaata tgggtttggt tgcagagacg gcggatgaag tggccgtgat
    17641 gtatatgggc agaatagtcg aacgcgcgcc tgtagaacgc attttccata atccctgtca
    17701 cccttatacc aaggctctcc ttcgatcagt accagttttg ggccatggga aaaccgagag
    17761 gttggagacg atacgaggtt ccactccttc tccttccgaa tgcacaaaag gctgcgaatt
    17821 cgctaaccgc tgtgatttta tgatggacaa gtgtcgtgtt cttgatgttc cggtatatga
    17881 aatagaagag aatcatcacg tccgctgtat gcttttcgag gattcaaagg agttgcagca
    17941 atgaattcgc aatcatatca gcaactgcct gagaattgca gtcaaggaaa tcccggacaa
    18001 gaaggaatca tcttcgagat tagaaatctt aagaagtatt atccagtttt ggctggcttt
    18061 ttccgcaaga ccgtaggatg ggtaaaagct ctcgatgatg tgtccataac aatccatgcg
    18121 ggagagacgc ttggagtggt tggagaatcg ggctgcggca aaacaacatt cggtaagaca
    18181 atgatgatgc tgcagcatcc tacttcggga gagcttcttt ttcattttcc ggaaggtaag
    18241 cgagaccttg caaagctgag ctctaaggaa ctcatgccct tccgtagaaa agtacaaatg
    18301 atatttcagg atcctttttc agccctcaat ccctctaaaa aaatattcga ttcttttgat
    18361 gaaccattac gagttcatgg ccatgaggat cctaaagagc gcaaagaaac aatagtcaga
    18421 aaccttgaga tggtaaatct cagtgcagaa tatatgtatc gttatcctca tgaattttcg
    18481 ggtggacagc gacagcggat atgtatagcg agggctttgt gtataggccc ggaaatggtt
    18541 atctgcgatg aacctgtatc ggcactcgat gtatcgattc aagctcaagt tctcaatctg
    18601 atgaaagacc tacagaaaca gcttgggctg acctatgttt ttattgcgca tgacttaagc
    18661 gttgtccagt atatgagcga caagattatg gtcatgtatc ttgggaaaat cgtcgagatg
    18721 gcggattcca tttcgcttta tgagatgccg cggcatcctt atacgcaagc ccttctttct
    18781 gctgttccaa ttccttccat agatgtaaag aaaaagagga taatcttgca gggagatgtg
    18841 ccaagcccaa tcgataaacc ctctggttgc gccttccatc cgagatgtag agatgcaatg
    18901 gatatatgca ggagaataga gccagaattc gctcctcttt cggacgatcc atctcatttt
    18961 atagcctgtc atctatatgg aaaaacaaac ggagagtccg agccaacaaa gaagggcaaa
    19021 ctatgaacaa accaaaaaaa tatttattgt tcgatcgagg catcattgat gggcacctat
    19081 gccaaaatgc agtgctccgc ctggcaaaag cagaaaaaga tacgaaaaac aaccccatga
    19141 tgacagagga gttttttgcc aatccttcca agccctggga agtgcgctac gataatgggt
    19201 atcctaatgt ttttcgggat atagagcaga atgtctttag gctctattac actacatttt
    19261 cttatgacaa agactcggct cagaccagac tcgaagagcg gccttttagg caatatatgc
    19321 catccaaaag cagggcgacc tgcacttgct atgcaatgtc aacagatgga atacattggc
    19381 aaaaacccga gcttgggctt tctgaattcg aaggaaacct ggccaacaat ataatattga
    19441 agaatgctca tggtacgagc gttttgtgcg acagagccga tcccgatcct gccaagagat
    19501 acaaactcat cacaaaaatg cattattctc aagaaaacaa ctatatggca gttgcttttt
    19561 ctcgggatgg tatcgacttc agcactcctc agccatggcc aaaatacaat ccgccagcag
    19621 atacacataa ctttgcattt gtggatccaa agacaaaccg ttatgtgctc gtcacacgca
    19681 tttggaaaga tggcattagg atcgctgcca aatccgagag cagcgatttc ttgacatgga
    19741 ccgagcctga agaaatcgct cgcgggatag gcttcgatag ccaagtttat tcaatgccag
    19801 tttttttcta tgaaggattg tatcttggac tcgcttcgat ctatcacgat ggcgacagat
    19861 cagcaggaaa ttttgacacc gtagatctta gtcttatgta tactttcgat cttcgacact
    19921 ttgattggat tgaagctggc gctactctta tcgagagagg aaagggaaag tatccggatg
    19981 gtgattggga ttgcggatgc atatatgcaa gctctccaat caagatagac gacaggatat
    20041 gcatttacta catgggcgga aacggccagc ataccaattt cagagaaaca tcccttggta
    20101 gagcatggat agacaaggat aaatttgcgt attatgcggc aaaagaaagc tcaaaagaag
    20161 caactctcac attgaggcca gcaaatttct atggcgaaca tctcgagata cttgcagata
    20221 tagaagacga tgggtggata gactgcgagt ttatcgatgt aagcaattca agctacttcg
    20281 atgaagtggc tagtgccaga ggaagaaaac aagttacgcg cattactaaa agtggatgga
    20341 acaaaataat atttggcaat atgtcgacat catatttagc tgaaaaacct ttaagtcttt
    20401 gtcttaaatt caggaattcg agaatctacg caatagaagg tgatcttgcg atcacaagac
    20461 aagaaagatt ggagcgcgga tgaggagaaa tcgatgaaac tagaatcagg catctatgcg
    20521 gcacaactcg ttccgtatac atcagatggc attgtgaaag aaaaatctct ttcggcgatg
    20581 gtagagagaa acattacgct ggggaaagtg gatggccttt acataggcgg aagtacggga
    20641 gagtcttttt tgagcgacac caatgccaag aaaaaggttc tccaaattgc agcggagaca
    20701 aatgctggcc gggtttcgat gatagcgcag ataggatcga taaacgtcaa ggaagcagaa
    20761 gagctggcac atttggcagc ggcccttgga tatgatgctg tatctgcggt gacaccatat
    20821 tattatagat ttaatttttc agagatatgc gattactatt ttagactagc cgaagcggcc
    20881 aacctaccaa tgttagtcta ttatatacca gcatttacag gtacaagttt tgacatcgaa
    20941 caagctcgaa ggctctttga acatccattg atcgctggat tcaaatatac atctggcgat
    21001 ctctatacca tggaacgact tatctctgct tttccagata agatcgtatt ttctggctac
    21061 gatgagctat tgctttgcgg taaagcgctt ggagcctttg gtgcgatagg ttcgacatac
    21121 aacctgttcg cacatatagc caaagaaatc tggaaaaatg tggatcttgg tcttatttct
    21181 caagcacgcg ccggccaagc caaactcaac agcgccatcc aagagcttac aagccttggg
    21241 ctctatcagg ccctgaagga aataatatcc atatcgggca ttgacacagg atgctgcctg
    21301 ccaccatttt ctccgttatc gcaagagcaa aaagaaaaag ccgatgcgct tggtaggaaa
    21361 tatgtagata tgaaattgat cgggctttga gcaatagttg tattttgtcc ttatacttgg
    21421 caaggagttg tgcgccaaat ttattgtgta ctgaaagttt tattaatttc caggaagtag
    21481 aagtgccata tccttatcga ttattgcgca cacattatcg ccttcctgat atatatcaga
    21541 accaggccgg aagggctcga aagccaatag tttgcctctt gagcctaaat ctaattgata
    21601 ttcaatatga ccacccataa aaacagttga attcacaatg gcttgaatgt tttcagtttt
    21661 aggctcacaa gctatatcat ctattctttc tatttgtctt tttcttacac ggatattttc
    21721 tggacgcact atgagtgatg ctttgcttcc cgatctaata cgcgagggat ctattattct
    21781 tgctgtataa tgccagccat caggaattga aaccaaagca aaattgccct cgatgctctc
    21841 tactattact tctaatatat tagcttgtcc aataaaatca gcaacgaaaa gagatgaagg
    21901 ttgagcataa atatcgaagg gagttcctat ttgttcgaca tggcccttat tcattaccat
    21961 aattcgatct gccattgtaa gcgcttcaga ttggtcatgt gttacataaa ggcatgtaat
    22021 tccgagtttt ttctgaattc gccttaactc cgttctcata taaatgcgta gcttcgcatc
    22081 aagattagac agaggctcat ccattaacag tagctttggt tccataacta atacgcgtgc
    22141 aagtgcaact ctttgctgtt ctccgcctga aagctgatta ggaaagcggc cttcttcatt
    22201 tgtcagccct acaagatcga gcgcattttt tactttgact ttaattgtgg attctctttc
    22261 gtttctagat cttaatccat aagctacatt gttgtataca ttcatatgcg ggaatagagc
    22321 ataattctga aacacaaaac ctatgcctct cttgtttaca ggcacatttg tcatatccga
    22381 gccttcgatt aatatcttcc ccgatgaagg caactcgaat ccagctatca tgcgcaatgt
    22441 agttgttttg ccacagcctg aaggcccaag gaatactacc atctctccct gattaacaat
    22501 tagatttacc gaatctactg ctgttacttt ttctctttcc ttatggaatg tcttggtaac
    22561 attgatgagt tcaagcgccg ctgttgtcat agatattctc ctgtgcaata ccgaattttt
    22621 ttgtgcttaa tttcatcatg cctcccataa tcgcatcagc gataaagaca agcacaataa
    22681 gtacgatgga aaatgctgaa gcctgagaga actgtaattc tgtcatactt tctagaatgc
    22741 gggtcgttaa aagcgtccat cgtaccgaaa ccaagaatat ggttgcgctg atagctgtca
    22801 tacaatgaat gaacaaataa cgcatacctg ctaagactga aggtataatt agaggaatgg
    22861 ttaccttgaa aagagttctt actgatcctg caccaaggct tgccgatgct tcttcgatcg
    22921 acgggtctat ttgctgaagt gatgctacta ctgagcgaat gcctgtcgca taatagcgaa
    22981 atacatacgc agcaaccaaa atggtcattg ttcctgtcaa tagaatcggc ttttcgttga
    23041 aggccaatat ataagcaata ccgactacag ttcccgggag tgcatagttg agcatgctta
    23101 taaactctag agcgcgtttg ccagcaaatt tttttctttg aatgatataa ccaatgataa
    23161 tagcgaggaa tgagcctatt aatgtcgaaa taatggctat cttgagcgta tctaatatag
    23221 ctttcttgcc atttgtgaac acataagaaa aatgctcaag agtgaaagta ttatcgacgc
    23281 tccatacttt gacaagagaa ccttggagaa taataaaata caagtaaaca atgaacaggg
    23341 ctataaagct gcagattaca gctgttaatg tctcaagcca gaaaggaagt ctcgaaaatt
    23401 gggactgagc tccgcttttc ccggttatgg ttacatagct tttctttcca acaagatatc
    23461 tttgaaaaag aaaaacaaga agggcaggta agataagtaa gaagctgaga cttgaccctc
    23521 cttttaagtc atagagtccg gtgatttgaa gataggcttg tgtaggcagt atcgggaagt
    23581 tatgaccacc caaaataagt ggtgttgcaa agtcagcaag gctagaacca aataataata
    23641 aaattgaatt ggcaatacct ggcatcgata aaggtaacgt tattgtgcgg aatatccttc
    23701 ctctacttcc ttccaacgat aaagccgcat catcgatatt agcatccatt gtacttaata
    23761 ttgctgaaag taccatgaaa gcaactggga aataagttaa tgtctcagaa agaaatgtgc
    23821 cccaaaaccc gtaaaagtta agatcatcaa gatgtagagc ttttagcaga atcccatttg
    23881 gtcctagagt aagtgtcaga gaaatagaac ttgtaaacgg gggcgaaatc aatggaagta
    23941 tagttattga acttaaaagc catttgaaat acttaggcca attagttctt gttacagcta
    24001 gtgcaaaaag atatccaatt agagttcctg cagcactaac tgcagcagct agcaagatac
    24061 tattgcaaaa tgctttttgg ttgtatgttt ttcttaggat tttagtcaaa ttatctaaag
    24121 aaaaactgcc tttaactaag aaagttgttg caagcagttt tagcaaagga tagagaataa
    24181 atatggcgaa tgaaagccag ataataacaa gcgctagaaa gagagctgga tctttacgaa
    24241 ttatatgaac atgctccatt ttcccatttc tgtctttcgc catactaatt tccttttcat
    24301 caatagagac taagaaaaga ctaagaaaag cagagtcgtc taaacaaccc ttgctcagat
    24361 acgactctgc tgttgctaga aaaaatactc aaatttgtaa aataagatta ttgctcaatg
    24421 acctcattga cccatcgctc aacatatgcc tgccgatttt tgcctttcca tgcaatatca
    24481 gcatcaatga gtttgacttt tgacatatca agattaggat ttgttacctt tacatctggg
    24541 cgagtgggta tataattgat ttttttatcg accatgactt ctccaagacg tttagatgaa
    24601 gcccagtcca tcaatttttt tgctgcagta agattcttag cacctttcag aataccagca
    24661 gcttcaacac cataggtcac tccctctttt ggataagaga gcactacatc ataaccctgc
    24721 tgctgaatgt cgagggcatc gacaatatag aaaactccgg atgcagcctg gccagtcgcg
    24781 atgggcattg cgccaccagc accgctcttt gtatagagtt gtatattttg atttaatttt
    24841 ttctggtatg cgaaagcccc atcttcaccg tatatcttta ccaaagtata aatcctttcg
    24901 gttgcggttc cagaggttcg agcatccgcc atctggagag cattcttata tgctggattc
    24961 aacaaatctt cccagcttgt cggagggttg agatgatgct cttttaagaa tttcccatta
    25021 ctcataaata ctagtgggat aattcctatg cctatccaat aattttccga tgatttaaaa
    25081 ctagcaggaa tcttgtctgc ctcttttggt ttgtattgct cgaatattcc ttcaagtatg
    25141 cccgcttcat aggtatctgc agggccaccc caaaggcaat ctacttgagg attattcttt
    25201 tctgcaatga ttcttgcgag agcttcgcct gctgacaatc ttataaaatt aacttttaca
    25261 ccagtatcgg cggaaaaggc agcaaatacc tgagtagcat acttctctgg cattatcgta
    25321 tagacgttta gcgtttcttg tgcataaagc atagcaggca acacaagcaa aacgaacgct
    25381 aatcctagta aaaaccgttt catcaatact tcctcctcca aaatatatga gaaaatttat
    25441 aaagcattat atgtcttggt tgcaagcatt gtcaatgaag catgtttttc ttatcaaaag
    25501 ggacttttat aaaagtcatc taacaagcat aaatagttaa ggactactaa aaataggaaa
    25561 agtcgaatat accaccaaca ttcgatagcg atctattaat agggaaagcc tattagctaa
    25621 aataaagtag tcaggccgac aaagaaacag aaccatatag aaagaataga ttatgaacga
    25681 agttataaga gtgtttgtaa ctttttttac tctctataag cctccgaaaa cctatgcgat
    25741 aatcccataa gttgctcgac ttcgaggcgc atcgattcgg tgaagagccg tgcatccgcg
    25801 cggcgcgata tgagcttgat gggcaggccc tcacgctgca gtgcatacat ggcgttgatg
    25861 ggatatagtt gatattcgat gatggatgct agacctagaa aatcctgaag ctcgagtgct
    25921 cgatctggct ggtcgttcca gtgtttgcac atccatacat agaggtccga gtggaagtga
    25981 gtcataatgc ctgagtatcc tgatgctcct ttctgtagcg atgcgagcag agtcgcagca
    26041 tttgcattga aaagcttgaa gttgctgcct tttgccgctt cgattttggc tttcatatct
    26101 ccaatgcgac aacttgtatc cttcagaaaa ccaaatcgga cagtctcgac gatgaattgc
    26161 acgagatcgg catttagaag ccgtttataa ggagcagggc actcatagaa gccaaggagg
    26221 atatcgcggt cgaaggctgt caggaatgtc tcgagatttc tcttgaaaat atcgttacct
    26281 tcataggggc gcgccaggcg atttgtaact aggataagtg catcggcgcc cgtatcggtc
    26341 atcattttag cttcttcgat ttgatcgtat agcgtatcgg cgacatgccc ggaaacaagt
    26401 acaggtacgc ggtctcgcga gaatttgatc gacattcttg ccaattcaag ccgttcgcgc
    26461 agtgtcagat ggaacatctc gctcgattgg cagacggcaa agaatcctgc tactccgtgt
    26521 gcaatatgcc actcaatgag tgtttcgagc gcattccagt ctatgcgacc atttttttcg
    26581 tagggtgtta taagggtcgg ataaacacca tcgggaatag tcttattcat tgtggcctca
    26641 tttatacccc gataacttgg gaataaaaag cacaagatcg ggaatatagg ttattaagaa
    26701 caagacaaca atgagaacaa taaccatagg catgatttct cggattatat cttttagtgg
    26761 agtccctgta agccccgata ccgtaaagag cagcatacca tagggcggag tcgaaagtcc
    26821 aatcattaga ttgagcacca ttactacgcc aaaatgcacg aggtctattc ctaagtggct
    26881 aagaataggg attatgatag gggtgaatat gagctgtatg ggcgtggtat ctagaaagca
    26941 acccagaatt agcatggcga tgttgaggat gaggagcatg gcatatttgt tctgggttag
    27001 accgagtaag aatgtagcta taacattggg tatttgttcg atactgacga tataagaaaa
    27061 agcgaaggag cttccgacaa gaagcgaaag tgtcccagta gtgcgcaccg catttataag
    27121 gcactgcttg aattccttcc agccaagcgc tcggtataca aagaccgata ttagtattcc
    27181 atagaaagcc gccaatgccc ctgcttccgt gggggtaaca acgcctgtat agattcctcc
    27241 cagcaagatc actacggtga aaagcgcagg caaagcttgc aaagtaaatt tccaaaattg
    27301 cttggcagga aacttttttc cttccggata gttcctttta ttcgatataa atgtgttgta
    27361 aataaggagt gcgatgccaa cgagcagccc tgggataatt cctcctagaa agaggtaacc
    27421 tatcgacgta ctggagagca ttgcataaac gaccataggg atgctaggtg gaataattgg
    27481 cccaatagta gcgctcgctg cggtgatggc acaggtatat gcatcatcat agccctgttg
    27541 ttgcattgcc tttaattcca tattgcctgg acctgatgca tcggcaagtg ccgagccggt
    27601 cattccagaa aaaataatcg agttgataat gttgacatgt gcaagagcac cttttcgctt
    27661 gcctacgagt gcgctcgaaa aggagaagat catctcggtt actttgcctg agttcataat
    27721 ctcggccatg aagacaaaaa gtggcaccgc aatgagaacg aagctcgcat tgaggtttgt
    27781 aagaaactgc attgcgacca tatctagcga cgcagcagga tttttagaaa ggaggaaata
    27841 gaatgcgctc gatagcatca tgcctggtgc aagaggaata ccaagcagaa atacagccac
    27901 aaacgacaaa acgaaaacaa taagtgggag gttcatttgg tatctccaga gatgctaaaa
    27961 tcttttgcaa tgtcaatggt tagccttatt atcatatcga ctagatacac cacaaatgga
    28021 aaatacgcga tattcatggg aatgcccagc gcatccgact ttttatatcg cataaattgt
    28081 acataatccc atgaaggaac aagaatgatc aaaaaagcta caagaagcat agcattgcca
    28141 acaatgcgca taattcgctt tgtctttggt ttggcggcat catagagcat tgtgaaagcg
    28201 acctgagtgc ccattctctt cgagtaaaga ccaccaagta gagcagtcca tacaaagcaa
    28261 atcaaactca attcttctgg ccatagaagc ggcttgaaaa atttacgagc gaatatctgc
    28321 agcacgaaag agacgaaaag cgtgctaaac gtgagtatag gtacataaag ttcgatgcag
    28381 tcgaccagga atttgcctgt ttttttcagc caatacggca taatggctcc tccttcaaat
    28441 accctttcaa gaagcatcga tacgatatcg acatacttat tgaatcaagg ttgcccgcag
    28501 cggagatttg ttgcgggcaa accttaggta ttatttcatt ttttgtattt catcatagag
    28561 agcgaggtcc caatctttcg aaatatcttt accttccgtg agatagaaat tcttagcata
    28621 ggctgcaaat gcatcccgat cagggttttc tatgacaaca agaccttgct ggcggaagaa
    28681 atccaggatt tccttttcgt tatcgagatt tgtcttatcg caggattggc gtgctttttc
    28741 caatgcttgt aggacccagt cctgctgttg ctttgtcagg ctctgccatt ttttctcatt
    28801 gatagaaggc cataccgagt cgactagatg gtttgtgaga actatatact tggtgacttc
    28861 atagaatttg gcattcttat ctgttgggag agggttatct tgaccatcaa ccgcaccggt
    28921 tttaagaccc atatacaatt cattaaaggc cattggggta gggtttgctc cgagagcctt
    28981 gcccattgcg atccatgtcg ggctttgtgg aacgcgtaac ttcacacctt tcatttgttc
    29041 gggcttggtt acagggccga cttttgctat gaggttgagc tgacgagtgc caaggtaaaa
    29101 agcacagagt ggtcgaacac cggttttttt tgccacatct tcgaacattt tcttgccaat
    29161 tgggccattg aaggttttag tcatctgatc atagctctgg aatgtatagg cggcgccaaa
    29221 catcgaaaga taaggaacaa attctgcaag ccacgatgca gaactataga ccatatcgac
    29281 tgttcccttg cgaatggcat cctgctcagc ttgctgggta aagagcttgc cggaatcgta
    29341 gacatcgatc tggatttgtc cgccagaaag cttttcgact tcttccttga aaacgtacat
    29401 tgcttgtgta tgcgcatctg ttggcacact gactgaagta aacacaagtt tgattggctt
    29461 ttgttgggcg aacactgttg ggcttgcaat cataagcaat accagcattg caacaatggt
    29521 tttcttcata acgaacctcc ttgaatggat catctatttc tagccatacc gaaatacggc
    29581 taaatgcctc gctattatat atggcaatac aatatccttg cctttatcaa ttcaattggc
    29641 gatgttgaga tttaatctta tctttcttta aagagagcta gttgaatcat tgccaatcga
    29701 atattttcaa cctcctctgg agttccacac atgagtggtg cgcgagggta cccagcatcg
    29761 aatccataaa gtctaagcgc ttccttaaga agcggtgcgg cattggtcct tttattttga
    29821 cccatgctct tcaggaaagg ataaattttc tgctgctgct ctgctgctgc ttgtactttc
    29881 cctgctagaa aatcttcaat catagatctt attaggtgcc cgataatatg agatgatccc
    29941 gaaacaacgc cacctatgcg tttttcgcca ccttgggcat aagcttcgag gatcatagta
    30001 tcatcgccat tgtagatgac gaaattttca ggtgtggaat ttaaaaaggc tgtgatctgt
    30061 ttgggattca gttcggcttc ttcttttatt ccgactatat taggctgttt tgcaaggcgg
    30121 cttaccgtgg ttggttcgat attgacaccc gtatagatag gaatattgta taagagtatg
    30181 ttgagggata cagatgtagc tacctgcata aaatggcgat ataactcttc ttgatttggc
    30241 tttgtataat aaggagctac gatcatcacc gtttcgaaac cgattttttc agctattttt
    30301 gctagctgaa ttgtctcgat ggtagaggct gctccgacgc ctgctattat ggggattcga
    30361 tcgccaactt tgtctttgac tgtttcgaat aaccttaccc tttcatcaaa tgacatagca
    30421 taaaattcgc ctgtcgatcc cgaaagaaca agactatcac cttttttttg atcaattatg
    30481 aactcggcaa gcgcagcggt tctttcatag tctacgctcc catcttcgca gaagggtgta
    30541 agcagaggaa tcaatatcct tccatagcgt tccttaacaa attttgccat aaagacctcc
    30601 tcaaaactga ttgcactatc tagctgccta accggctaag atccttgttt cgatatccaa
    30661 ggcggtgtga tatctctgtt gttgcctcta agagggctgg agctatcgat tctaggcgtt
    30721 ttaacgtatt tctttcggca ggacccgata ggctgacaga tgcaaagacc ttagcttcca
    30781 cattgaaaat gggggctccg acacagcgca gatgatcctc atgttccatg tcgtcgattg
    30841 cgtagccttg ttcgcgaatg agttcaattt ctttccagag acgttcttcg gtcgttattg
    30901 tattagatgt aaatgacggc aagccctttc ttatgatgat acgccgtatc tcagaatctg
    30961 gcaaaaaggc tagaatagct ttgccaaccg atgtgcaatg gagtggcgcg cgaacaccga
    31021 taaccgaata ggttcgaagt ctttgatgcg actcaataca atctatatag agcacttcat
    31081 ctcgatcgag caaaacaagg tgtactgttt cgtcgaagcg ttgattgagg ccgcgcagga
    31141 aaggagccgc aattcgcacc agttcatgtc cagtttgtgt gcaataacct agttcaataa
    31201 gcctaatgcc gagtgaataa aagccagatt cacgattgcg ctccaatact ctttctgcag
    31261 taagtgtttc aagcaggttg gatgcggtgc tttttgcaag agatagctcg cgcgcaatgt
    31321 ctgatagact gcgcttttct ccatcggaaa gaagatcgag gattgccaga gcttttcgaa
    31381 cagaattgac ctgtgccatt caaatctctg taaacttgtt catatatagg aacagcgttc
    31441 ctttatatga attgtctcat gggaattgaa cgttgtcaag aaaaatttcc gtcaaaaata
    31501 gcttgtgggg gagctggcga tttatcgagc gctattgcgc ctatttatcc taaaaaattt
    31561 tatcgcggct tcgattagct tcgtatataa tgcggtatct ctgcgggaat ctcgatcgaa
    31621 agctcgacaa cgccagcaat acttccttgc tcacgccaag gcgcttggta gatgaatttt
    31681 ttaacacctt gtttttctat tgtataagtg tggggttcac cagtttcgag aatatgctcg
    31741 ataagacgca cggagctctg ttggtggcag gctcggacat cctgacctat aagagcctta
    31801 cctccccatt tagcattgga catggctgat ttttcgttca tatagagaat ctcataattg
    31861 cacccgatta ccgtaatgct agctccgaac tcatcctgcc aagcaggcat gattgcttcg
    31921 atatgtggta ttgtcatgca atgatcataa tttaaattgc caatctagcg aaagctctat
    31981 ggaggccata tttttgaaat tatcatagat tatctagaga ggtatgtctg taatgcagaa
    32041 agcaggcaaa aggcagacat agaaaggaaa agttgcaata tgataatgca gcaagttgtg
    32101 cattatcaat ttgtgcaaaa gcgatcagct tgcacgcaaa tttgtgtaaa aataatcatt
    32161 ttttcttgac agattataca tcatgaagga aaatgcttac atggctgaaa acgtttttag
    32221 aatttcagcc atcgaaggtt cttaggcatt taataggtct tcctgacatc tttgattcga
    32281 ttagatgctt atgatttatg gttgattaac atgatcctgt tttaggatca taaaatcata
    32341 gggcgagagg aggacattat gaaaagagta ctttcagtat tcatagctat gttgcttata
    32401 gccggaacaa taggtaccgt tggtgcacag gctcagaaac ggtataaatt tgtagttatt
    32461 acccatgcaa ctgctgttcc attttttgtt ccagttagaa aaggcgctga agatgcaggc
    32521 aagcttatcg gagcagatgt ggtatatacc ggcccaactg gttttgatat tcagcaacag
    32581 gttgaatttg taaaatcggc gattgcgcag aaggtcgatg gtattgccat gacaatgccc
    32641 gatgcaaatg cattcaacgc ggtagtcaaa gaggcgatgt caaaaggcat acctgttatt
    32701 gccataaatg cggacgctcc agaaagtgga aggcttgctt atattgggca aggaaactac
    32761 gaagctgggc gctctatggg aatgcagata gtaaaactgc taccaaatgg tggagatgtg
    32821 cttttgacaa cccatactgc aggtgcggag aaccttgaag ataggattcg cggcattaaa
    32881 gatgtactgg atgctcaagg aggaaaattc aaatatagag tggttgcaac gggtactgat
    32941 atggttcggg cagtcagcct tatttctagc gccttgcaag caaatccaaa tataaagggc
    33001 atgtttggag ttgaagaggt aacggggagt gcaatcgccc agattatcga gagagatcgc
    33061 ctgaagggca aagttatggc aggtggtttc gaccttgtca cagatgaact tgttgccatt
    33121 caaaaaggta atatgcagtt tactattgat caacaaccgt atctccaagg gttccagggt
    33181 gttatggagc tttatcttct taagaagttc aatcttactc catgcgatat caatactggt
    33241 attgcacctg tcacagccga aaacgtaaat gaagtcatga aacttgcggc tgaaggctat
    33301 cgctagaatt cgtttgttgt cataagaact agctctttca ttcggagacg gtgctttgaa
    33361 gtactgtctc cgcaccagac caattcatcg atgaggaagg agattgtatt gcagagttta
    33421 ttatctgtac aaaatatctc aaagcatttt ggcaatattc atgccctgag aaatgtatct
    33481 ttagatattt caccaaaaga aattatcggt ctggctggcg agaatggcgc aggcaaatcg
    33541 acactggtca ggataatctg cggtgctctt tcttatgatt caggagagat gagctatcaa
    33601 ggaaaaccat ataagccgca tgatgtctct aatgcagaat ctctagggat aagcgttttt
    33661 caccaggaaa ttccaatctg cccgcatctt tctattgcag caaatgtgtt tcttggccca
    33721 atgattccct ctcagagagg aaggcctgat tggaaattaa tgaataatcg ctgtatagaa
    33781 ttgtatagaa aattcctgga cgaagatata gatcccactg tcccaattgg agaatgctcg
    33841 gctgctgaac agcaattggc ccttctagta agagtgttgt ctcgaaatgc aaagttagtg
    33901 attctcgatg agccaacgac tgcattgtct gcaccggaag tagaaagact ttttgattca
    33961 ataagaagac taaaggatga gcaagggatc ggctttatat ttgtaagtca tctgcttgat
    34021 gaacttatgg aactatctga cgagattttt gttcttcgag atggtgaaaa tgtaggtcat
    34081 ctctacaaag ccgaattttc agcaaaggaa ttatcaaggc ttatcgcagg aagatcaatt
    34141 gatgagcgac atcgcattgc aaaggatatt tcggatgcag agattgctct cgaggcaaaa
    34201 tcgatttcat tatcgaaatc ttacgaagat atttcttttt cagtccataa aggtgaagtt
    34261 tttggtattg caggcctaca aggttcaggt agatctgaag tcatttcgag tctttttgga
    34321 ttgccaccac ctgataaagg ttcagtcgtc atccgaggta atgattttgg gaggataacc
    34381 atctctagag caatagccgc aggtgttggc tacttacccg aagacagaaa aaccaagggc
    34441 attttccccg acctgaatat agcaaagaat attggaattg tgagtatttc tcaatcaaaa
    34501 gatctaactg gctataatga ggaagattat ataaaggtag cgaataatat ggctaccaaa
    34561 ctttcaataa agatttctaa tcctagagat tatatatctg ctttgagcgg gggcaatcaa
    34621 caaaaagcaa tgcttgcgcg atggcttgcc ctatcgccat cgattctcgt actgaatgaa
    34681 ccaactcgag gtgtagatat tgcagctaag gctgagattg caaaaattat cttcgatatg
    34741 gcaggcgcgg gttgtacaat tattatggca tcatcagaga tggaagagct tttgatcatg
    34801 tgcgatagaa ttttggtgat gaatcgaggt cgagcaaaga ttattcttga aaaggaatct
    34861 ttgaccaagg agaatctttt cttttatgca atgagttaga tagaaaggat gaactaatga
    34921 acaaatcagc gaccaagaat caaaagaaca aatctagaat tgtattgggg cgcgatattg
    34981 gtgttcttat tgcagcaatc ttaattgcgg tattttttac gattgcttcg cctgtctttc
    35041 ttactgcata taattttttc aaccttttac ggcagactgc tcagcttggc ataattgcca
    35101 tggctatgac aatgctcatt atttctgggg aatttgatct ttcagtagga gcaatatatg
    35161 cggtaacagg agtcgttacc ggaatattag tcaaaaatat gggattcaat atttggccag
    35221 ctgcgctcat tggatttctg actgctcttt tgctgggctt cgttaatggt actctcattt
    35281 caacgacaaa gatcaattct tttattggaa ctctttccac tcaaatgata tttcgcggta
    35341 ttgcgatggt tctatcccaa ggtcagccaa tatcatttct ccagagttct attttctttg
    35401 atgcatttgg cagagctgaa ctttttggca atattccggt tcagatcttt tggctcttag
    35461 catggacatt gatttcgttc tatctcttac atagaacatc atttggtgta aaagtgtttg
    35521 ctacgggtga taatcggctt gccgctgatc tttctggaat aaagacaatg agcattaaga
    35581 agacatgctt catgattacc tctgctgctg caggcttgtc ttcccttatc tcacttgctt
    35641 ttctgaagac tgttacgcca actcaaggtt ccggtcttga actcgaggct atagctgcag
    35701 gagtcattgg aggaacttcg atgtctggcg gggtaggaag tattctgggt acttttatcg
    35761 gtacatttat catggctgaa gttagaacag gtctagtttt gatgggtact gatgcttatg
    35821 tacaagatgc ttttgttgga ttagttattg cattagcggt tattatcaat gttagattta
    35881 gcatttccaa ggatcgtaca taagcttcag gatgtgggtg cacgacaatt ttgtgcaaag
    35941 gtagctaagc agcatttttg atacttgaag aattattcga ttgagagtta atatcatcgt
    36001 ttgataatca tgaattttac cattcgcgct atcgctccag tcgaagcaga agattactat
    36061 tctcttgtca atgaagtaaa agatgaaggt aaatttctat ttgcgtccat tcgtattccg
    36121 ctcgaaaata tgagaaaata cattgctcag cactatgagt cttccaatcc agttctggga
    36181 gcatttgacg acaagaagga gcttctaggt tggattgaca taaacagaag caatatcgaa
    36241 gagcttgctc acatcggcac cattgccatg ggtgttagaa agagctttcg aggacaaggc
    36301 attggcggtg ctcttctcga tgcgtgtatc caccaagcgc gagaacttgg ccttgaaaag
    36361 ctggagcttg aagttttcgg ctcaaactcg gcagcccgcg ctctctataa gaagaaaggc
    36421 tttttcgaag aaggtattcg tctcaagaaa cgcaagttcg aagggcatta cgacgatttg
    36481 gtctgtatgg gtatgttt
//
LOCUS       DAOSOB010000012        33122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1365_length_33122_cov_63.613089, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000012 DAOSOB010000000
VERSION     DAOSOB010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 33122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33122
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1365_length_33122_cov_63.613089"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1075
                     /gene="buk"
                     /locus_tag="PLS88_02405"
     CDS             2..1075
                     /gene="buk"
                     /locus_tag="PLS88_02405"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015240178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOU06235.1"
                     /translation="MQSVVLVINPGSTSTKFAVFHGDRCIFDQTLNHSAEELSGFSTI
                     ASQYEFREKAIQEALKERDFDMSSLNAVVGRGGLLHPIPGGIYRINEAMKADLLAARY
                     GEHASNLGALIADTFAKQFGIPAFIVDPVVVDELEDVARVSGHKLFKRISIFHALNQK
                     AVARRFAKERGINYEDITLIVAHLGGGISVGLHRKGRVIDVNQALSGEGPFSPERSGT
                     LPAGDLARLCFSGQYTLQEVLKMITGKGGMVSFLGTNNMRDIEHMRDQGNKEADFYFQ
                     AFVYQVGKYIGSLAPVVEGKVDAIILTGGIAFWKEVTERISQMVSFIAPVVVYPGEGE
                     LEALAIAGQMALSGEAEVKEYVP"
     gene            1072..1998
                     /locus_tag="PLS88_02410"
     CDS             1072..1998
                     /locus_tag="PLS88_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOU06236.1"
                     /translation="MNHISDIIEEARKIGRRKFSVACAQEATVLEAVTEAYREGIGEP
                     ILVGNPRAIEEAAKEANEGKGADISPFSIIEENDMEKAAARAVALVRSGEAEFLMKGI
                     IDSSLYLRAALNKETGLNAGKIACHVAVMEVPTYHKLFAITDVALNIAPDLPTFIDMI
                     NSAVKVMKILGVNNPKVALLAAVEKVNPDKMPCTATAAILTQMNRRDQIKNCIIDGPL
                     ALDNAIDPESVRIKKIKSEVAGDADILVTPEIESGNVLYKCLLHLAGAKGAAVIMGAS
                     VPLVLTSRADDAETKLASIALASLLGGRGSQA"
     gene            2015..2230
                     /locus_tag="PLS88_02415"
     CDS             2015..2230
                     /locus_tag="PLS88_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU06237.1"
                     /translation="MAIKGSIEIDRERCKGCLLCIRACPTKVLGVDSEPNSWGYFPSR
                     VIAQEKCIACGNCYDVCPDVAITVYKL"
     gene            2242..3309
                     /locus_tag="PLS88_02420"
     CDS             2242..3309
                     /locus_tag="PLS88_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOU06238.1"
                     /translation="MAEEIRLMKGNEAIGEAAVRAGCRAYFGYPITPQNELTAYMARH
                     MLARGRTFIQAESEVAAINMVYGAASTGARAMTSSSSPGVSLKQEGISYLCGADLPAV
                     VINVARAGPGLGGISPAQGDYFQATRGGGHGDYYTIVLAPKGVQEAADLTYEAFGLAE
                     KWRMPVLILADGLIGQMMEGVVLPEPIDPVNLQRKSWAVGHSVEMARPYNHISSINLI
                     PDELEAMNRKRFERYAQIKKELIRYEEIEVEDADIVFVAYGTSARVSHGALQIARAKG
                     MKVGLFRPITLWPFPYEQLYRLAERGKRFLCVEMSMGQMIEDVRLAVSGKAPVYFYGR
                     CGGNIPSQEEVFAEALRLSGK"
     gene            3325..4098
                     /locus_tag="PLS88_02425"
     CDS             3325..4098
                     /locus_tag="PLS88_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU06239.1"
                     /translation="MEIAYKHPKSLKSIQTKYCPGCGHGVIHRIIAEVVDEMGLQTRA
                     IITNPVGCSIYADMYFDFDSVQPAHGRTPAASTGIKRMLPDHLVICYQGDGDLAAIGT
                     AEIIHAANRAEKFTTIFVNNAIYGMTGGQMAPTTLVGQHATTAPEGRDPINAGMGYPI
                     RVCELLASLEGTKYLARGAVNNMVNIRRTKKYIRKAFEAQMRGEGFTMIEILSQCPTN
                     WQMNPVESVEWLEKNMIPYYPLGEIKDTLSTAAAPAQAH"
     gene            4111..4647
                     /locus_tag="PLS88_02430"
     CDS             4111..4647
                     /locus_tag="PLS88_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU06240.1"
                     /translation="MTEKTFMTGFGGQGIISMGQLWVYCAMKEGLKVTFFPFYGAEKR
                     GGIARASVIVSDSEITSPIVTVADSVVIMNQDSVEIGEKVCKEGGIICVNSSLVKKDP
                     ARPDARIYHIPCNEIALKLGNVRVANMIMMGALSKVTGAVKLQDLDTILPSYFSSNKR
                     SLIEINIAAVEEGKKVVA"
     gene            4665..5666
                     /locus_tag="PLS88_02435"
     CDS             4665..5666
                     /locus_tag="PLS88_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOU06241.1"
                     /translation="MKPESQSSELPKGTKNRKARKLIWKVIRIALIAILIVHLVYIGL
                     TSALIFTYKFANPPTTVLMLYRSTVSHWKVQKPIPLKTSQIPLYVRRMLISVEDGKFY
                     EHHGIDIEAIKRARELNEKVGKPMYGGSTITMQVARTLFLLPTKSYVRKYFEAITALE
                     LELILSKDRILELYFGYAEWGKGIFGIERASRVYYGKGVASIDRDQAARLIALLSSPI
                     KYTPDTLYRSLILRQRYAYLVARYVAAVPVAAISVETPPPSGIEPSVDISEIPDAEED
                     VTAEQGISPNSEGLSEDTSSAPSSTETAEPALQVDTLKETTNQMKTDKSSTPGTSEF"
     gene            complement(5676..6236)
                     /locus_tag="PLS88_02440"
     CDS             complement(5676..6236)
                     /locus_tag="PLS88_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HOU06242.1"
                     /translation="MSAASQKDPLNSSSNSRANESDFTLTIWVDADSLPKDIRPMLIR
                     RAKTKRNYDGISILVRFVASRNLPEIPRDYLILVEPGDGAADFYIEAHAEQNDIAITR
                     DIPLAERLLAHSVHTMNDRGDIFTFENIGERRSLRDALAYMRDSGIAPPSPKGNLRKP
                     SYMKRFADALEKLIVQAVRQKAAPKK"
     gene            complement(6233..7486)
                     /gene="manA"
                     /locus_tag="PLS88_02445"
     CDS             complement(6233..7486)
                     /gene="manA"
                     /locus_tag="PLS88_02445"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HOU06243.1"
                     /translation="MDEKNNSIYTERRTPPCLSLLKNSAQQYAWGDTKGIIPFVEAEL
                     RPGYPIGEVWMGSHERAPSKIILDDSEFALDTLIKQDSAHWLGDHISKKYNELPFLFK
                     VLAAASPLSLQVHPNLEQAKIGFAKEESAGIGRLAPERTFKDPHHKPELAVALTSFQA
                     MAGFRSIYEICSLLGHSLCDSLEFKGKDKSELRSLIKRLFCVRGEEYLALETELHARA
                     EQLLHSEQKTARDAAHWVLELQSRYPGDPGQFAPLFLDLLQLEPGQGLFVPAGVIHSY
                     LKGSILEIMACSDNVIRAGLTVKHIDVDLLCNIVDLETKPLLILPEYQRFDWGTHAIY
                     KTPAKEFRLESIEVNSSASSSSITITSGGPKIFLCTKGSFRLHNGEQLELPARKSCFA
                     AGSCGQLELKGEGKLWIAAVGEIDT"
     gene            complement(7495..10053)
                     /locus_tag="PLS88_02450"
     CDS             complement(7495..10053)
                     /locus_tag="PLS88_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOU06244.1"
                     /translation="MSKKQSAILDVATRFSTEAQNELRSAILAMEGREIFAVGTLDSK
                     GLVKNIDILARGTESAVPAPFQKHSHAQVLIHNHPSGMLFPSDADIVVAAEAGAEGIG
                     SYIVDNEVEHVLVVAEPVKPKTIRPLDADEIAAVLDSSGKLSHIMPEFEPRLSQVEMA
                     HDVAEIISDGGILVAEAGTGVGKSFAYLIPALAWAIGNSERVVVSTATINLQQQIYKK
                     DFPLVSSLFKKQAKAVIVKGRGNYLCKRRLYEAIEEDALFSDSSIKLREILEWDNGGG
                     SGDKSDLALPDDDPIWSRVCSESDYCLSLHCPYHDKCHVIHVRLEAASAQLIIVNHHV
                     LLADLEAKRTREGSINTVLPSYQALVIDEAHALEASATSLFSETFSKRSIQRLLSRLS
                     RRKKRLQVGILASISKLGDIPSSLIDTARLQIEKAESSVDSFNAIACTCFSEKESSIL
                     VKNLSGINRTMFLSTLQNLEKEIALLVTRLGEISEAIALELEDEESVIELRITLRSLE
                     ETAALLARFINPEAEPSSIFWLQVDNKNPKEPMVICSATPLEVAPLLSERLFSKIRSC
                     ICTSATLTINGSFQWWRSRVGLLGTGISGDRSSALELPDKKLKHRQPSLTLEEDSTKN
                     LRVLQKNYPSPFPYSKNAMLAIDTEAPSPDSAEFQTYLNTAVAKLLKASRGRALVLFT
                     SHKALRDTYDAVSPLLEKEGILALRQGQKDRYSLLRTFVNDISSVLFATESFWEGVDA
                     PGETLSLVIITKLPFRVPTDPIQSSRAAAIDARGGNSFAEMSIPEAVILFKQGFGRLI
                     RHSQDRGVVAVLDVRILKKAYGSLFISSLPRCRLGMGALDSICRDVAEFLDNER"
     gene            10197..10499
                     /locus_tag="PLS88_02455"
     CDS             10197..10499
                     /locus_tag="PLS88_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06245.1"
                     /translation="MDSKTEQGIGLSLGNTHYVQPRWRSMPAEDIYRHSKNEEKDSYV
                     RALAKLQEEDEEILLPPERGPIVFRDGVFQIDMSRIEEKGDIDLELKKLIDNIMKP"
     gene            10612..11394
                     /locus_tag="PLS88_02460"
     CDS             10612..11394
                     /locus_tag="PLS88_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOU06246.1"
                     /translation="MIKSYSWNVNGLRACAEKGFLDWVETTAADFICIQEIKLQEPQL
                     TDELRHPMAYNGKLYHSFWAFSERKGYSGTGLYVLREPVSVTKLGISEFDIEGRTVIA
                     DYGGFLIMSAYFPNSQEAGMRLDYKLRFCDAILDFCNFERSKGKHVIVAGDYNIAHKP
                     IDLARPEQNEGNAGYLPEERAWMDKFTSIGYIDTFRLFCKEPGRYTWWSYRSPKAREK
                     NIGWRLDYHCVDEEFSPALLAADIHAEVMGSDHCPVSLFLDL"
     gene            11471..12025
                     /locus_tag="PLS88_02465"
     CDS             11471..12025
                     /locus_tag="PLS88_02465"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU06247.1"
                     /translation="MRVRYNAPVTLTFTLCAAIVLILSQTIMPAIIPNLFSTPTPFRT
                     NVFMDYVKLFTHVLGHANIQHFISNFTMILLLGPILESTYGSGFLLISILITALVTGL
                     SNAFLFPNVVLLGASGVVFMMIMLSSITNFSKGEVPLTFILIMIIYLGGQIWDALTKQ
                     DNISQFSHIIGGLVGSFLGFYHKK"
     gene            12056..13285
                     /locus_tag="PLS88_02470"
     CDS             12056..13285
                     /locus_tag="PLS88_02470"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOU06248.1"
                     /translation="MYGRIRDELEKDLEGLRTEGTYKDERIIVTPQGATIRVSDGAEV
                     LNFCANNYLGLANDPSIRKAAVRAMEEFGYGLSSVRFICGTQAQHKELERRIADFLGM
                     EDAILFSSCFDANGAVFEPLLDEESAIITDSLNHASIIDGVRLCKAKRWIYKHADMRD
                     IEEIDPETGKPLKGLERCLKEAQGSHRIMIATDGVFSMDGDIANLNAICDLAEKYGAL
                     VMVDDSHATGFMGTAGRGTWEHCGVGGRVDIITTTFGKALGGASGGVIASCKEIVEYM
                     RQKGRPYLFSNTLAPAIVGGTLQALELITRSSELRDRLDSNTKLFRSLMTKAGFDIRE
                     GEHPICPVMLYDEKLAHRMADELLSEGIYVIGFSFPVVPKGKARIRVQISAAHTQEQI
                     IEAVEAFKKVGKKLRVI"
     gene            13422..13727
                     /locus_tag="PLS88_02475"
     CDS             13422..13727
                     /locus_tag="PLS88_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU06249.1"
                     /translation="MPTYEYECRKCGYTFEAFQSINDDPLKTCPLCGGAVKRLIFGGS
                     GIIFKGPGFYITDSKKSQSAATGSEKSSSSSVNSSHSESSKEGTKETPKTDPSEKAS"
     gene            complement(13757..14626)
                     /locus_tag="PLS88_02480"
     CDS             complement(13757..14626)
                     /locus_tag="PLS88_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06250.1"
                     /translation="MLFRDRCDGIRITGQHALNALMPYMMRGRNESAVYYEKEINIEN
                     ALAYLKTINTSQTSKKDQDRFTLFGLIMTVALRTISLYPQLNRFIHRRALYQRKHICF
                     SYIVKQEFTIDAPEVNAKIYLEPEDSFSSINARMSDAILEARIHGEGEGERFAGIFHK
                     IPGGKAVLMTIYRLLDSINLAPWSLLRMDPLYTSIYFANLGSLGLDAPFHHLYEWGTA
                     SIFVAIGKAFSKELWHGGTRTRQRYIDIKITLDERIADGFIFSQAASTFYRLISRPEL
                     LDLSLEDLRKALA"
     gene            complement(14728..15438)
                     /locus_tag="PLS88_02485"
     CDS             complement(14728..15438)
                     /locus_tag="PLS88_02485"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOU06251.1"
                     /translation="MKNGTIGILGGMGPEATAAFFSMLVRLDSATSDQGHLHIIVDSD
                     PSIPDRTKFLLEGGTNPLPAMLSSARRLLAAGADIAGMPCMTAHALLDRIRHNCTLHI
                     LSAFEETSIALKEFSPPIRNLGILATMGTRQAMLFETAFPDKDIIWPDEALHRSLVVE
                     AIYGSKGIKAGNLGNEPKHLLLEAAHILQNQGAEAIVAGCTEVPLVLSQKDIEIPFID
                     PMYYLARALIATARKGLF"
     gene            15582..16889
                     /locus_tag="PLS88_02490"
     CDS             15582..16889
                     /locus_tag="PLS88_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOU06252.1"
                     /translation="MAEQKKKGALDWYFKSNLLTRILIGLVLGAIVGIILGFFPSSVK
                     PYVDNTKFFGDLFIRLLKMIIMPVILFSLIAGAASIAPSKLGRVGVKIMVYYLLTSAF
                     AVIVGLISAYIFKPGIGFNIVGEASVKGVQAAPPTIIQILLNIVPTNPFESLMKGDVL
                     PIIFFAVVFGIGISYIKDSKDQSLAKSGETLLNVVNAAAETMYKIVGGIMQYAPIGVF
                     FLISHVFAQQGAKAVGPLLMVTLSEYVGLAFHVLLVYGGLLTLYKLSFKKFLKGANEA
                     MVTAFVTRSSNGTLPVTMRCGEENLGIPRSISAFTLPLGATINMDGTAIYLGVCAMFI
                     GYATNQPLSFNQMLTVVITGTLASIGTAGVPGAGAIMLLMVLESIGLKVEAGSAVAAA
                     YAMILGIDALLDMGRTCLNVTGDLVGTSIVCKTEKELDLSKWK"
     gene            complement(17087..18073)
                     /locus_tag="PLS88_02495"
     CDS             complement(17087..18073)
                     /locus_tag="PLS88_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU06253.1"
                     /translation="MLQNEMTSPTLQTSEKTQPRQKTKKPPHFLCVCLNPTIQKTIVF
                     DKIEPGEVNRSSNWRIDAAGKGVIPTRILTQLGEKAIHLTQLGGPTRAWFLTMCAADG
                     ISVEWVESGAPIRFCTTLIEETEGRTTELVEESLQVAPETSDSLVERFVDLAKQADAV
                     LLSGTVAAGIEKGTMARFARIAWDVGAHLYLDIKKQDLLECLEFHPVCAKPNLEELAQ
                     TLGIPYANVRDEEFARKLVEEQGRRFFGEYGTYLVVTRGAKSTLYWDGQKLCEYPVES
                     VKVRNPIGSGDAFGAGLARILERHGSIHEAVAEGTRLGGLNAAQLKPGTLFS"
     gene            complement(18213..19517)
                     /locus_tag="PLS88_02500"
     CDS             complement(18213..19517)
                     /locus_tag="PLS88_02500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOU06254.1"
                     /translation="MAIEILMPQLGLTMNEGTITEWVKKEGETVSKGDIVFYVENDKA
                     VVPYEAQREGILGKILIQTGQTVPVGTPVGILAEKGEALEDIASLHIIKESIKEGIAN
                     EDNSKTQVVPDASAASASAEIESKAKLEIQKTIAQQASAIEPAGELGGLMAETFIPSS
                     PYARTIAKEKAIDLAILKGSGPQGAIVAQDVIAASASSKAFETEPITQKYEDVKLSRI
                     RQIAAERLTQSWTEIPQFTLSIEANAQGLIDAQSAFKSKEEKVSITVLLAKLMASALS
                     DYPLLNASWLGNGEIRVYKHFNISIAIDSPDGLVVPTLRDCGNRGIRDLGQELVNLAE
                     KARKRSLSPDEMQDGTITLSNLGMFGITRFRAIINPPQCAIISVGAITKRPAEGGHEG
                     ISFVPVIEIGLTADHRIVDGAYGARFLKRFRDLIEQPVLALG"
     gene            complement(19521..20501)
                     /locus_tag="PLS88_02505"
     CDS             complement(19521..20501)
                     /locus_tag="PLS88_02505"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOU06255.1"
                     /translation="MRTISFSEATLEAMREEMRRDPTVFVYGEDIVRQGGIFGQFKGL
                     PDEFGDRIRDTPISETAIVGAALGAALTGTRPVADMHFADFIGVAFDELANQIAKNRY
                     MFGGQANIPLVIRAPDGINNQAAAQHSQSVEGWIQNIPGLIIVTPSSPATAKGLLKSA
                     IRQNDPVIYFEHKVLYPQKGPVPDGEYLTPLGKAEIRRTGKDVTIVAYSIMVQKCEEV
                     AKQLEAEGIDVEIIDLLTLKPFDRKTIVESVKKTHRCVIAHEAVVIGGLGAEIAATIQ
                     NEAFYWLDAPIERVGAKNIPVPFSPVLEKYMAAQKEDIIQAIYRVLHKEG"
     gene            complement(20498..21529)
                     /locus_tag="PLS88_02510"
     CDS             complement(20498..21529)
                     /locus_tag="PLS88_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOU06256.1"
                     /translation="MSETKNPFPNRFDSLLVEEKMKMLETMIRIRTFETHVEELFMNK
                     KIFGFVHLYIGEEAIATGVMANLRTDDYITSTHRGHGHAIAKGANTSRMMAELFGRKT
                     GYCKGKGGSMHIADFSIGMLGANGVVGGGFNIAVGAALSSQIKKTDQVAVCFFGDGAS
                     NRGTFHEALNLASIWKLPVLFVNENNQYASTTPVSYAVSAQHVADRAKGYGIEGYTVD
                     GNNVAEVYDAANYLVGRAREGKGPALLECLTYRVKGHFVGDPERYRTREEVKKMVDER
                     DPIKLYKNRLLDEGLANEVGISKLYDQAEQEIQAAIEFAEASPFPDPQEALDDLFSFS
                     PKEFPEALQ"
     gene            complement(21547..>22488)
                     /locus_tag="PLS88_02515"
     CDS             complement(21547..>22488)
                     /locus_tag="PLS88_02515"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOU06257.1"
                     /translation="GLVFITPMIKQGYNKYFAATTIAAGAGLSIIIPPSISFIVYGQL
                     TGVSVGALFLGGIIPGVLVGIFLIGVVYIISRKNNYRGSEETGSGKQILKAFVNSLWA
                     LFAPVIILGSIYLGIATPTEAAIMGVFYALFVGIFVYHTLKIDVIFQALVDTVVSSAV
                     VMFVVAMAGVFSWAAATLGVIDAAANLISSLTTNQLVFMFLTMIVMLIAGMFLDAISI
                     TNVFMPMIMPALLALHIDPLYFGVCLTVSLAIGQITPPVAVNLYVTANLIHSNINNEM
                     VKFVIPMVIGSVIALILIVLIPDLSLAIPVLSKLYIP"
     assembly_gap    22489..22588
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22589..22994)
                     /locus_tag="PLS88_02520"
     CDS             complement(<22589..22994)
                     /locus_tag="PLS88_02520"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOU06258.1"
                     /translation="MAALLVFGIFILLIVIGVPIGTSLGTAAIGAILNYDLGMSMISR
                     NFEANIAKFPLVAIPFFILAGVLMDKSKIVDGIANFLILLTGKKRGGLAIAAVVACIF
                     WGAVSGSGPATAAALGLVFITPMIKQGYNKYFA"
     gene            complement(22994..23509)
                     /locus_tag="PLS88_02525"
     CDS             complement(22994..23509)
                     /locus_tag="PLS88_02525"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOU06259.1"
                     /translation="MKKNRIRIDELVSAILLAVMAIIAFVNVINRYFFHKGFAFTEEI
                     TVNLFVWITLLGIAIAFRRGANLKMTNLFDMFSPNLKRAALIISASLGVMIFCFLIYN
                     SAREIYKNITFYHAFSESLGIPTWIYSLGTPIFSLFVIKEIIASTIKEIKALNTSPPP
                     ADSVNSKKEAH"
     gene            complement(23603..24655)
                     /locus_tag="PLS88_02530"
     CDS             complement(23603..24655)
                     /locus_tag="PLS88_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOU06260.1"
                     /translation="MIYRKLLLSVVALLVAILPVTAFTPKKEYTMQVNVTQVVDWGAG
                     AQKFADLVAQKTNGVINIKPYWSSSLLAGKQTNWYQAVADGAIDFATESTMNACNVIR
                     PFNLFSLPFFINTYDNVDKIENGKSGARIIAEMEKGGVKFLAWGENGFRQITNSKREI
                     RTPKDVEGLKIRVVGSPIMIDIFKQLGADPINMNWGDAVTAFQQGVVDGQENPKGVLI
                     GNKIWEYHKYMTIWNYLIDPLVFCVNSQVWKSFPPDIQAQIQEAALEAGEWEKALARC
                     YLDGDKSLKIFKEKFKDPRDPENLFVVAPAKGMKITVLTAEEIAAFKEKTKPVYDKWV
                     PTIGEDIYKLALEDMK"
     gene            24954..26231
                     /locus_tag="PLS88_02535"
     CDS             24954..26231
                     /locus_tag="PLS88_02535"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOU06261.1"
                     /translation="MKKKIVLAYSGGLDTTVIVPWLKEHYDCEVIAVCGDVGQEADFD
                     AIGARALASGASKFIKLDQKEEFVKEYLWSLVKAGTPYEKKYLLGTSAARPLLAKGLT
                     ETALAEGADAIAHGCTGKGNDQVRFELGVKAFAPEMEVIAPWRIWDIQSREEEIAYLE
                     AHGIPVPMKKSDSYSRDDNLWHISHEGLDLEDPSNEPHFEGMLKMCVTPEKAPDKPEY
                     VTLAFEHGVPVSVNGEKLDPVSIVHKLNKIGGSNGIGIADMVENRVVGMKSRGVYETP
                     GGTIIMEAHDRLEQLCLDKKTLSFKMTVAQRFAEILYEGEWFSPLCTALRAFIDSTQD
                     VVTGQVTLKLYKGNMVFAGSKSPYSLYDASLASFTTGPLFSHNDSTGFINLFGLPTKV
                     RARLNERIKKYGIAASPDVVKPGNSGYTAHAGD"
     gene            26238..27629
                     /gene="argH"
                     /locus_tag="PLS88_02540"
     CDS             26238..27629
                     /gene="argH"
                     /locus_tag="PLS88_02540"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOU06262.1"
                     /translation="MGKLWQTSKGKELDPIIEQFLSSIESDKRIVFEDIECSQAHAIM
                     LGEQGIIPLQDASLLVDELAKIRKELEAGSLAIDLCAEDVHSFLEEELTRRLGDVGKA
                     IHAGRSRNDQVAVAFKLHMRNACDMCEKLVKKAIGVCLDLAEQHIDTLMPGYTHLQRA
                     QPVSFAHHLLAWCAALERDALRFRDAGERADESPLGAAALAGSGLPIDRARTASLIGF
                     SKTSANTMDAVADRDAAVDYAACSAILMMHLSRFCEDMSLWASSEYGFIAISPRSSTG
                     SSIMPQKRNPDAAELIRGKAGRVFGDLQALLVMEKGLPYAYNRDLQEDKSLFFDIEDT
                     VQSSLEAFAILVQCIEPNIERMRSALEKGYIEATDVAEFLVIKGIPFRTAYQAAKRLV
                     EHCIEEKKSLRSIAQADLSVHEAFDKAAIDARELAEYLDPVACVSRRSQIGGPAPERV
                     AEQIERLRRFIAP"
     gene            complement(27959..29158)
                     /locus_tag="PLS88_02545"
     CDS             complement(27959..29158)
                     /locus_tag="PLS88_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative CRISPR-associated protein"
                     /protein_id="HOU06263.1"
                     /translation="MDKTLICTVGTSFFNVNLEKITDQFDPLYVAYSKKDWKGLAQEM
                     LKTDPHERMLGAEINTIELILESIEKKGDRLESIIFLVSDTQDGANTGLVLQEYFDQR
                     AMRDERLAILKVKHWTVEDLQPQDPQLFKRSGLINLVRCIAKAIKEAGGATKVFIDAT
                     GGYKAQIAIAVMFGQALGVDVIYKHESFGNIIDFPPMPVSLDFHYFELYNDLFNRCYL
                     SAFETIPFEDLAQYFGDNILDYNDLQKNADFSNIKLFFEAEQINGKYFFSINHAGLIY
                     IEAAQQRGMQFVATFIPPEPSPKRTPPKFTDDHFPKGFKEYSDKIWSSEPWIDSIRSL
                     PYDRQRGIAERGFYVERRDGIPMIVGTYRDTKHFGARFNIQCDRLDNIAKIYAATMYL
                     NSKKDEY"
     repeat_region   30166..30874
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=30240..30274
                     /rpt_unit_seq="tcttaatcccttctatcaggtctaaatctcaaaac"
     gene            30979..32233
                     /locus_tag="PLS88_02550"
                     /pseudo
     CDS             30979..32233
                     /locus_tag="PLS88_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043257.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     repeat_region   32322..33103
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=32322..32357
                     /rpt_unit_seq="gtcttaatcccttctatcaggtctaaatctcaaaac"
BASE COUNT         8901 a         7282 c         7798 g         9041 t
ORIGIN      
        1 catgcaatcc gtcgttctcg tcataaaccc tggatctact agcacgaaat ttgcggtttt
       61 tcacggagac agatgtattt tcgatcagac actgaatcat tcggctgaag aactctcagg
      121 tttttctact atcgcatccc agtatgaatt tagagagaaa gcgattcaag aagcactcaa
      181 ggagagagat ttcgacatgt catcgctcaa tgcggtagtt ggacggggag ggcttctgca
      241 tccaattcct ggtggaatat atagaataaa cgaagccatg aaggctgatt tgctcgctgc
      301 aaggtatgga gagcatgcat cgaacctggg tgctctcata gcggatactt tcgcaaaaca
      361 gtttggaatt cccgccttta ttgtggatcc tgtagttgtc gacgagctcg aggatgtagc
      421 gcgcgtatcg ggtcacaagc tctttaaacg aattagcatt tttcatgcat taaatcagaa
      481 ggcggtggct cgtagatttg caaaagagcg cggtattaac tatgaagaca taacgcttat
      541 cgtagctcat ctggggggag gtatttctgt gggcttgcac agaaaaggca gagttatcga
      601 tgtaaatcaa gcgttaagcg gagaagggcc attttcgcca gagcgttctg gcaccttgcc
      661 tgcaggagac ctggcaagac tatgcttttc tggacaatat actctccaag aagtacttaa
      721 gatgataact ggaaagggtg gtatggtaag ttttctcgga accaacaata tgcgcgatat
      781 cgagcatatg cgcgaccaag gaaataaaga agcggacttc tatttccagg cttttgtata
      841 tcaagtaggc aaatatattg gttctcttgc accggtagtc gaaggaaagg tcgatgcaat
      901 tatattgact gggggtatcg cattctggaa agaggtcacc gagagaataa gccagatggt
      961 ttcgtttatt gcaccagtag tggtttaccc gggagaaggt gaacttgagg ccttggcaat
     1021 agcgggccaa atggcgctct ctggagaagc cgaagtaaag gagtatgtgc catgaaccat
     1081 atatcagata taatcgagga ggctcggaag atcggcaggc gaaaattttc ggttgcttgt
     1141 gcgcaggaag caactgtgct cgaagccgta acagaagcat accgcgaagg aataggggag
     1201 cctattcttg tgggtaatcc tagagctatc gaagaagctg ccaaagaggc caatgagggg
     1261 aaaggggcgg atatttcgcc attctcgatc attgaagaaa atgacatgga gaaagcagca
     1321 gccagagcag ttgctcttgt tcgctctgga gaagcggaat ttctaatgaa aggtattatc
     1381 gactccagcc tctatcttag agctgctctc aacaaagaga cgggcctcaa tgctggcaag
     1441 attgcatgcc atgtggcggt catggaagta ccgacctacc acaaactctt tgctattact
     1501 gatgtagccc tcaatatcgc acctgatctt ccaactttta tcgacatgat caattcagca
     1561 gtaaaagtaa tgaaaattct aggagtaaat aatccaaagg ttgcattgct ggcagctgtt
     1621 gaaaaagtca accctgacaa aatgccttgt actgcaacag ctgcaatcct tacacagatg
     1681 aaccgtcgtg accagatcaa aaattgtatt atcgatggtc ctctggcatt ggacaacgca
     1741 atagatcccg aaagtgtccg tatcaaaaaa ataaaatcag aagttgcggg cgatgcagat
     1801 attcttgtca cgccagagat agaatcagga aatgtcctct ataaatgcct attgcacctc
     1861 gcaggggcta aaggagctgc cgtgataatg ggtgcttccg tacctctagt tctcacgagc
     1921 cgtgccgatg atgcagagac caaactggcc tcgattgcct tggcttctct tttgggaggc
     1981 agaggttctc aggcctaaat ataaggagta actaatggcg ataaaaggat ctatagagat
     2041 cgacagggag cgatgcaaag gttgcctcct ttgcatccgc gcttgtccga caaaagtcct
     2101 aggtgtcgat agcgagccga actcctgggg atatttccct tccagggtga tcgcgcaaga
     2161 aaagtgcata gcatgtggaa actgctatga tgtctgcccc gatgtggcaa ttaccgtgta
     2221 caaactctga gggagaaaga tatggctgaa gaaattcgcc ttatgaaagg caatgaagcg
     2281 ataggcgaag cggcggtgcg agcggggtgc cgcgcttatt ttggttatcc cataaccccg
     2341 cagaacgaac ttaccgccta tatggctagg cacatgctgg caagaggccg aacgtttatt
     2401 caggctgagt ccgaagttgc agcgatcaac atggtatacg gtgctgcttc gaccggcgct
     2461 cgtgcaatga cgagctcttc gagcccaggt gtttcgctca aacaagaagg catttcttat
     2521 ctctgcggtg ctgaccttcc ggctgtggta ataaatgtgg cgcgtgcggg tcctggtcta
     2581 ggcggtatat cgccagctca aggcgattat ttccaagcaa cgcgaggtgg tgggcatggg
     2641 gattattata cgatagtcct cgctcccaag ggtgtgcagg aagctgcgga tctcacttac
     2701 gaggctttcg ggctcgccga gaaatggcgc atgccagtgc ttatcctcgc ggatgggcta
     2761 attggtcaga tgatggaggg ggtagtgctc cctgagccta tagatccagt caatctgcag
     2821 cgcaaatctt gggctgttgg gcactctgtg gagatggcaa ggccttataa tcatatatca
     2881 agcataaatc ttatacccga cgagctcgaa gccatgaata gaaagcgatt cgaacgctat
     2941 gcacagataa aaaaggaact aattcgttat gaggaaattg aagtcgagga tgccgatata
     3001 gtctttgtgg cttatggaac ttcggcgcgc gtttcccatg gggctttaca aatagctcgg
     3061 gctaagggga tgaaggttgg ccttttccga ccaattacac tctggccatt cccttatgag
     3121 cagctttata gactagcaga gcgaggcaaa cgtttccttt gtgttgaaat gagcatgggg
     3181 cagatgatcg aggacgtgcg gctcgcagtc agcggaaaag ctcctgtgta cttctatggg
     3241 cgctgtggcg ggaatatacc cagtcaggaa gaagttttcg ccgaagcgct aagactttcg
     3301 ggcaagtgag gggaggaatt tattatggaa attgcttata agcatccaaa gagcctcaag
     3361 tcgatacaga caaaatactg ccctggctgt ggtcatgggg taatccatag aatcatcgcc
     3421 gaggtcgtag atgaaatggg attgcagact agggcgatta taacaaatcc ggttggctgt
     3481 tctatttatg ccgatatgta cttcgatttc gactcggttc agcccgcaca tggtcgaaca
     3541 cctgccgctt cgacgggcat aaagcgaatg ttgcctgatc atcttgtcat ctgctatcaa
     3601 ggcgatggag accttgcggc cattggtaca gccgaaataa tccatgcggc taaccgtgcc
     3661 gagaaattca cgacaatctt cgtaaacaac gcgatctatg gtatgacagg agggcagatg
     3721 gctccgacaa cccttgttgg tcaacatgct acaacggcac cagaaggccg cgatcctata
     3781 aatgccggta tgggctatcc cattcgggta tgcgaactct tagcttcgct cgaagggaca
     3841 aaataccttg ctcgcggcgc cgtgaacaat atggtgaaca ttagaagaac aaaaaagtac
     3901 attcgcaagg ccttcgaagc tcaaatgcgt ggcgaaggct ttacgatgat tgaaattctc
     3961 tcacagtgtc caacaaactg gcagatgaat cccgtggagt ctgtcgaatg gctcgagaaa
     4021 aatatgattc cctactatcc cctaggcgaa atcaaagata ctctcagcac ggctgcagcc
     4081 cctgcgcagg cgcattgaaa ggagcgttcc atgaccgaaa aaacatttat gaccggcttc
     4141 ggtggccagg gcattatttc gatggggcag ctttgggtgt attgcgcaat gaaagagggc
     4201 ctcaaggtaa cctttttccc cttctatgga gcagagaagc gcggcggtat tgcacgcgca
     4261 tcggtaatag tttcggacag cgaaataaca agtccaattg tcaccgttgc cgattcagtc
     4321 gtcataatga atcaggactc cgtggaaatt ggagagaagg tttgtaaaga aggcggaatc
     4381 atatgtgtca attccagcct cgtcaagaaa gatcctgcgc gccctgatgc aagaatctat
     4441 catattccat gcaacgagat agcgctcaag ctcggcaatg tccgtgttgc aaacatgata
     4501 atgatgggag cccttagtaa ggtgacagga gccgttaagc ttcaagacct cgacactata
     4561 ttgccaagct atttctcttc gaataagcgt tcgctcatcg agatcaatat tgctgcagtt
     4621 gaggaaggaa agaaggtagt agcgtgatat aatgctctca ccttatgaag cctgaatcgc
     4681 agtcgtcaga attaccgaag ggaacgaaaa accgcaaagc tcgaaagctg atttggaaag
     4741 ttatccgaat agccttaata gcaatactca ttgtacacct agtctatata gggctgacga
     4801 gcgccctcat atttacatat aagttcgcta atcctcctac aacagtgctt atgctatatc
     4861 gttcgactgt aagtcattgg aaagtgcaaa agcccattcc cctaaagact tcacagatcc
     4921 ctttgtatgt taggaggatg ctaatctcgg tagaagatgg caaattctac gaacatcacg
     4981 gcattgacat agaggccatc aaaagagcac gagagctcaa tgaaaaagtg ggcaaaccga
     5041 tgtatggtgg aagcacgatt accatgcagg tcgcgcgcac actttttctt ttacctacta
     5101 agagttatgt ccgcaagtat ttcgaagcca taacggcgct ggagctagaa ctcattctct
     5161 ccaaagatcg tattctcgaa ctctattttg gttatgcaga atggggcaaa gggatctttg
     5221 gaatagagcg tgcttctcga gtctattatg gcaaaggtgt ggcaagtatc gatcgagatc
     5281 aggcagctcg tcttatagct cttctttctt ctcccataaa atatacaccc gataccctct
     5341 atcgctcgtt gatcttgagg cagagatatg cctatcttgt tgctcgatat gtggctgccg
     5401 tacctgtagc ggctatctcg gtcgagacac cgccgccatc cggaatagaa ccctctgtcg
     5461 acatatctga gatacccgat gctgaggaag atgttacagc agaacagggc atctctccga
     5521 attctgaagg attatccgag gatacatctt cagcgccgtc ctcaacagag actgcggaac
     5581 ccgctctaca ggttgatact ttgaaagaga ctacgaatca aatgaaaaca gataaatcat
     5641 cgactccagg gacttctgag ttttaaagca ttagcctatt tttttggtgc agctttttgt
     5701 ctcacagctt ggactatgag cttttcgagg gcatccgcaa agcgcttcat ataggagggt
     5761 tttcttaaat tgcctttggg actaggagga gcaattcccg aatctcgcat atatgcaaga
     5821 gcatctcgaa gagagcgtcg ctcgcctata ttttcaaaag taaaaatatc accgcgatcg
     5881 ttcatggtat gaacagaatg cgccagcagg cgctctgcaa gaggaatgtc tcgggttatg
     5941 gctatatcat tttgttctgc atgagcttca atgtagaaat cggcggctcc atctccaggc
     6001 tcgacaagga tgagataatc tctaggaatt tctggaagat ttcggcttgc cacaaagcgc
     6061 accaagattg aaataccatc gtaatttctc tttgttttag cccttcttat tagcattggc
     6121 ctgatatctt ttgggagaga atcggcatcc acccatatag tcagtgtgaa atcagactca
     6181 ttagccctag agttggagga cgagttcaag ggatcttttt gcgaagctgc actcatgtgt
     6241 ctatctcgcc aacagcagca atccaaagtt tgccttctcc ttttagttcg agctgtccgc
     6301 aggatccagc ggcaaagcat gatttgcgag ctggcaattc tagttgttct ccattatgaa
     6361 gtcgaaagct tcctttggta catagaaaga tctttggtcc cccagatgta atggttatcg
     6421 aagaagagga ggcgctggag ttgacctcga tggattcgag tcgaaattct ttagctgggg
     6481 tcttgtagat ggcatgggtt ccccaatcga atcgttgata ttcgggtaga atcaagagag
     6541 gtttcgtttc caggtcgaca atattacata gaagatctac atcgatatgc ttgacagtta
     6601 ggcctgccct tatgacattg tcggagcaag ccatgatctc aagaatgctg ccttttagat
     6661 aagagtgaat cacaccagcg ggtacgaata gaccttgacc gggctcaagc tgcaacagat
     6721 cgagaaaaag gggagcgaac tgccctggat cacccggata gcgagattgt agctctaata
     6781 cccaatgtgc agcatctcga gctgtctttt gttccgaatg aaggagctgt tcggctcgag
     6841 catgcaattc agtctctaat gctagatatt cctcgcctct aacacaaaag agtcgcttta
     6901 ttaggctacg gagttcactt ttatcctttc ctttaaattc gagagaatcg caaagcgagt
     6961 gacccaaaag agagcatatt tcatatatcg agcgaaatcc tgccatggct tgaaatgatg
     7021 taagtgctac ggccagctcc ggtttatggt gggggtcttt gaatgtccgc tctggagcca
     7081 atcggccaat accagccgat tcttcttttg caaatcctat ttttgcttgt tcaaggtttg
     7141 ggtgaacttg aagggaaaga ggagaagctg cagcgagcac cttgaagaga aaaggcaatt
     7201 cattatattt tttagaaata tgatctccaa gccagtgagc agaatcttgt tttatcagcg
     7261 tatcgagagc aaattccgaa tcatcgagaa taatcttaga tggcgctctt tcgtgacttc
     7321 ccatccagac ctctccaata ggatagcctg gtcgcagctc tgcttccaca aagggtataa
     7381 tccctttagt atcaccccat gcatattgtt gggcagagtt ttttagcaag ctaaggcagg
     7441 gtggagttcg ccgttcggta tagatcgaat tgttcttttc gtccataaga tcaattacct
     7501 ttcattatca aggaattctg ccacatctcg acaaatagag tccagagccc ccatcccgag
     7561 tctacatcta ggaaggcttg agataaaaag acttccataa gcctttttca agatgcgaac
     7621 atcgaggaca gcaacgaccc cgcgatcctg cgagtgacgt ataagtcgtc caaaaccttg
     7681 cttaaagaga attacagcct caggaatgct catctccgca aaggaatttc cgccgcgagc
     7741 atcgatggca gcagccctcg aggattggat agggtcggtt ggcacgcgaa acggaagttt
     7801 agtgataatg accaaggaaa gagtttcgcc cggcgcatcc acaccttccc agaaggattc
     7861 tgtcgcaaaa aggacgctgg aaatgtcatt tacaaaggtt cgaagcaagg aatatctatc
     7921 tttctgcccc tgtctaaggg ctaatatgcc ttctttctcc aatagtggag agacagcatc
     7981 atatgtgtcg cgaagcgcct tatgacttgt aaaaagcacc agtgcgcgtc cacgtgaagc
     8041 cttaagcagt ttcgctaccg ctgtgtttag gtaagtctgg aattcagcac tatctggcga
     8101 cggagcttcg gtgtcgatgg cgagcattgc attcttggaa taggggaaag gcgaaggata
     8161 atttttttgc agaactcgga ggtttttcgt agagtcttct tctaaggtca ggcttggttg
     8221 tcgatgcttc aactttttgt ccggtagttc gagtgccgac gatctatccc ctgatattcc
     8281 agttcccaat aatcccactc ggctccgcca ccattgaaag gagccgttga tcgtgagagt
     8341 tgcgcttgtg caaatgcagc tgcgaatctt gctgaaaagt cgttctgaaa gtaggggagc
     8401 cacttctaga ggagtggcgc tgcatatcac cataggttct ttaggattct tattatcgac
     8461 ttggagccaa aatatagagc ttggctcagc ttcagggttg atgaatcgcg ccaataaagc
     8521 tgccgtctct tctagagacc ggagtgtaat cctgagttct atcacacttt cttcgtcttc
     8581 cagttccaaa gcaattgctt cggagatttc tccaaggcgc gtgactagca aggcgatctc
     8641 tttctcgagg ttctgcagag tcgacaggaa catggtgcga ttaatgccag ataaattttt
     8701 tacaaggatg ctcgattctt tttccgaaaa gcaagtgcaa gcgatagcgt tgaaagaatc
     8761 gaccgatgat tcagctttct cgatttgaag acgagcagtg tcaatcaggc ttgaaggaat
     8821 atcgcccaat tttgagattg aagctaatat cccaacttga agccgcttct tacgtcttga
     8881 aagtcttgag agcagccttt ggatggacct ctttgaaaaa gtctcagaaa atagacttgt
     8941 tgcgcttgct tcgagtgcgt gcgcctcgtc tatcacaagc gcctgatatg aaggcaaaac
     9001 cgtattgatg cttccttctc gagttcgttt tgcctccaag tcagcaagca agacatgatg
     9061 attgactatg atgagctgtg cgctcgccgc ttctagcctt acatgaatta catggcattt
     9121 gtcgtgataa gggcaatgca aggacagaca ataatcgctt tcggagcaca cacggctcca
     9181 tataggatca tcatctggca gagcaaggtc tgacttgtcg ccacttcctc ctccgttgtc
     9241 ccactcgagg atctctctga gttttatcga agaatcggaa aaaagagcat cttcctcgat
     9301 cgcttcatat agtctgcgtt tgcatagata atttcctcgc cctttgacaa tcactgcctt
     9361 tgcttgcttt ttgaacaagg agctcactag aggaaaatcc ttcttatata tttgctgttg
     9421 gagattgata gtagccgttg ataccactac gcgttcgctg tttccaattg cccaggctag
     9481 agccggtata agataggcaa aagatttccc tacacctgta ccagcttcag caacaagaat
     9541 ccctccatcc gagatgatct ccgcgacgtc gtgagccatt tctacctgtg agagccgtgg
     9601 ctcgaattcg ggcatgatat gcgatagctt tccagaggaa tcgagcacag ccgcgatttc
     9661 gtctgcatct aaaggacgaa ttgtcttggg tttgactggt tcagcgacaa cgagtacatg
     9721 ttcgacttca ttgtcgacaa tatagctacc aatgccttca gcccctgctt cggctgctac
     9781 cacaatatct gcatccgaag ggaaaagcat gccggagggg tggttgtgaa taagcacctg
     9841 tgcatgtgaa tgcttttgga agggcgcagg tacagcgctc tccgtccctc tagccaaaat
     9901 atcgatattc tttacaagtc ctttagaatc aagggttcct actgcaaata tttctctgcc
     9961 ttccatggca agaatcgcag acctaagttc attctgtgct tctgtcgaaa accgtgtagc
    10021 cacatctaag attgcggatt gttttttgct cacatggcga gtatagcatg tatattcgat
    10081 tgttacgaat cttcgacaaa cacagatctg ttcttatcga agatctcaat caaggattcg
    10141 atgcccaatt aggtagttgc gaatcttgag gttccaggta tatcctataa agtcacatgg
    10201 attccaaaac cgaacaaggc ataggcttaa gcctgggcaa tacccattat gtgcaacctc
    10261 gctggcgatc tatgcctgca gaggatatat accgccactc aaaaaatgaa gaaaaagata
    10321 gttatgttcg tgccctggct aaacttcaag aagaggatga agagattctc ttgccaccag
    10381 agcgagggcc cattgttttc cgcgacggag tttttcagat cgacatgagc cgcatagaag
    10441 agaaaggcga tatagacctc gagctgaaaa aacttatcga caatattatg aaaccttgag
    10501 gaaggcatct acagatgagc ctacaatatc aatagaaagc gaagtttata taaagcccgc
    10561 tttgcggcca atactgcata caatgtagaa ggcaactgag ggataatttc gatgatcaag
    10621 agctattcgt ggaatgtaaa tgggcttcgc gcctgcgcag aaaaaggctt cctagactgg
    10681 gttgagacga ctgccgctga ttttatctgt atccaggaga tcaaattaca ggagccccaa
    10741 ctgactgatg agcttcgcca tcctatggca tacaacggga agctctacca tagtttttgg
    10801 gctttctccg aaaggaaggg ctattccgga acagggctct atgtgcttcg cgagccggtt
    10861 tcggtgacta agctgggtat ttccgaattc gatatcgagg gcagaactgt catcgccgat
    10921 tatggcggat ttctaataat gagtgcttat ttccccaaca gccaagaagc aggtatgcgg
    10981 ctcgactata aactccgttt ctgtgatgca atcctcgatt tctgcaattt cgaacgttca
    11041 aaaggcaaac atgtgatagt agcgggggat tataacatag cccataagcc tatcgatcta
    11101 gcccgtcctg agcaaaacga aggcaatgcc ggatatttac ccgaagagcg agcttggatg
    11161 gacaaattca cctccatagg ctacattgat acattccgat tgttctgcaa agagccgggt
    11221 agatatacat ggtggagcta tcgctcccca aaagccagag aaaagaatat cgggtggcgc
    11281 ttagactacc actgcgtcga tgaagagttt tcgccggctc tgcttgcagc cgatatacac
    11341 gccgaagtaa tgggatccga tcattgtcct gtgtcgcttt ttctcgatct gtgatagtat
    11401 cgaattgctt tgaaaaaatt gaccggctcg aagtcggctc cttgtttttg gttcatctgg
    11461 aggatttggc atgcgtgttc gctataatgc gccggttact cttacattta cattgtgtgc
    11521 ggctatcgtg ctcatactaa gtcagactat tatgcctgca ataatcccta atctattttc
    11581 aacaccgacg ccctttagaa ccaatgtatt catggattat gtcaaactct tcactcatgt
    11641 gcttgggcat gcaaacattc agcactttat tagcaacttc acgatgatcc tgcttctggg
    11701 acctattctt gagagcacat acggttcggg ttttttgctc ataagcatac tcatcacggc
    11761 gcttgtcaca gggctttcga atgcctttct tttcccgaat gtagtgcttc ttggcgcttc
    11821 tggtgtggtt ttcatgatga tcatgctttc gtctataacg aatttctcaa aaggagaagt
    11881 gccgcttacc tttattctta tcatgattat atatttaggt ggacagatct gggatgctct
    11941 tacaaagcag gacaatatct cccagttttc tcatattata ggaggccttg tcggtagctt
    12001 ccttggtttc tatcataaaa aatgaacgta agaggacaaa atttgaggtg tcattatgta
    12061 tggaagaata agagatgaac tcgagaagga tctcgaaggg cttcgcaccg aaggtaccta
    12121 caaagatgag cgcataatag ttactccgca gggagcaacc attcgcgttt cagatggtgc
    12181 agaagtgctt aatttttgcg cgaataatta tctaggtctt gctaatgatc caagcatccg
    12241 aaaggctgcc gtgagagcta tggaggaatt tggctatggc ctatcttcgg ttcgtttcat
    12301 atgtggaacg caagcgcaac acaaggagct cgagcgacga atagcagatt ttcttggaat
    12361 ggaagatgct attctttttt cgtcttgctt cgatgcgaat ggagccgtat tcgagcccct
    12421 tctcgatgaa gaatcggcga tcataaccga tagcttgaat catgcctcga taatcgatgg
    12481 agtacgtcta tgtaaggcaa agcgttggat atacaagcat gcagatatga gagatatcga
    12541 agaaattgat cctgagactg gcaagccact aaaaggactc gaacgctgtc ttaaggaagc
    12601 tcaaggtagc cacaggataa tgatcgcaac cgatggtgta ttttccatgg atggcgacat
    12661 tgccaatctg aacgcaattt gcgatttagc agaaaaatat ggtgcgcttg tgatggtcga
    12721 cgattcgcat gcaactggtt tcatggggac agctggacga ggcacctggg aacactgtgg
    12781 agtcgggggt agggtagata tcataactac gacctttggg aaagctctcg gaggagcctc
    12841 gggtggtgtc atcgcttcct gtaaagaaat cgtcgagtat atgcgacaga aagggcgacc
    12901 atatcttttt tcgaacaccc ttgcaccagc cattgtaggt gggactctgc aagcactcga
    12961 gcttatcact cgctcttccg agcttagaga caggctcgac tcgaacacta agctctttcg
    13021 cagccttatg acaaaagcgg ggttcgatat aagagagggc gaacatccaa tctgccctgt
    13081 aatgctatac gacgaaaaac ttgctcatag aatggccgat gaacttcttt ccgaaggcat
    13141 ttatgtgata ggcttttcct ttcccgttgt gcctaaaggc aaagcgcgaa tccgcgttca
    13201 gatatcagcg gctcatactc aagagcagat aattgaggca gttgaggcat tcaagaaagt
    13261 ggggaagaag cttagagtga tctaaatcgg atgaatgatt ttctctatct tgaaacttcg
    13321 tttgacaaga ttccttgttg gttagatact ctttaccttt atgcgcatta ttgatatttt
    13381 tattgaaaat taaatcgttt tttctcttag aggtaagaac tatgcctaca tatgaatatg
    13441 agtgcaggaa atgcggttat acattcgaag catttcaatc tatcaatgat gatcctctta
    13501 aaacctgccc tctgtgcgga ggggcagtaa aaaggttaat ctttgggggc agcggcataa
    13561 tattcaaggg tccgggtttt tatattaccg actcgaagaa aagccaatcc gcagctacag
    13621 gctctgagaa atcctcatcg tcttccgtaa attccagtca ttctgaaagc tccaaagaag
    13681 gaaccaaaga gaccccaaag acggatccat ccgagaaagc gagctagaga gaatgcaata
    13741 aatgccttgg ctgagcctaa gctaaggctt tccttagatc ttcaagagat aaatccaaca
    13801 actcaggcct agaaatcaag cgatagaatg tcgatgcagc ttgggaaaaa ataaagccat
    13861 ctgcaatacg ctcatcgagc gttattttta tatctatata gcgctgtctg gttcgcgttc
    13921 ctccatgcca tagctcttta gaaaaagcct tgcctattgc tacaaatatg cttgcggttc
    13981 cccactcata gaggtgatgg aaaggagcat cgagaccaag cgagcccaag tttgcaaaat
    14041 agatgcttgt atagagagga tccatccgca gaagactcca gggtgcaaga ttgatgctat
    14101 caagaagtct gtaaattgtc atcagcactg ctttgccacc tgggatctta tgaaaaatgc
    14161 ctgcgaagcg ttcaccttcg ccttctccat ggatacgagc ttcgagaatt gcatcgctca
    14221 tccttgcatt aatgctcgag aatgaatcct ccggttctag ataaatcttg gcatttactt
    14281 caggggcatc gatagtgaat tcttgcttga caatataaga aaagcatatg tgctttcgct
    14341 gatagagcgc tcgtctatgg ataaagcgat ttagctgtgg gtagagagag attgtcctta
    14401 atgctaccgt cataatgagt ccaaaaagtg taaagcgatc ttggtctttc tttgatgtct
    14461 ggcttgtatt tattgtctta aggtaggcaa gagcattttc gatgtttatt tccttttcat
    14521 aatagacggc agactcatta cggccacgca tcatgtatgg cattaaagca ttgagagcat
    14581 gttggccagt aatacgtatt ccatcacagc ggtctcggaa taacattgtg actcaaatat
    14641 aggagaaaaa aaggcttaag taaactggag taggggtcca gatcgctttc tatcgtactg
    14701 ataaaatgct gtctaatatg attatcgtca aaagagtcct ttacgggcgg tggcaatcag
    14761 tgcacgcgct agatagtaca tgggatctat gaatggtatt tcgatgtctt tttgagaaag
    14821 aacaaggggc acttctgtgc aacccgctac aatggcttcg gctccttggt tctgaagaat
    14881 atgggctgcc tctagtaaaa gatgctttgg ttcattgccg agattacctg cttttatgcc
    14941 tttcgaacca tagatcgcct ccacaaccag ggatcggtgt aaagcttcat caggccatat
    15001 tatgtcctta tcagggaaag ctgtttcgaa aagcattgct tgacgcgtgc ccatcgtggc
    15061 aaggatgcca agattcctaa taggaggaga gaattcctta agggctatgc tggtctcttc
    15121 aaaggcagaa agaatatgca aagtgcaatt atggcggatc cgatctagaa gggcatgggc
    15181 cgtcatacag ggcatgccgg ctatatcagc gccagccgca agcaaccttc tagctgagct
    15241 aagcatagct ggtagaggat ttgtaccacc ttcaagaaga aatttcgtcc tatcggggat
    15301 agaaggatcc gaatccacga tgatatgaag atggccttga tcggaagttg cactatcgag
    15361 cctaaccagc atcgaaaaga aagctgcggt tgcttctggc cccattccac ccaaaatacc
    15421 aatggttcca tttttcatgg cgttataatg gtacccaaga tacccaacta tgtaaataag
    15481 gggagtttcc tctcgtgata ttatttttcc tcttgcggtc ttgcagattc ggattcataa
    15541 tcatagagcc ttatagagat ttgtaaagca aggaggtctg catggctgaa caaaaaaaga
    15601 agggcgcctt ggattggtac tttaagtcca atctactgac gcgaatactg atcggcctag
    15661 tgttgggtgc tattgtcggc atcattctgg ggttctttcc ttcctcagtg aaaccatatg
    15721 tcgacaatac taaattcttt ggagacctgt tcatcaggct actcaagatg attatcatgc
    15781 ctgttattct gttttcgctt attgcaggag cagcgagcat agcaccatct aagctcggaa
    15841 gagtaggcgt gaaaatcatg gtctattatc ttctcacaag cgcttttgcc gtaatagtcg
    15901 ggttgatatc agcttatatt tttaagcctg gaatagggtt taatattgta ggagaagctt
    15961 cggtaaaagg cgtacaagct gctcctccga ctattattca gattttgtta aacattgttc
    16021 ccaccaatcc attcgagtcc cttatgaaag gggatgtgct acctattata ttcttcgcag
    16081 tggtattcgg catcggcatc tcctacatca aggattcaaa agatcaatcg ctggcaaaat
    16141 cgggagaaac gctgctcaat gtcgtcaatg cagcagccga gactatgtac aagatcgtgg
    16201 gtggcatcat gcaatatgcg cctatcggtg tattcttcct aatttcgcat gtatttgctc
    16261 agcagggtgc aaaggcagtt ggacctttgc tcatggttac tctctctgag tatgttggtc
    16321 ttgcgttcca tgttttgctg gtttatggtg gtctcttgac attgtataag ctcagtttta
    16381 agaaattcct caaaggggct aatgaggcga tggtcactgc gttcgtaacg cgttcatcca
    16441 atggcactct accggtaaca atgcgctgtg gtgaagaaaa tctgggcatt ccgaggtcta
    16501 taagcgcttt tactttgcca ctcggtgcga ctatcaacat ggatggcacg gcgatatatt
    16561 taggcgtctg cgccatgttc attggttatg caacgaatca accgctctcc ttcaatcaga
    16621 tgctcacagt agtcattacg ggtacattgg caagtatcgg aaccgctgga gtacctggcg
    16681 ctggagccat catgctgctc atggtgctcg aaagcatagg gctcaaggta gaagctggct
    16741 cagctgtcgc tgctgcctat gcgatgatct tgggcatcga cgcattgctc gacatgggcc
    16801 gcacctgctt gaatgtgacg ggagaccttg ttgggacatc gattgtatgc aagaccgaaa
    16861 aagaactcga tctgtcaaag tggaaataag aggcagagcg aaaagaagtc gagccatatt
    16921 gaagcttggc tagtaatagg tctttctgca tagttcatac aatttagggc gcctaatgta
    16981 ggcgcccttt tggtttagac aggtcattct tgagcatctg cattagacgc ttttttatcg
    17041 agagagctaa tcctctttgg tattcctcct cggtatttca aggatttcaa gagaagagcg
    17101 tgccaggctt gagctgagcg gcgtttaagc ctccgagcct tgtgccttct gccaccgctt
    17161 catggataga gccatggcgt tctagaatgc gcgcgaggcc tgcgccgaat gcgtcgcctg
    17221 agccaatagg attgcggact tttacagatt cgacaggata ctcgcagagt ttttggccat
    17281 cccaataaag cgtggatttt gctccgcgag tcactacgag gtaggtacca tattcgccaa
    17341 agaagcgcct gccttgttct tcgaccaatt ttcgagcaaa ttcctcatcg cgaacattgg
    17401 cgtatgggat tccaagcgtt tgtgcaagtt cttcgagatt tggctttgca cagacaggat
    17461 ggaactcaag acattcaaga agatcctgtt tttttatgtc gagatagaga tgcgcgccga
    17521 catcccaggc tattcgcgca aagcgagcca ttgtgccttt ttcgatacca gccgctactg
    17581 tacctgaaag aagcacggca tcggcttgtt ttgctagatc tacgaagcgc tcgacaagac
    17641 tatcgctcgt ttcgggtgct acctgcaggg attcttccac aagttcggta gtcctgcctt
    17701 ctgtctcttc tatgagagtc gtgcaaaagc gtataggtgc tccagactcg acccattcta
    17761 ccgaaatgcc gtcggctgca cacatggtta ggaaccaagc tctggtaggt ccaccaagct
    17821 gcgtaagatg aatcgccttt tctcctagct gcgtcagaat tcgtgttgga ataacaccct
    17881 tgcctgcagc atcgatgcgc caattcgagg atctatttac ttctcctggc tcgatcttat
    17941 cgaacacaat ggtcttttgg atcgtcggat tgaggcaaac acagagaaaa tgaggaggtt
    18001 tttttgtttt ttgccgtggc tgtgtttttt cgctcgtctg caaagtggga gaggtcattt
    18061 cattttgtag cataaagtca atctatcata caattttagg atgataaagc gttttaatct
    18121 tagcggcata gaaggatatc ttaaaacaag acgccccgcc caatgggcga ggcgaaatga
    18181 gctttgatag cagcttgagc ctcattgctg tactagccca atgcgagtac tggctgttct
    18241 ataagatctc tgaaccgctt taagaatcgg gccccataag cgccatctac gattcgatgg
    18301 tcggctgtaa ggccaatctc gataacggga acaaaagaaa tgccttcatg gccgccctct
    18361 gccggtcgtt ttgtaatggc tcctacagag ataattgcgc actggggtgg attaatgatg
    18421 gctctgaatc tagttatgcc aaacattccc agatttgata gcgttattgt gccatcttgc
    18481 atttcatctg gactaagcga tcttttcctt gctttttcag caagatttac gagttcttgg
    18541 ccaagatctc taatgcctct attgccgcaa tctcttagtg taggcactac aaggccatcg
    18601 ggactatcta ttgcgatcga aatgttgaag tgcttataga ccctaatttc gccatttcca
    18661 agccaggaag cattcaataa tggataatcc gagagagccg aagccataag ttttgctagt
    18721 aaaactgtaa tggagacttt ctcttccttt gatttgaaag cgctttgagc atctatcaga
    18781 ccttgtgcat ttgcctcgat tgacagggtg aactgaggta tctcggtcca gctctgtgta
    18841 agccgctctg ctgctatctg gcgaattctg ctaagtttta catcttcgta cttttgggta
    18901 attggctctg tttcgaaggc cttactagag gcactggcag caatgacatc ttgggcgaca
    18961 atggcacctt gcggaccgct tcctttaagt attgctaggt cgatggcctt ttctttggca
    19021 atggttcttg cgtatggaga agatggtatg aaagtttcag ccataagacc acccaattcc
    19081 ccagcaggct cgattgcaga cgcttgctgg gcaatcgttt tctggatttc cagcttggct
    19141 ttagactcta tctcagcaga agcagaggct gccgaggcat ctggaacgac ctgagtctta
    19201 gaattatctt cattagcaat accttctttt atgctttcct ttataatatg cagcgatgct
    19261 atatcctcca gcgcttcgcc tttttccgca agaattccga ctggcgtacc cacgggcacc
    19321 gtctgtcctg tctgtatcaa gatctttcca agaattcctt ctctttgggc ttcatagggg
    19381 acgactgctt tatcattttc gacatagaag acaatatccc cttttgaaac ggtttctcct
    19441 tcctttttta cccattcggt tatggtgcct tcgttcattg tgaggcccaa ctgtggcatc
    19501 aaaatttcga tagccataga ttagccctcc ttatgtagaa cacggtaaat agcttgaatg
    19561 atatcttctt tctgggctgc catgtatttt tccagcacag ggctaaaagg cactggtata
    19621 ttctttgctc ccacacgctc tattggagca tcgagccagt agaaagcctc attttgaatg
    19681 gttgccgcta tctctgcgcc aagcccgcca atcactaccg cttcatgggc gatcacacat
    19741 cggtgggtct tttttaccga ttccacaata gtcttgcgat cgaatggttt aagcgtgaga
    19801 aggtcgatga tctccacatc gattccctca gcttcgagct gctttgctac ctcttcacat
    19861 ttttgcacca tgatcgagta ggcgacaatc gtgacatcct tacctgttct gcgaatctct
    19921 gcttttccaa gaggtgttag gtattccccg tcggggacag gtcccttttg gggatagagc
    19981 accttatgct cgaagtagat aacaggatca ttctgtcgta tcgctgattt gaggagtcct
    20041 ttagctgtcg cggggctcga aggggtgaca atgataagtc cgggaatatt ctgaatccag
    20101 ccctcgacac tctggctatg ctgagccgcg gcctgattgt ttatgccatc cggagctcta
    20161 atgactaagg gaatattcgc ctggcctccg aacatatagc ggttttttgc aatctgattg
    20221 gcgagctcgt caaaagcaac acctataaaa tccgcaaagt gcatgtctgc aacaggcctt
    20281 gtaccggtca gagctgcacc aagcgctgcg ccaactattg ctgtctcgga tatcggcgta
    20341 tcgcgaatac gatcgccaaa ttcatcgggc aaacccttga attgtccgaa aataccgccc
    20401 tggcggacaa tatcttcacc atatacgaat accgtaggat ctctgcgcat ctcctcgcgc
    20461 attgcctcta gagtcgcttc ggaaaaagaa atggttctca ttgcagtgcc tccgggaatt
    20521 ccttgggcga gaaggagaat aggtcatcga gagcctcttg cggatcaggg aaggggcttg
    20581 cttcggcaaa ttctattgca gcctgtattt cttgttctgc ttgatcgtat agctttgaaa
    20641 taccaacctc atttgctaag ccctcgtcga ggagtctgtt cttatacagt tttatgggat
    20701 ctcgttcgtc gaccattttc ttgacttctt ctcttgttcg atagcgttcg gggtctccca
    20761 cgaagtggcc ttttactcga tatgtaagac attctaggag agcagggcct tttccttctc
    20821 ttgctctccc tacaagataa ttggcagcat cgtaaacttc ggcgacattg ttgccatcaa
    20881 ctgtgtaacc ctctatgcca taaccctttg ctctgtcagc tacgtgctgc gcagaaaccg
    20941 catagctaac tggcgttgtc gatgcatatt gattgttttc gttgacaaag agcactggca
    21001 atttccagat cgatgctaaa ttgagcgctt cgtggaacgt gcctcgattc gatgctccgt
    21061 cgccaaagaa gcacaccgca acttgatcgg tcttttttat ctgcgaagaa agcgcggctc
    21121 ccaccgcgat attgaatcca ccgcctacaa caccatttgc tccaagcatg ccgatagaga
    21181 aatcggcaat atgcattgag ccacccttgc ctttgcagta accggttttt ctaccgaaca
    21241 attcagccat cattctgctg gtattcgcgc ctttcgcgat tgcatggcca tgtccgcgat
    21301 gtgtcgaggt aatgtagtcg tcggtacgca ggttagccat cactcctgtt gcgatggctt
    21361 cttctcctat atagagatgc acaaagccga agattttttt gttcatgaaa agctcttcta
    21421 cgtgagtctc gaatgtcctt atgcgaatca ttgtttcgag catcttcatc ttttcttcga
    21481 ctagcaaaga atcaaagcga ttaggaaagg gatttttcgt ctctgacatc gagcacctcc
    21541 ttatgctcat ggtatgtata attttgaaag aacagggata gcgagagaaa gatcaggtat
    21601 aagcacgatc aagatgagcg caatgaccga tccaattacc ataggaatga caaatttgac
    21661 catttcgttg ttgatgttgc tgtggatgag atttgcggtc acgtacaaat tcaccgcgac
    21721 gggcggcgtg atctggccta tggcgagtga aacagtgagg cagacaccaa aatagagtgg
    21781 atcgatgtgg agcgcaagca atgcgggcat tatcattggc ataaagacat tggttattga
    21841 gattgcatcg aggaacatgc ctgcaatcag catgacaatc atggtgagga acatgaatac
    21901 taactgattt gtggtgaggc tcgaaataag gttcgccgct gcatcgatga cgccgagtgt
    21961 ggcggctgcc cacgagaata cgcctgccat ggctaccacg aacatgacca ccgcagagga
    22021 tacaaccgta tcaacgaggg cttgaaaaat aacatcgatt tttaatgtgt gatagacaaa
    22081 tattcccaca aagagtgcat aaaatacgcc catgatggct gcttccgtag gtgtggcaat
    22141 tcccaaatag atcgacccaa gaatgataac aggcgcgaag agcgcccaga gagaattgac
    22201 aaaagctttt aggatctgct taccgcttcc tgtttcttca gaaccgcggt agttattttt
    22261 tcttgatatg atgtacacga ctcctatgag aaaaattccg accagcacgc ctggaatgat
    22321 gccgccaagg aacaaagcgc cgacagatac gcccgtgagc tgtccataga cgatgaaaga
    22381 aatggaagga ggtatgataa tcgaaagccc cgctcctgca gcaatcgtag ttgctgcgaa
    22441 atatttatta tagccttgct ttatcatggg cgtgatgaag acaagcccnn nnnnnnnnnn
    22501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnct gcgaaatatt tattatagcc ttgctttatc
    22621 atgggcgtga tgaagacaag cccaagcgca gcagcagtcg ccggtccaga gccagaaacc
    22681 gctccccaga agatgcaggc gacgactgcc gcaattgcca atccaccacg tttctttccc
    22741 gtgagcagta tcaagaaatt tgcaatgcca tcgactattt ttgatttatc catgagtacg
    22801 ccggcaagga taaaaaacgg aatggcgaca agagggaatt tcgcgatgtt cgcttcaaaa
    22861 tttctcgata tcatggacat gccaagatcg tagttgagaa ttgcccctat tgcggcggta
    22921 ccaagcgagg taccaatcgg aacacctatc acaataagga ggataaaaat gccaaatacc
    22981 aaaagcgctg ccattagtga gcctcctttt tggaatttac cgaatcagct ggagggggtg
    23041 aggtattaag ggcttttatt tccttaatag tgcttgcgat aatttccttt atcacaaaaa
    23101 gtgaaaatat gggcgtacct aggctatata tccaggtcgg aataccaagc gattctgaga
    23161 atgcatggta gaaagttata ttcttataga tttctcgcgc agaattataa attagaaaac
    23221 agaatatcat gaccccgagg gatgcagata ttatcaatgc cgctcgtttt agattgggcg
    23281 agaacatgtc gaataggttt gtcatcttta gattagcgcc acgtcgaaag gcaattgcga
    23341 ttcctaagag agttatccat acaaatagat tgacagtgat ttcctctgta aacgcaaaac
    23401 ctttgtgaaa gaaatatcgg tttatcacat taacaaaggc aatgatggcc atcaccgcga
    23461 gaagaatggc gctcacgagc tcatcaatgc gtattctatt tttcttcatt gccatggttc
    23521 tccttataaa aaagccggcc ttagaggccg gccgagaata gaaggtactg tacggtacct
    23581 gcatgttgcg gcgtaaggct tgttatttca tgtcttcgag tgcaagttta tatatatctt
    23641 cgccgattgt gggtacccac ttgtcgtata caggttttgt tttttctttg aacgccgcta
    23701 tctcttcagc tgtgagaacg gtaatcttca ttcccttggc aggagctaca acaaatagat
    23761 tctctggatc cctgggatcc ttgaattttt ctttgaaaat cttgagtgac ttatctccat
    23821 cgagatagca tctagcaagc gctttttccc attctccagc ttctagagcc gcttcttgta
    23881 tctgagcctg aatatcgggt gggaaagact tccatacctg ggaatttacg cagaagacca
    23941 gcggatcgat cagataattc caaatggtca tatatttatg gtattcccat atcttattgc
    24001 cgattaagac accttttgga ttctcttgac cgtcgactac accttgctgg aaagctgtta
    24061 ccgcatctcc ccagttcata ttgattggat ctgcgccgag ttgtttgaag atatcgatca
    24121 ttatcggcga tccaacaaca cggattttga gcccctcaac atctttagga gttcttattt
    24181 cgcgtttgga attggtgatc tggcggaaac cgttttctcc ccatgcaagg aacttgactc
    24241 cgcctttttc catttcagct atgatgcgcg caccggattt gccattttct atcttgtcga
    24301 cattatcgta agtattgatg aagaatggca ggctgaagag attgaaagga cgaattacat
    24361 tgcaggcgtt catcgtcgat tctgtagcaa agtctatggc tccatccgct accgcttgat
    24421 accagttggt ctgcttaccc gccaaaagag acgaactcca atatggcttt atattgataa
    24481 ctccatttgt cttctgggca accagatcag caaatttctg tgcaccagca ccccaatcca
    24541 caacttgggt tacatttact tgcatcgtat actctttttt aggagtaaat gccgtaactg
    24601 gtaggattgc aacaagaagt gctacaacag aaagcaataa tttacgatag atcatatgaa
    24661 cctcctgagc gcaattttcg ctcccaatat ttattctgtc aatgtttatt tatttaaaaa
    24721 taaggcatat ttagtatcga tcaaattttg ctacatattc atgcataaca tattgcataa
    24781 aatattatgt ctgagtataa tatgttcagg aagatgaact tattcacgtt gtgctttctt
    24841 gaatttgctc aaagctagcc aatatatgaa taaaagcgcc ctcaggagat aagtcccaag
    24901 gtcttgatac aatagttgta ttttacatat ttattcaagg gagaattaga cttatgaaaa
    24961 agaaaatcgt gcttgcatat tccggcggac tggatactac ggttatagta ccctggctta
    25021 aggagcatta cgactgcgag gtgatcgcag tctgtggcga tgtcgggcaa gaggctgatt
    25081 tcgatgcgat tggcgctcga gcgcttgctt cgggagcatc gaagtttatc aaactcgatc
    25141 agaaagaaga atttgtcaaa gaatacttat ggtctcttgt aaaagcaggt actccatacg
    25201 aaaaaaagta tctcctcggt acgagtgcag cccgcccgct ccttgccaaa ggtctcaccg
    25261 aaactgctct tgcagagggc gccgatgcaa ttgcccatgg ctgcactggg aaaggcaacg
    25321 atcaggttcg cttcgaattg ggagtaaagg catttgctcc ggaaatggaa gtcatcgcac
    25381 catggcgtat atgggatatt cagagtcgag aagaggaaat agcctacctc gaagctcatg
    25441 gaattcccgt gccgatgaag aagtccgatt catatagccg cgatgataac ctctggcata
    25501 tcagccatga aggtctcgat ttggaagatc cttctaacga gcctcatttc gaaggcatgc
    25561 tcaaaatgtg cgtcactccg gaaaaggctc cagacaagcc tgaatatgtg actctcgcat
    25621 tcgagcatgg tgttcctgta tcggtcaatg gcgagaaact cgacccagtt tcgatagtgc
    25681 acaagctaaa caaaatcgga ggatcaaatg gcattggcat tgccgatatg gtagagaacc
    25741 gcgtggtcgg gatgaaatcg cgtggggtct atgaaactcc tggcggcact ataataatgg
    25801 aagcacacga cagattggaa cagctttgcc tcgacaaaaa gacactctca ttcaagatga
    25861 cagtagccca acgctttgca gagatcctct atgaaggaga atggttcagt cctctttgta
    25921 ccgcgctgcg agcttttatc gattcgaccc aggatgtagt aactggccaa gttacgctca
    25981 agctatataa ggggaatatg gtttttgccg gctcgaaatc tccgtattcg ctttatgatg
    26041 caagtctcgc aagcttcacg acagggccac tattctccca caatgattcg acaggcttca
    26101 tcaacctatt tggtttgcca acaaaagtca gggcacgact caatgagcga atcaagaagt
    26161 atggcattgc cgcctccccc gatgtcgtca aaccaggaaa ttcgggctat actgcccatg
    26221 caggagactg atatttcatg gggaaactct ggcaaaccag caaaggcaaa gagctcgatc
    26281 caataatcga acagtttctt tccagcattg agtcggataa gcgtattgta tttgaagata
    26341 tagaatgctc gcaggcacat gctatcatgc ttggcgagca aggaataatt ccattgcagg
    26401 atgcgagctt gctcgtagac gaattggcaa aaattcgcaa agagctcgaa gcaggctctt
    26461 tggccatcga tttatgcgcc gaagatgtac atagctttct cgaagaggaa ctgacgcgca
    26521 ggctcggcga tgtcggcaag gctatccatg cgggacgaag ccgtaacgac caagtggcgg
    26581 ttgcttttaa gctccacatg cgaaatgcgt gcgatatgtg tgaaaagctc gtaaaaaagg
    26641 cgataggcgt ctgcctcgac cttgcagagc agcatatcga tacactgatg ccgggctaca
    26701 ctcacctgca aagagcccag ccagtgagct ttgctcatca tcttctggca tggtgcgctg
    26761 cgttagaacg cgatgcgctc agattccgcg atgcaggcga gcgtgcagat gaaagcccgc
    26821 tcggcgcggc cgctcttgcc ggaagcggat tgcctataga tagggcaaga acggccagcc
    26881 tgattgggtt ttcaaagaca agcgcgaata ccatggatgc tgtcgccgat cgcgatgctg
    26941 cggtagacta tgcggcttgc tcagcgattc taatgatgca cctgtcgcgc ttctgtgaag
    27001 acatgtctct atgggcttct tcggagtatg gttttattgc gatatcgcct cgctccagca
    27061 caggttcgag cataatgccg caaaagcgca atccagatgc tgcagagcta atccgtggaa
    27121 aggcggggag agtattcggc gatttgcagg ccctgcttgt gatggagaaa gggcttcctt
    27181 atgcatacaa ccgcgacttg caggaagata aatcgctctt tttcgatatc gaagacacgg
    27241 tgcagagctc gctcgaggcc tttgctattc tggtccagtg catcgagcca aatatcgagc
    27301 gcatgcgatc ggcgctagag aaagggtata tcgaagcaac cgacgtcgct gaattcttgg
    27361 taataaaagg aattccattc cgcaccgcgt atcaggcggc taagcggctt gttgagcatt
    27421 gcatcgaaga aaaaaagagc ctgcgctcta ttgcacaggc cgatctttcg gtccatgagg
    27481 cctttgacaa ggctgcgatc gatgcaaggg agcttgcgga atatcttgac cccgttgctt
    27541 gtgtttcacg ccgatcccag ataggagggc ctgcgccaga gcgcgtagcc gagcagatcg
    27601 agcgcctgcg aagatttata gcgccttgag gacgcatggt gtattgtatc aataaatcta
    27661 acgagcggag tttctttgac aggattttcg ggataagctt tgacaggatt gtcgggattt
    27721 tacaggatga acaggccaaa actccaaccc aagtatgtgt tttagttatt gattgctttc
    27781 tttggttgcc tcgatcatat tgctatgctc aatgatataa cttaggtaat atgtttcgtt
    27841 tctttcgagc cttttttaaa gaaaaatcga tgaaaagaac tcgctatatt gaatgagatg
    27901 cctgcattgc agcatttaag atgagtcaat tcgcatctag agactgtttt agatgaattt
    27961 aatactcgtc tttcttgcta ttgaggtaca ttgtcgctgc atagattttt gcaatattgt
    28021 cgagccggtc gcattgaata ttaaatcgcg caccgaagtg ttttgtatcg cgataagtac
    28081 caacgatcat tggtatgcca tctcgtcttt caacataaaa accacgctct gcaataccac
    28141 gctgcctatc gtaagggaga cttcttatac tatcaatcca tggctcggag ctccaaatct
    28201 tgtctgaata ttctttgaag cctttaggaa aatgatcatc ggtaaactta ggtggcgtac
    28261 gtttagggga aggttccgga ggaataaaag tagctacaaa ttgcatacct ctttgttgag
    28321 ctgcttctat atagataagt cccgcatgat taatgctaaa aaaatacttt ccattgatct
    28381 gttctgcttc aaagaaaagc ttgatattag aaaaatctgc atttttttga agatcgttgt
    28441 aatcaaggat attatcacca aaatattgcg ccaggtcttc gaaaggaatc gtttcaaaag
    28501 cactgagata acatcgattg aaaaggtcgt tatataattc aaagtaatga aaatcaagac
    28561 tcacgggcat tggtgggaaa tctattatat ttccaaatga ttcatgtttg tagatcacat
    28621 ctacgccaag cgcttggccg aacataaccg caatcgcaat ttgcgcctta tacccccctg
    28681 tcgcatcgat aaaaaccttg gttgctcctc ctgcctcttt aattgccttt gcaatacagc
    28741 gcacgaggtt gattagtcca gaccttttga agagttgagg gtcttgtggt tggagatctt
    28801 cgacagtcca atgttttact tttagaatag caagccgctc atccctcatt gcgcgttgat
    28861 caaaatattc ctgaagtaca aggccagtat ttgcgccatc ttgcgtatcc gagacaagaa
    28921 aaattatgct ctcaagtcta tcgccttttt tctcaatgct ttcgagaata agctcgattg
    28981 tgttgatctc agcaccgagc attcgttcgt gtggatcggt tttgagcatt tcttgagcaa
    29041 ggcctttcca atctttttta gaatatgcaa catataaagg atcgaattga tccgtgattt
    29101 tctccaaatt gacattaaaa aagcttgtgc caactgtgca gataagagtt ttatccatat
    29161 ctatttatcc cctttatagt tatccaataa tcaatcaagt aaatatcaaa tttctgagct
    29221 ctttctgtaa ttttctacta ttgctgagaa ttggcagatg gggtgttttt aattccgaaa
    29281 tcctctgctt attcgtttgc tttctcttta ttgattatat caaacatttc aagcattgat
    29341 agtgctttga gcctgaactt tatccgcagg tcctctttct tatagatggt gtcgacgaga
    29401 atataatcat tttctagata agcatcgatt tatgccttgt attatataca aaaaaataaa
    29461 ttataattcc aaattattgc aacatcctat tcattggaca ctcttgcttt tgccacaata
    29521 gcaaaactca gcgccatatg atcatcttgg agattgggta tagtttctct gattatgttg
    29581 ttgggccatt ctttaaacca gcagattaac agacacatta taagcttttc cttcaatgcg
    29641 ctgctatcaa agatttctgc gaagaacatc aactgcatcg ccttaatatc atcatcatta
    29701 ggctgtcatc gagtcttgtt tttaagcatg atgcgattga gaatttggca tcaatgagga
    29761 tatttcttta ttcaattaac ttaggccttc tttcattgca cagctcttga aagagacgct
    29821 attcctttgt ctacttatga tactattaag aaaaccccgc aaataaaaaa tttgttttta
    29881 agtatcattg actgaagaat cctgtcgcct ttgtgaacga atgctgtacg caagaaaata
    29941 gttatctaat aaaatgttgc cctaaaccat ctgattaata ggtacaaata tcgacaaact
    30001 aatttcttgc tctggattag taatagaagt taagcgtcga aattgtatgc tcttgactat
    30061 aggcttcgaa gaatgaatag ggaccaatgg aaatgatttt tgttcacagg gtgatatcgg
    30121 caagaatctc ctagaggctg atattattcg cttttcaatg tctagtctta gtcccttcta
    30181 tcaggtctaa atctcaaaac gagaatcagc ttaggcaaat atgcctacaa tagtaatcat
    30241 cttaatccct tctatcaggt ctaaatctca aaacgtgtgg gaaaattacc gtatgaatga
    30301 tttgcaccct atcttaatcc cttctatcag gtctaaatct caaaacccaa gcgtctcgga
    30361 gatgatgaga tgcgtggtca atatcttaat cccttctatc aggtctaaat ctcaaaacac
    30421 tcttcctaat tcgacagaaa ggaaagtatc tagtagtgga ggtcttaatc ccttctatca
    30481 ggtctaaatc tcaaaacacg caattgacct gtcgttgttt cctagacctg ctgctgtgtc
    30541 ttaatccctt ctatcaggtc taaatctcaa aacggggcca agtatggcta caattacaaa
    30601 gcgagatttt tgtcttaatc ccttctatca ggtctaaatc tcaaaacggt taggtatgcg
    30661 gtcgagaacg gagtaccatt cggcgtctta atcccttcta tcaggtctaa atctcaaaac
    30721 cgctaattgt gctgacgtta ttcgtcgaga tagtgattgg gtcttaatcc cttctatcag
    30781 gtctaaatct caaaactgga acttgggctc aagccgaaga tggtggactt cagcggaagt
    30841 cttaatccct tctatcaggt ctaaatctca aaacctatga tgtgagatat gctcaaaagt
    30901 tgtggatggg aaaagacacg gacgttcatt tttgataggt tattgtgatg aagaagacca
    30961 atcaaaagga gaacgtccgt ggagaaaagt atagcagaag gtttgacgca aacaaagcca
    31021 gagaccgccc aggtgctgga agagcttgta cgggaaggag cgcggaagat gctacaggca
    31081 gcgcttgaag cagaggtagc ggagcattgt gcaaagtatc agcatctaag ggatgcgcat
    31141 gaccatcaag aggtggtaag aaatggctat cagccagaac gaacaattct cacaggagca
    31201 ggagctattt cgattcgtcg gccacgggtg gacgatagaa tactgagtac acgaggagag
    31261 gaacgtttta gtagtcggat actaccgaag tttatgaggc gggcagcgag tctcgattca
    31321 ctcataccgg tactgtatct caaaggtatc tccagtgatg attttcccac tgcgctcgaa
    31381 gccatacttg gtcctgatgc aaagggatta tcagcgacga cggtcgtgcg gttaaaagag
    31441 gtatgggaaa aagaatatga gcaatggaca aagcgagact tgagcgccaa gcactatgtg
    31501 tatatgtggg cagatggagt ctattgtaat gcacggcttg aagatgagcg ctcgtgtctg
    31561 cttgttatta taggagctga ttcattgggc aataaggagc ttgttgcggt gtccgatggg
    31621 gtgagagaga gcacgcaagt ctttgagaga gcttctgctt gacttacgag caagggcatt
    31681 acggtatgca ccttccttgg tagtgtgtga tggagggatg gggtttcagg cagcagtggc
    31741 agaagtatgg ccgagtacgc acattcagcg gtgttggttt cacaagatgg gcaatgtgct
    31801 cgataaaatg cccgagagtc ttcaagggaa ggcaaggaag atgctccttt ctcagtatat
    31861 ggcaccaacg aaggaagagg cactcaaggc atatggtctt tttatagaaa cttttaggga
    31921 taaatatccg aaggcagtgg aatgtctcgt aaaggacaaa gaagatctct ttgcatttta
    31981 cgattttcct tctgcgcatt ggattcatct taggactacc aatcctattg aatcaacgtt
    32041 tgccacagta cgattgcgtc agcgcaggac aaagggcaat gggacaaggc aggcaacgct
    32101 cacaatggtg tataaacttt gtaaggaagc tgagaagaat tggagaaggc ttagaggatt
    32161 caatttgatc ccgctcgtcg agtcgggagt acgatttgtg aatggagaat tggtcgatga
    32221 agcaattgcc taattcaaga ttcgtccaaa tttcctatcc acaactattg acaatatctc
    32281 ctatgatgtt ctgctagtct attgagtcta ttggctatta ggtcttaatc ccttctatca
    32341 ggtctaaatc tcaaaactgg gaaaagatac gactatctca cccaggaagg atggtcttaa
    32401 tcccttctat caggtctaaa tctcaaaaca cggcagccaa gaaagctgag aaagaaacac
    32461 ttaaaaagcg tcttaatccc ttctatcagg tctaaatctc aaaacgtaat tattcgagta
    32521 gggaaaagtg atcagcctga tcttgtctta atcccttcta tcaggtctaa atctcaaaac
    32581 cagacaaata gagaagcgcc aaaaagtgca tggagtgacg ggtcttaatc ccttctatca
    32641 ggtctaaatc tcaaaactat gaagatgatg gctacaggtc tgattttgcc gatcccgtct
    32701 taatcccttc tatcaggtct aaatctcaaa acggtgatgc gcttgactat agcagcgcgg
    32761 ccttgctttg tgtcttaatc ccttctatca ggtctaaatc tcaaaacttc gcaatttatt
    32821 aaagaagcaa cgacagcttg gatgtcttaa tcccttctat caggtctaaa tctcaaaact
    32881 cggctggcgg cctgggagca gactcagcca agggtatcgg tggtcttaat cccttctatc
    32941 aggtctaaat ctcaaaacag aagttattgg cttataaaga ccaatggaac gataggtctt
    33001 aatcccttct atcaggtcta aatctcaaaa ctttattttt gggcaagtaa tggacgggat
    33061 tttcttcgtc ttaatccctt ctatcaggtc taaatctcaa aactaaaagg caagtttagc
    33121 ct
//
LOCUS       DAOSOB010000013        32947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1378_length_32947_cov_68.586678, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000013 DAOSOB010000000
VERSION     DAOSOB010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 32947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32947
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1378_length_32947_cov_68.586678"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..758
                     /locus_tag="PLS88_02555"
     CDS             54..758
                     /locus_tag="PLS88_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOU06264.1"
                     /translation="MESGCKSNNITKIRAIKLVFVASFCVALFFVISKTARLYETKLI
                     YKSDNKSSSILFSINLGNIYDYDDPISKLYSNPKTKDEVLKFFSTMTNSERVAKAILD
                     NAVAHNMRPSLAFAVAYVESRYNPRARSTNRTGTLNSGLFQLNSSVYGKVNEKLIYDP
                     YQNAKLGIGHLQEYINLNGDEFSALAAYNAGITRISEKGIPDVTFKYISEVSIMEHKI
                     MNFFIASIALSESMKL"
     gene            complement(744..1385)
                     /locus_tag="PLS88_02560"
     CDS             complement(744..1385)
                     /locus_tag="PLS88_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU06265.1"
                     /translation="MSAQVAHEKRKQEILEKALDVFIEEGYADTTFQKIAERCGITRT
                     ILYLYFDNKKQIFGESIKRFLSSLETQIALIAQDQRLTSGEKILKISEILVEACERES
                     KMLSVVMDYLLRLKASGGNPDEKVRRRTVRMRHILADIVIEGIKSGEFKESSSVKGTV
                     EMFFALVEAAVFRLVVLGRKEAAGLQEALSPFVDNMISPTFERNREQNESKLH"
     gene            1515..>4466
                     /locus_tag="PLS88_02565"
     CDS             1515..>4466
                     /locus_tag="PLS88_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU06266.1"
                     /translation="MALQILRTLRSQNIPPKYLWTPSGAIALPDAVSARFVAQKYKLS
                     AGELRALSLIDEAFRIIIDLYRKQYSKLLEEIALKAFASTEESKALWEVLQELIIEFP
                     PAPIYDGLAEPKDWLESLSPVGDGSNRPNRELIIEQLILIRLFNENPACSPYRSLFDD
                     GVSPTGITLPGSISAKTPYLQVFARLEDALKTLPGLSYGDGKTLDLINFLREPARNAP
                     TSLKAQLEWIIKNWGTLLGDFRLNLLAGIDMINEETRSRFPPGPGPAVPYQYTSNLHE
                     YEKFSPDKNWMPSLVLLAKNVLVWLHQLSKIYSREISRLDQIPEEELIIMAERGINGL
                     WLIGIWQRSPASEKIKKLCGNSEAAASAYSLFDYEISPELGGWEALDIFRERCGQYGI
                     RLAADMVPNHTGIDSLWMRTRPELFMSLSYCPFPSYSFNGPDLSGDPSIGIWLEDHYY
                     NRTDAAVVFKRLDRHTGEVRYIYHGNDGTGMPWNDTAQIDFLNHASREAVKERILYVA
                     AHFNIIRFDAAMVLAKQHIRRLWYPAPGSGGAIPSRSEHAISDEAFDKAMPNEFWREV
                     VDLCAEKAPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNMLKDEKNSLYRLTIKNT
                     QEFDRDILKRFVNFMSNPDEETAVAQFGKGDKYFGVATMLATMPGLPMIAHGQIEGFT
                     EKYGMEFKRSYWDETPDRDLIARHEREIFPLLRMRPLFSEVENFYLFDYMRDDGTIDE
                     NVFAYSNGQGEQRVLVFYNNHWERTSGRIHSSSAFAQKTTDGKKHLKTVSLASALKID
                     SSPGNYVIMHEIRSGLWYIFRSEDIASHGFKVALEGYQNKVFLDITNVHDTEGRYTRL
                     FEIIDSRGIADLDDALLEADQPELYRSLHNAISSLSSIETIQNLSKEEAIQRATIFSE
                     IFFSRLCEIAGSDDTLAASRSDSVRAASSWLKIVLKLLYGMSESDSGTSTAATVLGHG
                     GSASDSEYQQYR"
     assembly_gap    4467..4566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4567..5188
                     /locus_tag="PLS88_02570"
     CDS             <4567..5188
                     /locus_tag="PLS88_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06267.1"
                     /translation="TVLGHGGSASDSEYQQYRLILLIYSFMRALAWAFAADGLNEEAA
                     RIVKEYRISKKLTELAVGLSRRSDNRDAEYHASICAEIAVAWALRQNSLLFDTKGTIN
                     SIQAPDKRANEICVWAFSNPLMREALNINQYRGIEYFNKERFEAFISLLPAFAWIDSI
                     QEETKEKEWKEYLSWKEVAEILRENAIATGYRTATMLEYMASIAQA"
     gene            5235..8120
                     /locus_tag="PLS88_02575"
     CDS             5235..8120
                     /locus_tag="PLS88_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOU06268.1"
                     /translation="MPKSVDSTIIAEQYRSLFETPLGRDIVQNLQDENVVFVFPSQDT
                     ADSWAQAIVKSEITRAVALDRFLGFENFLLYVAKANEHDDLCALKPIDRWIWALQMLS
                     KEHNIGDKRPNKGLSALLPSLPTMEISTKNLARLVRLVPSLFEIESLTKGSTALDISS
                     YRFIEEELEAIDLLAEDYFDFLDRSKLIDAHSCPLDLPKSMKVRAYGLKDDFIRFGLI
                     DTKEPSDRNIGCNGIFSEKASTKESLKANSQIVFPDIITQERAIKTSILYREFSSSLD
                     EFEWILEEISNEIEQGIEPEDIAISVCGLNAKKAAWIRQIATEYGVPISIRRGKPLSI
                     SPFGRLLVAIQNAAREGLTLESLDAFAELANITKRDPEGWKALRDTALHLHLPSPSPN
                     AEYIHSLWKEAVKTGQCPESTAQMYQRLWESILFISKASSFSKLYDYILGFLERWVDT
                     SGFGANIQTDRSMRLALDELQGWIDHEYISPNLSIPPFELFIAVLESKSYIPLVEENA
                     VHVYDFDTTAGIAALSHYIAGASQEGLAPSLKLRSALPPELAGLIHQEKTEKVYEAIN
                     LHQVSPTQFSFATESFEDFEAAYPLFPPPVKTASHTKHRLRPILVFEEQKTATKNYSH
                     PKPIETAIARRFLDETKSIDLEKNYSIFSPSSLEDRSRCGFRWFASRMRLSDVYARDD
                     SAIAIGNFYHRAYRLAIQRLLRNHKDGQAEDEFQRALNSALRIEAERILLEYGNGLRP
                     ILSALVPRASHRLHELWNFEQKVFSAYEREGFEVELRNEFTDEEAILGGRIDCLFSRQ
                     DESLGNIKCYVIVDYKKNTIPSISKMKLSAMPSALDELVSSDETQEEEVNGYNDSALL
                     LQEIQIPSYALLLETSGGRIDGAFYWSIEKAEALGYIKPPTVPKMKSAYTYSNETGDV
                     RSTLRDMLRTASSAVKAGNFLDPALDRAVCEDCQFKPLCRYWYFLEL"
     gene            8120..11824
                     /locus_tag="PLS88_02580"
     CDS             8120..11824
                     /locus_tag="PLS88_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU06269.1"
                     /translation="MKPQQDSFDLEALKLDDFQKRAAYTEDTAIVTAGAGAGKTRALV
                     GRFLYLVLQKNVDPERILALTFTRKAAAEMFQRVQLTLSPFANDSPALAKSLVQAHIQ
                     TLDSFCREIVAQAATLYGYTPDFKIDESKCARIAEKTAYRYVLDNQKENGLESLVATF
                     GFEAVVQDLFASFGKANVEPNWQGKQICRSSAQIGEQEFRKIAAEISKKIHDIALNIL
                     EIAHREPNHDFKEGTNQAIRMAKAVSSFSCDLTGLRDFLDYMEKPENALNMRSIGRNE
                     TESAIKELAKELKDKSFQDKISQVLDFEEFLPTYYAIMNRLDEYADMLCEEKCIANIM
                     NYKDLGALAVDILSKHEDRRIFWASRFQYILIDEFQDNNELQKQLLFLLSKEGDEIRR
                     GKLFFVGDEKQSIYLFRGANVSVFKGLADELMAVPLSLTRNYRSTSQLIEFFNELFRI
                     VMQPANPENPQPFEARYEDMETRSIADSNKEEPQGFSSRIEYHCITSKTPLEEAGSLY
                     SVRESMAFRLALWIRNAVESSNPLFIRNEKQTQDPAAREANHSETSLRKAEYSDIAVL
                     MRTTSRQYEIEKYLRMLAIPFVSDTAASLFSEEPINDLYYLLRLLVDADDLFATAAVL
                     RSPLCRISNDGFVSILTEKKSLADLEAGIPKELNPNDKNSVERMISFYRELRPMSDHM
                     PLMELIAYIWKNAQLEASILCDEQRLPYLEHWNAIRTIAASIEAEGGHIAAFLISIRD
                     YMQNMHMFDTSSVPKAEAHGVHLLTIHKSKGLEFPIVIIPWTEASTNRNSGKELWGIL
                     ESDQEQGKTQFYTVDIGFHDRIKSGSNILQTQAKALRTEKEQAETKRLLYVACTRAID
                     HLAFFCAAPEKRSYDKDSFHALLFKDKSHLLQQDSDDKTQLPSHLSDLRLFFEPLVSD
                     KEIQRVRSHGLIERKLSQKELLSQEEAQKLAPTSKETSCKIASKKEEVLAEHAIMTNL
                     TSNSVANHHSRHITISSINAIISKETLAADYARELSKHFVREHIFIPLEHQALSETKA
                     PDKVLLEVKNSWESLNSSAYGSLVHELFAHLLAGKSIESYRPSVSISRQLLGNTSATD
                     IDVSNTNKATHRHMRFLIENAARELAPFFEAPRFSTLILEKKYSCEFPFLLGLGRCII
                     EGRMDFIAQRDEEVIILDLKSDQRFSPYEYSLQLALYIMAARQLFQKKKVRAGLMYLH
                     FGEIAWLETEPEEKTILRICDSISFDKHNN"
     gene            11841..12425
                     /locus_tag="PLS88_02585"
     CDS             11841..12425
                     /locus_tag="PLS88_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOU06270.1"
                     /translation="MKIPSKGKGPNKTIKKTEPTPLRIFLTLAQINTLTLGGGYVIVP
                     VIGNSFQKQGWIDEEEFYRIFSRAQVFPGPVALSTSFLCSYRIAGVKGAIAAVLGVVL
                     PPFIALILVGGFISLYGDSELFKRFLNGAGAVVPGLVGSMLWKTAKNRKWTARVFIEV
                     GILAILLILFPSAAFFILIGGIVALYLGRLLWKS"
     gene            12413..12925
                     /locus_tag="PLS88_02590"
     CDS             12413..12925
                     /locus_tag="PLS88_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOU06271.1"
                     /translation="MEILKISLAFFKIGIVAFGGGWSVVGLIKQEIVPNWMSADAFSS
                     LIAIAQSTPGPIALNAATLVGWQKAGFLGALAATFSVIAFPLLAMSITMAFSKYIPLK
                     KDLADESLRSGSMAMIIMTLWTLLPQNTLPGPWIFALASFGVTAFTKVNGVWVILGAG
                     AINMLLGVLP"
     gene            complement(12972..13925)
                     /locus_tag="PLS88_02595"
     CDS             complement(12972..13925)
                     /locus_tag="PLS88_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOU06272.1"
                     /translation="MVIPTYPEFAPLTLDARDILYPALNLLPDGISEFTFSGLYLFRN
                     TYGYEISQLTDEKFIISGRYKGRTFFYIPCCFPSMEQFDELMLHFDYMRHLSECQASK
                     KRIELERRGYLVREDRDNFDYLYNREDLATLNGRIYHKKRNLVNGFVSNYACEQRPLT
                     KDRTTDALLVLEEWRAAKGAEGDYAAAREALECFDILGLRGAVYYIDNRPVGWCLGEP
                     LAKGKMFAIHFEKAIDSYKGIYQFINQAFAQSLPKHYLQINREQDLGDLGLRQAKMTY
                     RPVGFVKKYTVERPELANVVPAPVEPPAECGPGTVHACDED"
     gene            14084..15340
                     /gene="pepT"
                     /locus_tag="PLS88_02600"
     CDS             14084..15340
                     /gene="pepT"
                     /locus_tag="PLS88_02600"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOU06273.1"
                     /translation="MIAILDYLNRSYTEEMLNRFLSYVKIDTQSNRHADKTPTTEGQW
                     DLAHKLEQELLELGISELELDSNCYLIAHLPASPGKEHVPCIGLMAHMDTASDVSGSG
                     VQPRVIKNYDGKAISLSKQYMLDPNEFPDLCEHVGDTIVVTDGSTLLGADDKAGVAEI
                     MTAIAYLLQHQELKHGPIDIYFTPDEETGKGMDLFPLEKARAKACYTLDGGAAPEVEA
                     ECFNAYVVKADFFGKVIHIGAARGKLANAVAMAGSFIDMLPRSESPEATDGWYGYYCP
                     VEVSGGLEHAWAEVYLRDFSASRMQERIAALKAFAKAVEAQFPQGHIELSVTQQYLNM
                     KEKLVNHPEVLEKLKKAIKDQNIEPIMKPIRGGTDGSRLTEMGIPTPNLFTGGYNYHS
                     RFEWASLSDMALSVETLLNLVQVWAD"
     gene            15610..16161
                     /locus_tag="PLS88_02605"
     CDS             15610..16161
                     /locus_tag="PLS88_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06274.1"
                     /translation="MFSPLDFKEYEQVASNSGAFKGVEIDLMLEILKDWQQAPGKSYT
                     LLELRDGKTLVAFAIIHKVAGRNFTFDIRFVVLDSDYRSTNAIQHLFEMIDQELLKKM
                     PFAVIRFELSSDKRDSLGENTLENSGYCQIGHITEFYGANDDFFFYIKAIFRNPPNFI
                     KISKPFEDELSPESLPEETNNQQ"
     gene            16171..17097
                     /locus_tag="PLS88_02610"
     CDS             16171..17097
                     /locus_tag="PLS88_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU06275.1"
                     /translation="MIVGAIEGGGTKFVCAVLKFDDSNIQEKPPRILKIERILTTDPE
                     STLDAVVRFFKNSGIECAGLGMFGPIECRIESPRWGYLLNTPKPGWSDIDIAQRLQKQ
                     LQVPLKLETDVAASGIGEWKWGAGRGTHCCVYITIGTGIGAGIIIDGIPVRASFHPEM
                     GHMRVPRARTIESRLDEFEGICIYHSDCLEGLASGPAIAARWREAPENLPTEHPAWRL
                     EAEYLAFALTNIALTIAPDRIIVGGGIGLREGLLDMVGKRMRELLGGYIQALSSAEAI
                     RLFLARAKLGNEAGVLGAAELARQLFNSVSKR"
     gene            17231..17303
                     /locus_tag="PLS88_02615"
     tRNA            17231..17303
                     /locus_tag="PLS88_02615"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17264..17266,aa:Thr,seq:cgt)
     gene            17317..18588
                     /locus_tag="PLS88_02620"
     CDS             17317..18588
                     /locus_tag="PLS88_02620"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOU06276.1"
                     /translation="MNSIVHRFGRAVSSQVPQNPQLSRNILAAGYTLSGWKDAYFGGK
                     GLKGVYAHMNAAISRNMSHSFRDSSRSAMVNIFFPCEILYAMDIAPVFPEALSVYIVN
                     SDCGEVFAESAEAIGISESLCSYHKIMIGTARTGVLPKPLLIANTTLACDANQLSFRY
                     LAEEYGSPHFIVDVPHTVNKESVHYVASQLKNFTVLLEDLLHSSMSESKLRYTVDRAN
                     RTIKAYREYLNIRARTSLPISMTGELFSLVATHVFLGTPESEQYAIAIKELAQKASPD
                     RRKKRILWLHTLPNWQDSIRVLLENNERTEILASDMTYDTLIDMDPEKPYESMAERLV
                     YNINNGGAQRRIDAAIAYAKKLHADGVIVFGQWGCKQTMGMSQLAKMALEEEGFPTLV
                     LDGDGCDPRNVADGQMATRVNAFLEQLEASR"
     gene            18585..20321
                     /locus_tag="PLS88_02625"
     CDS             18585..20321
                     /locus_tag="PLS88_02625"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOU06277.1"
                     /translation="MIGYTCKYTPIELLYALGETTELLNHEVTDFEYAEKWTHPNLCC
                     HAKAFLLHSSELNELVLINCCDSIRRAYDVLLAQKSHRFLFLFDLPHIDDGCGQENVK
                     KELVRLTQRYIEYHPVSFDRQRFLKACRASSVSSFDYASDAFFEAPSEATSKLKELDS
                     AATFIAIVGARVSQELLSTIKEKVPYPVIDATCGGNRHLGLLPPNAENISFDELMDWY
                     AKELLHFIPCMRMANIEPRRALWENENLCGIIYNTVKFCDYYSFDYSRVKKEASLPIL
                     KIESDFTPQTEGQLSTRLEAFLENIQARQKKPQKPKKQQLRHSDLFGGIDSGSTTTKM
                     VVIDRNRKILASSLVSTGAQTQKSAQKALQETCRQLDIEPVDLASIVSTGYGRNNIAF
                     ATDSKTEITCHAKGAFFLDPTIRTIVDIGGQDSKIIRIDNQGSVVNFVMNDKCAAGTG
                     RFLEMMARALELDIDNMVRTGLAWKSDLTISSMCTVFAESEVVSLIADNRSIADIVHG
                     LNKSIASRITSMISRAGGQAPYMMTGGVARNTGVVRELEIKLGERLIISEIPELCGAL
                     GAALSALELMEG"
     gene            20370..21236
                     /gene="mtnP"
                     /locus_tag="PLS88_02630"
     CDS             20370..21236
                     /gene="mtnP"
                     /locus_tag="PLS88_02630"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOU06278.1"
                     /translation="MEKAKVGIIGGSGLYEIQGAKVVEEIKMDTPWGSPSDDITIAEI
                     EGVKTAFLPRHGKGHFLLPTEVNSRANIAALKMLGVREIIAFSAVGSLKEDIHPRDFV
                     LPSQIIDRTKGRSGSSFFGEGIVAHLSFADPFCMRLCSIIEPFILARGLRLHTNETLI
                     CMEGPAFSTRAESQLYRSWGAGIINMSVLPEAKLAREAEICYAMVCMATDYDCWKTDH
                     ADVTVDMVVQNLNANSAHAKALILEVVPALSKDAECSCHTAMRGAIMTAPSKRNPETM
                     AKLRTILPELEL"
     gene            21305..21736
                     /gene="rpiB"
                     /locus_tag="PLS88_02635"
     CDS             21305..21736
                     /gene="rpiB"
                     /locus_tag="PLS88_02635"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOU06279.1"
                     /translation="MKILIANDHGAVALKFHLLRWLESQGHQVVNLGVDTEDRVDYPD
                     QAALAVKEYFGGSYDLGIVCCGTGIGISIAANRFKGIRCALIHDSYTARMAKEHNNAN
                     FLAFGGRVDYKEPIEELLSVFLHAEFQGGRHAERVSKLDKI"
     gene            complement(21733..23154)
                     /locus_tag="PLS88_02640"
     CDS             complement(21733..23154)
                     /locus_tag="PLS88_02640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU06280.1"
                     /translation="MSISICSLGAAEEVTGSKHLLEVDSTRILIDCGAFQGKRAKADN
                     KNRALLGDDIEASSIDALVLTHAHYDHCGLVPYLAKKGFTGNIYATSATRDLANLIMS
                     DSAHIQARDADYLSRQAEKRHEKFDWKPLYDDFDVIRAMDQFVTVSYHRPISIADGIQ
                     VEFLDAGHILGSTFARITAKDKAGNVAILGFSGDLGRKGKPIIKDPECLDKIDFLILD
                     STYGNRLHESTMDALRHLEDVVNRTAERRGKLIIPAFAVERTQELIFCLHLLHDQGRI
                     PDIPIWVDSPMALNATSIFAIHPECYDKETYDLFTTHAKNPFGFSALNFSRSVEDSKA
                     LNQAKGPLIIISADGMCEFGRIQHHLMHGLGDSANTVLIVGYMAEGTLGRRLRDGAKE
                     VRIQSDWFQVRANIEEIDAFSAHADWKETVEWLDCIEKERLKGTFLVHGEPEALMAMQ
                     GHLQDAGLKDIEIAKMGVIYKIS"
     gene            23212..24384
                     /locus_tag="PLS88_02645"
     CDS             23212..24384
                     /locus_tag="PLS88_02645"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOU06281.1"
                     /translation="MCADTLTLRVEKLSSNGEGIAFSEGKAVFLPFAIPGETALAEII
                     EEHSSFSRARLIDIEESSPYRVEPFCPHFGDCGGCTLQHIGYEYQALLKKQAAQGTFI
                     RIGGFDPGELQIITGESYHYRNRAQIHACGDGGLGFAKTGSHESVRASHCPALVPIIE
                     RWLISENRKAHPYRALSALIGDRPRFVVFGQEDRLYIEGRDGFGKAKVAGKELKFPLA
                     SFFQSNISMLEKLIEREVSPLKGKRALDLYCGAGLFSLFLAERFEAIDCVESEAASLE
                     AARMNLESTKAKIEFSCTTVEHWTEKTPTDLSFDYIVADPPRTGMAPQVRTWLSFVDA
                     PALLYISCDLASLARDLRDLFQKGWQLENMTLYDFYPQTGRLEAAVHLKKEAKHDL"
     gene            24374..25951
                     /gene="pyrB"
                     /locus_tag="PLS88_02650"
     CDS             24374..25951
                     /gene="pyrB"
                     /locus_tag="PLS88_02650"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOU06282.1"
                     /translation="MTCNGPFKGRSISVVNDLSLDEQLYLYRKARELKETARAGDDVE
                     KFRINDKDYQVYLIFMENSTRTRESFRNAGKFLGARVNVFDAATSSFNKNESITDAIK
                     MLFGYSDESCFILRTTLEGVCTWLDQAFTEYAHITEKPKPSFINAGDGKHEHPTQEFL
                     DEFSFLEQLNWKEEHIHVAMVGDLYHGRTVHSKADGLRIFKEVEVDLIAPPLLSMPSH
                     YVEKMRSNGFQVRTFESIDEYLAQDKVAPIWYFTRLQLERMGEAVLERAPYLRQSVTF
                     KKDYLGRLPEGCRFYHPLPRDRNFPTIPFFLDDLPLNGWDSQSINGYWTRIIEIAMLS
                     GRIGEDFEGEHAKPREFIDDFVHEAPIQETAKPEYKVGIKPVEEGIVIDHIARGAPIG
                     EIWDTIDAVRKILKLDVRSSHGVYHSNREGIYKGIISLPDIISFGEKDLKKLAAIAPG
                     CTLNLIRHAHVAKKYRLSMPPRIYNFDEISCKNENCISYPAHHEGVPTEFIRKGETTF
                     VCKYCEREHKFRDIWDI"
     gene            complement(26080..26823)
                     /gene="rlmB"
                     /locus_tag="PLS88_02655"
     CDS             complement(26080..26823)
                     /gene="rlmB"
                     /locus_tag="PLS88_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOU06283.1"
                     /translation="MKYLTSFHAILETLKAENSRCSLYITENAQRRGPRIREILDEAL
                     SRNISIQYVSESRLEEMSKDQRGAILALDEVLKGKPSLKALCDRVSEPEPSLVLVLDH
                     IEDPQNLGAILRSADAFNVDAVIMPERRTSPISNAVARASAGAIAWVPILRVSNLRSA
                     IDTLKKAGYWVYAADMGGKPLVSAGMAQKAVIILGNEGRGVSEILRKVADDIISIPMQ
                     GHVESLNVSVSAAILMYEFWRSNSGSSNI"
     gene            complement(26875..27696)
                     /locus_tag="PLS88_02660"
     CDS             complement(26875..27696)
                     /locus_tag="PLS88_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOU06284.1"
                     /translation="MAVIAISRELASYGEETAQELSKLNGYKIVDREHIEAALSAMGI
                     DVEKQARYDEKNPGFWASLSQQRDEYLHFLTQTVYETALENNCIFIGRGAHVILRGIK
                     NLISIRIGASKTVRIERVRKAQNIDSRHALQIIESSDHDRAGFHKYFFSVDWYNPSEY
                     DMTLTTDRFDPIHAASAIDAFRMSFITEDREKEAQAKLKDLLLGAKIVTEITYNRKIP
                     IHFLEAAVDRGVVVLHGVSNSRATVDSAVATANLVPGVKQVESAIQLVQEFTVIP"
     gene            27867..28766
                     /locus_tag="PLS88_02665"
     CDS             27867..28766
                     /locus_tag="PLS88_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU06285.1"
                     /translation="MERFALLAAFGTAVCWAFSAIFFETASKKVGALAVNFWKVVFAF
                     LFLGISGMVERAVPFPYDAPIRTWILLGLSGLVGFVISDFFLFNAYILIGSRITVIFQ
                     SLTPIFTAFFAFIFIGERMQAYRLIGMAVTVAGILIVVTTRSRQNKKDNKEKLSAKGI
                     LYGFLSSIFQAGGMVLSKAGLGDYSPISGTQIRAFVAIFGFAINALLIGQGRFVLIEM
                     PKIKEAFRSTFKGSIFGPFLGVALSLFSMQHTQVGATSTLMALTPVVIIVPSVLILKQ
                     KVHYLEVVGAAVAVGGAALFFLL"
     gene            29047..30072
                     /gene="tdh"
                     /locus_tag="PLS88_02670"
     CDS             29047..30072
                     /gene="tdh"
                     /locus_tag="PLS88_02670"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOU06286.1"
                     /translation="MKALVKRNAEPGLWMEEVPIPHIGDNDLLIKVKKTAICGTDVHI
                     WKWNTWAQHTIHIGQIVGHEFVGEIVDKGRSVQDYRIGERVSAEGHIWCGTCRACRAG
                     KHHLCPNTIGIGVNRDGCFAEYVAVPAQNAWRVHEAISDDIAAIFDPFGNATHTALSF
                     DMTGEDVLITGAGPIGCMAAAIARHVGARNVVVTDVNPWRLELAKKLGATCVVNVDKE
                     DLKEVQKRLGMVGGFDVGMEMSGNPKALEQMIENMYNGGRMALLGLLPDGAGVDWSRI
                     IFKGLFIKGVYGREIFETWYKMQTMLLSGLDISPVITHRFSFDDFEKGFEAMLSGESG
                     KVILNLE"
     gene            30103..31539
                     /locus_tag="PLS88_02675"
     CDS             30103..31539
                     /locus_tag="PLS88_02675"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HOU06287.1"
                     /translation="MTENNLLYNFRISEDAASALERLPHCIDEANPISIQHRAELRYS
                     IQSLWRLLTMERAQRTSDYLNNPAFFSAYLRYFMPWNLVRLIALLTDLSVELKPDATI
                     IDMGSGPLTLPLSLYCAKPELRKLPLTIICADRAPRIMEAGKLILEILAVEHTGELPP
                     WKIELRHIRFGEPIKERADLFCAINVFNEFFWKGKGTLFENASDLYRSMIRYCSHDAS
                     MLIVEPGEPRCGGFLSALRAAAIQDNCEILAPCPHNSACPMPGIFKSGQEHIIERSPR
                     NLDIKKELGSGSILRSGFIRQSDSLRRSDSNQSKDNDFLAPVRMPAPRVKYPWCHFSM
                     PTSYAPKWLYRLSQEAGLAKEKLSFSYLYIKRYPSPMSSSNQNKEKKSRSPGKPPLLS
                     EANKGRDQIACRIVSEPIALSRSRVGRYACSPLGYTLISTAQSNSLPESGVLLRIQTR
                     SSAASDSTKKIEKETEYDQKTGAILISY"
     gene            31587..31660
                     /locus_tag="PLS88_02680"
     tRNA            31587..31660
                     /locus_tag="PLS88_02680"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31621..31623,aa:Asp,seq:gtc)
     gene            31775..31847
                     /locus_tag="PLS88_02685"
     tRNA            31775..31847
                     /locus_tag="PLS88_02685"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31808..31810,aa:Pro,seq:cgg)
     gene            complement(31971..32483)
                     /locus_tag="PLS88_02690"
     CDS             complement(31971..32483)
                     /locus_tag="PLS88_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06288.1"
                     /translation="MKKWWAITGLLLLISGAVWANNVTINATVLPADANILFDSYQWN
                     IPLVNDGATPYQNDQQGVMTVKSGSTWIFHIQFSSANLGYVKQGTDKIAYYVKVTELA
                     DSYGGIIMSSDIMNGYVQLTSSKTIQFIGKTSKNGARFAVGIKVNALAGQIYESGVYT
                     DTMTVTFFVF"
     gene            complement(32567..>32947)
                     /locus_tag="PLS88_02695"
     CDS             complement(32567..>32947)
                     /locus_tag="PLS88_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06289.1"
                     /translation="WSVTLDNTGTAVTVSPVDNLTVKSSKHAYTVTFSSSDSGQLKMT
                     SGTESYYIPYLIKVDTTGWGSGVSTNNLSDYVQLDTGKTIVFTARTPVAGKIFPISFK
                     INEYTDYYENGDYSATLTIGISAN"
BASE COUNT         9207 a         7560 c         7366 g         8714 t
ORIGIN      
        1 gactttttat ttaatgtgtt aaaattttat tgaggttcgg gaggatattc cgaatggaat
       61 ctggctgtaa gtcgaataat attacaaaaa taagagcgat aaagcttgta tttgttgctt
      121 ctttctgtgt tgcattattt tttgttattt ctaaaactgc aaggttatac gaaaccaaat
      181 taatatataa atcggataat aaatcatcga gtattttgtt ttctataaat cttggcaata
      241 tatatgatta tgatgatcct atttcaaagc tttattcaaa tccaaagaca aaagatgaag
      301 ttcttaaatt cttttctact atgactaatt cagaacgtgt tgccaaagct attttggata
      361 atgctgtggc tcacaatatg cggcctagcc ttgcttttgc tgtagcctat gtggaaagcc
      421 gatacaatcc gagagctcga tctaccaatc gaacgggcac actaaacagc ggccttttcc
      481 agcttaattc ttctgtatat ggcaaggtga acgaaaaact gatctacgat ccatatcaaa
      541 atgcaaagct cggaatagga catctccagg aatatatcaa tctaaatggg gatgaattct
      601 ctgctcttgc tgcatataac gcgggaataa ccaggatctc agaaaaaggt atacctgatg
      661 tgacttttaa atatatctcc gaagtatcta ttatggagca taaaattatg aacttcttta
      721 tcgctagcat tgctcttagc gaatcaatga agctttgatt cgttttgttc tctatttctt
      781 tcaaaagttg gacttatcat attatcgaca aaaggagaaa gcgcttcttg aagccctgca
      841 gcttcctttc ggccaagtac cacaagacga aatacagcag cctctacaag tgcaaaaaac
      901 atctcaactg tgccttttac cgaggatgat tccttgaact cgccgctctt tattccttca
      961 ataacaatat ctgcaaggat atgccgcatg cggacagttc ggcgccttac tttttcgtct
     1021 ggatttcctc cagaagcctt taatctcagc agataatcca ttactaccga gagcattttc
     1081 gattctctct cgcaggcttc aactaaaatt tcgcttatct ttagtatttt ttcgccgctt
     1141 gttaggcgct gatcttgggc aataagcgct atctgtgtct cgagagagga aagaaaacgc
     1201 tttatgcttt cgccaaagat ttgtttcttg ttgtcgaaat agagatatag gatcgttcta
     1261 gtgattccgc atctttcggc gatcttctgg aaagtcgtat ctgcataacc ttcttctata
     1321 aatacatcga gagctttttc tagaatctcc tgctttctct tttcatgggc gacttgagca
     1381 gacaaatcga cctcctttgt caaattgtta tgataacaga agatctaatc ttagtacatc
     1441 gtccgattaa attctctgtc cctcgcgttg cttgccacaa aaatacctag tatagttgat
     1501 ataggagctt atcgatggct ttgcagattc ttagaacttt gcgctctcaa aatattcctc
     1561 ctaagtacct ctggacgcct tcgggggcta tcgccctgcc cgatgcagta tcagcgcgct
     1621 ttgtagctca aaaatataag ctttctgcag gagagctgag agctctttct ttgatcgatg
     1681 aggcttttag gattatcatc gatctctata gaaagcaata ttcaaaactg cttgaagaaa
     1741 ttgcacttaa agcattcgct tccacagaag aaagcaaagc gctttgggaa gtgcttcaag
     1801 aattgattat agaattccct cctgccccca tctacgacgg ccttgcggag cccaaggatt
     1861 ggctggaatc gctgagcccc gttggagatg gctctaatag gcctaatcga gagctcataa
     1921 tagagcagct aatcctaatt cgtcttttta acgagaatcc ggcatgcagc ccatatcgat
     1981 ctctttttga tgacggagtc tctccaacag gcatcacatt gcctggctct atctctgcaa
     2041 agactcctta cttgcaggtt tttgctcgtc tcgaagatgc tttaaaaacc ttgcctggcc
     2101 tctcttatgg cgatgggaaa acccttgacc tcatcaattt tttgcgagag ccagcaagga
     2161 acgcacctac ttcgctcaag gctcagctcg aatggataat caagaattgg ggaacccttt
     2221 taggcgattt taggctcaat ttgcttgctg gaatcgacat gataaacgaa gagacaagat
     2281 cgcgttttcc acctggccca ggaccagccg tcccttatca gtatacaagc aacttgcatg
     2341 aatatgaaaa atttagcccc gacaagaatt ggatgccttc tcttgtgctt cttgcaaaaa
     2401 atgtccttgt ctggttacac cagctctcaa agatttactc aagagagata tcccgtctcg
     2461 atcagattcc cgaggaagag cttataatca tggccgaacg tgggataaat ggcctatggc
     2521 tcatcggaat atggcagcga agtccagcaa gcgagaagat aaaaaagctg tgtggaaact
     2581 ctgaagcagc tgcctctgct tactcgctct tcgactatga gatcagcccc gagctgggag
     2641 gctgggaggc actagatata ttccgcgaga gatgcggaca atatggcata aggcttgcag
     2701 ccgatatggt tcctaaccat acgggcatag attcgctctg gatgcgtaca agaccagagc
     2761 ttttcatgag tctctcctac tgtccgttcc ccagttatag cttcaatggt cccgatcttt
     2821 ccggagatcc atctattggg atttggctcg aagatcatta ttacaatcgc accgatgctg
     2881 ccgtggtatt caagagactc gatcgacata cgggagaagt tcgatacatt taccatggca
     2941 atgatgggac cggcatgcct tggaatgata ccgcacaaat agactttcta aaccatgctt
     3001 cgcgcgaggc tgtaaaagag cgaatcctgt atgttgcggc tcatttcaac ataatccgct
     3061 ttgacgcggc aatggtgctc gcaaaacagc atatcaggcg cttatggtac cccgcgccag
     3121 gctcgggagg agcaattcca tcgcgctcag agcatgccat aagcgatgag gcattcgata
     3181 aggcaatgcc aaatgaattc tggcgagagg tagtagatct atgcgcagaa aaagctccag
     3241 atacgcttct ccttgcggag gcattctggc ttatggaagg ctatttcgtg cgaacccttg
     3301 gtatgcaccg tgtatacaat tccgccttta tgaacatgct taaggacgag aagaattcac
     3361 tatatcgcct gactataaag aatacacagg aattcgatcg cgatatctta aagcgctttg
     3421 tcaattttat gagcaatcca gacgaggaga cggcagttgc tcaatttggc aagggagata
     3481 aatacttcgg tgttgcaacg atgcttgcca ccatgccagg actgccgatg attgcgcacg
     3541 gtcaaatcga aggttttaca gaaaagtacg gcatggaatt taagcgttct tattgggatg
     3601 aaacaccaga cagagatctt atcgcacgcc atgaacgcga gattttcccg ctgcttcgaa
     3661 tgcgcccgct tttctctgaa gtggaaaact tctatctgtt tgattatatg cgagacgatg
     3721 gaacaataga cgaaaatgtc tttgcctata gcaatggcca gggagagcag cgagttctag
     3781 tgttctataa taaccattgg gagcgtacat cggggcgaat tcacagctcc tctgcttttg
     3841 cccaaaagac cactgatggc aaaaaacacc taaaaacagt aagtctggcc agtgcgctca
     3901 agatcgattc ctcacctgga aattatgtta taatgcatga gattcgctct ggattatggt
     3961 atatattccg aagcgaagat attgcttccc atggtttcaa ggtagcgctg gaaggctacc
     4021 aaaacaaggt tttccttgat atcacaaatg tgcacgacac cgagggcaga tacacaaggc
     4081 tctttgaaat aattgactcg agaggaatag ccgacctgga cgatgccttg cttgaggctg
     4141 accaacctga gctctatcga tcgctccata atgcgatcag cagcctcagc agcatagaga
     4201 caattcaaaa tctgtcgaag gaagaagcta ttcaaagagc gaccattttt tcagagattt
     4261 tcttttcgag gctttgcgag attgcaggaa gcgacgatac tctcgctgcc tcccgaagcg
     4321 actcagtccg ggccgcaagc tcgtggctta aaatagtgct caaactgcta tatggaatgt
     4381 cggaatctga cagcggtaca agtactgccg cgacggttct tggccatggc ggtagtgcat
     4441 ccgattcgga atatcagcaa tatagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnngacg gttcttggcc atggcggtag tgcatccgat tcggaatatc agcaatatag
     4621 actgatcctc ttgatctatt cgttcatgag ggctttggca tgggctttcg ccgcagatgg
     4681 actcaatgag gaagcagctc gcatagtcaa agaatatcga atttcaaaaa aacttacaga
     4741 attagcagtc ggattgagtc gcagaagcga taatcgcgac gcagaatacc atgcctcaat
     4801 ttgcgcagaa atagcagtcg cttgggcact taggcaaaac agtcttcttt ttgacacaaa
     4861 aggtacaatc aattctattc aagctccaga caagcgtgct aatgaaatct gcgtatgggc
     4921 tttttctaat cctttaatgc gcgaagctct caatataaat caatatcgcg gaatagaata
     4981 ctttaataaa gagcgatttg aggcctttat aagtcttttg ccagcttttg catggatcga
     5041 ttcgattcaa gaagaaacca aggaaaaaga atggaaagaa tatctatcat ggaaagaagt
     5101 tgctgaaata ctgagagaga acgcaattgc cacaggctat aggacggcca caatgcttga
     5161 atatatggcc tctatcgcgc aagcatagag aaagggaaag ataacaagca tttttaggga
     5221 agattattcg atttatgcca aaatctgtag attcgactat aatagcggag caatatcgat
     5281 ctctttttga aacaccgctt ggtcgcgata ttgtgcaaaa cctgcaagac gagaatgtcg
     5341 tttttgtttt tccttcacag gacaccgccg attcatgggc tcaagctatt gtaaaatctg
     5401 aaataaccag agcagtggct ctcgatcgtt ttttgggttt cgagaatttc ctgctatatg
     5461 tggcaaaggc taatgaacat gatgatctat gtgcactaaa gcctatcgac cgctggatct
     5521 gggcacttca aatgctttca aaagaacata atataggaga taaaaggcct aataaaggat
     5581 tgtctgcttt gcttccttcg cttcctacga tggaaatatc gaccaagaat cttgcaaggc
     5641 tcgtgcgact cgtcccctct ctattcgaaa tcgaaagcct gacaaaaggt agcaccgcgc
     5701 tcgatatatc ttcatatcgc ttcatagaag aagaattgga ggcaatagac ttactcgctg
     5761 aagactactt cgattttctt gatcgttcta aacttatcga tgcgcatagc tgccctcttg
     5821 acttgccaaa gtccatgaaa gttcgagctt atgggctcaa agatgatttt atccgatttg
     5881 ggcttataga tacaaaagag ccgagcgaca gaaatattgg ttgcaatgga atcttcagcg
     5941 aaaaagcaag cactaaagaa tccctcaagg ctaacagcca gattgttttc ccagacatca
     6001 tcactcaaga aagagcgata aagacttcta ttctttaccg cgaattcagc tcctcgcttg
     6061 atgaattcga atggatactg gaagaaatat cgaacgaaat cgaacaaggt atcgagcctg
     6121 aagatattgc tatatcggta tgtgggctaa atgcgaaaaa ggcagcttgg attcgtcaga
     6181 ttgccactga atatggtgtt ccaatctcta ttcgccgagg caagccacta tccatatccc
     6241 cctttggaag actattggtt gcgattcaaa acgcggctcg ggaaggctta actctggaaa
     6301 gcctcgatgc atttgcggaa cttgccaata tcacaaaacg cgatccagaa ggctggaaag
     6361 cgctgcgaga tacagcgctg catctacatc tgccttcgcc atctccaaat gccgaatata
     6421 tacacagtct atggaaagaa gctgttaaga caggccaatg tcccgaatcg acggctcaga
     6481 tgtatcaaag gctttgggaa agcattttgt ttatttccaa ggctagctca ttctccaagc
     6541 tttatgatta tatactcggt tttctcgagc gttgggttga tacaagcgga ttcggagcca
     6601 atatccagac agatcgctca atgcgccttg ccctagatga actccagggt tggatagacc
     6661 atgaatacat ttctccaaac ctctctattc cgccatttga gctcttcatt gctgtgctgg
     6721 aatcaaagag ctatatacct ctcgtagaag agaatgcagt acatgtgtac gacttcgata
     6781 caaccgcagg aattgccgca ctttcccatt atattgcagg agcctcgcag gaaggtttgg
     6841 ctccttcact caaactcaga agcgctcttc caccggagct tgctggccta atccaccagg
     6901 agaagacaga gaaggtttat gaagccataa atctccatca agtctcgccg actcagttct
     6961 cctttgcgac cgaaagtttt gaagattttg aggcagccta tccactattc cctcctccag
     7021 taaagactgc ctcccataca aagcataggc ttcgacctat cttggttttt gaagaacaaa
     7081 aaactgctac aaagaactat tctcatccaa aacctatcga gaccgcaata gcacgtcgtt
     7141 ttctagatga aactaaaagt atagatttag aaaagaatta ttctattttt agcccttctt
     7201 cgctagaaga tcgatcgcgg tgtgggttcc gctggtttgc cagcaggatg aggctaagcg
     7261 atgtctatgc tagagacgat agcgccatag ctatcggtaa cttttatcat cgcgcatata
     7321 gactcgctat ccaaagactt ctgcgaaacc ataaagatgg ccaagctgag gatgaatttc
     7381 agagagctct aaactcagcc ctgcgcatcg aagcagagag aattctcctc gagtatggca
     7441 atggacttcg ccctatccta tcggctcttg taccgcgtgc aagccataga ctccatgaac
     7501 tttggaattt cgagcagaag gtgttttcag cttatgagcg cgaagggttt gaagtagagc
     7561 ttcggaatga atttacagat gaagaagcaa ttctcggagg aagaatcgac tgcctcttta
     7621 gcaggcaaga tgaaagcctt ggcaatataa aatgttatgt aattgtggat tataaaaaga
     7681 acaccatacc atccatatct aaaatgaaac ttagtgccat gccctcggct ttggacgaac
     7741 tagtatcttc tgatgaaact caagaggaag aagtaaacgg atataacgat agcgcgcttt
     7801 tactccaaga aatccagata ccttcttatg ctcttttgtt ggagacttcc gggggccgta
     7861 tagacggggc tttttattgg tcgatcgaaa aagcggaagc tctcggatat atcaagcctc
     7921 caacagtgcc taagatgaaa tcagcatata cttacagtaa tgaaacggga gatgtccgct
     7981 ccacgcttcg cgacatgttg cgaaccgcct cttcggcagt aaaagccgga aattttctgg
     8041 atcctgcgct cgatcgggca gtctgtgaag actgccaatt caaacccctt tgcagatact
     8101 ggtattttct ggagttataa tgaaaccgca gcaagattca ttcgacctcg aagcgctcaa
     8161 gctcgacgat tttcaaaaaa gggccgcata taccgaagat acagccatcg ttacagcggg
     8221 agcgggagca ggcaaaacaa gagcattggt aggtcgtttt ctatatcttg tgcttcagaa
     8281 aaatgtagat ccagagcgta tcttggccct tacatttact cgcaaggcag cggcagaaat
     8341 gttccagcga gtccaactga ctctgagccc atttgcaaat gatagtccag ctcttgctaa
     8401 aagccttgtg caagcgcata tccagacgct cgactctttc tgccgcgaaa tcgtggctca
     8461 ggctgccacc ttatatggct atactccaga ctttaaaata gacgagagca agtgcgctcg
     8521 tattgcagaa aagaccgcat accgatatgt cctcgacaat cagaaagaaa atgggctaga
     8581 aagcctcgtt gccacctttg gttttgaagc cgtggttcag gacctttttg cctctttcgg
     8641 aaaggcaaat gtcgaaccga attggcaagg caagcaaatc tgtcgcagct cggctcagat
     8701 tggagaacaa gaattcagga aaatagctgc agaaatatca aaaaaaatac atgatatcgc
     8761 gctcaatata ctggaaatcg ctcaccgaga accaaaccat gatttcaaag aaggaactaa
     8821 tcaggctatt cgcatggcaa aagcggtctc tagcttttct tgtgatctta caggtttgag
     8881 agactttctc gattacatgg aaaagccaga aaatgccctt aatatgcgaa gcataggccg
     8941 aaatgaaaca gaatccgcta tcaaggagct tgccaaagag cttaaagata aatcgtttca
     9001 ggataaaatc tctcaagttt tagatttcga ggagtttttg ccgacctatt acgctataat
     9061 gaatcggctc gatgaatacg ccgacatgct atgtgaagaa aagtgcatag ctaatatcat
     9121 gaattacaaa gatctaggcg cattagcagt agacattctg tccaagcatg aagacaggcg
     9181 cattttttgg gcttctcgtt ttcagtatat cctcatcgat gaattccagg ataacaacga
     9241 gcttcagaag caactccttt ttctcttaag caaagaaggt gatgagattc gcagaggaaa
     9301 attgtttttt gtcggagatg agaaacaatc aatttacctc ttccgtggtg caaatgtaag
     9361 tgtattcaag ggtctagccg atgagctaat ggcagttcct ctttcgctaa ccagaaacta
     9421 ccgcagcaca agccaactaa tcgaattctt caacgagctt ttccgcatcg ttatgcaacc
     9481 agccaaccca gaaaatccac aacctttcga ggctcgatat gaggacatgg aaacaagaag
     9541 catagcagat tctaataaag aagaacctca aggcttttcc tcccgtatag aatatcattg
     9601 cataactagc aaaacaccac tagaagaagc tggcagctta tattccgtaa gggagtcaat
     9661 ggcttttagg ctagctctgt ggatacgcaa cgctgtcgaa tcttctaatc ctctttttat
     9721 ccgaaatgaa aagcaaacgc aggatcctgc agcaagagaa gcaaatcact ctgaaacatc
     9781 gcttagaaag gccgaataca gcgatatcgc tgtcctcatg cgcacaacaa gtcgtcaata
     9841 cgaaatcgaa aaatatctac gtatgctagc cattcccttt gtaagcgata ccgccgccag
     9901 cctcttctct gaagagccga taaatgacct ttactacctt ttacgtcttc tcgtagatgc
     9961 tgatgatctt tttgccacag ccgcagtgct ccgtagcccc ttatgtcgaa tttctaacga
    10021 tggatttgta agcatcctca ctgagaaaaa gagcctggcc gatctcgagg ctggtatacc
    10081 aaaagaactg aatcccaatg ataaaaattc agtagaacga atgatttcat tctatcggga
    10141 attgcgtcca atgtcagatc acatgcccct catggagctt atcgcctata tatggaagaa
    10201 tgcgcagctc gaggcttcga ttctctgcga cgaacaacgc ctgccatact tagagcattg
    10261 gaatgccata aggaccatag cagcaagcat agaggcagaa ggtgggcata tagccgcgtt
    10321 tctaatatca atccgagatt atatgcaaaa tatgcatatg ttcgacacaa gttccgttcc
    10381 aaaggcagaa gcgcatggtg tccatttgct cactatccat aaatctaaag gactcgagtt
    10441 cccaatagtg atcattccat ggactgaagc aagcactaac agaaatagcg gaaaagagct
    10501 ctggggaatt ctcgagtcgg atcaagagca aggaaaaaca caattctata cagtggacat
    10561 aggatttcac gatcgaatca agagtggctc caatattctt cagactcagg caaaagccct
    10621 tcgcacagag aaagagcaag cggaaaccaa gcgcctttta tatgtagctt gcacaagagc
    10681 aatcgatcac cttgcattct tctgtgcggc tcctgagaag agatcctacg ataaagacag
    10741 cttccatgcg cttcttttca aggacaaatc tcatctcctc cagcaagaca gcgatgataa
    10801 aactcaattg ccttctcatc tatcggattt gcggctattt ttcgaaccgc tagtctctga
    10861 caaagaaata caaagagtca gatcacatgg gttaattgaa aggaaattgt ctcaaaaaga
    10921 gctcctctct caagaagaag cacaaaaatt agcaccgaca tccaaagaaa catcatgcaa
    10981 aattgcctca aaaaaagaag aagttttagc cgaacatgct attatgacaa atctgacttc
    11041 taactctgtt gccaaccatc attctcgaca tatcacaatt agcagcataa acgctataat
    11101 ttcaaaggag actcttgctg cggactatgc gcgcgagcta tccaagcatt tcgtaagaga
    11161 acatatattc attcctttag aacatcaggc cttgtcggaa accaaagccc cagataaagt
    11221 tctccttgag gtaaaaaatt cctgggaatc tcttaactct agcgcctatg ggtcgttggt
    11281 ccatgaactc ttcgcccatc ttttggctgg aaagtccatt gaaagctata ggccatccgt
    11341 ttccatatcg cgccaactac ttgggaatac gtcagccaca gatatcgatg tttccaacac
    11401 caataaagct acccatcgtc atatgaggtt tctcatagaa aatgctgcgc gcgagcttgc
    11461 tcctttcttt gaagccccta gattctcaac tctaatcctg gaaaagaaat atagctgcga
    11521 attccctttc ctccttggac ttggacgatg tatcatcgag ggaagaatgg acttcatagc
    11581 ccaaagagat gaagaagtca taatacttga cttgaaaagc gatcagagat tctcgcctta
    11641 cgaatattct ctccagctag ctctttatat tatggcagct cggcaactct ttcaaaagaa
    11701 aaaagtacgc gcaggtctta tgtatctgca ttttggggaa attgcctggc tagaaaccga
    11761 gcctgaggag aaaaccatat tgcggatctg cgattcaata tcctttgaca agcataacaa
    11821 ttagtaatcc aggaggatcg atgaaaatac cctctaaggg aaaaggccca aataaaacca
    11881 taaaaaagac tgagccaacc cctttgagaa tctttcttac tttggcccag atcaatacac
    11941 ttacgcttgg tggcggatat gtgattgttc ctgtaatagg caattccttc cagaaacagg
    12001 gttggatcga cgaagaagaa ttctatagga ttttttcgcg tgcccaggtg tttccaggcc
    12061 ctgtagcact cagtacttcc tttctctgtt cttatagaat tgcgggcgtc aaaggagcta
    12121 ttgctgcggt tctcggagta gtgctgccgc cttttatagc acttatcctt gttggaggct
    12181 ttatcagcct ttatggagat tccgagcttt tcaagcgctt tttgaatggc gctggcgctg
    12241 tagtgccggg ccttgtcggc tcgatgctct ggaagactgc aaaaaatcga aaatggacgg
    12301 ctagagtttt catcgaagta ggaatattgg ctatattgct aattcttttc ccatcggctg
    12361 ctttctttat tttgataggc ggaattgtag ctctatacct aggaaggctt ttatggaaat
    12421 cctgaaaata tcccttgcct ttttcaagat aggcatagtc gcatttggag gaggctggtc
    12481 agttgtaggc ctcatcaaac aagagatagt cccaaactgg atgagcgccg atgcttttag
    12541 ctcactcatc gcgattgctc agtccacgcc aggacctatc gcactcaatg cagctacact
    12601 ggtaggttgg caaaaggctg gttttcttgg tgcactagct gcgaccttct cagtcatagc
    12661 tttcccgctt ctagcgatgt ccattacgat ggctttttcc aaatatatac ctttaaaaaa
    12721 ggatctagcc gatgagagct tgcgatcagg cagcatggcg atgatcatta tgacactctg
    12781 gactctgttg cctcagaata ccctacccgg gccatggata ttcgctctgg catcctttgg
    12841 cgttacagct tttaccaaag taaatggtgt atgggtcatt cttggggcag gagctatcaa
    12901 tatgctctta ggtgtgcttc cttgagcttg ggctatagtt ttaaaattta tagttagtcg
    12961 aagctattct ttcaatcttc gtcgcacgca tggacagttc ctggcccaca ttctgcagga
    13021 ggctctacgg gcgcagggac gacattcgcc agttcaggac gctcgacagt gtatttttta
    13081 acaaaaccta ctggtctata agtcattttt gcttgcctaa ggcccagatc tcccaggtcc
    13141 tgctcgcgat ttatctgcag ataatgcttg ggcagagatt gggcaaaggc ttgattgatg
    13201 aactgataga tgcctttata gctgtcgatc gctttttcga aatggatggc gaacatctta
    13261 ccttttgcaa gtggctcgcc aaggcaccaa cctactggcc tattgtcgat ataatagacc
    13321 gctcctcgca agcccaagat atcgaagcac tccaaggcct cgcgagcagc cgcatagtct
    13381 ccctctgccc cttttgccgc tctccactcc tcaagcacta acagtgcatc cgtggttcgg
    13441 tctttagtca aaggtcgctg ttcgcaggcg taattgctga caaagccatt aactaagttg
    13501 cgcttcttgt gatatatacg gccattgagg gtggctaagt cttctcgatt atagagataa
    13561 tcgaaattat ccctgtcctc tcgtactaaa tatccacgcc tttcaagctc gattcttttt
    13621 tttgacgctt ggcattccga gagatggcgc atatagtcga aatgtagcat aagctcatcg
    13681 aattgctcca tcgatggaaa gcagcaggga atatagaaga atgtcctgcc cttatagcga
    13741 cctgatatga tgaatttttc gtccgttagt tgtgaaatct cataaccata ggtatttcgg
    13801 aaaagatata gccctgaaaa ggtgaattcc gagatgccat caggcagcag atttagagct
    13861 gggtagagaa tatctcttgc atcgagcgta aggggagcaa attctggata tgttggaatt
    13921 accatagaat ttcataatac tccgcaacaa gacccaagac aagaatccca gactgagcta
    13981 tccaaggttg aatagataca gtggtaaaaa atgcgatatt tgcaccttaa tttatgagga
    14041 ctcccttgcg caaaccatca tccagatgtc atattgaact atcatgatag caatactcga
    14101 ttatctaaac cgttcctaca cggaagaaat gttgaatcgt tttctcagct atgtaaaaat
    14161 tgatactcag agcaaccgcc atgctgataa aacaccaacg accgaaggtc agtgggatct
    14221 tgcgcacaag ctcgaacaag agctgctaga gctcggcatt tcagagctag agctcgatag
    14281 caattgttac ctgatagctc atctacctgc gagcccaggc aaggaacatg tgccatgcat
    14341 tggccttatg gctcacatgg acactgcaag cgatgtttca ggaagcggag tacagccgcg
    14401 tgtcatcaag aattacgatg gtaaggcaat aagtctctcc aagcagtata tgctagatcc
    14461 caacgaattc cccgatctct gtgagcatgt gggggatacc attgtggtaa cagatggctc
    14521 cactcttctt ggagcagatg ataaagctgg cgttgcagaa atcatgaccg ccattgctta
    14581 tcttttacag catcaagagc ttaaacatgg gcctatcgat atatatttca ctccagacga
    14641 agaaacgggc aagggaatgg atctcttccc tttggagaaa gctcgagcca aggcttgcta
    14701 cacactcgat ggtggtgcag cacccgaagt ggaggccgaa tgtttcaatg cttatgttgt
    14761 aaaggctgat ttctttggca aagtaattca tatcggcgct gctcgcggaa aactcgccaa
    14821 tgccgttgcc atggcaggaa gctttatcga catgttgcca cgttcggaga gcccagaagc
    14881 caccgatgga tggtatggct attactgccc cgtcgaagtc tccggagggc tcgaacacgc
    14941 atgggcggaa gtataccttc gagatttttc agcttcccgt atgcaggaga gaatagccgc
    15001 actcaaagct tttgctaaag ccgtagaagc acagttccct caaggccaca tcgaactaag
    15061 cgttacccaa cagtatctca atatgaagga aaaactcgtt aaccatcccg aggtcctcga
    15121 aaaactcaaa aaagccataa aggatcaaaa tatcgagccc ataatgaagc caatccgtgg
    15181 tggcaccgat ggctcgcgct taaccgaaat gggaatccca actccaaacc tctttacagg
    15241 tggctacaat taccatagcc gcttcgaatg ggcttctctc tcagatatgg cgctctcggt
    15301 tgagactttg cttaaccttg tacaagtatg ggctgactag gataaaagca taaaggccga
    15361 agaataagcg taatcagcgc tattgcttag atattataaa tattccttaa aatctgtaac
    15421 tttctgactt ttatgcttta ttttatttaa ggataagatg cgctcattcg agaaaaatta
    15481 ccctttgtgg ataaaatgag actcatttaa ttatatactg ctgtataaat atgggtttac
    15541 acaaatgcac tcatatctta tagttattcg ccaacatttg gaaaaaggca gtagaaaaaa
    15601 ggtaggttaa tgttttctcc tttggatttt aaggaatatg agcaggttgc aagcaactct
    15661 ggagcattta agggcgtcga aatcgatctt atgctcgaaa tcctcaaaga ttggcagcaa
    15721 gcgccaggca agagttatac cctcctagaa ctgcgcgatg gaaagactct tgtcgctttt
    15781 gcgataatcc ataaggtagc agggcgaaac tttacattcg atattcgttt tgtcgtacta
    15841 gatagcgatt atcgatccac aaacgcaatc caacaccttt tcgaaatgat cgatcaagaa
    15901 ctccttaaaa aaatgccttt tgcggtgatt agatttgaat tatcttccga taaaagagat
    15961 agcttgggag aaaatacgct cgaaaattca ggctattgtc aaataggaca tattacagaa
    16021 ttctatggcg caaatgacga ttttttcttc tatataaagg ctatttttag aaatcctcca
    16081 aatttcatca aaatatcgaa acctttcgag gatgaattat ccccggaatc cttgcccgaa
    16141 gaaactaata atcagcaata ggatcaaaat atgatcgtag gtgcaatcga aggcggtgga
    16201 acgaaattcg tctgtgccgt tctaaaattc gatgattcaa atattcaaga aaagccccct
    16261 cgaatcttaa aaatcgaacg aatcctgact acagatccag agtcgaccct cgatgcggtt
    16321 gtccgtttct tcaaaaattc tgggattgaa tgcgctggtc ttggaatgtt tggcccaata
    16381 gaatgcagaa tcgagagccc gagatggggt tatctgctaa atacgccgaa accaggctgg
    16441 agcgatatag acatagctca aaggttgcag aaacaattac aagtccctct caaactcgaa
    16501 accgatgtgg ccgcttctgg cattggcgaa tggaaatggg gagcagggcg aggaacgcat
    16561 tgctgcgtat atatcacaat aggaacagga attggagcag gtattatcat cgatggtatt
    16621 cctgtacgcg catccttcca tcccgagatg gggcatatgc gggtcccaag agcaagaact
    16681 atagaatcca gactcgacga gttcgagggc atctgcatct atcatagcga ttgtttggaa
    16741 gggctcgcca gcggaccagc aatagccgct cgctggagag aggcacccga aaacttgccg
    16801 acagaacacc ctgcctggcg gctcgaagct gaataccttg catttgcgct caccaatatt
    16861 gcgctcacta tagcacctga tcgcatcatc gttgggggtg gaattggtct tcgtgaagga
    16921 ttgctcgata tggttggcaa gcgcatgagg gagcttttgg gtgggtatat ccaagccttg
    16981 agcagcgcgg aagcaataag actttttcta gcccgagcaa agcttggcaa cgaggctggc
    17041 gtactcggag cggccgaact ggctagacaa ctcttcaata gtgtctcgaa acgttgaaac
    17101 accttcaaaa aaaacaagtc atcagaaaat aatccggaga taaatcctaa gcgcagaaaa
    17161 tcgatataat aatgaacctt accttgattt ttgtggcctg attctgctat agtacgctcg
    17221 ttctaaccaa gccgagatag ctcatctggc agagcggcgc actcgtaatg cgcaggtgtc
    17281 gggttcgatt cccgatctcg gctatgatgc tgtagcatga attccattgt tcatagattc
    17341 ggcagagccg tttcgagcca ggtacctcaa aatcctcagc tttctcgtaa tattttggct
    17401 gcaggctata cgctcagtgg ctggaaggat gcatatttcg gtggtaaagg cctaaaaggg
    17461 gtgtatgctc acatgaatgc cgcaataagc agaaacatga gccactcttt tagagattca
    17521 tcgcgtagtg ccatggtcaa tatcttcttc ccatgcgaga ttctatatgc aatggatatt
    17581 gcgcccgtgt ttccggaagc attgtcagta tacattgtaa attcagactg tggagaggtc
    17641 tttgcagaat cagcggaagc tatcggcata tctgaaagct tgtgctctta tcataaaatc
    17701 atgatcggta cagctaggac aggagtactt cctaagccac tgcttatagc caatactact
    17761 ttggcctgcg atgctaatca gctatccttc aggtaccttg ccgaagaata tggcagccct
    17821 catttcatag tcgacgtgcc tcatacagta aacaaggagt ctgtacatta tgtggcttcc
    17881 caactcaaaa atttcactgt tcttctagag gatctcttgc acagttctat gtctgaatcg
    17941 aagcttcgat ataccgtcga tagggccaat cgcacgatca aggcctaccg agaatacttg
    18001 aacattcgcg caaggacttc gctccccatc tccatgactg gagagctgtt ttctcttgtt
    18061 gcgacgcatg tgttcctggg aacacccgag agcgagcaat atgccattgc aataaaagaa
    18121 ttagcgcaga aagcttctcc agatcgaagg aaaaaacgca ttctttggct gcacactctg
    18181 ccaaattggc aggattctat cagagttctt cttgaaaaca acgagcgcac cgagatccta
    18241 gcaagcgata tgacttatga tacactcata gatatggatc cagaaaagcc atacgaaagc
    18301 atggccgaac gccttgtata caatatcaac aacggaggtg cgcagagacg aatcgacgca
    18361 gccatagcct atgcaaaaaa gttgcacgcc gatggcgtaa ttgtgtttgg ccagtgggga
    18421 tgcaagcaaa caatgggcat gtctcaatta gcaaaaatgg ctctcgaaga ggagggtttc
    18481 ccaaccctag tgctcgatgg agacggctgc gatccacgca atgtggctga tggtcaaatg
    18541 gcgactcggg tcaatgcttt tctggaacag ctagaggcat cgcgatgatt ggctacactt
    18601 gcaaatacac gcctatcgag ctcttatatg ctttaggaga gaccacagag ctgctcaacc
    18661 atgaagttac ggatttcgaa tatgccgaga aatggactca tccaaacctt tgctgccatg
    18721 caaaggcctt cttgctgcat agctctgagc tgaacgagct tgtcttgata aactgttgcg
    18781 attccattcg tagagcatac gatgtgttgc tcgcgcagaa atcccatcga tttctatttt
    18841 tgttcgacct tccacacata gatgatggct gcggacaaga aaatgtcaaa aaagagctgg
    18901 tccgcctgac tcaacgctac atagaatatc atcctgtctc ctttgatcga caacgattcc
    18961 ttaaagcatg cagggcatct tctgtttctt cttttgatta tgcatctgat gctttctttg
    19021 aggctccatc tgaggctact tctaaactta aagaacttga ttctgcggcc acattcatcg
    19081 cgatagtagg ggcgcgagta agtcaagaac ttctttcgac aatcaaagaa aaagtgccct
    19141 atcccgtgat cgatgccaca tgtggcggaa accgacatct cggcttattg cctccgaatg
    19201 ctgaaaatat aagcttcgat gagctcatgg attggtatgc aaaagagttg ttgcatttta
    19261 taccatgtat gcgtatggca aatatcgagc ctcgcagggc gctttgggaa aatgagaatc
    19321 tctgtggaat aatctataat accgtcaaat tttgcgacta ttatagtttt gattattccc
    19381 gagtaaaaaa agaagcaagt ctacctatct tgaagatcga atccgatttt acgcctcaaa
    19441 ccgaaggcca gctctctacg cgtctcgaag ctttccttga aaatattcaa gctcgacaaa
    19501 aaaagccaca gaaacctaaa aagcaacagc taaggcattc ggaccttttt ggaggaatcg
    19561 atagcggttc caccacgaca aagatggttg tcatcgatcg aaataggaaa attctcgctt
    19621 cgagcttagt atccaccggt gcccaaacac agaaaagcgc gcagaaagcg ctgcaggaaa
    19681 catgtcggca gctcgatata gaaccggtcg acttggcatc tattgtctcg acaggctatg
    19741 ggcgaaacaa tattgcattt gcaacagaca gtaaaaccga gataacctgt cacgcaaaag
    19801 gcgcgttttt tcttgaccct actattcgca ccatagtgga tataggcgga caagatagca
    19861 aaattatccg catagacaat caaggcagcg ttgtcaattt cgtcatgaat gacaaatgcg
    19921 cagccggcac agggcgcttc ctcgagatga tggctcgcgc cctcgagcta gatatcgaca
    19981 atatggtccg cactggccta gcgtggaaaa gcgaccttac gatctctagc atgtgcacgg
    20041 tttttgctga atccgaagtc gtctcgctca tcgcagacaa tcgctctatt gcagacatag
    20101 tacatgggct caacaaatcg atcgcttcga gaataacttc gatgataagc agagctggag
    20161 gccaagcgcc atatatgatg acaggaggcg tagcccgaaa caccggtgta gttagagaac
    20221 tcgaaataaa gctcggcgaa cgactcatta tctctgaaat tccggagctc tgcggtgctc
    20281 ttggcgccgc gctatctgcc ctggaattga tggaaggata ggcaatctct ttgcaataaa
    20341 tgaatatggc aataagggag gaaaagaaca tggaaaaagc aaaagtgggc ataattggcg
    20401 gatcaggcct ttatgaaatc cagggagcaa aggtagtcga agaaatcaaa atggatactc
    20461 cttggggatc tccctccgac gacatcacca ttgcggagat tgaaggtgtc aagacagcct
    20521 tcctgcctcg ccatggaaaa gggcattttc ttttgcccac cgaagtcaat tcgagagcca
    20581 atatcgcggc cctaaaaatg cttggtgtcc gtgagatcat agcattctct gcggttggga
    20641 gcctcaagga ggatattcat ccacgcgatt tcgtattgcc ctctcaaatc atagatagaa
    20701 ccaagggcag gtcaggctcc agctttttcg gagaaggtat tgtcgctcat ttgagctttg
    20761 cggatccctt ttgcatgcgg ctttgttcga taatagagcc tttcatttta gcgcgaggat
    20821 tgcgtctaca taccaatgaa accctaatct gtatggaagg gcctgctttc tcgacaaggg
    20881 cagagagcca gctttaccgt tcctggggcg ctggtattat caatatgagc gtgctgccag
    20941 aggcaaaatt ggctcgcgaa gccgagatct gttatgcaat ggtctgtatg gcgacagatt
    21001 acgactgctg gaaaacagac cacgcagatg tgacggtcga tatggtcgta caaaacctca
    21061 atgcaaactc agctcatgcc aaggcattga tcctggaagt ggttcctgcc ttaagtaagg
    21121 atgccgaatg cagctgtcat accgccatga ggggtgccat tatgactgcc ccatcaaagc
    21181 gcaatccaga aaccatggca aaactccgaa caatactccc tgagcttgag ctctagagtc
    21241 tatgggcaac tagatgtaag cagcccagct tgaccctatt agccttgcca attactctgt
    21301 cctcatgaaa atcttgatag ccaacgatca tggcgctgta gcgctaaaat tccatctctt
    21361 gcgctggctc gaaagccagg ggcaccaagt tgtcaatctc ggtgtcgata ccgaagatcg
    21421 tgtcgattat ccagatcagg cagccctggc cgtaaaagaa tattttgggg gcagttacga
    21481 tttgggcata gtatgctgtg gtacaggcat tggcatatcg attgccgcca atagattcaa
    21541 aggtattcgc tgcgcactta ttcatgatag ctataccgct cgcatggcaa aagagcacaa
    21601 taatgccaat ttcctcgctt ttggcggccg tgtggattac aaagagccca tcgaggaact
    21661 cttgagtgtc ttcttgcatg cagaattcca gggagggcgc cacgcagaaa gagtctcaaa
    21721 acttgataag atttaggata tcttgtagat aacacccatt ttcgcaatct cgatatcctt
    21781 taggccagca tcttgcagat ggccctgcat tgccatgagt gcctctggtt cgccatgtac
    21841 aagaaaggtc ccctttaggc gctctttttc gatacaatcg agccattcta cagtctcttt
    21901 ccaatcggca tgcgcagaaa aggcatcgat ttcttcgata tttgcgcgaa cttgaaacca
    21961 atcgctctga atacgcacct cttttgcgcc atctctgagt cgtctgccta atgttccctc
    22021 agccatgtat cccacgatga gcacggtatt cgcagaatca ccgagtccat gcatcagatg
    22081 atgttgaatc cgtccaaatt cgcacatgcc atcggcagaa atgattatga gcggaccctt
    22141 agcttgattg agagccttgg aatcctccac ggatcgggaa aaattgaggg ccgaaaaacc
    22201 aaagggattc ttggcatggg tcgtaaaaag atcgtaagtc tccttatcgt agcattctgg
    22261 atgtattgca aagatacttg tcgcattgag agccatgggc gaatccaccc atatcggaat
    22321 atcgggtatc ctaccctgat cgtgcaaaag atgaaggcaa aagatcagct cctgtgtgcg
    22381 ctccactgca aaagctggaa tgatgagctt gcctcgcctt tcagcagtcc tattaacgac
    22441 atcttcgagg tgtcgaagag catccattgt ggattcatga agacgatttc cataggtgct
    22501 atctagaatg agaaaatcta ttttatcgag gcattcggga tccttgatta ttggcttgcc
    22561 tttgcgcccc aaatctccgg aaaaaccaag aatagctaca ttcccagcct tgtcctttgc
    22621 tgtaattcgc gcaaaagtcg atcctagaat atgacctgca tcgagaaatt ctacttgtat
    22681 tccatcagct atagatatag gcctatgata gctgaccgtt acaaactgat ccatcgcgcg
    22741 tataacgtcg aagtcgtcat acaaaggctt ccagtcgaat ttctcgtgcc ttttctccgc
    22801 ctgtctggaa agataatctg catctctcgc ttgaatgtgt gcggaatcgc tcataatcaa
    22861 gtttgcgaga tctctcgtgg ccgatgttgc gtagatgttg ccagtgaaac ccttttttgc
    22921 caaatagggc acaagcccgc aatgatcgta atgcgcatga gtaagcacta aggcatcgat
    22981 agatgaagcc tctatatcat ctccaagcaa agccctgttc ttgttatccg ctttagctct
    23041 tttcccttga aaagctccac agtctatgag aatccttgta gaatctactt ctagaaggtg
    23101 cttcgaacca gtaacctcct cagccgctcc aagtgaacaa atactgatgc tcatgccttc
    23161 actataacct gttgcgaaaa agtccgcaat tcgatacatt agtacaggct catgtgcgct
    23221 gatacgctca cccttagggt tgaaaaactc tcttcaaacg gtgagggtat cgctttttca
    23281 gaaggaaaag cagttttctt gcctttcgca attccaggcg agacagcgct tgcagaaata
    23341 atcgaagaac attctagctt ttcgagagca agactcatcg atatagaaga atcctcccct
    23401 tatcgggtgg aacctttttg ccctcatttt ggcgactgcg gcggatgtac tcttcagcat
    23461 attgggtatg aataccaagc attgctcaaa aaacaagcgg ctcaagggac ctttatccgt
    23521 atcggaggct ttgatcccgg agagcttcaa atcatcacag gagaaagcta ccattatcgc
    23581 aatcgcgccc agatccatgc ctgtggagat ggaggcctcg gttttgcaaa gactggctca
    23641 cacgagagcg taagggcatc tcattgtcct gccctcgttc caattatcga gcgctggctg
    23701 atctcggaaa atcgaaaagc ccatccctat cgcgccctgt ccgccctgat cggcgacaga
    23761 cctcgctttg tagtttttgg ccaggaggat cgactgtaca ttgaaggaag agacggcttt
    23821 ggaaaggcaa aagttgccgg caaggaatta aagttcccgc ttgcaagctt ttttcagagc
    23881 aatatctcaa tgctcgaaaa gctaattgaa cgggaagtct caccgttgaa gggtaagcga
    23941 gctctcgacc tctattgcgg cgccggcctt ttttcgctat tcctggcgga aagattcgaa
    24001 gcgatagatt gtgtcgaaag cgaagctgct tctttggaag ctgctcgaat gaacctcgaa
    24061 tcgacaaagg caaagatcga gttttcgtgc accacggtgg agcattggac agaaaaaact
    24121 ccgaccgacc tatccttcga ttatatcgtt gccgacccac cgcgaactgg aatggcaccg
    24181 caggtgcgca cgtggctttc ttttgtggat gctccagcac ttctctatat cagctgcgat
    24241 cttgcaagcc ttgcacgcga tctgcgagac ctctttcaaa aaggctggca gctcgaaaat
    24301 atgacacttt atgacttcta tcctcagacg ggaaggctcg aagcggccgt ccatctaaaa
    24361 aaggaggcta aacatgacct gtaatggtcc tttcaaaggc agatctattt cggtcgtaaa
    24421 cgatctatcg ctggatgaac aactctatct ataccgcaag gctcgagaat tgaaagaaac
    24481 cgcgcgagct ggtgacgatg tagagaagtt ccggatcaat gataaggatt atcaggtgta
    24541 cttgattttt atggaaaact ctacacggac ccgcgaatcc tttagaaatg caggcaaatt
    24601 cctgggtgct cgggtcaatg tcttcgacgc cgcaacaagc tccttcaata agaacgagtc
    24661 tataaccgat gctatcaaga tgctctttgg ctactccgat gagtcttgtt tcatacttcg
    24721 cacaactctc gaaggcgtct gcacctggct cgatcaggca tttacagaat atgcccatat
    24781 aacagagaaa cccaagcctt cttttataaa tgctggcgat ggcaagcacg agcatccgac
    24841 tcaagagttt ctggacgaat tctcttttct agaacagctc aattggaaag aagaacatat
    24901 acacgtggct atggtgggcg acctctatca tggaagaacc gtgcactcca aggcagatgg
    24961 tctcagaata ttcaaggaag tcgaagtaga cctcatagcg cccccattgt tgtccatgcc
    25021 ttcacattat gtagaaaaaa tgaggtcgaa tggttttcaa gtccgcactt tcgaatccat
    25081 agatgagtac ctcgcacagg acaaggtagc gcccatctgg tacttcactc gccttcaact
    25141 ggagcgcatg ggcgaagctg tactcgagcg agctccttat ttgcgccaat cggtcacttt
    25201 caagaaagac tatctcggtc gactgcccga aggctgccgc ttctatcacc ccttgccacg
    25261 cgatcgcaac ttcccgacaa taccattttt cctcgatgat ctacctctaa atggctggga
    25321 tagccaatct atcaacggct attggacacg cataatcgag attgccatgc tatcgggccg
    25381 cattggagag gatttcgaag gtgaacatgc caagccacgc gaattcatcg acgactttgt
    25441 acatgaagca ccgatccaag agacggccaa gcctgaatac aaggtcggaa taaagcctgt
    25501 cgaggaagga atcgtcatcg atcatattgc aaggggagct cctataggcg aaatctggga
    25561 tactatcgat gctgtgcgca aaattctcaa gctcgatgtt cgttccagcc atggtgtcta
    25621 tcattccaac cgcgaaggaa tctataaggg catcatttct cttccggata taataagttt
    25681 tggcgaaaaa gacttgaaaa aacttgcggc gatcgctcca ggctgcacgc tcaatctcat
    25741 acgtcatgct catgtagcca agaagtaccg cctatccatg ccgccccgta tctataactt
    25801 cgacgaaatc tcatgcaaga acgaaaactg catctcatat ccggcacacc acgagggtgt
    25861 ccccaccgaa ttcatccgaa agggcgaaac gacatttgtc tgtaagtact gcgaacgcga
    25921 gcacaaattc agggatatct gggatatctg attgactttg ataactatcg atatattctc
    25981 tggacaacgg gcttggtatt aggaccaaat caggttttct atattaaatt ttctatgtca
    26041 gatgttatat gtcgaatcct atatttctta tgttcgatct caaatattcg aggaaccaga
    26101 gtttgagcgc caaaactcat acatgagaat tgccgcagat acagagacat tcaacgattc
    26161 gacatgccct tgcatgggaa tcgagataat atcgtcagct acctttctaa gtatttccga
    26221 gactccacgt ccttcattgc caagaatgat cacggctttt tgtgccatgc cagcgctcac
    26281 aagcggctta ccgcccatat ctgcggcata cacccaatag cctgctttct tgagagtatc
    26341 gatagccgat ctgagattgc tcacgcgcaa aatgggaacc catgcgatgg cacccgccga
    26401 tgccctggcc acggcattgg agatcggaga agtacgccgt tccggcatga taacggcatc
    26461 tacattgaag gcatcggcgg atctcaggat agcccccagg ttttgagggt cttcgatatg
    26521 gtctagcacc aaaacaaggc tcggctccgg ctcagagact cgatcacaca gagcttttag
    26581 ggaaggcttt cccttaagaa cttcatcgag cgcaagaatg gctccacgct gatctttcga
    26641 catctcctca agcctcgatt cagacacata ttggatcgaa atgttacgcg atagagcttc
    26701 atcgagaatc tctcgtatcc ggggacctcg cctttgagca ttttccgtta tatagaggct
    26761 gcaccgcgaa ttctcggctt tgagagtctc tagaattgca tgaaatgatg tcagatactt
    26821 cacgcaccat cctcacaaaa gattgcatat cgggttctat tcattatgta tcgatcacgg
    26881 aataacagta aattcctgga ctagctgaat agcgctctct acctgtttaa cgcctggcac
    26941 gagattcgct gtagcaacag cgctgtctac agtagcacgg ctattcgata ctccatggag
    27001 caccacaaca ccgcggtcca ccgctgcctc aaggaaatga atagggattt ttcgattgta
    27061 ggtgatctct gtcactattt ttgctccaag caatagatct ttaagctttg cctgagcttc
    27121 tttttctcta tcttcggtta taaaggacat tctaaaggca tcgatcgccg aagcggcatg
    27181 gattggatcg aagcgatcgg ttgtcaaagt catgtcatat tccgaaggat tgtaccagtc
    27241 cacggaaaaa aagtacttat gaaagccagc tcgatcatga tcgctgctct cgattatctg
    27301 aagggcatgg cgcgaatcta tattctgtgc tttgcgcact ctctcgattc taactgtttt
    27361 ggaagcgcca attcggatgc ttatcagatt cttgataccg cgtagaatta catgagcccc
    27421 tcgcccaata aagatacaat tattttctag cgctgtttca tagacggttt gggtgagaaa
    27481 atgcaggtat tcatccctct gctgagaaag cgaagcccag aaaccaggat ttttctcgtc
    27541 gtatcgagcc tgcttttcca catctattcc catcgccgaa agagccgctt cgatatgctc
    27601 cctatcgacg atcttatatc cattgagctt tgaaagctct tgagccgtct cttcgccata
    27661 agaagccagc tcgcgagaaa ttgctatcac tgccatatta cacctcccta taaaaaagca
    27721 taacccaagt taggcctcgg gtcaaacgaa tttcatagac ctcatcattt tatgcttggt
    27781 atagcgttgc caatattgaa tcctccagat ccccttctat ataagcccct tttataaaag
    27841 gaatcctttg gctagaatgc caagtgatgg agagatttgc gcttcttgcc gcatttggaa
    27901 ccgcggtttg ttgggctttt tcggcgatat tcttcgagac cgcctccaag aaagttggcg
    27961 cactggctgt caatttttgg aaagtggttt tcgccttttt gtttctcgga atatccggca
    28021 tggtcgaacg ggcggtgcct ttcccttatg acgcgccaat taggacatgg attctcttgg
    28081 gcttgtcggg gctcgttggc ttcgtaattt ccgacttttt tctttttaat gcatatatat
    28141 tgataggttc aagaattact gtcattttcc aatcgcttac gcctattttt actgctttct
    28201 ttgcctttat ctttataggc gaacggatgc aagcatatcg actcattgga atggcggtga
    28261 ccgttgctgg cattctaatt gttgtcacca ctcgttctcg tcaaaataaa aaagacaata
    28321 aagagaagct ctctgcaaaa ggaattttat atggctttct ttcctccatt tttcaggctg
    28381 gaggcatggt gctcagcaaa gctggtctgg gcgactatag cccaatctcc ggcacgcaaa
    28441 ttcgtgcatt cgtagcgatt tttggctttg caataaatgc gctgctcatc ggacaaggaa
    28501 gatttgtctt aatcgagatg cccaaaataa aagaggcctt tcgctcaact ttcaagggct
    28561 ccattttcgg cccatttttg ggtgtagctc tctcgctctt ctccatgcaa catacgcagg
    28621 tgggagcaac aagtacgttg atggctctta ctcccgtagt tatcattgtg ccatcggtgt
    28681 tgatactcaa gcaaaaagtc cattatctag aagtagtcgg cgcggcggtt gcggtaggtg
    28741 gagctgctct ctttttccta ttgtaagatt ttggacgact tctctctttc tttaagcgcc
    28801 acatagttcc aaatttaaaa aatagaaaaa ctgcaaatac gaaaaatcat agaaagatag
    28861 ggacccaatt taccgagaga tgatttcgat tattgcattt ctcatttcaa tatttggtag
    28921 tatacttgaa atgtgatttg atctacgcgc ctgcattccc atagaggagt agaatttatg
    28981 gatcgaaaat aattcatgca tcgaaaatta tgggaaacaa atgcgcaaag tcaaaaaagg
    29041 agcaatatga aagcgcttgt caaaaggaac gccgaaccag gactctggat ggaagaagtc
    29101 cctatacccc atattggaga taacgatctg ttgataaaag tcaagaaaac ggcaatatgt
    29161 ggcaccgatg tgcatatatg gaaatggaac acctgggcgc agcatacaat ccatatcggt
    29221 caaattgtag gtcatgaatt tgttggcgag attgtcgata agggtcgatc ggtacaagac
    29281 tatagaatcg gagagcgcgt ttcggcagaa ggccatatat ggtgcggtac ttgccgagca
    29341 tgtcgcgccg gcaaacatca tctctgcccc aatactattg gaattggcgt aaatcgagat
    29401 ggttgtttcg cggagtatgt ggccgtccct gcacaaaacg cttggcgtgt gcatgaagcc
    29461 atctccgatg atatcgcagc tatttttgat ccctttggga atgctaccca tacggcgcta
    29521 tccttcgata tgacaggtga agatgtcctg atcaccggag cagggcctat cggttgcatg
    29581 gctgcagcta tagcgcgcca tgtgggagct aggaatgtag tggtgacgga tgtaaatcca
    29641 tggcggctcg agcttgcaaa aaagctggga gcgacatgtg tggtaaatgt cgataaagaa
    29701 gacctaaaag aagtacagaa aagacttgga atggttggtg gcttcgatgt cgggatggag
    29761 atgtcgggca accccaaggc tctcgagcag atgatcgaaa acatgtacaa tggcggacgc
    29821 atggcgctgc ttggcctttt gccggatgga gctggtgtcg actggtcgag gatcatcttc
    29881 aaagggcttt tcataaaagg agtatatggc cgcgaaattt tcgagacttg gtacaaaatg
    29941 cagaccatgc tgttgtctgg gctggatata agccctgtaa taacccatag attctccttc
    30001 gatgacttcg agaaaggctt cgaggccatg ctctccgggg agtctggaaa agttatcctg
    30061 aatctggaat gatagaatac ttggttataa aattattggc ccatgactga aaataatctt
    30121 ctctataatt ttcgcataag cgaagatgcg gcatcggcct tggaaaggct tccgcactgt
    30181 atcgatgaag caaatcctat ttccatccag catcgcgcag agcttcgcta cagcattcag
    30241 agtctttggc gactattgac aatggagaga gcgcagcgta caagcgatta tctcaataat
    30301 cccgcctttt tttcggccta tctgcgctat ttcatgcctt ggaacctcgt gcggcttatc
    30361 gcccttttga cggatctttc ggtcgagctt aagccagatg ccaccataat cgatatgggt
    30421 tcggggccgc tgaccttgcc tttatcgctc tattgcgcca agcctgaatt gagaaagctt
    30481 cctcttacaa tcatctgtgc ggatcgagct cctcgaataa tggaggcggg aaaactcatt
    30541 ctggagattc tcgctgtaga gcataccgga gaattgccac catggaagat cgaattgaga
    30601 cacattaggt tcggagaacc gattaaggaa agggccgatc tcttctgcgc cataaatgtc
    30661 ttcaacgaat tcttctggaa aggcaaagga acgcttttcg aaaatgccag cgatctatat
    30721 cgatccatga tacggtattg cagccatgat gcttcaatgc tgatcgtgga acctggcgaa
    30781 cccagatgtg ggggatttct aagcgctctg cgcgccgctg ccattcagga taattgtgag
    30841 attttagctc cttgcccgca taactcggct tgtcccatgc ctggaatttt caaaagcggg
    30901 caagaacata ttatcgaaag aagtcctaga aacctagata taaaaaagga gcttggctcg
    30961 ggttcaattc taagatcggg ttttattcga caatcggatt ctcttcgaag atcggattcc
    31021 aatcaaagca aagataatga tttcttggcg cctgtccgca tgcctgctcc acgagtaaaa
    31081 tatccttggt gccatttctc catgcccacg agttatgctc ccaaatggct ctataggtta
    31141 tcgcaagaag ctggtctagc caaagaaaaa ctgtctttta gctatctcta cataaaacga
    31201 tatccatccc caatgtcatc ctcgaatcag aataaagaga aaaagagtcg gagtcctggc
    31261 aagccgccat tgctgtccga ggcaaataag ggacgcgatc agattgcttg tcgaattgtc
    31321 tccgaaccaa ttgcgctttc tcgttcaaga gtcggtagat atgcttgttc tcccctaggc
    31381 tatactctta tttcgaccgc acaatctaat tcgttgccag aatctggtgt tctcttgcgt
    31441 atacaaacga ggagcagcgc agcctccgat tcgacaaaga agatcgaaaa agaaacggaa
    31501 tatgaccaaa agacaggcgc tatcctcata tcctattgac acaaaactag ggcgccgata
    31561 tagtaccaag ccgtcagtca tgacaagggg gcgtagcgaa gctggttatc gcgccggcct
    31621 gtcaagccgg agatcgcggg ttcaaggccc gtcgctcccg aaagccatag agtagcgatg
    31681 tgtatgctgt aagcggaaaa gagctcttct ctatggttcc gcgtcatgcg gaataaaacc
    31741 cgtccgccgc gcgacgggct tttttttaag cattcgggct gtagcgcagg ggctagcgcg
    31801 cttggttcgg gaccaagatg tcggaggttc aaatcctctc agcccgaaaa ttttcgcgta
    31861 tatatagcat atctttgata gccaattatg tttggtgaat aaaaaagcag ccagatgctc
    31921 tatggcatgg ctgcttttaa gcctttaaga ttctatggtt caaactgtga ctaaaaaaca
    31981 aagaaagtca cggtcatagt atctgtgtag actccagatt catagatttg acctgctaaa
    32041 gcattgactt taattccaac agcgaatctt gcaccattct ttgaggtttt cccaatgaat
    32101 tgtatggtct tcgaacttgt gagttgcaca tatccgttca taatgtctga agacatgatt
    32161 atgccaccat aactatctgc aagctccgtt acctttacat aataggctat cttatctgtc
    32221 ccttgtttga cataacccag gtttgctgaa gaaaattgaa tatgaaaaat ccaagtgctg
    32281 cctgatttta ccgtcatgac gccttgctga tcattttgat aaggtgttgc cccatcattt
    32341 accagaggta tattccattg ataggaatca aataagatat ttgcgtctgc aggcagcacc
    32401 gttgcattta tagttacatt attagcccat actgctccgg atataagcaa tagcagacca
    32461 gtgatcgccc accatttttt cattttgcca gctctctttg ctcatttttc atccctgagc
    32521 tctgagaaaa actttgcaaa ttgaaagcat tcgtgctctc cgataatcag ttagcactta
    32581 tgccaatggt gagagttgcg gagtaatctc cattttcgta ataatcggta tattcattaa
    32641 ttttgaagct tatggggaaa atcttacctg caacgggagt ccttgcagtg aacacgatgg
    32701 tcttacctgt atctaactga acatagtcac ttagattatt cgttgaaact ccagagcccc
    32761 atcccgtagt atctactttt attagatagg gaatgtaata agactcagtt ccagaagtca
    32821 tcttcaattg acccgaatca gaagagctga aagttacagt atatgcatgc ttactggatt
    32881 tcactgtaag gttatctact ggtgaaacag tcacagctgt gcctgtatta tctagggtga
    32941 cactcca
//
LOCUS       DAOSOB010000014        32656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1391_length_32656_cov_63.695377, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000014 DAOSOB010000000
VERSION     DAOSOB010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 32656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32656
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1391_length_32656_cov_63.695377"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1064
                     /locus_tag="PLS88_02700"
     CDS             186..1064
                     /locus_tag="PLS88_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU06290.1"
                     /translation="MVQFLQQLINGLSIGSVYALMAVGYSLVYSIMNFSNFAHGGVIM
                     LGAYFGFFCLTLFKLPFALAFLLASLGTLVVAIIIERIAYKPLRERKAPFLYFIISAM
                     GTSIMLENLVIATIGPTFRTYPEVFPKVPIEIGSLAIGRLDLIIFVISAVSLTLLMIF
                     IDKTKLGKAIQAASYNIKACALMGINTDIVILTVFGLGGFLAGIAGVFFGMKYTVYPQ
                     IGNITTKSFIAAVFGGLGSLPGAVLGSVILGVIETFVAGYISSQFRDLIAFVILIGVL
                     VLRPRGIMGKVTEEKA"
     gene            1074..2006
                     /locus_tag="PLS88_02705"
     CDS             1074..2006
                     /locus_tag="PLS88_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU06291.1"
                     /translation="MDWTYIQGILMLAGINMITVLGLSLLTGFTGLFSFGHAGFMAIG
                     AYIGTWISASPASTPAGLGWPFIFSIFGGGIAAGLVSYLIGRITLKLKGDYFCIATLG
                     FGEAIRLIFNNVNRFGGARGWPGVPGKSTFLAILIADVIAVLVLANIVNSKHGRNMIA
                     VREEELAAQTAGIDVFRYKMISLVISAIYAGVAGVFLAHYMTFIQPKMFSLTKSTELT
                     IIVIFGGLGSISGSVLGALVLTALPEILRAFEIWRLVFYGAAVILIMVSRPKGLMGGM
                     ELTPSGIRKAMAERNALREAKAKLLAEAQEGEEQ"
     gene            2003..2791
                     /locus_tag="PLS88_02710"
     CDS             2003..2791
                     /locus_tag="PLS88_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06292.1"
                     /translation="MTASPILHIKDLTKRFGGLIAVDNVSFDVPEGGIIGLIGPNGAG
                     KTTIFNLITGIYKPTSGSIEFKNNSLLGLEPFRIAEKGITRTFQNIRLFKNLSVFDNV
                     LTACHRNANYNFAEAIFRLPRFRTQEKELMEKTEQLLDIMGLLCYKDLIANNLPYGLQ
                     RRLEIVRALALNPILLLLDEPAAGMNPDETEQLMGLIARIRDDFKLSVLVIEHHMDLV
                     MGVCENIVVLNFGEKIAEGNADEISKNPLVIEAYLGTPEEAANA"
     gene            2784..3506
                     /locus_tag="PLS88_02715"
     CDS             2784..3506
                     /locus_tag="PLS88_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06293.1"
                     /translation="MLEIKNLHVAYGGIRALKGVSLSVQEGEIVTIIGANGAGKSTLL
                     NTISGFLRPAKGTIQYRGRPLPRRPDLIVKAGICQVPEGRLIFANLSVLDNLLLGAYR
                     RSDKKAVNADLKKVFEIFPRLKERQSQRAGTLSGGEQQMLSMGRALMTNGELILMDEP
                     SLGLAPLLVQTVFEIIEQFRGLGKTILLVEQNAFKALQVADRAYVLEQGRIVKQGLSR
                     DIAQDPAVKEAYLGSRTRGGGG"
     gene            complement(3509..4123)
                     /locus_tag="PLS88_02720"
     CDS             complement(3509..4123)
                     /locus_tag="PLS88_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOU06294.1"
                     /translation="MPDFRTSNPIAIILAAGCSSRMGATKPLVEIDGIPMLLRAANCF
                     QEAGISEIIVVTGFDADKVGKLAGSHGMRPIFNNDYRKGMFTSICAGLRASSSICDAA
                     FILPVDIPFVLPSTVVELKNTIMDRPIVIPRYGKTSGHPPIIHRALFSLVENWDGENG
                     LRGFFDYRKADIEYLEVDDANILLDIDTKADLKELLLERARLRS"
     gene            complement(4163..5362)
                     /locus_tag="PLS88_02725"
     CDS             complement(4163..5362)
                     /locus_tag="PLS88_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOU06295.1"
                     /translation="MNELRSWIIERLNSNDTVVLVAVTHASGSTARGTDALMAVDMNG
                     RMEGTVGGGYVENQAIIAIRKLLEIGGDHQDLFFDLSPEAQQNQMTCGGKVSLHLERV
                     DPQSEACNALKACFERLESGSACSFLVARAPEEKHCFAIDEKGNVLYPHLQTEIQELI
                     AKNIVEDIKKLQPDLNGSSGLSSSSSLCSASCRSDTTGLKSAQAFCGASCFELELPEA
                     SKINMARVFQLGFRPDPVAYIFGAGHVGKATSVAASLVGFQVVITDDRPELLTRERFP
                     KASMLRIIDSFADPLAASRDAPAIEIGPQDCAMILTRKPDIDKEMLSCLLRTKAGYIG
                     LIGSKTKRDGIFAALREEGFSDIDLSRVHSPIGLGIGAETPEEIAISIMAEVIAVRSG
                     VLTQLKC"
     gene            complement(5367..7760)
                     /locus_tag="PLS88_02730"
     CDS             complement(5367..7760)
                     /locus_tag="PLS88_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU06296.1"
                     /translation="MIDNFDSIPPADSKTLKATQKFNVIAHDIAKIDGEGLVLGRPAF
                     ADDLAPANALYVKLVRSPHAFARIISIDPSEALALPGVACVLTWKDCPRVPITRAGQG
                     NPEPSPHDRFILDEYVRYVGDEVAVVAAEDEATAEKAAMLVKVQYEVLQPVLDFEKAL
                     DNPIVIHPEPEIHEMFPIGFEPKRNIAAAYNMEIGNVEQELAKSPVQVETTVHTQAQQ
                     HVALEPHTAFSYIDIHGRLVIVTSTQNPWHTRRLLALALQMPLRQIRIIKPRIGGGFG
                     GKQHIHIEPYVAMVTLKTGKPARLALTRREVFEATFTRHEMRVKVRLGANSDGKLNAI
                     DMQVLSNTGAYGEHALTVFMVAGSKTLPLYNKVNAVRFGGHVVYTNKVSAGAFRGYGA
                     IQGLTGLESAMDELAHKIGIDPVELRRKNMIHEGETSDVFRIMGEGTEGVAMTIESCK
                     LEECIARGKELIGWNPDRLVWEVAPGKVRAMGMAIAMQGSGLPLIDMGSARIEFQDGG
                     FFKLHVGATDLGTGSDTILAQIAAEELGVDVESIVVYSSDTDNTPFDVGAYASSTTYV
                     SGSAVVKAARSLKKKLIEAVAQKFGVDPAFVIFDNKTFKTLNGSKSLSLYDFSFDTLY
                     HDGERMKTIEATESFTGAKSPPPYLAGFVEIELDKETGKVEVVHYVSVADVGTPINPN
                     LATIQIEGAALQGIGLALYEDVRYSERGHMLSHNLMTYAIPSREDVGRITVELVDSYE
                     PSGPFGAKSAGEIGIDTPPAAISNAIRNAIGIRLTDFPFTPERVLMAIDKAKTEGAN"
     gene            complement(7753..8208)
                     /locus_tag="PLS88_02735"
     CDS             complement(7753..8208)
                     /locus_tag="PLS88_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOU06297.1"
                     /translation="MTITIYINGKREILECQPGDMLTEVLRKAGYVEVKKGCDTASCG
                     ACTVLIEDKPVLSCSYLAVRADGKRITTIQGVAEEAKKFAQFLTAEGADQCGFCAPGF
                     ALTVLSMKKELRNPSEAEIRHYLSGNLCRCSGYEGQIRAIKKYLEAVHD"
     gene            complement(8205..8993)
                     /locus_tag="PLS88_02740"
     CDS             complement(8205..8993)
                     /locus_tag="PLS88_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOU06298.1"
                     /translation="MEIREYARPKSMDEAMHLIHEEHGTAIGGSAWLRTNTKTVMLGV
                     DLSDLGLEYIREFQDHIEIGAMTTYRELETSLLLASRFGKIFSTAVGHIVGIQLRSII
                     TVGGTVAGRYGFSDLNTTLCALGARVVFYPNIEEDLASFIEKGYAGPFLIEKIILPSS
                     ARAAYQQMRISENDFPIINTAVAWMGTEWRVAAGSRPAAARLCRNAMKILADSANPSN
                     EQIEAAAQESSKELQFGSDIRASATYRQKILPVLVRRALLEVRG"
     gene            9154..12570
                     /gene="dnaE"
                     /locus_tag="PLS88_02745"
     CDS             9154..12570
                     /gene="dnaE"
                     /locus_tag="PLS88_02745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOU06299.1"
                     /translation="MAEFVHLHNHTDYSLLDGAASIPKLIRKAKSLGMPGIAITDHGN
                     MFGAMSFEKECRDFGLNPIIGCEFYVAGGSRFEKTGMENGNKYSHLILIAENSIGYRN
                     LLKLTSASYTEGFYYKPRIDDELIVANAKGLIASTACLAGEIPSLILSGKLDQATKKI
                     SFYKELFGEDHFYLELQDHGLDEQKVVNRELVRLSKKLDLPLLATNDIHYVEEGDAIA
                     HDILLCIGTNRKRDDSSRMRFPNDQFYLKSTEEMASIFAEVPEAIVNTLKINEMAALK
                     IEFPGPLLPDYQVPEEFSSPEEYLRHIARRGFQSRYPNPDAEVIQRLEYELDIIIKMG
                     FTGYFLIVWDFINWAKEHGIPVGPGRGSGAGSIVAYSMRITDIDPLKYDLLFERFLNP
                     ERVSMPDFDVDFDFERRGEVIDYVTQKYGSDRVAQIITFGTLKAKAVIKDVARALDIP
                     FDEANQIAKLVPEDPKMTLHKAIKMEPKLAALEHNPSYSELFSIAAKLENLHRHSSIH
                     AAGIVIGKTALIDYVPLYKDPKTGLIATQYTMEYLEGCGLVKMDFLGLKTLTLIRNTM
                     ELLRKRGIYLKEEDIPEDDSKTFKMLSEGKSTSIFQFESQGMQSILKRAKPSSIEDLI
                     ALNALYRPGPMEFIDQYIDSKNGKIAISYPHPSLEKYLKGTYGVIVYQEQVMQVAREV
                     AGYSLGRADLFRRAMGKKKPEILQKEEEPFIEGALARGYSREEAKRIFDILTPFAGYG
                     FNKSHAAAYSVIAYRTAYLKANYSAEFMAANLTNEISNTDKLTEYISEARQMGLSVLP
                     PDINRSDAKFTVDNGQIIYGFLGIKGVGEGIAMDIQKERESHGKFKDFLDFLTRMNDA
                     GINRKTMESLIMAGCFDSFGKGRHELMENFERAMNYVHEKLSFEASRSGMLFQEEDLG
                     IYPDFDFLPFEEFPNQEMLRAEKELLGFFFSAHPMDEYKMIWERSSTLDLGNLEKESV
                     NHEYVVVALLKEFRSYTTQNGRRMAFGKLEDYNGSIEIVVFPDLFEKNEASFVKDRVL
                     CLQGVLDNSRRGFSFKVQAVLDPTSLKSNSWRELHIRLTDAAIDETALYPLRDALYSI
                     QGKCRVIFHVPLKGGGEALIEAGASTRCSASDEDLAFIERQAAVTEVWRE"
     gene            12567..13403
                     /locus_tag="PLS88_02750"
     CDS             12567..13403
                     /locus_tag="PLS88_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU06300.1"
                     /translation="MNIKILGNGGFYNEGLAYNALAIDGHVLVETPPDILQSLHSQGM
                     KPSQIDTVYISHNHGDHCFGFPFFFFNWLYAEGSDSHNEKQAELVIMGPKGLKEHLGS
                     LMRLAILPEHQYLSEFDQRVRFIEVDEKTVLPVKGGLWFGFLRTKHSVPTFSLIVGKA
                     GHENELPSCCDYLEKALFIYSSDTSMFDGIYKLLNSSAKLILCDTNGEEENGVHMSPR
                     ELMAAKSHIRSRSEECAVLSEQRQSVGNDIDRVLGIHLSRKMDRVGQLRFAQPGEEYC
                     IK"
     gene            complement(13431..14117)
                     /locus_tag="PLS88_02755"
     CDS             complement(13431..14117)
                     /locus_tag="PLS88_02755"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU06301.1"
                     /translation="MEGRSRLRAVAFDVDGTLYPESSIALRCVDLFFGQPRIFMAFST
                     VRKEIRFLQAQPNYIPRNMEELHSLQVSLLAKRLKMDESHIEQSIEKIFYHLVPERFS
                     SIHPYRGTRQAIEAIRQNGLIIAALSDLPPQEKLQFLGLHDLFDYKLCTEEWGVLKPH
                     QRAFDGMVKTIRVQPFETLYVGNNIEYDILGAKKAGLRAALRGRQNSAADLSFSNWEE
                     LTQWVLAHSY"
     gene            complement(14219..15988)
                     /locus_tag="PLS88_02760"
     CDS             complement(14219..15988)
                     /locus_tag="PLS88_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOU06302.1"
                     /translation="MENLIDITIDGQTIRVDSNENIVEACARAGVKIPTLCFLKDISQ
                     NASCGVCVVEVEGAKSLLRSCVQKPTPGMKIRTSSPRVMRARKAAVELLLANHPDECL
                     SCLRADTCELHTMANLLGVRADRFPGYKKYPPADNSSEGIIRDDRKCILCGRCVSVCE
                     ETQGVSAIAFTGRGTRTRVATFMDRGLAQSACVQCGQCSLVCPTGAITEKDESQDVYG
                     ALGDSRLSVVVQTAPAIRASLGEALGLPAGSLVTGQMVAALRRLGFSKVFDTQFAADL
                     TIIEEGNELLERLSHGGTLPMITSCSPGWINFIEGFYPDLLDHVSTCKSPQQMFGAVA
                     KTYYAKNANIAPKNMRVVSIMPCTAKKYEARRKEMISAWDWWKDKDPESTGLRPFFDV
                     DWALTTRELARMIRLAGIDITALPEEDFDDPLGKSTGAATIFGTTGGVMEAALRTVYE
                     IVEKKPLESLDFIQVRGFDSIKSAEVLLGGSPVRVAVAHGLSNARILLDEIRAGKSRY
                     HFIEIMSCPGGCVGGGGQPVLADIEKKKARSLSLYEEDRRLPIRKSHENPAITTLYEE
                     FLGKPLSHLSHELLHTSYKARVF"
     gene            complement(16002..19196)
                     /locus_tag="PLS88_02765"
     CDS             complement(16002..19196)
                     /locus_tag="PLS88_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU06303.1"
                     /translation="MINNTDLGFLGQEGSSHFHGNTPWISVGMGTCGIGSGADKVWEI
                     LSQKTENTNIHLRRVGCFGFCAAEPVVMAWRPGKPILFFTEVNTQKASRILAGLSSDA
                     TYDKIAKIAEAKIEEWDFRTSKVDFGRGYDNLPTWKELNFFRDQEKIILRDAGMIDPE
                     SIEEYIAVGGYGSLVRALTSMPPEAIIEEVKKSGLRGRGGAGFPTWKKWSLMRKSLID
                     NPGEAYLICNADEGDPGAYMNRNEIESDPHMLLEGMLLGAYAMNVNKGIVYVRAEYPL
                     AVYRLNLAIESARQHGLLGRNILGSKFSFDIDMVTGAGAFVCGEETALIASIEGNAGR
                     PRPRPPFPVEKGLYGRPTSINNVETWCNIPVIIARGGEWFAGFGTPMSTGTKVFSFVG
                     KVRNTGLVELPLGSTLESVIYGICGGMGPKRKIKAVQCGGPSGGCVPASLFKTPIDYE
                     HLTELGAIMGSGGMVVMDQDNCMVDVARYFTGFVMGESCGKCTPCREGTSQMLHILEK
                     VSDGEASEEDLDTLEEIATIVKDSSLCGLGQTSANPVITTLRYFRDEYIEHIKGKRCP
                     AGVCENLYIALCESSCPLHMNIPGYLQLLKENRIEDAFELTLRENPLPGTIGRICHFH
                     CRMRCRRDMLDEPVSQGEIHRYLADTMYKMGREKQVYTKLLKEKLPPSGKKIAIIGAG
                     PAGLTAAFYLVRLGHEVTIYESRQEAGGVLRWGIPSYRLPKDILKKEINLIEKLGTRF
                     VYNTRITEPEQWKKLADENDAIIIAAGASSELSMNIPGEDALGVYKACDFLNALAKKE
                     KVHTGGEVVVIGGGNSAIDAARSALRLGATVRIVYRRSKADMPANLDELKGALEEGVE
                     LICMASPLEIVTQEQQGKRVAKAVRIQRMKAGPIDPTGRPTPVPTDKIEDIPCSMVIM
                     AIGEKVEIPGIEALGIERLKNGRIKADPFSHITSNPKVYAIGDAMLGPATAAEAMGQA
                     KTVAEIIDQALSGKKRFDSLFRRFDYRMEIPANISKEKMTQPTMLPVNARKSNFMEIN
                     LGYTGEQARIEAYRCLRCDVREHRREPRGSLVGE"
     gene            complement(19193..19708)
                     /locus_tag="PLS88_02770"
     CDS             complement(19193..19708)
                     /locus_tag="PLS88_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOU06304.1"
                     /translation="MTKTISNSEPLNVESIIDSWKGRNGSLLGALEQAQQNDPKKYLS
                     ETTLREISKRLDIPLSRVYSVATFYSFFSLKPQGEHLLIVCRGTACHTRGSLVLLNEA
                     LSRLKIKEFNEKEENSVTSSDNLITIRTVACFGQCALAPVIMVDGLVLSRMTVSKLVS
                     LLDKLKKGGER"
     gene            19894..20829
                     /locus_tag="PLS88_02775"
     CDS             19894..20829
                     /locus_tag="PLS88_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06305.1"
                     /translation="MALNDRENEEISITRPKWPIKFTNQSVDCVGRKLKRIFDQNASS
                     DYFTSDEIRDYSYYERLLLQELEGMDRKISKGKLKPSHIVEYPRVIHADEGKIRACIY
                     IGSFDPFQLTHLTVAMRFLASDLSNSDFVIIVPEGSPDTAKPLKTDYAFRYSIAKMQI
                     EGIFDSFIKVLDLGTQADTIEIIRRFIAMHSGFTLELTHLIGSDSLPIAASLISQDMR
                     IWRKEAQESKVDYHHSLYVIQRGDVPEIRPYLEAFEEEGIQVVIDPFSVEAPSSSDFR
                     TKQAYTIVLPTNSIREKMEIIFRYHMHKTWSSDRN"
     gene            20934..21575
                     /locus_tag="PLS88_02780"
     CDS             20934..21575
                     /locus_tag="PLS88_02780"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOU06306.1"
                     /translation="MKLIFLGPPGAGKGTIARMASERLGIPQISTGDIFRTAVKEKTE
                     LGLKVQSILAAGALVPDELTIAIVKERLSWPDVRQSYILDGFPRTIAQAEALASFSKP
                     DSAVDFEVDDDLILFRLTGRRVCKSCGAIYHIVTKPPATEGICDICGGELYIRNDDRE
                     ETVKIRLKAYHEESEPLIEYYSRQGILMTIDGSPDPETVYDTFISKIGRFLKK"
     gene            21572..23041
                     /locus_tag="PLS88_02785"
     CDS             21572..23041
                     /locus_tag="PLS88_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU06307.1"
                     /translation="MSVLRALFHKKVEFFPSLFFLLMILTIVSVLPNGIAGAEEIVPG
                     VWKGVLLLREVGQKQSSAQLPSYSMDQQSTPFSFEFYLRILSKERGLLVDIPEQGLYS
                     YPIDEYTFDAQQISFIFDAIEGDEELRFLGNYSTSFLPPGSTHRGAIVGSVRGRSWNG
                     SFYVQKQDSSTDPGQISLDIPVEGGALPATLVFPLRELPFLDPNSPPDFPLILLVAGA
                     GRADRDGNNVDVPGKIDSLRQLSNMLRMRNVGTLRYDKRGTGEAYKLEKPGRMTSFSE
                     HVRDLTSVIIAAAALPRHGRLIVAGMNEGAWIAMSALEDEKVASLVDGFIVIDASGQS
                     PIETLKQSIASLDEELRAKALEAARNLVQRGILIDVPVALADFFAPSRKDWLATWLAF
                     DPVETIKQVQLPILLIYGENDLQISREEFSMLVEARPEAAVRIVPEMNYVLKEVKNED
                     ENYAAFTDPSFKVPSLLADLLASFAKAQPAPEGFVPWRL"
     gene            complement(23044..24045)
                     /locus_tag="PLS88_02790"
     CDS             complement(23044..24045)
                     /locus_tag="PLS88_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06308.1"
                     /translation="MGFLPNNRKEKRAKNTPIASIIILATICIYIVSCSSVPKEIAIA
                     GIFDHFEKTPQVILRSGDIFLRDIAASLDDSTIEALEAIASDKPNESEEMKEPSKPID
                     RAKIDKLLTRAQVAGIGISLTDKGQPALEAVFAGNFPDLLTSLSFSLDSNWKKINGGY
                     ALKSGSLYVRNPDRGQVHLATWMPPVSPLTNPGATALATKSGVLANAVDFAIYIDAKS
                     TIVSQLPIMEGVTLPFEGILMSATREATSPPKGSPSAMYRTIFKIQMKDEQGARTYKP
                     IVRFLWVMLSSKLGSYGIPITPETPIEQQGSVFVSQPVWISAQQLVDVALSISSFNN"
     gene            24284..24733
                     /gene="ndk"
                     /locus_tag="PLS88_02795"
     CDS             24284..24733
                     /gene="ndk"
                     /locus_tag="PLS88_02795"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOU06309.1"
                     /translation="MATERTFAMLKPGVLSRRVVGEIISKIERKGFKIIGLKMISVTR
                     DLAERQYAEHKGKPFYLDLLDYITSGPVLVMVLEADQAIKLFRLLCGLTSPDQALPGT
                     IRGDYALHTSTNIVHASDSKESAAREIGLFFKPEEIIDWEDANQKWI"
     gene            24801..26606
                     /locus_tag="PLS88_02800"
     CDS             24801..26606
                     /locus_tag="PLS88_02800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOU06310.1"
                     /translation="MSKTAEIPCWNLDDVFHGFESEEYKKAKNELKTLLENLGIELEN
                     AKPPSDIKSTANWLFELLGKLDRLETLADTLSAYVQARFSTDTRDALVLQELGKVEEL
                     LVPATKILVLFRNLLARYEKRIKKVISQDSRFVDYAFVLEEALMFQKHQMLPQLEDLA
                     ADLGRSGADAWSRLQDSILANASAIWNEATGERKTMVELRNLAYDADRSIREKAYRLE
                     LSIWKTYEIPVAAALNGVKGTVLTLNKRRGWESAIDASLAQARISRATLEALIGTMEE
                     SLPMWRRYLHAKARLLGLERLSFFDLFAPLQKQGAMIKSYSWPEAKDFIVRQFGSFDE
                     EMARFAEKAFENRWIDARPREGKVGGAYCTHFPAAKQPRVLCNFDGSYSSLITIAHEL
                     GHAWHYENIKDLPTLLSQYPMTLAETASIFSETLVSNAAMAAMEKDARLPLIELHLQD
                     ACQVIVDILSRYYFEHDVFEERAKGEITPDRLCDLMLQAQAKTYGEPLREDERHPYMW
                     AVKSHYYIPGLSFYNFPYAFGQLFGMGLYSIYKKEGQSFAAQYRELLRSTGSLPAAEV
                     ARRAGFDIEKPAFWQTAMKAFEHEVEFLEAQSCNR"
     gene            complement(26622..28208)
                     /gene="lysS"
                     /locus_tag="PLS88_02805"
     CDS             complement(26622..28208)
                     /gene="lysS"
                     /locus_tag="PLS88_02805"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOU06311.1"
                     /translation="MEQNKSIHWADQTAEKIMDTWGEKDLYTCASGITPSGTVHVGNF
                     REMISVELVVRALRDRGKNVRFIYSWDDYDVFRKVPLNMPNPELLEKYLRFPITMVPD
                     PWGRDESYARHHEVDVEKVLPEVGIYPEFIYQAQRYRSGVYASSMRRALEMREHLKTI
                     LDKYRDEDHKIQGEWWPVSVFCSNCNRDTTEIDEWDGEWGLSYHCETCGFKEKGDLRT
                     LKGAKLVWRVDWPMRWAYEKVDFEPAGKDHHSQGGSFDTSKHVVEEVYGRKPPVTFRY
                     DFIGIKGSPGKMSSSKGKVVDLQDLLRVYQPEIVRYLFAGTRPNTEFVISFDLDVIKI
                     YEDYDRTERIYWGVEKAKNKEVEALERRIYELSQVGKTPEEMPYQVPFRHLCNLLQIH
                     LGNIDAVLSLLPGLKPSQEARVRRRAQCAWYWITECAPEDFRFFLRKPGELASLPEAE
                     TNALRCLRDEVIERLDAYDSENEIAEAIYAVAQASNIEPKKLFIAAYQALIGKDQGPR
                     LAGFLRTLGKEKVLSILKPY"
     gene            complement(28299..29396)
                     /locus_tag="PLS88_02810"
     CDS             complement(28299..29396)
                     /locus_tag="PLS88_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M17"
                     /protein_id="HOU06312.1"
                     /translation="MYTLTEVAQVALTQCLDVKESERLLIVSNPETEQEEIAKAFAKQ
                     ASAMGAKVRFLLQPVKTQKDFAEDEVIGAIEESPNVVLSISTEKLGKDKARLASPLLA
                     PNGRSYDHIFDYLLHGVKAMRAVWTPGITKDIFLRTVPVDYEVMRRSAALLASFLDQA
                     STVLVRSPSGTDIVFSILGRKAMLDDGDFRYPGKGGNLPAGEVFISPALKSAEGTIVF
                     DGSISDIERDIVIEEPIVCTVRGGYVIGIEGGNEAKRLDTALIQGLSMASDLVSEQGM
                     SPEMALAYGTNARHLGEFGIGLNPAAHISGNMLEDEKIMGTIHFAIGSNYDEDAPALI
                     HLDGLVRFPTVVLLMPTGEEIAIMEQGVLAL"
     gene            complement(29396..30415)
                     /gene="tsaD"
                     /locus_tag="PLS88_02815"
     CDS             complement(29396..30415)
                     /gene="tsaD"
                     /locus_tag="PLS88_02815"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOU06313.1"
                     /translation="MKILGIETSCDECAAAIVEDGRHILSNVIMTQIPLHERYQGVVP
                     EIASRAHIEWITGVVKRALFEAGIEPSSIEGVAATAMPGLIGSLAVGLSFAKALAWSM
                     KLPFKGVNHMLAHLYAPQLIEPIDYPFLGLLVSGGHTILCRAEDFNRIEVLGRTIDDA
                     AGEAFDKVAKYYGFGYPGGAAIDNLAEKGDEKAFKFPMPSLHKGEHRYDVSYSGLKTA
                     VIYHLSSYLRPGAEVTPENIAASFRKTAIDILLSRLYRAVQDTGINTVVVGGGVAANA
                     YLRRKLEVKQDLYVYFPPISLCGDNGAMVAGIAWHYFQRGETDIWNLAPNSRFADFKW
                     HQEDG"
     gene            complement(30396..31460)
                     /locus_tag="PLS88_02820"
     CDS             complement(30396..31460)
                     /locus_tag="PLS88_02820"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOU06314.1"
                     /translation="MRKKRTHKGSYYAILLSSAAFLAIAALIIVNSINSKDTFSRTKD
                     VPYGTPEYVIELPPGYEDLAVIPPEERKDKTSISKRTASDLDIQAGAEEEIKKAGSEK
                     LEPYSSAGPPSALPGSVAQPTLIIVIDDVGYNNGELNAFLQLPFPITLAVLPQLPHSN
                     DSVAAIHQAGKELILHQPMEALGGNDPGPHAVYCSMDEATIEKTVAENIDSFPYPPAG
                     MNNHMGSAVTRDSNCMIPILKLTKERGIYYLNSLTAPGTVCAELSQKIGTQYMERDVF
                     LDNNSDRESILAAIEEGKQIARQKGAAVMIGHVWSSKLASTLMEIYPELVEQGYSLST
                     ISQYMKIQAKENLVNENFGD"
     gene            complement(31492..32313)
                     /locus_tag="PLS88_02825"
     CDS             complement(31492..32313)
                     /locus_tag="PLS88_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOU06315.1"
                     /translation="MTIADNDENLELYFESIRKIPLVTRQEEAVLAGKIAKGDKQALD
                     RLVEGNLRLVVRIAKSMWSPPHSLMDLIQEGNIGLVKAAERFDPSKNVKFSTYAVWWI
                     RQAISRSLVNTGRHIRLPHRKENALRRLSAIQMRASLELSRYPSEKELASETGWKETE
                     IAAILNMGDQPVSLDQTNNDELSILDVYEDWRYNPELEIEKSSRTWQSSWLLSTLPER
                     EQEVISKRFGLGCSREQSLKAVGSIVGCSAETVRHIEKRALESLRHTALQVGLTA"
BASE COUNT         8651 a         7349 c         7539 g         9117 t
ORIGIN      
        1 cacaacccgg aaggcaagga tgctgcaatc ctgaagatag tcaatggaga gtatatattc
       61 caggagaaat acgcagccca gtaaggaact ggacaaaaag gcaattggcg gccaactgcc
      121 gattgccttt ttctttttta taaagaaagt cggcaatcca taaaatgtag ttcaaaggaa
      181 gggcaatggt ccaattcctt caacaactta taaatggctt atccataggc tctgtctacg
      241 ctctcatggc ggttggatat tcgcttgttt attcgataat gaatttttcg aactttgcac
      301 atggtggcgt aataatgctg ggggcttatt ttggattctt ctgtcttacg ctattcaagc
      361 tgccatttgc gttggcattc ttgctcgctt cgttgggaac tcttgtagtt gctataatta
      421 tagaacggat agcctataag ccactgcgtg agcgcaaagc cccatttcta tactttatta
      481 tttcagcaat gggcacatct attatgcttg aaaaccttgt gattgcgacg ataggtccaa
      541 cttttcgtac atatcccgaa gtattcccaa aagttcctat agaaattggt tctctcgcca
      601 ttggaaggct agatctcata atcttcgtta tctctgctgt gagccttacg ctccttatga
      661 ttttcataga taagacaaag ctaggaaagg caatacaagc ggcctcatac aatatcaagg
      721 cctgcgccct aatgggaata aataccgata tagttattct tacagtattt gggcttggag
      781 ggttcctggc aggaatagcg ggtgtatttt ttgggatgaa atatacggtt tatccgcaga
      841 ttggcaatat tactaccaaa tcttttatcg cagctgtatt tggcggctta gggagcttgc
      901 ctggagcggt gctcggctcg gtaattctgg gtgtcattga aacctttgta gctggatata
      961 tctcatcaca gtttcgagat ctgatagcct ttgtgattct aataggcgtt cttgtgctga
     1021 gaccgagggg tataatgggc aaggttactg aggaaaaggc ttagggggcg agcatggatt
     1081 ggacatatat tcagggaatt ctgatgctcg caggaataaa catgataaca gttctgggct
     1141 taagtctttt gacaggtttt acagggctgt tttcgtttgg gcatgctgga ttcatggcaa
     1201 ttggagccta tattggaacc tggatcagcg cttctccagc atctacgcca gctggccttg
     1261 ggtggccttt tatcttttct atatttggtg ggggaatcgc cgctggcctt gtgagctatc
     1321 tgataggtag aataaccctt aagctcaaag gtgattattt ctgcatagca acgcttgggt
     1381 ttggcgaagc catccgcctc attttcaaca atgttaatcg ttttggcggg gctaggggtt
     1441 ggcctggggt tcccggaaag agcacttttc ttgccattct tatagcggat gtcattgcag
     1501 tgcttgtcct cgcaaacatt gtaaattcta agcatggtag gaatatgatt gccgtgcgag
     1561 aagaggaact cgctgcacag accgctggca tagatgtatt tcgctacaag atgatctctc
     1621 ttgtaatcag cgcaatatat gcaggggttg caggggtgtt tttagctcat tatatgacct
     1681 ttattcagcc caaaatgttc tctctcacca agtcgaccga gctcacaatc atagtcatat
     1741 tcggagggct cggctctatt tcaggctcgg tcttgggggc tctagtgctc accgctcttc
     1801 ctgagatttt aagggctttc gagatatgga ggctcgtatt ctatggagcg gcggtcattt
     1861 taattatggt gagcaggcca aaaggcttga tgggtggtat ggagcttaca ccatcgggga
     1921 ttcgcaaggc aatggcagaa agaaacgcct tgcgcgaggc aaaagcaaag cttcttgcgg
     1981 aggcgcaaga aggagaagaa caatgacagc ctcgcctatt cttcatatca aagatctaac
     2041 aaagagattt ggtggattga ttgcagtaga caatgtgagt ttcgatgttc ccgaaggagg
     2101 cattataggg ctaataggac ctaatggcgc agggaagaca accattttca acttgatcac
     2161 gggaatatat aagccaacct ctggttcgat cgagtttaag aataattcct tattaggcct
     2221 tgagccattc cgaattgctg agaaaggtat aacacggaca tttcagaata taaggctttt
     2281 caagaactta agtgtattcg acaatgtact gacagcctgc catagaaatg ctaactacaa
     2341 ttttgcggaa gcaattttcc gccttcctcg tttccgcact caggaaaaag agctcatgga
     2401 aaaaacggag cagctcttag atatcatggg gctcctatgc tacaaagatc tcattgcaaa
     2461 caatttgcct tatggcctac agcgaaggct ggaaattgta agggcgcttg cgcttaatcc
     2521 aatacttctg ttattagatg agccagctgc aggtatgaac cccgatgaaa ccgagcagct
     2581 tatgggcctt attgccagaa tacgagatga ttttaagctc tcggtccttg tgatagagca
     2641 tcacatggac ctagtaatgg gtgtttgtga aaatatagtc gttctgaatt tcggcgagaa
     2701 aattgccgaa ggtaatgcgg acgaaatatc gaagaatcct ctggtcatcg aagcctatct
     2761 tggaacccca gaggaggctg ccaatgcttg agataaagaa tttgcatgtt gcctatggcg
     2821 gcattcgagc gctaaaaggt gtttcgctct ctgtgcaaga aggtgagatc gtcacaatta
     2881 tcggagctaa tggcgcgggg aaatcgacct tgctgaatac tatttctgga tttttgaggc
     2941 cagcaaaggg gacaatccag tataggggta ggcctctccc tcggcgccca gacttgattg
     3001 tcaaggctgg tatctgtcaa gtgcctgaag ggcgcctgat attcgctaac ttgagtgtac
     3061 tcgataatct tctacttgga gcatatcgac ggagcgacaa gaaggctgtc aatgccgatc
     3121 tcaaaaaagt ctttgagata ttccctaggt taaaagagcg tcaatcccaa agagcaggaa
     3181 cgctctctgg tggggaacag cagatgcttt ccatggggcg tgctctaatg actaatggag
     3241 agctcattct tatggatgaa ccctcgttgg gcctagcccc tcttcttgtc cagactgtct
     3301 tcgaaataat cgagcaattc cgagggcttg gcaagactat acttctggtg gagcagaatg
     3361 cattcaaggc acttcaagtt gcggatcgag cctatgtgct cgaacaggga agaattgtaa
     3421 agcaagggct atctcgcgac attgcccagg atcctgctgt aaaagaggct taccttggtt
     3481 cgcgtactcg aggcggggga ggctgagctc aagagcgcaa tctagctcgt tccaggagta
     3541 gttctttaag gtcggccttg gtatctatgt cgagaagtat attagcgtcg tcgacttcca
     3601 aatattcgat atcggccttg cgatagtcaa aaaatcccct tagtccattc tctccatccc
     3661 agttttccac tagcgagaac aatgccctat gaatgattgg cggatggcct gaggttttgc
     3721 catatcgagg aatgactatg ggtctatcca taattgtgtt cttgagctcg accacagtgc
     3781 taggcaaaac gaagggaata tcgaccggca gaataaaagc agcatcgcaa atagaagagg
     3841 aagctcttaa acctgcgcag atcgaagtga acattccttt tcgataatca ttattgaaaa
     3901 tggggcgcat tccatgtgaa ccagctagtt tgcctacttt atcggcatcg aagcccgtaa
     3961 ctacgattat ttcgcttatc cccgcctctt gaaagcagtt tgctgcacgc aaaagcatgg
     4021 gaattccatc tatttccaca agaggtttgg tggcccccat gcgggaggag cagcccgctg
     4081 ctagaatgat agcaataggg ttacttgtcc taaagtctgg catagaatta aaatcctctt
     4141 gcacggagcc aatttatgcg gcttagcact taagctgagt cagtacaccg gatcgaactg
     4201 caattacctc agccataatg cttattgcga tttcttccgg cgtttctgct ccaattccaa
     4261 ggccaatggg agaatgcact cgcgaaagat cgatgtctga gaagccttct tcgcgaagcg
     4321 ccgcaaagat gccatcgcgt ttggtcttag aaccaataag accaatatag cctgcttttg
     4381 ttcgcaaaag acatgatagc atttccttgt cgatatcggg ctttcgggtg agtatcatag
     4441 cgcaatcctg agggccaatt tcgatggccg gtgcatctct agatgctgca agggggtctg
     4501 caaagctatc tattatcctg agcatgctgg ctttaggaaa tcgttctctt gtgagaagtt
     4561 ccggtctgtc gtcggttatg actacctgaa aaccaactag gcttgccgct acacttgtgg
     4621 ccttgccaac atggccagcc ccgaatatgt aggcgacagg atctggcctg aatccgagtt
     4681 ggaaaactcg agccatattg attttactag cttcaggtaa ttctaattcg aaacaagagg
     4741 caccgcagaa agcttgagcg ctttttagac cagtagtgtc gctccgacaa gaggcactac
     4801 acagactaga agagctactc agaccagaag agccatttaa gtcaggttgt aattttttaa
     4861 tatcctccac tatatttttg gctataagct cttggatttc tgtttgcaga tgaggataga
     4921 ggacatttcc tttctcatct attgcaaagc aatgcttctc ctcgggggcc cttgcaacaa
     4981 gaaaagagca tgcactgcca gattccaggc gctcaaagca ggctttgagc gcattgcatg
     5041 cttcgctctg tggatcaact ctttctaaat gtaaagagac tttgcctccg caggtcatct
     5101 gattttgctg cgcctcaggc gaaagatcga agaatagatc ttggtgatcg ccgccaatct
     5161 caagtagttt tcgtatcgca ataatggctt ggttttctac atatccgccg ccaaccgtgc
     5221 cctccattct gccattcata tccacggcca taagagcatc cgtgcctctt gctgtcgagc
     5281 cgcttgcatg agtcacagcc acaagaacga cagtatcatt gctattaagg cgttcaataa
     5341 tccaggatct aagttcgttc atagatctaa ttggcacctt ctgtttttgc tttatcgatt
     5401 gccataagca cgcgctccgg tgtgaagggg aaatctgtca gacgaatacc gatggcattt
     5461 cgtatggcgt tactgatagc tgcaggaggt gtatctattc ctatttcgcc ggcggatttt
     5521 gcaccaaagg gcccagaggg ctcgtaacta tctaccagtt cgactgtgat tcttcccaca
     5581 tcttcccttg aaggtattgc ataagtcata aggttatggc taagcatatg gcctctctcc
     5641 gaatagcgca catcttcata aagggcaaga ccaattccct gaagggcagc cccttctatt
     5701 tgaatggttg caagattggg atttattgga gtacctacat ctgctaccga tacatagtga
     5761 acgacctcga ctttgcctgt ttccttgtcg agctctattt cgacaaagcc tgctagatag
     5821 ggaggagggg attttgcacc agtgaaggat tccgttgctt cgatagtctt cattctttct
     5881 ccatcgtggt agagggtgtc gaagctaaaa tcgtagagcg aaagcgattt gctgccattg
     5941 agagtcttaa aggtcttgtt gtcgaatatg acaaaagcag gatcgacgcc aaacttctga
     6001 gcgactgctt ctatgagttt tttcttgagg ctacgagccg ctttgactac agcggaacct
     6061 gagacatagg tggtgctcga tgcataagcg cctacatcaa agggtgtatt gtcggtgtcg
     6121 gaagagtaaa ctacaatgct ttctacatcg acgccaagct cctcagccgc gatctgggca
     6181 agaatcgtat cagatcccgt gccaagatcc gtggctccta cgtggagctt aaagaatcct
     6241 ccatcttgga attctattct cgcagaaccc atatcgatga gaggtaatcc cgatccctgc
     6301 atggctatgg ccatacccat tgctcgcact tttcctggag cgacctccca aacgagtcga
     6361 tctgggttcc agccgatgag ttccttccca cgagctatac attcctcgag cttgcaggat
     6421 tcgatggtca tggcgacgcc ttcggtaccc tctcccatga tacgaaatac atcgctggtt
     6481 tcgccttcgt gaatcatgtt cttgcggcga agttccacag ggtcaatgcc aattttatgt
     6541 gcaagctcat ccatagcgga ttcgagacct gtaaggcctt gaattgctcc ataacccctg
     6601 aaggctcctg cggatacctt atttgtgtac acgacatggc caccaaatcg aacggcattt
     6661 actttgttat agagtggcag ggtttttgag ccagcgacca tgaagactgt tagcgcatgc
     6721 tcgccataag caccggtatt agagagcact tgcatgtcga tggcattgag ctttccatcg
     6781 gaattagcgc cgagccgcac tttgacgcgc atttcgtggc gggtaaaggt ggcctcgaag
     6841 acttcgcggc gcgtgagggc gaggcgcgca ggttttccgg tcttcaaggt taccatggct
     6901 acataaggct cgatgtgaat atgctgtttg cctccaaagc ctccgcctat cctgggtttt
     6961 ataatacgaa tctgtcgcag aggcatctga agagctaagg cgagtagcct tcgtgtatgc
     7021 caggggtttt gggtagaggt gacaattacg agccttccat ggatatcaat atagctaaag
     7081 gctgtatgag gctcgagcgc tacatgctgt tgagcttgag tgtgtaccgt agtctccact
     7141 tggacgggcg atttggccaa ttcttgctct acatttccta tctccatgtt gtaggctgca
     7201 gcgatattcc tcttaggttc gaatcctatg gggaacatct catgaatttc gggttcagga
     7261 tgtattacga taggattgtc cagagccttt tcgaaatcca aaacaggctg caaaacctca
     7321 tattgcactt tgacgagcat cgcagctttt tctgctgtag cttcgtcttc tgctgccacc
     7381 actgcaacct catctcccac atagcgcaca tattcatcga gaataaatcg gtcatgaggg
     7441 ctgggctctg ggtttccctg accagctctc gtgataggca cacgtgggca atccttccat
     7501 gtcaggacac aggctacgcc aggcaacgca agcgcttcgg aagggtctat cgatattatt
     7561 ctagcaaaag catgcgggct tctaacaagc ttgacataga gagcattggc tggggcaaga
     7621 tcatcggcaa aggctggtct accgagcacc aaaccttccc catcgatctt tgctatatcg
     7681 tgagcgataa cattgaattt ttgggttgct ttgagcgttt tactatccgc gggcggtatt
     7741 gaatcaaaat tatcaatcat ggactgcctc cagatatttc tttatcgctc ggatctgtcc
     7801 ttcatagcct gagcaacggc atagattgcc cgaaagataa tgacggattt ccgcttcact
     7861 gggatttctg agctcttttt tcatcgataa tacggtgaga gcgaaaccag gcgcacaaaa
     7921 gccacattga tctgctcctt ccgcagtcaa aaattgcgca aatttcttag cttcttctgc
     7981 aacaccctgt attgtggtta ttcgctttcc atcagctcgt acagctagat atgagcagga
     8041 gagtacaggc ttgtcctcga tcagaacagt acaagcacca caacttgccg tatcacaacc
     8101 tttctttact tccacatatc cggcttttcg gagcacttct gtcagcatat cgccaggctg
     8161 gcattcaagg atttctcttt tgccatttat gtatattgtt atggtcatcc ccgcacctcc
     8221 aaaagcgcac gtctgacaag tacaggaagg attttttgtc gataggtagc gcttgctctg
     8281 atatcgctac cgaactgtag ttctttactg ctctcttggg ctgctgcttc tatctgttcg
     8341 ttcgacggat ttgcggaatc agccaatatt ttcattgcgt ttctgcaaag gcgagcagct
     8401 gctggtctag agccggctgc aacacgccat tctgttccca tccaagcaac ggcggtattg
     8461 attataggaa aatcattctc agatatcctc atctgttgat aggcagctct tgcagaggat
     8521 ggcaaaatta tcttttctat taagaaagga ccggcatacc ctttctcgat gaagctagca
     8581 agatcttctt ctatatttgg atagaaaact acacgagctc caagcgcgca gagtgtggtg
     8641 ttgagatcag agaatccata acgtccagca actgttcccc cgaccgttat tatgctccga
     8701 agctgaatac cgacgatatg gccgactgct gtgctgaaga tcttcccgaa tcgcgaggca
     8761 agcaagaggc ttgtttccaa ttctcgataa gttgtcattg caccaatttc gatgtggtct
     8821 tggaattcgc ggatgtattc cagccctaga tcggataggt ccaccccaag cattacagtt
     8881 ttagtatttg ttcgaagcca agcggaaccg ccaatcgcgg taccgtgttc ttcgtgtatg
     8941 agatgcatgg cttcatccat gctcttgggg cgagcatact ctcgtatttc catctgtaag
     9001 ctccttgaaa gaaagaataa ttcaattaac ccctgatttt tctatcatag cataggttta
     9061 gagaatcggc aaattacgaa attctatgaa ttgttgagcc cttagggata ggggacggtt
     9121 cagcaagagc ctaagcttgg ctatactagg agcatggctg aatttgtaca cttacacaat
     9181 cataccgact attcgcttct cgatggcgct gcttcgattc cgaagctaat aagaaaagcc
     9241 aagtctctag gaatgccagg cattgctata accgatcatg gcaatatgtt tggcgcaatg
     9301 tccttcgaaa aagagtgcag agattttggc ctcaatccta tcattggctg cgaattctat
     9361 gtagcaggcg gttctaggtt cgaaaaaacc ggaatggaga atggtaacaa atactcgcat
     9421 ctcatcttga tagctgaaaa cagtattgga tacagaaatc tattaaaatt gacttcagct
     9481 tcttacaccg aaggcttcta ttataaaccg cgaatagatg atgaacttat cgttgccaat
     9541 gcaaaggggc tcatcgcttc aaccgcatgt cttgcaggcg agatcccatc gcttattctt
     9601 tcaggaaagc tcgatcaagc tacaaagaaa attagtttct acaaagaatt attcggagaa
     9661 gatcatttct acctcgaact acaggatcat ggcttagatg aacaaaaagt ggtcaataga
     9721 gagctagtta ggctttcgaa aaagcttgat ctgcccctcc ttgctaccaa tgatatacat
     9781 tatgttgaag aaggggacgc tatagcccac gatattcttc tctgcatcgg tacaaatcga
     9841 aaacgcgacg attcatcgag aatgcgtttt ccaaacgatc agttttattt aaaaagtacg
     9901 gaggagatgg catcgatttt tgccgaggtc cccgaagcca ttgtcaacac gcttaaaata
     9961 aacgagatgg cagctctcaa gatagagttt ccaggaccgc ttctgcctga ctatcaagtg
    10021 cccgaagaat tctcgtctcc cgaagagtat ctgcgccata tagctcgacg aggttttcag
    10081 agtcgttatc ccaatccgga tgccgaggta atccaaagac tcgaatacga gctcgatata
    10141 attataaaaa tgggatttac tggttatttt cttatagtat gggattttat caattgggcc
    10201 aaagagcatg gcattcccgt aggaccgggt cgtggttcag gtgccggatc tatcgtagct
    10261 tactcgatgc ggatcaccga tatcgatcct ttgaagtatg atctgctttt cgagcgcttc
    10321 cttaatccag agcgagtgtc tatgcctgac tttgatgtgg actttgactt cgaacggcgc
    10381 ggcgaggtga tcgattatgt tactcagaaa tatggtagcg atcgtgtcgc ccagatcatt
    10441 acattcggta cgctcaaagc aaaagctgtt attaaggacg tagctcgtgc cttagatatt
    10501 cccttcgatg aagctaatca gattgcaaaa cttgtgccag aagatccaaa aatgactctg
    10561 cataaggcta tcaagatgga gccaaagctt gctgctcttg aacataatcc ttcttattca
    10621 gagctttttt cgatagccgc aaagctcgaa aaccttcata ggcatagttc gatccatgca
    10681 gctggtatag tgatcggaaa gacagcccta atagactatg ttcctttata taaggatcca
    10741 aagactgggc ttattgcgac tcaatataca atggagtacc tcgaaggctg cggccttgta
    10801 aagatggatt ttcttggcct caagacccta actttaattc gcaataccat ggagcttttg
    10861 agaaaaagag gcatatactt aaaagaggaa gatatcccag aggacgattc caagaccttc
    10921 aagatgctca gcgaaggtaa gagcacttcg atttttcaat tcgaatcgca gggaatgcaa
    10981 tctatcctaa agcgagccaa gccttctagt attgaagatc ttatcgctct caatgcactc
    11041 tatagacccg gacctatgga attcatagat cagtatatag attcaaaaaa cggtaaaatt
    11101 gctatcagtt atccccatcc ttcgctcgaa aaatacctta aaggcaccta tggagtgatc
    11161 gtctatcaag agcaggtgat gcaggtcgca agggaagtag caggatattc gttgggacgc
    11221 gcagatcttt tcagaagagc catggggaag aaaaagcccg aaatacttca aaaagaagaa
    11281 gaaccattca tcgaaggagc tcttgctcgc ggctattcaa gagaagaagc aaagcgcatt
    11341 ttcgatattc tgacaccttt cgctggctat ggcttcaata agtcgcatgc tgccgcctac
    11401 tctgtaattg cataccgtac agcatatcta aaagcaaatt attctgctga gtttatggca
    11461 gcgaacctta caaacgaaat cagcaacacc gataagctaa ctgagtacat aagtgaagct
    11521 cggcagatgg gtttatcggt gttgcctccc gatatcaatc gttcagacgc caagttcact
    11581 gtcgacaatg ggcagattat atatgggttt cttggcatca aaggcgttgg tgaggggatc
    11641 gccatggata ttcagaaaga aagggagtct catggtaaat tcaaggattt tcttgatttt
    11701 cttacgcgca tgaatgatgc gggcataaat cgcaagacga tggagtcgct cattatggca
    11761 ggctgcttcg attcctttgg aaagggccga catgagctta tggagaactt tgagcgcgct
    11821 atgaattatg tacatgagaa attgagtttc gaagcttcgc gctcaggtat gcttttccag
    11881 gaggaagatc tgggaatcta tccagatttc gattttctac ccttcgaaga attcccgaat
    11941 caggaaatgt tgcgagcaga aaaggagtta ttgggatttt tcttttcagc acatcccatg
    12001 gatgaataca agatgatctg ggagagatca tcgacacttg atcttggaaa tttggagaaa
    12061 gaaagtgtaa accatgaata tgtagtagtt gcgcttctca aggagtttcg cagctataca
    12121 acccagaatg gacgtcgaat ggcctttggt aagcttgagg actacaatgg ttccatagaa
    12181 atagtcgtgt tcccggatct gtttgagaaa aatgaggcgt cctttgtgaa agatcgtgtc
    12241 ctgtgccttc agggagtact ggacaactcc agaagaggtt tttcgttcaa agtgcaagct
    12301 gttctggatc cgacttctct taagagcaat tcttggagag aattgcacat taggcttacg
    12361 gatgctgcta tcgacgaaac tgctctctat ccactcaggg atgcgctata ttctatacaa
    12421 ggaaaatgcc gggtaatctt tcatgtgcct ctaaaggggg gaggggaggc tctaatcgaa
    12481 gctggggcta gtacaaggtg ctctgcgagt gatgaggatt tggcgtttat agaaaggcaa
    12541 gctgcagtga cggaggtatg gcgcgaatga acataaaaat acttggcaat ggcggcttct
    12601 acaatgaggg attggcttat aatgcgctcg ctatcgatgg ccatgtgctt gtggagactc
    12661 ctccagacat tctccaatct cttcatagcc agggaatgaa gccttctcag atagatacag
    12721 tctatattag ccacaatcac ggagatcact gttttggctt tccatttttc tttttcaatt
    12781 ggctatatgc ggaaggctca gattctcata atgaaaaaca ggcagagcta gtcataatgg
    12841 ggcccaaagg actaaaagaa catctcggct ctctcatgcg ccttgcaatt ctgcctgaac
    12901 accaatattt gtccgaattc gaccaaagag taaggtttat agaagtcgac gaaaaaactg
    12961 tcttacctgt aaagggaggt ctttggtttg gattcttgcg tacgaagcat tcggttccta
    13021 ccttttcact tatagttggc aaagcagggc atgagaatga gcttccttct tgttgcgatt
    13081 accttgagaa agcgctattt atatatagct cagatacttc tatgttcgat ggtatatata
    13141 agctattaaa ctcaagcgca aagcttatcc tctgtgacac gaatggtgaa gaggaaaatg
    13201 gcgttcatat gagtccgaga gaacttatgg cagcaaagag tcacattagg agtaggagcg
    13261 aggaatgtgc ggttctttca gagcaaagac agagcgtggg aaatgacata gatcgcgtct
    13321 tgggaattca tctaagcagg aagatggata gggttggaca acttagattc gcccagcctg
    13381 gagaagaata ctgcattaag tagctatttc caagagggat ctttaagttc ttaataggaa
    13441 tgagccaata cccactgcgt aagttcttcc caattagaaa atgaaagatc tgcagctgag
    13501 ttttgtcgcc ctcgtaaggc tgctcgcaag cctgcttttt tagcgccaag gatatcgtac
    13561 tctatattat ttccaacata gagagtctcg aaaggctgaa cacgaatagt tttaaccata
    13621 ccatcgaagg cgcgctggtg gggttttagc acaccccatt cttctgtgca gagtttgtag
    13681 tcgaatagat catgaagacc aaggaattgc aatttctctt gtggaggcaa atcggaaaga
    13741 gcagctatta tcaatccgtt ctgtcgtatc gcctcgatag cttgccttgt ccctctataa
    13801 ggatggatcg atgagaaacg ctccggcaca agatgataga aaattttctc tatggattgt
    13861 tcgatgtgcg actcgtccat ttttagacgc tttgcaagaa gcgagacctg caaactatga
    13921 agctcctcca tatttcgagg aatatagtta ggttgcgctt gcagaaatct gatttctttt
    13981 cttacagttg aaaaggccat aaatatgcga ggttgaccaa agaataagtc aacacatctc
    14041 agagcgattg aagattccgg gtaaagagta ccatcgacat cgaatgccac tgcgcgcagg
    14101 cgagaccgtc cttccataaa caactgtata tatgaagcaa aggagggctg tctatggcct
    14161 attcaagcac taaggcaacc gcagtggctt ttgaaaataa tagaatgcct taaaaacctc
    14221 agaaaaccct cgccttatag ctagtatgca atagttcatg agaaaggtgg ctaagcggct
    14281 tgcccagaaa ttcctcatag agtgttgtta tagcaggatt ttcgtgagat tttctaattg
    14341 gaagtctgcg atcctcttcg tagagcgaca ggctacgagc ttttttcttt tctatgtctg
    14401 caagtactgg ctgtccacct ccaccaacac atcctccggg acatgacatg atttcgatga
    14461 aatgatagcg actttttcct gctcgaatct catccagcaa aatacgcgca ttggaaaggc
    14521 catgagcaac agctacgcga acaggagagc ctccaagcaa gacttcggca ctctttatcg
    14581 aatcgaatcc acgcacttgg ataaaatcca agctctctag aggtttcttt tccacaatct
    14641 catagacagt gcgcagagca gcttccatta cacccccggt cgttccaaaa atagtggccg
    14701 caccggtgga tttgcccaag ggatcgtcga aatcctcttc cggtaaggct gtgatatcta
    14761 taccagcaag ccttatcatg cgtgcaagtt ctctagtggt gagagcccaa tcgacatcga
    14821 agaatggtcg caagccggtg ctttccggat ctttgtcttt ccaccaatcc caagcagaaa
    14881 tcatttcttt gcgtctagct tcatatttct tcgctgtaca aggcattatg gagaccactc
    14941 tcatattctt gggtgcaatg ttggcatttt ttgcataata tgtctttgcg acggcgccaa
    15001 acatctgctg tgggcttttg caagtggaaa catggtcgag cagatcagga tagaaacctt
    15061 cgataaaatt tatccatcct ggtgagcacg aagttatcat gggcaaagtt ccgccatggg
    15121 aaagccgttc cagcagttcg tttccttctt cgattatagt gagatcggct gcgaactgcg
    15181 tatcgaatac ttttgagaat ccgagcctac gaagagccgc caccatttgg ccagttacaa
    15241 ggcttccagc aggaagacca agagcttctc caagcgatgc gcgaattgca ggagcggtct
    15301 gaactaccac tgaaagccta gaatctccaa gcgcgccata gacatcctgc gattcgtcct
    15361 tttcggttat ggctccggtc gggcatacca aagagcattg gccgcactgt acgcaagcgc
    15421 tttgagccaa tcctctatcc ataaaggttg caactctagt tcgagtaccg cgtcctgtaa
    15481 aggcgatagc acttacaccc tgggtttctt cacagacaga tacacagcgt ccgcagagta
    15541 tgcattttct atcgtcgcgt attatgcctt cagaggaatt gtcggcaggc ggatattttt
    15601 tatagcctgg gaatctgtca gcacgaactc caagcaaatt ggccatagtg tgaagctcgc
    15661 aggtatcggc tcttaaacaa gagaggcatt catctgggtg gttggcaagc aatagttcga
    15721 cagctgcttt tcgcgctctc ataacacggg ggctcgatgt ccgaatcttc attcctggtg
    15781 tgggcttttg tacacaagag cgcagaaggg attttgctcc ttctacttcg acaacacaga
    15841 caccacagga ggcattttgg gaaatatcct tgagaaagca gagggttggt atctttacgc
    15901 cggctcgcgc acaagcttct acgatatttt cgtttgaatc gacccttatc gtttgtccat
    15961 cgatcgttat atctatcaag ttttccattg atatgcctcc tttattcgcc tacaaggctg
    16021 ccacgaggtt cgcgtctatg ttccctcaca tcgcagcgaa ggcaacgata agcctcgatt
    16081 cgcgcttgct caccagtgta gccgagattt atctccataa aattgctctt acgtgcattt
    16141 accggcagca tcgttggttg ggtcatcttt tccttactga tattggcagg aatttccatt
    16201 ctgtaatcaa agcgtcggaa tagactgtcg aagcgctttt tcccgcttaa tgcctgatcg
    16261 attatctcgg caacggtttt tgcctgtccc attgcctctg ctgccgtcgc tggaccgagc
    16321 attgcgtctc caattgcata taccttgggg ttagatgtta tatgagagaa ggggtcagcc
    16381 ttgatgcgtc cattcttgag acgttctatg cctagcgctt ctataccagg tatctccact
    16441 ttttcgccta ttgccataat caccatagag caaggaatat cctcgatttt atctgtagga
    16501 acaggtgttg gccgtcctgt agggtcgata ggaccggctt tcatacgctg aattctgact
    16561 gcttttgcaa cgcgcttgcc ttgctgctcc tgcgttacga tttcaagggg cgaagccata
    16621 cagataagct ctacgccttc ctcaagggca ccctttagtt catccaggtt ggctggcata
    16681 tcggctttcg atcttcgata aacgattctg accgtcgcgc caaggcggag agcggagcga
    16741 gcggcatcta ttgcagaatt tcctccgcct attaccacta cttcgccgcc tgtatgaact
    16801 ttttcttttt ttgcgagcgc gttgaggaaa tcgcaggctt tgtagacacc tagagcgtcc
    16861 tcccccggta tgttcatgga aagttcggaa gaagcgccag cagctattat tattgcgtca
    16921 ttctcatcgg caagcttctt ccactgttcc ggttcggtga tgcgcgtatt gtatacgaag
    16981 cgtgttccca gcttttcgat gagattgatt tcttttttga ggatgtcttt tggcaatcta
    17041 tacgatggaa taccccagcg aagtacaccg ccagcctcct gcctggattc atagatggtg
    17101 acttcatgcc caaggcgcac taagtagaaa gctgcagtca ggccagccgg tcctgcgcct
    17161 attatggcaa ttttcttgcc gcttgggggc agtttttctt tgagaagctt tgtatatacc
    17221 tgcttttctc tgcccatctt gtacattgtg tcggcaagat agcggtggat ctctccctgt
    17281 gataccggtt catccagcat gtcacgtcga cagcgcatcc ggcaatggaa gtggcagatt
    17341 cggcctattg tgccaggtag aggattttca cgtagagtga gctcgaaagc atcttctatt
    17401 ctgttttctt ttagaagctg caggtatcct ggaatgttca tgtgcaaagg acaggaggat
    17461 tcgcaaagag cgatgtaaag gttttcgcaa acgccagccg gacagcgttt tccctttata
    17521 tgctctatat attcatcgcg gaaatagcgc agagttgtaa taacggggtt tgcagaggtc
    17581 tgcccaagac cgcagagaga agaatctttg acaatcgttg cgatttcctc gagcgtatcg
    17641 agatcttcct cactcgcttc gccatcggat accttttcga gaatatgcag catttgggaa
    17701 gtaccttctc ggcatggcgt acatttcccg caggattctc ccattacaaa gcccgtaaaa
    17761 tatctagcca catcgaccat gcaattatct tgatccatga ccaccatgcc gcctgatccc
    17821 ataatggctc ccagctcagt aagatgttcg taatcgatag gggtcttgaa gaggcttgca
    17881 ggcacacagc cgcccgatgg tccaccgcat tgcacggcct ttatttttct cttaggaccc
    17941 atgcctccgc atataccata gattacactt tcaagcgtgc tgccaagagg aagttctaca
    18001 agcccagtat tacgaacctt tccgacaaag gaaaacacct ttgttcccgt gctcatagga
    18061 gtaccaaaac cagcaaacca ttctcctccc ctagctatta tgactgggat attgcaccat
    18121 gtttcgacat tgttgatcga ggtcgggcga ccatagaggc ctttctctac agggaatggg
    18181 ggtcttggtc tcggccttcc ggcatttcct tcaatagagg caattagggc ggtctcttca
    18241 ccgcatacaa aggctcctgc tccagtcacc atatctatgt cgaacgagaa cttacttccc
    18301 aaaatgtttc ttccaagaag tccatgctgt cttgcagatt ctatggccaa attgagcctg
    18361 tagacagcca atggatattc cgcacggaca tagacaattc ctttgtttac attcatggca
    18421 taagcaccga gaagcatccc ttccaatagc atatgaggat cggattcgat ctcatttcta
    18481 ttcatgtagg cgcccggatc tccttcatcg gcattgcaga tcagataggc ttccccagga
    18541 ttatcgatca gcgacttacg cattagcgac cactttttcc aggttggaaa gcctgcaccg
    18601 cctcgtccac gtagtccaga tttttttact tcttcgatga tcgcctctgg tggcatagaa
    18661 gttagagctc gaacaagact tccgtatcct cccaccgcga tatattcttc aatgctttcc
    18721 ggatctatca ttccagcatc gcggagaata atcttttctt gatccctgaa gaagttgagt
    18781 tctttccatg taggaaggtt atcatagcct ctgccaaaat ctaccttaga tgttctgaag
    18841 tcccattctt cgattttggc ttctgcaatc tttgcgattt tatcgtatgt agcatctgat
    18901 gaaagaccag cgaggatgcg tgatgctttt tgagtattga cttccgtaaa gaaaagaatt
    18961 ggcttgccag gtctccaggc cattacaacg ggttcggctg cacagaaacc aaagcaacca
    19021 acacggcgca ggtggatatt ggtattctct gtcttctgag agagaatttc ccaaacttta
    19081 tctgctcccg agcctatgcc acaggttccc atccccacag aaatccaagg agtatttcca
    19141 tgaaaatggc tcgagccttc ttgccctaga aatcctagat cggtgttgtt gatcatcgct
    19201 cccctccctt tttaagctta tccagaagag agacgagctt actgacagtc attcgcgata
    19261 gcactaagcc atcaaccatt ataacaggtg caagagcaca ttggccgaag cacgctactg
    19321 tgcgaatcgt gattagatta tcactgctcg taaccgaatt ttcttctttt tcattgaatt
    19381 ctttaatttt gagtctagaa agggcttcgt ttaacagcac gagcgagcct cttgtgtgac
    19441 atgctgtacc gcggcagaca attaataaat gctcgccctg cggcttcaaa ctgaagaaag
    19501 aataaaatgt cgcaactgaa tatactctcg agagaggtat atcgagcctt ttcgagattt
    19561 cccttagagt tgtctcggag agatattttt taggatcgtt ttgttgtgcc tgttccaggg
    19621 ctccaagcag gcttccattt ctacctttcc atgaatcgat gatgctttcg acattcaggg
    19681 gttctgaatt ggagattgtt tttgtcatgc tgcctccttt aactatcttt attattttat
    19741 atcgattagg atatttgtca agaaaaattt atctataaat tcctaattaa aaaggcagca
    19801 aaagcggaat caaaacgaat agatcttaat aaatagttaa atatcgataa aaagagtatt
    19861 gcatctaggc taaatttata caatagtata gatatggctt tgaatgaccg agaaaatgaa
    19921 gagatttcta ttactcgtcc caaatggcca atcaaattca ctaaccagag cgtggattgc
    19981 gtcggtcgaa aattgaaacg tatcttcgat cagaatgcca gttcagacta cttcactagc
    20041 gatgagatcc gcgactattc ctactacgaa aggcttctgc tacaagaact tgaaggcatg
    20101 gaccgaaaaa tatctaaagg aaagctaaag ccaagccata ttgtggaata tccaagggtc
    20161 attcatgcag acgaaggcaa aattcgtgcc tgcatataca ttggttcctt tgatcctttt
    20221 cagcttaccc atctcaccgt tgctatgcgt tttcttgcct ctgatctaag caattcagat
    20281 ttcgtaatta tagtacccga aggttcgcct gatacagcaa agccccttaa aaccgattat
    20341 gcttttcgct attcgatagc gaagatgcag atagagggca ttttcgattc ttttattaag
    20401 gtattagatt taggtacgca agctgataca atcgagatta taagaagatt catagcaatg
    20461 catagcggat tcactctcga gttgacgcac ctaataggtt cggattcgct gccgatagct
    20521 gcaagcttga tatcgcaaga tatgcgaata tggagaaaag aagctcagga atcaaaagta
    20581 gattatcatc attctctata tgttattcag cgaggggatg taccagagat acgcccctat
    20641 ttagaggcct ttgaagagga aggtatccaa gtggttatcg atcctttcag cgtggaagcc
    20701 ccatcgagca gcgattttcg tacaaaacag gcatatacga tagtactgcc gactaattcc
    20761 ataagagaaa aaatggaaat aatctttcgc tatcacatgc acaaaacttg gtcttcagac
    20821 cgcaattaga tgtagaggat tctatttgat ttagatgtag atatccaaaa gcgccacaag
    20881 ccctttttta gaccttcgaa gtagtgtata agtattttat gaatattcag tctatgaaat
    20941 tgattttttt agggcctcca ggagcaggca aggggacgat tgcaagaatg gcctcggaga
    21001 ggctgggtat tcctcagata agcacgggcg atatatttcg tactgcggta aaagaaaaga
    21061 ccgaactagg tctaaaggta caatcgattc ttgccgccgg cgctctagtg cccgatgagc
    21121 tgacaatcgc catagtaaaa gaaaggctat cttggccgga cgtaaggcaa agctatatcc
    21181 tagatggctt tccaaggact attgctcaag cagaagcgct agcctctttt tcaaaaccag
    21241 attctgcggt ggactttgaa gtcgatgatg atttaattct ttttcggctt acgggaaggc
    21301 gagtgtgtaa gagttgtggc gcaatctatc atatagtaac aaaaccacca gcaactgaag
    21361 ggatttgtga tatatgtgga ggcgagctat atataaggaa cgatgatagg gaagaaaccg
    21421 tcaagattcg actaaaagca tatcacgaag aatcagaacc cttgatcgaa tattattcta
    21481 ggcaaggaat tctaatgact attgacggta gtcctgatcc agaaactgtc tatgatactt
    21541 ttatttcaaa gattgggcga ttcttgaaga aatgagtgtt ttgagagctt tgttccataa
    21601 aaaagtcgaa ttttttccga gtctcttttt tttgcttatg atcttgacga ttgtctcggt
    21661 cttgcctaat ggaatcgcag gtgcagaaga gatagtgcca ggggtttgga aaggcgtatt
    21721 gttattgcga gaggtcggcc agaaacaatc ttcggcacaa cttccttcgt attctatgga
    21781 tcagcaatca acgccattta gtttcgagtt ttatttaagg attctctcta aggagagggg
    21841 attattggta gatattcctg aacaaggttt atattcttat cctatagatg aatatacctt
    21901 cgatgcccaa cagataagct tcattttcga cgcaatagag ggtgatgaag agcttcgctt
    21961 tttgggtaat tactcaacat cttttctacc tcctgggagc actcacagag gagctatcgt
    22021 tggctcagtc agaggccgat cctggaatgg atcattttat gtgcagaagc aagattcgtc
    22081 gacggatcca ggtcagatta gtctagacat ccctgtggag ggtggagcac tacctgctac
    22141 tcttgtcttt cctttgaggg agctaccttt cctcgatccg aattctccac ctgattttcc
    22201 tctcatattg cttgtagctg gtgctggtag ggcagacaga gatggaaaca atgtcgatgt
    22261 tcccggaaag atcgattctc taagacagct atctaatatg cttagaatgc gcaatgttgg
    22321 aactcttcgc tatgacaagc gaggcacggg cgaagcatac aagctcgaaa aacctggtcg
    22381 catgactagc ttttccgagc atgtcagaga tctaacttct gtcattattg cagctgctgc
    22441 tctacctcga catggaaggc tgatagttgc aggcatgaac gagggagcct ggattgccat
    22501 gtcagctttg gaagacgaaa aggttgcctc acttgtcgat ggttttattg taatagatgc
    22561 tagcggtcaa agtccaatag agaccttgaa acagtctatt gcaagcctag atgaagagct
    22621 tcgagcaaaa gcattggaag cagcgcgaaa tctagtccaa aggggaattc tgatcgatgt
    22681 cccggtagcg cttgccgatt tttttgcacc gagtagaaaa gattggttgg caacttggct
    22741 tgcctttgat cctgtcgaaa cgatcaagca agtccaactt ccaattctgc tcatttatgg
    22801 agaaaatgat cttcagataa gtcgggagga gttctccatg cttgttgaag cgaggcccga
    22861 agctgcagtg cgaattgtac cagagatgaa ctatgtgctc aaagaagtta aaaacgaaga
    22921 tgaaaactat gccgccttca ctgacccatc tttcaaagtg ccttcgctcc tcgcagacct
    22981 gcttgcttca ttcgccaagg ctcagccagc acctgagggc tttgtaccct ggcgattatg
    23041 acactaattg ttgaaactcg agatagaaag agcaacatct acaagttgct gtgcactgat
    23101 ccaaacaggt tggctaacga aaaccgatcc ttgttgctcg attggggtct ctggagtaat
    23161 tggaataccg taagagccta acttggaaga aagcataacc caaagaaaac gaacgatagg
    23221 cttatatgtg cgcgcacctt gctcatcctt catttgtatc ttaaaaatgg tacgatacat
    23281 agcagaagga ctgccttttg gaggacttgt tgcttcccgg gtcgcactca tcaaaatacc
    23341 ctcaaaaggc aaagtgaccc cttccataat aggcaactga ctgacgattg tagattttgc
    23401 atcgatatag attgcaaaat caacggcatt tgcgagcact ccgctcttgg tagctagtgc
    23461 agttgctcca ggattagtga gaggagaaac agggggcatc catgttgcaa ggtgaacttg
    23521 gcccctgtct ggatttcgga catatagact tcctgatttt agagcataac cgccattgat
    23581 ctttttccag ttagaatcaa ggctaaaaga gagagaagtc aaaagatctg gaaaattccc
    23641 tgcaaataca gcttctaaag ccggttggcc cttatcggtg agcgagattc ctattcctgc
    23701 aacttgcgct ctagtcaaaa gtttgtcgat tttagcccga tcgattggct ttgaaggttc
    23761 tttcatttct tccgattcat tcggtttatc agaagcaata gcttcaagag cctcgattgt
    23821 cgaatcatct aatgatgcgg cgatatcgcg aagaaaaata tctcctgaac gcagaataac
    23881 ctgtggagtt ttttcaaaat gatcgaatat ccctgcaata gctatctctt tgggcacaga
    23941 ggagcaggag acaatataaa tgcaaattgt tgccaagata ataatacttg caatcggtgt
    24001 attttttgct cttttctctt ttctattatt tggtaaaaac cccataaaga ctgaattcct
    24061 ttcttgtttt ttaggtaagc taagcagaat atatcacgga atcctctaga atagcgagca
    24121 aggaaatgga ggagcgacta atttatcgaa ctcccgatgg tttgaagcta gaatcaatta
    24181 agctatatct gtatctttgt gctgacattg tgattcaaaa catgttattt tggatattga
    24241 atctatattt tagctcgtaa aatagcacat ggaggtttgg aatatggcca ctgagcgaac
    24301 atttgcaatg ctcaagcctg gtgtcttgtc ccggcgcgtt gttggagaga taatctcgaa
    24361 gatcgaacga aagggcttta aaatcatagg tttgaaaatg attagtgtca ctagagacct
    24421 tgctgaaaga caatatgctg aacacaaggg gaagcctttt tatttagatc ttttggacta
    24481 tattacgtcg ggacctgtct tggtaatggt tctcgaagcg gatcaagcaa ttaagctttt
    24541 caggcttttg tgcggtttga caagtcccga ccaagccctt cctggtacta tcagaggaga
    24601 ttatgcattg cacaccagta ccaatatcgt ccatgcctca gattcgaagg aatctgctgc
    24661 gcgggaaatt ggcctttttt tcaaaccaga agagataatc gattgggaag atgccaatca
    24721 aaaatggatc taaggtaaga gaaagtcaat tataagaatg attatatctt taagatgcga
    24781 ctatcaaaga agggggtctt atgtcaaaga cggctgaaat accgtgctgg aatcttgatg
    24841 atgtttttca tggctttgaa tccgaagaat acaaaaaggc aaaaaacgag ctcaaaaccc
    24901 tactggaaaa cctggggatc gagcttgaga atgccaaacc accttccgat ataaaaagca
    24961 cggccaactg gctattcgag cttctaggca agttggatcg gctcgagacg cttgcagata
    25021 cactatctgc ttatgtccag gcacgtttta gcactgatac acgggatgcg ctcgtgcttc
    25081 aggagcttgg aaaggtagag gagcttcttg tccccgccac aaagatccta gtgctcttcc
    25141 gcaatctgct tgctcgctat gaaaagagga ttaagaaagt catatcacag gacagcagat
    25201 tcgtagacta tgcttttgta ctggaagagg cgctaatgtt ccaaaagcat cagatgttac
    25261 ctcaacttga agatctggct gcggatcttg gtcgctcagg ggccgatgct tggtctaggc
    25321 ttcaggattc gatacttgca aatgctagcg ctatatggaa tgaagcaaca ggggaacgca
    25381 agacaatggt tgagctccga aacctcgctt atgatgcgga tcgctccatt agagagaaag
    25441 cctatcggct cgagctttct atttggaaaa catacgaaat accagttgca gctgctctca
    25501 atggtgtaaa gggcacggtg ttgacgttga ataaaaggag aggctgggag agcgctatcg
    25561 atgcttctct tgctcaagcc aggatatcta gagcgacact agaagccttg ataggcacta
    25621 tggaggagtc tcttcccatg tggcggcgtt atttgcacgc caaagcacga cttttgggtc
    25681 ttgagcgact ttcattcttc gatctttttg cgcctctaca gaagcaagga gccatgatta
    25741 agagttatag ctggccagaa gccaaagact tcattgttag acagtttggt agttttgatg
    25801 aagaaatggc tcgctttgct gaaaaggcct tcgaaaacag atggatcgac gcacggccac
    25861 gcgaggggaa agttggcggt gcatattgca cacacttccc tgcagccaaa caaccaagag
    25921 tcctctgcaa tttcgatggc tcttattcct ctcttataac catagcgcac gaattaggcc
    25981 atgcttggca ctacgagaat ataaaggatt tgccaacctt gctttctcag tatccgatga
    26041 cactggccga gaccgcatcg attttttctg aaactttggt ctccaatgcc gctatggctg
    26101 ctatggaaaa ggacgctaga ttacctctga tagagctcca tttgcaggat gcttgtcagg
    26161 ttatagtcga tattctttct cgttattatt tcgagcatga tgtattcgaa gagcgagcaa
    26221 aaggcgagat aacgcccgat cgcctatgcg atctgatgct tcaagcccaa gcgaaaacct
    26281 atggagagcc tctgcgagaa gacgagcgtc atccttatat gtgggcggtc aaaagccatt
    26341 attatattcc tggactttcc ttctacaact ttccatacgc ttttggtcag ctatttggta
    26401 tggggctcta tagcatctat aagaaagaag gccaatcctt cgcagctcag taccgtgagc
    26461 ttttacggtc gacaggttca ttgcctgcag cagaagtagc aaggcgcgca ggcttcgata
    26521 tcgaaaaacc tgcattctgg cagactgcta tgaaggcatt tgaacatgaa gtggaatttc
    26581 tcgaggctca gagctgtaac agatagcctt ccgctagact atcaataggg ctttagaatc
    26641 gataaaacct tttcttttcc cagggttctg aggaagcctg cgagccttgg tccttgatct
    26701 ttgcctatga gagcctgata tgctgcaata aacagctttt taggttctat attgctagct
    26761 tgagccacgg cataaattgc ctcagctatc tcgttttcgg aatcataggc atcgagcctt
    26821 tctataacct cgtcccgcaa gcaccgcaat gcattcgtct ctgcctcggg aagagaagcg
    26881 agttcaccag gcttgcgcag gaagaatctg aagtcttcag gtgcgcattc ggttatccaa
    26941 taccaagcgc attgagccct tcgtctaacg cgtgcttctt gcgaaggctt taatccagga
    27001 agaagcgata gcacagcatc gatgtttcct agatgaatct gcaagagatt gcatagatgc
    27061 cgaaatggta cttgataggg catttcttct ggagttttgc ctacctgaga cagctcatag
    27121 atgcggcgtt cgagcgcttc gacttctttg ttcttcgctt tttcaactcc ccaataaatg
    27181 cgctctgttc tatcgtaatc ctcgtagatc ttaattacat cgagatcgaa acttattaca
    27241 aactctgtat ttgggcgagt tccagcaaag agatagcgca ctatttctgg ctgatatacc
    27301 cgcaataggt cctgaagatc gacaaccttg ccctttgaag aggacatttt gccaggcgag
    27361 cccttaatac cgataaaatc atagcggaag gtcaccggtg gctttctgcc atagacttct
    27421 tcgaccacat gcttcgaggt atcgaaagaa cctccttgag aatgatgatc cttgccagca
    27481 ggctcaaaat cgaccttttc gtaagcccaa cgcataggcc aatcgacgcg ccacacaagc
    27541 ttagctccct ttagcgttcg taggtcgcct ttttctttga aaccgcaggt ttcacaatgg
    27601 tagctcaagc cccattcgcc gtcccattca tcgatctcgg tggtatcgcg gttgcagttc
    27661 gagcagaaca ctgaaacagg ccaccattcg ccctgaatct tatgatcttc atcgcgatat
    27721 ttgtctagga ttgtctttag gtgttcgcgc atctcaagag cacggcgcat gctggatgca
    27781 tatacgcccg agcgatatcg ttgagcttgg tatatgaatt caggatagat tcctacttcg
    27841 ggtagtactt tctctacatc gacctcatgg tgtcgagcat aggattcatc gcgaccccag
    27901 gggtctggca ccatggttat cggaaatctt aggtattttt ctaataattc gggatttggc
    27961 atattgagag gtactttgcg gaagacatcg tagtcatccc agctgtagat gaatctgaca
    28021 tttttgcctc tatctctaag tgctcggact acaagctcta ccgatatcat ttcacgaaaa
    28081 tttccaacat ggacagttcc agatggagta attcccgatg cgcaggtata taggtccttt
    28141 tcgccccatg tatccataat tttttcagct gtttgatcag cccagtgaat agatttgttc
    28201 tgttccatat tgtggaagta taactatatt tacgtttttg tagaatagcg gaagccaatt
    28261 cgaagcctta gtagagctca tattccaatg aacagggctt acaaggctag aacaccttgc
    28321 tccatgatag cgatttcttc accagtaggc attaggagca ccactgtagg gaatcttaca
    28381 agtccatcta ggtgaataag ggctggcgca tcttcgtcat agttcgatcc gattgcaaaa
    28441 tgaattgtac ccattatttt ctcatcttcc aacatattgc ccgaaatatg agcagctggg
    28501 ttgagcccaa tgccaaattc tcctagatgg cgagcatttg taccgtatgc gagcgccatc
    28561 tctggagaca ttccttgctc gcttacaagg tcggaagcca tagaaagccc ttgtatcaga
    28621 gcggtatcta gtcttttcgc ttcgtttccg ccttcgatgc cgataacata accgcctcgt
    28681 acagtgcaaa caatgggctc ctcaatgaca atatctctct ctatatccga aatagaccca
    28741 tcgaacacaa tcgtgccttc tgccgatttc aaagccggag aaataaaaac ctcacctgca
    28801 ggcagattcc cacctttgcc tggatatcga aaatccccgt catccaacat tgccttgcgc
    28861 ccgagtatcg aaaatacaat atcggtgcca ctagggctcc gaacaaggac cgtacttgct
    28921 tgatcgagaa aacttgcgag tagtgctgcg cttctgcgca ttacttcata atctaccgga
    28981 acagttctca gaaatatgtc cttcgtaatc cctggtgtcc agactgcgcg catcgccttt
    29041 acaccatgca agagataatc gaatatgtgg tcataagagc ggccattggg agccaagaga
    29101 ggcgatgcaa gccttgcctt atctttacct agtttttcag tcgaaataga tagtaccaca
    29161 tttgggcttt cttctattgc accgatcacc tcatcttccg caaaatcttt ttgggttttg
    29221 actggctgaa gtaaaaatcg tacttttgct cccattgcgg aagcttgttt tgcaaaagct
    29281 tttgcaatct cttcctgttc agtctcaggg ttggaaacaa tcaaaagcct ttctgattct
    29341 tttacatcga gacactgagt aagtgcaact tgcgcaacct ccgtgagggt atacatcaac
    29401 cgtcctcctg atgccacttg aaatctgcaa aacgagaatt tggagctaaa ttccagatgt
    29461 ctgtctcgcc tcgctgaaaa taatgccatg caataccagc aaccatggct ccattatctc
    29521 cgcagagcga aataggaggg aaatacacat atagatcttg ctttacttcg agttttcgtc
    29581 tcaggtatgc attagctgca acaccgccac ccacaacaac tgtattgata cctgtatcct
    29641 gtactgcacg atagaggcgg gatagtagta tatctattgc tgtttttcta aaacttgcag
    29701 ctatattttc tggcgtaacc tctgccccag gccttagata tgaagaaagg tgatagatga
    29761 ctgccgtctt aagccccgaa taggaaacat cataacgatg ctctccttta tgcagagatg
    29821 gcatggggaa tttgaaggct ttttcatcgc ctttctccgc aagattatca attgcagcac
    29881 cgcctgggta accaaagcca tagtacttag cgactttatc gaaggcctcg cctgctgcat
    29941 cgtcaattgt ccttccaagc acctcaattc tattgaaatc ttctgcacgg cagagaatcg
    30001 tatgcccgcc cgacacaagt agcccaagaa acggatagtc gataggttcg atcagctgag
    30061 gagcatagag atgagcaagc atatgattta cgcccttgaa tggaagcttc atagaccagg
    30121 cgagggcttt tgcaaaggac aatccgacag caagagaacc tataagacca ggcatagcgg
    30181 ttgctgctac gccttctata gaagatggtt cgattccggc ctcgaataga gcgcgcttaa
    30241 ccacgcctgt gatccattcg atgtgtgccc tgctggcaat ttctggtacg acaccctggt
    30301 atcgctcatg gagtggaatt tgagtcatga ttacattgga aagaatatgt cgtccgtctt
    30361 ccacaattgc agcggcgcat tcatcgcagg aagtctcaat ccccaaaatt ttcattgact
    30421 aaattctctt ttgcttggat cttcatgtat tggctgatag tagagagcga atagccttgt
    30481 tccacaagtt caggatagat ctccatcagt gtagatgcta gcttggacga ccatacatgg
    30541 ccaatcatta ctgccgcgcc cttttgccga gcaatctgct ttccttcttc tattgcagca
    30601 agaattgatt cacggtcgct attattatcg agaaacacat cgcgctccat atattgcgtt
    30661 cctatttttt ggctcaactc tgcacacaca gttccaggag ctgtgagaga attaagataa
    30721 tatatgccac gttccttcgt aagcttcaga attggaatca tacaatttga atcgcgagtt
    30781 acagcagagc ccatatgatt gttcatgcct gcaggaggat atggaaagct atcgatattt
    30841 tcggctacag tcttttctat ggttgcttca tccattgaac aatagacagc atgagggcct
    30901 gggtcattgc ctcctaaagc ctccataggc tgatggagta ttagctcttt tccagcctgg
    30961 tgtattgccg ccacagaatc gttgctatgt ggaagctgag ggagaacggc tagagtaatc
    31021 ggaaagggaa gttggagaaa ggcatttagt tctccgttat tataaccaac atcgtcgatt
    31081 acaatgatga gcgtaggctg agctacactc cctggaaggg ctgatggtgg accagcagaa
    31141 ctataaggtt caagcttttc gcttccagct ttcttgattt cttcttcggc tccagcctgg
    31201 atgtctaaat ctgatgctgt ccttttggag atcgaagttt tgtctttcct ttcttcgggt
    31261 ggaatgaccg ctagatcttc gtatccagga ggtagctcaa ttacatattc tggtgttcca
    31321 taggggacat ccttggtcct gctgaacgtg tcttttgaat taatagaatt cactattatt
    31381 agggcggcga ttgccaaaaa agcagcgcta gaaagcaata ttgcatagta agagccctta
    31441 tgagtgcgct ttttcctcac gccatcatcg taccttggac agaatggttt atcaagcggt
    31501 taatccgact tgaagtgcag tatgccttag agattcaaga gccctctttt ctatatgtcg
    31561 taccgtttct gcggaacatc ctacaataga acctacagcc ttgagcgatt gttcgcgact
    31621 acagccgagg ccaaagcgtt tcgaaattac ttcttgctct cgctcgggaa gggtagataa
    31681 aagccaagag ctttgccatg tgcggctcga cttttctatt tcgagttcag ggttatatcg
    31741 ccagtcctcg tatacatcga gaattgagag ctcatcattg tttgtctgat caagagatac
    31801 aggctggtct cccatgttta gtattgcggc gatctcagtc tctttccagc ccgtctcgct
    31861 tgctaattcc ttttcggaag gatatctcga tagttcaagg ctcgcacgca tttgaatggc
    31921 tgatagacgc ctaagggcgt tttcttttct atgtggcaaa cgaatatgtc ggccggtatt
    31981 tactagcgaa cgcgatatag cttggcgaat ccaccagacg gcataagtag aaaatttaac
    32041 atttttcgat gggtcaaagc gctcagcggc ctttacaagc cctatattgc cttcttgtat
    32101 aagatccatg agcgaatgcg gcggagacca catagatttg gcaattcgta ccactagacg
    32161 aaggttccct tcgacaagcc tatctagggc ctgtttatct cctttagcta tttttccagc
    32221 taatactgct tcttcctgtc gggttaccaa gggaattttt cttatcgatt caaagtaaag
    32281 ctcgaggttt tcgtcgttgt cggcaatggt catgaatatt cctccaaaaa tgattcttct
    32341 gtaaaattag atgcaaatat cgtgccaaaa ttgcatcgaa agcagactat tctctatttt
    32401 tatctataat aaaggtatat tcgaatagaa atttgcaaga tgaaataaaa gggaaggtat
    32461 tgagggataa gtgtatgata tttaataaca atggcacagt ttatccgtct acaaaaagag
    32521 acattactgc tacgaacctt gtataaaaag ttgaagcata aagtagggaa gagggcgata
    32581 aagagaatgt acggaggtaa gcatgcattc ttttcctttc tgcccaaatc cagcctgtct
    32641 ctatcataag gctgct
//
LOCUS       DAOSOB010000015        32486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1403_length_32486_cov_68.397028, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000015 DAOSOB010000000
VERSION     DAOSOB010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 32486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32486
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1403_length_32486_cov_68.397028"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /gene="infC"
                     /locus_tag="PLS88_02830"
     CDS             <1..371
                     /gene="infC"
                     /locus_tag="PLS88_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOU06316.1"
                     /translation="EQEKKVKDAKKRAKVTELKEIRMQPKIAEHDLDFKANHVREFLE
                     DGNKVKVTIRFRGRELAHTEIGEEILQRILAKLEGSYVLERPPVMEGRFMSMVLQPKT
                     GAAKKQPEGDTITVVPGIEH"
     gene            391..591
                     /gene="rpmI"
                     /locus_tag="PLS88_02835"
     CDS             391..591
                     /gene="rpmI"
                     /locus_tag="PLS88_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOU06317.1"
                     /translation="MPKMKTKKAAAKRYKLTASGKVKYKKMNLRHILTKKTTKRKRNL
                     RKPGFLDAGPVYQIKKKLLPYG"
     gene            614..973
                     /gene="rplT"
                     /locus_tag="PLS88_02840"
     CDS             614..973
                     /gene="rplT"
                     /locus_tag="PLS88_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOU06318.1"
                     /translation="MPRAIDGTKRKDHRKKTLKIAKGYWGRRHSNHKAAKDAVAKALQ
                     DSYNDRRNKKADFRRLWIARINAACRMRDISYSRFMEGLSKADVKINRKMLADMAARD
                     PETFDTIVEKAKAALQG"
     gene            979..1758
                     /locus_tag="PLS88_02845"
     CDS             979..1758
                     /locus_tag="PLS88_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06319.1"
                     /translation="MLNLEQVRALEARVEKAVVLITKLRQENADLESQLADITRAEEL
                     LRNQNAELERQNAAQIRLVTEISEKLESLSTHVKEAEEKLAQAELKAAEAEERAATME
                     RKAQGAEEEIARYRDRALSAERRIAEMEAKAEELKSEQDKIEQGLIQALSKLDSFEDM
                     VLEMSIGSNPQGDRSLAQAEKIFSTSEGLSRKEASDKEVASEERGGLEASEPYLESKA
                     QEMSESSQGGGDLEEPADDSQPDSESQNAPFRGGQDELDIF"
     gene            1739..2092
                     /locus_tag="PLS88_02850"
     CDS             1739..2092
                     /locus_tag="PLS88_02850"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOU06320.1"
                     /translation="MSSISSSHPMHIEILGQSFTIKTDDSPEYIESLVEDLKQRYARI
                     SARMSVADPLRVAILAGLFLLDDLRKTESRPMSFKGKAPKDKASQPEGQLLSEDFDED
                     QIFEKLDKKLGELGL"
     gene            2185..2373
                     /locus_tag="PLS88_02855"
     CDS             2185..2373
                     /locus_tag="PLS88_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOU06321.1"
                     /translation="MPLESLINFSGNAYELAAAIAVRAYQLAVLRTPEVEKNNGKVVS
                     MSTQEILEKTVGYKLITS"
     gene            2348..3322
                     /gene="miaA"
                     /locus_tag="PLS88_02860"
     CDS             2348..3322
                     /gene="miaA"
                     /locus_tag="PLS88_02860"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOU06322.1"
                     /translation="MATNSLPVDAIILVGPTASGKTHLLDTIFGEGADSWIPRLRSLW
                     HADLYSAQVISADSMQAYRFMNIGTAKPSQDLLKRLPHHLIDIKNPNEQYTAGEFVER
                     ADTLCSLLKAQGILPIVSGGTGYYLMNFICGLPSSPPSSRAIRAQVAKDLEIFGAQAL
                     REELAKTDPKTAARIHERDIYRLTRAVEILRASGKPPSLFAPLKTIRSDRHFLLIGMT
                     RSREELHTRIHERVEAMMMEGLASENEKLKNLGYNSNDPGLRAIGYREFFELEGEPIE
                     RIANAIELHTRQYAKRQMTFFRALPGLIWIEPYPEDFFRALKEIGLRR"
     gene            3439..3921
                     /locus_tag="PLS88_02865"
     CDS             3439..3921
                     /locus_tag="PLS88_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOU06323.1"
                     /translation="MNADLIKTIVLSILPVSELRGAIPYAVYKGFSIPIAASISIAAN
                     ICVPAIAFLFLESLHRLLYKIPQYRRFFDSFVDRARTKVHTKVEKYGYWGLLLFVAIP
                     LPATGAWTGTLGAWVLGLSHKKAFFAIAGGVILAGIIVSILVALWGVSTQTIFFKPVS
                     "
     gene            4005..5258
                     /locus_tag="PLS88_02870"
     CDS             4005..5258
                     /locus_tag="PLS88_02870"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU06324.1"
                     /translation="MSEISLLNALLHGFDVVVRDPVWGDVHMDSSLHALFQSKAFMVL
                     DRIRQLGPVALVYPGATHTRKAHSLGVYHIARRMALALVEKGEIDFVSKTGLRSFLVA
                     ALCHDIGHYPYAHSLKELPLERHEALAGEMIISSLLRDYVVECGADPEQTASIIDPDR
                     TEDQSRETLLFRSFLSGVLDPDKIDYLTRDAFYCGVPYGIQDADYIMRRLIVHDDRLA
                     IDLKGEMSIEALIFAKYQMYRAVYWHPVVRAATAMVRKAVYLGLTSNALMPSQLYGID
                     DTSFNHLMNSSRFPPFRLAREAEEKRVYKLAAEHVFDESNPLHSDLLDIVKRTEAESA
                     LAQAAEIAENDVVIDIPERIKLETDLMVQVDNSFESFDQRSTLFCPKIVEKVAGALRT
                     IRIYIRPIDGIDSSKIASLVDTLLD"
     gene            5487..5930
                     /gene="mraZ"
                     /locus_tag="PLS88_02875"
     CDS             5487..5930
                     /gene="mraZ"
                     /locus_tag="PLS88_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOU06325.1"
                     /translation="MKSLTGEFKNTLDEKGRLSLPARLRANLSSTLVITRGIDPCLWL
                     FIPEEWEVLSQRILASTSLFQARSRLIQRRIIAPAQEVEVDKLGRIFIPQSLREWAGL
                     SKECVILAISRYIEIWDSQRYQAYMEEKQSEFLMAAEELGGLTLS"
     gene            5959..6963
                     /gene="rsmH"
                     /locus_tag="PLS88_02880"
     CDS             5959..6963
                     /gene="rsmH"
                     /locus_tag="PLS88_02880"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOU06326.1"
                     /translation="MQTQKSYSDKGNSTDYSLHIPVMLEQLCKLASTIRPMTLYIDCT
                     IGAGGHAEALLKEYEELRCIGIDADPDACNRASERLKPFADRLSIINGYYDEVLSEIR
                     NKRASYRFPGNVSSCLCNEKGELRPSLIFFDLGLSMYQLKSSGKGFSFYADEPLDMRF
                     SPSAQYTAEELVNQLPETKLANEIFKYGEERFSRQIAHAIVETRKKEYIYTSSQLASI
                     IYDAVHVKYRNGRIHPATRTFQALRILVNDELNRLERACIDALELLGPGAVCAVITFH
                     SLEDRIIKHIFAEKAKNSQFSLEWKKPITPTEEELRNNPSSRSAKFRAIRANDREAQI
                     "
     gene            6960..7265
                     /locus_tag="PLS88_02885"
     CDS             6960..7265
                     /locus_tag="PLS88_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06327.1"
                     /translation="MNARRFWIFSIIICLLFIGLVYQTTRYAALRTEVRELERSEMDL
                     LKVGRELDANIAKLANLEHIERAAIQKGMKVAAPEQRIIVTETHEKKASEVSNNGKQ"
     gene            7252..8721
                     /gene="murF"
                     /locus_tag="PLS88_02890"
     CDS             7252..8721
                     /gene="murF"
                     /locus_tag="PLS88_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOU06328.1"
                     /translation="MENNSLASDQQVLFYAGELAGIIGATCKGKLDTPIYSVVTDSRL
                     AEPNSMFVALKGERTDGHHHILEAIQNGARAILAEQEEKDIAIASIRNSGLVAEICLI
                     LADSPLSGLQYAAREYRRRHNLFRIGVTGSSGKTTTKECIASMLSACFPEESIAVSPG
                     NLNSDIGLALAIFGIKPQHKIGIFEMGINRKGEMDELAKMYEPDIAVITNIGSAHVGI
                     FGSRDEIAREKSKIFSYFNGQQKALLWEDDDYKVYLSSKVKGTVHYFGLRSTEGLGRI
                     ENKGLDGWRIEWKGLTLDFALPGKHNLLNACASLSMAILLDLDPILAVQGLSKVQPLF
                     GRSQLCKGKITVFQDYYNANPDSMAAAIDFLSGVSSTNRKVLILGSMLELGKESDKAH
                     RDVGFMVAGMNPEAVFLFGEEMKAAEAVLKEADFKGMLMYTTEMENLIDAVSNYIKEG
                     DLVLIKGSRGMALERLSRVFEEKGLINLSDSSKGGHYAP"
     gene            8711..9793
                     /gene="mraY"
                     /locus_tag="PLS88_02895"
     CDS             8711..9793
                     /gene="mraY"
                     /locus_tag="PLS88_02895"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOU06329.1"
                     /translation="MLREFLLPLVKYFTPFNIFRYLTFRSAYAAVTSLLISYVLGPWL
                     IDRLRVLKFGQSVRLDGPQSHLTKTGTPTMGGLLIILSVLISVVLWIDITSKYCWIAL
                     SALIGFGLIGAADDFLKIKKHSSDGLSAAQKLILQFIVSTIVVYAIYRTTGSSITKLY
                     VPFFKVHVVDLGVYWLPIAIIYVATWSNAVNITDGLDGLAVGLVIFAVLAFSVLTYVT
                     SRADWSQYLGLPYIKQASELTVFNFALLGACVGFLWFNAHPAEIFMGDVGSLSLGGVL
                     AVLSLMVKKEILLLVIGGVFVIEIGSVMLQVIYFKLTKGKRLFLMAPLHHHFELKGLK
                     ETKVVVRFWIVGGIFALIALSTLKIQ"
     gene            9803..10972
                     /locus_tag="PLS88_02900"
     CDS             9803..10972
                     /locus_tag="PLS88_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOU06330.1"
                     /translation="MKEESNIKGSLLDSHRIDWQMVSAIIIVSLIGIMTLWSGSIGFA
                     IRNGGPNSIIAKQAILYVVSLICMAIAAAIPIKLLRAVLPIAVVLCVIGLSLPLFSPL
                     GMTINKARRWIRIGSFSLQPSEIWKPVMVLYLASFLEKRKERLVESGLITFPAIILVI
                     VSAVLIFLQQDFSTSILVLAVAFAMLYLAGTPFIFMTNLALFLILYGALMIFSSQYRV
                     QRLVGFLMPDQHTQTVNYQMYSALRAIRAGGIWGKGVGLGTLKFSSIPEVQSDFIFAA
                     FVEEAGFAGVLMVMGLWFFILVRAGRRLIVSDYFSRLAGFGIITLLSLQVLLNLAVVT
                     GVMPTTGLALPFFSAGGSAILMSAISCGILINLSRSGQTETIMSAGFRQKGAAYD"
     gene            10965..12122
                     /locus_tag="PLS88_02905"
     CDS             10965..12122
                     /locus_tag="PLS88_02905"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOU06331.1"
                     /translation="MTDVRVQEQINETNAQRKKEARSLLSKFDNYSIDITKATTRSET
                     ALPGTSATKSPVKSIDTGDKIQISQRIRPSSEQDHPASKEKAGPVSTAKISIHPYIGH
                     ASQASVALSAQAEKLSEAVKPAKPSMAPRKNSGLRILIAVALLCILSAGAAISLPRLA
                     KIETVRVSGLETISENSIIESLGPIGNENLFSINIEQIANSITNNPRIASAKVHRVLP
                     STLAIDIRERLAVASIAINTEQGSKLILVDGEGIAFASLDADDSNNPELPVVSGIKFE
                     QFNLGQQLPEAILPLFANLAAIKQDSPELLKAFSEIHVERISANNIEIVLYPMHVKTA
                     VRLPLRFSAEALCNALVVLDILHSRGLSDQPSEIDFHSGTVIYQAKEAVSG"
     gene            12130..13350
                     /gene="ftsA"
                     /locus_tag="PLS88_02910"
     CDS             12130..13350
                     /gene="ftsA"
                     /locus_tag="PLS88_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOU06332.1"
                     /translation="MVVLDIGTTVTKAIVGGFNELGQFEIFGIGTAPSSGLRRGVVLN
                     IEGTLNSINSAIDAAELMSGIDIREVSLALSGANIESFNTNGQIAVTGRGREITAEDI
                     ARVHEAARAVSISMDREILHVVPRSYTVDDQKGIRNPLHMTGVRLECSVHIITGSVST
                     VQNMLKCVTRAGYKVEQSYLGILGCARTVLTEDERELGCLLLDIGGGTTDLMVFSEND
                     PVYTSAIPLGGSQITGDISIMLSIPIDTAERLKKESGAAWAEAVDPDETVIVPGFGAR
                     EPMELERRKLVSIVQPRAEEIFEMVNDRLIKNGLKELIKAGVVLTGGSALLPHIDDCA
                     RSVFGVPVRIGAPAALPGLDVGFRSPAFSAAVGIVLLEAERIGIPEPSTRKKARKGKE
                     GGFSLIKWIKDRFF"
     gene            13372..14619
                     /gene="ftsZ"
                     /locus_tag="PLS88_02915"
     CDS             13372..14619
                     /gene="ftsZ"
                     /locus_tag="PLS88_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOU06333.1"
                     /translation="MAIELVDDNGIGSPTVIKVIGTGGGGSNAVNRMIMAGLKNVQFI
                     AVNTDLQDLGRSKAEIRLGIGSKITKGLGAGGKPEIGEKAAIEDRDKIEQALRGADMV
                     FVTAGLGGGTGTGSAPIIAQAARDLGALTVAVVTKPFAFEGKIINRIAEEGFSKLKQA
                     ADTVIVIPNQNLLRVVDKKTPLKEAFRIADEVLRQGVQGISDLITLAGDINIDFADVR
                     TVMEGQGDAIMGIGIGSGDNRAQEASEKAINNPLLDDACIEGARNILVNVTCGEDFSL
                     IEYQEVIDLITSKAAEDAHIIVGVVTDSSLKDEVRVTVIATGFGPMRQKSGEKVGTKN
                     TIAMPLGSIRPSEFLSPGEWPLHEEKPAQNRDRGNENQDKPNFRDSFGKISFDPESDN
                     DEFDIPTALRNKRLIPEDFGGRR"
     gene            14616..15494
                     /locus_tag="PLS88_02920"
     CDS             14616..15494
                     /locus_tag="PLS88_02920"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOU06334.1"
                     /translation="MSILVRRFQAYLLSTRRRSILTADAYIREIEMLESFLFQYGKTL
                     LDASEEDLLTYLIRRSQSGLQRTTMARIIASLHSFYRFCLNEKLRADDPSIQIRTPKQ
                     DRNLPDVLDPDSIEHVLECIDISSPNGLRDRALFELIYSCGLRISEAADLKFQQLYLE
                     EKMLRVIGKRKKERLVPFGDEAKYWLTRYFTEARPFLEKHEKSDFIFLNQEGKGISRK
                     GIWKRFSQIREKSGVKAKVHTFRHSFATHLLAGGADLRTVQELLGHSDIATTQIYTHI
                     DEESLGMYHKEYFPRK"
     gene            15585..16559
                     /gene="dprA"
                     /locus_tag="PLS88_02925"
     CDS             15585..16559
                     /gene="dprA"
                     /locus_tag="PLS88_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOU06335.1"
                     /translation="MALEKKHEKKGRLMQLELDRISAIQPSMDNISLSLKLAFQRLHF
                     LSWQERILLLENIRDMEDFLALGLAEIESFLYRSMHARLLDMGRIWEQAERDVNILSR
                     LGARFVCIVDREYPPLLREIHRAPFGLFVRGDTKALIQPSVTIVGTRGPTSRGVLAAR
                     LLAKESADIGVVVVSGLARGIDAAAHRGAIQSGAGSTIAVLPCGPERVYPPSNAALAA
                     AILDCGGCLVTEYPPGVSLDRYRFPERNRILAGFSKLTLVVEAPEKSGALITAEFALG
                     EGRDVVVAASCLGSSRNAGADALNQDGALAINCAAELASMIQGECTGI"
     gene            16608..18767
                     /gene="topA"
                     /locus_tag="PLS88_02930"
     CDS             16608..18767
                     /gene="topA"
                     /locus_tag="PLS88_02930"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOU06336.1"
                     /translation="MAATRKKEKKTPGAAESADASKLKTLVIVESPAKARTIEKYLGS
                     RYKVLASNGQVIDLPKSRIAVDVAHNFEPEYITVRGKAEKLKELTEEAKRSRAVVLAS
                     DPDREGEAIAYQIGKYLEEKAKNTPISRVTFNEITRQAVQDAMANPRDIDMNLVEAQK
                     ARRVIDRLVGYTLSPLLWKKVKSGLSAGRVQSVALKLICDREKEVESFIPEEYWTIEA
                     HLKAHQHIVRAELALLNGDKPSIKSEAEANKILALFSGKSALVSDIQSIDRTIRPKPP
                     FTTSKLQQAASNRLGFTSTKTMKIAQQLYEGVDMGHQRTGLITYMRTDSTRISEAALH
                     ETHEWLTKYFPNQTPHTPIRYSVSNAAQDAHEAIRPTRVDITPDEAGRYLRGDQLKLY
                     ALIWEQFVASQMKPAVIRTLTADIQIGDGIFRNSASSFIEEGFYKVIRLAASKEERTS
                     HYLPFEKGEILLVEKIESEQHFTQGPSRYTDASIVRTLEELGIGRPSTYAPTIETLIE
                     RYYVQRDKRQLVPTQLGKIINDILSKNFPEVINTGFTAEMESMLDKVEEQKIDWVSEL
                     KKFYFPLVDKVENALNALEDMHGVLDEKTNEKCPICGRPLIKKLGRFGYFLSCSGFPE
                     CTFTKSVPLAICPKCGGDIVPRVSNKGRRKKFYGCSNYPECSFKTLYKPTNATCPKCG
                     WFLVEKYDKKTGHYKVCINPDCDYLHSSQQSGDNSGE"
     gene            18774..19670
                     /locus_tag="PLS88_02935"
     CDS             18774..19670
                     /locus_tag="PLS88_02935"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOU06337.1"
                     /translation="MDARFLDYLDYLGSVRGLSLRTVEAYRRDLAQYEVFLEGKDLDE
                     ADASDIRTFAGTLVMEKRAASSINRALSAVRGFYRYRMKFHEKGKDPAREVENVPTGR
                     SLPSFLFEEEINSFLESIEGETFLDVRDRALLEVMYSTGCRVSELCGMRLSRLNMNNF
                     TIRVKGKGAKERLVFLCDAANQALSRYLPYRAAVIRKYGIEEHDRIFVNSRGRPLSSR
                     GAEKIVEKRRLKAGIRKHLTPHTFRHSFATHLVAAGADMRVVQEMLGHSNISTTQVYA
                     HVDMERLRRVYEQAHPHGSKTK"
     gene            19688..20278
                     /gene="hslV"
                     /locus_tag="PLS88_02940"
     CDS             19688..20278
                     /gene="hslV"
                     /locus_tag="PLS88_02940"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOU06338.1"
                     /translation="MEIHATTILAVRKDGHVAMAGDGQVTMNNTVLKSNARKIRTIYN
                     GKVLCGFAGATADAFTLFEHFEAKVQEYGGDLTRASVELAKDWRTDRILRKLEAMLLV
                     ADQSKMFLLSGTGDVVEPSEDAIAIGSGGSYAYAAAIAYLDASRRIEEAAKSTASGDA
                     EMLHGSFSAREIAERSLKIASSICIYTNDQITVEEI"
     gene            20275..21642
                     /gene="hslU"
                     /locus_tag="PLS88_02945"
     CDS             20275..21642
                     /gene="hslU"
                     /locus_tag="PLS88_02945"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOU06339.1"
                     /translation="MITEKDALTPHKIVEELDRYIVGQEKAKKAVAIALRNRIRRQCL
                     PPEIREEIAPKNILMIGPTGVGKTEIARRLAKLCNAPFLKIEATKYTEVGYVGRDVES
                     MVRDLMAAAVSMVKEEMETQVENEAKRRAEERLLDILLPGLAKSDEPIGQSQATLIIP
                     SSGMETKEKFRSLLREGKLDSRQIEILVPAQGPQIELFAGTQFEEMNIALGGLQGLFG
                     GNKKKKTVSVAEALPILEAEEKEHLVDQEKIVQEARRRTQELGIIFVDEMDKIANREA
                     RGSSGIDVSREGVQRDLLPIVEGTIVNTKWGPIDTTHILFIGAGAFTVAKPQDLIPEL
                     QGRFPIRVELDALTGDDFVRILTEPKNALVTQYIQLLGTEDVQLEFTEDAIARIAEIA
                     AAANRQSENIGARRLQTVMERLLEDISFYADQHVGTKVIIDRAYVDERFKDYVQQQDL
                     SKYIL"
     gene            21659..22444
                     /gene="whiG"
                     /locus_tag="PLS88_02950"
                     /pseudo
     CDS             21659..22444
                     /gene="whiG"
                     /locus_tag="PLS88_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525971.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
     gene            complement(22470..23882)
                     /locus_tag="PLS88_02955"
     CDS             complement(22470..23882)
                     /locus_tag="PLS88_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU06340.1"
                     /translation="MRATLLVVDDETNIREGLAQLFSMEGYDVRTAKDGIEGKMIVDK
                     EDIDVVITDYKMGRMSGLDLLHYIVSTHPGIPVIILTAHGSVDKAVEAMREGAFDFIA
                     KPPNTDHLINLVRRALETRELYRTNIELKAEVESQRARSYIIGKAPSIKHIFDLIRKV
                     APTKASVLITGESGVGKELVADAIHNLSPRKDKPFIKVHCAALAESLLESELFGHEKG
                     AFTGATGRKRGRFEMADGGTLFLDEIGEINQNVQIKILRVLQERKFERVGGEETIEVD
                     VRLIAATNRDLKKEIAEGRFREDLYYRLNVVNIHVPPLRERREDIPLLAMAFLREFAD
                     ENGKQIEGFDPKVRQALYTYDWPGNVRELRNCIESAVVMASGKLITLDDLPPGPRSSG
                     EARTISIPAFSSLEEAERIIIAETLALVGGNKSKAAEILKIGRKTLYQKIEQYGIGKH
                     EASSSSSSSSREKTEIQQNS"
     gene            complement(23883..25076)
                     /locus_tag="PLS88_02960"
     CDS             complement(23883..25076)
                     /locus_tag="PLS88_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU06341.1"
                     /translation="MKAIYENTLKRIDRIPPEGLKQIIKNLLAENSIYESALNSTLDG
                     IIVCDLAHKPLFINRSAEKITRIVSWTPETVLWEQLNDDNLADFIQEALKSEKNILNR
                     ELSLGRPGGTRLLSISISTLVSEGKIAGNLIHIEDITEKRRREAQLRRAESLAALTTL
                     AAGVAHEIKNPLASIRIQLGIIRRILEKKYNKKTEPIFNNISLVEQEIDRLNSIVVDF
                     LFAVRPMDITLIRDSIEDVIKEVVEIMGNEASEQHINIVTKFEDNLPEVMIDRKHLKQ
                     ALLNIVKNAIAAMPDGGTLTISAVTKDDELQISISDTGIGIPEELLAKIFEPYFTTKE
                     SGTGLGLTITFKIIKEHNGEITVESKPSKGSTFTIHLPIPQPEQKSLPPWEDNIQIEG
                     SEREE"
     gene            complement(25060..26358)
                     /locus_tag="PLS88_02965"
     CDS             complement(25060..26358)
                     /locus_tag="PLS88_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06342.1"
                     /translation="MFENLLFQNHALEQLVSLAKEKKVPPALLFVGPAGSGKLTAALE
                     CARVLSCEKAGEWNCQCIQCNCHRNLMHPDLLLFGPREFPQETIVTGEYLIRKPNLTS
                     YYAFARSIRKLLKRFDSVLWQGEEARLSKAIQSIETCEEMLQEILEQVLKKKNHGLSA
                     LVDSAVNAAAGLESLVPDGIPIFMIRNMISWAVLAPSAKTKMIIIQNADAANESSRNA
                     MLKILEEPPETARFILTSSSRATIIATILSRSRLISFDPRTDYQAREIISRLFHANED
                     IQEINSISEFFQNKSAYPPKIAIQDAALFVGALLGKAMEKDAGLSGERALELASMAQG
                     QNVSTLSMLRQVSEKTNGFGAKNSKFTNSFLVFIRGVAQHLTSIATDPSSSASLTMYV
                     DRLFSKLREMNMQYRTLNRSPELLLEAFADIFGEQYESHI"
     gene            complement(26361..26894)
                     /locus_tag="PLS88_02970"
     CDS             complement(26361..26894)
                     /locus_tag="PLS88_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOU06343.1"
                     /translation="MSTIDWIFSGVVIILAARCFVRGFIHEILSVASIAVGILVGLLL
                     SNTVILQILPKIKADKLPYQVQYIIAFILCFIAGFLLMKIIERMLREGLEASRLDILD
                     RILGLVLGVAEGFIVVGLFIVILEIQPIVDVKAILSNSLYVRLLGPIIEPAIGVTLTP
                     MIKSGEIPSLKQNPRGK"
     gene            complement(26891..28135)
                     /locus_tag="PLS88_02975"
     CDS             complement(26891..28135)
                     /locus_tag="PLS88_02975"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOU06344.1"
                     /translation="MGEGKMQSIHKTEPQMKDEHEKACIAFTGGGTGGHIYPGLAVIE
                     ELRKLGFKGRIVWIGSEKELDRSIVIGQGIEYYAIPSGKFRRKLSLRNISDLGRIASA
                     YFRARDILKDIHPSILFSKGGYVSVPPCRAAASLGIPVITHESDASPGLATRLNSKKA
                     SLILTSWEVTAQYFPESLRSKIIKTGNPVRPDILNGDRQRGLSMLEFVAEKPVLFVLG
                     GSQGAQEVNDIVLSSLSLLVPLVQIVHQTGRANFDEVKAKIPDDPDVRKAYRPLPYIG
                     SEIADIYAASTIIAGRAGAGAIWEAASLGKPMILIPLSGSGTRGDQVENAAIAARSGI
                     ATVLQGPNIPSDLFADAVKKYLDFENYAMAEEACHSFVRIANTPYIAEVSDTLSQNPM
                     FSAEYIARLLLKKIAEGAKDSA"
     gene            complement(28159..29766)
                     /locus_tag="PLS88_02980"
     CDS             complement(28159..29766)
                     /locus_tag="PLS88_02980"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOU06345.1"
                     /translation="MVLFKNLDTCQAFEVLKKMPAIELRESLKKERISDWWIPHAAGF
                     YYCYAFAPVDDAIIDELEALAREQQLIEKYRMLTEGAIINTSEKRMVLHHLARGQLGK
                     PVILNGQDLEVFYREEKHRFYDFAKKIREGKILSSADKAFTEIVQIGIGGSDLGPRAA
                     SLAISRWAAAKGRARLRPWFISNVDPDDADYVLANIDLSKSLFVLVSKSGTTQETLAN
                     ETLVRDRLQRAGLNPDKHIVAVTSTSSPLAHNPHYLDSFYIDDFIGGRYSTSSAVGGV
                     IISTAYGPETFEEFLEGANEADRLAFEPSFRKNPMLMDALIGIYERNILGWPATAVLP
                     YTESLSRFPAHLQQLDMESNGKSVNREGHPISYSTGPLVFGEPGTNGQHSFYQFLHQG
                     TDTIPLQFIGFLRNQLSQDMISEGSSSRQKLLANLIAQIHAFAVGKKDENLNKAFPGN
                     RPSTLLLAQELGPRQLGALYSHFENKVMFQGFVWNLNSFDQEGVQLGKVLAKKALNAI
                     SKGESTDDELLDAFIVSALSAVKNHGD"
     gene            complement(29849..31528)
                     /locus_tag="PLS88_02985"
     CDS             complement(29849..31528)
                     /locus_tag="PLS88_02985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOU06346.1"
                     /translation="MLKLDLASEPEDPIGVLGRERFHIPHLTPLQRYVIANILDEIKG
                     STELDGDPSLNLIVDDPIVNQDEKAEITERQETYRQLILFPTGFGKSVCFQLPSLLLD
                     GLTIVVYPLLALMNDQKRRLDEANIPCALFRGGLSKEEWHEQETLITSGKATMVVVNP
                     EILSAPKLRRFLKQFRIAHFVVDEAHCISEWGESFRPSYLALGEAVEAISPRILSAFT
                     ATAGPHIVQSIEKKLFMDKNYQLVDAEADRPNILYAVVHTLSITRTLRQTLLCCKKPA
                     IIFDRSRPGTRLKAELLKASGFYQTRFYHAGLDRVERCSIEQWFQNSDDAILVSTNAY
                     GMGMDKKNIRTVIHSSLPESAEAYIQEAGRGGRDGKNALAILIDDLAAPYSQYHKANK
                     KDDKEKDIQSIRKACFLPYPTLRSCRREFLLHLLGEQQTPECGKCDNCIEGSHTLKMQ
                     LGLDASDIPPEWEFLSKTEGFIETISFCASHQGVFTPQELIDALGAYGKGKGKYAGCL
                     YGWLKIEREELINSLSSLDILYVPEKGTWKNRILLSKKGKTLSRLAQNSLG"
     gene            complement(31558..32268)
                     /gene="nth"
                     /locus_tag="PLS88_02990"
     CDS             complement(31558..32268)
                     /gene="nth"
                     /locus_tag="PLS88_02990"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOU06347.1"
                     /translation="MSSELGITLKDKNESMTLPQLLKELYIRLVPIWPDTRPLLHYRS
                     CFELLIAVILSAQTTDDQVNIVTKELFRRFPNAESLADANIHEVENIIHSLGFYRVKA
                     KHIIETAKVLKERFNGQIPSSFEEMIELPGVGRKSANLLISACYEKPGIIVDTHVIRV
                     LFRLGVSTKKEATLIESIIRANLPPEKHTKFSHAVNRHGKFTCTARKPVCTLQEVYCP
                     VVDLCPKIGLSNLLKQRQ"
BASE COUNT         9215 a         6766 c         7595 g         8910 t
ORIGIN      
        1 ttgagcaaga aaaaaaggta aaagatgcaa aaaagcgagc aaaggtcacg gaactcaagg
       61 aaattcgaat gcagcccaag attgctgagc atgatctcga ctttaaggcg aatcatgtgc
      121 gagaattcct agaggatggc aataaggtca aagtgaccat tcgcttccgt ggacgagagc
      181 tggcgcatac ggaaataggt gaggaaatcc ttcaaagaat tcttgctaaa ctggaaggct
      241 cctatgtact cgaacgacct cccgttatgg aaggtcgatt tatgtcgatg gtgcttcaac
      301 caaagacagg cgccgcaaaa aagcagccgg aaggcgatac tattactgtc gtcccgggaa
      361 tagaacattg atttagaagg atggtacata atgcccaaga tgaagaccaa aaaagccgca
      421 gcaaaacgtt ataaactcac agctagcggc aaagtaaaat acaagaaaat gaacttgcgc
      481 cacattctca ccaagaagac tacaaagcgt aagagaaact tgaggaagcc aggattcctt
      541 gacgcaggtc ccgtatatca gatcaagaaa aagcttctcc cttacggctg acagaattgg
      601 aggatgaaag gctatgccga gagcaataga tggaacaaaa cgaaaagatc atcgcaaaaa
      661 aacactgaag attgcaaaag gttactgggg tcggcgacat tccaatcata aggcagcaaa
      721 ggatgcggtc gccaaagcgc tccaagattc ttacaacgac aggcgcaata aaaaagcaga
      781 tttcagaaga ctctggatcg ctcgcataaa cgcagcttgt cgcatgaggg atatcagtta
      841 tagtcgtttt atggaagggc tttcgaaagc tgatgtaaaa ataaatcgaa agatgcttgc
      901 tgatatggct gcgcgtgacc cggaaacttt cgatacaatt gtggagaagg ctaaagcagc
      961 ccttcagggg taaagtatat gttgaatctt gagcaggttc gtgcgctaga agcgcgggtt
     1021 gaaaaagcgg ttgtccttat tacaaaactg agacaagaaa acgcggactt ggagagtcag
     1081 cttgcagaca taactcgtgc agaggagctg ctcaggaatc agaatgctga actcgaacgt
     1141 cagaatgcgg ctcaaatacg attggtcacg gagatatccg agaagcttga gagccttagc
     1201 actcatgtca aagaagccga agaaaagtta gcccaagccg aactaaaggc tgccgaagcc
     1261 gaggagcgtg ctgccactat ggagcgcaaa gctcaggggg ctgaagaaga aatcgcacga
     1321 tatcgtgatc gggcactttc tgcagagcga cgaattgccg agatggaagc taaagcagaa
     1381 gaactgaagt ctgagcaaga taagatcgag caggggctga ttcaagcact ctcaaagctt
     1441 gattccttcg aagacatggt gctcgagatg tcgataggat caaacccaca gggagacaga
     1501 tctcttgctc aagcggaaaa gattttttct acatctgaag ggttatcaag gaaagaagct
     1561 tcggataaag aagtagcttc ggaggaaaga ggcggtttgg aagcatccga gccatatctt
     1621 gagagcaaag cacaggaaat gtctgaatcg agtcaaggag ggggagattt agaggagccg
     1681 gcggacgatt cccagccgga ttctgagtcc cagaatgccc cctttcgagg aggccaagat
     1741 gagctcgata tcttctagcc atcccatgca tattgaaata cttggtcaat cttttacaat
     1801 caagaccgat gatagtcccg aatatatcga gagcctggta gaagatttga aacagaggta
     1861 tgcaagaatc tctgccagga tgagtgtagc tgaccctctg cgtgttgcga ttttggcggg
     1921 tcttttcctt ttggatgatt taagaaaaac agaaagccgt ccaatgtcgt tcaagggtaa
     1981 agcgcctaag gataaggcca gccaaccgga aggccaactg ttgagtgaag acttcgatga
     2041 agaccaaatt tttgaaaagc tagataagaa gctcggagaa cttgggcttt gattaccgat
     2101 tattggctat attcttatat tttgatatat tctgttttaa ttatttgaaa tcaagtccta
     2161 gacagaggag agtttttgat gataatgccg cttgagtctc ttataaattt ctcaggaaat
     2221 gcgtacgaat tggctgcagc aattgctgtt cgagcatatc agcttgccgt attgcggaca
     2281 ccagaagtag agaaaaacaa cggcaaggtc gtttctatgt cgactcagga aatcctcgaa
     2341 aagaccgttg gctacaaact cattaccagt tgatgccata atacttgttg gtccaactgc
     2401 atctgggaaa acccatcttt tagacactat ttttggtgaa ggcgcagatt catggattcc
     2461 gcgacttcga agcctgtggc acgccgatct ttattctgct caagtaatct ctgcagattc
     2521 tatgcaggct tatcgattta tgaatattgg aacagctaag ccgtcacagg atttgctaaa
     2581 gagacttcct catcatctta tagacataaa aaatcctaac gagcagtata cagcaggcga
     2641 gtttgtcgag cgagccgaca cattgtgctc tctgcttaaa gctcaaggga ttctgcctat
     2701 cgtgagtggt ggaactggct attatctcat gaattttatc tgtggcttgc cttcttctcc
     2761 gccgagttct cgagcaattc gtgcgcaagt cgccaaagat ttagaaatat ttggggctca
     2821 ggccttgaga gaagaacttg caaagaccga tcccaaaact gctgctcgaa tccatgagcg
     2881 agatatctat agacttacga gagctgtcga gattctcaga gcctcaggaa agccaccaag
     2941 tctattcgct cctctaaaga ctattcgttc agacagacat tttcttctga ttgggatgac
     3001 gcggagccgg gaggaattgc atacacgaat ccatgaacga gttgaagcta tgatgatgga
     3061 aggtcttgca tctgaaaatg agaagcttaa gaatcttgga tataattcca acgatccagg
     3121 tcttcgagcc attggatatc gggaattctt cgaacttgaa ggggagccaa ttgaaagaat
     3181 agcaaatgcc atagaattgc acacaagaca atatgcaaaa cgtcaaatga cgtttttccg
     3241 tgctttacca ggacttattt ggatagagcc atatcctgaa gattttttcc gtgctctcaa
     3301 agaaatcgga ttgcgacgct aaggcctgtc tattcacagc aatgttctgt gtctcttaat
     3361 attgtaagat aatgccagtt tctatagggc tgaatctttc tctagacagg cttaggcgag
     3421 ctcgttatag tgcccctgat gaatgcagac ctcattaaga cgattgttct ttctattctt
     3481 cctgtatcgg aactgagggg agcaattcca tacgctgttt acaaagggtt ttccattcct
     3541 attgctgctt ctatatcgat tgcggcgaat atctgcgtgc ctgctatagc ttttttattc
     3601 ttggagagtc tccataggct tttatacaag attccgcaat ataggcgatt ctttgacagt
     3661 tttgtggata gagcgcggac aaaagtgcat acaaaagtgg aaaaatatgg ttattggggt
     3721 cttttgctct ttgtggctat acctctgcca gccacgggtg catggacagg gactctaggt
     3781 gcttgggtac ttggcctttc gcataaaaaa gccttttttg ctattgctgg aggtgtcatt
     3841 ctagcaggca taattgtcag catcttggta gccttatggg gagtcagcac tcagactatt
     3901 ttcttcaaac cagttagctg agttgcatga tccgagaggt ggctggtaga atttaatcga
     3961 ggaatgatag acatatatac ccttttaaaa aggctggttc aggcatgtct gaaatttcgc
     4021 tcttgaatgc acttttgcat ggcttcgatg ttgtagtaag ggatcctgta tggggagatg
     4081 tgcacatgga ttcttcgctc catgcgctct ttcaatccaa agcctttatg gtgctagatc
     4141 gaattcgaca actgggtcct gtggcgctag tgtatcctgg cgcaacacat acgaggaaag
     4201 cccatagcct tggggtctat catatagccc gacgtatggc gctagcttta gtggaaaagg
     4261 gagagataga ctttgtcagc aagacagggc ttcgctcttt tttagttgca gccctttgcc
     4321 atgatattgg ccattatcca tatgctcatt ctttaaaaga acttcccctt gagcggcatg
     4381 aagcgcttgc tggagaaatg atcatttcta gtcttctacg agattatgtt gtggagtgtg
     4441 gcgctgatcc tgaacagact gcatctatca tagatcccga tagaacagag gatcaaagca
     4501 gagagacgct tctttttcgc tcttttttat caggtgtact cgaccccgat aagatcgatt
     4561 atttgacgcg ggatgcattc tattgtggtg ttccatatgg catacaagat gctgactata
     4621 taatgagacg actcatagtg cacgacgata ggcttgccat agatttgaaa ggcgaaatga
     4681 gcatcgaggc tcttatcttt gcaaagtatc aaatgtatcg cgctgtttac tggcatcctg
     4741 ttgtaagggc tgcaacagcc atggtaagaa aggcagtata cctaggattg acttctaatg
     4801 cacttatgcc ttcgcaactg tatggtatcg atgataccag tttcaatcac cttatgaata
     4861 gctctagatt tcctcctttt aggcttgcaa gagaagctga agagaaacgt gtatacaagc
     4921 ttgcggctga gcatgtattc gatgaatcca atcctctaca cagcgatcta ttggatatcg
     4981 taaagcgcac agaagcagag agtgcgcttg cccaagcggc agagatagca gaaaacgatg
     5041 tagttatcga tattcctgag cgaatcaagc tagaaactga tctgatggtt caagtggaca
     5101 attctttcga atctttcgat cagcgttcga ctctattttg tccaaaaata gtcgaaaagg
     5161 tagctggagc gctgaggaca attagaatct atattcgccc catagatggc attgattcta
     5221 gtaagattgc atctctggtc gatactttgc ttgactgaga atctatatac agatcaaatg
     5281 caagctttct atttcaaaag actctaactc cttaccataa ataatattct cttgaatctc
     5341 atgatgctat acatatgtat agcgaaaaag gatgcgattt ttgttttctg tcgattcttt
     5401 ttctaaagtt ttgttgacac atgcagatta gcgcttaaaa tgataaatga gtggtaaatt
     5461 gtggtaaata gtaggaaatg gtggaaatga aatccttgac tggagaattc aagaatacgc
     5521 tggatgagaa aggccgtctt tctcttccag cccgtttgcg tgctaatcta tcgtcgactc
     5581 tagtaatcac aagagggata gatccatgct tatggctttt tatacctgaa gaatgggaag
     5641 ttctttctca gcgaattctt gccagtacaa gcctctttca agcacgatct cgcctaatac
     5701 agagaagaat aattgctcca gcccaagaag tggaagtgga taaactgggt agaatcttta
     5761 ttcctcagag cctgagagaa tgggcaggac tatcgaaaga gtgcgttatc cttgcgattt
     5821 ctcgctatat cgaaatatgg gacagccaaa gatatcaagc ttatatggaa gaaaaacaat
     5881 cggaattctt gatggctgcc gaagagcttg gcgggcttac gctttcttga gggattgtct
     5941 atgcaaggca agcggattat gcaaacgcaa aaatcctatt ccgataaagg gaacagcacg
     6001 gattattccc tgcatatacc agtaatgctg gaacagctat gcaagcttgc ctctacgata
     6061 aggccgatga cactctatat agattgcaca attggggcgg gtggccatgc tgaagcgctt
     6121 ttgaaagaat atgaggagct tcgctgcatt ggcatcgatg cagatccaga tgcctgcaat
     6181 cgcgcatctg agcgtcttaa gccatttgca gataggcttt cgatcattaa cggttactac
     6241 gatgaggtgc tatctgaaat tcgcaataag agagcaagtt atagatttcc tggcaacgtt
     6301 tcttcgtgtc tttgcaatga aaaaggcgag ttaaggcctt ctctcatttt tttcgatctt
     6361 ggtctttcga tgtatcaact caagagttca gggaaaggtt tttctttcta tgcagatgag
     6421 cccctggata tgcgtttttc tccatctgcg cagtatactg ccgaagagct tgtcaaccag
     6481 cttccagaaa caaaactggc aaatgaaatt tttaaatatg gtgaggaacg attttcaaga
     6541 caaattgcgc atgcaatcgt agagacaaga aaaaaagaat atatctatac atcatctcag
     6601 ctggcttcga ttatatatga tgcagtacat gtcaaatatc gcaatggtcg cattcatcct
     6661 gcaactcgca catttcaagc gcttcgtatc ttggtcaacg atgagcttaa tcgcctcgag
     6721 agagcttgta tagatgccct ggaattgctc ggccctggtg ccgtctgcgc agtgataact
     6781 tttcattcgc ttgaagatcg cattatcaag catatcttcg ctgaaaaagc aaagaatagc
     6841 caattttcgc tcgaatggaa aaagccaata actcctacgg aagaagagct ccgcaataat
     6901 ccctcctcac gatctgctaa attcagagcc attcgagcaa atgatcgaga ggcacagata
     6961 tgaatgcacg gcgtttctgg atttttagca taattatctg cctcttattc ataggactag
     7021 tgtaccaaac aacgcgctac gctgctctga gaactgaagt aagagaatta gagcgctctg
     7081 agatggatct cctaaaagtt ggcagagaat tagatgcaaa tattgcaaag ctcgctaacc
     7141 tagagcatat cgaaagagca gcgattcaaa aagggatgaa agttgcagct ccagaacaac
     7201 gtattattgt aactgagaca catgaaaaga aggcttcgga ggtgagcaat aatggaaaac
     7261 aatagccttg cttccgatca acaggtcttg ttctacgctg gcgaacttgc aggtatcatt
     7321 ggagctactt gtaaaggcaa gctcgatacg ccgatatact ccgttgtcac tgattctagg
     7381 ctagcagagc caaatagcat gtttgtagct ctcaagggtg agagaacaga cggccatcac
     7441 catattctcg aggcaattca aaatggagca agggcaatcc ttgccgagca agaagaaaaa
     7501 gatatagcta ttgcttctat tcgaaatagc ggtctagttg cggaaatctg tcttattctt
     7561 gctgattctc ctctttcagg ccttcagtat gcagctcgcg aatatcgaag aaggcataat
     7621 ctattcagaa taggagtgac tggatctagt gggaagacga ctaccaagga atgcattgct
     7681 tccatgcttt cggcttgttt cccagaagaa agtatcgcgg tgagccctgg gaatctgaat
     7741 tccgatattg ggcttgctct ggccattttt ggtattaagc ctcagcacaa gataggaatc
     7801 tttgagatgg gcataaatcg caagggtgag atggatgagc tcgctaaaat gtacgaacct
     7861 gatatagctg tcataaccaa tatagggtca gcgcatgtag gtatatttgg ctcgagagat
     7921 gaaattgcga gagaaaaaag caagatattt tcatatttca atggacaaca gaaggctttg
     7981 ctatgggaag atgatgatta caaggtctat ctctcttcaa aagtgaaagg cacagtacat
     8041 tattttgggc ttcgcagcac cgagggtctt gggcgaatcg aaaacaaagg cttagatggc
     8101 tggagaatcg aatggaaagg cctaacctta gattttgccc ttcctggcaa acacaatctt
     8161 ctgaatgcat gtgcttcctt atcaatggcg atacttctcg accttgatcc tattcttgcg
     8221 gttcaagggc tttcgaaagt tcagcctctc tttggccgct cacaactttg taaaggcaaa
     8281 atcactgtat ttcaggatta ttacaatgca aatcccgata gcatggccgc cgcaatcgac
     8341 ttcttgtctg gagtctcatc cacgaatcgc aaagtcttga ttcttggctc tatgttggag
     8401 cttggaaaag aaagcgataa agcacatcgc gatgttggtt ttatggtggc gggaatgaat
     8461 ccagaagctg tttttctttt tggagaagaa atgaaagcgg ccgaagctgt gctaaaagaa
     8521 gctgatttta aaggtatgtt gatgtatacg acagaaatgg aaaatctgat cgatgcagtt
     8581 tccaattaca ttaaagaagg tgatcttgtt cttataaagg gatcacgagg tatggccctt
     8641 gaaaggctct cgcgtgtatt tgaagaaaaa ggactcataa acttgtctga ttcgagtaag
     8701 ggaggacatt atgctccgtg agtttcttct tcctttagta aagtatttta ctcccttcaa
     8761 tatttttaga tatttgactt ttagatcggc ttacgctgca gttacgtctc ttctgattag
     8821 ctatgtcttg ggtccatggc ttatcgatcg attaagggtg ctcaagtttg gtcagtctgt
     8881 gcgattggat ggccctcaga gtcacttgac gaaaacagga acacccacta tgggcggtct
     8941 tttgataata ttgtcagtct taatatctgt tgtgttatgg attgatataa caagcaaata
     9001 ttgttggatt gcactttcgg cgcttatagg ttttggcctc ataggagctg cggatgactt
     9061 ccttaaaata aagaaacata gttcggacgg gctctctgct gcacaaaagc taatcctaca
     9121 atttattgtc tcgaccatag tcgtctatgc gatttaccgc acgactggtt cctctatcac
     9181 aaagctctat gttccatttt tcaaagtcca tgtcgtagat ctcggagtct attggttgcc
     9241 tatagcaatt atttatgttg caacttggtc gaatgcggtc aatataaccg atgggcttga
     9301 tggcctcgca gtaggtcttg ttatctttgc agttctagct ttttctgtcc ttacttatgt
     9361 aacaagccga gcagattggt cgcagtatct agggcttcca tacataaagc aggcaagcga
     9421 gctcacagtt ttcaattttg ctttgttggg tgcctgtgtg ggattcctct ggttcaatgc
     9481 tcatccagcc gagatattta tgggggatgt cggttctcta tcccttggtg gagtccttgc
     9541 agtgctatcg ctcatggtaa aaaaagagat ccttcttttg gtcattgggg gtgttttcgt
     9601 aattgaaatt ggctcagtaa tgctgcaagt gatttacttc aagctgacta aagggaaaag
     9661 gctttttcta atggcgcctc tccaccatca cttcgagcta aagggcttaa aggaaacaaa
     9721 agttgtggtg aggttttgga tagtgggggg catttttgcc ttgattgcat tgtcgacttt
     9781 gaagattcaa tgaggttggt tgatgaaaga agagagtaat ataaagggct cgcttttgga
     9841 ttcgcatcga atagattggc aaatggtcag tgcaataatc attgtctcgt tgataggaat
     9901 aatgacatta tggtctggct ccattggttt tgctatacgt aatggtgggc ctaactcgat
     9961 aattgccaaa caagcgattc tttatgtggt atctcttatt tgcatggcca tagccgcggc
    10021 aattccaata aagctattgc gagcggtttt acctattgca gtcgtcttat gcgtaattgg
    10081 tctctctttg ccgctattta gtcctctagg aatgacaatc aataaggcgc gccgttggat
    10141 cagaatcggt tcgtttagtc ttcagccttc agaaatctgg aaacctgtaa tggtgctata
    10201 tctcgcatct tttctcgaaa aacgaaaaga gcgactcgtc gaatcaggcc tgataacttt
    10261 ccctgcaatt atccttgtta tcgtatctgc ggttctgatt tttctacagc aggatttttc
    10321 gacttcaatt ctagtacttg ctgttgcatt tgcgatgctg tatctcgcag gtactccatt
    10381 catattcatg actaatctgg cattgttctt gattctctat ggtgcgctta tgattttttc
    10441 ctcacagtat agagtgcagc gcttagttgg ttttctcatg ccggaccagc atacccaaac
    10501 agtgaattat cagatgtata gcgcactcag ggccattcgt gccggtggta tctgggggaa
    10561 aggagtaggg cttggcactc taaaattcag ctctattcca gaagttcaat ctgattttat
    10621 atttgcagct tttgtagaag aagctggatt tgctggtgtc ttaatggtta tggggctttg
    10681 gttcttcata ctggtgcgag ctggcagaag acttattgta tccgattact tttcaaggct
    10741 tgcaggtttt ggaatcataa cccttctatc cttgcaggtg cttttaaatc tcgctgttgt
    10801 aacaggagta atgccaacta caggtctagc tcttcctttc ttttcggctg gtggctcggc
    10861 aattctcatg agcgcaattt cttgtggtat tctcatcaat ctatctcgaa gtggtcagac
    10921 tgagacaatt atgagcgcag gctttaggca aaaaggggca gcatatgact gatgttagag
    10981 tgcaagaaca gatcaatgag acaaacgccc aaagaaagaa agaagcgcgt tcgcttctat
    11041 caaaatttga caattattcc atagacatca ccaaggctac tacacgctct gagacggctc
    11101 tgccaggcac ttccgcaacg aaaagcccag taaaaagcat agatacgggc gataaaattc
    11161 aaatatcgca gagaattaga ccctcctcgg aacaagatca tccagcgtca aaagagaaag
    11221 ccggacctgt atctaccgca aagatttcta tacatccata tataggccat gcttcacaag
    11281 cttctgttgc gctttccgca caggccgaaa aactcagcga ggctgttaag ccagccaaac
    11341 catcgatggc tcctaggaaa aatagcggct tgcgaattct catagcagtt gcgctattgt
    11401 gtattctttc ggcaggagca gctatctcac tcccgcggct tgccaagatc gagacagtta
    11461 gggtaagcgg cttggaaact atatcggaga attccataat tgagtcctta ggtccgattg
    11521 gaaacgaaaa tcttttttcg ataaatatag aacagatcgc aaattcgatt acaaataatc
    11581 caagaatagc tagcgcgaaa gttcatcgag ttttaccttc tacactcgct atagatattc
    11641 gtgagcgttt agcagtggca tcgatagcta ttaacacaga acaaggctca aaactcattt
    11701 tggtcgatgg cgagggcata gcatttgctt ctctagatgc agacgattct aataatcccg
    11761 agctgccagt agtatcgggc attaaatttg agcaattcaa cttaggtcag cagctacctg
    11821 aagccattct accgcttttt gcaaatctag cggcgatcaa gcaagattcg cctgagctac
    11881 tcaaggcgtt ttcagaaata catgtggaga gaatctcggc aaacaatatc gagattgtgc
    11941 tttatcccat gcatgtaaag acagcggtac gcttaccgct cagattcagt gccgaagctt
    12001 tatgcaatgc attggtggtt cttgatattt tacattcgcg tggcttgagt gatcagccaa
    12061 gcgaaataga ttttcattcc ggcactgtaa tctatcaagc aaaggaggca gtctctggct
    12121 gatacaagta ttgtagtcct cgacatagga accactgtta caaaagcgat tgttggcggg
    12181 ttcaatgagt tgggacaatt tgaaatcttt ggcataggca cagcgccttc gagtgggctg
    12241 cgacgcggag tagtactcaa tatcgaaggc actttgaata gcatcaactc tgcaattgat
    12301 gcagcagaac ttatgtcggg aatagatatt agagaagttt cgctcgctct ttcaggtgcg
    12361 aatatcgaga gtttcaatac taatggacag atagcagtaa cagggcgagg ccgagaaatt
    12421 acagcagagg acatagctcg cgttcatgag gctgctcgtg cggtatcaat ttcaatggat
    12481 cgagaaattc ttcatgttgt gcctcgatcc tatacggtgg acgatcaaaa gggaattcgc
    12541 aatccactgc acatgacggg agttcggctt gaatgcagtg tccatatcat aacaggctcg
    12601 gtatcaacag tacaaaacat gttgaaatgc gtaactaggg caggttacaa ggtcgaacag
    12661 agctatctgg gaatattggg ctgtgcacgc acggtgctaa cagaagatga acgcgaatta
    12721 ggttgtcttc tcttggatat aggaggaggt actacagact tgatggtttt ttccgaaaat
    12781 gatcccgtct atacctctgc tattccctta ggaggaagcc aaatcacggg cgatatatcg
    12841 ataatgctga gtattcctat cgatacagcc gagcgtctaa aaaaggaatc tggagccgca
    12901 tgggccgagg ctgtagatcc tgatgagact gtcattgtgc caggatttgg agcacgcgag
    12961 ccaatggaac tcgaacgtag aaaactggta tcgattgtgc aaccacgcgc agaagagata
    13021 tttgaaatgg taaatgatcg attaataaag aatggattaa aagaattgat taaggctgga
    13081 gtcgtactca caggcggatc agctctttta cctcatatag acgactgtgc acggtcggta
    13141 ttcggagttc ctgtaagaat aggagcacca gcagccttgc ccggccttga tgttggtttt
    13201 cgttcaccag cattctctgc ggctgtaggt atagtattgc tagaggcaga aagaattgga
    13261 ataccagaac cttcgacgag aaaaaaagcg cgaaaaggca aagaaggtgg cttttcgctc
    13321 ataaaatgga taaaagacag gtttttctaa tgctcgctta ggaggaaaaa tatggcgata
    13381 gaacttgttg atgacaatgg tataggaagc ccaactgtga tcaaggttat cggaactgga
    13441 ggcggaggct cgaatgctgt aaatcgaatg attatggcag gattgaaaaa tgtgcaattc
    13501 atagcggtaa ataccgattt gcaggatcta ggtcgttcaa aagcagaaat aaggcttgga
    13561 atagggtcca aaatcacgaa agggctcgga gctggaggaa aaccagaaat aggagagaag
    13621 gctgccatag aagacagaga taagatcgag caagcgctcc gcggggctga catggtcttt
    13681 gttacagcag gtcttggtgg tggcacgggt acaggttctg ctccaataat tgcgcaggca
    13741 gctagagatc ttggagcctt gacagttgca gttgttacaa aaccattcgc ttttgaaggc
    13801 aagatcataa atagaatagc agaagaaggc ttttcgaagc taaagcaagc cgctgatact
    13861 gtgatcgtta tacctaatca aaatcttctt agggttgttg ataaaaaaac tccattaaaa
    13921 gaagctttta gaattgctga cgaagtactg aggcaaggag ttcagggaat ttctgatctt
    13981 ataacacttg ctggagacat caatatagac tttgccgacg tccgaaccgt aatggaggga
    14041 cagggcgatg caataatggg tatcggaata ggtagcggag acaatcgcgc tcaagaagct
    14101 tctgagaagg cgatcaataa tcctcttctc gatgatgcat gcatagaggg tgctaggaat
    14161 atccttgtaa atgtaacatg cggcgaggat ttttctctca tagaatatca ggaagtcatc
    14221 gacctcatta catcaaaagc tgccgaagac gcacatatta tcgtgggtgt ggtaacagat
    14281 agttcgttaa aagatgaggt gagagttacg gtgattgcga caggctttgg tcccatgcgt
    14341 cagaagtcgg gagaaaaggt aggaacaaaa aataccattg caatgcctct aggatcgatt
    14401 cgcccaagcg aatttcttag cccaggcgaa tggccgctac acgaagaaaa accagctcaa
    14461 aaccgagatc gtgggaacga gaaccaggat aagcccaatt ttagagatag ttttggcaaa
    14521 ataagcttcg atcccgaatc cgataatgat gaattcgata ttccaacagc acttcgcaat
    14581 aagcgcttga tccccgagga tttcggaggc agaaggtgag tattcttgtg cggcgttttc
    14641 aagcatatct gctctctaca aggagacgct ctatacttac tgcggatgca tacattcgcg
    14701 aaatcgagat gctagagagt ttcctttttc agtatggaaa aacgctgctc gatgcaagtg
    14761 aagaagatct tctcacatat ctcattcgca ggtcacagtc aggacttcaa agaacaacta
    14821 tggctcgtat aattgcgagc ttgcactcat tctatcgatt ttgtctaaat gaaaaactca
    14881 gagcagacga tccatcgata caaattcgaa caccgaagca agataggaat ctacctgatg
    14941 tgctcgatcc agattccatc gaacatgtat tggaatgcat tgacattagt tcacccaatg
    15001 gacttaggga cagggcgctt ttcgagctta tatattcttg cggactaagg atatccgagg
    15061 cagcagatct taagtttcaa caactctatc tggaagaaaa aatgctgaga gtcataggaa
    15121 aaagaaaaaa ggaacgtctt gttccatttg gagacgaagc caagtattgg ctgacccgct
    15181 attttacaga ggcaagacct tttttggaaa aacatgagaa atcagatttt atatttctta
    15241 atcaggaggg caaaggcatc tcccgcaagg gaatttggaa gcgatttagt cagatacgcg
    15301 aaaaatcggg agtcaaagcc aaggtccata catttagaca tagctttgcc actcatttgt
    15361 tagcaggagg ggcagatctt cggaccgttc aggaacttct agggcattct gatatagcta
    15421 caacacagat ctacacgcat atcgatgagg aatcgctggg tatgtatcac aaggagtatt
    15481 tcccacggaa atgaaagaag aataaggcaa ggcaacagag aagcaaaaaa atgaaagcgg
    15541 agaacgagat tatatacgct taccctcatg gagggtcctc acaaatggca ctcgagaaaa
    15601 agcatgagaa aaaaggtagg cttatgcaat tggagcttga tcgtatctca gcaattcagc
    15661 cttccatgga taacatttct ctttccctaa agctcgcttt ccaaaggctg cattttctca
    15721 gttggcagga gcgtattctt cttcttgaga atattcgtga catggaggat ttcctagctc
    15781 tgggtcttgc cgaaatcgaa agctttctat accgttcaat gcacgcacga ttattggata
    15841 tgggtaggat atgggaacaa gctgagcgag atgtcaacat attaagtcga ttaggggcaa
    15901 gatttgtctg cattgtagat agagagtatc ctccactttt aagggaaata catagagcac
    15961 catttggcct ttttgtacga ggcgatacaa aggctctgat ccaaccatca gtgactatag
    16021 tgggaacaag gggtcctaca tcgagagggg ttcttgctgc gcggcttctg gcaaaagaat
    16081 cagcagatat aggtgttgtg gtggtgtctg gacttgctcg tgggatagat gctgcggcac
    16141 atcgtggtgc aatacaaagc ggtgctggat ctactattgc cgttctgccc tgtggaccag
    16201 agcgagtcta tccgccatcg aatgcagctt tagcagcggc tattctcgat tgcggaggat
    16261 gcttggttac ggaatatccg ccaggcgtaa gtctagatag atatcggttt ccggaacgaa
    16321 atcgcattct tgcaggattt tccaagctca cactagtcgt agaggctcca gagaaatctg
    16381 gtgcgctcat tacagcggaa tttgctcttg gagaaggtcg ggatgtagtg gtcgccgcct
    16441 cttgccttgg atcttctcga aatgcaggag cggatgctct gaaccaggat ggtgctttgg
    16501 caataaactg tgccgctgaa ctagcctcca tgatccaggg cgaatgtact ggaatataga
    16561 ctttgatgat ttatatcaaa ttcagctgca attgaaggag ttattgaatg gcagcaacga
    16621 gaaaaaaaga aaagaagact cctggagccg ctgagtcggc tgatgcctcc aagctaaaga
    16681 ctcttgtaat cgtcgaatcg cctgcaaagg ctcgtacaat cgaaaaatat cttggttctc
    16741 ggtataaagt tcttgcctct aatggccagg ttatcgatct gccaaaatcg cgaatcgcgg
    16801 tcgatgtagc gcataatttc gagccagaat acattactgt gcgcggaaag gccgaaaagc
    16861 taaaagaact gacagaagaa gctaagcgct cgagagcagt tgttctagca tcagaccctg
    16921 atcgggaagg agaggcaata gcatatcaaa tagggaaata cttagaagaa aaagcaaaaa
    16981 atacaccgat aagcagagtc actttcaatg aaataacaag gcaagccgta caagatgcaa
    17041 tggctaatcc gcgcgacatc gatatgaatc tagtggaggc tcaaaaagcg aggcgcgtaa
    17101 tagataggct agttggttat acgctttcgc cgcttttatg gaaaaaagta aaatcagggc
    17161 tttctgctgg tcgtgtacag tcagttgctc tcaagcttat ctgcgatcga gaaaaagaag
    17221 tagaatcctt catccctgaa gagtattgga ctattgaagc ccatcttaag gctcatcagc
    17281 atattgtacg agccgaacta gctttgctca atggcgataa gccctctatc aagagtgaag
    17341 cagaggcaaa taagattctt gctctttttt ctggaaaatc cgccttggtg agcgatatac
    17401 aatcaataga tcgcacaatt aggccaaagc caccatttac gacttcgaag ctgcagcagg
    17461 ctgcttccaa tagacttgga tttacgagca ctaagacaat gaaaattgcc cagcagctat
    17521 atgaaggcgt agacatgggg catcagcgga ctggactcat cacatatatg aggacggatt
    17581 ctacgaggat ttccgaggcc gcgctccatg agacacatga atggcttaca aaatactttc
    17641 ccaatcagac tccgcatacg cctattcgat attcagtaag caatgcagct caggacgctc
    17701 atgaagcaat acggcctact cgagtggata ttactcccga tgaagcagga cgctacctaa
    17761 gaggcgatca acttaagttg tacgccctca tttgggaaca attcgttgct tctcagatga
    17821 aacccgcagt gattcggacc ttaactgccg acattcaaat tggtgatgga atttttcgta
    17881 attcagcctc tagtttcata gaagaggggt tctataaagt aattcgcctc gcggcttcca
    17941 aggaggaacg aacaagccat tatcttccat tcgagaaagg tgaaatactc ctggtagaaa
    18001 aaattgaaag cgaacaacat ttcactcagg gcccttcgcg ctacacggat gcctcaattg
    18061 tgcgaactct tgaggagcta ggaattggaa ggccttctac ctatgcgcca acgatcgaga
    18121 ctctcattga gcgatattat gtccagagag ataaacgcca acttgtccca acgcagcttg
    18181 gcaaaataat aaatgacatt ctttctaaaa actttccaga agtaatcaat actggattta
    18241 ctgcagagat ggaatctatg ctcgataaag tcgaggagca gaaaattgat tgggtaagtg
    18301 aacttaaaaa gttctatttc cccttggtgg ataaagtcga aaatgctttg aatgccctag
    18361 aggatatgca tggggtattg gatgagaaga ctaatgaaaa gtgcccgatc tgcggtcgtc
    18421 ctctcataaa aaagctagga aggtttgggt attttctatc ttgttcagga ttcccagaat
    18481 gcacattcac taaatccgta ccgctggcta tttgcccaaa atgcggaggc gatatcgttc
    18541 cgcgtgtttc gaataagggt agacgaaaga aattctatgg ctgctcgaac tatcccgaat
    18601 gcagttttaa aaccctttat aagccaacaa atgctacttg cccaaaatgc ggctggtttc
    18661 tcgttgaaaa atacgacaaa aagactggtc attacaaggt ttgcataaac ccagattgtg
    18721 attacctcca ctcaagtcaa cagagtggag acaatagcgg agagtaatga atcatggatg
    18781 cgcgattttt ggactatctt gattaccttg gttctgtgag aggcctttcg cttaggacag
    18841 tggaagcata taggcgggat ctcgcgcaat acgaggtatt tctcgaagga aaagacctcg
    18901 atgaggcaga tgcaagcgat attagaacat ttgctggtac ccttgtgatg gagaaaagag
    18961 cggcttcatc tatcaacagg gctttatctg cggtacgcgg tttttataga tatcggatga
    19021 aatttcatga aaaagggaag gatccagcgc gagaagtaga aaatgtgccg acaggaagaa
    19081 gtcttccatc atttcttttt gaagaagaga tcaatagttt tcttgaaagc atcgagggtg
    19141 aaactttttt agatgtaagg gatagggctc ttttggaggt catgtattct acaggctgcc
    19201 gtgtttcgga actatgtgga atgcggcttt caagattaaa catgaacaac tttacaatcc
    19261 gtgtaaaagg caagggcgct aaggagcgtc ttgtgttcct ctgcgatgca gcaaaccaag
    19321 cgctgagccg atatctgccg tatagagcag ctgttattcg aaagtatgga atcgaagaac
    19381 atgataggat ctttgtcaat tcaagaggtc gaccattgag ttctcgtggt gctgaaaaaa
    19441 tagttgagaa gcggcgtctc aaggctggta ttaggaaaca cctgacacca cacacatttc
    19501 gccatagctt tgcaactcat ttagtggctg ctggtgcaga catgcgcgtt gtacaagaga
    19561 tgcttggtca ttcgaacatc tcaaccacgc aggtttatgc tcatgtcgat atggagcgtt
    19621 tgcgccgggt ttatgaacag gcgcatccgc atggttcaaa gactaaataa gttaaagaga
    19681 ggaaattatg gaaatacatg cgacaacgat actggctgtt agaaaagatg gacatgttgc
    19741 aatggcaggc gatggccagg ttacaatgaa taacactgtg ctcaagagca atgctcgcaa
    19801 aatacgtaca atctataacg gcaaagtgct atgtggcttt gcaggagcga ctgccgatgc
    19861 ctttacgcta tttgaacact tcgaagccaa ggtgcaggaa tatggcggag atctgacgag
    19921 agcctcggtc gagttagcaa aagactggcg cacagatcgc atacttcgca agcttgaggc
    19981 tatgctcctg gtagcggatc aatcgaagat gttcctgctt tccgggacgg gagatgtagt
    20041 agagccaagc gaagatgcca ttgcaatagg ttctggaggt agctatgcat acgcggctgc
    20101 aattgcctac ctcgacgcct cgcgcaggat agaagaggct gcaaaatcca ctgcaagcgg
    20161 agatgccgaa atgctccatg gcagcttttc agcccgtgaa attgcggagc gaagcctaaa
    20221 gattgcctca agcatatgca tatatacgaa tgatcagatt accgtggagg agatatgatt
    20281 actgagaaag acgcgcttac tccccataag atagtggaag aactagatcg gtatatcgtt
    20341 ggccaggaaa aagcaaaaaa ggccgtggcc attgcactgc gcaatcgaat cagacgacaa
    20401 tgcctccctc cagaaattcg agaggagatt gctccaaaga atattcttat gataggaccc
    20461 actggcgtgg gaaaaaccga aattgctcgt cgcctcgcca agctctgcaa tgcacctttt
    20521 cttaaaatag aggccacaaa atacacggaa gtaggctatg ttggccgtga tgttgaatct
    20581 atggttcgag accttatggc agcagccgta tcgatggtta aagaagaaat ggagactcaa
    20641 gtcgagaacg aagcgaagag acgcgcagaa gaaagactgt tggatatcct gcttccaggt
    20701 ctggctaaat ctgatgagcc tattgggcag tctcaagcaa ctttgataat ccctagctct
    20761 gggatggaaa caaaagaaaa atttcgatct ctcttgaggg aaggcaagct agattcgcgg
    20821 cagatcgaaa ttttggtgcc ggcccagggc ccacagatcg agctctttgc tggaacgcag
    20881 ttcgaagaga tgaatatagc tttaggcgga ttgcagggct tatttggtgg taataagaag
    20941 aaaaagacag ttagtgtcgc agaagccttg cctattcttg aagcagaaga aaaagaacat
    21001 ctggtcgacc aggagaagat tgtccaggag gctcgtagac gaactcagga attaggcatc
    21061 attttcgtcg atgagatgga taagattgcg aaccgggaag ctagaggctc ttcggggata
    21121 gacgtttcca gagaaggtgt ccagcgagat cttttgccca tagtcgaagg aacgattgtc
    21181 aacacaaaat gggggccaat cgatactact catattctat tcatcggagc aggagcattc
    21241 actgtagcca agcctcaaga tctcatacca gagctgcaag ggcgcttccc aattcgagta
    21301 gagctagatg ccttgaccgg tgatgatttc gtgagaatac tcactgaacc caagaatgct
    21361 cttgttaccc aatatattca gcttctgggc accgaagatg tgcagcttga gttcactgag
    21421 gatgctatcg caagaattgc cgagatcgca gccgcagcca ataggcaaag cgagaatata
    21481 ggtgcgcgac gcctgcagac ggtcatggaa aggctcctag aagatatttc cttctatgct
    21541 gatcagcatg taggcacaaa agttatcatc gatagagcat atgtggatga gcgcttcaaa
    21601 gactatgtcc aacagcagga tttgagcaaa tacatcctct agtggaaaaa ggaaaggaag
    21661 ggtcatggac tatgcaacat tagaagaaga tcgcttatgg gaactctacc gaagaactgc
    21721 agacatccat atccgcaatg agatcattgt gcgctacgct ccgctagtca aatatgtagc
    21781 tggaaaagta tctgtaacct tgccttcgat ggtggaatat cgagaccttg tcggatatgg
    21841 gacatttggg ctccttgatg caatagaaaa attcgatcct caaaagcatg taaaattcaa
    21901 gacttatgct gttacacgga tacgtggagc tatatacgat catttaaggg agttggactg
    21961 ggtacctcgt tccatacgcc gaaagaccaa gcagatagag cgcgctattg ctaaattaga
    22021 gagtcagctc gggcgacctg ccaacgatga agagatagcg aaagagatgg ggatagagcg
    22081 caaagaactc ttgaagataa tggctaaagt agcgtcttcc tcaattcttt ccttgcagga
    22141 gatatggcca atgaattccg aagcagaggg atataccata gaagattctc tcgaatcgat
    22201 gcgaattgaa aatcccgact tggtactcga acgtgaagaa atccgtaaag tcgtattcga
    22261 ggctctccag gagctacccg agaaagaaaa aaaagttatg gtcctatact actatgagaa
    22321 tctgacctta cgcgaaatag gcaagatact tgaggtaaca gaatctaggg tgagccagct
    22381 ccatactaag gctattttgc gccttcgtgc taaactgtcg aacaagaaac gtggcataat
    22441 ataagtaagc ataaaaagtt tatattgaat caagaattct gttgaatttc tgttttttct
    22501 ctgctactgg aagaggaaga agagctggcc tcatgttttc caatcccata ttgttctatt
    22561 ttttgataga gtgtctttcg gcctatttta agtatctcgg cagctttaga cttgtttcct
    22621 cctaccagcg caagcgtttc tgcaatgata attcgctcgg cttcttccaa ggatgaaaag
    22681 gctggtatac tgatagtccg agcttctccg ctcgagcgcg gtcctggagg aaggtcatcg
    22741 agagttatca gcttgccaga agccataacc actgcacttt cgatgcaatt cctcagttcg
    22801 cggacattac ccggccagtc gtaggtatag agagcctgcc tgacttttgg atcgaagccc
    22861 tcaatctgct tgccattctc atctgcaaac tctctcaaga aagccatggc tagaagtgga
    22921 atgtcttctc tgcgttcgcg caatggaggc acatgaatgt tgactacatt cagacgataa
    22981 tataagtctt ctctaaagcg gccttcggct atttcttttt taagatcacg gttggtggca
    23041 gcaattaggc gcacatctac ttctatggtt tcttcgccac ctactctttc gaactttcgc
    23101 tcttgaagaa cacgtagaat cttgatctgg acattctgat tgatttcgcc gatttcatcg
    23161 aggaaaagtg ttcccccgtc agccatttcg aagcgcccgc gctttctacc ggttgctcct
    23221 gtaaaagcac ctttttcatg tccaaagagc tcgctctcga gaagactctc agcgagagcg
    23281 gcacagtgta cctttataaa aggcttgtct ttgcgtggtg ataagttatg gatcgcatca
    23341 gcgacaagtt cttttccgac acctgattcg ccagttatta gcacacttgc cttggtcgga
    23401 gcgacctttc gtataagatc gaaaatgtgt ttgatagaag gagccttacc aatgatgtag
    23461 ctacgagctc gctggctttc gacttcggcc tttagttcga tgttggttcg atagagctct
    23521 cttgtctcca aagcgcggcg gacaagattg attagatgat cggtatttgg cggtttggcg
    23581 ataaaatcga atgcgccttc tctcattgct tccacagcct tatcgacact accatgagcc
    23641 gtgagtatta ttacaggaat gcctgggtga gtgctcacga tataatgcag aagatcgagg
    23701 ccactcattc tacccatttt gtaatcggtt atgacaacat cgatatcttc tttatcgacg
    23761 atcatcttcc cctctatgcc gtcttttgca gttcttacat cgtagccttc catggaaaaa
    23821 agctgggcga gtccttcacg tatgttggtc tcatcgtcta caacaagcag tgtcgctcgc
    23881 atttattcct ccctctccga accttcgatc tgtatattat cttcccatgg tggtaaggat
    23941 ttttgctcag gctgaggtat aggcaagtgg attgtaaagg ttgatccttt tgatggcttg
    24001 gattctactg tgatttcgcc attgtgttct tttataatct tgaatgtgat cgtaagccct
    24061 agaccagtac cactttcctt tgttgtaaaa taaggctcga agatcttggc aaggagttct
    24121 tctggaattc caatgcccgt atcagagata gatatttgaa gctcatcgtc ttttgtcacc
    24181 gccgaaattg tcagagttcc accatctggc attgctgcaa tagcattctt aacaatattg
    24241 agcagggcct gtttgagatg cttacggtct atcataacct caggaaggtt atcttcgaat
    24301 tttgttacga tattgatatg ttgctcagag gcttcattgc ccattatttc cacaacttcc
    24361 ttaatcacat cttctatgct atcgcgaata agagttatat ccatgggacg aacggcaaat
    24421 agaaaatcga ctacgatcga attgagtcga tcaatttcct gttccactaa agaaatgtta
    24481 ttgaaaatag gctctgtttt cttgttgtat tttttttcga gtatgcgtcg aataatgcca
    24541 agttgaattc gtattgaagc aaggggattc tttatctcat gagcaactcc agccgctagg
    24601 gttgtaagag cggccaggct ctctgctcta cggagctgag cttcgcggcg gcgcttttct
    24661 gtaatatctt cgatgtgaat tagattacca gctattttcc cttcagaaac caatgtagat
    24721 atgctgatgg atagcaatct cgttccccct ggcctgccga gagagagttc acggttcaat
    24781 atattctttt cagatttaag tgcttcctgg ataaaatcag ccagattgtc atcattgagc
    24841 tgttcccaaa gtacagtttc tggcgtccat gatacgattc ttgtgatttt ttctgcggag
    24901 cgattgataa agagtggttt atgtgccaga tcgcatacga tgattccatc tagtgtcgaa
    24961 ttcaaagcag attcatagat cgagttctct gctaataggt tttttattat ctgtttgagg
    25021 ccttctggag gaatacgatc gatgcgctta agtgtgtttt catatatggc tttcatattg
    25081 ctctccaaaa atatctgcaa acgcttcgag gaggagttcc ggcgaccgat tcaatgtgcg
    25141 atattgcatg ttcatttccc tcagctttga aaaaagacga tctacataca tggtaagcga
    25201 agcgctcgag cttggatcag tagctataga agtcaaatgt tgagcaaccc ctcttatgaa
    25261 aacaagaaat gaatttgtaa atttagaatt cttggcacca aagccattcg ttttttctga
    25321 tacttgtcga agcatcgata gagtagagac attctggccc tgcgccatcg aggcgagttc
    25381 aagggcacgt tctccactca atccagcatc cttttccatg gcttttccca gcaaagcacc
    25441 tacaaataaa gcagcatcct ggatggcgat cttgggcgga taagcgctct tgttctgaaa
    25501 gaattcgctt atgctgttta tttcttgtat atcttcattt gcatgaaata ggcgagagat
    25561 gatttcgcgc gcttgataat cggttcgagg gtcaaatgat atgagtcttg atcttgaaag
    25621 aatggtcgcg atgattgttg cacggctaga tgaagtcaga ataaagcgag ctgtctctgg
    25681 aggctcctcc aggatcttga gcattgcgtt tcgcgaactt tcattggcag catcggcatt
    25741 ttggattatg atcatcttag tttttgcaga tggagcaagc actgcccatg aaatcatatt
    25801 tctaatcatg aaaattggaa taccatctgg cactagagat tctagtccag ctgctgcatt
    25861 taccgcacta tctaccaatg cactcaagcc atggtttttt ttcttcagca cttgttctaa
    25921 aatctcttgt agcatctcct cgcacgtttc tatagattgt attgcttttg atagtcttgc
    25981 ttcttctcct tgccaaagga cagaatcaaa gcgttttagg agttttctta tcgagcgcgc
    26041 aaaagcgtaa tatgaagtca agttgggttt tctgatcaaa tattctcctg taacaattgt
    26101 ttcctgaggg aattctcttg gtccaaagag caaaaggtca ggatgcataa ggtttctatg
    26161 acaattgcat tgtatacatt ggcaattcca ttctcctgcc ttttcgcaag acaagactcg
    26221 agcgcattcg agggctgcag tcagctttcc tgatccagca ggaccgacaa agaggagggc
    26281 gggtggtacc ttcttttctt ttgccagcga gacaagctgt tctagagcat gattttgaaa
    26341 aagcagattc tcaaacatgg tcatttacct ctaggattct gcttaaggct tgggatctcc
    26401 ccactcttta tcattggggt tagcgttaca cctatggcag gttcgatgat cggtcctaga
    26461 agtcttacat agagcgagtt ggagagtatt gcctttacat caactatagg ctggatctca
    26521 agaataacaa taaagagacc cacaacaata aaaccttctg caacacccag aacaaggcct
    26581 agaattctat ctagtatgtc aagtctggat gcctcaagcc cttcgcgaag catgcgctct
    26641 attattttca ttagaagaaa tcccgctatg aagcacagaa taaaagcaat aatatactga
    26701 acctgataag gaagcttgtc ggctttgatc ttcggcaata tctgcaggat gacagtgttc
    26761 gaaagcaata agccaacaag tattcccaca gcgatagaag ctacagataa aatttcatgg
    26821 ataaagcccc taacaaagca tcgtgctgcg agtattatca caaccccgct gaaaatccag
    26881 tctattgtgc tcatgcagag tcctttgctc cttctgcaat tttttttaga aggagccgag
    26941 ctatgtattc cgccgagaac atgggatttt gacttaaagt atcagaaact tcagcaatat
    27001 aaggagtatt ggcaatgcgc acaaagctat ggcaggcctc ttctgccata gcatagttct
    27061 cgaaatccag atatttcttg actgcatcgg caaataaatc cgatggaata tttggcccct
    27121 gcagaacggt tgctatgccc gaacgagcag caatagcagc attttccact tgatcgccgc
    27181 gagtgcctga tcctgacaaa ggaataagga tcataggctt accaagagaa gctgcttccc
    27241 atatcgcgcc tgcgccagcc cttcctgcta ttatagtcga agcagcatag atgtctgcaa
    27301 tttctgaacc tatataaggt aaaggacggt atgctttgcg gacatctgga tcatctggaa
    27361 ttttcgcctt gacttcgtcg aaattggctc taccagtttg gtgtactatt tgaactaatg
    27421 gtacgagcag actaagcgag gagagcacaa tatcatttac ctcctgcgca ccctggcttc
    27481 cccctagtac aaataaaact ggcttttcag ccacgaattc gagcatggat agtccacgct
    27541 gtcggtctcc attaaggata tcgggtcgca ctggatttcc ggtttttatt atcttgctcc
    27601 taaggctctc aggaaaatat tgagctgtca cttcccagct tgtaaggata agcgatgctt
    27661 tcttgctgtt tagtctagtt gcaagtcctg gagaagcatc tgattcatga gtaatgactg
    27721 gaattccaag ggaggctgcg gccctgcaag gtggaacgct cacatagcct cctttggaaa
    27781 aaagaatcga tgggtgaata tctttgagaa tatcccgcgc cctgaaatag gctgaagcta
    27841 ttctccctag atccgagatg ttgcgaagcg aaagcttacg gcgaaatttt cctgaaggaa
    27901 ttgcataata ttcgatgcct tgtccaataa caatgctgcg gtctaattct ttttcggagc
    27961 ctatccagac aattcttccc ttgaagccaa gtttacgtaa ttcctcgata accgccaagc
    28021 ctgggtatat atgacctcct gtgcctccgc cggtaaaagc aatgcaggct ttttcatgtt
    28081 catccttcat ctggggctct gttttatgaa tagactgcat cttaccttct cccatagata
    28141 tcgcctaatc caatcagctt aatctccatg atttttgacc gcactcaaag cagaaacaat
    28201 gaaagcatct aataattcat cgtcagtgga ttcgccttta ctgatagcat taagagcctt
    28261 ttttgcaagc acttttccta attgtacgcc ttcctgatca aaactgttga gattccacac
    28321 aaagccctga aacataactt tattctcgaa atgagaatag agagcgccaa gctgtcttgg
    28381 tccgagttct tgggccaata gaagtgtcga tggacgattc cctgggaaag ctttgtttag
    28441 gttttcatct tttttgccaa ctgcaaatgc atggatttga gcaatgagat tggccaagag
    28501 tttttgacga gagctcgaac cttcagaaat catatcctgg gaaagttgat ttctgagaaa
    28561 acctatgaat tgaagaggaa tagtatcagt tccctgatgc agaaactggt agaaggaatg
    28621 ctgaccatta gtaccaggtt cgccgaatac taaaggccca gttgagtaag aaatgggatg
    28681 gccttcgcgg tttacagact tgccgttaga ctccatatcg agctgttgca agtgcgctgg
    28741 gaagcgagaa agggattcag tatatggcaa aacagctgtg gctggccaac ccaatatatt
    28801 tctttcatag atgccgatca atgcatccat gagcatagga ttttttctaa aggaaggttc
    28861 aaaggctagt ctatcagcct catttgctcc ttccaaaaat tcttcaaagg tttctgggcc
    28921 ataggcggtc gagattataa cccctcccac tgcgctagat gtcgaatatc gtccgcctat
    28981 aaaatcgtct atatagaaac tatcaagata atgcgggtta tgagccaaag ggctagaagt
    29041 gcttgtcaca gccacaatat gcttatctgg attcagtcca gccctctgga gcctatcgcg
    29101 cactagggtc tcgttggcca gtgtctcttg tgtggttcca ctctttgaca caagtacaaa
    29161 cagacttttt gagagatcaa tatttgcaag cacataatcg gcatcatcgg gatcgacatt
    29221 cgaaatgaac cacggtctga gcctagcgcg cccctttgca gccgcccagc gagaaatggc
    29281 tagacttgcg gctcttggac caagatccga tcctcctatg cctatctgta caatttcggt
    29341 aaaggccttg tccgcagagc taagaatttt gccttctcga atcttttttg cgaaatcgta
    29401 gaatcgatgc ttttcttctc gatagaaaac ttctaaatcc tggccattca agatcacggg
    29461 tttgccgagc tgtcctcgag ccaagtgatg gagtaccatc cttttttcac ttgtgtttat
    29521 gattgcaccc tctgtcagca ttcgatactt ttctattagc tgctgttccc ttgctagagc
    29581 ttcgagttcg tcgattatcg catcatctac aggtgcaaaa gcatagcagt agtaaaaacc
    29641 tgcggcatga ggaatccacc agtccgatat ccgctctttt ttaagagatt cgcgcaattc
    29701 tatagctggc atttttttta gtacttcaaa ggcctggcaa gtatcaaggt ttttaaaaag
    29761 aaccataatg acctctcggt tcaaagtctt tcttgttgag gtgtttcttt ctcatacatg
    29821 atattgctaa aagtgataca cttcaagctc acccaagact gttttgagca agtctgctga
    29881 gggttttgcc ttttttacta agcagaatcc tgtttttcca tgttcccttt tcaggaacat
    29941 ataagatatc tagtgatgat agagaattga tcaattcttc tctttctatc ttgagccatc
    30001 catagagaca gccagcatat ttcccttttc ctttaccata agctccaagt gcatctatta
    30061 gttcttgggg agtaaagaca ccttgatgcg aggcacagaa agaaatagtc tcgataaacc
    30121 cttctgtctt agatagaaat tcccattctg gagggatatc tgaagcatct aaacctaatt
    30181 gcattttcag agtatgggat ccttcgatac aattatcgca ttttccgcac tctggcgttt
    30241 gttgctcgcc taaaagatga agcaggaatt ctcttcggca tgatctcaga gtgggatagg
    30301 gcaaaaaaca agcttttctg atcgactgta tgtctttctc tttatcgtcc ttcttatttg
    30361 ccttgtgata ttgcgaatat ggagctgcga gatcatcgat gaggatagcc aaagcattct
    30421 ttccgtcgcg gcctccgcga ccagcttctt gaatatatgc ttcggcactt tctggtagag
    30481 agctatggat aacagttctg atgttttttt tatccatgcc cattccataa gcatttgtcg
    30541 aaaccaagat cgcatcgtct gagttttgaa accattgttc aatactgcag cgctcgactc
    30601 gatcgagccc agcatggtaa aagcgtgttt gatagaatcc agaagctttg agcagctcag
    30661 ctttgagcct tgttcctgga cgtgaacgat cgaaaataat agctggtttt ttgcagcaca
    30721 acagggtctg acggagagtt ctggttatag aaagtgtatg cacaacagca taaagaatgt
    30781 tagggcggtc tgcctctgca tctactaatt gataattctt atccataaat agtttcttct
    30841 cgatcgattg tacgatatga gggccagctg tggcagtgaa agcgctcaaa atcctaggcg
    30901 aaattgcctc aacggcctcg ccaagtgcaa ggtaggacgg cctaaaactc tctccccact
    30961 cgcttataca gtgcgcttca tcgactacaa agtgagcaat tctaaattgt ttcaagaatc
    31021 ggcgaagttt tggtgcactc aatatctcag gattgacaac gaccatagta gcttttcctg
    31081 aagtgatcag tgtttcctgt tcatgccatt cttccttact aagacctccg cggaaaagtg
    31141 cgcagggaat atttgcctca tcgagtctgc gcttttggtc attcataagg gcaagaagag
    31201 gatagacaac tatagtcagt ccatctaaaa gcaatgaggg aagctgaaag cagactgatt
    31261 tgccaaatcc agtcgggaaa aggataagct gacgataggt ctcttgcctt tctgtaatct
    31321 cagccttttc atcttggttc actattgggt cgtcgacaat aagatttaaa ctgggatctc
    31381 cgtctaactc tgtagaacct ttaatttcat ccaatatgtt tgcaatcaca taccgctgga
    31441 gaggagtcag atgtgggata tgaaatcttt cacgcccaag aactccaata gggtcttcag
    31501 gttcagaagc gagatccagt ttgagcattt ttggctttca attagtagac gctctattca
    31561 ttgcctttgc ttcaataaat tggataagcc tattttggga catagatcta cgacaggaca
    31621 gtacacttct tgcaatgtac atactggctt gcgcgcggtg caagtgaatt ttccatgcct
    31681 attcactgca tgtgaaaatt ttgtatgctt ttctggtgga agatttgctc ggatgatcga
    31741 ttctataaga gtcgcttctt tttttgttga tactccgagc ctaaaaagaa ctctaatcac
    31801 atgcgtatct acaataatgc caggcttttc ataacaagct gagataagca gatttgcaga
    31861 ctttcttccc acaccaggca attcgatcat ttcttcaaaa ctcgatggaa tttgcccatt
    31921 gaatcgctcc ttgagtactt tggctgtctc gattatgtgc ttcgctttta cacgatagaa
    31981 gcctagagag tgaattatat tctctacttc atgtatattt gcatctgcaa gactttcggc
    32041 attcggaaag cgacgaaaaa gctctttcgt cacaatattg acctgatcat ccgtggtctg
    32101 ggcgctcaaa atgacggcta taagcaactc aaagcaactg cgataatgca aaagagggcg
    32161 agtatcgggc catattggaa ccagacgaat gtataactct tttagcaact gaggaagggt
    32221 catcgattca tttttgtctt ttaaggttat tcctagttca ctgctcatta ttatctcctg
    32281 tctcaaactt gacctttctc tattcaccat atagtatatg gcggctctaa tttatcgcaa
    32341 tgagagggag gactcatgaa tccatctatt ctgaaaggaa gatcgctcct cacatggatc
    32401 gattatacac cagaagaaat aaggtatttt ttaagtttat cgaaaaaagt caaagaagaa
    32461 gcaaaaaaag gcatgcataa gcagcg
//
LOCUS       DAOSOB010000016        30776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1499_length_30776_cov_66.237232, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000016 DAOSOB010000000
VERSION     DAOSOB010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 30776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30776
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1499_length_30776_cov_66.237232"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..2650
                     /locus_tag="PLS88_02995"
     CDS             86..2650
                     /locus_tag="PLS88_02995"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOU06348.1"
                     /translation="MPEQFLYQQLDTLSQWGSIEKEVPDSVARNLNPKYELRPYQIEA
                     FARFFHCYRNDFPNKTYPLHLLFNMATGSGKTLIMAGLILYLYEQGYRNFLFFVNSTN
                     IIEKTKDNFLNPSSIKYLFNPDIFIDKRHIHVSSVENFEAVNTNDINICFTTIQKLHS
                     DLTNEKENSLTFEDFRKHKIVLIADEAHHINVKTKSEGELFESWENTVERIFTQNEDN
                     LLLEFTATHDYATPTMVKKYRNKVIIKYDLLQFRNDRFSKDVVIVQSDFDQQERILQA
                     LILSQYKQEVAAKYRINLKPVILFKAQRTIEQSKKVQAEFHKLVDELTARQIAGIRKS
                     NIPLVQRAFRFFDEQRISDEQLANRLKQEFQPDFCLSVNDEKEKENYQILVNTLEDKN
                     NRIRAIFAVQKLNEGWDVLNLFDIVRCYETRDSGKGKIGTTTLSEAQLIGRGARYFPF
                     VLPGSDDRFRRKFDGDLGHELRVLEELHYHSINDSRYISEIRQALIEQGMMDEREVTR
                     ELKLKESFKETPFYKYGIVWLNDRQRKDYQRIYSFADLGVKKRNYIHCIATGQGGTTA
                     ALKNAEKELMWKDEACQDVKVRDIERNIVQSAIARNPFFTFANLKHYFPHLVSMHDFI
                     ASYDYLGGLEITFQGNVYGLGYNRAEQLAAMSGLLNQIESEIRANVTDYEGTHEFRHD
                     WVHEVFKDKILKFNAQNPRAADDAQFEYFVSTKDWFAFNTIYGTSEEKAFIRMFDRQM
                     EKLQEQYEQIYLLRNEGHFAIYNYADGQAFQPDFVLFLREKGGKLLIYQLFIEQKGRH
                     IEEYDRWKEIFLEEITSEFGSKSLTFEGKKYRLIGLPFYKKEDENQFIARFESVLN"
     gene            complement(2859..2930)
                     /locus_tag="PLS88_03000"
     tRNA            complement(2859..2930)
                     /locus_tag="PLS88_03000"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2895..2897),aa:Glu,seq:ctc)
     gene            complement(2999..3069)
                     /locus_tag="PLS88_03005"
     tRNA            complement(2999..3069)
                     /locus_tag="PLS88_03005"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3035..3037),aa:Gln,seq:ctg)
     gene            complement(3122..5317)
                     /gene="recJ"
                     /locus_tag="PLS88_03010"
     CDS             complement(3122..5317)
                     /gene="recJ"
                     /locus_tag="PLS88_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOU06349.1"
                     /translation="MKWIKREIDPVQVRSLARRYEIDTLTASILVRRNIIEPEQIQFY
                     LESDLQLLHNPFLFKDMEDAVDRIITAREEEEKVLVFGDSDADGITSTVLMTEALRDF
                     GLKVLQKVPEGEEPYGLSKASIDFAEEQDVSLIVTVDCGISNHEEVLYASQKGIDVII
                     TDHHHIQAEEPPKAIAIINPKIPDCGYPFRDLSGCGVALKLAHALSIARLGILKEPLA
                     LLRAGQAIHQEICLNIEKNKLNTEKEGNLEIEAIRLDNLIETSRLRIISDSRGYFPSG
                     TLEKLEKFLRGRIVVAWNKDTIEAFFKKNFAGLVELEVLDLEKVVTPIWPNMSANSLE
                     ELVQISRLKKYAYGPYSTIDTLKHIFNGYLLYTLQSQSLLSDRMLQLAALGTIADLMP
                     LKNENRLIVRKGIESLNTAPTNGIRELKIVLNLGRPLGSNDVAWQITPIINAAGRLGK
                     PKLALDLLQAETTNEAIEAASQLIQANNERRRLGAEAWEVIQDQLAESLKKSGGKFAV
                     VGSSKIKSGITGLLASRAANLMKIPVIVAVFKTDGTCTGSIRSGDTFPLTQLLAFSSD
                     LFLDYGGHDSAAGFTMKTDDWQAFLERIAQFMAHTELITEESELSIDAELPHSFVTPD
                     LLQLCRQFEPIGEESKPLVFYSRNVSMVDAQIVGKNGKSHLKLTLDFGTYKWPAMLWD
                     GAQRLERDFSFRNNDRIDIIYKVTTNYWNGEERPQLELYDIHRADLKGL"
     gene            complement(5314..6624)
                     /locus_tag="PLS88_03015"
     CDS             complement(5314..6624)
                     /locus_tag="PLS88_03015"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOU06350.1"
                     /translation="MRAVDIIIKKRHGESLDEKEIRFFIEGYVKGEIPDYQVSALLMA
                     IFFQGMSAQETAALTQAMLDSGERMDLSGIPGPFIDKHSTGGVGDKISLPLAPIVAAC
                     GVRVPMMSGRALGHTGGTLDKLESIPGYITDLEPAEFRKGLLEEGFAMTGQTAHIVPA
                     DKKLYALRDVTGTIESIPLITASILSKKVAEGAEGIVFDVKCGSGAFMKGYDDAKALA
                     KSLVATGKKMGKKVVAVLTNMSQPLGYKVGNFLEIEEALDCLEGKGPKDVMELTYWLG
                     AWMLVLGGKAKDVSEGKSLCEEAIESKAALNLFYRNVVRQGGNLEEMQKKRGSWRSEY
                     SMEIRADKDGYIASIDALKIGLAGVYLGVGRNRTEDSVSPTAGFIFDKKSGYHVSKGE
                     RIATLFGKDSQSLEAAFPLALNAISIENTAPSISSLILEEITVS"
     gene            complement(6688..7479)
                     /gene="recO"
                     /locus_tag="PLS88_03020"
     CDS             complement(6688..7479)
                     /gene="recO"
                     /locus_tag="PLS88_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOU06351.1"
                     /translation="MAQRNERYRGLFLHAKDTARGDRILTFLCNEGLLSLFMFGGPKS
                     SLRSAALPFIVADVEVYHDTRREFIKLTGANVFEVFDGINKSFSHMQSACAASEFLIR
                     TSAFGGEYPQALSMITALLRELANVDESEALLVLSAFFWNALSPLGLIPDLLSCERCG
                     RSFSEKNSLPGKLLINESSFLCHNCAEEFRQMGLSTSAMLPFDEKVLTCLRSITKLSY
                     SEALPLLKSASLRPEIIAIIESLAESAAEGPLQSLRVFGETFYGT"
     gene            complement(7483..8211)
                     /locus_tag="PLS88_03025"
     CDS             complement(7483..8211)
                     /locus_tag="PLS88_03025"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU06352.1"
                     /translation="MIPIGDDNTCRRGNAVINWILIALNILVFIFIQKFGLDDYVTLA
                     LAAIPEEILSGRRLFTLITSQFTHAGFAHLIGNMMFLGIFGDNVECRIGKLRYILLYL
                     ISGTIGMLLQILLAALAGGAALQMPLVGASAAISGVLATYLVLFPGNRVVVLLFYFIP
                     TTFSAWFVIVTWFLMQILGGLSGLTAIQNGGTAYFAHIGGFLSAYLWGKSYKKKELKR
                     IAEWRKSRTAGYSDGFHWWIVDDD"
     gene            8322..8972
                     /locus_tag="PLS88_03030"
     CDS             8322..8972
                     /locus_tag="PLS88_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOU06353.1"
                     /translation="MISALLFDLDNTLYSEATGLENGVSQRINHFIADFFRLSIPEAI
                     RFRREHAKAYGTTLEWLMRDYGFQEPEKYFRTIHPEDEADCLNPDMVLKLMLNKIPIP
                     KAVLTNAPMEHAIRILDKLGIMECFSGIYDIWFNNLIGKPNSDSYINVLEAAGFDREQ
                     TLFIDDLPKYVKGYVDIGGPAVLKDEMDRFADLPFRRLKTIYELDKVLDEFNSSYM"
     gene            complement(8983..9867)
                     /locus_tag="PLS88_03035"
     CDS             complement(8983..9867)
                     /locus_tag="PLS88_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06354.1"
                     /translation="MNFSPIVLIDFLKYTIAAATPLLFASIGGLFSELSGMLNIALEG
                     LMAIGAFFGMAAAGLTSNIFFGALAAILSASLASMLMGLMINKFRTNLFITGLAINLL
                     AQGITAVLSQSWYHTKAVVAFNISVDNHLFPRFISRLPLIGPIFFSHSMITIFAWLWV
                     LTSAFIIKNTKHGLKIRATGSNPQAVSALGFSPDRIHMQAFLWSGVGSGLGGYALGLS
                     ISAFVPNIISGRGWIALVAIYLGRKKPLWIAASCLLFAFAESLSNYIQGFSSIPPLLV
                     LALPYLVTLISLILAERE"
     gene            complement(9867..10901)
                     /locus_tag="PLS88_03040"
     CDS             complement(9867..10901)
                     /locus_tag="PLS88_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06355.1"
                     /translation="MRNQRAFSATFRVLMAAAFSIILAATVLLLFAEDFRSALWQFFV
                     EPFSSRYFFGNYIADTVPLIISGIGVSIAFRSKNFNLGGEGQIYAGALAGGVLALHAP
                     EAFGPGTVWILVAFIGAFIGAIIGTISGLLKAYWRISEMISSYLISAIIINISDFFIT
                     GPLQDSTSNFQTTAQIPSVFLLPRIFSPSKLDISIWLALFLVLFFHILSKKSKMGFEL
                     RVCGENEEFARYCGISVRAYNIAAMAISGGMYGLSGALLVIGPQGRVMRGFSSGLGWS
                     AISVALLAQGRETAILPAALFIAFLKAGSDHVMIGSGVPSEIISMLQATAMLFITAQV
                     MPKLWQRQRQ"
     gene            complement(10898..12484)
                     /locus_tag="PLS88_03045"
     CDS             complement(10898..12484)
                     /locus_tag="PLS88_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU06356.1"
                     /translation="MLAVRMQGICRRYYPRGVHANDGASLEVEKGSIHAIVGENGAGK
                     TTLMKILAGLDLPDEGSIEIEGRLVKIESPSKAQALGIGMVQQHFLIFDELSAAENIF
                     FGIEPIRNNKISRLAGIVDSQELNTRAQQLATEYGFAIDPSAPAASLSIGARQQIEIL
                     RQLARHAKILILDEPSSVLTEQETRVLFEKLSDIRRLGHTIILVTHKIHEVMQIADRV
                     TIMRQGRTIGTYDISATTLQKLTSLIMGEGSIEEKQEPIEKSQIDKSKIERSQKNPLV
                     LGVDGMTVRRPRYYGIGLSDISFEVHAGEILGICALSGNGLDDLENALAAFQSPSFGT
                     FIFDGHHIEPHNYRRYQSLFQKGLIAYLPSDRIKRGLASSLSVRDNFIALSRHDFFPK
                     GWILENAAQQAILDAIRSFEINALPDQPAGELSGGNIQKLAIARLFSRDFPKLFILCE
                     PTWSLDIRSAEQTCRRIRLAKEEGTAILLLSSNIDEILELSDHIMVLSRGRRVLYQKN
                     SSLLNRAIIGEYLLGNRIHG"
     gene            complement(12495..13499)
                     /locus_tag="PLS88_03050"
     CDS             complement(12495..13499)
                     /locus_tag="PLS88_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU06357.1"
                     /translation="MRRFMIASLVVILASTALWAQAPRIGVFIPGARDGSPIYDNMAK
                     GAERLVSETPGASIKVIEAGFNQAEWQEKLISFVASGKFDIVLTSNPSMPELVNEASK
                     LFPKQKFICLDGYLIGNSNVYSALYNQLEQGYITGYLSGLVSTSGMKGTNPEKKIGMI
                     IGQNYPAMEKMIIPGILKGLRAIDPAFQLDIRVLGNWFDAAKAAELSRSLYAQGADII
                     LPICGSASQGAVRVAQETGKYLVFFDDDEFARAPDNILGCTILHQEELAYRSLKAAIE
                     GKLPFGKAEIVGMKEGYIEFLNKNPSYIKNVPESIRIKVDAVIQSIKSGQLTFAVPSL
                     "
     gene            13634..14104
                     /locus_tag="PLS88_03055"
     CDS             13634..14104
                     /locus_tag="PLS88_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU06358.1"
                     /translation="MAEKLLKIGELASLFGVTARTIRYYEELGLIEASNRTEGLHRRY
                     PADAIVRLKRIEELKSLDLTLGQIREFFILFSEDPSGEKCRLLLIQSYEDQKKEEETR
                     IKEAQNRIAQIEANIITIKEKKSFFSCPGEECKVCNFNGFCVDSEHNSDQEMKG"
     gene            14249..15724
                     /locus_tag="PLS88_03060"
     CDS             14249..15724
                     /locus_tag="PLS88_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOU06359.1"
                     /translation="MILAIDIGTTRIKAALFDSEGNSLKLELLDLPDEGSNSYQEIDA
                     RVWLKAFSEIISRLISAGEQNDLRAIVICGNGPTILPIDEKGEPIAPAITWLDRRAQK
                     EAKEASIALNYSLDAAFNLPKILWMKRQTPNIYEKANLFVSCPEFIAGRLTGEWTTCL
                     PNQGYKRIIWDRRALEMLELDVEKFPRFIGVGEIVGHIHDYAAKEFGLPKGLPVVMAG
                     PDFIASLLGTATVKPGRTCDKAGTSEGINLCSERDIGDVEALLVMPHIIVPYFNISGV
                     ISSSGIALSWFRSRFLPKEDFETIYSRVASTKPGAEGLVFLPYLAGERSPHWDPNARA
                     VFLGLGLHHDLNMMARAVLEGTAFAIREVLDVMESSGASVKDMRATGTPSLSPVWNQI
                     KADITRKRLMVSSFPEPELAGCLAIGRFALGEESTLTEAAERVFIPAEIFEPRNDTES
                     MYCDLYEVYIDSYRRLADLFPAISGLQKSAIVQQGPFGKGF"
     gene            15903..16484
                     /gene="pduL"
                     /locus_tag="PLS88_03065"
     CDS             15903..16484
                     /gene="pduL"
                     /locus_tag="PLS88_03065"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: protein motif:HMM:NF011652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOU06360.1"
                     /translation="MPKMDIIINLSNHHIHVNHEDLEILFGKGHELTKTKDLIQPGQF
                     ACAETVTVKGPKGQLEGVRILGPERKETQCEILMSDVFKLGVQGCPVCESGQLEGSFP
                     FEVIGPAGSIKKERGLIIAKRHIHFDIETAKHFGVSDKQIVKLKAGEERGAILLNVVC
                     RVHPTYALECHIDFDEGNALGITSGAIGEIVQC"
     gene            16540..17610
                     /locus_tag="PLS88_03070"
     CDS             16540..17610
                     /locus_tag="PLS88_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOU06361.1"
                     /translation="MNKFILKATDLDGYLMESDLEYLSRMNELYEQAMASFKILSTST
                     SEAQKRHEEDILIELYNQMGKLMQEICASEPRIQVYSFVSPQETHGEVSRLIAKLRDV
                     NTGHQEFVYYIQRAFELLFTLAFGVAGKTNKNYLIVRTPVNIPVQNYAVHKIPNVDSA
                     VHDTVMCVMLRGALLPSMILSKEIQEYSSTGYLTPFALFKIKRDESKSENTMEYVLDL
                     DHSYFELSKLDGKHLLFADPMNATGGSLVTVVKYLLEQGVRPASIKFFNVISALKGTL
                     RVIRAIDNITVYTLWMDPSLNERAYIMPGLGDAGDRINGSDEDLHPRDMLRLVADYGT
                     NITGLYRSQLRIIEETVLRHRT"
     gene            17607..18512
                     /gene="nudC"
                     /locus_tag="PLS88_03075"
     CDS             17607..18512
                     /gene="nudC"
                     /locus_tag="PLS88_03075"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOU06362.1"
                     /translation="MTIFPESFVVFQGQDCLIPRQNADSMRFLDDPSLATSTKPEAVC
                     WPFYQIPPDLFSFTSDRYSYSAFEFDEKKYGAIQIEENDDIMLKRLGIGEAIPFPVRE
                     LVVSYPSGFSRFMLMAKAHSQWASISRFCGACGNLLVDAKENEEVKERLDDNAYAARF
                     CPSCKRIFFPRMSPAVIILVHRENSILLGHNVRFPRNRHSLIAGFVEIGETLEEAAIR
                     EIREEAGIEVKNLRYMRSQPWPFPDSLMIGFVAEWAYGEARPDRNEIDHIAWYRADDL
                     PELPLKGSIARYLIDSFIDGSFSKA"
     gene            18577..19992
                     /locus_tag="PLS88_03080"
     CDS             18577..19992
                     /locus_tag="PLS88_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HOU06363.1"
                     /translation="MNFSWKIVIDAGLISFALVIATFLRSKIRFLQKYLVPNALTAGF
                     LLLPLYNYFMPAIGYETNRLGDLVYHLLNLSFISMSLRSSPPKIKGSRSNGSVLGMSA
                     VILFGYASQALLGLVLTLFFLPNIHHAFGLNLPLGFALGPGQAYAIGKGWESMGFEGG
                     ATVGLTFAAIGYLWACFGGMLLIHKGIRLGWMKSEQVSIMSDKALLTGIIPNGTEKPV
                     GERLTTDSEAIDSFSFHFAVVVFVYLLSYLALRGISFLLGFAGKAGVELATNLWGINF
                     IFSAIIAIAVRKIMDLLKVGYVLDDDALSRISGTSVDYMVAGALAAISLVFVEKYWFP
                     ILLMSTLCGFMVYYTIPWVSSRIFRDFRFERMLMLYGVSTGTLSTGLALLRVMDPEFK
                     TKVTSDYMLSAGLTFVLAIPFILAINLPAKAYTSGSMVPYWIFMAIAAAYMLFVSVVY
                     WILARKRRFAAPRVNFYRPGK"
     gene            complement(19974..21254)
                     /locus_tag="PLS88_03085"
     CDS             complement(19974..21254)
                     /locus_tag="PLS88_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU06364.1"
                     /translation="MILNRRYSPTNFKFFTWLLRFTYGNWLKWKYRITAINTELFKEL
                     KPPFIMVGNHTTLLDPFIASTFVPFPIHWVASDGNMRNPIMRFMLIKLVGSIPKSKAI
                     PDIETVNWIVDLVKHKHSVVGMYPEGQSTWTGTPAPVFLSSAKLLRLLRVPVVLVKTN
                     GGYLAKPRWSHVRRQGKVEVSFSLLFSPEQLHKMELSTIDRLLNDALCYDDSAWCKQK
                     SLQFKTTKGAESLELALYICPSCGERASMHSSGNLFTCRQCGFTIEYQYDSSFLLRSK
                     GNRESYLSPFDEAPFLDSIVSWNSFQSRLLYRCLSGPERVNLEAPFFFDEPVKLKRGK
                     RMDRMETLIIGRIELYIDRLEFHDTRNAKLESIVFPIEQIDGEGVLKWNFFEFYNGMN
                     VYRVVFKDKKASGRKYSDAISVLHQISGSHFPGR"
     gene            21448..21915
                     /locus_tag="PLS88_03090"
     CDS             21448..21915
                     /locus_tag="PLS88_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOU06365.1"
                     /translation="MPCKKPLSSNNSYQKASNDSVSKTLERWLEIHEPVDKDKTKEDP
                     EADDRLDSISAYSRFKRMLPQDELDLHGLYAEEARQMIQTFIANSAARGLEKVSIIHG
                     KGNHSQEEQVLIHIVREELEKNPQAGEFDFADSRHGGKGATWVRIRPPISRDK"
     gene            complement(21896..22114)
                     /gene="infA"
                     /locus_tag="PLS88_03095"
     CDS             complement(21896..22114)
                     /gene="infA"
                     /locus_tag="PLS88_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOU06366.1"
                     /translation="MTKEEAIEVEGIVKEALPNTMFRVELANGHIILAHLSGKMRKHY
                     IRIVPGDKVKVGLSPYDLNRGRIIYREK"
     gene            22384..22740
                     /locus_tag="PLS88_03100"
     CDS             22384..22740
                     /locus_tag="PLS88_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOU06367.1"
                     /translation="MENAFIEKMKKLLLAQRQEILNTIAESNKELRKALSETDPKDSA
                     DIASDDIDRIMIEALSSQDLKRYQAIESALLRINQGRYGLCAKCGKKIPDERLEAIPY
                     AVLCVECQKSDERKNR"
     gene            complement(22742..23611)
                     /locus_tag="PLS88_03105"
     CDS             complement(22742..23611)
                     /locus_tag="PLS88_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOU06368.1"
                     /translation="MAFSFHTHTLFCDGKADAATMARTAFENQYTHLGFSAHAPISLE
                     TDCNLPWYKADSYVSAIRSLAQEYAPKGMKIFLGLETEYAPGIAMPNDPAYEPMHLDY
                     RIGSVHYITVPGEYPFTVDEPEEQFALHVQEWAPDGDYRKIWKRYWRHISEMIEVGGF
                     DIIGHFDLVKKNNIAGRWFDEENRAYVDAAFQALDLAAEKDYIAEINTGGITRSRRSE
                     PYPSVFILKRMQEKGIRITIGDDAHAPSHIGICQLKALEIAEIAGYKALWYMDAPGVW
                     KEAALASMKGQRR"
     gene            23741..25216
                     /locus_tag="PLS88_03110"
     CDS             23741..25216
                     /locus_tag="PLS88_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOU06369.1"
                     /translation="MSEFTARSVAIFFSVYSSGEKLARSLRQCCTHVAILSGYSEEIS
                     RKFDCIITETGLLNEREIHTILDSSTALVLIEKAVGKMPQAKNAEYSLEQFSNCALLR
                     VLPKNASNSTIKCAVYQLFAKMDSILSSQNTIAAAQSQLRTLLSSLPDIVYVLDEQGY
                     FLYLNESVGLLGYLPSELIGKHFGSIIHPEDRPNISRDLVVEKIRQNNIMPTNPPKLF
                     DERRSGSRMTRELEVRLLHKDGHVIYAMVNAYGEQAFDVPLFSDVVGKKLQTIGVIHD
                     ISAMHLYQESLEESLAAKERLLREIHHRIKSNLQLVASLAHLKQIDTKAAAAIEVLKD
                     LETQIKSIALVHEALYQSERVDRLSARDFFGQFCHFAEEALERIGSTVHIKVSAEDYP
                     LDPDLLVPLSLVLLYLLEGAYKASYERREKVEVYLSFVQKQNGMQIIEINGEGIFENA
                     DSIVMQALLKQAHANLEIPRGEDSGNFCRLIVETKSKMRGI"
     gene            25258..26199
                     /gene="trxB"
                     /locus_tag="PLS88_03115"
     CDS             25258..26199
                     /gene="trxB"
                     /locus_tag="PLS88_03115"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOU06370.1"
                     /translation="MGQIYDFAVIGAGAAGLSAAQYGARANLSTIVIEELAPGGQALF
                     IDKLENYPGILEPINGFELAERMRKQAEQFGSSFIEASALSVRKAEDRFIIETSVGPL
                     ESLTVLIATGARHKQLGIPGEQEYIGKGVSYCATCDGPFFKGKRILVVGGGDSACDEA
                     IYLSNLSDKILMIHRRDRFRAQKALASRVLSNPSIKVLFNTVAKEIKGNSKVHSVVLE
                     NTLSHELTEEPFDGIFIFIGSTPQTGFLKDIAIQFDESGYIITDCNMQTSITGLFAAG
                     DVRATPFRQIVTAAADGAIAARSAASYIDEQRGQAYR"
     gene            26217..26879
                     /locus_tag="PLS88_03120"
     CDS             26217..26879
                     /locus_tag="PLS88_03120"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOU06371.1"
                     /translation="MKKSMNFCTLDSVLRIPIDLVCGLDEAGRGPLAGPVTAAAVILP
                     KGFPITMLDDSKRMSARRREEAYCMIVEQALDWAVGWATVEEIEKYNILMASLRAMER
                     AFRGLSHLPALVVVDGLFAPSLSMNGTNIEAATMAKADGLIPAVMAASILAKVARDRM
                     MDCLDGIMPEYGFKNNKGYPTKEHRAAIERFGPSRFHRKGFHLLIQSDAPLFDSLESP
                     EE"
     gene            complement(26831..27235)
                     /locus_tag="PLS88_03125"
     CDS             complement(26831..27235)
                     /locus_tag="PLS88_03125"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOU06372.1"
                     /translation="MGIRGELFSTRFICEGRTYFFNVKENRNGDIFLSIVESKPTETE
                     TFDRRSIVIFQENMEGFMRAMRTAAGYMEKASQRPKPDRTAIQSSQPRQDPRQARQRL
                     QESSSQAPRRFVIRKKPHSSGDSRESKSGASD"
     gene            complement(27257..29233)
                     /locus_tag="PLS88_03130"
     CDS             complement(27257..29233)
                     /locus_tag="PLS88_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase"
                     /protein_id="HOU06373.1"
                     /translation="MPLISHPFLGLPIGDPGKIPLSREKLPTSKDVRLAAQELILSAS
                     GWRKIFAKPEIAAGYASWDYEQLPDNSLSPLLAPADIVIVAGMAIVFSSFIKEHCRPY
                     KPIVLLGIDTRRTSPTIADIFARVLIGEGCEVRYLFIVPIPEITAYAAQTTALDVDHE
                     FHSDGFVYISASHNPPGYNGVKFGLCGGVLKSEQIKPLISELKVFLSDPESSELALIA
                     LEKADARALTKCYENVAHWKRYSQSAYMLFAHSVFTDEETTEAQERILLEIANACEKR
                     PFGIIAELNGSARTQSIDLDWLSALGAESKALNMEPGIFAHRIVPENESLDDCIKALQ
                     SAHEKNAAYALGYVPDCDGDRGNLVYYNNRLREAIPLEAQQVFALACLSELAYLKWKG
                     ETNRIAIVVNDATSMRIEAIAEVFGAKVFRTETGEANVVCRAERLRLEGWTVRILGEG
                     SNGGNITHPSKVRDPLSTIGSMIRLLRLGDAQKNETCFNLWLEAIGSPERYQPDYDLE
                     DVIESLPQWITTSAFEPNAALKIRTIDKIELKNTYQRLFLEDWPKIQPELEQRFDIVS
                     WRAFASLGSDEWEIGSDFGASKNGGLRIVLYNKAGEPKAFLWMRASGTEPVFRILVDI
                     RGGTYADEAWLRTWHKGLVAKADLLASPRQNNVG"
     gene            29396..30316
                     /locus_tag="PLS88_03135"
     CDS             29396..30316
                     /locus_tag="PLS88_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU06374.1"
                     /translation="MFGIFKHFLFFIRGVKTYALVGPSGTGKSFRAKLVAQKYGIELI
                     IDDGLLIRGDQILAGRSAKKDPTYLGAVKTALFDDREHRDQVAKALQREKFRKILVIG
                     TSERMVQKICERLQLPHPVKVIKIEEIATKAEIEKAVQSRKVEGKHVIPVPALEVKRS
                     YPSIFYDSVRVFLRRSFGVGATLPKLYEKSVVRPEYAKRGRVAISEAALSQMVVHCVS
                     EFDPNVRIMRLAIRNDTQGYRITIMLEVPFGTRLPSKVHALQEYIVDSIEKFTGILVA
                     EVNIVIDRLTARPQKGQKWGQAIDGRVSSV"
     gene            complement(30342..>30776)
                     /locus_tag="PLS88_03140"
     CDS             complement(30342..>30776)
                     /locus_tag="PLS88_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06375.1"
                     /translation="RKFRASRSRVYDENDGLHVISPELGAVILSIPCVQGNARGNLQK
                     MKGRFKYRNGIYSVQIEIESLLEVFQIAVKAGNKRREIKRPPFCKGWPLFKNISAARS
                     FAGLMAIQSSFLFSLVRRYLLELSLSTTGHLFSPWKRLLQNH"
BASE COUNT         8664 a         6703 c         6838 g         8571 t
ORIGIN      
        1 ttatttacgg caatgggcaa gtgaattgaa gatcagcgat ttgttggaac gcgcggtaca
       61 tgaagcgcag taattggaga actccatgcc tgaacaattc ctttaccagc agctcgatac
      121 gctttcccag tggggtagta tcgaaaaaga agtccccgac agtgtagcgc gaaacctgaa
      181 ccccaaatac gaattgcgcc cataccaaat cgaagccttt gcgcgctttt tccattgtta
      241 tagaaacgac tttcccaaca agacttatcc tctgcatttg cttttcaaca tggcaacggg
      301 gagcggcaag acgctcatca tggcggggct tattctctat ctctacgagc agggctatcg
      361 aaatttcctg ttcttcgtca attccaccaa catcatcgaa aagactaaag ataacttcct
      421 taacccttca agcattaaat acctatttaa tccagatatt tttattgaca aacggcatat
      481 acacgtttca tcagttgaaa actttgaagc cgtaaacacc aatgatatca acatctgctt
      541 taccacgata cagaaacttc attctgatct gaccaacgaa aaagagaatt ctctgacttt
      601 tgaggacttt cgcaagcata agattgttct gattgccgat gaagcccatc atataaacgt
      661 caagacgaaa tcagagggag aattattcga aagctgggaa aataccgtcg aacgcatctt
      721 tacgcagaat gaagacaact tgcttctgga attcaccgca acccacgatt acgccacgcc
      781 aacgatggtg aagaaatatc gcaataaagt gattattaaa tatgacttat tacaattccg
      841 caacgatcgt ttttcaaagg acgtggttat agtacaatca gattttgatc aacaggagcg
      901 cattctgcaa gctcttatct tgagtcaata taagcaggaa gtggcggcaa aatatcgtat
      961 taatctaaaa ccagttattt tgtttaaagc ccagcgaact atagagcaat ccaaaaaagt
     1021 ccaggcggaa tttcataaac tagttgatga gcttactgcc aggcagattg ctggcattcg
     1081 caagtcaaac attccccttg tgcaacgtgc ctttcgattt tttgatgaac aaagaataag
     1141 cgatgagcaa cttgcgaatc ggttaaagca agaattccaa cctgattttt gtttatcggt
     1201 aaacgatgag aaagaaaaag agaattatca aattctcgta aatacactgg aagataagaa
     1261 taaccgtatc cgcgccattt tcgccgtcca aaaacttaac gaaggttggg acgtgctcaa
     1321 ccttttcgat attgttcgat gctatgaaac gcgtgattcc ggcaaaggca aaattggcac
     1381 taccacatta tccgaagcgc aactcatcgg acggggagcc cgctactttc cctttgttct
     1441 gccgggaagc gatgaccgtt ttcgccgtaa atttgacggc gacctggggc atgaactgcg
     1501 cgtgctggaa gaactgcact accacagcat caacgattca cgatatatct cagaaatccg
     1561 tcaggcgcta atcgaacagg gtatgatgga cgagcgcgaa gtaactcgcg agctaaaact
     1621 gaaagagtca ttcaaggaaa caccattcta caagtatggg attgtctggc tcaacgatcg
     1681 tcagcgcaaa gattaccaac ggatttactc ctttgctgat ttaggagtga agaagcgaaa
     1741 ctatatccac tgtatcgcta caggtcaggg cggtacaacc gccgctttga agaacgcgga
     1801 aaaagagttg atgtggaaag acgaagcctg tcaggatgtg aaggtgcggg acatcgagcg
     1861 caacattgtt caatctgcta ttgcacgtaa tccgttcttt acctttgcca atctcaaaca
     1921 ttattttccc catcttgtct ctatgcacga cttcatagct tcttatgatt atctcggcgg
     1981 attggaaatc acttttcagg gtaatgtgta cggcctaggc tacaatcgcg ctgaacaact
     2041 cgccgccatg tcaggcttgc tgaatcagat cgaatcagaa attcgcgcta atgtcaccga
     2101 ttacgaaggc acgcacgaat tcaggcatga ttgggtacat gaagtcttta aagacaagat
     2161 actgaaattc aatgctcaaa atccccgcgc cgccgacgac gcacagtttg agtatttcgt
     2221 ctctaccaaa gactggtttg ccttcaacac catttatggc acaagtgaag aaaaagcttt
     2281 tatccgtatg tttgataggc aaatggaaaa attgcaagaa caatacgagc aaatctatct
     2341 attacgcaat gaaggccatt tcgctatcta caactatgct gatggtcaag cctttcaacc
     2401 agactttgta ttgttcctgc gcgagaaagg cggcaagttg ctcatttacc aactattcat
     2461 cgagcaaaaa gggagacaca tcgaagagta tgaccgctgg aaagagattt ttctagaaga
     2521 aatcacttcc gagtttggca gtaaatcgct gacatttgaa ggcaagaaat accgcttgat
     2581 tggcttgccg ttctacaaaa aagaagacga aaaccaattc atagcaaggt ttgaatcagt
     2641 gttgaattag cataatcagc aaataaagac cgttcctcaa tgaggaaaag attggttatc
     2701 ctatattccg aaagaaaacc aggataatta acttttctct atcacaggaa tgatacaggt
     2761 ttgatagata ccaacaaggt gagagcagct agccctggag gccagctaga gccttctccg
     2821 acaccgccat ctctttcata gtaatttttg attcttttta gcccctaggg gaatcgaacc
     2881 cctctttaaa gattgagaat cttttgtcct gaccgataga cgaaggggcc tcggccacat
     2941 ctcggcgaga tgctgacttg agctgaagct caggctctcg caccagcgca aaaataaatt
     3001 ccccaggagg gattcgaacc cccacaagca gatccagagt ctgctgtgct accattacac
     3061 aaccggggaa tgacgacctg taccttagtg atttttatag ttttggtcaa gagtttctgt
     3121 actataagcc tttcaaatca gcgcgatgga tatcatacaa ctcgagttga ggcctctctt
     3181 cgccgttcca ataatttgta gtgactttgt atataatatc gattctatcg ttgtttctga
     3241 atgaaaaatc tcgttcgagc ctttgtgctc catcccagag catggcaggc catttgtatg
     3301 tgccgaagtc cagcgtcagc ttgagatggc tcttgccatt tttcccgact atctgagcat
     3361 caaccattga gacattcctc gaatagaaga caagaggttt gctctcttct ccgattggct
     3421 cgaattgacg gcatagctgt agcaaatctg gagtaacaaa agaatgtggg agttcggcat
     3481 ctatcgaaag ctccgattct tccgttatga gctctgtatg cgccatgaat tgagcaatcc
     3541 gttcaagaaa agcctgccaa tcgtcggttt tcatggtaaa accagcggcg gagtcatggc
     3601 ctccataatc aaggaagaga tcagaggaaa aggccaagag ctgggtaaga ggaaaggtat
     3661 ccccactacg aatggaacct gtacaggttc catctgtttt gaatacggcg acaatcacag
     3721 gtattttcat gagattggcc gcacgtgagg caagtagtcc cgtaattcca ctttttatct
     3781 tggaactgcc cactacggca aatttccctc cgctcttttt gaggctctct gcgagctgat
     3841 cttgaattac ttcccaagct tccgctccaa ggcgcctgcg ctcgttgttt gcttgtatta
     3901 gctgtgaagc ggcctcgata gcttcatttg ttgtttcggc ttgcagaaga tctagagcta
     3961 gttttggctt gccaagcctg ccagcagcat tgataatggg tgtgatctgc caagctacat
     4021 catttgatcc gagcggtctt ccaagattga gtactatctt gagctctcgg attccattag
     4081 tcggagcagt gtttagactt tctattcctt tccgcacaat cagtctgttt tcattcttta
     4141 gaggcatgag atctgcgatt gtgccaagcg cagcgagttg cagcattcta tcgctcagga
     4201 ggctctggct ctgaagagta tagagtaagt acccattgaa aatatgtttg agggtatcga
     4261 tagtgctata aggaccatag gcatactttt tcagtctaga aatctgaaca agttcttcca
     4321 gggaatttgc cgacatattg ggccatatag gtgtaactac tttctcgagg tctagtacct
     4381 cgagttcgac taggccagca aaatttttct tgaagaaggc ttctattgtg tctttattcc
     4441 atgcaactac aatacggcca cgaagaaatt tttctagctt ttcgagagtt cccgagggga
     4501 aatatcccct actgtcagaa ataattctta aacggcttgt ctcaataagg ttatcgagcc
     4561 tgatcgcttc tatctcgaga ttgccttctt tctcagtatt gagcttgttt ttttctatat
     4621 ttaggcagat ttcttggtga atagcttgac cagcgcgaag aagggcgagt ggttctttga
     4681 gaatgcctaa acgagctatg gagagggcat gcgcaagctt tagggccaca ccgcatccag
     4741 atagatctct aaaaggatat ccacagtccg gaatcttggg atttattata gctatggctt
     4801 ttggtggctc ttctgcttgt atgtgatgat gatctgtgat tatgacatcg attccttttt
     4861 ggctcgcata gagcacttcc tcatgattcg aaataccaca gtctactgta acaataagac
     4921 tcacatcttg ttcttccgca aagtctatcg aagcctttga tagaccatag ggttcctcgc
     4981 cttctggaac cttttgaagt actttcagtc caaaatctct aagggcttcg gtcatgagca
     5041 ctgtcgaggt aataccatct gcatcagaat caccaaacac aagtactttt tcttcttctt
     5101 ctcgggctgt gataattcgg tctaccgcat cctccatgtc tttgaagagg aaaggattat
     5161 gtagcagttg tagatcgctc tcgagataga actgtatttg ttctggctcg attatatttc
     5221 tgcgtaccag aatagaggcc gtcagagtat ctatttcgta gcgccgagca aggctgcgca
     5281 cttgaactgg gtctatctct ctttttatcc acttcatgat acggtaattt cttcaagaat
     5341 aagtgatgat attgatggag cggtgttttc tatggaaatg gcattcagag cgagagggaa
     5401 tgcagcttct aggctttggc tgtctttgcc aaatagcgtt gcgatgcgtt cgccctttga
     5461 tacatggtac ccactcttct tatcgaagat aaagcccgct gtggggctta cgctgtcttc
     5521 tgttcgattt cttcctactc cgaggtatac gcctgcgagc ccgattttga gagcatcgat
     5581 ggaagcgata tatccatctt tatcagctct aatttccata gagtattctg agcgccagga
     5641 tcctcttttt ttttgcatct cttctagatt gccaccctgt cgtactacat ttcggtagaa
     5701 aaggttgagt gcggccttag actctatggc ttcttcgcat aggcttttgc cttccgaaac
     5761 atcctttgct ttaccaccta gaacaagcat ccaagctccc agccaatatg taagctccat
     5821 aacgtcttta ggtcccttac cttcgagaca atccagagcc tcttcgatct cgagaaaatt
     5881 gcccactttg tatccaagtg gttgcgacat attggttagt actgcaacaa ctttttttcc
     5941 catttttttg cctgttgcaa caaggctttt tgcaagagcc ttggcatcat catagccttt
     6001 catgaatgcg cccgagccac attttacatc gaagactata ccttctgcac cttctgcaac
     6061 tttctttgat aatatcgaag ctgttataag tgggatggac tcgattgttc cggttacatc
     6121 cctaagtgca tatagctttt tatcggctgg aacgatatgc gcagtttgac ctgtcatcgc
     6181 gaagccttct tccagtaggc cttttcgaaa ttctgcaggc tcaagatcag ttatatatcc
     6241 agggatggat tcgagcttgt cgagagttcc tcctgtatgt ccaagagctc tgcctgacat
     6301 cataggcact cttactccac atgctgcaac tattggagca agcggtagcg atatcttatc
     6361 gccaactcca cctgtcgaat gcttatctat aaaaggccct gggattccgg aaagatccat
     6421 tctttccccc gaatcgagca tagcttgggt aagggcagcg gtctcctgcg ctgacatgcc
     6481 ttgaaagaat atcgccatca atagagcaga aacttgataa tctggtatct cgccctttac
     6541 atatccttcg ataaaaaagc ggatctcttt ttcatcgagg gattcgccat gacgtttctt
     6601 aataatgata tctacagctc tcattaagat gttctccaga atctgcttta ttaatatgcg
     6661 aatattgaaa actattttac ttcaaattta agttccataa aaggtctcac caaaaacccg
     6721 aagactctgc agagggcctt ccgcggcaga ttcggcaagt gattcgatta ttgcaattat
     6781 ctcaggacgc aaagacgcag atttcaatag aggcaaggcc tcgctgtagg atagctttgt
     6841 aatgctacga agacatgtaa gaaccttctc atcaaaagga agcatcgccg aggttgaaag
     6901 tcccatctgt ctgaattctt ccgcacagtt atgacaaaga aaggagcttt catttatcaa
     6961 aagttttcct ggcagggaat tcttctcaga aaaagatcta ccacatctct cgcagcttaa
     7021 aagatcagga attaagccaa gtggggaaag agcattccag aaaaaggccg ataagactaa
     7081 tagtgcttct gattcatcta cattggcgag ctcacgaagg agtgcggtaa tcatagatag
     7141 agcttgtggg tattcgccgc cgaaggctga agttcgtatc aagaattcag atgctgcgca
     7201 tgccgattgc atatgcgaaa agctcttatt aataccatcg aagacttcaa aaacattcgc
     7261 acctgttagt tttataaatt cccgccgtgt atcatgatat acttcaacat cagcgactat
     7321 gaaaggcaaa gcggctgaac ggagagagct ttttggccct ccgaacataa aaagcgatag
     7381 tagcccttca ttgcatagaa aggttagaat cctatcgccc cgggccgtgt cctttgcatg
     7441 gagaaaaagg cctctgtatc gttcatttct ctgagccatc gatcaatcat cgtccacaat
     7501 ccaccaatgg aagccatcgg agtagccagc agtacggctc ttgcgccatt ctgcaatgcg
     7561 ctttaattcc ttctttttat aggacttacc ccatagataa gcgctcaaga atcctcctat
     7621 atgtgcaaaa taggctgtgc ctccattctg aatggctgta aggccgctca atccgcctaa
     7681 gatttgcatt aagaaccatg ttacaataac gaaccaggca gaaaatgtgg tcggtatgaa
     7741 ataaaacaga agcacgacaa ctctattgcc gggaaacaat accagatatg tagcgagcac
     7801 tcctgagatt gcggcagagg caccgacgag gggcatttgt agcgcagcgc ctcctgcaag
     7861 agctgctagt aatatctgca acagcattcc tatagttcca gagatcaaat aaaggagaat
     7921 atatcggagc ttacctattc tacattcgac attatcgccg aatattccaa gaaacatcat
     7981 attcccaatc aaatgtgcaa accccgcatg ggtgaactga cttgttatta aagtgaacaa
     8041 tcgtcttccc gacaggatct cctctggaat tgctgcaagc gctagagtta cataatcgtc
     8101 taagccaaat ttctgtatga aaatgaaaac caatatattg agagcaatga gaatccagtt
     8161 tatgacagca ttgccgcgac ggcaagtatt atcatctcca attggaatca tagaagctcc
     8221 tcattaaaag cactatatct agaatagcat attatctgct gataggctag ttttttgctt
     8281 cgcttgtcgc aaatatacaa ttcatgatac aaaagaaaca catgattagc gccttgctct
     8341 tcgatctcga taacactctg tattccgaag ctacgggctt ggaaaacgga gtctcacaga
     8401 ggataaatca ttttattgcc gattttttta gactctctat tcctgaagcg atcaggttta
     8461 ggcgtgagca cgcaaaagca tatggcacta ctctagagtg gctcatgcga gactatggat
     8521 ttcaagagcc agaaaaatac tttaggacta ttcatccgga ggatgaagca gattgtctca
     8581 atccagatat ggttctaaag ctaatgttga acaagatacc tataccaaag gctgtcttga
     8641 ccaatgctcc catggagcat gcgatcagaa ttctggataa gttgggtatc atggaatgtt
     8701 tctctggtat ttacgatatc tggttcaaca atcttattgg caaacccaat tcagactcct
     8761 acataaatgt tttagaagct gcaggattcg atcgagaaca aacacttttt attgatgatc
     8821 ttccaaagta tgtaaaaggc tatgtcgata tcggaggacc agcagttcta aaagatgaaa
     8881 tggatcgttt tgcagatctt ccttttaggc gcctaaaaac gatttacgag ctcgacaaag
     8941 ttctagacga attcaattct tcttatatgt aaaactttgc tattattctc tttcagccaa
     9001 gataagcgaa ataagagtga ctagataagg taaggcaagg acaagcaagg gcggtattga
     9061 agagaatccc tggatataat tagaaaggct ctcggcaaaa gcgaaaagga gacaggatgc
     9121 agctatccaa agaggctttt ttctaccaag gtatatagcc accagtgcga tccagccacg
     9181 ccctgagata atgtttggca caaatgcaga aatagacaag cccaaagcat atccgcctaa
     9241 tccgctaccc accccagacc agaggaaagc ctgcatatgt atccgatctg gagaaaagcc
     9301 aagagctgaa accgcctgag ggttggagcc tgttgcacga atttttaagc catgcttagt
     9361 attttttatt atgaaagccg aagtcaaaac ccatagccat gcaaatatag tgatcatcga
     9421 gtgagaaaag aaaatggggc ctattaaagg aagtcgcgat ataaatcttg ggaatagatg
     9481 attatcgacc gagatgttga acgccacgac cgcttttgtg tggtaccagg attgagacaa
     9541 aactgccgtt atgccctgag caagcagatt gattgctagg cctgtaataa atagatttgt
     9601 tcgaaacttg ttgatcatta agcccatcaa cattgaagca agcgaggctg agaggatcgc
     9661 tgcaagagct ccaaaaaaga tattacttgt caaaccagca gctgccatgc caaaaaacgc
     9721 accgatagcc ataagtcctt ctagtgctat gttgagcatg ccggaaagct ctgaaaaaag
     9781 gcctccaatc gaagcgaaaa gcaggggtgt tgccgcggct attgtatatt tcaaaaagtc
     9841 tattaggact ataggtgaga agttcattat tgtctttgcc tctgccacaa tttaggcatt
     9901 acctgcgctg taataaagag catcgcagtt gcctggagca tagaaataat ctccgaagga
     9961 actcccgatc caatcatgac atggtcggaa cctgctttta gaaatgcaat aaaaagagca
    10021 gctggcaata tggcagtttc tctgccctgc gcaaggagag caactgagat tgcactccag
    10081 ccaagacctg aagaaaaacc ccgcatgacc cgaccttgtg gcccgatcac aagaagagcg
    10141 cccgatagtc catacatgcc gccagagatg gccatagctg cgatattgta agctcgaacg
    10201 gaaatgccac aatatcgtgc aaattcctcg ttttctccgc aaaccctcaa ttcgaaaccc
    10261 atcttggatt ttttggataa gatatgaaaa aacaagacga gaaagagggc aagccatata
    10321 gagatatcta gtttagaagg agaaaagatt cttggcagaa ggaaaacaga aggaatctgg
    10381 gccgtggttt gaaaattgct tgtgctgtct tgaagcgggc cggtgatgaa gaaatcgctt
    10441 atatttataa tgattgctga gataagatag gacgatatca tctcggaaat ccgccaatat
    10501 gcttttagaa gcccagatat tgtaccgata atggctccta taaaggcccc gataaaagcg
    10561 accagaatcc acactgtgcc tggaccgaat gcttcaggag catgcagcgc tagaactccc
    10621 cctgcgagag ctcctgcata aatctggccc tcacctccta gattaaaatt cttagatcga
    10681 aaggcaatag agacaccaat gccactaatt ataagaggca cagtatcggc aatgtagttt
    10741 ccaaaaaaat atcggctcga gaagggctct acaaaaaact gccataacgc agatctaaag
    10801 tcctcagcaa acaataaaag cacggttgcc gcaagaataa tagaaaaagc agctgccata
    10861 agaactctaa acgtagccga aaatgccctt tgatttctca tccatgaatc ctgttaccaa
    10921 gaaggtattc accgataatg gctctattga gtagactgct atttttttga tacaaaactc
    10981 gtctgcctcg agagagtacc atgatatgat ctgacaattc gagtatttcg tcgatattcg
    11041 aggatagaag aagaatcgcc gtcccctctt cttttgctag gcgaattctt ctgcaagtct
    11101 gttcagccga acgaatatct agactccagg tcggttcgca tagaataaaa agtttaggga
    11161 agtctcgaga aaaaaggcga gcaatggcca atttttgaat attgccacca gagagctcac
    11221 ctgcaggttg atcggggagg gcatttatct cgaaggaacg aatcgcatct aaaattgctt
    11281 gttgtgcagc attttctaga atccagcctt tggggaaaaa gtcatgtctc gaaagagcta
    11341 tgaaattatc tcgtacggaa agagaagagg cgagtcctct ttttatgcga tccgatggta
    11401 ggtaggcaat aagtcccttt tgaaacaaac tttgatatct tcgataatta tggggctcta
    11461 tatgatgacc atcgaagata aaagttccaa aagatggtga ttgaaatgca gcaagtgcat
    11521 tttcgagatc atctagacca ttgccgctca atgcacaaat gcctaggatt tcgccggcat
    11581 gtacctcgaa ggatatatct gaaaggccaa ttccataata tcgaggacga cgaacggtca
    11641 ttccatcgac acccaatact aaaggatttt tttgagacct ctctatttta gacttatcta
    11701 tttgagactt ctctataggt tcttgttttt cttcgattga accttctccc atgataagcg
    11761 atgtaagttt ttgaagggtg gtcgcagata tatcataggt tcctattgtc cttccctgac
    11821 gcattatggt taccctatca gctatctgca ttacctcatg gattttgtga gtaacgagga
    11881 taatagtgtg acccaagcgc cttatatcgg atagtttttc aaagagtacc cttgtctctt
    11941 gttctgtgag tacagaactg ggttcatcga gaattaagat ctttgcatgg cgcgcaagtt
    12001 ggcgcaatat ttcgatctgc tgacgagcac cgatgctcaa ggaagcagca ggcgccgatg
    12061 gatcgatggc aaatccatat tctgtagcaa gctgctgagc tctggtattt agctcctgcg
    12121 agtcaacaat gcctgctaat ctggaaatct tattgtttcg aataggctct atgccaaaga
    12181 aaatgttctc tgcagcactc aactcatcga atatcaggaa atgctgttga accatgccaa
    12241 ttccaagggc ctgagccttg ctaggcgatt cgatttttac aagccttcct tcgatttcaa
    12301 tgcttccttc atcaggaaga tcaaggccag ctaatatctt cattagagta gttttacctg
    12361 ctccattttc tccaacgatg gcatgaatac tgcctttttc gacctcgagc gaagcaccat
    12421 catttgcgtg gactcctcta gggtaatagc gtctgcaaat tccctgcatc cttaccgcga
    12481 gcatagggag catattacaa tgaaggaacc gcgaaggtaa gttgacccga tttgatcgat
    12541 tggattactg catcgacctt tatacgaata gattctggta catttttgat ataagaagga
    12601 ttcttgttca agaattctat gtatccctct ttcataccca ctatttcggc ctttccaaag
    12661 ggcagtttcc cttcgattgc tgctttcaaa ctgcggtagg cgagttcttc ctgatgcaga
    12721 atagtgcatc caagaatatt gtcgggagca cgtgcaaatt catcgtcatc gaagaatacg
    12781 agatactttc cggtctcttg agcaactctg acagcgcctt ggcttgctga tccacagatt
    12841 ggcaatatga tgtctgcgcc ctgagcatat agactacgac tcaattctgc agctttagca
    12901 gcatcgaacc agtttccgag cacacggatg tcgagctgaa aagcaggatc aatggcccta
    12961 agccctttta gaataccagg aattatcatt ttttccattg ccggataatt ctgacctatg
    13021 atcatgccga tttttttttc cgggtttgtg cctttcatgc cagaggtaga gacaagccct
    13081 gaaagatatc ctgtgatata gccttgctcg agttggttgt atagtgcgga atatacattg
    13141 gaattaccta taagataacc atctaggcag ataaacttct gttttgggaa gagcttggaa
    13201 gcttcattca ccagctctgg catcgatgga ttggaggtga ggacaatgtc aaattttcca
    13261 gaagcgacga aggagatgag tttttcttgc cattcggctt ggttgaaacc cgcctctatg
    13321 acttttatac ttgcacctgg tgtttcggag acgaggcgtt ccgctccttt tgccatatta
    13381 tcgtagatag ggcttccgtc gcgtgcgccc ggaataaaaa cgcctatccg gggggcttgt
    13441 gcccatagtg cagtactcgc cagaataacg actagagatg cgatcataaa tcgacgcatc
    13501 ggattcctcc tgctataccc tagagggcag gaaatatgat agaatcttac atagcttaaa
    13561 atctaacgtg agaaaatatc tcacgttaga tttagtatag cgatcttcat aatatgttca
    13621 aggggtgtac acattggccg aaaagcttct aaagatcggt gaacttgctt cactttttgg
    13681 ggttacggcg cgaactattc gctactatga agaacttggc ctcatagaag caagcaatcg
    13741 cactgaaggc ctccatagac gctacccagc tgatgccatc gtacgattaa agaggattga
    13801 agaacttaaa tcgctggatc tcactctcgg ccaaatccgt gaattcttca tcttatttag
    13861 cgaagatcca agtggtgaaa aatgccgact tcttcttata cagagctacg aggaccaaaa
    13921 aaaggaagaa gagactagaa tcaaagaagc acagaatcgc attgcccaaa tcgaagcaaa
    13981 cattataacg ataaaagaaa aaaagtcgtt cttttcctgc cctggtgagg aatgcaaagt
    14041 ctgcaatttc aatggattct gtgtggattc agagcataat tctgatcagg aaatgaaagg
    14101 atgaaaaaac taggctttgt tgttccatgt attctgagag ctattcagag tatatcgtca
    14161 tgccgacaaa ctaactgcct ttgctttgca agctctgcaa aagcataaag tgccttgctt
    14221 attcatgata ttgcatagga gttgaatatt gattcttgct attgatatcg gtaccactcg
    14281 aatcaaggcg gccctcttcg actcagaagg gaatagcctc aaattggaat tattagatct
    14341 tccagatgaa ggttctaatt cctaccaaga aatagatgca cgcgtttggc ttaaggcatt
    14401 ctcagagatt atctctcgtt tgatatcggc cggagaacag aatgatctcc gagctattgt
    14461 tatatgtggt aatgggccca ctattcttcc tatcgatgaa aaaggcgagc caattgcccc
    14521 agcaatcaca tggctcgata ggcgcgctca aaaagaagcg aaagaggctt ctattgcttt
    14581 aaattactct ctcgatgctg ccttcaatct tcctaagatc ctatggatga aacggcagac
    14641 accgaatata tacgagaaag ctaatctctt tgtatcctgc ccagaattca tcgcaggacg
    14701 tcttaccgga gaatggacaa cctgtctacc aaatcagggc tataagagga ttatatggga
    14761 tagaagagcc cttgaaatgc ttgagctaga tgtagaaaaa tttcctcgct ttattggtgt
    14821 tggtgagata gtaggacata ttcatgatta tgccgcaaag gaatttggtc ttcctaaggg
    14881 cttgccagtt gttatggcgg gaccggattt catagcctcg ctcttgggta cggcaacagt
    14941 taagccaggg aggacctgcg acaaagctgg gacttccgaa ggcatcaatc tttgttcaga
    15001 aagagatatc ggagatgtag aagcgcttct tgttatgcct catatcattg ttccgtattt
    15061 caatatttcg ggagtaatat catcgagtgg catagccctt tcgtggttca gaagcagatt
    15121 cttgcccaaa gaagatttcg agacgattta ttcgcgagta gcctcgacga aacccggagc
    15181 agagggactt gtatttcttc catatcttgc tggtgaacga tcgcctcact gggaccctaa
    15241 tgcgcgagcc gtctttctag ggctagggct tcatcacgat ctcaatatga tggcaagagc
    15301 ggtactcgaa ggcacagcct ttgctattcg agaagttctg gatgtaatgg aaagctcagg
    15361 agcctctgtg aaagatatga gagctacagg cactccctcg ctctctcccg tatggaatca
    15421 gatcaaggca gatattacta gaaagcggct tatggtatca tccttcccag aacccgagct
    15481 tgcaggttgc ctcgctattg gtcgatttgc tttaggtgaa gagtctaccc taactgaagc
    15541 cgcagaaagg gtattcatac cagccgagat cttcgaacct cggaatgaca cagaatccat
    15601 gtactgtgac ctatatgaag tctatataga tagttatcgg cgccttgcag atctttttcc
    15661 tgctatttca gggcttcaaa agagtgcaat tgttcaacag gggccttttg gcaagggttt
    15721 ttgacaaagg cttttcaagg aaaattaagc ttgaataaag ataataagcc acattcctat
    15781 gctgacaata atcttcctat tcttgcgaca aagtctcttc tgagatagga tattctatcg
    15841 tgcttatgaa aaaacaatat catagccgca ttcagtctat tgttcttagc caggaggatt
    15901 aaatgcccaa gatggatata atcatcaatt tatcgaacca ccatatccat gtaaatcatg
    15961 aagatctcga gatacttttt ggaaaaggcc atgagctcac taagactaag gaccttattc
    16021 agccgggtca gtttgcttgt gcagagaccg taacggtaaa ggggccaaag gggcagctcg
    16081 aaggagtgcg aattcttggc cccgaacgca aagaaactca gtgcgaaatc cttatgagcg
    16141 atgttttcaa gttaggcgtg caaggatgcc cggtatgcga atcaggtcag cttgaaggta
    16201 gcttcccctt tgaggtgatt ggtcctgctg gctcgataaa aaaggaacga ggtcttatca
    16261 ttgcaaagcg gcatattcat ttcgacatag aaacagccaa gcattttgga gtcagtgaca
    16321 agcagatcgt aaaacttaaa gcaggggaag aacgtggagc cattcttcta aatgtggtat
    16381 gcagagtcca ccctacttat gcgctagaat gccacatcga tttcgacgag ggtaatgcac
    16441 ttggcattac aagcggagcg ataggcgaaa tcgtacagtg ttgatgtgtc gactagatca
    16501 agcgcaatgg gagtgtgagc ctatagattg gagcaccatg tgaataaatt tattttgaaa
    16561 gctacggatc tagatggtta cctcatggaa agcgatctcg aatacctttc tcggatgaat
    16621 gagctctatg agcaagcaat ggcctctttt aagattcttt ccaccagtac gtcggaagct
    16681 cagaaacgcc atgaggaaga tattttgata gagctttaca atcagatggg caagctcatg
    16741 caagagatct gcgcatcgga gccgcgcatt caagtctatt cctttgtgtc tcctcaggag
    16801 acacacggtg aagtttcacg attgattgcc aagttgcgag atgtgaatac cgggcatcag
    16861 gagttcgttt actacattca gcgagctttc gagcttttgt ttacactggc ttttggcgta
    16921 gcaggcaaga caaataagaa ctaccttatc gtgcgcacgc ccgtcaatat tcctgtacag
    16981 aactatgctg tgcataaaat acccaatgta gatagcgctg tgcacgatac ggtcatgtgt
    17041 gtcatgttga ggggtgctct cttgccatcg atgatactct ccaaggaaat tcaggagtat
    17101 tcttcaactg gctatcttac tccttttgcg cttttcaaga tcaagcgcga tgagagcaag
    17161 agcgaaaaca ctatggaata tgtgctagat ctcgaccatt cgtattttga gctttctaag
    17221 cttgatggca aacatctgct ctttgcagat cccatgaacg caactggcgg ttctcttgtc
    17281 accgttgtga aatatcttct tgagcaggga gtgagaccgg cttctataaa gtttttcaat
    17341 gtaataagcg cgctgaaagg gactctccgt gtgattcggg ctatcgataa cataacagtt
    17401 tatactctat ggatggatcc aagcctcaac gaacgtgctt atatcatgcc agggctgggc
    17461 gatgccggtg atcgcataaa cggcagcgat gaggatctgc atccgcggga tatgctaaga
    17521 ttagtcgcag attatggaac caacatcaca ggattatatc gttctcaact tcggataatc
    17581 gaagagacgg tacttaggca cagaacatga caatatttcc agaaagcttt gtcgtttttc
    17641 aaggacagga ctgcctaatt cctcggcaaa acgccgatag tatgcgcttt ctcgatgatc
    17701 cttctctagc aacatcgaca aaacctgaag ccgtatgttg gccattttat cagataccac
    17761 ctgatctatt ctcttttaca tcggatcgct attcctattc agcatttgaa ttcgatgaaa
    17821 agaaatatgg agcgatccag attgaagaaa atgacgatat catgctcaag cgattaggta
    17881 ttggcgaagc tattccattt cctgtccgtg aacttgtcgt ctcttatcct tcagggttta
    17941 gtcgattcat gctcatggcc aaagcacata gccaatgggc atcgatctcg cgattctgtg
    18001 gagcatgcgg gaatcttctt gtcgatgcaa aagaaaatga agaggttaaa gagcggctcg
    18061 acgataatgc atatgctgca agattctgtc cgagttgtaa gagaatcttc tttcctcgaa
    18121 tgtcgcctgc ggtcatcata cttgtgcata gagaaaattc gattctatta gggcataatg
    18181 tacgatttcc ccgtaatagg cacagcttaa ttgcaggctt tgtggaaatt ggagagacgc
    18241 tcgaagaagc agccattcga gagatacgcg aagaagcagg gatagaagtg aaaaatctac
    18301 gctatatgcg ttctcaacca tggccattcc cagattctct tatgattgga ttcgttgcag
    18361 aatgggcata tggcgaagca cggccggaca ggaatgaaat cgatcatatc gcctggtata
    18421 gggccgatga tctgccagag cttccattga aaggaagtat tgctcgttat ttaatagaca
    18481 gcttcataga cggaagtttt tcgaaggcct gatctcttta gtatttgatt ttgcgcgaaa
    18541 ttcatcctac acttgtcgaa tatataggag caaattatga attttagctg gaaaatcgtt
    18601 atcgatgcag gactcatttc ctttgctcta gtgattgcta ctttcttgag gtctaagatc
    18661 cgttttcttc agaagtatct cgtgccaaat gctttgacgg ctggttttct gcttttgccc
    18721 ttgtataact attttatgcc agccatcggt tatgagacga acagacttgg agatcttgtc
    18781 tatcatttgc taaacctctc ttttataagc atgtctcttc gttcttcgcc gcctaagatc
    18841 aaaggttctc gttccaatgg aagcgtgctt ggcatgtcag cagtcattct ttttggatat
    18901 gcttcacaag cgcttcttgg ccttgttctc accttattct tcttgcctaa tattcaccat
    18961 gcatttggat tgaatcttcc cttgggtttt gcccttggcc ctggacaggc ttacgccata
    19021 ggaaagggtt gggagtccat gggttttgag ggaggagcaa ccgttggact tacctttgcg
    19081 gcaatcggct atctctgggc atgttttgga ggaatgcttc taatccacaa aggcatacgt
    19141 ttgggttgga tgaagtcaga gcaagttagt atcatgtctg ataaggcttt gttaactggt
    19201 ataattccaa atggcactga aaaaccagtg ggagagcggc taaccacaga ttctgaagct
    19261 atcgattcat ttagttttca ttttgcagtc gtagtgttcg tatacttgct cagctatcta
    19321 gccttaagag ggatatcctt cctgcttgga ttcgcaggaa aagctggagt agagcttgca
    19381 accaatcttt ggggtattaa ttttatcttt tcagcaatca tagctatagc agtcagaaaa
    19441 atcatggatt tgctcaaagt gggatatgtg ctcgatgacg atgccctatc tagaatttct
    19501 ggtacttcag tagactatat ggttgctggt gctcttgctg ctatttcgct ggtatttgta
    19561 gagaaatatt ggtttccgat tctccttatg tccacattgt gcggattcat ggtgtactac
    19621 acgattccat gggtgagttc gcggattttc cgagatttta gattcgagcg aatgcttatg
    19681 ctctatgggg tctctactgg cacgctttct accggtttgg ctcttctaag ggtaatggat
    19741 cccgaattca agacaaaggt aacctcagac tatatgcttt cggctggcct taccttcgtg
    19801 ctagctatac catttattct agctatcaat ttgccagcaa aagcctatac gagcggatct
    19861 atggttccat attggatttt tatggcaata gctgcagcct atatgctctt tgtgtcagtt
    19921 gtgtattgga ttctcgcgag aaaaaggcga tttgccgcgc ctagggtcaa tttttatcgc
    19981 ccaggaaaat gagacccaga gatttgatgt aacacactga tagcgtcaga atattttctg
    20041 ccagatgctt tcttgtcttt gaagactacg cgatagacat tcatgccatt atagaactcg
    20101 aaaaaattcc acttgagtac gccctctccg tcgatttgct ctataggaaa aacaattgat
    20161 tcgagcttgg catttcgagt gtcatggaat tcgagacggt ctatatagag ttcgatacgc
    20221 cctatgatca aagtctccat acgatccatg cgttttccgc gctttagctt gaccggctcg
    20281 tcaaaaaaga atggagcttc aaggtttact ctctccgggc ctgagaggca tcgatataga
    20341 agcctactct ggaaactatt ccagcttacg attgagtcga gaaaaggggc ctcgtcgaac
    20401 ggggataaat agctttcccg attgccttta gagcggagca agaatgaact atcgtattgg
    20461 tattctatag taaatccgca ctgtctgcag gtgaatagat tgcctgagct gtgcatggaa
    20521 gccctttctc cgcaactagg acaaatgtag agcgctaatt caaggctctc tgctcccttt
    20581 gtggttttga attgaaggct tttttgttta caccaagccg aatcatcata gcaaagtgca
    20641 tcgttgagca atcgatctat agttgagagc tccattttat gtagctgttc cggactaaac
    20701 agaagcgaaa acgagacttc aacttttcct tgccgtcgaa catgagacca tcgaggtttt
    20761 gcaagatacc cgccatttgt ctttaccaat actacaggga ctcgcaataa gcgtagcaat
    20821 tttgcactgg aaagaaaaac gggagcagga gtacctgtcc aagtgctctg accttcaggg
    20881 tacattccga ctacactatg cttatgcttt actagatcga cgatccaatt gacagtctcg
    20941 atatctggta tagcttttga ttttggaata cttcctacaa gcttgatcag cataaagcgc
    21001 atgatcggat ttctcatatt accatcactg gcaacccagt gtatgggaaa aggcacgaaa
    21061 gtgctggcga tgaagggatc aagcagcgtt gtatgatttc ctaccataat gaatggaggc
    21121 ttgagttctt taaagagttc ggtatttatt gcagtaatcc gatatttcca cttgagccaa
    21181 tttccatacg taaaccttaa aagccatgtg aagaacttaa aattagtcgg agagtatcgt
    21241 cggttgagaa tcataattgg caaccctgct cacgatcata atcatcgatt ctcggtatag
    21301 caaggtaggt cataaagata gtgttgcatg cctctggtat tttattaaaa tcttcctatt
    21361 aaccccatga aataaattca atcttgtgct atgtatacat tggaagaaga atccaaatta
    21421 accactatgg agtacgagga gcagtctatg ccttgtaaaa agcccctctc atcgaacaat
    21481 agctatcaaa aggcaagcaa tgacagtgta tcgaagacac tagagcgatg gctcgagatt
    21541 catgagcctg tcgacaaaga caaaactaaa gaggatccag aagcagatga cagattagat
    21601 tcaatttctg cctactccag atttaagcgc atgcttcctc aagacgaact agatctccac
    21661 gggttgtatg cggaagaagc tcgtcagatg atacaaacct ttatcgccaa tagtgcagcc
    21721 agaggccttg aaaaagtgag cattatacat ggaaagggaa atcattcgca ggaagagcag
    21781 gttctaatac atatagttcg agaagagctt gaaaagaatc cacaggcagg agaatttgat
    21841 tttgcagaca gtcggcatgg tggcaagggt gctacctggg tccgcattcg acctcctatt
    21901 tctcgcgata aataattcgt cctcggttca aatcgtatgg cgagaggcca acttttactt
    21961 tatcgcctgg cacaattctg atatagtgct tgcgcatttt cccggagagg tgcgcaagaa
    22021 taatgtgtcc atttgctaat tctacgcgaa acatcgtgtt aggaagagcc tcttttacga
    22081 tgccttctac ctcaatagct tcttctttag tcacatatac tccttgataa taggttttca
    22141 ggatgtcagc ccatcattga gggaatttcc atccgttctt tgcaagacta cggcttgcgc
    22201 aaaggccaca aaagaaaact cttgcagctg caatgcgaat gaaatagtat gaatcaatgc
    22261 atagaatgtc aacatgagga catctctaaa tatgtcttga catttttagc aatgtgatgc
    22321 attgtgccca tccactgcat actggttatt tgctattgcc agcaactgat gagggggaga
    22381 gagatggaga atgccttcat cgaaaaaatg aagaaactct tgctagccca gagacaagag
    22441 attttgaata caatcgccga atcgaataag gagctcagaa aagctctttc cgagaccgat
    22501 cccaaggatt cagcagatat cgcatctgat gatatcgata gaatcatgat cgaagccttg
    22561 agttcacaag atctaaagcg ctaccaagca atagaatcag cactccttcg aatcaaccag
    22621 ggtcgctatg gcctttgcgc aaagtgtggc aagaaaattc cagatgagcg tctagaggcc
    22681 attccctatg ccgtactctg cgtcgagtgc caaaaaagcg acgagcgaaa aaaccgctga
    22741 tctatcgacg ctggcctttc attgacgcta gagcggcttc tttccagaca ccgggagcat
    22801 ccatatacca cagcgcttta taccctgcta tctctgcaat ttccaaggct ttgagctgac
    22861 atataccaat atgtgaaggc gcatgtgcat catctccaat ggtaattctt atgcctttct
    22921 cctgcattct ttttaatatg aagactgacg gatagggctc gctgcgccta ctccgtgtaa
    22981 tacctcctgt atttatttct gcgatataat ctttttcggc agcaaggtcc agagcttgga
    23041 atgcagcatc cacataggct cgattttctt catcgaacca gcgtccagca atattatttt
    23101 tctttactaa gtcaaaatgc cctattatat cgaatccacc aacttctatc atttcagaaa
    23161 tatgccgcca atatcgcttc cagatttttc gataatcacc atctggtgcc cattcttgga
    23221 catgaagggc aaattgctcc tctggttcat caacagtaaa tggatattcg ccaggaacgg
    23281 tgatatagtg aaccgagcct atgcggtaat caagatgcat aggctcgtaa gctgggtcgt
    23341 taggcattgc tattcccggt gcgtattcgg tctctagacc caaaaatatc ttcatacctt
    23401 ttggtgcata ttcctgtgct aaagaacgga tggcagagac ataggaatca gccttatacc
    23461 aaggcaaatt gcaatctgtc tctaaggaaa ttggcgcatg ggcggaaaac cctagatggg
    23521 tgtattgatt ttcgaatgcg gtgcgagcca ttgtggctgc atctgccttt ccatcgcaaa
    23581 acagtgtatg ggtatgaaaa ctaaaggcca tgcgacatac tagcatcttt taatattttt
    23641 tttctatgtg ctatgtgggt gtaagaaaat tcaatgtaaa ggcaagcatg ctgcattttc
    23701 gtctattgac gcggttatag ccctttttta ctatgaaaga atgagcgaat ttactgccag
    23761 gtctgttgcg atctttttct cggtatatag cagcggagaa aaactcgcta gatctcttag
    23821 acaatgttgc actcatgtcg ctattctgag cggatattct gaagaaattt ctcgaaaatt
    23881 cgattgtatt attacggaga cagggttgct aaatgaaaga gaaatccata ctattcttga
    23941 ctcctcaact gctttggtgc ttatcgaaaa agccgtggga aagatgccgc aggctaaaaa
    24001 tgctgaatat tctttagagc agttttctaa ttgtgctctt ctaagagttt taccaaaaaa
    24061 cgcatctaac tcaacaatta aatgtgccgt ctatcagctc tttgcaaaga tggatagtat
    24121 cctttcgtct caaaacacta tagcagccgc gcagagtcaa ttgcggacac tgctatcctc
    24181 tcttccggat attgtatatg ttctggacga acaaggctat tttctctatc taaatgagtc
    24241 ggttggattg cttggctatc tgccttcaga gttgattgga aagcatttcg gctccattat
    24301 tcatccagaa gataggccaa acatctcgcg agatcttgtt gtggagaaaa taaggcaaaa
    24361 taatataatg cctacaaatc ctccaaaact cttcgatgaa cgaagatcag gttcgcgcat
    24421 gacaagagaa ctcgaagtga gacttctgca caaagatggc catgtaatct atgcaatggt
    24481 aaatgcttat ggagaacaag ccttcgatgt gccactcttt tcggatgtgg ttggcaaaaa
    24541 acttcagact atcggtgtta ttcacgatat cagcgcaatg catctctatc aagaaagcct
    24601 agaggaatca ctagctgcca aggagagact tctgcgcgaa atccatcata gaataaagtc
    24661 aaatctgcaa ttggtagcta gcttggctca cttaaagcag attgacacta aggctgcagc
    24721 ggctatcgag gtattgaaag acctagaaac gcagataaaa agcattgcgc ttgtacacga
    24781 agctctatat caatcggaaa gagtcgatcg attgtctgcg agggactttt ttggacaatt
    24841 ctgccatttc gcagaagaag ctctcgaaag aatagggtcc acagtacaca ttaaagtttc
    24901 tgctgaggat tatccactgg atccagatct ccttgttcct ctttcgctag tcttgctata
    24961 tctcttggaa ggcgcttata aggctagcta tgagagacgt gaaaaggtgg aagtttatct
    25021 ttcttttgtg caaaagcaaa atggtatgca gatcattgag ataaatgggg aggggatttt
    25081 tgagaatgca gattctattg tcatgcaagc attgctaaaa caagcccatg caaacttgga
    25141 aatacccaga ggagaggata gcggaaattt ttgccggctt attgtggaaa ctaagtctaa
    25201 aatgcgtggt atataatata taaaaattgt tatattaaat atatagagga gagcttgatg
    25261 ggtcagattt atgattttgc agttattgga gcaggtgcgg caggactctc cgccgctcag
    25321 tatggagcac gtgccaacct aagtacaatt gtaatcgaag aattagcccc tggaggtcag
    25381 gctcttttta tagacaaatt ggaaaactat cctggtattc tggagcccat taatggattt
    25441 gaacttgccg agcgaatgcg caagcaggcg gagcaattcg gcagctcttt tatcgaagca
    25501 agcgcattat cggtgagaaa agcggaagac cgctttataa tcgagacaag cgttggacct
    25561 ctagagagtc tcacggttct tatagctaca ggcgcaagac ataagcagct tggtattcct
    25621 ggagaacagg aatacatagg caagggtgtt tcttattgtg ctacatgcga tggtccattc
    25681 ttcaaaggaa agcgcattct tgtggtgggc ggaggcgatt ctgcctgcga cgaagcgatc
    25741 tatctctcca atctttcgga taaaatctta atgatccatc gtcgagatcg gttcagagcg
    25801 caaaaagctc tcgctagccg agttctctcc aatccttcga ttaaagtgct cttcaatacc
    25861 gttgcgaaag agatcaaagg aaactcaaag gtccactctg ttgtcctgga aaacaccctt
    25921 tcgcatgaat taaccgagga gccattcgat ggaatattca tatttatcgg ctctacacct
    25981 caaacgggct ttcttaaaga tatagctata caattcgatg aatcaggcta tatcataacc
    26041 gattgtaata tgcagacaag cattacaggt ctatttgctg caggcgatgt gagagcaact
    26101 ccatttagac aaattgtcac cgcagcagcc gatggcgcaa tcgcggctcg atcagctgcc
    26161 agctatatcg atgagcagag agggcaggct tatcgatgaa tagatgagag gtatcgatga
    26221 aaaaatcgat gaatttctgt acgctcgatt cagtgctcag aatcccgatt gaccttgtat
    26281 gcggtctgga tgaggcagga agaggcccgc ttgctggtcc tgttactgca gccgccgtca
    26341 ttctaccaaa aggctttccc attaccatgc tcgatgattc taaaagaatg agtgctcgtc
    26401 gccgcgaaga ggcttattgc atgattgtag agcaagcgct cgactgggct gtgggatggg
    26461 ctactgttga agaaatcgaa aagtacaaca ttcttatggc gagtttgcgc gctatggaaa
    26521 gggcttttag agggctatcg catcttccag cgcttgttgt ggtcgatgga ttatttgctc
    26581 cgtcccttag catgaatggg acaaatatcg aagcagcgac catggctaaa gcggatggct
    26641 tgattcccgc agtaatggcc gcttctatac tcgcaaaagt agccagagat cgcatgatgg
    26701 attgcctaga tggcatcatg cctgagtatg ggtttaaaaa caataaggga tatccaacta
    26761 aagagcatcg cgctgccata gagcgctttg gcccatcgcg attccatcgg aagggttttc
    26821 acttattgat tcaatccgat gcaccacttt ttgactctct ggaatctcca gaagaatgag
    26881 gtttctttct aataacaaat cgacgaggag cttgcgaaga gctttcctgc aatctctggc
    26941 gcgcttgtcg aggatcctgc ctaggctgag aagactgtat tgccgttcta tctggttttg
    27001 gcctttgact agctttttcc atatagccag ctgcggttct cattgctcgc atgaagcctt
    27061 ccatgttttc ttggaatatc acgattgatc gcctatcgaa ggtttctgtc tcagtaggtt
    27121 tgctctctac aatggacaga aaaatgtcgc catttctatt ttcttttaca ttaaaaaaat
    27181 aagttcttcc ctcacagatg aatcttgtag agaacagttc gccgcggata cccattagaa
    27241 ctccttttat atggaactat cctacattat tttgtctagg cgaagcaagc agatcggcct
    27301 tagcgaccaa tcctttgtgc catgtgcgca gccaagcttc gtcggcgtat gttccacctc
    27361 ttatatctac caaaatacgg aatacgggct ctgtccctga agctcgcatc cataggaatg
    27421 ctttaggttc gcctgcctta ttgtaaagta cgattctaag acctccattc ttggaagcgc
    27481 caaagtctga tccaatttcc cattcatcag atccaagaga ggcgaaggcc ctccatgaga
    27541 cgatgtcgaa cctttgctct agttctggct gtatttttgg ccagtcctcc agaaaaagtc
    27601 tttggtaggt atttttaagt tcgatcttat ctatggtacg aatttttagc gcagcattag
    27661 gttcgaaggc gctcgtagta atccattgtg gtagactttc tataacatct tcgagatcat
    27721 agtcaggctg gtagcgttcg gggctgccaa ttgcctcaag ccaaagatta aaacaggttt
    27781 catttttctg agcgtcgcca agccttaata atcgaatcat agagccaata gtggataatg
    27841 gatcgcgtac ctttgaagga tgagtaatat tgcctccatt cgagccttcc ccaagaatgc
    27901 gaaccgtcca tccttccaat ctaagccttt ccgcgcgaca gactacattg gcttcgcctg
    27961 tttcggttct aaatactttt gcgccgaaga cttctgctat ggcttcaatg cgcatacttg
    28021 ttgcatcatt gacgacgata gcgattcggt ttgtttcgcc tttccatttt agataagcta
    28081 gctcggatag gcaggccaag gcaaagacct gctgggcttc caaagggatc gcttctctaa
    28141 gcctattgtt gtaatacaca agattgcctc tatctccatc gcaatctggt acatagccaa
    28201 gagcataggc tgcatttttt tcgtgggcag attgtaaagc ttttatgcaa tcatcaagcg
    28261 attcgttttc aggtacgatt ctatgagcaa agatgccagg ttccatattt agcgctttag
    28321 attcggctcc gagagcagag agccaatcca aatcgatgct ctgtgttcgc gcagacccat
    28381 tgagctccgc aattatacca aaaggccgct tttcgcaagc atttgcgatt tccaaaagta
    28441 ttctttcttg agcctcggtt gtctcttcgt cggtgaagac actatgtgca aagagcatat
    28501 aagccgattg cgaataacgt ttccagtggg caacattctc atagcatttt gtcaaagctc
    28561 gagcgtcggc cttttcaagt gctataagcg ctagctctga agattcagga tctgatagaa
    28621 aaaccttgag ctctgagata agtggtttta tctgttcgct tttcagcaca cctccacaaa
    28681 ggccaaattt gacaccatta tatccaggag gattgtggct agccgagata tatacaaatc
    28741 catctgagtg gaattcatgg tccacatcta aagccgtagt ttgagcagca tatgccgtga
    28801 tttctggaat tggaactata aacagatagc gaacctcaca tccttctcca ataagcactc
    28861 gagcaaaaat gtcagcgata gtagggctcg tacgccttgt gtcgatacct aggagcacaa
    28921 taggcttgta gggtctacaa tgttctttaa taaatgaaga aaagactatt gccattcctg
    28981 ccactattac tatatccgca ggggctagaa gtggcgaaag agagttatct ggtagctgct
    29041 cataatccca agaggcatac cccgcggcaa tctcgggttt agcgaagatc tttcgccatc
    29101 ctgaggcaga cagaattagt tcttgagcag ctagtctaac atctttagag gtggggagct
    29161 tttctctaga gagaggtatc ttccccgggt ccccaatagg caaaccaaga aatgggtggc
    29221 taatcaatgg catgttttgc tcctggcaat attgtagctg tttttctcga ttctgataat
    29281 gataatgcca ttgatagaca gcgttgcaat agagtcataa aacagccaac ttctgtcagg
    29341 tcagggaaaa tgcttattta cctggattgc ctctggcgat atattgatta ttggaatgtt
    29401 cggtatcttt aagcattttt tattttttat tcgcggtgta aaaacttatg ctcttgttgg
    29461 tccttcgggg acaggcaaga gtttccgcgc aaagctcgta gcgcaaaaat atggcataga
    29521 actcataata gatgatggcc ttttaatacg tggtgatcag attcttgcag gccgctcggc
    29581 aaaaaaagat cctacttatc ttggtgccgt taaaactgcg cttttcgacg atcgagaaca
    29641 tcgagatcag gtggcaaaag ctcttcagcg agaaaaattc cggaagatcc tagtaattgg
    29701 cacaagcgag cgcatggtgc agaagatttg tgagcgcttg cagttgccac atccggtcaa
    29761 ggtgataaaa atagaggaaa tagccacaaa agccgaaatc gaaaaggcag tgcagtcgcg
    29821 caaggttgaa ggtaagcatg tcattccagt tccggctctt gaagtaaagc gcagctatcc
    29881 ttcaatattc tatgatagtg taagagtctt tctaaggcga agctttggcg taggcgcgac
    29941 tcttcccaaa ttatacgaga agtctgtcgt acgcccagag tatgcaaaac gtggcagagt
    30001 agctatttca gaagcagcgc tttcgcagat ggtagttcac tgtgtgagcg aattcgatcc
    30061 taatgtccgc attatgcgac tcgcaattcg caatgatact caaggttatc gaattacaat
    30121 tatgctcgaa gttccttttg gaacaaggct tccttcgaaa gtacatgcat tgcaggaata
    30181 tattgtcgac agcatcgaaa aatttactgg aattctagtt gcagaggtga atatcgtcat
    30241 agatagactg accgctcgcc cacagaaagg ccagaaatgg ggccaggcta ttgacggtcg
    30301 tgtttcctct gtgtagtatg cgcacatatg cggcagtacc tttaatgatt ctgcagtaat
    30361 cgtttccaag gagagaaaag gtgccctgtg gtagaaagcg aaagctccaa aagatagcga
    30421 cgcacaagag aaaaaagaaa agaagactga atcgccataa gacccgcaaa tgatcgcgcc
    30481 gcacttatgt tcttgaaaag aggccacccc ttacaaaagg gcggcctttt tatttcccgc
    30541 cttttatttc cggcttttac tgctatttga aacacttcca atagggattc gatttctatt
    30601 tgaactgaat aaataccgtt tcgatatttg aaacgccctt tcatcttttg cagatttcct
    30661 cttgcgtttc cttggacaca tggtatactt aagatcactg cccctaattc aggagaaatt
    30721 acatgcaatc cgtcgttctc gtcataaacc ctggatctac tagcacgaaa tttgcg
//
LOCUS       DAOSOB010000017        28994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1622_length_28994_cov_61.784374, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000017 DAOSOB010000000
VERSION     DAOSOB010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 28994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28994
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_28994_cov_61.784374"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PLS88_03145"
     CDS             <1..1056
                     /locus_tag="PLS88_03145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOU06376.1"
                     /translation="RGQNRAAIGGIRATHSDPAKIRICLESIRLFSSMQEKYGLDMEW
                     REGGYLYIAFDEATKQQFCALLKKQKAAGLDIEWVGPETIYRLAPGLRTDDLIGGTYS
                     PQDGYASPLMAASAFHYLALAAGVSFFFNTKVKAIHREGERISSIEAGGESFSADIFV
                     NAAGAKAADIAKIAGFDLPVFPDCHEAGVTEPVERFMEPMIVDVRSDAESGNYYFYQA
                     ATGQIVFCITPRPQIWGRDKDSTSTFLPLVVRRMLELYPRLRNLRVRRTWRGMYPMTP
                     DGLPIIGYPDMAVNFLQLVGMCGQGFMMGPGLGSIISRSIAASGCHERPEGFTDYGFL
                     FDELSINRSFDHAELLS"
     gene            1117..1527
                     /locus_tag="PLS88_03150"
     CDS             1117..1527
                     /locus_tag="PLS88_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOU06377.1"
                     /translation="MFQVPTKTQYAIRALVHLVHAKSASVASIAEAEHIPSKYLEAIL
                     GKLKNAGLLISERGRNGGYRLAKDPTLIPMSEVVQATEGEIRLVECVDNAAVCILGEG
                     CLPRHFWLGLKSAVDLYLESVTLAEIAGPETSST"
     gene            1539..2573
                     /locus_tag="PLS88_03155"
     CDS             1539..2573
                     /locus_tag="PLS88_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOU06378.1"
                     /translation="MKKVVVAWFIFLLLSFPASVFGEITLKLAHLNPQAPYEVVSAAM
                     AAVFKSEVESRSNGQVKVEIYPDGEVGKEVETLIQVKSGKVQSFISTSGAIAQFYPLI
                     DITNIPFSISSYNVAYNVYDEQFGKELAADIEEKAGFHVLGFGESGGFFAITNSKRPI
                     RSPGDLKGLKLRTMPIPLHQEIVRALGATPISIAWSELYTSLLAGVVDGQMNPVSIIV
                     MDRLYEVQRYLTLIDFIYSPYVWVINQEFYNSLSKELKIVVDDAAKIAILAGRGLSRI
                     IDSTERGLPMVSMKMQVYVPSKTAIKQFQDITVPAAKKFLLTQYKEEGRIWMDKFFAA
                     IDKAEKELGY"
     gene            complement(2643..3929)
                     /locus_tag="PLS88_03160"
     CDS             complement(2643..3929)
                     /locus_tag="PLS88_03160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOU06379.1"
                     /translation="MKLILAIKRRKICINKVRLSILGIVTCFVIFSLPAYPIEAINRL
                     GIEIPDPPSLNARSAILIDAATGTILYEKEADLRLPPASLAKLVTLHIAMEEIESGRL
                     SLDEIIEIDPQDCSPFIPYGSSLMYLQPGMKVSVRDLMLGAAVVSGNDAAYALARRIA
                     GSNEEFAKMMNNEVKALGFHNLSFVEPSGLSEFNLVTARDFAFFCKKYIELHPKSLKE
                     LHSIHSIRFPRPEHATSQYRPNGIVLQYNRNPLIFNYEGADGLKTGYIIEVGYNMAAT
                     AMRNGNRFIAVLLGGSSTPYADGATQRTKDTKALLDWAFANFATARPGYILPKPLRVW
                     FGKSIWVDPEPEGEAAATVPALLKDRVTARIETVKNIRAPIYKGQKLGEIVYEIDGIK
                     ISSVNLVSGQDISKGNIFRILLDGIQRIFAYLFGKL"
     gene            complement(3944..5344)
                     /gene="asnS"
                     /locus_tag="PLS88_03165"
     CDS             complement(3944..5344)
                     /gene="asnS"
                     /locus_tag="PLS88_03165"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOU06380.1"
                     /translation="MTLLPTIKQLLSMEAVGQSVTVRGWVRTKRETKQAVFLEVNDGS
                     CLSNIQCVLDSFLRQNQSTNESILRLSTGASVEIIGELVPSPAKGQRVEVKCQQLKLI
                     GEAPPERYPLQKKAHSLEFLREIAHLRVRTNTFAAVARVRNRMAFAIHQFFQERGFYY
                     IHTPIITTSDAEGAGAMFQVTSLDLEALSKSGTPIDYEKDFFGKKAFLTVSGQLNVET
                     YCQALSRVYTFGPTFRAENSNTTRHLAEFWMIEPEMAFAHLDDNIALATEFIQYLVAI
                     ALQDCAEDIAFFDQRIQPGLRESLERIVKTSFTRMSYTEAIRELEDSNAVFEFKPYWG
                     CDLQSEHEKFLTEKVAGGPVVVTDYPKEIKAFYMKQNEDGKTVAAMDVLVPRFGEIIG
                     GSEREWRLDLLEKRMEELGLAKEPYQWYLDLRRYGSTPHSGFGLGFERLLLYITGMTN
                     IRDVIPFPRAPRQADF"
     gene            5480..5968
                     /locus_tag="PLS88_03170"
     CDS             5480..5968
                     /locus_tag="PLS88_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HOU06381.1"
                     /translation="MRYNLTTAYFLWLISGFGALGFHRFYLGKTGTGLLWMFSGGLGG
                     LGCLYDLITLPRQVREANLAYAAREVVGYENYSQGSGFIPHMRPSETPERVILRLAKA
                     NNGMVTAGEVAIEANIAIEEAQRQLDNLAKKGIAQVRVRSSGVLVYFFPEFSKEDTDF
                     VD"
     gene            complement(6009..7025)
                     /locus_tag="PLS88_03175"
     CDS             complement(6009..7025)
                     /locus_tag="PLS88_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOU06382.1"
                     /translation="MNFRFIFMNSKTVYSYIKTKDKAKRPYRHRNLMKGALIELVLWL
                     LLAGCELFAPNNATFQGKKYAIVVGINEYINNDDFGDLSFCVADAKAMEAMLTDAGWK
                     VEPIEAESMESGNDKATKDNIKRALQEVPTDVSTFLFYFSGHGHVYRDQAYIVPSDVD
                     SSSYSRMISSMISATEFSEWLSKVEASNKLVILDSCYSGGFVDPGDCIDAAPGNIASD
                     QISSNIEMFLRFGELLAQNSASASKDPSTAPLVISAAGRNEESWEYSSNDGGYGIFTH
                     YFLQAAETSQFGSMKGDFDGDGILSCIEAYNYAEKAIEKDNRSNQLPHISGGLRDFAL
                     IDLH"
     gene            complement(7015..7662)
                     /locus_tag="PLS88_03180"
     CDS             complement(7015..7662)
                     /locus_tag="PLS88_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06383.1"
                     /translation="MHKAIGKARSKVLFFLLLLFSNLSTALTIGALPALEFGMGFADA
                     WTNTQSTATGYHNGWNVSAEANWIFRISLDNKDIHNAGKEIPPPVSIILSPGFHAQYI
                     DMSASNWLSDGSRYRAWSAIGLGPELDFGLEFVDSIMVHKNSLLFSISYLANFANYTQ
                     TTLYSAYNSWVLKVSWNTRIDHNTAFFAALPLEYAFRADGKSILSGLVMGIRYEF"
     gene            7873..8094
                     /locus_tag="PLS88_03185"
     CDS             7873..8094
                     /locus_tag="PLS88_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU06384.1"
                     /translation="MKNDPTNSSQNLAILRKGTVLRIIESKYSTSESDRGTLWFRIQE
                     AGQTGWVPALEVNTYSSENQARNAALRME"
     gene            8097..9779
                     /locus_tag="PLS88_03190"
     CDS             8097..9779
                     /locus_tag="PLS88_03190"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HOU06385.1"
                     /translation="MDFFKAWILPPLVGAIIGYFTNWLAIKMLFRPYKTIRFAGIQLP
                     FTPGLLPREKDKLAISLGETVSKELLTPEVITQRIRSPAIRQTIMRAIKSAFTAFLNQ
                     NSEQIFFQIRESSQEAAKDSEPSVLLLDSNSQELGGKAQSLDQITIDSEVSAFQRNIV
                     EALQHLVERKSIQSSIEGMFAEFLNRLGKLTFKELISKEQFIASVLKASSSLETKVLS
                     ESKHMQSFQPSISRSSNLNHLLRAAIELPPDATVMALSDALVPKAYSWFLDYIDNFFH
                     SEGFRSRLETEAQSFVKRALDRLGPIQRLFISIAGYEAKIAQAMPETIEDLIQTIERI
                     LKDPSTPKSISDALYKAVLSWRSKDEAGTTKTDSETYQHANDSISFKNRDILEQVVLS
                     LKDSTEELRQRAEFVYDSLSDLEFEEILGIRTNSEAISGFVFSIIVPATQISSMSASN
                     TIGAIFVEILKDMTKGKTFADILKIQDSDVEQLSASLTDGLLELIQARISILIDAFDI
                     TSMVSERIDSLDMKEVERIVLQVVKKELAWITWIGGILGGMIGFIQSIISTF"
     gene            complement(9751..11124)
                     /locus_tag="PLS88_03195"
     CDS             complement(9751..11124)
                     /locus_tag="PLS88_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOU06386.1"
                     /translation="MNIRHFSSIDGVFDFVLGYVNVEKGQATEFKLDRMYWMASQLGN
                     PHLGRLTVHVAGSKGKGSVATLIAKTLEASELRTGLYTSPHILSWKERITESGIEMPD
                     EFLLKAAEEVYSLVEGRTQDDFPGNELPTYFELTTLIAFCAFRIAGFDAQVIEVGLGG
                     RLDSTNIVSPDVCIITPIELEHTQFLGSTIPLIAAEKAGIIKRGVPICTIQPKEEALK
                     VILKKAEDMGASCLVVGRDISVSDIKVNTQGTCCKLSATDTTPPSLLRLLQGTSIEIS
                     TPLIGSIYAGNMAVSALALSQLDIDLKTEHILKGFASSSMLARFEIVSQSPFVVLDGA
                     HTPESIRNILATFLELSKAPRILLFGCAHDKKHEEIAKILSPYFNEIIITRPGTFKQS
                     EPAIVFESFQKQMSNNILIDCYLIEDTNSAITKALSDAKEKNACVLVTGSFYLCAEFK
                     KSRLCFE"
     gene            11244..12266
                     /locus_tag="PLS88_03200"
     CDS             11244..12266
                     /locus_tag="PLS88_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06387.1"
                     /translation="MRNIHAAEERQPAKSPIHRRGRGWGSPQKTFSQSDESTRIDQPL
                     HLHDKQVVVIIESEGAVFDIHRYWHEMAYLPSFIGCFGGNVDPSIVGEVWRTVALETS
                     LRGEHPIVILLAALRVLNTLFPSMQRAVIIKTLEKYSLSEKTLEDLFNMNEKHPAEML
                     IIDWFSMAEGLIQEIGRVPHFSTAEEFLHSAKYIAPRSTVLVYSHLAELSAMRMWQNA
                     GLGECFYRIAGRERGKPGEYLRAALSAGFERSSMVAIGSSATMFRASQLAGIRFYPIT
                     PGLEEDAWKILAEHWFPAYLRGEAWKIDMNAEPFYEQICQEFNVLQTSKTIASRLHLI
                     SSKFEE"
     gene            complement(12249..14477)
                     /locus_tag="PLS88_03205"
     CDS             complement(12249..14477)
                     /locus_tag="PLS88_03205"
                     /inference="COORDINATES: protein motif:HMM:NF009881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU06388.1"
                     /translation="MGYSDSGTTEPLAFRMRPRTLDEFVGQEHIVGQGRLLRRAIQKD
                     QLSSVIFAGPPGTGKTTLARIIANTTKSHFITLNAVLSGVADIREAIDQAKIYRDMYS
                     RKTILFVDEVHRWNKSQQDALLPWIENGTVILIGATTENPFFEVNRALLSRSRVFVLK
                     QLTKEDLFKIARQALEDKERGYGLYNIGFEDGALEHIIETADGDARSLLNALELAIET
                     SSSPWPPQKGSNLFISMKDAEESIQRRAVLYDKDGDYHFDTISAFIKSVRGSDPDASL
                     YWLARMIYAGEDPDFILRRLLILACEDIGLADPQAITVVNSCAQAFDRVGLPEGQLHL
                     VHATLYCALAPKSNSILCYFDALEAVQSEQAEVPDHLKDSNRDKEAFGHGEGYQYPHA
                     YREHWVAQQYLPPSLMGRLFYIPGSLGFEGSKRRDVLIRREAQLAIQPEDIRQIEDVN
                     LVWSRKGESRLHWRIRSESSATERLLAIRTAAFNALPLKPIDNIIIVDPRSGFYTMEA
                     LRRTREGKAFVLLNNKEARLELENLSKTEEEIIQPEVAIYEELTTSSAVSAALKCFLS
                     ELKPSFVILCEFCRVPIAIYEILAGLTECKENESFRLTAFDVDIEASSRLSSALIAME
                     NLNDKEKEFCSHFLAFENELGHGPTFTNFDSKICSTKEWFDKMKSTISAFDVQKTMLK
                     TSYSRPIDEKQLEAWLDASTFYGSNFRKAFSESETEMLKTLIEKFNRNPQWPLNILFF
                     EF"
     gene            complement(14509..15630)
                     /locus_tag="PLS88_03210"
     CDS             complement(14509..15630)
                     /locus_tag="PLS88_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06389.1"
                     /translation="MYKKSQSIFIAILIILTISSCDNTFSIFQSIGQEKKQLGAELFK
                     ATTVRAMGSDGTNYYALMSKVVYKPIAGENWNILSIDGDSDYFAPGFASDGSTIFVAK
                     ADSNNNLEDIYSSSDNGTSWKAMDAKNSIGSSVSVDWLKCENKTLFVAVHGSAGYSLF
                     YYDVASTSFKNAFDSTIPSLSEPLVDIVWDGISSSPAKFWLISSSKVYSGAEGSIAED
                     TSTSNNPALSNGLVGIASDGAGRILVSRSDGIVYSYASGSWTNFTVKSSTKLGPLILL
                     NQPSTSNRILVAKGTSTLGYMEYDETGLALYENGKDFVSTSQSIYNSTVRAKKVEGFW
                     QPASDLNTIFILLATGTEGSYALYRNTYSGGTWGGWIAE"
     gene            complement(15736..16332)
                     /locus_tag="PLS88_03215"
     CDS             complement(15736..16332)
                     /locus_tag="PLS88_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06390.1"
                     /translation="MSKRLLSIMLMISTLALTLSAQEKIEDPFEATRVKGDQSISIDI
                     GGFVPLFVLDNTGTPISPSNMSIGAAFSVQYRYMLGKNFAIGGSGAGSFVTTVGGGTL
                     FLAPIAITGAWIWSREPMEYEVSAELGMNIMRLYGNGLISPIAKFGGGISRFVDSSWS
                     IGFKLNWWFVPEIHLGAYSNLTSYANFLEFSIGANYHF"
     gene            complement(16351..16893)
                     /locus_tag="PLS88_03220"
     CDS             complement(16351..16893)
                     /locus_tag="PLS88_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06391.1"
                     /translation="MLKRKSFNWSLENIDDILMALPEGIVCGLFEYSNGQRIDYENRN
                     FLEHVQQALGRNAQEICKSFPDIFTFSSVEKYSDHTILKLDKAIDFQNLDIALGLFAE
                     DAGLEISDKASIWGNKMREGIWFGNANAISLDVAISFKKYELVLYLVELPEALLHGFH
                     FRAFARVYCKEKRHSYLKQD"
     gene            17068..17838
                     /locus_tag="PLS88_03225"
     CDS             17068..17838
                     /locus_tag="PLS88_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06392.1"
                     /translation="MKRKSEEISYFLIDTISRWSGVDCISMDKRSQQDELDPHYALVL
                     DVYYRRAIPVMEKRQILFDNPGAFESARGGTKDRFFLDEIPIRVEYKHIPSVQDLIEH
                     PLHHIKLLKNTGTYPLYRLLHFSLVFSRSDWIERMRSNLTNFPEEAWNTLFDSFAAKM
                     EHYLSDFGAASVSGNQFFRLMSHSGFLRYAGASVFMFNHVFEPSHAEFESQLKAQPRL
                     PANFVNLWDSIAGEKNDISEFKKFELARLLAREIFELR"
     gene            17960..19624
                     /locus_tag="PLS88_03230"
     CDS             17960..19624
                     /locus_tag="PLS88_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06393.1"
                     /translation="MLFLILIDSCKAKEVHAFEWYDEDGKRLAFTCSLIDKNVGIFEK
                     QGDKEKYNLIKPIKVSEGYSIRILVSSSSNETEFQLNIGDEKGKAKSIQCSIPDAGSY
                     AFVIPISEIEAIRWIEIKVLKLSSNISSGNIDSLKAASKDDSKAAMSESFNSPCLSIK
                     ELRFVPPFRGMQIENNITDISPHFFCENRGEKKRYEIIAPFSGLSSELSLKMKVEIKL
                     EGSTGLATFFWGNQKISYLHRGKESSFIIPCNIFENSPDKITMEVKDDIKVSAFFLSP
                     FKSVEEPPSIDPGLLVFHSPLVTNPFYLARWDQRPEVLLFLFKDYAVQDRYLKRLAFF
                     VEKMGFVGIIAQDSQIADLHGWNAHDYRAEDLARFFDAAAIQNFSLSDEEIELRELLI
                     KNGIILRSGSKYLPGRGALVSISQESPSYLQYRLLTHELSHALFFTDSRYRDLVISLY
                     QRLTNDEKWFLNRYFQWMRYNVNSSYLMANEMQAYLVQQPIQDLEKYFSKTLANRLIE
                     DHPELSDDISSYMEQHLDALMSCTEQLSSFLKSAYGFEPGMLFRVR"
     gene            complement(19732..20340)
                     /locus_tag="PLS88_03235"
     CDS             complement(19732..20340)
                     /locus_tag="PLS88_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06394.1"
                     /translation="MGKNASIPPQGRKHEDLLEGLFISPLDDSESLQKMEAEEKKILS
                     PLEGTAPLEGSREGSSANLFNSSEEKCVFPPDRMHESEKPEISTKKKPNRTESLAQEQ
                     LLLRFANELKSIKQDLISIKEHFEVIREPQPQPSIVPTSMEFPSDRASETQQKALIDE
                     VKKLLLYLDRLMESLPEEKIEEFANSEYFNLYRHIFEELGLS"
     gene            20446..20964
                     /locus_tag="PLS88_03240"
     CDS             20446..20964
                     /locus_tag="PLS88_03240"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOU06395.1"
                     /translation="MYSPHFSFKTSKRLLGSLSYGIFSFLIAYCFISFIAGKAGILAY
                     KDLALQKQQIQRAIDSLQAQNKNKASTIEELKNNPYAAADLAATLGYIHQGEVLIILP
                     ELWKDASQAKNDEVSTPILAGESTGLPDPLIRIMSAITGILAFLAIQLFHYKPIEQSR
                     KRIKIENQTELS"
     gene            complement(21010..23442)
                     /locus_tag="PLS88_03245"
     CDS             complement(21010..23442)
                     /locus_tag="PLS88_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOU06396.1"
                     /translation="MRLRKPKAIYAFIGLMLVLTAALGAGLGLALAETVNTIRTENFT
                     EFETALPTKIYDINGRLITEFFAEEQRIPISIKELPDHLIEAFITREDQSFYSHHGFN
                     LRSIMRAAIGQILGKNLGGGSTITQQLAGTLYADRRIFSLERKLKELWWALQLERRFT
                     KEEILEMYLNRMIMGPAVYGVEAASRYFFDHPAKDCTPAEAAILAIQLSSPSRYNPFR
                     NPMLARERSKEILDQMVARHVISRAEADESFDAYWASFDYSRVAVSAYYRREDKAPWF
                     SEYVRRQLEDTFYGSIDIYSDGLSVYTTLDLDKQAAADLYMKRGIETANASFKTATSQ
                     RLTEAERTYIPIIELLGLTFDIPTLYAPQSRNESASLNYFLEIINPILDATSLMLNIP
                     SLKPYVGAASAKINTELEKSTVEGALVTIDNNTGYILALVGGSDYSQANQLIRATQSN
                     IMPGSTFKPLYYSAAIDSRKITEGTYILDEPTTFYNEDGTPYTPQNFKGEWKGSVLAW
                     QALAYSMNVASVKVLQTVGFDAAISRAAALLDITDPIEIRKTFPRYYPLALGVIGVTP
                     LKMARAYAVFANGGKAITPIAIRYIEDRYGNIIAEPEKDAIQALRQKSPQVISSQNAA
                     IMIDMLKRVVSSGTLAGTTQSGSIFRQKTADGKSYVIPVIGKTGTTENWADAWTIGSS
                     PYYTTAVWLGFDRPGNSLGVSQTGATLAAPVWANYMRDIHLGLQYKNFSKPESGLVSA
                     TVCALSGQLPTEFCSDGTVNLLYLEGTEPTKFCELHGPVIAPEPIDSTEQMNIEEPML
                     SRPEESPVFQNR"
     gene            23642..24727
                     /gene="gap"
                     /locus_tag="PLS88_03250"
     CDS             23642..24727
                     /gene="gap"
                     /locus_tag="PLS88_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOU06397.1"
                     /translation="MKVAINGFGRIGRLVFQSIVDQNLLGHGKDKIDVVAVVDIVTDA
                     RYFAYQLRYDSVQGKMKADISSKKSDPQQAEDNILVVNGHEISCIMAEKELKNLPWRK
                     LGVDYVIESTGLFTDEKAYGHLEAGAKKVIITAPAKSKAEDKKIPTFLMGVNNDKYDP
                     AKHNIVSNASCTTNCLAPLVYVLLREGIGIETGLMTTIHSYTATQKVVDGVSKKDWRG
                     GRAAAINIIPSTTGAAKAVGEVLPEIKGKLTGMSFRIPTPAVSVVDLTFRSVKETSIE
                     EIDELLKKASNSYLKNILGYTDEELVSTDFIHDSRSSIYDSLATLQNNLPGEKRFFKV
                     ISWYDNEWGYSNRVVDLLRYMASKDNF"
     gene            24749..26008
                     /locus_tag="PLS88_03255"
     CDS             24749..26008
                     /locus_tag="PLS88_03255"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU06398.1"
                     /translation="MIKTVRDISLTGKRIIMRVDFNVPMKDGIVQDDTRIVAALPTIK
                     YILEQKPKSLVLMSHLGDPEKDAAKAKEKADKEGKPFDLEKYFAGKHRMKPVADYLSK
                     LLGVEVVFLPSCFGQKAAIDALPFGSVAMLENTRFHKEETAKDPTLQEVLAKELASYG
                     EIYVNDAFGSAHRAHASTATIAKFVPTAVGGLLMEKEVANLEPMLHNPPKPMVAIIGG
                     AKVSSKIGVLENLLKNASALIIGGGMAFTFLKAIGVTIGNSLVEDDFLETANMLLEKA
                     ANKDVKVVLPVDHIAADRFAPDAKPIIVDSQALPDNLIGLDIGPKTLALYAETLEDAK
                     SVLWNGPVGVFEFDAFAKGTEEVAKLVAEATERGALTVVGGGDSVAAVNKFGLASKMS
                     HVSTGGGASLEYLEGKVLPGIACLEQK"
     gene            26166..27092
                     /gene="cysK"
                     /locus_tag="PLS88_03260"
     CDS             26166..27092
                     /gene="cysK"
                     /locus_tag="PLS88_03260"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOU06399.1"
                     /translation="MHIYENITELIGNTPMLYLNELGKDLPCKIAAKLEFENPSHSVK
                     DRPALAMVLEAEKNGQLHSGMTILEPTSGNMGISLAMIAAVRGYRCLIIMPESMSLER
                     RAIMKAYGAEVILTPERQGMSGSIAEAKRLLSMYPNQYFMPMQFENPANPDAHRSSTA
                     QEILRDTDGKVDMVIAGVGTGGTITGIGEALKRIKPELEIIAVEPAGSPVLSGGSPGS
                     HGIMGIGAGFVPSILNTSIYNEVIAVSDEDSYVMARRAAKEEGLLVGISSGAVIYAAL
                     KIASRPQNRGKLIVGILASSGERYLSTPLFID"
     gene            27146..27892
                     /gene="cysE"
                     /locus_tag="PLS88_03265"
     CDS             27146..27892
                     /gene="cysE"
                     /locus_tag="PLS88_03265"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR01172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOU06400.1"
                     /translation="MKIGISVKKPSLLRIMRTDIQTVLERDPAARTRIEVLLTYPGLH
                     ALWSHRFSHFLWNRGSRLFARILSNITRCLTGIEIHPAAKIGSSFFIDHGMGVVIGET
                     AIIGKDVTLYHGVTLGGTSLEKKKRHPTIGDRVTIGAGAKILGDISIGDDSRIGANAV
                     VVKNVPPNSVVVGIPGQVIRRNIPHTAQDKPDLHHERIPDVIGQRISELMQRVSALEE
                     ALSAIQIKGIIEERTGDEEGQPDPIEHGAP"
     gene            28001..28750
                     /gene="tpiA"
                     /locus_tag="PLS88_03270"
     CDS             28001..28750
                     /gene="tpiA"
                     /locus_tag="PLS88_03270"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOU06401.1"
                     /translation="MKRYFIAGNWKMHKTIPEAIELASELKERLADCKEKLMIAPPFT
                     ALYSVAQVLKGSNILLGAQNMGPEESGAHTGEISVIMLKDIGVRVVILGHSERRHTYG
                     ESDALINAKVKLALKHDLEAILCVGETLEERERGVLENVIRRQLTEGLRGVDTSMLAN
                     VTIAYEPVWAIGTGKTATPEDADAVHAFCRQVIAELYSSDMAKNIVIQYGGSVKPENT
                     ATLMAKKNIDGALVGGASLKAESFVPIAKFL"
     gene            28876..>28994
                     /gene="secG"
                     /locus_tag="PLS88_03275"
     CDS             28876..>28994
                     /gene="secG"
                     /locus_tag="PLS88_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOU06402.1"
                     /translation="MGFFSILLLVIFVIVCLLLIFFVIIQDEESDSIGGIFASG"
BASE COUNT         8245 a         5950 c         6489 g         8310 t
ORIGIN      
        1 agaggtcaga atcgggctgc tataggcgga ataagagcta ctcactcgga tcctgcaaag
       61 atacgtatct gtcttgagtc gataaggctt ttttcttcga tgcaggagaa gtatgggtta
      121 gatatggaat ggagagaggg aggttatctt tatatagcgt ttgatgaagc gacaaaacag
      181 cagttttgtg cacttctaaa aaagcagaaa gccgcaggtc tggatattga gtgggtaggc
      241 ccagagacaa tatatcggct tgcacctggg cttagaaccg atgatctaat aggaggaact
      301 tatagccccc aagacggcta tgcctcgcct cttatggccg caagtgcttt tcattacctt
      361 gcgttggcag ctggtgtatc ttttttcttc aatacaaagg taaaagcaat tcatagagaa
      421 ggtgaaagaa tctcttcgat tgaagcgggt ggcgagagtt tttctgctga catctttgtc
      481 aatgcagcgg gcgcaaaggc tgctgacatt gcaaaaatag ccggcttcga tctgcctgta
      541 tttccggatt gccacgaggc aggtgtgaca gaaccggtcg agcgttttat ggaaccaatg
      601 atagtcgatg tccgctcaga tgctgaatct gggaattatt atttctatca ggccgctact
      661 gggcagattg tgttctgtat tactcctcgt ccacagatat ggggtcgaga caaggattct
      721 acttcaactt ttcttccgct tgtagttcgt cgaatgcttg aactgtatcc taggcttcgc
      781 aatttgcggg tacggagaac atggcgcggc atgtatccaa tgactcctga tggtcttccg
      841 attataggct accctgatat ggctgtgaac tttttacagc ttgttggtat gtgcggacaa
      901 ggttttatga tggggcctgg tctaggcagc atcatttcgc ggtctattgc tgcaagtggt
      961 tgccatgaaa gacccgaggg tttcactgat tatggctttt tgtttgacga actttcgatc
     1021 aatcgcagct tcgatcacgc tgaacttttg agctgattga caaagccttc aaaaagagcg
     1081 atattttata ttcagactta tttagtagga attataatgt ttcaggttcc aacaaagaca
     1141 cagtatgcga ttcgtgctct tgtgcatcta gtgcatgcaa agtccgcaag cgtcgcaagc
     1201 atcgcagaag cagaacatat tccctctaaa tacctcgaag caattctcgg taaactgaaa
     1261 aatgccgggc ttttgatttc tgagcgtggt aggaatggag gatacaggct tgcaaaagat
     1321 ccaacgctta taccaatgag cgaagtggta caagcaacgg aaggcgagat aaggcttgtt
     1381 gaatgtgtcg acaacgcggc agtctgcata ttaggcgaag gttgtctgcc tagacatttt
     1441 tggctgggac ttaaaagtgc agtcgatcta tatctcgaat ctgttacatt ggcagaaatt
     1501 gctggacctg aaacgtcgtc gacttaaagg aggcatccat gaaaaaagtg gtggttgcct
     1561 ggtttatctt tttgctccta tcatttcctg cttccgtgtt tggagaaatc actctaaaac
     1621 tagcacacct gaatcctcaa gcaccctatg aagtagtatc agccgcaatg gcagcggtat
     1681 tcaagagcga agtagagagc aggtctaatg gccaagtaaa agtggaaatc taccccgatg
     1741 gtgaggtagg gaaagaagtt gaaacgttga tccaagttaa gtctgggaaa gtacaatctt
     1801 ttatctctac ttcgggtgcc attgctcaat tctatcctct tatagatatt actaacatcc
     1861 ctttttcaat ttcgagttac aatgtagctt acaatgtata tgatgaacag tttgggaaag
     1921 agctcgccgc tgatatagag gaaaaggctg gttttcatgt ccttggcttt ggagaatcag
     1981 gaggattttt tgctattacg aattcaaaga ggccaattag atcgcctgga gacttaaaag
     2041 gcctcaaact aagaactatg cctattccac tccatcagga aattgttaga gcattaggag
     2101 caactccgat ttccattgcc tggtcggaac tatatacaag ccttctagca ggggtagtgg
     2161 atggtcaaat gaatccggta tctatcatag tcatggatag attatatgaa gtacagagat
     2221 atttgaccct aatagatttt atatattctc cttatgtatg ggtcatcaac caagaatttt
     2281 acaatagtct ttccaaagag cttaagatag tcgtggatga tgctgcaaaa attgcaattt
     2341 tggcaggaag gggactatca agaataatag actcaaccga aagaggcctg cctatggttt
     2401 ctatgaaaat gcaggtatat gttccatcca aaacagcaat aaagcagttt caagatatca
     2461 cggtcccagc agcaaagaag ttcttgctta cccagtacaa agaggaagga agaatatgga
     2521 tggacaagtt ttttgctgca atcgataaag cagaaaaaga gttagggtat tgattgctgt
     2581 atctttctca attgtccaac taagagcagc gctttcctaa aattagatac ttttcaagca
     2641 gatcaaagct ttccgaataa ataagcaaat atgcgctgta tgccatccaa gagaattcta
     2701 aatatgttac ctttcgaaat atcctgacca gaaactaggt tgaccgaaga aatctttatg
     2761 ccatctattt cataaacaat ttcacctaac ttttgccctt tatatatagg agctctgatg
     2821 tttttgacag tttcgattcg agcagttact cggtctttga gtagagcagg aactgtagcc
     2881 gctgcctcac cttcaggctc tggatctacc caaatcgatt tgccaaacca tacacgaaga
     2941 ggctttggca aaatatagcc aggtcttgca gtagcgaaat tcgcaaatgc ccagtcgagg
     3001 agggcctttg tatcttttgt gcgttgggtg gcaccatcag catagggggt agatgaccct
     3061 cccaaaagca ctgcaataaa gcgattaccg tttcgcatcg ctgtggcggc catattgtat
     3121 cctacttcga ttatatagcc cgtttttagg ccatcggctc cttcgtagtt gaatataagg
     3181 ggattgcggt tgtattgaag aacgatacca ttggggcgat attgcgatgt tgcatgttca
     3241 ggtcgaggga atcgaattga atgtatcgaa tgaagctctt tgagcgattt aggatgcagt
     3301 tcaatatatt ttttacagaa aaaagcaaag tctctagcag ttacaagatt gaactccgaa
     3361 agtccgctgg gttctacaaa gctgaggtta tggaaaccca atgcttttac ctcattgttc
     3421 atcatcttag cgaattcttc attcgatcct gcaatccggc gagctagtgc ataagctgca
     3481 tcattccccg atactacagc cgcccccagc attaaatccc gcacactaac cttcattcct
     3541 ggctgcagat acataagtga agaaccgtaa ggaataaaag gcgaacaatc ttgcggatcg
     3601 atctcaatga tttcgtccag ggaaagtctc cctgattcta tctcttccat agcgatatgg
     3661 agagtgacga gctttgccaa gctggcaggc ggtagtctaa gatccgcttc tttttcatat
     3721 aagatagtcc ccgttgcagc atctatcagt atggctgatc tggcatttag cgaaggtgga
     3781 tctggaatct ctatgcctaa tctattgatt gcttcgatcg gataggctgg aaggctaaaa
     3841 atgacaaagc atgtgacaat ccctagaatt gaaagtctga ctttgtttat gcatattttg
     3901 cgtcttttga ttgctagaat aagcttcatc gatgttttcc cgattagaaa tcagcttgac
     3961 gcggcgctct tgggaaaggg ataacatcgc gaatgttcgt cattcccgtt atatatagga
     4021 gaagtcgctc gaatccaagc ccaaagccgg agtgaggggt ggaaccatag cgccgtagat
     4081 ctaggtacca ttggtagggc tctttagcga ggcccaattc ctccattcgt ttttcgagaa
     4141 gatcaagtct ccattctcgc tcggaacccc cgatgatttc gccaaaacga ggcactagca
     4201 cgtccatggc tgccacagtc ttgccgtcct cattctgttt catataaaag gctttgattt
     4261 cttttgggta gtctgttacc actactggcc cgccagcaac tttctctgtt aggaattttt
     4321 catgctcgct ttgaagatca cagccccaat agggtttaaa ttcgaaaacc gcatttgagt
     4381 cttctagctc tcgaattgcc tcggtatagc tcattcgagt aaagcttgtc tttacaattc
     4441 gttctaagga ctctcttaag cctggttgta tgcgttgatc gaaaaaggct atatcttctg
     4501 cacaatcctg aagggctatg gcaacgagat attgaatgaa ctcagttgct aaggctatgt
     4561 tgtcatccag atgagcgaat gccatctcgg gttctatcat ccagaattca gcaaggtggc
     4621 gtgtggtatt agagttttca gcacgaaatg tagggccgaa agtgtaaact cgcgaaagag
     4681 cttggcaata tgtttcgacg tttagctgcc cagataccgt caagaaagcc tttttcccga
     4741 aaaagtcctt ctcgtagtct atgggagtgc ccgatttcga gagtgcttca agatcgaggc
     4801 tagtaacttg aaacattgcg ccagctccct cggcatcgct ggtggttata ataggtgtat
     4861 gtatgtagta aaagccacgt tcctggaaaa attggtggat agcgaatgcc atccgatttc
     4921 gaacacgagc tacggcagca aaagtatttg tcctgactct caagtgcgca atttcgcgta
     4981 aaaattcgag gctatgtgct tttttctgaa gcggataacg ctctggaggt gcttcgccta
     5041 tcagtttaag ctgctggcat tttacctcaa ccctttggcc cttagcagga gagggaacta
     5101 gctctcctat tatctccacg cttgctcctg tcgataatcg caaaatgctt tcatttgtag
     5161 attggttttg acggagaaag gaatctaaaa cacattggat attcgataaa caagaaccat
     5221 cgtttacttc gaggaaaaca gcttgcttag tctcgcgctt tgtgcggacc catcccctga
     5281 cggttacaga ctgacctaca gcttccatgg aaagaagttg ctttattgtt ggaagaagag
     5341 tcatgaaaag ctccatagaa agagtggtgc atggcttttc gtcatgcgct aaaattcata
     5401 tactcctttt tagcacgaca gagccattat ggtcaaatac catcgggctt gattgctaat
     5461 tttagcttgg gagaagatag tgagatacaa tcttacaaca gcctatttcc tctggcttat
     5521 ttcggggttt ggagccctgg gattccatcg tttctattta ggaaagaccg gaactgggct
     5581 tttgtggatg ttttcgggcg gtctaggagg attgggatgc ctatatgatc ttattactct
     5641 gcctcgccag gttcgagaag caaatctcgc ttatgctgcg agagaggttg ttggctatga
     5701 aaactatagc caagggagtg gattcatacc gcatatgaga ccttccgaga ctcccgagcg
     5761 agttattctt cgcctcgcta aggccaacaa tggcatggtc acggctggtg aagttgcaat
     5821 cgaagccaat atcgctatag aagaggcaca gcgccagctc gataatcttg caaaaaaagg
     5881 catcgctcag gtgcgagtgc gaagttccgg cgtgcttgtc tatttctttc cggaattctc
     5941 aaaagaagac acggattttg tagattgagt attgctcgga ctattgtcat gttttttgct
     6001 taaaggtctc aatgtaaatc tatcaaagcg aaatctctaa ggccacctga gatgtgaggg
     6061 agctgattgg atctattgtc tttttcgatt gctttttccg cgtaattgta ggcttcgata
     6121 caagagagaa tcccatcgcc atcgaagtct cctttcattg agccaaattg cgatgtttca
     6181 gcggcttgga gaaaatagtg agtgaatatt ccatagccgc cgtcattgct tgagtattcc
     6241 caggactctt cattccggcc tgcggctgag attacgagag gagctgttga aggatctttc
     6301 gaagcggaag cggagttttg agcaagaagc tcgccaaagc gcaaaaacat ttcgatattt
     6361 gaagaaatct ggtcagatgc aatgttgcct ggcgcagcat ctatacaatc ccctggatcc
     6421 acaaagccgc ctgagtagca agaatcgagg atgacaagtt tgttgcttgc ctctaccttg
     6481 ctaagccact cagaaaattc tgtagcggag atcatgcttg aaatcattct ggaataagaa
     6541 gaactatcga catcgcttgg cacaatgtag gcttgatctc tatatacatg accatgccct
     6601 gaaaaataaa aaagaaatgt cgatacatcg gttgggactt cctgcaaagc gcgttttata
     6661 ttgtctttcg ttgctttatc attgccagat tccattgatt cggcctcgat aggctccact
     6721 ttccagcctg catctgtcag catggcttcc atggctttgg catcagcgac acagaaactc
     6781 aaatcaccga agtcatcatt attgatatat tcatttatac ccactacaat tgcatatttc
     6841 ttaccttgaa atgtagcatt gtttggagca aaaagttcac agcctgctaa aagcagccat
     6901 agaactagtt caatcagagc tcctttcatc aggttacgat gcctgtaagg ccgttttgct
     6961 ttatctttcg tttttatata tgaatagaca gttttgctat tcataaatat gaatctaaaa
     7021 ttcatatctt atccccataa caagtccaga taagatgctc ttgccatccg ctctgaatgc
     7081 gtattccaaa ggcaaggctg caaaaaaagc tgtgttatgg tcaattctgg tattccaaga
     7141 tactttcaga acccacgaat tatatgccga ataaagcgtt gtttgtgtat aattagcgaa
     7201 atttgcaaga tatgatatag agaacagcaa gctattcttg tgtaccatta ttgaatcgac
     7261 gaattctaag ccaaaatcca actctggacc aagcccgatt gcactccaag ccctatagcg
     7321 cgagccatca gaaagccagt tagaagcaga catgtctata tattgggcat ggaatcctgg
     7381 tgacaatata attgagacag gcggtggtat ttcttttcca gcattgtgga tatccttgtt
     7441 atcgagagaa atacggaata tccaattcgc ttcggctgag acattccatc cattgtgata
     7501 tcctgtggca gtgctttggg tatttgtcca ggcatcagca aaccccatgc caaattctag
     7561 cgctggaaga gctccaattg tcagcgcagt gctaagattg ctgaataaaa gcaataggaa
     7621 aaataatact ttgcttcttg ccttgccgat tgccttatgc atagttattg tttaatacta
     7681 cacggttttc tttaaataat catcatgtgg gacatcagta tgaagagaat atttgcgtat
     7741 tcattaatta ttctagcctc ttcatttatc ttgggtgcta tctcagcttg taaaccgcgc
     7801 ttgcgactgc cagaagacag tagtcttggc ggaaatgcac aatggcttat aatatcttcc
     7861 cagtattgcc aaatgaaaaa cgatcccacc aatagttcac aaaaccttgc tattctccga
     7921 aaagggaccg ttctacggat tatagaaagc aaatatagta cgtcggaaag cgatcgagga
     7981 acactctggt ttagaataca agaagctgga caaactggat gggttccagc cttggaagta
     8041 aatacatact ccagcgaaaa tcaagcaaga aatgctgctc tcaggatgga ataaacatgg
     8101 atttttttaa agcatggatt cttccgccgc tcgtaggtgc cattattggg tattttacta
     8161 actggctcgc tataaaaatg ctttttagac catacaaaac aatccgattc gcaggcatcc
     8221 aattgccttt cactccaggc ttgctgcctc gggaaaagga taagcttgca attagtttgg
     8281 gagagacggt ctctaaagag ctcctgactc ccgaggtaat aacacagaga attaggtctc
     8341 cggcaatcag gcagacaatc atgcgagcaa ttaaatcagc ttttactgca tttttgaatc
     8401 agaattcaga acaaatcttt ttccaaatta gagagtcaag ccaggaagca gcaaaagata
     8461 gcgagccctc ggttcttctt ctagattcga atagccaaga attaggggga aaagcccaat
     8521 ctttagatca aataacaatt gactctgaag tttctgcttt tcaacgcaat atagtggagg
     8581 ctctgcaaca tttagtggaa cgcaagagta ttcaaagctc tatcgaagga atgtttgctg
     8641 aattcttgaa ccgacttggc aagcttacct ttaaagaact aatttctaaa gaacaattca
     8701 tagcatcggt gctcaaagct agttcttctt tagaaacaaa agtactttca gaatccaaac
     8761 atatgcagag ttttcagccc tcaatatcca ggtcttcaaa tctcaatcat cttttaagag
     8821 cagcaataga acttcctcca gatgcaacgg ttatggctct ctcagatgca cttgttccga
     8881 aggcatattc ctggtttcta gactatatag ataatttttt ccatagcgaa ggatttaggt
     8941 caaggctgga aacagaagct caatcatttg taaaaagagc attagataga ctagggccta
     9001 ttcaaaggct ttttatttct attgcaggtt atgaggctaa gattgctcaa gctatgcctg
     9061 agaccatcga ggatctaata cagactatag aacgcatctt gaaagaccct tccaccccca
     9121 agagtatttc tgatgccctt tacaaagcag tccttagctg gcgctcgaag gacgaagccg
     9181 gaaccacaaa gacagattca gagacatatc agcacgcaaa tgactccatt agctttaaga
     9241 acagagacat actagagcaa gttgttttat cgctcaaaga ttccactgaa gagttgcgac
     9301 agagggctga atttgtctat gactctcttt cggatctgga gtttgaagaa attttgggaa
     9361 tacgaacaaa tagcgaagct atttcgggat tcgtcttttc aatcatagtt cctgccacgc
     9421 agatttccag tatgagcgct tccaacacaa taggagcgat ctttgtagag atcttaaagg
     9481 atatgacaaa aggcaagact tttgcagata ttttgaaaat ccaagattcc gacgtagagc
     9541 aattatcggc atcgctcacc gacgggcttc tagagcttat tcaagcgcga atatcgattc
     9601 taatagatgc attcgatatc acttcgatgg tctctgagcg gatcgatagc ctagacatga
     9661 aggaagttga gcgcattgtc cttcaagtag taaaaaaaga attagcctgg attacatgga
     9721 ttggtggtat tctaggagga atgataggct ttattcaaag cataatctcg actttttaaa
     9781 ctctgcgcat agatagaatg agcctgttac tagaacacag gcatttttct cttttgcatc
     9841 gcttagagct ttagttattg cgctatttgt atcttcgatt aaatagcaat ctataagaat
     9901 gttatttgac atctgttttt gaaagctttc aaatactata gctggttcgc tctgtttgaa
     9961 tgtaccaggt cttgttatga ttatctcgtt aaaatagggg gatagtattt tagctatttc
    10021 ctcatgtttt ttatcatgag cgcatccaaa taataagata cgcggagcct ttgaaagctc
    10081 tagaaatgtg gcaagaatat tgcgtatcga ttctggagta tgagctccgt cgagcacaac
    10141 gaaaggagat tgcgatacta tctcaaatcg cgcgagcata gaagatgaag caaagccctt
    10201 aagaatgtgt tcagttttca ggtcgatgtc tagctgagaa agggcaagag cagagacagc
    10261 catatttcca gcataaatag agcctatcaa aggcgtgctg atttcaatcg aagtaccctg
    10321 cagaagtcga agtaggctag gaggagtagt atcagttgcg gataatttgc agcaagtgcc
    10381 ttgggtattg acctttatat cggacacaga aatatctctt ccaactacta ggcaggaagc
    10441 tcccatatct tcggctttct ttaagatcac tttgagcgct tcttcttttg gttgaatcgt
    10501 acagatcggt acacctcttt tgattatgcc tgctttttcg gcagcaataa gaggtatagt
    10561 cgagccaaga aactgagtat gttcaagttc tataggtgtt attatgcaaa catcgggtga
    10621 gacaatgttt gtcgaatcca atcgcccccc taatcctacc tcgataactt gagcatcgaa
    10681 accagctatg cggaaggcgc aaaaggctat tagggtagtc aattcaaaat atgttggcaa
    10741 ctcattgcca ggaaaatcat cttgagtcct gccttcgact aaggaataga cttcttcagc
    10801 agctttcagc aagaactcat ctggcatctc tatacctgat tcggtgattc gttctttcca
    10861 agaaaggata tggggagaag tgtagagtcc ggttcgcagt tcagaggctt cgagtgtttt
    10921 tgcaatcaat gttgcgaccg agccctttcc cttcgaacct gccacatgta cagtcaatct
    10981 tcccaaatgc ggattgccga gctgggaggc catccaatac attcgatcca gcttgaattc
    11041 ggttgcttga cctttttcga cattgacata gccaaggaca aaatcaaaaa ctccatcaat
    11101 ggatgaaaaa tgtctaatat tcacgcatag agcatgcgca atgcaattaa gttcgtcaag
    11161 agaggttgtt tgttgttcaa aatagatttg tgctttgata aaaggctagc ttcaacttgc
    11221 cgataagctt agatgaggat gatgtgagaa atattcatgc agcagaagaa aggcagcctg
    11281 cgaagtcccc gatacaccgc cgggggcgtg gttggggatc tccacaaaag acattttctc
    11341 aatctgatga atctacaaga atagaccaac ctctgcacct ccatgacaaa caagtagttg
    11401 taattatcga gtcagaaggg gctgtttttg atattcatag atattggcac gaaatggcct
    11461 atctgccttc ttttattggt tgctttgggg gcaatgtcga tccatcgatt gtgggtgaag
    11521 tatggaggac agtcgctctt gagacatctt tacgtggaga gcatccgatt gtcattcttc
    11581 ttgcagctct tagggttcta aatacacttt tcccttcaat gcagagagca gtgattatca
    11641 agactctaga aaaatatagt ctctcagaaa agacccttga agatcttttt aatatgaacg
    11701 aaaaacatcc tgcagagatg ttgatcatag attggttttc tatggccgaa ggtttgattc
    11761 aggagattgg aagagtaccc catttcagca cagccgaaga attcttgcac agcgcaaaat
    11821 atattgctcc tcgttcaaca gtgcttgtct atagccatct tgctgaactt tcggccatga
    11881 gaatgtggca aaatgctggg cttggagagt gtttctatcg cattgctgga agagaaagag
    11941 gaaagcctgg cgagtatctc cgagctgccc tttctgcagg ctttgagcgt tcgagcatgg
    12001 ttgctattgg ttcatcggca actatgtttc gtgcttctca gcttgctggc attcgctttt
    12061 atccaatcac tccagggctg gaagaagatg cctggaagat attggctgaa cattggtttc
    12121 cagcttatct tcgtggtgaa gcatggaaaa tcgatatgaa tgcagagcct ttctacgagc
    12181 agatatgtca agaattcaat gtcttgcaga cttcaaagac tatagcttca cgtttgcatc
    12241 taatatcatc aaaattcgaa gaatagaata ttcaaaggcc attgtggatt tctgttgaat
    12301 ttttctatta gagtcttgag catttctgtc tcgctctcgg agaaggcttt tctaaaatta
    12361 gaaccataga aagtcgaggc atctagccat gcttccaatt gtttttcatc tataggcctg
    12421 gaataggaag tcttaagcat agtcttttga acatcgaaag cgcttatggt cgacttcatt
    12481 ttgtcgaacc attcctttgt agaacatatt ttagagtcaa aatttgtaaa ggttggtcca
    12541 tgtccaagtt cattttcaaa agccaagaaa tgactgcaaa actccttttc cttgtcgttg
    12601 agattttcca tggctattag tgcgctagaa aggcgcgaag atgcttctat atctacatcg
    12661 aaagctgtta atcgaaagga ttcattttct ttacattctg taagcccagc taaaatctcg
    12721 tatatagcga tgggaacacg acagaattcg catagaatca caaatgatgg cttgagctct
    12781 gacaagaaac attttagtgc ggcacttaca gcactagagg ttgtaagttc ttcatatata
    12841 gcaacttcag gttggattat ttcttcttct gttttgctca ggttttcaag ctccaagcga
    12901 gcttctttat tgtttagcaa gacaaaagct ttgccttcgc gagttctcct cagcgcttcc
    12961 atcgtataga atccggatcg cggatcgaca attatgatat tatcgattgg cttaagtgga
    13021 agagcattaa aagctgccgt gcgtatggcg aggagtcgtt ccgtagccga agattcgctg
    13081 cgaatcctcc aatgaagccg agattcgcct tttctcgacc aaacgaggtt tacatcttca
    13141 atttgtctta tatcttcagg ctgtattgct agttgagcct ctcttcggat caagacatct
    13201 cgacgcttag aaccttcgaa tccaagacta ccgggaatat agaaaagtct tcccataagg
    13261 gaaggtggta aatattgttg ggctacccaa tgttctcgat atgcatgagg atactgatag
    13321 ccttctccgt ggccaaatgc ctctttatcg cggtttgaat cttttaaatg gtctgggact
    13381 tcggcctgtt ccgattgcac agcttctaag gcatcgaagt agcagagaat ggagttagat
    13441 tttggagcaa gtgcacaata tagagttgca tgtacgagat gaagctgacc ttcaggcaag
    13501 cccacacgat caaaggcttg ggcgcaggaa ttaaccaccg taatcgcttg gggatctgca
    13561 aggccaatgt cctcacaagc aaggatgaga agtcgcctca agataaaatc gggatcttct
    13621 cctgcgtata tcatacgcgc aagccagtat aggcttgcat cagggtcgga gcctcgtaca
    13681 ctttttataa aggcactgat ggtatcgaag tggtaatcgc catctttgtc gtatagcact
    13741 gcgcggcgtt gaatgctttc ctctgcatct ttcatgctga taaaaaggtt cgatcctttc
    13801 tgagggggcc aaggagaact gctggtttcg atagctagct ccaaggcatt gagtagactg
    13861 cgcgcatcac catctgctgt ctctatgata tgctcgagcg ctccatcctc aaatccaata
    13921 ttgtataggc catatcctcg ctctttgtct tcgagggctt ggcgcgctat tttgaaaagg
    13981 tcttctttcg taagctgctt gaggacaaag actcgagagc gcgagagcag tgctctattg
    14041 acttcgaaga agggattttc tgtcgtggct ccgattagaa ttacggtgcc gttttcgatc
    14101 catggcaaga gggcatcctg ttgactctta ttccatcgat ggacctcatc gacaaatagg
    14161 atcgtctttc ggctgtacat gtcgcgataa attttggcct gatcgatagc ttcacgtata
    14221 tcagctaccc ccgagagtac cgcattgagg gttataaaat gactcttggt tgtgttggca
    14281 atgatacgag cgagggttgt tttgccagtt ccaggtggac ccgcaaaaat aacggaacta
    14341 agctggtcct tttgaatggc gcgccgtagg agccttcctt gcccaacaat atgttcttgg
    14401 ccaacgaatt catcgagtgt gcgtgggcgc atgcgaaaag ctaaaggttc tgttgtaccc
    14461 gaatcactat agcccatgct ggaactaaaa agagggtgtg aagatctgct attctgcaat
    14521 ccagccaccc caagttcctc cggaataggt attccgatag agtgcatagg aaccctcggt
    14581 tcctgtagca aggagaatga atatggtatt aagatcacta gctggttgcc agaaaccttc
    14641 gactttcttt gcgcgaacag tgctattgta gatactctgg gatgtggaaa caaagtcctt
    14701 tccattttcg tataaagcaa ggccggtttc atcgtattcc atataaccta atgtcgaggt
    14761 tccctttgca acgagaatac gattagaggt agagggctga ttcaaaagga taagcggccc
    14821 aagctttgta gaacttttta ctgtaaagtt tgtccaactt cccgatgcat agctatagac
    14881 tatcccgtcg ctccttgaaa caaggatacg accagcacca tcggatgcaa tgccaacaag
    14941 accatttgaa agagcaggat tgttgctggt gctggtgtcc tcggctatgg aaccttctgc
    15001 tccgctatat actttgctag acgatatgag ccaaaatttt gcgggtgaag aagaaatccc
    15061 atcccagaca atatcaacta gaggctcgga aagactggga atagtgctat caaaggcatt
    15121 cttgaatgaa gttgatgcta catcataata gaaaagagaa tagcctgctg agccatggac
    15181 agccacaaaa agcgttttgt tctcacattt gagccaatca acgctaacac tagagccaat
    15241 gctattcttt gcatccatag ctttccagct tgtaccatta tcactactcg aatatatatc
    15301 ctccaaattg ttatttgaat ctgctttcgc aacaaatatg gtactgccgt ctgatgcaaa
    15361 ccctggtgca aaataatcgc tatcgccatc gatcgaaaga atattccagt tttctcctgc
    15421 tattggtttg tatactacct tcgacataag ggcataataa tttgttccat cggagcccat
    15481 agcgcgcacg gttgttgcct tgaaaagctc agcacctagt tgcttctttt cttgccctat
    15541 gctctggaaa atcgaaaatg tattgtcaca gcttgatata gtcaaaataa tcagaatagc
    15601 aataaagatg ctttgtgact ttttatacaa tgcgcaaata tcctttctag gatattctct
    15661 agctcttcta ttcaaaaaaa acttagattc tctttttcta atcttattaa gcatattaag
    15721 catgtaggca tctcctcaga aatggtagtt agcaccaatg gaaaactcta gaaaattggc
    15781 atagctagtt aaattgctat atgctccaag atgtatttct ggaacgaacc accaatttag
    15841 cttgaatcca atactccatg aagaatctac aaatctggaa atccctcctc caaatttggc
    15901 aataggcgaa atcagtccat taccatagag tcgcatgata ttcattccca gttcggcgga
    15961 aacctcatat tccatcggtt cacgactcca tatccatgcg ccggttattg caattggagc
    16021 gagaaaaagc gtgcctccgc ctacagtagt cacaaaagag cctgcacctg agccgccgat
    16081 tgcgaaattt ttccctagca tatatcgata ctgaactgaa aaagctgctc caatgctcat
    16141 gtttgatggt gatatgggcg ttccagtatt gtctagcaca aaaagaggca cgaagcctcc
    16201 gatatctatg cttattgatt ggtcgccttt gactcttgtc gcctcaaatg ggtcttcgat
    16261 tttttcttgt gcgctaagag taagtgccaa agtagatatc atcaacatta tcgacaaaag
    16321 tcttttcgac atcggtcctc cctgtatcat ttaatcttgt tttaaataag aatgcctttt
    16381 ttctttacaa taaacacgag caaaagctct gaagtgaaaa ccatgtaaca atgcttctgg
    16441 taactctact agatatagca caagctcata ttttttgaaa ctgatggcta catcgagaga
    16501 aatcgcattt gcattgccaa accagatccc ttcacgcatc ttattgcccc atatagaagc
    16561 tttatcagag atttccaagc cagcatcctc agcaaaaagc ccaagagcaa tgtctaggtt
    16621 ttgaaaatcg atcgctttgt cgagcttcaa tatcgtatga tcgctatatt tttccaccga
    16681 agaaaaagtg aagatgtcag ggaagctctt gcatatttct tgagcgtttc gcccaagagc
    16741 ttgctgcaca tgctccaaga aattgcgatt ctcatagtcg attctttggc cattgctgta
    16801 ttcgaataaa ccgcatacga tgccttcagg taaagccatc agaatatcgt ctatattttc
    16861 caggctccaa ttaaagctct ttcttttgag catccatatc tgttttctga tatttccatg
    16921 aggcctaaca attaatacga agtattgcat gttaattgag tatatattcc cttaggctct
    16981 tggtgaacat gtatgaaagg ttaatattca agatgcatgg cagaaagaca gcctccatgg
    17041 tataatcagt tttagaaggc agggtacatg aagcgcaaat ctgaagagat atcatatttc
    17101 cttatcgata cgatttctcg ctggtcgggg gtggattgta tatctatgga caaacgctca
    17161 caacaagatg aattagatcc acactatgca ttagtcctcg atgtatacta tcggcgcgct
    17221 attcctgtca tggaaaaaag gcagatactt ttcgataatc caggagcatt cgagtcggct
    17281 cgtggtggca cgaaggatag attttttcta gatgagatac ccataagagt agaatataag
    17341 catataccca gcgttcaaga tttgattgaa catccgctcc atcatattaa acttctaaag
    17401 aatacaggta cttatccact ctatcgtctg cttcattttt ctcttgtgtt ctcgagaagc
    17461 gattggatcg aacgcatgcg atctaatctt actaattttc cagaagaagc ctggaatacc
    17521 ctatttgaca gctttgctgc gaaaatggaa cactatctct ccgatttcgg agcggcatcc
    17581 gtgtcgggga atcaattttt tcgcttaatg tcgcactctg gctttctacg atatgcaggt
    17641 gcttcggttt tcatgttcaa tcatgttttt gaaccgtctc atgccgaatt cgaatcacag
    17701 ttaaaggctc agccgagact cccggcgaat tttgtaaacc tttgggattc aattgctggc
    17761 gagaagaatg atatttcgga attcaagaaa ttcgagctcg ctcgattgct tgcgcgagaa
    17821 atcttcgagc ttcgatagac aaatacctta aatagagatg ggcaacgtac aaataggaca
    17881 agagatagtt cgaccgactg cgaaaaaaaa taaaggtcta tttcgcaaga tactatctat
    17941 ttgtggattt ttatatttct tgttatttct cattcttatt gactcttgca aagcaaaaga
    18001 agtacatgcc ttcgaatggt atgatgaaga tggaaaaagg ctagcattta catgctcgct
    18061 aatcgataaa aatgttggaa tcttcgagaa gcagggcgat aaagaaaaat acaatttgat
    18121 taagcctatc aaggtttccg aagggtatag catacgtata ctggtttctt cttctagcaa
    18181 tgagacagaa tttcagctta atattggtga tgaaaagggc aaggcaaagt caatccaatg
    18241 ctcaattccc gatgcaggaa gctatgcttt tgtcattccc atttcagaga tagaagcgat
    18301 tagatggata gagataaaag tgctgaaact tagctccaat attagctcag gaaatattga
    18361 tagccttaag gcagcttcca aagatgattc taaagctgca atgagtgaaa gcttcaattc
    18421 gccttgtttg tctataaagg agctacgttt tgtacctcca ttccgcggta tgcagataga
    18481 gaataacatt acagatatat caccacactt cttctgcgaa aatagaggcg aaaagaaacg
    18541 ctacgagatt attgctcctt tttccgggct ttcttctgag ctttccttaa aaatgaaagt
    18601 tgaaatcaag ttagaaggaa gtactggtct agctaccttt ttttggggaa atcaaaaaat
    18661 tagctatctt catcgaggaa aagaaagctc ctttatcatt ccctgcaata tttttgaaaa
    18721 tagtccagat aagataacta tggaagttaa agatgacatc aaagtctcag ccttttttct
    18781 tagcccattc aaatcggttg aagagccccc tagtatcgat cctggattgc ttgtctttca
    18841 ttctccgctg gttacgaatc cattttacct tgcacgttgg gaccaacggc cagaagtcct
    18901 tttgttctta ttcaaggatt atgctgtaca agaccgttat ctaaagaggc ttgccttttt
    18961 tgtagaaaag atgggctttg tcgggattat agcgcaagac agccagattg ccgatctcca
    19021 tggctggaac gctcatgatt atcgagcgga ggacctagcg cgttttttcg acgctgcagc
    19081 tatccagaat ttttctctat cggatgaaga aattgaattg agagaactat tgataaaaaa
    19141 tggaattatc ttaagaagcg gaagtaagta tttaccgggc agaggggcat tagtatcgat
    19201 atcacaagaa tcacccagtt acctgcaata tcgcttgctt acacacgagc tgagccatgc
    19261 actttttttt acagacagtc ggtaccgcga ccttgtcatt tctctctatc agaggttgac
    19321 caacgatgaa aaatggtttc tcaatcgtta tttccaatgg atgcgatata atgtgaattc
    19381 atcatatttg atggctaacg aaatgcaagc ttatttagta cagcagccaa ttcaagattt
    19441 agaaaaatat ttctcgaaaa ctttagcgaa tagacttatt gaagatcatc ctgagctgtc
    19501 agatgatatc agctcttata tggaacagca tcttgatgcg ctcatgtcct gtacagaaca
    19561 actaagttct tttctaaagt ctgcttatgg attcgagccg ggcatgcttt ttagggtaag
    19621 gtagccgcag cagcgctgag gttattggcg ctatatataa aacttgattt taggctcttt
    19681 atgtatgctt tgattgttga agatttttcg aataatagct tacaagattg cttaagaaag
    19741 gcctagctct tcaaatatgt gcctatagag attgaaatat tcagaatttg caaattcttc
    19801 aattttttct tcaggcaagg attccattag acgatcgaga tagagaagca gctttttaac
    19861 ttcatctatc agagcttttt gttgggtttc agatgctcta tcagaaggaa actccatgga
    19921 tgtgggaact attgaaggtt ggggctgcgg ttctcttata acctcgaaat gttctttgat
    19981 ggatataaga tcttgtttga ttgacttcaa ctcattggca aatctcaata gaagctgttc
    20041 ctgagccaag ctttctgttc tgtttggctt tttctttgtg ctgatttcag gcttctcgct
    20101 ttcatgcatt ctatcaggag ggaaaacaca cttttcttca gagctattga aaagatttgc
    20161 gctagatcct tctctagatc cttctaaagg agcagttcct tctaaaggag agagaatttt
    20221 cttttcttcg gcttccattt tttgaagaga ttctgaatcg tcaagagggc ttataaaaag
    20281 gccttcgagc aagtcctcat gctttcggcc ctgtggtggt atactagcgt tttttcccat
    20341 cgcagtcttc ctctttaaga atactacacc ctttagctca tacagggaac tagttctcgc
    20401 aaaaatgcct catctgaatt agcatttgtg ccgaaaattt agctgatgta ttcgcctcat
    20461 ttttcattta agacaagcaa gaggctgctt ggctctcttt cctatggtat attctcattt
    20521 cttattgcat actgcttcat ctcttttatc gcaggtaaag caggaatatt ggcatataaa
    20581 gatctagcat tgcaaaaaca acaaattcaa agagcgatcg attcccttca agctcaaaac
    20641 aagaataagg caagcactat cgaggaactc aaaaataacc cctatgctgc tgcggatcta
    20701 gccgcaactt tagggtatat ccatcaagga gaagttttaa tcatacttcc ggaattgtgg
    20761 aaggatgcca gccaggcaaa aaatgatgaa gtaagcactc ccattttggc aggagaatcc
    20821 actggactac cagatccatt gattcgcatt atgagtgcca taacaggaat acttgctttt
    20881 ttggctatac agctttttca ctacaaaccc atagagcagt cgcggaaaag aatcaaaata
    20941 gaaaaccaga ccgagttgag ctgaaacgag aagcactgga tttagtcggc ctaaatctgg
    21001 cttaatttct tatcgatttt gaaaaacagg cgattcttca ggccttgaca gcatgggttc
    21061 ttctatattc atttgttctg tggagtctat aggctccgga gcaatgacag ggccatgtag
    21121 ctcgcagaat tttgtgggct ctgtaccctc aaggtaaagc agatttactg ttccatcaga
    21181 gcagaattcg gttgggagct gtccagaaag ggcacatacc gtcgcagaga caaggcctga
    21241 ttcaggcttg gagaagttct tgtattgcag gccaagatgg atatcgcgca tataatttgc
    21301 ccagacagga gccgcaagtg tagcccctgt ctgagaaact ccaagagagt tgcctggccg
    21361 atcgaagcca agccatacgg cagtggtgta atatgggctt gagccaatag tccaggcatc
    21421 agcccaattt tccgttgtgc cagttttgcc aataacgggg atgacatatg atttgccatc
    21481 tgctgttttt tgcctaaaaa tagagcctga ttgtgttgta ccagcaaggg ttcccgaaga
    21541 gacgactctc tttagcatat cgatcattat cgccgcattt tgcgacgaaa taacctgtgg
    21601 agatttctgt cgaagagcct gtattgcatc tttttctggt tcagcaatga tattgccata
    21661 acggtcttcg atatatcgta tggcaatggg ggttattgct tttcctccat ttgcaaatac
    21721 tgcatatgct cttgccattt taagcggtgt gactccaatt actcctagcg caagaggata
    21781 atatcgaggg aaggtcttgc gtatctctat tggatctgtt atgtcgagga gagctgctgc
    21841 ccgtgaaatt gcagcatcga agccaactgt ttggagcact ttgactgatg cgacattcat
    21901 agaataagca agggcctgcc atgcgagaac agatcccttc cattcgccct tgaaattttg
    21961 aggagtgtag ggggtaccat cttcattgta gaaagttgtt ggttcgtcga gaatgtaagt
    22021 tccctcggtg atttttctgg aatctatggc tgcagaataa tatagaggtt tgaaggtaga
    22081 tccaggcata atattcgact gggtcgcccg aatgagctga tttgcctgac tatagtctga
    22141 gcctccaaca agcgcaagga tatatcccgt attgttgtcg atcgtcacaa gtgcaccctc
    22201 taccgtgctc ttttcaagtt cggtatttat cttggctgaa gctgctccaa cataaggctt
    22261 tagagagggt atattgagca taagactcgt ggcgtccaaa attggattaa taatttcaag
    22321 gaagtaattc aacgaagccg actcatttcg actttgcggt gcataaagcg tcggaatgtc
    22381 gaaggtaaga cctaatagct caataatagg tatataagtt ctctctgctt cggtgagacg
    22441 ttgagatgta gctgtcttaa aggaagcatt ggcagtctca atacctcgct tcatatagag
    22501 atccgccgct gcctgtttat cgagatctag tgtggtatag acagatagac catcgctata
    22561 tatatcgatt gaaccataaa acgtgtcttc taattgacgt ctgacatatt cgctgaacca
    22621 tggagcctta tcttcgcggc gatagtatgc agatacagct acccttgaat aatcaaaaga
    22681 agcccaatat gcgtcgaaag attcatcggc ttcggctctc gagatgacat ggcgagctac
    22741 catttggtcg agtatttcct ttgaacgctc gcgagcgagc ataggatttc ggaaagggtt
    22801 gtaacgcgat gggcttgaga gttggattgc aagaatggct gcttcagcag gagtacaatc
    22861 cttggctgga tgatcaaaaa agtagcgact tgcagcttca acaccataga cagcagggcc
    22921 cattatcatg cggttgagat acatttcaag tatttcttct ttcgtgaaac ggcgctcaag
    22981 ctgtagggcc caccagagtt cctttagctt ccgctctaag ctaaaaatcc tgcgatccgc
    23041 atatagagtg cctgccagtt gctgagtgat tgtagatcca cccccaagat tcttgcctag
    23101 aatttggcca atagcggcac gcataataga tctgagattg aatccgtgat gtgaatagaa
    23161 cgactgatct tctctcgtaa taaaagcttc gatcaaatga tcaggcaatt cctttattga
    23221 aataggaatt ctttgctctt ctgcaaagaa ttctgtaatg agcctgccat tgatatcata
    23281 gatctttgta ggaagcgctg tttcgaactc agtgaaattc tctgtcctta tagtattgac
    23341 agtttccgcc aaggcgaggc caagtccagc tcccaatgct gctgttaaaa ccaacattag
    23401 cccaatgaag gcatatattg ccttgggctt tcgcaatctc atggcattag attatagata
    23461 ggatgaagtt ttggtcaatc ttaaataatt aaaaaggtat agatgttttt tagaaatttg
    23521 gatagattga tgcgaatcga ttgactcttt tgcctaatcg atactatttt atttttatta
    23581 taagctaatc acaggtgcct tttgagcgcc ttgtcaaact tagttcctga ggaggaacat
    23641 gatgaaagta gcaataaatg gctttggaag aattggaaga ttggttttcc agtctattgt
    23701 tgatcagaat cttcttggcc atggaaaaga caaaatcgat gtagtagcag tagtcgatat
    23761 tgtaaccgat gcccgatatt ttgcttatca gctgagatat gattctgtcc aaggaaagat
    23821 gaaggcagac ataagctcga aaaagagcga cccacaacaa gcagaggata atatccttgt
    23881 ggtaaatggc cacgaaattt cctgtatcat ggcagaaaaa gaactaaaga acctcccatg
    23941 gagaaaactt ggggttgatt atgtgatcga atccaccggc ttatttaccg acgaaaaggc
    24001 ctatgggcac ctagaagctg gggctaaaaa agtcatcatc actgcacctg caaaaagcaa
    24061 ggcagaagat aagaagattc ccacattcct catgggcgtt aacaatgata aatatgatcc
    24121 agccaaacat aatattgttt caaatgcttc gtgcacgaca aactgccttg ctcctctggt
    24181 ctatgttctt ctaagagaag gtataggcat tgaaactggt ctcatgacga ccatacactc
    24241 ctatactgca acacagaaag tggtcgacgg agtgtccaag aaggattggc gcggcggtag
    24301 agctgctgcc ataaacatca ttccttctac tactggcgct gcaaaggcag tgggagaagt
    24361 gcttccagaa ataaaaggaa agcttactgg catgtcattc cgtattccca cgccagctgt
    24421 ctcagttgtc gatttgacat tccgctcggt aaaggagact tccatcgaag agatcgatga
    24481 gctattgaag aaagcctcca attcctacct aaagaatatt ctcggatata ccgatgaaga
    24541 gcttgtttcc actgatttta tccatgatag tcgttcttcg atctacgata gcctagctac
    24601 acttcaaaac aacctacctg gagagaaacg attcttcaaa gttatttctt ggtatgacaa
    24661 tgaatgggga tattcaaata gggtcgtaga tcttcttcgc tacatggcgt cgaaggacaa
    24721 tttctaggat gcatctaagg agatctcaat gatcaagact gtccgagata tttcgctgac
    24781 aggcaagcgg ataataatgc gtgttgattt caatgtgccc atgaaagatg ggattgtgca
    24841 ggatgatacg cgtattgtgg ctgcgctgcc aactataaag tatatccttg agcaaaagcc
    24901 aaaaagtttg gttctaatgt ctcatcttgg agatccggaa aaggatgcag ctaaagccaa
    24961 agaaaaagcc gataaggaag ggaagccttt cgatctagaa aaatattttg ccggcaaaca
    25021 tcgcatgaaa cctgtcgcag attatctttc aaagcttctt ggtgtcgaag tggtctttct
    25081 gccatcttgt tttggtcaaa aagctgctat cgatgcgctt ccttttggat cagtagcaat
    25141 gctagagaat acacgcttcc ataaagaaga gacagcaaaa gatccgacat tgcaagaagt
    25201 cttggcaaaa gagcttgcgt cctatggtga gatatatgta aatgatgctt ttggatcggc
    25261 tcatagagcc catgcaagta cagcgacgat tgctaagttc gtaccaacag cggttggtgg
    25321 gctattgatg gaaaaagagg tggcaaatct tgaacccatg ctccataatc ctcccaaacc
    25381 gatggtggca attatcggag gagcaaaggt atcatcgaaa ataggcgttc tcgagaacct
    25441 actgaagaat gcttctgcct tgataatcgg aggaggcatg gcctttacct ttctcaaggc
    25501 aataggagtg actataggaa acagtctggt ggaagacgat tttctcgaaa cagcaaatat
    25561 gcttcttgag aaagctgcca ataaagatgt caaagtggtt cttccggtgg atcatattgc
    25621 tgctgaccgt tttgcacccg atgcaaagcc catcatcgtg gactcgcaag cattgcccga
    25681 taatctcatc ggactcgaca ttggtccaaa aacactagct ctgtatgcag agactctcga
    25741 agatgcaaag agcgtgctat ggaatggacc tgttggggtt tttgaattcg atgcttttgc
    25801 aaagggcaca gaggaggttg ccaaacttgt tgcagaagca acagagaggg gagcccttac
    25861 ggttgtggga ggaggcgatt cggttgcggc tgtcaataag ttcgggcttg cttccaaaat
    25921 gagccatgtt tctacaggcg gcggagcctc tctagaatac ctcgaaggca aagttcttcc
    25981 tggcattgca tgtcttgagc agaaataggc ctccctttcg gtttagcaag catatatagc
    26041 ggccgcccga aagggtggct gcttttttat atatgatcga gttaagcgcc gctctatatt
    26101 agacgctcct ctatatgggt cgttattcat ggcaactgcc atagatttac taaaggagtc
    26161 caaaaatgca tatatatgag aatataacag agctaatcgg taatactcca atgctctatc
    26221 ttaatgagct tggtaaggat ttgccatgca agatagcagc aaagctagaa tttgagaatc
    26281 caagtcattc agtaaaagat agacctgcgc ttgccatggt gctagaagcc gagaaaaatg
    26341 ggcaattaca ttctggaatg actattctag agccgactag cggtaatatg ggcatttcgc
    26401 tcgccatgat tgcggcagta cgtggctata gatgcctaat tatcatgcct gagtcaatgt
    26461 cgcttgaacg ccgagccatt atgaaagcct atggagcaga agtgattcta acgcctgaga
    26521 gacagggaat gtctggatcc attgccgagg ctaagcgact gttatctatg tatccaaatc
    26581 aatattttat gcctatgcaa ttcgagaatc ccgctaatcc cgatgctcat aggtctagca
    26641 cggcacaaga aattctccga gatacagacg ggaaggtcga tatggtaatt gcgggagtcg
    26701 ggacaggagg gacaatcact ggaataggtg aagcattgaa aagaataaaa ccagagctag
    26761 aaataatcgc agtagaacct gcggggtctc cagtgctctc cggcggatct cctggatctc
    26821 atggcattat gggaattggt gcaggattcg taccatctat tttaaacacc tccatctaca
    26881 atgaagtaat agcagtcagc gatgaagatt cttatgtcat ggctagacgt gctgcaaagg
    26941 aagaaggatt gctagtcgga atttcttcag gagctgtcat ctatgctgca ctaaagattg
    27001 cttctagacc tcaaaacaga ggaaaactaa ttgtgggaat ccttgcttct agtggagaac
    27061 gatatctttc tacaccgctc tttatagatt agaaagcttg atagcataga gaagtgtaga
    27121 gaacagagat tgtagaggat ttcaaatgaa aataggtatt tctgtcaaga agccatcgct
    27181 tctaagaata atgaggacag atatccaaac tgttctcgag cgagatccag cagccaggac
    27241 aagaatagag gtattgctta catatccagg tttgcatgca ctatggagcc atagattttc
    27301 tcattttttg tggaatcgag gatctagact ttttgctcgt attctctcga atattacaag
    27361 gtgtctaaca ggcattgaga ttcatcccgc ggctaaaatt ggctcaagct tttttatcga
    27421 tcatggaatg ggtgtcgtga ttggtgaaac cgctataata ggcaaggatg taacccttta
    27481 tcatggagtc actcttggag gaacttctct cgaaaagaaa aagcgccatc ccactattgg
    27541 agatagagtt actattggag caggagcaaa gattttagga gacataagca ttggcgatga
    27601 ttcgcgaatt ggagctaatg ctgtggttgt aaagaatgtt ccgcccaatt cggtggtcgt
    27661 tggtatacca ggtcaggtaa ttaggcgaaa tatacctcat acagcccaag acaagccaga
    27721 tcttcaccat gagagaattc cagatgtaat tggacaacgc attagcgaac ttatgcagag
    27781 agtaagcgca cttgaagaag cactttcggc tattcagata aaaggaataa ttgaagaaag
    27841 aactggagat gaagaaggtc aacctgatcc tattgagcat ggagcgccat agctttacca
    27901 aaaaaatcga taagtatagt attcatgagc taaggctgca cctctttagt taaacgagga
    27961 cagctttata ccagcattaa gtcgagggag aaataatagg atgaaacgct attttattgc
    28021 aggcaattgg aaaatgcata agaccatacc agaggctatt gaactggcat ctgagttgaa
    28081 agaaagatta gcagattgca aagagaaact tatgattgct ccgccattta cggcgctata
    28141 ttcggttgct caggttctaa agggtagcaa tatcttgctt ggagcacaga atatgggtcc
    28201 cgaggagagt ggagcacaca ctggtgagat ctcggttatc atgctgaagg acattggtgt
    28261 tcgagtggtc atcctgggtc actcagagcg cagacatact tatggcgaga gcgatgcgct
    28321 tataaatgca aaggtaaagc ttgcactaaa gcacgatcta gaagcaattc tctgtgttgg
    28381 cgagacgctc gaggaacgcg agcggggcgt tctagagaat gtaatacgac ggcagctgac
    28441 cgaaggtctc agaggggttg atacctccat gcttgccaat gtcaccattg cttatgagcc
    28501 tgtctgggct attggcactg gaaaaaccgc tacacccgaa gatgccgatg ctgtgcatgc
    28561 tttttgtcgt caagttattg ctgaacttta tagctctgat atggcgaaaa acatagtaat
    28621 acaatatggt ggttccgtaa agcctgaaaa taccgccact cttatggcaa agaagaatat
    28681 cgatggcgca ttggtggggg gtgcaagtct caaggctgaa agctttgtgc ctatcgctaa
    28741 attcctttga tttataaatt atctataagg tattgcttca atggagactt tgcgctagta
    28801 tagcttttcg gattgagaat atccctgaaa agttattttt ctgcagtcaa atgaacttgt
    28861 aactggagag agagaatggg tttcttcagc atattgctgc ttgttatctt tgtaattgta
    28921 tgcctcctgc ttatcttttt tgtgattatc caagatgaag aatctgattc gatagggggg
    28981 atttttgcaa gcgg
//
LOCUS       DAOSOB010000018        28448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1663_length_28448_cov_88.882260, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000018 DAOSOB010000000
VERSION     DAOSOB010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 28448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28448
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1663_length_28448_cov_88.882260"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLS88_03280"
     CDS             complement(<1..198)
                     /locus_tag="PLS88_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06403.1"
                     /translation="MKEKIIIVTAILVGAWIGSKLKLPSGYLTGGLILGLIVKGIVGG
                     NVPSSGSVSMISQLLVAYVLVA"
     gene            complement(218..1783)
                     /gene="citF"
                     /locus_tag="PLS88_03285"
     CDS             complement(218..1783)
                     /gene="citF"
                     /locus_tag="PLS88_03285"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOU06404.1"
                     /translation="MNYHCVRNSLGRLVPSEIPGLGTTTPFSGAFARLDGSFLWTASP
                     ISRRIPAISTNKVASNLQAAIERSGLQNGMTISFHHHLRNGDAIVGLVLSELEKMGFR
                     DLTLAPSSLGDSHDCVADAIKKGVVTRLNTSGVRGEVGKAISNGLLESPAIIRSHGGR
                     ARAIEEGSLKIDVAFLGAPACDRLGNMTGVIGNTACGALGYAMVDARYARHVIAITDN
                     LVDYPLSPKISIPQYLVDQVVVVDSIGDPAKIATGATRITKDPLQLYIAELAFKVIKA
                     SGYLKNDCSFQTGGGGPSLAVARYMRDYMKANSIKGSYCIGGITGYMADMLKDGLFKT
                     LFDVQSFDAAVTDSILHNPNHMEVDSSWYANPFNKGCLVNDLDVVVLAALDVDVDFNV
                     DVLTGHDGILRGASGGHSDTAAGAKLSLITIPSMRNGVPSIRERVQTVVTPGETVDVI
                     VTERGISVNPKREDLMARLKDSGIPIKDIHKLKEDIESITGIPDEVPFQDEIVGVVEY
                     RDGTIIDSIRKIK"
     gene            complement(1780..2682)
                     /locus_tag="PLS88_03290"
     CDS             complement(1780..2682)
                     /locus_tag="PLS88_03290"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOU06405.1"
                     /translation="MMNVFQSHPMRSLLYIPGNHPSMLQNCTIYGADAVIFDLEDSIS
                     ISEKDAARKLVKHALRNFDFGEISIIVRVNGRDTPFFEQDIEEIVPASPAAIRLPKVD
                     SAADVVAADELISRLEEINGLPKGKIGIQAMLETARAIVNVNSIAASSKRLVGLTLGG
                     QDLAADLGIRATKEGNELLYAKSAVIIAAKAYGLQAFDTVFTDVNDLEGLRAQTELSF
                     ALGFSGKAAIHPSQVSVINSVYTPDTKELAKAIKIVIAAREAEQRGLGVIAVDGRMVD
                     APVVSQALRTIELARLAGMEVAEI"
     gene            complement(2679..4169)
                     /locus_tag="PLS88_03295"
     CDS             complement(2679..4169)
                     /locus_tag="PLS88_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOU06406.1"
                     /translation="MHSILMNLSIGLQTAFTFTNILWVFIGGTLGTIIGMLPGLGPAT
                     GVAILIPITFGMNPATALITMAAVYYGAMFGGSRASIMLNIPGDASAIVSCFDGYPMT
                     KKGKAGTALAISAIASFIGGMIGMIFMIFLSQPVASAALRFGPAEMFSLMAFALVATI
                     TLSEGNLLKGLFSIVVGLMLSTIGIDPLAGVVRFTYGISSLNEGIDFLVVIIGVYAVT
                     EVYKNLKSIDTVYNFDSKSIGKVRVSKEEFKQCLAPMLRSTPLGFVIGVLPGMGGTIA
                     TFMSYAMEKNLSKHPEEFGKGTIEGLAAPEAANNACSCGAMVPLLTLGIPGSATTAVM
                     LGALMLLGVRPGPTLFVDHPDIAWGVLASMLIGNIILLFINLPLAIPLVQLLRVPQRI
                     MLPIVMGLGLIGAYFLNYSSFDLILVMISALVGYIFVILEVPLPPLVLALILGGTTEQ
                     SFRNAMVLSDGNPAIFFQKPISAVFIVLAGVSLVYALVKRRRQAAK"
     gene            complement(4181..4657)
                     /locus_tag="PLS88_03300"
     CDS             complement(4181..4657)
                     /locus_tag="PLS88_03300"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOU06407.1"
                     /translation="MTTNILTGIISIVLGLVYSITALRLPHMTMGDRLGPKIFPYFVG
                     IVTIITGIAMILQDRNPEKRSKKVDFGFKEHKNVWIKIGLLTIAGIIFGLVIDGLGYM
                     IATALFMFFVSMLINRGKLVQNIVIAVLFSVVTYFVFGVALKLSLPRGIIETMLPF"
     gene            complement(4746..5726)
                     /locus_tag="PLS88_03305"
     CDS             complement(4746..5726)
                     /locus_tag="PLS88_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOU06408.1"
                     /translation="MKKLSLFVLTVVLLGLVATVGAAPAWKPTKTIELIAPANPGGGW
                     DMLCRVMQKALTEEKLVDKPVIITNKPGGGGSVGWTYLKGKKGQGEYLAATSSLLILN
                     NLLGTSDLTYKDFTPIACMQAEWEAIVVRDDSPWKTVKDLMEAIKANPSAVPIAVGPS
                     LGNDDHLVFLQLARVYGIDPAKVKFIVYPGAGGEEVPALLGGHVKAMVIGLAEVLEQH
                     KAKKLRVIGVSSPERLELLPDVATIKEQGIDIVFPHWRGIIGAPGLTPEQVAYWDEVF
                     TKMVQTKTWKEQLENLLWDDYYQNSSTHAKFLEDQTKVFDELLSAVGLKK"
     gene            complement(5796..6506)
                     /locus_tag="PLS88_03310"
     CDS             complement(5796..6506)
                     /locus_tag="PLS88_03310"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU06409.1"
                     /translation="MSLLQEVPDLHIQPMREVIYERLRKAIVLGELQPDSYFTDADIA
                     HEFGVSRTPAREALQKLESNGYIERVPKKGNRILGISPYELAHSYAIRKALETLAVRY
                     SAIRITEAELVEMKEVLDKIYEIRQSMTGDELLENLLPSIKCFNEIAFEACKSSRLTE
                     AVWSEREIFDRYRVLRMVFSNRIDKSIHRRNELYKAFLAHDPDLACQIWTDHLNESFQ
                     IWREKSGNSEVLKDFQFF"
     gene            6644..8584
                     /locus_tag="PLS88_03315"
     CDS             6644..8584
                     /locus_tag="PLS88_03315"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOU06410.1"
                     /translation="MGKNIVQKLIDSHRIEGVQKSEFEDSEVLIKIDQTLTQDTTGTM
                     TYLQFEALGIDKVRTELSVSYVDHNTLQVGFENADDHNFLQDIASRYGMVFSRPGNGI
                     CHQVHLERFGVPGKSLLGSDSHTPTAGALGMLAIGAGGLDVALAMAGLPFSIPKPRVL
                     KVELKGKLSPMVSAKDIVLEILRRLTVKGGVGRILEYAGDGLSTLSIPQRGTIANMGA
                     ELGATTSVFPSDEVTKSFLEAQGRAKDFLPLFADPDATYDEEILIDLSSLEPMIALPH
                     MPDNVVPVREVAGLPVDQCFIGSCTNSSYYDLAMSAAILRGHHVAPKTSLVVAPGSRQ
                     VLLMTARDGILTSLVEAGARILECACGPCVGIGQAPATNAVSLRTSNRNFEGRSGTKS
                     ARLYLASAETTAASAIAGVITDPRTLESHPIVAMPESFLNDDSMFIFPSTEGETVGIR
                     RGPNIAPLPQFEAIGNELKAEVLMLMGDNITTDHILPAGSKIMSLRSNLPAISEYCFS
                     VLDANFPARARAAGKGILIAGQNYGQGSSREHAALSPRFLGVSAVIAVSYARIHRQNL
                     VNAGILPLIFDSPEDIENISQGDILCIKNIYEGLRLDRFEVKNESKGAVFMARHGLSQ
                     RQVEIILAGGALNAARRKLLVH"
     gene            8620..9720
                     /gene="citC"
                     /locus_tag="PLS88_03320"
     CDS             8620..9720
                     /gene="citC"
                     /locus_tag="PLS88_03320"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HOU06411.1"
                     /translation="MSETFFGLEERILHTKDELAQREALLTSRGLTVPEGEELVLGLF
                     DGEQLVATGALVRNVLQGIAVDMSHEGEGVAAAIVSSLIKRAVDRGITQIFLYTVSED
                     ISRFENLGFSKVAAVPKGAALLEWGSAGIESYLSSLKALAEDKPDHAGAVVINCNPFT
                     LGHRALIEYASSHVPWLYVLVVEEDRSLFPFRVRYRLVQEGVADLRNLTVMKGGPYII
                     SSATFPTYFIRPDANEASQRAVRMHASLDVSIFRMHIAPALKVANRFVGTEPYCPTTS
                     VYNEIMKIILQARETEAFGFDAKIPPVTVHEIPRFEIGGTPVSASKVRDMIREGNLSE
                     VQKLVPKTTWDWLKSDEAVPVMERIRQSDSRH"
     gene            9766..10035
                     /gene="citD"
                     /locus_tag="PLS88_03325"
     CDS             9766..10035
                     /gene="citD"
                     /locus_tag="PLS88_03325"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF009726.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HOU06412.1"
                     /translation="MRVLKSVTAGSLESSDVLVTIMPLSEDRIEYDIESIVIKQYGRR
                     IRKVTEDMVEIAKITGARIHVQDRGALECTLRARLETAIERSIAC"
     gene            10029..10598
                     /gene="citX"
                     /locus_tag="PLS88_03330"
     CDS             10029..10598
                     /gene="citX"
                     /locus_tag="PLS88_03330"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HOU06413.1"
                     /translation="MLSLQTVLAARELRWKNKKLLAESLAQKFPGHRLALAFCTLRMP
                     ASLRISNLYKEIVVQLRDVLAGALMDGAIQILDYGQEEYEDGPSAWLASDCDERILKR
                     MAIAIEEGHALGSLVDIDVTDAKARLLSRRDMGYAPRKCLVCDDEAAVCSAGQRHSLS
                     EIEAHIEAIISDSMGQEAWDSHDGHKERD"
     gene            10621..11598
                     /gene="citG"
                     /locus_tag="PLS88_03335"
     CDS             10621..11598
                     /gene="citG"
                     /locus_tag="PLS88_03335"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:TIGR03125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HOU06414.1"
                     /translation="MDRDELIAQIGNVALTATLYEVSAAPKPGLVDPFSRGAHSDMDY
                     FTFLASAVALAPFFAAFASMGAEIEGESPALLPELRKLGIEAERAMFAATNGVNTHKG
                     LIFSMGLVCAAAGRLLISKKQAESNDKNAMISGCAQEAADIVAGITVRELVPLVKADK
                     SLVSDPIKATNAMVCSLDLSAGEKSFVKYGIRGVRGEAEDGFPSVINHALPRLHSDLL
                     RGMSWNDAMIDALLVLYLIVDDTTVLNRGGSDGLAYIRAQAAAALSRGGMRLIEGRDF
                     VQSLDADFSQRGLSPGGCADLLAVTVFLEMLSNKWTTQQKANGSAQCSS"
     gene            complement(11638..12435)
                     /locus_tag="PLS88_03340"
     CDS             complement(11638..12435)
                     /locus_tag="PLS88_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU06415.1"
                     /translation="MNRKIIKLLFLSLLISVMSVFAGPSPSVTLKVGATSVPHAEILN
                     FIIPDLAAQGIKLEVIEFSDYVTPNIALAEKQLDANFFQHLPYLETFAAERKLSLESV
                     VAVHVEPLGLYSKKYKKVSDFPNGAIIAIPNDPTNEGRALIFLQSKGLIKLKPNAGLQ
                     ATVRDISENPKRFVFKEIEAPQLPRTLDDVAGSVINGNYALQSGFNPIKDSLLLEGGE
                     SPYANILVVRKGDSNDPRIQALAKALTSQKVKDFILSTYKGGVIPAF"
     gene            complement(12789..13796)
                     /locus_tag="PLS88_03345"
     CDS             complement(12789..13796)
                     /locus_tag="PLS88_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU06416.1"
                     /translation="MPENDEVLLDVRGLKMHFPVKTSMFSKTKNYIYAVDGIDFKVKK
                     GETLGLVGESGCGKSTTARAIDQLYKPTAGEVILKGKDLTKLSPNEMMEARKNMQMVF
                     QDPYASLNPRMTAGDIIAEPIRIFQKQGLIDMSTQDRDNRVEELMEKVGLSRFFKNRY
                     PHEFSGGQRQRIGIARALALNPELILCDEPVSALDVSIQSQILNLFKDLQEEFGLTYL
                     FIAHDLSVIKYISNRVAVMYLGLIVEIADAADLYKKPLHPYTEALLSAAPIPDPKIEA
                     KRKRIILTGDVPSPDKQRPGCNFYDRCPKRMDICKERRPSLKDAEDGHEVSCFLYHKS
                     E"
     gene            complement(13789..14781)
                     /locus_tag="PLS88_03350"
     CDS             complement(13789..14781)
                     /locus_tag="PLS88_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06417.1"
                     /translation="MSDEVILQVKDLKTYFNVDEGVVKAVDGVSFELHRSETLGIVGE
                     SGSGKSVTNLSIINLIPTPPGKIVGGEVLFHGTDLLKLPPSEIRHIRGNKISMIFQDP
                     MTSLNPFLRISTQMIETIQLHQGLDKKAAKEKAIEMLKFAGIPAPEKRIDQYPHQFSG
                     GMRQRVMIAMALSCNPEILIADEPTSALDVTIQAQILELIKELTRKFGTAVILITHSL
                     GVVAGMCDTICVMYAGRIVERGRTEDIFESPKHPYTMGLIRSVPRLDQETKVRLYSIP
                     GQPPNVIDLPDCCPFYPRCEQAMDICREKYPSIVSFENNQSVACWLYNKEIQNA"
     gene            complement(14794..16035)
                     /locus_tag="PLS88_03355"
     CDS             complement(14794..16035)
                     /locus_tag="PLS88_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06418.1"
                     /translation="MAKKQLENAVNEFNQVMKPVSLWADAWKRLRKNKMALISLVIVS
                     IYVVISLFAPLLTSTGILIDYRKQVITNASLPPSFKPAGELVVKKIENRIVDLEKRVE
                     EQQQQQSAFSSFFDFGSLDTQEESSEPSDGYTESQEGGFSFTYEGEGFSGNTTDPVVR
                     ELISQKETLERVKAELDTNPDYKIVYILGTDNLGRDMFARIIYGGRISIAIGIVGTLT
                     AALVGILVGAISGYVGGWLDNFLMRFVDIMYGLPYMLIVIIIMAMIGEKARGSFVVLF
                     VAIALVSWLTIARVVRGQIISLKNSEFVEAARSMGASTQRIIFRHLLPNTLGVIIVFS
                     TLMMPSFIMNESFLSFLGLGVSAPDASWGTLVSEGVRAMESYAWQLLCPGIAMTIFLF
                     CMNFLGDGLRDALDPQSKNRT"
     gene            complement(16046..16972)
                     /locus_tag="PLS88_03360"
     CDS             complement(16046..16972)
                     /locus_tag="PLS88_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06419.1"
                     /translation="MPRYFIRRVLSIIPTLFIVITLSFFLIRLAPGGPFAREREVPEA
                     ILQNLMKRYHMDEPLIKQYLRYMGDIIRWDFGPSYRYRDLTVNEIIDEGLPISMSLGV
                     ISLVLATVGGIAVGIISALKQNKWQDYVVTSIAVIGISVPLFVMGPVLQLVFGMRLKI
                     LPIGQWISTHGIKAVILPAITLSFPYFAYIARLSRASILEVLRSDYIRTARAKGLKES
                     VVVWKHVLKGALLPVVTYLGPAFSGIVVGSIVVESVFLVPGIGRPFVQSALNRDYTLI
                     MAEVVVYSIILIIANLVVDILYGFLDPRISYK"
     gene            complement(17203..18753)
                     /locus_tag="PLS88_03365"
     CDS             complement(17203..18753)
                     /locus_tag="PLS88_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU06420.1"
                     /translation="MKKILFALIALVAVLGLSAQDFTIVNGTEPTSLDPHAVEGVPEH
                     RIYVALFEGLTINDPRTNRALPAVAESWSFSKDYKTCTFKIRKGVTWSDGVEITADTV
                     VQSWVRKMDPKNAFQYADLPAMYIEGGMDYLEGKAGPESLKIRAVDKYTFEVKLVGPI
                     PFFADMVSHYAFAIVPMHAIEKYGKDWVKPGKMVSNGPYVLSEWKPQEKVVVVKNDKY
                     WDAKNVALKKITFIANDDINVGYNLYKSGAADWTEAVPLELMDEVKLRKDFHVAPQYG
                     TYYYILNTTRKPLDDPRVRKALAMAINKNDLVNKVTRGGQIPANSLVPPSTGYTPAKG
                     YDFDPAAARKLLAEAGYPDGKGFPAFTILYNTSSAHKNISEFVQAQWKVNLGINVSLM
                     NQEWGTYLDTRSQSHNFDIARAGWIGDYLDPSTFLEMFITGGTQNDGLYSNPKYDELL
                     NKARVTSGTERFQYLMDAEKILIDQDMAVLPLYFYVTQNLIDLDKWDGWYPNPLNIHP
                     WKFIRPKK"
     gene            19009..20187
                     /locus_tag="PLS88_03370"
     CDS             19009..20187
                     /locus_tag="PLS88_03370"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOU06421.1"
                     /translation="MYEFYAPTKILFGDGCLAEAASQLKEMNSTKVLIVTGRSKTASS
                     QGFKDLCLGLDSSGIHYVHFGEVGADPDTAIVDKGTEVYKAQRCNAIIGFGGGSPIDC
                     AKGIAASVAEDRPIRDFVGTGLAFTKPVPPLVAIPTTAGTGTEVTNAAVFTVVDESGH
                     RSKKGTSSQYYFPRLAIVDPVLHISMPPPLTAATGMDALTHAIEAYVSCFSTPISDMY
                     CLESIRLIGRSLRAACHISKGAEDEDIQSRRDMALAATLAGVALSQAGLGMVHGFAHP
                     VGALAGLPHGLANAIILPFVMQSLIPDAAERLSDIGKALTAKDNISPEESVRAICQLG
                     HDIGIPENLEAAGVPPVYFDDILSDALSYRRRKASPRAFVDAELRELLELIYHGVPQQ
                     "
     gene            complement(20197..23739)
                     /locus_tag="PLS88_03375"
     CDS             complement(20197..23739)
                     /locus_tag="PLS88_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU06422.1"
                     /translation="MKPLSLSFEHFGPYCKRQDIDFCKLDDFFLIYGKTGSGKTTIFD
                     AIAYALYGKAVGGRSNLERELGSRFSPAGSKPWVQFEFLASSAQWKVYRSLPYKKLNR
                     RGQESEAASEVTLYRMNEKGSYAILADRVTEANQILIDLLRLSAEEFSKIVLLPQGEF
                     QKFLEMKTTERVEILEKLFDVGIYDTITETARKKVMELDAYLKAKKEEIERISNELGI
                     EPESRIKESGKAISEFDEKIEELTRNALNLETQIAQKNERLSFLQSMLAACKSFHEKE
                     ELKPIFDERERKLEAAKSLGELASLARRVSADYEELIEILLEALQTGTELSMLETEQI
                     EIKEKKKALPFLRKDYDNLQRRAALYQSAVNAWNQKEQCEGECREVLQRLDAASEHWE
                     SRQKENARLKSSITELENALQGEPMAQESFQKIIIADEALKRIKTWIEQKENIEAQRS
                     ETLRKIQQTEDSILSIDQELAIAEQNRDRIDEEIRHAQAGILAASLIPGEPCPVCGAR
                     DHPNPAKLEQKALDDKTIKATQKVVEEIKSSRAALIQNKENLENRIEEFTQDLKNTIN
                     RISEEWNSIPCEFLGDEANIAFEQISSSMIELTGSHFQSQRRELEGRLKYYQHERETL
                     QKLRKTLDQVESDFIRIQEEKTGLEIKRAELDAHIASLEAQIGKEDPRIHLKEVEDNL
                     ADLHNEIEQIEERAQKWEISWSSANIKIDMQLETITQKGSALAITLEEFLHEAEKIEA
                     RRLLRLLESSVISSEKEAPINEEQTSWQLQGPLGSPNDFSGSLESPSRLSSRSLKELF
                     PKLDNVFLKIKRLKLSSERIIELSGTLESIFFAHTINEPALKELIEEVLCSVWTSDRI
                     KEEAREISAFRQSYIEARASYEVLAARSQALSPFNSFNSFNSTLLNQAPNLAVDRSDR
                     SLEREKENGEQSRHKEMLEPLYLEQQEKALREELDTLFEGKKNIELAISDLRDKRANF
                     ISELDQLRRLLSRREEVTREYDSAVEEFGKQEKLAKLLGGSLNPQRKMPFKNYVLGLQ
                     FREIAMRASERLYRMSNGRFLVEADILGGSGNQKIGLELYVLDAWNGGRRPVGTLSGG
                     EKFMLSISLALGLADSIQERAKANRIESLFIDEGFGSLDEESLSLAISVLEELRGDKS
                     IAIISHVDELYSRIPSRIVVQKAVGGSCLEFEQD"
     gene            complement(23736..>24433)
                     /locus_tag="PLS88_03380"
     CDS             complement(23736..>24433)
                     /locus_tag="PLS88_03380"
                     /inference="COORDINATES: protein motif:HMM:NF023739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HOU06423.1"
                     /translation="LNILVAHCYVRGAVLSESDIAFIGGTEAVPVSLFEAFDYVALGH
                     LHKMQALSPKVWYSGAPMPMSFGDEGIEKGFLYVELEDGEPARIERVELKPLHVFKRV
                     RASFAELMADSAFIDDDYVEVLLTDEEPVYDAFNRLALRFPNLMYVRQEAFEKRMGTE
                     VEKVGDIATLMPPNGETSSLDDTGNLAGKSLHTTVLNDFISFTQRILDEPPSQELLKA
                     FEVLLRNAEEELS"
     assembly_gap    24434..24533
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24534..25105)
                     /gene="sbcD"
                     /locus_tag="PLS88_03385"
     CDS             complement(<24534..25105)
                     /gene="sbcD"
                     /locus_tag="PLS88_03385"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HOU06424.1"
                     /translation="MSHSDKIFKIAHISDLHLGKILRSQDLVQDQAAILTQIVEKIRA
                     LKLDLVVIAGDVFDRSIPSESAQTMFGWFMAELKRALPQNGRILVIPGNHDSPKRIAF
                     AAELFESVGIHLVSEILLSPTLVLEKGGKRAAVWALPFVTHGTYSEFKRRLAEEGQVE
                     AGCTETISETISSMASRMESIIANLRPSIA"
     gene            25320..26300
                     /locus_tag="PLS88_03390"
     CDS             25320..26300
                     /locus_tag="PLS88_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU06425.1"
                     /translation="MIYRHLGSAGIKVSVISLGSWVTYGSQVDIDTAAQMIKYAYDHG
                     VNYFDNAESYAGGQSEVIMGQAFRRLGLRRGSYLVSTKLFWGIHEGPNEKNTLNRKYL
                     LEAIEGSLERLGMKYVDFLFCHRPDPETPIEETVWAMHDIISSGKALYWGTSEWSAEQ
                     IREAWEIADKCNLRKPQMEQPQYNMLVRTKVEKEFARLYQGIGLGLTTFSPLASGLLS
                     GKYKDGIPKDSRFALPGYEWLQERWYQPEIIASIERLRPIAESLGATMSQLGLAWAAH
                     NPNVSTVITGASRLSQLEENLGAIEILPRLNTEILDKIDEALEPILVMRN"
     gene            complement(26492..27625)
                     /locus_tag="PLS88_03395"
     CDS             complement(26492..27625)
                     /locus_tag="PLS88_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HOU06426.1"
                     /translation="MHKHTITAIICLAGAIVASALAGSSFLAMRKPDKIVSGPGVTEI
                     KMLSDWFPALKGSAGDTEVYIMKGSDDGVSMLVLGGTHPNEPAGHLAAILLIENFLPR
                     TGTLYVIPRANASGFTANDPQEGAPQRFTIETPNGPRVFSYGSRATNPVHQWPDPNVY
                     IHASSGQTLSGSETRNLNRGYPGRADGTLTEKVCYGIVQLIKEQNVTITVDLHEASPE
                     YPVINAIVAHPKAMQLASDTVLGLEMQDIKISLEPSPVNLRGLTHRELGDFTDTLPLL
                     METANASQGRLRGRTDEALVLTGKDKWYVRAAKLGRLYVPYDENGHPIEERVGRHVTA
                     IAEIINSWNWEHPETPLEFDNIPSYEDLLNKGLGAYLLPVQKD"
     gene            complement(27639..27782)
                     /locus_tag="PLS88_03400"
     CDS             complement(27639..27782)
                     /locus_tag="PLS88_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06427.1"
                     /translation="MFYIYLGILAIVLALTIWELFDTKKVSMQITAAMVIVPLLLRLL
                     MIK"
     gene            complement(27806..>28448)
                     /locus_tag="PLS88_03405"
     CDS             complement(27806..>28448)
                     /locus_tag="PLS88_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOU06428.1"
                     /translation="TLDFSELNKYGGLRLFSPLIFIFVLMILDKAVPRIFGLGMPLIF
                     ILGTALTWITGTRFNILKSAKKAIEDVLPVLGILVGVGMFIQVMTATGVRGFIVVNAL
                     SVPPALLYLMIAISIPLFGAISAFGSASVLGVPFMLALLGQDQITTAAALSLIAAIGD
                     FMPPTALAAIFAAKVVGLEKYGTVLKKLVVPALIIIAWAILFILFSKQISVLY"
BASE COUNT         7301 a         6532 c         6818 g         7697 t
ORIGIN      
        1 tgccaccaaa acatatgcca caagtagctg cgaaatcatt gagacagatc cactgctggg
       61 gacattgccg cctacaattc ccttaacaat aaggccaagg attaaccctc ctgtcaggta
      121 gccagaaggc agcttgagtt tgcttccgat ccaagcccct accagaattg cggtaacaat
      181 gattatcttt tctttcatag agcccccagc aacgctttta tttgattttt cttatcgagt
      241 ctatgatggt tccatcgcgg tactctacaa ctccgacaat ctcatcctga aagggcactt
      301 catcgggaat tcctgtaatg ctttcgatat cttctttaag cttatggatg tccttgatag
      361 gaatgccgga atcctttaat cgagccatga gatcttctcg ttttggatta acagaaatac
      421 ctctttcggt aacgatgaca tcgacggttt ctcctggcgt tacgaccgtc tgtactcttt
      481 cgcgtatgga agggacgcca ttgcgcatag aaggaattgt gatcagcgaa agctttgctc
      541 cggcggcagt atccgaatga cctccggatg ctccacgcag aatgccgtca tggcctgtca
      601 agacatcgac attgaaatcg acatcgacat ctagcgcagc gagcacgacg acatcgagat
      661 cgttgacaag acagccttta ttgaatgggt tggcgtacca tgaagagtct acctccatgt
      721 ggttcggatt gtgtaagatt gaatcggtga cagcagcatc gaagctctgt acgtcaaaga
      781 gtgtcttgaa gaggccatct ttgagcatat cggccatata gcccgttatt cctcctatgc
      841 aatagctgcc cttaatggag ttggccttca tgtaatcgcg catatagcgt gcaacagcta
      901 gacttggacc acctccgcca gtttggaacg agcagtcatt tttcaaatag cctgaggctt
      961 tgatgacctt aaatgcaagc tcggcgatat acaactgtaa gggatccttt gttattctag
     1021 tggcacctgt cgctatcttg gctggatcgc ctatggaatc gacaacgact acctgatcta
     1081 cgagatactg tggaatgctt attttagggg acagtggata atctacaaga ttgtcggtta
     1141 ttgcgatgac atgtcgagca tatctagcgt ccaccatggc ataacccaga gcaccgcatg
     1201 ctgtatttcc aattacgcca gtcatgtttc caagtcggtc gcatgccggc gcaccaagga
     1261 atgcaacatc gatcttgaga cttccttctt cgatggctcg cgctcgcccg ccatggcttc
     1321 gtattatcgc tgggctctct agaaggccat tcgaaatggc tttgccgacc tcgcctcgga
     1381 cgccagaggt gttcagcctt gtaactactc ctttttttat ggcatctgcg acacaatcgt
     1441 gtgagtcgcc cagtgaagag ggtgcaaggg tcagatccct aaagcccatt ttttcgagct
     1501 cggataagac aaggccgact attgcatcgc catttctgag atggtgatga aaggaaattg
     1561 tcatgccatt ctgcaaacca gagcgctcta ttgctgcctg taggtttgag gctactttat
     1621 tagtgctgat ggccggtatt cttcgagaga tcggagaggc tgtccacaaa aaggaaccgt
     1681 cgagtcttgc aaaagctcct gaaaatggtg tcgtagttcc tagacctggg atttctgaag
     1741 ggacaagccg gccgagtgag ttgcgcacac aatgatagtt catatttctg caacctccat
     1801 tccagcaagt cttgcaagtt cgatagttcg cagcgcctgc gacacaacag gggcatccac
     1861 catgcgccca tctaccgcta ttacgcctag acctctctgt tcggcttctc tagcggctat
     1921 aactatcttg attgcttttg caagctcttt tgtatccgga gtatagactg aatttatgac
     1981 agagacctgc gatgggtgta tagcggcttt tccgctaaat ccaagagcaa agcttagttc
     2041 tgtctgggct cgcagacctt cgagatcatt gacatcggta aaaaccgtat cgaaggcctg
     2101 cagcccatat gcttttgcgg cgataattac ggcgctcttc gcataaagta gctcattacc
     2161 ctcttttgtg gcgcgtatgc ctagatcggc tgctagatcc tgcccaccga gagtcaatcc
     2221 aacgagacgt tttgatgacg ctgcaatcga atttacattg actatcgcgc gtgcagtttc
     2281 gagcatggcc tgaatgccta ttttgccctt tggcagaccg tttatctctt cgagtcttga
     2341 tatcaattca tcagcggcga caacatcggc tgccgaatct accttgggca aacgtattgc
     2401 agctggactg gccgggacaa tctcttcaat atcttgctcg aaaaaaggag tgtctcgtcc
     2461 atttacgcgc actattatac ttatttcgcc aaaatcgaag ttacgaaggg catgttttac
     2521 aagtttgcgc gcagcatcct tttcggaaat cgaaatcgaa tcttcgagat caaagataac
     2581 agcatccgcg ccatagattg tacaattttg cagcatgctc gggtgattgc ccgggatgta
     2641 gagcagagaa cgcatgggat ggctttgaaa tacattcatc attttgcagc ttgtcttctc
     2701 ctttttacca aagcatatac aagtgataca ccagctaaca caataaatac agccgaaatt
     2761 ggtttctgaa agaaaattgc tggattcccg tcggagagga ccatggcatt cctaaaggat
     2821 tgctcagttg taccgcccag aattagggcc agcacaagag gcggaagcgg tacctccaga
     2881 attacgaaaa tatatccaac gagtgcggat atcatgacga gaatgagatc gaaggagcta
     2941 tagtttagga agtacgcgcc aataagcccc aatcccatca ctatcggcag cattattctc
     3001 tgcggtacac ggagaagttg aactaaagga atggctagcg gtaggttaat aaaaaggaga
     3061 atgatgtttc ctatgagcat ggaagcaagg acaccccaag cgatatccgg atgatccacg
     3121 aacaaagtcg gtccaggcct tacgccgagc agcataagag cgcccaacat gaccgccgtg
     3181 gtggcagaac ccggaatacc aagagtaaga agtggcacca tggctccaca ggaacaggca
     3241 ttgttggcag cttctggtgc agccaaccct tcaatcgttc cttttccaaa ttcttctgga
     3301 tgcttgctaa gattcttttc catggcatag gacatgaatg tggcgattgt accgcccatt
     3361 ccgggcagaa ccccgattac aaatccaaga ggcgtacttc ggagcatagg cgccaagcat
     3421 tgtttgaatt cttctttgct cactctcact tttcctatgc tctttgagtc gaagttgtat
     3481 accgtatcga ttgatttcaa atttttatag acttcggtaa cagcgtagac tccgatgatt
     3541 acaacaagaa aatcgatacc ttcgttaaga ctcgaaattc cataggtaaa gcgcacgact
     3601 ccagcgagtg ggtctattcc tatcgtcgag agcataagcc ctaccacgat tgaaaaaagt
     3661 cccttcaaaa gatttccttc agataatgta atggttgcca cgagggcaaa agccatgagc
     3721 gagaacattt ccgctggtcc aaaccttagt gctgcggaag caaccggctg gctcaagaag
     3781 atcataaaga tcatgccgat cattccgcca atgaaagaag cgattgcgga gatggcaagt
     3841 gcggtaccgg ctttgccttt cttggtcatt gggtagccat cgaagcatga gacaatagca
     3901 gaagcatctc caggtatgtt tagcatgatc gaggccctag aacctccgaa catggctcca
     3961 taatatacag ccgccatggt tattagggca gtggccgggt tcatgccaaa tgtaatggga
     4021 attagaatcg cgacgcccgt tgcaggcccc aagccaggta gcatacctat tatggtgccc
     4081 aatgtgcccc cgatgaaaac ccagagaata tttgtaaaag tgaaagctgt ctgaaggccg
     4141 atagatagat tcatcagtat tgaatgcata ggcttatctc ctagaatgga agcatcgttt
     4201 cgataatgcc acgaggaagg ctcagtttaa gggcaacccc gaatacaaag taggtcacca
     4261 cagaaaaaag aacagcgatc actatatttt ggacaagttt tcctctattt ataagcatcg
     4321 aaacgaagaa catgaacagg gcagtagcta tcatatagcc caatccatcg ataacgagcc
     4381 caaagataat gcccgcaatt gtcaaaaggc ctatttttat ccatacattt ttatgctctt
     4441 taaaaccgaa atctactttc ttcgagcgct tttctggatt tctatcctgc agaatcatgg
     4501 caatgccagt gattattgta actataccga cgaaatacgg gaaaatttta gggcccagcc
     4561 tgtctcccat cgtcatatga ggcaggcgca aggccgtaat ggaatacacc agacccaaaa
     4621 caatgctgat gattccagta agaatatttg tcgtcactta agtcctccgc ctcgaatttc
     4681 tgtcaaacaa aaaaggccgg tggacgcaaa tcttggcgtc gcaccggctc ttcatacttg
     4741 ctcgattatt tcttcaaacc tacggcagat aaaagttcat cgaatacctt tgtttgatcc
     4801 tcgaggaatt tggcatgcgt agatgagttc tgataatagt catcccaaag cagattctcg
     4861 agttgttcct tccaagtttt tgtttgtacc atcttcgtga acacctcgtc ccaataggct
     4921 acctgttcag gagttagacc aggagcgcca atgatacctc tccagtgagg aaatacaatg
     4981 tcgatacctt gttcctttat tgtcgctaca tcgggtagaa gttccagtct ctcgggcgag
     5041 ctcactccga ttactcgaag ttttttggct ttatgctgtt ctagcacttc tgcaaggcca
     5101 atgaccattg cctttacatg gcctccaagg agggctggaa cttcctcgcc gcctgcaccc
     5161 gggtatacta tgaattttac ttttgcgggg tctataccat agactcttgc gagctgtagg
     5221 aagacaagat gatcgtcgtt tccaagtgaa gggccgacag ctatgggtac agcgcttgga
     5281 tttgccttaa ttgcttccat gagatctttt actgttttcc aaggcgagtc gtcgcgaact
     5341 actatagctt cccactctgc ttgcatgcag gcaatcggcg taaagtcctt atatgtgaga
     5401 tcgcttgtgc ccaggagatt gtttagaatg agcaagctcg aggtcgcggc gaggtattcg
     5461 ccctggcctt tcttaccctt tagataagtc cagccgacag aaccgccacc gcccggcttg
     5521 tttgtgataa ttactggctt atcgacgagt ttttcctcgg tgagcgcttt ctgcattacg
     5581 cgacagagca tatcccatcc accgcctggg ttggctggag caataagctc gatagtcttc
     5641 gttggtttcc aagctggtgc agcaccaact gtcgcaacaa gtcccagaag aacgacagtc
     5701 agcacgaaca aagacagttt tttcatttag gtctcctcct tagaaatttg aaatatatga
     5761 tttaccgctg gggcaagccc gagtcggcta aatcttcaaa aaaattggaa atccttcagt
     5821 acttccgaat tacctgattt ttcgcgccaa atttggaagg attcgttaag atgatctgtc
     5881 catatctggc aagcaagatc aggatcatgc gcgaggaaag ccttatagag ttcatttctg
     5941 cggtggatac ttttgtctat gcggttggag aagaccatgc gcaagacgcg atagcggtcg
     6001 aaaatctctc tctcagacca tacagcttcg gtaagtctag atgacttgca agcttcgaag
     6061 gctatttcat tgaagcattt gatactaggc aagaggtttt cgagaagctc atcgcccgtc
     6121 atgctctgcc ttatttcata tatcttgtct aggacctctt tcatttcgac tagctcggct
     6181 tctgtgatac ggatggctga atatcttacg gcaagagtct ctagcgcctt gcgaattgca
     6241 taggagtgtg caagctcgta aggcgaaata ccaaggatac gatttccttt cttgggtaca
     6301 cgttcgatat agccgttgct ttcgagtttc tggagcgctt ctctagctgg agttctgctg
     6361 acaccgaact cgtgtgcaat atccgcatcg gtgaagtagc tatcaggctg caattccccc
     6421 aaaacaattg cttttctaag gcgttcatag atgacttcgc gcatcggttg gatgtgaagg
     6481 tcagggactt cttgtagaag gctcataata acttatatcc tcactaaaaa tttattgtat
     6541 tcggtattcc gtataccgta tacatgatgt ataatggtat aataagcgag gttttgaaat
     6601 ttgtaaagca aaatctttca gaaatcgaag ggaggatttt tctgtgggaa agaatatcgt
     6661 ccaaaagctt atcgacagtc atcgcatcga aggagtgcag aaatctgagt tcgaagatag
     6721 cgaagtgctt atcaagatag atcaaactct tactcaggat actactggta caatgactta
     6781 tctgcagttc gaggcactgg gtatagataa ggtgcgaacg gaactctcgg ttagctatgt
     6841 ggatcataat acgctacaag taggtttcga aaatgcagat gatcataatt tcctgcagga
     6901 tatagcatcg agatatggga tggttttttc taggcctggc aatggaatct gtcaccaggt
     6961 acatctggag cgttttggag ttcctggcaa atcgttattg ggctcagaca gccatactcc
     7021 cactgcagga gcgcttggaa tgcttgctat aggagctggg gggttagatg tagcacttgc
     7081 aatggccgga ttgcctttct ccattcccaa acctagagtt ctcaaggtgg agttaaaagg
     7141 caagctatct ccaatggtaa gcgcaaagga tatcgtgcta gaaatattga gaaggcttac
     7201 ggttaaaggt ggcgttggca gaatattgga atacgctgga gatggattgt cgacactgag
     7261 cattcctcag cgagggacaa tcgccaatat gggtgcggaa ctaggcgcga cgacttctgt
     7321 attcccgagc gatgaggtga caaagagctt cttggaagcc cagggaaggg caaaagattt
     7381 cttgccgcta ttcgccgatc ctgatgctac ctatgacgaa gaaattctta tcgacttaag
     7441 tagcctcgaa cctatgatag cgctgcctca catgcctgat aacgtggtac ccgtccgcga
     7501 agttgctggc ttacctgtgg atcagtgctt tatcggtagc tgtaccaatt catcttatta
     7561 tgaccttgct atgagcgctg ctattttaag aggccatcat gttgcgccca agacgagcct
     7621 tgtcgtggcg cccggttctc gccaggtatt gctgatgacg gcgagggatg ggatactgac
     7681 tagccttgtc gaagctggag ctaggattct ggaatgcgca tgtggccctt gcgttgggat
     7741 aggacaggcc ccggcaacga atgctgtatc tcttcgcacg agcaaccgca attttgaagg
     7801 gcgatccggg acaaaatcgg ctaggttgta ccttgcaagc gcggagacga cggctgcttc
     7861 agcaatcgcc ggagtcatta cagatcctcg aactctcgag tcgcatccaa ttgtggctat
     7921 gccagagagc tttcttaatg atgattcgat gttcattttc ccatcgaccg aaggggaaac
     7981 tgtaggaatt cggcgtggac ccaacatagc acccttgcct caatttgagg caattggaaa
     8041 tgaactaaaa gccgaagtat tgatgcttat gggcgacaat atcacaacag atcatatttt
     8101 gcccgctgga agcaaaatca tgtctttacg atctaatcta cctgctattt cggagtattg
     8161 cttttctgtg ctggatgcaa actttccagc aagagcaaga gccgctggaa aggggatcct
     8221 tatagcaggg caaaattatg gacagggctc ttcccgtgaa catgcagcgc tttcgcctcg
     8281 tttcctagga gttagtgcgg taatagcagt ctcctatgcg cgcatccaca gacaaaacct
     8341 tgtaaatgcc ggcattcttc ctttaatatt cgatagtccc gaagacatcg aaaatataag
     8401 ccagggcgat atactctgca tcaagaatat atatgaaggg ttgcgactgg atcgcttcga
     8461 ggtaaagaac gaaagcaaag gtgcggtttt catggctcgc catgggcttt cgcagcgaca
     8521 ggttgagatt atactagctg gcggagcgct caatgctgcg cgtcgaaagc tattggtcca
     8581 ttagtttggt aaatataatc gggtaatatc gggtaatata tgagcgagac attttttggg
     8641 ctcgaagaga gaattctcca tactaaagat gagcttgcac agcgagaagc acttttgacc
     8701 tctcgtgggc ttactgttcc agaaggagaa gagctggtcc tgggtctctt cgatggcgag
     8761 cagctagttg ccacaggcgc tcttgtccgt aatgtgctgc agggcatagc agtagatatg
     8821 agccatgagg gcgaaggagt agctgctgcc atagtatctt ctcttataaa aagagctgtc
     8881 gaccgaggga taacccaaat ttttctatac acggtaagcg aagatatctc tagattcgaa
     8941 aacttgggat tcagcaaggt agcagcggtt ccaaagggag cagcattact agagtggggt
     9001 tcggctggaa tcgaatccta cttgagctct ttgaaggcct tggccgaaga taagcccgat
     9061 catgccggag cagttgttat aaattgcaat ccatttacgc tcggccatag agcgcttata
     9121 gagtatgcat cctcgcatgt accctggctc tatgtgctgg tcgtagagga agatagatct
     9181 ctctttcctt ttagggtccg atacagacta gtacaagaag gtgtcgcaga tttacgaaat
     9241 ttgacggtaa tgaagggcgg accctatata atctcttcgg cgacatttcc aacttatttt
     9301 attcgtccag atgctaatga agcttcgcag agagcggtaa gaatgcatgc ttcgctcgat
     9361 gtcagcatct ttagaatgca tattgcaccc gcgcttaaag ttgccaatag atttgtcggg
     9421 acagagccat actgtcccac cacctcggta tataacgaaa taatgaagat tattctccag
     9481 gctagagaga ccgaggcttt tgggttcgat gcaaaaattc caccagtaac ggtacacgag
     9541 attcctcgct tcgaaatagg aggaactcct gtcagtgcct cgaaagtccg ggatatgatt
     9601 cgcgaaggga atttatcgga ggtacaaaaa ctagttccta aaaccacctg ggattggcta
     9661 aaaagcgatg aggcggtgcc agtaatggag agaattcgtc agagcgattc aaggcactga
     9721 taagtctttt atcacatttt tagagctaat gttttggagg agataatgag agtgcttaaa
     9781 tcagttactg cgggctcgct ggaatctagc gatgtgctcg tgacaataat gcctttgtcg
     9841 gaagaccgta tagagtacga catagaaagt atcgtcatta aacaatatgg gagaaggatc
     9901 cgaaaggtca ctgaggatat ggtagagatt gccaagatta caggagcacg cattcatgtt
     9961 caggatagag gagcacttga atgcactctt cgtgctcgcc tagagactgc gatcgaaaga
    10021 agtattgcgt gttgagcctg cagactgtgc tcgcggctcg tgagcttcgc tggaagaata
    10081 aaaagttgct tgcggaatcg ctggcacaaa aatttccagg gcatagatta gcccttgcat
    10141 tctgtactct aagaatgcct gcttctttaa ggatctctaa tctttacaaa gagattgtcg
    10201 tgcaattgcg agatgttctc gccggagctc tgatggatgg agctatccaa attctggatt
    10261 atgggcagga agaatacgaa gatgggccga gcgcttggct tgcctcggat tgcgatgaga
    10321 gaatcttgaa gcggatggct atcgccatag aggaagggca tgctctaggt tcgttagtgg
    10381 atatcgatgt gacagatgcg aaggccagac tcctttcgcg cagagatatg ggttatgcgc
    10441 ctagaaaatg ccttgtctgt gatgatgaag ctgcagtatg ttcagccggc cagcgtcact
    10501 cgctttccga aatcgaagca catatcgagg cgataatatc agactcgatg ggccaagaag
    10561 cttgggattc tcatgatggt cataaagagc gcgattgata ttgagcattg ggagaaatcg
    10621 atggatcggg acgaattgat tgcgcagata ggaaatgtcg ctttgactgc gacgctctat
    10681 gaagtctcgg cagcgccaaa gcctgggctt gtggatcctt tctcccgagg cgcacactcg
    10741 gatatggatt atttcacctt ccttgcgagc gcggtggcct tggcaccatt ttttgcagct
    10801 tttgccagca tgggtgcgga aattgagggc gaaagcccag cgctcctacc agaattgcga
    10861 aagcttggaa tcgaagccga aagggctatg tttgcggcta ccaatggagt caacacgcat
    10921 aaagggctca ttttttccat gggtctagta tgcgccgctg ccggaaggtt attgatttca
    10981 aaaaagcaag ctgaaagtaa cgataaaaac gctatgatat caggatgtgc ccaagaagct
    11041 gccgatattg ttgctggaat cacggttcgc gaactcgttc cacttgtgaa agcggacaag
    11101 tccctagtct ccgatccaat aaaagcaaca aatgctatgg tttgttctct cgatctcagc
    11161 gcaggcgaga aatcgtttgt caaatatgga ataagaggag ttcgcggtga agcggaagat
    11221 ggttttccat ctgtgataaa tcatgctttg ccaaggctcc attccgatct cttgaggggt
    11281 atgtcatgga acgatgccat gatcgatgcg ctcctagttc tttatctaat agtcgacgat
    11341 acaacagtac tgaatagagg agggtcggat ggtcttgcct atatcagggc gcaggcagca
    11401 gcggctcttt cacgcggagg aatgaggcta attgagggtc gagattttgt ccagagtctc
    11461 gatgcagact ttagtcagcg tggtcttagc cctggaggat gcgccgatct tctagcagtt
    11521 actgtgtttt tggagatgtt gtccaataaa tggactacac agcaaaaggc taatggatcg
    11581 gcgcaatgct cttcttaggc taaaaacagg atcaaaaggc tttatcgaca agtcggatta
    11641 aaaagctgga attacaccgc ctttgtaagt acttaggata aaatccttca ctttctgaga
    11701 cgtgagagct ttagcaagtg cttgaatacg gggatcattg gaatcgcctt ttctgaccac
    11761 aaggatattg gcatagggcg attctccacc ttcgaggaga agcgagtctt ttattgggtt
    11821 gaagcctgat tggagagcgt agttcccatt tatgaccgac cctgcaacat catcgagtgt
    11881 acgcggtagc tgcggcgcct caatttcctt aaatacaaat ctttttggat tttcggagat
    11941 gtctcggaca gtcgcctgaa gcccagcgtt tggcttaagt tttatgaggc ctttagactg
    12001 gaggaaaata agtgctctcc cttcatttgt tgggtcatta ggaattgcta tgattgcacc
    12061 attggggaag tcagatactt ttttgtattt cttggaataa aggccaagcg gttccacatg
    12121 gacggctact acgctttcaa gcgaaagttt tcgctccgca gcgaaggtct ctagataggg
    12181 cagatgctga aagaagttgg catcgagctg tttttcggct agcgctatgt tgggcgtgac
    12241 atagtcgctg aactcgatta cttccagctt tattccttgc gccgctaggt cgggtatgat
    12301 gaagttgagt atttcagcgt gcggtacgga agtggcacct actttgagtg taaccgaggg
    12361 agaggggcca gcaaaaacag acatgaccga aattaaaagg gataaaaaaa gcaatttgat
    12421 aattttgcgg ttcataagaa acctccagga taaaaaatta ttataagtcg ccatacggcg
    12481 tctctaagag agaccgaaag attcgagata tcgagttgtc gccaccaaga tctataagat
    12541 tggctcgggg atttgcctgg aggaaaagaa agactggatt tgtgatatgg atttacagga
    12601 tatttagaaa gaaggcatgg gtcgcggaga gcggcttcgc tgagccgctt cgcatgaaac
    12661 atacatgact attataagct ttcatcgcct tagcctttcg gagcaaaatg cattatttat
    12721 ccttctttgt caagcgggcc atacgagcaa ggcggccgta gcctcgatat gggctacggt
    12781 ttcctgctct attccgattt atgataaagg aaacacgaca cttcgtgccc gtcttctgca
    12841 tcctttagcg acggtcgcct ttccttgcat atatccatgc gcttggggca gcgatcatag
    12901 aaattacagc ctggccgctg tttgtcaggc gagggcacat ctccggtgag aattatgcgc
    12961 ttgcgcttag cctcgatctt tggatcagga atcggcgccg ccgacagaag agcctcagta
    13021 taaggatgta ggggtttctt atatagatcc gctgcgtccg ctatctccac aataagtccc
    13081 agatacatta cggctactct attggaaata tattttatta cagaaagatc atgagcaata
    13141 aaaagatagg ttaatccgaa ctcttcttgc aggtctttga aaagattgag aatctgagac
    13201 tgaatcgaga catcgagcgc cgatactggc tcatcgcata gaatgagttc gggattgagc
    13261 gctaatgcgc gagcaattcc aatgcgctgg cgctggccgc ctgagaattc atgcggatag
    13321 cgatttttga aaaagcggga tagccccact ttttccataa gctcctctac ccgattgtct
    13381 cgatcttgag tactcatatc tatgagccct tgcttttgga atatgcgaat gggctctgca
    13441 attatatcgc cagcggtcat acgtggattg agcgaagcat aggggtcttg gaaaaccatc
    13501 tgcatatttt ttcgcgcttc catcatttcg ttgggcgata gcttagtgag atctttgcct
    13561 ttcagaatga cctcaccggc agtgggctta tagagctgat ctatggcgcg agccgtagtc
    13621 gattttccac aaccagattc tcctacaagg ccaagagtct cgcctttttt caccttaaaa
    13681 tcgatgccgt ccacagcata gatatagttc ttagtctttg agaacatgct tgttttgaca
    13741 gggaaatgca tcttgaggcc gcgcacatct agaagtactt cgtcgttttc aggcattctg
    13801 aatctccttg ttgtagagcc agcaagctac gctctgattg ttctcaaagg aaacgataga
    13861 tggatatttc tctctgcaga tatccatagc ttgttcgcag cgcggataaa atgggcagca
    13921 gtccggcaga tctatgacat ttggcggctg gcccggtatg gaatataggc gcaccttggt
    13981 ctcttgatcg agccgcggta ccgagcggat taatcccatt gtgtaaggat gcttaggcga
    14041 ttcgaagata tcttcggtgc ggccgcgctc gacgattctc cccgcataca taacgcagat
    14101 cgtgtcgcac atgccagcaa cgacacctag cgaatgcgta atgagaatca ctgcggttcc
    14161 aaattttctt gtgagttctt tgataagctc gaggatttga gcctggatcg taacatcgag
    14221 tgcgctggtc ggctcatcgg caatcaatat ctcgggattg caggatagtg ccatggcaat
    14281 cattacgcgc tggcgcattc cgcccgaaaa ttgatgcgga tattggtcga tgcgcttttc
    14341 cggagcaggg ataccggcaa atttcagcat ctcgatggct ttttctttag ctgctttttt
    14401 atctagccct tggtgcaact gtatcgtctc aatcatctgt gtggaaatac gcaagaaagg
    14461 gttgagcgat gtcatagggt cctgaaagat catcgaaatc ttatttcccc ttatatggcg
    14521 aatttcgctg ggcggcaact tgagcaaatc tgtgccatga aaaagaacct cgcccccgac
    14581 gatttttccg ggaggtgttg ggatcagatt gatgatcgat agattggtta cacttttccc
    14641 cgatccagat tcacctacaa tcccgagggt ttcgctgcgg tgaagctcga agctcacgcc
    14701 atcgactgcc ttcaccactc cctcatctac attaaagtat gttttcaagt cttttacttg
    14761 aaggatgact tcgtcagaca tttaggctcc ttatcaagtg cggttcttac tctgcggatc
    14821 gagagcatct cgaagaccat cgcctaggaa gttcatgcag aagaggaaga tcgtcattgc
    14881 gatacctggg caaagcagtt gccaggcata ggattccatg gcacggactc cttccgagac
    14941 gagtgtgccc caggatgcat caggtgccga cacaccgagt cctaaaaagc ttagaaagga
    15001 ctcattcatg atgaaacttg gcatcatcag tgtggaaaat acaatgatga ctccgagagt
    15061 attgggtaga agatggcgga aaattatgcg ctgagtactt gcgcccatag accgcgctgc
    15121 ttcgacaaat tccgaattct ttaggcttat gatctggcct cgtaccacgc gagcaatagt
    15181 tagccaggag accaatgcta ttgcgacaaa gagcacaaca aagcttccgc gtgctttctc
    15241 gccaatcata gccattataa ttatgacaat gagcatatag ggcagcccat acatgatatc
    15301 gacaaaacgc ataagaaaat tatctagcca accgccgaca tagccggaaa ttgctccgac
    15361 tagaatacct accaaggcag ccgtcagcgt gcctacgatt cctatagcaa tcgaaatgcg
    15421 accgccatag attatgcgtg caaacatatc gcggcccaga ttatcggttc caagaatgta
    15481 tacgattttg tagtcgggat ttgtatcgag ttcggctttg actctttcca gggtttcttt
    15541 ttgactgatg agctcgcgga ctactggatc ggttgtattg ccagagaatc cttcgccctc
    15601 ataggtaaaa gagaatcccc cttcttgact ctcagtataa ccatcgctag gttcgctgct
    15661 ttcctcttga gtatccagcg atccaaaatc gaagaaggaa ctgaaggcgg actgctgttg
    15721 ttgttgctcc tcgacccttt tctcgagatc cacgatacgg ttctcgattt tcttgacaac
    15781 caattctcca gctggcttga aagagggagg taagctcgca ttcgtaatga cttgtttgcg
    15841 gtaatctatg agaattcccg tacttgtaag aagaggcgca aaaagactga taactacata
    15901 tatacttact attactagac ttatcaaggc catcttgttt ttgcgaagtc gcttccatgc
    15961 atcagcccat agagagacag gcttcattac ctggttgaat tcgttgactg cattttcaag
    16021 ttgttttttt gccatctctc accccttact tgtaggaaat tcttggatcg aggaagccgt
    16081 aaagaatgtc gaccacaaga ttcgcaatga tgagaataat ggaatagaca accacttcag
    16141 ccattatgag cgtataatcc cgattcaaag ccgattgaac aaaggggcgc ccgattccag
    16201 gtacaaggaa gaccgattct acgactatcg agcctactac aatgcccgaa aaggctgggc
    16261 caagataggt gactacggga agcaaagctc ccttaagaac gtgtttccac actacaaccg
    16321 attctttaag cccttttgct cttgccgtgc ggatgtaatc cgaacgcagc acttcgagga
    16381 tgcttgcgcg tgaaagcctg gcgatatagg caaaatatgg aaaagataga gttattgctg
    16441 gtagtattac cgcctttatg ccatgagtcg agatccactg acctatgggt aaaatcttga
    16501 gcctcatgcc aaagacgagc tgcaagacag gccccatcac aaatagcggc acagaaatgc
    16561 ctatgacagc tatcgaagtg acaacgtagt cctgccattt attttgcttt agtgcggaaa
    16621 tgatacctac cgcaattccg ccgactgtcg caagcaccaa agagataaca cctaacgaca
    16681 tcgagatggg aagaccttcg tcgataatct cattgacggt tagatccctg tatcgatagg
    16741 aagggccgaa atcccagcga attatgtcgc ccatataccg taaatactgc ttgatgagag
    16801 gttcgtccat atgatagcgc ttcatgaggt tttgaaggat tgcctctggc acttcgcgtt
    16861 cgcgagcaaa agggcctcca ggtgcaagtc tgatcagaaa aaaactcagt gtgatcacga
    16921 taaagagcgt aggaattatg ctcaatactc tccgaatgaa atatcgtggc attcggaatt
    16981 actcctttcc gaggcgcaaa ttaattattc aaaaagaata tatttgcgta attggaatct
    17041 cgaagcatga gaagctattt ctcaagcaaa aaatattata ctccaatcct gaaattttat
    17101 ctacccaaaa tttcttaatg ttgtgagctg caaaaaataa tctcaagata aaaaaggggc
    17161 tgcccatata ggacagccct ggtttcttac attaacttat ttttattttt tgggtcggat
    17221 aaatttccaa ggatggatat tgagtgggtt aggataccag ccatcccact tgtccaaatc
    17281 gattaggttc tgtgttacat agaagtatag cggaagaacg gccatatcct ggtcgataag
    17341 gattttctcc gcatccatca ggtactggaa acgttcggtg ccgctcgtaa cacgagcttt
    17401 gttgagcagt tcgtcgtact tcgggttgga atagagtcca tcgttctggg taccgccagt
    17461 gatgaacatt tctaggaagg tcgatggatc gagatagtct ccaatccaac cggcgcgagc
    17521 gatatcgaag ttatgcgact gggatcgagt gtcaaggtat gtgccccact cctggttcat
    17581 taggctgaca ttgataccta gattcacttt ccactgggct tggacgaatt ccgaaatatt
    17641 cttgtgagcc gaagaagtgt tatagaggat tgtgaatgcc gggaagccct ttccatctgg
    17701 atagcctgct tccgcaagaa gcttgcgtgc tgcagcagga tcgaagtcat agccctttgc
    17761 cggagtatag ccagtgctag gaggcactag gctatttgcg ggtatctgtc cgccgcgggt
    17821 aaccttattg acaaggtcgt ttttattgat agccatggcg agcgctttgc ggacgcgagg
    17881 atcatcgagc ggctttcggg ttgtgtttag gatataatag taagttccat attgcggagc
    17941 gacatggaag tccttgcgca gctttacttc atccatgagc tcaagtggta cggcctctgt
    18001 ccagtcagcg gctccactct tgtagagatt atagccaaca ttgatgtcgt cgttggcaat
    18061 gaaggtaatc ttctttaggg cgacattctt tgcatcccaa tacttatcgt tctttacaac
    18121 gacaaccttt tcctggggct tccattcgga gaggacatag ggtccgttcg ataccatctt
    18181 gccaggcttt acccaatcct taccatattt ttcgatggca tgcattggaa caatggcgaa
    18241 tgcatagtga ctgaccatat ctgcaaagaa tggaattggg ccaaccaatt tgacttcgaa
    18301 ggtgtattta tccacagctc gaatttttag gctctctgga ccagctttgc cttcgagata
    18361 atccatgccg ccttcgatat acatagcggg cagatcggca tactggaagg cattcttcgg
    18421 atccatcttg cgcacccagg actggacaac cgtatcggcg gttatttcca caccatccga
    18481 ccatgtaacg cctttgcgga tcttgaaggt gcaggtcttg taatccttgc tgaacgacca
    18541 gctctctgca actgcaggga gtgcacggtt ggtgcgcgga tcattgattg tcagaccctc
    18601 gaaaagcgcc acatagatgc ggtgctcagg cacaccttcg accgcatgcg gatctagcga
    18661 agtaggctca gtaccattta cgatggtaaa gtcctgagcg ctaaggccta aaaccgcgac
    18721 tagggcgatc aacgcgaaaa ggatcttttt catgttacct cctctttaag agacattctc
    18781 aaatactata tactcctttt atgagcaatg caagaatttt tttggatttt agccataaag
    18841 ctcaacaatt tgtggatttt taaggcataa ttgagaatta tcgttccgat gcaagcttct
    18901 atcatagccg agctctctgc tgaaaacata gaaagaaagc ttgcacattg ataattgact
    18961 tcatgggata aagaattcca tagaataaaa actattagga gatgcgctat gtacgaattc
    19021 tatgcaccga ccaagatatt gtttggggat ggctgtcttg ccgaagctgc atcgcaactc
    19081 aaagaaatga attcgaccaa ggtgctgatt gtgaccggta gatcgaaaac tgcttcttcg
    19141 caaggtttta aggacctatg cctcggctta gattcgagtg gcatacacta tgttcatttt
    19201 ggagaagtcg gcgcagatcc cgacactgct attgtagata aagggacgga ggtttataaa
    19261 gcgcagcgct gtaatgccat tatcggtttt ggcggcggta gccccatcga ttgtgcaaag
    19321 ggtatcgctg cgagcgttgc cgaagatcgt cctatccgcg attttgtggg aacgggattg
    19381 gcatttacaa agcctgtacc tccacttgtc gcaattccaa cgacagccgg tacggggaca
    19441 gaggttacaa atgcggcggt gttcacggta gtggacgaaa gtggtcatcg aagcaaaaaa
    19501 ggcacttcga gccagtatta tttccccagg cttgctattg tggatcctgt tttacatatc
    19561 agcatgccac cgccccttac cgccgcaacg ggcatggatg ctcttaccca tgcgatcgaa
    19621 gcttacgtga gctgttttag cacgcctatc agcgatatgt actgcctcga atccattcgt
    19681 cttattggtc gtagcctaag ggcggcgtgc cacatctcca aaggagctga agacgaagac
    19741 atacaatcga ggcgcgacat ggcgctcgca gcgaccttgg caggagtggc tctatctcag
    19801 gcaggattgg gaatggtgca tggctttgca catcccgtag gagctttggc tggacttccg
    19861 catgggcttg ccaatgcaat aatccttcct tttgtgatgc aatccttgat tccagatgca
    19921 gcagagcgcc tttcggatat tgggaaagcg cttacggcta aggacaatat cagcccggaa
    19981 gaatcggtgc gcgcaatctg ccaactgggc catgatatag gtatccctga gaaccttgaa
    20041 gctgctggtg tacctccagt ctatttcgac gatattctta gcgatgctct ttcttatcga
    20101 aggcgtaagg ctagtccacg ggcttttgtc gatgcggagc tcagagaatt gctcgagctt
    20161 atataccatg gtgttcctca gcaatgaaga gggcctctag tcttgctcga actcaaggca
    20221 agagccgccc acagcttttt ggaccacaat tctcgatggt attcggctat agagctcgtc
    20281 cacatgggag atgatagcaa tgctcttgtc acctctaagc tcttccagga ccgagatggc
    20341 cagcgaaagc gactcctcat cgagacttcc aaagccttcg tcgataaaga gcgattcgat
    20401 gcgatttgct tttgctcttt cttggatcga atcggctagt cccagagcaa ggcttatgga
    20461 aagcatgaat ttttctccac cggagagcgt gcctacaggg cgacggcccc cattccaggc
    20521 atcgagcaca tagagttcca ggcctatttt ttggttaccg ctgccaccca gaatatcggc
    20581 ctccaccaag aaacggccat tgctcatcct gtagagtcgc tcggaagctc gcatggctat
    20641 ttcgcggaat tgaaggccca gcacatagtt cttgaacggc atttttctct gtggatttag
    20701 gcttcctccg agcaatttgg ccagtttttc ctgttttccg aattcctcaa cagcactatc
    20761 atattctcga gtcacttctt ctcgtctgga aagaaggcga cgaagctgat cgagttctga
    20821 tatgaaatta gcacgcttgt cgcgtaaatc cgatatcgcc aattcaatat ttttctttcc
    20881 ctcaaataga gtgtcgagct cctctctaag ggctttttct tgttgttcga gataaagggg
    20941 ctcgagcatc tccttatgtc tagactgctc cccattttct ttctcgcgtt cgagcgatcg
    21001 atctgatcga tctactgcta gatttggagc ttgattgagt agagtcgagt tgaatgaatt
    21061 gaatgagttg aatggggaca gagcctgcga ccgtgcagcg agcacttcat aggatgcacg
    21121 agcttcaata taggattgtc ggaatgccga aatctccctt gcctcttctt taatgcgatc
    21181 gcttgtccag acagaacaaa ggacttcttc gataagttct tttaatgctg gctcatttat
    21241 tgtatgtgcg aaaaagatac tctccaaagt tcctgaaagc tcgataatcc gctcagagct
    21301 cagtttgagt cttttaattt ttaaaaagac attgtctagc ttgggaaaga gctcttttag
    21361 cgatcttgaa gacaatcttg agggagattc tagcgaaccc gagaaatcat taggtgaacc
    21421 tagagggcct tgtaattgcc agcttgtctg ctcttcattt attggtgcct ctttctccga
    21481 agaaattacc gaagattcaa gaagccgtaa gagcctcctt gcttctattt tctcggcttc
    21541 atggagaaat tcctcgagcg ttatagcaag agccgatcct ttttgcgtaa ttgtctccaa
    21601 ctgcatgtcg attttgatgt ttgcagagct ccaagatatt tcccattttt gcgctcgctc
    21661 ttctatttgc tcgatctcgt tgtggagatc ggcaagatta tcttccacct ctttgaggtg
    21721 aatcctagga tcctcttttc cgatctgtgc ctcaagagaa gcaatatggg catctaattc
    21781 ggcgcgctta atctcgagac cggtcttttc ctcttgaatt cgaataaaat cgctctctac
    21841 ctgatcaaga gtctttcgca gtttttgcaa tgtttcgcgc tcatgttgat agtatttcag
    21901 tcgtccttcg agttcgcgac gctgagattg aaaatgtgag ccagtcaatt caatcatcga
    21961 agaagaaatc tgttcaaaag ctatattggc ttcatctcca aggaattcgc agggaatgga
    22021 gttccattct tccgagattc tattgattgt attcttaaga tcttgtgtga attcctcgat
    22081 gcggttttcc agattctctt tattttgaat taaggcggca cgagatgatt tgatctcttc
    22141 cactactttt tgtgtggctt taatggtttt gtcatctaga gctttttgct caagcttagc
    22201 tggattggga tgatctcgag ctccacagac aggacatggt tcaccaggaa tgagcgaagc
    22261 ggcaaggata ccagcctgag catgacgaat ttcctcatcg attcggtctc gattctgttc
    22321 ggcaatagcg agctcttggt ctatggataa aatagaatct tccgtttgct gaattttacg
    22381 taaggtttcg gagcgctgtg cttctatgtt ttctttctgc tctatccaag tctttattcg
    22441 ctttaatgct tcgtccgcga tgataatctt ttgaaatgat tcctgcgcca tgggctcgcc
    22501 ttgtagggcg ttttcaagct ctgttatgct tgacttgagc ctggcgttct ctttttgtcg
    22561 agattcccaa tgctccgaag cggcatcgag tctttgcaga acctctctac attcgccttc
    22621 gcattgttcc ttttggttcc atgcattaac tgcactttga tacaatgcag ccctgcgctg
    22681 cagattgtcg taatcctttc gaagaaaggg caatgctttc tttttctcct ttatctcgat
    22741 ttgctctgtt tcgagcattg aaagctctgt ccccgtctgt aaagcttcta aaagaatctc
    22801 gattagttcc tcgtaatcag ccgatactcg tctcgccaag ctcgctagtt ctccaagtga
    22861 tttggctgcc tcgagctttc tttcgcgttc atcgaagata ggctttaatt cttctttttc
    22921 atggaagctt ttacaggccg caagcataga ttgcaggaat gaaaggcgct cgtttttttg
    22981 agcgatttga gtttctaaat ttaatgcatt tcttgtcagt tcttctattt tttcatcgaa
    23041 ttcggagatt gcttttcccg attcttttat acgagattct ggctcgatgc caagctcatt
    23101 agagatgcgc tcgatttcct cttttttagc tttgaggtaa gcatcgagct ccatgacttt
    23161 ttttcttgcc gtctcggtga ttgtatcgta gattcccaca tcgaatagtt tttcgagaat
    23221 ttcgacgcgt tcagtagttt tcatttcgag aaatttctga aactctccct ggggcagaag
    23281 tacgattttg gaaaactcct cggcgctaag gcgcaaaaga tcgatcaaga tctggtttgc
    23341 ctccgtaacg cggtcggcta gtattgcata actccccttt tcattcattc gatagagcgt
    23401 aacctcgctt gcagcctcag attcctgtcc tcttctattg agtttcttgt aaggcaagct
    23461 tcgatatact ttccattgtg cagatgaagc taggaactcg aactggaccc agggcttgct
    23521 tccagcagga gaaaagcgcg aaccaagttc acgctcgaga ttgctccgtc ccccgacggc
    23581 cttgccatag agtgcataag caatagcatc gaaaatggtg gttttccccg aacctgtctt
    23641 gccgtagata aggaaaaagt cgtcgagctt acaaaaatcg atgtcttgac gtttgcagta
    23701 tggaccaaag tgttcgaaag aaagggaaag cggcttcatg aaagctcctc ttcggcattt
    23761 ctaaggagaa cctcgaaagc cttcagtaat tcttgagaag gcggctcatc gagaatgcgt
    23821 tgcgtaaagc ttatgaagtc attgagaact gtagtgtgaa gtgatttgcc ggcaaggttt
    23881 cctgtatcat ccagacttga tgtctctcca tttggaggca taagagtagc tatatctccg
    23941 actttttcaa cctctgtccc cattcttttt tcaaaggcct cttggcgcac atacatcaga
    24001 ttgggaaagc gaagagcaag acgattaaaa gcgtcatata cgggctcttc atctgtaagg
    24061 agcacttcga catagtcatc atctataaaa gcagaatcgg ccataagttc agcaaagcta
    24121 gctctaacgc gcttaaagac atgcaatggt tttagttcta ctcgctctat tcttgccggc
    24181 tcgccatcct cgagttcgac atataaaaat cctttttcga taccttcatc tccaaaggac
    24241 attggcatag gagcgccaga ataccaaact tttggggaca gagcttgcat cttgtgcaag
    24301 tgcccaagcg ccacataatc gaaggcctcg aatagagaga cgggcacggc ttctgtccca
    24361 ccgataaagg ctatatcgga ttccgaaaga actgctcccc taacatagca gtgggccaca
    24421 aggatattca ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttgcaa
    24541 tagagggacg gaggttcgct ataatactct ccatgcgact cgccatcgag cttatggtct
    24601 cgcttatggt ctctgtgcag cctgcttcta cttgcccctc ttcggccaat cttctcttga
    24661 attcgctgta agttccatga gtcacaaagg gcagtgccca aactgcagcc ctctttccac
    24721 ccttttcaag cactagggtc gggcttaaaa gaatttcgct aactaggtgt atgcctacgg
    24781 attcgaacaa ttctgcagca aaagcgattc gttttggcga atcatgattt cctggaatga
    24841 cgagaatgcg cccattctgc ggtagcgcgc gctttagctc cgccataaac cagccgaaca
    24901 tagtctgagc actctctgaa ggaatgcttc gatcgaacac atcacctgca atgacgacaa
    24961 gatcgagctt gagcgctctt atcttttcga caatctgcgt tagaattgcg gcttgatcct
    25021 gaaccaaatc ttgagatcga aggatttttc cgagatggag atcggaaata tgagcaatct
    25081 tgaatatttt gtcagaatgt gacatctgga atcttccctt tacaagctca gtttagtatg
    25141 tttttaatgt ggactcaatt cggagcaggg aataaaagcg ggattaactg gccgtctgct
    25201 ctctgctggc gtcaagaatt tttcagcgtt tcaatttaag ctagtatgac gtgatgttat
    25261 tctgctgatt attgttctag acgcctccgt aaaagacgat tccgcaaagg agttattcca
    25321 tgatttatcg acatttaggt tcagcaggta tcaaggtttc agtgatttcg ctgggttcgt
    25381 gggtgactta tggctcgcag gtcgatatcg acacagcagc acagatgatc aaatatgctt
    25441 acgaccatgg tgtcaactat ttcgataatg cagaatccta cgctggtggg caatccgaag
    25501 tcatcatggg tcaagccttt cgccggcttg gtttgaggcg aggctcctat cttgtttcga
    25561 ccaaactttt ttgggggatc cacgaaggtc ctaacgaaaa gaatactctc aaccgcaaat
    25621 accttctcga ggcaatcgaa ggcagtctag agcgtctggg aatgaaatat gtcgacttct
    25681 tgttctgtca tcgtccagat ccagaaacgc caatcgaaga gacagtctgg gctatgcacg
    25741 atatcatatc ctcgggcaaa gcactgtact ggggaacaag cgaatggtct gcggagcaga
    25801 ttcgcgaagc atgggaaatc gcggacaagt gcaaccttcg aaaaccgcag atggagcaac
    25861 cccaatacaa catgctggtc cgcaccaaag tcgaaaaaga gtttgcacga ctttatcagg
    25921 gcataggtct tgggcttact actttcagcc ctcttgcttc tggccttttg tcaggtaagt
    25981 acaaggatgg tattcccaag gattcgcgct ttgcgttgcc tggttatgag tggcttcaag
    26041 agcgttggta tcagcccgaa ataatagcta gcattgaacg gcttcgccct atcgccgaat
    26101 ccctcggtgc caccatgagc cagcttggcc tagcttgggc tgcacacaat cccaatgtta
    26161 gtaccgttat aacaggcgca agccgtctat ctcagctcga agaaaatctc ggcgctatcg
    26221 agattctgcc cagactcaac actgaaatcc tagacaagat cgatgaggcg ctggaaccta
    26281 ttctggtgat gagaaattag caaagagaat tcgagaattc agcaatatag aggtttgact
    26341 cgccattttg tctggtgctg tgggttttta gtgccgcgag ctatgtggcc ccagattatc
    26401 tagggccaca attaaaaagc gcgcaaccct aaagcatagc ctccataaaa atctcccttc
    26461 tcaaaagcgc gccgcctagc ctagttgttt ctcaatcttt ctgcacaggc agcagataag
    26521 caccaagccc tttgttgagc aaatcctcat agctgggaat attgtcgaat tccaatggtg
    26581 tttctgggtg ctcccaattc caggagttga tgatctcggc aatcgcagta acatgcctgc
    26641 caactcgttc ttctatagga tggccattct catcgtatgg aacatatagg cgccctaatt
    26701 ttgccgctcg cacataccat ttgtccttgc cggtgagcac aagagcttcg tctgtacgcc
    26761 cacgcaatcg tccttgtgaa gcgtttgcgg tctccatcaa gagaggcaga gtgtccgtga
    26821 agtctcctag ttcgcggtgt gtaagtccgc gaagattgac tggcgagggc tcgagactga
    26881 tctttatatc ctgcatttcg aggccaagca cagtatcgga tgctagctgc atggcttttg
    26941 ggtgtgcgac gattgcgttg ataaccggat attctggaga cgcttcgtgc aggtccactg
    27001 ttatggtgac attctgttct ttgataagtt gaactattcc atagcagact ttttcggtta
    27061 gtgtaccatc ggctcggcct ggataaccac ggttcaggtt gcgagtctcg ctcccagaga
    27121 gtgtctgacc agacgaagca tgtatataga cgttcgggtc tggccattgg tgtacggggt
    27181 tggttgctct cgatccataa ctgaagactc gcgggccatt tggcgtttcg atggtaaagc
    27241 gctgtggagc gccttcctgt ggatcatttg ccgtaaagcc cgaggcattt gctcgcggga
    27301 taacgtagag tgtgcctgtt ctaggcaaga agttttcgat aagcaatatg gcagcgagat
    27361 ggccagctgg ctcgttgggg tgagtgccgc caagcaccaa catagagact ccatcatcgc
    27421 ttcctttcat aatgtagact tctgtatcgc cggcgctgcc tttcaaagcc ggaaaccagt
    27481 ccgaaagcat ctttatttcc gtaacaccgg gtccgctcac aatcttatcg ggctttcgca
    27541 tcgccaaaaa agaggaacct gcaagcgctg atgccacaat agctccggca aggcatataa
    27601 ttgccgttat tgtatgttta tgcatgaata tccctcactc actttatcat cagaagcctt
    27661 aagagaagcg gcacgatcac catcgctgcg gtaatctgca tagagacctt ctttgtatcg
    27721 aagagctccc atatggttag agccagcact atagccagaa ttccaagata tatatagaac
    27781 atggacacct tccttacaat tcttactaat aaagaacgct gatctgcttc gagaacaaga
    27841 tgaaaagtat agcccaggct atgataatca gcgcagggac taccagtttt ttcaaaaccg
    27901 ttccatactt ctcgaggcct acaactttgg ctgcaaaaat ggctgcgagt gccgttggag
    27961 gcatgaaatc gccaatagca gctataagcg aaagcgctgc agccgtagtg atctggtctt
    28021 ggccaagcaa tgccaacata aaaggaactc caagcacgct agcggaaccg aatgcgctga
    28081 tggcgccaaa tagcggaata gagatagcga tcatcagata tagaagtgca ggggggacag
    28141 acaatgcatt tacaacaata aatccgcgca cacccgtggc tgtcattact tgaatgaaca
    28201 tgccgacacc tacgagaatc cccagaaccg ggagcacatc ttcgatcgct ttctttgctg
    28261 atttcaagat attgaagcgc gtgcctgtaa tccatgttag cgccgttccc aagatgaaaa
    28321 taagcggcat tcctaaaccg aatatacgcg gcacggcttt atctagaatc atcaatacaa
    28381 agataaaaat gaggggagaa aatagcctga ggccgccata tttattcagc tcgctgaaat
    28441 cgagggtt
//
LOCUS       DAOSOB010000019        28056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1702_length_28056_cov_65.990536, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000019 DAOSOB010000000
VERSION     DAOSOB010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 28056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28056
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1702_length_28056_cov_65.990536"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..81)
                     /locus_tag="PLS88_03410"
     tRNA            complement(8..81)
                     /locus_tag="PLS88_03410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Arg,seq:ccg)
     gene            234..914
                     /locus_tag="PLS88_03415"
     CDS             234..914
                     /locus_tag="PLS88_03415"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOU06429.1"
                     /translation="MYGEPILLASESSRRGSILESIRIPFIRVMPSIDETAFDDLDPG
                     ERVIALATTKAKRGNSIWFEKTQRERVVKSSIEKYLEKGSFDRECLREPRFVLGADTL
                     VAFKISEGWITLGKPANKLEAFEMLSMEAGKRQTVFSGLCLLDMASNRYYPALSISEV
                     KFAPMTPEEISWYLCFEEWSGAAGAYRIQEIGSCFIEEIRGSSSGVMGLPIHELYGIL
                     RESGYLDP"
     gene            1046..1927
                     /gene="rpsB"
                     /locus_tag="PLS88_03420"
     CDS             1046..1927
                     /gene="rpsB"
                     /locus_tag="PLS88_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOU06430.1"
                     /translation="MAVVTMKNLLESGVHFGHQVKRWDPRMKKYIFAERNGIHIIDLQ
                     KTIQAIKEAYDAVQKVASSGKSVLFVGTKKQAQAAIQKEAERCEQFYVNNRWLGGMLT
                     NFSTIRKSVQRLKKIERMEVDGTFNSLTKKEIASLQKEKAKLEKNLGGIKEMTSLPGI
                     LFVVDTRKEAIAVAEAQRLGIPIVAIVDTNCNPEGITYPIPGNDDAIRSISLFTQIIA
                     NAVIEAGASEGLKIIETLPPEEEQELVIPQKLEEDEVEIDLDNYAESSSQSNKETDEQ
                     QEVEQPIIDEDRLYDEN"
     gene            1949..2800
                     /gene="tsf"
                     /locus_tag="PLS88_03425"
     CDS             1949..2800
                     /gene="tsf"
                     /locus_tag="PLS88_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOU06431.1"
                     /translation="MEIKANDVKALREKTGAGMMDCKKALIEANGDFSAAEKLLREWG
                     IAGIEKRAGRATNEGRIFIAQNAKELELIELACETDFVARNKDFIAAGTKIAERALSQ
                     KASGTNESLETIVKEIASLIKENIALRRVVHFYAEENDILHAYLHGEGRIGVVVKFRA
                     NNPKAFENEKITAFIHDIALHVAAFNPMFLDESKVSTDWIAEQKEIFQKQLELDEKLK
                     SKPEKVLEGILSGKLKKLLAEVCLLNQGFVRDEKSSVAAVLAQVSKETGYQLEIIDYY
                     FAKVGQD"
     gene            2889..3443
                     /gene="frr"
                     /locus_tag="PLS88_03430"
     CDS             2889..3443
                     /gene="frr"
                     /locus_tag="PLS88_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOU06432.1"
                     /translation="MESNKAAHEEKMKKTIAAFQDELNSIRTGRASSVLLDKVRVDYY
                     GQKMPLSQVATISVPEARLIVIQPWDRSLFSEIEKAILKSDLGLNPSNDGKVLRIAIP
                     PLTEQRRKELVKTARSIAEQARVAIRNIRRDALEELKKQQNAGGVAEDALKKEEAELQ
                     KMTDDYIAQIARILEMKEREIMEV"
     gene            3456..4160
                     /gene="uppS"
                     /locus_tag="PLS88_03435"
     CDS             3456..4160
                     /gene="uppS"
                     /locus_tag="PLS88_03435"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOU06433.1"
                     /translation="MKENCMPSHVAIIMDGNGRWATQRGLSRTEGHKEGLETAKRIVL
                     ASHKEGIKFLSLFVFSTENWKRTEQEVGFLMSLIRVHITRELDFYRENEIRIVHSGER
                     SGLPHDVLSEIDKAIENTAHYRSLTVNLAINYGGRDEIVRAMQRILASQANGDKKPID
                     ELTVMQYLDHPDIPDPDLIIRTGGEMRLSNFLLWQSAYSELYFSPKYWPDFTPGDLHA
                     ALVDYMHRERRFGDAR"
     gene            4157..5038
                     /locus_tag="PLS88_03440"
     CDS             4157..5038
                     /locus_tag="PLS88_03440"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOU06434.1"
                     /translation="MNEKNTRARLLLFFIGIPLLILSLFFLQFAHYIIISGIIFVVQF
                     LSSKELRNMLTRAAMPDSGDSLTFIGLIPSVLTYISCFLSGSPLETAFVLFIAWSFCL
                     ILILVPLAFQKKNSFPELIKQAASIVLVHFYTALLPGFLIFIVSGFPNSQYTIITFAM
                     LTFGNDSLAWLFGKYLAKKRNLLDVSPNKSVAGFIGGTIGSIIAAFIGLGPMAGTWKP
                     SGASYTLLSLSLGLGMAFFVIVGDLFESALKRSARMKDSGSIVPGRGGILDSFDSLYF
                     SAPFFVAFSFSFHLFNL"
     gene            5075..6202
                     /gene="dxr"
                     /locus_tag="PLS88_03445"
     CDS             5075..6202
                     /gene="dxr"
                     /locus_tag="PLS88_03445"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOU06435.1"
                     /translation="MVLGASGSIGRQTLDVIRKSADSEKKLELVGFSIHSNSIAAAKL
                     KREFPQAKGAWTGYTSIAPKEMDWNGIDAISHLLSETEAEIVVNGIAGASGLSASILA
                     LQNGKNLALANKESIVMGYRLLKSIAQENRCQIIPVDSEHAGIFQIVQRFGKSIVSEL
                     IITASGGPFYSLPLELLDTVTADEACKHPIWQMGRKISIDSATMANKGLELIEASRLF
                     DMPQEQIKVLIHPQSYVHAMVRTIEGAIFAQISKPDMRLPIQMALYWPESVQASFGFA
                     DLAGMSLDFYEPTQDRYPMLWIARQAIDEGEAQCIVFNAANEIAVECFDNGKIRFTDI
                     ANLVAKVLDRDWNLPIESFEDIFDIDIKARKIAAHLSENIE"
     gene            6199..7551
                     /locus_tag="PLS88_03450"
     CDS             6199..7551
                     /locus_tag="PLS88_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOU06436.1"
                     /translation="MISIILGLIGLSIVVIFHEFGHFVFARLSGIEVEAFSVGWGPSI
                     VSHKGKKTEWKLCAFPIGGYCKLKGEEGFQAALDQNLQYIPSEKGSFFSASPLKRIFI
                     ASAGPLFSVLFAAFIFILAVSIGTTTVTAPNKIILSSEIGSTLQNEMPNPADIAGLKT
                     GDKIISIDGKEISDYSDLQEIIAINPGKTMSVEVLRNGEAHSLLITPRLDPNTGVGVI
                     GVYAWIDPIVESVQEKSPAAIADLHPGDMIIEADGKVISNTIDLMQVFNNANGSVSLR
                     VKRGDQTLSMVVVAKSLEETGLRFEGILRTEKAKSLPEAISMGVEETFSTLSLEIKSI
                     GLLFRGVNVFKAISGPARLTYLIGTAASEQIKTEGLAGLVPILDFLAFISIGLAIMNL
                     LPLPALDGGRILIYLIELIRRRPINAKSLYRYQFIGAASILLLLLIATMSDIFFFLGK
                     "
     gene            complement(7646..8989)
                     /gene="dnaB"
                     /locus_tag="PLS88_03455"
     CDS             complement(7646..8989)
                     /gene="dnaB"
                     /locus_tag="PLS88_03455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOU06437.1"
                     /translation="MAGPSLKDKLPLYNGDAEQACLGALLLDPESINNVLRYLRPDSF
                     YDSANQEVFEALLAMHEKGQKPDLITLSEELRSRGSLERIGGSAYIASLASFTPSAAN
                     IEYYAKIVQEMATRRRLVHLSAETAAMAHEETKDIDAVLDEIQAKIFEISQNRRTADY
                     HSAKEIVTETMLLIEKLSTDPSAFTGIPSGLTDLDAITNGLQKSEFIVIGARPSVGKT
                     ALALTMAAHASINLKIPVAFFSLEMSESAIMLRLIASEARIPADRIRTGRIKTTDYDS
                     LMEAASRIYEAPLYIVDLPNMKLLELRTMARRLVLERGVKIIFIDYLTLITHENADLP
                     RWEQIAAISRSLKALARELDIPVVALSQLKREAEGKQPTLADLRESGSIEQDADLIIF
                     LHRDREISKTYESQSDQIETDIIVAKQRNGPTGKAPAWFKSSYAKFVNMERRDRT"
     gene            complement(9040..9633)
                     /gene="rplI"
                     /locus_tag="PLS88_03460"
     CDS             complement(9040..9633)
                     /gene="rplI"
                     /locus_tag="PLS88_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOU06438.1"
                     /translation="MKVILNQDIPNLGEIGDIKEVASGYARNYLLPKKMVMVYNEKTE
                     AMFLKRQAEIQAIKEQKRLSSRSLKERIESEPLVISMPAGKNGKLYGAVTSHTIAEEL
                     LKKNIEIDRKRIEVPDRTIKSVGNYKILIRLYEKDEAILHCNIEAQASASIEEKKPSE
                     EVGKKRRSRRQRTESEPASPEEQAAAFEAAVNRGHLS"
     gene            complement(9636..10610)
                     /locus_tag="PLS88_03465"
     CDS             complement(9636..10610)
                     /locus_tag="PLS88_03465"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOU06439.1"
                     /translation="MEASQSIKSASSSKYSWRVFIPFGFLSGFLYASGFLAFAFLIPI
                     QYVFRKENKKEGLLSMLVSLLIIAVISAIELSKMNWIELPMLLQTLILPALLLATIGA
                     MNLLKGDAWVKIIAATVVLSILFAYIFSISLGTPEVQRSMADIIANLLSSSGIQDLDA
                     STIEQSYVIPAVSIIIRLIGAASWLLLSGSWHIGNSLASSRSSKQEGETKKKAFVFNV
                     PSWLLWPSILAWLALLCVLYGNISGIFAIIAWNASLAAACWYAFQGFSLISHFFQSRN
                     MPRITSLLLVLLVILMFFEAKIRIAVAILAPMLGVSEVWLQYRIHKGA"
     gene            complement(10651..10968)
                     /gene="rpsR"
                     /locus_tag="PLS88_03470"
     CDS             complement(10651..10968)
                     /gene="rpsR"
                     /locus_tag="PLS88_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOU06440.1"
                     /translation="MDEVRDIDESRASNAEADQQELHRDQDDRNGVRKGKGFFRKKVC
                     RFCTQNVKIDYKDAELLRRYTTERGKILPRRITGTCSKHQRMLAVNIKRARALGLLPY
                     VIK"
     gene            complement(10981..11436)
                     /gene="ssb"
                     /locus_tag="PLS88_03475"
     CDS             complement(10981..11436)
                     /gene="ssb"
                     /locus_tag="PLS88_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU06441.1"
                     /translation="MSDINVVVLVGRLTRDSELKYTPSGLPICRFSIAVNRSKKQDDQ
                     WVDEPHYFDIEFYGKSAEGLSKYLLKGRQVAVQGELRQDRWEKDGQQRSKVVIVAGTI
                     RPLGAPPQNSGEMGGRRYAADTIPSKTESANNQMPIPPAIDEFTDDIPF"
     gene            complement(11470..11751)
                     /gene="rpsF"
                     /locus_tag="PLS88_03480"
     CDS             complement(11470..11751)
                     /gene="rpsF"
                     /locus_tag="PLS88_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOU06442.1"
                     /translation="MRQYELVTVLNSEEEEFKSGKQAIVDLLSQYKAIDVKEEDMGDR
                     PLSYPIKKKTRAHYVLYRMSFDPASIASLERAIKLNPLVLKHLVVKIEE"
     gene            12029..13360
                     /gene="hisS"
                     /locus_tag="PLS88_03485"
     CDS             12029..13360
                     /gene="hisS"
                     /locus_tag="PLS88_03485"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOU06443.1"
                     /translation="MPIIKPRVLKGFRDFLPDAEITRSSLVKKLEQVFSLFGFVPIDT
                     PVLEYADILLGKGGGETDKQVYRFLDHGDRDIAMRFDLTVPFARFMAEHVEELYLPFR
                     RYHIAKVWRGENTQRGRYREFMQCDFDIVGTDSSSADADILLTAAKAMIVLDVGEFSL
                     RINHRALFNRFLARIGALDKSAEILRTVDKLEKIGFENTLEKLSKLVGLELAADILSF
                     IKKEDSFEATLEKMISFCNKNDVYAQSAKSRLLEIMECVAATGFDNRIVLDPSITRGL
                     DYYTGMVVETSLGALPEIGSVCSGGRYDELASLYTERKMPGVGASIGLDRLIAALETI
                     GKINDSVISTQVLIMNQEKGNAESIAISELHALAAHLRDAGFSCEVFPEEKKTLAQYT
                     YAERKHIPLALFANSAKGPYNLRILAQRKNIECLDFDSLCESIRSSLNVKT"
     gene            13347..14093
                     /gene="trmB"
                     /locus_tag="PLS88_03490"
     CDS             13347..14093
                     /gene="trmB"
                     /locus_tag="PLS88_03490"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOU06444.1"
                     /translation="MLRPEMEEKGQGQKSKKYQSMSQEIGLIDGSVHSFVKRSGRITE
                     GQRRAIESLGPKYIIPYSDKFLVTETLFPQIKPIILEIGFGSGQATWRIAKERPEFNY
                     LGIEVYAPGVGRLIMDLELHVIENVRILQQDAITVLKHMIAPASLAGIHVFYPDPWPK
                     KRHHKRRLMQYPIVCLMAQKLIQGGYLYCVTDIEEYACFAKDSLDACSLLENVYGSFA
                     PHQQWRPETRFENLAKEAGSEPFELFYKKN"
     gene            complement(14098..16782)
                     /locus_tag="PLS88_03495"
     CDS             complement(14098..16782)
                     /locus_tag="PLS88_03495"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA];
                     GO_process: GO:0008380 - RNA splicing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HOU06445.1"
                     /translation="MNPHDLPIYQQKARILEALSKNQVIVVESPTGSGKTTQLPIILY
                     EAGYAADGKIGITQPRRIATLSVSDFIARQLNTTVPGVVGYKMRFEDVTAPNTAIKIM
                     TDGILLQEMKFDPYLSSYSIIMVDEAHERSLNIDFILGLLKKVIDTRNDFRIIVSSAT
                     INAEVFSEYFGECPIVRIDAPMFPVQIRYDAIRPASDIGSKYISSTKESEEIYFRSSD
                     EALYEKILSIIDRVLRGETDAEPGDILIFLPGEKTIKECLQVLMTSRWASQLDCIPLY
                     ARLGKDEQERVFESPPPGKIKVVIATNIAETSVTIDGITVVIDSGLAKINYYNPKTFT
                     ASLIETPISKASCNQRKGRAGRTRPGICYRLYSLKDFESRPLYPTEEIRRTDLSEVLL
                     RMAELGITDFEHFDFITKPPKSAIRGAIEALNMLDALNPDRSLTHIGEMMCAFPLLPK
                     LSRMIVEAILKYPGVLSETLIAAAFLSTTSPYVLPPGEETEARAAHHRFRDPMGDFAS
                     YLKLYEAFSSARDKTKFCEHNYLDERTLREILRIKEQLDLIVSDMGIPIGSGGSIGDY
                     LCAVSRGLIQFVCAQQGRGMFSSLTAEKIHIHPGSVMFRQDADFIVAGEIVRTTRMYA
                     MSVSPLSRSQLYRISPELAEQIIALKQKEPISISRSREDRELKETISDRKHLPKESKL
                     GQISKKDNKVERRVKVIGSGQNETILLGGEVFEIDSKKPKRTVVLDWVQLTKILNKLE
                     PSDKLISKGLRARVEWNGMILFNGEKFSTVVKASKWIDPDRDMNAASPPNMNFSLKEG
                     TSEGLQTLDALVASFDQILRVVRIGNSNKLKFVGLHSDSAGNFWYQPASHFADAIAQS
                     LASMELLVDYAKKYNAPLSLKEKISMYYRRLSDFFNMI"
     gene            16867..18120
                     /gene="xseA"
                     /locus_tag="PLS88_03500"
     CDS             16867..18120
                     /gene="xseA"
                     /locus_tag="PLS88_03500"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOU06446.1"
                     /translation="MSFDYNFDAETHLSSPLTVSQITSLIRKDLEGKYPDVIIEGEVS
                     NCKIAASGHLYFSLKDEDAVLQAVMFRRDLLTLAFVPKDGMRVWARGAISVYAQRGQY
                     QLIARSMRQTGFGDILAMLEQRRRKFAEEGLFDESRKKKIPIFPTRIGVVTSPTGAAI
                     RDIIRVLHRRNPKVSILILPTLVQGQEAAASIAARIQQANRWNLADVLIVGRGGGSIE
                     DLLPFSEEVVIRAIAESHIPIISAVGHETDWALSDYVADLRAPTPSAAAEMACSDLDL
                     IYKEFTHFENILGQSMKHALASARQRLSSSSPRILEDIILRKNIVASQRFDAAIENIR
                     NCFQKRIEDLNHRLVIASSAIEIANPKAVMKRGFSVVKKNGTDIVRSSRTLAQGDFIN
                     IMFYEGEADADVRSIRNASDKRSQG"
     gene            18117..18389
                     /gene="xseB"
                     /locus_tag="PLS88_03505"
     CDS             18117..18389
                     /gene="xseB"
                     /locus_tag="PLS88_03505"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOU06447.1"
                     /translation="MKDFEKRITHLEELAEKMREPDLPLEKSFSLFEEAVVLARELRK
                     ELDELQGKVEILLNSLEIRENDQAEVPNIANFDISTSESQIDSEEK"
     gene            complement(18409..18867)
                     /locus_tag="PLS88_03510"
     CDS             complement(18409..18867)
                     /locus_tag="PLS88_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU06448.1"
                     /translation="MSGQDDTTDMIEYLRSVAGIAIQSDLLFIARRKQDHTEMSHRWE
                     FPGGKVEEGESDESALKREFLEEFGADIDVIRFLGESVFQNKGKTRTLAAWQVSLSSG
                     QIQHLNEHVEVAWLPIEYISKMELADSDRSLIPIVEAAMNSNSGSGHQVG"
     gene            complement(18883..20565)
                     /locus_tag="PLS88_03515"
     CDS             complement(18883..20565)
                     /locus_tag="PLS88_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HOU06449.1"
                     /translation="MHALISNKNSLELDALTRTEFNFSVDHLMEIAAIRLWQTLVSEV
                     FLKKDISNLISANSVSSQQLNIAAVCGKGDNAGDALTMLRHARLGGYSRLTAYIPEPC
                     TMKPDVILNLQRAERCGVKIICYETVDPKSISEQLSTNDVILDALFGTGIDGPAKGKS
                     SDLIRLLRDIRISRNEMGKKNPLIVSLDVPSGLSDAWKPEFPIVCADVTLCIEPMKES
                     LYMPSARPYAGDIFPVGGIFPQWTKSSTQNTWLLEESDMKSFLPPISPWAYKMQRGRV
                     AVFAGSSSGAGAALHCIHGVASAGAGYIALFCDKELFSAYLASIGENAIVRVFSKDSF
                     SPECWDVVIAGPGWGIDLERERILAMLFQTDIPLVLDADGARMFARIASKNQDSSFCR
                     APIILTPHPGEFSEMLPILKSVPLDPLANVSETVALVTAFAKKYGTTLALRASTTHVG
                     LPDGSCYIYDGSTPGLGIAGSGDVLSGMIGGFLARWLAFLKETGENRESTNAAIPSDN
                     PMTKALLGAILVHGSAGKQLWLKKGWFTPIDLANACARISSGVMDTNVYDDR"
     gene            20679..22001
                     /locus_tag="PLS88_03520"
     CDS             20679..22001
                     /locus_tag="PLS88_03520"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU06450.1"
                     /translation="MNDFASLGINANLINALEILGFHEPTEVQKHTVPLLLEKKDLSM
                     QSETGTGKTLAYLLPIYNSILSNIEEANRRWPIAAIICPTQELAVQVANHSTRLAMAL
                     EAKDKGAISESGSSQLQESSQGNARAPKTISNHSNSPQIRSLVLLGGTQFSRQKQALR
                     MHPHIIVGTPGRLADLLRLRYFDFSHLEFLILDEADRLFSREYIEPVEYLLSNAPRLC
                     TRVMASATIPERIRNSAKPWIPKPIVIELESEGVLSQAIEHWAFYSDHRKKIDLLKKI
                     IVAIHPKRCLIFASEAYRVQRAADRLCSSGLVCASLVSRMEKQSKHSIIEQFKTGSIP
                     LLITTDLAARGLDIPDITHVISLDLPEDGGVYVHRAGRTARAGKKGISILVADKLELE
                     RASRTATRFGFVFRTKWLEYGTVVEPSVEAFFERIEDMETQKRSKSHL"
     gene            21998..24544
                     /locus_tag="PLS88_03525"
     CDS             21998..24544
                     /locus_tag="PLS88_03525"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU06451.1"
                     /translation="MSASAIHEPQIITEAVAADMANPLFEDAQYRILIVRLSPFRDVE
                     SSYTHLVLFDETRRALPAAYIDFAFMPNLPHKKALSMENRPWFLGRASNRSALEFNML
                     LISCAFSLELLNIPWLLTQSGIPFSRQERMENDSLPFLLLGGSSAVCSGSLVKIADNR
                     VIDSLVDAMFFGEGEGRISEIVRIAAEDSRSGLSKSSIIAHIASEIEGFWPCDSSFAC
                     KRALSTQRPAVLTSPIMLNSENADSIKLAITAGCIGHCTFCLEGWDRRPFIEKPVDHL
                     SKSAIMLKRASGASDVELFSYNFNMHKNIIQLIQIFGKYFMHVSMMSQRLDILYKKPE
                     ILAAELAAGKRSFTLGIEGISERIRNYYQKGISEEQIWTSITRILENRAREIKLFFII
                     SGFEEGSDLEEFAHFCDRLAHHKIETHSVTRVIVSAGYLVRLPFTPLQFAPLQGNRAL
                     MESIASALCKSCKQANLEFRLASSFEDYWMDQLLSLGGSIAHDWLQTCPKNGFFYDLH
                     VPSRALESLCAYFEKQPIFNQLLEEKPKTYRPDFYFIESDRHWQMLAALYDQSLNYLH
                     NRDSRNSYIENHSGSSISIEAKKTIDIIKAQQKAKAHFPSILIKISENNALAFSTPAY
                     ERTWLLRTLSALVPNSELGFFYCNLQLPNLDWESSMPISSPSLVYRSRLGISGVKYFA
                     IYGPDITNMKKVISLTATALKNVRGIESISNSSAYSGSTLFLEDIELIQELPTAKVCR
                     LSACIPADKSRLINEALEEWLSEMGLHFTLKKDEDSIIYYTSSINKTNKALKYIKYKT
                     ISTNICLSLCIGKKANLRLLADILYKKCTIEKTIFRIEGWDLNALDLDDH"
     gene            complement(24653..25021)
                     /gene="queD"
                     /locus_tag="PLS88_03530"
     CDS             complement(24653..25021)
                     /gene="queD"
                     /locus_tag="PLS88_03530"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOU06452.1"
                     /translation="MYHIRVEAEFSAAHRLVHYNGKCERFHGHNYKVRVWASGKDLGE
                     GGMLIDFGILKSALRELLDKEFDHQDLNQIAELENDPSAERIAALIYTRLHNGFPCLP
                     ISAVDIFETSSTMARFVPDE"
     gene            25193..26887
                     /locus_tag="PLS88_03535"
     CDS             25193..26887
                     /locus_tag="PLS88_03535"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOU06453.1"
                     /translation="MQKQLSDLIKGLDISSIEGDVHHLISDLSYDSRECKAGSLFFAL
                     PGLHTDGKEYIIQAIQNGSVAVVYEGMLEKRLREFLLKEPLTKAINPALIQVIDCRWT
                     MSAISARFFENPSDSLCVIGVTGTEGKSTTVSLIYQLLNATGFKAGFFSTVMSDIGEG
                     EKPNPQHQTTPEATTVQQMLAEMRDHGLGFAVVEASSHGLSPRTARLAHVHFDIGLVT
                     NVTHEHLEFHGSWEQYRSDKANLFRRLGEAGVKNLDRVDLRAKEHKKIRILSPVGIIC
                     ADDPNASYFAHESAANCLTYSSKGAPAYLSAHNIIGDPNGASFDIEGPLEMAIDNGLQ
                     PKHITGSQALKAAPLLTLQARINLPGNFNVQNALGAILAASTATGLHWRNFVPFLPYL
                     KPIKGRMQRILEGQTFEVIIDYAHTPSSFKEILPTLRRERKGRIICVFGSAGERDREK
                     RPRQGRIAADYCDILILTDEDPRGEEPMAILEEIASGCPELSKEDRLYLIPDRPAAIR
                     KAFSLAKPGDLVLLLGKGHENSIIYSDRTIPYDEETQARSILADMGLGLRAKGNKT"
     gene            26884..27999
                     /locus_tag="PLS88_03540"
     CDS             26884..27999
                     /locus_tag="PLS88_03540"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOU06454.1"
                     /translation="MKNIAVLYGGRSGEHEVSLISASSILAYLDKEKYSIVPIGITKR
                     GEWFLQYPPEWAYLPPEKGQMRSLPPVEKTERVLAVPGEGLCLETKKGVQKLDIDVVF
                     PVLHGTFGEDGTIQGLLETVDLPYVGADVLGSAVSMDKEVSKRLWLSAGLPVVDYISV
                     RKEDVSDENLKILARRVETRFGWPCFVKPSCSGSSVGTSKVNNSEELKNAILFALRWS
                     EHALIEAFINAREIECAVLGNERPVVFAPGEIIPSHEFYDYEAKYKDPQGATLLIPAS
                     LSEETKNRIMSLALSAYKYASIKGMARVDFFVEKITGEVFINEINTIPGFTAISMYPR
                     MCINGGVSYIELLDKLIELALEQYALKRELEYDFSKS"
BASE COUNT         8152 a         6021 c         5986 g         7897 t
ORIGIN      
        1 ttttctctga gcgatgcgcg atttgaacgc gcgaccttcg gctccggagg ccgacgctct
       61 atccagctga gctaatcgct cttgtgagat gcagatttag cttgaactca tatttttgca
      121 tattaagact tagatcgata tgcgtcaagc caacgcttat tttgaaaaag cacgtttaga
      181 aatcgattca agacttggca aagtattcat ctctcattag atttccttgt atcatgtatg
      241 gcgaacctat cttgcttgca agtgaatcct cgcgaagggg ttcaattctt gaatcgatca
      301 gaataccatt tattagggtt atgccttcaa tcgacgagac agcgtttgat gacctagatc
      361 ctggagaacg tgtcatagct cttgcaacaa caaaagccaa acgtggcaat tctatttggt
      421 ttgagaaaac ccaaagagaa agagtagtta agtcctctat cgagaaatat ttagaaaagg
      481 gatcttttga tcgagaatgt cttagagaac ctcgattcgt actcggagct gacacgctgg
      541 tagcttttaa gatttccgag ggttggataa cgcttggcaa accagctaac aaactcgaag
      601 ctttcgaaat gctttcgatg gaagcaggta aaagacaaac tgtctttagt ggactttgcc
      661 tgctcgatat ggcaagcaat cgatactacc ctgctctttc tatctccgaa gtcaagtttg
      721 cacccatgac acctgaagaa atctcgtggt acctttgctt cgaggaatgg agcggagccg
      781 caggagcata ccgaatccaa gaaattggtt catgcttcat agaagaaata agaggttcat
      841 cctcaggagt aatgggcttg ccaattcatg aattatatgg cattctccgt gagtcggggt
      901 atttggatcc ctagcaagga gaaacacata ttatcgacaa tccagccttt ttaatgctgg
      961 gcattttccg cctgtctttc tagtagagag caggacgaga cataaggaag ggaaccggaa
     1021 tctattccgg tagtgggagg tctacgtggc agtagtgacc atgaaaaatc tgcttgaatc
     1081 aggcgtgcac tttgggcacc aggtcaagcg ctgggatccg cgtatgaaaa aatacatatt
     1141 cgcggagcgc aatggtattc acatcataga tctgcaaaaa accatccagg ctataaaaga
     1201 agcctatgac gcagtgcaga aggtggctag ttctgggaaa tccgtgctat ttgtcggaac
     1261 aaagaaacaa gcccaggcag ctatacaaaa agaagccgag cgctgcgagc aattctatgt
     1321 aaataatcgc tggttaggtg gcatgttaac aaacttttcc accataagaa aatctgttca
     1381 aaggctcaag aagattgagc gtatggaagt agacggaacc tttaacagtc ttaccaaaaa
     1441 ggaaatcgca agtcttcaaa aggaaaaggc caagcttgag aagaacctcg gcggaataaa
     1501 ggagatgacg agcctaccag gtatcctttt tgttgtggat acgcgcaaag aggcaatcgc
     1561 ggttgcagag gctcagcgcc ttggcattcc tattgtggca atagttgata ccaactgcaa
     1621 tccagaaggc attacttatc ctattccagg caatgatgat gctattcgat ctataagcct
     1681 tttcactcaa atcatagcaa atgcggtaat cgaagcaggt gcttccgaag gcttaaagat
     1741 aatcgaaacc ctgcctcctg aagaggagca agaactggtc attcctcaaa aactagagga
     1801 agatgaggtt gagatcgatt tagataatta tgccgaatcc tccagtcaaa gcaacaaaga
     1861 aacagatgag cagcaggaag tcgaacaacc cataattgat gaagatcgcc tttatgatga
     1921 aaattgagaa aattgaggag aatagatcgt ggaaatcaaa gcaaatgatg taaaagctct
     1981 gcgcgaaaaa actggtgcag gcatgatgga ttgtaaaaaa gcgctcatag aagctaatgg
     2041 tgatttttct gcagctgaaa agttattgcg cgaatgggga attgctggaa tcgaaaaacg
     2101 agctggccgt gcaacaaatg aaggtcgaat tttcattgct caaaatgcta aagaattgga
     2161 gctaatcgag ttagcttgcg aaactgattt tgtcgctaga aataaggact ttatagcagc
     2221 gggcacaaaa attgcagaaa gagccctatc acagaaagca tccggcacaa atgagagctt
     2281 agaaacaatt gtaaaggaaa tagcttcgct cataaaggaa aatattgcgc tgaggcgcgt
     2341 tgttcatttt tatgccgaag aaaacgatat tttgcacgcc tatttacatg gcgaaggcag
     2401 aattggtgtt gtcgtaaaat ttagagcgaa caaccctaag gctttcgaga atgagaagat
     2461 cactgccttt atacatgata tagctctaca tgtagcagcc ttcaacccta tgttcctcga
     2521 tgaatcaaaa gtttccacag actggattgc agaacaaaaa gaaattttcc aaaaacagct
     2581 cgagctcgac gaaaagttaa agagcaaacc cgaaaaagtc cttgaaggca tcttatctgg
     2641 aaagctcaag aagcttcttg ccgaagtctg tcttctaaac cagggattcg tgcgagacga
     2701 gaagtcatct gtcgctgccg ttctagctca ggtctccaag gaaacaggct atcaactcga
     2761 aatcatagac tattattttg caaaagtcgg acaagattga atatgcccag ctaatgcaag
     2821 ggcataaaag ggcgatctac tgagattgcc cttctgtacc ttcaaaatag tacatcaagg
     2881 aagaagatat ggaatcgaac aaagctgccc atgaagaaaa gatgaaaaag acaatcgctg
     2941 cgtttcaaga tgagctcaat agcatacgca caggcagagc ttcctctgtt cttctcgaca
     3001 aggttagagt cgactactat ggtcaaaaaa tgcctctttc ccaggttgcg acaatttcgg
     3061 ttcctgaagc tcgtctcata gttattcagc catgggaccg ctccttattc agcgaaatcg
     3121 aaaaggcaat cctcaagtcg gatttaggtc ttaatccttc aaatgacggg aaagtgctta
     3181 ggatagcgat acctcctcta accgagcaga gaagaaaaga gctcgttaag acagcccgca
     3241 gtatcgccga acaagcgcgt gtagcaatca gaaatataag acgcgatgct ctagaagagc
     3301 ttaaaaagca acagaacgca ggaggtgttg ccgaagatgc cctcaagaaa gaagaagcgg
     3361 agctccaaaa aatgactgat gattacattg cccaaatcgc aagaattctc gaaatgaaag
     3421 aaagagaaat aatggaagtc taaggattct ttgagatgaa agagaactgc atgccttctc
     3481 atgttgcgat cattatggat ggcaatggtc gatgggcgac acaaagaggc ctttctagga
     3541 cagaaggaca caaggaagga ttagaaacag cgaagcgtat agtacttgcc tcacacaaag
     3601 aagggatcaa gtttctctcc ttatttgttt tttccacaga gaattggaag cgtactgaac
     3661 aggaagtcgg atttcttatg tctcttattc gagttcatat cacaagagaa ttggatttct
     3721 atcgagaaaa tgagatacgc attgtccatt ctggtgaaag aagtggacta ccacatgatg
     3781 tactttctga aatcgataag gcgattgaga atacggctca ttaccgttca ttgacagtca
     3841 acctggcgat caactatgga ggtagagacg aaatagtccg agcaatgcag cgaattctag
     3901 caagtcaggc aaatggcgac aaaaaaccga tagatgaatt gacagtcatg caatacctcg
     3961 atcatccgga tatacctgat cccgatctca ttatcagaac aggtggcgag atgaggttat
     4021 ccaattttct attatggcaa tctgcttata gcgaactata cttttcgcca aaatattggc
     4081 ctgattttac tccaggggat cttcatgcgg ctcttgtcga ctatatgcac cgtgaaaggc
     4141 gttttggaga tgcgcggtga atgagaaaaa tacaagggca agacttcttc ttttttttat
     4201 aggtattcct ttactcattc tttcactttt ttttcttcaa tttgctcatt atataataat
     4261 ttccggaatt attttcgttg tacaattcct ttcatccaag gaattgcgaa atatgctaac
     4321 tagagctgct atgccagatt caggagattc attaaccttc attggcttaa ttccatcagt
     4381 attgacatat atatcatgtt ttctttcagg atcgccccta gagactgcat ttgtcttgtt
     4441 tattgcttgg tcattctgcc taatactgat attggttccg cttgcattcc aaaaaaagaa
     4501 ttcatttcca gagcttatca agcaagcggc atcaatcgtg cttgttcact tttatacagc
     4561 acttcttcca ggatttctaa tattcattgt ttcagggttt cctaattcac agtatacaat
     4621 aatcaccttt gctatgctta catttggaaa cgacagcctc gcatggcttt ttggtaaata
     4681 ccttgctaag aaaagaaatc ttctggatgt gtcaccaaac aagagtgttg cgggtttcat
     4741 aggaggcacg attggatcga taatcgctgc ttttatagga ttaggaccaa tggctggtac
     4801 ttggaagccc tctggagcct catatacact gctaagccta tctcttgggt tgggaatggc
     4861 attctttgtt atcgtgggcg acctctttga aagcgctctc aagagatcgg caagaatgaa
     4921 ggattcaggc tctatcgtac caggcagagg aggaattctg gactcatttg atagcctata
     4981 ctttagtgct cctttttttg ttgctttttc tttttcattt catttgttta atctataaaa
     5041 gcaagagtat tggaggtaaa agctgaagcg tgtcatcgta ctgggggctt ctggctctat
     5101 tggcagacag actcttgatg tgattagaaa gagcgcagat tctgagaaaa agctcgagct
     5161 tgttggtttt tctattcata gcaacagcat cgccgcagca aagctcaaga gagaatttcc
     5221 tcaagctaaa ggagcttgga caggctatac atctatcgct cctaaagaaa tggactggaa
     5281 tggcatcgat gctatatcgc atcttctatc ggaaaccgaa gcagaaatag tcgtaaatgg
     5341 aatcgcagga gcatctgggt tatcagcatc aattctagca ttgcagaatg gaaaaaactt
     5401 agcacttgca aataaggaat cgattgtcat gggctataga cttctaaaaa gcattgccca
     5461 agaaaatcga tgccagatca tacctgtaga ttctgagcat gcaggaattt tccaaattgt
     5521 tcagagattt ggcaaaagca ttgtctccga actgataatt actgcatcgg gtggaccatt
     5581 ctattctctt cctcttgaat tgcttgatac tgttacggca gacgaagctt gcaagcatcc
     5641 tatatggcaa atgggaagaa aaattagtat tgactctgca acaatggcca ataaagggct
     5701 ggagttgata gaggcttcta ggctttttga catgcctcaa gagcaaatca aggtgcttat
     5761 tcatcctcaa agctatgtcc acgcaatggt tcgtacaatc gaaggagcta ttttcgccca
     5821 aatctcaaaa cctgatatgc gcttaccaat acaaatggcg ctatattggc cggagagtgt
     5881 gcaggcttcc tttgggtttg cagacttggc aggtatgagt ctcgatttct atgaacctac
     5941 gcaagatcga tatcccatgc tgtggattgc acgccaagct atcgacgagg gagaagctca
     6001 gtgcattgtg ttcaatgcag ccaatgaaat cgcagtagaa tgcttcgata atggaaaaat
     6061 tagattcacc gacatagcca acctagtagc aaaggtactc gatcgagact ggaatttgcc
     6121 tatcgaatca ttcgaggata tattcgatat agatatcaag gctcgcaaaa ttgccgccca
     6181 tctctcggag aatattgaat gattagcatt atcttaggcc ttattggtct ttcaatagtt
     6241 gttatatttc atgaatttgg ccactttgtc tttgctcgct tgtctggcat agaagtagag
     6301 gccttttctg tgggttgggg accttcaata gtttctcaca agggcaaaaa gactgaatgg
     6361 aagctttgtg ccttccctat tggaggatat tgcaagctaa agggagaaga gggatttcaa
     6421 gcagcgctag atcaaaatct tcaatacatc ccttcagaaa aaggtagttt tttttcggcc
     6481 tctccattga agcgaatttt cattgcatct gctgggcccc tcttcagtgt actttttgct
     6541 gcttttattt ttattttagc cgtatctata ggaactacaa ctgtaacagc accgaataag
     6601 atcattcttt cgagcgaaat aggatccact cttcaaaacg aaatgcccaa tcctgcagat
     6661 atcgcaggcc ttaagacggg cgataagatt atctcaattg atggtaagga aataagtgat
     6721 tacagcgatc tccaggagat cattgctatt aatcctggaa agaccatgtc tgttgaggta
     6781 ttaagaaacg gtgaggctca ttctctcctt ataactcccc ggctcgatcc aaatactggt
     6841 gttggcgtaa ttggcgtata tgcttggata gatcctatcg tagaatcagt gcaagaaaag
     6901 agccctgcag caattgcaga tcttcatcca ggtgacatga ttatcgaagc agatggaaaa
     6961 gtcatctcaa atacaataga tcttatgcag gtattcaaca atgcaaatgg ctctgttagt
     7021 ctcagggtaa agcgcggaga tcagactctc tcaatggtcg tcgtggcaaa atctcttgaa
     7081 gagacgggcc ttagattcga aggtatcttg agaactgaaa aagctaaatc gctcccagag
     7141 gctatttcga tgggagtaga agagactttc tcaactcttt cattagaaat aaagagcata
     7201 ggacttcttt ttcgcggtgt aaatgtcttc aaagccatct ccggaccagc cagattgaca
     7261 tacctaattg gaaccgccgc atccgaacaa ataaagactg aaggtctagc agggcttgtg
     7321 ccaattcttg atttcctggc ttttatatcg attgggcttg ccatcatgaa ccttcttccg
     7381 cttcctgcat tagatggtgg tcgtatcctt atttatttga tagagcttat tagaagacgc
     7441 ccaattaatg ccaagtcgct ttatcgatat cagtttattg gggcggcttc tattcttttg
     7501 ctattactta tagccacaat gagcgatatt ttcttctttt tgggtaaatg aggatttagc
     7561 ttatggaaat aatccaaagg taggctcttt aagatataat atctggaaaa ccgcttgctt
     7621 tctgctatgg aaaatcgaca cttccttatg tgcgatctct gcgctccatg ttcacaaatt
     7681 ttgcatagct gctcttgaac caggccgggg ccttacctgt tggtccattg cgctgtttgg
     7741 caacaataat atctgtttcg atttgatcgg attgtgactc ataagtcttg gaaatttctc
     7801 gatctctatg aagaaaaatt ataagatctg catcttgttc aatcgagccc gattcacgca
     7861 aatcagcaag agtcggctgt tttccctctg cttcgcgttt tagctgcgaa agagccacga
     7921 cagggatatc aagttctcta gcgagagcct ttagtgaccg cgagatggct gctatctgtt
     7981 cccaacgagg caaatcagcg ttttcatgtg ttattagggt aagatagtcg atgaatatta
     8041 ttttgacccc tcgttcgagg actagccttc gcgccattgt tctgagctca aggagtttca
     8101 tatttggcag gtctacaatg tagagaggag cttcatagat tctcgaggct gcctccatta
     8161 ggctgtcata gtcggtggtc tttattcggc ctgttcttat gcggtcggca ggaatccttg
     8221 cctcagaagc aataagcctc agcatgattg ctgattccga catctccaaa gaaaaaaatg
     8281 caactggaat cttgagatta atcgaggcat gagctgccat tgtaagagcc aaggcggtct
     8341 ttcctaccga aggcctagcc cctataacaa tgaactcaga tttttgaaga ccattcgtta
     8401 tagcatcgag atcagtgagc ccagaaggta taccggtaaa agcgctggga tcagtgctga
     8461 gcttctctat aagcagcatt gtctccgtta caatctcttt ggcggagtga tagtcggcag
     8521 tcctgcgatt ctgcgatatc tcgaatatct tagcttgtat ttcatctaga accgcatcta
     8581 tatccttggt ttcctcgtga gccatggctg cagtttcggc agaaagatgt actaggcgcc
     8641 gccttgtagc catttcttgc acaattttcg catagtattc gatattcgct gctgaaggag
     8701 taaatgaggc tagactggca atataggcac tccccccaat cctttcaagg ctaccgcggc
     8761 ttcgcaactc ttctgatagt gttatgagat ctggtttctg tcccttttca tgcatggcaa
     8821 gaagcgcttc aaatacttcc tggttggctg aatcataaaa gctatcagga cgcagatatc
     8881 ttaaaacatt gtttatcgac tcggggtcta aaagaagagc ccccaagcat gcctgttcag
     8941 catctccatt atatagcggc aacttgtctt taagcgatgg acctgccatc taaaaatcct
     9001 ctagatatac cctaccctct tatagggtta aatactagat cagctcaaat gaccacgatt
     9061 tacggctgct tcgaaggccg ctgcctgctc ttcagggctg gctggttcag attctgtacg
     9121 ttgcctgcgg cttctacgct ttttccctac ttcttcggaa ggctttttct cctctatact
     9181 cgctgatgct tgagcttcta tattgcagtg aagtatggct tcgtcttttt catacaacct
     9241 gatcaggatc ttatagttgc cgacagattt aatcgtgcga tcgggtactt cgattctctt
     9301 tctatcgatt tctatattct tttttaaaag ctcttctgca attgtgtggc tagtaactgc
     9361 accatacagc tttccatttt tgcctgcagg catcgagatg acaagaggtt ctgactctat
     9421 tctctctttt agagagcgag aagaaagcct tttctgctcc tttatagcct gaatctccgc
     9481 ttgtcgcttc aaaaacattg cttcggtctt ttcattgtaa accatgacca ttttttttgg
     9541 caaaagataa ttgcgcgcat atcctgacgc aacttctttg atgtctccta tttcacccaa
     9601 attgggtatg tcctgattta atataacttt catattcagg ctcctttatg tattcgatat
     9661 tgcaaccaca cttccgatac accaagcatc ggcgccagaa ttgcaacggc aatcctgatc
     9721 ttagcttcga aaaacatgag tatgacgagc aaaaccaata ataagctagt tatgcgcggc
     9781 atatttctag attgaaaaaa atgggaaatt aagctaaatc cctgaaatgc ataccaacaa
     9841 gcagcggcta gagaagcatt ccaagcaatt atggcaaaga taccacttat attcccataa
     9901 agcacacaga gcagagcaag ccaagctaaa atgcttggcc acagaagcca tgaaggcaca
     9961 ttgaatacaa atgccttctt ctttgtctct ccttcttgct tggatgatct actagatgca
    10021 agactattgc ctatatgcca actacctgaa agcaacaacc acgatgctgc accaatcaat
    10081 ctaataatga tcgatactgc aggaattaca tagctctgtt ctatagttga tgcatctaaa
    10141 tcttgaatgc ctgatgagct cagaagattg gctattatat ctgccatcga tctctgcact
    10201 tctggcgtgc ctagactaat actaaaaata taagcaaaga gtatagagag aactactgtg
    10261 gcagctatga tttttaccca ggcatcaccc ttgagtagat tcatggctcc tattgttgct
    10321 agaagcaatg ctggcagtat tagagtctga agcaacatgg gtagctctat ccaattcatc
    10381 ttagaaagtt caattgcact tattactgct attatcaaga gagatactag catggagagc
    10441 aaaccctctt ttttgttctc ctttcgaaat acatattgaa tgggaattag aaatgcgaat
    10501 gcaagaaaac cgcttgcata taagaatcca gacaaaaatc caaatggaat aaagacacgc
    10561 catgaatact tgctagacga tgctgacttt atgctctgag atgcttccat caatgtcgct
    10621 cactctttgg atcagattcc aaacgctagg ctattttatg acatagggaa gaagcccaag
    10681 ggctctagcc ctcttgatat tgactgctag catacgctga tgctttgagc aggtaccggt
    10741 aatgcgccta ggaagaatct ttccgcgttc agtcgtataa cgccgaagaa gctcggcgtc
    10801 tttgtaatcg atctttacat tctgagtgca aaagcgacag acttttttgc ggaaaaagcc
    10861 cttcccttta cgaacgccat tcctgtcatc ctgatctctg tgtaattctt gttgatccgc
    10921 ttctgcattc gaagctcttg attcatcgat atctcttact tcatccatat taatctcctg
    10981 tcaaaatgga atatcatcag taaactcgtc tattgccgga ggaataggca tctgattatt
    11041 ggcagattct gtctttgatg gaatagtatc tgcggcatat cttctaccac ccatttcacc
    11101 actattttga ggaggagctc ccaatggtcg aatagttcca gcgacaataa cgacttttga
    11161 gcgctgctgg ccatcttttt cccacctgtc ttggcgaagt tcaccttgaa cagcaacctg
    11221 tcggcctttg agaagatatt tacttagccc ttctgcgctt ttcccataaa actcgatatc
    11281 aaaataatgc ggctcatcga cccattgatc atcctgcttt ttagagcggt tcactgcaat
    11341 agaaaaacgg catattggaa gcccagatgg cgtatatttc aattcgctat cgcgggtaag
    11401 acgcccaact aacactacta cgttgatatc gctcacaata tactccttta gtccttgtgg
    11461 gacatggcct tattcttcaa tctttacgac caagtgtttc aataccaaag ggtttagctt
    11521 tattgcgcgc tcaagcgaag caatagaagc agggtcaaaa ctcattcgat aaagcacata
    11581 atgagcgcgt gtttttttct tgattggata tgagagcggc ctatctccca tatcttcttc
    11641 tttgacatca atcgccttat actgcgaaag cagatcaacg attgcctgtt tgccggactt
    11701 gaattcttct tcttctgagt tcagcacagt gacaagttca tactgtctca ttaaatcctc
    11761 cctgtggaca tatgcactca tacgagtgct gatgatccgg ttgaaagaac cggataaaga
    11821 gggctttatt tatatatcac aaaagaatgc gaaggacaag cacagaaaat gctgcgtagc
    11881 tacctttgca caaaattgtc gtgcaccccc atttgcccac atctcttttg cgtacaatta
    11941 gacctcttgc atccattgcg aattattttc ataattgctt aacttttcat tgcgatatcg
    12001 ttcaattcta tatcgaggga gctggttaat gcctataatt aagccgagag tgttaaaagg
    12061 ttttcgcgat tttcttcctg atgcggagat aactcgctcc tctctcgtaa aaaaactgga
    12121 gcaagtcttt tcattattcg gttttgtacc tatagatact cctgtcctcg aatatgccga
    12181 tatcttgctt ggaaaaggag gcggggaaac agacaagcaa gtataccgct ttcttgatca
    12241 tggtgaccga gatatagcca tgcgattcga tttgacagtg ccttttgcac gatttatggc
    12301 agaacatgtc gaagaactgt acttaccctt taggagatac cacatcgcaa aggtttggcg
    12361 cggagagaat actcagcgtg gaagatatag agaattcatg caatgcgatt tcgatatcgt
    12421 aggtacagat tcaagttctg cagatgctga tattctgctt acggcggcaa aagcgatgat
    12481 tgtactcgat gttggagaat tctcgctccg aatcaatcat cgcgcgcttt tcaatagatt
    12541 tctggcgcgt attggcgctc tggataagtc tgctgaaatt cttaggactg tagacaaact
    12601 tgaaaagata ggttttgaga atactcttga aaagctttcc aagctggttg gcttggaatt
    12661 ggcagccgat attctctctt ttattaaaaa agaagacagt tttgaggcaa ctttagaaaa
    12721 aatgataagt ttttgcaata aaaatgatgt ttatgcgcaa tcagcaaaaa gtcgcctgct
    12781 ggaaattatg gaatgtgtgg ctgcaacagg ctttgataat cggatagtgc tcgatccgag
    12841 catcacccgc gggctggatt attacactgg aatggttgta gagacatcgc ttggagctct
    12901 gccagaaatt ggctctgtgt gctctggagg aagatacgat gaactcgcaa gcctatatac
    12961 cgaaagaaaa atgcctggag ttggagcttc aattggtttg gatagactaa ttgcagcttt
    13021 agagacaatc ggcaaaataa acgactcagt aatatcgacc caagtgctca ttatgaatca
    13081 agaaaaagga aatgcagaaa gtatcgcgat atcagagctt catgcgcttg cagcacatct
    13141 tagagatgct ggcttttcct gcgaggtatt ccctgaggag aaaaaaactc tagctcagta
    13201 tacctatgct gagcgaaaac atattccgct cgctttattt gctaattcgg ccaaaggccc
    13261 gtataatcta aggattctag ctcagcggaa gaacatagag tgtttggatt tcgatagcct
    13321 ctgtgaatcc atcaggagct ccttgaatgt taagacctga aatggaagaa aagggccaag
    13381 gccaaaaatc taagaaatat caatcgatga gccaagaaat tggccttatt gatggaagcg
    13441 ttcattcttt tgtgaaaaga tctggaagaa tcaccgaagg ccaacgcaga gccatagaat
    13501 cattaggacc gaaatatatc attccctatt cggacaaatt tctggttaca gaaacattat
    13561 tccctcagat taaaccaata atcttagaga ttggctttgg atctggacaa gcaacatggc
    13621 ggattgctaa agaaagacct gaattcaact atcttggaat tgaagtttat gcgcctggcg
    13681 taggaaggct tataatggac ctcgaacttc atgtgataga gaatgtacgc atattgcaac
    13741 aagacgctat cactgtactg aagcacatga tagcgcctgc aagcctggct ggcatccatg
    13801 ttttttatcc tgatccctgg ccaaaaaaac gacaccataa acgcagactc atgcagtatc
    13861 caatcgtttg ccttatggct caaaaactta tacaaggtgg ttatctttat tgcgtaactg
    13921 acatagaaga gtatgcttgt tttgctaaag attcactaga tgcttgctcg ctactcgaaa
    13981 atgtatacgg gagcttcgca cctcatcagc aatggcgccc ggaaaccaga tttgaaaacc
    14041 tcgcgaaaga ggctggtagc gagcctttcg agctgttcta caagaagaac tagtgattta
    14101 gatcatgttg aaaaagtcgc taagtcgcct atagtacatg ctaatcttct ctttgagtga
    14161 caaaggcgca ttgtactttt ttgcataatc cacaagaagt tccatgcttg ctagactctg
    14221 ggctatagca tcggcaaaat gagatgctgg ttgataccaa aagttcccag cgctatcaga
    14281 atgcaatcca acaaatttga gcttattaga atttcctatc ctgacaactc gtaggatctg
    14341 gtcaaaagaa gcaacaagcg catctagggt ctgcaatcct tcggaagtgc cttcttttag
    14401 agaaaaattc atatttggag gagacgctgc attcatatct cgatcggggt cgatccattt
    14461 cgaagctttt acaactgtag agaatttttc tccattgaat aatatcatgc cattccattc
    14521 tacacgcgcc ctgagtccct tgcttatcaa cttatcagaa ggctcgagct tgttaagaat
    14581 cttggtgagc tgaacccagt cgagcactac agtccttttt ggcttcttgc tatcgatttc
    14641 aaaaacctct ccgcccagca agatagtctc attctggcct gagccgatta cctttactcg
    14701 tcgctcaacc ttgttgtctt ttttggagat ttgccctagt ttgctctcct ttggcaaatg
    14761 ttttctatca gagatggttt cttttagctc tcggtcttct ctactacgtg agattgatat
    14821 aggttccttc tgctttaatg caataatctg ttctgctagt tctggagata ttctatatag
    14881 ttgtgatcta gaaagaggag aaacggacat tgcatacatt ctggtagtac ggacaatctc
    14941 gcctgcaacg atgaaatctg catcttgtcg aaacattacc gagcccggat gaatatggat
    15001 tttttcagcg gtgagagaac taaacatacc tctcccttgc tgtgcacata cgaattgaat
    15061 aagccctcta ctgacagcgc ataaataatc gccaatagaa ccgcctgatc caataggaat
    15121 acccatatcc gatacgatta gatcgagttg ctccttgatt ctaaggattt ctcgaagcgt
    15181 gcgctcatct aaatagttgt gctcacagaa ttttgtttta tccctggctg aggaaaaagc
    15241 ctcgtagagc tttagatatg atgcaaaatc acccatagga tctcgaaagc gatggtgagc
    15301 ggcgcgtgcc tcagtctctt cacctggagg aagaacatat gggctcgtgg ttgatagaaa
    15361 agccgcagct ataagcgtct cactcagaac acctggatac ttgaggattg cctccacaat
    15421 cattcgcgac agttttggta aaagcgggaa ggcacacatc atttcgccta tatgtgtaag
    15481 agatctatct ggatttagtg catctagcat gttgagcgct tctatggctc ctcgaattgc
    15541 gctctttggc ggctttgtta tgaagtcgaa gtgttcgaaa tctgttatcc ccagttcagc
    15601 cattcgtagc agaacctctg atagatctgt ccttctaatc tcttcggtgg gatagagagg
    15661 acgcgattca aaatctttaa gggaatacaa acggtagcag ataccagggc gtgtacgtcc
    15721 tgccctgccc ttgcgctgat tgcaagaagc cttggaaata ggagtctcga ttaggctagc
    15781 tgtaaaagtt ttgggattgt aataattgat tttagctagt ccagagtcta taacaacagt
    15841 tattccatcg atagtgacgc ttgtttctgc aatgttggta gctattacca ccttgatctt
    15901 tcccggcgga gggctttcaa aaaccctttc ctgttcatcc ttgccaaggc gcgcatatag
    15961 cggaatacaa tcgagctgag aggcccaacg gcttgtcatc aaaacttgta ggcactcctt
    16021 aatcgtcttc tccccgggaa gaaaaatcag aatatctccg ggttcagcgt ctgtttctcc
    16081 tctgagtact ctgtctataa tagaaagaat cttttcataa agagcctcat ccgaagatcg
    16141 aaaatatatt tcttcgcttt ctttggtgct cgaaatatac tttgatccta tatctgaagc
    16201 tggccgaatt gcatcatagc ggatttggac tggaaacatg ggagcatcga tccttacaat
    16261 ggggcattct ccaaaatatt cagaaaaaac ttcagcattt attgtcgctg atgatacaat
    16321 tatcctaaag tcgtttctcg tatcaatgac ttttttaaga agcccaagta taaaatcgat
    16381 gttcagactt cgttcgtgtg cctcgtctac cattattatg ctgtaagaag aaagatatgg
    16441 atcaaatttc atttcttgca agagaatgcc atctgtcatt atttttatgg ctgtattagg
    16501 ggctgtcaca tcttcaaaac gcatcttgta accaacgact cccggtacag tggtgttgag
    16561 ctgacgagca ataaaatcgc ttacagacaa agtcgcaatt ctccttggct gtgtaattcc
    16621 tatttttcca tctgcagcat atccggcttc atagagtata attggaagct gtgtagtctt
    16681 accggaacct gtagggctct ccacgacaat aacctgattc ttcgataaag cttcaaggat
    16741 cctagctttc tgctgatata taggaagatc atggggattc atcataactc tcagtttaat
    16801 gctatgataa aaattcagcc agtatgcgac attcacctaa ggtaaacatt ttggtaaaat
    16861 ctgattatga gcttcgatta taattttgat gccgaaaccc acctatcctc tccgcttact
    16921 gtttctcaga tcacttctct tataagaaaa gacttagagg gtaaataccc tgatgttatc
    16981 atcgagggag aggtttcaaa ctgtaagatt gcagcttcgg ggcatctcta tttttctctc
    17041 aaggatgaag atgctgtgtt acaggcagtc atgtttcgta gggatttgct gacccttgct
    17101 tttgtaccca aagacggtat gcgagtatgg gcacgcggcg ctatatcagt ctatgcacaa
    17161 agaggacaat atcagctcat tgcaagaagc atgagacaaa caggatttgg cgacattctt
    17221 gctatgctag aacagcgcag acgaaaattt gccgaggaag gcctatttga tgagtctaga
    17281 aagaaaaaaa tccctatttt ccctaccaga ataggagttg tgacgagtcc tacgggcgca
    17341 gcgatacgcg atatcattcg ggtgcttcat aggcgcaatc ctaaagtgag tattttgata
    17401 cttccaacat tggtacaggg acaagaagcg gcagcttcaa tagcggcacg aattcaacag
    17461 gcgaaccgct ggaatttggc agatgtgctc attgttggac ggggaggagg ttctatcgaa
    17521 gatttgctac ctttttcaga agaagtagtg attcgcgcaa tcgcagaatc gcacattcca
    17581 ataataagcg cagtaggtca tgagacagac tgggctcttt ccgactatgt ggcggattta
    17641 cgagctccaa caccttcggc tgcggccgag atggcatgta gtgatctaga tttaatatat
    17701 aaagagttca ctcattttga gaacatcttg gggcaatcga tgaagcatgc tcttgcgagt
    17761 gcgagacaaa ggctctcctc atcttctcct cgcattctgg aagacatcat actgcgaaaa
    17821 aatattgtcg caagccagag atttgatgct gctatcgaaa atataaggaa ttgctttcag
    17881 aaaagaatcg aagacctaaa tcatcgctta gttattgctt cgagcgccat agaaatagct
    17941 aatcctaagg cagtaatgaa acgtggtttc tcagttgtca agaaaaatgg gacagatatt
    18001 gtccgatctt ccagaactct cgcacaagga gattttatca atatcatgtt ctacgaaggc
    18061 gaagccgatg ctgatgtaag atcaatcaga aatgctagcg acaaaaggag tcagggatga
    18121 aagattttga aaaaaggatt acacatctcg aggaattagc agaaaaaatg agggagccgg
    18181 atcttccttt ggaaaaatca ttcagccttt tcgaggaagc tgttgtgctc gcccgagagc
    18241 ttaggaagga actagatgag cttcagggca aggttgagat tctcctcaat agccttgaga
    18301 ttagagaaaa cgatcaggct gaagtgccca acattgccaa cttcgatata tcgacttccg
    18361 aatctcaaat cgactcagaa gagaagtaag ggcaacaata agccttaatt acccaacttg
    18421 atggccgctt ccactattgg aattcatagc cgcttccact attggaatta gagaacgatc
    18481 ggaatcggcg agttccatct tcgaaatata ttctatagga agccacgcta cttcgacatg
    18541 ctcatttaga tgctggattt gtcctgaact aagcgagacc tgccaggcgg caagagtccg
    18601 agtctttccc ttgttctgga aaacgctctc gcctagaaac cttatcacat caatatcagc
    18661 gccaaactcc tcaaggaatt cgcgctttag agcagattcg tctgattcgc cttcttctac
    18721 ttttccgcct ggaaattccc agcggtggct catttccgta tgatcctgct tacgcctggc
    18781 aatgaataat agatcgcttt gaatcgcaat gcccgctact gatcggagat attcaatcat
    18841 atcggtagta tcatcttgtc cagacatctg tacttctcac tgctatctat catcatacac
    18901 attagtgtcc atgactccgc tacttatacg agcacaagca tttgcaaggt ctataggcgt
    18961 aaaccatccc tttttgagcc aaagctgttt tcctgctgag ccatgcacta atatcgcccc
    19021 taataatgct tttgtcatag gattatcaga agggatagcg gcatttgtcg attctcggtt
    19081 ctctcctgtt tcttttaaga aagctaacca ccgagcaaga aaacctccaa tcattccaga
    19141 caatacatct cctgaccctg caatgcccag cccaggtgtg cttccatcat atatgtaaca
    19201 agagccatct ggtaaaccta catgagttgt ggaggccctt agagcaagcg ttgttccgta
    19261 cttcttggca aatgctgtta ccaatgctac tgtctccgaa acattagcta aaggatctaa
    19321 aggaactgac ttcaaaatcg gaagcatctc gctaaattcg ccaggatgtg gagtcagaat
    19381 tatgggcgct ctacaaaagc tagaatcctg atttttgctt gcgatccttg caaacatccg
    19441 agcgccatct gcatcgagaa caagtggtat atctgtctgg aaaagcatag caagaattct
    19501 ctccctctcc aaatcgattc cccacccagg gccagcaatt acaacatccc agcattctgg
    19561 cgaaaatgag tccttcgaaa atacacgaac aatagcattc tctcctatag acgcgagata
    19621 ggccgaaaat aattctttat cacagaaaag ggcgatatat cctgccccag cagatgctac
    19681 cccatgtatg caatgcagag cggctcctgc tccgcttgag cttccagcaa acacagccac
    19741 tcgccctcgt tgcatcttgt atgcccatgg agagatcggg ggtaaaaagc tcttcatatc
    19801 actttcttcc aaaagccatg tattctgcgt cgatgacttc gtccattgag ggaaaatgcc
    19861 tccaactgga aaaatgtctc ctgcatatgg ccttgcgctt ggcatatata gcgactcttt
    19921 cataggctct atacagagcg taacatcggc acaaacgatc ggaaactccg gtttccatgc
    19981 atcactaagc ccagaaggaa catctaacga tacaatgaga ggattctttt tgcccatttc
    20041 gttgcgggat attcttatat ctcgaagaag tcggattaaa tctgaggact tccccttagc
    20101 tggtccatct attcctgttc caaacaaggc atcgagaata acatcatttg tggaaagctg
    20161 ttcactaatg ctttttgggt caactgtttc gtaacatatg atttttaccc cacatcgctc
    20221 tgcccgctgt aaatttaaga tgacatctgg cttcatggtg cagggttcgg gtatatatgc
    20281 agtgagccga gaataacctc ctaatcttgc atgccgcaac atggttaggg catctccagc
    20341 attatcgcct ttcccgcata cagcagctat gttaagctgc tgagatgaca ccgagtttgc
    20401 ggagattaga tttgaaatat cttttttaag aaatacttcg cttacaagag tctgccataa
    20461 gcgaattgca gcaatttcca tgagatggtc tacagaaaaa ttgaattcgg ttcttgttag
    20521 ggcatccagc tcaagagaat ttttattcga tatcaaagca tgcatatcgg gaggcctcca
    20581 atctactata ctctcacaaa tctcatcggg caagcaaaaa tctaaaggga tacaatgaag
    20641 tcttgcaaaa gtcagtgctt ttcgtatagc gttatagcat gaacgacttt gcgagtttag
    20701 gcataaatgc gaatctcata aatgctttag agatacttgg ctttcatgaa ccaacagaag
    20761 ttcaaaagca cacagtgcct ctccttctag aaaagaagga tcttagcatg caatcggaga
    20821 caggaacagg taagacgctc gcataccttc tacccatcta caattcgata ttgtcaaaca
    20881 ttgaggaagc taacagaaga tggccgatag cggctataat atgtcctacg caagagctgg
    20941 cagtacaagt agccaatcat tcgacccggc tagccatggc tctagaggca aaagataagg
    21001 gcgcaatatc ggaatcaggc tcgagccagt tgcaagaatc aagccaagga aacgctcgag
    21061 ctcctaaaac aatttcaaat cattcaaatt ctccacagat tcggagtctt gttctcttag
    21121 gaggaactca attttcgcgc caaaaacaag cgctaagaat gcatcctcac ataatagtag
    21181 ggacaccagg gcgccttgca gacctactac gcttacgcta tttcgatttt tctcatctcg
    21241 agtttcttat tctcgatgag gcagacaggt tgttttccag agaatatatt gagcctgtgg
    21301 agtatcttct ctccaatgct cctcggcttt gcacgcgcgt gatggcctca gctactattc
    21361 cagaacgaat ccgtaatagt gcaaaaccat ggatacctaa gcctatagta atcgaacttg
    21421 aaagtgaggg agtcttgtct caagccatcg agcattgggc attctattcc gatcacagaa
    21481 aaaaaataga tcttcttaag aaaataatag ttgccataca tccaaagcgc tgcctgatat
    21541 ttgcgtccga agcctatcgc gtccaaaggg ccgccgatcg tctgtgctct tctgggctgg
    21601 tatgcgctag ccttgtgtcg agaatggaaa agcaatctaa acattccata atagaacaat
    21661 tcaaaaccgg cagtattcct ctgctcataa caactgatct tgctgctaga ggcctcgata
    21721 tccctgatat aactcatgtg attagccttg atttgccaga agatggcgga gtatatgtgc
    21781 atcgagcagg ccgtacggca agagcaggca aaaaaggaat aagcattctg gttgcagata
    21841 agctcgaact tgaacgtgcc tcaaggaccg caacacgatt tggctttgtg tttcgtacaa
    21901 aatggcttga atacggaact gtggtggaac cttcggtcga agcctttttt gagagaattg
    21961 aagatatgga aacacaaaag cgctcgaaga gccatctatg agtgcttctg caattcatga
    22021 acctcaaatt atcacagaag ctgtagctgc cgatatggcc aatccgcttt tcgaggatgc
    22081 tcaatatagg attctaatcg taagactctc tccctttcgg gatgtcgaaa gctcatatac
    22141 ccatctggtg ctattcgatg aaacaaggcg cgctcttccc gccgcctata tcgattttgc
    22201 atttatgcca aatttgcccc ataaaaaagc cctttctatg gaaaacagac cttggttttt
    22261 agggcgcgca tccaatagaa gtgctttaga attcaatatg cttctaattt cttgtgcatt
    22321 ttccctcgaa ctattgaaca tcccttggct tctcactcaa tctggaatcc ctttttcaag
    22381 acaagaaagg atggaaaacg atagtctgcc ttttcttctt cttggaggat cgagcgcagt
    22441 ctgttctggt tcgctcgtaa aaatagccga taatagggta atcgatagcc ttgtcgatgc
    22501 tatgtttttt ggcgaaggag agggcagaat atcggagatt gtccgcattg cagccgaaga
    22561 ttcaagatca ggcttatcaa aatcgtcgat catcgcccat atagcttcag aaatcgaagg
    22621 gttctggccc tgcgattcga gttttgcctg taaacgagcg ctttctaccc aacgtccagc
    22681 agttcttacc tcgcctatta tgctaaatag tgagaatgca gatagcatca agctggcaat
    22741 tactgctgga tgcataggcc attgcacctt ttgtctcgaa ggctgggata gaagaccttt
    22801 tatcgaaaag cctgtcgacc atttatccaa atctgcaatt atgctcaaaa gagcaagtgg
    22861 cgcctcagat gtagagcttt tcagctacaa cttcaacatg cataaaaaca taattcagct
    22921 gatccagatt tttggcaagt attttatgca tgtctcaatg atgagccaac gcctcgatat
    22981 actatataaa aaacctgaaa ttcttgccgc ggaactagct gctgggaagc gctcttttac
    23041 tcttggcata gaaggaatct ctgagagaat tcgcaactac taccaaaagg gaatatcgga
    23101 agaacaaatt tggacttcta taacaagaat tctcgaaaat agagcaagag agatcaagct
    23161 ttttttcatt atttcaggat tcgaggaagg aagtgactta gaagaattcg cgcatttttg
    23221 cgatagacta gcccaccaca aaatcgaaac gcactcggtt acaagagtaa tcgtttcagc
    23281 tggctatctt gttaggttac cctttacacc tctacaattt gccccattgc aaggaaacag
    23341 ggctcttatg gagagcattg catctgccct ttgtaaaagt tgtaagcaag caaatctaga
    23401 atttcgacta gcgtcctctt tcgaggatta ttggatggat cagcttcttt cacttggggg
    23461 ttcgatagcg catgactggc tccaaacttg tcctaagaat gggtttttct atgatcttca
    23521 tgttcctagt cgagcattag agagcctttg tgcttacttt gagaaacagc ctatctttaa
    23581 ccaactattg gaagaaaaac ctaaaacata tcgccctgac ttttatttta tcgaaagcga
    23641 tagacactgg caaatgcttg cggcgcttta tgatcaatcg ctcaattatt tgcataatcg
    23701 agattctcgg aattcatata tagagaatca ttctggttcg tcgatctcta tagaagcaaa
    23761 aaaaaccata gacattatca aggcccaaca aaaagctaaa gcccatttcc cttcaatttt
    23821 aataaaaata agcgagaaca atgccttagc tttttcgact cctgcttatg aacgaacctg
    23881 gcttctgaga actttatcag ctttggtccc taattcggaa ctgggtttct tttattgtaa
    23941 ccttcagttg ccaaatttgg attgggaaag ttctatgcca ataagttctc catccttggt
    24001 ctatagatct aggcttggta tatcaggggt aaaatacttt gctatctatg gtccagatat
    24061 aactaatatg aaaaaagtca taagtcttac cgcaacggct ctaaagaatg tcagaggtat
    24121 tgaatcaatt tcgaattcaa gcgcctattc tggatcgact ctttttttgg aagatataga
    24181 attgattcag gagctcccga cagcaaaagt ttgtagactt tcggcttgta ttcctgcaga
    24241 taaaagcaga ttgatcaatg aggctcttga ggaatggctt tctgagatgg gtctacattt
    24301 tacattgaaa aaagatgaag atagtataat atattataca tcatcaatta ataagactaa
    24361 taaagctcta aaatatataa aatataagac aatatctacc aatatttgcc tttctttatg
    24421 tataggcaag aaggctaatc tacgactgct agcagatatt ctctataaaa aatgcaccat
    24481 cgaaaaaaca atatttcgaa tcgaaggctg ggatttgaat gccctcgatt tagatgatca
    24541 ttgagactct aaagcctttt aaatcttagc aaatcttaac tgcggctttt gaaaagacaa
    24601 aacctattga acttatagaa tataggtctg gtgtcaatta ttaagcatta ggtcattcgt
    24661 ctgggacaaa acgcgccatg gtgctgcttg tttcgaaaat gtctaccgct gagattggca
    24721 agcaaggaaa gccattgtgt aatcgagtat agataagagc agcaattctc tctgcacttg
    24781 gatcattttc caattctgct atctgattca aatcttgatg atcgaattcc ttatcaagca
    24841 attctcttaa tgcggacttg agtatgccaa aatcaattag cattccaccc tctccaagat
    24901 ctttacctga agcccaaact cttactttat agttatgtcc atgaaaacgc tcacactttc
    24961 cgttatagtg aacaagcctg tgagctgctg aaaactcggc ttccacccta atatgataca
    25021 ttttcccttc ctttgttgag ctcaaaataa tcctatttgt gatgctcaaa ttgcgatatc
    25081 tatattatta tatatgattc tttggaaaac atcttgtctt tgacccctaa tcaatgttta
    25141 ccccagagtt cattgcagct ctctgcagtc ctatgataga attcaaaaca atatgcagaa
    25201 acagctatca gacctaatca aaggactcga tatcagctca atagaaggag atgttcatca
    25261 tttgatatcg gatctttcct atgattcaag agaatgtaaa gctggatccc tgttttttgc
    25321 acttcctggc cttcatacag atggcaagga atatatcatc caagctatac aaaatggttc
    25381 ggttgcagtt gtctatgaag gcatgctaga gaagcgctta agggaatttc tgctgaaaga
    25441 accattgact aaagccataa atcccgcact gattcaagtc atagattgcc gatggacaat
    25501 gtctgcaata tctgctcgtt tctttgaaaa tccctctgat tccctatgcg ttattggcgt
    25561 aacaggcaca gagggtaaaa gcacaacggt atctcttatt taccaacttc taaatgcaac
    25621 aggtttcaaa gcaggattct tttcaactgt aatgtccgat attggagagg gagaaaaacc
    25681 aaatcctcaa catcagacaa ctcccgaagc aaccactgta cagcagatgt tggcagaaat
    25741 gcgcgaccat ggcctaggct ttgcagtggt agaagcctca agccatggcc tctctccacg
    25801 aactgcaagg cttgcccatg tgcatttcga tatcgggctt gttaccaatg taactcatga
    25861 acatctagaa ttccatggct catgggagca atatcgcagc gataaggcaa acctatttag
    25921 gcgcctcggt gaggctggag taaagaatct cgatagggtc gatcttaggg caaaagagca
    25981 taaaaaaatc agaatactct ctcctgttgg aataatctgc gccgatgatc caaatgcatc
    26041 atactttgct catgaatctg ctgctaattg tttgacatat tcgagcaaag gtgctccagc
    26101 atatctctca gcacataata taataggaga tcccaatggc gcctccttcg acatagaagg
    26161 gcctctagaa atggctatag ataatggcct tcaaccgaaa catataacag gttctcaagc
    26221 gcttaaagct gcaccgctac tgacattaca agcccgaatc aaccttccag gcaactttaa
    26281 tgttcaaaat gcgcttgggg caattctcgc tgcctccacc gcaacaggtt tgcattggcg
    26341 caattttgtt ccttttcttc cgtatctcaa accaataaag ggtagaatgc agcgaattct
    26401 tgaagggcaa actttcgaag ttataatcga ctatgctcat acgccatcct catttaaaga
    26461 aatactcccc actcttcgaa gagaaaggaa aggtagaatc atctgcgttt ttggatcggc
    26521 tggtgaaaga gatagagaaa agagaccacg gcaaggtcgc attgctgcgg attattgtga
    26581 catcttaatt ctcacggacg aagatcccag gggcgaagag ccaatggcta tactcgagga
    26641 gattgcttcg ggttgtcctg agctatcaaa agaagatcgc ttatatctca taccggatcg
    26701 accggcggca attcggaagg catttagcct tgctaagcct ggagatcttg ttttgcttct
    26761 aggcaaaggc catgagaatt ctattattta ttccgacaga actatcccct atgatgaaga
    26821 aacccaagcg cgttcgattc tagctgacat gggattggga ttaagagcga aaggaaataa
    26881 aacatgaaga acatagcagt gctatatggt ggaagatctg gtgagcatga agtgtcgctt
    26941 atctcagcga gcagcatttt agcctacctc gacaaagaga agtattcgat tgtgccaatt
    27001 ggaataacca aaagaggaga atggttcctc cagtatcctc cagagtgggc ttatttaccg
    27061 cctgaaaaag ggcaaatgcg ctctcttcct cctgtcgaaa aaactgaacg cgtcttagct
    27121 gtccctggtg aaggactctg tctagaaacc aagaaaggag tgcaaaaact cgatattgat
    27181 gttgtatttc ctgtgctaca tggaactttt ggcgaagacg gtacgatcca gggtttacta
    27241 gaaacagtcg atcttcctta tgttggcgcg gatgtcttgg ggtcggcggt ctccatggac
    27301 aaggaagtat ctaagcgctt atggctgtcg gcaggtctgc cagttgtcga ttatatatca
    27361 gtaagaaaag aagatgtctc agatgaaaac ctaaagatac ttgcacgaag agtggaaact
    27421 agatttggct ggccatgctt tgtaaagccc tcatgctcag gttcctcggt cggaacctct
    27481 aaagtcaata attctgagga gctaaaaaac gcaatactct ttgctcttcg ctggtctgag
    27541 catgcattga tagaggcatt catcaatgct cgagaaattg aatgtgcagt gcttggtaat
    27601 gagcgacctg ttgtttttgc tccaggagag ataattccat ctcacgaatt ctatgactat
    27661 gaagcaaagt ataaggatcc acagggcgct acccttttaa taccagcctc gctatcggaa
    27721 gagacaaaga atcgaataat gagcctcgcg ctttctgcat ataaatatgc atcgataaaa
    27781 ggtatggctc gagttgactt ctttgtcgag aagataactg gcgaggtgtt tataaatgag
    27841 atcaacacta ttcctggttt tactgccatt tcgatgtacc ctcgaatgtg cataaatgga
    27901 ggagtctcct atattgaact gctagataag ctcatagagc ttgcattaga acaatatgca
    27961 ctaaagcgcg aactcgaata tgacttctca aaatcataga ataactgagc ctctttcaaa
    28021 agtctaaaaa tattctggca tgaattgtaa gaataa
//
LOCUS       DAOSOB010000020        27950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1710_length_27950_cov_65.539882, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000020 DAOSOB010000000
VERSION     DAOSOB010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 27950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27950
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1710_length_27950_cov_65.539882"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1334
                     /locus_tag="PLS88_03545"
     CDS             183..1334
                     /locus_tag="PLS88_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOU06455.1"
                     /translation="MPARGNQWSRTSARIFRTIWRNPSISRVDLARKLDLDKSTVTKQ
                     VAYLIENGLIMEQKEGEASARGGRKPIYLAVNKEYGRFIGIELQLGFYEAVVVDLSGN
                     ILAALRGDTHVTSDNFVQVVLQIIEDTRKQHCKNSLLLGVGVGTAGLVDSKKGRIRYS
                     VPLGIDKTLDFSKVIAGKLDVPCFIENDANCCAWGELAFNRVEELRDFLFALVEYRKD
                     ETSLLKYGGIGVGFGVVLAGKVHTGAHGYTGEFRSAFCNGPGNLQFSLSRDQLMLLRF
                     DRSLLDRAVDELARNMAMIINTMDFQQVIIGGNIEALDVDLPAILRRRLEENWMYPSP
                     KDVKIRYSSFGEKAVAFGAAGMVFERLLSEQRLPGLVSTNSSDKMKRIS"
     gene            1422..2726
                     /locus_tag="PLS88_03550"
     CDS             1422..2726
                     /locus_tag="PLS88_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU06456.1"
                     /translation="MKKALLVFLTLMMVLGAVVAQEKVTLNVLNYQQADQAGYQEDVA
                     IWEKFQKENPDIKLNIEVLFSEPYHQKLQVYAAAGTLPDVFYVWPTSRSAIIHEKKLA
                     KDLSKLLGPDYLKDFSGAATDPNNQLGKYLAMLPQAFTYTSVMFVNKKLLTDNGFDLP
                     KTYDDLKRMVWKLKAKGINVIMLPNKDGWPMQSCLFSTVLGRMAGDDFVDAILAGKAK
                     FTDKPFVDSLKVIDNLYKDGIMAREDNQVGYGEGPGLFVSGKAAIYIDGDWRAGAYIT
                     DKASGKALIPPAAQEKDFVLLPFPAIPGEVNPGVLSGIAGTGWAISASIPSGSAKEKA
                     AVKLFKYLYSEEVQSIRYSTGSYVPTRKNVKANVEPLVAKVPQFYADNPKVCYVFDGM
                     FDPAVYNVLNDGLIAIGLGTATPEKVAADVQKAFETWKASAK"
     gene            2784..3947
                     /locus_tag="PLS88_03555"
     CDS             2784..3947
                     /locus_tag="PLS88_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU06457.1"
                     /translation="MSSTLSAPFWGVFMTYSSKSYIEEKRAYWAMILPAFTIYLLVMA
                     FPIVLSIVLSVSNYSGGKMFGGEKWGFAGFDAYLRVFRDPWFWNALKNNIYIVLVSVF
                     GQLPLGFIFAYFIYARIVKAPEFWQGVLYVPNIISVIVVGLLWQTIFSPHGPIAEVIN
                     SIRVREFQNQVRGIFNAAGGFSLSDDVIQKILDLVGPSGQAIFSDPLIDLRDLLSSFH
                     GEPIEEVYNALGNLFVQKWSPTFLTKSNIAMLPILFVILWMYTGMYLILFLANMQKID
                     VQIVESARIDGANEGQVMRYIILPALSGTIVNAAILAISGSLSSFALVFAMTGGGPSR
                     VTEILSIYMYNNAFLGRPNFPLANAISLIMVLISFGLILLTKAVEKRYGGKEE"
     gene            3960..4811
                     /locus_tag="PLS88_03560"
     CDS             3960..4811
                     /locus_tag="PLS88_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU06458.1"
                     /translation="MIKESKSKKILSIIGKAAVYLIMGIFAFMTLYPIFWLIMNSFKT
                     TREFQVSQLALPKSPTLQNYVGAWQIGDFSLLFPNSILYTLGATVGIIFLSLLAGFAF
                     AKLKSKATKPIYNSFIMGILLTTQTLMIPLFLEVNLLGIYNTRFAVLLVYIGAGLPIG
                     IYLSTEYIRAIPSAVVESARIDGAGFFRIFLSIIVPMSVPVATTLAILNITNTWNEFA
                     LINILVSKTELKSLPLGIYKFSGSLSSDYGKQFAALTIGMVPMLAFYIIFRKQITKGV
                     AAGAIKG"
     gene            4839..6179
                     /locus_tag="PLS88_03565"
     CDS             4839..6179
                     /locus_tag="PLS88_03565"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOU06459.1"
                     /translation="MKREDGFPQDFVWGCSTSCYQIEGAAKEDGKGPSIWDTFSHTPD
                     KIAGGHTGDIACDSYHRWREDIALLRELNAGAYRFSVSWTRIQPEGKGATNQRGLDYY
                     SRLVDELYEAGVEPWIELFHWDLPQALEEAGGWRNRDTAMRFQDYAAIMYAHIGDRVR
                     HWTSMNEPWCAAFLGHLTGEHAPGLRDRRAANRAVHHLLLAHGLATRAFRRSGIKGEY
                     GIVINPSKPRPATLRPEDVEASERASIERTSLWLDPVFGRDYPKAFMDIFGSDMPIEA
                     GDMDIIAEPLDFIGVNYYNEEAVRAAPFNAENPYGFEFVPTYQKKTEMGWEIEPSGLH
                     RILTYIATLWPCKKMYVTENGAAFLDKPDPDGIVRDYERIDYIRSHANACRDAIKQGV
                     PLKGYFLWSLIDNFEWSFGYNRTFGIATVDSNTLERRPKLSFYYYRDLIAGFGL"
     gene            6189..7790
                     /locus_tag="PLS88_03570"
     CDS             6189..7790
                     /locus_tag="PLS88_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOU06460.1"
                     /translation="MSELSSIIPAPVACEPGEGSFRFNQETCIATEPGLEKEAGVLAA
                     WLSRVPGIERVKIVSGNSDNHKPLAIPSEPGSNKTATIRLSLESALCLKTTEAYVLEI
                     TPSSIQITAAEGAGIIRGAASLWQLVLGKSCEEGQRVSIQAGRIVDKPRFGWRGLMLD
                     CARNFFRVEFIEKLLDLAAFHKLNVFHWHLTDDQAWRLDIPSRPELVEYGAFRKDQRY
                     QGECKKGGYYSQSDVKRVIDYAQARHILVVPEIELPGHSIALFSSHPEFSCRGRADSS
                     VHFEPEDRYGIFEDILCAGNDDVFTFFDNMLQEVYKMFPGPYIHMGGDEAPKDRWLEC
                     PACRERMQALGFASGDGRYFPEKLQAWFMGRISNLLVNHGKRMIGWDEVVDGGIGKDT
                     IVMSWRGIEPGIHAAQCGYDVVMCPQTRACYLDHKHNDDPEEPGHLGVCTLKDSYLFE
                     PVSERLSDEEKHHILGGQANLWTELIYFGRQAEYMLFPRLCALSEVFWSPREMRNFED
                     FSRRLRLHEHRLDTLDVSYYKGRFF"
     gene            7923..8099
                     /locus_tag="PLS88_03575"
     CDS             7923..8099
                     /locus_tag="PLS88_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06461.1"
                     /translation="MSGFFTIHNSRFFNKYAERIQRFEVSMRFKDFQDFEDAQYFEFI
                     EANKAPIISSLTIL"
     gene            8133..10337
                     /locus_tag="PLS88_03580"
     CDS             8133..10337
                     /locus_tag="PLS88_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06462.1"
                     /translation="MRTVLRYLKPYWLDIVFIVVLLFIQANADLSLPDYLSRIVNVGI
                     QQNGIEANLPAVMQKSTFEALGRLFQMEAKPEDFLALQKVYVEVLPDSPQAQSLSKKW
                     PLAKKEPVMMLSSKNKAELDVARAIFLSEYPKLAAFLQFSSMAAGKVSPSPQMSKSGL
                     SGLSSMTPQELSAQIEALDPLIKSQIVVLGLQGEFTALGADINAMQSSYILRVGIQML
                     LITLIAVGSAVLVGFLGSRLSAGTSRDLRNAVFSKVEEFSLAEFDSFSTASLITRSTN
                     DINHIQMLIMMSTRMLFYAPIIGIGGVIRALSKASGMWWIIAMAVGVLLGIVGLVFAL
                     VVPKFRIVQKLVDKLNLVVRENLSGMMVIRAFNMQDHEAKRFDKANRDLTSMMLYVTR
                     AMVVLMPLMTIIMNVVSISIIWVGSHEVAVGSIRVGDMMAFMQYSMQIFFAFLMMSAM
                     FILFPRASVSAERIAEVLNKEVSIRDPEKPIHLSKPVRGEIEFHHVDFKYPGAQENVL
                     EDINFIAKPGTMTAIIGTTGSGKSTLVSLIPRFYDVTGGQILLDGIDIRNLPLAELRS
                     QIGFVPQKSMLFSGDIEENIRYGKEDALESEIMKALEVSQIMNLVEESPEGLKRPISQ
                     GGMNVSGGQRQRLAIARALVRKAPIYIFDDSFSAIDYKTDRAIRSALKKYAADSVIFL
                     VTQRVAPIRNAEQIIVLYEGQIVGIGIHEQLMKTCEVYRDIALSQLKQEELV"
     gene            10334..12283
                     /locus_tag="PLS88_03585"
     CDS             10334..12283
                     /locus_tag="PLS88_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06463.1"
                     /translation="MNNNERQEQLNNSSAFHETQMQRRPGAKPVRPGRRALMAGEKSR
                     DFKGTMKKLIAYLGPYHWPILCVMILAIVSTILAILGPKVLGNATTALFEGVVKKLTR
                     TGDIDFAKIKTILLTVLVLYVSSSILQYLQGWVLSGIAVKITYSFRKDILAKINRMPF
                     KAFDNTNHGEILSRITNDVDVVNQTLTQTISQMIHSIMTIIGVFVMMLTISWQMTIVA
                     LVTIPLSLIVVRVIVKRSQKYFRIQQEYLGHVNGHIEEIFSSHIVVKAYGGEERAIQT
                     FNELNSTLYQSAWKSHFLSSIMMPLMNFVGNIGYVAVAVSGGWLAAKKMVTVGDIQAF
                     LQYVRQFTQPIVQIANISNVLQQTAAAAERIFDFLASEEEKDVSIEMGQKGALVEIPE
                     SIKGKVEFRHVCFGYLPDQPIIKDFSATILPGQKVAIVGPTGAGKTTLVKLLMRFYDP
                     DSGEILVDDIPIRSLPRSLVRSWFGMVLQDTWLFSGTVKENIAYGKRDATEAEIVEAA
                     KAAHVDHFVRALPEGYNLEINEETTNISQGQMQLLTIARAMLAQPPMLILDEATSNVD
                     THTELLIQRAMGLLMQGRTSFVIAHRLSTIRDADLILVMDHGDIVEQGTHEELLNKGG
                     FYAELYNSQFDQNGEDRLAISASSF"
     gene            complement(12411..14048)
                     /gene="groL"
                     /locus_tag="PLS88_03590"
     CDS             complement(12411..14048)
                     /gene="groL"
                     /locus_tag="PLS88_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710555.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOU06464.1"
                     /translation="MAKQLLFSEDARRKLLVGVETISEAVRVTLGPKGRNVLLDKKFG
                     APTVTKDGVSVAKEIELEDPYENMGAQLLKEVATKTNDIAGDGTTTATVLAYSIVKEG
                     LKSVAAGIDPMALKRGIDQAVTLSVQEIRKISKDIKEKEEIAHVASVSANNDIDIGNQ
                     IADAMEKVGKDGVITVEESKTMETTIEYVEGMQFDRGYTSPYFVTNRDSMTTVFENPL
                     ILIHDKKISNMKDLLPILEKIAQTGKPLLIIAEEVEGEALATLIVNHLRGTLNVCAVK
                     APGFGDRRKAMLEDIAILTGGEVISDELGLKLENTSLSQLGTAKTVKVDKDNTTIING
                     GGKQKDIQDRIAQIKMQIEETTSDYDREKLQERLAKLAGGVAVINVGAATEVEMKEKK
                     HRVEDALSATRAGIEEGIVPGGGIALIQAAIALDKADTSKMTDDERVGFKIVKRALEE
                     PIRQIAENAGIDGSIIADKAKQEKKGIGFDAAKLEWTDMTKAGIIDPAKVTRYALQNA
                     ASVASLLLTTECAITDLPEKNPPAMPAPNAGMGGMEY"
     gene            14326..15057
                     /locus_tag="PLS88_03595"
     CDS             14326..15057
                     /locus_tag="PLS88_03595"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU06465.1"
                     /translation="MKEICQILYEDQDLLAIDKPAGMLAIPDRWDTNVPVAQQLLQNQ
                     FGKLMPVHRIDKDTTGVLLYARNEAAHRIISNDFSNRKVEKIYFAIVQGEPESDTWEV
                     ELPLRADGDRLHRTIIDASKGKEAKTRFEVIERFRGFSLVLAIPETGRTHQIRVHLAA
                     SGLAIVADPLYGNGAPLLLSMLKAGWKGDAYEERPLIDRTALHSAKVSFVHPKTKEGL
                     IIEAPFPRDFKATLAQLRKVRTRWA"
     gene            complement(15072..16442)
                     /locus_tag="PLS88_03600"
     CDS             complement(15072..16442)
                     /locus_tag="PLS88_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU06466.1"
                     /translation="MPLQKSDLSDAVINTYYAVRGPIVARAQQLENTGKEIIYCNIGN
                     PQALKQKPLTFVRNTLAACEQPELLKSPNIDLMEDAKEKAHYILEQSKHGLGAYSESK
                     GLRFVRKAIADFIISRDSFNGVEQNADFENIYLTDGASKGVQAALTLLIAKKNDGILI
                     PIPQYPLYSATITLYGGQQIGYYLDEESGWSLDEQMLEDAIQQALAKGIHARAIVVIN
                     PGNPTGGVLTENNIRMVIHFAKRHNLTILADEVYQENIYKPGARFISFAHVMTSMKEK
                     EVSLFSFHSVSKGFFGECGHRGGYMEVRNVPPEVIDQITKLQSISLCANLPGQILAYL
                     MVSPPKPQDPSFQQFADERSAILTELAKRAKMLEEGLNSISGYHCQPIYGAMYAFPSI
                     TLPPGKSDDDYCMALLEQTGVCVVAGSGFGQKPGTAHFRTTILPPTEQIEKVIDALER
                     FHRTWK"
     gene            16562..17866
                     /gene="hflX"
                     /locus_tag="PLS88_03605"
     CDS             16562..17866
                     /gene="hflX"
                     /locus_tag="PLS88_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOU06467.1"
                     /translation="MTEVKRISEASRVFLIGVASGEMPKPEAKELLDELHRLTDTLGQ
                     EVVGEMLILLREYTSSLLVGTGKAQEIVDAANKADADTIIFDHVLSPVQQRNWEKLSS
                     KKVYDRAELIIKIFASRAFTKEASLQVELAQLEYTLPRLAHSYNDLMQQRGGRYGTKG
                     SGEQKLELDRREIERRIHEIKGELETVRKERAVQRKRRERSGVPRAAIVGYTNTGKSS
                     LLNALTSASVPAEDKLFATLDPTTRRIQFVRGQTLLITDTVGFIRNLPPGLVEAFHST
                     LEEASLADVLIHVADASDKNIDIHIETTMKVLSDIGAGEIPYILVLNKIDLVDLDTVQ
                     AFLSRYPGSVAVSAKTGEGLGELVNEIQEALTKQMEPLILRIPHNEYHIVSLILREAT
                     VLDEHYDEEFTWISCRIPRKLINAVQEYAYNGAFPLVDSNKP"
     gene            17898..18824
                     /locus_tag="PLS88_03610"
     CDS             17898..18824
                     /locus_tag="PLS88_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOU06468.1"
                     /translation="MKEIVSVAVVGAGAIGASIAVRLKDAGKQVIISANGERKDRYLK
                     QGFVVNGQQHFLPVRDQQEAAPVDLIIVAVKNYSLEEAIEEMRPYINANTIILSLLNG
                     IDAVPRLRKEFGEDRVPYAMILGIDALREENRVQYLAKGKIFCGFEKEKAQKNAPKLA
                     LLEEFFSTSDITFVVPEDIVKEIWFKFMINVGLNQWSALIRSPYRLFQNSLHGQELLR
                     QTMMEVIALSSHFDGNLDKTDIDRAIAVLQTLAPEGKTSMLQDVEARRQTEVDAFAGA
                     MMRLAKAIGLDVPINAILYEAIRAIEDSYKSQ"
     gene            complement(18807..19670)
                     /locus_tag="PLS88_03615"
     CDS             complement(18807..19670)
                     /locus_tag="PLS88_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOU06469.1"
                     /translation="MKNNLRSFFSTKPSSIKILSYASLDSTMDEARRIIRNGLDNDIT
                     IVRADTQTAGRGRIEGRKWTDTPKHCLLMTIILPKNYKGIVAIPLRAGLGVVRALETI
                     LENQALETRVSSRLTANHDQFERKREPYFFLKWPNDIVVLLPSNSCHFGKLCGILCEQ
                     IIDNVLIGIGLNLLKSSYDGLQTDLSPFSLEEALGFIPQAFSNLDSAACSITEHVLQA
                     LHDPNWHSEYEYRLWGLGTPMHFLIGHPQLAQEAEGICEGIDENGSLILSNNGEKRTF
                     VSGEISLLRLV"
     gene            19839..20858
                     /locus_tag="PLS88_03620"
     CDS             19839..20858
                     /locus_tag="PLS88_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU06470.1"
                     /translation="MKHDAWRVLPIVVFAVALLGALAFNIALVSRSRNALASTILRNP
                     STIHSARFHIIAILPDTVDPFFTHLIEGLSEEAEKQDAALQIFYFSPTGAIDSGSISG
                     EVLRWFEIALRSKTDGIILFQTAGLDVKKLAEAAENAQIPFVPMAMDVSQQWINAGIT
                     GDSASQGREASTLALSLLGAAARIGIILSSDTSLGYAFYEEPFYRGVEETLKSKQGAA
                     IVAAEREEESILGGEDACARMLAEHPNINAILCIDAKATIGAAQVVIDRGMVGQIVII
                     GADENSEVNRLIEKGVVQASIVRDAVAMGRAGVALAIGQRIGIRAPERISVGYRVIPA
                     RGGGL"
     gene            20855..22369
                     /locus_tag="PLS88_03625"
     CDS             20855..22369
                     /locus_tag="PLS88_03625"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOU06471.1"
                     /translation="MIDFALISRPLKARRHKEKGLRDRIIANSAVILLVLVIATSYTA
                     STSFNLARSVELLFHNSVSMEELRTVLRETQDNLTGYLSAKNSESLREFILNSTLLSN
                     MAHKLNKENKNDDVFLLEKDLACLVESYLASAEGAVQAKRGRDVTKYVSLYEEARRGA
                     SYIEFLSTRMDTIYLGQSLTGFSSYRANISLAIMSNGVLLLAAFLLSIALIGRYSYTI
                     TEPLARLSVAADAVGRGEYDHPLPPYEREDEIRTLHDAFVNMKDSVQKAFVELTRKAE
                     LEHSLMEERMRVLTYQHRLKDAELLALQTQINPHFLYNTLAAGWQLALSEGNEKTAEF
                     LEKLAAFIRYSLKPSTRFVLVSEEIECVRQYIWLLRLRFGERFEFCLDIQDEALGYET
                     PALVLQPLVENAIVHGLKDVEEGGKVIISAKLEGETIRFSVSDSGCGMSSEYITLALA
                     AARPDDTRPQNGIGLHNVVRRVALATGGLGRVEIESKLGHGTCVTILLPTGKTS"
     gene            22366..24006
                     /locus_tag="PLS88_03630"
     CDS             22366..24006
                     /locus_tag="PLS88_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU06472.1"
                     /translation="MKRVLIADDEIPIVNGLQLLFKRYFQSGYLVVGTAQSGREVIEK
                     SKTLAPDIVLMDVQMPGINGLDAIRELSQQGGTKAFILVTAYERFDIAREALSMGVCD
                     YLLKPVSKERLEIALQAASSYLDRLHILEEKELEFKEHQQKLLPFVKTAFFVYLRQQR
                     GAKDREPRETKRTKALRDELNLYKEVLHISGEAGVMGIASFSPADGLANSLYEYFCSA
                     LQYKTIALAGPLEDNRYCAWFLPLKSKMDQGTAAEAEVTAFLELLSSSFSSQLATGEL
                     RFACGQPELLENIGCSWDNAVRSFGLSSEYIISSRHRFEEDIIEDREEPASAFDAQFY
                     DAIMEGQYAMAGQLLEKLILTVEEAEAKPRDLYFRIIAALSFWASKLAGGGVLSDNVY
                     RDFMDFSDIEKLWEERFWQLFASAVRSRFQSLQKHAAEIGTHSPFVVKALQYIENNYQ
                     EPITLESAAAAIGISAGHLTRLMSDELKRGFARTLIGYRIQKAKELLKMPNISIREVS
                     QRCGYSDANYFARLFRRLTGLTPREYAARMAKGENSDA"
     gene            23999..25054
                     /locus_tag="PLS88_03635"
     CDS             23999..25054
                     /locus_tag="PLS88_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU06473.1"
                     /translation="MPKTMYKAHSHVFFMMTIILATITLSYFSCSKSEKTVETKSPLI
                     GFSLDSLVVERWRRDVDSFTKTAHDLGAEVVLRVANQNAETQIAQVRELLGQGIDVLV
                     IIPNDAERLSEVCREAKRKGIKVLSYDRLVHRANADLYISFDNEKVGSLQAQALVEAV
                     PQGNYVIINGATTDNNAFMINKGFHSVLDPFIESGKIHLLEEIWPSDWMSDEVRTRFE
                     ALAKPGQKIDAVLCGNDMLAETVISVLSENRMVGKTKVTGQDAELSACQRIAEGSQFA
                     TVYKPIDSLALKAAGFAVMMANGEKIRYENKIEDGSYKVPYIALEPVLVTAKTLESTV
                     IEDGFHSRDDVYRNVKH"
     gene            25302..26384
                     /gene="chvE"
                     /locus_tag="PLS88_03640"
     CDS             25302..26384
                     /gene="chvE"
                     /locus_tag="PLS88_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU06474.1"
                     /translation="MKRTLLIAMLFTVVAGFVFAETQVGIVLPTKDEPRWIQDQTQFL
                     DALKTAGFSAKVLFSQGDSAKEKANVEALVSEGIKVLIICPHDGTAAASAADLAHSAG
                     VKVISYDRLIRDTTSVDYYVTFDSFEVGKAWGDYLNSKVPAGTKGNNLYLYAGAASDN
                     NSFIFFEGAWSALQPKIADGTYIVRNSDKAVALAKKAKLTRDEEAQIIGQVTTNWNFS
                     DAKNKAEANLTAAPKEAKGTVYICAPNDGTARAIADAFAADKDVKTYYITGQDAEIAS
                     IQYIIDGKQSMTVLKDVRTLVKDAINAATAYMKGQTPPVTSYYNNGKKDVPAKPTAIV
                     TVTKENVKKEIIDSGYWPADKFTGLK"
     gene            26489..>27950
                     /gene="gguA"
                     /locus_tag="PLS88_03645"
     CDS             26489..>27950
                     /gene="gguA"
                     /locus_tag="PLS88_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU06475.1"
                     /translation="MNDNILEMRHITKSFPGVRALNDVNFAVRRGEIHCLVGENGAGK
                     STLMKVLSGVYPHNEFEGEIILNGKKVAFDGIHDSEEAGVGIIYQELALVPEMTVYEN
                     IMLGHEIRRGITVDVHEMIKKAEELLKRVRLSINPTAKIKDLGIGKKQLVEIAKALSR
                     NVSLLILDEPTATLNEDDCENLLDIIRMLKMQGVTSIMISHKLREVLSIAETITVLRD
                     GQTICTLDRRNDEVNEQVLIKHMVGRSIENIYPPRISKPEETISLEVKNWNAFDPKLG
                     RKVLNNVNFYVRKGEVVGLSGLVGSGRTELARSLFGNPDKYEISGDLILKGKKQRFSH
                     PSQAIKAGLAYATEDRKANGLILIKSIQENISLANLQGVSKNGLINEELDKQTALGFV
                     DSLSIKTPGIHQLVMNLSGGNQQKVSVAKWLFVEPDVLIFDEPTRGIDVGAKYEIYNI
                     INDLVAKGMSILMISSELPEILGMSDRIYVVANGTIT"
BASE COUNT         7745 a         5892 c         6667 g         7646 t
ORIGIN      
        1 ctttttacct cctatttatt tctgcgtctc ctattgaatt ctgaaagatt tttattgaca
       61 gttcaagata ctggtgttat atgttaattg gttggataaa tcaaccaacg ataatctaaa
      121 acctcaagct tgttcgacaa aacaaagcca ggtttcttgg ttgatgttga ggagataaga
      181 gtatgccagc gcgtggaaat caatggagcc gtacttcagc tagaattttt cgcactatat
      241 ggcgcaatcc atctataagt cgcgttgatc ttgctcgtaa gctcgacctc gataaatcca
      301 cagttacaaa gcaagtagca tatcttatcg aaaatggctt gataatggaa caaaaagaag
      361 gggaggcttc ggctcgagga ggacgaaagc ctatctacct tgctgtaaat aaagaatatg
      421 gacgatttat tgggatcgag cttcagcttg gattctacga ggcggtagta gtagatttat
      481 cgggcaatat tcttgctgct cttagagggg acactcatgt tacttctgac aattttgttc
      541 aggtagtgct gcaaataatc gaagatacac gcaaacagca ttgcaagaat tctcttcttt
      601 taggtgttgg agtaggcacc gcaggcctgg tcgattcaaa aaaaggtcgc attagatatt
      661 cggttccgct tggtatcgat aaaacactgg atttttccaa agtcattgca gggaaattgg
      721 atgtcccctg ttttatagag aatgatgcaa actgttgtgc ctggggcgag cttgctttta
      781 atagagttga agagcttaga gattttctct ttgcgcttgt ggaatacaga aaggatgaga
      841 cttctctttt gaaatatgga gggatcgggg ttggctttgg ggtcgttctt gcaggaaaag
      901 tccacacagg cgctcatggt tacactggtg aattccgaag cgctttctgc aatgggccag
      961 gaaatctcca attctctttg agtcgagatc aactcatgct tttgaggttc gacagatctc
     1021 tgctcgatag agccgtggat gaactcgcgc ggaacatggc tatgattata aataccatgg
     1081 attttcagca agtcattatt ggtggaaaca tagaagcgct cgatgtagac ctgcccgcaa
     1141 ttttacgccg taggctcgaa gaaaactgga tgtatccttc gccaaaggat gtaaagattc
     1201 gctattcgag ctttggcgaa aaagcagttg cctttggcgc tgctgggatg gtttttgaac
     1261 gcttattatc ggagcagcga ttgccaggac tcgtttcaac taattcttca gataagatga
     1321 agcgaatctc ctagtaggcg ttgagctatc aagggtaaaa aggctgatat gccttttcat
     1381 atcggaacaa gggcagaatt gcccagtata aggaggtttt tatgaaaaaa gccttactcg
     1441 tgttcctaac acttatgatg gtgctaggcg cggtcgtagc gcaggaaaag gtcacattga
     1501 atgtactcaa ctaccagcaa gccgaccaag caggatatca ggaagatgtg gcaatctggg
     1561 agaaattcca gaaggagaat ccggatatta aattgaatat cgaggttcta ttcagcgagc
     1621 catatcatca gaagctacag gtgtatgcgg ccgctggcac acttcctgat gtattctatg
     1681 tttggcctac ttctcgaagt gcaattatcc atgagaaaaa gctcgccaag gatttatcca
     1741 agctactagg cccggattac ctaaaggatt tttccggtgc agccaccgat cccaacaacc
     1801 aacttggtaa atatcttgcc atgctgccac aggctttcac ctatacatct gttatgtttg
     1861 tcaataaaaa attgctcaca gataatggct tcgatttgcc aaagacctat gatgatctca
     1921 agaggatggt atggaagctc aaagccaagg gtatcaatgt gataatgctg ccaaacaagg
     1981 atggttggcc gatgcaatcc tgcctattct ctactgtgct tgggcgtatg gctggcgatg
     2041 actttgtcga tgctatcctc gctggtaaag ccaaattcac agacaaaccc tttgtagatt
     2101 cgctaaaggt catcgataat ctatataaag atgggatcat ggcgcgcgaa gacaatcagg
     2161 taggttatgg tgaggggcct gggctctttg tatctggaaa agcagcgata tatatcgatg
     2221 gcgactggag agcaggcgct tacattaccg ataaggcttc aggcaaggct ctaattccgc
     2281 cagctgcaca agagaaagat tttgtgctcc tcccattccc agctatccct ggagaagtaa
     2341 atccaggcgt tctatctgga attgcaggca ctggttgggc gatttcagca tccattcctt
     2401 ctggttcagc aaaggagaaa gcagcggtca agttattcaa atacctctat tctgaagaag
     2461 tacaatcgat tcgctatagc acaggatcat atgtgccaac gcgaaaaaat gtaaaggcga
     2521 atgtcgaacc ccttgtcgca aaggtaccgc agttctatgc cgataatccc aaggtttgct
     2581 atgtattcga tggcatgttc gatccagctg tctataatgt tctcaacgat ggcctcattg
     2641 ctatcggcct tggaacagca accccggaga aagtagcggc ggatgttcaa aaggctttcg
     2701 agacctggaa agcttccgca aagtagaact atcgaaaaag taggggtcgg gaagcgtaga
     2761 taccttggca tggaggagcc tctgtgtctt ctacgctttc cgctcctttt tggggagtct
     2821 ttatgaccta ttcgagcaaa tcctatatag aagaaaaacg ggcttactgg gctatgattc
     2881 tgcccgcttt cactatctat ctcttggtca tggcattccc tattgttctc tctatcgtgt
     2941 tatcggtatc gaattattct ggcggcaaga tgtttggagg cgagaaatgg ggattcgctg
     3001 gatttgatgc ctatttgcgc gtattccgcg atccatggtt ttggaatgct ctcaagaata
     3061 atatctatat agtgcttgtt tctgtctttg gtcagcttcc gctgggattc atctttgctt
     3121 atttcatcta tgctagaatt gtaaaggcac ctgaattttg gcaaggtgtg ctttatgtgc
     3181 ctaatatcat ctctgtcatt gtggttggcc ttttgtggca aacgattttt tctccgcatg
     3241 ggccgatcgc cgaggtgata aattcgataa gggtcagaga atttcaaaac caggttagag
     3301 gcatattcaa tgctgctggt ggtttttctc tctcggatga tgtaatccag aaaatactgg
     3361 atctcgtagg gccttctgga caagcgatat tttctgatcc tctgattgac cttcgcgatt
     3421 tattatcttc attccatggc gaaccaattg aggaggttta taatgcgctg ggtaacctct
     3481 tcgtgcagaa atggagtccc actttcttaa caaaatcaaa tatcgccatg cttccaattc
     3541 tctttgttat tttgtggatg tacacaggaa tgtatctcat ccttttctta gcaaatatgc
     3601 agaagattga tgtgcaaatc gtagagtcgg ctagaatcga tggagcaaat gaagggcagg
     3661 ttatgcgata tatcattttg cctgcattat cggggacaat cgtaaatgca gcgattttgg
     3721 ccatttcagg atcgctttcc agttttgctc ttgtctttgc aatgactggc ggaggacctt
     3781 cacgcgttac tgagatactc tctatttata tgtataacaa tgcgtttttg ggtaggccca
     3841 actttccgct cgccaatgcc atttcgctta taatggtctt gatcagtttt gggctaatcc
     3901 ttcttacaaa agcagtagaa aagcgctacg gcggaaagga ggaatgagca atgcccgaga
     3961 tgatcaagga atcaaaatcc aagaaaatcc tatccataat cggtaaagcc gcagtctatc
     4021 tcattatggg catttttgcc tttatgacgc tctatccaat cttctggctc atcatgaatt
     4081 cgttcaagac aacgagagaa tttcaagtaa gccagcttgc actgcctaag tctcctacac
     4141 tccagaacta tgtaggcgcg tggcaaatag gagattttag ccttctattc cctaattcaa
     4201 ttctttatac tcttggagcg actgtgggca tcatctttct ttcacttttg gcaggatttg
     4261 catttgccaa actcaagtcc aaggcaacca agcctatcta taacagtttt ataatgggaa
     4321 ttttgttgac aacccagaca ctgatgatcc ctctttttct cgaagtgaat ttgttaggca
     4381 tatataacac gcgctttgcg gtgcttcttg tctatattgg agctgggttg ccaattggga
     4441 tatatctgtc cactgaatac attagagcaa ttccaagtgc cgtagtggaa tcggctcgca
     4501 tcgatggagc tggttttttt agaatattct tgagcattat tgtgccgatg agcgtgccag
     4561 ttgctactac tcttgcaata ctaaatatta caaatacatg gaatgagttt gctttaatca
     4621 atattttggt atccaagacc gagctcaaat cgcttcccct aggtatctat aaattctctg
     4681 gttctctttc ctctgactat ggcaagcagt tcgcagcgct taccatcgga atggtaccga
     4741 tgctggcgtt ctatataatt tttagaaagc aaattactaa aggtgtggcc gctggtgcca
     4801 taaagggata aaaccctaat caagaataag gagtgagcat gaagcgagag gatgggttcc
     4861 ctcaggattt tgtgtggggt tgttcgacat cgtgctatca aatagaaggt gctgcaaaag
     4921 aagatggaaa agggccttct atatgggata ctttttctca cacgccagat aagatagcag
     4981 gcggccatac gggggacata gcctgcgact cctatcatag atggcgagaa gacattgcgc
     5041 ttttaaggga actcaatgca ggagcttatc gcttttcggt ttcttggacg cgcatccaac
     5101 cagaagggaa gggcgcaaca aaccagagag ggctcgatta ttattcgcgg cttgtcgatg
     5161 agctctacga agcaggtgta gagccttgga tcgagctttt tcattgggat ctgccgcaag
     5221 cactagaaga ggcaggtgga tggcgcaatc gcgatacagc gatgcgtttc caagactatg
     5281 cagccattat gtatgctcat atcggagata gagttcgcca ttggacaagc atgaatgagc
     5341 catggtgtgc tgccttcttg ggccatctca caggcgaaca tgcaccaggt ttgagagatc
     5401 gaagagcagc aaacagggct gtacatcatc ttcttttagc gcacgggctt gcgacaaggg
     5461 cctttaggcg aagcggaatc aaaggcgaat atggcattgt catcaatcct tcgaagccaa
     5521 ggccagcaac tcttcgacct gaggatgtcg aggcttccga gcgagcttcg atcgagcgca
     5581 cctcgctttg gctcgatcct gtatttggca gagattatcc caaagctttt atggacatat
     5641 ttggctccga tatgccaatt gaggcaggtg acatggacat aatagcagag ccactcgatt
     5701 ttattggcgt aaactactat aacgaggaag cggttcgcgc agctcctttt aatgctgaga
     5761 acccctatgg attcgaattt gtccccacct accaaaagaa gaccgaaatg ggatgggaga
     5821 tagagccatc aggcttgcac agaatattga catatatcgc aacgctctgg ccttgcaaaa
     5881 agatgtatgt taccgagaat ggcgcggcat tcctggataa gccagatcca gatggaattg
     5941 tccgagatta tgagcgaata gactacataa ggagccacgc caatgcctgc agagatgcaa
     6001 taaagcaagg tgttccacta aaggggtatt ttctgtggag cttgatcgat aatttcgagt
     6061 ggtcatttgg ttataatcga accttcggca ttgcaaccgt tgattctaat acgctggaaa
     6121 ggcgcccaaa attgagtttt tattattatc gagatctcat tgcgggcttt ggtctttgag
     6181 ggggagaaat gtcggagcta agcagtatca ttcctgctcc tgtcgcctgt gagcctggag
     6241 aaggcagttt ccgctttaat caagaaacat gtattgctac agaacctgga ctagaaaaag
     6301 aagcgggggt acttgcagct tggctttcta gagttccagg gattgaaagg gtaaaaattg
     6361 tctctggaaa ttcagataat cataagcctt tggctatacc aagcgagcct ggaagtaata
     6421 aaactgcgac aatacggcta tctctagagt cagctctctg cttgaaaacc accgaagcct
     6481 atgtactgga aataacgcct tccagtattc aaattacggc tgcagaaggt gctggtatta
     6541 tccgaggtgc cgcaagtcta tggcaacttg ttttagggaa gagctgcgaa gaaggacaaa
     6601 gagtgtcgat tcaggctggc agaattgtgg ataagccaag gtttggttgg cgaggtttga
     6661 tgctcgattg tgcgcggaat ttttttagag tggagtttat cgaaaaactg ctcgatcttg
     6721 ctgctttcca caagctaaat gtgtttcatt ggcatctcac ggacgatcaa gcctggaggc
     6781 tcgatattcc ttcaagacct gagcttgtgg agtatggggc ttttaggaaa gatcagcgct
     6841 atcaaggcga atgcaaaaaa ggcggctatt atagccagtc tgatgtaaaa cgtgtgatag
     6901 attatgcgca ggcaaggcat attttagttg tgccagagat agaattgcca ggtcacagca
     6961 tcgctctttt ttcatctcat ccagaatttt cctgtagggg aagagcggat tccagtgtac
     7021 attttgaacc tgaagaccgt tatgggattt ttgaggatat tctatgtgct ggaaacgatg
     7081 atgtgtttac tttttttgat aatatgcttc aagaagtcta caaaatgttc cccggtccct
     7141 acatacacat gggtggagat gaagcgccga aggatcgatg gctcgaatgc ccggcatgca
     7201 gggagcgaat gcaggcgtta ggctttgcga gcggagatgg taggtatttc cccgaaaagc
     7261 tgcaagcatg gttcatggga agaatatcta atttactcgt taaccacggt aaaagaatga
     7321 tcggctggga cgaggtcgta gatggcggta ttggaaaaga caccatcgta atgtcttgga
     7381 gagggatcga gccaggtatc catgcagctc agtgtggtta tgatgttgtg atgtgccctc
     7441 aaacaagagc ttgctacctt gaccataaac ataatgatga tccagaagag cctgggcatc
     7501 ttggagtctg tacgctaaaa gattcgtatc tattcgaacc agtgtcagag agattgagcg
     7561 atgaggaaaa gcatcacatt ttaggagggc aggccaatct atggactgaa ctaatatatt
     7621 ttggcagaca ggctgaatat atgctgtttc ctcgcctatg cgccctttcg gaagtatttt
     7681 ggagtcctcg ggagatgcgc aatttcgaag attttagtcg acggttaaga ctacacgaac
     7741 atagactaga tacgctagat gtgtcttatt ataaaggaag atttttttag agattgatct
     7801 tctagaatca gtttagctct tagaccaaag aaccccccat cattttgctt ccgatttttc
     7861 cttgcctaaa atatgagctt ttcttattat ttatgtatct tatcctccga ggcattaaaa
     7921 atatgtcagg attttttact atccataatt ctcgcttttt caacaagtat gccgagagaa
     7981 tccagcgctt tgaagtttca atgcgattca aagatttcca ggatttcgaa gatgcacaat
     8041 acttcgagtt cattgaagcc aacaaagcac cgataatttc ctctctcaca atactctgaa
     8101 aacaagccat cagcttttca taggagcaca aaatgcggac tgtccttcgt tatctaaaac
     8161 cttattggct tgatattgta tttatcgttg tactcttatt catccaggcg aatgccgatc
     8221 tatccttgcc ggattaccta tcaaggattg taaatgtggg tattcagcag aatggtattg
     8281 aggctaattt acctgctgta atgcaaaaga gcacttttga agccttgggg agactattcc
     8341 agatggaagc aaagcccgag gattttttag ctctgcaaaa ggtatatgtc gaagtgttgc
     8401 ctgattcccc ccaagctcaa tcgctatcga aaaaatggcc tcttgcaaaa aaagaaccag
     8461 ttatgatgct ttcaagcaag aataaggctg aactcgatgt agctcgagca atttttctct
     8521 cggaatatcc aaaacttgcg gcatttttac agtttagttc aatggcagca ggcaaggtat
     8581 cgccttcgcc gcagatgtcg aagtcgggtc tttctggcct ttcttcaatg acccctcaag
     8641 agctttcggc acagatcgaa gccttagatc ctcttataaa atctcagatt gtagtccttg
     8701 gacttcaagg cgaattcaca gcgcttggag ctgacatcaa tgcaatgcag agctcctata
     8761 ttctgcgagt gggcattcag atgttactaa taacgcttat tgctgttggt tctgctgtgc
     8821 ttgtgggttt cttgggttcg aggttatctg caggcacttc gcgagatctc aggaacgcgg
     8881 tgttttcaaa agtcgaggaa ttttcgcttg cagaattcga ttcattctct acggcatcgc
     8941 ttataacgcg atctactaac gacataaacc atattcagat gctcataatg atgagtacac
     9001 gtatgctctt ctatgcacca attattggga tagggggtgt aattcgagct ttaagcaagg
     9061 cttctggtat gtggtggatc attgcgatgg cggttggtgt ccttcttggc atagttggtc
     9121 ttgtatttgc gctggtggtc cctaaattcc gaatagtcca aaagcttgtc gataagctta
     9181 acctagtagt gcgcgaaaat ctctcaggca tgatggtcat cagggccttc aatatgcaag
     9241 atcatgaagc aaagcgcttt gataaggcta atcgggacct gacaagtatg atgctctatg
     9301 tcactagagc tatggtagtg cttatgcctc tcatgaccat cattatgaat gtggtttcaa
     9361 tatccattat ttgggtgggt tcccatgagg tagctgtagg gagcattcga gtaggtgaca
     9421 tgatggcttt tatgcagtac tcaatgcaga ttttctttgc gttccttatg atgtcggcta
     9481 tgtttatcct tttcccgaga gcgagtgtct ctgcagaacg aattgcagag gttctaaaca
     9541 aggaagttag tattcgtgac ccagagaaac ccattcatct atccaagccg gtcagaggag
     9601 agatcgaatt ccatcatgtc gatttcaagt atccgggggc gcaagagaat gtgctggagg
     9661 acatcaactt tattgcaaaa cctggaacca tgacggctat tatcggtacg acaggatctg
     9721 gcaaatctac tctagtcagc ctaattcctc gcttttatga cgtaacagga ggtcagatac
     9781 ttctagatgg aatagatata aggaatctcc cccttgcaga gcttagatct cagataggtt
     9841 tcgttcctca gaagagcatg cttttttctg gcgacatcga agagaatatc cggtatggga
     9901 aagaagatgc attggaaagc gagatcatga aggctctcga ggtttctcag atcatgaatc
     9961 ttgtggaaga aagcccggaa gggcttaagc ggccaatctc tcaaggtggt atgaatgttt
    10021 ctggagggca gcgtcaaagg cttgcaatag cccgtgctct ggtgagaaaa gctcccatct
    10081 atatcttcga cgatagcttc tcagctatcg actataagac tgatcgagca attcgttcag
    10141 cattaaagaa atatgcggca gacagcgtga tttttcttgt tacgcagcgt gttgcgccta
    10201 ttcgtaatgc agagcaaata atcgttctat atgaaggcca gatagttggc attggtatac
    10261 atgaacagct tatgaagacc tgcgaggtgt atagagacat tgcgctttcc cagctcaagc
    10321 aagaggaact cgtatgaata acaatgaaag gcaagaacaa ctgaataatt catccgcttt
    10381 ccacgaaaca caaatgcaga gaagaccagg agcaaagcca gttcgacctg gccgcagggc
    10441 gctcatggct ggtgaaaagt cgagagattt caaaggcaca atgaagaagc tcattgctta
    10501 tctaggtcct tatcactggc caattctttg tgtgatgatc ctcgcaatag tatcgactat
    10561 tcttgcaata ctgggtccaa aggttcttgg aaatgctaca acagcgctct ttgagggagt
    10621 cgtcaagaaa ttgacaagga ctggggatat cgactttgca aaaatcaaga ccattctttt
    10681 gacggtattg gttctatatg tttcttcctc tatacttcaa tacttacagg gctgggttct
    10741 atctggcatt gcggtcaaga ttacctattc atttcgcaag gacatcttag caaaaataaa
    10801 tcgaatgcct ttcaaggctt ttgacaacac taatcatgga gaaattcttt cacgaatcac
    10861 gaacgatgtt gatgtagtga atcagacact tactcagact atttcccaga tgatccactc
    10921 gatcatgacc atcattggtg ttttcgtgat gatgctcact atcagctggc agatgactat
    10981 agtagcattg gtgacaattc ctctttcgct gatagttgtt cgagtcatag taaagcgctc
    11041 tcaaaagtac ttcaggattc agcaagaata ccttgggcat gtgaatggtc atatcgaaga
    11101 gattttcagt tcccatatag ttgtcaaagc ctatggagga gaagaaagag ctatacagac
    11161 ttttaatgag ctcaattcaa ctctctatca gtcggcttgg aaaagtcact ttctttcgag
    11221 cattatgatg ccgctcatga attttgttgg gaatatcggg tatgtagcgg ttgcagtgag
    11281 cggaggctgg cttgcagcaa agaaaatggt gacagttggg gacattcagg cttttctaca
    11341 gtatgtgcgg cagtttacac agccaatcgt ccaaattgcg aatatctcga atgttttgca
    11401 gcagactgct gcagcagcag aacgcatctt tgattttctg gcttcggagg aagaaaagga
    11461 tgtatcgatt gagatgggac aaaagggagc tcttgtcgaa ataccagaga gtatcaaagg
    11521 caaggttgag ttccgccatg tttgctttgg ctatctgcct gatcagccta taatcaagga
    11581 tttcagcgca acgatcttgc caggccagaa agttgctatt gtaggtccaa caggtgctgg
    11641 caagacaaca ttggtcaaac tcctcatgcg attctatgat ccggatagtg gagagatact
    11701 agtcgacgat ataccgatta ggagtttgcc acgctccttg gtgcgttctt ggtttggtat
    11761 ggtactgcaa gatacttggc ttttttcggg aacggttaaa gaaaacatcg cttatggcaa
    11821 gcgcgatgct accgaggccg aaatcgtcga agctgccaaa gctgcccatg tagatcattt
    11881 tgtacgagct ttaccagaag gctataatct cgagataaat gaagagacga ccaatatttc
    11941 gcaagggcaa atgcagttat tgaccattgc acgtgccatg ctcgcacagc ctcctatgct
    12001 tattctagac gaggcgacga gcaatgtgga cactcacacc gagctcttga ttcagagagc
    12061 tatgggattg ctgatgcagg gcaggacaag ctttgtgatt gcgcaccgac tctctacgat
    12121 tagagatgct gatctcattc ttgttatgga tcacggagat atcgtagaac aaggtacgca
    12181 tgaagagttg ctcaataaag gcggcttcta tgccgagctc tataatagcc agttcgacca
    12241 gaatggcgaa gaccgacttg caattagtgc ttcaagcttc taggagcctt tagtttcttc
    12301 taggagagcc tttagttttc ctaatcgaat tggataatat aatataaata agagaggcgc
    12361 ccttttcggg gcgcctctct ttgcttcgac tgatgatcct ctgactattc tcaatactcc
    12421 attccaccca ttccagcatt tggagccggc attgcaggcg gattcttttc aggcaggtcg
    12481 gttatggcac attctgtcgt caaaagaagc gacgcaaccg aagccgcatt ctgaagcgca
    12541 tagcgcgtga ctttagctgg gtctattatt cccgcctttg tcatatctgt ccactccagc
    12601 tttgctgcgt cgaatccgat gcctttcttt tcctgcttag ccttatctgc gatgatggaa
    12661 ccatcgatac cagcgttttc agcaatctgg cggatgggct cctcgagtgc tcgctttacg
    12721 atcttgaaac caacacgctc atcatcggtc attttagagg tatcagcctt atcgagagca
    12781 atagctgcct gaataagcgc tatgccgcca ccaggcacaa taccttcctc gattccagcg
    12841 cgggttgcag agagagcatc ctcgactcta tgcttcttct ccttcatttc tacctcggtt
    12901 gccgccccaa cattgataac agcaacacca cctgcgagtt ttgcaaggcg ctcctgcaat
    12961 ttttcgcgat cataatcgct agttgtctct tcgatctgca tcttgatctg ggcaattcga
    13021 tcttggatat ctttctgctt gccaccgcca ttgataattg tcgtattatc tttgtctacc
    13081 ttgacagtct tggctgtacc aagctggctt aggcttgtat tctcaagctt taggccaagc
    13141 tcatcggata tgacctcacc gccagtgagt atggctatat cttcgagcat tgccttgcgg
    13201 cgatcaccaa agccaggagc tttgactgca catacattga gtgttccgcg gaggtggtta
    13261 acaatgagag tagcaagggc ttcgccttcc acttcttctg caataataag aagtggctta
    13321 ccagtttgag cgatcttctc gagtattggg aggagatctt tcatgttgga tattttcttg
    13381 tcatggataa gaataagggg gttctcgaag actgtagtca tcgaatcgcg gttggtcacg
    13441 aagtatggtg aagtataacc acgatcgaac tgcatccctt cgacatactc gatggtggtc
    13501 tccatggttt tggattcttc gactgtgatt actccatctt tgccgacttt ttccatagca
    13561 tcggctattt ggtttccaat gtcgatgtca ttgttggcgg agactgaagc gacatgggca
    13621 atctcctctt tttccttgat gtctttggag atctttcgga tttcctggac agaaagggtc
    13681 accgcctgat cgattccgcg cttcaaggcc attggatcga taccagccgc gacagatttt
    13741 aatccttcct tgacaatgga ataggcaagc actgtagcag ttgtcgtacc atcgccagca
    13801 atgtcatttg tcttggttgc gacttctttg agaagctggg cacccatatt ctcgtacgga
    13861 tcttcgagct cgatttcctt ggctacagat acaccatctt tggtcactgt aggagcacca
    13921 aatttcttgt ctagaagcac attcctgccc ttaggaccaa gagtcactct gactgcttca
    13981 gagattgtct ctacaccgac aagaagtttt cggcgcgcat cttcgctgaa caacaattgt
    14041 tttgccatac cttgttctcc tgaaatgaca gtgtttttac cggccgtata cgcatacatc
    14101 gataaaacga tgttgtgaag ccggatattt tccttggatc ggctttgata agcctgtaat
    14161 aagatataaa aaatgataaa aatcggcaag aggatgccaa tacaagtagc ttttcgaata
    14221 ttatcatggt aaaaggcttg aagtccgctt agactatttc caatacatga gatttgagac
    14281 actatactag agaaacaata gagaatctat gatacatttg cagtcgtgaa agaaatatgc
    14341 caaatccttt atgaagatca agatctcctg gccatagaca agcctgcagg catgctcgca
    14401 atacctgatc gttgggacac aaatgttcct gttgcacagc aacttcttca aaatcaattt
    14461 ggcaagctta tgccagttca tcggatagac aaggacacta cgggcgtttt actatatgcg
    14521 cgaaatgagg cagcacatcg tataatcagc aacgattttt cgaatcgcaa agtagaaaag
    14581 atatattttg ccatagtaca aggtgaacca gagtctgata catgggaagt ggaacttccg
    14641 cttcgggctg atggcgatag acttcatcgc acgattatag atgcatcaaa aggcaaagaa
    14701 gccaagactc gctttgaagt aatagagaga tttcggggct tttctcttgt acttgctatt
    14761 cccgaaaccg gcagaacgca tcagatacgt gtacatcttg cggcttctgg gcttgctatt
    14821 gttgcggacc ctttatatgg caatggagct cctcttttgc tttctatgct aaaagctggc
    14881 tggaaaggag atgcatacga agagaggcct cttatcgacc gaacggcttt gcattctgca
    14941 aaagtgagct ttgtgcaccc aaaaaccaag gaaggattaa taatagaggc gccgttcccg
    15001 cgcgatttca aagcaactct cgctcagcta cgtaaagtcc gtacaagatg ggcatagcaa
    15061 ttagcaaaaa gtcatttcca tgttctatgg aatctctcga gagcatcaat gactttttcg
    15121 atctgttctg ttggcggaag aatagtggtg cggaaatgag cagtacctgg cttctgtcca
    15181 aagccactgc cagctactac acagactcca gtctgctcga gaagcgccat acagtaatca
    15241 tcatcgcttt tcccaggcgg caaagtaata gatggaaagg catacatggc tccatatatt
    15301 ggctggcagt ggtagccaga aatgctatta agtccttctt cgagcatctt tgcacgcttt
    15361 gccaattcgg ttaggattgc gcttcgctca tcggcaaatt gctggaaact cggatcctga
    15421 ggttttgggg ggcttaccat taggtaagca aggatctgtc ctggcaaatt ggcgcagagc
    15481 gaaatcgatt gaagcttagt gatttgatca ataacttctg gtggcacatt tcgcacttcc
    15541 atatacccgc cgcgatgtcc gcactcgcca aagaatccct ttgataccga atggaagctg
    15601 aaaagactta cttctttctc cttcatagat gtcatgacat gcgcaaagga aataaatctg
    15661 gctcctggct tatatatatt ttcttggtat acctcgtcag cgagaatggt gagattatgc
    15721 ctctttgcaa aatgtatcac catgcggata ttattttcgg tgagtactcc tccggtagga
    15781 ttgccaggat ttattaccac tattgcacga gcgtgaatgc ctttggccag agcttgttga
    15841 atagcatctt ccagcatctg ctcatcgaga ctccagcctg attcttcatc gagatagtag
    15901 cctatctgct gtccaccata gagagtgatt gtcgcagaat agagaggata ctgcggtata
    15961 ggaatgagaa tgccatcatt cttttttgca ataagaagag taagcgctgc ttggacgcct
    16021 ttggaggcgc catcagtaag atagatattc tcgaaatctg cattctgttc cactccgtta
    16081 aagctatcgc gcgatattat aaagtctgca atagccttgc gcacaaatct aagtccttta
    16141 gactcagaat atgctcctag tccatgcttc gattgctcga ggatatagtg tgctttttct
    16201 ttggcatctt ccataagatc tatatttggg ctctttagca gttcaggctg ttcacaggcc
    16261 gctagcgtgt ttcgaacaaa ggtaagcggc ttttgcttaa gggcttgggg gtttccaata
    16321 ttgcaataga ttatttcctt tcctgtattc tcaagttgtt gtgcacgagc tacaattggc
    16381 cctcgtaccg catagtaagt attgattact gcatcgctga gatccgattt ttgaagaggc
    16441 atgaggactc ctcctttgaa attgattggg aattatcata tattagcatc tataaagcag
    16501 cggagtcgag gccgatgtca aatctggatg aattgtgtaa aatataacga tgaatataag
    16561 catgacagaa gtaaaaagaa tctctgaggc ttcccgcgta tttctcattg gcgtagcatc
    16621 gggcgagatg cctaagccag aagcgaaaga actgctcgat gagctgcata ggcttaccga
    16681 cacgcttggt caggaagtgg taggagagat gttgatactc ctccgtgaat atacatcatc
    16741 tctgctggta ggaactggaa aagcgcagga gatagtcgat gctgccaata aagcagatgc
    16801 tgatactata atcttcgatc atgtgctgag cccagtgcag cagcgaaact gggaaaagct
    16861 atcttccaag aaagtttatg atagagcaga acttataatc aaaatattcg catcgagggc
    16921 ttttactaag gaggccagtt tacaggtcga acttgctcaa ctcgaatata cattgccgcg
    16981 tcttgcccac tcctacaatg acctgatgca gcagcgcgga ggacgctatg gcacaaaggg
    17041 ttccggcgag caaaagctag agctcgatag aagagagatt gaacgaagaa ttcatgaaat
    17101 aaaaggcgaa ctggagacgg ttcgcaaaga aagagccgtc cagcgcaagc gcagagagag
    17161 gagcggtgtc ccaagggctg ccattgtagg ttatacaaat acagggaaat caagccttct
    17221 aaatgcgctc acttcggcaa gcgtgcctgc agaagacaaa ctatttgcta ctctagatcc
    17281 aactactaga agaatacaat tcgtcagagg gcagaccttg ctcattacgg ataccgttgg
    17341 ctttatccgt aatttgccac ctgggctggt ggaggcattt cacagcacct tggaagaagc
    17401 tagccttgca gatgttctca ttcatgtagc cgatgcctcg gacaaaaaca tcgatataca
    17461 tatcgaaacc acgatgaagg ttctctccga tattggagcg ggcgaaatac catatatcct
    17521 cgtgttgaat aagatcgatc ttgtagatct ggacacggtg caggctttcc tttcgcgcta
    17581 ccctggaagc gttgcagttt ctgcaaaaac aggagaaggg cttggagagc ttgtaaacga
    17641 aatccaagaa gccttgacaa agcaaatgga gccattgata cttcgcatcc cgcataatga
    17701 ataccatatt gtaagtctca ttctgcgcga agctacagtg ctcgatgaac actatgatga
    17761 agaatttacc tggattagtt gccgcattcc gcgtaaactc ataaatgctg ttcaggaata
    17821 cgcctataat ggggctttcc ctctagtgga ttccaataag ccataatcct aaactaattt
    17881 gaaaggatgt ttttataatg aaggaaatcg tttctgtggc agtagttgga gctggtgcga
    17941 ttggcgcttc tatagctgtg cgcctcaagg atgcgggcaa acaggtgata atatctgcca
    18001 atggagagag aaaagatcgg tacctaaagc aaggatttgt cgtaaatggc caacaacatt
    18061 tcctgcctgt aagagaccag caagaggcag ctccagtaga ccttattatt gttgcagtga
    18121 agaattacag tctcgaagag gccatagagg agatgaggcc gtatattaac gcgaatacta
    18181 ttattctatc gctcttaaat ggaattgatg ctgtgcccag acttcggaaa gaatttggcg
    18241 aagatagggt accatatgca atgatcctcg gcatcgatgc acttcgcgaa gagaataggg
    18301 ttcaatatct agccaagggc aagatatttt gtggcttcga gaaagagaag gctcaaaaga
    18361 atgctcctaa gctagctctc ctcgaagagt tcttcagtac gagcgatatc actttcgtgg
    18421 tgccagaaga tatagtaaag gagatatggt ttaaattcat gatcaatgtt ggccttaacc
    18481 aatggtctgc cctgataaga tctccatatc gcctttttca aaactctctc catggccagg
    18541 agcttcttag gcagacaatg atggaagtaa ttgcattatc tagtcacttc gacggtaatc
    18601 tcgataaaac agatatcgat cgtgccatcg ctgtcttaca gacacttgct cctgagggca
    18661 agacatcaat gcttcaagat gtagaagcaa gaaggcagac cgaggtcgat gcttttgcgg
    18721 gagcaatgat gcgtcttgcc aaggctattg gtctcgatgt acctataaat gcgatcttat
    18781 acgaagctat tagggctatc gaagattcat acaagtcgca gtagagatat ctctccagag
    18841 acgaaagttc ttttctcgcc attattgctc agaatgaggc ttccattttc atcgatacct
    18901 tcacagatgc cttctgcttc ctgtgcaagc tgggggtgtc caataagaaa gtgcatagga
    18961 gtgcctagcc cccaaagtct atactcgtat tccgaatgcc aattaggatc gtggagtgct
    19021 tgaaggacat gctcggttat ggaacaagcg gcgctgtcga gattggaaaa ggcttgcgga
    19081 ataaacccta atgcttcctc taggctgaaa ggtgagagat cagtttgcag accatcgtag
    19141 ctcgatttca gaagattgag gccgatacca atcaagacat tatctatgat ctgttcgcag
    19201 aggattccgc aaagctttcc aaagtgacaa gaattagaag gaaggaggac gacaatatca
    19261 ttaggccatt tcaagaaaaa atatggctct cgtttgcgtt caaattggtc atgattggcc
    19321 gtaagccttg aggacacccg agtttctaaa gcttgatttt ccaaaattgt ctccaatgcc
    19381 cgcactacac caagccccgc acggagaggt attgcaacaa tgcccttata attttttggt
    19441 agaattatag tcataagaag gcagtgtttt ggcgtgtcgg tccattttcg gccttcgatc
    19501 cgcccgcggc ctgcggtctg agtatcagcc cgcactattg taatatcatt atctagcccg
    19561 tttcgaataa ttcggcgtgc ttcatccata gtcgagtcaa ggctcgcata ggagagaatc
    19621 ttgattgagg atggcttggt cgagaagaat gagcgcaaat tatttttcat atattcaagg
    19681 ctatcgaaat tgccatctag cgacaagagc catttagata tctaagtatc gaacggcaaa
    19741 aatctttgtg tctttgaaat cttaggggag tttgaaggtt catatcgctg tgatatcatc
    19801 gctttaggat tattcgctgt tatccaaaaa gggatgaaat gaaacatgat gcatggcgtg
    19861 tcttgccaat tgtagtattc gccgtcgctc ttttaggagc acttgctttc aacatcgctc
    19921 tagttagcag aagccgcaat gccctcgcat caactatttt gcgtaatcca tccactattc
    19981 atagcgctcg ttttcatatt atcgctatcc ttccagatac agttgatcca ttctttacac
    20041 acttgattga gggcctgtcc gaggaagcag aaaaacaaga tgctgcacta caaatatttt
    20101 atttttcgcc tactggcgcc atcgatagtg gcagcatttc tggagaagta ttgcgctggt
    20161 tcgagattgc attgcgcagc aagaccgatg gtataatcct ttttcaaacc gctggcctcg
    20221 atgtgaagaa gcttgcagaa gcagccgaaa atgcgcagat accatttgtc ccaatggcaa
    20281 tggatgtttc ccagcaatgg ataaatgctg gaataacggg tgattctgct agtcagggga
    20341 gggaggcgag cacccttgct ttgagtttgc taggtgccgc agcccgtatt ggtattattc
    20401 tctcttcaga cacatcttta ggatatgctt tctatgagga accattctac agaggagtag
    20461 aagagacact caaaagcaag caaggagccg caattgtcgc tgcagagcgc gaagaagaga
    20521 gtattctcgg tggagaagat gcttgtgcgc gcatgctagc tgaacaccca aatattaatg
    20581 cgatcctatg catagacgct aaggctacaa ttggcgctgc tcaggttgtc atagatcgtg
    20641 gtatggtggg acagattgtc atcataggag ctgatgaaaa ctcagaggtc aatagactaa
    20701 tcgaaaaagg cgtggtccag gcaagcatcg ttagagatgc cgtggctatg ggtagagctg
    20761 gcgtggctct cgcgattggc cagcggatcg gcattcgggc tccggagaga atctcagtag
    20821 gatacagagt gatccctgcg agaggaggag gtttatgatc gactttgcat tgatctctcg
    20881 acctctaaaa gccagacggc acaaggagaa aggccttcgt gatcgcataa tcgctaattc
    20941 cgcagtaata cttcttgttt tagtcatagc gacatcctac acagcatcca cttcttttaa
    21001 tttggcgcgc agtgtagagc ttctattcca taattccgta tcgatggaag aactcagaac
    21061 agtcctgaga gaaacacagg ataatcttac aggctaccta agtgctaaga attcggaatc
    21121 gctaagagaa ttcatactca attcaactct cctttccaat atggcacata aactcaataa
    21181 agaaaacaaa aatgacgatg tctttttgct cgaaaaggat ctggcttgcc ttgtagagag
    21241 ctatcttgcg agtgcagagg gggctgtcca agctaaacgt ggccgcgatg tcacaaagta
    21301 tgtaagcctc tatgaggaag caaggcgtgg agcttcttac atagaatttt tgagtacgag
    21361 aatggatact atctatttgg gccagtcatt aactggattt tcttcgtatc gagcgaatat
    21421 cagtcttgca atcatgtcca atggagtgct attgcttgca gcctttttac tttcgatagc
    21481 cctaattgga aggtattctt atacaattac tgagccactg gcgcggcttt ccgtggctgc
    21541 agatgcagtg ggcagaggag agtatgacca tcctctacca ccatatgaac gagaggatga
    21601 gataagaacg cttcatgatg catttgtcaa tatgaaggat agtgtccaaa aggcttttgt
    21661 cgaattgacg agaaaggcag agctggagca ttcgctgatg gaagagcgca tgcgcgttct
    21721 tacatatcag caccggctaa aggatgcgga acttctggcg cttcaaacgc agatcaatcc
    21781 acactttcta tataatacat tggcggctgg ttggcagctt gccctctctg aaggcaatga
    21841 gaaaaccgcg gaattccttg aaaagcttgc agcattcata cgttactcgt tgaagccttc
    21901 tactaggttt gttcttgtct cggaagaaat agaatgcgtt cgccaatata tttggctttt
    21961 gaggctgaga tttggcgagc gctttgaatt ctgcctggac atacaggatg aggccctagg
    22021 ctatgaaacg ccggcgcttg tgcttcaacc ccttgtagaa aatgcaattg tgcacggctt
    22081 aaaggatgtc gaagaaggag gcaaagtaat aatatcagcc aaactggaag gagaaacaat
    22141 ccgtttctcg gttagtgatt caggttgtgg aatgagcagt gaatacatca cacttgctct
    22201 tgcggcagcc agaccagatg atacaagacc tcaaaatggt attggcttac acaatgtagt
    22261 taggagagtt gctctcgcta caggaggtct gggcagggtt gaaatcgaga gcaaacttgg
    22321 ccatggtact tgtgttacga tcctcttgcc gacaggaaaa acatcatgaa gagagtgctc
    22381 atagccgacg acgagattcc gatagtaaat ggacttcagc ttcttttcaa gcgctatttc
    22441 cagagtggct atctagttgt tggaaccgct cagtctggcc gcgaggttat cgagaaaagc
    22501 aaaactcttg caccagacat tgtacttatg gatgttcaaa tgcctggcat caatggacta
    22561 gatgcaatac gagaactctc ccagcagggc ggaaccaagg catttatcct cgtgactgca
    22621 tacgagcgat tcgatattgc tcgtgaagct ctttctatgg gtgtctgcga ttatctttta
    22681 aaaccagttt ccaaggaaag gctcgagata gctttgcaag cagcatcttc ttatctcgat
    22741 cgtcttcata tactcgaaga aaaagagctc gaattcaaag agcaccaaca aaagctgcta
    22801 ccctttgtca aaaccgcttt ctttgtatat ctacgccagc agcgtggagc taaagacaga
    22861 gagccaagag agactaaacg cacaaaagca ttgagagacg agctcaatct gtacaaggaa
    22921 gtgctacaca tttcaggaga agctggtgtt atgggtattg cgagtttttc gccagcagac
    22981 ggcttggcaa actcgctcta tgagtatttt tgttctgctt tgcaatataa aaccatagct
    23041 ctagcaggac cactagaaga caatcgttat tgtgcatggt tcttgccgtt gaaatccaaa
    23101 atggatcaag gtactgccgc cgaagctgag gtcacagcct tccttgaatt gctcagttca
    23161 tccttttcat cacagcttgc aacaggggaa ttgaggtttg cttgtggtca gccagaattg
    23221 cttgagaata ttggttgttc ttgggataac gccgtgcgta gctttgggct tagttccgaa
    23281 tacataatta gttccagaca tcgcttcgaa gaagacataa tagaggatcg agaagaacca
    23341 gcttcggcat tcgatgccca gttctatgat gctattatgg aaggtcaata tgccatggcc
    23401 ggacagctcc ttgaaaagct aattctaacc gtggaagaag cagaagctaa accgcgcgat
    23461 ctatatttta ggataattgc agcgctttcg ttctgggctt ctaagcttgc aggaggagga
    23521 gtgctctccg ataatgtcta tagagatttt atggatttta gcgatatcga aaaattatgg
    23581 gaggagcgct tttggcagct attcgcatca gcagtaagaa gcagattcca gagtctgcag
    23641 aagcacgcag ctgagatagg gactcattcg ccctttgtgg tcaaggcttt gcaatatata
    23701 gagaacaatt atcaggaacc gataacgctc gaaagtgctg ccgcagcaat aggtatttct
    23761 gcaggacatc ttacacggct tatgtcagac gaactaaaga gaggcttcgc acggaccttg
    23821 atagggtaca gaattcagaa agccaaagag ttgcttaaga tgccaaacat ttcaatccgg
    23881 gaagtgagcc aacgctgtgg atattccgac gctaattatt ttgcgcgtct gtttcgtcgc
    23941 cttaccggac ttacgccgcg cgaatatgcc gcgcgtatgg caaagggaga aaatagcgat
    24001 gcctaaaaca atgtataagg cccatagtca tgtttttttt atgatgacta ttatcttagc
    24061 aactataact ctcagttatt tttcatgcag taaatcggaa aagacagtcg aaaccaagtc
    24121 gcctttgatt ggattttcgc tggattctct ggtagtagaa agatggagac gagatgtaga
    24181 tagctttact aagacagcac acgatctcgg ggcagaggtc gtgctacgag tggcgaacca
    24241 gaacgctgag actcaaatag cgcaagttcg agagctctta ggtcaaggca tagatgttct
    24301 ggttataatt ccaaatgatg cggaaaggct ttcggaagta tgccgtgaag caaaaagaaa
    24361 aggcataaaa gtgttgagtt acgatcgact tgtccacagg gccaatgccg atctgtatat
    24421 ttctttcgac aacgaaaaag tgggaagtct gcaagctcaa gcgcttgtag aagctgttcc
    24481 acaggggaat tatgtaatca ttaatggagc taccacagat aacaatgctt tcatgatcaa
    24541 taaaggtttt cattcggtgc tcgatccatt cattgaatct ggcaagattc atcttcttga
    24601 agagatttgg ccaagcgatt ggatgagcga cgaggtaaga acacgatttg aagccctagc
    24661 aaaaccaggt cagaagatcg atgcagtgct ttgcggtaat gacatgctcg cagagacagt
    24721 aatatcagtt ctctcggaga atagaatggt tggaaagacg aaggttactg ggcaagatgc
    24781 cgaactttcg gcctgtcaac gcattgccga aggaagccaa tttgcaactg tctataagcc
    24841 tattgatagc ttagcgctca aggcagcggg ttttgcggtg atgatggcta atggcgaaaa
    24901 aatacgatat gagaataaga tagaagatgg tagctacaag gttccataca ttgcactcga
    24961 gccagttctc gtgacagcaa agactttgga gtcaacagta atagaggatg gttttcatag
    25021 ccgcgacgat gtatatcgca atgtaaaaca ctgaataagt aagcctgtct tatcccaaga
    25081 ttttcattct ggacattttt gaagctgttt tgtcttaaaa gtcctaatac tacattgagc
    25141 aagctatata tgcgcaaata cattagattt gtcacaaaat tcctaatgcg tgattaaaaa
    25201 atctagcgga aaatgatagt tggcgcttac ctaattttac caaaagcgcc aaactagtcg
    25261 ctttgcgatt agagcctata gttttctaag gaggatattt gatgaaacga acattactca
    25321 ttgcaatgtt gtttacagta gttgcaggat ttgtatttgc tgagacacaa gtgggcatcg
    25381 tgctccccac taaggatgag cctcgctgga ttcaagacca gactcaattt ctagatgctc
    25441 taaagactgc tggattcagc gcaaaggttc tttttagcca gggagattct gcaaaggaaa
    25501 aagccaatgt cgaagcccta gtaagcgaag gcattaaagt tctcattatt tgtccccatg
    25561 atggaaccgc cgctgcgtcc gcagccgatc ttgcccatag cgcaggtgtc aaggttatat
    25621 cttatgaccg actgattcgc gacacaacta gcgtcgatta ctatgtaacc ttcgacagct
    25681 ttgaggttgg aaaagcttgg ggcgattatc tcaactccaa ggttcccgct ggcacaaaag
    25741 gcaacaacct atatctgtat gcaggagcag caagtgacaa taactctttc atattctttg
    25801 aaggagcgtg gagcgccctt cagcccaaaa tagctgatgg cacatatatt gtacggaatt
    25861 cggacaaagc cgtagctctt gcaaagaaag ccaagctcac tcgcgatgaa gaagctcaga
    25921 tcattggcca agtcacgacc aactggaact tcagcgatgc caagaacaag gccgaagcca
    25981 acttaacagc agctcctaag gaagccaaag ggaccgtgta tatctgcgct cccaacgatg
    26041 gcaccgctcg tgcaattgcc gatgcttttg ctgccgataa agatgtcaag acctattaca
    26101 taactggcca ggatgctgag atagcttcca ttcagtatat catcgatggc aagcaatcca
    26161 tgactgtcct caaggatgtg cgtacgctcg taaaggatgc aataaacgca gctaccgcct
    26221 acatgaaagg acagacacct cccgttacgt cctattacaa caatggcaag aaagatgtac
    26281 ctgctaagcc aacagccatt gtcactgtca ccaaagagaa cgtaaagaaa gagatcatag
    26341 attccggcta ttggcctgct gacaagttca ccggcctcaa gtgatcattg gggtgtgtaa
    26401 acatgcccat ttgagtacga tcagagaaga gccgctcatt acgggcggct ctctcttgta
    26461 ataagatcaa tccaggcggg tacaaattat gaatgacaat attcttgaaa tgcggcatat
    26521 cacaaagagc ttcccaggtg ttcgagcatt gaatgatgtc aactttgcag tgcgccgcgg
    26581 agagatccat tgtttggtag gggaaaatgg ggctggaaaa tcgacactta tgaaggtctt
    26641 gagcggcgta tatccgcaca atgaattcga aggtgagatt atcctcaatg gaaagaaagt
    26701 agcgttcgat ggtattcacg atagcgagga agcgggcgta ggcattatct atcaggagct
    26761 agctctagtg cccgagatga ctgtctatga gaacatcatg ttggggcatg aaatccgacg
    26821 tggtataacc gtcgatgtac atgagatgat aaaaaaagca gaagagcttc ttaagagggt
    26881 cagactttct atcaatccga cggccaagat caaagaccta ggtattggca agaaacagct
    26941 tgtcgagatt gcaaaagctc tgagtcgaaa tgtatcgctt ctcatactcg acgaaccaac
    27001 cgcgacatta aatgaggatg actgcgaaaa cctgctagat ataataagaa tgcttaaaat
    27061 gcaaggtgta acttccatca tgatatccca taaactaaga gaggtgctct ccattgccga
    27121 aacgataacg gtgctccgtg atggtcagac tatatgcacc cttgatcgcc gcaacgatga
    27181 ggtcaatgag caagttctca taaagcatat ggttggacgt tcaatagaga acatctatcc
    27241 gccccgcata tccaaacctg aagaaacaat ttctctcgaa gtaaagaatt ggaatgcttt
    27301 cgatccaaaa cttggaagaa aagttctgaa taatgtcaat ttctatgttc gcaaagggga
    27361 agttgtggga ctctccggtc ttgtaggttc gggaagaacc gaattagcga ggagcctctt
    27421 tggaaatccg gacaaatatg agatttctgg cgacctcatt ctaaagggaa agaagcagcg
    27481 cttctctcat cccagccaag caataaaggc aggactagcc tatgcaacag aagaccgaaa
    27541 agccaatggc cttattctta ttaagtcaat ccaggaaaat atctctcttg ctaatctgca
    27601 aggggtatca aaaaacggcc ttataaacga agaactcgat aaacagaccg ctctcggctt
    27661 tgtggattcc ctatcgataa aaacgccggg aattcaccag cttgtgatga acctctctgg
    27721 cggaaatcag cagaaagtct cagttgcgaa atggctattt gttgaacccg atgtgcttat
    27781 attcgatgaa ccaactagag gtatcgatgt tggcgcaaaa tatgaaatct ataacattat
    27841 aaatgatttg gtagcaaaag gcatgagtat tttaatgata tcgtccgaat tacctgaaat
    27901 actgggcatg agcgatcgta tctatgtcgt agcgaatggg acgattactg
//
LOCUS       DAOSOB010000021        27047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1785_length_27047_cov_79.525267, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000021 DAOSOB010000000
VERSION     DAOSOB010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 27047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27047
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1785_length_27047_cov_79.525267"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..1298
                     /locus_tag="PLS88_03650"
     CDS             252..1298
                     /locus_tag="PLS88_03650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU06476.1"
                     /translation="MPEGLRRIKEREKRWYSRLAEGLASFFIALQVALRRLLAWGRTR
                     LVFAMIPQSEKPSRKFEIRRFSLLAIVLVIVLSVSAAIISIGAYSAKSVRIISLEAQL
                     SDAYKSIDRMRESNENLLSETAKFEEKLEQVIEIAQSKGKPTVKQSTSEEILKVAGLD
                     DPTLGQLSNDPVSRDLLKLNSIADALDKSLGALDDIAKMIASQKDIMTEVPNIWPIKG
                     NLGHITMYFGQNENPFSTGQWYLHTGIDISTNRIGDAVLATADGKVIDIHYDSSLGNS
                     ITIQHSHGFTTRYGHLRSFAVKKGQRVSQGDVIGYLGNTGKTTGPHLHYEVYLGTSLI
                     DPLRFLSIRKEYHE"
     gene            1291..1629
                     /locus_tag="PLS88_03655"
     CDS             1291..1629
                     /locus_tag="PLS88_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU06477.1"
                     /translation="MSRRDFSDCMIGEGSKVDGTIDAPGMVRIDGEFSGDIISASSVI
                     ISKNAVVHAQINARQLVVAGIFSGSAIIEGGVRYAETAIIEADCTGRFLVVEAGAMVN
                     GQFARIDAED"
     gene            1703..2923
                     /gene="ricT"
                     /locus_tag="PLS88_03660"
     CDS             1703..2923
                     /gene="ricT"
                     /locus_tag="PLS88_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOU06478.1"
                     /translation="MSIEYNDHELPEDILDILGEEEFESPKALKPKPKDTENFPQPIY
                     RLKTLHSNETFHAYYVSLENNEHFMTLCELEAALAGPRQANNGTSPSEEAWNCKHGSA
                     SAVTEALQAEAIQEEALQAEAIQEKSIQEMSIQEEGIQAEVIQADAIHAKSELIPNRA
                     LVIAPTKYGKDLVMVLGVVHDLSMVNADELVAIERPATVDDYRRYLENLEHEEEAFSK
                     CRECIEAHNLPMKLISAHCLLEDSKILFFFTAESRVDFRDLVKDLVGIFHARIELRQI
                     GVRDEARVTGGAGVCGRILCCHGLSDKLNPVSIKMAKDQNLSLNSVKISGPCGRLLCC
                     LSYEHQFYRDARRELPNEGVRFTYDGILFKVIEVNALLNKIRISGEDGRILDMDAARL
                     KYLDGHWTVIEEDN"
     gene            complement(2965..4734)
                     /gene="aspS"
                     /locus_tag="PLS88_03665"
     CDS             complement(2965..4734)
                     /gene="aspS"
                     /locus_tag="PLS88_03665"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOU06479.1"
                     /translation="MQFEQRTVTCGELRAAHIGKTVVLNGWVHRKRDHGGITFINIRD
                     RYGVTQVVVDVDSAQELLDIANELKMEYCVSIRGMVRPRPDTMVNPSMPTGEIEVKAE
                     NIQILSRCSSLPFMIDEQTDAKEDMRLTYRYLDLRSFSMQRKIRLRHEAAFAVREYLV
                     GKGFYEIETPTFIRSTPEGARDYLVPSRLYPGKFYALPQSPQLYKQLLMISGFDKYFQ
                     LARCYRDEDSRGDRQPEFTQIDIEMSFVTRDDVLSLVEGMMGHLFKKTLGVELPKQFV
                     RLSYEDAMDRFGTDKPDLRFGMELVDFAPFVDDFCSFQAFHNALAKGGAVKALVVPGK
                     ADYSRRQIEELEEKTKVYKAHGLAWMKVSESATLEGGVSKFFADHALVAQGLSAKPGD
                     LILLVADEKKNIACSALGAVRSQLGKDLDLCDPEVFAFAWIVDFPLFEWNEEENRWQA
                     AHHMFTMPQAQYHETLEADPGSVKGDLYDLVLNGYEVASGSIRIHDPELQKRIFRIVG
                     LSDEEAQRKFGFLLEAFKYGPPPHGGIAPGFDRLVMVMAGETSIKEVIAFPKNSFAMS
                     PMDQCPNEVDERQLKELHIRIVD"
     gene            4911..5903
                     /locus_tag="PLS88_03670"
     CDS             4911..5903
                     /locus_tag="PLS88_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HOU06480.1"
                     /translation="MKRFYVFGLILLILVPAGFAAPKSKVKIALIVESTVDDKGWCQS
                     MHDAIAAVQKKYGSSLVEYNYSEKMKPVDAGSAARQYVAKGFDIIIGHGAQYNNVILE
                     MAEEFPKTTFAFGTSGEIGPKNVFTYMPQSEETGYVNGVIAGLVTKTNIIGIVGPVDG
                     GDAARYNRGFILGVRSVNPKAQVQVAYTGSFGDFVKAAELAHTQIKAGADVLTGSAQQ
                     ALGALRAVAEYKDKAVWWLGQDTMQLTIPESYKVIAAGGYAYEPVVELFIDKRQAGIL
                     GGECIPLSFTNGGFVYQFNDKVGPILSPEVRKAAEKAKADITSGKLIIPWKDVK"
     gene            5966..7516
                     /locus_tag="PLS88_03675"
     CDS             5966..7516
                     /locus_tag="PLS88_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06481.1"
                     /translation="MTKTKDKIYSLEAKGITKRFPGVLACDSIDLSIGENEILAIVGE
                     NGAGKTTLMNILMGLYQPDEGEIRINGRTVRFRTPNDAFAAGIGMVHQNFMLVPNMTV
                     AENVALGSRDFHKFLKLDLAGAKKRIIEVSERFGLPVQPDTYVWQLSVGEQQRVELIK
                     TLCFGARFLILDEPTSALTPQETEELIVLLKRMAQEMSIIFISHKLAEVTALSDKVVI
                     LRHGRVVFSGTTSEQTPSGLAALMTGHEVVLPKNESSEECGDVCFDLENVYAKSDRGY
                     MALDGVSFSIRQGEILGLAGVSGNGQKELAEAIAGLRKIESGRISLFGKDLTNLRPRE
                     RIDQGLGYIPEDRNSEGIVPSFSVKENFILKDFASPDFANGPFLKLKNGEQKAVELKE
                     KFDIRCPSVTTPAGALSGGNIQKVILARELARNPKVLIAVYPTRGLDMGAEEYIHKKL
                     LEMRSQGTAILLISEELEEIMNLSDRIAVIYKGKILRILTSREASMRSIGLLMAGITE
                     PMAMEAGQ"
     gene            7513..8634
                     /locus_tag="PLS88_03680"
     CDS             7513..8634
                     /locus_tag="PLS88_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06482.1"
                     /translation="MKSTIRIVYIVLISLAAAVVAMAIAAIVLGILGASVTQTFKIIL
                     TEPLKDIVGITEILVRAIPLTLVSLGIAISFRSGILNIGAEGQIQMGVIAGAAVAIAL
                     PNMPKFLLVPLALLAGTIGGALWSFVPGYLRAVLGVNEILSTVMMNYIAVQFYGFLLR
                     GPMIDPAELQVGSGTPQSVRLPRSAWIDRIIPGTRLHWGIAIALVLALVVYILLWRTT
                     WGFRMRAAGASPKSARYAGINVERCLLMAMVLAGAFAGLAGAVEVTGVHRRAIEGISS
                     NYGFSGVVVALFGGLHPAGIIPSAVFFGLLLVGADMTQRMISIPANMVLVLQGAVILS
                     IISAKMLINDPYLYDRFARRFTSGFFGSRRLKKPKEGRA"
     gene            8631..9581
                     /locus_tag="PLS88_03685"
     CDS             8631..9581
                     /locus_tag="PLS88_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06483.1"
                     /translation="MSFFMFVNNVLSIGIPFSVALLLAALGEMFNQRAGVFNLGCEGI
                     MSMGAFIGFLPAYYLQETALAPYANMIGIMGAAGIGVLLGILYALVTVTFKAPQGIAG
                     IGLQMLGWGIAGTLFRFFIGGVTGVPGLPNRPIPLLSKIPFLGPIIFSMNGLAYFAFL
                     FVPFSYYLLFKTPWGLKVRAVGTYPRAADTMGIPVSKTRYQALALGGLLAGLAGAYLS
                     LGQAKMFADDIVAGRGFIAVALVYFGQWNPLGILGGALLFSIAQSLQLAIQVLGINFP
                     YEFAVMLPYVLVIVVLAITRGSAIKGPTELGKAYDREKRI"
     gene            9578..10963
                     /locus_tag="PLS88_03690"
     CDS             9578..10963
                     /locus_tag="PLS88_03690"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOU06484.1"
                     /translation="MTEKSLPDFRIETLGNCPVNSPLGLASAPGEGRPVFVPEDRFIY
                     YNIERDIRKKTGSSASKGKKEQGCNSSVEKVDQLLEVAGPRQQIYFSPSHVHAGIVTC
                     GGLCPGLNDVIRSVVRSLWYLYGVRRISGIRYGYKGFLPEYGFGVVPLDPDIVDDIHK
                     IGGTMLGTSRGGGERTSDIVDTIEQLNLNMLFAIGGDGTQKGALAIAEEMERRGLRIA
                     VVGIPKTIDNDIEFVEKTFGFDTAVVKASEAVSAAHMEAHSQINGIGLVKLMGRDSGF
                     IAVQTVLAVHEANFVLIPEVPFDLEGPNGFLSALEDRLERRGHAVIVVAEGAGQHFFD
                     QPVTYDASGNKKLGDIGLYLRDQIQAHFSEKNIEINLKYIDPSYMIRSAIAEPIDSMY
                     CERLGNNAVHAAMAGKTKMLIGLVNNEFVHIPIRAATSRRKLVNPEGSLWRDAIEATQ
                     QPFSMLKKDQK"
     gene            11040..11447
                     /locus_tag="PLS88_03695"
     CDS             11040..11447
                     /locus_tag="PLS88_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOU06485.1"
                     /translation="MDRIFAISDTVNTAVHALALAAASGGSISASKAAKELDVSPSYF
                     AKILQKLASYGLLVPSRGKRGGYSLSKPAEAISCLDVLIALEGEFPKRECLFQHSICR
                     KKTCALRILCEVTSTNLRHALENTTIAAVARSF"
     gene            11481..13136
                     /gene="hcp"
                     /locus_tag="PLS88_03700"
                     /gene_synonym="priS"
     CDS             11481..13136
                     /gene="hcp"
                     /locus_tag="PLS88_03700"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOU06486.1"
                     /translation="MFCNQCQETLRNSGCNIAKGMCGKTAETAHLQDLLLYVCKGIGY
                     WADNARAVGYFPEEDAFFVTRMLFATITNANFAPSDFEHWIMEALKRRDRIRQEFSER
                     GGKIDAIVPDAATWNAVDLVAIRMAAAQDKGGLLEITDEDVRALKALVLYGLKGMAAY
                     LEHAYVLGVSSKEVFEFMFSALAKLADDTIDKEALIQLVLDTGKYGVEAMALLDKANT
                     GSYGSPRITKVSTGVRNRPGILISGHDLRDLHDLLEQTKGTGIDVYTHGEMLPAHYYP
                     FFEKYDNLYGNYGNAWWKQDSEIEKFHGPVLFTSNCLVPPKESYKNRIFTTGVVGFEG
                     VTHIPDREPGTMKDFSAIIEMAKTCSPPEALEDGEIVGGFAHDQVFALADKVVEAVKN
                     GAIKRFIVMAGCDGRQPKRSYYTEVAQKLPKDTVILTAGCAKYRYNKLNLGDISGIPR
                     VLDAGQCNDSYSLALIALKLKEAFGLDDVNKLPLSFDIAWYEQKAVLVLLALLYLGFK
                     NIRLGPTIPGFLSPHIVELLVENFGIKGIASPDEDIASMMVGA"
     gene            13160..13801
                     /locus_tag="PLS88_03705"
     CDS             13160..13801
                     /locus_tag="PLS88_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOU06487.1"
                     /translation="MDELHAKLYQARGLRTVSALQKNAFDALYVSTAAEATEKVLEFI
                     YEGAKVGFGGSMTIKSMNIQELAAKKGAIILDHNKPGLGADEKMQILRSQLTCDVFIS
                     SANAITMEGFIFNVDGNGNRVAALSFGPKKNVIVAGMNKVVANLDEAHERLRAYASPL
                     NNIRLEKPNPCVTQGYCADCVLPTRICRIYQVLRRKPSLSDFTVILVGEELGF"
     gene            complement(13896..15257)
                     /locus_tag="PLS88_03710"
     CDS             complement(13896..15257)
                     /locus_tag="PLS88_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU06488.1"
                     /translation="MAQTISKKRRELSRKAVLLWALIALVLSAITNGYHYSLEGAKGL
                     YVWHPLIILKILLYMGLGPITVAILSLPLEKLANKWPNIALRWISFIGSLLVSIVSIA
                     LLAFLIAVPRMSSLEPARLKLIDPSKGIFAQESYVAPSFSKPRNNGANPANSADSTIF
                     LESGGSADSAISSDSMLLASVDGPILRLSFSSDPHWGAETANASARASILQELANRRP
                     DAFFLLGDTVETGNSSTQWNFALSDLEALSPDISLRPIMGNHDTLFGGQYLYKKAFWP
                     KDFKSDSGSPYYWSINSGPATIVALDLPWGTEMFGSRQKAWLEKTLADADPQKALIVI
                     SHSYFYASGYKDPDFGTPWYDHYQNIPALVPLFEKYGVDLVVSGHNHYQELLSHNGVT
                     YAIVGSMGGIPDPEPSYRSPCSQWIEAGGYGWLDVEVFPGQLTLTFRSETGQERQSTT
                     IAY"
     gene            15428..16945
                     /locus_tag="PLS88_03715"
     CDS             15428..16945
                     /locus_tag="PLS88_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOU06489.1"
                     /translation="MTRRIGKIAVIIMAVFALTFTGMLSAQEKLPGNVAITFAHTNDM
                     HGRIVESSDVIGFPKIYSAVQQLKAANPNTLLIDAGDTFHGLPVVNIDKGETAVKLMN
                     ELGYTYMTTGNHDYNYGFDRLIELSKMAKFKILAANVYKDGERVFPAYDIRDIGGVRV
                     AFFGLATPETAYKADPKGIEGVTFGDPIVEAKLIAAELAGKYDVLVCISHIGIDDSSD
                     PTSITIAKFVPQIDVIIDGHSHSTLEDIEKKNTTDTLITSTGSYGAGLGVVDIVISPD
                     RKVVSKTARTITPKNSPDLKGDPAIAKALDDLVKAQEAVLSQVVGRTEIKLEGTREIV
                     RTQQSNLGTLIANSMLYVTGADFALMNGGGIRDSIPVGNITMKQIYTVQPFGNFIQTG
                     KVLGKEIDAILENGVGKLPAPDGRFPHVANLTYTLDASKPAGDRVSNIMIGGEPIDPE
                     KEYTIATLNFIFNGGDDYRMLVGKAQNDYPTDAEVFMAYIKYLGTITNDNIVFKK"
     gene            complement(17187..17942)
                     /locus_tag="PLS88_03720"
     CDS             complement(17187..17942)
                     /locus_tag="PLS88_03720"
                     /inference="COORDINATES: protein motif:HMM:NF022789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HOU06490.1"
                     /translation="MLAGAWISGATLLGEKLGSKRAGLITNLPSNILLSLIFMALTLG
                     PDYAAATTKGVPVGMTIDTLFTFIFILVAKKGLGKAISISLIVWAITAYMFIELIPPL
                     GMGGAIALYVLVTSLCFILAERVLRIRAVPHKDTTFSWKQIVLRALFAGTVVAGAVTI
                     AQFAPPYMTGILATFPAVLSSTLVIFTLSQGTDFARATGKILILSSSNIIIYAWIAGL
                     TFPSLGPWIGTVLSFAASIAYVALLGKLIAKIK"
     gene            complement(17994..18887)
                     /locus_tag="PLS88_03725"
     CDS             complement(17994..18887)
                     /locus_tag="PLS88_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU06491.1"
                     /translation="MIYTEQFDIAFLGQYTKDKIVDKNGSRIVNGGAYYYGAAAAATM
                     GLRVAAITRLAQEDFPSFSPLVERGVMVKAIPTQQSTCLKLFYPSDNPDERILSVTSI
                     AEPFEPSDVESVSARVWSIGASIRGEVSLEVLKTIKAKNARVGLDAQGFVRIVRDGQL
                     IYDSDWPEKEEILSLVDVFKADVVEAEILTGTRDLHEAARALARFGVPEFVLTHGSGV
                     VVYSEGSFFEAPFKPRTMIGRSGRGDTCLASYLSARISMPPKEATLWAAALTSLKMEV
                     LGPFSGTREDVERAIRERYKI"
     gene            complement(18884..20020)
                     /locus_tag="PLS88_03730"
     CDS             complement(18884..20020)
                     /locus_tag="PLS88_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06492.1"
                     /translation="MSERDFSARSSNVTLINLTKRFKSLDGSGEVLAVDDVNLEVKTG
                     ELVTILGPSGCGKTTTLRMIAGFEYPTSGSILIGDKDVAKIPPNKRGLSMVFQSYALF
                     PHLSIYENVAYGLRVQKLPASEIRERTERALDLMQLTTMAKRYPSQVSGGQQQRIALA
                     RAIVIEPSVLLFDEPLSNLDAKLREYMRDELRKLQKRLGITSLYVTHDQSEAMAISDR
                     VVIMKEGRIRQVGTPREIYAFPQSRFVADFMGKANFLEVEVLGTNGESARIDIGGKTF
                     IVPRAGRVRPNSGSALLVVRPEALRLISLANVENEIPLKGCVDRFTYFGNIARYEVAT
                     ENGPLLIESYNPEASSIFEEGDNVGVLIDFESVRLLPPEDKEQQ"
     gene            complement(20007..21698)
                     /locus_tag="PLS88_03735"
     CDS             complement(20007..21698)
                     /locus_tag="PLS88_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU06493.1"
                     /translation="MRENTRLSVAHLVRDPVLLFLVILVFVGLFLFIILPLGKIFAYS
                     VTNNDGRLSFQAAWDMLKSRSYLEPLKNSMLLGITTGILATIIGYVFAYALTRVDMPL
                     KGFFKTIATIPIISPPFILSLSMIFLFGRNGLITRRLLGIEDANVYGMHSLVVVQTIS
                     FFPIAYLTLTGTLSKLNPAVEDAALNLGASRARIFRTVTLPLSVPGILSSFLLVFIQS
                     MEDFSNPAVISGSFSTLAVEAYRTITGMYDMRGGSLMALLLLIPTLIAFLLQKYWLSG
                     KSFVTVTGKPTISQQQSKDPKLVWPLFALCMLISLVILLFYGTVLVGAFVKVWGINFS
                     FTWAHFKYVMTLGWIPLRNSVVLALASTPIAGLLGMIIAFLVVRKEFPGRRAMEFISM
                     LTFAVPGTVVGIGYILAFNDKPFMWTGSAFLLIMAFAFRNMPVGIEAGTSTLLQIDRS
                     IEEASTILGATGAVTFRRISLPMLKQAFFSGLVYSFVRAMTAVSTVIFLISPRWNLAT
                     ISIFSLFESSKYSDAAAYIIVMILFIIVAIGLLNLLVSFLGRGGPSASGGTSERA"
     gene            complement(21813..22844)
                     /locus_tag="PLS88_03740"
     CDS             complement(21813..22844)
                     /locus_tag="PLS88_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU06494.1"
                     /translation="MRKIFLSMLVLFCLLPLMSISAQATPEYKSLTVYTALPESELPT
                     YFSQFEKDTGIKIQYVRLSAGELLARIRAEKNNPQATVWFGGSYDNFVPAIDEGLLEA
                     YQSPELANIPKVYWDAKGYANPFYVGAIGFACNTDWFKKKGLAYPASWDDLLKPEFKG
                     QISMAHPSTSGTSYTILATIVQMRGEEATWKYFTALNQNIRQYTKSGTTPPMDVGLGE
                     AAIGITFSHDGLKPAFEGYPVALSFPKDGTGYEIGCMALIKNGPSKEVANGKRFIDWM
                     LSKRGQDLFETSKSFRIPVNSQATPPKGSISVDSLKVINYDPVWAGQNRNRLIEQFNK
                     VVAAASNLK"
     gene            22982..23869
                     /locus_tag="PLS88_03745"
     CDS             22982..23869
                     /locus_tag="PLS88_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU06495.1"
                     /translation="MQYDIVMMGHISKDIIIDETGNESKLYGGALLYSSIAAARAGAH
                     VLAITKVASEDFCALDIAKEQSGIKILTIRSPKTTSIRNVFLSADHERRETSLLSRAE
                     PFSAADIPLNAETKIIDLAGLFVGELPDNLIEELAPRGAIAVDAQGLLREAQPDGRML
                     FRDWKNKHHYMPFVSYFKADAAEAEILTGLPDREEAARKIAAWGRNREGQEPEVMITY
                     NTEVIVLVDGRIYRAPYTPSNLSGRTGRGDTTFAAYLAWRLGHDPQESVRFAAALCSI
                     KMEKPGPFSGTLEDVFARM"
     gene            complement(23962..26394)
                     /locus_tag="PLS88_03750"
     CDS             complement(23962..26394)
                     /locus_tag="PLS88_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOU06496.1"
                     /translation="MVETKKTDSRTTVQGAKDYSLNRSAKVLRDEVRRIIARAQSEHR
                     EALTELEGMSILAAMGVRTPRYWLVQSAKEFLEKIGANGAMDAPIQGPFSGSKAVIKI
                     ISSQVLHKTEVQGIEIVDNTVAAIADSLCRMEARFKNVSRDGFTVNEFIAFEPKLGHE
                     MIFGYRFTHDFGPIVSFGPGGIYTEYLASKFKPGSANLFLSPRVTNSSALESLLENNV
                     IYGLLCAGLRNTTPELDEKSLVEAIQRFIDSADALSAAGISEFEVNPMVLSKTGELVA
                     LDCLATLRDFAAPAPINASVQSSAQNLIQGLVRDSEGLPINLAQQTRPVDQIARILEP
                     SSVAIIGVSEKTINNGRIILRNLLENGFDISRIYLIKPGVQWIDGCRCVADVQSLPEK
                     VDLFVMVIPALGIPSTLADIARHDKAWSVLLIPGGLEEKEGSESIVAEMNRALAEARA
                     QGKGPLINGGNCLGIRSVPGKYNTLFIPEYKLPMPKGKVEPLAVISQSGAFAITRISK
                     HPNINAKYAITIGNQMDLTVGDYLDYLAQDSEIHVFAVYSEGFKPLDGEKALKATKKI
                     TESGRSVILYRGGRTLAGVGAAASHTASIAGDYPVTWQLFSQAGAIVCESLDEFDDAI
                     TLFTLLDSKRAKGKRLAAVSNGGYECVAIADNLGEMMLSSFSERTKTELEIIFKDARI
                     TEIVDIHNPLDLTPMADDAAYERALRAVLLDPETDLGIVGIIPLTAMLNTLASNPLSH
                     SEDILRPNSLPERLGRLASEIDKPFIAVVDSGPLFDPFCVELEKRGIPTFRAADRALR
                     MLERWRKVHYKK"
     gene            complement(26387..>27047)
                     /locus_tag="PLS88_03755"
     CDS             complement(26387..>27047)
                     /locus_tag="PLS88_03755"
                     /inference="COORDINATES: protein motif:HMM:NF005324.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOU06497.1"
                     /translation="RRALLRWCLGHHNVPGMHRMAEESEAIMTCDIILCSVGGQGGLS
                     ASIILARAAMAAGFQVKQSEIHGMSQRGGEVLAHLRIADKEIYSPTISKGKADIVFAF
                     EPLEALRHLSWISPSNGTIVSATAPVLNIPNYPDLEIVLAEIRKIPRSRLIDAEDIAR
                     QAGNVRTANLVLIGAAMDLLPVSPNAIEKEIIELFSRKGELVIQANLKAFEAGRALYH
                     G"
BASE COUNT         7168 a         6168 c         6784 g         6927 t
ORIGIN      
        1 atgttgcggt tgaaaccggc aatttagtta ttcgtttata atatagcaaa tttttggcca
       61 aaggtgaatt tgagcgatat ttgctatttt atttggctac aatcgcacaa gtctgcaaat
      121 atttattttt atgattttaa accagtgtct ttgtgtcgat tcgacggcta actcgattgg
      181 ctcgataaag tactacatta attgcttaaa atttgcgata attaataatt taggcaagga
      241 gaaaaagcaa agtgccagaa ggcttgcgta gaataaagga gagagaaaag cgttggtata
      301 gccgtctggc ggagggtcta gcaagttttt ttattgctct acaagttgca ctaaggcgct
      361 tactcgcgtg gggaaggact cgcctagtct ttgctatgat cccacaaagc gaaaaaccct
      421 cccggaagtt cgagattcgc cgtttttctc ttttggcaat agttttggtt atagtactct
      481 cggttagcgc cgcaatcatt tcaataggag cctatagtgc aaaatcagta agaataatct
      541 ctctggaagc acaactttcc gatgcatata aaagtatcga tcgaatgcgg gaatctaacg
      601 agaacctact ttcggagacg gctaaattcg aggaaaagct tgagcaggta atagagattg
      661 cgcagagcaa gggaaagccc acagttaagc agagtacgag cgaagagata ctcaaggttg
      721 caggcctgga tgatccaact ttgggacagt tgtccaatga tccagtgagc cgtgatctcc
      781 taaagctcaa tagcatcgca gatgccttgg acaagagcct aggagcgcta gatgatatcg
      841 cgaagatgat tgcgagccaa aaggatataa tgacagaagt gccaaacatc tggccaatca
      901 aggggaacct ggggcacata accatgtatt ttgggcagaa tgagaatcct ttttctacag
      961 gacaatggta tttgcacaca ggtatcgata tttcgactaa ccgaattgga gatgcggtcc
     1021 ttgccacggc agatggcaag gtcatcgaca tacattatga ttcttctctg ggcaattcca
     1081 tcacaatcca gcatagccac ggttttacaa cgcgatatgg gcatctccga agctttgctg
     1141 ttaagaaagg ccagcgggtc tctcaaggcg atgtaattgg ctatcttgga aatactggga
     1201 aaacaacggg gccacatctt cactacgagg tatatctggg tacgagcctt attgatcctc
     1261 tgcgttttct gagtatacgc aaggagtacc atgagtagac gcgatttttc ggattgtatg
     1321 ataggagaag gttctaaggt agatgggact atagatgctc caggcatggt gcgcattgat
     1381 ggagaattct caggcgatat aataagcgcc tcttctgtga taatctcgaa aaatgccgta
     1441 gttcatgctc aaataaatgc tcggcagctc gtggtcgcag gcatattctc cggtagcgca
     1501 attatcgaag ggggagtgcg ctatgcagag actgccataa tcgaagcgga ttgcacgggc
     1561 agatttcttg ttgtagaggc aggagccatg gtaaatgggc agtttgcgcg catagatgct
     1621 gaggactaat actagggagc tacataggca taaggtctgt gaagctgcaa tgttacgata
     1681 tagtttgtat caggagtaaa taatgtctat tgaatataat gatcatgagc tacctgaaga
     1741 tatactcgat atccttgggg aggaggagtt cgaaagccct aaggcattga aacctaagcc
     1801 aaaagacacc gagaatttcc ctcagcctat ttaccggcta aagactttac attcgaacga
     1861 gacttttcat gcctactatg tatcgttgga aaataatgaa cattttatga cgctgtgcga
     1921 acttgaggct gctctagcag gtccgcgtca agcaaataat ggtacttcgc cttctgaaga
     1981 ggcctggaat tgcaagcatg gttcagctag tgcggtaacg gaggctcttc aagcagaggc
     2041 cattcaagaa gaggctcttc aagcagaggc cattcaagaa aaatccattc aagaaatgtc
     2101 cattcaagaa gagggtattc aggcagaggt tattcaagca gatgctattc atgcgaagag
     2161 cgagctcatt cccaatagag cgcttgtcat tgcgcccacc aagtatggaa aggatttggt
     2221 gatggtgctt ggagtggtgc acgatctctc catggtgaat gcagatgagc tggttgcaat
     2281 agaaagaccg gccacggtcg atgattacag gcgctatcta gagaatctcg agcatgaaga
     2341 agaagccttc tccaaatgca gggaatgcat agaagcccac aatcttccga tgaagcttat
     2401 ctcggcgcat tgtcttttgg aggatagcaa gatattgttt ttctttaccg ccgagagtcg
     2461 cgtggatttt cgcgacctgg tcaaggattt ggtcggaatt tttcatgcgc gaatcgagct
     2521 aagacaaatt ggagtcaggg atgaggctcg cgttacaggg ggcgcagggg tctgtgggcg
     2581 cattctctgc tgccatggcc tttcggataa gctcaatccc gtttcgataa agatggcaaa
     2641 agatcagaat ctctctctga attcggtcaa gatttcgggt ccatgcggaa ggctcctctg
     2701 ctgtctaagc tatgagcacc aattctacag agatgctcgt cgcgagctgc cgaacgaagg
     2761 cgtccgcttc acctatgatg gaattctatt caaggttatc gaagtcaatg cgcttctgaa
     2821 caaaattcgg atctccggag aagatggtcg gatcctcgat atggatgcag ccaggctaaa
     2881 gtacctggat ggccactgga cggtcatcga agaagataat tgaagaaaac tagaagaaga
     2941 gacgactaag cggagataat attcctaatc cacaattctt atgtgcagct ctttgagctg
     3001 gcgttcgtcg acttcgtttg gacattgatc cattgggctc atcgcaaagg agtttttagg
     3061 aaacgcgatt acttccttga ttgaggtttc gccagccatg accattacta gcctatcgaa
     3121 gcctggtgct atgccgccat ggggtggtgg accatatttg aaggcttcaa ggagaaaccc
     3181 gaatttgcgc tgggcttctt catcgctaag gccaactatg cggaagatcc gcttctgcag
     3241 ctcggggtca tggatccgga tggatccgga agccacttcg tatccattca gaactaagtc
     3301 atatagatcg cccttgaccg aacccgggtc ggcttcgaga gtctcatggt actgagcttg
     3361 gggcattgtg aacatgtgat gggcagcttg ccagcgattt tcttcttcgt tccactcgaa
     3421 tagtggaaaa tcgactatcc aagcaaaagc aaatacttcg ggatcgcaga gatctaggtc
     3481 tttaccaagc tggcttcgta cggctccgag agcgctgcag gcgatattct ttttctcgtc
     3541 tgctacaagg aggatgagat cgcctggctt tgcactaagt ccttgtgcta ccagcgcatg
     3601 gtccgcaaag aatttggaga caccaccttc aagagtggcg ctctcgctca ctttcatcca
     3661 ggcaaggcca tgagccttgt ataccttagt cttctcttcg agctcctcga tctggcgtcg
     3721 agaatagtca gctttgccag gaacaaccag cgccttcact gcaccgcctt ttgcgagtgc
     3781 attatggaag gcctggaagc tgcaaaagtc atcgacaaaa ggagcaaaat cgactagctc
     3841 cataccgaaa cgcagatcgg gtttatcggt tccaaagcga tccatcgcgt cttcgtaaga
     3901 tagtcgcaca aattgctttg gaagctcgac accaagcgtc tttttgaaga gatgccccat
     3961 catgccttcc acaaggctaa gcacatcatc tctagttaca aagctcatct ctatatcgat
     4021 ttgggtgaac tcgggctgac ggtctccgcg tgaatcctcg tcgcgatagc aacgagcgag
     4081 ctgaaaatac ttatcgaaac ccgagatcat aaggagttgc ttatagagct ggggagactg
     4141 aggcagggca tagaatttgc ctggatagag ccgcgacggc acaagatagt cccgtgcgcc
     4201 ttccggtgtg gagcggatga atgtaggcgt ttctatttcg tagaagccct tgccaacaag
     4261 gtattcgcgc accgcgaaag ccgcttcgtg tcgcaggcga atctttctct gcatcgagaa
     4321 cgagcgaagg tcgagatatc tataggtaag gcgcatatct tccttggcgt cggtctgttc
     4381 atcgatcatg aaaggaagtg aagagcagcg cgaaagaatc tgaatattct ctgcttttac
     4441 ctctatctcg ccggtgggca ttgaagggtt taccatggta tctggccgag gccgaaccat
     4501 accgcggatc gaaacgcagt attccatctt tagttcgttg gctatatcca agagctcctg
     4561 ggcggaatcc acatctacca ctacttgcgt aaccccatag cgatcgcgaa tgttgatgaa
     4621 tgtaatgccg ccatgatcgc gcttgcgatg cacccatcca ttgagtacta cagtttttcc
     4681 tatgtgcgcg gcacgaagct cgccacaagt gaccgttcgc tgttcgaatt gcatattgcg
     4741 cctattctat atcggggcat aaacatgggc aagatggact atacatgagt gtgcggttaa
     4801 tttatgcggg aatggctgtc tcttttgtga tggcaaattc ccatgtataa taattcaaag
     4861 ccatttttgg gttttgcagt ttttaaattc tgcaaaagag gaggtatacg atgaagagat
     4921 tttatgtatt tggattgatt ctgctcattc tagttccagc cggctttgca gcgccaaaat
     4981 caaaagtcaa gatagcgctg atagtcgaat ccacggtgga tgacaagggc tggtgccaat
     5041 cgatgcacga tgctatcgct gctgtacaga agaaatatgg ttcatctcta gttgaataca
     5101 actacagcga gaagatgaag cccgtagacg caggttctgc cgcccgccag tatgtggcaa
     5161 aaggtttcga tatcatcata ggacatgggg ctcagtataa taatgtaatc ctcgaaatgg
     5221 ccgaggaatt tccaaagacg acctttgcat tcgggacatc aggggaaatt ggtcccaaaa
     5281 atgtctttac ctacatgccc caaagcgaag aaacaggcta tgtaaacggc gttatagctg
     5341 gcttagtgac caagacaaac ataataggca tcgtagggcc agtcgatggt ggagatgcag
     5401 cgcgctataa taggggcttc atccttggcg tgcggtctgt aaacccaaag gcacaagtac
     5461 aggttgccta tacgggcagc tttggcgatt tcgtcaaggc ggccgagctc gcccatacgc
     5521 agataaaagc aggggcagat gtgctaaccg gttctgcgca gcaggcgcta ggtgctctca
     5581 gagcagttgc agagtacaaa gacaaggccg tttggtggct tggtcaggac acaatgcagt
     5641 taacgattcc ggagagttac aaggtaattg cggcaggtgg ctatgcttat gagcctgttg
     5701 tcgaactgtt tatagataaa cgccaggcgg gtattttagg cggagaatgc atacctctca
     5761 gttttaccaa tggtggcttt gtctatcagt tcaacgacaa agtagggcca attcttagcc
     5821 cagaagtcag aaaagccgca gagaaagcaa aggccgacat aactagcggg aaattaatca
     5881 ttccctggaa ggatgtaaaa taaatcgaat agcggtttca tattgtcatg agcaagccgc
     5941 atagaatact ctccagggga tgtgaatgac caaaacaaaa gacaagatat attctctaga
     6001 agcaaagggt attaccaagc gtttcccggg agttcttgcc tgcgattcaa tcgatctcag
     6061 tattggagaa aacgagatac tcgccatagt aggagagaac ggcgcaggca agacaactct
     6121 catgaatatt ctaatggggc tatatcagcc ggatgaggga gagattcgca tcaatggaag
     6181 aacggtccgt tttcgcacgc ccaatgatgc ctttgcggca gggatcggca tggttcatca
     6241 gaattttatg ctggtaccga acatgactgt agcggaaaat gtagctctcg gatccaggga
     6301 tttccataaa ttcctaaagc tcgacctagc tggggcgaaa aaacgcataa tagaggtatc
     6361 cgagcgcttt ggcctgcctg tccagcccga tacctatgtt tggcagcttt cggttggtga
     6421 acagcagcga gtagagctta tcaagactct ttgttttgga gctcgatttc tcattctcga
     6481 tgaaccgaca agcgcgctta cgcctcagga aaccgaggag cttattgttc ttctcaaacg
     6541 aatggctcag gagatgtcga ttatctttat tagccacaag cttgccgagg ttaccgccct
     6601 ttcggataag gtggtaattc ttcgccatgg tagagttgtc ttttcgggga cgacctcgga
     6661 gcagaccccc tcgggccttg cggcgcttat gacaggacat gaggtagttt tgcccaagaa
     6721 cgagagcagc gaagaatgtg gagatgtatg tttcgattta gagaatgtct atgccaagtc
     6781 cgatcgtgga tatatggcac tcgatggagt tagtttttcc attcgacagg gcgaaatctt
     6841 ggggcttgcc ggtgtgtcgg gcaatggaca gaaggaactc gccgaggcga tagccgggct
     6901 cagaaaaatc gaatcgggcc gtatcagtct ttttggaaaa gacttgacga atttgcggcc
     6961 gcgcgaacgc atagatcaag ggcttggcta tattccagag gataggaact cggagggcat
     7021 agttccgtca ttttccgtaa aagagaactt tattctcaaa gattttgctt cgcctgattt
     7081 tgccaatggt ccctttctga aactaaagaa cggagagcaa aaggccgtcg aactaaagga
     7141 aaaattcgat attcgctgcc caagcgtgac cacgccggca ggtgccctct ctggaggcaa
     7201 tattcaaaag gtgattctcg ccagagaact tgccagaaat ccaaaagtcc ttatagcggt
     7261 atatcccaca agagggctag atatgggagc cgaagagtac attcacaaga agctgctaga
     7321 aatgcggagc cagggtacgg caattcttct catctccgaa gagctcgagg aaataatgaa
     7381 cctatcggat agaattgcag tcatttataa aggaaagata cttcgaattc tgacctccag
     7441 agaagcttct atgcgcagca ttggcttgtt gatggcggga atcaccgaac cgatggcgat
     7501 ggaggcagga cagtgaaatc gactatcaga attgtttaca ttgtgttgat ttctctggct
     7561 gctgcagttg tagccatggc gattgccgcg atcgtgcttg gcattctcgg agctagcgta
     7621 acacagacct tcaagataat tttgaccgag ccgttaaagg atattgtagg aattaccgaa
     7681 atcctcgtgc gagccatacc tctcacgctg gtgagcctag gcatcgcgat atccttccga
     7741 tcgggaattt tgaatattgg ggcggaaggc cagattcaga tgggagtaat tgctggggca
     7801 gcggtggcta ttgcattgcc aaatatgcct aagtttttgc ttgtaccgct cgcgctgctt
     7861 gcaggcacca taggtggggc tctttggagc tttgttccag ggtatctgcg cgccgtgctt
     7921 ggggtaaacg aaatcttatc tacggttatg atgaactata ttgcggtaca gttttatggc
     7981 tttttacttc gcggcccaat gatagatcct gccgagctgc aggtcggttc aggcacgcct
     8041 cagtcggtaa ggcttcccag gtctgcatgg atcgatcgaa tcatcccggg aacgcgtctg
     8101 cactggggaa ttgcaatcgc gctcgtctta gcattagttg tgtatattct tctttggaga
     8161 acgacctggg gatttagaat gagggcagct ggagcatcgc caaaatccgc acggtatgcg
     8221 gggataaatg tcgaacgctg tcttttgatg gccatggtgt tggccggggc ttttgctggg
     8281 cttgcagggg ctgtggaggt aaccggcgtc cataggcggg ctatcgaagg aatttcttcg
     8341 aactatggct tttccggtgt cgtagttgcg ctctttggag gtttgcatcc ggctggaata
     8401 attccttccg cggttttctt tggactattg cttgttgggg ccgatatgac tcagaggatg
     8461 atttccattc ctgcaaatat ggtactcgtg cttcagggtg ctgtaatact aagcattatc
     8521 tcggctaaga tgctcatcaa cgacccttat ctctacgaca gatttgcgcg gcgattcaca
     8581 tcgggcttct ttggctctcg ccgtctgaaa aagccaaagg aaggtagggc atgagttttt
     8641 tcatgtttgt caacaatgtc ctctcgatag gaataccttt ttctgtggct cttctattgg
     8701 ctgcgctggg ggagatgttc aaccagaggg cgggagtgtt caatctaggc tgcgaaggga
     8761 taatgtcgat gggagccttc ataggttttc ttccggcata ttatcttcaa gagacagcgc
     8821 ttgcgcctta tgcaaacatg atagggataa tgggagcggc aggcattggt gtacttcttg
     8881 ggattttgta cgcactggtg acagtcacat tcaaagcacc tcaaggtatt gcaggtatcg
     8941 ggcttcagat gctgggctgg ggaatcgcag ggacactttt ccgattcttc atcgggggag
     9001 taacgggtgt tccgggattg ccaaataggc cgatacctct attatcgaag attccgtttt
     9061 tagggcctat tatcttttct atgaatgggc ttgcatactt tgctttctta tttgtgccat
     9121 tcagctatta cctgctcttc aagacaccct gggggctcaa ggtgcgcgct gtaggtacct
     9181 atccgagggc tgccgatact atgggtattc cagtcagcaa gacgcgatac caagcgcttg
     9241 cgctaggggg gctattggct ggattagcgg gggcctatct ttctttgggt caagccaaga
     9301 tgtttgcgga cgatatcgtg gccggaagag ggttcattgc ggttgcgctt gtatactttg
     9361 gtcaatggaa tcctctgggt atcctaggag gtgcattgct ttttagcatt gctcaatctc
     9421 tgcagttggc tatccaggtg ttgggaatca atttccccta tgagtttgcc gtaatgctgc
     9481 cttatgtgct cgtaatagtc gtgctcgcca taactagagg ttcggccata aagggtccaa
     9541 cagagctggg caaggcctat gatagagaga agagaatatg acggagaaaa gccttcctga
     9601 ctttaggatt gagacacttg ggaattgccc ggtgaattct cctcttggcc tggcaagtgc
     9661 tccaggagaa gggagacctg tttttgtgcc cgaagatcgc tttatctact acaacatcga
     9721 gcgagatata agaaaaaaga ctggtagttc cgcttcgaag gggaaaaagg agcagggctg
     9781 caactcttcg gtagaaaaag tcgaccagct cctcgaagtg gcgggtcctc gccagcaaat
     9841 ctattttagc ccatcgcatg tccatgctgg tattgtaacc tgcggggggc tctgtcccgg
     9901 gctcaacgat gtgatccgct ctgtcgtgag aagtctttgg tatctctatg gagttcgcag
     9961 aatatcgggc atcaggtatg gttataaggg ctttttgcct gaatatggat tcggggtagt
    10021 tccccttgac ccagatattg tcgatgacat ccacaaaatt gggggtacga tgcttggcac
    10081 ttcacgtgga ggtggcgagc ggacctcgga tattgtggat accatagaac agctcaatct
    10141 aaatatgctc tttgcgatcg gaggagacgg tacccaaaag ggagctctgg ccatagccga
    10201 agagatggag cgcagaggtc ttagaatcgc cgttgtagga attcccaaga caattgacaa
    10261 cgacatcgaa ttcgtagaga agacctttgg tttcgatacg gctgtggtaa aggcttccga
    10321 ggcggtttcg gcagctcaca tggaggcaca ttcccagata aacggcatag gtcttgttaa
    10381 gcttatgggt agagactcgg ggttcatagc cgtccagacc gtcctcgccg ttcatgaagc
    10441 caatttcgtt ttgattccgg aggtaccatt tgatctcgaa ggaccgaatg gttttctttc
    10501 ggcgctggaa gatcggctcg aaaggcgagg gcatgcggtg atagtggttg ccgaaggcgc
    10561 tgggcagcat tttttcgatc aaccagttac ctatgatgcc tcgggcaata agaagcttgg
    10621 tgacattggc ctctatttgc gagatcagat tcaagctcat ttttcagaga aaaacataga
    10681 gataaatctc aagtatatag atccgagcta catgatccgt tcggctattg cagagcccat
    10741 agattcaatg tactgcgaaa gacttggcaa caatgcggtg catgcagcta tggccggcaa
    10801 gaccaaaatg cttattgggc tcgtcaataa tgaattcgtc catattccga ttcgagccgc
    10861 gacatcgagg cgaaaacttg taaacccaga gggcagtctt tggcgcgatg ccatcgaggc
    10921 tacgcaacaa cccttttcca tgctgaaaaa agatcaaaaa taggatatcg atgtcttgaa
    10981 tctttctgga agtttgatat aataggatat cgatatccta ttaaggactc tattctgaca
    11041 tggatagaat ttttgctatt tcggacaccg tgaataccgc ggtgcatgcg cttgcgcttg
    11101 cggccgcaag cggcggaagc atatctgcta gcaaggctgc aaaagaactc gatgtgtcac
    11161 caagctattt tgccaaaata ttgcaaaaac ttgcttcata tgggctactg gttccctcaa
    11221 gaggcaagcg tggcggatat agcctctcta aacctgccga ggctatttcc tgcctagatg
    11281 tcctaatagc ccttgaaggt gaatttccaa aacgagaatg cctttttcaa cattctatat
    11341 gccgaaaaaa aacctgtgct ttgcgcattc tgtgcgaagt tacatcgacc aatctgcgcc
    11401 atgctctcga aaatacgact attgccgccg ttgcgcggag cttttagata tcaagatctg
    11461 caatttacag gaggacagcg atgttttgta atcaatgcca agaaacattg cgaaacagcg
    11521 gatgcaatat cgccaaaggc atgtgtggca aaaccgctga aacagctcat ctccaagacc
    11581 tattgctcta tgtttgcaag ggaataggat attgggccga taacgcacga gccgttggct
    11641 attttcccga agaggacgcc ttttttgtaa caagaatgct ctttgcgacc ataactaatg
    11701 caaattttgc tccaagcgac ttcgagcatt ggattatgga ggctctaaag cgccgtgacc
    11761 gaataagaca ggagttctct gagagaggcg gaaagatcga tgcaattgtg cccgacgctg
    11821 caacatggaa tgccgtcgat ctcgttgcaa ttcgaatggc cgcagcacaa gataaaggtg
    11881 ggcttctcga aattaccgat gaggacgttc gtgcgctcaa ggctctagtg ctctatggcc
    11941 tcaaaggaat ggcggcttat ttggagcatg cttatgtcct gggcgtttcc tcgaaggaag
    12001 tcttcgaatt catgttcagt gctcttgcaa agctcgccga cgatacgata gataaagaag
    12061 cgctcatcca gctcgtccta gatactggaa agtatggcgt agaggctatg gcgctcttgg
    12121 ataaggccaa cacgggcagc tatggaagcc cacgtataac caaggtatct accggtgttc
    12181 gcaatcgtcc gggcatatta atttcggggc atgatttgcg cgacctgcac gaccttctcg
    12241 agcagacaaa gggtaccggc atcgatgtct atacccatgg cgaaatgctt cctgcacact
    12301 attatccatt cttcgagaaa tacgacaatc tatatggcaa ttacggcaat gcatggtgga
    12361 agcaggattc cgaaatcgaa aaattccatg ggcccgttct atttactagc aactgccttg
    12421 tacccccaaa agaaagttat aagaatagaa tctttaccac gggagtagtc ggtttcgaag
    12481 gtgttactca tattcccgac cgagagcctg gcacaatgaa ggatttttcg gcgatcatcg
    12541 agatggcaaa aacctgttca cctcctgagg cgctagaaga tggggagatc gttggcggat
    12601 ttgcgcatga ccaggtcttt gctcttgccg ataaagtcgt cgaagcggtc aaaaatggcg
    12661 cgataaagcg attcattgtc atggcaggtt gcgatggccg tcagcctaag cgctcatatt
    12721 acaccgaagt tgcccaaaaa cttccgaagg atacagtcat tttgactgct ggctgtgcca
    12781 aatatcggta taacaagcta aacctcggcg atataagcgg tattccgaga gtgctggacg
    12841 caggtcaatg caacgattcc tattcgctcg cgcttatcgc gctaaagctc aaggaagcat
    12901 ttgggctaga tgatgtgaac aaacttccgc tttccttcga tatcgcttgg tatgaacaga
    12961 aggcagttct tgttctcctt gctcttctat atctaggatt caagaatata cggcttggcc
    13021 ctacaatccc cggatttctg tcgccacaca tagtggagct tctagtggaa aatttcggaa
    13081 taaaagggat cgcttcgcct gacgaggata ttgcttcgat gatggtagga gcataaagta
    13141 atgtcgagga ggatgctcga tggacgaact gcacgcaaaa ctataccaag ctagagggtt
    13201 aagaaccgtt tcagcgctcc aaaagaacgc tttcgatgcc ctatatgtgt ctacagctgc
    13261 cgaagctaca gaaaaagtgc tcgaatttat atacgaaggc gctaaggttg gttttggagg
    13321 ctcaatgaca atcaaatcga tgaatatcca agaattggca gcaaaaaaag gagctatcat
    13381 actcgatcac aacaagcctg gccttggcgc agatgaaaaa atgcaaattc ttcgatctca
    13441 gctcacctgc gatgtcttta tctcgagcgc aaatgccatt accatggaag gctttatttt
    13501 caatgtcgac gggaatggga accgcgttgc cgcgcttagc tttggtccaa agaaaaatgt
    13561 catcgtggct ggaatgaaca aggttgtcgc aaacctcgat gaggctcacg agaggctaag
    13621 agcatatgct tcacctctca acaatattag gctcgaaaaa cctaatccct gcgttacaca
    13681 gggctattgt gccgattgtg ttctccccac gaggatatgc agaatttatc aggttttaag
    13741 gcgcaagccc agcttgagcg attttacggt gattttggtt ggagaagagc tgggattctg
    13801 aaggcatttc ttggtagcag gtatttcttg ctgattggtc tagggattga gctcaagatc
    13861 gacaatctgt ttagataaga ttagataacg ttggatcaat atgctatcgt cgtgctctgc
    13921 ctctcttggc cggtttcgct gcggaaggtg agcgtcaatt ggccggggaa aacctcgaca
    13981 tcgagccagc catagccccc tgcttcgatc cattgagagc agggcgatcg atagcttggt
    14041 tctggatccg gaatccctcc catagatccg acgattgcat aggtcactcc attatgcgaa
    14101 agcagctctt gataatgatt gtggcccgaa acgactaaat cgacgccata tttctcgaaa
    14161 agtggtacaa gggcaggaat attttggtaa tgatcatacc aaggcgttcc aaaatcgggg
    14221 tctttgtagc cagaagcgta aaaataacta tgcgaaataa caataagagc cttttgaggg
    14281 tcggcgtccg caagcgtttt ttcgagccaa gccttttggc gagagccaaa catctcggtt
    14341 ccccaaggga ggtcaagagc cacgatcgtg gcaggacccg agtttatgct ccagtaatag
    14401 ggcgagcccg aatcgctttt gaaatctttt ggccagaagg ccttcttata taggtattgg
    14461 ccgccaaaga gcgtatcgtg atttcccatg atagggcgca aggagatgtc tggcgataat
    14521 gcttcgagat cagatagcgc aaagttccat tgggtagagc tgttgcctgt ttccacagta
    14581 tcgccgagca aaaagaatgc atctggcctt cggtttgcga gttcctgcaa gatgctggcg
    14641 cgtgcacttg cattcgcggt ttcggcaccc caatgcggat cggaagaaaa agacagccta
    14701 agaattggcc catcgacgga agcaagcaac atggaatcag aagaaatagc agaatcggcg
    14761 gaaccgccag attcgagaaa aatagtggaa tcagcagaat tggcaggatt ggcaccatta
    14821 ttcctgggtt ttgaaaaaga aggggccaca tagctttcct gcgcaaaaat ccctttcgat
    14881 ggatctatga gctttagtct tgcaggttca agcgaactca tcctcggtac ggcgatcaag
    14941 aaggcaagta gcgcaatcga tactatactg accagcaaag agccaatgaa cgatatccat
    15001 ctaagcgcga tgttgggcca tttattagca agcttttcaa gtggcaagga tagtatggca
    15061 acggtaattg gtccaagccc catatagagc aagatcttta gaataataag ggggtgccac
    15121 acatagaggc cttttgctcc ttcaaggcta taatgatagc cattcgtaat agcagagagg
    15181 accaatgcta tcaaagccca aagcagcaca gccttgcgag aaagttctct tctctttttg
    15241 ctaattgttt gtgccatgta gctactatat cagtaatata ttccagcggt aagcggctat
    15301 tggagtcttg attggctttt ttcatgcaag aatagaacaa ttatttcttt ttgcttgtct
    15361 gtagggaaag agctgggtat aataatactg tttcttaaga atacaacaat gaatcaggag
    15421 gtttgcaatg acacgaagaa tcggaaaaat tgccgtgatt atcatggcag tttttgcgct
    15481 aacctttacc ggcatgctgt cggcgcagga gaagctgcct ggcaatgttg cgatcacatt
    15541 tgcgcatacg aatgatatgc acggacgcat tgtcgaaagc agcgatgtaa ttgggttccc
    15601 gaagatttat tcggcagttc agcaactaaa ggccgccaac cccaatacgc ttcttataga
    15661 tgcaggcgac acattccatg gtttacctgt cgtgaatatc gataagggcg agactgctgt
    15721 aaaactcatg aatgagcttg ggtataccta tatgactacc ggcaaccatg attacaacta
    15781 tggattcgat cgcctaatag aactctccaa aatggcaaaa tttaaaatct tggcagccaa
    15841 tgtctataag gatggagaga gagtattccc tgcctatgac attcgcgaca taggcggtgt
    15901 gcgtgtagca ttctttggac ttgcaactcc agagactgcc tacaaagcag atccgaaggg
    15961 catcgagggt gttacatttg gcgacccaat agttgaagca aaactgatag ccgccgagct
    16021 tgcgggcaaa tatgatgtgc tggtctgcat atcgcacatc ggaatcgatg attcgagcga
    16081 tcccacttcg ataacaatag ctaaatttgt gcctcagatc gatgttatta tcgatggaca
    16141 cagccacagc acactcgaag atatcgagaa aaagaatacg acagacaccc tcattacaag
    16201 cacaggttcc tatggagcag ggcttggtgt agtcgacatc gtaataagcc cagatcgaaa
    16261 ggtcgtttcg aagaccgctc gcactattac tccaaagaat tcacctgatc ttaaaggcga
    16321 tccggccatt gcaaaagcgc tggatgacct tgtcaaagca caggaagccg tgctatccca
    16381 ggttgtgggc aggaccgaaa taaaactcga aggcacacga gaaattgtcc gtactcagca
    16441 gagcaatctt ggtacgctaa ttgccaactc aatgctctat gttactggtg ctgatttcgc
    16501 tttgatgaat ggcggtggca ttcgagattc tattcctgta ggcaacatca caatgaaaca
    16561 gatttacacc gtgcagccat ttggtaattt tatccagact ggcaaggtcc ttggcaagga
    16621 gatagatgcg atactagaga acggtgttgg caagctacct gctccagatg gtcgcttccc
    16681 gcatgtagcc aatctcacct atacactcga tgcctcgaaa cctgcaggcg atcgagtctc
    16741 caatatcatg ataggaggtg agccgatcga tccagagaag gaatacacga tcgccactct
    16801 taacttcatc ttcaacggtg gagacgacta tagaatgctc gtaggcaagg ctcagaatga
    16861 ctatccaacc gatgccgaag tctttatggc atatatcaag tatcttggca ccataacgaa
    16921 cgacaacatc gtgttcaaga aataggggaa gcctatacag gccttttgtg aatgttcata
    16981 ggccgacacg aggttttgat tggatttaag ggggctgtcc gatgatttgg gcagtcctct
    17041 tttttataaa ttgtaatcat taggaatccg aaagcggcta ccatttgtca gctgcctttt
    17101 ttattgatag caacttatta ttagtagacg tcttttttac cgccttgtcc attgataact
    17161 cattggtgat tatctttttc gctcatttat ttaatttttg cgataagttt cccaagtaga
    17221 gcaacatagg ctatcgaggc tgcaaaggaa aggacagtgc ctatccaagg tccaagcgat
    17281 ggaaaggtca gtccagctat ccatgcatat atgatgatat tgctcgaaga aagaatgagt
    17341 attttgccag tggctcgcgc aaagtcggtt ccttggctca acgtgaaaat aacgagggtg
    17401 ctcgagagaa cggctggaaa agttgctaaa atgccggtca tatatggagg ggcaaattgc
    17461 gctatagtta ccgcgccagc cacaaccgtt ccagcaaata gagctctcaa gacgatttgt
    17521 ttccaggaaa aagtcgtatc cttgtgtgga actgcgcgaa tacgcaaaac ccgctccgca
    17581 agaataaagc acaaggaagt cactaagaca tagagagcga tcgcgccccc cattccaagc
    17641 ggtggaataa gttcaataaa catataagcc gttattgccc atacgatcaa gctgatagaa
    17701 atggctttcc ccaagccttt ttttgcgact aggataaaaa tgaaagtaaa gagggtatct
    17761 atggtcatac ctaccgggac gcctttggtt gtggcggctg cataatctgg ccccaatgta
    17821 agtgccataa atatcagcga tagaaggata ttcgatggaa gattagtaat cagtcccgct
    17881 cttttgcttc caagcttttc gccaagcagt gtagctccag agatccatgc acctgctagc
    17941 acaaaagaaa gagcaatttt taagacagat ccggatataa ttgcacctcc attttatatt
    18001 ttgtagcgtt ctcgaatagc acgctcgaca tcctcgcgcg tccccgaaaa tggccctaat
    18061 acttccattt taaggcttgt cagcgctgct gcccagaggg tggcttcctt tggcggcatg
    18121 gagatacgcg cgctgaggta agaggctaga caagtgtcac ctcgaccgct tcttcctatc
    18181 atcgtccttg gcttaaatgg tgcttcgaag aaagatcctt cggaatatac gaccacgcca
    18241 cttccatgtg ttagaacgaa ttcgggaacg ccaaatctgg caagggcgcg agcggcttca
    18301 tggagatctc tggttcctgt cagtatctcc gcctctacga catctgcctt gaatacatcc
    18361 acaagggaga gtatttcttc tttttcgggc cagtccgagt catatataag ctgaccatcg
    18421 cgtacgattc gcacaaatcc ctgagcatcg aggcctactc gtgcgttttt ggctttgatc
    18481 gtctttagga cttcgagact cacttctccg cggattgatg ctccaattga ccagactcgt
    18541 gccgaaactg attcgacatc cgaaggctcg aagggctcgg ctatcgaagt aaccgacaga
    18601 atacgctcgt cggggttatc cgatggatag aataatttaa ggcaagtcga ttgttgggta
    18661 ggaatggcct tgaccatcac gcctcgttct acgagcgggc taaaactggg gaaatcttcc
    18721 tgtgcaaggc gtgttatcgc cgctactctc agccccatag tcgccgctgc tgcagcacca
    18781 tagtagtacg caccgccgtt tacaatgcgt gaaccatttt tatcaacgat tttgtccttt
    18841 gtgtactgac ccaagaatgc aatatcgaac tgttctgtat agatcattgt tgttccttgt
    18901 cttcgggcgg tagaagcctt acagattcaa agtcgatcaa aacgccaaca ttgtctccct
    18961 cctcgaagat agaggacgcc tctgggttgt agctctctat aagcaagggg ccattttctg
    19021 tagcaacctc ataacgcgcg atattgccga agtatgtgaa gcgatctacg cagcccttga
    19081 gagggatttc attttcaaca ttagcgagcg atataagcct caaagcctcg ggcctcacta
    19141 ccaacagcgc actaccgcta ttcggcctta ccctgccggc cctcggcact ataaaagtct
    19201 tgcctccaat atctatccgt gcggattcgc cattagtgcc cagcacctcg acctcgagaa
    19261 aattcgcttt gcccataaag tccgcaacga atctcgactg cggaaaggca tagatttcgc
    19321 gaggtgttcc aacctgtcgg atccgtcctt ccttcataat cacgactcta tcggatatgg
    19381 ccattgcctc gctctggtca tgcgttacat aaaggctagt tatccccaac cttttctgta
    19441 gtttccgcag ttcgtcgcgc atgtattcgc gcaatttggc gtctagattc gagagcggct
    19501 catcgaagag gagcacgctg ggttcgatca cgatagcgcg ggctagagca atcctctgct
    19561 gttggccgcc ggatacttgg ctcggatagc gcttggccat tgttgtaagc tgcatgagat
    19621 cgagtgcacg ctccgttcgc tcgcgaattt cgctggccgg caacttttgc acccgcagcc
    19681 cataggccac attctcatag atcgaaagat gcgggaatag agcataactc tgaaaaacca
    19741 tagatagccc gcgcttgttg ggcggaattt ttgctacatc tttatcgcct ataagaatcg
    19801 agccagaagt aggatactca aagccagcta tcattctgag agtcgttgtc ttgccacaac
    19861 ccgaaggccc tagaatcgta actagctcgc cagtcttgac ttcgagattg acatcatcga
    19921 cagccagcac ctcgcctgag ccatcgagac ttttaaatct tttagttaga ttgataagag
    19981 ttacattaga acttcttgca ctaaaatcac gctcgctcac ttgtgcctcc acttgctgaa
    20041 ggaccgcctc ggcctaagaa actaaccaga aggttaagta aaccgatagc aacaattatg
    20101 aaaagaatca tcactatgat ataggccgcg gcatccgaat atttgctcga ttcgaaaagc
    20161 gaaaaaatgc tgattgtagc caaattccag cgcggcgaga taagaaagat cacggtgctc
    20221 accgctgtca tggcgcggac gaaagagtat acaaggccgg agaaaaaagc ctgctttagc
    20281 attggcaagc ttatgcgcct aaaggtgaca gcccctgttg cgccgagtat tgtcgaagcc
    20341 tcctcgatgg atctgtcgat ctgtaagagc gtcgaagtgc ccgcttctat tcccacaggc
    20401 atatttcgga acgcaaatgc cattatcaat agaaaggccg accctgtcca cataaagggc
    20461 ttgtcattga atgcgagtat atatccgatt ccaaccacag tcccagggac cgcgaaagtc
    20521 agcatggaga taaattccat tgcgcgtctg cctgggaatt ctttgcgcac taccaagaaa
    20581 gcgatgatca ttccaagaag ccccgcgatg ggcgtagaag ctagagcaag aacaacggaa
    20641 tttcgcagag gaatccagcc aagcgtcatt acatatttga agtgcgccca ggtaaagcta
    20701 aaattgatac cccagacctt gacaaaagct cctacaagaa ccgttccata gaataggaga
    20761 atcacaagcg aaatcagcat acataatgca aagagtggcc ataccagctt gggatccttg
    20821 ctctgttgct gcgatattgt gggcttgcct gtcactgtca caaaggattt gcccgatagc
    20881 caatattttt gcagcaaaaa ggcgatcaga gtcggtatta gcagcaatag cgccatcaaa
    20941 gaaccgcctc tcatatcgta catgcctgta atggttctat aggcttcgac agcaagcgtg
    21001 gagaaactcc cagaaatgac cgctgggttg ctgaaatcct ccatcgattg aatgaacaca
    21061 agcaggaaag agctcaaaat acctggcacc gaaagcggta gcgtgacagt tcggaaaatt
    21121 cgggctctag aagcgccaag atttagggct gcatcctcca cggcaggatt caatttcgaa
    21181 agagtaccgg tcagagtaag gtaggctatt gggaaaaaag aaattgtctg cacaacgaca
    21241 aggctgtgca ttccatagac atttgcgtcc tcaataccta gaagcctgcg agttattagt
    21301 ccatttcgcc caaaaaggaa gatcatcgat agcgatagga taaatggcgg agagattata
    21361 ggtatcgtcg cgatcgtttt gaaaaacccc tttaggggca tatctacacg agtaagggca
    21421 tatgcaaaga catatccaat gatagttgcg agtatgcctg ttgtgatccc caacaacatg
    21481 gaattcttga gcggttcgag atatgagcga gattttagca tatcccaagc tgcctgaaag
    21541 ctcaatctac catcgttatt cgttacgcta tatgcaaata tctttcccaa gggcaaaatt
    21601 ataaagagaa aaagcccaac aaacacgaga attactaaaa agagaagcac gggatcgcgc
    21661 acaagatgcg ctaccgaaag gcgagtgttt tctctcatga aaatctcaga ttcctttgaa
    21721 atgaagattg tgcaggtata ttaattctgc accggaggcg cgtagagaaa ttattgcgcg
    21781 cctccactaa agctagggct acctgttata ttctacttca ggttgcttgc tgctgcgact
    21841 actttattga attgctcgat caatcgattt ctattctgac cggcccatac aggatcatag
    21901 ttgataacct tgaggctatc gaccgaaatt gagcccttgg gaggagttgc ctgtgaatta
    21961 actggtattc taaaggattt ggaagtctcg aagagatcct gtccacgctt cgacaacatc
    22021 cagtcgataa accgcttacc attggctact tcttttgatg ggccattctt tataagcgcc
    22081 atgcagccga tttcataacc tgtgccatct ttagggaagg ataaggcaac aggatagcct
    22141 tcgaatgcag gcttgaggcc atcatgggag aaggtaattc caatggcagc ttcgccaagg
    22201 cctacatcca taggaggagt cgtccctgat ttggtgtact gacggatgtt ctggtttaga
    22261 gcggtaaagt atttccaagt agcttcttct ccacgcatct ggacgatagt tgcaagaatt
    22321 gtataggaag ttcctgaagt acttgggtgg gccatcgaga tctggccttt gaattcgggc
    22381 ttcaatagat catcccaaga ggcagggtag gcaaggcctt ttttcttgaa ccaatcggta
    22441 ttgcatgcaa agccaattgc tcctacatag aagggattcg catagccctt ggcgtcccag
    22501 tagaccttcg gaatgtttgc aagttctggg gactgatagg cttcaagcag accctcgtcg
    22561 attgctggca caaagttgtc gtaggaaccg ccaaaccaaa ccgtagcttg cgggttgttc
    22621 ttctcggccc ttatacgggc aagcaactcg ccggcagaga gcctgacata ttgaatcttg
    22681 attccggtgt ctttttcgaa ctggctgaaa tatgttggga gctcagattc cggcagagcc
    22741 gtataaacag ttagtgactt gtactctggc gttgcctgcg cgctgatcga catcaagggc
    22801 aacaggcaga acagcactag catcgaaaga aaaatcttgc gcatagattg cctccttata
    22861 tgttcctcgt ctattataga acaatattat agaacaagtt taacaaaatg caactgaaat
    22921 cgtttgcagg atgctaaaaa cgttttcata tatttgctag acaaatgtaa aggagaagcc
    22981 tatgcaatac gacatagtga tgatgggtca tatttccaag gatataataa tcgacgagac
    23041 agggaacgag agtaagctat atggcggagc gctcttgtat tcatctattg cagcggctcg
    23101 agccggagcc catgtgctcg ccatcaccaa ggtcgcttca gaggattttt gcgccctcga
    23161 tatagcgaag gagcagagtg gtataaagat cctgactatc cggagcccca aaactacatc
    23221 gatccgcaat gtattcctat cagcagacca tgaacggaga gagacaagcc ttttatctcg
    23281 agcagaaccg ttttcggcgg cggacattcc tctgaatgca gagacgaaaa taatcgatct
    23341 ggcaggtctt tttgtaggcg aattacctga taatctcata gaagagcttg cgccccgcgg
    23401 tgctatcgcc gtcgatgcgc aaggacttct tcgcgaggct cagcccgatg gcaggatgct
    23461 tttcagggat tggaagaaca agcaccatta catgcctttt gtgagctact tcaaggccga
    23521 tgccgccgaa gctgagattc tcacaggtct tcccgacaga gaagaagctg cacgtaaaat
    23581 agcagcttgg ggcagaaacc gagagggcca ggagcccgag gtcatgatca cttataatac
    23641 cgaagtgatc gtgttagtgg atggaagaat ctatcgtgcg ccctatacgc cttcgaatct
    23701 ctccggaaga acggggcggg gcgacacaac ctttgccgcc tatcttgcct ggcgtcttgg
    23761 ccacgatcca caggaatcgg ttcgatttgc agcagcactc tgctccataa aaatggaaaa
    23821 accagggcct ttttcgggaa ctctcgaaga tgtatttgcg cgtatgtagc gtgcatctga
    23881 gtgcgccttt aattcttaat gccttaatgt cccaatgcct taatgcgcct ttaatagccg
    23941 atatttttat gccttagtgc tttatttctt ataatgcact tttcgccagc gctccagcat
    24001 tcgcaaggct cggtccgcgg cgcgaaaagt tggaattcct cgcttttcca gctcaacgca
    24061 gaaaggatcg aagagcggac cagagtctac aacggcgatg aaaggcttat cgatttcgct
    24121 cgctaacctg ccgagtcgct cgggaagcga attgggccta aggatatctt cactgtgaga
    24181 taaaggattg cttgcaagtg tattcaacat ggctgtgagc gggattatcc caacaatacc
    24241 gaggtcggtt tccgggtcaa gaagtaccgc gcgaagggcc ctttcgtagg cagcgtcatc
    24301 cgccattggg gttagatcga gagggttgtg gatatcgact atctccgtta tgcgcgcatc
    24361 tttgaaaata atttcgagct cggtctttgt cctttcacta aaagaagaca acatcatctc
    24421 gccaaggttg tcggcaatcg caacgcactc atagccacca ttggagacag ctgcaagcct
    24481 tttgccttta gctctcttgc tgtcgagcag tgtaaataat gtaattgcat catcgaattc
    24541 atccagactt tcacaaacga ttgcgccagc ttgagaaaaa agttgccacg taacaggata
    24601 gtcgccggca atgctggcag tatggctcgc cgcagctcca acccctgcta gtgttctgcc
    24661 ccctctatat agaatgacac tgcgaccaga ctcagttatc tttttggttg ctttaagagc
    24721 tttttctcca tccagcggct tgaaaccttc agaatagacc gcaaatacat ggatttcgct
    24781 atcttgagcc agataatcca gataatctcc gacagtcagg tccatctggt tgccaatggt
    24841 tattgcatat tttgcattga tgttaggatg cttggagatg cgtgttatgg caaaggctcc
    24901 tgactgcgag atcacagcaa gcggttccac tttgcctttg ggcatgggca gcttgtattc
    24961 aggtataaag agcgtattgt atttgccagg aaccgagcgt attccaagac agttgccacc
    25021 atttatcaac ggcccttttc cttgggcgcg cgcttcggct agagcgcgat tcatctcagc
    25081 aactattgat tctgagcctt ctttttcttc tagccctccg gggattaaaa gaaccgacca
    25141 tgctttgtca tgtcgtgcaa tgtcggcaag cgtgcttgga ataccaagcg ccggaattac
    25201 catgacaaag agatcgactt tctcaggcag agattgaaca tctgcaacgc atcgacagcc
    25261 atctatccat tgaacacctg gctttatcaa atagattcta gatatatcga agccattttc
    25321 aaggagattg cggagaatta tgcgaccgtt gttgattgtc ttttcagata cgccaattat
    25381 agctacgctc gaaggctcga gaatgcgcgc tatttgatct actggccgag tctgctgggc
    25441 tagattgata ggcaagcctt cggaatcgcg tacaaggccc tggatcaggt tttgggcaga
    25501 gctttgcaca gaggcgttta tcggagctgg ggctgcaaaa tctcttagtg tggcaaggca
    25561 atccagtgcg acgagctcgc ccgtttttga aagaaccatt gggttgactt cgaattcgct
    25621 tatacctgcg gctgaaagag catctgcaga atcgataaag cgctggatgg cttcgactag
    25681 actcttttca tctaactctg gcgtcgtatt tcttaagcca gcgcataaga gtccatagat
    25741 tacattgttc tctaaaagcg attcgagcgc agagctattt gttactcttg gagagagaaa
    25801 aaggtttgcc gagccaggct tgaatttaga tgcgagatat tcggtataga taccgccagg
    25861 gccgaaactc acaataggcc cgaaatcatg cgtaaaacga tagccaaaaa tcatctcatg
    25921 gccaagcttc ggctcgaaag ctatgaactc attgacggta aaaccatctc gagacacatt
    25981 cttgaagcgc gcttccattc tgcagagcga atcggcaatt gctgccactg tgttgtcgac
    26041 aatctcgata ccttgtactt cggtcttatg aaggacctga gaacttatga tctttattac
    26101 agccttggag ccagaaaaag ggccttgtat aggggcatcc attgctccgt tagctccgat
    26161 tttctctaga aactcctttg ccgactgtac gagccaatac cgtggcgttc gtacacccat
    26221 tgcagccaga atagacattc cttcgagctc tgtaagtgct tctctgtgtt cactctgggc
    26281 tcgcgctatt attcgcctta cctcatctcg cagcactttt gccgaccgat tcagactata
    26341 gtctttagcg ccttgaactg tcgttcgact atcggtcttt tttgtttcaa ccatgataca
    26401 atgccctccc cgcttcaaaa gccttgagat tggcctgtat taccaactcg ccctttctag
    26461 agaagagctc gattatctcc ttttcgatag cattcggaga aacaggaagg agatccattg
    26521 cggcgccaat taggacgagg tttgcagttc ggacatttcc agcttgccgt gcgatgtctt
    26581 ctgcatcgat gagccgagag cgagggattt tgcggatttc tgccagcaca atctctagat
    26641 ctgggtaatt gggaatgttg aggacaggtg cagtggctga gacaatcgta ccattggaag
    26701 gcgatatcca cgaaagatga cgaagcgctt ccaggggttc gaaagcaaac acaatgtccg
    26761 ctttgccctt ggaaatcgtg ggactataga tctccttatc agcgatgcgc aaatgagcga
    26821 ggacctcgcc tccgcgctga gacattccat gtatttcgga ttgcttaacc tgaaaaccag
    26881 cagccattgc ggcacgggcc aagattatcg atgcagaaag cccaccctgg cctccaacgc
    26941 tgcagagaat tatatcgcag gtcatgattg cctcgctttc ttcagccatt cgatgcattc
    27001 ccggaacatt atgatgaccg agacaccatc gtagcaaagc tcttcgc
//
LOCUS       DAOSOB010000022        26790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1802_length_26790_cov_68.924780, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000022 DAOSOB010000000
VERSION     DAOSOB010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 26790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26790
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1802_length_26790_cov_68.924780"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLS88_03760"
     CDS             complement(<1..650)
                     /locus_tag="PLS88_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOU06498.1"
                     /translation="MSLNTKLINLLKTAPCFVDDEGELVLAAVQDHAWKLDHDLVKLL
                     LSDIEIKDKFFEEIDGYWIFNINTFIDYISQKNFLDNSYTRFRNRIGLTIGGKYLRER
                     GEVALAWPYKDCVLEGGQTKEEEKRKEIFFNEVLAQDEINRLLDPKVLTNFTRYTAKG
                     TEKVTDFKRDENGVIRENLIIKGNNLLALHTLKTQFREKVKLIYIDPPYNTGNDSFG"
     gene            complement(1327..1527)
                     /locus_tag="PLS88_03765"
     CDS             complement(1327..1527)
                     /locus_tag="PLS88_03765"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOU06499.1"
                     /translation="MIPGEVTQLAGGFIFSFWDGLALTSMRIALSSAIDFWLSRVLGS
                     RFLMAFLKIDQYEKLRKFSEDA"
     gene            1904..4015
                     /locus_tag="PLS88_03770"
     CDS             1904..4015
                     /locus_tag="PLS88_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06500.1"
                     /translation="MTEKAHTWRFRRIGGMDQVVLQTSADIEALAELDPKLWVVLAMP
                     TTQNRCSDALKLLDIDHDGKVKTAEILGAIEKLKAAFTSLDVLFSENASIKQEFIKDK
                     SLNNACKIAAQIAGSNIDEIDLDIVNKAIELFNTQLWNGDGVIVIESTDEPEVKEYIA
                     ALIAGGYCTQDSSGNMGVDLASIEAFKKEAQDFLSWIEIGEALKKELPFTDLDTALAA
                     YKSVATIVDDYFQKCALLGLTGKPDALNALMVKITEALSSAPENNREALRILPLSIPQ
                     IDGFLDIHGPFNPFYEPQARAFFGIASAAYELDSKLERESWKRIVDDMEKYETWLAAK
                     PVGRAQSIPPVRLKEMIQDSSLNKLLLLIEKDLQEAPRAEALRQLRDLLIIKRDFLKI
                     LRNFVTMDDFYATRKSIFQSGRLFLDGREFELCLDVNNPNAHPSLVSLSNMYLVYCDI
                     FRTSGEKRTIIAGITAGEADGIYIGRNGIYIDDEGANWNATITRVLIQPISIREAFFS
                     PYRWLARTIESLIVKRASTTESERQNQMKSAAEKTLEAPIEGPQVNSQAVAKKIDVGT
                     VAAIGVALGSIGAMITSILSLFIGLGVWMPLGIALVFLLISGPSMILAAIKLRKRDLS
                     PILNAEGWAVNGHLLLNIIFGSSLSHLATLPSNSVRTLNDPYAPKRKPWVLYLLILLI
                     ILAIAAWLLGWIEPIIDFFRK"
     gene            4150..4650
                     /locus_tag="PLS88_03775"
     CDS             4150..4650
                     /locus_tag="PLS88_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOU06501.1"
                     /translation="MPNDQTTTFLVDQRVVYPSQGVGRIMEIREKTFNSQKTLYYIIY
                     LEFSDMTVMVPVDKVEALGIRPLVSKEEAEVALDSISKAASTIPSDWKLRYQMNLDLL
                     KKGSIMDIAAVVRSLYQRSKIKELPILERKLYESALNLLQDEIAFALDRPKEEIAAII
                     RENLDK"
     gene            4647..5882
                     /gene="ispF"
                     /locus_tag="PLS88_03780"
     CDS             4647..5882
                     /gene="ispF"
                     /locus_tag="PLS88_03780"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOU06502.1"
                     /translation="MSLPVQKTIRVHKLPIDGSIFGLITAAGSSSRYGSSKKELEDLG
                     GLTVLERAVEPFIPFCNAILITCPKGEKSRFEELFSKSKLKPVLASLKSGFIIAEGGK
                     SRQESVLFGLRELAANCHDDGVVLIHDGARPWISKKLIEKTLNCARERGACLPLMPLT
                     ETPKIVEGDLIHDHPARGIIMSAQTPQAFSFPEILAAYELAHREGFKATDDAMIWDRY
                     VGPVSWIEGERQNRKITFKEDLVTGISSLESHSFKMPLSIGLGYDIHPLVDGRPLLLA
                     GVLIESEKGEAGYSDGDVLWHAIIDAMLGAAAIGDIGLLFPPGEPEWKNADSSVLAKK
                     AFALIRTEGWQLANIDCTVIIEKPKLAPYREAIRTSISRNLELPIESISLKAKTKEGF
                     DATGHGEAIEAHAVVLIYR"
     gene            complement(5951..6751)
                     /locus_tag="PLS88_03785"
     CDS             complement(5951..6751)
                     /locus_tag="PLS88_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06503.1"
                     /translation="MNTITAYFAALFGNFLLSLGFSLQKKHVHWLSAKRRGESIRHGE
                     IIGWVSGFILMNVQPIFNYLALEKLAPNIVAALGGSNIIFTILLSYFMLGEQIPSRKV
                     PFIFLLMASLAYAGLVGQEASQQFNAQAFWVAFFIPTAFAMLVLFASKRMSPERIGLF
                     LGSAAGALGGFMVLALEALRMNYGLSVLNWFLSAYLYVYLFCGIASFLIKQFAFERGK
                     MTAVAPSFYGLLVLYPSIAAYFVSNVPVRIPQLIAFGGISAAIVLISI"
     gene            complement(6748..7830)
                     /locus_tag="PLS88_03790"
     CDS             complement(6748..7830)
                     /locus_tag="PLS88_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06504.1"
                     /translation="MASKDSAFFAFSEGLSMREEFQRYGDHGSIFMLDFSSLDSKIDA
                     VKIRFLDISSAKWNGLGPRPSHDLKGSIELKQGLQTAAFAIDGRFLLCESSGKRIFSF
                     INGALELSLELPAAVYIGYAKNPMQACQLWHIWTMLYSHPVHWNETSLELEPQEGIGR
                     FKPRDAVGLLGSLSFSGAKPSDFQELNFSIAKDAQGYEDWKIADLMLCQPDIVLPLLF
                     RREIPMNLEVGSNRKRAQNRYAKIRAGLSQYWEFCEKRWNNEGIPAIFHPSILFPDDC
                     DYAKSDNMLFSGSDLVFSPNMASKDEFCSLILPEGKWIHLWTSRIYPSGKVTVHAPFG
                     TPALFYRLESEYAWLFDSIRQRASRL"
     gene            8188..9129
                     /locus_tag="PLS88_03795"
     CDS             8188..9129
                     /locus_tag="PLS88_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOU06505.1"
                     /translation="MKRFLVLGICLLMATSAFAQQQFITIATGGTAGTYFPLGGALAD
                     IWNKNIPKMNATAQATGASIANINLLKGGNVDVIFTQNDVASYSYNGTELLKDQAYKD
                     LRGMACLYNETIQLVALESSGIKTITDLKGKKVSVGAAGSGTEANARQILESAGLTYN
                     DIKVQYLSFGESASNMKDGNIDAAFNTAGIPTAAIQDLAVSKKMVLVPIDGQVATKLM
                     AKYPFYALQIVPAGTYQGQNQNVTTVAVKSMLAVSSKLSGDLVYKMLETMYANTDRLI
                     AAHSQGKNIKIESALEGMSIPLHSGAEKYFKEKGVLK"
     gene            9217..9723
                     /locus_tag="PLS88_03800"
     CDS             9217..9723
                     /locus_tag="PLS88_03800"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HOU06506.1"
                     /translation="MKQGRITGKKLILGCLILVVALSVIAMRLRIVPVLIIKNSQNKT
                     VARLPLYDGQFIHHYIHSIHKTAVDEYFQIKGRNLELFQVKYDTYGVGMPSDGGEAFT
                     IEDGRFVVKMHRTFSNIPIRVSIIPQHGVIVGNELLSFTQWMLPESEVTLSAGRMLIF
                     YNGRSSPK"
     gene            9720..11714
                     /locus_tag="PLS88_03805"
     CDS             9720..11714
                     /locus_tag="PLS88_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HOU06507.1"
                     /translation="MSDLPIHEQETTGDAQEILKKFDKESNYRIYSGFFAKFISALAI
                     AFSVFQLYTAIFGVLDAMLQRSIHLSFGLALIYLLYPASKKWSFNKLHPLDALLAVAG
                     ALAPMYIIINYQKLVLRAGTATTLDIVFGVIGILLVLEAARRVVGVPMVVIAVVFIIY
                     AFIGPYIPGKLAHRGANFETLVQHLYYTTEGIFGIPIGVSSTFIFLFILFGAFLERTG
                     LGQLFIDLANSVAGWAAGGPAKVAVISSALLGTVSGSSVANVVGTGSFTIPMMKRLGY
                     KPEFAGAVEATASTGGQLMPPIMGAAAFLMAEFTGIPYSRIIGAAVVPAILYYFGVWA
                     GVHFEAKKNGLRGLSREELPKLKNVITERGHLIIPLIAIIYLLVSGYTPMRAALWAIV
                     LSIISSWLKKSTRIPPIEIVRALEAGARAALGVLAATACAGIIIGVVTLTGLGLKLGS
                     VLVDIAGGKLIPTLFFTMLTSLILGMGVPTTANYVITSTITAPAVIMLLSRKAGLDPY
                     AVAPANIILPAHMFAFYFGIIADVTPPVALAAFAGAGIAKANPMKTGLNASKLAIAAF
                     LVPYIFVMNPQMLLFNINFLSFVWMLITSLIGIVAVASAVNGWLMTNTVWYERILGFV
                     GGVLLIDPGLTTDFIGIGLVAILFISQFLRSKKLKVKPSP"
     gene            complement(11832..14270)
                     /gene="thrA"
                     /locus_tag="PLS88_03810"
     CDS             complement(11832..14270)
                     /gene="thrA"
                     /locus_tag="PLS88_03810"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOU06508.1"
                     /translation="MKILKFGGTSTGSPEALKSLVSIVQKSGEKILVVSALSGTTDTL
                     LASAKLASEGGDWQSAFASIRERHYSIISALLKSQYREIATVEIKVLLDELSSLLLGI
                     SLVQDLSLRTLDLVASYGERLSATIIVQTLFQAGLNAEVVDARKLIITDNKFGAAHWL
                     ESETIQRVKSFFADFTHIAVVTGFIASTMDGVTTTLGRGGSDLTASILAACLGAEEIQ
                     IWTDVDGIMTADPRAVPDAFVIPEISYLEAMEMSHFGAKVLHPPAILPAMDRGIPVRI
                     LNTFSPDKPGTRIVKQGVPSSWPIRGIASIPSISLLLLQGPGLPGVAGIAARMFAALA
                     SAGINIILITQGSSELSICCAVLPKDAEKGALVLKEEFKYEIDSGTIQRPSIEQDLSI
                     IAVVGEMMKHRVGISGRVFKALGRNGINVVAIAQGSSELNISIVISALDRNKAMCSIH
                     DAFFLAGVRTVNVFLVGTGLIGSTLLTQVAAQHERLFRDRSIRVRIAGIANSRHMLID
                     SKGIDLENWREALQSGNKTDISAFIREILNLNLQNACFCDCTASEDITHHYETLLSAS
                     ISIVTPNKRANSGSFSNYQRLVNIARDMDVPYRYETTVGAGLPVIGTIQDLVASGDEI
                     LRIEAVLSGTISFIFNNLGKGSSFSELLAEAKLRGYTEPDPRDDLSAKDIIRKMIILA
                     REAGFSLEESQIQIESLVPKQIIHALSIEEFLALLPEMDEMMRKSCDSASRSGRVLRY
                     VAIVTPMEARLSLSSFGPDSPFYSLSGTDNMVVITSKRYSQNPLVIRGPGAGAEVTAG
                     GVFADILKTAESYL"
     gene            complement(14416..15546)
                     /locus_tag="PLS88_03815"
     CDS             complement(14416..15546)
                     /locus_tag="PLS88_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOU06509.1"
                     /translation="MEEFLSHFIASTGFIAMTWGQLIMLIVSCVLLYLGIAREFEPLL
                     LVPIAFGMLLANLPMSGITDGPKDGLVGGLIYYLYEGVRLGIYPPLIFLGIGAMTDFG
                     PLIANPWSLLLGAAAQLGIFIPFIAAYYLGFDLKACASIGIIGGADGPTAIYVTSRLK
                     PELLGPVAIAAYSYMALVPLIQPPIMRGLTTKKERLVKMAQLREVSKAERVVFPIFVT
                     ILIGLLLPAAVTLVGSLMIGNLFRETGVTDRLSDTAQHALINILTIFLGVSVGSTASA
                     ERFLNPQTLMIIALGLFAFSCGTAGGVFFGKIMYWVTGGKVNPLIGSAGVSAVPMAAR
                     VSQVVGSKEDPTNFLLMHAMGPNVAGVIGSAVAAGVFLSVLG"
     gene            complement(15564..16010)
                     /locus_tag="PLS88_03820"
     CDS             complement(15564..16010)
                     /locus_tag="PLS88_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOU06510.1"
                     /translation="MKQYRITVNGKSYDVTVEELSGVRQSTGSGFGAAPTSYSAAPAN
                     VQPVANASSPASAATASGQGSSNTANAAPANNGSVLTVKAPMPGTVIAFKVIVGQKVK
                     RGDVLLLLEAMKMENEIVAPQDGTVVALRVPASAAVNTGDPLVDLA"
     gene            complement(16033..16404)
                     /locus_tag="PLS88_03825"
     CDS             complement(16033..16404)
                     /locus_tag="PLS88_03825"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOU06511.1"
                     /translation="MTNPWMIMIVGLITVFVSLFIMIFFLLAFPRFFAVTKRREHEHP
                     APLPQMVEVTKKQAQPASSQVVEEDDALIAVLAAAVAEASGSKLGSFAITQVHQSPTE
                     SVEGFNTPIWGRVERLSRNER"
     gene            complement(16415..17959)
                     /locus_tag="PLS88_03830"
     CDS             complement(16415..17959)
                     /locus_tag="PLS88_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOU06512.1"
                     /translation="MEEKLKELEERRAKVLAGGGPKRVAAQHAKGKMTARERIEMLLD
                     PGTFVELDTFVEHRATELGMDKIDAPGEGVVTGYGMVNGRNVCVFAQDFTVMGGSLGE
                     MHAAKICKVMDLAVKIGCPLIGINDSGGARIQEGVDALSGYGEIFYRNTLASGVIPQI
                     SVIMGPCAGGAVYSPALTDFVFMTEGSSNMFITGPQVIKAVTGEEVSPEELGGAKIHS
                     EVSGVSHFSFPDEQSTLEAIRKLLSYLPQNNIEDPPIIDIGDDPTRVDDSIAELMPDS
                     PNKPYDVRDIIVRLVDAGDFFEVQPGYAKNIVTGFARLAGRSIGIIANQPKHLAGVLD
                     INASDKAARFIRFCDAFNVPLLTLSDTAGYLPGIGQEHGGVIRHGAKLLYAYSEASVP
                     KVTVILRKAYGGAYIAMCSRHLGADQVFAWPTAEIAVMGPEGAANIIFKKEIEESADP
                     EATRKEKIEEYKRSFANPYQAASRGYVDDVIDPAHTRMRLCNALNTLLGKRQNRPSRK
                     HGNIPV"
     gene            complement(18029..18433)
                     /gene="mce"
                     /locus_tag="PLS88_03835"
     CDS             complement(18029..18433)
                     /gene="mce"
                     /locus_tag="PLS88_03835"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOU06513.1"
                     /translation="MKALKIDHIGIAVEKIDTALSVWEATLGLPLNGIEEVLDQKVKT
                     AFMPIGDTEIELLESTDPEGPIGKFIATKGQGVHHLAFRVENIDKVLSELKAKGVRLI
                     DEEPRYGAGGARIAFIHPKATGGVLVELCERT"
     gene            complement(18414..19553)
                     /gene="meaB"
                     /locus_tag="PLS88_03840"
     CDS             complement(18414..19553)
                     /gene="meaB"
                     /locus_tag="PLS88_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOU06514.1"
                     /translation="MNELVGSCIKGDMRAIARLITLLENQDREAYAALYRLKDHEGVA
                     QVVGITGPPGAGKSTLVDKLISQFRARGRKVGVLAVDPTSPFSGGAILGDRLRMQSHA
                     TDAGVFIRSLATRGSLGGISKATHAAIKVLDAAGYDIILVETVGVGQSEIDIVRVADT
                     VVLISVPGLGDDIQVIKAGIMEIGDIFVVNKADRDGADRLVRELRAMLETQAQLSRGQ
                     APSGGPDTLAARDPNFMHHAGLVEGSNISAKEASTSIGRDSSTPIYFGSEQIPIPPVL
                     KTIAATGVGVAELSEAIIQHFELLKKTGELDKKRLESIKYQLGQFIFDEVSQVLNSPP
                     VSELRNNLAEQVFFRKLDVYSAGMALFHKIQLKETKGAAHESPQD"
     gene            complement(19581..19988)
                     /locus_tag="PLS88_03845"
     CDS             complement(19581..19988)
                     /locus_tag="PLS88_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOU06515.1"
                     /translation="MERKIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQTP
                     EQIVNAAIQEDVDVIALSILSGAHNHLFPRVVELLKERGVDDILVFGGGVIPEEDIPF
                     LKSKGIAEIFGPGTPTSKTIEFIRANLKRPLDR"
     gene            complement(20001..21668)
                     /locus_tag="PLS88_03850"
     CDS             complement(20001..21668)
                     /locus_tag="PLS88_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOU06516.1"
                     /translation="MTDLNKLEQDFSLWNAKVEKANEKHPERKKQFVTGSNAPINRVY
                     TPLDQKDFDYVKQLGLPGEFPFTRGVQPTMYRGRLWTMRQYAGFGTAEESNKRYKYLL
                     EQGQTGLSVAFDLPTQIGYDSDHPLSEGEVGKVGVAIDSLEDMEVLFDGIPLDKVSTS
                     MTINAPASVLLAMYIVVAEKQGVSAEKLNGTIQNDILKEYIARGTYIFPPEPSMRLIT
                     DIFEYCSKNVPNWNTISISGYHIREAGATAVQEVAFTLADGIAYVEAAIRAGLDVDEF
                     APRLSFFFNSHNDLFEEVAKFRAARRVWAYIMRERFGAKNPKSWMLRFHTQTAGSTLT
                     AQQPDNNIIRVTIQALAAVLGGTQSLHTNSRDEALALPTEEAVRIALRTQQIIAYESG
                     VAETVDPLAGSYYIESLTDRIEKEALQYLKHIDDLGGAVKAIEQGYIQKEIQDSAYQW
                     QMDVEKGERIIVGLNKFQIEEKPPKGLLRVDPAVGERQKERLSALRARRNNNMVISAL
                     ASLRNTARGTENLMFPILDAVRAYATLGEICEVLREEFGEYQPNVMF"
     gene            complement(21716..22264)
                     /locus_tag="PLS88_03855"
     CDS             complement(21716..22264)
                     /locus_tag="PLS88_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU06517.1"
                     /translation="MRVEFRLSGSGGQGLLLAGIVLAEAAIQEGMNAVQTQSYGPEAR
                     GGSSKAEVVISDSEIDYPKATEPDYLLTLSNDAYRSYGISMKKGIIIADASLNIDPPA
                     KVPVVKLPILDTASKKLGKAVVANMVALGVLGGISGVASTEALREAVRNRVPKGTEEL
                     NIAALEEGLLLAKQAKAQLGLI"
     gene            complement(22243..23109)
                     /locus_tag="PLS88_03860"
     CDS             complement(22243..23109)
                     /locus_tag="PLS88_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOU06518.1"
                     /translation="MAEFLSYFRQDRLPHIWCPGCGNGTVTGALVRAIDKLGLDKNNM
                     VIVSGIGCSSRSVGYLDFDTLHTTHGRAIAFATGVKLANPELKVLVITGDGDCAAIGG
                     NHFIHAARRNIDITAIIINNDTYGMTNGQASPMTPRGMFGTTAPYGNIEYNFDLCELA
                     KAAGATYVARATTYHYVLLEDLIAKAIMHKGFSVVEALSQCPTYFGRKNKIGGAVETL
                     NWIKDQTINIKAAASLPQEKREGKILIGELYKSEKAEFTEQYLELIGRLQKANPSYIR
                     NEGEPGCELNSD"
     gene            complement(23109..24242)
                     /locus_tag="PLS88_03865"
     CDS             complement(23109..24242)
                     /locus_tag="PLS88_03865"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOU06519.1"
                     /translation="MAETIKLMQGNEACTMAAIAAGLRFYAGYPITPSTEIAEMCSEE
                     LPKVGGKFIQMEDEIGSISACIGASLTGAKAMTATSGPGFSLMQEAIGYAGLCEIPVV
                     IIDVQRVGPSTGMPTSPAQGDVMQARWGTHGDHPIIALAPGNVKECYDLTFRAFELSE
                     KYRVPVIVLMDEVIGHMREKVVLPDPSSYAIPRRPMPESPEGYKPYAADPDGGVPPMA
                     PFGTGYRWHCTGLTHDENGAPTSKPAIAGTLARRLNLKIENKTEELADYFAEELEGCD
                     LAVVSFGSSAMASLSAVRRARKEGYKVGMLRLKTIWPFPIKTVKYLLASAKKILVPEM
                     NLGQLALEIERSAACEQPVIRLGKANGELFHPDEVFAAIKEAL"
     gene            complement(24235..24510)
                     /locus_tag="PLS88_03870"
     CDS             complement(24235..24510)
                     /locus_tag="PLS88_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU06520.1"
                     /translation="MADPQKKFFHIVRVKYCKGCNICVALCPKKVLELRNEKVFPARP
                     DDCIGCRMCELRCPDFAIEVHPVEDKSKFETSVNQVGLGLKSEASHG"
     gene            24759..26495
                     /locus_tag="PLS88_03875"
     CDS             24759..26495
                     /locus_tag="PLS88_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06521.1"
                     /translation="MKYLKIRDLTLRDGQQSQFATRMNNKQVKAVLDDYRQSHFYAME
                     VWGGAVPDSVMRFLGEDPWDRLATIKEGIGNASKLTALSRGRNLFGYNPYPDSVIEGF
                     CRNAIAAGVDIMRIFDALNDIDNMKSSIRAVKEAGGLADCAVCYTVDPRFTLSDRIKG
                     VFSGKKIPPKIFTVDYYVKKSKELASLGADMITIKDMAGLITPTMAADLIKALKNELE
                     IPIDLHTHCTPGFGVASLLAAMVNGVDIVDTAILSFSGGPAAPAYEIIRIFADRLGLS
                     VDVDNAAVARIDKSLRKIRAELAAFDQYKRLPPELDLSKDSLSPELSKLFDDAIEAAV
                     GGKYQKSLAICQQIEKAFSYPEPDEIVRHAQIPGGMYTNMLTQLKEAKLEHLLNEVLH
                     LVPTVRLDAGLPPLVTPTSQIVGVQAVNCVISLHEGKDMYENVSKNFEDLILGLYGKT
                     PWPVDPEFRYKICGTREEVPYDTSNYKPQDNPPVPEAGGTLLALNEKEQLLLELFPSV
                     ATKFLRNKRIAEWQAAHPEGTSSEAQPQLGEHPLSLYFDPNYEPSDGEPPSFWEIAPN
                     WAGWTDSIAIGQ"
     gene            complement(26514..>26790)
                     /locus_tag="PLS88_03880"
     CDS             complement(26514..>26790)
                     /locus_tag="PLS88_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOU06522.1"
                     /translation="GPRAMEESFGSVAHGAGRRMSRNAAARQIQFDTLADQMRAHSVI
                     LLAANKRLACEEAPLAYKNVDEVVETVSGVGLASLVARTKPLLVIKG"
BASE COUNT         7303 a         6365 c         5899 g         7223 t
ORIGIN      
        1 ccaaacgaat cattccccgt gttatacggt gggtcaatgt aaattagttt taccttctct
       61 cgaaactgag ttttgagtgt atggagcgcc agcaggttgt tgcccttgat aatcaggttt
      121 tcgcgtatca cgccgttctc atcgcgcttg aagtccgtaa ctttctctgt gcccttcgct
      181 gtgtaacgcg tgaagttggt caagactttt ggatcgagca ggcgattgat ttcgtcctgc
      241 gctagcactt cgttgaagaa aatctccttg cgcttttctt cttcttttgt ctgccctcct
      301 tccagcacgc aatctttata tggccaggca agtgctactt caccccgctc gcgcaagtat
      361 ttgccgccaa tagttaatcc aattctatta cgaaagcgtg tgtaggaatt atccaaaaaa
      421 ttcttttgcg atatataatc gatgaaggtg tttatgttga aaatccaata tccatcaatt
      481 tcttcaaaga atttatcctt gatttctata tcagaaagta acagtttaac caggtcatgg
      541 tcgagcttcc aggcatggtc ttgaacggcg gccaggacaa gttctccttc gtcgtccacg
      601 aagcacgggg ctgttttgag caagttgatt agtttcgtat tcaacgacat ctattactcc
      661 cttgttcaat aatctcttca tctagttaag cagcataaca tcgagtgtca actgcgccat
      721 ccttggcatt ggctgcattc tcttttcaga tagccttttt catttctggc attccgaata
      781 tcatggtcaa catccagaac tgttctttag cgattcccca ggttgacagt atgcagctac
      841 caccagccta aggcatatca acaacccgac acactgtttc atcttttgtt tcctttattc
      901 aatatatttt acagcaaaaa atcctataag aaaagaaata gtaactggaa aaagcaaagt
      961 tatggaataa gacgccaata caatctcaat acgatgacaa acttgatcat tctaattaca
     1021 agggactctt agcgctcatc aataaagaag ccgatgcctg ctgatatatg cttcgatgcg
     1081 ttcgcggaac aacatcaaca atatcatcaa caacactgtg ccgaccacaa gcgcgatcac
     1141 aattccaaag ctctaattgt atgctgcact tccggcaagc ttgttgccta gtattccttg
     1201 catgcgagct atcgtcgatg ctgcaagaaa aggaaaaaat cgttgaacgc tagcgcccac
     1261 aaaataacac aggagattct ttggaatgcc gcgaatcaca aagagaagaa taatacccgc
     1321 aaaagttcat gcgtcctctg aaaattttct cagcttctca tattgatcga tctttagaaa
     1381 agccataaga aagcgagagc ccaaaactct gctcaaccag aaatcgattg cagagcttag
     1441 cgcaattctc atgcttgtca gagcaagtcc atcccaaaag ctaaaaatga aaccaccagc
     1501 taactgtgtt acctctcccg gaatcacaaa tactatgacc tgaaaaatct gaagaatcag
     1561 gaaaacaaac catcctgctg tcccgtatga gagaatccaa tcccggagat cctcttttta
     1621 agagaaaaga agcgatagtt tgcctttcag cagaaatcct gctataagaa gcgccaaaag
     1681 caaaattgga aaagcaaact gagaaattgt cagtctcctt tttaagccaa tctttttccc
     1741 ctttctcata atgattctat attgaattga agaaacaatt ctgacttaaa aagcgaatgg
     1801 cttagaatca ctgcccttct atcccctctg ctattttccc ttctttttat cttgctcatt
     1861 tctaattaca atcagaaagg gaatcgaaac caagggaaca aagatgacag aaaaagcaca
     1921 tacttggcgt tttcggcgta taggcggaat ggatcaagtt gttttgcaga cgagtgcgga
     1981 tatcgaagct cttgcagaac tagaccctaa actctgggtt gtccttgcta tgccaacaac
     2041 tcaaaaccga tgcagcgatg cattgaaatt actcgatatc gatcacgatg gtaaagtcaa
     2101 aactgcggaa atactcggtg cgatcgaaaa attgaaggct gcttttacaa gcttggatgt
     2161 gctctttagc gaaaacgcat caataaaaca agaatttatc aaggataaat cacttaataa
     2221 tgcgtgcaaa attgcagcac aaattgcagg ctccaatatc gatgagatcg atctcgacat
     2281 tgtcaacaag gcaatagagc ttttcaatac gcagctatgg aatggcgatg gtgtcatagt
     2341 tatagaatca actgatgaac cagaagtaaa agaatatatt gctgcgctaa ttgcaggagg
     2401 ctattgcact caggacagtt ccggaaacat gggagtggat ttggcctcaa ttgaggcttt
     2461 taagaaagag gctcaagatt ttctttcatg gattgaaata ggcgaagctc tgaaaaaaga
     2521 gctccccttt acagatctcg atacagctct tgcagcttat aaatcggttg caacaattgt
     2581 cgacgattat tttcaaaaat gtgcgcttct cggccttaca ggaaaacccg atgctctcaa
     2641 tgctcttatg gtcaaaatca cagaggctct aagcagtgcg cctgaaaaca atagagaagc
     2701 ccttaggatc ctgccgctat cgattcccca aattgatgga tttctggata tccatggacc
     2761 attcaatcct ttctacgagc cacaagcaag agcgtttttc ggaatagcaa gcgcggctta
     2821 tgagctagac tcaaaactcg aaagagaaag ctggaaacga attgtagatg acatggaaaa
     2881 gtatgaaacc tggcttgccg caaaacctgt aggccgagcg caatcgattc cacctgtacg
     2941 ccttaaagag atgattcaag acagtagctt gaataaattg ctattgctca tagagaaaga
     3001 tttgcaagaa gctccgcggg cagaagcact ccgacaactt cgtgatcttc ttatcatcaa
     3061 acgagatttt ctgaaaattc ttcgcaattt tgttaccatg gacgatttct atgcaactcg
     3121 taagagcatc tttcagagcg gtagactatt cctagatggc cgtgagttcg aactctgtct
     3181 agatgtgaac aatccaaatg cgcatccttc gctagtcagc ctttctaata tgtatttggt
     3241 ctattgtgat attttccgga cttcaggaga gaaaaggacg attatagccg gcatcactgc
     3301 gggcgaagct gatggcattt atattggccg aaatggtata tacattgatg atgaaggcgc
     3361 aaattggaat gcgacgatta cgagagtatt gattcaacct atcagcattc gcgaggcttt
     3421 ttttagccct tatagatggc tcgcgcgcac tatagaaagc cttattgtca aaagagcaag
     3481 taccaccgaa tcagaacgac aaaatcaaat gaaaagcgcc gcagaaaaaa ccttggaggc
     3541 acctattgaa ggtcctcagg taaatagtca agcagtggcc aagaaaatag atgtagggac
     3601 agtcgctgcc attggcgtcg ccttgggcag catcggtgcg atgattacta gcatcttgag
     3661 tctgttcata ggcttaggcg tatggatgcc gctaggaatc gctttggttt tccttcttat
     3721 ttctggccca tcgatgatcc tagctgcaat caagctgaga aaaagagatc taagtcccat
     3781 attgaatgca gaaggttggg ctgttaatgg acatctgctt ttgaatatta tattcggcag
     3841 ttctttgagc catctggcta ctcttccatc taattcggtg agaacactca atgatcccta
     3901 tgctcccaag agaaaacctt gggtactcta tctactgatt ctgctcatca ttctcgcgat
     3961 agcagcgtgg ttgttgggct ggattgaacc gatcattgat tttttcagga aataaggtct
     4021 tgtgactcgt tatggggcac acgacaagca cgacaaaagt gtacgacaat tttgtttaaa
     4081 gatagtcagc tagcagcaca ataagggtgt tttgactatc atccgatttt ctgctatcat
     4141 aaataatcta tgcctaatga tcaaactaca acttttctag tggatcagcg tgttgtctat
     4201 ccaagccagg gtgttggacg cataatggag attcgagaaa agacattcaa tagtcaaaaa
     4261 actctttatt atattatcta tctcgaattt tcggatatga cggtcatggt gcctgtcgat
     4321 aaggtggaag cgctagggat acgcccgctt gtctccaagg aggaagctga ggtagcactc
     4381 gattcgatat cgaaagcagc gtccacgatt ccttcagatt ggaaactgcg atatcagatg
     4441 aatctcgatt tgctgaaaaa gggttcgatc atggacattg cagcagtcgt acgctcactc
     4501 tatcagcgct ccaagattaa ggaactgccc attcttgagc gcaaattgta tgaatcagca
     4561 ctaaatctgc tgcaagacga gattgccttt gcgctagatc gtcccaaaga agagatagcc
     4621 gcgattattc gcgaaaatct agataaatga gcttacctgt tcaaaaaacc atccgtgttc
     4681 acaagctgcc cattgatgga tcgatcttcg ggcttataac tgctgctgga tcgagctcgc
     4741 gttatggtag ctccaaaaag gaattggaag acctaggagg tcttactgtc ttggagcgag
     4801 ccgtcgagcc atttataccc ttttgtaatg cgattcttat tacttgccca aaaggggaaa
     4861 aatcgcggtt cgaggagctt ttttccaaat ctaaattgaa gccggttttg gcaagtctta
     4921 aatccggatt tataattgca gaaggtggta aaagcagaca agaatccgta ctatttggtc
     4981 ttagggagct tgctgcgaat tgccatgatg atggagtcgt tctcatacat gatggggctc
     5041 gcccatggat ctctaagaag ctcatagaaa aaacccttaa ctgcgctaga gaaagaggag
     5101 cttgtttgcc tcttatgccg ctgacagaaa caccaaagat tgtagaaggt gaccttattc
     5161 atgatcatcc tgcaaggggg atcataatga gcgctcagac cccacaggct ttttctttcc
     5221 ccgagattct tgcagcatac gagctagcac atcgagaagg tttcaaagcg acagacgacg
     5281 ccatgatctg ggatcgctat gttggtcctg tctcatggat tgagggagaa cgccagaacc
     5341 gcaaaatcac ttttaaggaa gatctcgtga caggtatatc ttcactggaa tcgcactctt
     5401 tcaaaatgcc tcttagtatt ggcttaggat atgatattca tcctcttgtg gacggaagac
     5461 ctttactgct tgcaggtgtt ctaatcgaaa gcgaaaaagg cgaagctggt tattctgatg
     5521 gcgatgtgct ttggcatgca attatagatg ctatgcttgg tgcagcagct attggagaca
     5581 ttggcttgct tttccctcct ggagaacccg agtggaaaaa tgctgattct tctgttcttg
     5641 caaaaaaggc ttttgcgcta attcggactg aaggatggca gctagccaac atagattgca
     5701 cggtaataat cgaaaaaccc aaactggctc catatagaga agctatcagg acttcgattt
     5761 cccgcaatct ggagcttcca atcgagtcca tctccttgaa agcaaaaaca aaggagggtt
     5821 tcgatgccac tggccacggc gaggcgatag aagcacatgc ggtagtgcta atttatcgct
     5881 agttatcgct taggcctaca aaggtataaa ggtgactttg ctgagtcttt tttcatgcca
     5941 gatagattgc ctatattgaa atcaaaacaa tcgcggcgct tattccccca aaagcgatga
     6001 gctgtgggat acgaacggga acattcgaaa caaaatatgc tgcaatagaa ggatagagca
     6061 caagtagacc atagaacgaa ggcgcaaccg cggtcatctt tccgcgctcg aacgcaaatt
     6121 gcttaattaa gaaagatgca attccacaaa ataaatatac atagagatat gccgagagaa
     6181 accaattcaa gacgctgaga ccataattca ttctcagcgc ttccagggca agaaccataa
     6241 accctcccag agctcctgcc gcgcttccaa ggaaaagacc tatcctttct ggagacatcc
     6301 tcttgcttgc aaagagaact aacatcgcaa aagctgtagg aataaaaaac gcaacccaga
     6361 atgcctgtgc gttgaactgc tgcgatgctt cttgcccaac tagacctgca taagcaagcg
     6421 acgccataag aagaaaaata aaaggaacct ttcgagaagg tatttgctcg ccgagcataa
     6481 aataggaaag caaaatcgta aagattatat tcgagccacc taatgccgcc actatattcg
     6541 gtgccaattt ctctaaagca agataattga agattggctg gacattcata agaataaaac
     6601 cgctgaccca ccctattatc tcgccatgcc ttatgctctc gccgcggcgc tttgcagaca
     6661 accaatgtac atgcttcttt tgaagagaaa agcctagaga caagagaaag tttccaaaaa
     6721 gtgcagcaaa ataagcggta atggtgttca taatctgctt gctctctgcc tgatcgaatc
     6781 aaaaagccat gcatattcgc tttcgagtct gtaaaaaagt gccggtgtac caaagggagc
     6841 atgaacggta actttgcccg aaggataaat cctggatgtc catagatgta tccatttgcc
     6901 ctcgggcaaa atcagcgagc agaattcatc tttgcttgcc atattaggcg aaaaaacaag
     6961 atcagaccct gaaaagagca tgttatcgct tttagcataa tcgcaatcat ctggaaataa
     7021 aatcgacgga tgaaaaatgg cgggtatccc ttcattattc catcgcttct cacagaattc
     7081 ccaatattga gaaaggcctg cacgaatctt cgcatatcga ttttgagcgc gtttcctatt
     7141 gctgccaacc tcgaggttca ttggaatctc tcttctaaac aatagaggaa gaacaatatc
     7201 aggctgacat agcattaaat ctgctatttt ccaatcttcg tacccttgtg catcttttgc
     7261 tatcgaaaaa ttcaattctt gaaagtcgct gggttttgct ccagagaaag agagcgaccc
     7321 caataaccca acagcatctc taggcttaaa gcgaccaatg ccttcttgag gctccaattc
     7381 caatgatgtc tcattccaat ggacaggatg gctatatagc attgtccaga tatgccaaag
     7441 ttggcaagct tgcatagggt ttttagcata gccgatatac actgctgcag gcagttcgag
     7501 cgacaattct agtgctccat ttataaacga aaagatacgc tttccagacg attcacatag
     7561 tagaaatcta ccgtcgattg caaatgcggc agtctgaagg ccctgcttta gctctatgct
     7621 ccccttcagg tcatgagatg gtcgaggacc aagcccattc catttagcgc tcgaaatatc
     7681 tagaaagcga atcttgacag cgtcgatctt tgaatctaaa gaagaaaaat caagcataaa
     7741 aatcgagcca tgatcgccat accgctgaaa ttcttctcgc attgaaaggc cttccgaaaa
     7801 ggcaaaaaat gcgctgtcct tagatgccat ggatggcatg cagctccctg cgcctctcca
     7861 tgctgaccat atttcccctg atcgatgaac aaaaatagcc ggccgcttcc tggtgtgcca
     7921 tagaagccgc tggcccgcaa tagttatgat aaaaccttgc ttccagttct caaatgaaat
     7981 caaaggtagt ctccgatatc tgctcaatgg ccctaaagcc ttcaaccttg gctgcaatac
     8041 caaaaatatc caatattatt accatataca attggagcag gatgagcaac aaccatttat
     8101 catttgcatt catgaaaaac atagtgtata atttagaaat cttagctttt atttcatgcc
     8161 aaatattttt acaggaggtg aagaagcatg aaaagattct tggtactcgg aatttgtttg
     8221 ctcatggcaa ccagcgcatt cgctcagcag caattcatta caattgcgac tggcggcact
     8281 gcgggtactt attttccact cggtggtgcg cttgctgaca tctggaataa gaatatcccc
     8341 aagatgaacg caacagccca agcaacaggc gcttctattg ctaacataaa cctgctcaaa
     8401 gggggcaatg ttgacgttat ttttactcag aatgatgtcg caagctattc ttacaatggt
     8461 actgaattgc tgaaagatca agcttataag gacttacgcg gcatggcctg tctgtataac
     8521 gaaacaattc agctcgttgc tctagaatcc agtggcatca aaaccataac cgacctgaag
     8581 gggaagaaag tatccgtagg cgctgcagga tctggaacag aagccaatgc tcgtcaaatt
     8641 cttgaatctg ctggcctaac ctacaatgat atcaaggttc agtatctgtc ttttggcgaa
     8701 tccgcgagta acatgaagga tggcaatatc gatgccgctt tcaatacagc cggaattccg
     8761 acagcagcca ttcaggatct tgctgtttct aaaaagatgg tccttgtgcc tatagatggt
     8821 caggttgcaa caaaactcat ggcaaaatat cccttctatg cgttgcaaat agtcccggct
     8881 ggcacctacc agggacaaaa tcagaatgtg accacagttg cagtcaaatc gatgctcgcc
     8941 gtgagcagca agctgagcgg agacttggta tacaaaatgc tcgaaaccat gtacgcgaat
     9001 accgacagac tgattgctgc gcattcccag ggaaagaaca tcaagatcga atctgcactt
     9061 gagggcatgt cgattcctct gcattcagga gcagaaaagt atttcaagga aaagggtgtt
     9121 ctgaaataag ctaaaagttt tggctaacgg aaactggaat gccatagacg gcgcggaact
     9181 ttttcgtttc gcgccgttta ttcaggcaaa aacaacatga aacaagggag gataactggc
     9241 aaaaaattga tcttagggtg tttgatcttg gtcgtcgccc tgagtgttat cgcaatgcgt
     9301 cttaggatcg tgccagtctt gatcataaag aacagccaaa acaagactgt tgcacgatta
     9361 cctctctatg atggacaatt catccatcat tatattcatt cgattcataa gactgcggtc
     9421 gatgaatact tccaaataaa aggaagaaat ctcgaacttt ttcaagtgaa gtacgataca
     9481 tatggcgttg gtatgccttc tgacggtggg gaggctttca ctattgaaga tggtcgtttt
     9541 gtcgtcaaga tgcaccgaac attctccaat atccccatcc gagtatcgat tattcctcaa
     9601 catggagtca tagttgggaa tgaattattg tcttttacac aatggatgct gccagaatca
     9661 gaagttaccc tctccgcagg gagaatgctg atattttaca atgggaggag ttctccaaaa
     9721 tgagtgattt accaatacat gaacaggaaa ctaccggcga tgctcaagag attttgaaga
     9781 aattcgataa agaatccaat tatagaatct attcgggttt ctttgcaaag tttatttcag
     9841 cattggcgat cgcattttct gtatttcagc tctatacagc gatttttgga gttttagatg
     9901 caatgctcca gagatcgata catctttcat tcggcctagc acttatatat ctactttatc
     9961 ctgctagcaa gaaatggtcc tttaataagc tacatccact agatgcgttg cttgcggtgg
    10021 caggagcgct cgctcctatg tacatcataa taaactacca aaagttggta ttgagagcag
    10081 gaacggctac cacgctggat atcgtttttg gcgttatagg aatattgctt gtgctagaag
    10141 cagcacgccg tgttgttggt gtgcctatgg tggtaatcgc ggttgtcttt atcatctatg
    10201 cctttattgg cccatatata ccaggcaaac tagcacaccg aggcgcaaat ttcgagactc
    10261 tggtgcaaca tttatattat acgaccgaag gtatttttgg gattccaatt ggcgtctcga
    10321 gtacctttat atttcttttt attctctttg gtgcatttct tgaaagaacc ggtcttggtc
    10381 aacttttcat cgaccttgct aattctgtgg caggctgggc agctggaggt cctgcaaagg
    10441 ttgcagttat ttcgagtgct ctattaggca cagtgtcagg atcatcagtc gcgaatgttg
    10501 taggtacagg ctcctttacc attccaatga tgaaacgcct cggatacaag cctgagtttg
    10561 ccggtgctgt ggaggcaacg gcttctacag gtggccagtt gatgccgccc ataatggggg
    10621 ctgccgcatt cttgatggca gaattcactg gtattcctta ttcccgaata attggtgcag
    10681 cggtggttcc tgcgatactc tattatttcg gagtatgggc tggtgtgcac tttgaggcca
    10741 agaaaaatgg gctcagagga ttaagtcgag aagagctgcc aaaactcaaa aatgtgatca
    10801 cggaaagagg acacctcatt atccctctca tcgcaataat ttatttacta gtatcgggat
    10861 atacaccaat gagagctgca ttatgggcta tagtgctttc cataatatcg tcctggctaa
    10921 agaaaagcac acgcattcct ccgatagaaa tcgtgcgagc cttggaggct ggagcacgtg
    10981 ctgctctagg agtactcgca gctaccgcat gcgcaggtat catcatagga gttgtcaccc
    11041 taacgggcct tggcctcaag cttggatcgg tgttggttga tatagccgga ggaaagctaa
    11101 ttccaacact attctttacg atgctcacgt cgctaatact cggaatggga gtgccgacta
    11161 ctgcaaacta tgtcatcact tctacaatta ccgcccctgc tgtcattatg cttctatcaa
    11221 gaaaagcagg cttagatcct tatgctgtgg cgcctgcgaa tattattcta cctgctcaca
    11281 tgtttgcatt ctattttggc atcattgcag atgtaacgcc tccggtcgct ctggcggcct
    11341 ttgcgggagc aggtattgca aaggccaatc caatgaagac aggcctaaat gcatcaaagc
    11401 ttgccatcgc agcctttttg gtgccataca tattcgttat gaatcctcag atgcttttgt
    11461 tcaacatcaa ctttttatct tttgtctgga tgctaataac aagccttatc ggaatcgtag
    11521 ctgtcgcttc tgcggtcaat ggatggctga tgacgaatac tgtatggtac gaaagaatac
    11581 taggatttgt aggtggagta ttgttgatag atccaggcct tacaaccgac ttcataggaa
    11641 ttggacttgt agcgatattg ttcatcagcc aatttttgcg ctctaaaaaa ttgaaagtga
    11701 agccatcacc ttgatcggtt cgatggtatc tcaatatagc agcaaaaaaa gtacgtggct
    11761 ttcaacgaaa gaggctgcca tctaaggtat ggcaaccttt ttcgttttat atattccact
    11821 atctaagttt cttagagata ggattcagcc gtcttgagga tatcggcaaa cacaccgcct
    11881 gccgtcactt ctgctcctgc gcctggacca cgtataacaa gcgggttttg agaatagcgc
    11941 tttgaagtga taaccaccat attatctgtg cctgacagac tataaaatgg gctgtcgggt
    12001 ccgaatgagg agagggaaag ccgtgcctcc attggtgtga ctattgcaac atagcgcaat
    12061 actctcccgg atcgagatgc tgaatcgcag gactttctca tcatttcatc catttctggc
    12121 aaaagcgcca agaattcctc aatagataaa gcatggatta tctgttttgg aacaagagat
    12181 tctatctgta tctgcgactc ttccaatgaa aaaccggctt cgcgcgccaa aatgatcatt
    12241 tttctgatta tatctttagc gctcaaatcg tcgcgcggat ctggctccgt atatccgcgc
    12301 agcttagctt ccgcaagaag ctcggaaaaa gaactgccct ttccaagatt gttgaaaata
    12361 aaactgatgg ttccagataa cactgcctct attcttaaaa tttcatcacc tgatgctact
    12421 aagtcttgaa ttgtaccaat gactggcagg ccagccccca ctgttgtctc gtagcgatag
    12481 ggtacatcca tatctcgtgc aatattcact aatctctgat aattgctaaa tgagccgcta
    12541 tttgctcgtt tgtttggtgt cacaatagag atcgaagctg acagcagtgt ttcatagtga
    12601 tgtgttatat cctcgcttgc tgtacagtcg cagaaacaag cattctgaag attgaggttt
    12661 agtatctctc ggataaaagc agagatatcg gtcttattac ctgattgcag tgcttctctc
    12721 cagttttcga gatcgattcc cttggaatct ataagcatat gtcgcgaatt tgcgataccc
    12781 gctattcgca cgcgaatcga ccgatcacga aaaagcctct catgctgtgc cgctacctgc
    12841 gtaagcaatg tgctaccaat caatcctgtt cccacaagaa acacattgac ggttcgcaca
    12901 cctgcaagga aaaaagcgtc gtggatggaa cacatcgcct tatttcgatc cagagcgctt
    12961 attactattg atatatttag ctcagaggat ccctgggcta tggcaacaac atttatgcca
    13021 tttctcccca gagctttgaa gacacggcct gatatcccga ctctgtgctt catcatttcg
    13081 ccgacaaccg caattatgct gagatcttgc tcgatggatg gacgctgaat ggtgcccgaa
    13141 tcgatctcat atttaaattc ttctttaaga acaagagcac ctttttcagc atccttagga
    13201 agcacagcgc agcaaatgga gagctcgctc gatccctgcg ttattagaat tatatttatg
    13261 cctgcagagg caagggcggc gaacattctc gcggcgatgc cagcgacgcc tggcaaaccc
    13321 ggtccctgta acaagagaag agatatcgat ggtattgaag caatgccacg gataggccag
    13381 ctacttggta ctccttgttt tacaattctt gtccctggct tatccgggct aaaggtattc
    13441 agtatgcgaa ctggaatacc tcgatccatt gcaggcagaa tagccggagg atgtaagact
    13501 ttcgcgccaa agtgagacat ctccatagct tctagatagg atatctccgg tataacaaat
    13561 gcatcgggaa ccgcacgagg atcggctgtc atgataccat ctacatcggt ccatatctga
    13621 atttcttcag ccccaaggca agctgctagg atgcttgcgg taaggtccga tcctcctcga
    13681 cctagggttg ttgttacccc atccattgta gaggcaataa accctgttac tacagcgata
    13741 tgcgtaaaat cagcaaaaaa tgatttaacc ctttggatcg tctccgattc tagccaatgc
    13801 gcagcgccaa acttattatc ggttataatg agctttcgtg catccacaac ctcggcgttt
    13861 aagccagctt gaaacaaggt ctgcacaatt atagttgcac tcaatctctc tccgtaggac
    13921 gcaaccagat cgagagtcct tagcgataag tcttgtacca acgaaatacc gagcagcaga
    13981 gaagaaagct cgtctagaag aacttttatc tccactgtgg cgatttctct atactgggac
    14041 ttcagaagag ccgagattat cgaataatgt cgttctctaa tggaagcaaa tgcagattgc
    14101 caatctccac cttcgcttgc cagctttgct gaggcaagaa gtgtatcggt agtaccagag
    14161 agagccgaca cgactagaat cttttcgccg gatttctgca caatgctcac gagtgacttc
    14221 agagcctcag ggctgcctgt agatgtacca ccaaatttca gaatcttcat gatccctcct
    14281 attggcggct actatatcag aataaaacga taagcgaaaa gactattaca atacgaattt
    14341 atatatattg cctaaaaact aggcgcaata aatcctacgc ctagttttct ttatccaaaa
    14401 ccagcaaggt aaatttcagc caagcacgga gaggaagact cccgctgcaa ctgccgagcc
    14461 aatgactcct gcgacatttg gacccatcgc gtgcataagt aagaaattgg tcgggtcttc
    14521 tttggatccc acgacctggc taactcttgc tgccatcggc acagccgaga cacccgcgga
    14581 acctatgaga ggattcacct ttccgccagt gacccagtac ataattttgc caaagaatac
    14641 tcctccggct gttccacagg aaaaggcaaa aagcccaagg gcaattatca tcaatgtctg
    14701 tgggttgaga aaacgctccg cactagcggt cgaaccgacc gacactccca ggaaaatcgt
    14761 gagaatattg attagcgcat gttgagcggt atcagagagt ctgtccgtca cacccgtctc
    14821 tctaaatagg ttgccaatca taagtgagcc cacgagagta acggctgcag gtagcaatag
    14881 ccctatcaag atagtgacaa atatgggaaa tacgactcgc tccgctttgg agacttcgcg
    14941 aagttgggcc atctttacca gcctttcctt tttggttgtt agtcctctca taataggagg
    15001 ctggattagc ggcacaagtg ccatatacga gtaggcagca atagcgaccg gacccaagag
    15061 ctcgggtttt agacgcgaag taacatagat tgcggttggg ccatccgcgc caccgatgat
    15121 tcctatgcta gcgcaggctt tgaggtcaaa tcctaaatag taagcggcaa taaagggaat
    15181 gaaaataccc agctgagccg cagcgccgag aagaaggctc cagggatttg caattagagg
    15241 cccgaaatcc gtcatcgcgc ctataccaag aaaaataagc ggcggatata ttccaagcct
    15301 tacgccttcg tacaaataat agataagacc accaacaaga ccatccttag gcccatcggt
    15361 aattccgctc attggaagat tggctaaaag cataccaaac gcgataggca cgagcaggag
    15421 tggctcgaat tctcgcgcaa tgccaagata tagaagaaca caggacacaa tgagcattat
    15481 tagctgcccc caggtcattg ctataaaacc tgttgaagca ataaaatgag acaagaactc
    15541 ttccatctag gaatccccct agtctatgct agatcaacga gcggatcgcc tgtgttcact
    15601 gcagcgcttg caggaacgcg gagcgcaacg accgtaccat cctgcggagc aacaatttca
    15661 ttctccattt tcatagcctc taagaggaga agaacatcgc cacgctttac tttttggcca
    15721 actattactt tgaatgcaat aacagttcca ggcattggag cttttaccgt caggacagag
    15781 ccgttgtttg cgggcgctgc attagctgta ttggaagaac cctgtccaga ggcagttgca
    15841 gcactcgcgg gcgaactcgc atttgctaca ggctgaacat tagctggtgc tgcggaataa
    15901 gaagtagggg ctgcgccaaa tccgcttcct gtagactgtc gtactccact gagctcctcg
    15961 actgttacat cataggactt gccatttact gtaattctat actgtttcat aatataattc
    16021 tccttagcta ttctagcgtt cattgcgaga aaggcgctcc actctacccc atattggggt
    16081 gttgaagcct tcgacgctct cagttggtga ctgatgcacc tgcgtaattg caaaactacc
    16141 gagtttcgaa ccacttgctt cggctactgc cgctgcaaga acagcgatca atgcatcatc
    16201 ttcctctacg acttggctgc tagctggttg agcttgtttt tttgtaactt cgaccatctg
    16261 cggcaatggt gcggggtgtt catgttctct tcgctttgtg actgcaaaga agcgcggaaa
    16321 agctagcaga aaaaaaatca tgatgaatag cgatacaaag accgttataa gtccgacaat
    16381 cattatcatc catgggtttg tcatgatagg cctctcaaac aggaatattc ccatgcttgc
    16441 gagaaggccg attctgtctc ttcccaagca gagtattcaa tgcattgcat agacgcatcc
    16501 ttgtatgagc tggatcgatg acatcgtcga catatcctcg cgatgctgct tgatagggat
    16561 tcgcaaagct gcgtttatat tcctctatct tctccttacg ggttgcctct gggtcagcag
    16621 attcttcgat ttccttctta aaaatgatat tcgctgcgcc ctcagggccc attacggcaa
    16681 tttcagccgt cggccaagca aagacttgat cggcaccgag atgccgcgag cacatggcta
    16741 tataagctcc accgtaggcc ttgcgcagaa tcacggtcac cttaggcaca gatgcttctg
    16801 agtaagcgta gagaagcttt gctccatgcc taatgacacc gccatgctcc tgccctatgc
    16861 ctggaagata accagcagta tcggacagag taagaagcgg cacattgaat gcgtcgcaga
    16921 atcggatgaa gcgtgcggcc ttatcggagg cattgatgtc gagcacacca gctagatgct
    16981 tgggctgatt agctattatg ccgatggaac ggccagcaag gcgcgcaaag cctgttacta
    17041 tattctttgc gtaaccaggc tgcacctcaa aaaagtctcc agcgtccacc agtcgaacga
    17101 taatgtctcg aacgtcatag ggcttgtttg gcgaatccgg catgagctct gcaattgaat
    17161 catctacacg agtcggatcg tctccgatat ctataatggg agggtcttct atgttgttct
    17221 gtggaagata ggacagtagc tttctgattg cctccaaggt cgattgttca tcgggaaagc
    17281 tgaaatgcga tacgcccgaa acttctgagt ggattttggc gcctccaagt tcttccggac
    17341 ttacttcttc gccggttact gcttttataa cctgagggcc agttatgaac atgttgctcg
    17401 atccttctgt cataaagaca aaatccgtca aggccggcga atagacagca cctccagcac
    17461 aaggccccat aatcacggag atctgaggga tgactccaga tgccaacgta ttgcgataaa
    17521 aaatctcacc atagcccgag agtgcatcta caccttcttg tattctcgcc ccgccagagt
    17581 cgtttattcc tatcaatgga caaccaattt tgactgcgag atccatgacc ttgcatattt
    17641 ttgccgcatg catctcacca aggcttccgc ccatcaccgt aaaatcctgg gcaaaaacac
    17701 agacatttct gccattgacc attccatagc ctgtaacgac tccttcacca ggagcatcga
    17761 tcttgtccat accgagctcg gtagctcggt gttcgacaaa ggtgtcgagt tccacgaagg
    17821 tgcctgggtc tagcagcatt tcgatcctct cgcgagcagt catcttgcct tttgcatgtt
    17881 gggcagcaac ccttttgggg ccaccgccag caagcacctt tgctcggcgc tcctctagtt
    17941 ccttcaactt ttcttccata ccgaccatcc ttgttgtaat gcctccaggg aggaggctgc
    18001 tttctacgat agttatttag agcgaattct acgttcgttc gcaaagttcg acaagcacac
    18061 cacccgtcgc tttagggtga ataaaagcta tacgggctcc tcctgcaccg tatctgggtt
    18121 cctcatcgat tagccttacg ccttttgcct tgagctcaga taaaacctta tcgatatttt
    18181 cgactcggaa ggccagatga tggactcctt gccctttggt ggcgatgaac ttgccgatcg
    18241 gaccttctgg atcggtagat tcaagaagtt cgatctccgt gtctcctatt ggcataaagg
    18301 cggtcttaac cttttggtcc aatacttctt cgataccatt cagtggaaga cctagcgtgg
    18361 cttcccatac cgagagggct gtatcgattt tctcgaccgc tatgccgata tgatcaatct
    18421 tgagggcttt catgtgctgc tccttttgtt tcctttagct gaatcttatg aaaaagcgcc
    18481 attccggcgc tgtagacatc cagttttcga aagaatacct gttctgccaa attgttgcga
    18541 agctctgaga ccggagggct gttcaacact tgacttactt catcgaaaat gaattgtcct
    18601 agctggtatt ttatagactc cagacgcttt ttatcgagct cgccagtttt ctttagtaac
    18661 tcgaagtgct gaattatagc ttcagataat tcggctacac caactcctgt tgcagcaatg
    18721 gtttttagca ctggaggaat gggaatctgt tccgagccaa aataaattgg cgtactagaa
    18781 tctctcccga tggaggtact tgcctctttg gcgctaatat tcgacccttc cacaagtcca
    18841 gcatgatgca taaaattagg atctcgcgca gccagcgtgt ctggacctcc ggaaggcgct
    18901 tgcccacgac taagctgagc ttgtgtttcg agcatagccc tgagctcgcg cacgagccga
    18961 tctgctccat ctcgatccgc cttgttgacg acgaagatgt cgccaatctc cataatgcct
    19021 gcctttatta cttggatatc gtctccaagg cccggcactg agatgaggac aacagtgtca
    19081 gcaactctca ctatatctat ctccgactgc cctacgccga cagtctcgac aagaatgatg
    19141 tcatagcccg ctgcatcgag aaccttaatt gcagcgtgag tggccttaga gatgccgcct
    19201 agactgcctc gagtcgcgag gctccgaata aaaacacctg catcggtagc atggctttgc
    19261 atcctaagcc tatcgcccaa aatggctcca ccagaaaaag ggctcgtagg atcgacggct
    19321 agaacaccca ccttcctgcc tctagctcta aactgcgaaa tcagtttatc gactaaagtg
    19381 cttttgccag cgcctggtgg gcctgttatt ccaacgacct gtgcaacgcc ttcgtggtct
    19441 tttaagcgat agagggcagc ataggcttct cggtcctgat tctccaaaag agtaataagc
    19501 cgagctatcg cacgcatatc gccttttatg cagctgccca caagttcatt catgaataga
    19561 gaactccatt ttgatactat ctatcgatct agcggccgct tcaaattcgc tctgatgaat
    19621 tctattgttt tcgacgttgg tgttccagga ccaaaaatct cggcaattcc ctttgatttc
    19681 aaaaagggaa tatcttcttc tggaattact cccccaccaa aaaccagaat atcgtccacg
    19741 cctctttctt ttaaaagctc aaccactcgc ggaaaaaggt gattgtgtgc tccagaaaga
    19801 atagaaagtg caattacatc gacatcttcc tggatagccg cgttgactat ctgctcggga
    19861 gtctgacgca gacctgtata tatcacttcc atgccagcat cgcgcagagc acgggcaatg
    19921 acctttgcac ctctatcatg tccatcgagg cctggcttag ctaccaatac tcttattttt
    19981 ctttccatgt tcgcccccct ttagaacatc acattaggct gatattcgcc aaattcctcg
    20041 cggagcactt cacatatctc tccgagggtt gcataggcac gcacagcatc aagtattggg
    20101 aacatgaggt tttctgttcc cctagctgtg ttcctcaatg aagcaagggc agaaatcacc
    20161 atattattgt tccggcgcgc tctgagtgcg gagagtctct ctttttggcg ctctccgact
    20221 gctggatcca cgcgcaaaag acccttgggt ggtttttctt cgatctggaa tttgttgagt
    20281 cctacgatga tacgctcgcc cttttcgaca tccatttgcc actgatatgc agaatcctgg
    20341 atctccttct gaatgtatcc ctgctcgatg gcctttactg cccctcccaa atcatctatg
    20401 tgcttaaggt attgtagggc ttccttttct atgcgatctg tgaggctctc tatataatat
    20461 gagccagcca gcggatctac ggtctctgcg acgccggact cgtatgcgat tatctgttgg
    20521 gtacgaaggg ctattcgtac ggcttcttcg gtaggaaggg cgagcgcttc gtcacgggaa
    20581 ttggtatgga gggattgcgt accgcccagc acggcggcca gggcttgaat agttacgcgt
    20641 ataatgttgt tgtcgggctg ctgggcggtg agggtggagc cagcggtttg ggtatggaag
    20701 cgaagcatcc agctctttgg attcttagcg ccaaaacgct cacgcatgat gtaagcccaa
    20761 accctgcgag ccgcacgaaa tttggctact tcctcgaaaa gatcattgtg agaattgaaa
    20821 aagaaagata agcgaggcgc aaattcgtcg acatcgagac ctgctcttat agcagcttcg
    20881 acataagcaa taccatccgc cagggtaaat gccacctcct gaacggcggt tgcgcccgct
    20941 tctcgaatgt ggtagccaga aatagaaata gtattccagt ttggcacatt tttagaacaa
    21001 tattcgaata tatctgtgat gagcctcatg gaaggctctg gcgggaaaat ataggtacct
    21061 cgggcaatgt attccttcaa gatatcgttc tgaatcgtgc catttagctt ctcagcacta
    21121 actccttgct tctcggctac gacaatatac attgccagaa gtacagaagc aggagcattt
    21181 atagtcatgc ttgtggagac cttatcgagt gggatgccat cgaacaatac ttccatgtcc
    21241 tctaatgaat cgattgccac acctactttc cccacttctc cttccgatag ggggtgatct
    21301 gaatcatatc caatctgggt aggcagatcg aaagccacgg aaagccctgt ttggccctgc
    21361 tcgagcaaat atttgtagcg tttattggat tcttctgcgg taccaaaacc tgcatactgc
    21421 cgcatggtcc atagcctacc tcgatacatc gtaggttgaa ctcctcgcgt aaaggggaat
    21481 tcgcccggca atcctagctg cttaacataa tcgaagtctt tttgatcaag cggagtatat
    21541 actctattga ttggagcatt cgatcccgtt acaaattgct tcttacgctc aggatgcttt
    21601 tcattcgcct tttcaacctt tgcgttccat aaactgaaat cctgttcaag tttattcaag
    21661 tcagtcatgc ttccctcctc gaggtcacca atctatagca gacggatttc atatattaga
    21721 tgagtccaag ttgagctttt gcttgcttgg ctaataggag cccttcttcg agcgccgcga
    21781 tatttagctc ttcagttcct tttggtaccc tattcctcac ggcttctctt agtgcttctg
    21841 tacttgctac tccagatatg ccacccaata ctcctagtgc aaccatattg gctacaactg
    21901 cttttcctag tttttttgat gcagtatcaa gtatgggcag ctttacgact ggcaccttag
    21961 ccggtggatc gatgttcagg ctcgcatctg caatgatgat cccttttttc attgaaatac
    22021 catagcttct atatgcatcg ttggatagcg taagaaggta atcgggctca gtggctttag
    22081 gataatctat ttcgctgtcc gagatgacaa cctctgcctt gctcgaacct ccacgcgctt
    22141 ccggtccata actctgtgtt tggactgcat tcataccttc ttgaattgcg gcctcagcca
    22201 atacgatgcc cgcaagcaaa agcccctgtc cacctgagcc gctcagtctg aattcaactc
    22261 gcatcctggt tctccttcgt ttcggatata gctgggattg gccttttgta gtctgccgat
    22321 cagttctaag tattgctctg tgaactcagc cttttcgctc ttatagagct caccaattaa
    22381 gattttgcct tcgcgtttct cctgtggcaa tgaggctgca gcttttatat ttattgtctg
    22441 atctttgatc caattgagcg tttctacagc accaccaatc ttattcttcc ggccaaagta
    22501 ggttgggcat tgtgacaagg cctcaaccac tgagaagcct ttatgcataa ttgctttggc
    22561 aattaggtct tcgagcagga catagtgata tgtagtcgct cttgcgacat aagtagcacc
    22621 tgccgcttta gctagctcgc acagatcaaa attatattcg atgtttccat aaggagcggt
    22681 tgttccgaac atcccacggg gcgtcattgg agaggcttgg ccatttgtca tgccatatgt
    22741 atcgttattt ataattattg ctgttatatc gatatttcta cgggcagcat ggataaaatg
    22801 gttacctcca attgcagcgc aatctccatc acctgttatt accaatactt taagttcagg
    22861 attagctagc tttacacccg tagcaaaggc aatagcccga ccatgcgtcg tatgaagtgt
    22921 atcgaaatcg agataaccaa ccgagcggct tgagcaacca ataccggata cgataaccat
    22981 gttgttttta tcaagcccaa gtttatcaat cgcgcgcaca agggcaccag taacagtgcc
    23041 attcccgcat cctgggcacc aaatatgggg aagtcgatct tgacggaagt aagagagaaa
    23101 ttcagccatt agagtgcctc cttgatcgct gcaaagactt catctggatg aaaaagctct
    23161 ccattagctt taccgagcct tatcacgggt tgttcgcacg cagccgatct ttctatctca
    23221 agcgcaagct gaccaagatt catctcgggc acaagaattt ttttagcgga cgctaataaa
    23281 tactttacgg tcttaatggg gaaaggccat atagtcttga gcctaagcat gcctacttta
    23341 tagccttctt ttcgagcacg cctaactgca gaaagagatg ccattgcaga agatccgaag
    23401 gatactacag ccaaatcgca accctctagc tcctctgcga aatagtctgc caactcttcg
    23461 gttttgtttt cgatctttag atttagacgg cgcgcaagtg ttcctgctat agcaggctta
    23521 ctagtaggag cgccattttc atcgtgagtc agacctgtac agtgccaacg ataaccagtc
    23581 ccaaaaggag ccatgggcgg aacacctccg tccggatcag ctgcatatgg cttatagcct
    23641 tctgggctct ctggcattgg ccttcgcgga atcgcatagc ttgacggatc gggtagaaca
    23701 actttttctc gcatatgccc aatcacttca tccatcaaga ctatgactgg aacacgatac
    23761 ttctcagata gctcaaaagc gcggaatgtt aagtcataac actcctttac attgccagga
    23821 gctaatgcaa ttatcggatg atctccgtgt gtgccccatc tggcctgcat tacatcaccc
    23881 tgagctgggc ttgttggcat accggtggaa ggaccaacac gctgtacatc gattataact
    23941 acaggaatct cgcaaaggcc tgcatagcct attgcttcct gcatcaatga aaagccagga
    24001 cctgaagtag cggtcatggc tttagcgcca gtcaatgaag caccaatgca cgctgagatt
    24061 gagccaatct catcttccat ttgaatgaac ttgcctccca cttttggaag ctcttccgag
    24121 cacatctcgg ctatctctgt ggaaggagtg ataggataac cagcatagaa tcgtagcccc
    24181 gcagctatag cagccatggt gcatgcctcg tttccttgca taagctttat agtttcagcc
    24241 atgggatgcc tccgatttta agcccagtcc aacctgattg acgcttgttt cgaattttga
    24301 tttatcttcg acagggtgaa cttcaatcgc aaaatcggga cagcggagtt cacacattct
    24361 gcatccgatg caatcatctg gtctagctgg aaaaaccttt tcgtttctta gctccaatac
    24421 cttctttggg cagagagcca cacagatgtt gcaacctttg cagtatttta ctctgacgat
    24481 gtgaaaaaat ttcttttgag gatcagccat ttctttgctc ttgaacaata atcgccgtac
    24541 tcaatctaac agaccgagca ttgctccttt agatgttcct tcaaaaagaa aaaatttaaa
    24601 ggataaatat aagcaggaac gcattacggc gttgttttat tttacagttt attgccgatt
    24661 ttgtcaaaat ctatcaattc ttgcataaac catatatgct gccgatactt gtcgcttcca
    24721 gcgatttgag atacaaatag acttcaggag tttttaagat gaaatactta aaaattcgcg
    24781 atttaacact tcgcgatggg cagcagtcac aatttgcaac acgcatgaat aataagcagg
    24841 tcaaggccgt gctggatgat tatcgccaga gtcattttta tgctatggag gtatggggtg
    24901 gcgctgttcc tgattcagta atgagatttc ttggtgaaga tccatgggat agactcgcta
    24961 caatcaagga aggaattggc aatgcctcaa aactgacagc cctatcccgt gggcgcaacc
    25021 tatttggata taacccctat cccgattctg taatcgaagg attttgcagg aatgctattg
    25081 cagctggcgt agatatcatg agaatattcg atgccctcaa cgacatcgac aatatgaagt
    25141 cttcgatccg cgctgtaaag gaggcaggtg gcctagcaga ttgcgctgtc tgctatactg
    25201 tcgatccgcg ttttacattg agcgacagaa taaaaggggt tttttctggc aagaaaatac
    25261 cgccaaagat tttcacagta gattattatg tcaagaaatc aaaagagctt gcttctctcg
    25321 gcgccgatat gatcacaata aaagatatgg caggactgat aacccctacc atggctgctg
    25381 atctcataaa agcgctaaaa aatgaacttg agattcctat tgatcttcat actcattgca
    25441 cgccaggctt cggagttgca agtctgcttg cagctatggt caatggagtc gacattgtag
    25501 atacggcaat tctgtctttc tctggaggac ccgctgctcc ggcttatgaa ataatacgta
    25561 tttttgcaga caggttgggc ctctccgtag atgtcgacaa tgctgctgtc gcaagaatag
    25621 acaagagtct aagaaaaatc agagccgaac ttgcggcttt cgatcagtac aagagacttc
    25681 cgcccgaact cgatctctct aaggatagtc tctcgccaga gctctctaag cttttcgatg
    25741 acgctataga agcagccgta ggaggtaagt accaaaaatc tcttgcaatt tgccagcaaa
    25801 tagaaaaggc gttttcctac cccgagcccg atgagatcgt gcggcacgca cagattcctg
    25861 gaggcatgta taccaacatg cttactcagc tcaaggaagc caagctggaa catctcctca
    25921 atgaagttct tcatcttgtg ccaactgtgc ggctcgatgc tggactccct ccgcttgtaa
    25981 ctcctaccag tcagattgta ggtgtccagg cggtgaactg cgttataagc ttgcacgagg
    26041 gaaaggacat gtacgaaaac gtctcgaaaa acttcgaaga ccttatcctc ggtttatatg
    26101 gaaagacgcc atggccagtc gatcctgaat tccgctataa gatatgtggc acaagagaag
    26161 aagttcctta tgacacaagc aactacaagc ctcaagataa cccaccggtt ccagaagcag
    26221 gtggaactct gcttgcattg aatgaaaaag aacaactact tctagagctt ttcccctcgg
    26281 tggcaaccaa attcttgaga aacaaaagga ttgcggaatg gcaggcagca catccagaag
    26341 gcacctcttc tgaagcacaa cctcagcttg gagaacatcc tctttcccta tactttgatc
    26401 ccaattacga accaagcgat ggagagccac catctttctg ggagattgca ccaaactggg
    26461 caggatggac agattctatc gccatagggc aatagaaagc gctatgtgct cacctagccc
    26521 ttgattacca agagaggctt agtgcgggcc actaggcttg caaggccaac accgctcact
    26581 gtttccacaa cctcatccac attcttatag gcaagaggag cttcttcaca ggctaatcgc
    26641 ttattagctg caagcagtat tacagaatga gctcgcattt ggtctgcgag tgtatcgaat
    26701 tggatctgtc gggcagcagc attgcggctc atgcgccgac ctgccccgtg tgcaacgctt
    26761 ccaaagctct cttccatggc tctaggcccg
//
LOCUS       DAOSOB010000023        26479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1835_length_26479_cov_83.237057, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000023 DAOSOB010000000
VERSION     DAOSOB010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 26479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26479
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1835_length_26479_cov_83.237057"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..4612)
                     /gene="rpoC"
                     /locus_tag="PLS88_03885"
     CDS             complement(263..4612)
                     /gene="rpoC"
                     /locus_tag="PLS88_03885"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOU06523.1"
                     /translation="MRDIQDFDNISIHLASPEMIRAWSYGEVKKPETINYRTLRPERD
                     GLFCERIFGTTKEWECYCGKFKSIRYKGVICDRCGVEVTNSKVRRERMGHIELASPVS
                     HIWFYRSVPSRMGLLLNLPMATLKSVLYYEKYIVIDPGETELKKMQLLTDEEYREAMD
                     RYGGAFTAGMGAEAIRSLLEQIDLDKMSQELRQKMIERGSKSDKRLLKRIELVEAFRS
                     SGNRPEWMILTVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKKLLQLNA
                     PDIIIFNEKRMLQEAVDALFDNTRRRRAVKGAGNRPLKSLADMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKMWQCGLPTKMALELFKPFIMKKLVEKDVVYNIKKAKMLVES
                     ETPEVFAVLDEVVKEHPVLLNRAPTLHRLGIQAFEPLLVEGKAIKLHPLVCKAYNADF
                     DGDQMAVHIPLTHAAQAECWTLMLSSRNLLDPASGKTIVYPSQDMVLGINFLSRHRPG
                     VKGEGKRFSSTDELLLACEYGSCDYQALVYVAAPKEPVWNSYEKEAELSGNHFIKTTG
                     GRILLNEALPKEVPFINYAMTDKDIRMLIEHVYRTTSPFITVNMLDTIKEMGFKYATF
                     FGATIGMDDIVIPKEKAEMIEKANKEVEAIQQQYLAGHITQDERYNRVVEVWSKTNEE
                     LTAVMMKTLEKDRDGFNNIYMMANSGARGSKNQIRQLAGMRGLMAKPSGDIIELPIRS
                     NFREGLSVIEFFISTNGARKGLADTALKTADAGYLTRRLVDIAQDVVVNEDDCGTING
                     SIYTALKDGDEIIETLAERIIGRFTLDPIRHPITGQIIVGTNEEITEEIAEEIEEAGI
                     GEVSVRTVLTCEAEHGVCRRCYGRNLATGRTVDIGEAVGIIAAQSIGQPGTQLTMRTF
                     HIGGAATKAAEENRISLHYPVYIADIEGSCVKTELGRLLFTRRGYIYVNRVFGVFDFT
                     DEDTLLVKDGQRVLKDDSVIKKADGSYIKSPSISYAKVIDNRLLLISQEQKVEIRNGS
                     EMVVSRGQILLANETIATFDPFNDPIISEHDGIVRYEDIMPGATLKEEINAETGNVEK
                     IITDVGNERDTKEPRIIITDEAGNDIETYYLPEGAYVNVEDGETVRAGKMLAKILKES
                     AKAMDITGGLPRVGELFEARKPKSPAVLALVSGTVTFGGTLKGKRVINIRDSFGKTHK
                     HLVPKNRRLLVRNGDKVVAGEMLCDGVKNPHDILAILGEQECQRYLMDEVQQVYRAQG
                     VTINDKHIGVIIRQMMKKVEIVNPGDTVFIYGQQVDKHKFHDENRRVMEEGGQAAVAR
                     PILLGITRASLKIDSFFAAASFQETTRVLTDAAIKGEIDQLRGLKENVIIGHLIPAGT
                     GMKQYRQLKLFDAENDDLNALVEEVLEKRKQERELAPPAILQDRTSDISFEETTIFDN
                     DEGFSEDNESLEE"
     gene            complement(4748..8242)
                     /gene="rpoB"
                     /locus_tag="PLS88_03890"
     CDS             complement(4748..8242)
                     /gene="rpoB"
                     /locus_tag="PLS88_03890"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOU06524.1"
                     /translation="MTYSGQKIKRTYIGKEFHDVMDIPDLIDIQLSSYEHFLQRERLR
                     KGEPLEPIGLEEVFRTTFPIESPSGDLVIEYERYSLDEKAIKYSELECKQKTLTYSVP
                     LKAVINLVFKKTGEIRQKEIYLGDIPLMTDRGTFIINGAERVIVSQIHRSPGVIFSHD
                     KGVFSSRIIPYRGSWLEFEIDQKHDLIHAKIDRKRKILGTMFLRAIGFNSREEIIAAF
                     YTTKTVQLSEDQNDKDALVGKMLAKPVYMEIDGEQKRLFRAGDKIHLHEIDELINLGV
                     DSIEIIDFEDDNSLHNEMILNCFDREDVKLVKETPDQDEPTKEDAISAVYSVLRPGEP
                     ISLENAERDLHNMFFSARQYDLGDVGRYKLNKKFDFDIKTNTLTKEDIVATMKHLINI
                     YYGEANIDDIDHLGNRRVRAVGELLANVMKVAFSRMERTAKDRMTGKDLETAKPQDLI
                     TIKPIVAAIKEFFGTSQLSQFMDQVNPLAELTHKRRLNALGPGGLNRERAGFEVRDVH
                     YTHYGRMCPIETPEGPNIGLIVSLATYTTVNEYGFLEAPYRRVRNGIVTDEIEYLSAI
                     DEDKYYIAQASAHIDKSGRFLDQNISCRHQGDYTMRSPSEIQFMDVSPKQIISVSAAL
                     IPFLEHDDANRALMGSNMQRQAVPLVFTEPPRIGTGMEWKTAYDSGVLVKAKRSGTVE
                     CVDANHIVIRPEKPDSPDDKDVYQLIKYQRTNQDTCFNHRPIVKHGEKVLKGQVIADG
                     PATNQGELALGRNILAGFVPWNGYNYEDAILISERVVKEDLFTSIHIKEFILEVRDTK
                     LGPERITRDIPNTSEKMLDNLDGQGVIRIGAKVKSGDILVGKVTPKSDSDTTPEFKLL
                     NSIFGEKAKDVRDSSLHVPHGIEGTVIDVQRMSRANNDDLQPGVEETVKVLVAVKRKL
                     KEGDKMAGRHGNKGVVARVLPVEDMPYLDNGTPLDICLNPLGVPSRMNIGQIMETELG
                     WAASTLNEWYECHVFQSPSQEQIEEKLREAGLPATSKVMVRDGKTGEFFKNPITVGVV
                     YFLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGAANTLQ
                     ELLTIKSDDMTGRSKIYEAIVKGEPHTAAGIPEAFNVMVQELRGLALDIKVYDSKGKQ
                     IALTERDEDIIKKQTGAF"
     gene            complement(8447..8833)
                     /gene="rplL"
                     /locus_tag="PLS88_03895"
     CDS             complement(8447..8833)
                     /gene="rplL"
                     /locus_tag="PLS88_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOU06525.1"
                     /translation="MAALTKEQIIDAIASMTVLEISELVKAMEEKFGVTAAAPVAVAA
                     PAASAAAAPVEEKTEFTVILKGNVPADKKIAIIKEVRSITGLGLKEAKDLVEAGDKPL
                     KENISKEEADKIKKQIEAAGGTVEIK"
     gene            complement(8962..9513)
                     /gene="rplJ"
                     /locus_tag="PLS88_03900"
     CDS             complement(8962..9513)
                     /gene="rplJ"
                     /locus_tag="PLS88_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOU06526.1"
                     /translation="MAIKASKIQPRKVEAIGMLREMISGSNDFIFTEYRGLTVEQITT
                     LRQQLREKGVELHVVKNNFARLAFEELGYSHAVELVLKGPTAVAFIKTDSNEVAKILL
                     DFAKETPALIIKGAMIDKEFMDADKVEAFSKLPGRNQLIAMLMSTMQAPAQNLVYVIN
                     AIPTKLVRVLKAIEEKKAKEEAL"
     gene            complement(9518..10204)
                     /gene="rplA"
                     /locus_tag="PLS88_03905"
     CDS             complement(9518..10204)
                     /gene="rplA"
                     /locus_tag="PLS88_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOU06527.1"
                     /translation="MKRGKKYIEVAKKVDHSQAYDIGDACALVKELKTAKFDETVEAH
                     IRLNLKKSQSVRDTVVLPHQFRAEKRILVFCKPERAKEALEAGATYAGSDEYIEKIKG
                     GWLDFDIAVATPDMMKDVGKLGMVLGRRGLMPNPKTGTVTNDLSSALSELRKGRTEYR
                     TDKSGIIHLPVGKVSMEAEKIAENLSTLVEEVNRKRPSDVKGDFVASVFLTPTMGPSV
                     KVALNKSEKR"
     gene            complement(10206..10637)
                     /gene="rplK"
                     /locus_tag="PLS88_03910"
     CDS             complement(10206..10637)
                     /gene="rplK"
                     /locus_tag="PLS88_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOU06528.1"
                     /translation="MAKKKITAIVKLQCPAGKATPAPPVGPALGPHGVSAPMFCQQFN
                     ERTKNMEPGLVIPAIITIYQDKTFTFVLKTPPASVLIKKACGIEKGSSFPQKDKVAKL
                     SKAKLEEIAKQKLPDLNANDLEAAKRIVAGTARSMGVDVEL"
     gene            complement(10807..11364)
                     /gene="nusG"
                     /locus_tag="PLS88_03915"
     CDS             complement(10807..11364)
                     /gene="nusG"
                     /locus_tag="PLS88_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOU06529.1"
                     /translation="MAKSWYILHVYSGYENKIEKTIRMLIDSGELSRDIVTDVKVPTE
                     EISELKEGKKRAVSRKILPGYILVEMDLPEDGWKATCATIRNINGVTGFVGSSSNARP
                     QPITMEEVKRIFQRTGELKGERPVYFRQSFSVGEQVKITQGPFESFSGVIDEVNTEKN
                     KIKVSVQIFGRSTPVEVDMAQVEKI"
     gene            complement(11377..11559)
                     /gene="secE"
                     /locus_tag="PLS88_03920"
     CDS             complement(11377..11559)
                     /gene="secE"
                     /locus_tag="PLS88_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOU06530.1"
                     /translation="MKKIIQFFKDSYAELSKVVWPSKDEVIASTKIVIISTVAVALVL
                     GLIDFLIVLGIEVVFR"
     gene            complement(11629..11701)
                     /locus_tag="PLS88_03925"
     tRNA            complement(11629..11701)
                     /locus_tag="PLS88_03925"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11666..11668),aa:Trp,seq:cca)
     gene            complement(11710..11886)
                     /gene="rpmG"
                     /locus_tag="PLS88_03930"
     CDS             complement(11710..11886)
                     /gene="rpmG"
                     /locus_tag="PLS88_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOU06531.1"
                     /translation="MASKKKQNAEIIALQCTECKRRNYTTKKNRKTTQEKLELMKYCK
                     WDHKHTLHKETKVK"
     gene            complement(11951..12023)
                     /locus_tag="PLS88_03935"
     tRNA            complement(11951..12023)
                     /locus_tag="PLS88_03935"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11988..11990),aa:Thr,seq:ggt)
     gene            complement(12066..13799)
                     /gene="murJ"
                     /locus_tag="PLS88_03940"
     CDS             complement(12066..13799)
                     /gene="murJ"
                     /locus_tag="PLS88_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOU06532.1"
                     /translation="MTSRNQENSHNQEDSRNKENSRNKENSHEQVNSHGLVGFSEDTA
                     AELASQASSKMVRNAGKLSLLTFVSRVLGLIREMTKARFLGTGIYSDAFTVSFIIPNF
                     LRRLFAEGSVTVAFIPTYKGYLHAHNDSETHEFLSASLTVLSICVTIVVAIGIAFAPL
                     IVRLFGSDPAETAILTRIMFPFLAFVSIAALLQGILNAHEVFGPSGFAPILFNVCFIV
                     VPWILGNRLGNPARSMAVGVVIGGLAQALVQLPAVLRLGTHIGFMNPIRAFKNPGMRK
                     VFVLIAPTILGMAAYQVNDLVSTAYASRAGTGVASSLQYSLRLQELILGIFAVSAGTV
                     LLPRLADTVRHGAWTEYSKTLGSTMRSLLLLTVPVAVFSMAFTVRIVTLLFKSGDFSD
                     VSVQLTASAFFWHQSGLVFIAMNRLIAPAFYARSNTKTPTWAGIASFAVNIVLVLLLA
                     FRFQGPGIAFALSFSSAVNTVLLVWALIKDNIEGIRNELWLSLKYMLKMLVFSAIAIV
                     PAIFVDKAVSKVVAESGSRLVYAGLPLVTSTLVFAAIGIALLVLTKDTVAASITTALS
                     RRSRGKNASQS"
     gene            complement(13892..14707)
                     /locus_tag="PLS88_03945"
     CDS             complement(13892..14707)
                     /locus_tag="PLS88_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOU06533.1"
                     /translation="MRVLSVLATAFILAFILVVIVSCSGQKNNRLSSLPTDIRTQVEE
                     ALSAVQFSDTDRERVLDAINKDPSGFVVLFREVCEVMKKDPDLLYRVDKQKALSAQYV
                     PMDLVDLDGAFPYIVSKKGMKLRKPAQEALVRMAEMAKKDGITLVVSSAYRSYDYQKT
                     VFEQNVNEMGKAEAERVSAVPGMSQHQLGTAVDFGSISDEFAGSAAERWLASNAGKFG
                     FSLSFPKDMENITGYKWESWHYRYISPPAVEMQERFFLGIQQYLIEFLAALPN"
     gene            complement(14743..15522)
                     /locus_tag="PLS88_03950"
     CDS             complement(14743..15522)
                     /locus_tag="PLS88_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOU06534.1"
                     /translation="MKSDPQLKLAVLIDADNTQPTIIDGLLAEIAKYGVASVKRIYGD
                     WTNPNLRGWKERLLEYAIQPVQQFAYTTGKNSTDSAMIIDAMDLLYTENLDGFCLVSS
                     DSDFTRLAARLREDGKLVLGFGQRKTPKPFVAACDKFIYTEILQANVGETEEGRESSS
                     EKESGKGSPPQADIKSDTKMKALLASAVDEAADEFGWAYLGEVGTYLANRLPEFDPRN
                     YGFRKLGDLIKAVNLFEIDERTNPMVPGKQVYLRLKQRGKS"
     gene            complement(15542..16972)
                     /locus_tag="PLS88_03955"
     CDS             complement(15542..16972)
                     /locus_tag="PLS88_03955"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOU06535.1"
                     /translation="MRRSFERSLRVDAEDIFRAAVDRVMPSRLFSYCLSLEGSILSLY
                     DGEKPQLYDLSKYDHVVIAAFGKASIPMAVSLASILGERVTEGLVVFKAPESGQKSRF
                     PEDIENLFASRSIRYIEGSHPVPDERSVQAGREMLNLAMRVRKWEEVGKRTLVFVLVS
                     GGGSALLAVPASGISLEDKAEATRLLLACGATIHEINTVRKHLSAIKGGLLAKAFSPS
                     RIIALILSDVMGDDLDTIASGPTVPDTSTWQDVKSIFDRYALLDKLPRSIVQAVEDGL
                     SGIRPETPKPEDPTFDLCSTILVGTNYHAIQEAERKSKELGYNTLVLGSRFSGEAREI
                     GKVFSGIVQDIILHGVPVCTPACIIAGGETTVTLRGMGKGGRNQEMALSVLNEFNHFP
                     RSVRSGLEKTLFLSAGSDGNDGPTDAAGAFADIDTLENARRLALNPSEFLANNDSYTF
                     FYEVGGLFVTGPTGTNVCDIQLLIVR"
     gene            complement(16969..17679)
                     /locus_tag="PLS88_03960"
     CDS             complement(16969..17679)
                     /locus_tag="PLS88_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06536.1"
                     /translation="MNKFYANICERFGLNAYVMGDYQKAESWFRKLEQSEPNSIRVLR
                     NLGVISLALGRSEEAERYLLREEKLYGPSFYRHSALGDLAYATGKRKEAAKRYRLALK
                     DPECAEGGKAFHMREFIEQRLAMSEDEKRFANAMKAMEIFKKAEELRESGEHLEAIKY
                     FMESFALDETNWPALNNAASIHLNALKNPAEAEPLFRQAFELSRSPQVARNLDLCIQA
                     LEKSQKNQGQKKRWRVQA"
     gene            complement(17700..19031)
                     /locus_tag="PLS88_03965"
     CDS             complement(17700..19031)
                     /locus_tag="PLS88_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HOU06537.1"
                     /translation="MVSGVVALFLLLLAVIAIVVLTGKYKINAFLVLIGVSFVFGLLI
                     GLPALDVVAGIKNGFGGTLTSIGIVIVAGTIMGTILEKTGAALSMTKAILKVVGKSRA
                     PLAMNIAGYIVSIPVFCDSGYVILNPLNKALAKESGISMAVMAVALSTGLYATHTMVP
                     PTPGPIAAAGALGADLGKVILLGLIAAIPASLSGLLWATKFAKRYEIEPEIHETYSEI
                     VKKFGKLPNTFLSFLPIVLPIVLILLKSVAEFPSKPFGNGDFRVFLSFIGDPVTALIL
                     GVLCSLLLVQKNELKNAISDWMGEGIKESAIILVITAAGGSLGAIIKVSPITDFIKTN
                     MASMHLGILLPFIISAALKTAQGSSTVAIVTTAGIMAPLLQTLGLDPALTTIAIGAGS
                     MVVSHANDSYFWVVSQFSGMPVNIAYRAYTSATAVEGVVAFLMVLVLSLFV"
     gene            complement(19119..>19448)
                     /locus_tag="PLS88_03970"
     CDS             complement(19119..>19448)
                     /locus_tag="PLS88_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06538.1"
                     /translation="DSVQDADSILSADSVQDAGLYERIELYTPWVEHPDKFSLAYREK
                     REQYDGFLLNRSLKDGIGLEYQDFYVADAPMLFTAEGVPREWRGTDGYSDHLPVELVL
                     SVVEGVR"
     assembly_gap    19449..19548
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19549..20404)
                     /locus_tag="PLS88_03975"
     CDS             complement(<19549..20404)
                     /locus_tag="PLS88_03975"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU06539.1"
                     /translation="MHPAFLFLSIIAMLAVGCQCSLPPLHTDNEINIVSYNVHNLFDA
                     EESGKEYPEFRPSKGNWTKELYAKRLENVVEAVRSLSSTEANGSNRCSDPDILCFQEL
                     ENEKVLFDLAGRLGHGKLRYWAISGPEESIIHTGILSRFPIVAVHSHSVTNAWGLGIL
                     RDVLEVEFECGGVGRMVIFACHWKSKLEGEAETEPARRAAAALIANRIRKLQVERPSV
                     PIIVCGDFNESPDEYLRVNRCYPTAIMCSGVGVPAMAIEGEPLWAYGSLDSVQDADSI
                     LSADSVQDA"
     gene            complement(20634..21224)
                     /locus_tag="PLS88_03980"
     CDS             complement(20634..21224)
                     /locus_tag="PLS88_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOU06540.1"
                     /translation="MKFAEPQTAETETKKPEEPLNERFLKTRTVLLVGEIDKDLSEKV
                     MRQLLLLESISEEPITLLIDSPGGDVYAGFSIFDVIRFIKAPVRIVGIGLVASAAALI
                     LIAVPKDRRFGLPNSSYLIHQPLSGMNGVATDIEIHAKELERTRLHINELLAEATGQA
                     LEKIIKDTDRDFWMNAQEAMQYGLIGKVIMSRQELG"
     gene            complement(21331..22281)
                     /locus_tag="PLS88_03985"
     CDS             complement(21331..22281)
                     /locus_tag="PLS88_03985"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOU06541.1"
                     /translation="MNNSAQRVRQIARTTIKRTIDTSLFLIKIMVPVSLLVALLGWSG
                     LLEKISVVLHPLMRLLGLPGEAALVYISGALLNNYSAIAVMSSMNLSLHHAIILAVMC
                     LISHNLIVETAVMKSIGSSGTKMVILRVGVAFLAGFILNLILPSSFSEIQLFHTTAVA
                     HSPFWPMIGAWAISTLKLIGRIILYLFVLMLAETLLEQFNLMERLSRWLGWLMRIFGL
                     QPSMSFLWIVINIVGYAYGAGIIKAARDEGRMTAKEGDLFNHHAAISHSLLEDTVLYG
                     AISLPIFWLIVPRILLSIIVVWLERLRRELYRRSLKAGVA"
     gene            complement(22278..24488)
                     /locus_tag="PLS88_03990"
     CDS             complement(22278..24488)
                     /locus_tag="PLS88_03990"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOU06542.1"
                     /translation="MQFINNGQRIAGLVVPLLSLRKNKNAACGAFSDLLSLGKIAKKW
                     GLSLIQLLPLNDTGNGPSPYAAISAFALHPIYISLSDSVDTMALLGLPFRDEVARELV
                     RAEKALNCRFGSEVGIQEQRVQFEAVLQLKINALRKAWDASRDICSSLAEAFAARESW
                     AKPYACFVALRGKFGLAPWWEWPECQNVLPSDIDKLWFEPELAEEERFRLWLQVLAQE
                     QLRRAAQAVHKLGIDIMGDIPILLAKDSADVWFQHEIFILDKQAGAPPDMYSPRGQNW
                     GFPLYDWDALRERDYSFWRQRLQAADSYYTAYRIDHVLGFFRIWAISIHESDAYLGAF
                     EPSRYLEFEELKSMGFSDERIAWLSKPHISEWKIREAEKRCLDAEVVLFADSKDAGTD
                     KKQEEEQEEKQEEERAKTKALFAQRLEELGNSCFVRIKNEPLFLFRPEIIGTADIDAL
                     FRPLYAEFPKVAQILDEYASTMRDWWIDRALYQFEPDRFVFQWSYRETTSWKSLSLQE
                     RAALEAKAEAIGAESLDIWEKRGRELLSMLVSASEMQAFAEDLGAVPPCVPKVLRELK
                     IPGLRVMRWEREWDKPNQPHIPFERYEPLSVACTSVHDSTNLRQWWQEEADRLQIWAM
                     CRALAERDKLLKSLIERCGETVPERLGPGEAAVLLRALALSGSIVAAYPVQDILACHP
                     DWRSADPRDERINVPGTTLPTNWMYRMPIDLSALVKDEDFAGFISEIIARGAQV"
     gene            complement(24490..26058)
                     /gene="radA"
                     /locus_tag="PLS88_03995"
     CDS             complement(24490..26058)
                     /gene="radA"
                     /locus_tag="PLS88_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOU06543.1"
                     /translation="MHVIGQVDVRVIGESMGASIEPARPLIYDKTNMKKPSTIYRCSA
                     CGHEEPKWLGRCPECGQWNTMVEAKTTGRFEKDKTAFPVPLESVDPALGARVSSGISE
                     LDRVLGGGFMRGSAVLLGGEPGIGKSTLLLQACSKLGAKGKALYISGEESAAQIRLRA
                     ERVGALSKQIEVFCGNDMHACLSVMDSAHPLLTVVDSIQTVHSEEAGAVPGTPNQIKF
                     CTQEFVEWAKSHDSIVILVAHVTKDGMIAGPKAAEHLVDAVISFEQAENALRVLRTSK
                     NRFGSSDELGFFLMGEAGLSELQDPSGIFMVRREGQLPSGIAVAIVYEGSRILLAEVQ
                     ALTIPSKSGIMRVYSDRIDPLRVSRIAAVLEKQTRLDFSSQEIYVNVAGGLRLTEPAV
                     DLPLACALYSARSGQALPEGCALAGELSLAGEIRPVRSMDRRTKAAHQLGFVRIIGPK
                     AVPAGEQLVDMRATRAGAGKASAAEAETAAKAVSNKPEAAKAVAAAHLGQTLPWLGAS
                     SLRESLRILWGQGT"
     gene            complement(26144..>26479)
                     /locus_tag="PLS88_04000"
     CDS             complement(26144..>26479)
                     /locus_tag="PLS88_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06544.1"
                     /translation="IALFFYLNFYGLGAYMISRDDFVFCVGYNGDTAIIDGKAKKEFS
                     GLSTMELAEKGLYRAAFASALYSKNPEEMKAFIEFFNKKAGTSYTDASQLSRLFSVYL
                     ETISKAKAL"
BASE COUNT         6621 a         6831 c         5931 g         6996 t
ORIGIN      
        1 ttccatttcg gattagctgg ttaatcgtcg gcatataagc tctagactcc ctttccttcg
       61 ctcgcttcag catcgcgcgc ggttttcttc aatataattt atattccgga ctgcaaaaac
      121 tgcggtgtct gttttagcag cggtgtagaa tacctttctc aaccattaca tgtcaagacc
      181 tatggcttcc tctgccagaa gaaagcctta taacataatc ataaatctat gccatagggc
      241 aggctcaaaa gcacctggtc atttactcct ccaacgactc attgtcttcg ctgaatcctt
      301 cgtcattatc gaaaatggtc gtttcctcga agctaatatc gctcgtacga tcttggagta
      361 tggcgggtgg agctagttcg cgctcctgtt ttcgcttttc gagcacttcc tctactagag
      421 cattgaggtc gtcattttca gcatcgaaaa gtttaagctg ccggtattgc ttcattcctg
      481 ttccagcagg gataagatgc cctattatga cattctcctt gagccctcgc agttgatcta
      541 tctctccctt tatggctgca tccgtcaaaa ctcttgttgt ctcctggaag gaagccgcgg
      601 caaagaagga atcgatcttg agagatgcac gcgttatgcc gagcagaatg gggcgcgcca
      661 ctgcggcctg tccgccttct tccatgactc gtctattttc gtcatggaat ttatgcttat
      721 ccacttgctg gccataaatg aataccgtat ctcctggatt gactatttcg accttcttca
      781 tcatctgacg gatgatgact cctatatgtt tatcattgat agttacaccc tgagcacggt
      841 agacctgctg tacctcatcc atgaggtagc gctggcattc ctgttcaccc agaatcgcaa
      901 ggatatcatg tggattcttg actccatcgc atagcatctc gccagcgaca accttgtcgc
      961 cgttgcgcac aagaagccta cggttctttg gcacaaggtg cttatgggtc ttgccaaagg
     1021 aatcgcggat attgattacg cgtttgcctt tgagtgtgcc cccaaaggtg acagtgccag
     1081 agaccagcgc aagaacggct ggcgatttag gtttgcgcgc ttcgaagagc tcgccgactc
     1141 gcggcaagcc gccggttata tccatggctt tggcgctttc ctttagaatc tttgcaagca
     1201 ttttgcctgc ccgaacggtt tcgccgtcct ctacattgac ataagcgcct tcgggaaggt
     1261 aataggtttc tatatcgttg ccagcctcgt cggttatgat tattcgcggc tcttttgtgt
     1321 cgcgctcgtt tcctacatcg gtaataatct tttccacgtt gccggtctcg gcgttgatct
     1381 cttctttcag tgtggcgcca ggcatgatgt cctcatatcg aacaatacca tcatgctcag
     1441 atattattgg gtcgttgaag gggtcgaagg tcgctatagt ctcgttggca agaagaatct
     1501 ggccgcgcga taccaccatt tcggaaccgt tgcgaatctc taccttctgc tcctgagaga
     1561 ttagcaaaag tctgttatcg ataacctttg cataggatat actcggggat tttatatagc
     1621 tgccatcggc tttttttata accgaatcgt ccttcaagac acgctgacca tctttaacaa
     1681 ggagagtatc ttcatcggta aaatcgaaga cgccaaacac tctgtttaca tagatatagc
     1741 cgcggcgagt aaataataga cgaccgagct cggtctttac acaagaacct tcgatatctg
     1801 cgatgtaaac aggataatga agtgaaatac ggttctcttc agcggctttt gtggccgcac
     1861 caccgatatg gaaagtgcgc atggtaagct gggtgcctgg ttggcctatg gattgtgccg
     1921 caattattcc aacagcctct ccgatatcga ccgttctgcc ggtggctagg ttgcggccat
     1981 agcacctgcg gcatacgcca tgttcggcct cgcaagtcag aacagtacgc accgagactt
     2041 ccccgattcc tgcttcctcg atctcttcgg ctatttcctc ggtaatctcc tcattggttc
     2101 ctactatgat ctgaccagtt ataggatggc ggatcggatc tagggtaaaa cgaccgatta
     2161 tgcgctctgc aagtgtttcg atgatttcgt cgccgtcttt gagcgctgta tatatagaac
     2221 catttatggt tccacagtca tcctcgttga ctacgacatc ttgcgcaata tctacaaggc
     2281 gcctcgtaag gtaacctgca tccgcggtct tgagagccgt gtctgctaga cctttgcgag
     2341 ctccattcgt ggagatgaag aattcgatga cggaaaggcc ttcgcggaaa tttgagcgaa
     2401 taggaagctc gattatatct ccggacggct tggccattag cccacgcatg cccgctagct
     2461 ggcggatctg gttcttgctt ccgcgtgctc cagaattggc catcatgtat atattgttaa
     2521 agccatcgcg atctttttcg agggtcttca tcatcaccgc ggtgagttct tcgttcgtct
     2581 tagaccatac ctcgacgaca cggttgtagc gctcatcctg ggttatatgg cctgcaagat
     2641 actgctgctg gattgcttcg acttccttat tggccttttc gatcatttcg gccttctctt
     2701 ttggaatgac aatatcgtcc atgccgattg tcgcgccaaa aaaagtcgca tacttaaagc
     2761 ccatttcttt gatggtatcg agcatgttca cggtaataaa agggctcgtg gtacgataga
     2821 catgctctat cagcatgcga atatctttat cggtcattgc atagtttatg aaaggaactt
     2881 cttttggtag agcttcgtta agaagaatgc ggccgcctgt cgtcttgatg aagtggttcc
     2941 cagaaagctc tgcctctttt tcataagagt tccaaactgg ttctttgggt gctgccacat
     3001 agacgagagc ctgatagtcg catgaaccat attcacaggc tagcaggagc tcatccgtgc
     3061 ttgaaaagcg cttgccttcg ccctttactc ccggcctgtg gcggcttaga aagttgatgc
     3121 caagaaccat atcctgcgaa ggatagacaa tcgtctttcc gcttgccgga tccagcaggt
     3181 tgcgactcga aagcataaga gtccagcatt cggcctgagc tgcatgggtc aaaggaatat
     3241 gaaccgccat ttgatcgccg tcgaagtcgg cgttataggc tttgcatacc agcggatgca
     3301 gcttaattgc tttgccttct acgagaagag gctcaaaggc ttgtatacca agccggtgca
     3361 gagttggggc acggttgagg agcactggat gctcctttac aacctcgtcc agcactgcaa
     3421 atacttctgg cgtttccgat tcgaccagca ttttggcttt ctttatgttg tatacgacat
     3481 ccttctccac tagctttttc attataaagg gcttgaagag ctcgagagcc atctttgtag
     3541 gaagaccgca ctgccacatc ttaagttctg gcccaacgac gattaccgat ctgccagagt
     3601 agtcgactcg cttgcccaat aggttctggc ggaagcgccc ctgcttgccc tttagcatgt
     3661 ccgccaagga ttttagaggc ctattgcccg ctccctttac ggctcggcgt cgacgtgtgt
     3721 tgtcgaatag cgcgtcgacc gcttcctgga gcatgcgctt ctcattgaag attatgatat
     3781 ccggcgcgtt gagctgtaac agttttttta gccgattatt gcggtttatt actcgacgat
     3841 agagatcatt gaggtcactt gtagcgaatc gaccaccatc tagctggacc ataggcctta
     3901 gttccggcgg aattactgga attacagtaa gaatcatcca ttcaggccta ttcccagaag
     3961 atcgaaatgc ctcgacgagc tcgatacgct tcaaaaggcg cttgtcggac ttgcttccgc
     4021 gctcgatcat tttctgtctg agttcctgag acatcttatc gagatctatc tgctcaagaa
     4081 gagagcgaat ggcttcggct cccatgccag cggtaaatgc gcccccatat ctgtccatag
     4141 cctcgcggta ttcctcgtcg gttaggagct gcattttctt gagctcggtc tctcctggat
     4201 cgattacgat atatttttca taatagagga cagatttgag tgtcgccatt ggtaggttga
     4261 gcaaaagacc catgcggctg ggcacggagc gataaaacca aatgtgcgat accgggctcg
     4321 ctagctctat atgccccatg cgctcccgcc gcaccttaga attggtaact tcgacaccgc
     4381 agcggtcgca gatgactccc ttatacctga tagacttaaa cttgccacaa tagcactccc
     4441 attccttggt cgtgccaaag atgcgttcgc agaaaaggcc atctctctca ggccgcaatg
     4501 tcctgtaatt gattgtctca ggcttcttga cctctccata agaccaggcc cgaatcatct
     4561 ccggacttgc cagatgaatg gaaatattgt caaaatcctg aatatctctc atgatacccc
     4621 ctcgggttgt aggcatttct catgcctcgg aatattgttc caatagcgcc ctctacccaa
     4681 aaaaggcgca ctaaagacag tgcaaagtcc ggcaaacatg gattgtgcaa cagcaaaatg
     4741 tgccatccta gaatgctccg gtctgcttct tgataatatc ttcatcgcgc tccgttagag
     4801 cgatctgttt acccttagag tcataaacct tgatgtccaa cgccaagcct cgaagctcct
     4861 gcaccattac attgaaggcc tcgggaattc cagcggctgt gtgtggttct ccctttacta
     4921 tagcttcgta tatcttggag cgacctgtca tatcgtccga tttgatcgta agaagctctt
     4981 gcagagtatt tgcagcccca tatgcctcga gcgcccacac ttccatttcg cccaaccgct
     5041 gaccgccaaa ttgcgccttg ccaccgagag gttgctgagt tactagcgaa taaggacccg
     5101 tcgagcgagc atgcatcttg tcatcaacaa gatggtgtaa tttaaggaag tacacgactc
     5161 ccaccgttat cggattcttg aagaattcgc ctgttttacc atcgcggacc atgaccttcg
     5221 aagtagcggg caaccccgcc tctcttagtt tttcttctat ttgttcttga gagggcgact
     5281 ggaaaacatg gcattcatac cattcattaa gagtactggc agcccaaccg agctcagtct
     5341 ccatgatctg gcctatattc atacggctcg gtacaccaag cggatttagg cagatatcga
     5401 gtggcgtccc attatctaga tagggcatgt cctcaacagg aagaacacgc gcaacaaccc
     5461 ccttattgcc atggcgacct gccatcttgt cgccctcttt gagctttcgc ttgacggcta
     5521 ctagaacctt tacagtctct tcgacacctg gctgtaggtc atcgttgttt gctctagaca
     5581 tgcgctgcac atcgattacg gttccctcga tgccatgtgg tacatggagc gaggaatcgc
     5641 gtacatcctt ggccttttcg ccaaatatcg agtttaaaag cttgaattca ggcgtagtat
     5701 ctgagtcgct ttttggggtc accttcccta caagaatgtc gccgctcttt accttggcgc
     5761 caatccgaat cacaccttgg ccatcgagat tgtccagcat cttttcgctt gtattcggaa
     5821 tgtcgcgcgt tatgcgctct gggccaagct ttgtgtcgcg cacttcgaga atgaattcct
     5881 ttatatggat cgaggtgaaa aggtcttcct ttactactcg ctcggatatt agtatggcgt
     5941 cctcatagtt atagccattc caggggacaa atcctgctag aatgttgcga ccaagggcaa
     6001 gttccccttg gttcgtagcc ggtccatctg ctatcacttg gcctttcaga accttttctc
     6061 catgctttac aatcggccta tggttgaaac aggtgtcttg gttggtccgc tgatatttta
     6121 taagctgata gacatcttta tcatcgggcg agtccggctt ttcaggtcga attacaatgt
     6181 ggttagcgtc cacacactcg acggtccccg agcgctttgc cttgaccaga acacccgaat
     6241 cataagctgt cttccattcc ataccagttc caatcctcgg aggttcggta aaaaccaaag
     6301 gcacagcctg gcgttgcatg ttcgaaccca taagagctcg gttggcgtca tcgtgctcaa
     6361 ggaatggaat cagagccgca gataccgata ttatctgctt aggcgaaaca tccatgaatt
     6421 gaatttccga tggagatcgc attgtgtagt cgccttgatg gcggcaagat atattctggt
     6481 ctagaaatct tccactcttg tcgatatgcg cggaagcctg tgcgatatag tacttgtctt
     6541 cgtcgattgc ggaaaggtac tcgatttcgt cggttacaat gccatttctt acacggcgat
     6601 acggtgcctc gaggaatcca tattcgttga ccgttgtgta agttgcaagc gatacaatta
     6661 gcccgatatt tggtccttca ggcgtctcga tggggcacat gcgcccataa tgggtgtagt
     6721 ggacatcgcg cacctcaaaa cctgcgcgtt cgcggtttag cccgcctggc ccgagagcat
     6781 tcagcctacg cttgtgcgtt agctcagcca gcgggttaac ctggtccatg aactgagaga
     6841 gctgtgaagt accaaaaaac tccttgatag cagctacgat cggcttgatg gttatgaggt
     6901 cttgtggctt tgctgtttcg aggtctttgc cggtcattct atcttttgcc gtgcgctcca
     6961 tacggctaaa agcaaccttc ataacattgg caagaagctc gccaaccgcg cgcacgcgac
     7021 gattccccag atgatctata tcgtcgatat tcgcctctcc ataatagata tttatgaggt
     7081 gtttcatcgt agcgacgata tcttccttag taagcgtatt agtttttatg tcgaaatcaa
     7141 atttcttgtt gagcttgtag cggccaacat cgcccagatc atactgccta gcgctaaaaa
     7201 acatgttatg tagatcgcgc tcggcattct ccaaagatat gggttcacct ggtctaagca
     7261 cagaatagac tgccgagatt gcatcttcct tagtaggttc atcctggtca ggcgtttcct
     7321 ttacaagctt gacatcttca cggtcgaagc agttcaagat catctcgttg tgcagcgaat
     7381 tatcatcttc gaaatctatg atttcgatag agtctacgcc taggttgatg agttcatcga
     7441 tttcgtgcag gtgaatcttg tcgccagccc ggaagaggcg tttttgctca ccatcgatct
     7501 ccatatatac aggttttgcg agcattttcc caacaagggc atctttatcg ttctgatctt
     7561 cggagagctg caccgtcttt gtggtataaa atgcagcgat gatctcttcg cgcgaattaa
     7621 agccaatggc gcgcaaaaac atggttccta ggatctttcg cttgcgatcg atcttggcgt
     7681 gaataagatc atgcttctga tcgatttcga attcgagcca gctacctcta tatggaatta
     7741 tacgagagga gaatacaccc ttgtcgtgac tgaaaataac tccaggcgaa cgatgaatct
     7801 gagaaacgat gacgcgttcc gccccattga ttatgaatgt gccgcggtcg gtcataagtg
     7861 gaatatcgcc taagtagatt tctttctgcc ttatctctcc ggtcttcttg aacacaagat
     7921 ttataactgc ctttagaggc acagaatagg tcagtgtctt ctgcttgcat tcgagttcag
     7981 aatacttgat agctttttca tcgagagaat accgttcgta ttcgatcaca agatcaccgg
     8041 acggactttc tataggaaaa gtggtacgga atacctcctc aaggcctatt ggttcgagag
     8101 gctccccctt gcgaagtctt tctctttgga gaaagtgctc gtaagacgag agttgaatat
     8161 cgatgagatc aggtatatcc attacatcgt ggaattcttt tcctatgtaa gtccgcttga
     8221 tcttctgtcc tgaatatgtc atcagttacc ctccatgcgc ccttagcgct cgggttaaag
     8281 gtcaatacgc tgatgtttat atatcgaaca tcggcgctat atatcattcc gtcatcggga
     8341 agcactcatt gccggcaaaa ctcaattagc cagaatgctt cccgaaaacg ggggatcgaa
     8401 atcccccttc ttttcatcga ctaaggccca aaatctatgg ccagacttat tttatctcga
     8461 ccgttccgcc agcagcttcg atctgcttct tgatcttgtc tgcttcctct ttagagatgt
     8521 tctccttgag gggcttgtct ccagcctcga ctaggtcttt ggcttctttc aaaccaagac
     8581 cggttatcga acggacttcc ttaataatag cgattttctt atctgcaggc acattgccct
     8641 tgaggatcac tgtaaactca gttttctcct cgactggagc agctgcggct gaggcggcgg
     8701 gagcagcaac ggccactggg gcagctgcgg ttacgccaaa tttttcttcc atcgctttta
     8761 ctagttcgga aatctcgagg acggtcatcg aagcgattgc gtcgatgatt tgttcttttg
     8821 taagtgctgc catattatct tctcctgatc tcatggtagt ccatcacaac gaatggacct
     8881 tctatcgaac tccgacagga acggcagcct ccgaagcctg acggatactc gccttttcta
     8941 aaaaacatgc taaaaaacag ctcaaagagc ctcttccttc gccttctttt cctcgattgc
     9001 cttgagaacg cgaaccagtt tagtcggaat agcattgatt acatagacga ggttctgcgc
     9061 cggtgcttgc atggtagaca ttagcattgc tattagctga ttgcggccag gtagcttgct
     9121 gaatgcctcg accttatcag catccatgaa ttctttatct atcattgcac ccttgataat
     9181 caaggcaggc gtttccttgg caaaatcgag cagaatcttg gcaacttcat tagaatcggt
     9241 cttgataaag gcaacggcgg tgggtccttt tagcacaagc tctactgcat gcgaataacc
     9301 gagctcctcg aaggccaaac gtgcaaaatt gttcttgaca acatggagtt ctacaccctt
     9361 ctcgcgcagt tgctgccgca atgtcgtaat ctgttcgact gtcaagcctc tatattctgt
     9421 aaagataaaa tcgttcgagc ccgagatcat ctctctaagc atgcctatgg cttcgacttt
     9481 tcttggttga attttcgatg ctttaatggc catagtctta cctcttctcc gatttgttca
     9541 gtgcgacttt gacgcttggc cccatggtcg gcgtcaagaa taccgaagcg acaaaatcgc
     9601 ccttgacatc agaagggcgc ttgcgattta cctcttctac aagcgtgctt agattctcgg
     9661 caattttctc ggcctccatg gataccttgc caactggcag gtgaataatc cctgacttat
     9721 cggtgcggta ttcggtgcgg ccttttctca attcgctgag cgcgctgctc agatcgtttg
     9781 taaccgtacc tgtcttaggg ttcggcataa ggccacggcg tccgagcacc atacccaatt
     9841 tgccgacatc tttcatcata tcgggtgtcg caacggctat atcgaaatca agccatccgc
     9901 ccttgatttt ctcgatatat tcatcgctcc ctgcataggt ggctcctgcc tcgagagctt
     9961 ctttggcgcg ctcgggtttg cagaatacaa gaattctctt ctctgctcgg aattgatgag
    10021 gaaggacaac tgtatcgcgt acagattggc tcttcttaag gtttagcctt atatgtgctt
    10081 ccactgtttc atcgaatttg gctgtcttga gttctttgac gagtgcacaa gcatccccga
    10141 tatcataggc ttgcgaatga tccactttct tggctacttc aatgtatttc ttgccgcgtt
    10201 tcatatcaca actccacatc cacacccatc gaccttgcgg tccccgcaac tatgcgtttc
    10261 gcggcttcga gatcatttgc atttaggtct ggcaatttct gcttggcaat ttcttcaagc
    10321 tttgccttcg agagctttgc aaccttgtcc ttctgaggaa acgaagaacc cttttctata
    10381 ccgcaagctt ttttgatgag cacactcgcg ggaggcgttt tcaggacaaa cgtgaatgtc
    10441 ttatcctgat aaatagttat aatcgctggt ataactagcc caggctccat atttttcgtg
    10501 cgctcattga actgttgaca gaacatgggc gccgaaacac catgaggacc aagcgcaggt
    10561 cctacgggag gagccggtgt cgcctttcca gccggacact gcagtttgac tattgcagtg
    10621 attttctttt tggccatagc ctttctccta tcgtggtata cccggcattg tattgccggg
    10681 gtagcgtctc cataccatgg agactcccat cattagagca aaaacccctt atctgagaag
    10741 catatcagac tctatgctgg tatgctgctt tataaagatc tctgcatccc atggagggat
    10801 gagaacctat attttttcaa cctgtgccat atcgacttct acgggagtcg atcttccaaa
    10861 aatctgcaca ctcaccttga ttttgttttt ctcggtattc acttcatcga tgactcccga
    10921 gaacgactca aagggccctt gtgttatttt gacctgctct cccacagaga atgactgacg
    10981 gaagtagaca ggacgctcgc cttttaactc gcctgtgcgc tggaatatgc gtttgacttc
    11041 ttccattgtg atgggctgag gtcttgcatt agaagacgat cctacaaaac ctgtaacacc
    11101 attgatattc cgtatggttg cgcaggttgc cttccagcca tcttcgggca gatccatctc
    11161 cacaagaata tatccaggaa gaatctttcg agatacagcg cgcttcttcc cttcttttaa
    11221 ttcagatatc tcttcggtgg gtaccttgac atcggtgacg atatctcgcg aaagctcgcc
    11281 ggaatcgatg agcattcgta tagtcttctc tatcttattt tcataaccgg agtaaacatg
    11341 gagaatatac caggattttg ccatcccgcc tctaccttat cggaaaacga cctcgattcc
    11401 cagaacaatg aggaaatcga ttaggccgag cacaagagct accgccactg ttgaaataat
    11461 gactattttt gtcgaagcga ttacttcatc tttagatggc catacaacct ttgaaagctc
    11521 tgcatagcta tctttgaaga actgaattat cttcttcatg gcggctcctt ggaccccctt
    11581 cacattgtgc cgtacacaag cacagcatca tttgcgttat tttgctacca ggccaggtag
    11641 gactcgaacc cacaacgtcc ggttttggag accggcgctc taccattaga gctactggcc
    11701 taagaccgtt tattttacct ttgtttcttt gtggagcgtg tgcttatgat cccacttgca
    11761 atatttcatg agctcaagct tttcttgtgt agtttttcga ttcttcttgg ttgtatagtt
    11821 gcggcgcttg cactcagtgc attgaagagc aattatctct gcattctgct ttttcttgga
    11881 tgccatgatt cccattcctt tcgcggcgat ccacaattgt gcacgccgga ataatcctaa
    11941 aataaactcg agcccccgaa cggaatcgaa ccgttgacct tatccttacc atggatatgc
    12001 tctgccaact gagctacagg ggcgcaaata gagcttggcg aatatatcac tcatgtaggg
    12061 atgtgtcaag attgtgatgc gttttttccg cggctcctgc gagacaacgc agtcgttatt
    12121 gaagcagcaa ccgtatcttt tgtcaacact agcaaagcga tacctatagc tgcaaataca
    12181 agcgtcgaag ttacaagagg aagacctgca tatacgagcc tagagccaga ttccgcaaca
    12241 actttagaca cagccttatc cacaaagatc gctggcacaa tagcgatagc cgaaaacacc
    12301 agcattttta gcatatattt tagagagagc cataattcgt tccttatgcc ttcgatgtta
    12361 tctttgataa gcgcccaaac cagcaagaca gtatttacag cactagaaaa ggatagcgcg
    12421 aaagcaattc ctggaccctg gaatctaaaa gccagaagca ggacaaggac gatatttaca
    12481 gcgaaactcg ctattcccgc ccatgtaggt gtcttagtat tgcttcgagc atagaaggct
    12541 ggagcgatca gcctattcat cgcaataaag acaaggccac tttggtgcca gaaaaaagcg
    12601 ctcgcggtga gctgaaccga gacatcagaa aaatcgccgc ttttgaaaag tagcgttact
    12661 atcctcaccg taaatgccat ggaaaaaacc gccacgggta cggtaagcaa gagcaaagac
    12721 cgcattgtgc tgccaagcgt ctttgaatac tctgtccaag ccccatgcct aaccgtatcg
    12781 gccaagcgag gcagaagcac ggttccagcg gagaccgcaa agatgcctaa aatcagctct
    12841 tgaaggcgta gagaatactg aaggctagat gctacccccg taccagcgcg cgaagcgtat
    12901 gcggtgctta caaggtcgtt gacctgataa gcggccatgc caagaattgt tggcgcaatg
    12961 agaacaaaaa ccttgcgcat tccaggattc ttgaaagctc ggattggatt cataaaccct
    13021 atatgggtac caagccgcaa tacggcagga agttgaacta aagcttgggc aagaccgcct
    13081 attactacac caacggccat cgatctagcg ggattaccaa ggcgattccc cagaatccac
    13141 ggcaccacaa taaaacagac attgaataga ataggcgcaa atccagaagg gccaaaaacc
    13201 tcatgcgcat tcagaatgcc ttgcagaaga gcggcaatag aaacaaatgc aaggaaggga
    13261 aacatgatac gcgttaggat tgcggtttcg gcggggtccg aaccaaacag cctcacaatt
    13321 agaggcgcaa aggcaattcc tattgccacc acgatcgtaa cgcagatgct tagaaccgtg
    13381 aggcttgcgg ataggaattc gtgcgtttcg ctatcattgt gggcatgaag ataacccttg
    13441 tatgtaggaa tgaaagctac agttaccgaa ccttcggcaa agaggcggcg aagaaagttg
    13501 ggaataatga aagagaccgt aaaggcatcc gaataaatgc cagttccaag aaagcgcgcc
    13561 ttggtcatct cgcggattag gcccagtacg cgcgaaacaa aggtgaggag cgatagcttg
    13621 cccgcatttc tgaccatttt tgatgaagcc tgcgaggcaa gctcggcggc agtatcctcc
    13681 gaaaacccga cgagcccatg cgagttcact tgctcgtgcg aattctcttt attccgcgaa
    13741 ttctctttat tccgtgaatc ctcttgattg tgcgaattct cttgattgcg cgaagtcatt
    13801 gaggttagcc tacttcaagc gcgcataaag gtgaagtcct agactatgag gaggacgcgg
    13861 tggcggatat tcttggacgg atcgttttcg actaattggg tagggctgcg aggaactcga
    13921 ttagatactg ctgaatgccc agaaagaaac gctcctgcat ttccacggca ggcggcgaaa
    13981 tatagcgata gtgccagctc tcccatttat aaccggttat attctccata tccttcggaa
    14041 aggaaagcga aaagccgaat tttcctgcat tcgatgcaag ccagcgctct gccgcgctac
    14101 ccgcgaattc atcgcttatg gatccaaaat ccacggcggt gccgagttgg tgctgagaca
    14161 ttccgggaac tgcggatacg cgctcggctt cggcctttcc catttcattg acattctgtt
    14221 caaatacggt cttctgatag tcgtaagagc gataggcaga agagacaacg agagtaatcc
    14281 catctttttt tgccatctcc gccatgcgga caagcgcttc ttgcgcgggc ttgcgcaatt
    14341 tcataccttt ttttgatacg atatatggga aagcaccatc taggtctaca agatccatgg
    14401 gtacatactg ggcggatagg gctttctgct tgtcgacgcg atacagaagg tctggatctt
    14461 ttttcatcac ttcacatact tcgcgaaaaa gcaccacaaa gccggatgga tccttattta
    14521 ttgcatccag cactcgctct cgatcggtat cgctaaattg caccgcagaa agagcttctt
    14581 ctacctgagt acggatatcc gtaggcaaag aagaaagccg attattcttc tggcccgaac
    14641 acgatacgat tacaaccaat ataaatgcca atatgaaagc agttgctaga acagacagaa
    14701 ctcgcatgac aaaatcctac cattcactta gattcgttga ccttaacttt tcccgcgctg
    14761 tttcaatctt agatagactt gcttgccggg caccatgggg ttagtgcgct catcgatctc
    14821 gaatagattc acagccttaa tcaggtcgcc aagctttcta aaaccatagt tgcgcggatc
    14881 gaactctggg agacgatttg caagataggt tcccacttcg ccgagatagg cccatccaaa
    14941 ctcgtccgcg gcttcgtcta ctgcacttgc cagcaaagcc ttcatctttg tatcgctctt
    15001 tatgtcggcc tgcggcggag agcccttgcc gctttccttt tcagacgagc tttcgcggcc
    15061 ttcctcggtt tcaccgacat tggcttgcag aatttcggta tagataaact tgtcgcaggc
    15121 tgccacaaaa ggcttaggcg tcttgcgctg accaaaacca agcacaagct tgccgtcctc
    15181 gcggagcctt gccgcaagcc ttgtaaaatc cgaatcgcta gacacaagac agaagccatc
    15241 taagttttcg gtatacaaga gatccatcgc atcgattatc atcgcgctgt ccgtagagtt
    15301 tttgccagtt gtataggcaa actgctgaac gggctggatg gcatactcca acaatctttc
    15361 tttccagcct cgaagattcg ggttagtcca atcgccatag attcgcttaa cactggcaac
    15421 gccatattta gcgatctccg cgagaaggcc atcgataatg gtaggctgag tattgtctgc
    15481 atcgataaga accgctagtt ttagttgcgg atcactcttc ataaataatc tccagaaaaa
    15541 tttatcgcac gataagtagt tgaatgtcac atacattcgt gccggtcgga ccagtcacaa
    15601 aaaggccgcc aacctcataa aaaaaagtgt aggaatcgtt attggccaga aattccgaag
    15661 gattcagcgc aagtcgacgt gcattttcga gcgtatcaat gtcggcaaag gctcctgcgg
    15721 catcggtagg cccatcgttg ccatcggaac cagccgacaa aaagagcgtc ttttccagac
    15781 ccgagcgaac agagcgcggg aaatgattaa actcatttag caccgatagg gccatttcct
    15841 gattccttcc tcccttaccc atgccacgga gagtaacggt cgtctctccg ccggcaatga
    15901 tacatgcagg cgtgcaaact ggaactccat gcagtataat atcctggaca attccggaaa
    15961 aaaccttgcc tatttcgcga gcttcgccag aaaagcgact cccaagcaca agagtattgt
    16021 agcctagctc tttgctctta cgttcagcct cttgaatagc atgataattg gtacccacaa
    16081 gtattgtcga acataaatcg aaagttggat cttccggctt tggagtttct ggtctaatac
    16141 cggagagtcc atcttctacg gcttgaacta tggaccttgg cagtttgtcc aatagtgcat
    16201 atctatcgaa tatcgatttc acatcctgcc aagtgctcgt gtctggaacc gtaggacctg
    16261 aagcaatggt atctaaatca tcgcccatca catccgaaag gatgagtgca attatccgcg
    16321 aaggggaaaa agcctttgcc agaagaccac cttttatggc agatagatgc ttgcgcactg
    16381 tattgatctc gtgaattgta gctccacaag ccaatagaag ccttgtggct tccgccttgt
    16441 cttcaagcga tatacccgaa gcaggaaccg ccagcagagc actccctcct ccagagacca
    16501 gtacaaatac aagagtcctt ttgccgactt cttcccattt ccttacccgc atggcaagat
    16561 tgagcatttc ccttccggct tgcaccgacc tttcatccgg aacggggtga cttccctcga
    16621 tatagcgaat agaacgagat gcgaaaagat tttcgatatc ctcaggaaat ctcgacttct
    16681 gtccactttc aggggcttta aagactacaa gcccttctgt aactcgttcg cccagaatcg
    16741 atgccagact tactgccatt ggaatcgaag cctttccaaa ggccgctatc accacatgat
    16801 catatttgga taagtcatag agctgtggtt tttctccatc ataaaggctc agtatcgaac
    16861 cttccagcga aaggcaatag gaaaaaagcc ggcttggcat aaccctatcc accgcggctc
    16921 ggaaaatatc ctctgcatcc acacgcaagg agcgctcaaa gctcctcctc atgcttgcac
    16981 tctccatcgc tttttctgac cctgattttt ctggctcttc tcgagagctt ggatgcagag
    17041 atcgagattc ctagctacct gagggctacg agagagctcg aaggcctgcc gaaacaaagg
    17101 ctctgcctct gcaggatttt taagcgcgtt caaatgaatc gaggcggcat tgttgagtgc
    17161 aggccagttt gtctcatcca gagcaaagga ttccataaaa tactttatgg cttccaaatg
    17221 ttcgccagat tctctcaatt cctctgcttt cttgaaaatc tccatggctt tcatcgcatt
    17281 agcgaatctc ttttcatctt cgctcatcgc aaggcgttgc tctatgaatt ctctcatatg
    17341 gaaagccttg ccgccttcgg cacattccgg gtctttgagc gcaagccgat atcgctttgc
    17401 cgcctctttt cttttgcctg tcgcatacgc aagatcgcca agagccgaat ggcgatagaa
    17461 ggatggacca tagagctttt cttctcttaa taaatatcgt tctgcctctt ccgatctccc
    17521 cagcgcaaga gaaataactc caagatttct caaaacgcgg attgagtttg gttccgattg
    17581 ctctagcttc cggaaccagc tctctgcctt ttgataatcc cccataacat aggcattaag
    17641 gccaaaacgt tcgcagatat tcgcatagaa tttgttcatt ggccttctag attatctcac
    17701 taaacaaaca aggacaatac gagcaccatc aagaacgcta ctactccttc cacggccgtt
    17761 gcggaggtgt aggccctata ggctatattt acaggcatac ccgagaattg ggaaaccacc
    17821 cagaaatagg agtcatttgc atgcgaaaca accattgagc ctgcgcctat ggctatggtc
    17881 gtaagagctg gatctagacc gagcgtctgt aaaagaggcg ccataattcc tgcagtggta
    17941 acaatagcca cggtcgacga accttgagcg gttttgagag ctgcggaaat tataaagggc
    18001 agcagaattc ctagatgcat gctcgccatg tttgtcttga tgaaatccgt gatcgggctt
    18061 accttgatga tggcgcccag agagccacct gccgcagtta tgactagaat gatagcagat
    18121 tcctttattc cctctcccat ccaatccgaa atggcattct ttagttcatt tttctgaacc
    18181 aaaagcaaag aacagagcac accgagaata agtgcggtta caggatcacc aatgaaagat
    18241 aaaaacacac ggaaatcgcc attgccaaaa ggtttggaag ggaattccgc aacgctcttt
    18301 agcagaataa gcacaatcgg aagcacgatt ggcaagaagg aaagaaatgt attcggcaat
    18361 ttgccgaatt tctttactat ttcggaataa gtctcgtgga tttcgggctc gatttcatat
    18421 ctcttggcga atttggtcgc ccaaagaagc ccagaaagag aggctggaat agccgcaata
    18481 agacctaaaa gaatgacttt ccctagatct gcacccaatg ccccggctgc cgctataggc
    18541 cctggagtag gcggaaccat cgtgtgagtc gcatatagcc ccgtagaaag cgccaccgcc
    18601 atcactgcca tcgaaatccc ggattctttt gcaagtgctt tgtttagtgg gttcaaaatc
    18661 acatagccgg aatcgcaaaa aactggaatg gatactatat agccagctat attcatggcc
    18721 aaaggagcac gcgatttgcc tacaacttta aggatggcct tggtcatcga aagcgctgca
    18781 ccggtttttt cgagtattgt gcccatgatg gtacccgcaa ctattacaat gcctatgctt
    18841 gtcagcgtcc ctccaaatcc attcttaatc cccgcaacaa catccagagc cggtagccca
    18901 atcagaagtc caaaaacaaa agatacgccg attaacacaa ggaaagcgtt tattttatac
    18961 tttcccgtca atacaacaat agctattacc gccaacaaca aaagaaatag cgccacaact
    19021 ccagaaacca tacaagcctc caaatatata atttactata tgctttgaac gccgacaagg
    19081 atgcttcatt cccccgcgac gaaggttaaa gaaagccatt atcgcacacc ttcaacgact
    19141 gaaagtacga gctcaacagg caaatgatcg gaatatccat cagttcccct ccactcacga
    19201 ggaacacctt cggcagtgaa tagcattgga gcatccgcga catagaaatc ctggtattct
    19261 agaccgatac catcctttag acttctgttg agcaagaaac catcgtattg ttcacgcttc
    19321 tctctatagg caaggctgaa cttatcggga tgctccaccc atggagtgta gagctctatt
    19381 ctttcataaa gaccggcgtc ttggacgcta tcggcgctta gtatactatc ggcgtcttgg
    19441 acgctatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gcgtcttgga
    19561 cgctatcggc gcttagtata ctatcggcgt cttggacgct atctaaactg ccataagccc
    19621 aaagaggttc tccttcaatg gccatagcag gaactcccac tccagagcac attatcgccg
    19681 taggatagca cctgtttaca cggaggtatt catcgggtga ttcattgaag tctccacaga
    19741 caattatagg aacagaaggc cgttccacct ggagctttcg tattctattc gcaatcaatg
    19801 ccgctgcagc ccgtcgagct ggttccgtct ccgcttctcc ttccagcttg gacttccaat
    19861 gacaggcgaa gataaccatt ctgcccacac caccgcattc gaattccacc tccagcacat
    19921 ctcgcaaaat gccaagcccc catgcatttg tcaccgaatg cgaatgaaca gctactatcg
    19981 gaaaacgcga caaaatacca gtatgaatta tgctctcttc aggaccactt attgcccaat
    20041 agcggagttt tccatgacca aggcgcccag caagatcgaa gagaaccttt tcgttctcaa
    20101 gttcctggaa acataaaata tctggatccg agcaacgatt gctcccatta gcctcagtgc
    20161 tggatagcga gcgaacggct tctacaacat tttcaagcct tttggcatag agctcttttg
    20221 tccaatttcc tttggaaggt ctaaattcag ggtattcctt tccgctctcc tccgcatcaa
    20281 aaaggttatg cacattgtat gacacgatat tgatctcatt atctgtgtgc agaggcggta
    20341 gggagcactg gcagcctact gccagcattg caataatcga aagaaaaaga aatgccggat
    20401 gcacctttcc tccttacaag aaagacgcta tccggcacta tcggcgcttc aaaattgcga
    20461 taaaaataaa attgtggcgc caaaaatatc ggcgctagaa ttaaatttga agcgttataa
    20521 tcaaaatttc agcgctataa atttgaagcg ccaaaaaatt acagcgccgc aattaaaacc
    20581 tgcgacgcca aaatcaaaaa gcagcaataa ttcccaaata atactccaac ttctcagccg
    20641 agctcttgtc tggacattat tacctttcca ataagtccat attgcattgc ctcttgggca
    20701 ttcatccaaa aatctctatc ggtatctttt atgatctttt ccagcgcttg ccctgtcgcc
    20761 tccgcaagca gctcatttat atgcaaacgt gtcctttcga gttccttagc atgaatctct
    20821 atatccgtcg ctacaccatt cattccagat aatggctgat gaatcaaata tgaagaattt
    20881 ggcaaaccaa agcgacgatc cttaggaacc gctatcagaa tcagcgccgc cgcagaagcg
    20941 acaagcccta tgccaacgat cctgactggt gcctttatga atctaatcac atcaaaaatg
    21001 gagaatccgg catacacatc tccaccagga gaatcgataa gcagggttat tggctcctct
    21061 gagatgcttt caagcaatag gagctggcgc attacttttt ccgagagatc tttgtcgatc
    21121 tcaccaacta gaagcacggt tcgggtctta aggaagcgct cattcaaagg ctcttcgggt
    21181 ttcttggttt ccgtttctgc ggtctgtggc tcagcaaatt tcatttcttg taaggctcct
    21241 tgcaataatg taagagactc tttatctata ctcgatttta tagtaacgat ttcgagatag
    21301 aacggcaacg atgctggcaa tctaaaaggc tcaggcaact cctgccttta gagaacgacg
    21361 atacagctct cgccgcagtc gctccaacca tacaacaatt atagaaagca gaatgcgcgg
    21421 cacgataagc caaaagattg gcaaggaaat agccccgtag agcactgtgt cttcaaggag
    21481 cgagtgcgaa atcgccgcat gatgattgaa gagatctcct tccttagccg tcattcgccc
    21541 ttcgtcgcgc gcggctttga ttattcccgc accgtaggca taacccacta tgttgatcac
    21601 tatccataga aaactcatcg aaggttggag cccaaatatg cgcattagcc aaccgagcca
    21661 gcgagatagt ctctccatta ggttgaattg ttccaaaagc gtctcggcaa gcatcaatac
    21721 aaaaaggtaa agaatgattc gtccaattag tttcaatgtc gatatagccc aagcgccaat
    21781 cattggccag aaaggactat gcgccactgc agtagtatgg aaaagctgta tttccgaaaa
    21841 cgacgaagga agtatgagat ttaggataaa accagccaag aatgcaacac ctacccgcaa
    21901 gatcaccatc ttggttcctg aagatccaat cgacttcata acggcggtct cgacgataag
    21961 attgtgagag attaggcaca ttacagccag gataattgca tggtgaaggc tgagattcat
    22021 cgagctcatg acggctatgg ctgaataatt gttgagaagc gctccagaaa tatacacaag
    22081 tgcagcttct ccaggcaggc ccaaaaggcg cataagtgga tgcaagacca cggagatttt
    22141 ttccaacagc ccgctccaac caagaagcgc aaccaaaagg ctaactggaa ccattatctt
    22201 tattagaaat agcgacgtat cgatggttct tttgatagtc gtacgcgcaa tttgccgtac
    22261 tctctgtgcc gaattgttca cacttgcgct cctctagcta taatctccga aatgaagcct
    22321 gcaaagtctt catctttaac aagcgcgctt agatcgatgg gcattctgta catccagttt
    22381 gtcggtagag tcgtaccagg tacgttgatc ctctcatccc gaggatcggc cgatctccaa
    22441 tccgggtgac aagcgagaat atcttgcact ggataggcag caacgatgga gcccgatagc
    22501 gccagtgcgc gcaacaaaac ggctgcctcg ccggggccga ggcgttcagg aactgtctcc
    22561 ccgcaacgtt cgatgagcga cttcaaaagc ttgtcgcgct ccgcaagagc ccggcacatt
    22621 gcccatatct gcaatctatc tgcttcctcc tgccaccact ggcgcaagtt tgtggagtcg
    22681 tgcacagagg tacatgcgac agaaagcggt tcgtatcttt caaatggtat atgtggctga
    22741 tttggcttgt cccattctcg ttcccagcgc attacccgta gccctgggat ttttagctct
    22801 cgcaaaacct tgggcacaca gggtggaaca gcgcctaaat cttctgcaaa ggcttgcatt
    22861 tcgcttgccg atacaagcat cgaaagaagt tcacggcctc gtttctccca aatgtccagc
    22921 gactccgcac cgatggcctc ggcctttgcc tccaaggccg ctcgttcctg gagcgaaagg
    22981 cttttccagc ttgtcgtttc tctatagctc cactgaaaga caaatcgatc gggctcgaat
    23041 tgatagagtg ccctatcgat ccaccaatcg cgcatcgtac tggcatattc gtctagaatc
    23101 tgtgcaacct tggggaactc ggcatacaaa ggcctaaaaa gtgcgtctat gtccgctgtc
    23161 cctatgattt cgggtctgaa taaaaagagc ggctcattct ttatgcgcac aaaacaggaa
    23221 ttaccgagtt cttcgaggcg ctgggcaaat agcgccttcg tcttagcccg ctcttcttct
    23281 tgcttttctt cttgctcttc ttcttgcttt ttatctgtgc ctgcgtcctt gctatctgca
    23341 aacagcacta cctctgcatc gaggcatctt ttctcggctt ctcgtatttt ccattcggaa
    23401 atatgaggct tcgatagcca agcgatgcgc tcgtccgaaa aacccataga tttgagctct
    23461 tcaaactcca aataccgaga aggctcaaag gcgccaagat aggcatcgct ctcatggata
    23521 cttattgccc agatacggaa aaagcccagc acatggtcta tgcgatatgc ggtataataa
    23581 ctgtccgcag cctgcaggcg ttgacgccag aagctataat cccgttcgcg cagtgcatcc
    23641 caatcatata gaggaaaacc ccagttctgt cccctgggcg aatacatgtc tggaggcgct
    23701 ccggcctgtt tgtctagaat aaatatttcg tgctgaaacc atacatcggc agaatccttg
    23761 gcaaggagaa ttggtatatc gcccataata tcgataccaa gcttatgtac tgcttgggct
    23821 gcgcgcctta gttgctcctg agcgagcacc tgaagccaga gtcgaaatcg ctcctcttcg
    23881 gctaattccg gctcgaacca aagtttatct atatctgatg gcaagacatt ttggcactcc
    23941 ggccactccc accaaggcgc caagccgaat tttcccctta gagcgacaaa acaggcataa
    24001 ggtttagccc agctctctcg ggctgcaaaa gcttctgcaa gggaactgca gatatctcga
    24061 ctcgcatccc atgcctttcg aagcgcattt atttttagct gtagcaccgc ttcgaattga
    24121 actcgctgct cttgaatgcc cacctcgctt ccgaatctac aattcagagc cttctccgca
    24181 cgcaccaact cccttgctac ctcatctcta aaaggcagcc caagaagagc catagtatcg
    24241 accgaatcgg agagagaaat atatattggg tgtagagcaa aggcacttat ggcagcatag
    24301 ggcgatggcc catttcccgt atcgttgagc ggcaaaagct gaatcagaga aagtccccat
    24361 ttctttgcta ttttcccaag gctcagcagg tcggaaaacg ctccgcatgc ggcattcttg
    24421 tttttcctca gcgataagag cggcactaca agcccagcta ttcgttgccc attgttgata
    24481 aattgcatat taagttccct gaccccaaag aattcgcagc gattctcgaa gagaagaagc
    24541 ccctagccag ggcaaagtct gtcccaaatg ggctgcggca acggccttgg ctgcttcagg
    24601 cttatttgaa acggcttttg ccgccgtttc agcttcagcg gcactggcct taccggcgcc
    24661 agctcttgta gcgcgcatat ctacaagctg ctcgccggcg ggtacagcct taggtccaat
    24721 aatccgtaca aagccaagtt gatgggctgc ctttgttctt cgatccatgg atcgcaccgg
    24781 ccgaatttct ccagcaagcg aaagctcacc agcaagcgcg caaccctcgg gcagagcctg
    24841 ccccgatcgc gcagaataga gagcgcaagc gagcggcaaa tccaccgcag gctcggtgag
    24901 ccgcaagccc cctgccacat tcacatagat ttcctggctt gagaagtcga gtcttgtctg
    24961 cttttcgagt accgcagcaa tccgagacac acgcagagga tcgattcgat ccgagtacac
    25021 tctcatgatg cccgatttcg aaggaatagt taaagcctgt acctcggcca acaaaatccg
    25081 cgatccttca tatacaatcg ccacagcgat gcccgaaggc aattgtcctt cgcgcctaac
    25141 catgaatatt ccagagggat cctgtagctc cgaaagccca gcttcgccca tcagaaaaaa
    25201 ccccagttca tccgaagacc caaagcgatt cttgctggtg cgaagcactc gcagggcatt
    25261 ttctgcttgt tcgaagctga ttaccgcatc cacaagatgc tccgctgctt tgggaccggc
    25321 tatcatgcca tcctttgtaa catgtgccac aagaataaca atagaatcgt ggctttttgc
    25381 ccactcgacg aattcctggg tgcagaattt tatttgattt ggcgttcctg gcactgcccc
    25441 cgcttcctct gaatgcacag tctggattga atccacaacc gtaagaagtg gatgcgccga
    25501 atccatcacg gagagacaag catgcatatc gtttccgcag aacacctcga tctgcttgga
    25561 aagagcccct acgcgctcgg ctctaaggcg tatttgcgcc gcagattcct cgccggagat
    25621 atataacgct ttaccttttg cccccagctt cgagcaggcc tgtaaaagga gggtgctttt
    25681 acctatccca ggttcaccgc ctaagagcac tgccgagcca cgcataaaac caccgcccag
    25741 cacacgatcc agttccgaaa ttccagagga cacccgcgcg ccgagagctg gatccaccga
    25801 ttctagtggc acggggaatg ctgttttatc tttttcgaag cgccctgtgg ttttggcttc
    25861 gaccattgtg ttccattgtc cgcactcggg gcagcgtcca agccattttg gttcttcgtg
    25921 gccacaggcc gaacagcgat atatggtcga aggctttttc atattggttt tatcgtatat
    25981 tagggggcgc gcaggttcaa tagatgcgcc catcgactcc ccaatgacac gcacatcgac
    26041 ttgcccaatt acatgcacat cgactcgtta agtggctcgc attgtccgct ttcgtttcat
    26101 ctaggcagtc actagtacga gcgagcacga gagcgagcac gagctataat gcctttgcct
    26161 ttgaaattgt ttcgagatag acactaaaga gcctggaaag ctgactggca tccgtatagc
    26221 tggtgcctgc cttcttgttg aaaaattcga taaaggcttt catctcttcg ggatttttgg
    26281 aatagagcgc agatgcaaaa gctgctcggt aaagaccctt ttccgctagt tccatggtgg
    26341 aaagaccgct aaattccttc ttggccttgc catctataat tgcagtgtcg ccgttatagc
    26401 ctacgcagaa cacaaaatca tcacgagaaa tcatatacgc tccaagccca taaaaattaa
    26461 gataaaagaa taatgcgat
//
LOCUS       DAOSOB010000024        25291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_1968_length_25291_cov_61.247345, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000024 DAOSOB010000000
VERSION     DAOSOB010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 25291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25291
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1968_length_25291_cov_61.247345"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1344
                     /gene="rocD"
                     /locus_tag="PLS88_04005"
     CDS             145..1344
                     /gene="rocD"
                     /locus_tag="PLS88_04005"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOU06545.1"
                     /translation="MNSQDYIALDYQYGAHNYHPIPVVISKAKGCKVWDPEGREYYDF
                     LSAYSAVNQGHLHPHIVNAVKSQIELVTLTSRAFHNDKMGPFLKKLCEYTGYEMALPM
                     NSGVEAVETAIKAARRWGVEKRGIENGKQSIICANGNFHGRTLSAVSMSSDPDSYINY
                     GPYVPGFIKIPFGDAKAVCDVIDENTVAVLVEPIQGEAGVVVPPDGYLLDLRRICTEA
                     GILFILDEIQTGFCRTGRRFAWHYENARPDIMCLGKALGGGIMPISAIVADHEVMDVF
                     TPGTHGSTFGGNPLAGAVGIAALEVLEQENLDERAFKLGERFRQALRNTKSSHFRLVR
                     GKGLLNAVVFEEGFDAYPTCIDLKESGILAKQTHGNIIRFAPPLVITEDELEEALEKI
                     IYVLDRA"
     gene            1365..2795
                     /locus_tag="PLS88_04010"
     CDS             1365..2795
                     /locus_tag="PLS88_04010"
                     /inference="COORDINATES: protein motif:HMM:NF037483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06546.1"
                     /translation="MKNFKSLISHIYFVAFFVAFLIAVYLIAIQRQETLQASVPIENR
                     TIILESLSDKNQVSSQNASQQHAKISPLPGEIFLKTIDIDIDDDEDFEQVILCKKTAG
                     STVLWMIIADFSSNLGVYLRHFEAQILASKLESITIQPLDVTGDGLSDLLIQGLDSFN
                     NQTLTIFRRHLNRGYSLIFSEAGLSINLQDSDNLAIPSIVVESLPNKAGFVSYKTYRW
                     NRNSLILEKISESPLKESNTILEGPPGSDAQSYLLWLKRLWTKNSDTSNKCSLYLDTN
                     SNALILEGQEIQQRWIIKSAERNGNRLYLTCRLSELSDLDRIFIIDAKGQNEILISII
                     DQQVSHFRRDEGWSGRYKSFDYAKDSPEYSKILSSLDHSIFLGRYLGDNHSVLVLDQS
                     KSILVINGKYREGAARVYDYDGYRLLDFLQIQSNGLVGERLLFIISIDQSENGSINRL
                     ILRPAEIGPEGARIEYRTPYIFIKTS"
     gene            complement(2792..3826)
                     /locus_tag="PLS88_04015"
     CDS             complement(2792..3826)
                     /locus_tag="PLS88_04015"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU06547.1"
                     /translation="MKRNRIQIYGDRWFLNFTVSFAFFLSLFIFIISSSSEAETYKSL
                     TQARQAASKTTSQDALRDVLLSAVSQLPVRDGIALCQEYEAKAGQNLKAELRGILGGL
                     YLLLGQTKEAASWYIKAASIDEKYIVEALRLSIAVGDQKSALLLLKNEAVLAESRTIF
                     EIWLSLYDQDYASASSKARDALNIVTKPQLRKELLLLQYFADFGQFGASPSSLAKDFP
                     NSIESDLILGKAFPSSSLILFLGFSWIKGQAPLVDYVGSSSSSSAVEEYGSTSGIQWL
                     QVGYFSSKDNAERLSKNLTNQKFRTRIIETKNQAGELRWAVHVAVEEDWKKTQSKLKD
                     LGYESYPIGP"
     gene            complement(3838..5205)
                     /gene="miaB"
                     /locus_tag="PLS88_04020"
     CDS             complement(3838..5205)
                     /gene="miaB"
                     /locus_tag="PLS88_04020"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOU06548.1"
                     /translation="MKNYHIETYGCEMNKAESAAMEMTLKEHGWEQSREQEAQLILLN
                     TCTVRATAENRVWNRIHQLTARKKEHNFVLAVVGCMAEQYKNDIREKAPGVDYVLGTF
                     QKQSFSLMLDTIANGHKIEVLEESPSYVFGEFHHEGGAFRSFIPIMHGCNNFCTYCIV
                     PYVRGREISRNPMDILREIDKLEDLGVREITLLGQNVNSYSWEKDGTFLDFPGLLKMI
                     GMHLRKKQESKDTMGRYDVPFKQSERGIGWIRFLTSHPKDFSKELIAVMAEDPFFCHH
                     LHLPLQSGSNTILSAMNRKYTREYYLSVIDIAKKAIPDLTFSTDIMVGFPGETEEDLE
                     QTLSLMQSVHFSYAFMYHFNEREGTFAAGLPNKVPEKVKKARLAQVIALQKEITASLM
                     RERIGQVDEVLIENRSKQSLKELLARTSRDEMVVFPGSPTRIGQFAQVRFISVAGNTL
                     RGEEV"
     gene            complement(5202..5558)
                     /locus_tag="PLS88_04025"
     CDS             complement(5202..5558)
                     /locus_tag="PLS88_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06549.1"
                     /translation="MESNYPVDPKTLNDKGTKGVMSSVAGLGFLGVEALLGIPIVGTV
                     ISAGLIGLGAIGLLGKTKTDKISGTVLGAAGIAGLATLFLPGLSHSLLSLGGVGLLGY
                     GLFNLVGFIRGLKKKK"
     gene            complement(5628..6464)
                     /gene="folE2"
                     /locus_tag="PLS88_04030"
     CDS             complement(5628..6464)
                     /gene="folE2"
                     /locus_tag="PLS88_04030"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOU06550.1"
                     /translation="MVDVQSFKDDRNVAIQKVGVKGLRYPITLLDKAEKYQHTTATVN
                     LFADLPHDFKGTHMSRFIEIFEEYRSDLSMPNVIKMLKKLRIELNAQTTYTDIHFPYF
                     IKKTAPVSEQTSIMNYECFYEASCSEKGHRFIAGVIVPVQTVCPCSKAISDGGAHNQR
                     GLVTLQVSLGPFFWFEDLIRIVEESGSSELFTLLKREDEKFITEKAYMQPRFVEDVVR
                     EVYLKVDALHRFPWFSVEAENFESIHNHSAYAYVEKSDRSKIEPVSGEHGRLDQLESS
                     CP"
     assembly_gap    6496..6595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6657..7541)
                     /gene="folD"
                     /locus_tag="PLS88_04035"
     CDS             complement(6657..7541)
                     /gene="folD"
                     /locus_tag="PLS88_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOU06551.1"
                     /translation="MSAVLIDGKRIAEEIRDELVQKVILLKQKGVIPGLAVILVGDNP
                     ASISYVTAKEKACAEIGMKSFETRMAENTSEETLLERIAECNRDPNVHGILVQLPLPR
                     HIDEHRVIEAIDPNKDVDGFTPVNLGRMLLDQPCFIPCTPFGIIELLKRSGVPTNGAR
                     AVVVGRSNIVGKPLANLFLRKSVNATVTVCHTGTRDLGSHVREADIVVACAGAPGLVS
                     VDMIKPGACIIDVGVNRVPDSTKKKGYRLCGDVDFEGASQIAGWITPVPGGVGPMTIT
                     MLLSNTIDAAARISNIAL"
     gene            complement(7583..8791)
                     /locus_tag="PLS88_04040"
     CDS             complement(7583..8791)
                     /locus_tag="PLS88_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU06552.1"
                     /translation="MSNIFSRRSDAGEAEPNELSEIRASRSSSGLPVKDLSNTNPTNV
                     GLDWDSSDLISLATKDNIFYYPEPRGLYRAREAISDHFASYKRNMPPDNYFLCASTSE
                     GYSWLFKLLCDTGDAVLVPKPGYPLFQHLAELESVQVLTYKLEYVHPSGWYLNLDELS
                     FILSEDHGRRIKAIVVINPSNPTGSYIRKGERTAILDLCEKYDLALIADEVFFDFPID
                     ENVERKTFVGEDRVLTFALDGLSKRMGLPQLKLAWITISGPSQKVSRAKKRLDLIADT
                     FLSVNSPIMNALPFLFEHETAFLCKSLGRIRQNYAVYLEILTGKASPHRVLCCQGGWT
                     ALIESPSYFEEDKIAQRFLEEKGIFAQPGYFFDIDNGVHFAFSLIIPAISACCWCVEY
                     RDFFEVLENS"
     gene            complement(8802..10457)
                     /gene="ftcD"
                     /locus_tag="PLS88_04045"
     CDS             complement(8802..10457)
                     /gene="ftcD"
                     /locus_tag="PLS88_04045"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOU06553.1"
                     /translation="MEKPLLECVPNFSEGRDRTKIEAIANAIRSVQGVFLLDIDPSAD
                     TNRTVYTFLGPPEAVKEAALKAARVAYELIDMRTHHGAHPRIGALDVCPFVPVSGITM
                     EECVTISKDFGKKLSEELNVPVYLYEKSATCPERQSLADIRAGEYEGLEAKLADLAWQ
                     PDFGPAHFNARWGATVTGAREFLIAYNINLNTKDKRIAHDIALSIRETGRTIKDSNGN
                     IVKIPGRLQAVRAIGWYIESYQCAQVSINILDFHKTPLHIVFDAVKEEAEARGVYVTG
                     SELIGLIPLEAICVCGQYYRQKAGKSPALSDHELVELAIQTLGLRSVQPFEPEKKIIE
                     WAIRKEDILVSKKVGSFVDIVSSDSPAPGGGSVAALAGALGSALAAMDANLTVGKKGY
                     EGFYEELSALGLKAQKLKKALLELVDEDTAAFNAVMDAMRLPKSTEAEKRARDVAIET
                     ANKEAASVPLKTSRFCLEAMRLCLEAARTGNSNSATDAATGCLMAWAGLSGAVLNVRI
                     NIKTLSDEQFTCEARKECDYLETEAAQLRMELEKSILGILGLR"
     gene            complement(10459..11139)
                     /locus_tag="PLS88_04050"
     CDS             complement(10459..11139)
                     /locus_tag="PLS88_04050"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOU06554.1"
                     /translation="MTDIKAEQEDLGISGEQRLPFSAAILNGGHARRFGGIDKQEVEI
                     DGIPIGLMLASQLSKIAQELLIVGKPQKSYESLGASQFEDDIKEGGPACGLLTALIHA
                     SFDWLCLCAVDMPFVSLELIRYLYVQSMSKDVDIVLCKTEKSIQPFFAFYNKHLVFEL
                     YSYIQSSSGLSLWHFIKIKNHLIIHENEIISLCDPRLVFQNINNRRSLEEARRQVLGL
                     NTFHIMEE"
     gene            11151..12047
                     /locus_tag="PLS88_04055"
     CDS             11151..12047
                     /locus_tag="PLS88_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOU06555.1"
                     /translation="MTCLAKFMQILEWADWDTPPDQMNCSLSFQFIIQTIKELIPAIE
                     SAQLSSKELDSYKIHDLLDKAIRLYLMTPAIVNVLLNYKICVEHGLPLHPAVYYELKE
                     ARKYRIRHSLAEIQQANEQYWQSIEVARLCYQCLPQAIPAIDKLYSKIPPSIFSFLYT
                     AVQDRYTWLGSQPSLLSELAAKISKEFKPSLIVAAAHGSIMPALILSELLEIPLYFIR
                     FSMFKRHDEEPIISLSDQAWLFDYRNKDVLLYDEDVAGGNTLDLFLKRLSPLFGEVKT
                     ACSIRHAGSSIHTDFSGRVWWD"
     gene            12367..13011
                     /locus_tag="PLS88_04060"
     CDS             12367..13011
                     /locus_tag="PLS88_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOU06556.1"
                     /translation="MSQKTKLNYVPSIRRFPSYLHIIRAFQRIGEPYISGTWIANELN
                     LEPIQVRKDLSITGIVGKPKKGYPVDQLIAAIEHYLAWDIEQRAMIVGAGNLGSALTG
                     YQEFRNHGLHIVAAFDSDPLKVGKTIHQIQVYQMNQLKEIVERMKITLAILTVPSPYA
                     QSSCDAIIAAGIKAIWNFTNVKLKVPDDVIVQREDLSSGYAILSVMMRTKAKLS"
     gene            13035..13622
                     /locus_tag="PLS88_04065"
     CDS             13035..13622
                     /locus_tag="PLS88_04065"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOU06557.1"
                     /translation="MKIFLISGPRGSGKTSFCNALVKTAKERGILCRGCIEISQRDEN
                     GIPYRISLCDVSNGETFLAASRPIGKRDVAFEFSYDAFSYIRESYIQAIKLDAISQHA
                     HYGLDKKGEICLIDEIGPLELENSGGHLCFLEFVLRTKDPDALVLTVRTSLKYPLISF
                     LVNHEISINQICNLELDKLTISSACLDIFSFLEQR"
     gene            13705..14145
                     /locus_tag="PLS88_04070"
     CDS             13705..14145
                     /locus_tag="PLS88_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOU06558.1"
                     /translation="MRMTTRGLYAIKAILSLVAISEEQKPVSLNKIAQIENLSPEFLQ
                     QIFYKLRKGGILKSSRGPGGGFSLNKKPEEISAYDVLYAVGETLEIVPCASRRSTKKA
                     CSRFDTCYVGPFWKEMENIIVEYAKSKSLKDISLARAHPEVISD"
     gene            complement(14222..15667)
                     /gene="pyrF"
                     /locus_tag="PLS88_04075"
     CDS             complement(14222..15667)
                     /gene="pyrF"
                     /locus_tag="PLS88_04075"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOU06559.1"
                     /translation="MDFFERIERRADKMDTLLCIGLDPAFSRTEIEKRGISSCAALAL
                     EQNLRIIEGTTSYALAFKPNIAFYEALGDAGISALYKTIAQIPEEVPIIIDAKRGDIA
                     NTAEAYAQAIFGELKADAVTLNPYLGLDTLEPFLRWSDKGVFVLCRTSNPGAGFLQDI
                     MVNKDPLYLEIAKRCSALPISVGLVVAGNDIEALQKARRVAPSSWFLSPGIGAQGGRA
                     DLAFAAGARSDGKGILVASARSISEAQRPDNAALELRDEIRRARDSFLSKASKPSQKA
                     TKATDEVVSLKRDFIDALLSTECFRLGEFALKSGRKSPFYIDLRNLVADPEAMRIAGR
                     AYAYLASCCDYDIIAGIPVAGLPLATAASLEIGKPMIWPRMPFKEHGTGKHIEGRYAK
                     GDRVLLLDDLITTGASKLEAIEILRSEGLEINDLVVLIERGSEGRVDMEREGVRLHAL
                     MHVNELFESLKENGKISEVQYSELVDFGKTI"
     gene            15765..17126
                     /locus_tag="PLS88_04080"
     CDS             15765..17126
                     /locus_tag="PLS88_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06560.1"
                     /translation="MKKAFLLFALILVSLPIFAQQGTNPSFGFAANLGTDLLPDPSDP
                     SKFESWSKVGLQPEFNIGKFGIGLDLLLRFKLGTGSNTNLEIYEPDWIPQQGQNIFDV
                     YLPKILYIRYGQQWEDPFYIKMGSISDFSLGNGLIVENYSNMRFLPQRRVFGMQLGVD
                     GSLFSFPYLGLEALTGNISKFDVIGGRVYARPLAFMGESIFGKLQLGVTSVFDRDPLL
                     YTGSPYQTLATKLIYVVGADITLPLIQSPAFSLIPFGEGAYEMNKAMGAIAGIRGRAF
                     GLVSYRAQFRYLQEGFIPSYFDANYDIYRADRFVFIETNPAKNNFIPGWLASLGFDLF
                     KSSLSFGVTLDAPFRELPTTATDNPADYPHVKGSLSLGRGLIPNVDITARYEKFFLGK
                     KSNDVIKDLIDLEDSYIGMTVSYKAGTAVVSMDYAYIWNPTKNSGKGAFDVVSSLSVG
                     FEL"
     gene            17223..18989
                     /gene="mutL"
                     /locus_tag="PLS88_04085"
     CDS             17223..18989
                     /gene="mutL"
                     /locus_tag="PLS88_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOU06561.1"
                     /translation="MGNIRILPPETSRRIAAGEVIDRPSSALREIIDNAIDADAHTIS
                     VSIAQGGIEEITVADDGWGMSKEDLLLSIAEHATSKIYGPDDILKAKTLGFRGEALAS
                     IAAVARLEIVSKLRGASEGWRLSSSPLKEPIIEPFACKEGTRLTMRGLFESYPARRQF
                     LKRPQSEAFLCRSTFIERALSHPDICFRWAVSGDMDVMMPSSLKDRIIRCYTELSRFP
                     LFEVMAETSELKMHCVYADVSNYRRDRKFLQVFVNKRRVPEWNLLSLMEYEFGKYLPG
                     GAHAIAFLLIEIDPSLADFNIHPAKKEVRLKKPAEVRTVLHELLSHELVHRYGSLTQA
                     FKVANYPEFEDFVFSTKEKTSGPSLADKSHVLASEGCGKTTQRVLENGSSNLYSMHRN
                     YSQLPDDFWHRIRDDNLSALRYIGKGPGPFLVFELSDALFILDQHAAHERILYNKIKS
                     NKGESQLLLVPYLLEPYENEHYLEEAKDNLEFVGYRITRDKNTWIVDAVPAIAASAAL
                     EALVEWLSQPSSEASPIDALIASLACKAAIKDGDRLDEISALKLIQESLELPEPRCPH
                     GRPIYLAFSREQLYSMFGRSIG"
     gene            18986..19654
                     /locus_tag="PLS88_04090"
     CDS             18986..19654
                     /locus_tag="PLS88_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU06562.1"
                     /translation="MKKAIRILCVADEVDLLVYSTQICERFSDIDLILSAGDLPDGYL
                     EYIASMLNKPLVSVAGNHDKSNQDIENGNIYGSSNQRVNFGQISFKIAKENGISILGL
                     PGSIRYNKGQNQYTDAWMTFRIILMLPRLLLRRLLWGRSVDIILAHSPPRGIHDGGDP
                     AHKGFSAYRWLIRFARPYYFIHGHVHLYDMQALREMDYFNTKVVNVYGHQIITLPKEV
                     KNDR"
     gene            19644..20582
                     /locus_tag="PLS88_04095"
     CDS             19644..20582
                     /locus_tag="PLS88_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOU06563.1"
                     /translation="MTDDFHALAESDFYKARSKAITTTIFGMLRPSMSDLMPFDQAKE
                     LLKPKAETYRGITAVPIDRIVGSEGRYRDFNRFFFPKKEHLKARWTGIDELQYKDISL
                     PPVVLYEMGGVYFVRDGNHRVSVAKAKKQEFIDAEVISLQSEIQLDPEMTIEDIKKAV
                     IEYEKRRFYKETNYPNIVGADDLNFSEPGRFDTIKEHVLVHKYFLNQHISEEIPFHVA
                     LYSWHDNVYQPISQAIEAENILSLFPGRTVSDLYLFLVAHWDELKRKLGRYVAIDEAA
                     ESFKNQAKRNRNSSFEHACRIFGSISIKVENFFAKL"
     gene            20763..20933
                     /locus_tag="PLS88_04100"
     CDS             20763..20933
                     /locus_tag="PLS88_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06564.1"
                     /translation="MAQVSKNARTGSSTQRFYCSCGGEIKMKTIFENSKVKTIAECEK
                     CKRVERRPSDFK"
     gene            complement(21031..21789)
                     /locus_tag="PLS88_04105"
     CDS             complement(21031..21789)
                     /locus_tag="PLS88_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU06565.1"
                     /translation="MLVNAIVVLIIISMLFLLEFILALRCLFSDRNKRFILCAKYEQI
                     GAKIILGLFRIFHGFKISVQNPEKLEIPLRCLVISNHQSLLDIIILISFLGIERKPRF
                     VAKRELQYGIPFVSFTLRKGGHCLIKREGAPIDAMREVSKMARICTQIGACPVIFPEG
                     TRSRNGHVGEFHTAGVRKLLESGGKLPVAAIALEGGWRVANAMDFLKRFGKEPYIVEI
                     VKVYEPPRSKFDIENMLNDARHLIEKQIEELRMQ"
     gene            21935..22204
                     /gene="rpsT"
                     /locus_tag="PLS88_04110"
     CDS             21935..22204
                     /gene="rpsT"
                     /locus_tag="PLS88_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOU06566.1"
                     /translation="MSTSNLSAEKRQRQNEKRRLANKSTKTSIKSAARKVVIASEKKD
                     AAAAKEALSEMIKRIDSAARKGIVKKNTAARKKSRMQRLVNKIDQ"
     gene            22250..22594
                     /locus_tag="PLS88_04115"
     CDS             22250..22594
                     /locus_tag="PLS88_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU06567.1"
                     /translation="MAEKLTKAELIDALYDSLSPTSRTTRKEIHELIDGLFAEIKGAI
                     LDGKIVELRGFGTFEVKLRKGRSKARNPKTGEIVSVTDHGVATFRPGRELKKAAWEMD
                     IERIPKSFRKRV"
     gene            22653..23039
                     /locus_tag="PLS88_04120"
     CDS             22653..23039
                     /locus_tag="PLS88_04120"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU06568.1"
                     /translation="MKKLNLEPSEPYGTHIGPALILNGILRSSQPLDLHCVFHGSIEG
                     TFIKISRNAKIDVDLLRARQVIIAGQFKGYMSVSEEIVILPNASVKSDIKVSCLHIIE
                     GAQFEGKIRMAEFSPDDKSQTEDRRH"
     gene            23400..24977
                     /gene="rho"
                     /locus_tag="PLS88_04125"
     CDS             23400..24977
                     /gene="rho"
                     /locus_tag="PLS88_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOU06569.1"
                     /translation="MQDIQRSLNDVHVASPLKNQQQKVRKPVTKIRLKKKPTQNVDLV
                     EESQSNGSTTTAEALEEQPNGFVVAAMPLDDHQHQKLVELPKSELESRVEQRIESRAR
                     LLINDLSKMGIIELRELGAKYGINHDLLITLKKQELIFNILKGHTEHGGIIYAYGSLE
                     ILPDGYGFLRSPQNSYLPGTDDIYISPSQIRLFNLKTGDTVYGQIRSPKEGERFFAML
                     RIEQINFDDPSVAQNRIPFEDLTPLYPTQKLDLETSTEEISTRIINLFCPIGKGQRGL
                     IVSPPRTGKTILLQKIANAITANHPEVYLIVLLIDERPEEVTDMERTVKAEVISSTFD
                     EQATRHVQVAEMVLEKAKRLVEHKRDVVILLDSITRLARAYNQTVPTSGKILSGGVDS
                     NALHKPKRFFGAARNIEQGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMEINLDRRL
                     SDRRLYPAINIKKSGTRKEELLISEEELQKIWVLRKVINPMDDIEIIELLIDRMMKTK
                     NNDAFLKSMNTPYSSME"
     gene            25073..25288
                     /gene="rpmE"
                     /locus_tag="PLS88_04130"
     CDS             25073..25288
                     /gene="rpmE"
                     /locus_tag="PLS88_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOU06570.1"
                     /translation="MKKGIHPKYELTTITCACGNVIETRSTAKNIQVEICSACHPFFT
                     GKQKLVDTAGRIERFNKKYGIKANDND"
BASE COUNT         7435 a         5510 c         5090 g         7156 t
ORIGIN      
        1 tgttgtttat gaatacagag ctatataatg cataattatt tatactattt ctaattatta
       61 ttatcacttc cgtattcaat agcttcgggg tataattaag atcaaaaaga tatacgactc
      121 caataatctc aaaaggaggc aaccatgaat tcccaggatt acatagctct ggattaccaa
      181 tacggagcgc ataattatca tcctattccc gtcgtcattt caaaggccaa aggatgcaag
      241 gtttgggatc ccgaagggcg agagtactat gattttctgt cagcatattc agcagtaaac
      301 caaggccatc ttcatccgca tattgtcaac gctgtaaaaa gtcaaatcga gctagtaaca
      361 ctcacttcaa gagcgttcca caatgacaaa atggggccat tcctcaagaa actatgcgag
      421 tatacaggct atgaaatggc tcttcctatg aactctggcg tagaagccgt agagacagca
      481 atcaaggctg caaggcgatg gggcgtggaa aaaagaggga tagagaatgg aaaacagtcc
      541 ataatctgcg ccaatggcaa ctttcatggt cgaacgctaa gcgcagtatc gatgtcgagc
      601 gatcctgatt cctatatcaa ctatgggcct tatgtgccag gttttataaa aattcctttt
      661 ggagatgcca aggcagtctg tgatgtcatc gatgagaata cagtagccgt gctagtagag
      721 cccattcaag gagaagccgg tgttgttgtt cctcctgatg gctatctctt ggatctcaga
      781 agaatctgca ccgaagcagg gatcttattc atactcgatg aaatacaaac tggtttttgc
      841 agaacaggcc gtcgtttcgc ctggcactac gaaaatgctc gtcccgatat catgtgtctc
      901 ggaaaagctt taggtggtgg aatcatgccg atttccgcca ttgttgcaga tcatgaagtt
      961 atggatgtat tcacaccagg gacacacggt tcaacttttg gtgggaatcc actagctggt
     1021 gcagttggaa ttgctgctct cgaagtcctc gaacaagaga atctcgatga aagagcattt
     1081 aagcttggcg aacgttttag gcaagcctta agaaatacaa aatcatctca tttccgcctg
     1141 gttcgtggga aaggtctcct aaatgcagtt gtcttcgaag agggctttga tgcctatcct
     1201 acttgcatag acttaaagga atctggcata cttgctaaac agacgcacgg aaatataata
     1261 cgctttgccc caccgctcgt cataacagag gacgagctcg aagaagctct tgagaaaata
     1321 atctacgtgc tggacagagc ctagaatcta atttgcagag gatgatgaag aatttcaaat
     1381 ccttgatcag ccatatatat tttgtggctt tttttgtggc ctttttgata gcagtatact
     1441 tgattgctat acaaagacaa gaaaccttac aagcttcggt gccaattgag aatcgcacca
     1501 ttatcctcga aagcttatca gataaaaacc aagtttcgtc tcaaaatgct tcacaacaac
     1561 atgcaaagat atcgcctctt ccgggtgaga tctttctcaa gacaatagac atagatatcg
     1621 acgatgatga agatttcgag caagttattc tatgcaagaa aactgccgga agcacggtct
     1681 tgtggatgat aattgccgac ttttcatcaa atcttggtgt ctatctcaga cattttgaag
     1741 cccaaatact ggcttcaaag ctagagagca taaccataca gccacttgat gttacagggg
     1801 atgggctttc cgatcttctt attcagggtc tggatagttt taacaatcaa actctgacaa
     1861 tatttcgtcg acatctaaat cgagggtata gtcttatatt ttcagaggca ggcttgtcta
     1921 taaatcttca ggactctgat aatcttgcta tcccatctat tgttgtagaa tcattgccaa
     1981 ataaagcggg tttcgtttct tataagacct atagatggaa tagaaacagc ctcattttag
     2041 aaaaaatatc ggaatctcct ttaaaagaga gcaataccat cttagaaggt cctcctggaa
     2101 gcgacgctca gtcatatctc ttatggctca agcgactttg gacaaagaat tcagatacct
     2161 ctaacaaatg ttcgctatat ctagatacga atagtaatgc actgatatta gagggccaag
     2221 agattcagca acgatggata atcaaaagtg ctgaacgcaa tggcaatcga ctatatctta
     2281 cctgtaggct ctcagagcta agcgatctcg atcgaatctt tataattgat gccaaaggac
     2341 aaaacgagat attgatcagc ataatcgatc aacaagtatc tcatttccgc cgcgatgagg
     2401 gttggtctgg gagatacaaa tcgtttgatt atgcaaagga ctctccagaa tatagcaaga
     2461 tattgtcgag tcttgatcat tcgatatttc taggccgtta tcttggtgat aaccattctg
     2521 tgctggtgct tgatcagtcc aagagcatcc ttgtaataaa tgggaaatac agagagggag
     2581 ccgcaagagt ctatgattat gatggatatc gattgcttga tttcttgcag attcaatcga
     2641 atggacttgt cggagaaagg ctactcttca ttatctctat cgatcaatct gagaatggct
     2701 caataaatcg tctcatcctt cgaccagccg agatcggacc agagggtgct agaatagaat
     2761 atcgaacacc atatatcttc atcaaaacaa gttaggggcc tataggataa gattcatatc
     2821 caaggtcttt gagcttggac tgtgtttttt tccagtcttc ttcaactgcc acatggacag
     2881 cccatcttag ctcgccagct tggtttttag tttctatgat ccttgtcctg aacttctgat
     2941 tagtcaggtt tttagatagc cgctcagcat tgtctttcga agaaaaatag cccacctgca
     3001 accattggat tccgcttgta gagccatatt cttctaccgc tgaagaactg ctcgatgatc
     3061 ccacataatc aaccagaggt gcttgaccct taatccacga aaaccctaaa aaaagtataa
     3121 gcgatgaaga agggaaagct tttcctagaa tcaaatccga ttctattgag ttaggaaaat
     3181 cctttgccaa gctggatgga ctagccccaa actgaccaaa atccgcaaaa tattgcagca
     3241 gtaaaagctc ttttcgcaat tggggctttg taacgatatt taatgcatct ctcgcttttg
     3301 aagaagctga agcataatct tgatcataaa gagaaagcca tatttcaaaa atagtccgag
     3361 attccgccaa gacagcctcg ttttttaaaa gcagtaatgc gcttttctga tcgccgactg
     3421 ctattgaaag ccttagcgcc tcaacaatgt atttctcgtc gatagatgcg gcctttatat
     3481 accacgaagc agcttctttt gtttgcccaa gaagcaaata tagtcctcca agaatgcccc
     3541 tgagctcggc tttcaagttc tggccagcct ttgcctcata ctcttgacaa agggctattc
     3601 catctctaac aggtagctgc gaaactgccg aaagcaagac atctcgcaag gcatcttggc
     3661 ttgtagtctt tgacgcagct tgtcgtgctt gagtcaagct tttgtatgtc tctgcctccg
     3721 aggatgagct aataataaaa ataaataatg ataggaaaaa tgcaaatgag actgtgaaat
     3781 tgagaaacca gcgatcgccg tatatctgta ttctgtttct cttcacaatt cttatcttca
     3841 cacttcctcg cctcttaatg tattgccagc aaccgatata aaccttactt gggcaaattg
     3901 cccgattctt gttggagagc caggaaagac caccatctca tcgcgtgatg tccttgccaa
     3961 tagctctttt aatgattgct ttgatcggtt ttcaatgagc acttcatcga cttggccaat
     4021 ccgctctctc ataagagaag cggttatctc tttttgaaga gcaattacct gagcaagcct
     4081 tgcttttttt actttttcag gtactttatt tggtagtcca gcagcaaatg tgccttcgcg
     4141 ttcgttgaag tgatacataa aagcataaga gaaatgaaca ctttgcatga ggcttaaggt
     4201 ctgttcaaga tcttcttcgg tctcaccagg gaagccaacc attatgtcgg tagaaaatgt
     4261 aaggtctgga attgccttct ttgcaatatc gattaccgaa agatagtatt ctcgagtata
     4321 tttgcgattc atcgcggaga gtatggtatt agagcctgat tgcagaggca aatggaggtg
     4381 atgacaaaaa aaaggatcct ctgccataac tgctataagc tctttagaaa aatcttttgg
     4441 atggcttgta agaaaacgaa tccagccaat tccacgctca gattgcttaa atggcacatc
     4501 gtaacgtccc atcgtatctt tgctttcttg cttttttcgc aagtgcatcc caatcatctt
     4561 taggagtccg ggaaaatcta aaaatgtccc atctttttcc caactatagg aatttacatt
     4621 ctgtccaaga agagttatct cacgaacacc aagatcttcg agcttgtcta tttctcgaag
     4681 aatatccata gggtttcgcg aaatctcgcg cccccgtaca taaggcacta tacagtaggt
     4741 gcaaaaattg ttgcagccat gcattatggg aataaaagat cgaaatgccc caccctcatg
     4801 atggaactca ccgaaaacgt aactcgggct ctcctcgagg acttcgatct tatggccatt
     4861 tgcaatggtg tcgagcataa gagaaaatga ttgcttttgg aatgttccaa gaacatagtc
     4921 gacgccggga gctttttctc gaatgtcatt tttatactgc tctgccatac aaccaacaac
     4981 ggccagaacg aaattatgtt ctttcttgcg tgcggttaat tgatgaatac gattccatac
     5041 gcggttttct gcagttgctc ttacggtgca cgtattcaat aagattagct gagcttcttg
     5101 ctctctagat tgctcccatc catgttcttt aagagtcatc tccatagctg ccgattcagc
     5161 cttattcatt tcgcagccat aggtctcgat atgataattc ttcatttctt ctttttgagg
     5221 cccctaataa atccaacaag attgaaaaga ccatatccaa gaagtcctac gccacctaag
     5281 gaaagcagcg aatgtgataa accaggcaaa aacagagtag caagtcctgc aatgcctgct
     5341 gcacctagca ccgtgccgga aatcttgtcg gtcttggtct ttcctaagag acctatagca
     5401 cctagcccta tgagccccgc ggatattaca gtcccaacta ttgggatgcc aagcaaagcc
     5461 tcgacaccta aaaagccaag gcctgcaacc gaagacatta caccctttgt ccctttatcg
     5521 ttcaaagtct tgggatcgac cggataattg ctttccatat cttttcctct cttgatccaa
     5581 tatgcatctt actccaaaat ccttaacatt gatcgacatt cctcacatca agggcaagaa
     5641 gattcaagtt gatctagcct tccatgttcg ccacttactg gttcaatctt cgaacgatca
     5701 cttttttcaa catatgcata agcagaatgg ttatggatac tctcaaaatt ctccgcttca
     5761 accgaaaacc atggaaagcg atgaagagca tcaaccttta aatatacttc tctcacgaca
     5821 tcttctacaa agcgaggctg catataggcc ttctcagtta taaacttttc atcttcgcgt
     5881 ttcaggagag taaataattc gctcgagcca gattcctcaa ctattcggat taagtcttca
     5941 aaccaaaaaa aggggccaag agatacttga agtgtcacaa ggcctcgctg gttatgtgct
     6001 cctccatctg aaatcgcttt ggaacatgga cagactgttt gcacgggcac tattacacca
     6061 gcaataaaac gatgcccttt ttcagagcag cttgcttcgt aaaagcattc atagttcatt
     6121 atagatgttt gttccgaaac aggtgccgtc tttttgataa agtatggaaa atgaatatct
     6181 gtgtaggttg tctgcgcatt cagctctatt cttaactttt ttagcatttt aatcacattg
     6241 ggcatcgaga ggtcgctgcg atattcctca aagatctcta tgaatctgct catgtgagta
     6301 cctttgaaat catgcggaag gtctgcaaat aaattgaccg tagctgttgt gtgctggtat
     6361 ttttcagctt tgtcgagcaa ggttatgggg tatctgaggc ctttgactcc gactttctgg
     6421 atcgcaacat tgcgatcatc cttaaacgat tgaacatcta ccataatcta aacctatatt
     6481 tcgcatcaag gcgctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatcaa
     6601 ggcgcttaat aatatctaaa acctatcttt tctctgccta atgtagactt ggattttcac
     6661 agcgcaatat ttgaaatcct cgcagcagca tcgatggtgt ttgagagcaa catagtgatg
     6721 gtcattggcc ctacccctcc tggtacaggt gttatccagc ctgcaatctg cgaagctcct
     6781 tcgaaatcaa catcaccgca aagccgataa cccttctttt tagtagaatc tggcacacga
     6841 ttgactccta catctataat acaagcacct ggcttgatca tatccacaga caccaaacct
     6901 ggtgctcccg cgcatgccac tacaatatcc gcttcccgaa catgagaacc aagatccctc
     6961 gtcccagtgt ggcagacagt tacagttgca ttgaccgatt tccggagaaa aaggtttgca
     7021 agcggtttgc ctacaatgtt cgagcgcccg acaaccactg cccttgctcc attggtcggt
     7081 acaccgctcc tcttaaggag ttcaattatt ccaaacggcg tacagggtat gaaacaaggt
     7141 tgatcaagga gcatgcgacc gagattgact ggagtaaaac catctacgtc cttgttggga
     7201 tcgatagcct cgataacgcg gtgctcatcg atatgtctag gaagaggaag ctgaacgagg
     7261 atgccatgaa catttggatc tctattgcat tctgcaattc tttcaagaag ggtttcctct
     7321 gatgtattct cggccatcct tgtctcgaaa ctcttcatac cgatttcagc gcaggctttt
     7381 tcttttgcgg tcacataaga aatcgaagca gggttatccc ccacaagaat cacagcaaga
     7441 cctggtatta ctcctttttg cttgagtaag atgactttct ggacaagctc atccctaatc
     7501 tcctcagcaa ttctctttcc atcgataagt actgcagaca taactcctcc aaccttcaag
     7561 atactatatt tctaggattt cgtcatgaat tttctagtac ttcaaagaaa tccctgtatt
     7621 ccacgcacca gcaacatgcg ctgatggcag gaataatcaa actaaaggca aaatgcaccc
     7681 cattatctat gtcaaaaaaa tatcctggtt gtgcaaaaat ccctttttct tcgagaaatc
     7741 gctgagctat tttatcttct tcaaaataac tcgggctttc aattagagca gtccatccgc
     7801 cttgacagca aagcactcta tgcggactag ctttacctgt caagatttcc agatagactg
     7861 cataattctg gcgaattctt cctagcgact tacataaaaa tgccgtttca tgttcaaaga
     7921 gaaaaggcag ggcgttcatt atagggctgt tcacagaaag aaaagtatct gcaatgaggt
     7981 caagtcgctt ttttgctcta gataccttct ggcttggccc agagatagtt atccatgcaa
     8041 gcttcagctg aggcaatccc attcgtttgg agagtccgtc taatgcaaaa gtaagcactc
     8101 tgtcttcccc tacaaatgtc tttcgttcga cattctcatc tataggaaaa tcgaaaaaaa
     8161 cttcatccgc tatcaatgcg agatcatatt tttcgcatag atccagaatt gcggttcttt
     8221 ctccttttcg gatatatgaa ccagttggat tgctgggatt gattaccaca atggccttga
     8281 tacgccttcc atgatcttcg gaaagaataa atgaaagctc gtctaggttc aaataccagc
     8341 cagaaggatg aacatactca agtttataag tcaggacttg caccgattct agttccgcca
     8401 agtgttggaa aagaggataa ccaggttttg gtacaagtac cgcatctcca gtgtcgcata
     8461 gcaatttgaa aagccatgaa tatccctccg aagttgatgc acagagaaaa tagttatcgg
     8521 gaggcatatt tcgcttatat gaagcgaaat gatccgagat ggcctcccga gcacgataaa
     8581 gccctctagg ctccgggtaa tagaaaatat tatctttagt tgccagagag ataagatcag
     8641 agctatccca atcaagccct acatttgtgg gatttgtatt cgatagatct ttgactggaa
     8701 gcccagagct ggacctcgat gcacgaatct cagaaagctc atttggttct gcttctccag
     8761 catcggaccg tctggaaaaa atgttactca tcgaaataaa cctatcgaag cccaagaata
     8821 cctaatatac tcttctcaag ctccatccga agttgagccg cctcagtttc taaataatcg
     8881 cattctttcc ttgcctcaca agtgaattgt tcatcagaca gcgtttttat gtttattcta
     8941 acattcaaga ctgctccaga aagccctgcc catgccatga gacatcctgt agctgcatct
     9001 gttgccgaat tcgagttgcc agttcgagct gcttcgaggc ataagcgcat tgcctcgaga
     9061 caaaaccgag aagttttaag aggcacgcta gctgcttctt tgttagctgt ctcgattgcc
     9121 acatcacgcg ctctcttctc ggcttcagtc gattttggaa gcctcatagc atccataacc
     9181 gcattaaaag ccgcagtatc ttcatctaca agttcaagca aagccttctt gagtttctga
     9241 gccttgagac ctaatgctga caattcttca taaaagcctt cgtatccttt cttgcctacg
     9301 gtaagatttg catccatggc agccagagca gatccaagag ctcctgccag agcagccaca
     9361 gaccctcccc caggtgctgg cgagtctgaa gagacaatat ctacaaagga gccgaccttt
     9421 tttgaaacaa gaatgtcttc tttccttatt gcccactcta ttatcttttt ttccggttca
     9481 aaaggttgta cggatcgcaa acctagtgtc tgtatggcta gctctacaag ttcgtggtcc
     9541 gaaagtgcag gacttttgcc tgctttctgg cgatagtatt gcccgcaaac acagatagct
     9601 tcgagaggaa taagtccaat aagctcagaa ccagttacat atacacctct ggcttcagct
     9661 tcttctttaa ccgcatcgaa tacaatgtgc aatggggttt tatgaaaatc gagaatattg
     9721 atagagacct gagcacattg gtaggattcg atataccatc cgatagcacg gacagcttgc
     9781 aatctgcctg gaatcttgac aatatttcca ttactatcct ttattgtacg gcctgtttct
     9841 cgaatactta atgctatatc atgagctatt ctcttatctt tggtattgag atttatatta
     9901 taagcaatca agaattctct tgcaccggtt acagttgcac cccagcgagc attgaaatga
     9961 gcgggtccaa agtcgggctg ccatgccaga tccgcaagtt ttgcctccaa tccttcatat
    10021 tcgccagcac ggatatcagc aagactctgc cgttcaggac aggtcgccga tttttcgtag
    10081 agatagactg gcacattaag ctcttctgag agctttttgc cgaaatcttt tgaaattgtt
    10141 acgcattcct ccatggtaat acctgaaaca ggtacaaaag ggcatacatc tagagctcct
    10201 attcgaggat gagccccatg atgcgttctc atgtctatta gttcatatgc aacccgcgct
    10261 gctttcaaag cggcttcttt tacagcttcg ggaggcccaa ggaaggtata tactgtacga
    10321 tttgtgtccg cgctagggtc tatatcgagc agaaaaaccc cttgtacaga cctgatggca
    10381 tttgcgatag cttcgatctt ggttcgatct cttccttcag aaaaatttgg aacacattct
    10441 agcaagggct tctccatttt actcctccat tatatgaaat gtgttgagac ctaatacttg
    10501 gcgacgagcc tcttccaatg atctacggtt atttatattt tggaatacaa gtctaggatc
    10561 gcagagagaa attatctcat tctcatgaat gattagatgg ttttttattt taataaagtg
    10621 ccaaagggat aagcctgaac tgctttggat ataagaatat aattcaaaaa caagatgttt
    10681 gttatagaat gcaaaaaatg gctgaatgct tttttctgtc ttgcagagca caatatctac
    10741 atcctttgac atcgattgaa catagagata tcgaattaat tccaaagata cgaatggcat
    10801 atctactgcg caaaggcaaa gccagtcaaa cgaagcgtgg attaatgcag ttaatagccc
    10861 acatgcaggc ccgccttcct ttatatcgtc ttcaaactga gaagcaccaa ggctttcata
    10921 ggacttttga ggcttgccca cgatcagcag ctcttgggca atcttgctta gttgagaggc
    10981 tagcataaga cctatgggaa ttccatctat ttcaacctct tgtttgtcta ttccgccaaa
    11041 acgcctagca tgaccaccat tgagaatggc tgccgagaat ggcaatcttt gttcgccaga
    11101 gatgcctaag tcttcttgtt ctgcttttat atctgtcata ggatactatt atgacatgtc
    11161 ttgccaaatt catgcagata ctcgaatggg cagattggga tactccacca gatcagatga
    11221 actgctccct ctcttttcaa ttcataattc aaactattaa ggagctcatc cctgccattg
    11281 aatccgctca gctttccagc aaagaacttg attcttataa gattcacgac ctgctcgaca
    11341 aagcaattcg cctctatttg atgactccag caattgtaaa cgtgctgctg aactataaga
    11401 tctgtgtaga acatggcctt ccgcttcatc ctgctgtcta ttacgaactc aaggaagcgc
    11461 gcaagtaccg aatacgccat tcgctagccg aaattcagca agccaatgaa caatattggc
    11521 aatccataga ggttgctcgc ttgtgttatc aatgcttgcc tcaagccata ccagccatag
    11581 acaagcttta ttctaaaatt ccacctagca tcttttcatt tttatataca gcagtgcaag
    11641 atcgttacac atggcttggc tctcaacctt cactcctttc agagcttgct gcaaaaatat
    11701 caaaagaatt taagcctagc cttatcgtcg ctgccgccca tggttccatc atgcctgctc
    11761 ttatactttc agaactttta gaaatacctc tatacttcat tcggttttcg atgttcaaac
    11821 gccatgatga agaacctatc ataagcttat cggatcaagc atggcttttt gactatcgca
    11881 acaaggatgt tcttttatat gatgaagatg ttgcaggagg aaatacgctt gatttgttct
    11941 taaagagact atccccactt tttggagaag taaaaactgc ttgttcgatt cggcatgctg
    12001 gctcatccat acatacagat ttttcaggta gagtgtggtg ggattaggca tattattgat
    12061 cctaagcaat ttatttcata aatctgtata aattcccaga aaaactcgca ataagttgca
    12121 tattcattag ctctatgata tacttgtcta tagaaacagt tgccaaaaaa ccaatattgt
    12181 tctgttttca tgagcagtgg cgctgaggtt tacctccttt tcctcagcgc ctttcttttt
    12241 tcaattattt gatctcttag gcactatata atagtgacct aatcgcttga cttttatcga
    12301 tatatcagca taggttaaca taagtcatta aatcaatttt tcgcgcaggt tattagagga
    12361 gatattatgt ctcaaaaaac aaagcttaac tatgtcccat caatccgccg ttttccttca
    12421 tatctccata taatacgtgc ttttcagaga atcggcgaac cctatatttc cggcacttgg
    12481 atagcaaatg agctaaacct tgagccaata caggtcagaa aagatctctc cattaccggt
    12541 atagtaggga aacccaaaaa aggctatccg gtagatcagc taattgcagc catagagcac
    12601 tatctcgctt gggatattga acaaagagcg atgattgtag gcgcaggaaa cctgggatct
    12661 gctctaactg gctatcaaga attcagaaat catggccttc atatagtcgc agcattcgac
    12721 agcgaccctt tgaaggtagg caagactatt catcaaattc aagtgtacca aatgaatcag
    12781 ctaaaagaga ttgtagaaag gatgaagata actcttgcca tcttaactgt tccttcaccc
    12841 tacgcccagt caagctgcga tgcgatcatc gctgcaggca taaaggcaat atggaatttc
    12901 accaacgtga aactcaaagt gccagatgat gtgattgttc aaagagaaga tttatcttct
    12961 ggatatgcaa tactctcggt catgatgcgt acaaaagcta aactttccta aaatgggatt
    13021 ctatccctga gcttatgaag atatttttaa tttcaggccc acgcggttct ggaaagactt
    13081 cattctgcaa tgctctcgta aagactgcaa aagaaagagg catcctttgt agaggctgca
    13141 tagaaatttc gcagcgtgat gagaatggga taccttatcg tatcagtctt tgtgatgtat
    13201 caaatggaga gacttttctc gcagcttcgc gtcccatagg taaaagagat gttgcattcg
    13261 aattctctta tgatgccttc tcgtatataa gagaaagcta cattcaagct attaaattgg
    13321 atgctatttc gcaacatgct cactatgggc tagataaaaa aggggaaatt tgtttaatag
    13381 acgaaatcgg cccacttgag ctagagaata gtggtggcca tttatgcttt ttagaatttg
    13441 tgctccgcac aaaggaccca gacgcccttg ttttgactgt cagaacaagc ttgaaatatc
    13501 ctcttatttc tttccttgta aatcatgaaa tttcaataaa tcagatctgc aatctcgagc
    13561 ttgataaatt gacaatatct tccgcttgcc ttgatatttt ttcttttctt gagcaacgat
    13621 gattctttag ttgcacggtt taccttgaga tggtaaaatt taagtcacaa tagttttata
    13681 aaaattgaaa aagaggtttg aactatgcgc atgactacac gtggccttta cgctataaaa
    13741 gcaatcctct cgcttgtggc aatatcggaa gaacaaaaac ctgtctcttt aaataagatt
    13801 gcccaaattg aaaatctctc tcctgaattt cttcagcaga ttttctataa acttagaaaa
    13861 ggcggcatac ttaaatcctc gcgtgggcca ggaggaggct tttcgctgaa caaaaagcct
    13921 gaagagattt ccgcctatga tgtgctttac gcggtaggag agactctgga gatagtgcct
    13981 tgtgcctctc ggagaagtac aaagaaagcc tgctcaagat tcgatacttg ctatgtggga
    14041 cctttctgga aggaaatgga aaatataatt gtcgagtatg caaaatcaaa aagtttgaag
    14101 gacatttctt tagctagagc tcatcccgaa gtcatatcgg attaaaatca gacttttaat
    14161 atgtgtcgta ggaaatataa aagcttggga ttttcagatt tcaactaaat gctcttactt
    14221 gctatattgt ctttccaaaa tctactagct cagagtactg tacttcgctt atcttaccat
    14281 tctctttcag cgattcaaag agttcattga catgcatcaa tgcatgcagc cttacccctt
    14341 ctcgctccat atccaccctt ccctcgctgc ccctctctat cagaacaact aaatcgttta
    14401 tctctaaccc ttcagatcgc aatatttcta tagcctctag tttgcttgca cctgttgtta
    14461 ttagatcatc tagcaaaaga acgcgatctc ccttggcata tctgccttcg atatgctttc
    14521 ctgtaccatg ctccttgaaa ggcattctag gccagatcat aggcttgcca atttctagcg
    14581 aggccgcggt agcaagaggc aagcctgcaa caggaattcc agctataatg tcataatcac
    14641 aacatgatgc gagataggca taagctctgc ctgcaatcct catagcttct gggtctgcta
    14701 caagattccg aagatcgata tagaaagggc ttttcctgcc gctcttaagc gcaaattcgc
    14761 ccaatctgaa gcactcagta gagagcaaag catcgataaa atcgcgcttc aaggaaacga
    14821 cttcatctgt agcttttgta gctttctgag aaggttttga agctttagac aaaaaggagt
    14881 ctcttgccct cctaatttca tctctcagtt ctagagcagc attgtcgggc ctttgcgctt
    14941 ccgaaataga acgtgctgaa gcaacgagta tgcctttacc atcgctcctt gcgcctgcag
    15001 caaatgcaag atcagctctt cccccttggg caccaatacc ggggcttaga aaccaggatg
    15061 aaggtgcaac tcttctagcc ttctgaagcg cctctatatc attgcctgcc acaaccagtc
    15121 cgacacttat tggcaaagcg gagcatctct tggctatctc taagtagaga gggtctttgt
    15181 tcaccatgat atcttggagg aatcctgctc caggattact ggtccgacat aacacaaaaa
    15241 ctcctttatc gctccatcgc agaaatggct ctagagtatc gagacccaag tatggattca
    15301 atgtaaccgc atcggcttta agctctccaa aaattgcttg agcataagct tctgccgtat
    15361 tagcaatatc tcctctcttt gcatctatga taattggaac ttcttcggga atctgagcta
    15421 tagtcttata caaagcggat atgccggcat cacccagggc ctcgtagaat gcaatattgg
    15481 gcttgaaagc caacgcataa gatgtagtgc cttctatgat tctcaaattt tgctctagtg
    15541 ctagagctgc gcacgaagaa atccccctct tctctatctc agttctcgaa aacgcgggat
    15601 ccaagccaat gcaaagcaag gtgtccattt tatctgctct acgttcaata cgttcgaaaa
    15661 aatccatttc tgcttttcct ctataaaagt caataaagat aatatacaaa tgaatcgaaa
    15721 atatagtata atttttaaaa aagtaaagta gaaggaggcc tatcgtgaaa aaagcattct
    15781 tattattcgc tcttattctt gtatctcttc ctattttcgc gcagcaagga acaaatcctt
    15841 catttggctt tgcagcaaac ctggggacgg atctcttgcc tgatcccagt gacccgtcaa
    15901 agttcgaaag ctggtctaaa gtaggcctgc agcctgagtt caacattggg aaatttggca
    15961 ttggcctcga cctattgctg agattcaagc ttggtactgg aagcaataca aatctcgaaa
    16021 tatatgaacc tgactggatt cctcagcaag gccagaatat tttcgatgtc tatttaccta
    16081 agattctcta tatccgctat ggtcaacaat gggaagatcc attctatata aaaatgggct
    16141 caatctctga ttttagcctt ggcaatggtc tcatagtcga aaactattcc aacatgcgct
    16201 ttttgcctca acgacgcgta tttggaatgc aattgggagt cgatggaagc ctctttagct
    16261 ttccctattt gggattggaa gcactcactg gaaatatctc caagttcgat gtcataggag
    16321 gacgtgtcta tgcgagacca ctagccttta tgggcgaaag catttttgga aaactacagc
    16381 taggagtaac tagcgtgttc gatcgagatc cattattgta tactggttct ccttatcaga
    16441 ctctggcaac gaaactgatc tatgtagttg gagccgacat aaccttgcct cttatccaaa
    16501 gccctgcatt ttcgctcatc cccttcggag aaggagcata tgaaatgaat aaggctatgg
    16561 gagctatcgc gggcataagg gggcgcgcct ttggcctagt ctcctatcgc gctcagttta
    16621 gatatctcca ggagggattc atcccatcct atttcgatgc aaattatgat atctatagag
    16681 ccgatcgctt tgtatttatt gaaactaatc ccgcaaaaaa taattttatt cctggatggc
    16741 tcgcctctct aggctttgat ctcttcaaat ccagcctaag ctttggagta accctcgacg
    16801 ctccatttag agaactcccc acaactgcaa cggataaccc tgctgattat ccacatgtaa
    16861 agggtagcct aagtctagga cgcggcctca ttcctaatgt agacatcact gctagatatg
    16921 aaaaattctt ccttggtaag aagagcaacg atgtcatcaa agatctaatc gatctagagg
    16981 actcttatat tggcatgact gtaagctata aggcaggcac agctgtagta tctatggatt
    17041 atgcttatat ctggaatcca acaaagaata gtggaaaggg tgcctttgat gtggtttcta
    17101 gcctttctgt aggatttgaa ttataggctt ttatatagct taccgatgtc tcaggtagaa
    17161 ggcggtgttc ttaaaagagc gctgcctttg tttttttact aaaagccatt atcatacaaa
    17221 ccatgggaaa tatacgaatc ctgcctcctg aaacctctcg ccgtatagca gcaggagaag
    17281 taatagatcg gccatcttca gcgctgagag aaatcatcga caatgccata gatgccgatg
    17341 cgcatactat ttctgtctcg attgctcaag gaggaataga agagattact gttgccgatg
    17401 atggctgggg tatgtcgaaa gaagaccttt tgctaagtat tgctgagcac gcaacttcca
    17461 aaatatatgg accggacgat atcttgaaag caaagaccct tggattcagg ggagaagctc
    17521 ttgcatccat agctgcagta gcacgattgg aaatagtaag caagctcaga ggagctagcg
    17581 aaggatggag actgtctagc tctcccctaa aagagcctat catcgagcca tttgcctgca
    17641 aagaaggtac acgtttaact atgagggggc ttttcgaatc atatccggca agacgtcaat
    17701 ttctaaaaag accccagagc gaagccttcc tctgtcgttc cacctttatt gaacgtgcat
    17761 tgtctcatcc ggacatttgc tttcgctggg cagtgtcggg cgatatggat gtcatgatgc
    17821 catcttctct caaagatcgc attattcgat gctatactga gctttctcgt tttccattgt
    17881 tcgaagttat ggctgaaacc tctgagctaa aaatgcattg cgtctatgct gatgtatcaa
    17941 attataggag agaccgtaaa ttccttcagg tatttgtaaa taaaagaaga gttccggaat
    18001 ggaacctgct tagcttgatg gaatatgagt ttggaaaata cctgcctgga ggagcacatg
    18061 ctattgcctt tcttcttatc gaaatagatc cttcgcttgc cgatttcaac atacatccag
    18121 ctaaaaaaga agttcgccta aaaaaaccag cagaagtgcg gaccgtgctc catgaactct
    18181 tgtctcacga attggttcat agatatggaa gcttgactca agcttttaag gtagcaaatt
    18241 accctgaatt cgaggatttt gtcttttcaa ctaaagaaaa aacaagtgga ccaagcttgg
    18301 cggataaatc acatgttttg gcttcggagg gatgtggcaa gacaacgcaa agagttttgg
    18361 aaaatggttc atctaactta tactctatgc atcgaaacta ttcacaactt cccgatgatt
    18421 tctggcatag aataagagat gacaatctat ctgctcttcg atatatagga aaagggccag
    18481 gaccatttct cgtcttcgag ttgtctgatg cgctctttat actagatcag catgctgctc
    18541 atgagcgaat cctatataat aaaatcaaat ccaataaagg agagagtcag ttgctccttg
    18601 tcccttatct tctagagcct tatgaaaatg agcattactt ggaagaagca aaagataatt
    18661 tggaatttgt aggctacaga ataacaagag acaagaatac atggattgtt gatgctgttc
    18721 cagccattgc agcttctgca gctctcgaag cgcttgttga atggctttct cagccatcta
    18781 gtgaggcttc acctatcgat gctcttattg caagcttagc ttgcaaggct gcgataaaag
    18841 atggagacag acttgatgaa atctcagctc taaagcttat tcaagaatct ctagaattac
    18901 cggagcctcg ctgtcctcat ggacgtccaa tataccttgc tttctcaaga gaacaactat
    18961 attcgatgtt cgggagatct atcggatgaa aaaagccatc cgaatattat gtgtcgcaga
    19021 cgaagtggat ttacttgttt atagcacgca gatctgtgaa cgtttctcag atatcgatct
    19081 gatattgtcg gctggagatc tcccagatgg atatctcgaa tatatcgcaa gcatgctgaa
    19141 taagccactc gtctccgtag ctgggaacca tgataaatcc aatcaagata ttgagaatgg
    19201 taacatttat ggttcaagca atcaaagagt aaattttggt caaatcagct ttaaaatagc
    19261 gaaagaaaat ggtatatcta tacttggtct accaggctca attcgttaca ataaaggtca
    19321 gaatcaatat accgatgcat ggatgacatt tcgcataata ctgatgctcc caaggctatt
    19381 attgagacgc ctactatggg gaagatcggt agacatcatt cttgcacatt ctcctcctcg
    19441 cggtattcat gatggtggag atcccgccca taagggattc tccgcatatc gatggcttat
    19501 tcgatttgct aggccttatt attttatcca tgggcatgtg cacttatacg acatgcaagc
    19561 gcttcgcgaa atggattact ttaacaccaa agtcgtaaat gtatacgggc atcaaatcat
    19621 aactttacca aaggaggtta aaaatgaccg atgatttcca cgctcttgcc gaatccgatt
    19681 tttataaagc aagaagcaag gctattacta caacaatctt cggcatgcta agaccttcta
    19741 tgtccgatct gatgcctttc gatcaagcta aagagttgct taagcccaaa gctgagacat
    19801 atagagggat tactgcagtt cctatcgata gaatcgttgg aagcgaaggc cgctatagag
    19861 acttcaatcg tttctttttc ccaaagaaag aacatctaaa ggctcggtgg acaggaatag
    19921 atgaattgca atataaagac ataagccttc ctcctgtggt cttgtatgaa atgggtggag
    19981 tctactttgt ccgagatggc aatcataggg tatcggtagc taaagccaag aaacaggaat
    20041 tcatagatgc tgaggtcata tcgcttcaat ccgaaataca actcgatcca gaaatgacga
    20101 tcgaagatat aaagaaggct gtaatcgaat atgagaagag acgcttctat aaggaaacta
    20161 attatccaaa tattgttggg gcagatgatc tcaattttag cgaacccggc agattcgata
    20221 cgataaaaga acatgtctta gtacacaaat atttcttgaa ccagcatata tctgaagaga
    20281 ttccatttca tgtagctcta tattcctggc acgacaatgt ctatcagcca atctctcaag
    20341 caatagaagc agagaatata ctgtccctat ttcctggtag aaccgtatct gacttgtacc
    20401 ttttccttgt tgcccattgg gatgagctta aacgcaaatt aggccgctat gttgccatag
    20461 acgaagccgc tgagagtttt aaaaaccagg ctaaaagaaa tcgaaatagc tctttcgagc
    20521 atgcttgcag aatttttgga tccatttcaa taaaagtaga gaattttttt gcaaaattgt
    20581 aactttcaga acgatttcgt attataataa atgaagagca agtgaacgta gcttcttcat
    20641 gcagcctcca ttgtattagc cgtcggatat ccctccttat ccggcggcat ttttaataat
    20701 gaattgactt tttatgctga tttctgtatt gttactccgg ttttttggca aaaggagtca
    20761 ttatggcaca ggtttctaaa aatgctcgta caggctcttc aactcagcgt ttctattgtt
    20821 cttgcggtgg cgaaataaag atgaagacca tctttgaaaa cagcaaggtc aagactatag
    20881 ctgaatgcga aaaatgcaag agagttgaaa gacgcccaag cgacttcaaa tgatctttaa
    20941 gtgatccacg aagagaataa aaggccctag tatattgcta gggcttttta tttatatgca
    21001 ttgcttagca gaattctatt cgaaaatgta tcattgcatc cgaagttctt cgatttgttt
    21061 ttcgatcaaa tgacgcgcat cgttgagcat attttcgata tcaaatttac tgcgcggagg
    21121 ttcatatacc tttactattt caacaatata gggctctttg ccaaatctct taagaaaatc
    21181 catggcattt gctactcgcc agccgccctc tagagctatc gctgcaaccg ggagttttcc
    21241 accagactcg agcaattttc taacacctgc agtatgaaat tctcctacat gtccatttct
    21301 agacctcgtg ccttcaggga aaatcacagg gcaggctcct atctgtgtgc atatccttgc
    21361 cattttcgac acttctcgca ttgcatcaat aggagctcct tctcttttta taaggcaatg
    21421 ccctcctttt ctcagtgtaa agcttacaaa gggaatgcca tattgaagct ctctcttggc
    21481 aacaaagcgg ggtttccgtt ctattcctaa aaatgatatt agtataatga tgtctagcag
    21541 gctctggtga ttcgagatta caagacatcg aagaggaatt tctagtttct ctggattctg
    21601 cacactaatt ttgaatccat gaaatatcct gaatagccct agaatgatct ttgcaccaat
    21661 ttgctcatat ttagcacaaa gaatgaagcg cttatttctg tctgaaaata gacacctcag
    21721 cgcaagaata aattccagaa gaaacagcat agagattatg atcaaaacca ctatagcatt
    21781 gactaacatt atcataaatc tttaccttat tttgcctagg aatgcaatta cttcaaaaat
    21841 gtattgacat tgtcgcttgg tatctgctag cttaagcaca gaaattcgat tgaaccaaga
    21901 ttttcattaa acatagctat ctggaggaat tccattgagt acaagtaact tgtctgcaga
    21961 aaagcgccaa aggcaaaatg aaaagcgcag gcttgctaat aaaagtacaa aaacttccat
    22021 aaaatcggcg gctaggaaag tagtcattgc atcagaaaaa aaagatgcag cagcagcaaa
    22081 agaagctcta tcagagatga tcaagcggat tgattcagca gccagaaaag gaatagtaaa
    22141 gaaaaatact gcagccagga aaaaatcccg gatgcagcga cttgtaaata agatagatca
    22201 atagatcaga ttgagagtct agtaccttca gaaaggagca gagaacgata tggccgaaaa
    22261 attgacaaaa gccgaattga tagatgcgct gtatgattcg ctgtcgccga caagccggac
    22321 cacacggaag gaaatccatg agcttatcga cggtttattt gctgaaataa agggcgcaat
    22381 tcttgatggc aagatagtcg agttaagagg ctttggtact ttcgaggtaa aactcagaaa
    22441 aggcagatca aaggctagaa atccaaaaac gggcgaaatc gtttctgtca cagatcacgg
    22501 tgtagccacc tttcggcctg gcagagagct taagaaggct gcatgggaaa tggatatcga
    22561 acgcattccc aaatcattta gaaaaagagt ttgatcattg ctttttgcca aataaagaaa
    22621 gccattatta ataatggctt tccatgttga ttatgaaaaa gttgaatttg gaaccttcgg
    22681 aaccgtacgg aacacacatc gggccagctc ttatattgaa tgggatactg cgttcgagtc
    22741 agccccttga cttgcactgt gttttccatg gttcaataga agggacattc attaagattt
    22801 ctcgaaatgc caagatagat gttgatttgt taagggctag acaagttatt atagctggac
    22861 aattcaaagg atatatgagc gtttcggaag aaatagtaat tcttcctaat gctagtgtta
    22921 agagcgatat taaagtatca tgcctccata ttatagaagg tgcacaattt gaaggcaaaa
    22981 tcaggatggc tgagttttct ccagatgata aaagccaaac agaagataga agacattgac
    23041 atcgctacat tgtcgcacta tattgatatt atctattgca tattcgcggc gcaaatggcc
    23101 gcacttggtc caaaaaactt acctttcgat ttgactcaat gcgccgctaa agaatccata
    23161 taaaacaaat ggggagccat taagatgcct ggcaccgtct taatcgtata atatcatgga
    23221 gattctcaat ggcaattcat aatggaaata agacaacttt acccccagca aaatcttctg
    23281 ataatgagga gaataattca gcggtgccaa aagaggaaat agccatgaat caaagtcagc
    23341 acgaagagca acccatttta cagaatggat cacttttaga gggacagacc gaagatctcg
    23401 tgcaagacat acaacggtca ttgaacgatg tacatgttgc ctcacctttg aagaatcagc
    23461 aacagaaggt ccggaagcca gttactaaaa ttcgtttaaa aaagaagcct acgcaaaatg
    23521 tggacctcgt agaagaaagt caatcaaatg gatcgacaac aactgctgaa gcacttgaag
    23581 aacagcccaa tggatttgtt gttgcagcga tgcctctcga tgaccatcag catcaaaaat
    23641 tggttgagtt acccaagtca gagttggaaa gccgtgtgga acagagaatt gaatccagag
    23701 ctcgcctatt gatcaacgat ctttcaaaga tgggcataat agaattgaga gagctagggg
    23761 ctaaatatgg tataaatcat gatttgctca tcacgctaaa aaaacaagaa ctcattttca
    23821 atattctcaa agggcacacc gaacacggcg gcataatata tgcctatggc tcgcttgaaa
    23881 tccttccgga cggctatggc tttttgcgtt ctcctcaaaa ctcttatcta ccaggtacag
    23941 atgacattta catttcccct tcgcaaatca ggctctttaa ccttaagaca ggtgatactg
    24001 tctatggtca gatcaggagc cctaaggaag gtgagcgctt ttttgccatg cttcgtatcg
    24061 aacagatcaa ttttgacgat ccttcggtag ctcagaatag aattcccttt gaggacctta
    24121 cccctctcta ccccacgcaa aaattggatc tagaaaccag cacagaagaa atatctacta
    24181 gaatcatcaa tcttttctgt ccgataggaa agggccagcg cggactcatt gtatccccac
    24241 cccgtactgg aaaaaccatt cttttacaaa aaatagccaa tgccataaca gccaatcatc
    24301 ccgaagtcta tctcattgtt ctcctcatcg acgagcgacc tgaagaggtc acagatatgg
    24361 agcgaacagt aaaggctgaa gtcatctctt caacattcga cgaacaggcc actcgtcatg
    24421 tccaagtggc tgagatggta ctagagaaag cgaagcgcct tgttgagcac aaacgagacg
    24481 ttgtaattct gctcgattcg atcacaagac tagcacgagc ttacaaccaa accgtcccca
    24541 cttcgggcaa gatcctttct ggaggtgtag attcgaatgc tctacacaag cccaagcgat
    24601 ttttcggagc ggcgcgcaat atagaacagg gaggttcatt gactatcatt gctaccgccc
    24661 ttatcgatac cggttcacgc atggatgaag taatctttga ggagttcaag ggtacaggca
    24721 acatggagat caacctcgat agacgcttgt ccgatcgccg tctctaccct gctatcaaca
    24781 ttaagaaatc cggaacaaga aaagaagagc ttctaatctc agaagaagag cttcaaaaga
    24841 tttgggttct gcgtaaagta ataaatccga tggatgatat cgaaataatt gaactgctca
    24901 ttgaccgcat gatgaagact aagaacaatg atgcctttct caaatcaatg aatactcctt
    24961 attcgagcat ggaataagca tattgactag agctggtcga ttttatatat tgaagcgccg
    25021 tgtcttgcta gttttccaat agaaatacta ctaagaccct atggaggaat aaatgaaaaa
    25081 gggaatacac ccaaaatatg agctcacaac aataacttgt gcatgcggaa atgtcattga
    25141 gacccgctca acagctaaga atatccaagt agaaatctgt tctgcctgtc atccattctt
    25201 cactggcaaa cagaaacttg tcgatactgc agggcgcata gagcgcttta ataagaaata
    25261 tggaataaag gctaacgata acgattaatt g
//
LOCUS       DAOSOB010000025        24178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_2096_length_24178_cov_73.943208, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000025 DAOSOB010000000
VERSION     DAOSOB010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 24178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24178
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2096_length_24178_cov_73.943208"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="PLS88_04135"
     CDS             <1..1667
                     /locus_tag="PLS88_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU06571.1"
                     /translation="RGRVFSDRWPTIPQLFDITTDRFPDRACFTIYSPQRMSTNYRET
                     HEIILKVASYLTSIGLGPGSHIAVTGKNSPEWGIAYIAVLYTGAVVVPIDYQLSTQDI
                     ITLMHAGDVTAAFVDEEKYEHIKKDFAGIPCFGLARGVESFIYDLEPPAGFVFTPPQV
                     PVQSTAAILFTSGTTGDPKGVMLSHENLVSDCFLAQSNLNISEQDIFYALLPIHHSYT
                     MLAVFIESFSVGAETVFGKRMVVKQILSDLKEAKITMFLGVPMLFNKLLTGILKGVKE
                     KGALAYGLIRTLMAISGFIKKTFGVNPGRAFFGSILEKASLSSIRICISGGGPLDPKV
                     FRQYNQLGIDFVQGYGLTETSPILTLNPVEHYKETSVGKVLPEVEMIIDNPNEDGIGE
                     IVVRGPMVMQGYYKMPEETAEVLSPDGWLRTGDVGKLDDENYLYLAGRIKNVIVTEGG
                     KNVYPEEIENEFQLYDEIDQILVRGYIPEGKTVAEEIESLIYPNQDWISQNHLSPEQV
                     EERIHDIVTEVNQRLRSYEKITRIHILKEPLEMTTTQKLKRHIAKG"
     gene            1789..2562
                     /locus_tag="PLS88_04140"
     CDS             1789..2562
                     /locus_tag="PLS88_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU06572.1"
                     /translation="MYCDAHLHLVDLDQHDPGFAYRALSYDWRGAVVSHDPEEFLQSE
                     ALRVKLPFTIAGFGIHPQEPDMQHAEFLSMLCAQRKIDFIGEAGFDFFGDKPLRSRSA
                     EAMQLQLEAFLFQVKLADHARLPLVIHIRKATDVIMANGRELRRLPSIIFHCWPGRLE
                     EAQMILKKGINAYFSFGTPLLRDSRHAIESLKSLPVSRILSETDAPWQPPHGAEYTSI
                     NHIVDVVEKMASVLNMNQEELKHKLALNFTDAYLIEGPK"
     gene            2599..3351
                     /locus_tag="PLS88_04145"
     CDS             2599..3351
                     /locus_tag="PLS88_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOU06573.1"
                     /translation="MVEPIEKIGPSERTEGSFLDRTRIIAGDDGLARLAYARVAVYGL
                     GGVGAACALDLVRAGVGYLHVIDYDIVEYSNLNRLAFGYNRYAGIAKTKAFTDAAKEI
                     NPAIQIVSENIFFSAESAAQIIARECCFHADCIDSLSPKVNLIASLYLERLIFISSMG
                     TAGRIMPESLRLGTMQEVKGCPLARALRQRLGKMAIPFDFPVVWSDEPPIKPICLEGK
                     RRIQGSMPFVPQTAGHIMASWIVRTILKKQSS"
     gene            complement(3382..4647)
                     /locus_tag="PLS88_04150"
     CDS             complement(3382..4647)
                     /locus_tag="PLS88_04150"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU06574.1"
                     /translation="MQEAAFYDIHCHLLNLSHPAFVSIIESMRHRPREVIYSQIASFD
                     YLASSLLKRGGEKLRNLLAVMDNDCADILFLLEDDLSGQFKEPKGTPSFMKPPPLRNG
                     ALHVADQQFSSFVLTPLIMDFLAPSSFSPQTYYNRPPQKWIEAQIIDVLFAICTYREK
                     RPSGFLRVYPFLGINTGHYTAEELSSFLDQWFNDYTKNEDDIEKRFFSFSYDGNTAPQ
                     SYGIFSGIKLYPPLGFDPWPEDQAEREKVELLYSFAETKGIPITTHCDDQGYRIISLE
                     ESLKFTAPSRYKPVLQKYPRLTLNFAHMGKRHIRTIGGKEQADWRKEIFDFIIEYPNV
                     YTDFSFTAGEPSFYEELDSTLGRLKKPEKEKIIKRILFGSDFMVNLFKVRSYRDYLEY
                     FSESILSTELKRLFCSDNPQRFLFGADAE"
     gene            complement(4673..5587)
                     /locus_tag="PLS88_04155"
     CDS             complement(4673..5587)
                     /locus_tag="PLS88_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU06575.1"
                     /translation="MNKMGGILAGIGYSTIFGFSFLMTKEALEVLNPMELLAMRFVIA
                     ALLMSILAVAGMIKVNFKGKPIRLLALMCLFQPIAYFTFETYGLANSATNVAGIILGA
                     LPAGVAIMEALLLKERLNTLQSIGLAMSIVGVIFVAVFGQGYNNETKPIGVLFLTGSM
                     LSAVFFNITSKKAAESFSPVERTFAMMWSAAICFSIPALWENIKGKGNLGYLFGEWNR
                     TAGVWGGIVYLGILSSVGAFFLINYSLSQLKASQSAVFTNLVTVITVLAGVLIRKEQF
                     NWSQGLSVILIILGIAVANSSFRDKAKS"
     gene            5630..6067
                     /locus_tag="PLS88_04160"
     CDS             5630..6067
                     /locus_tag="PLS88_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06576.1"
                     /translation="MMQTDVEAFWTSFEQEIGDKIISKTMGQHFSSKKSQGEWGLLVL
                     TTLSIRFRPTPGENWFQSLFRMATPKVPKEPASDIVLPLALITSIELPKKHFFDFLFS
                     PPFTIFTLRYRLGDEEHSVLLGADPKSELFTKLMSTIPVMKGK"
     gene            6064..7161
                     /locus_tag="PLS88_04165"
     CDS             6064..7161
                     /locus_tag="PLS88_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU06577.1"
                     /translation="MTNIYTQLEKANLLFFEYGITALDSYFDEKPRPIKFLAVQCSLI
                     ELARAFASLEYPGLPYADASLYDEARFICSEDALEEDLGNPWMNFRRNPSNGVFYDKG
                     NIYNSLKLRSIPTLFSESPELERSLFDFTLMATFSEIPPSLPKSLSVPTSPSILLQRD
                     LLVGILSGPYPASGFEVLKQSGFIAREWPEIALLDNVDHSKDFHPEGNGWNHTMQTFS
                     YRKNRDLILSLALLLHDIGKWESKSYEGHRFDRHAELGARRAEKFLSRLGFSEEIQKD
                     VKFLIRYHMMPAALPTLPIQKTEELIRDPRFPLLLELFRCDEFSSFKDAERYYDACNT
                     YRSICRNLRNPYRKADGSKVYASRRSDSHFS"
     gene            complement(7156..8370)
                     /locus_tag="PLS88_04170"
     CDS             complement(7156..8370)
                     /locus_tag="PLS88_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU06578.1"
                     /translation="MKRKLFVPIMITLVSLLSIGLLIYSIQRHIAGRSFESVNNAISS
                     LIEIQASALYNGCFSRNQTYYLVIEEKLADANILVQKATDSFPLIDLVEIVEGRPPAD
                     SFDVILDGELLRIECPIRNGDDETTVPNMVGVAIIQAQRLLDTVSAKDFRIDLVHGRK
                     TTYGIPISANFTSHSLLDSIIIVLLIGFIAVSIFQHYYRKANTFLDTRGLESIIYLFE
                     QTERISASHSRNVAMLALFVGKNLGFRGKKLKDLYIAALLHDIGKIGIPNNILTKNGK
                     LDSCEFVQMKQHPAISARILRNFKEYERLSPIVLYHHEREDGSGYPEGLTGKDIPLES
                     KIIAVVDVFEALVGERPYRDPICPLSAFQKMKSMPLDQKIVSLLIDHYGELVSFRPPR
                     WALSYSPWLLAS"
     gene            complement(8410..8637)
                     /locus_tag="PLS88_04175"
     CDS             complement(8410..8637)
                     /locus_tag="PLS88_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06579.1"
                     /translation="MKTVFISAFLEDSIVVKTMLESAGIPAEVLSDQMLDINPFYSIE
                     PKGAKVIVPDSAEADAKAIVADYKEHKKSNK"
     gene            complement(8634..10046)
                     /locus_tag="PLS88_04180"
     CDS             complement(8634..10046)
                     /locus_tag="PLS88_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOU06580.1"
                     /translation="MQEVYKKRRQAFLKAIRDRGITRGTVLLLANSHCPRNYPDNCYP
                     FRQDSSWLYLIGLNIPDLACTLDIESEESCLYAEELGLEEIIWTGELPSPVDQAYSVG
                     IEKVLPTSYLSNNVEQTLKLREPVFILPPYQDRKIAQLSEIFGTARSSLEELIYRYID
                     PRVIMALVSVREIKEPEELAEIERAVSITKNIHEELLHQLKAGWTEKDAAGFVLSRAS
                     AQGCELSFATVATCRASVLHNSPTEHIATAHDTFLLDAGVETPLGYAGDLTTTFPVGP
                     SFSHQSKEIYQVLCESFRTATNALAPGVRFLDVHKKASLTITKGLISLGLMKGDPVEA
                     VESGAHALFFPHGLGHQIGLDVHDMEGLGEDYVGYGEELKRSEQFGLRSLRLAKTLKP
                     GMVHSVEPGIYFIPQLVQKWRTERICENFLNYDIIEKWMPVGGMRIEEDWCIIDKGAR
                     RLGPAFDKSIEAIENVRANR"
     gene            10205..10564
                     /locus_tag="PLS88_04185"
     CDS             10205..10564
                     /locus_tag="PLS88_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU06581.1"
                     /translation="MDYVRYCFASNLRLRRVILQMSQEELAHLANLSPGFIANIETGR
                     NFPSSKGILKISSALKVEPWKLFLDPQKQDMFFTRDEVSHWLEDSRKRLFGDFSQGEY
                     HTENDSQIDKDDITPQD"
     gene            10596..12050
                     /locus_tag="PLS88_04190"
     CDS             10596..12050
                     /locus_tag="PLS88_04190"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU06582.1"
                     /translation="MEHNSRSLWIAILFLVLAKGFSASAQCQIKTLANGMTVCLNPIE
                     YEEDISIILAYRAGANSQTASTAGLFKLLEYVLFNGPAANPGIPEPAAAIDVLEAENI
                     IGGVGIDRFEFGIMFKKENLIPALDTFLYLFSLERREFILAQIDGIELAKRSVLALIQ
                     NETSDNDLLSSMAINNKLFSKAPFRTDILGAENVLEKADAAQLNNIASSWLVPNNACI
                     SISGAFDSSQVMNLIEERFSLLPKAKNPWPANLASFPKPGVTKPIFLVLPDSSIPADH
                     IQIEMRYRGPDPKDTTSYFAALMLEQLVNEPTSRFQTAMRKAMPKGSEPENLRIDYVP
                     AANSSWIGVQSIMPAGKKPADTVFAFKEAVRGTELYMIKANPSYFSATDYEQARQALL
                     EEIMAIQGDPLRSACHMASLWSWGIPSFIFQEGDIIMKTGQKEVSQFVDIYVQKNLEV
                     VMVRIDPNLYEANKKSFSSYGFETVSAKNAFWWR"
     gene            complement(12062..14299)
                     /locus_tag="PLS88_04195"
     CDS             complement(12062..14299)
                     /locus_tag="PLS88_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOU06583.1"
                     /translation="MDELVQDFSSKIDYRFNDEEKNLLLKALDHSRHTHGERKRASGE
                     PAYYHDIRVAEILLDLGMDLESILAGLLHDTIEERISGFAHEANSDSKIEGNPASKDK
                     SAFEDSQIQSRGILQAPRGERALKKKGSTTLNSLLAAPDLFIQEQFGPQTAMIVAGVN
                     RLSSVKAKNKTVHAAETMRKMLFALTSDIRVILVKLADKLDSMRTLKYLPEDRQKEIA
                     TECIDIYAPLADRLGISWLKDELEDLSLKSLNREVFDQIKDIVNSRKNERQEFLKKVT
                     DKILSAAAKEGIDVEVSARAKHFYSIYQKMRRRGKGAEELYDLLGIRIICNSINDCYS
                     LLGLVHRLWKPIDGRFKDYIAMPKANGYQSLHTTVIAYGGQLLEIQIRTREMHLVAEY
                     GVASHWLYKKGTRAEIPKLEDLPIINKLRQWNQFIAEGDSYLEEIKQEFLGDSIFVFT
                     PKGDIIELPSGSTPIDFAFAIHSDVGAHCLGAKINGAIAPLDSELRNTQIVEIITSPN
                     AKPNLNWLKIARTSKARSKIRQLLVQTGQAVAIDKHIVAARKSEKATQETVQARTHGA
                     ADRSQTEARRSESFPSVVEFHSLKPGLVLRSEKAGVSVGGMRNMMIRIAGCCKPVTGD
                     RIIGYVSRGRGIIVHREDCRSIASISDFEERRIDVKWEDEAINTTARYRVTTKKSFDI
                     FSEIESTVRKCSGRLKEGKLGERGDGTLSGFFILEVDSHEDLKRVAKALRTLPSILSI
                     EEEPI"
     gene            complement(14292..15161)
                     /gene="prmC"
                     /locus_tag="PLS88_04200"
     CDS             complement(14292..15161)
                     /gene="prmC"
                     /locus_tag="PLS88_04200"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOU06584.1"
                     /translation="MTYRELIFNESCSFSESDTPFLDALLLLAHSLGISKEKLFSMLP
                     ECADSIPQSFFDMTERRRNGESIAHIRGFREFYGREFLVNDDVLSPRQDTEILVEAAL
                     ESSKLFEPVADIEEDLEAGREISVLDLCTGCGAVAISLAAEKPWFKLTASDISSAALD
                     IAKKNAQRLLTATSLIFVESDLFSAIDGKFEIITANPPYIPHCEAERFISKGWKDPLI
                     SLDGGIDGMKHIRKIIMEGYEYLSHNGVLLLEMDPYQTSESLALFKKMGYIDLRTWKD
                     LSGSIRVVGGRHG"
     gene            complement(15167..16246)
                     /gene="prfA"
                     /locus_tag="PLS88_04205"
     CDS             complement(15167..16246)
                     /gene="prfA"
                     /locus_tag="PLS88_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOU06585.1"
                     /translation="MTEYIEHMEQLRERLAEIDDLVSKPDVLRDQKNYRDLMKERSRI
                     SEILDAFDETQRLCSSMHETEELIQETKDEELRELAEEELDQLRNQSALAEERLEALL
                     APRDPMADKSVIMEIRAGTGGEEAALFAADLYRMYSRYADSREWNVEIMSSNNTELGG
                     IKEIIFSISGDSVYERLRLESGVHRVQRVPVTEGSGRIHTSTVTVAVLPEMEETEIEI
                     RPEDLRIDVMRSSGPGGQSVNTTDSAVRITHIPSGIVVHCQDEKSQIKNKAKAMRILR
                     ARLFEIEDDKRQAERSAERRNQVGTGDRSERIRTYNFPQNRLTDHRIDLTLYKLDLIM
                     EGDLDELLDALIAWDREQALIAEER"
     gene            16325..17605
                     /gene="serS"
                     /locus_tag="PLS88_04210"
     CDS             16325..17605
                     /gene="serS"
                     /locus_tag="PLS88_04210"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOU06586.1"
                     /translation="MLDIRFIKDNLEAVRQNIRDRYMDADVDLLISLYDKKNALLREL
                     EESRRCRNENAQAMKSNLSKEERSIRIEEGKRLKETIARLEAELQEVEAAFAEELAKI
                     PNMAHPQAPRGKEDKDNLEVKRVGSPTQFSFPPKDHVELGQSLDILDLDTATKVSGTK
                     FYYLKNEGTILELALVRYALDMLSKHGFTLMTTPDIAKTEILEGIGFNPRGAESNVYT
                     LEGEGTCLIGTAEITLGGYHSDSILEKDQLPIRYAGLSHCFRREAGAAGQFSKGLYRV
                     HQFTKVEMFVYCLPEESEAIHEELRSIEEEIFSSLDIPFRVVETCTGDLGAPAYRKWD
                     LEAWMPGRNNGEWGEITSTSNCTDYQARRLNVRYRDKDGKNRFVHMLNGTAIACSRAI
                     IAILENHQQADGSVRIPKALVPYCGFDLIQPHHV"
     gene            complement(17677..18156)
                     /locus_tag="PLS88_04215"
     CDS             complement(17677..18156)
                     /locus_tag="PLS88_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06587.1"
                     /translation="MNSEPKSNKRPNTSLRHGKPEKKIGEQHFRKRRSAHAAAQKMVD
                     HAQENNVRAGKPSAESPRQEPKICRVCGKPIFDFAGAIASREDGEPIHFDCAIEILSK
                     EENLAPDEKLIYIGSGFFAVCVQSPSGKLEIRRRIRWENPGTQQPWRKPMIFSPNLP"
     gene            complement(18153..18374)
                     /locus_tag="PLS88_04220"
     CDS             complement(18153..18374)
                     /locus_tag="PLS88_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06588.1"
                     /translation="MKQKKVSRDIPAWAGSTPDDLLYSDYDITEEMKATNWKRLYEIK
                     HKLVDIDYLDGAIASIAEMVSNTIRNKSK"
     gene            complement(18352..18756)
                     /locus_tag="PLS88_04225"
     CDS             complement(18352..18756)
                     /locus_tag="PLS88_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOU06589.1"
                     /translation="MVLMLKTDSEKKKRRGKEEEIIAGQYLESIGWIILARNFRSRRG
                     EIDIVAVRENILSFVEVKSANRITKRDLEHVIGIKKRTSIIETSKLFLVINRKYKRYH
                     IRFDVMLIQENACLRHIEGAFSEDGDEAKKGF"
     gene            complement(18905..19276)
                     /gene="rplS"
                     /locus_tag="PLS88_04230"
     CDS             complement(18905..19276)
                     /gene="rplS"
                     /locus_tag="PLS88_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOU06590.1"
                     /translation="MDLIQQIQAEQIRTDLSDFRVGDTVRVSFKIVEGKTERVQAYEG
                     LVIAIKNAGIGKTFTVRKNSYGVGVERVFPFNSPRVEKVEIIKAGKVRRAKLYYIRSK
                     VGKKSKVKTLVGGRKSQKQEA"
     gene            complement(19224..20003)
                     /gene="trmD"
                     /locus_tag="PLS88_04235"
     CDS             complement(19224..20003)
                     /gene="trmD"
                     /locus_tag="PLS88_04235"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOU06591.1"
                     /translation="MRIKLLSLFPEILEAYFSASIMKRAVAKGLISYELVNIRDYAFD
                     RHRKCDDEVFGGGPGMLMKPEPLDRALAAAGSPELRTIYVTPSGRLFNQAMALDFARE
                     ENLIFLCGRYEGIDQRIIDTRITDVVSIGDYVLSSGEIAAMVVIDAVYRLIPGVISGD
                     SLSEESFSKGLLEYPQYTRPAKYDNLFVPEVLVSGDHAAIERWRLKASLIKTLVYRPE
                     LLQNIDLTSELGRLLKEVIDEGGGHGPHTADSSGTDSNRPV"
     gene            complement(20000..20527)
                     /gene="rimM"
                     /locus_tag="PLS88_04240"
     CDS             complement(20000..20527)
                     /gene="rimM"
                     /locus_tag="PLS88_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOU06592.1"
                     /translation="MHDRQKPEYLAVARLGAVRGLAGELRLFSYSGEYSHIEGAQEFF
                     LGGREGLKDAKPIRVLRLLKASWGVSIVFEGFETPEKARQLVGRDIFLPRSEASPKKS
                     GEYYIADLVGMTVIADGANVGMITAVIEGAESDLLEIQRDLEERILVPFRKEFVGKID
                     EEKGELEIISPWILE"
     gene            complement(20543..20776)
                     /locus_tag="PLS88_04245"
     CDS             complement(20543..20776)
                     /locus_tag="PLS88_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOU06593.1"
                     /translation="MERDLVEYIVKSLVDHPESVDIKVVEGEKSTILELRVAESDIGK
                     VIGKNGRIARAIRTILSASASSKSKRVILEILD"
     gene            complement(20791..21042)
                     /gene="rpsP"
                     /locus_tag="PLS88_04250"
     CDS             complement(20791..21042)
                     /gene="rpsP"
                     /locus_tag="PLS88_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOU06594.1"
                     /translation="MSVRIRLKRFGAKARPYYRIVVMDSRSARDSKTIDEIGYYHPIE
                     AEDKQISINEAKVREWLAKGAQPSDTVRSLLNKKGIQLK"
     gene            21244..23193
                     /locus_tag="PLS88_04255"
     CDS             21244..23193
                     /locus_tag="PLS88_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU06595.1"
                     /translation="MANTIPQMYIEIAQRQPNVPVQLSKDAQGDFQPITYGELLKEIS
                     IFALGLGDFGIKRGDNIGFISDNRKEWLLADLAMLGIGAVDVPRGCDATAQEIAYILS
                     WSECSTTILENERQLKKILDVKQGRISFNSMSLIEAPYANLRSIILIDPPSSQIAEEA
                     RQAGLTISIFSEIMEKGLSLYKNDPDFYQKEAQKGTRADLATIIYTSGTTGEPKGVML
                     SHGNFLHQTEYFPYLLEVNSGEVFLSVLPIWHSFERAVQYILLQAGCTIAYSKPIGSI
                     LMEDMEVVQPHWFSTVPRIWESVKDGVDKTIRQSGGLTKSMFSMFVGIGKAHAYFKNM
                     LYDQLPKFSPRSRFVDISISIIPFALLTPLRALGDALVFKKIKEKLGKRFIAGVSGGG
                     ALPPSVDHFFEAIGILILEGYGLTETAPVISVRLRNHPVTGTVGPVHRGTEVRIVDER
                     GNVLPAGQKGVIHVRGPQVMLGYYKRPDLTEKVLSKDGWLDTGDLGMFTIHGELKIIG
                     RVKDTIVLRGGENIEPLPIEQRLCESPYIQQAAVVGQDQRYLAALIVPNQEVLTAWAE
                     ENDIPFNDYPSLLEQPEVKELINSEINSYISIKNGFKPFERIYHFALLPSPFEEGREL
                     SAKLDLKRHVINELYADQIRQLFEE"
     gene            complement(23272..>24178)
                     /locus_tag="PLS88_04260"
     CDS             complement(23272..>24178)
                     /locus_tag="PLS88_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOU06596.1"
                     /translation="HGLRTRRHNLMRTEGIDIDFRFLEDIQDVEGIRRIVIRVSEGNI
                     IVTRSEDPRLRYFVELQGVPGEIDTWSCRFRRAESIAIIEAIGNDFVRIGKCNIFIPS
                     HISDIEVHSSRGSIEIHDIPANVLAITDRGDISISNAKFVEVSSVNGNIIVDNSEGCS
                     ARSINGTIRCSRIAGSVQVETQSSFVLIDQVEKNVAAVCDQGKIITRRVKGRVRLISN
                     KGDIELEVSGAFGGGEVQTYSGDISIQLEYSSIEFRAETLSGRIEASFPVSASGIGPQ
                     RCTFRTGEGSRRLYVKSILGDIEIS"
BASE COUNT         6624 a         5618 c         5115 g         6821 t
ORIGIN      
        1 atcgaggtcg cgttttttcg gacagatggc ctactattcc acaactattc gacattacaa
       61 cagatcgctt ccccgataga gcatgcttta cgatatacag ccctcagaga atgagcacaa
      121 attatagaga aacgcatgaa atcattttga aggttgcttc atatctcact tcgattggcc
      181 taggaccagg tagtcatata gcggtgacag gcaagaactc tccagaatgg ggcatagctt
      241 atatcgcagt tctctacaca ggtgcagtcg ttgttcccat agactatcag ctgtcgacac
      301 aagatataat aacactaatg catgctggcg atgtaacggc agccttcgtc gacgaagaga
      361 aatacgagca tatcaagaaa gattttgcag gaatcccctg ttttggcctt gccagaggtg
      421 tggaaagctt tatctatgat ttggagccac cggcgggttt cgtttttacc cctcctcaag
      481 ttccggtaca aagcaccgca gccatacttt tcacctcagg cacaacaggc gatcccaagg
      541 gagtcatgct tagccacgag aaccttgtct ctgactgttt cttggctcaa tcaaacctga
      601 atatctccga acaagatata ttctacgcac ttttaccaat ccaccattcg tacacgatgc
      661 tggcagtctt tatcgagtct ttctcggtcg gagcggagac tgtctttggt aagcgcatgg
      721 ttgtcaaaca aattctctcg gatctcaaag aagccaagat caccatgttc ctcggagtgc
      781 caatgctctt caataagctc ctaacaggta ttctgaaggg agtcaaggaa aaaggtgcac
      841 ttgcctatgg cttaattagg actttgatgg caatttcggg ctttatcaag aagactttcg
      901 gtgtaaatcc tggaagagct ttctttggtt caatcctcga aaaagcaagc ctatcttcga
      961 ttcgcatctg tatttcggga ggcggcccac tagatccaaa ggtattcagg cagtacaacc
     1021 agctcggcat agactttgtc cagggctatg gtcttaccga gacctcgcca atactcactc
     1081 tcaatccagt cgaacattac aaagagacat cggtcgggaa agtgcttcca gaggtagaga
     1141 tgattataga caaccccaac gaggatggta ttggcgaaat cgtggtccgc ggtccgatgg
     1201 taatgcaggg gtattacaag atgccagaag aaacggcaga agtcctttcg ccagatggtt
     1261 ggctgcgaac tggcgatgtc gggaaactag acgatgagaa ctatctatac cttgcgggca
     1321 gaataaagaa tgtaattgtc accgaaggtg gcaagaacgt ctacccggag gaaatcgaaa
     1381 acgagttcca actctatgac gaaatcgacc aaatccttgt acgaggctat atacctgaag
     1441 gcaagactgt ggctgaagaa atcgaaagcc tcatataccc aaatcaagat tggatttcgc
     1501 agaatcacct ttcgcctgaa caagtggaag agcggattca tgacatcgta actgaagtaa
     1561 atcaaaggct gcgctcttat gagaagatta cgcgaattca tattctcaaa gagccactcg
     1621 agatgaccac tacacagaag ctcaaacgac acatcgcaaa gggataagaa acttgcttta
     1681 atagctggaa tgttgctgtc tcgattattc gtcaattata ttgagctcac tctataagaa
     1741 gatgttgtaa acattgacca gaatgcttca tagttgcaca atgtccttat gtattgcgat
     1801 gctcaccttc accttgtaga tcttgaccag catgatccag gctttgcgta tagagctctg
     1861 tcatatgact ggcgaggtgc ggttgtatct catgatcctg aagaattttt acaaagcgaa
     1921 gccttgcgtg ttaagctgcc ttttacgatt gccggctttg gaattcatcc tcaagaacca
     1981 gatatgcagc atgccgagtt tctcagcatg ctctgtgcgc aaagaaaaat agattttata
     2041 ggagaagcag gcttcgattt ctttggcgac aagccactgc ggtcgagaag cgctgaagcc
     2101 atgcaattgc agctcgaagc ttttcttttt caggtaaagc tggcagatca tgccagactt
     2161 ccgcttgtaa ttcacattag aaaggcgaca gatgtcatta tggctaatgg ccgcgagctt
     2221 aggcgcttgc cttcgatcat tttccattgc tggccaggaa ggctcgaaga agctcaaatg
     2281 attttgaaaa aaggaataaa cgcctatttt tcctttggta cgccattatt gagggattcg
     2341 aggcatgcaa tagagtcctt gaagagtctt cctgtttcga ggattctttc tgagacagat
     2401 gctccctggc aaccgcctca tggagctgaa tacacaagta ttaatcatat tgtcgatgtc
     2461 gtcgaaaaaa tggcctctgt actcaacatg aaccaggaag agctaaaaca taaactcgct
     2521 ctgaatttta cagacgcata tcttatagaa ggccccaaat agaagaccca aaaaatctaa
     2581 cagaatggag caagatatat ggtagagccc atagaaaaaa tcggtccatc tgaaagaacc
     2641 gaaggatcat ttcttgatag gacgagaata atcgcaggcg atgatggact tgctagactc
     2701 gcctatgcaa gggtggcggt ctatgggctt ggcggtgtcg gagctgcctg tgcactggat
     2761 ctcgttcgcg ctggagttgg ttatctccat gtcatagatt atgacattgt agagtattct
     2821 aacctcaata ggctcgcctt tgggtataat agatatgctg ggatagccaa gacaaaagca
     2881 ttcaccgatg cggccaaaga aatcaaccct gcaatccaaa ttgtaagcga aaatatattt
     2941 ttctcagccg aatctgcagc gcaaataatt gcacgcgagt gctgttttca cgcagattgc
     3001 attgattcgc ttagccccaa ggtgaatctt atcgcctcgc tatacctcga gcgcttaatt
     3061 ttcatatcga gcatgggaac cgcaggccga ataatgcccg agtcgcttag gcttggcaca
     3121 atgcaagagg tgaaaggctg ccctcttgca cgcgcgctgc gtcagcgact cggcaaaatg
     3181 gcgattcctt tcgattttcc tgtagtatgg tcggacgaac ctcctataaa gccaatctgt
     3241 ttagaaggga agcgtcgcat tcagggttca atgccttttg tgccccagac tgctggccat
     3301 atcatggctt cttggattgt caggacaatc ttgaagaaac agtcttcctg actggttctt
     3361 ttcgagctct gcttgctttg tctattcagc atcagcaccg aacagaaatc tttgtggatt
     3421 gtcagaacag aaaagacgct tcagctctgt acttagaata ctctcggaaa aatactctag
     3481 ataatcccta tatgaacgaa ccttgaaaag atttaccatg aaatcagagc caaatagaat
     3541 gcgctttatt atcttttcct tttcaggttt ttttaagcga cctagcgtag agtctagctc
     3601 ctcatagaac gaaggttctc ctgcggtgaa tgaaaaatca gtatatacat tggggtattc
     3661 gataataaag tcgaagattt ccttacgcca atcagcctgt tctttgccgc ctatagttcg
     3721 aatatggcgc ttgcccatgt gggcaaaatt caaggttagc ctcggatatt tttgtagcac
     3781 aggcttatat ctagatggag cagtgaattt caatgattct tccaaagaga tgatgcgata
     3841 accctgatcg tcacagtgcg ttgtaatcgg aatgcctttg gtctcggcaa agctatatag
     3901 caattcgact ttttctcttt ccgcttgatc ttcgggccag gggtcaaagc ccaatggcgg
     3961 atagagcttt atcccggaaa agataccata actctgagga gcagtatttc cgtcgtaaga
     4021 aaaagaaaaa aaccgctttt ctatatcatc ttcgtttttt gtatagtcgt tgaaccattg
     4081 gtcgagaaag ctagaaagct cttcagctgt ataatgtcct gtatttatac ctaggaatgg
     4141 atagactctc agaaagcccg aaggcctttt ctctctgtag gtgcaaatcg caaacaatac
     4201 atctatgatc tgggcttcga tccatttctg tggtggacga ttatagtagg tttgtggaga
     4261 aaaggatgag ggagcaagaa aatccatgat aagaggagtt agtacgaaag aagagaattg
     4321 ttgatctgct acatgaagcg ctccattcct taatggtgga ggcttcataa agctgggtgt
     4381 tcccttaggc tccttgaatt ggccagatag atcatcctct agcaggaata atatatcagc
     4441 acaatcatta tccatcacag ctagcaggtt tctcaatttc tcaccgcctc tcttgagtag
     4501 agaggaagca agatagtcga atgatgcaat ctgtgaataa atgacttctc ttggcctatg
     4561 tcgcatagat tcgataatcg atacaaaagc gggatgagat agatttaaaa gatggcaatg
     4621 aatgtcatag aacgcagcct cttgcattat ggctccttcg ttatattgcg atttaggatt
     4681 tagctttatc tctgaacgac gagtttgcaa ctgcaatgcc taatatgatt agaattacac
     4741 tcaatccttg gctccagttg aattgctctt ttcgtatcaa gactccagcc agcactgtta
     4801 tgaccgtgac gagattggtg aacaccgcag actgcgaagc cttgagttga gagagagaat
     4861 aattgatgag gaaaaatgct cccacagagg agagtattcc tagatagact ataccgcccc
     4921 agactccagc tgttctattc cattcaccaa acaaatagcc taaattgccc ttccccttga
     4981 tattttccca gagcgcaggg atcgagaaac agatagccgc cgaccacatc atcgcaaagg
     5041 ttcgctccac tggcgaaaag ctttctgcag ctttcttgct tgtgatattg aaaaatactg
     5101 cactgagcat agatccagtc aagaatagaa cgccaatagg ctttgtttca ttattgtaac
     5161 cttgtccaaa taccgccaca aaaatcacac caacaatcga catcgcaaga ccaatcgatt
     5221 gaagagtatt gaggcgctct tttaaaagca aagcttccat tattgcaaca ccggcaggca
     5281 atgctcctag gattatgcct gcaacattag tcgcggaatt cgcaagtcca tatgtctcaa
     5341 aagtgaagta ggcaataggt tgaaataagc acataagcgc tagaagcctg atgggtttcc
     5401 ctttgaagtt cacctttatc atccctgcga cggcaagaat actcatcaat agagcggcaa
     5461 ttacgaagcg catggcaagc agctccattg ggttcagaac ttcaagagct tcctttgtca
     5521 taagaaaaga aaagccaaat attgtggaat agcctatacc tgctagaata ccgcccattt
     5581 tattcatatt tatagtatat tatagtaata ttagataaag gaaatagcca tgatgcaaac
     5641 cgatgtcgag gccttctgga ctagtttcga acaagaaatt ggagacaaaa ttatctcgaa
     5701 gactatgggt cagcatttta gctcgaagaa atcacaaggc gaatggggcc ttcttgtgtt
     5761 gaccacttta tctattcgct ttcggcctac tccaggcgag aactggttcc aatctctatt
     5821 tagaatggct acacctaaag tccctaagga accagcctcg gacatcgttt tacccttagc
     5881 cttgataaca tcgatcgagc tgcccaaaaa gcatttcttc gactttcttt ttagtcctcc
     5941 ttttacaatt tttactttga gataccgcct tggagacgaa gagcatagtg ttttattggg
     6001 agcagatccc aaatcagaat tatttacgaa gcttatgtca acaattcctg tcatgaaggg
     6061 aaaatgacta atatttatac acagctcgaa aaagcgaact tgcttttctt cgaatatggc
     6121 attacagcgc ttgattcata tttcgatgag aaacctcggc caataaaatt tcttgcggta
     6181 caatgttcgc tgatagagtt agctcgagct tttgcctctc tggaataccc tggtctaccc
     6241 tatgccgatg catctctata cgatgaagcg cgatttatct gtagcgaaga tgcgttagaa
     6301 gaagaccttg gtaatccttg gatgaatttc agaagaaatc cctccaatgg cgtattctac
     6361 gataaaggca atatatataa ttcccttaag cttcgaagca ttcccacact cttctcagag
     6421 tctccagagc tagaaaggtc attattcgat ttcaccctca tggctacctt ctctgagatt
     6481 cctcctagct tacctaaatc actttctgtt ccgacttcgc catctatcct tttacagcga
     6541 gatcttcttg taggcattct ttctgggccc tatccagcct ccggatttga agtactcaag
     6601 caaagcggat ttattgcgcg agaatggccc gaaatcgcgc ttctcgacaa tgtcgatcat
     6661 tcaaaggact tccatcccga aggtaatgga tggaaccata ctatgcagac attttcgtat
     6721 cgaaagaatc gggatcttat tctttcactt gctctgcttc tccatgatat cgggaaatgg
     6781 gaatcgaaaa gctatgaagg ccatcgcttc gacagacatg ctgaattagg agcaagacgt
     6841 gcagaaaaat ttctttctcg tttaggcttc tccgaagaaa tccaaaaaga tgtcaaattc
     6901 ctcattcgct atcatatgat gccagcggca ttgcctacgc ttcctattca aaagacagag
     6961 gagctgataa gggatccgcg ctttcctctc ttgctcgagc tctttcgttg cgatgaattc
     7021 tcgagtttca aggatgccga acgatattat gatgcatgca acacctatcg atctatttgt
     7081 cgcaatctca gaaatccata tagaaaagcc gatggatcta aagtctatgc atcaagacgc
     7141 tcagattcgc acttttcatg aagcaagtag ccatggacta taagacagag cccaccgcgg
     7201 tggcctaaaa gaaacaagtt ctccatagtg atctataagt aaacttacta ttttttgatc
     7261 cagcggcatt gatttcattt tttggaatgc acttagagga catattggat cgcgataagg
     7321 gcgctctcct acaagtgctt cgaatacatc gactacggcg atgatctttg actccaaggg
     7381 aatatccttg cctgtcaagc cctcgggata tccggaacca tcttctctct cgtggtgata
     7441 gagcacaatt gggctaagtc gctcatattc cttaaagttc ctaagaatac gcgcagaaat
     7501 cgctggatgc tgtttcatct gtacaaactc gcatgaatcg agcttgccat ttttggtaag
     7561 gatgttgttt ggtataccta ttttaccaat atcatggagc agtgcagcaa tatagagatc
     7621 cttgagtttt ttgcctctaa atccgagatt cttcccaaca aaaagagcaa gcatggcaac
     7681 attcctcgaa tggctcgcag agattctttc agtctgctca aagagatata tgatagattc
     7741 gagaccgcga gtatccagaa aggtgttagc tttcctataa taatgctgaa aaatggaaac
     7801 agcgataaac cctataagca gtactataat gatggaatcc aataagctat ggcttgtgaa
     7861 attagcagat attggaattc cataagtggt ctttctacca tgcactaaat cgatcctgaa
     7921 atccttagcc gagacagtat ctaatagtct ctgggcctgg ataatcgcca cgccaaccat
     7981 atttggaacg gtagtttcat cgtcgccgtt gcggattgga cactctattc gcaatagttc
     8041 tccatcgaga atgacatcaa aactatctgc tggtggcctg ccttctacaa tttctaccag
     8101 atctatcaag ggaaaggaat cagttgcttt ctgcacaagg atatttgcat ccgcaagttt
     8161 ctcttctata actaagtaat aggtttggtt acgtgaaaaa cagccgttgt agagggcaga
     8221 cgcttgtatc tcgatgaggc ttgaaatcgc attattgaca gattcgaaac ttcgacctgc
     8281 aatatgcctc tgaatagaat aaatcaatag tccgatcgaa agcaaactta ccagtgtaat
     8341 catgatcgga acaaacaact ttcttttcat acaacatttc ctaataataa ctaacaaaat
     8401 gatcgagaat tatttattcg actttttgtg ctccttataa tctgcaacta ttgctttagc
     8461 atctgcttct gcactatctg gaacaattac ctttgcacct tttggttcta tcgaataaaa
     8521 gggatttata tccagcatct gatcagagag tacttcagct ggaataccag cagattcgag
     8581 catagttttt acaacaattg aatcctcgag aaatgcagag atgaatacag ttttcatcta
     8641 tttgccctta cattctctat agcttcaatg ctcttgtcga atgctggccc taatctccta
     8701 gctcctttgt caattatgca ccaatcttcc tcaatgcgca tccccccaac cggcatccat
     8761 ttttcaataa tatcatagtt taggaaattt tcacagatac gctcagttcg ccatttttgc
     8821 acaagctgag gaataaaata tatccctggc tcgaccgagt ggaccatccc tggcttaagc
     8881 gtctttgcaa gcctgagcga gcgcaatcca aactgctcgc ttcgctttaa ttcttcacca
     8941 tatcctacat agtcttcgcc tagaccttcc atgtcatgga catccaggcc aatctgatga
     9001 cctaaaccgt gaggaaagaa taaagcatgt gcacccgatt caacggcctc gaccggatcc
     9061 cccttcataa ggcctaaaga tataagacct tttgtgatag tcagagaagc tttcttgtgc
     9121 acatcgagaa acctcactcc aggagcaagt gcatttgtgg ctgttctaaa ggactcacat
     9181 agaacttggt agatttcttt cgattgatgg ctaaagcttg gtcctacagg gaatgtcgtt
     9241 gtaagatccc ctgcatagcc caaaggtgtt tctacaccag catctaagag aaaggtatca
     9301 tgcgctgtgg ctatatgctc ggtgggggag ttgtggagga ctgaagcgcg acaggttgca
     9361 acagttgcga agctgagttc acagccttgc gcgctagcgc gagatagcac aaaacctgcc
     9421 gcatctttct ctgtccatcc tgcctttagt tgatgcaaaa gctcttcatg gatgttcttg
     9481 gtaatagata cggcccgttc aatttcagcg agttcttcgg gttcttttat ctctcgcaca
     9541 cttacaagag ccatgattac acgtggatcg atatatcgat agataagctc ctctaagcta
     9601 gatctagcgg tgccgaatat ctcagaaagt tgcgcaatct ttctatcttg ataaggagga
     9661 agaatgaaga caggctctct aagctttagc gtttgctcta cattattaga gagatatgaa
     9721 gtcggtaaga ctttctcaat gcctacgcta taggcttgat ctacaggaga aggcaattct
     9781 cctgtccaga tgatctcctc aagccctagc tcttcggcat aaaggcagct ttcctcactt
     9841 tcaatgtcca aagtgcaggc gagatcagga atatttaggc ctatcaagta taaccaagag
     9901 gaatcctgcc taaagggata gcagttatcg ggatagttac gaggacagtg actatttgca
     9961 agcaataaaa ctgtgcctct cgttattcct cgatcgcgga tcgcttttaa gaatgcttgc
    10021 cttctttttt tatatacttc ttgcataaac atgaatgcat tgtagatatt ctctatagag
    10081 tggtcaatca tttttgaaaa aattaggatg gatctaaatt tattggttta atccttcgtt
    10141 atagaaggta atttgcatgc tctcggtgcg gagacaagcc tcatattgag gtaatgtata
    10201 ttaaatggat tacgtgcgat attgcttcgc ttctaatctt cgcttaagaa gggttattct
    10261 ccagatgtcg caggaagagc tcgcacatct ggctaacctc tctcctggtt ttatcgcaaa
    10321 tattgagacc ggaagaaatt tcccttctag taaaggaatt ctgaagattt catcagcact
    10381 aaaggtcgag ccatggaaac tcttcctcga tcctcaaaag caagatatgt tttttacccg
    10441 cgatgaggtt tctcattggc tcgaagactc tcgcaaacgt ctttttggag atttctcaca
    10501 aggcgaatac cacacagaaa atgattcaca gatagataag gacgacatta caccgcaaga
    10561 ttgacaacaa agtgctcagt ttttagtata agcaggtgga acataatagc agatctctat
    10621 ggatagccat tctcttttta gtgcttgcaa aaggcttttc tgcttctgca caatgtcaaa
    10681 tcaagactct cgcaaacgga atgacagtat gtctcaatcc catcgaatat gaagaagata
    10741 tatctataat ccttgcctat cgcgcggggg ctaattctca aacagcttca actgcaggtc
    10801 tattcaagct tttggaatac gtactattta atggtcctgc agcgaatcct ggcattccag
    10861 agcctgctgc cgcgatagat gtgctcgaag cagagaatat aataggtgga gtagggatcg
    10921 atagatttga atttggtata atgttcaaaa aagagaacct aatcccagct cttgacactt
    10981 ttctctatct cttttcgctg gaaagaagag aattcattct tgctcaaata gatggcatag
    11041 agttggcaaa gcggtctgtc ttggctctca ttcagaatga aacctctgat aatgatcttc
    11101 taagttcgat ggcaatcaat aataagcttt tttctaaagc accttttaga accgatatat
    11161 taggagccga aaatgtttta gaaaaagcag atgctgctca gttgaataac atcgcatctt
    11221 cctggcttgt tcccaacaat gcttgcatat caatttcagg tgccttcgac tctagtcagg
    11281 ttatgaattt gatcgaagaa cgctttagct tgctgccaaa agccaagaat ccatggccag
    11341 caaatctagc ttcatttccg aagccgggcg tcacaaaacc aatatttctt gtcttgccag
    11401 atagtagcat tccagcagac cacatacaaa tagaaatgcg atacagaggt ccagatccaa
    11461 aagacactac ttcttatttt gcggcgctta tgctggagca actcgtaaac gaacctacaa
    11521 gtcgatttca gactgcaatg cgcaaagcga tgcccaaggg atctgaaccc gaaaatcttc
    11581 ggatcgacta tgtgcctgct gctaattcct cctggattgg tgttcagtct attatgccag
    11641 caggcaagaa acctgccgat accgtcttcg cattcaaaga agccgtgcgt ggcaccgagc
    11701 tctatatgat caaagcaaat ccgtcttatt tttctgctac agattatgag caagctcgtc
    11761 aagcgctgtt ggaagagata atggcaatac aaggagaccc actgcgctcc gcttgccata
    11821 tggccagctt atggagttgg ggcataccct cattcatttt tcaagaaggc gatatcatca
    11881 tgaaaaccgg gcagaaagaa gtctcccaat ttgtcgatat ttatgtgcaa aaaaatctag
    11941 aagtggtaat ggtacgtata gatccaaacc tttatgaagc caacaagaag agtttttcga
    12001 gctatggatt cgaaacggta agtgctaaga atgccttttg gtggcgctga ataaggccag
    12061 attatatcgg ttcttcttcg atagaaagga tgcttgggag cgttcgtaaa gccttggcga
    12121 cacgctttaa gtcttcatgt gaatcgactt ccagaataaa aaagccagaa agggtcccat
    12181 ccccgcgctc tcctagcttg ccttccttga gtcgtcctga gcatttgcga acggtagatt
    12241 ctatctctga gaaaatgtcg aaactttttt tggtggttac tctatagcgt gctgtcgtat
    12301 tgattgcttc gtcttcccat ttgacatcga tacgccgttc ttcgaaatct gaaatcgaag
    12361 cgatggatct gcaatcttcg cggtgtacga taattccacg accacgcgaa acatagccta
    12421 taatgcgatc gccagtaacc ggtttacagc agcctgcgat acggatcatc atgttgcgca
    12481 tacctcctac cgagaccccc gctttctcgc tgcgaagcac cagaccaggc tttaggctat
    12541 ggaattccac tacagaagga aagctctcgc tgcggcgagc ttcggtttga gaccgatcgg
    12601 cggctccatg agtacgagct tgaaccgttt cctgagtagc tttttcgctc ttgcgagccg
    12661 caacaatgtg tttgtctatt gctactgctt ggccggtctg gacaagaagc tgacgtatct
    12721 ttgagcgcgc tttgcttgtg cgagctatct tgagccaatt gagattaggc tttgcgttgg
    12781 gtgaagttat aatttctacg atctgggtat tgcgaagctc agaatctaga ggtgcgatgg
    12841 ctccattgat ctttgcgcca aggcagtgcg ccccaacatc agagtgaatg gcaaatgcaa
    12901 agtctatagg agtcgagcca gagggcaact caattatatc tccttttgga gtaaagacaa
    12961 atatggagtc acccaaaaac tcttgcttta tttcttccag atagctgtcg ccttcggcga
    13021 taaactggtt ccattgcctt agcttgttta ttatgggcag atcttctagc ttaggaatct
    13081 ctgccctggt accttttttg tatagccaat gcgatgctac tccgtattcc gccacaagat
    13141 gcatctctct cgtacgaatc tgtatctcga gcagttgccc tccataggca attactgttg
    13201 tatggaggct ttggtatcca ttcgcttttg gcattgcaat ataatctttg aatcgtccat
    13261 cgatgggttt ccagagccga tgcacgagcc caagtaggga atagcaatca ttgatgctgt
    13321 tgcagataat ccgtattccg agaaggtcat atagctcttc ggctcctttt ccgcgtctgc
    13381 gcattttctg atatatcgag tagaaatgct tggcgcgagc gctcacttcg acatctatcc
    13441 cctcttttgc agcagcggaa agtatcttat cagtcacttt ctttagaaat tcctggcgct
    13501 cattcttacg cgaatttaca atatccttga tctggtcgaa cacctcgcgg tttagcgact
    13561 tcaaggatag atcctctaat tcatctttaa gccaggaaat accgagccta tcagcaagag
    13621 gggcgtagat atctatgcac tcggtggcga tttctttctg acgatcttcc ggtaggtatt
    13681 ttagagtgcg catgctatcg agtttatctg ctagtttgac aagaatcacg cgaatatcgc
    13741 tggtcagggc aaaaagcatt tttcgcatcg tttcggcagc atgcacagtt ttattctttg
    13801 ctttgaccga agagagcctg ttgactcctg caacgatcat tgctgtttga gggccaaatt
    13861 gttcctgaat aaaaagatct ggggctgcaa gaagggaatt caatgtggtt gaaccttttt
    13921 tcttcaatgc acgctcgcct ctaggagctt gcagtatccc tcttgactga atctgggagt
    13981 cttcaaatgc agatttatct ttgctggcgg gatttccttc aattttgcta tccgaattcg
    14041 cttcatgggc aaagccagat attcgctctt cgattgtgtc atgaagcagg cctgcgagga
    14101 ttgattctag gtccataccg aggtcgagga gtatttcggc aacgcggata tcatggtaat
    14161 aggcgggctc acctgatgct cgctttcttt ctccatgagt atggcgagaa tggtctaaag
    14221 cctttaaaag aaggtttttc tcttcatcgt tgaagcgata atcgatcttc gaggaaaaat
    14281 cctgaaccag ttcatccatg acggccgcct actaccctta tgcttcctga aagatccttc
    14341 catgttctta gatctatata acccattttt ttaaagagag ccaaagactc cgatgtttgg
    14401 tagggatcca tctctaataa taatacacca ttatgggata agtattcata tccctccatg
    14461 attatcttcc gaatatgctt catgccgtcg ataccgccat ctagagagat aagaggatcc
    14521 ttccatcctt tggaaataaa tcgctctgct tcgcaatgcg gaatataagg agggtttgct
    14581 gtaataattt cgaactttcc gtctattgca gaaaagagat cggattctac aaaaatcaag
    14641 gaagtagcag tcaaaagcct ttgtgcattt ttctttgcaa tgtcgagagc tgcagatgat
    14701 atatccgagg ctgtcaactt aaaccacggc ttttcggctg cgaggcttat tgctactgcg
    14761 ccacaaccag tgcaaagatc gagaacggaa atttctctgc ctgcttcaag atcttcttca
    14821 atgtctgcta caggctcgaa tagcttcgag gattcgagtg cagcttctac cagaatctct
    14881 gtatcttgac gtggcgataa tacatcatcg ttgacaagaa attcacgacc atagaactca
    14941 cgaaaaccgc gaatatgggc tatgctttcg ccatttcttc tgcgttctgt catatcgaag
    15001 aatgactgcg gaatagaatc tgcgcactcg gggagcatcg aaaagagctt ttccttcgat
    15061 ataccgaggc tatgcgctaa aagcaacaaa gcatccaaaa aaggagtatc gctctcagaa
    15121 aagctgcaag actcattaaa aataagttct cgataggtca tgacaactag cgttcttcag
    15181 ctattagtgc ctgctctcta tcccatgcta tcagtgcatc aagcaattca tctagatcac
    15241 cttccatgat gaggtctagt ttatatagag tcaaatctat cctatggtcg gtcaagcgat
    15301 tctgaggaaa attgtaggtc ctgatacgct cggaacggtc tccggttcca acttggtttc
    15361 gtcgctccgc ggaacgctct gcttggcgtt tatcatcttc gatctcgaaa agccttgccc
    15421 tgaggattct catggccttt gccttattct ttatttggct cttctcatct tggcagtgaa
    15481 caacaatgcc gcttggaata tgtgtaatgc gcacagcgga gtcggttgta ttgacacttt
    15541 ggccaccagg accggaggac cgcattacat cgatgcgcag atcttctggc cttatctcta
    15601 tctctgtctc ttccatttca ggcaataccg caactgtcac cgtgctggtg tggattctgc
    15661 ccgaaccttc agtgaccggc acacgctgca ccctatgaac tccggattcc aggcgtaatc
    15721 tctcgtaaac tgaatcccca gaaatagaaa agattatttc cttgattccg cctagctctg
    15781 tgttgttcga actcatgatc tcgacattcc actctctgct gtcggcatag cggctataca
    15841 tcctatatag gtcagcagca aaaagagcag cctcttctcc tccagtacct gctcgtatct
    15901 ccatgatgac cgatttgtcg gccatggggt ctcgtggcgc aagaagagcc tcgagccgct
    15961 cttcggcaag agcagattga ttcctcaact gatcgagctc ttcctcagca agctctctca
    16021 attcctcgtc ctttgtttcc tgaattagtt cttctgtctc atgcatcgaa gagcaaagcc
    16081 tttgcgtctc atcgaatgca tcgagaattt ctgaaattct tgatctctcc ttcataagat
    16141 cgcgataatt cttctgatcc cgaagtacat ccggtttcga aacgagatca tctatttccg
    16201 cgagacgctc tcgcagttgc tccatgtgtt ctatgtattc tgtcacgact taatggtatc
    16261 ctgtttttgg tctgagcttc aactcctgct tgcgcgttgt tgttcatctg gatagtatct
    16321 agccatgtta gacatacgtt ttatcaaaga taacttagag gcggttcgac aaaatattcg
    16381 cgaccgttat atggatgccg atgtagacct gctgatctcc ctatacgata agaagaatgc
    16441 attgctccgc gaactcgagg aatccaggcg ttgcagaaac gagaatgccc aagccatgaa
    16501 gtcaaatcta tcgaaagaag aacgctccat tcgaatagaa gaaggcaaaa ggctcaagga
    16561 gacaatagct cgcctcgaag cagagcttca ggaagtcgag gcagcttttg ctgaagaatt
    16621 ggccaaaatc cccaacatgg ctcatcctca ggctcctcga ggaaaggaag ataaagacaa
    16681 tctcgaagtg aaaagagtcg gttctccaac gcagttcagt tttcccccca aggatcatgt
    16741 ggaactcggc cagagcctcg atatactcga cctcgatacg gcgacgaagg tttcggggac
    16801 aaaattctat tatttaaaaa acgagggaac catcctcgag cttgcactag tgcgatatgc
    16861 cttggatatg ctctcaaagc acggctttac tcttatgaca acgccagaca ttgccaagac
    16921 agagattctg gagggaatag gcttcaatcc acgaggcgct gaatctaatg tctatactct
    16981 cgaaggtgaa ggaacttgcc taataggcac agctgaaatc acgctcggtg gttatcatag
    17041 cgattctatc cttgaaaaag accagcttcc aatccgctat gcaggccttt cacattgctt
    17101 taggcgtgag gctggcgccg cagggcagtt ctcgaagggt ctataccgtg tacaccagtt
    17161 cacaaaggta gaaatgtttg tctactgcct accagaggaa tccgaggcaa tacatgaaga
    17221 gctccgctcg attgaagagg agattttttc gagcctcgat attccattca gagttgtcga
    17281 aacctgcaca ggagatcttg gagccccagc ctatcgcaag tgggatttgg aagcctggat
    17341 gccaggaaga aataatggcg aatggggaga aataacatcg acctcaaatt gtactgatta
    17401 tcaagctaga agactcaatg ttcgctatag agacaaggat ggcaaaaatc gctttgtgca
    17461 catgctaaat ggtacagcca ttgcctgttc tcgcgccatt atcgctattc tcgagaatca
    17521 tcagcaagcc gatggctctg taaggatacc taaagcctta gtaccatact gcggattcga
    17581 tctgattcag ccccatcacg tttgaggcag gcatagcgct taagacatct gaggggcatt
    17641 gagggcatcg aaaagcatat agtatatttt ggttacctaa ggcaaattag gcgaaaaaat
    17701 catcggcttg cgccacggct gctgggtgcc aggattctcc caacggattc tacgtcttat
    17761 ttccagttta cccgaaggtg actgcacaca aaccgcaaaa aaaccgctcc caatatagat
    17821 tagcttttca tctggtgcga gattctcttc cttggaaagg atttcaattg cacaatcgaa
    17881 atgtatgggt tcgccgtctt cccttgaagc tatcgcacct gcaaaatcaa agataggctt
    17941 accgcagact ctacatatct tgggttcttg tcttgggctc tctgccgatg gtttccccgc
    18001 tctaacatta ttctcttgag catggtctac catcttttgt gcggcggcat gggccgatcg
    18061 acgctttcta aaatgctgtt ccccaatttt tttttctggc tttccgtgtc taagtgaagt
    18121 gttcggacgc ttatttgatt ttggttcgga gttcatttcg atttattcct tattgtattt
    18181 gataccattt ctgcaataga agcaatcgcc ccatccaaat agtcgatatc cacaagctta
    18241 tgcttgatct cataaagcct tttccagttt gtggccttca tctcttcggt aatgtcgtag
    18301 tctgaataca ataaatcatc tggagtagaa ccagcccatg ctggaatatc tctagaaacc
    18361 tttttttgct tcatctccat cttccgaaaa tgcaccttct atgtgcctca gacaggcatt
    18421 ctcttgtatc agcataacat caaatcgaat gtgataccgt ttatattttc gattaataac
    18481 gagaaatagt ttagaggttt ctataataga agtgcgcttt ttaattccta tgacatgttc
    18541 gagatcgcgc ttagtgattc ggtttgcgga tttgacttcg acaaaagata gaatattttc
    18601 gcgaaccgct acaatgtcga tttcccctcg cctactgcga aaattgcgag caagtataat
    18661 ccagccaata ctctcgagat attggcctgc aataatttcc tcttcttttc cccttctttt
    18721 cttcttttcg ctatcggttt tcagcataag gaccatgttc acatatacgc tcttatcttt
    18781 aattcgcgca aaaatggact cctcgcttca aaactgtttg gaaagatctt gctttttaca
    18841 aaaaaagctg ccaaggctta gactctggca gctcgatatt caaccactct gaagttaaaa
    18901 actattatgc ttcctgcttc tggctcttgc gacctccaac aagagtcttg actttcgact
    18961 tcttacctac cttgctgcga atataataga gtttagcgcg gcgcactttt ccagccttta
    19021 ttatctccac tttttcgaca cgaggtgaat tgaaaggaaa tacacgctcc acgcctacac
    19081 catacgaatt ctttctcacc gtaaaggtct tccctatccc tgcgttctta atcgctatga
    19141 caagcccttc ataggcttga actcgctcag tcttgccttc tacaatttta aagcttacac
    19201 ggacggtatc tccgaccctg aaatcagaca ggtctgttcg aatctgttcc gcttgaatct
    19261 gctgtatgag gtccatgtcc tcccccttcg tctatcactt cttttaacaa tctgcctaat
    19321 tcactggtca gatcgatatt ttgcaaaagc tctggtctgt atacgagcgt ttttataagg
    19381 ctcgctttga gtcgccatcg ctctatcgct gcatggtctc cactcaccaa tacttctggg
    19441 acaaataaat tatcatattt ggcaggcctt gtatactggg gatactctag aaggcctttt
    19501 gaaaaactct cttccgaaag agagtcacct gaaataacac cgggtataag gcgatacacc
    19561 gcatcgatca caaccatcgc cgctatctct ccactcgaaa gcacataatc cccaatagaa
    19621 accacatccg taattcttgt atcgattatt cgctgatcta ttccttcata tcggccacaa
    19681 agaaatataa gattttcctc gcgggcaaaa tccagcgcca tcgcctgatt gaaaagccta
    19741 ccggacgggg taacatagat tgtcctcagt tctggagacc ccgccgctgc aagagcccta
    19801 tctaaaggct ctggcttcat cagcatgcct gggccacctc caaatacctc atcgtcacat
    19861 ttacgatggc gatcaaaagc atagtccctt atattgacaa gctcataaga tatgagcccc
    19921 ttagctacag cgcgtttcat tatggaagca gaaaaataag cttcaagaat ctctggaaat
    19981 agcgaaagca gcttgattct cattccagaa tccatgggct aataatctcg agttcgccct
    20041 tctcttcgtc gattttgccg acgaattcct ttcggaaggg gacaaggatt ctttcttcta
    20101 gatctctctg aatttccaag agatcgcttt ctgctccctc gataactgca gtgatcatcc
    20161 caacattcgc tccgtcggct attactgtca ttccaacaag atccgcgata tagtattctc
    20221 cagatttttt gggactggcc tcagacctcg gcagaaagat atcccgtccg actaactggc
    20281 gagctttttc gggcgtttca aatccctcga atactatgct taccccccag cttgccttta
    20341 ataaacgcaa aactcttatg ggcttggcat ctttcaatcc ttctctaccg cccaaaaaga
    20401 attcttgagc accttcgatg tgcgaatact ctcccgaata gctgaataat ctgagctcac
    20461 ctgccagacc ccttacggca ccgagccttg ccacagcgag atattctggt ttttgcctat
    20521 catgcataat gaaaaatcaa actcagtcaa gaatctccaa gatcacacgt tttgacttgg
    20581 atgatgcaga agcagaaaga atagtccgaa tcgcacgagc gattctgcca ttttttccta
    20641 tgaccttgcc tatgtcgctc tctgcaaccc ttagctcgag aattgtcgat ttctctccct
    20701 ctactacctt gatatcgacg gattcaggat gatcgactag tgatttaaca atatattcaa
    20761 caagatcgcg ttccatagtg ttctcgctgc tcattttagc tggatgcctt ttttattcaa
    20821 aagagaacgc acagtgtccg agggttgggc accttttgca agccactctc ttactttggc
    20881 ttcatttatg cttatctgct tgtcctctgc ttctattgga tgatagtatc caatctcatc
    20941 aatagtcttg gaatcgcgag ccgagcggga atccatgacg acaatacgat aatatggtct
    21001 ggccttggca ccaaaccttt tgagcctgat acgtacgctc acactaatcc tcctgcatac
    21061 aaaaaatgca aagtgggctt cgccttatcc gcacatccgt tccttaacaa agcccgatct
    21121 atggaacatg tgcgggaaag ttatatccta tcaatggcca aatagtcaac gcggcagctt
    21181 gactattttc aattttacat ttatggtaat aatgtcaaaa tagttggatg gagggtttta
    21241 gcaatggcaa atacaatccc tcagatgtac attgaaatcg ctcaaagaca gcccaatgtc
    21301 ccagtacagc tttctaaaga tgcgcaaggc gattttcaac ctataactta tggtgaactc
    21361 ctcaaggaaa tcagcatatt tgcgcttggg ctaggtgatt ttggcattaa aagaggagac
    21421 aatataggtt ttatttcaga taaccgaaaa gaatggcttc ttgcggacct cgctatgctc
    21481 ggaattgggg ccgtcgatgt tccgcgtggc tgcgatgcaa cagcccagga aatagcatat
    21541 attctaagct ggagcgaatg ttcgaccacc attcttgaaa acgaacgcca actaaaaaaa
    21601 atcctcgatg tcaaacaagg ccgcatttct ttcaactcaa tgtcgcttat agaagctcct
    21661 tatgccaatc tgcggtccat aatcttaatc gatccaccat catcgcaaat tgccgaagaa
    21721 gcaaggcagg cagggctcac tatttctata ttctctgaaa tcatggagaa aggcctatca
    21781 ctctataaga atgatccaga tttctatcaa aaagaggctc aaaaaggcac aagagctgat
    21841 cttgctacca taatctacac ttctggtacc acgggtgaac caaaaggcgt catgcttagc
    21901 catggtaatt ttctccacca aaccgaatac ttcccgtatt tgcttgaagt taatagtggc
    21961 gaggtgtttc tttcggtgct tccaatttgg cactcattcg agcgagctgt ccaatacatt
    22021 cttctacaag ccggatgcac aattgcttat tccaagccta taggctcgat tcttatggaa
    22081 gatatggagg tagtgcagcc ccactggttt tcaaccgttc ctcgcatctg ggaatccgta
    22141 aaggatggag tggacaagac aatccgccag agtggaggac tcaccaagtc tatgttctcg
    22201 atgttcgtag gcattggaaa ggcacatgca tatttcaaga atatgcttta cgaccagctg
    22261 ccaaaattct ctcctagatc gcgatttgtc gatatttcta tatcaataat tcccttcgcg
    22321 ctccttaccc ctttgcgcgc tttaggcgat gcgcttgtgt ttaaaaaaat caaggagaag
    22381 cttggaaagc gtttcattgc aggcgtttct ggcggaggag ccttgccgcc ttcagtcgat
    22441 cacttttttg aagctatcgg aattttaata ctcgaaggat atggcctgac tgaaactgca
    22501 ccagtaataa gcgtaaggtt gagaaatcat ccagtaacag gcacagtagg tccagtacac
    22561 cgaggaacag aggttcgcat tgtagatgag cgagggaatg tgcttcctgc agggcaaaag
    22621 ggcgttattc atgttcgcgg tcctcaagtt atgcttggat attacaagag gcccgacctc
    22681 accgaaaaag tgctctccaa ggatggctgg ctggacactg gtgatctcgg tatgttcact
    22741 atccatggtg agcttaagat aatcggccgc gtaaaagaca cgatagtatt gcgtggaggg
    22801 gaaaatatcg agcctttgcc aatagaacag agattgtgcg agagcccata tattcagcaa
    22861 gctgcagtag ttggccaaga ccaaagatat ctagcagctc tcatcgtacc aaaccaggag
    22921 gtccttaccg cttgggcaga agagaacgat attccattca acgattatcc gtcgctattg
    22981 gaacagcccg aagtaaaaga actcatcaat agcgagataa acagctatat cagcataaag
    23041 aatggattta agcctttcga gcgcatatat cactttgctc ttttgccttc tcctttcgag
    23101 gaggggcgcg aattgtcggc aaaactcgac ttaaagcgcc atgtgatcaa tgagctatat
    23161 gcggatcaga taagacaact ctttgaagaa tgattattta tagatgagga aattagttat
    23221 aagagttatt ttgcaataat tcttctgata gtagcccaaa gaacaaaaga ctcaagaaat
    23281 ctcgatatcg ccaagaatgc tctttacata gaggcgtctg gacccttctc ctgtacggaa
    23341 ggtacagcgc tgaggcccta tgccggaagc gctgactgga aagcttgctt ctattcgacc
    23401 cgaaagcgtt tctgctctga attctatgct gctatattcg agctgtatgg atatatcgcc
    23461 tgaatatgtc tggacttctc cgccaccaaa tgcaccgctc acttccagtt cgatatcgcc
    23521 cttgttggaa atgagtctaa ctcttccctt gactctgcgt gttattatct ttccctgatc
    23581 gcatactgct gctacattct tttcgacctg atctatcaag acaaaactgc tctgagtctc
    23641 cacctggact gaacctgcaa ttctcgagca cctaatagtg ccgtttatgc tccgcgcaga
    23701 acatccttca ctgttatcta ctatgatatt gccattgacg ctcgagactt ctacaaattt
    23761 ggcattcgaa atgctaatgt ctccccgatc ggtaatggca agaacatttg caggaatatc
    23821 gtgaatctct atcgatcctc tagaggaatg cacttcaata tctgatatat gagaagggat
    23881 aaaaatattg cattttccga ttctaacaaa atcattccct attgcttcga taatcgcgat
    23941 gctctcggct cgccgaaacc tgcaagacca tgtgtcgatc tctccaggaa ccccttgaag
    24001 ttccacaaaa tatcgcagcc gtgggtcttc tgatctggtt actatgatat tgccttctga
    24061 aacacggata actatcctcc ggatgccttc cacatcttga atatcttcta aaaaccggaa
    24121 atcaatatca attccttcgg tgcgcatcaa attatgcctc cgggttctca atccatgc
//
LOCUS       DAOSOB010000026        23884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_2133_length_23884_cov_60.316925, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000026 DAOSOB010000000
VERSION     DAOSOB010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 23884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23884
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2133_length_23884_cov_60.316925"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            490..828
                     /gene="tnpA"
                     /locus_tag="PLS88_04265"
     CDS             490..828
                     /gene="tnpA"
                     /locus_tag="PLS88_04265"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOU06597.1"
                     /translation="MPKWKKLAHVVYQCSYHIVWTPKYRYRILEGDIAKMVEHKIKAV
                     CEWKGIEILELNIRKDHVHMVATIPPRISISEAMGIIKGKTAIALFKGHTNFKEKPYW
                     GIISGVGDIV"
     gene            1027..3822
                     /locus_tag="PLS88_04270"
     CDS             1027..3822
                     /locus_tag="PLS88_04270"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU06598.1"
                     /translation="MLNAKISFSLRNMSFLRKLAQFAMLFLFLLGVSAQEKRAALVIG
                     NGAYTNVPRLNNPVNDVKDVVAALKVLGFEVIEGEDLDLAGMEESLQAFKKILEGKDV
                     ALFYYSGHGVQVQGENFLIPVRERIESEITVRSRAMPLSQVLNTIRDANVSTALIFLD
                     ACRDNPFPGASRSGVRGLSIVSSPKEIETLISYATEPGETASDGTGRNGIFTAAFLKN
                     LSEPGLEISELMRRVRADVIEATNGNQRPRTDIGLSKPFYFADPELLAQRAKEASDKA
                     AAELSSIEEQIAALQAQINSTKDEETRQRLLLEQQRQQAIEAAKKLEAAQLADQAQRQ
                     AEIASQTKALEEKRKKDLASAQDLQNALAQLAAEKRAELDKLAANALSESPDVLVDTV
                     ERLEKAIGEVESEYAKVLEISAASLTKTWQVKLDSLSSFQPDPWESDAMFSARIDKER
                     KELIVAQDADIARLKAEIQDQKDKQIASWRQQLQDTLKILDEKTWVVSGSSVIIDVGT
                     YDRNAMFWPFIVSSKDPSLPISGLKLKADLSKSQDIKADYLKIDTAIKTNALTAELRW
                     GIDRQKEQNRYFIVLRNARIIDLNTGSVIADQEYANRLACFEPGKWNELETSGFFQII
                     AESGDNEPELWLDGIKLPRGTSKIELQSGSYVFEYKRSGESANRIIVEIAPGEMITVD
                     TVRRLLPLVFVEGGTFTMGSMTGNEDERPIHKVTITSFWIGKYEVTQGQYRAVMGSAA
                     MKSQGLSEGLVFSDDYPVVNVSWYDAIEFCNKLSAKEQLEPVYEIASNREVVADFSKN
                     GYRLPTEAEWEYAARGGKKSNNYPYSGSSGLDWVGWDNLNSGYHAHPVGSKDANELGI
                     YDMSGNVDEWCWDYYGLYISESQRDPTGNPNGNSRVIRGGDYLSTAPYSCSISSRSKN
                     ISTTSKMTLGFRVARRQ"
     gene            complement(4025..5410)
                     /locus_tag="PLS88_04275"
     CDS             complement(4025..5410)
                     /locus_tag="PLS88_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOU06599.1"
                     /translation="MSKTRKNSGITYSLYKRPTSAKHRLFYIKFWDEDKVNFLTRSAK
                     TEDEKEAHAEAGRILGEEDLKALALQKRIERSKEQQEKRRLEAELKALQGTEADRIAG
                     LSVNEALKIFWDPERSPYLQDLKDAGRLLSNHYINENKRNIIKYLSKYGPFNSIKMKD
                     ISFAFIDEFFRHARHNGLSRYTINSILNTMRAPCTWISARGAMSQISFKGITLPEKKA
                     RERGLITPQELEKILALETAPVWFTKDTNEARIDTKPRPRLPNSKKNKGDPPLIGLRE
                     KLAIVIGALTGMRLGEIRALRWKDVDLEQGIISVSATWADEDKHLKQPKAGSSRNVVI
                     SASLEKMLIEARKTAQELGAANDESFVLFNPADPGKPISETTIKRAWNRILRAIGISE
                     EEQKKRNLVFHGLRHFYATRLVDSGLEPQEAAKLTGHRILATLGRYSNHIQKETLQKA
                     RNILDKTLYNE"
     gene            complement(5489..5562)
                     /locus_tag="PLS88_04280"
     tRNA            complement(5489..5562)
                     /locus_tag="PLS88_04280"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5526..5528),aa:Val,seq:tac)
     gene            5930..8278
                     /gene="lon"
                     /locus_tag="PLS88_04285"
     CDS             5930..8278
                     /gene="lon"
                     /locus_tag="PLS88_04285"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOU06600.1"
                     /translation="MSASELIPIDQILPGKLPIITLIGKPIFPGIFTPIMVGKEPDIQ
                     IVEQAISADGMIGLVLQKDDSDEPSSNNLFHVGTAAKIIKKINLPDGGINIFISTFKR
                     FKIKKFISKEPPIITAVSYLDDENYDNDEVKALTRALISEMKQLSENNPLFSEEMRLN
                     MINIDNPGKIADFITSILNIEKKDQQDILETLDVRERMEKVLIHINREKELFRIQKKV
                     QAEINEKIEKSQREYFLREELKAIKQELGMAPDARSSDYQRFRDKIDSFQFEGEIKEI
                     VEQELEKFNLMEPSSSEYMVTRNWLDLVCSLPWNTSITADFDIKHAKKVLEEDHYGLQ
                     DVKDRIIEYLAVRKLKKDTRGTILCLVGPPGVGKTSIGRSVARALGKQFFRFSVGGMR
                     DEAEIKGHRRTYVGALPGKIIQGLKIAKSRDPVFMIDEIDKMGISYQGDPSSALLEVL
                     DPEQNFSFRDHYLDLPFDISNIFFIVTANTLDTIPRPLLDRMEVIQLPGYVDVEKIEI
                     ARHFLIPRSLERNGLAKNQVKYTRDALLVIIDGYAREAGVRNLEKSLDKIHRKIAKSI
                     VIENRQEEKFKIDKHTVEKYLGKPIFRDEELKRASRPGMAIGLAWTSLGGDTLLIEAV
                     ANPGKEGFKLTGQMGPVMQESAGIAYTYVRNIVAQKYGIQAEFFESRQIHLHIPEGAT
                     PKDGPSAGITMATALLSLVLNKRIKDRLAMTGELSLTGQVLPIGGLREKTVAAKRNKI
                     KEIIIPAANEKDLDEIPEHVKKGLVFHSVSRMEEVIEIVFGS"
     gene            8350..10488
                     /locus_tag="PLS88_04290"
     CDS             8350..10488
                     /locus_tag="PLS88_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU06601.1"
                     /translation="MTEVEDGNIKGMHLNPVAGQFIRPCGKGFRAHQDHISPKRLRTP
                     LLRTGSRGSGQFREISWDEALSLIAQKLSEIRSESGPGSVLCIASSGSTGALHNSQTL
                     ARRFFNSIGGCVNVEGNYSCNAATYALTRLFGTSKGNSGFDAETIRHSNFIVLWGSNP
                     FEARLGAELPAYLTEAKNRGTRIISIDPRQSLSARILNAEWIGIRPGTDAVLGYALLF
                     EILQDARFNQEFISKYAIGFEFLADYVLGKTDRIAKDPQWASSICGIDAKMIVKLASF
                     FWNEKPAMIIPGYSIQRVAYGEEAFRLTVAIQLATRNSGMLGGSTGSINNCLPGVKVE
                     TLEELPSSLTKQGSVRGSLTREAQLWKDRDISLGAEAGLFNKRVPVLRWADAVLNPSS
                     YGLRPIRVLYSAGGNFLNQGANVTKNIRAFESVDFAVCHELFLTPTANYCDVVLPVSD
                     AFEKEDIGIPWAGNYVLYKPEICRALPNTHSDYWIFSQLSERIGVEPIFTEGRTESQW
                     IDHFLAESEIDDIDAFKRHGIFIKKGQLRAGLDDFFANPVDHPIGTKSGKIEFASPLW
                     NSSMASHWQGSIKECFRQESCRRFWLLTPKVSRFIHSQRIEQEDRSECAQIHIHPEDM
                     ASLGAQKGEILKIWNSNGALLASAIPDVWLQPGIVWIEEGIWTMPIDGVDLCGNPNML
                     TSDEGTLESISNIMHGIPIQIERIAEANGQ"
     gene            complement(10520..11614)
                     /locus_tag="PLS88_04295"
     CDS             complement(10520..11614)
                     /locus_tag="PLS88_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HOU06602.1"
                     /translation="MSKLFLVGDIGGTNTNLALARQDESGIELVEKVQFSTKNEKSLL
                     DPIKRFLGSRRVRESYDRIDFCCISGAGPVQPDNSIQLTNAPWGIKASELSDFLRVPV
                     RLINDFTALSYAVTTLDYKDRSAVTQLPHPDGSIYEPREGIMLVIGAGTGLGVGFVDR
                     RGDSVMAFPSEGGHSELPCFDDLSDSFGTWLRKRYDYAPGAELAISGQGIANIFEFIC
                     SEDFNVILVGDDYEIPQKALIEGLSETARNILNLPRSEWPAYISKNARSDSFCSLTME
                     LFVRLYALKAANLVSIFLPAGGVWLAGGISSKNEDWLIEKARFMRWFEKNYAPHIRDV
                     LCRTPVLIVKNYDISLMGAAIAALQFATHV"
     gene            complement(11636..12550)
                     /locus_tag="PLS88_04300"
     CDS             complement(11636..12550)
                     /locus_tag="PLS88_04300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU06603.1"
                     /translation="MENDFYQNLSMLNENRFSSLSTEQIEKLASTLCIQGLTMDLDNR
                     AMASLFFPSSGHVLAMLIDDEDSEEYQNLDGISFPARLSYNRIASLHPKMNALRTKYE
                     SFFDVFYSMSKSNALTFPTKWFESNFVAWNGNVTLPGLSSLPRLNELDYSHTFALDIF
                     LQNVEYLSWNIQRGPIIHSLSDGIVVSAESGWLGFPRKSTALSYIKGGISPKSGNGVI
                     VYSPEEHRYYLYFHLYDLFVEKGDILLRGQPIGHGGNTGINARKKGGGDHLHLEIFDS
                     KKGRFLRNTQIITLLKNSSKNRLAVSPR"
     gene            complement(12776..14524)
                     /locus_tag="PLS88_04305"
     CDS             complement(12776..14524)
                     /locus_tag="PLS88_04305"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOU06604.1"
                     /translation="MNHEEIILRAQEYIANETDPSFSDEIKELLKKSDWKELEDRFYR
                     DLEFGTGGLRGVIGGGFNRMNTLVVTRATQGLCDYIKEQFPKKQLSACIAYDSRRKSK
                     EFSLATALVFAANGIKAYIFPSLKPTPVLSYAIRKLGADTGVVVTASHNPPQYNGYKA
                     YWNDGSQVVPPHDSGIIEKVLKVKSVKQISKTEASSKGLLMEIGQEIDDDYVAMVKSH
                     LLRSYLFSETGKSVNIVYSPLHGTGAMLFERIMKELGLNVLTVPEQREPDGEFPTVSY
                     PNPEESAALAMAIELGKKTHADVVMATDPDADRLGIAVPDKTGEFVLVTGNQLGSLHL
                     DYIALTLKELHRMPPRAAAIRTIVTTELQKVIAEKYGIESFECLTGFKWIADLMRRFE
                     TENYDFIYATEESYGHLIEKEVRDKDGISAAALTAEMTLYWRSQGKSLLDHLEELYKE
                     HGYYEEKGLSFYFEGEQGMRIMNSIMEDYRKQQPDQFGGLSVICIRDVKASTEWDRNG
                     RIRKIDLPKSDVIQWRLEDGTLLTVRPSGTEPKIKYYILCHDHNSDLSLSKEITRKKI
                     SIIEQAITAMVDSHRI"
     assembly_gap    14528..14627
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14833..15504)
                     /locus_tag="PLS88_04310"
     CDS             complement(14833..15504)
                     /locus_tag="PLS88_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOU06605.1"
                     /translation="MPKIKPKFIIFDLDGTLADTIVDIGSSVNFVLSQFDLPIHKISD
                     YKHMVGDGFRVLVERVLPQDKSNDDRLFDEIFKLSMRRYEEHVLDETKPFPGMIETLE
                     YLYRKGLKLAVLSNKPDRLSKKIIESLFASVDFVAIWGDNKDRARKPDPSAALELCRI
                     AKANPKQSLFVGDSAVDMQTAKAAGIAAVGALYGYRSREELELAGADYLISSPQELLS
                     IVESG"
     gene            complement(15543..15893)
                     /locus_tag="PLS88_04315"
     CDS             complement(15543..15893)
                     /locus_tag="PLS88_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06606.1"
                     /translation="MEFSEKLKKFMENSAEASREFLEKAADQAQVWGEMGKLKIEILQ
                     LRNKAQSLTAKLGAEVYNLLIEKNEPMIGSSTPEIEPIIRDLKDLDRLIDEKESLYRS
                     KGGKESDLNLQSRE"
     gene            16103..17017
                     /locus_tag="PLS88_04320"
     CDS             16103..17017
                     /locus_tag="PLS88_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOU06607.1"
                     /translation="MLWIYIIFAVLSAWWVVFIIIAAARKQKPVFVFLLVLFLVWAIP
                     EIVLMIISPPVATILFIVPFIILIQMMRVVQEYQRGVLFRFGRLQKVIEPGFNLILPF
                     GIDSLRKVDMRTFTIDVPKQEVITRDNVPVIVDAVVYFNVFDPMLAVVKIADYTKSTS
                     LLAQTILRSVLGQHELDEMLSQRAQLGQILQRLLDEATDPWGIKVSAVEIKAVELAET
                     MKRAMARQAEAERERRAKIIAAEGELQASEKLAQAADVLAQSPASLQLRYLQTLTEIA
                     VEKNSTIVFPLPMDLLKVISKAAEKSSE"
     gene            17014..17481
                     /gene="nrdR"
                     /locus_tag="PLS88_04325"
     CDS             17014..17481
                     /gene="nrdR"
                     /locus_tag="PLS88_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOU06608.1"
                     /translation="MKCPYCGSLEDKVIDSRTLANGEAIRRRRECISCGLRFTSYERI
                     EEKPLLVIKRDGRREPFERQKIERGLVRALEKRPISQMSIENLVNEIEDEAAEHAKAS
                     NEISSEELGRMVLQKLYSLDKVAYIRFASVYRKYDTLDEFIDEIERLENGQTC"
     gene            complement(17506..19602)
                     /gene="fusA"
                     /locus_tag="PLS88_04330"
     CDS             complement(17506..19602)
                     /gene="fusA"
                     /locus_tag="PLS88_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU06609.1"
                     /translation="MSYTTEQIRNIAIIGHGSTGKTTLLEHILFQGGMISRPETVDSG
                     KTVSDYSEDEIARKISVRSSLTHVNTDDCKINFIDAPGAGDFVGEAILAIRSTETALL
                     VIDGKSGVQIETVKLWRILERQKRPRMIFVTRLDEDRANFSNTLADIKEKFRVSPVPV
                     TIPMGEGTSFRGIIDVLNNKAYMRPSSHDQKEVASEVPAEYSESVESARALLSEAAAE
                     GNDELMEKYLIEGELTQEETLLGLKEALAEGKIIPAFAGSGFANSGTAALIDFIMQSA
                     PSPFARAPEKAKDQDGNDLEMSIDPSKPASAFVFKTQIDQFSGRLSYIKAITGTILSD
                     MDMVVSRDLHKERIGKLFTMQGKKLEEISSLPAGDIGVLAKISSLKTNDTISQFDKPI
                     SYAPLQLPSPVHMLAISAVNKKEEDKLNELLNRAAEEDLTFRVSYNPETKETVIAGMG
                     EQQINMILDKIKSQSKIAAETRIPRVAYRETITKKATAEYTHKKQTGGHGQYARVVFE
                     IEPLERGKDYEFENRIFGGAVSKGFMPGIEKGIHQAMEAGVLAGYPVVDTKTAIIDGK
                     EHPVDSSEMAFKLAARGAFREAMKQASPVLLEPIMNLSVFVEDKYLGDVMSDLSGRRG
                     KISGQNPIGGGIVQIDAQVPQAELLRYAIDLRSMTSGTGSFEVEFSHYAPISGKIAED
                     VIKAAQAFKTQEAEEE"
     gene            complement(19726..20520)
                     /locus_tag="PLS88_04335"
     CDS             complement(19726..20520)
                     /locus_tag="PLS88_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU06610.1"
                     /translation="MDSILDALQEGRLFELPENDKDHALQFLAHIIEAFPQIPTGTDI
                     VGNVMAREKSMNTALGKRWACPHARVEFEEDLMCVVGWSPTGIDYGAPDGVPVSIIVM
                     YLVPSNQRNHYLREISTLAKVLKSSPEIDKLSTIKELNDVRNYLLDLISVSKETAGPD
                     ARARMIRLQAKAAMATQPVPELSNLIIEPVSIIAGVGIKPFALTQNLELMNFIETTPG
                     LTERIETDGKYQNGLWRIIRRSATAYQGGRTIFDCIALMPASTGQR"
     gene            complement(20523..23177)
                     /gene="mgtA"
                     /locus_tag="PLS88_04340"
     CDS             complement(20523..23177)
                     /gene="mgtA"
                     /locus_tag="PLS88_04340"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF011702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HOU06611.1"
                     /translation="MTKFPLILPSIRKKTETKNGSISSANENKLISICLTPLQSVFET
                     LESGPQGLSEKEADKRLSEFGKNEIAQLKKLNFWEDMLDRFKNPLVIQLLVIAAISSL
                     IGEMQSAAVVGAMILLSVGLSYILDNRSNREVESLGKRVQSRTYVLRNGIETEIRMSE
                     VVPGDIVLLQTGAIVPADVRIIWAKDFFVSESALTGESMPVEKSAALPTGTIRSAIEL
                     PNACFMGTSVTSGSARAIVVSTGTNTLFGTISKKLIERREESSFDKGIRSFTWLMIRL
                     MLIMVSVVFLIVGVTKGNWLEALLFALSVAVGLTPEMLPMIVTVNLAKGALAMAKKKV
                     IVKKLPSIQNLGAINILCTDKTGTLTQDRVVLEHHVDIIGNKSDEVLNYAYLNSYFQT
                     GLKNLLDRAVLEHVDLNVDECQLVDELPFDFQRRRMSVIVEYEGDNVLICKGAVEEIY
                     SCCSYYQIDEEIYPLIDMIRADLFEDVEKLNTEGFRVLGIAYREFPRQKTTFTVEDES
                     QLILLGYIAFIDPPKESATKAIELLSKTGVEVKVLTGDNGLVTKKVCKDVGIPFTSSI
                     TGPELAALDKDEFSDKVRKCEVFVKLTPNQKEEIVKELRRQGNIVGYLGDGINDATAL
                     KAADVGISVDSAVDVAKEAADIVLLEKSLLVLEEGIMEGRRIFSNIIKYIRMGSSSNF
                     GNMFSMLGASFLLPFLPMKPLQILANNLLYDISQTGIPMDNVDPELVAKPVRWDIDNI
                     KRFMMCIGPISSIFDYATFALMWFFFNAKNYLNPALSLAERNAAASLFQTGWFVESLL
                     TQTLIVHIIRTKRIPFFQSRASAPMILATLVVMGIGIWLPYSPIASILGLVPLPGIYW
                     IWIVAFLLIYSIITHKVKCWFFKHFEGA"
     gene            23545..23617
                     /locus_tag="PLS88_04345"
     tRNA            23545..23617
                     /locus_tag="PLS88_04345"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23578..23580,aa:Pro,seq:tgg)
     gene            23693..23766
                     /locus_tag="PLS88_04350"
     tRNA            23693..23766
                     /locus_tag="PLS88_04350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23727..23729,aa:Arg,seq:tct)
     gene            complement(23812..23883)
                     /locus_tag="PLS88_04355"
     tRNA            complement(23812..23883)
                     /locus_tag="PLS88_04355"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23848..23850),aa:Gly,seq:tcc)
BASE COUNT         6629 a         5041 c         5362 g         6752 t
ORIGIN      
        1 aactgaaagt gcgagcttgt ggatttcgca atagagctcg attcaagatg gcaatcctct
       61 ttcattgtgg gaaactctct ctgcttccag aggtattatt atgacccaca ctaaagccgg
      121 aagcgccaga aaattaaata aatccaagac tgtattaata catatccacg aaatatcctt
      181 ggtggcttta cccctttgca atgctttaaa gaggcttttg gactaaccaa tcagactatc
      241 aaactgctgg aggcatgctc attaaatatc cactacaaaa acataagaat aaattatgtg
      301 gacaatttac tttacagaag atacatttgg acttcatatt ttaagtagtg tcagagaaaa
      361 gactgttatg gcacatcttg caatactctc acttttgaac tttattagga ccttggaaaa
      421 ccaccgcctt tggcggtggt gatgtcctga ctggctatgc cgtaagatag ccaggagtta
      481 caatgaggta tgccgaagtg gaagaagctt gcgcatgttg tctatcaatg cagctatcat
      541 atcgtatgga cgccgaaata ccgatatcga attctcgaag gcgatatagc gaaaatggta
      601 gagcataaga tcaaggcagt atgcgaatgg aaagggatag agatactgga gctcaacatc
      661 aggaaggatc atgtgcatat ggtagcgacg ataccgccga ggatatcgat atcagaagcg
      721 atggggatta taaaggggaa gacagcgata gcgctcttca aagggcatac gaatttcaag
      781 gagaagccat attggggaat catttctgga gtaggggata ttgtgtgacg accatcggga
      841 tggacgaaga taagattcgt cgttatgtaa aataccagga agaaagggag aaacaagagg
      901 agaataactc gaaggattat gggctctttg agagcccaga ttcttaaagc caccaccttt
      961 ggtggtggtt gtttacttag tttataatta tcaaagagat attcaacata ttttaggaga
     1021 ctaccaatgt tgaatgcaaa gatatcattt tccttaagaa acatgtcttt tttgcgcaaa
     1081 ttggcacaat tcgcaatgct attcctattt ttgcttggtg tatctgcgca ggagaagcga
     1141 gcggcgctcg tgataggcaa cggagcctat accaatgtgc cgcgtctcaa taatccagtc
     1201 aacgatgtca aggatgttgt cgcagcattg aaggttctag ggtttgaagt gatcgaaggc
     1261 gaggatcttg atcttgcagg aatggaagaa tcgcttcaag cgttcaagaa gattcttgag
     1321 ggcaaggatg ttgctctttt ctattattct gggcacgggg ttcaagttca aggagaaaac
     1381 ttccttattc ctgtgcgtga gcggatcgag agcgaaatca cagttaggag ccgagctatg
     1441 ccattaagcc aggtgctgaa taccattcga gatgcgaacg tgtcgaccgc gctcattttc
     1501 ctcgatgcat gcagagataa tcctttccca ggagcgagca gaagtggagt gcgcggattg
     1561 agcatcgtca gttctcctaa agaaatcgag actcttatct cgtatgcgac agagccaggg
     1621 gaaactgcat ccgatggcac agggagaaat ggaatcttca ccgctgcttt cttgaaaaac
     1681 ctctcagaac ccggattaga gatttcagag cttatgcgaa gggtcagggc tgatgtcatt
     1741 gaagccacga atggaaacca gaggcctcgt acggatattg ggctttcgaa accattctat
     1801 tttgccgatc ctgaattgct tgcgcagcga gcaaaagaag caagcgacaa ggcagcggca
     1861 gagctttctt caatcgaaga gcaaatagcc gcattgcaag cacagattaa tagcactaag
     1921 gatgaagaga ctaggcagag actgcttctt gagcagcaaa gacagcaggc catcgaagct
     1981 gcgaagaagc tcgaagcggc tcaacttgca gaccaagcac agaggcaagc cgaaattgct
     2041 tcacaaacaa aggccttaga ggagaagcgg aagaaggatc tggcttctgc acaagatttg
     2101 cagaatgcgc ttgcgcaact cgctgcagaa aagagagcag aacttgacaa gctggcagcc
     2161 aatgctctca gtgaaagtcc agatgttctt gtagataccg tagagcgcct tgaaaaggct
     2221 attggagaag tcgagagcga atatgccaaa gtgcttgaaa tctcagctgc cagtctgaca
     2281 aagacatggc aggtaaagct ggattcactc tcttcttttc agcccgatcc ttgggaatcg
     2341 gatgctatgt tttctgctcg tatcgataag gagagaaagg agctcatagt cgcacaagat
     2401 gcggacattg caaggctaaa ggctgagatt caggatcaaa aagacaaaca gattgcatct
     2461 tggagacagc aactccagga taccttgaaa attctcgatg aaaagacctg ggttgtttca
     2521 ggtagttcgg tgattattga tgttggtaca tatgatcgca atgcaatgtt ctggcctttt
     2581 atagtgagtt cgaaggatcc gtcgcttcct atttcaggac ttaagctcaa agcagatctt
     2641 tcaaagtcgc aggatatcaa agctgattac cttaagatcg ataccgcaat aaagacaaat
     2701 gctctgaccg ctgaactccg atggggaatt gatcggcaaa aagagcaaaa tcgatatttt
     2761 attgtcctta ggaatgcaag aataatcgat cttaatacag gatctgtaat agctgatcaa
     2821 gaatatgcaa atcggttagc atgttttgaa cccggaaaat ggaatgagtt agagacaagc
     2881 gggttttttc agattatcgc agagagcggg gataatgaac cagaactctg gcttgatgga
     2941 ataaaacttc ccagaggtac ttcgaagatt gagcttcaaa gcggcagcta tgtctttgag
     3001 tataagagat cgggagaatc tgcgaatagg ataatagttg aaattgctcc tggtgaaatg
     3061 attacagtgg ataccgtgag acgacttcta ccattggttt ttgtagaggg aggcacattt
     3121 accatgggtt ctatgacagg aaatgaagat gaaaggccta tacataaagt gacaattact
     3181 tctttttgga ttgggaaata tgaagtcacg caaggacaat acagagcagt gatgggaagt
     3241 gccgctatga aatctcaggg attatctgag ggattagtat tctctgatga ttatccagta
     3301 gtcaatgtga gctggtatga tgctattgaa ttctgcaata aactttcagc caaggaacag
     3361 cttgaacctg tctatgaaat agcatcaaat agagaggtag tggcggattt ttcaaagaat
     3421 ggctatcgac tgcccacaga ggcggagtgg gagtatgcag cgagaggtgg aaaaaaatcc
     3481 aataactacc cttattcagg ctcaagtggt ctcgattggg taggttggga taatttaaat
     3541 tcggggtatc atgcacatcc cgttggctca aaagatgcaa atgagcttgg tatctacgat
     3601 atgtcgggta atgtagacga atggtgttgg gattattatg gattgtatat ttccgagtct
     3661 cagagagacc ccacaggaaa tcctaatgga aattcgcgtg tgattcgggg aggtgactat
     3721 ctttccacgg caccctattc ttgcagcatt tcatctagaa gtaaaaatat ttctactaca
     3781 tcgaaaatga ctctcggttt ccgtgtcgcg cgaagacaat aatttcggta ttttatccga
     3841 agtccttaag aatatatcat gcctgacagg agagcgcatt atacaaatat ttatcttata
     3901 aagattaaaa caaagaccgg cacttcttca gtggtagtaa tacctgccta tcctgcagta
     3961 tggcttatag attctgaatc tcagtattaa caaggattaa gagcataagg cattaacaat
     4021 ctaattattc attataaagt gttttgtcta aaatatttct tgccttctgc aatgtttctt
     4081 tctgtatatg gttcgaatac cggccaagag tagcaagtat acggtgccca gttaattttg
     4141 ctgcctcttg tggctctaaa cccgaatcta caagtcgcgt tgcataaaag tgccttaatc
     4201 catgaaatac tagatttctt ttcttttgct cttcttcact tattccaatt gctcgcagta
     4261 ttcgattcca tgccctttta atggtggttt cagatatagg ttttcctgga tcagcggggt
     4321 tgaataacac aaagctctca tcgtttgcag ctccgagctc ttgtgctgtc tttcttgctt
     4381 caattagcat cttttctaga cttgcagaaa taactacatt tctgcttgat ccagcttttg
     4441 gttgcttaag gtgtttatct tcatcagccc atgtcgcact gactgaaata attccttgtt
     4501 caaggtcaac atccttccag cgcaatgctc taatttcacc tagcctcatg cctgtaagag
     4561 cgcctataac gatcgcaagc ttttcacgta aacctattaa aggcggatct cctttattct
     4621 ttttgctatt tggtaagcgg gggcgcggtt tagtatcaat ccgagcttca tttgtatcct
     4681 ttgtaaacca cactggcgcg gtttctaaag caaggatttt ttctagctcc tgaggtgtta
     4741 tgaggccccg ctctcttgcc tttttttcag gcaaagttat tcccttaaag gatatttgag
     4801 acattgcgcc tcgtgctgat atccaagtac aaggagcacg cattgtatta agaatagagt
     4861 ttattgtata tcgtgacagc ccattatgcc ttgcgtgcct aaagaattcg tctataaatg
     4921 cgaaactgat atccttcatt ttgatcgaat tgaatggacc atactttgaa aggtatttaa
     4981 ttatatttct tttattttca ttaatgtaat ggtttgatag cagtctacca gcatctttca
     5041 aatcctgaag atatggcgaa cgctctggat cccaaaatat tttcaaggcc tcatttaccg
     5101 aaagaccagc aattctatct gcttctgtac cctgtaatgc ctttagctcc gcttcaagcc
     5161 tgcgcttttc ctgttgctct tttgagcgct caatgcgctt ttgcaatgct aatgctttga
     5221 ggtcttcttc tccaagaatt ctccctgctt ctgcatgcgc ttctttttcg tcttcagtct
     5281 ttgcagatct tgttagaaag tttactttat cttcatccca aaactttata tagaatagtc
     5341 tatgttttgc agaagttggt cgcttgtaaa gactgtaagt aattccagaa ttctttctag
     5401 ttttcgacat gatattgcct cccaagaatt ggtgacactt tcttagtgac agtccgattt
     5461 tggcaatgca atatcatgta attccttatg ggcgctgacg gatttgaacc gccgacctac
     5521 tgggtgtaaa ccagccgctc tgaccaactg agccaagcgc ccaaactatc catttgtgca
     5581 gcggctgaac actagcataa ggcagccaaa tgagtcaagc aaaatagtct ttttggaaga
     5641 gcgaatcatt ggatttgggc ataacatata cgatatcaaa gaaagactcc atttcgataa
     5701 atggctggaa ttcaaaataa tgttcccaat attggatctg tacaagccaa tattgggaat
     5761 tcgtataaag cgcgcgatac actctccatt cttaagagaa gatgaggtag cagactcgaa
     5821 gaatccttgc cgaatagcct taattttccg tacatttata atgtcggatt ctattccgca
     5881 atgcaaacat tactattatt ttaattataa ttctattagg tggtataata tgtcggcaag
     5941 cgagctcata ccaatagatc aaattttgcc tggtaagctg ccaataataa cacttatcgg
     6001 aaaaccaata ttcccgggaa tctttacgcc aataatggtt ggcaaggagc cggacattca
     6061 aattgttgaa caagcgattt cggctgatgg aatgattggt cttgtgcttc aaaaagacga
     6121 ttctgatgag ccatcctcga ataatctatt tcatgttggt acagcggcaa agatcataaa
     6181 aaaaatcaat cttccagacg gtggcatcaa tatattcatc tccactttca agcgtttcaa
     6241 gataaagaaa ttcatctcca aggagccacc aataattacg gcagtcagct atctggacga
     6301 cgaaaactat gacaacgatg aggtaaaggc tctgactcga gcccttataa gcgagatgaa
     6361 gcaactcagt gaaaacaacc cgcttttctc tgaagagatg cgcctcaaca tgatcaatat
     6421 tgacaaccct ggaaagattg ctgacttcat cacgagtatt ctcaatatcg agaaaaaaga
     6481 tcagcaagat attctagaga ctttagatgt gcgcgagcga atggaaaagg tccttattca
     6541 tatcaataga gaaaaagagc tctttaggat tcagaaaaaa gttcaagccg agatcaacga
     6601 aaaaatcgaa aaaagccaac gagaatactt tctacgcgaa gagctcaagg ctatcaaaca
     6661 agaacttgga atggctccag atgcgcgaag ctctgactat cagcgcttta gggataagat
     6721 cgactcattt cagttcgaag gggagatcaa ggaaatcgtc gaacaggaac tcgaaaaatt
     6781 caacctcatg gagccttctt ccagcgaata catggtcact agaaattggc tggatctcgt
     6841 gtgttctctt ccatggaata catcgataac agccgatttc gatatcaagc atgcaaaaaa
     6901 agtgctcgaa gaggaccact atggtctaca ggatgtcaag gatcgaatca tagaatacct
     6961 ggctgtacgc aaactcaaaa aagacacgcg tgggaccatc ctctgtcttg ttggtcctcc
     7021 aggtgttggc aaaacgagca ttggtaggtc ggtcgcgcgt gctttaggca aacaattctt
     7081 tagattctct gtaggaggaa tgcgcgacga ggccgagatc aaaggacacc gcaggaccta
     7141 tgttggggcc ttgccaggca agatcatcca aggtctcaaa attgccaaaa gccgcgaccc
     7201 tgtatttatg atcgatgaga tagacaaaat gggtatctcc tatcagggag atccatcgag
     7261 tgctctgcta gaagttctcg atccagaaca aaatttcagc ttcagggatc attatctcga
     7321 cctgcctttc gatatctcga atatcttttt tatcgtcacg gcgaatactc tggatactat
     7381 tcccaggccg ctgctcgaca gaatggaagt gattcagctt cctggttatg tggatgttga
     7441 aaagatcgaa attgcacggc actttcttat tccgcgaagt ctcgaaagaa atggccttgc
     7501 aaaaaatcaa gtgaaataca caagagatgc tttgctcgta atcatagatg gatatgcccg
     7561 cgaggctggc gtacgcaatc tagagaagtc tctagacaaa atacatcgta aaatcgcaaa
     7621 gagtatagtt atcgagaata gacaagaaga aaaattcaaa atagataagc atacagtaga
     7681 aaaataccta gggaagccaa tattccgcga cgaagaactc aagcgtgcca gtaggccggg
     7741 tatggccatt ggattggctt ggaccagtct tggaggggat acattgctaa tagaagccgt
     7801 agccaaccca ggcaaggagg ggttcaagct aactggtcag atggggcctg taatgcagga
     7861 atcggcagga atagcctaca cttatgtaag gaatattgtt gcccagaagt atggaatcca
     7921 agctgaattc ttcgaatcta gacaaataca ccttcatatc ccagagggag ctacaccaaa
     7981 ggatggacct tctgcgggca tcacaatggc cactgctctt ctttcgctag tgctaaataa
     8041 gagaataaaa gatagattgg cgatgactgg agagctgagc cttacgggcc aagttcttcc
     8101 tatcggagga ttacgagaaa aaaccgttgc agcaaagcgc aataaaataa aggagatcat
     8161 aataccagcc gcaaacgaga aggacctaga tgaaattcca gagcatgtca agaaaggact
     8221 tgtatttcat tcagtatccc gaatggaaga agttatcgaa atagtgtttg gctcatgata
     8281 gaatcatgaa gcatggcact tttgcctttt ttctgtggaa aagattgcgg aggagatgct
     8341 tgtccccttt tgaccgaagt tgaagatggc aatataaagg gcatgcatct caacccagtt
     8401 gcaggtcagt ttattaggcc ctgtggcaag ggctttcggg cccaccaaga tcatatctcg
     8461 ccaaaaagat tgaggactcc tcttctgcga acaggttcaa gaggttcagg ccaatttcga
     8521 gaaataagct gggatgaggc tttatcactt attgcgcaaa aacttagtga aatccgatcc
     8581 gaaagcggac caggctcagt tctctgcata gcaagtagcg gttcgactgg agccctgcac
     8641 aatagtcaga ctctagctcg tcgctttttc aattctattg gtggatgtgt aaatgttgag
     8701 ggaaattatt catgtaatgc tgcaacatat gcgcttacaa gactttttgg aacatcgaag
     8761 ggaaatagcg gatttgatgc ggaaacaatt cgccattcga atttcatagt tctatggggc
     8821 tcaaatccat tcgaagcaag gctcggtgca gaactgcctg cttatttaac tgaagcaaag
     8881 aataggggaa cgagaattat ctctatcgat ccgcgacaga gcctttctgc aagaattcta
     8941 aatgcagaat ggattggtat acgtccaggc acagatgctg ttctaggcta tgctctctta
     9001 ttcgaaatac tacaggatgc acgattcaat caagaattca tctccaaata cgcgattggc
     9061 ttcgaatttc ttgcagatta tgtgcttggc aaaaccgata gaattgcgaa agaccctcaa
     9121 tgggcaagct ctatctgtgg tatcgatgct aaaatgatcg tgaaattggc ctcctttttc
     9181 tggaatgaaa agccagcaat gataattccg ggttattcta ttcagagggt ggcctatggc
     9241 gaagaagctt ttcgtctaac cgtagccata caacttgcaa ctcgcaatag cggcatgctt
     9301 ggtggatcca caggatcgat caacaattgc cttcctgggg tcaaagttga gaccttagaa
     9361 gaattgcctt ctagtttgac taagcaaggc tcggtcaggg ggagcttgac tagggaagcc
     9421 cagctatgga aggacagaga tatatctcta ggggcagaag cagggttgtt caataagagg
     9481 gtgcctgtac ttcgctgggc tgacgctgtg ctcaatccat cttcctatgg gcttagacct
     9541 attagggttc tatattcggc tggaggaaat tttcttaatc aaggcgcaaa tgtgacaaaa
     9601 aatattaggg ccttcgagag cgttgatttt gccgtgtgtc atgaactgtt tcttacccct
     9661 acagccaatt actgcgatgt ggttctgcct gtttcggatg cgttcgaaaa ggaagacata
     9721 ggtattccat gggcaggcaa ctatgtactc tataagcctg aaatatgcag agcattgcct
     9781 aatacgcatt cggactattg gattttttca cagttatccg agcgtattgg tgtagaacct
     9841 atctttacag aagggagaac tgagtctcaa tggatagatc attttcttgc tgaatcggaa
     9901 attgacgata tagatgcttt taagcgccat gggatcttca ttaaaaaagg gcaattgcgc
     9961 gctgggctag acgatttctt tgccaatccc gtggatcatc caattggaac taaaagtgga
    10021 aaaatagaat ttgctagtcc tctatggaac tcaagcatgg catcgcattg gcaaggctcg
    10081 ataaaggaat gttttagaca agaatcttgc agaagatttt ggctgctgac tccaaaagtc
    10141 tcaaggttta tccattctca acgcatagaa caagaagatc gatccgaatg cgcccaaata
    10201 catatccatc ctgaagatat ggcatctctc ggtgctcaaa aaggcgaaat cctcaagatt
    10261 tggaattcaa acggagcgct tctcgcttca gcaataccag acgtatggct tcagcctggc
    10321 attgtatgga tcgaagaagg catctggaca atgcctatag atggggtaga tctctgcgga
    10381 aaccccaata tgctcacttc ggatgaaggg accctcgaga gcatctctaa catcatgcat
    10441 gggataccaa tacagataga gcgtattgct gaggcaaacg gtcaatgatt cagatccaat
    10501 tctctaatcg agagtttact tatacatgcg tagcgaattg cagagcggct atggcggctc
    10561 ccatcaggga gatatcataa ttctttacga ttagaactgg tgttctacag agtacatcgc
    10621 gaatatgcgg agcatagttc ttttcgaacc atctcatgaa ccttgccttt tcgatgagcc
    10681 aatcttcatt tttagaggat ataccgcccg caagccagac tccgcccgca ggtaagaaaa
    10741 tagacacaag attggcggcc ttgagcgcat atagcctgac aaatagttcc atagtcaagc
    10801 tgcaaaacga atcggaccta gcattttttg atatataggc aggccattca gatcgaggaa
    10861 ggttcaaaat atttctagca gtttcggaaa ggccttctat cagcgctttt tggggaattt
    10921 cgtagtcatc gcctacaagg attacgttaa aatcttccga gcaaatgaat tcgaaaatat
    10981 tcgcaatgcc ctggccagaa atagctaatt cagcacctgg cgcataatca tatctctttc
    11041 gaagccaagt accaaaggaa tcactcaagt catcgaaaca cggcaattca gagtgaccac
    11101 cttcggaagg aaaggccatt acactgtcac ctcttctatc cacaaagcct acaccaagac
    11161 ctgtcccagc cccaatgaca agcataatgc cctcgcgcgg ctcatagata gatccatctg
    11221 gatgtggaag ctgcgtaacc gcacttcgat ctttatagtc aagagtagtg accgcgtagg
    11281 acagagccgt gaaatcattt atcaatctga ctggcactct aaggaaatca gaaagctccg
    11341 aggcttttat accccaagga gcattagtaa gctgaatgct attatcaggc tgaacaggtc
    11401 cggctcctga aatacaacaa aagtctattc tatcgtaaga ttcgcgcacg cgccttgatc
    11461 cgagaaaacg cttgatggga tccaggaggg atttttcatt cttagtagaa aactgcactt
    11521 tttctactaa ttcgatgccc gattcgtctt gcctggctaa ggctagattg gtgtttgtcc
    11581 ctccgatatc gccaacgagg aataatttgc tcattcctat actatagcga agaaattatc
    11641 tcggtgaaac agctagccta ttctttgagg agtttttcaa aagtgtgatg atctgggtat
    11701 ttcttaaaaa acgccccttc ttggaatcga agatttcgag atgcagatgg tctccccctc
    11761 cttttttgcg tgcatttatg cctgtatttc ctccgtgacc gattggctga cctctcaaga
    11821 gaatatcccc tttttccaca aagagatcat agagatgaaa atatagataa tatcgatgct
    11881 cttcaggcga gtatacaatt acgccattgc ctgattttgg agaaattccg cccttgatat
    11941 atgatagagc cgtgctcttt ctagggaatc ctaaccatcc gctttcggct gagactacta
    12001 tgccatcgct caatgaatga ataatagggc ctcgctgaat attccaagaa agatattcta
    12061 cgttttggag gaaaatatcc agcgcgaatg tatgagaata gtcgagctca ttgagtcgag
    12121 gcaatgagga aagaccggga agcgttacat tgccattcca tgctacaaaa ttgctttcga
    12181 accattttgt aggaaaagtt aatgcattgc tttttgacat actgtaaaag acatcgaaga
    12241 atgattcata tttggtgcga agggcattca tctttggatg caaagatgcg attcgattat
    12301 atgagagtct tgcggggaag gatattccat cgagattttg atattcctct gaatcttcat
    12361 catcgattag cattgcgagg acatgcccag aagatggaaa gaacaaagaa gccattgctc
    12421 tattgtctaa atccatagtt aagccttgaa tacatagagt ggatgctaac ttttcaattt
    12481 gttccgtcga tagcgatgaa aatctattct catttagcat ggagagattt tgataaaaat
    12541 cgttctccat taatgaccgc tcattttgat cgggaaaggc tactttttca gaaagcaata
    12601 taggcctcgt gtcggaataa ggattaagag aaagcatcac gcaacgaaag gccaagaagc
    12661 aaaggatcga tagaattgaa atggtcacaa gtttgattat tcgaaaagcc acagacttgc
    12721 ctttcaagtt aattattcgc gatcggcatt gcttgcggat cagagctctt tttggtcata
    12781 tgcgatgaga atcgaccatt gcagtaattg cctgctctat tatagatatt ttctttcgag
    12841 tgatttcttt gcttaagctg agatcgctgt tatgatcgtg gcaaaggatg tagtatttta
    12901 tcttgggttc tgttccggat ggccttaccg taagcagcgt gccatcttcg aggcgccatt
    12961 ggataacatc gcttttaggc agatcgattt tgcgaattct gccatttcta tcccactcgg
    13021 tactagcttt tacatctcga atgcatatta cgctaaggcc gccaaattga tcaggctgct
    13081 gttttctata gtcttccatg atgctattca ttattctcat gccctgttca ccttcaaaat
    13141 agaagcttaa gcctttttcc tcgtaatagc cgtgctcctt gtatagctct tcgaggtggt
    13201 cgaggagact tttgccctgt gaccgccaat agagggtcat ttcggcagtc aaggcagctg
    13261 cggaaatgcc atctttgtct ctaacttctt tttcgatgag atgaccatat gattcttctg
    13321 ttgcgtatat gaaatcgtaa ttctcggtct cgaagcgccg cataagatct gcaatccatt
    13381 tgaatccagt taggcattcg aagctttcta tgccatattt ctcggctatg accttctgta
    13441 gttcagtggt gacgatggta cggatagccg ctgctctagg tggcatcctg tggagctcct
    13501 tgagagtaag tgcaatgtaa tcgagatgta gggatccgag ctgattgcct gttaccaata
    13561 caaattctcc tgtcttgtct ggcacggcaa taccaagcct atcggcatct gggtctgtag
    13621 ccattactac atcggcatga gtcttcttgc ctagctcgat tgccatagca agtgcagctg
    13681 attcttcagg attggggtag gatacggtcg ggaattcgcc atctggttcg cgttgttctg
    13741 gcacagtaag gacattcaaa ccgagctctt tcattatgcg ctcgaatagc attgcccctg
    13801 tgccatggag aggagagtac acgatattga ccgatttacc tgtttcggag aaaagatagg
    13861 aacgtaagag atgggatttc accatggcga cataatcatc atcgatctct tggccaatct
    13921 ccatcaagag tccttttgat gaggcctctg ttttggagat ctgctttaca gactttactt
    13981 ttaggacttt ttcgataatg ccgctatcat ggggaggcac gacctgagaa ccatcgttcc
    14041 aataagcctt gtagccgtta tactgaggag gattatggct agcagttacg actacgccag
    14101 tgtcagctcc aagctttcgg atggcgtatg ataggacagg agttggcttg agcgatggga
    14161 agatatacgc cttgatacca ttggctgcga agaccaaagc cgttgccaag gaaaactcct
    14221 tggattttct gcgagaatca taggcaatgc aggcagaaag ctgcttttta ggaaattgtt
    14281 ctttaatgta gtcgcatagt ccctgcgttg ctcgcgtaac caccaatgtg ttcatcctgt
    14341 tgaaaccacc accgattacg ccgcgcagtc ctcctgtacc gaattccaaa tctctataga
    14401 agcggtcttc gagttccttc caatcgcttt tcttaagaag ttcttttatc tcatcggaaa
    14461 aagatggatc ggtttcatta gcaatgtatt cctgggctct taaaattatt tcttcgtgat
    14521 tcatgatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt cattagcaat
    14641 gtattcctgg gctcttaaaa ttatttcttc gtgattcatg atggctcctc gatagtatca
    14701 agtcgggcta tgcataaagt atgcataaag agcaattcca atccttttta ttgtattttc
    14761 aattatagct tttatcattt gataaaaaaa gtcagccttt ccaaaaaagg atatcgattt
    14821 ttggcttccc aatcatccag actcgacaat cgagagtagt tcttgcggtg atgatataag
    14881 ataatctgct ccggcaagct cgagctcttc tctagatcga taaccgtata gagcacccac
    14941 tgccgctatt cctgctgctt tggccgtctg catatcgacg gccgaatcgc ctacgaataa
    15001 gctttgtttg ggatttgctt ttgcaatgcg acagagttcg agtgcagcgc ttggatcggg
    15061 cttacgggct cggtctttat tatctcccca aatagctacg aaatcgacag aggcaaaaag
    15121 agattcgatg atcttttttg agagccgatc tggcttgttc gaaaggacag cgagttttaa
    15181 tccttttcta tataggtatt caagcgtctc gatcatgccg gggaaaggct tcgtttcatc
    15241 taaaacatgc tcttcatatc tccgcataga gagcttgaat atttcgtcaa atagtctatc
    15301 gtcattggat ttgtcctgtg gaaggactct ttcgacgaga acccgaaaac catctcctac
    15361 catatgctta taatctgata tcttgtggat tgggaggtcg aactgggata acacgaaatt
    15421 caccgatgat ccaatatcga cgatggtgtc ggcaagtgtt ccatcgagat cgaatattat
    15481 aaatttaggc tttatttttg gcaattcgag aactccgact cttagagatt gtagatttgc
    15541 gcctattctc tggattgtag attgagatcg ctttctttgc cgcccttgga tctatataag
    15601 ctttccttct cgtcgatgag gcgatccagg tctttgaggt ctcttattat aggctcgatc
    15661 tctggcgtcg aagagcctat catgggttca tttttctcta ttagaagatt atagacttct
    15721 gcgcctagct ttgcagtgag agattgcgcc ttatttctca attgtagaat ttcgattttt
    15781 aatttaccca tttcacccca aacttgagcc tgatcggctg ctttttccaa gaattctctc
    15841 gaggcttcag cactgttttc catgaatttc ttcagctttt ccgaaaactc catttcaatc
    15901 tcctttatat atgagcataa taaataattt ctcatcgata caataatccc agatagacaa
    15961 attagcaata taatcgagta tgcgaatagc aaaatagtgg gtatgtcgat cgcccagtag
    16021 cctaatttcg ctaggacatt gtttagcttt tgcggagcag ggttaaatac attatattta
    16081 agtaaaatag ggggtatatg tggtgttgtg gatttatata atctttgcgg ttctttccgc
    16141 atggtgggtg gtttttatca ttattgctgc agcgcgaaag caaaaacctg tctttgtatt
    16201 cctccttgtg ctattcctgg tatgggcaat cccagagatc gtattgatga taatttctcc
    16261 tccggtagct actattctgt ttatcgtacc tttcataatt ctaattcaga tgatgcgcgt
    16321 cgtgcaggaa tatcagagag gggtcttgtt tcgatttggt cgcttgcaga aagtaataga
    16381 gccgggcttc aacctcatat tgccattcgg tatcgattcg cttcgtaagg ttgatatgcg
    16441 tactttcacc atagatgttc caaagcagga agtaataaca cgagataatg tgcctgtaat
    16501 tgtggatgct gtagtatact tcaatgtatt cgatcccatg ctcgccgtgg taaaaatagc
    16561 ggattataca aagagtacta gtctccttgc tcagacaatc ctgcgatcgg tgcttggtca
    16621 gcatgagctc gatgaaatgc tttctcagag ggctcaactt ggccaaatac ttcaaaggct
    16681 tttggatgaa gcgacggatc cttggggtat taaagtctca gctgtcgaga taaaggcagt
    16741 ggaactcgcg gagaccatga agcgcgccat ggccaggcaa gccgaggctg agcgagagag
    16801 aagggcaaag ataattgccg cagaagggga acttcaggct tctgagaagc tcgcccaagc
    16861 tgccgatgta ttggcacaat ctccagcaag tttgcagctc cgataccttc agacgctcac
    16921 ggaaattgca gtggaaaaga attcaacaat agtatttcct ctaccgatgg acttattgaa
    16981 ggttatttct aaagcagcag aaaagagttc ggaatgaagt gcccctattg cggaagtctt
    17041 gaagacaagg ttatcgactc tagaacttta gccaacggag aagccataag aaggcgcaga
    17101 gaatgcattt cctgtgggct tcgttttacc agttatgaac gcattgaaga aaaacctctc
    17161 cttgtcataa aaagagacgg tagacgggag ccattcgaaa gacaaaagat cgagcgaggc
    17221 cttgtccgcg ctctagagaa aagaccaatt tcccagatgt ctatcgagaa tcttgtaaat
    17281 gagatagaag acgaggctgc agaacatgcc aaagcctcta atgagatctc gagcgaagaa
    17341 ttgggccgaa tggttttgca aaaactctac tcgctagaca aagttgcata tatccgtttt
    17401 gcatcagtgt atagaaaata tgacaccctc gatgaattca ttgacgaaat agaacgccta
    17461 gaaaatggac agacttgcta gcagatagaa gagcattgct attcattact cttcttccgc
    17521 ttcctgagtc ttgaaggctt gcgcagcctt tatgacatct tctgcgatct ttccagagat
    17581 aggcgcataa tgcgaaaatt ctacttcgaa agaacctgtt ccagaggtca tggagcgtag
    17641 atcgattgca tagcgcaaaa gctctgcttg tggaacctgc gcatctatct gaacaatacc
    17701 accgcctatg gggttttggc cggatatttt tccacggcgc cccgatagat cggacattac
    17761 atcgccaagg tatttatctt ctacaaagac agagagattc atgattggtt caagcaaaac
    17821 tgggcttgct tgcttcatgg cttcacggaa agcccctcga gctgcaagct tgaatgccat
    17881 ttcagaggaa tcgacaggat gctcctttcc atcgatgatt gctgttttag tatctactac
    17941 gggatatcca gctagcacgc cggcttccat cgcttgatgg atgccctttt ctatgcctgg
    18001 catgaagcct ttggatactg caccaccaaa aatcctattt tcgaattcat agtcctttcc
    18061 tcgttccaac ggttcgattt caaatacaac tcgagcgtat tgaccatgcc ctcctgtttg
    18121 tttcttatga gtgtattctg cagttgcctt cttggttatt gtttctctat aagctacgcg
    18181 aggaattctg gtttctgccg cgatctttga ttgggatttt attttatcaa ggatcatgtt
    18241 gatttgctgt tcgcccatac cagctattac tgtctctttt gtctcaggat tgtagctcac
    18301 acggaaagtg agatcttctt ctgcagccct atttagcaac tcgttgagtt tatcttcttc
    18361 ttttttgtta actgctgaaa tcgccaacat atggacagga gagggtagct gcagcggtgc
    18421 ataggagatt ggtttatcga attgactgat cgtatcattt gtctttaacg aggaaatctt
    18481 agcaagaaca ccgatatcgc ccgctgggag agaagatatt tcctcgagtt ttttcccttg
    18541 catagtaaag agctttccaa tgcgctcttt gtgcagatcg cggctcacta ccatgtccat
    18601 atcggaaaga atagttcccg ttatagcttt gatataagag agtcgaccag aaaattggtc
    18661 aatctgggtt ttaaatacaa aagccgatgc aggcttcgaa ggatcgatag acatttctag
    18721 atcgttgcca tcttgatcct tagccttttc tggtgcacga gcaaatgggg atggtgcgct
    18781 ctgcataata aaatcaatga gagccgcggt tccgctgttt gcaaacccag atccggcaaa
    18841 ggctggaata atcttgcctt cggcaagggc ttccttaaga ccaagcagag tttcttcttg
    18901 agtaagttcg ccttcgataa ggtacttttc catgagttca tcgtttcctt ctgctgccgc
    18961 ttcagaaaga agtgcacggg cggattccac cgattccgaa tactctgccg gaacttcgct
    19021 ggcaacttcc ttttgatcat gagagctagg cctcatatat gccttgttat tgagtacatc
    19081 gattattccc ctaaaagagg tcccttctcc cataggaatt gtcacgggaa caggagagac
    19141 ccggaatttc tctttgatgt cggcaagggt attcgagaag ttggctctat cttcatcgag
    19201 tctggttaca aatatcattc ttggtctttt ttggcgctct aatattctcc agagttttac
    19261 tgtctcaatt tgcacaccag atttgccatc tatcacgagc agagccgtct ctgtagaacg
    19321 aatggctaaa attgcttctc caacaaaatc gccagcgcca ggtgcatcta tgaagttgat
    19381 cttgcagtca tcggtattga cgtgcgtaag actcgagcga actgatatct tgcgcgcaat
    19441 ctcgtcttcg ctataatcgc tgacggtttt gcccgaatct accgtttccg gtctagatat
    19501 cattccgccc tgaaaaagaa tgtgctcaag cagagtcgtt tttcctgtgc taccatgtcc
    19561 aatgatagcg atgttacgaa tctgttcagt ggtataggac atactaactc ctcacagggt
    19621 gggatggttt ataatctcct cttttgttta aggaagacga gctattataa ttcaaaagcc
    19681 gcaggtgtca acagcagctt ggcaaatata tgatatatgt aggattcacc tttgtccggt
    19741 gcttgcaggc atgagtgcaa tacagtcgaa tatagttcgt cctccctgat aagcagtagc
    19801 acttcgccgg attatgcgcc aaagcccatt ctgatatttg ccatcagttt caatcctttc
    19861 ggtcaagcca ggagttgtct caataaaatt catgagttcg aggttttgtg ttagagcgaa
    19921 tggcttaatg cctactcctg caattatcga gaccggttcg attataagat tggacagctc
    19981 tggtacaggt tgagttgcca tagcggcttt tgcttggaga cgaatcattc ttgctctagc
    20041 gtcgggtcca gcagtctcct tgcttactga tattaaatcc agtagataat tgcgaacatc
    20101 attcaattct tttattgtgg acaatttatc tatttctggt gaggatttga gtaccttggc
    20161 caaagttgat atttctcgaa gatagtggtt tcgctgatta gaaggaacca ggtacatcac
    20221 aattatagag acaggtactc catcgggagc tccataatcg atacctgttg ggctccatcc
    20281 cactacgcac atcaagtctt cttcgaattc cacacgcgca tgagggcaag cccacctttt
    20341 gccaagagcg gtgttcatcg atttttccct tgccatcaca ttgcccacaa tatcggttcc
    20401 agtgggaatt tgcggaaagg cttcgattat atgagcgaga aactggagcg catgatcctt
    20461 atcattttct ggaagctcaa aaagcctgcc ttcctggagg gcgtcaagta tagagtccat
    20521 ggttatgctc cttcaaaatg tttaaaaaac cagcatttca ctttatgtgt gataatcgaa
    20581 tagatcagaa gaaatgccac tatccaaatc caatagatgc cagggagagg gacaaggccg
    20641 agaatgcttg cgatgggaga gtagggcaac cagattccaa ttcccataac caccagtgtt
    20701 gcaagaatca taggagccga agccctgctt tgaaagaaag ggatgcgctt agtccgaatt
    20761 atgtggacaa tgagagtctg tgtcagtagt gactcgacaa accagcctgt ttgaaaaagg
    20821 ctagccgcgg cattccgttc tgccagtgaa agggcagggt tcaggtaatt ttttgcattg
    20881 aagaaaaacc acataagcgc aaaagtcgca taatcgaaga ttgagctaat tggtccaata
    20941 cacatcataa atcgctttat attgtcgata tcccatctga ctggttttgc gaccaattct
    21001 ggatccacat tatccatagg aatgccagtc tgggaaatgt catacagtag attattggca
    21061 agtatttgaa gaggcttcat ggggaggaag ggcagaagga agctcgcccc cagcatgctg
    21121 aacatattgc caaaattcga gctagatccc attcttatgt atttgattat gttagagaag
    21181 atacgcctgc cttccataat cccttcttct agcactagaa ggctcttttc cagcaatacg
    21241 atatctgctg cttccttggc aacatctacc gccgaatcga ccgatattcc cacatcggct
    21301 gctttgagag cagtagcatc gtttattccg tctcccaaat atccaactat gtttccctgt
    21361 cgtcgcaatt ctttgactat ctcttctttt tggtttggag ttagtttaac aaaaacctcg
    21421 cacttcctta ctttatcaga gaattcatct ttatcgagag ctgccagctc agggccggta
    21481 atcgatgaag tgaaggggat cccgacatcc ttgcatactt tcttagtaac aagaccgttg
    21541 tcgcctgtta gaacctttac ttcgacacca gtcttggaaa ggagttcgat ggctttggtt
    21601 gctgattctt ttggaggatc gataaaggca atataaccca acaatataag ctggctttca
    21661 tcctcgacag taaatgtagt cttttgacgt ggaaactcgc ggtaggctat gcccagaacc
    21721 ctaaaccctt cagtattcaa tttctctaca tcctcgaata ggtctgcccg gatcatatct
    21781 atcagaggat atatttcctc atcgatctgg taataagaac aacaactgta tatctcttcg
    21841 actgccccct tgcagattag gacgttatcc ccttcgtatt ctacgattac cgacattcgg
    21901 cggcgctgaa aatcgaatgg aagttcatcc acaagttgac attcgtcgac attaagatcg
    21961 acatgttcaa gtacagctct atctaggagg tttttcaggc ctgtctgaaa gtagctattg
    22021 agataggcgt agttaaggac ctcatcgctc ttgtttccaa tgatatccac atgatgttcg
    22081 agtactacac gatcctgtgt tagagtacct gtcttgtcag tacataggat attgatggcg
    22141 ccaagattct ggatagatgg caatttcttt actatgactt tcttctttgc catcgccaga
    22201 gctcccttag caaggttcac tgtaacgatc atggggagca tttcaggagt aagccctact
    22261 gctacagata aagcaaagag cagcgcttct agccaatttc ctttggttac tccaacgatc
    22321 aagaatacta cgctgaccat aatgagcatg aggcgaatca ttagccacgt gaaggaacgt
    22381 atgcctttat cgaaactgct ttcctcgcga cgctctatga gctttttcga aatagtaccg
    22441 aaaagcgtat tggtgcctgt ggaaacgact atggctcggg ctgatccgct agtaacgcta
    22501 gtgcccataa agcaggcatt aggtaattcg atcgccgatc ttatagtacc tgttggcaga
    22561 gcagcggatt tttctacagg catcgattct cctgtcaaag ccgattcgct tacaaagaag
    22621 tcttttgccc aaatgattct cacatcagct ggtactattg ctccagtttg gagcagcaca
    22681 atatccccag gcacgacttc agacattctg atttcagttt cgatgccatt tctgagtaca
    22741 taagtgcggg actggacacg cttgcctaga gactcgactt ctctattgga acgattgtcc
    22801 agtatgtaag agagccctac actcaatagg atcattgcac ccacaacggc tgcggattgc
    22861 atttctccaa ttaaagaaga gatcgccgca atgacgagaa gttggatgac aagagggttc
    22921 ttgaaacggt ctagcatgtc ctcccagaaa ttgagttttt tcagttgtgc aatttcattt
    22981 ttgccaaact ccgaaagcct tttgtcggct tccttttcgg aaaggccctg aggacccgat
    23041 tcgagcgtct caaatacgct ctgaagcgga gtcaagcaga tagaaatcag cttgttctca
    23101 tttgcgctag agatacttcc attctttgtt tcagtctttt tccttatgga gggtaatata
    23161 agtggaaatt tagtcatagt tcacctcaca tttctgtcaa tctctcctga cagaaggagg
    23221 tgaatggcat aggacaaaat gtagctctat gctttttcta taccagctgc caggtgagat
    23281 ggtcgcggga atggatcttc gggctcggta aagatgcagt gactatgact gccatccgag
    23341 tcattcatga cacatcctcc tttttgctgg gatttaatag atttagaatt cgatttacta
    23401 tgcatcatta tatggctata gtcaagccct atatgagaat ttgcagccct tgatctttgg
    23461 tgggctctta atatagcgtc catttggaga atatggatat cttgacatct agcctgtttt
    23521 tcaattagtt tacaacgctg aaaccgggac gtagcctagg ggctaaggcg tctgctttgg
    23581 gagcagaaga tcggaggttc aaatcctctc gtcccgaata ctgcttttgg atgcggcaaa
    23641 aaagcagcct ctttaaggcc tgtgcatttt ttgcttttat tgcgcactca atgggatcat
    23701 agctcaactg gatagagcaa ctgccttcta agcagtaggt cgcgcgttcg agtcgcgctg
    23761 gtcccataaa atttatttag cttatatcaa ttataatcta taagttaggt aagcgaccga
    23821 tgggagtcga acccacgtct ccagcttgga aggctggggt aatagccgtt atacgacggt
    23881 cgca
//
LOCUS       DAOSOB010000027        23655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_2167_length_23655_cov_65.874831, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000027 DAOSOB010000000
VERSION     DAOSOB010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 23655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23655
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2167_length_23655_cov_65.874831"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..2093
                     /locus_tag="PLS88_04360"
     CDS             294..2093
                     /locus_tag="PLS88_04360"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOU06612.1"
                     /translation="MITIDELRRKYIEFFKARGHAEISGKSLIPENDPTVLFTTAGMH
                     PLVPYLLGEPHPAGKRLTDYQKCIRTGDIDMVGDTSHLTFFEMLGNWSLGDYFKNEAI
                     HWSYEFLTSPDYLGIDPVKLSVTVFAGDESVPRDDESAYLWKKVGIPEERIFFLPRED
                     NWWGPAGETGPCGPDTEMFVDTGAPACGPDCKPGCHCGKYFEIWNDVFMQYNKKADGS
                     YELLARPCVDTGMGIERTVAMLQGKKSVYEIEAFTPILKVLESITGKTYGVSSSDAEI
                     DRSFRIIADHIRAAAFILGDPKAVLPSNIGAGYVLRRLIRRAVRHGKKLGIEGLFLSR
                     PAQAVIDIYKEPYPELLENAGRIFEELEREEKKFLETLQKGEHEFEKILPNLLKNPDK
                     TMPGRLAFKLYDTYGFPIELTEELAAEHELKVDKQGFEESFKKHQEISRAGSEQVFKG
                     GLADNSEIVTRYHSATHLLHKALRLVLGNHVAQKGSNITAERLRFDFSHPAPMTPEQI
                     AAVERIVNEQIARDLPVTMEMMPPEEAKAAGAIALFGEKYEPIVKVYTMGDFSKEVCG
                     GPHVSHTGEIGKFKIIKEQSSSAGVRRIYAVLE"
     gene            complement(2102..2584)
                     /locus_tag="PLS88_04365"
     CDS             complement(2102..2584)
                     /locus_tag="PLS88_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06613.1"
                     /translation="MEIELKTLHLCQSLDYVHIEEPQIVEAKTLSDFLSNKSELLLEF
                     EENCLSTMTDDGPKVFEDLKPSAVYRLAENKYIETMAPNDINLGRTSLFSLDIGKYIF
                     CQQDMYYDEEVWRLKSLLEEAIREVWWQRVKCEGSWFLRLVPEDDRVAVQALKKMRPE
                     "
     gene            2713..4689
                     /gene="dxs"
                     /locus_tag="PLS88_04370"
     CDS             2713..4689
                     /gene="dxs"
                     /locus_tag="PLS88_04370"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOU06614.1"
                     /translation="MIDYFEMSLLERISGPADLQGLSMPDLRRLSSEIRQSIIETVQR
                     NGGHLASNLGVVELTIALHRVFQSPYDAIVWDVGHQCYSHKILTGRAARFVTLRRKGG
                     LSGFPKRKESKHDVFDTGHSSTAISSALGLLQARLSTGHAGRVIAVVGDGALTAGLSM
                     EGLSNAGQLGLPLIIVLNDNRMSISHSVGSMSRYLSKLSASVRYQSLRSRVDAAVLRI
                     PRIGEALFSLINRTKRAIKAIFFKENLFVDYGFEYVGPIDGHNLVSLIDTFEHVRKLN
                     RPVVVHVVTRKGKGLEKAEEDPESFHGLGPACPDIPGPGSISHGESFTSCFANALIRC
                     AEKEPRMVAITAAMSSGTGVSRLAERYPHRVYDVGIAEQHAVAFAAGLAQGGLRPVVA
                     VYSTFMQRALDQVFQEVALAKQPVLFALDRAGAVGEDGETHQGIYDIVLFKAMPNLLF
                     FAPADGEELSAFMDLAITLDLPCMMRYPKAFSHSSTLPIAPLEIGRGVFMRDHDDARI
                     LILAVGPLSYIAAEVSDELETKGIVADVYNVRFISPIDEEHLVDICSRYDGVLTIEDG
                     VLNGGLGESVAGILMRKGMQIKISSLGFGSIPLAQATREELLSSAGLDSKGIQKALYD
                     LLNSLDLSKQQLGTGIPANLSGRLSTPSEIQHAH"
     gene            4679..5563
                     /gene="folP"
                     /locus_tag="PLS88_04375"
     CDS             4679..5563
                     /gene="folP"
                     /locus_tag="PLS88_04375"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOU06615.1"
                     /translation="MRISAKRMQAKNHSSKLRLPNGRSLEIGSAPEHLPCIMGIVNIT
                     KDSFFEGSRRLEPKAAIETALDMIRQGASIIDFGAESTRPGSKEIDPKEELSRILPVL
                     EGFRAVSDAVISVDTRHPKVAEAALAAGADIINDIEALQRPGMAEVVARGRAAAILMH
                     MQGTPQNMQQNPSYENCLEEVRSFLEGAADYAIEAGISPDAIILDPGIGFGKLKEHNI
                     AVLQGIERIASLGFPVLIGLSRKRLIGELTGKDVADRLAGSLGAALAAWLNGAQILRV
                     HDVAETLDALRAFHEVIQ"
     gene            5560..6363
                     /gene="cdaA"
                     /locus_tag="PLS88_04380"
     CDS             5560..6363
                     /gene="cdaA"
                     /locus_tag="PLS88_04380"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOU06616.1"
                     /translation="MNGPTFIQFFTVYVRPVLDVGILAFLIYKTYQLLVMTDAVYLIR
                     GLLILLAIYGISFFLKLSTVTWIMNILAPGLVIGLAIILQPELRRIFIQIGRQSIFGR
                     KSMARSSWIEAAVSASVYLAEKRRGAIIVMARKVGLASYIEKGIPLDAIISTSLLISV
                     FEHDNPLHDGAAIISQGRLAAAGCFLPLSDQQEIRKTFGSRHRAALGIAEESDAVVLV
                     VSEETGTLSLAYDSKLYYDLSSEEVLERLHILLSEQNLRREGNEVANEP"
     gene            6353..7312
                     /locus_tag="PLS88_04385"
     CDS             6353..7312
                     /locus_tag="PLS88_04385"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOU06617.1"
                     /translation="MSLDSKISKLLENWPAKVISLVMAIFIMFLYNLTRLEQRVIIVP
                     LNISEGHSYVPATEYPKTVRVIIRGDREQIYRVRESDIVASLDLARYSSEGVFRVPVK
                     LERRGDALNIDPLELKAEPSEIPISFERLAAKRVAITPTFKGFLEQGYVLISYEILPS
                     EISIEGPTSLVSSIKDISTDIIELSGKTSDFSLSVPLVKPSDLIRLVDANTVKFSAKI
                     QIQERRVTLKNVPIYISNLDPSLTISDQLPSGKITLVPQPGQDETLASDASLQADFKG
                     IVKPGLYSIPLTVNTPEGFEVEAYEPLALNVRLHSAPVLPTFH"
     gene            7331..7708
                     /locus_tag="PLS88_04390"
     CDS             7331..7708
                     /locus_tag="PLS88_04390"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOU06618.1"
                     /translation="MISGIGIDIVHVDRIRRWMDEPGILQRFFNPAEIETASFRKKGM
                     ALSLAARFAAKEAFGKALGSGLANFALKEVAVVNDSLGRPIMRLEGNARREFERHGGG
                     KIHISMTHEGDNAVAIVILEKEN"
     gene            7712..8512
                     /gene="truA"
                     /locus_tag="PLS88_04395"
                     /pseudo
     CDS             7712..8512
                     /gene="truA"
                     /locus_tag="PLS88_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
     gene            8660..9601
                     /locus_tag="PLS88_04400"
     CDS             8660..9601
                     /locus_tag="PLS88_04400"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU06619.1"
                     /translation="MTRSQLKARNAHLFCNGVEVKFSHKLKSKDRLRLEWTEAPSGQI
                     EPEAIPLEILYQDADVYVIDKPQGMVTHPAAGNWHGTLANAVLWLDHEALQNIQVQDS
                     FSISSPARAGIVHRLDKDTSGVIIVARTATAQEFLSSQFREHQARKEYFAIVRGIPPE
                     PKGRIDTWIARNQRDRKKFTVSEPGRGKHALTLYRVCRAWEANCYIDDRLVDVASKGA
                     KPSIYSLLALYPRTGRTHQLRVHCANIGCPILGDPIYGKKDEIFPEATLMLHAHRLKI
                     RLPSRSEPSVFSSPFPTRFRDIISYLDKHGHNIKVTD"
     gene            complement(9817..10113)
                     /locus_tag="PLS88_04405"
     CDS             complement(9817..10113)
                     /locus_tag="PLS88_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOU06620.1"
                     /translation="MSKKIYVGNMNYNTSERQLQDLFAQYGDVSSVNIIVDRFTGKAK
                     GFGFVEMDNPDAASAAIAALNGQEFMGRQLRVNEAQEKPRYEREDTGGYRGRRY"
     gene            10586..12118
                     /gene="rny"
                     /locus_tag="PLS88_04410"
     CDS             10586..12118
                     /gene="rny"
                     /locus_tag="PLS88_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOU06621.1"
                     /translation="MAPLVGYIVLPLAGLILGWMIRWLYARFRLSSAEREAERVLQDA
                     IKEAEAKKKEILVEAKDQIIRERNQQERELRDRRMELQKYERRVLLKEENLDAKLASI
                     ERIEADLKNRENKIAEKEEYLAGQEERYAAELERVSGFTAEEAKKLIIQNMEQEARRD
                     AQTLINKIDQEAQLSAEKKAKDILITTIQRIATESTAESTISSVSLPSDEMKGRIIGR
                     EGRNIRTLETLTGVDIIIDDTPEAVVISCFDPIQREIAKISLERLIADGRIHPARIEE
                     MIQKVTREINQKLYDEGEKVVFDLGLHNMAPELIRAVGRLYYRTSYGQNVLMHSKEVA
                     IIAGLLAAELGLNREIAKRGALLHDIGKGIVTDSDKNHAEIGTEIAKKFGEDTRVINA
                     IASHHNDVEPNCPESVIVQIADAISAARPGARRETLDNYIKRLEDLEAVAESFSGVEK
                     AFAIQAGRELRIIVSNEQVSDDQAKELCKDIAKKIESDLRYPGRIKVTIIRETRIVEY
                     AR"
     gene            12124..12960
                     /locus_tag="PLS88_04415"
     CDS             12124..12960
                     /locus_tag="PLS88_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOU06622.1"
                     /translation="MTANVLMIGDIVGAPGLRALFAILPTLVRKTEADLVVANGENAL
                     KGYGIGPDEIAAMRSYGVDIITSGNHVWERKEAAALLESEAQLLRPANYPKGLPGRGF
                     AYVGGRVSAGQQAIVRASGAAARFEWLVVNLQGRRSMYDIDCPFTKADQLLAAAARDH
                     PNALIVVDFHAESNEEKEALAWYLDGRISVFAGTHTHVTSADERVLPKGTGYLTDLGM
                     TGPIDSVIGMNGDICIRRFITQIPYKMETAEGSSAIMGAIFRIEPKSHRCVEIERVYQ
                     SL"
     gene            13097..13178
                     /locus_tag="PLS88_04420"
     tRNA            13097..13178
                     /locus_tag="PLS88_04420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13131..13133,aa:Leu,seq:gag)
     gene            13285..14472
                     /locus_tag="PLS88_04425"
     CDS             13285..14472
                     /locus_tag="PLS88_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOU06623.1"
                     /translation="MRPKKHALFTLFKHHNANGTTVWYARFWDDATQRYAIARSTGVL
                     AQGKRERHDEAITVAREMASSIQFSSSPLKQLFVDYVESFWTSSSCYVKERALVLKKP
                     LSAYYVKMNHDDVGRHIRPFPGFRKLTLNDLTPGHIRDWMRWAAEKGLSARRINAVLQ
                     SMRVAVRYAVSREELLRDPFAKIAEVKEEIKEKGILTPAEVSLLINRNGNDDPRIYLG
                     ILLGVFCGLRRGEVRGLQWGDVDLNQRIIHVNHNYVDNEGIKTPKCKSMRSVPIPRAV
                     YSAFNAVRTVSPYLASDDFVLFNFLSRDIPVSMNFFRAGLCSVLESIGIKKEAQKLRN
                     ITFHSLRHTFVTLGRLAGISDFEIQALAGHRSSSMMERYSHVCQVIDYGSVCKKLEDA
                     ANQ"
     gene            14564..14890
                     /locus_tag="PLS88_04430"
     CDS             14564..14890
                     /locus_tag="PLS88_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06624.1"
                     /translation="MDSDDFDDAFVLRYKINRYPAKYFRKLRAEGAKNKVIAQMFGIS
                     ELKVRKVINKWPDEKLITVYDYDLVPSRGHHCIYDEELYDEEGHSFGYLDYQLNLEHE
                     YHKGRC"
     gene            15091..15291
                     /locus_tag="PLS88_04435"
     CDS             15091..15291
                     /locus_tag="PLS88_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU06625.1"
                     /translation="MREPNQQIFFLSRKEAAQYLRISLATLVRRLDDGSIPYIKLGKR
                     ILIPVEALERLATSMFTMPGGK"
     gene            15491..15874
                     /locus_tag="PLS88_04440"
     CDS             15491..15874
                     /locus_tag="PLS88_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06626.1"
                     /translation="MSNTIKNEARGLGGLEAGQKGLSSYSINKKKLTIKSKIIRLRQS
                     WHLEIADFERFAIFVSRLFLDFAPIEVRARFIGPPSDTPREISELSETFLLLYALAED
                     KATATDTKYSLTEIWRAAVQLAGGA"
     gene            15874..17955
                     /locus_tag="PLS88_04445"
     CDS             15874..17955
                     /locus_tag="PLS88_04445"
                     /inference="COORDINATES: protein motif:HMM:NF040238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HOU06627.1"
                     /translation="MAEITLPVENILRGIGIELPASVHDRLPASTSSHIEQFLQEPTH
                     SLAADCLFERFAGKIIFERTAPKQGRFWQRQPGGYFEPLDSMLDLASKYLDTAVDEAF
                     EKLKAEAEGATISALFTKAGIARRKARTRDFINGALEFFAAKVLVPNLSARWNEAQEC
                     LPCTDGVIDFTGEEIIARPPRDNEYFKDPLPVKTADILREDIPASFLLFLDEVFPDPE
                     VRRTALECVSLAVANKGSRTFYLWHGSGANGKNTLLDGLKIVLGARVGTIPAGAIIRS
                     HDGSGRFAAAALNGLTFAAVDEVSAPLDTSEIKKLTGNSSFSIEEKGRQAFEIAQRWA
                     LAAFTNRLPSFSPANDSAFTERLIIVPFDSVFYFNDEQKRHYIDMGVPAEWLIQARDR
                     AAIMDGFDTDRAQILRMLIQTYMKVRAAGGRPFEGKRCYQLKQNYIDANDLIADFFRE
                     HFVRADGERMPYMRILELYRDYTGDKNIATRTCIQKLIARFPWMQKTVANSVRYLVNI
                     RERDGNDNEPDTSSEAENSSESQEMLFNVESANMEDTGFSTQVRKNGTLRSTLEKCHF
                     SYINIKTPVFRTTVLENDPSVLSDSDLLLASKTASELLKAANEVGKVPIGKWRNACAA
                     KGMDPQKIAAAENYMQQVGLLDGGYLENIKQANEGDQGIDPPKIRLGRPAAGWPAIFK
                     NNEGDSELGLS"
     gene            18039..18311
                     /locus_tag="PLS88_04450"
     CDS             18039..18311
                     /locus_tag="PLS88_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06628.1"
                     /translation="MDKTEISFEKKPAIVRRLSSLAGIRRELARVYEEAKGAGADSER
                     VQYYRALTFILTSCAEVLRNEKIEKLEDRLQALEAAKEEAEEGTND"
     gene            18304..18588
                     /locus_tag="PLS88_04455"
     CDS             18304..18588
                     /locus_tag="PLS88_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06629.1"
                     /translation="MTEMERRLKKIETAGLNASAAAALYQKFRNAKDPKRRERLYNAY
                     LAADPARKCEKSAEIIEKRVRMWQSRDRSVDEKLIANSDWRIIGDGKEGK"
     gene            18830..20434
                     /locus_tag="PLS88_04460"
     CDS             18830..20434
                     /locus_tag="PLS88_04460"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HOU06630.1"
                     /translation="MTPDINGQKGAFLMDAKKEQERAELHRTIWNIANQLRGSVDGWD
                     FKQYVLGMLFYRYISENLTSYINRGEREAGNPDFDYAKLTDEEAEPARMDLVQTKGFF
                     ILPSQLFENVRQRAASDENLNETLENIFRSIEASTIGGPSEANFKGLFDDIDVNSNKL
                     GPTVAKRNERLVKLLDEIGNMKLGDYQDNTIDAFGDAYEFLMSMYASNAGKSGGEFFT
                     PQEVSELLTQIALAGKTEVNKVYDPACGSGSLLLKAAKILGKENVRQGFFGQEINLTT
                     YNLCRINMFLHDIDYDKFDIAHGDTLTDPKHWDDEPFEAIVSNPPYSIRWEGDANPLL
                     INDPRFSPAGVLAPKSKADLAFIMHSLAWLATTGTAAIVCFPGVLYRGGAEQKIRKYL
                     IDNNYVDCIIQLPDNLFFGTSIATCIMVLKKSKTDNSTLFIDASKECVKITNNNKLTQ
                     ENIETILSAYTARGNKDHFARLVPNAEIAAQDYNLSVSTYIDQKDTREVIDIKKLNKE
                     IEKIVAREQELRDEIAKIIAEIEGQA"
     gene            20431..>20961
                     /locus_tag="PLS88_04465"
     CDS             20431..>20961
                     /locus_tag="PLS88_04465"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOU06631.1"
                     /translation="MSRLDELIAELCPDGVEYRLLAELEDAKLIKLGRGNVISKKEIR
                     EIPGNYPVYSSSAVGNGEIGSYGKYMFNDERITWSIDGGGRFFYRPAHRYSITNVSGF
                     IKILSKERINTKYLFYALINEWRKKIFNYTHKAHPSIIRNEYKIPIPPLPIQQEIVRI
                     LDTFTELEAELEAELEA"
     assembly_gap    20962..21061
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21169..>23655
                     /locus_tag="PLS88_04470"
     CDS             21169..>23655
                     /locus_tag="PLS88_04470"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOU06632.1"
                     /translation="MSQYNIVVATDESTVVSEYVPEYSAGNAYQSEAELEQAFIKQLQ
                     AQGYAYLPIHHEEELITNLRHQLELLNSYTFTENEWNRFFTNNLASKNEGIIEKTRKI
                     QTDHVQILQRDDGTTKNIYLLDKKNIHNNHLQVINQFEETQGQHKTRYDVTILVNGFP
                     LVHVELKRRGVAIREAFNQIKRYQRESFWAASGLFEYVQIFVISNGTHTKYYSNTTRS
                     YHIKETSEAERSQSKKTSNSFEFTCYWADANNKPIADLMDFTKTFFAKHTLLNVLTKY
                     CVFTTEDLLLVMRPYQIAATERILSKIEIASNYKKTGTIEAGGYIWHTTGSGKTLTSF
                     KTAQLASMLPYIDKVLFVVDRKDLDYQTMKEYDRFQKGAANSNTSTRILQRQLEDPSA
                     KIIITTIQKLDRFISLNRTHDVYKKHVVIIFDECHRSQFGDMHRKIIKSFKNYHLFGF
                     TGTPIFAVNASNNGNPLMKTTEQVFGDKLHTYTIVDAINDGNVLPFRIDYINTVKMKD
                     RVEDKSVSAIDTEKALEAPERIREIVCYILDHFDQKTKRNSFYTLKGQRVAGFNSILA
                     VSSIPMAMKYYTELKRQLAERGRELMVATIFSYSANEADPEDVFPDENFDTDSLPPTA
                     REFLGSAIADYNAAFNVNFDTSADKFENYYKDLSLRVKKREVDLLVVVNMFLTGFDAT
                     TLNTLWVDKNLRLHGLLQAFSRTNRILNSVKTYGNIVCFRNLQKETDEAIALFGDKDA
                     GSIVLLKDYDSYYHGYNDEHGKHQPGYIELIEELQTKFPLGQAITGEARQKEFIKLYG
                     AILRLKNILTAFDNFEGNEILAERDFQDYLS"
BASE COUNT         6614 a         5354 c         5728 g         5859 t
ORIGIN      
        1 tctattatgt gcatttttta gaagttcacg ggttcttttt tgttttttcg aacgagcgga
       61 tggttgctca aaaatgaaca cttcatgacc tttggccaga agagcgccaa ttacacttgt
      121 tccaacattt ccaaatccac cagtcactaa gattcgcata gttctcctca catttgattc
      181 tatcgcatac tattataatg aatgaacgtt cattcagcaa catagaactt tttaaaattc
      241 gaggttattg ctcattgacc tgctcatagg gctttcgtta cacttgccaa gctatgatca
      301 caatcgatga actgcgcaga aaatacattg aattcttcaa ggctcgaggc catgctgaga
      361 ttagcggtaa gtcgctcatc ccagagaatg atccaacggt actttttaca actgcaggca
      421 tgcatcctct cgtgccatat ctgcttggcg aaccacatcc agctggtaag aggctgaccg
      481 attatcagaa atgcatccgc accggcgaca tagatatggt aggggacacc agtcacctta
      541 cttttttcga aatgctgggt aactggtctt tgggagatta cttcaaaaat gaggccattc
      601 attggtccta tgagtttttg acaagtccgg attatctcgg tatagatcct gtcaagcttt
      661 ccgttactgt atttgctgga gatgagtcgg tgccgcgaga cgatgaatcg gcttatttat
      721 ggaaaaaggt tggcatccct gaggagagaa tctttttcct tccgcgcgaa gacaattggt
      781 ggggtccagc tggagaaacc ggcccatgcg gcccggatac ggaaatgttt gtcgatacag
      841 gtgcgcctgc ctgcggtcca gattgcaaac caggctgcca ttgtggcaag tactttgaaa
      901 tctggaacga tgtttttatg cagtataaca agaaggcaga tggaagctat gagctccttg
      961 ctcgcccttg tgtcgatacc ggcatgggaa ttgagcgtac tgttgccatg ctgcaaggga
     1021 aaaagtcggt ttatgaaatc gaggccttta ctccaatctt gaaggtactt gaatctataa
     1081 ccggcaagac ttatggagtt tctagcagcg atgccgaaat tgatcgaagt tttcgaataa
     1141 tagcagatca tattcgtgca gcagcattta ttcttggaga tcctaaggct gttttgccat
     1201 ccaatattgg agctggatat gtattacggc gcttgatccg tcgcgcggtt cggcatggaa
     1261 aaaagcttgg catcgaggga cttttccttt ccaggcccgc ccaagccgtc atcgatatct
     1321 acaaagagcc ttatcccgaa ttgcttgaga atgccggtcg tatttttgaa gagctcgagc
     1381 gagaagagaa aaaatttctc gagactctcc agaaagggga acacgaattc gagaaaatct
     1441 tgccaaatct tctaaagaat ccagacaaaa caatgccagg caggctcgct ttcaagctct
     1501 atgacaccta tggctttccc atcgaactga ccgaagaatt agctgcggaa catgagctca
     1561 aggtagacaa acagggcttc gaggaatcct tcaaaaagca tcaagaaatc tcaagagccg
     1621 gaagtgaaca ggttttcaag ggaggattgg cagacaattc ggaaattgtt acgcgctatc
     1681 acagcgcaac tcatcttttg cacaaggcat tacgcttagt gcttggcaat catgttgctc
     1741 agaaaggttc gaacataacg gccgaacgct tgcgcttcga tttttctcac ccagcgccga
     1801 tgacaccaga gcaaatcgct gctgttgaac gcattgttaa tgaacagata gctcgagatc
     1861 tgcctgtcac gatggagatg atgcctccgg aagaagcaaa ggctgccggc gctattgcgc
     1921 tttttggtga gaaatatgag cctattgtaa aagtctatac gatgggcgat ttttcaaagg
     1981 aagtatgcgg tggtcctcat gtttcgcata ctggcgagat aggaaaattc aagataatca
     2041 aggaacagtc ctcgagtgct ggagtgcggc gcatctacgc tgtgctggaa taggtttata
     2101 cctattctgg tctcattttt ttgagtgcct ggacggctac gcggtcatcc tcaggaacaa
     2161 ggcgcaaaaa ccatgagcct tcgcatttaa ccctttgcca ccatacttcg cgtatagctt
     2221 cttcaagaag cgactttaag cgccatacct cttcgtcata atacatatct tgctgacaaa
     2281 atatatactt tcctatgtca agagaaaata aagaagtcct tcctagattt atatcattgg
     2341 gagccattgt ttctatatat ttgttttctg ccaagcggta tactgcggat ggcttgagat
     2401 cttcaaatac tttgggtcca tcatcggtca ttgtggaaag gcagttttct tcgaactcaa
     2461 gcaacaattc actcttatta gagagaaaat ccgataaagt ctttgcttcg acgatctgag
     2521 gttcctctat atggacgtag tcgaggcttt ggcatagatg cagagttttg agctcaattt
     2581 ccatatccgt attctcgaat cctttatcta ggattgtcaa gagacaagca agattcgaaa
     2641 gccttccgag atgcatagag gcgcaaaaaa tcgcgcagat tccataatcc cgctggaaat
     2701 cttcgcgaat tgatgataga ctatttcgaa atgtccttgc ttgaacgaat ctcagggcca
     2761 gccgacctgc agggcttatc tatgcccgat ttgcggcgcc tctcatcaga aattaggcaa
     2821 agcataatcg aaacagtaca gcgcaatgga ggtcatcttg catcgaatct tggggttgtt
     2881 gagcttacga ttgcgcttca tcgtgtattt caatcgccat acgatgcaat agtctgggat
     2941 gttggacatc agtgctattc tcataagatc ttgactggtc gtgcagctcg attcgtcacc
     3001 cttagaagaa aagggggctt atcgggattt cccaagcgaa aagagagcaa acacgatgtc
     3061 tttgatacag gacattcttc tacagccatc tcttccgctc ttgggcttct tcaagctcgc
     3121 ctgagcacag gccatgctgg cagggtgatc gctgttgttg gcgacggagc gcttaccgca
     3181 gggctttcta tggaaggact cagcaatgca ggccaattgg gtttgcctct aatcattgtc
     3241 ctgaatgaca accgaatgtc tatctctcac tctgtgggct cgatgtcgag atatctttcg
     3301 aagctgtcgg cgtcggtacg ataccagtca ttgcgatcgc gtgtagatgc ggcggtcctg
     3361 agaatacctc gcattggaga ggcgctcttt tcccttataa atagaaccaa gcgagccata
     3421 aaagccattt ttttcaaaga gaatctcttc gttgattatg gattcgaata tgttgggccc
     3481 atagatggac acaatcttgt ctcattgata gataccttcg agcatgtccg caagctgaat
     3541 aggccagtcg ttgttcatgt cgtgacaagg aaaggaaaag gactcgaaaa agcagaagag
     3601 gatccggaga gttttcatgg tcttggtcca gcttgccccg atatacctgg gcctggatcg
     3661 ataagccatg gagaaagttt tacatcgtgt tttgccaatg ctctcataag atgtgcggag
     3721 aaagaaccac gcatggttgc tataaccgcc gccatgtctt cgggcacggg ggtttcaagg
     3781 cttgcagagc gctatcccca tagggtatat gatgtgggta tcgcggaaca gcatgctgtg
     3841 gcatttgcag ccggtcttgc acagggggga ttgcggcctg tggtggctgt gtattctact
     3901 ttcatgcaga gagcgttaga tcaagtgttc caagaagttg cgctggcgaa acagccggtg
     3961 ttgtttgcac tcgatcgtgc tggagccgtt ggcgaagatg gcgagactca ccagggaatc
     4021 tacgatattg tcctcttcaa ggctatgccg aatttattgt tttttgctcc tgcggatggg
     4081 gaggaattgt cagccttcat ggatctagcg attactttgg atctgccttg catgatgaga
     4141 tatcccaaag ctttctctca ttcttcgact cttccgatag cacctcttga gattggaaga
     4201 ggcgttttta tgcgcgatca tgatgatgca agaatactta tattggctgt tggaccgctc
     4261 tcctatattg cagccgaggt atcggatgaa ctagagacaa aagggatcgt tgcggatgtc
     4321 tacaatgtcc gattcatatc tccgatcgac gaagaacacc ttgtcgatat ctgttctcga
     4381 tatgatggag tcttgacaat cgaggatgga gttttgaatg gtggtcttgg agagtctgtt
     4441 gcaggaatac tcatgcgtaa aggcatgcag atcaagattt cttccttggg ttttggatcg
     4501 ataccgcttg ctcaagcaac tagagaagaa cttctttcta gcgcaggcct agactcaaag
     4561 ggcatacaaa aagctctgta cgatctcttg aatagcctcg atctatcgaa gcagcaatta
     4621 ggcacaggaa tcccagcgaa cttgtctgga agattatcga ctcctagcga gattcagcat
     4681 gcgcattagc gcaaaaagaa tgcaagcgaa aaatcactca agcaagctaa gacttccgaa
     4741 tgggcgttca ctggaaatag gtagtgcccc agaacatttg ccttgcataa tgggtatcgt
     4801 caatatcaca aaggattcct ttttcgaagg gagcagaagg cttgagccaa aagccgcaat
     4861 cgagacagct ctcgacatga taaggcaggg tgcttctatt atagactttg gcgctgagtc
     4921 tacgagaccg ggttccaaag agatcgatcc aaaagaggaa ctatcaagaa ttttgcctgt
     4981 tttagaaggc ttcagggcag taagcgatgc cgtaatttcg gtcgacacac gtcaccccaa
     5041 agtcgcagaa gcagcacttg ctgctggagc agatataatc aatgatatcg aagcattaca
     5101 aaggccaggc atggccgaag tcgttgcaag gggcagggca gcagcgattc ttatgcatat
     5161 gcaaggaaca ccccagaaca tgcaacaaaa cccttcctat gaaaactgcc tcgaagaagt
     5221 ccgctctttc cttgaaggtg ccgctgacta cgctatcgag gctggtatat ccccagacgc
     5281 gataatcctc gatccaggta tagggtttgg taaactcaag gagcacaata ttgcagtttt
     5341 gcagggtatt gaacgcatag cttcgcttgg tttccctgtt ctgattggcc tttctcgtaa
     5401 acgactcata ggagaactta ctggtaagga tgtcgccgat cggctcgcgg gcagtctagg
     5461 cgctgcttta gcagcttggc taaatggcgc tcagattctt cgagtacacg atgtagcaga
     5521 aactttggac gcacttcgag cctttcatga ggtgatacaa tgaatgggcc gacttttatc
     5581 caatttttta ctgtatatgt gcggcccgtg ctcgatgtcg gcattcttgc ttttcttatt
     5641 tacaagacct atcagcttct tgtaatgacc gatgcggtct atctaattcg cggtctgcta
     5701 attcttctcg ccatatatgg gatatctttt tttctaaagc tctctacagt cacatggatc
     5761 atgaatatac tagctccagg attggtgata ggccttgcga taattcttca gcctgagcta
     5821 aggcggattt tcattcagat tggacgccaa tcgattttcg gaagaaagtc tatggctcgc
     5881 agttcttgga ttgaggccgc agtatctgct tctgtttatc ttgccgaaaa aaggcgcgga
     5941 gcgatcatcg tcatggccag aaaagtaggt ctagcctctt acatcgaaaa aggtattcca
     6001 ctcgacgcaa tcatctcaac cagcttgctt atatccgtgt tcgaacacga caacccattg
     6061 catgatggcg ctgcgatcat ctcgcagggc aggctcgcag cagcgggctg ctttcttcct
     6121 ctttccgatc agcaagaaat tcgcaagact tttggctcaa ggcacagagc cgcgcttggt
     6181 attgctgaag aatccgatgc ggttgtactt gtggtctctg aagaaaccgg aactctctct
     6241 ctcgcttatg attcaaagct gtattatgat ctttcttccg aggaagttct ggaacgcctt
     6301 catatcctct tgtcagagca aaatctgaga agagagggaa atgaggtcgc caatgagcct
     6361 tgattccaag atttctaaat tgctggagaa ttggcccgcc aaggtcatct ctcttgttat
     6421 ggcgattttt attatgtttc tttacaacct cactcgtctc gagcagcgtg tcataatagt
     6481 gccgctaaac atctctgaag ggcacagcta tgtgcctgca acagaatatc caaagactgt
     6541 ccgtgtgata atccgaggtg accgtgagca aatctataga gttcgcgagt cagatatcgt
     6601 tgcgagcctc gatttggcac gttatagttc tgaaggcgta ttcagggttc cagtaaagct
     6661 tgaaaggcga ggcgatgctt tgaatatcga tcccctagag ctaaaggcgg agccctcaga
     6721 aattcccatc agttttgagc gcttggctgc aaagcgcgtg gccataactc ctaccttcaa
     6781 aggttttcta gaacagggtt atgtcttgat ttcctatgaa atcttgccat cagagatatc
     6841 tatagaaggg ccaacgagtc tggtgagcag tatcaaggat atttctaccg atataataga
     6901 acttagcggc aagaccagcg atttttctct gagcgtgcca cttgtcaagc cttcggatct
     6961 cataaggctt gtcgacgcaa acacagtaaa gttctcggca aagattcaga tacaggaaag
     7021 gcgagttaca ttaaagaatg tgcctatata cattagcaac cttgatccat cgcttacgat
     7081 ttccgatcag ctcccttctg gaaagataac ccttgtacca caacctggac aggatgagac
     7141 tttagcaagc gacgccagcc ttcaggctga tttcaagggc atagtaaagc caggtcttta
     7201 ttctataccg cttactgtaa ataccccaga aggtttcgaa gtcgaagcat acgaacctct
     7261 agcgctcaat gtccgtctgc atagtgcgcc cgttcttccc acttttcatt gaaatttcaa
     7321 ggagcgatcc atgatttcag gaattggaat cgatattgtc catgtcgatc gcattcgtcg
     7381 atggatggat gaaccaggaa ttctacagcg cttcttcaat ccagcagaga tcgaaacagc
     7441 gagctttcgc aaaaagggta tggctctttc ccttgctgct cgcttcgccg ctaaagaagc
     7501 ctttggtaaa gcactcggtt ctggtcttgc caactttgcc ttaaaggaag ttgcggtagt
     7561 aaacgatagt ttgggtcgcc ctataatgcg cttagaaggt aatgcacgcc gcgagtttga
     7621 gcggcacggt ggaggaaaga tccacatctc gatgactcat gaaggagaca atgctgttgc
     7681 aatcgtaatt ctagaaaagg aaaactagaa aatgctagaa aatagtacaa cgcatgaggt
     7741 tcttgcctgc acatcaaagc cctcattgcg cgctatcaaa ctcgtccttg cttacgatgg
     7801 cagcgatttt tccggttggc agaaacagaa aaatgggcgc tcggttcaag aggaactcga
     7861 aaaagcactt tcgataatgc ataaacacga agtgcgagtc gtaggaagcg cccgcactga
     7921 tgcaggcgtt catgctatgg gacaagccgc aagcttctat acggatatcg cttcgattcc
     7981 agccaatcgt tttgtattgg ccttaaataa actcttgcca cgcgatgtgc gcgtgctctc
     8041 ttctgaagag gagaaacctg attttcatgc cagattcgat gcttctttga ggcgatatcg
     8101 gtattttctg agctgcggag ggacgcctga tccatttact cttcgctatg ctcattttat
     8161 tccttactat ccatgtctta ccattctgaa tgcgatggct tctgtggtgt taggggagca
     8221 cgatttttcg acttttgcct ctgcacaaga taagagcgaa tcaaggtttc gcttcataag
     8281 cgagtcagtg tttttcttag agagagactt gctcgtctat cagatagcag gtaatgcttt
     8341 tctatggcgt caggtgcgct ctcttgtggg gacttttctt gaactcgaaa agaaagcaaa
     8401 ggatcctggc gaagcagaaa tcttgatgca cgaagtcctt gaatccaaag atcgaagtag
     8461 ggcagggcct acggctccag cctgcgggct tttcctctgg aatatcgaat atggagagag
     8521 agtccatggc catacccgaa agcagcacaa taaaaaagaa agcagcaagg cagaccccat
     8581 ttccccagtt tgggaaggga tagccaatcc atcgatgccc atgagagccg ataggtatct
     8641 ctcagagata atcaagctca tgactcgttc tcagctaaag gctcgaaatg ctcacctctt
     8701 ttgtaatgga gtagaagtaa aattttctca taagcttaaa agcaaagatc gtttgcgcct
     8761 tgaatggaca gaagctcctt cagggcaaat agagcctgag gctataccat tggagattct
     8821 ttatcaagat gcagatgtct atgtaatcga taaaccgcag ggaatggtca cccatcctgc
     8881 ggctggcaat tggcatggca ctcttgcgaa tgcggtacta tggctggatc acgaagctct
     8941 acaaaatatt caagttcaag attctttctc gatttcttct cctgctcgcg ctggcattgt
     9001 ccatcgtctt gataaagaca cttctggtgt aatcatcgta gcacgaacag ccactgccca
     9061 ggaattctta tcaagtcagt ttagagaaca ccaagctaga aaagaatatt ttgcaatagt
     9121 aaggggtatt ccgcctgagc caaaaggcag aattgataca tggatagcgc gcaaccaaag
     9181 agatcgcaaa aagtttacag taagcgaacc cggccgcggc aagcatgcac ttactctata
     9241 tagagtatgc agagcatggg aggcaaattg ctacatcgat gatcgattgg tcgatgttgc
     9301 ttctaaagga gcaaagcctt ctatttactc gttacttgct ctctatccca gaacagggcg
     9361 aacccaccaa ctccgagtac actgcgctaa tattggttgt ccaattctag gagatcctat
     9421 ttatggtaag aaagatgaaa tctttccaga agctacgctt atgctccatg ctcatcggct
     9481 caaaattcgt ctgccttctc gatcagaacc ctcggtcttt tcgtctcctt ttcctactag
     9541 attcagagat atcatttctt acctggacaa gcatggccat aatataaaag tcacagatta
     9601 aaaatcacaa tgtcatattt gagtatttct taatatcgaa aaggccttat gaaggcctta
     9661 gattatagaa ttataaaaaa accggtacaa gtttatgctt gcaccggttc tatttaccac
     9721 aacatagccc ccatatggct ttgtgaccta aggctggctt tgtggttctt gtgaaagact
     9781 cgcgctagcg gcttttaggt gcgagtcttt attgtcttag tatctgcggc ctcggtaacc
     9841 gccggtatct tccctttcgt agcgtggctt ctcctgcgct tcattcacgc ggagctgcct
     9901 acccatgaat tcctgaccat tgagcgcggc gattgcggcg ctggctgcat cgggattatc
     9961 catctcaacg aagccaaatc ctttcgcttt gcctgtgaat ctatcgacaa ttatattcac
    10021 agatgagaca tcgccatact gagcaaacaa atcttgaagc tgacgctcag aagtgttgta
    10081 attcatgtta ccgacataaa ttttcttgga catttcggaa aaacctcaaa tctaggcctc
    10141 gtcctctcga cgtaggccgg tttgattcgc ctagggcgaa tctgtagcga aatcctgaag
    10201 gcacatattt gcctccaaag tcttcagagg agaaatcaga gaactgtttt gattattttt
    10261 ggtctttccg aatgaaaaac aaggaagaga aaaaccggca cttgcgtatg ccgccatcag
    10321 caagccagtg ttacgaaagg aaaaacaatg catgaaacag ccaattccaa cctgaaaacc
    10381 tatggaagac gattgtccct ccgcagtttt cacttatatc tgcagaattt aaaccatttt
    10441 ttaaagatcg tcaatagaca cagacatatt tcagcagcaa aaaagccatt ataagccaag
    10501 agttataaaa atatggacaa acttgacaaa gacccctcca tcgctataat caaaagtacg
    10561 gcatattatt taatagggag ggcttatggc accattagtg gggtacatcg tccttcctct
    10621 tgccggtctt attctaggat ggatgatccg ctggctatat gccagatttc ggctctcttc
    10681 cgctgagagg gaagcggagc gtgttttgca ggatgcaata aaagaggcgg aggccaagaa
    10741 aaaagagatc ctggtagaag caaaagatca gatcattcga gaacgaaacc agcaggagag
    10801 ggaactgcga gataggagaa tggagctgca gaaatatgag cggcgagttc tcctcaagga
    10861 agaaaatctc gatgcaaagc ttgcatctat agaacgcatt gaggcagatc ttaaaaacag
    10921 agaaaacaaa atagcagaaa aagaagaata tctagcagga caggaagagc gatacgctgc
    10981 ggagctcgag cgagtatcgg gctttaccgc cgaggaagct aaaaagctta ttatacaaaa
    11041 tatggagcaa gaagcacgca gagatgctca gaccttgatc aataaaatcg atcaagaagc
    11101 acaactgtcg gcggagaaga aagccaaaga cattctcatc accacaattc aacgcatcgc
    11161 aactgaatcg actgcagaat ctacaatttc ctctgtgagt cttccttccg atgagatgaa
    11221 aggtcggatt atagggcgcg aaggccgcaa cattcgcacg ctagaaacat tgaccggcgt
    11281 cgatatcata atcgatgata caccagaagc tgtggttata tcatgttttg atccaattca
    11341 gcgcgagatt gcgaagattt cgcttgaaag gctcattgca gacggccgca tacaccctgc
    11401 acgcattgaa gaaatgatac agaaggtgac ccgtgagatc aatcaaaagc tctacgacga
    11461 aggcgaaaag gttgttttcg atcttggctt gcacaacatg gctccagagc tgattagagc
    11521 tgttggaaga ctctactacc gcacgagtta tggacagaac gtactgatgc actcaaagga
    11581 agtggctatt attgcaggac ttctagcagc ggagcttggc ctaaaccgcg aaatagcaaa
    11641 gcggggtgcg ctgcttcacg acattgggaa gggcattgtt acagatagcg ataagaatca
    11701 cgcagagatc ggcacagaaa tcgctaaaaa atttggtgaa gatacgcgtg taatcaatgc
    11761 gattgcatcc catcataacg atgtagaacc caattgtccg gaaagtgtaa ttgtgcagat
    11821 tgcagacgct atttcggctg cccggcctgg tgcaaggcgt gagactctgg ataactacat
    11881 aaaacgtcta gaagaccttg aagctgtagc agagagcttt tctggtgtag aaaaggcctt
    11941 tgctattcag gcaggtcgag agcttcgtat cattgtaagc aatgaacaag tttcggacga
    12001 tcaggccaag gagctttgca aggatatcgc caagaaaata gaatcagatt tacgctatcc
    12061 tggaagaata aaagtcacaa tcataaggga aactcgaata gtggaatacg cgcgatgagc
    12121 tctatgactg ccaatgtact tatgatcggc gatattgtcg gagcgcccgg ccttcgggcg
    12181 ctctttgcta ttttgccgac tttagttaga aagaccgaag cagatctcgt tgtcgcgaac
    12241 ggcgaaaatg cacttaaggg ttatggaatt ggcccggacg aaattgcagc gatgcgttcc
    12301 tatggtgtcg acatcataac gagcggcaat catgtttggg agagaaaaga agcagcagct
    12361 ttactcgaaa gcgaagctca gctattgcgt ccggcaaatt atccaaaagg attacctggc
    12421 cgtgggtttg cttatgtagg tgggcgagtc tctgccggac agcaagccat tgtccgagcg
    12481 agtggtgcag ctgctcgctt cgaatggctt gtggtgaatc ttcagggccg ccggagcatg
    12541 tacgatatag attgtccctt tactaaggct gatcagctgc ttgcagcagc cgcgcgcgat
    12601 cacccaaatg ctctcattgt cgtcgatttc cacgcagagt ccaatgaaga aaaagaggct
    12661 ctagcttggt atttggatgg acgtatctct gtatttgcag gaactcatac ccatgtgacc
    12721 agtgccgatg aaagagtgct acccaaaggc actggatatc tcaccgatct tggaatgacc
    12781 ggcccaatcg attcagtgat aggcatgaat ggtgatatct gtatacgccg ttttattacc
    12841 cagataccat ataagatgga aacggcagaa gggagttccg caataatggg ggcgattttt
    12901 agaatcgaac caaaaagcca tcgctgtgtg gaaatagaga gagtctacca gagcttataa
    12961 atatcgagag tgcaaaaaac tagcagattg ctttcattat agatatttct tacggttatg
    13021 atgtctattt ctcaaggcct cttgacattt gagttcaggc aggtatatat aaacgagcaa
    13081 ataaacgaat aagattgcgg cgatggtgga attggtagac acgctagctt gaggtgctag
    13141 tgccgagagg catggaggtt caagtcctct tcgccgcaaa tattgaatta acaaagaatt
    13201 agcctacccg aaaacgggcg gcatatcatg cgactatcca gaagtgacac ttgaaagtga
    13261 cactttcatg gaaaaggaga ggatatgcgc cccaaaaaac acgctttgtt cacacttttc
    13321 aaacaccaca atgctaatgg gactactgtc tggtacgctc gattttggga tgatgcgaca
    13381 cagcggtatg ctatagcccg ctcaaccggc gtactagctc aaggaaagcg cgagcgccac
    13441 gatgaggcta tcacggttgc tcgtgaaatg gcttcatcaa tccagttttc tagctcgcct
    13501 ttaaaacaac tgtttgtgga ttatgtggaa tcattctgga cttcttcaag ctgctatgtc
    13561 aaagaacgcg cgcttgtatt gaaaaaacct ctttcggcct attatgttaa gatgaatcac
    13621 gatgatgttg gtcggcacat tcggcctttc cctggattta gaaagttaac tcttaatgac
    13681 ctcactcccg ggcatattag agactggatg cgatgggctg ctgaaaaagg attgagcgca
    13741 cggcgaataa atgctgtgct gcagtctatg agagtggcag ttcgatatgc tgtatctcgc
    13801 gaagaattgt tgcgggaccc atttgcaaaa atagcagaag taaaggaaga gataaaagaa
    13861 aaaggaatat tgacgcctgc agaagtatca ttattgatca atagaaatgg aaatgatgat
    13921 cctcgcattt atctgggaat actactaggc gtattttgcg gattgcgaag aggtgaagtc
    13981 cggggcttac aatggggaga tgttgacctt aaccagagaa tcattcatgt aaaccataat
    14041 tatgttgata atgaaggaat aaaaacgcct aaatgcaaaa gcatgagaag tgtgcctatt
    14101 ccaagagcag tttattctgc ttttaatgca gttcgtactg tttccccgta tcttgccagc
    14161 gatgactttg tactattcaa ctttttgtct cgagatattc cagttagtat gaattttttc
    14221 cgggcagggc tttgcagtgt acttgaatcg attggtatta aaaaggaagc gcaaaaattg
    14281 agaaacatta ctttccatag tttgagacat acttttgtaa cgctagggcg cttggcaggt
    14341 attagcgatt tcgaaattca ggctcttgct gggcacagga gctcctcaat gatggagcga
    14401 tatagccatg tttgccaggt aatcgattat ggttcagttt gcaagaaact tgaggacgcg
    14461 gcaaatcaat gaatagtaat tgaggaaaag cttttatcaa gaatgtcata gattacaatg
    14521 gagcttcttg cattgcaatg agattttgta gtatactaaa catatggata gtgatgattt
    14581 tgatgatgcg tttgtattga ggtataaaat aaatagatac ccagcaaaat attttcgcaa
    14641 gctccgagca gagggggcta aaaataaagt cattgctcaa atgttcggca tttcggagct
    14701 caaagtgaga aaggtgataa ataaatggcc ggatgaaaaa ctcataacag tgtatgatta
    14761 tgacttggtt ccatcgaggg gtcatcattg catttatgat gaagaacttt atgacgaaga
    14821 aggccatagt tttggatatt tagattatca attaaattta gagcatgaat accataaagg
    14881 gagatgttga ttttaaaaag ggattcatag ccaattctag ttggctaaaa taaatatcac
    14941 gttaaggaga acaggagtaa ggacaactga tccctgcctt aacgttgctc tggttccgta
    15001 tggggccgtg aagagctgaa ggcgccctgt tttatcctgt gcgccaacat tctctcccca
    15061 aagtgagggg aggagtgttg gaggcgcacc atgcgtgaac caaatcagca aatttttttc
    15121 ctttctagaa aagaggctgc tcagtacctg cgtatcagcc tcgcgaccct tgttcgtcgt
    15181 ctagacgacg gatccattcc ttacatcaaa ctcggtaaac gtattctcat accagttgaa
    15241 gcactagaac gactggccac tagcatgttc acaatgccag ggggcaagtg atgaataccc
    15301 ctaccataga tgcagatgca ctctttgcaa ttcttaagcc ggaagttgaa aagctttgct
    15361 acaacgctcc cctatttggg gaaattactc ttaaggctac aatccaccag ggtaatatag
    15421 cgaaaatatc cacgggaata gaaatatctt gcaagattga cccccgctta ttgaacagag
    15481 aggggcgaaa atgagcaaca caattaaaaa tgaagcccgc gggctaggcg ggcttgaagc
    15541 agggcagaaa ggcttatctt cttatagtat aaacaaaaaa aaactcacta tcaagagcaa
    15601 gatcattagg ttgcgccaat cgtggcacct tgaaattgcg gactttgagc gattcgcgat
    15661 cttcgtttcg cgattgtttc ttgattttgc gcctatcgaa gtgcgggcca gatttatagg
    15721 gccgccctcc gacacgccgc gcgagataag cgagctttca gaaacttttt tgcttctgta
    15781 cgcgctggca gaggacaagg caacagctac cgacaccaaa tattcgctta ctgagatttg
    15841 gagggctgct gtgcagcttg cggggggtgc ataatggcag aaatcacact tcctgtcgaa
    15901 aacatccttc gcggcatcgg tattgagctt ccggcttccg tgcatgatcg cttgcctgca
    15961 agtacttcaa gccatatcga gcaattcctg caagaaccga cccattcctt ggccgctgat
    16021 tgcctgttcg agcgattcgc cggaaagatt atttttgagc gtaccgcacc gaaacaaggt
    16081 cgtttctggc agcgacaacc tgggggatac tttgagccgc tcgactcaat gcttgatctt
    16141 gcttcaaaat acttggatac agcggttgac gaggcgttcg agaagctaaa ggcagaagct
    16201 gaaggtgcga ccatatcggc gctgtttacg aaagcaggca ttgcacgaag gaaagcgcga
    16261 acacgcgatt ttatcaatgg cgcgcttgaa ttctttgcag cgaaagtact tgtaccgaac
    16321 ctttcagcgc gatggaacga ggcgcaagag tgcttgccat gcaccgatgg cgtgatcgac
    16381 ttcaccggcg aagagattat cgcacggccg ccgagggata atgaatactt caaagatccg
    16441 ctaccagtaa aaacagccga tatcttgcgc gaggatatcc ctgcaagttt cctgctgttt
    16501 cttgacgaag tgttccctga tccggaggta cgccgaactg cgcttgaatg tgtgagccta
    16561 gccgtggcaa acaagggatc gaggacattc tacctatggc atggatcggg cgcgaatggc
    16621 aaaaatacgc tactagatgg gctaaaaatc gtactggggg cgcgtgtcgg cacaattccg
    16681 gcaggtgcga ttatccgctc gcacgatgga tcggggcgct tcgcggcggc tgcgctcaat
    16741 gggctgacct tcgccgcggt tgatgaggtg agtgcgccgc ttgatacgag cgagatcaag
    16801 aaactcacag gaaattctag tttttctatc gaggaaaaag gccgccaggc gtttgagatt
    16861 gcgcagcgct gggcacttgc agcgtttaca aaccggctgc cgagcttctc accggcaaac
    16921 gattcggctt tcaccgagcg cctgattatc gtgccgttcg attccgtgtt ctattttaat
    16981 gatgagcaga aacgccacta tatcgacatg ggcgttcctg ccgagtggct tatacaggcg
    17041 cgagaccgtg cggcaattat ggatggcttc gataccgacc gtgcgcagat tttacggatg
    17101 cttatacaaa cctacatgaa ggtacgggcg gcaggcggca ggccttttga aggcaagcga
    17161 tgctaccaac tgaaacaaaa ttacattgat gcaaacgacc tgatcgccga cttctttcga
    17221 gagcattttg tacgggctga cggcgagcgc atgccgtata tgcggatcct tgaactgtac
    17281 cgcgattata ccggcgacaa gaacattgcg acgcgaacct gcatacaaaa gttgatagcg
    17341 cggttccctt ggatgcaaaa aactgttgcg aacagtgtgc gatatctagt gaacatccgt
    17401 gagcgtgatg gcaacgataa cgagccggat acaagcagcg aagcagaaaa cagcagcgaa
    17461 tcgcaagaaa tgttattcaa tgtggaaagc gcaaacatgg aagataccgg tttcagtacg
    17521 caagtacgga agaacggtac tttgagatcg acattagaaa aatgccattt ttcctatatc
    17581 aatatcaaaa cccctgtttt ccgtactacc gtactagaaa atgatccgag tgtgctttct
    17641 gatagtgatc ttcttttagc aagtaaaacg gcaagtgagc ttttgaaggc tgcaaacgag
    17701 gttggcaaag taccgattgg aaagtggcgc aatgcatgtg cagcgaaggg catggatccg
    17761 caaaaaatcg cggcggcgga aaactacatg cagcaggttg gccttttgga tggtgggtat
    17821 cttgaaaata tcaaacaggc taacgagggc gatcaaggta tagatccgcc aaaaatacga
    17881 cttggcaggc ctgccgcggg ctggccggca atttttaaaa acaatgaagg ggacagcgag
    17941 cttgggttat cctgagcgca aaaacaaaga aatgcacgca aaacggcgtc aaatcgcgca
    18001 aattgaaaac gagaaaacgc cgaggagggt tgaaaaacat ggacaaaaca gaaatctctt
    18061 ttgaaaaaaa acctgcaatt gtgcggaggc tttcgagcct tgcagggatt aggcgggaac
    18121 ttgctcgagt ttatgaagaa gcgaaagggg ctggggctga cagtgagcgc gtgcagtatt
    18181 atcgggctct cacgttcata ctgactagtt gtgccgaagt gttacggaac gagaaaattg
    18241 aaaaactgga agaccgtctc caggcactag aggctgccaa ggaagaggcc gaggagggga
    18301 cgaatgactg aaatggagcg gcgattgaag aaaatcgaaa cagcaggcct taatgctagc
    18361 gccgcggcgg cgctgtacca aaaatttaga aacgcaaaag acccaaagcg gcgggaaaga
    18421 ttgtataatg cgtatttagc cgccgatccg gcgagaaagt gtgaaaagtc tgccgagatc
    18481 atcgaaaaga gagtgcggat gtggcaaagc agagacagga gtgtcgatga aaagttaatc
    18541 gcaaattcag attggcggat cattggggat ggcaaagagg ggaagtgatt cgaccgccta
    18601 ctattggcgc catttcaaaa aaacggccta tagacggctt tcgcttggca gggggtgttt
    18661 ttttatacgg cctagcctta caaaaccgcg ccatggcctt ctatcagcaa cagcgggcaa
    18721 tggagagcac aaggcagtag ctacactctg cttgacatca gcttctgagg gtggtgtaac
    18781 tatgctggta cccccgaggg aattaccatg accctgctgg gagaataaga tgacccccga
    18841 cataaatggt caaaaaggag cattcctcat ggatgccaaa aaagaacagg aaagagcaga
    18901 actgcatcgc accatctgga acatcgcaaa ccagctccgt ggtagtgtgg acggctggga
    18961 tttcaagcaa tatgtgctag ggatgctgtt ttaccgctac atatccgaaa atctgactag
    19021 ttacatcaac cggggagagc gcgaagcagg aaatcctgac ttcgactatg caaaacttac
    19081 cgatgaggaa gcagagcccg ctcggatgga cttggttcaa accaaaggct tctttattct
    19141 tccaagccag ctctttgaga atgtccgcca gcgcgctgca tccgatgaaa acctgaacga
    19201 aaccctggaa aatattttcc gaagcattga ggcttccacc attggcggtc caagcgaggc
    19261 taactttaaa ggtttgttcg acgatatcga tgttaacagc aacaaactcg gccccactgt
    19321 tgccaagcgc aacgaaagac tggtcaaact tctggatgaa attgggaaca tgaagcttgg
    19381 tgactaccag gataacacca tcgatgcctt tggtgatgcc tacgaattcc tgatgagcat
    19441 gtatgcctca aatgcgggca aatctggtgg agaattcttt acgccccagg aagtttcgga
    19501 gctgttgacg cagattgcat tggcaggcaa gaccgaagtc aacaaggtat acgacccagc
    19561 ttgcggttca ggctctttgt tgctcaaggc agcaaagatt ctgggcaagg agaatgtgcg
    19621 gcaaggcttc tttgggcaag aaatcaatct gaccacctac aacctctgcc gtatcaacat
    19681 gtttctgcat gacatcgact atgacaagtt tgatattgcc catggcgata cgctgaccga
    19741 tcccaaacat tgggatgacg agccctttga ggcaatcgtg tccaatccgc cgtattccat
    19801 ccgatgggaa ggagacgcca atcctctgct tatcaatgac ccaagatttt ctcctgctgg
    19861 cgtgctagct ccaaaatcca aggcagatct ggcgtttatc atgcactcgc tagcatggct
    19921 cgcaaccacc ggcactgccg ccatcgtctg ttttccgggt gttctgtacc gaggcggggc
    19981 ggagcagaaa atccgcaaat atctgatcga taacaattat gtggattgca tcatccaatt
    20041 gccggacaat ctctttttcg gcaccagcat cgctacctgc atcatggtgc tcaagaagtc
    20101 caaaaccgat aacagtacgc tgttcatcga tgcatcaaag gagtgcgtca agattaccaa
    20161 taacaacaag ctgacgcagg aaaatatcga gacaatactt tcagcgtata ctgcccgggg
    20221 gaataaggat cactttgcga ggcttgttcc caatgccgag attgcggccc aggattacaa
    20281 tctttcggtt tccacttata tcgatcagaa agatacacgg gaagtgatcg atatcaagaa
    20341 gcttaataag gagattgaga agattgtcgc gcgggagcag gaactccgcg acgagattgc
    20401 caagattatt gctgaaatag agggtcaggc atgagcaggc tggatgagct gattgccgaa
    20461 ctttgcccgg atggggtgga atatagacta ttggctgaat tagaggatgc gaagttaata
    20521 aaattaggtc gaggcaatgt aatatcaaaa aaagaaatta gggaaatccc tggtaattat
    20581 cccgtatatt cttcgtctgc tgttggaaat ggtgaaattg gaagttatgg aaagtatatg
    20641 tttaacgatg aaagaataac atggtcaatt gatggtggcg ggagattttt ttaccgtcca
    20701 gctcaccgat attcaattac taatgtaagc gggtttatca aaattctatc aaaagaaaga
    20761 ataaatacta aatatctatt ttatgctctt attaatgaat ggagaaaaaa aatattcaac
    20821 tatacgcata aagctcatcc ttcaattatt agaaatgagt ataaaatccc catcccaccc
    20881 ctccctattc aacaggaaat agtcagaatc ctagacacct tcacggagct tgaggcggag
    20941 cttgaggcgg agcttgaggc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21061 ngcgctctgc aatgacctga ccagcggcct gcctgcggaa atcgaagccc gcaggaaaca
    21121 gtacgagtac taccgcgaca agctcctgac cttccgggag gccaaggcat gagccagtac
    21181 aacatcgtgg tggcaaccga tgaaagcacg gtggtttcag aatatgtacc ggagtattct
    21241 gccggaaacg cttaccagag cgaggcagag ctggagcaag ctttcatcaa gcagcttcaa
    21301 gcccagggct atgcctacct gcccattcat cacgaagaag agctcatcac caatctgcgc
    21361 catcagctgg aactgctcaa tagctatacc tttaccgaaa acgaatggaa caggtttttc
    21421 accaacaacc ttgccagtaa gaacgagggt atcatcgaaa agacccgcaa aatccagacc
    21481 gaccatgtcc agattctcca gcgcgatgac ggtaccacca agaacatcta cctcctcgac
    21541 aaaaagaaca ttcacaataa ccacctccag gtcatcaacc agtttgagga aacgcaaggg
    21601 cagcataaga cgcgctacga tgtcaccatt ctcgtgaatg gctttccgct ggtacatgtg
    21661 gagctgaagc gacggggcgt cgccatcagg gaagccttca accagatcaa gcgctaccag
    21721 cgtgaaagct tctgggcggc aagcggcctg tttgaatatg tgcagatctt cgtcatctcc
    21781 aatggcaccc ataccaagta ctattcaaac accacccgct cctatcacat aaaggaaacc
    21841 agcgaagcgg aacgcagcca gagcaagaaa accagtaaca gcttcgagtt cacctgctat
    21901 tgggcagacg ccaataacaa gcccattgcc gatctgatgg acttcacaaa aacctttttc
    21961 gccaagcata ccctgctcaa tgtgctcacc aaatactgcg tgttcaccac ggaagacctc
    22021 ctcctcgtga tgcgccccta ccaaattgcg gcaaccgagc gcattctttc caaaatcgag
    22081 attgccagca attacaagaa aaccggaact atcgaggcag gcggctatat ctggcatacc
    22141 accggcagcg gcaagaccct gacctccttc aaaactgccc agcttgcctc gatgctgccg
    22201 tatatcgaca aggtgctctt tgtggtagat cgcaaagacc tcgactacca gaccatgaag
    22261 gagtacgacc gcttccagaa aggcgcagcc aacagtaaca cctcgacccg cattcttcag
    22321 cggcagctcg aggacccttc ggcgaaaatc atcatcacaa ccatccagaa actggaccga
    22381 ttcatctcac tcaatcgaac acacgatgtc tacaaaaaac acgtagtcat catctttgac
    22441 gaatgccacc gctcgcaatt cggtgacatg caccgtaaaa tcataaaatc attcaagaat
    22501 tatcacctgt ttgggtttac cggcacgccg atttttgctg tcaatgccag caacaacggc
    22561 aacccgctca tgaaaaccac ggagcaggtg ttcggcgaca aactccatac ctatacgatt
    22621 gtggatgcaa tcaacgacgg caatgtcctg cccttccgta tcgactacat caacacagtc
    22681 aagatgaagg acagggtcga ggacaaaagc gtttccgcca tcgacaccga aaaggcgctg
    22741 gaagctcccg aacgcatccg cgaaattgtc tgctacatcc tcgatcattt tgaccagaaa
    22801 acaaaacgga acagcttcta taccctgaaa ggtcagcgtg tcgcaggctt caactccatt
    22861 ctggcggtga gttcgattcc catggccatg aagtactaca cggagctcaa acgccagctt
    22921 gctgagcgtg gccgtgaact catggtggcc accatcttca gctacagcgc aaacgaagcg
    22981 gaccccgaag atgtgttccc cgatgagaac ttcgataccg acagcctgcc acccaccgcc
    23041 cgcgaattcc tcgggtcggc catcgcagac tataacgcgg ccttcaatgt caacttcgat
    23101 acctcagcag ataagttcga gaattattat aaagatctgt ccctcagagt aaaaaagcgc
    23161 gaagtagacc tcctggtcgt cgtcaacatg ttcctaaccg gctttgacgc aaccacactc
    23221 aacaccctct gggtggataa aaaccttcgc ctgcatggcc tgttgcaggc cttttcgcgc
    23281 accaaccgga ttctcaattc cgtaaagact tatggcaata ttgtctgctt caggaatctg
    23341 caaaaagaaa ctgatgaggc gattgcgctg ttcggcgaca aggatgccgg cagtatcgtg
    23401 ctcttgaaag actatgacag ctattaccat ggctacaacg atgaacacgg caagcatcag
    23461 ccaggctata tcgagctgat cgaggaactg cagaccaagt ttccccttgg ccaagccatc
    23521 acaggagaag ccaggcagaa agaattcatc aaactgtatg gcgcaattct gcggctcaag
    23581 aatatcctga cggcttttga caattttgaa ggcaacgaaa ttctggctga gcgagacttc
    23641 caggactacc tgagc
//
LOCUS       DAOSOB010000028        22924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_2268_length_22924_cov_61.174603, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000028 DAOSOB010000000
VERSION     DAOSOB010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 22924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22924
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2268_length_22924_cov_61.174603"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..664)
                     /gene="tsaB"
                     /locus_tag="PLS88_04475"
     CDS             complement(2..664)
                     /gene="tsaB"
                     /locus_tag="PLS88_04475"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOU06633.1"
                     /translation="MTVLSIDTSVPTLRVGIFKDKLLAISVLKPPVTHAEAILPAVEQ
                     ALQEAGITIKNVELLAVAGGPGSFTGLRIGIATAKGLSLGLGIPIVLVPTLDIYGWAW
                     KELEGMVVPVIDARKHRVYCANYFRGQRIGNLMDIELMQLIQKINGEDNVHFVGPDAE
                     LAETICLEREGWILHKPDPEKEIESLSLLGIKTYIEKGGASDTAGPIYLREPEIGAPI
                     AR"
     gene            complement(645..1175)
                     /gene="tsaE"
                     /locus_tag="PLS88_04480"
     CDS             complement(645..1175)
                     /gene="tsaE"
                     /locus_tag="PLS88_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOU06634.1"
                     /translation="MSLIILSSPNVRRTIAIGRAIGRAAPSGSIIAFRGDLGAGKTTL
                     AKGVAQGLGITEPIISPTFTIIAEYQGRLHLVHIDAYRLLDEDEFVQTGGEELLGLPG
                     SLSLIEWSERIAGILPKETQYITMVVEEDGSRIIMIEGDWIENIDWRRFVIPRFLDLE
                     NASALIKRFQDDCTFD"
     gene            1366..2280
                     /locus_tag="PLS88_04485"
     CDS             1366..2280
                     /locus_tag="PLS88_04485"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU06635.1"
                     /translation="MERFRASDGKELAYLQFNPKNEAKAVVIVGHGMNDHKERFIHLA
                     EALTPIGTSVWIPDLRGHGDTDSDTNRGYLADKDGFERVVQDLVEMGDYASSCMGGLP
                     LYYFGHSFGALIGMALIATYGKYLHGAVLSAPPEPQKPFMDKVGGLMISIGGALKGFH
                     APAKLPNRMTFGQYAKTVIDAKTRFDWLSRDSAIVDAYISDPKCNFICTYGFYRDLLH
                     GVRKVYSDGFLESIPLSLPLLLICGSADPVIGMKEGYERLVQRFKLLGMIDFESKCYE
                     GGRHESLNEINKQEVLNDIINWFSRHIR"
     gene            2326..3510
                     /gene="pcnB"
                     /locus_tag="PLS88_04490"
     CDS             2326..3510
                     /gene="pcnB"
                     /locus_tag="PLS88_04490"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOU06636.1"
                     /translation="MRYFRSSNGNIQSKARIYSQEDHCIDISCIDREALAIVNRLVSH
                     GYSAYIVGGAVRDLLLGRRPKDFDIVTDALPNRIRKIFSNSRIIGRRFKLVHVYSNRK
                     IYEVSTFRSLSNGTVGNEYGTISEDVRRRDFTVNALYFDPLASEILDFVGGVEDIHSG
                     KLKAIIPLNRIFSEDPVRMVRGIKYASTNQFVITLPLRLAIRRDAPLLDSVSPSRLAE
                     EFYKIIATGKSVEIIQALDRYGLLQYLLPEVHASIHGDKAFKDAFLRDLASLDLLAQA
                     PESSLLNSVTAAEIIQADKRLISPFLAWFIRQRIKDFAALRDIPKEPATFRHEVFSDI
                     RVFLSPLSLPRVALEEAMEQVLQEEDLLPAPPPKRRKHGQRKKNIKPRTESMHLEVSD
                     AN"
     gene            3554..4480
                     /locus_tag="PLS88_04495"
     CDS             3554..4480
                     /locus_tag="PLS88_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06637.1"
                     /translation="MKLARYIAKRIVRLVLTLFIISTIIFFVVRVIPGDPALIIGGIE
                     SSESDIQAIRTRLGTDRPLWVQYGEFLWNTLHLDFGRSMISDEPVLSLIIQRFPLTLT
                     LAVLGLLIGILIAIPIGVLSAVHPWSSWHYIGLVISQLGMAIPSFWLGILLLLFFAIK
                     FPIFPLFGAESFSGLVLPAISLGLARAAIILRHTRASMTEELSKEYIITARAKGLPEH
                     IIRYKHALKNALLPIITIAGIEFGYMLGGAILIEQVFSLPGLGRLFLYGIYQRDFPLV
                     QGGVQFIAIVFSLVNFAADVLYAIVNPKIRLE"
     gene            4481..5470
                     /locus_tag="PLS88_04500"
     CDS             4481..5470
                     /locus_tag="PLS88_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06638.1"
                     /translation="METTEPLITVENLRVVFSRERKRAFALRGIDIDLFKNEIHGLVG
                     ESGSGKTVTAMSIMGLLPKPSSKILSGSIRFEGNELLRLSEEKLRAYRGRRIGMVFQE
                     PSKFLNPAFTIGEQIRETLIFHLGLGSREAKAIVHELIRQVGLGEDDRVFKAYPHELS
                     SGMKQRAMIAMAISCKPEILIADEPTTSLDVTLQKQILELLKSLNNAHGMGVLLISHD
                     LAVIEQIADYISVIYAGKIVENAVTEKLFKHPLHPYTRLLLNSIPRPTQRGKRLATIS
                     GQVLEPEDEPPGCVFAPRCPIAKSLCFERSPELLEHEKGHVGACHFAGEPWNL"
     gene            5458..6333
                     /locus_tag="PLS88_04505"
     CDS             5458..6333
                     /locus_tag="PLS88_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU06639.1"
                     /translation="MESLIQCHDIIKIHRGRGLDGIKKGYRAVDKVSLDIEKGSNFGL
                     VGESGCGKTTLAKAILYLDPPTSGEVIFDGVILGSLKTRELRAFRRRMQIVFQDPHSS
                     LDPRMTIIDSLSEGLINLGIERIKREKKINRLLDLVGISPSLGTRFPHEFSTGQRQRI
                     VIARALTMDPEFLILDEPVSNLDVSIQAQVVNLLLDLKKELGLTYLFISHDINLVGYM
                     CDTIAVMYKGRIVEQAPAEKLLKYPQDDYTRMLLSSIPGGGNIESPCVLAEVNALSDV
                     SASLGLRPDHEEGIK"
     gene            6330..7826
                     /locus_tag="PLS88_04510"
     CDS             6330..7826
                     /locus_tag="PLS88_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU06640.1"
                     /translation="MKRMLRSVIILGVFVIALSSIVSAQGVDSLVFGLSGNPDTLDPQ
                     KTAGTLTFQVVKSIYDTLVEPDQSGKIVPALAESWSVSSDGLSWTFNLRRDVIFHNGQ
                     SFSSKDVAATLNRIMDEKTASPKRSDYSAIREIRTPDAYTVILMLSQPYAPLLATLAS
                     GWSAILPSGLIASGHNFAAEPVGTGPFRFEKWIRDSKITMVRNDRYWIKGVPKLERIE
                     LRIIPERAVQVQGLAIGSIDALEFIDPDDLPLLQANPKVTIKKELTSLIMVMAMNCSR
                     EPLNDLRVRQAVNYAIDKQLVLDVAYGGGKIGSTFLDTGNVYYTDFSYLYPYNPEKAK
                     QLLKEAGAGNKELTITVPQNYPLHVKAAELIQQMLEKVGMKVKIRLVDWSSWLSIVYS
                     EGNYDFTVIGHTGKLDPDGTLAGYGAGRYVKWNNASAASKINEAAKIADFVTRKRLYT
                     EALEIMAKEVPFVYLGTPYRYVGTRSNVVDFRMTYNLDTYDFRWTELR"
     gene            7830..8657
                     /locus_tag="PLS88_04515"
     CDS             7830..8657
                     /locus_tag="PLS88_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06641.1"
                     /translation="MVRSSSRRLLFSLSSLYLLLILAAAIAAPHIAPYSPTEQQLERR
                     FHAPDSHNILGTDELGRDILTRIIYGSRPALVVGLVTVSIALLLGGFFGLLAGMGHNA
                     LDSSIMLLMDSMLSFPTILLAITVVSFMGYGLVQVMLALGIISSPVFARLIRAETLSI
                     KTEGYIEAARALGTPTVKIVFKHIVPNIMSKVIVQCSLTFAQAIVVESSLSFLGVGIQ
                     PPDASWGLMLKDARNYLIQAPWMAIYPGLCLALTVLSFNIIGDSLSEKFNPRLSDRL"
     gene            8800..10563
                     /locus_tag="PLS88_04520"
     CDS             8800..10563
                     /locus_tag="PLS88_04520"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOU06642.1"
                     /translation="MSVVRTTPVPPDVDIAQSADIKPISEIASRYNLGEKYLEHYGTD
                     KAKIRLEFLSDSQKQPRRGKYIDVTAITPTPLGEGKTTTTIGLAQGLGYLGKKSIATI
                     RQPSMGPTFGIKGGAAGGGYSQIVPMTDFNLHLTGDTHAVSAAHNLCAAAIDARIYHE
                     SRWSDTYFEKLGLKKLNIDPYSVLWRRVVDMNDRTLRSIIIGLGGLENGPLRETGFDI
                     SVASEVMAVLALATSLEDLRKRIGKIVVALDKSGKAVTTEDIGVAGAMTILLKDALKP
                     NALQTLEEQLAFVHTGPFANIAHGNSSITADLIASEIADFVVTESGFGSDMGFEKLMD
                     IKCRASGLMPDAVVLVATVRALKMHGGGPKVTPGKPLSSAYTQENLELLQAGLTNLVA
                     HIEIIGKYGLPVVVSINAFPSDTEAEHALIKEAALKAGAFDAVISRGWALGGEGCAEL
                     ASAIDKASSQPHKANLLYPLEISIKDKIEIIAHQVYGAGTVVYTPEADEAIEKYTDLG
                     YGNFPICMAKTQYSLSHDPAVKGVPRGFDFPVREVRLSAGAGFIVPITGEISTMPGLS
                     SKPVFIGMDIDTKTGRITGLS"
     gene            10610..11296
                     /locus_tag="PLS88_04525"
     CDS             10610..11296
                     /locus_tag="PLS88_04525"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOU06643.1"
                     /translation="MNPEILSLLSDEELKILAEKMNIYFPEGLERIFLIEEIIDAYED
                     DTSDKTFSHDAPDHLEEKKLAGTGFIPGRLEDISIPDYYNETYINAIVRDPLWIFAFW
                     DIAESTQQKYIGEAETSRFFLRITEATGFEPSFSYDIAVNFDDRKWYINVPYAKRSYR
                     IDLCIAKCHKARVLARSNIVRMPSQYLDISSKLPQVTRSLLVLSGSEELHLIEPKEEN
                     SLRIMTMEGE"
     gene            11296..12897
                     /locus_tag="PLS88_04530"
     CDS             11296..12897
                     /locus_tag="PLS88_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOU06644.1"
                     /translation="MANNSPSIALVLNAHLPFVRKPQYPQFYEERWLFEVISETYLPL
                     LRMFRKLELENIPFKVSMVFSPTLLAMLSDSLLCERYIAYLDNQIELAQKERRRLAGD
                     PVFEPLADLYYTMYSRSREEFEDLYGRNIIRAFDYFSKKGYIELMTTAATHAFLPTYS
                     DIPEAIDAQIESAIIAHRMEFGKNPSGFWLPQLGWYQGLEKHLRAYNIRYTIVTTKGA
                     LLGSPPPFYGSFSPVMTTSHIAAFIRDAGATKAVWSETEGYPAHSVYRDFYRDIGYDL
                     DPRYLGPHLYGVERGYTGFKYWAVTGKTDNKRPYEPSAASAQAVLHAHAYLSDRKVQA
                     RAASYWMKERSPIIVCPYDAELFGHWWFEGIQFLEAFFRAMSRKDEENPLRLVTLSEY
                     LTENPTCPQSEPEFSSWAEGGYAEAWLDGRNDWVLRHTRKAAERMRELTIRFPNESGL
                     RERILNQAAREVLLAMSSDWALLLRSGKSADFAARQIRESIYNFNHIYEMLSAHTVET
                     EWLTSLEKRNNIFPYMNYHVFAPKK"
     gene            12914..14140
                     /locus_tag="PLS88_04535"
     CDS             12914..14140
                     /locus_tag="PLS88_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOU06645.1"
                     /translation="MNIVIIGAQWGDEGKGKIVDFLAADAQIVVRFSGGANAGHTIVL
                     GGERFALHLVPSGILYHDKTVILGSGMVIDPEALFRELGDLENKGIDWKGRVFISDRA
                     HLVLPRYRDMDKEADSRRAKPIGTTGRGIGIAYALKASRDGIRIADIDDEERLVNLGS
                     EDREWLFQWKDRLLPLKIDLAAYLRSHRNTWRLFEGAQGALLDIDTGTYPFVSSGMSC
                     AAGAAAQGGIGPRAIDRVLGVFKAYSTRVGNGPFPTEFADDSPGSVSQYIRKVGNEYG
                     TTTGRPRRCGYLDLVALRYACAVNSIDRLVLTHLDVYDALEEFEACIGYHIGGKLTED
                     FPASIGDLEGARPVLRTFKGWKRPIGNCRTYEELPLSAREYIEFIEEFTETPISIISV
                     GSDRNQTIVRESPWIK"
     gene            14128..15699
                     /gene="guaA"
                     /locus_tag="PLS88_04540"
     CDS             14128..15699
                     /gene="guaA"
                     /locus_tag="PLS88_04540"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOU06646.1"
                     /translation="MDKIIILDFGSQYTQLIGRRIRELGVYSEILPGEKALDSATAKD
                     CVGFIISGSPFSAMEKEAPSLHSSIMESGLPILAICYGIQWLTLQSGGIVERSKTHEY
                     GPMAVSFLPIVRKENLWSLFSEGLPERFISWMSHGDSIVRPGDGWEVIGLSESGVPAI
                     LAHKFKPWFGLQFHPEVSHCEHGMDILSHFVFGICGARAAWSIEAYLDEEAEAVRSKV
                     KSEPVLLLISGGVDSTVTGAFLLRCLNPEQVYLLYIDTGLMRKAENKEVMLNLNRLGA
                     LHLEAIDAESRFLEALKGISDPEQKRQIIGDMFIQVQEEAVQSLGIPNAFLAQGTLYT
                     DLIESGKGVGNKAQVIKSHHNVRSPLIEKKRAEGRVLEPLDRLYKDEVRALGRALGLP
                     REIVERHPFPGPGLGVRILGEVTKEKCEILREADSIYIEELRRRGFYDQIWQAFCVLL
                     PVRSVGVMGDERAYRYVLALRAVKSEDGMTAQPFAFDTKDLFEISACITNRVPQIGRV
                     VYDISSKPPATIEWE"
     gene            complement(15897..16901)
                     /locus_tag="PLS88_04545"
     CDS             complement(15897..16901)
                     /locus_tag="PLS88_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOU06647.1"
                     /translation="MQNSTQTIGLPYRATAFWLSLAHESVEGCKRNEQGVPLDPILAQ
                     AIPSAAESRVSPWDRCDPLGEENHSISDRVIRHYRSRVLVRATGQCFLFCRHCYRCSL
                     LPSEFEFIDEANIDKLIFLLKSHSEIREVLISGGDPLTAPDEKLKGLLYAIRSVKRPI
                     LIRLCTRAPVVLPQRISDGLISLLLEMYPLQIVLHINHPRELSSEFFEKSEALLKAGI
                     PLHSQTVLLRGINNSAETLIELFSTLALHSIHPYYLFQGDLAIGTAHFRVPLSQGLSI
                     YENLKIELSGLELPRYAVDAPGGGGKVYLPEGIVERRGSVWVLRTPDGSLCEYPEEPL
                     "
     gene            17163..17639
                     /locus_tag="PLS88_04550"
     CDS             17163..17639
                     /locus_tag="PLS88_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HOU06648.1"
                     /translation="MKERAERLKVIKAIIKENRVDSQEKLLQLLEDHGYSVTQATLSR
                     DLKYLKVGKLSDGHSGYYYSIPSEEERRESERHYIQDFLRGYVSIDWNQSLVVVRTFS
                     GHSDSVALAIDNMGFDEVIGTISGRDNVVFIALKKGVSGDDFIRSLKARVPEFEEE"
     gene            17792..20380
                     /gene="clpB"
                     /locus_tag="PLS88_04555"
     CDS             17792..20380
                     /gene="clpB"
                     /locus_tag="PLS88_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOU06649.1"
                     /translation="MNYEQFTLKAQEALRDAASIAQKNDHSSIEPEHLLLALLDQEGG
                     VVSSILERLGVDRAKLEQRLSDLLKTLPKAYGEATQVYFSPAMQKVIAKAESESESMK
                     DEFVASEHLFLALLIVESKAASLLRSIGVTHEGVLAVLKSIRGSQTASDENAEEKYQV
                     LEKYTRDLTTLARAGKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIISGDVPDSLKDKKVLALDLGAIVAGSKFRGEFEERLKAVITEITKSEGKIILF
                     IDELHTLVGAGAAEGAMDASNLLKPALARGELRAIGATTLDEYRKHIEKDAALERRFQ
                     PIYCAPPSVEDTIAILRGLKERYEVHHGVRIRDDALIAAAVLSDRYITSRFLPDKAID
                     LVDEAASRLKMEIESQPTELDQIQRRLIQLRMEADALKKETDPASIERRNKLEKEIAE
                     LSETRDEMTARWQQEKRLIEEIRQLKQQIEEYKIQVDQLVREGQLAKASEIKYGKLVE
                     AQQKLEELSKKVQEEEGKPRLLREEVSDEDIAKVVSLWTGIPVSKMLSSEIQKYVELE
                     SQLGKRVIGQERALQAVADAIRRNRAGLSDPRRPLGSFLFVGPTGVGKTELAKALAEF
                     LFNDERALTRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLFDEI
                     EKAHPEVFNVLLQLLDDGRLTDGQGRVVDFRNVIIIMTSNIGSEFILTMKSMDEIRDR
                     IDGLLKTYFKPEFLNRIDEIVVFDRLNESMIRKIAQLRLSELGERLTQRNIKLEVEDK
                     AIDYIAKEGYDEQFGARPLKRAIQNLLENPIAKKILAGEIMEGDTIHVSLSGSELAIG
                     TKHSSE"
     gene            complement(20401..21261)
                     /locus_tag="PLS88_04560"
     CDS             complement(20401..21261)
                     /locus_tag="PLS88_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06650.1"
                     /translation="MLLYPDLSQAVAREFPLENVANPKVAQQLLEKKIEKKTTDNQSL
                     NIFITSPSVAIAFQEFASDDRFAVINLLTPANGFCSVEWDNAWAYQQLGLLTGYRIAA
                     LRKETAKDASAALLFARGIGRSDKELEVFNQAFREGYLRATVDNKQKQSLDQVGMIPE
                     NALEIFDVDNMNLPGDRLEQTLSVLRQIEEKKLTILIFACTSRLALERAREIQGIDIM
                     ADLRGLGKDISHKRLFAALGENSSNLIRVLRNTVKEVQNGNTEPHSEYIRPDLILSKE
                     ARRIQQKLKP"
     gene            21494..22492
                     /locus_tag="PLS88_04565"
     CDS             21494..22492
                     /locus_tag="PLS88_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOU06651.1"
                     /translation="MISYKELGLVNSVELFRKAMEGGYAIPAYNFNNMEQMQAIIQAC
                     VETRSPVILQVSSGARKYANATLLRSMAKGAVEYAKELGYPIPIVLHLDHGDSFELCK
                     DCIENGFSSVMIDGSHLPYDENVALTKRVVEFAHAQKDYVSVEGELGVLAGIEDEVSA
                     ERSHYTRPEEVEDFVKKTGVDSLAISIGTSHGRAKFKPEQCTRTPDGVLIPPPLRFDI
                     LEEVEKRIPGFPIVLHGSSSVPVEYVRMIEQYGGKLPDSVGIPEDQLRKAARSAVCKI
                     NIDSDGRLAMTAIVRKVLFEHPEEFDPRKYLGPARDELKKLYMHKNIEVLGSANRA"
     gene            22668..>22924
                     /gene="infC"
                     /locus_tag="PLS88_04570"
     CDS             22668..>22924
                     /gene="infC"
                     /locus_tag="PLS88_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOU06652.1"
                     /translation="MAEKDLRINEQIRVREVRLIDENGEQRGIVPTLEALRIAHEAGL
                     DLVEVAPQSVPPVCRLLNYGKYKFEQEKKVKDAKKRAKVTEL"
BASE COUNT         6354 a         4807 c         5512 g         6251 t
ORIGIN      
        1 atcagcgcgc tatcggagcg cctatttctg gttcacggag atatatgggt cctgcggtat
       61 cgctagcacc gcctttttct atatatgtct taataccaag aagagagaga gattctattt
      121 ccttttccgg atctggctta tgtaatatcc atccttctct ttctaagcag atagtctcgg
      181 ctagttcagc atcgggacct acaaaatgaa cattatcttc gccattaatc ttttggatga
      241 gctgcatgag ctcgatatcc atcaaattac ctattctttg gccgcggaag tagttagcac
      301 aatagactct atgctttcgt gcatcgataa ctggcacaac catgccttcg agttctttcc
      361 aagcccatcc atatatatct agagtaggga cgagcactat aggaattccg agtcctagag
      421 atagtccttt tgccgttgcg attcctatcc gtagacctgt aaatgagcct ggacctcctg
      481 caactgcgag taactccaca ttttttatcg taattccagc ctcttgaagt gcttgttcca
      541 ctgcaggaag aatggcttca gcatgagtga caggtggctt aagtacagat atcgcgagca
      601 gtttatcttt gaagatacct actctaagtg taggaacact tgtatcaatc gaaagtacag
      661 tcatcttgaa acctctttat caaggctgaa gcattctcca gatctaaaaa gcgcggaatc
      721 acaaaacggc gccaatcgat attctcgatc caatcacctt cgatcataat aattctgcta
      781 ccgtcttctt cgacaaccat ggtgatgtat tgggtttcct ttggcaatat tcctgcgatc
      841 ctctcgctcc attctataag actcaacgag ccaggcaaac caagtagctc ttcgccgccg
      901 gtctgcacga attcgtcctc gtccaagaga cgatatgcat ctatgtgaac aagatgaagg
      961 cggccttgat attcggctat tattgtgaat gtaggcgaga ttataggctc cgttattcct
     1021 aaaccttgag caactccttt agcgagagtt gtcttcccag cgccaagatc accgcggaat
     1081 gcaatgatag acccgcttgg cgctgcacgc cctattgctc tgcctattgc gattgttcta
     1141 cgtacattag ggctagatag aataatcaga gacaagcctc cctcgatatt tcctaatttt
     1201 ctatagtatg cgccaaaggc ggatttttct agaccttgat tgaaagaggc gtataatgga
     1261 aagtcaaggc tggacttttg gctcgctaag cacaataata atttaatgag aacaaaatat
     1321 gccgataatc aaacaaagat aagcatttaa tattgagagg ctcagatgga acgatttaga
     1381 gcaagcgatg gcaaagaact agcttatctg caattcaacc ctaaaaatga agcaaaagcc
     1441 gtggttatcg ttggtcatgg catgaatgat cacaaagagc gttttataca tcttgctgaa
     1501 gcacttacgc cgattggtac ttccgtctgg attcctgatc ttagaggtca tggcgatacc
     1561 gattctgata ccaatagagg ctatctagct gacaaggatg ggttcgaacg cgttgtccag
     1621 gatttggttg aaatgggaga ctacgcttcg agctgtatgg ggggattgcc actttattat
     1681 tttgggcatt catttggagc ccttattggc atggctttaa ttgctactta cgggaaatat
     1741 cttcatggag cagtgctttc cgctccaccc gagccgcaaa agccttttat ggataaggta
     1801 ggtggtttga tgatttctat tggcggagca ttaaagggct ttcatgctcc tgcaaagctt
     1861 ccaaatcgga tgacctttgg ccaatatgca aaaactgtaa tagatgcaaa gacaagattc
     1921 gactggcttt ccagggattc ggcgatcgtc gatgcctaca tttcagaccc taaatgcaac
     1981 tttatctgca cttatggatt ctatcgcgat cttcttcatg gtgttagaaa agtatatagt
     2041 gatggctttc tagagagcat tcctctatcg cttcctctat tattaatatg tggctctgcc
     2101 gacccagtta taggcatgaa agagggatat gaaaggcttg tgcagagatt caaacttctt
     2161 ggcatgatcg attttgaatc taaatgctat gagggaggca ggcatgagtc gctcaatgaa
     2221 atcaataagc aggaagtact gaacgacatc atcaactggt tctctcgaca cattcgatga
     2281 atttttgtac tgtatgagca tgcattttgg agcagctttg tgagaatgcg gtactttcgt
     2341 agttcaaatg gtaatataca gtcaaaagct cgaatataca gtcaagaaga tcattgcatc
     2401 gatatatctt gcattgatag agaagcgcta gcaatagtaa ataggctagt ctcgcacgga
     2461 tattcagcct atatcgtagg aggagctgta cgcgatttgt tgcttggaag gcggcctaaa
     2521 gacttcgaca tagtaaccga tgccttaccc aatcgtattc gaaaaatatt tagtaattct
     2581 cgaatcattg gaaggcgctt caagctcgtc catgtttatt ctaataggaa gatttacgag
     2641 gtgagcacat ttcgctcttt gtcgaatggt actgtcggca atgaatatgg gacaatcagc
     2701 gaggatgtac gacggcgaga tttcactgtt aatgcgcttt actttgatcc gctcgcttca
     2761 gagatactgg attttgtcgg gggagtcgag gatatacatt caggtaagtt aaaggcgatt
     2821 atacctctaa ataggatttt tagcgaagat cctgttcgta tggtgcgcgg tattaagtat
     2881 gcttcaacca atcaatttgt aattacattg ccattgagat tggcgattcg gcgtgatgcg
     2941 ccgcttcttg attcagtaag cccatcaagg cttgcagaag agttctataa gataatagct
     3001 actggaaaat cagtagaaat catccaagca ttggatagat atggcctttt gcaatattta
     3061 ttgcctgagg ttcatgcttc aatacatgga gataaagcat tcaaggatgc atttttaaga
     3121 gacttggctt ctctcgatct gctggcccag gcgcctgaga gtagccttct aaacagcgta
     3181 accgccgctg aaattattca ggcggataaa aggctcatat ctccatttct agcttggttc
     3241 atccgccaga ggatcaagga tttcgctgct ctcagagaca ttccgaaaga acctgccaca
     3301 ttcaggcatg aggtattttc ggatattcgg gtatttctat cgccgctcag cttgcctcga
     3361 gtcgctttgg aggaagcaat ggaacaagtg cttcaagaag aagatttgct tcccgctcct
     3421 cctcctaaaa gaagaaaaca tggtcaacgg aagaagaata ttaagccgcg caccgagtca
     3481 atgcacctcg aagtttcaga tgcgaattag cgataaatgt cggtagatcc gcagcattac
     3541 gagggagcaa aaaatgaaat tggcgagata tattgcaaaa cgaattgtca ggctcgttct
     3601 tacattgttt ataatatcga caatcatttt tttcgttgtt cgagttattc ccggagatcc
     3661 tgcgctcata ataggaggaa tagaatctag tgaatctgat atccaagcaa taagaacaag
     3721 attgggtaca gataggcctc tctgggttca atacggagag tttctatgga acacgcttca
     3781 tttggatttt ggacgatcga tgatttccga tgagccggta ttatctttga ttattcagcg
     3841 ctttcctctt acattgacac ttgcggtctt agggctcttg ataggcattc ttattgcaat
     3901 accaatagga gtgttgtctg ctgttcatcc ctggtcatcg tggcattata tagggcttgt
     3961 gatatctcag cttggtatgg caatacctag tttttggctc gggattctgt tgctcctttt
     4021 ttttgctatt aagtttccaa tttttcctct ctttggtgca gagtcttttt cgggattggt
     4081 tctgcctgcg atttcgcttg ggcttgcgcg agcagcaatt attttgcgcc ataccagggc
     4141 ctcgatgacg gaagagcttt cgaaagaata cataataact gcaagagcaa aaggtcttcc
     4201 cgaacatata attcgctaca aacatgcact taaaaatgct cttttaccga ttatcacgat
     4261 tgctggtata gaattcggat acatgctggg tggagcgatt cttatcgaac aggtattttc
     4321 gctaccgggg cttggaaggc tttttttata tggcatttat cagagagatt ttccgcttgt
     4381 acaggggggt gtgcaattta ttgctatagt tttctcatta gtgaattttg cggctgatgt
     4441 gttgtacgcc attgtgaatc cgaaaataag gctggaataa atggaaacta cagagccgct
     4501 cataaccgta gaaaacttgc gagttgtttt ttcaagagaa agaaagagag cttttgctct
     4561 ccgtggtatc gacattgatc tttttaagaa tgaaattcac ggtttggtcg gtgaatcggg
     4621 atctggaaag accgtgacag ctatgtctat tatggggctt ttgccaaaac cttcttcgaa
     4681 aatcctatca ggaagtattc gtttcgaagg caatgaactt cttaggctct ccgaggaaaa
     4741 attgagagca taccgaggtc gcagaattgg aatggtattt caagaacctt cgaagtttct
     4801 taatcctgca tttacaattg gagagcagat tagagaaaca ttgatctttc atttggggct
     4861 agggtctcga gaggcaaagg cgatagttca tgaactaatt cggcaagtcg gtctaggaga
     4921 agatgataga gttttcaaag cctatcccca tgaactttct agtggaatga aacagagagc
     4981 catgatcgca atggcaattt cttgcaaacc tgagatttta attgccgatg aaccgactac
     5041 ttctctcgat gtaacgcttc aaaagcagat actggaactg ctaaaatcac tcaataatgc
     5101 acatgggatg ggtgtactcc tcatctctca tgatctagct gttatcgagc agattgctga
     5161 ttacatctct gtaatatatg cgggcaaaat cgtagaaaac gcggtgacag aaaagctatt
     5221 caagcatccc cttcatcctt atacaaggct ccttcttaat tcgattcccc gtcccactca
     5281 gcgaggaaaa cggctagcaa ccatctcggg gcaggtatta gaaccagagg acgaaccacc
     5341 aggatgcgta tttgcaccgc gctgccctat tgcaaaaagc ttgtgtttcg aaagaagtcc
     5401 agagctttta gagcatgaaa agggacatgt aggcgcttgt cattttgcgg gggagccatg
     5461 gaatctttga ttcaatgtca tgatattatc aaaatacatc gcggtagagg cctagatggt
     5521 ataaagaagg ggtatagggc cgtagacaag gtatctctcg atattgaaaa aggtagcaat
     5581 ttcggtcttg taggcgaatc aggttgtgga aaaactactc tagcaaaggc aattttgtat
     5641 ttagacccgc caacctcggg agaagtgatt ttcgatgggg tgatactggg gagcctaaaa
     5701 acacgagaat tgagggcttt taggaggagg atgcagattg tattccagga tccgcacagc
     5761 tcactagacc ctcgaatgac aattatcgat agtctttctg agggattgat taaccttgga
     5821 atagaacgaa tcaagcgcga aaaaaagata aatcgccttc ttgatcttgt cggcataagc
     5881 cctagcctcg gaacacgctt ccctcatgag ttttcgacag gacagcgtca gcgaattgta
     5941 attgccagag ctcttactat ggatccagaa tttcttatcc tcgatgagcc agtctccaat
     6001 ctcgatgtct ctatccaggc acaagtggta aacttactgc tcgatctaaa aaaagaacta
     6061 ggccttacct atctatttat ttcgcacgat ataaacctcg ttggatatat gtgcgataca
     6121 attgcagtga tgtacaaggg aagaattgtc gaacaggctc cagccgagaa acttctcaaa
     6181 tatccgcagg atgattatac tcgaatgtta ctatcctcta ttccaggagg aggaaatata
     6241 gagagcccgt gcgtattagc agaagtaaat gcgctatccg atgtttcagc ctcgttagga
     6301 cttagacccg accatgagga gggaatcaaa tgaaaagaat gctaagaagc gtaattatcc
     6361 taggtgtttt tgtgattgct ctctcaagca ttgtgtctgc ccagggtgtt gattcattag
     6421 tctttgggct ttctggtaat cctgatacac tagatcctca aaaaactgct ggaaccttga
     6481 cattccaagt tgtcaaaagt atctatgata cgctagtcga acccgatcaa tcgggcaaga
     6541 tcgtaccagc gctagcggag agctggtcag tatcgtcaga tggcttatcc tggacattta
     6601 acctaaggcg ggatgtcata ttccataatg gacagtcttt tagttcaaaa gatgtggcag
     6661 ctaccctgaa tcgaataatg gatgagaaaa cggcttctcc caaaagaagt gattattcag
     6721 caattagaga gattagaact ccggatgctt acactgtaat acttatgctt tctcagcctt
     6781 atgctcctct tcttgcaacg cttgcttctg gctggtcagc gatccttcct tcaggactga
     6841 ttgcaagcgg gcataacttt gctgccgagc ctgtaggaac aggtcctttt agattcgaga
     6901 aatggatacg cgactcgaag atcactatgg ttcgaaacga tagatactgg atcaagggtg
     6961 ttcctaagct tgagcggatc gaattacgga ttattcctga acgagccgtg caagtgcagg
     7021 gccttgctat cgggtccatc gatgccctcg aatttatcga tcctgacgat ttacccttgc
     7081 tacaagccaa tcccaaggtc accataaaga aagaattgac ttctcttata atggttatgg
     7141 caatgaattg ttctcgagaa cccctcaacg atctgcgtgt gcgtcaagcc gtgaactatg
     7201 caatcgataa acagcttgtg ctagatgttg cttatggagg aggtaaaatt ggttcgacat
     7261 ttctcgatac ggggaacgtt tattataccg atttttctta tctctatcca tacaatccag
     7321 aaaaagcaaa gcagcttttg aaggaagcgg gagctggcaa taaggaattg actataaccg
     7381 taccgcagaa ttatccgctt cacgtaaaag ctgctgaact cattcagcaa atgctcgaga
     7441 aggtaggtat gaaagtcaaa attcgacttg tagattggtc gagttggctt tcgatagtct
     7501 atagtgaagg caactatgat tttacagtga tagggcatac aggcaagcta gaccctgatg
     7561 gaaccctggc gggatatggg gctggtagat atgtaaaatg gaacaacgca agtgcagctt
     7621 ccaagatcaa cgaagccgct aagatagctg atttcgttac cagaaaaagg ctctacacgg
     7681 aggcactcga gatcatggct aaagaggtcc cttttgtata tcttggaacg ccttatcgat
     7741 atgtaggtac aaggtcgaat gttgtcgatt ttcgcatgac atacaatctg gatacttatg
     7801 attttagatg gacagagctg cgatagaata tggtacgtag tagttctaga aggcttctct
     7861 tttcactttc ctctctttat ttgttgctga ttcttgcagc tgcgattgcc gctcctcata
     7921 tagcgccata tagtccaacg gagcagcaac tcgagcggcg ctttcatgcg ccagacagtc
     7981 acaacatact cggaaccgat gaacttggaa gagacattct gacaagaata atctatggtt
     8041 cacggccagc cttggtagtc ggcctagtga ctgtgtctat agcacttctt ctgggcggtt
     8101 tttttggact tcttgctggc atgggtcata atgccctgga tagttcgatc atgcttctca
     8161 tggatagcat gctatctttt ccaacgatcc ttttggccat aacagttgta tcctttatgg
     8221 gatatgggct ggtacaagtt atgcttgcat taggtatcat aagtagcccc gtatttgcgc
     8281 gtcttattag agctgagact ctatcgataa agaccgaagg atacatagag gctgccagag
     8341 ctcttggaac accgacagta aaaatagtat ttaagcacat tgttcctaat ataatgagta
     8401 aagtcatcgt gcaatgctcc cttacttttg ctcaggcaat tgttgtcgag tcttctctct
     8461 cgtttctggg tgttggaatt cagcctccag atgcaagctg gggactcatg cttaaggatg
     8521 ctcgcaatta cctgatacaa gctccctgga tggcaatata tcctggacta tgccttgcat
     8581 tgacagtgct ttctttcaac ataattggag attctctttc ggagaagttc aatccacgtc
     8641 taagcgacag gttatgaaat tgatatattt gagaatgaca gcattttgga tcgaattact
     8701 atgccggcct cagttgcctt cttcgagcct ataaagtaaa atatttatgt tccaaatttt
     8761 aagcagcttt tctttaatgt atcttaaagg aggtttttca tgtctgttgt tcgaacgact
     8821 ccagttccac cagatgtaga cattgctcag tcggcagata taaagccgat atccgagatt
     8881 gcgtcacgct ataatctagg agaaaaatat ctcgagcact atggaaccga caaggcaaag
     8941 attcggctag aattcttatc agatagccag aaacagccga ggcgaggcaa gtatatcgat
     9001 gtaactgcaa ttacacctac gccccttggt gaaggaaaga caacaacgac cataggcctt
     9061 gctcaaggct tgggatatct tggcaagaaa tctattgcga ccatccgtca accttctatg
     9121 ggaccaacct ttggcataaa gggcggtgca gcgggagggg gatatagtca gattgttcca
     9181 atgacagatt tcaatctgca tctgacaggc gatacacatg ccgtgtctgc agcacataat
     9241 ctatgtgccg ctgctatcga tgcacgcata taccatgaga gcagatggtc ggatacatat
     9301 ttcgaaaagc tgggcttgaa aaaattaaat attgatccat attcggttct atggcgacgt
     9361 gtagtcgaca tgaacgacag aactctgagg agcataatca taggcttggg cggcttggag
     9421 aatggcccgc tgcgcgaaac aggcttcgac atatccgttg caagcgaggt aatggcggta
     9481 ttggccctgg ctacgagcct cgaagatcta cgcaaacgta taggaaagat tgttgtagct
     9541 ctggataagt caggtaaagc ggtgactacc gaagatattg gggtagcagg agcaatgacg
     9601 attctcttga aggatgcatt aaaacccaat gctctccaaa cgctcgaaga gcagcttgcc
     9661 tttgtccata caggaccttt tgccaatatt gcacatggca attcttccat aactgcagac
     9721 ctaattgcta gcgagattgc ggatttcgta gtaacagagt caggattcgg ttctgacatg
     9781 ggattcgaaa aactgatgga tattaaatgc agagcttcgg gtcttatgcc agatgcagtg
     9841 gttcttgtgg caacggtaag agcactcaag atgcatggtg gtgggccgaa agtgacgcct
     9901 ggcaaaccgc tttccagcgc ttatactcaa gagaacttgg agcttttgca ggcaggattg
     9961 acaaatctcg ttgctcacat tgagatcatt ggaaaatatg gacttccggt ggttgtctct
    10021 atcaatgcat tcccaagcga tacagaagcc gaacatgcgc ttatcaagga agcagctctc
    10081 aaagcaggcg ctttcgacgc agttataagc aggggctggg cccttggagg agaaggctgt
    10141 gctgagctag cctcggcgat agataaggct tcatctcagc cacataaagc taatttatta
    10201 tatcctctgg aaatatccat aaaagataag atagagataa tcgctcatca ggtttatgga
    10261 gctggaacag tggtatacac accagaggcc gatgaagcaa ttgaaaagta taccgatctt
    10321 ggctatggaa attttccaat atgtatggca aagacacagt attccctatc gcacgatcct
    10381 gccgtgaagg gggtaccaag aggtttcgat ttcccagtac gagaagttcg gctttcggcc
    10441 ggagcgggtt tcattgtccc gattacaggc gagataagta caatgcctgg actgtcatcg
    10501 aaaccggtgt tcattggtat ggatatcgat accaagactg ggagaatcac tggattgtct
    10561 tgacccatgc atattgacaa ggacgggctc attctttcac tatactcttg tgaatccgga
    10621 aatactgtca cttctttcgg acgaagaact caagatttta gctgagaaga tgaatatcta
    10681 ttttccggaa ggactagaaa gaatcttttt gatcgaagaa atcatcgatg cttatgaaga
    10741 tgatacttcg gataagacat tttctcacga tgctcccgat catctagagg aaaaaaaact
    10801 cgcgggcacg ggttttattc ctggcagact agaggatatt tctattccgg attactacaa
    10861 tgaaacatat atcaatgcaa tcgtaagaga tccattgtgg attttcgcat tctgggatat
    10921 tgcagaatct actcaacaaa aatacatcgg agaagccgag acatctcggt ttttcttgcg
    10981 gataactgaa gccaccggat tcgagccaag tttttcctac gacattgccg tgaacttcga
    11041 tgatcgtaaa tggtatataa acgttcccta tgcaaagcgt tcttacagga tagacctatg
    11101 catcgcaaaa tgccataaag ccagagtgct agctcgttcc aatattgtac gaatgccatc
    11161 acaatacctc gatatatctt ccaaacttcc gcaggtaaca aggagccttc tagttctttc
    11221 tggttccgag gagcttcatt tgatcgagcc taaagaggag aattctctgc ggataatgac
    11281 tatggaagga gaataatggc aaataattcc ccttcgatag ctcttgttct caatgcccat
    11341 cttccttttg tgcgtaagcc tcaatatccc cagttctatg aagaaaggtg gctttttgag
    11401 gttatttcgg aaacatatct tccattattg aggatgtttc gcaaattgga gctcgaaaat
    11461 attcctttta aggtgagcat ggtcttttct ccaactctgc tagccatgct ttcagatagt
    11521 cttctttgcg aacgatacat cgcctatctt gacaaccaaa tcgagcttgc tcagaaggaa
    11581 agacgaagat tagctgggga tccagtattt gagcccctgg cagacctata ttacactatg
    11641 tatagcagga gccgcgaaga attcgaagat ctttatgggc gcaatattat tagagccttc
    11701 gattattttt ccaagaaagg ctatatagag ctcatgacca ctgctgccac gcatgctttt
    11761 cttccaactt actcagatat tcctgaagca atcgatgccc agattgagtc tgcaattatt
    11821 gcccatagaa tggaatttgg aaagaaccct tcgggattct ggctgcctca acttgggtgg
    11881 tatcaggggc ttgagaagca tctcagagcc tacaacatcc gatatacaat agtgacaact
    11941 aagggcgcat tgcttggttc gccccctcct ttctacggat cgttttctcc agttatgact
    12001 acttcacata ttgctgcttt tatacgcgat gctggggcaa cgaaggcagt ttggtctgaa
    12061 accgagggtt atcctgcgca ttcagtctat agagatttct atagagatat agggtatgac
    12121 ctcgatccta gataccttgg ccctcattta tatggtgttg agcgcgggta tactggattc
    12181 aaatattggg cagtgactgg taagacagac aacaagcgtc catatgagcc atcggcagca
    12241 tcggctcagg ctgtgctgca tgcgcatgcc tacctttcag acagaaaagt tcaagcgcgc
    12301 gctgcttcct attggatgaa agaaaggtct ccgatcatag tatgtcccta cgatgcggaa
    12361 ctctttggcc attggtggtt tgaaggcatc cagtttctag aagctttttt tcgtgctatg
    12421 agccgcaaag atgaagaaaa tccacttcgt cttgtaactc tgtctgagta tttgacagag
    12481 aaccccacct gtcctcagtc cgagccagaa ttctcttcct gggcagaagg agggtatgcc
    12541 gaggcttggt tggacggacg caacgactgg gttctacggc atactagaaa agctgctgag
    12601 cgcatgagag agttgactat tcgctttcct aatgagagtg gattgagaga acgtattctc
    12661 aatcaggcag ctcgtgaggt tctactggcg atgtcttcgg actgggctct tttattgagg
    12721 agtggcaaat cggcggattt cgctgccagg caaattcgtg aatcgattta caattttaac
    12781 cacatatatg aaatgctctc tgcccatact gtagagacag aatggctcac aagtctggaa
    12841 aagcgtaaca atatatttcc atacatgaat taccacgtat ttgcacctaa gaaatagcaa
    12901 ttaggagtct atagtgaata ttgtcataat aggagctcag tggggggacg aaggaaaagg
    12961 caagattgta gatttcttgg ccgcagatgc ccagatagtt gtgcgctttt ctggaggcgc
    13021 caatgctggt catactattg ttctaggtgg agagcgattt gcccttcatc ttgtgccctc
    13081 aggtattcta tatcatgaca aaacagtcat cctcggttct ggtatggtca tagatccgga
    13141 agcgcttttt agagagctag gtgacctaga gaataagggc atcgactgga agggcagggt
    13201 atttatttcg gacagagctc atctggtttt gcctagatat cgagatatgg ataaagaagc
    13261 cgattctcgg cgtgcaaaac caataggaac aactgggaga ggtattggta ttgcctacgc
    13321 gcttaaggca agcagagatg gcatcagaat cgctgatata gacgatgaag aaaggctagt
    13381 caatctgggt tcagaagata gggaatggct ctttcaatgg aaggatagac ttttgccttt
    13441 aaagatagat ttggctgcct atctgcgttc tcaccgcaat acctggcggc ttttcgaagg
    13501 ggctcaaggc gctttgctcg atatcgatac aggaacctac ccatttgttt cttcaggcat
    13561 gtcatgcgcc gcgggcgctg ctgctcaagg gggaatcggt ccgcgagcaa tcgatagagt
    13621 gttgggagtg ttcaaggcct attccacaag ggttggcaat ggccctttcc cgacagagtt
    13681 cgcggacgat tcccccggct cggtgtccca atatattcgg aaggtgggga atgagtatgg
    13741 tacgacaacg gggcgcccgc ggcgctgcgg ctacctagat cttgttgctc ttcgctatgc
    13801 ttgtgccgtg aactctatcg ataggctagt gctaactcac ctagatgtgt atgacgctct
    13861 tgaagaattc gaggcatgta taggatatca tataggaggc aagctgacgg aggattttcc
    13921 agcttcgata ggagacctgg agggtgctcg gccagtgctt agaacattca aaggatggaa
    13981 gcgccccatt ggaaattgca ggacctacga ggaattaccc ttgagcgctc gtgaatacat
    14041 cgaattcata gaggaattca ccgaaactcc catttcgata atctctgttg gttcggatcg
    14101 aaatcaaacc atcgttcggg aaagtccatg gataaaataa tcattctcga ttttggcagc
    14161 cagtataccc agctcatcgg ccgccgtatt agagagctag gtgtatattc agagatttta
    14221 cctggagaga aagcactgga ttcagctaca gctaaagatt gtgtaggctt tatcatttct
    14281 ggctcacctt tcagtgcaat ggaaaaagaa gccccatctc ttcattcgag cattatggaa
    14341 tcaggccttc caatacttgc tatttgctat ggtattcagt ggcttactct acaatcgggt
    14401 ggcattgtag agcgctccaa aacacacgaa tacggtccta tggctgtaag ctttttgccc
    14461 atagttagaa aagagaatct ttggtctcta ttctcggaag ggcttccaga gcgatttata
    14521 agctggatga gccatggaga tagcattgtg cgcccagggg atggatggga agtcatcggt
    14581 ttatcggaaa gcggggttcc tgccattctt gctcataaat tcaaaccatg gtttggattg
    14641 cagtttcatc ccgaagtcag ccattgcgag catggaatgg atattctatc tcatttcgtg
    14701 ttcggtatct gtggagctcg agccgcttgg tcaatagaag cctatctcga tgaggaagca
    14761 gaggctgtta ggagcaaagt aaaatctgaa ccggttttgc ttttgatctc aggaggtgtt
    14821 gattcgacag ttacaggagc ctttttgctg agatgcttaa atcctgagca agtctacctc
    14881 ttatatatag atacaggcct aatgagaaaa gcagagaata aagaggtcat gttgaatctt
    14941 aatcgccttg gagccctgca tctggaagcc attgatgcag aatctcgatt tctcgaagca
    15001 ctcaagggta tttcagaccc tgaacaaaag cggcaaataa ttggcgatat gttcatacag
    15061 gttcaagagg aagccgttca gagcttggga atccccaatg catttcttgc acaggggacg
    15121 ctctataccg atttgattga atcgggcaaa ggggttggca acaaggctca ggtaataaag
    15181 agtcatcaca atgtgcgcag tcctcttatc gaaaagaaga gagcagaagg ccgagtccta
    15241 gagcctttgg atagactcta taaagatgag gtacgggcgc ttggaagggc attgggcttg
    15301 cctagagaaa ttgtagagcg gcatcctttt cctggtccag ggctcggcgt gaggattcta
    15361 ggcgaggtga caaaagagaa atgtgaaatc ctgcgagaag cggatagtat ctatatcgaa
    15421 gaactcagaa ggcgaggttt ctatgatcag atatggcaag ctttctgtgt gcttcttcct
    15481 gttcgctcgg tgggtgtgat gggagatgaa agggcttaca ggtatgtgct agcgcttcgg
    15541 gccgtcaaga gcgaagatgg catgactgct caaccttttg cctttgatac aaaagacctt
    15601 ttcgagataa gcgcatgtat cacgaatcga gtgccgcaga ttggcagagt tgtctacgac
    15661 atctcctcga aaccgcctgc gactatcgaa tgggaataac aatttggatg agatgagcag
    15721 gattgttaga caggatgact gaatgggctg gatgaacagg ataatttatt tattagtcaa
    15781 gaaatagaga gactttgggt tgattttagt gtatgatcaa atcctgttca tcattgaaaa
    15841 tcctgaaaat cccatcgagt agtatcttgt gcatcatatc aaagcttcat gacactctat
    15901 agaggttctt ctgggtattc gcaaaggctt ccgtcgggag ttcgaagcac ccaaactgag
    15961 cctctgcgct caacaatacc ttcgggaaga tatacttttc ctcctcctcc cggtgcatcc
    16021 actgcgtagc gcggaagttc caaacccgat aattctatct ttagattctc gtagatggat
    16081 agcccttgag aaagaggtac gcggaaatgc gccgtaccaa tggccaaatc tccttgaaag
    16141 agataataag gatggatgct atgcagggca agtgtcgaaa aaagctcgat caaagtctcg
    16201 gcactgttat tgattccccg caaaaggact gtctgactat ggagtgggat accagctttg
    16261 agaagagcct ctgatttttc aaaaaattca ctgctcaatt ctcgcgggtg attgatatgg
    16321 agcacaattt gaagagggta catttctaat aagagtgaaa tgagaccatc gcttattctc
    16381 tgaggcagga ctacaggcgc tcttgtgcat agcctaataa gtattggcct ttttaccgag
    16441 cggatagcat atagcaagcc ctttagcttc tcatctggtg ctgttagagg atctccacct
    16501 gaaatcaaga cttcacggat ctccgaatgt gacttaagaa gaaatatcag cttgtcgata
    16561 ttagcttcat cgataaattc gaattccgat ggcagaagag agcacctata gcaatgtcga
    16621 cagaagagaa agcattgacc agttgcccgt actagcacgc gagatctgta atgccttata
    16681 acacggtcgg agatcgaatg attttcttct ccaagcggat cgcatctatc ccaaggagaa
    16741 acgcgcgatt ccgcggcgga tggaatagcc tgagcaagaa tggggtcgag gggtaccccc
    16801 tgttcatttc tcttgcagcc ctccactgac tcatgagcga gactaagcca gaaagctgtc
    16861 gcgcgatatg gcaagccaat tgtctgagta gaattctgca tctctgatca atatagtttt
    16921 ttaggaaata atgagcaatt aggctaagta aatagaatat aggggtgcaa gatagatctg
    16981 tgcaaaggtg accaagcttc atttcttggg gtgcacgaca gttttgggca aaggtagtta
    17041 agcagcattt cttcgagccc aatagtcttc atcgcggtca tttcttgctt tttcttgcct
    17101 tttttgccta cttatgcaat aatgaattat aaggtggcaa tagtagaatt atcggagaga
    17161 ttatgaaaga acgagcagag agattaaagg taataaaagc catcatcaaa gagaatagag
    17221 tcgattcgca ggaaaagctg cttcagcttt tggaagatca tggctattcg gtgacacagg
    17281 caacactttc ccgagacttg aagtatttaa aggtaggaaa gctctcggac gggcattccg
    17341 gttattacta ttccatccct tcagaagaag aacgccgcga atcagagcgc cattatatcc
    17401 aggatttttt gagaggttat gtttcgatcg attggaatca gagcctagtt gttgtgcgga
    17461 ccttttcagg gcattcggat tcggttgcgc ttgcaataga caatatgggt ttcgatgaag
    17521 tgattggcac gatatccggc agagataatg ttgtttttat cgcgctcaag aagggtgtga
    17581 gcggagatga ttttatccgc tcgctaaagg caagagtccc agaattcgag gaagaataat
    17641 cgacgtttat ttaattcata gccacttgac gatacttaga tggtcgctag gcgccgccta
    17701 gcaattttca taatttcatt tgcctaatgt gtgtctgaat ttgtatactt aataatgaaa
    17761 aatctctttt caaccacaaa ggaaaaatac tatgaattat gaacaattta ctctaaaagc
    17821 tcaggaagcg ctccgcgatg cggcttccat agctcagaag aatgatcaca gctctataga
    17881 acctgaacat cttcttttgg ctctgttgga tcaggagggc ggagtagtct cttctatttt
    17941 ggagaggttg ggagtagatc gggccaagct ggaacaaagg ctctccgatt tactaaaaac
    18001 cctaccaaag gcctatggcg aagccactca ggtctatttt tctccagcaa tgcagaaggt
    18061 catagccaaa gccgagtccg aatccgaatc gatgaaggac gaattcgttg cgagcgagca
    18121 ccttttctta gcacttttga tcgtcgagtc caaggctgca tctttactta gatcgatagg
    18181 agttactcac gaaggagtgc tcgcagttct gaagtcaata agaggttcgc agactgcttc
    18241 tgacgaaaat gcagaagaaa agtaccaggt tttggaaaaa tatacccgag atctaaccac
    18301 ccttgctcgt gcaggcaagc tcgatcctgt aataggtcga gatgaagaga tccgcagagt
    18361 aatgcaggta ctttcgagaa gaacaaagaa taatcccgtg cttattggag agccaggcgt
    18421 aggaaaaaca gctattgtcg aaggcttagc gcaacgcatc atatcgggcg atgttccaga
    18481 cagtctaaag gataaaaagg tccttgcact cgatttggga gccatagtgg ctggaagcaa
    18541 atttcgtggt gaattcgagg agaggctcaa ggccgtcata accgagataa ccaaatctga
    18601 aggcaagatc atactattta tcgatgaact gcatactttg gtcggagccg gagcggcgga
    18661 aggagccatg gatgcatcga acctattaaa gccagcactt gcacgcggag aactccgcgc
    18721 aataggcgcg actactttag atgagtatcg caagcatatc gaaaaagatg cggcgctaga
    18781 gcgcaggttt cagcctattt attgtgcccc tcccagcgta gaggatacaa ttgccatttt
    18841 gcgtggcctt aaggagcgct atgaggtcca ccatggcgtt cgaattcgtg acgatgctct
    18901 tattgcggca gcagttctat cggatcgcta tattacctct cgatttctgc ccgataaggc
    18961 catagatctt gtcgacgaag cagcctcgag gctcaagatg gagatagaga gccagccgac
    19021 cgagctcgat cagattcagc gcagattgat tcagcttagg atggaggcag atgctctcaa
    19081 aaaggagacg gatcctgctt ccatagaaag acgaaacaaa ctcgaaaaag agatagccga
    19141 gctctccgaa acacgcgatg aaatgactgc tcgctggcag caagagaagc ggttgatcga
    19201 ggaaattcgt caacttaagc agcagatcga agaatacaaa attcaagttg atcaacttgt
    19261 gcgtgaaggt caactcgcca aggcatctga gatcaaatat ggcaagctcg tcgaggctca
    19321 gcaaaagctt gaggaattat ccaagaaagt ccaagaagaa gaaggcaaac caagacttct
    19381 gcgcgaagaa gtctccgacg aggacatagc caaggtcgta tctctttgga caggtattcc
    19441 tgtttccaag atgctttcga gcgaaataca aaaatatgtc gagctggaga gccagttagg
    19501 caagagagta atcggtcaag aaagagcgct gcaagccgta gcggatgcaa tacgccgcaa
    19561 tagggcaggg ctttcggatc cgcgccgacc tctaggttct tttctctttg ttggaccaac
    19621 cggagttggc aagacagagc ttgcaaaagc gctggccgaa ttccttttca atgatgagcg
    19681 ggctcttact cggatcgata tgagcgaata tatggagaag cactcggtat ctaggcttat
    19741 cggcgctcct cctggatacg ttggctacga ggaaggtggt caactcaccg aggcggttag
    19801 aagacgccct tattctgtcg tattattcga cgaaatcgaa aaagcccatc ctgaagtatt
    19861 caatgttctt ctacagcttc tagatgatgg acgccttaca gatggtcaag gaagagtcgt
    19921 agattttcgc aatgtcatca tcatcatgac gagcaacata ggctcggagt tcattctcac
    19981 catgaaaagc atggacgaaa ttcgagatcg gatcgatgga cttcttaaga catacttcaa
    20041 gccggaattc ctaaaccgca tcgatgagat tgtcgtgttc gatcggctca atgaatccat
    20101 gatccgcaag attgctcagt taaggctcag tgaactcgga gagcggctta cgcagaggaa
    20161 tatcaagctt gaagtggaag acaaagccat cgactatatc gcaaaggaag gatatgacga
    20221 acaatttgga gcgcgccctc ttaaaagggc aatccaaaac ctacttgaga atcccattgc
    20281 caagaagatt cttgctggcg aaatcatgga aggcgacacc attcatgtca gtctatctgg
    20341 cagcgagctt gccatcggca ccaagcactc ttcagaataa aatactttag cttaaagtat
    20401 ctagggtttt agcttttgct gtatgcgccg agcctctttc gataatatga gatcgggtct
    20461 tatatattct gaatgaggtt cggtgtttcc attttgtact tcttttacag tatttcggag
    20521 cactcttatg aggtttgagc tattttctcc aagcgctgca aatagtcttt tatgtgaaat
    20581 atcttttcca aggcctctta ggtcggccat gatatctatt ccttgaatct cgcgtgctct
    20641 ttccagcgcg agcctgcttg tacaggcaaa aatcaagatt gtgagttttt tttcttctat
    20701 ttgtctcagt acagagagag tctgctcgag cctatctcca ggaagattca tattgtctac
    20761 atcgaagatt tcaagggcgt tttcgggaat cattccgact tgatctaaag attgcttttg
    20821 cttattgtcg acagtagctc gtagatagcc ttctctaaag gcttgattga atacctctag
    20881 ttccttatcg ctccttccta taccgcgagc aaagagcagt gctgcggagg catctttcgc
    20941 cgtctccttt ctgagagcag caatgcgata gcccgttaag agtccgagct gttggtatgc
    21001 ccaagcgtta tcccattcaa cagaacaaaa gccatttgct ggagtcagaa ggttaattac
    21061 cgcaaagcga tcgtcggatg caaattcttg aaatgctata gctacagatg gactcgttat
    21121 aaagatattc aaagattgat tgtctgtcgt cttcttttct attttctttt ccaaaagctg
    21181 ttgagcgact ttagggtttg ccacattctc tagcgggaat tctcttgcaa cagcttgaga
    21241 gagatccgga tagagaagca taaaagcttc atcaaaaact gcttgtgccg ctggatgctt
    21301 gcagcttgag aataagaggc ttatcagtat aatagaaata tacatcaatg tgcgaggaag
    21361 tctccggcag aaagaagcta agcaatactc aattgtgtac cacatttgat aaacctgttg
    21421 cataatcgca taattctcta tataacgaca ataacctgct gcaaagagaa tttgtacatt
    21481 gtcggaggat tatatgatca gttataaaga gcttggcctt gtaaacagtg ttgagctttt
    21541 ccgcaaggct atggagggtg gatacgctat cccagcctat aattttaaca acatggagca
    21601 gatgcaggct attattcagg cttgtgttga gacgagaagc cctgtcatac ttcaggtatc
    21661 ctctggtgca cgcaagtatg caaacgctac cctactgcga tcgatggcaa aaggcgctgt
    21721 cgaatatgca aaagagctag gctatcctat tccgatcgta ctgcacttgg atcatggtga
    21781 tagtttcgag ttatgcaagg actgcataga aaacggcttt tcttccgtaa tgatagatgg
    21841 ttctcatctg ccttacgatg aaaatgtggc tcttacaaaa agagtggttg aatttgccca
    21901 tgctcagaaa gactatgtca gtgtggaagg agagcttggg gtgcttgcag gtatcgaaga
    21961 tgaagtatcg gcggaaagga gccattacac aaggccagaa gaagtcgaag acttcgtcaa
    22021 gaagacggga gtcgatagtc tcgctatttc gattggtaca agtcatggtc gtgcaaagtt
    22081 caaaccagag caatgcactc gtactcctga tggagtgctg attcctcctc cgcttcgttt
    22141 cgatatcctc gaagaagtgg aaaagcgaat tcctggattc ccaattgttc ttcatggttc
    22201 ttcctcggtt ccagtagagt atgtgcgcat gatcgagcag tacggtggga agcttccaga
    22261 ctctgtgggc attccagaag atcagcttag aaaagcagct cgcagtgcgg tatgcaagat
    22321 caacatcgat tccgatggaa ggcttgccat gaccgctatt gtgcgaaaag ttcttttcga
    22381 gcatcctgaa gagttcgatc cgcgcaaata cctggggcct gcacgagatg agctaaagaa
    22441 gctttacatg cacaagaaca ttgaagtgct gggttccgca aaccgcgctt agtatcgcga
    22501 ttagtattta gagtagatta ttttttagtg aacatctctg taggccttta cctacagggg
    22561 tgagtatgat aatatacgcc gcgcctttga gctgtcgaaa aggcagagaa aagaggccgc
    22621 ttttctgccc ccgttaagag aagcgagcct gcggcagagg aggccagttg gcagagaaag
    22681 atttgcggat caacgagcag atccgtgtgc gcgaagtgcg tctaatcgat gaaaatggcg
    22741 agcagagagg aatagtacct actcttgagg cattgcggat cgcacatgag gccggactcg
    22801 acctcgtgga agttgcgccg caatctgttc cgcctgtatg tagattgctc aactatggca
    22861 agtacaagtt tgagcaagaa aaaaaggtaa aagatgcaaa aaagcgagca aaggtcacgg
    22921 aact
//
LOCUS       DAOSOB010000029        21918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_2376_length_21918_cov_79.436948, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000029 DAOSOB010000000
VERSION     DAOSOB010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 21918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21918
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2376_length_21918_cov_79.436948"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLS88_04575"
     CDS             <1..608
                     /locus_tag="PLS88_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU06653.1"
                     /translation="LSAIDHYDVTKGYHFISYAVWWIRQAILKAICEKSRMIRLPLNR
                     ANELVQIEKARALFEGDISEEGEIREVAKFLNMEPEHIKDIISISRELISLDAPVYDE
                     RNSTTVGELIENEKHESPEQNAIQSSLRDDINKVLSTLTAKEREIVEYRFGLNGKRPM
                     SLKEIGDRMHLTKERIRQIEKAALKKVAVPETMEMLQDYVA"
     gene            696..1862
                     /locus_tag="PLS88_04580"
     CDS             696..1862
                     /locus_tag="PLS88_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU06654.1"
                     /translation="MQTSDEIFFQDFAQKSKDHISKRIVGIFLENKELFDTLPELGKW
                     LSDEMQDFACSGKMLRGTLAFVGSRLFHEEGISITDFIDDKVRSVSASLELFQAGLLV
                     HDDIMDHDQMRRGKPTFHLRIKNLLEERRPNCNITSAFAIAEAQGICVGDLFFFLGWQ
                     EISKLDFNISSLFARELALVTLAQIRDVGFGYSNSYPDINEVIEVYRHKTARYTIVLP
                     LIAGAIISQSSVSHASIANASTSQGTQRMEKSIFSLLEAFGESMGIVFQLQDDRLGLF
                     GNEEETGKPIGSDLKEGKKTPYIILLVNRLEKKERERFDSIFASPKQELKDIEWIRKI
                     VIAKGVEADIQRIIDSYLLKAKTALNELSALSSIETKTISLLEGFMQYSGKRSS"
     gene            complement(1992..4421)
                     /gene="gyrA"
                     /locus_tag="PLS88_04585"
     CDS             complement(1992..4421)
                     /gene="gyrA"
                     /locus_tag="PLS88_04585"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HOU06655.1"
                     /translation="MAEPSNIIPVPIEEEVKSSYLNYAMSVIVSRALPDVRDGLKPVH
                     RRLLYAMDELGLRSNAPTKKSARITGDAMGKYHPHGDQSLYDALVRMAQPFSLRYPLV
                     QGQGNFGSIDGDPPAASRYTEAKLSKIGEEMLADLDKETVDFVPNYDESLKEPSVLPS
                     AIPNLLINGSSGIAVGMATNMPPHNLREIALAIEAFIDKPDITIDDLMNYVKGPDFPT
                     GGIIYGLQGIKDAYETGRGRLTVRGRFIIETMKSGREQIVFTEIPYALNKTILVTRIA
                     ELVREKQIDGISDLRDESDREGIRIVLELRKGAITKIVLNQLFMRTPLQSTFGVINLA
                     LVDGVPKCLNLKELIFYFVQHRFEVITRRSKFELKKAEERAHILRGLVIALRNIDEVV
                     AIIKSSQNIDTAKNRLCERFELSEAQAQAIVDMRLGRLTSLETDKILAELKDIEARIE
                     YLKGLLSDPNSIRLVIKQEIHDLAEKYGDDRRTEIVPNQVEQINIEDLIKPEEMAILI
                     SNKGFIKRISVNQYRSQGRGGKGSNSAALIEEDFVEQLFIANTHDYLLFISSWGKAYW
                     LKVLEIPESSKQARGPHIRSLLAISEDEEITAVVDFSDFSEKEYILMGTLKGVVKKVA
                     TKEFVNAKTRGIIGISLDNGDKLISAILTQGSDEVMLISRKGQALRIHESQVREMGRT
                     ARGVRGISLHESDELAAMLRVNPNESMLIITQNGFGKRVSYDLFNVHSRGTKGQTIYS
                     PDEFSGEVVKGVSVQEEDEVMVITSTGKTIKLNVSSVRQMGKGARGVRIVNIEPPDFV
                     IGIDKIVQEQE"
     gene            complement(4434..6350)
                     /gene="gyrB"
                     /locus_tag="PLS88_04590"
     CDS             complement(4434..6350)
                     /gene="gyrB"
                     /locus_tag="PLS88_04590"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOU06656.1"
                     /translation="MESKTSSYSANTIQVLKGLEAVRRRPGMYIGTTGIEGLHHLVYE
                     VVDNSIDEALAGFCKNIVVALERQDIVRVEDDGRGIPVDIHPTEGISALELVMTRLHA
                     GGKFDKKNYKVSGGLHGVGVSVVNALSAWLEVYVHNNGKIFYQKYVRGIAEKPVAAIG
                     ETDKSGTVVRFQADPDIFEETVYSFDVLSNRLRELAFLNKGIKISLIDERLSQPKRID
                     FHFEGGLQEFVEYLNKNKTVIHKDVIYFLGTRDEVEIEIGMQYNDGYNETMFTYVNGI
                     NTKEGGTHLVGFRNALTKVVNDFFKKSKYSKKIEETLSGDDVREGLTAVLSIKVLEPQ
                     FEGQTKAKLGNTEVKGIVEGFLSEQLDLYFQKNPDVIDIILGKCTSAARARIAARQAR
                     ELTRRKNLLEASSLPGKLADCQEKDPTKCELFIVEGDSAGGSAKMGRDRLFQAILPLW
                     GKMLNVEKARLDKVIGNDKLQPIIASLGTGIGEDFDISKLRYHKVIIMADADVDGSHI
                     RTLLLTFFYRYMTELIEKGHVYLAMPPLYRISFDREIHYAYSDEEKDRIILQANKDPS
                     KISVQRYKGLGEMNPEQLWETTMDPSQRIIKRITMEDAILAEEMFVTLMGEQVEPRRR
                     FIEENALTVSNLDI"
     gene            6778..8169
                     /gene="dnaA"
                     /locus_tag="PLS88_04595"
     CDS             6778..8169
                     /gene="dnaA"
                     /locus_tag="PLS88_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOU06657.1"
                     /translation="MTDDNGTIWQKALEIAKSQVPESEFIMWFRLSYVGFENGTILLE
                     ANNTFLRDQFVRKYSQFMKEIVSSLLGMPVELSVDAQSRVIDHNISPQAAPKKAKSDI
                     ATEKVITTKPDNSRSSRSANLQPRYTFESFVVGENNNFAFNAASAVADKPGTSYNPLL
                     IYGGVGLGKTHLMHAIGNRIVEKHGDFKIICVTAEEFTNEFIKTIHDRTTNDFKNKYR
                     NVDVLLIDDIHFFQSKHGVQEELFHTFNALYDSNKQLVFTCDRPAAELKDFSERLKSR
                     FMMGLKTDLTTPSFETRAAIVRKKLELYNRDFSEDVINLIAKSIETNVRDLDSCINQI
                     IAYADLIKVEPTFEIAQNVIKQLTNSFMPPAIDVSSIIKTVADYYKLSISDLKGKKRS
                     KNIALARQIAMYIIRETTDYSTTEIGSEFNGRDHTTVMHSCQKIEDLVKFDASFASVV
                     NRLIKECKEKSSS"
     gene            8482..9582
                     /gene="dnaN"
                     /locus_tag="PLS88_04600"
     CDS             8482..9582
                     /gene="dnaN"
                     /locus_tag="PLS88_04600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOU06658.1"
                     /translation="MRFVCDRDTFAKEIAIAQDIIASKNAFSIMSNVYLEVVDSRLII
                     KATDAKVGFETEIPISNGEPGALTVFCDKLMAITNSIPEGDMILEQHDSLIEIKTLSR
                     NVRFQLKTIAGDKYPVIPDIEENQYFPLKARDLRKMISQTIFSVSNDETRFFMNGVFI
                     EKLPENVLVMVATDGRRLAYISIEEGNIPDFRPAIIPPKVLSVILKRASDEGQIYMAI
                     SEKNVFFRFGNYELTSYLIEGRFPNYQKVIPQNQRQKFTVSKDEMLSALRRVSLFVER
                     SNKVIFDISRKGLVIKSEETELGAAREELACEYDGDDVVIFLNLRYLEDPLKVMDSEN
                     ITIEFSDPTRAITLRPEPASNYFHVMMPIQQP"
     gene            9583..10710
                     /gene="recF"
                     /locus_tag="PLS88_04605"
     CDS             9583..10710
                     /gene="recF"
                     /locus_tag="PLS88_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOU06659.1"
                     /translation="MAFEHIRIAQFRNIEEAELELGSGNIFLLGENGQGKTNFLEALY
                     CLSYGNSFRTHQDRELIMHGRSDFLLRAEWSEPGYIVRDQISFRYLPEGRKEILLNEK
                     QLSDRKELVAHNPSVVFCHEDLAFASGAPEERRFFFDQCAGMLWLEYIDLLRSFRRLL
                     KHRNMALKTDTFSVLDTLDEQLARYGSELMLYRKRLSHLFKDHFPERYEQVSQLGTEV
                     SIQYRPNWPEEASLSEIVEILAQRRQRDIEVGTTLSGPHRDRWVFTMEGRDFSAFAST
                     GQLRLASLVLRVVQVYLFSELSQSAESAEPRFPVLLLDDVLLELDVAKRRRFFSLLPA
                     KDSGAQSVFTFLPEEPWREYANRSTIVYKVSHGRFSREESI"
     gene            10694..11011
                     /locus_tag="PLS88_04610"
     CDS             10694..11011
                     /locus_tag="PLS88_04610"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOU06660.1"
                     /translation="MKKASEILSRFFDDNMFEAATQFAAFRSTWKQIVGERLADHSKP
                     KGIMHHCLIVSTEHSGWIQLLQLNQEKILARISKNYPELDITGLAFTIEDNKEASSTT
                     DRA"
     gene            11143..11298
                     /gene="rpmH"
                     /locus_tag="PLS88_04615"
     CDS             11143..11298
                     /gene="rpmH"
                     /locus_tag="PLS88_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011772387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOU06661.1"
                     /translation="MKRTYQPSRVKRNRKFGFRARMKTKSGRQILSRRRAKGRIKLSV
                     SDEKKKY"
     gene            11279..11671
                     /gene="rnpA"
                     /locus_tag="PLS88_04620"
     CDS             11279..11671
                     /gene="rnpA"
                     /locus_tag="PLS88_04620"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOU06662.1"
                     /translation="MRRRNTENTQSGIQEERQDRSFPKSLRVRKTKEIDLIFEKGARF
                     SCKGMRMHVLLKEGQESRVAFIAGRAFSGAVTRNRAKRLAREAWRHIRNSVKPGFDIV
                     FVLYPGLETFVEFYSAMQYLLRKADLLL"
     gene            11668..11898
                     /gene="yidD"
                     /locus_tag="PLS88_04625"
     CDS             11668..11898
                     /gene="yidD"
                     /locus_tag="PLS88_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOU06663.1"
                     /translation="MNIVKQIFSFPLLIAIRLYQLFISPLFQPSCRFYPTCSSYSYEA
                     IHKYGPLKGLLLAFKRIIRCHPGNPGGYDPVP"
     gene            11895..14006
                     /gene="yidC"
                     /locus_tag="PLS88_04630"
     CDS             11895..14006
                     /gene="yidC"
                     /locus_tag="PLS88_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOU06664.1"
                     /translation="MNEINEQQTTSKEERKRMILAVVLSTVIVSVGFIVQNILFPPAT
                     QQQTTQSSEQTGANGQAIPQEPSSATPTKALPSIIPISTENAPIANMIPVPTTEERYI
                     VETDVVRATFTNAGGELVSLTLKKHKDLRDSRGGVDMVVAGSQGADGLSVSFGQSSTP
                     MRELMNAQYLDESKNTIAFSRMFEAKMAGSDEKVPFIFKKVFSFRDGEYLFGLAISIE
                     QPEGKPIILGTDGVAYRIDLGPQIGPRFDQMPKNADYRKYIAEIDGKKKNQKLKPNTS
                     DTIAPNASWVALSGKYFTFIAVPQAPLSAYEIAASQDPLINQTNSLGLLRSTFSGASV
                     TDTYYFYFGPKTSAELGKYEYADKNGFGLSNLKLEDAMEGSGILGWLENILKFLLNVF
                     YRLIPNYGVAIILVTVLIKLLFYPLTKKGSLATARMSELQPKMQELQAKYKNNPQKLN
                     QEMAELYKREHYNPMSGCLPMLIQFPLFIAMYNLFNNHFELRGAMFIPGWINDLSLPE
                     SLVNFGNFKIPLLGWNDLRALPIIYLFSQLLYGKFTQAPQSTQSNNPQANQMKFMMYG
                     MPIMFFFILYDVPAGLLIYWITNNILTIAQQVLINNLMKKRKIASAEITAGGMPAVGA
                     ASAGPRLATGKSSAIDTKAKTVGREPTKEAFSERVTKWLEKQAAEAEKASGSASSRKK
                     GEGRSYSSDRSSVTRKNKAKK"
     gene            14039..14791
                     /gene="jag"
                     /locus_tag="PLS88_04635"
     CDS             14039..14791
                     /gene="jag"
                     /locus_tag="PLS88_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOU06665.1"
                     /translation="MIYEFEGKTEREAMELATQELGLDTSSFDVEIIENQSGGLFKKG
                     KVRIRVHTKDSSFAENRARESNEYNEFEEKQEAREARETREAREERHIEPLPMDDFES
                     KIVEWTTEVINLMGYSAQVSVAFREPNKLGLRIDTDSASILIGKKGRNIDALQLLANV
                     YAGNLGHSDTKIVLDSENYRLRREEALVRIAYKTAEEVRSTGHSILLEPMNPFERRII
                     HTTLNDIIDIETKSEGDGLIKQVRVMLKGRNQ"
     gene            15120..16481
                     /gene="rsxC"
                     /locus_tag="PLS88_04640"
     CDS             15120..16481
                     /gene="rsxC"
                     /locus_tag="PLS88_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOU06666.1"
                     /translation="MSIKTFRGGAHPSERKDRSQHAPIEKVSSVPQVVIPVNQHFGAP
                     IQPLIKVGDFVRLGQKIADAEGRMTVPIHASIAGVVKKIEPRMQSNNIEGLCIVIEPK
                     DTPEGESDREFMPPLDPFACRKEEALLRIREAGIIGMGGAGFPAHVKLAPTKPISLVI
                     ANAAECEPYLTIDERTLLEMPDKIVDGLSIVMKIINASEGIIAIEENKKYTIPLVQKA
                     IEENPQVKAGMKIGIQVLKTKYPQGGEKMLITAITGRQVPSGGLPMDVGCVVSNVGTL
                     CAIADAFREGKPLIERGFTVTGGACKTPKNLIAPVGTVVADLAKSGIIDIDEESLSKV
                     IFGGPMMGVAVPSTEIPVQKNTSGVLLMSVEEALRFEEKTCIRCSRCIRACPMKISPA
                     LLAIAIESGNYGEAEKIGLQDCIECGACSFVCPAHRYLVQRFRVGKQVLRALKQKEAQ
                     NVR"
     gene            16471..>17150
                     /locus_tag="PLS88_04645"
     CDS             16471..>17150
                     /locus_tag="PLS88_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOU06667.1"
                     /translation="MSDKKIILSSSPHTFSPVDTPRIMLSVVIALVPAAAYGIYLYGL
                     AALGVILTSITAAVLSEFLFRKLIKASTTVGDFSAVVSGLLLALVLPPATPLWMVALG
                     AVFAIVVAKEFFGGLGANPFNPALIGRAFLLMSFPAAITSWNMPKGLALPADAVSTAT
                     PLNLIKQGNSLADVAKYFGASDIGAFYRQLFIGYRPGTIGESSILLVLLGGLFLLAIG
                     VIQWIVPVS"
     assembly_gap    17151..17250
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17251..17636
                     /locus_tag="PLS88_04650"
     CDS             <17251..17636
                     /locus_tag="PLS88_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOU06668.1"
                     /translation="GGLFLLAIGVIQWIVPVSVLASTFVFSWLLGMDPVFSLLTGGIA
                     FGAFFMATDYATRPLTEKGQALYGIGIGLIAVLIRKFGGYPEGVTYAILIMNVLTPFL
                     NKLRTKKYGFLPPPKPVKPSKEATK"
     gene            17633..18361
                     /locus_tag="PLS88_04655"
     CDS             17633..18361
                     /locus_tag="PLS88_04655"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOU06669.1"
                     /translation="MKKTKLGLILMLYASIACASLAVVYAFTKDSIERQSEIQLQASL
                     KDIFPQATAFDVVENLTSPEPNIKFETAYEVKSDNVPLGLAIKVSGPSYGGNATLLVG
                     IDLHRSIAGVRIMALNDTPGLGMNASNPTYYVDKAKKVTFLGQFEGKFLTDPFEVKKD
                     VAAITAATITSKSLTKLIKTAGDAAIAYMDQKVMMASMEDEKPTGETSIGASPAVSTG
                     SPIGAPGASAGTAKESARPQAGGK"
     gene            18364..19005
                     /locus_tag="PLS88_04660"
     CDS             18364..19005
                     /locus_tag="PLS88_04660"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOU06670.1"
                     /translation="MKKLYSVFKQGIIFDNPLLMLMIGLCSAIAVTTNVSNGIGMGLA
                     MTFVLLFSEIIISIFRNLIPSMIRMPVFIIVIAAFTTIVDLMMKAYFPDLSKSMGVFI
                     PLIVVNCIIMGRVEAFASKQNLPSAIADALGMGLGYTWVLIGLAAIRELLGNGTFAGI
                     QVMPASYEPILFFILPPGGFFVFSLFISFNIWLKKKMEASARKKELVSKKEAA"
     gene            19005..19580
                     /locus_tag="PLS88_04665"
     CDS             19005..19580
                     /locus_tag="PLS88_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOU06671.1"
                     /translation="MDLGKIFLLSMLVNNIVVMRFIALCSYIGMTSDLGQSMGMGYAV
                     TFVTVLTTAATWPIYHYILVPLNLTFLQILVFILVIASLVQLVEFYLKKNIPGLYSAM
                     GIYLPLITTNCAISAVTFENISNNYNFIQSIVYAIGVSLGYLLAMILLAGIRDRMKTS
                     PVPKFLQGTPILFMATSLIGIAFMGFTGLIK"
     gene            19593..20444
                     /locus_tag="PLS88_04670"
     CDS             19593..20444
                     /locus_tag="PLS88_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOU06672.1"
                     /translation="MLITILLTIGFSAALAFILGVALGFFNEKFKIERDPKISEVRQA
                     LPGANCGACGYPGCDGYSEAVATGRAPTTKCSVGGSATAEMISKIMGIEAGTVEEKVA
                     VLLCQGSIDKTAKKGEYSGIPTCRAAKLSTGGIKACAWGCQGFGDCVLVCKFDAIEMG
                     EDGLPHIDYDNCTGCGLCVEECPQKILTLIPLTKAGSIVLCSNRTVLKQTVLKNCKVG
                     CIKCDLCVRSCPEGAISMLNGIPVTDYAKCNSCGICVQKCPTKCYKLLETEVFGTKKL
                     IPVMEEA"
     gene            complement(20529..21671)
                     /locus_tag="PLS88_04675"
     CDS             complement(20529..21671)
                     /locus_tag="PLS88_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOU06673.1"
                     /translation="MVALFRKRQAALLQAAIRWAAILFFLSSVPALQSAFAYVSVPPL
                     AGLVSSGARQAVLRTAESYLGTQYRYGGSSKSGIDCSGLVYISYLKATGVKIPRSVDA
                     LVKWVLIIPKNELEPGDLVFFSLEAKTNSFSKSSSANVSTSVPSGLGYFNIGLSSLAA
                     STLSKADHVGIYLGDDIFIHATSTGAKTGVIHSSLREKNWQQRFLFAGRALPASGFSG
                     FASDWGGAISFAQLDTLLQGDLLAFVRGVSGWTEISLPIAKNFNAGLRAGAQWDRTLS
                     VVRVPIELDLGQISGISVFAGPAFTFGSPALDDRLYAPGDFFLATGGLRWSSFFYSTG
                     AHRLGSYIELRYDYYVPKQGQVESIRSDLQSCLSISIGLRLRNVHY"
BASE COUNT         6157 a         4743 c         4885 g         6033 t
ORIGIN      
        1 ttctgagcgc tatcgatcat tatgatgtta caaaaggcta tcattttata tcttacgcag
       61 tttggtggat acgccaagca attctcaaag cgatctgcga gaaaagccgt atgattcgac
      121 ttcctctaaa tagagccaat gaacttgtac agatcgaaaa ggctcgtgcg ctgttcgaag
      181 gagatatttc tgaagaggga gagatacgcg aagtagccaa gtttctcaat atggaacccg
      241 aacatataaa agatatcata tccatttcac gcgagctgat ttcgctagat gctcccgtat
      301 atgacgaaag gaattctaca actgtaggtg agctaattga aaatgaaaag catgaaagcc
      361 ccgagcagaa tgcgattcaa tcaagccttc gcgacgatat aaacaaggtc ctttcaacct
      421 tgactgccaa agagcgtgag atcgtagaat atcgctttgg gcttaatggc aagcgtccta
      481 tgagtctcaa agaaatcgga gatcgcatgc atcttacaaa agaaagaatt cgtcagattg
      541 aaaaggcagc cttgaagaaa gtggctgttc ctgaaaccat ggaaatgctt caagactatg
      601 tcgcttaaac tgtgagatgt aatttaatgt ttttagccct ggcatatcat tgctagggct
      661 tttttagtgc ataacagagc gctatactgc tctccatgca aacatctgat gaaattttct
      721 ttcaagattt tgcccaaaaa agcaaagatc atatttcaaa gagaatcgtg gggatttttc
      781 tagaaaacaa ggaattgttt gacactctac ctgaattggg caaatggcta tctgatgaga
      841 tgcaagattt cgcatgttct ggcaagatgt tgcggggtac gctcgctttt gtgggatctc
      901 ggcttttcca tgaggaaggc atttctataa ctgattttat tgacgataag gttagatcag
      961 tttctgctag cttagagcta tttcaggcag gactgcttgt tcatgatgat attatggacc
     1021 acgaccaaat gcgccgggga aagccaacat ttcacctgcg gataaaaaat ctattagaag
     1081 aaaggcgccc taattgcaat ataacctcag cttttgccat agccgaagct caaggtatat
     1141 gtgtcggaga tctttttttc tttcttggct ggcaagagat atctaagctc gatttcaata
     1201 tatcctctct atttgcaaga gaactcgctc tcgtgactct cgcacagatt cgagatgtgg
     1261 gttttggata cagtaatagc tatccagata tcaacgaagt aatagaagta tatcgccaca
     1321 agaccgctcg ctatacgatt gttttacctt tgatagctgg tgcaatcatt tcacaatctt
     1381 cggtgtctca cgcttcaata gccaacgcct cgacatctca gggaactcag cgaatggaga
     1441 aatcgatctt ttctttgctt gaggcttttg gagaatccat gggtattgtc tttcaactcc
     1501 aggatgacag gcttgggtta tttggcaatg aggaagagac agggaagcct attggttcgg
     1561 accttaagga aggcaaaaag actccatata ttattctctt agtgaatagg ctcgaaaaga
     1621 aagagcgcga gcgatttgat tctatatttg catctccaaa acaagaattg aaggatatcg
     1681 aatggatacg caagattgtc atagctaagg gagtagaagc agatattcag aggataatcg
     1741 attcatatct gctaaaagcg aaaaccgccc ttaatgagct ttctgctttg tcttcgatag
     1801 aaacaaagac tatttctcta ttggaaggct ttatgcaata ttctggcaaa cgttccagct
     1861 aagaaaggta catatccaag agctttgccg ttatcaacat tgtttcacgt gaaacaacac
     1921 agacattctg gatagcgcta aaaacgacag ttcgattcac tttcttctaa tgtttccttt
     1981 ttatggctgt tctattcttg ctcttgaacg atcttatcta tacctatgac aaaatcaggc
     2041 ggttctatat tgacaatcct tacgccacga gcacctttac ccatctggcg tactgaagac
     2101 acattgagct ttattgtttt gcctgttgaa gttatgacca taacttcgtc ttcttcctga
     2161 acagacacgc cctttaccac ttctccagag aattcgtctg gcgaatagat tgtttgtccc
     2221 ttagtaccgc gcgaatgaac attgaaaaga tcataactta cacgtttacc aaatccattc
     2281 tgagttatga tcagcataga ctcattgggg tttactcgca gcatagcagc aagctcgtct
     2341 gattcatgca aggaaattcc tcgcacaccc cgagcagtgc gacccatttc tctaacctga
     2401 gactcatgga ttctcagcgc ctgacctttt ctcgatatca acatcacctc atcggagccc
     2461 tgtgtcaaaa tagcggatat cagtttgtct ccattatcga gagaaatacc aattatgccc
     2521 cgcgtcttgg catttacaaa ttcctttgtt gcgactttct taacgactcc tttgagtgta
     2581 cccatcaaga tatattcttt ttcggaaaaa tcgctaaaat cgactacagc cgttatttct
     2641 tcatcttcgg aaattgcgag aagtgaacga atatgagggc ctcttgcctg cttgctcgac
     2701 tctggaatct cgaggacttt tagccaatat gctttgcccc agcttgaaat aaagagcaga
     2761 taatcatgag tgttggcaat aaatagctgt tcgacaaagt cctcctcaat taaagcggct
     2821 gaatttgagc ccttcccccc tcttccttga gacctatatt gattcacaga gattcgctta
     2881 ataaaaccct tgttggagat gagtatagcc atctcttcag gtttaattag atcttcgatg
     2941 ttgatctgtt cgacttgatt gggtacaatt tcagtgcgtc ggtcatcgcc atatttttcc
     3001 gctagatcgt gaatttcttg cttgatcaca agacggatgg aattggggtc tgaaagcaat
     3061 ccctttagat actcaattcg ggcctcgata tcctttagtt cggctagaat tttatctgtt
     3121 tcgaggcttg tcagccgacc gagccgcata tcgacaatag cctgagcttg ggcttcgctc
     3181 aattcgaagc gctcgcataa gcgatttttt gctgtatcaa tattttggct tgattttatt
     3241 atggccacaa cttcatcaat atttctcaaa gcaattacca ggccacgaag aatgtgagca
     3301 cgctcctcgg cttttttaag ttcgaatttg gaacgacgag tgattacttc gaacctgtgc
     3361 tgaacaaaat agaaaattag ctctttcaaa ttgagacatt ttggtacgcc atccactagc
     3421 gccaggttga tcactccgaa tgtgctctgt aaaggcgtcc tcatgaatag ctgatttagc
     3481 acaatcttgg ttattgcccc ctttcggagc tcaagaacta tgcggattcc ttctctgtcg
     3541 ctctcatcgc ggaggtctga aattccatcg atttgctttt ctcgcacaag ctctgcaata
     3601 cgggttacaa gtatcgtttt gttcaacgca taaggaattt ctgtaaagac aatctgctcg
     3661 cggccacttt tcatcgtctc tatgataaag cgtcctctta ccgtcaacct tccacgacct
     3721 gtctcgtagg catctttaat accttgtaag ccgtagataa taccgcctgt cgggaaatcg
     3781 ggccctttga cgtaattcat taaatcatca atagttatgt ctggcttgtc gataaaagcc
     3841 tctattgcga gcgcaatctc gcgcaagttg tgtggtggca tattcgttgc cattccaacg
     3901 gctatcccgc tcgagccgtt tataagcaga tttggaatgg ccgaaggcaa aacgcttggc
     3961 tcttttaagc tttcatcgta gttgggaaca aaatccacgg tctccttgtc gagatcagca
     4021 agcatctctt ctccgatttt cgagagctta gcctctgtgt agcgactagc cgccggagga
     4081 tctccatcta tcgaaccgaa gtttccctgg ccttgaacaa gtgggtatcg caaggagaaa
     4141 ggctgagcca ttctaacaag agcatcgtaa agcgactgat ccccatgtgg gtgatatttg
     4201 cccattgcat cgccggtaat acgcgcactt ttcttggttg gcgcattact gcgaagtcca
     4261 agttcgtcca tggcatacaa aagccgccga tgtaccggct tgagtccgtc tcgcacatcc
     4321 ggtagagcgc gcgagacgat tacggacatg gcatagttga gataagaact cttgacttct
     4381 tcttctattg gaacaggaat aatattgctt ggttccgcca ttctgatctg cctttatata
     4441 tctaggttcg ataccgtaag cgcattttct tcgataaaac ggcgccttgg ttccacttgc
     4501 tcgcccatca gggttacaaa catctcttct gcaaggattg cgtcttccat ggtaattcgc
     4561 ttgatgattc tttggctggg gtccatggta gtttcccaaa gctgttctgg attcatttcg
     4621 cctaagccct tatagcgctg taccgaaatc ttggaagggt ctttattcgc ctgcaatata
     4681 atcctatcct tttcttcatc gctataagca tagtgtattt ccctgtcaaa acttatgcgg
     4741 tataggggcg gcatggcgag gtatacatgc cctttctcga tgagttctgt catatagcga
     4801 tagaagaaag tgagaagcag ggtccttata tgagatccgt ccacatcggc atctgccatt
     4861 atgatcacct tatggtaacg gagtttcgat atatcgaaat cttctcctat gcccgtgcca
     4921 agactagcga ttatcggctg tagcttgtcg ttgcctatca ccttgtctaa tctagctttt
     4981 tcaacattta gcatcttacc ccatagaggc aaaatggctt gaaaaagcct gtctctgccc
     5041 atctttgcag atccaccagc agaatctccc tctacaatga aaagctcaca tttggttggg
     5101 tctttttcct gacaatctgc tagcttgcct ggcaggctcg atgcttctag gaggtttttt
     5161 cgtcttgtta gctcgcgggc ttgtcttgct gctatgcgag cccgggctgc gctagtgcat
     5221 ttccctagga taatatcgat tacatcggga ttcttttgaa aatagagatc aagttgttcg
     5281 gatagaaagc cttctacaat acccttgact tcggtgttac ctaacttggc ctttgtctgg
     5341 ccttcgaatt gtggctccaa cacttttatc gatagaaccg cagtaaggcc ttcgcgcaca
     5401 tcatcgcccg aaagagtctc ctctattttc ttgctatatt tagatttctt gaaaaaatcg
     5461 ttgacaactt ttgttagagc atttctaaat ccaacaagat gggtgccgcc ttctttagta
     5521 tttattccat tgacataggt gaacatcgtc tcattgtagc catcgttata ttgcatgccg
     5581 atttctatct caacttcatc gcgggtaccc aggaaataga tcacatcttt atgtattacg
     5641 gtcttatttt tattgagata ttctacgaat tcctgaagac ctccttcgaa gtggaagtca
     5701 attcgtttag gctgagacag cctttcgtct ataagagata tctttatgcc tttgttcagg
     5761 aatgcaagtt ctcgtaagcg attgcttagc acatcgaatg agtaaactgt ctcttcgaat
     5821 atatctggat cagcctgaaa tcgtactacc gtacctgact tgtctgtttc tccgatagcg
     5881 gcaactggct tttccgctat gccgcgcaca tatttctgat agaatatctt tccattgttg
     5941 tgaacgtata cctctaacca ggctgacagc gcattgacca cagagactcc tacgccatga
     6001 agccctccag agaccttgta attcttcttg tcgaatttgc cgccagcatg aagacgggtc
     6061 ataacaagct cgagggcaga aataccttca gtaggatgga tatccaccgg aataccccgg
     6121 ccatcatcct ctactctcac aatatcctgc ctctccagcg ctactacgat atttttacag
     6181 aagcctgcca gggcttcgtc tatcgaattg tcgacaactt catatactag atgatggaga
     6241 ccttcgatac ccgtagtacc tatgtacata cctggccttc tgcggacggc ctccaatcct
     6301 ttgagcactt gaatggtatt tgcggaataa ctgctagtct tagattccat ggttatcctg
     6361 aattgaatag tagaaaatag gagcaagatt tggcaaaaat ctcattgctt atcatatcaa
     6421 atagagggaa ttgagtaaag tcaaatgcca aagcgcttcg aaaaacctaa ttatattgac
     6481 ctgtggataa cctgtgcata acttgttatt tcgttgtgat tagcttgtgg atttataaga
     6541 taattccttt agcttacaag aaaatctgtc atttataatc gcaacttaaa ataatcaatc
     6601 aagaaatagg cctattatag gccactttta gattgtttcc ttaacaaaaa gcataaaatt
     6661 tttaaaattt gccctattgg catcttgcac tcgatggtgc tttggtatat agaaaattta
     6721 ttccttcttg tggaaaactt gtggaaatgt gcatggagga gctggagatt ctgacgcatg
     6781 accgatgata atggaacaat ttggcaaaaa gctctcgaaa ttgccaagtc tcaggtccca
     6841 gagtccgaat tcatcatgtg gttcaggcta tcttatgttg gattcgaaaa tggaactatc
     6901 ctgctcgagg cgaacaatac cttccttaga gatcaatttg tcagaaaata cagtcaattt
     6961 atgaaggaga tcgtttcatc ccttctggga atgcctgtag aattatctgt ggatgctcaa
     7021 tctagagtta tcgatcataa catttcacct caagccgcac cgaaaaaagc aaaaagcgat
     7081 atagccacag aaaaagtaat tacaacaaag cccgataact ctcgctcttc tcgctctgcc
     7141 aatcttcagc ctcgatatac ctttgagagc ttcgttgtgg gagagaacaa caattttgct
     7201 ttcaatgcag caagcgcagt agccgataag ccaggaactt cctacaatcc acttttaatc
     7261 tacggtggag ttggccttgg caaaactcac ctcatgcatg ccatcggaaa ccgcatcgtt
     7321 gaaaaacatg gtgatttcaa aataatttgc gttacagcag aggaattcac caacgagttc
     7381 atcaagacta tccacgatcg cacaaccaat gatttcaaaa acaagtatcg caatgtcgat
     7441 gtcttactga tcgacgatat ccattttttt caaagcaaac acggagtcca agaagaacta
     7501 ttccatacgt tcaacgccct ctatgattcg aataagcagc tagtttttac ttgtgatcga
     7561 ccagccgcag aattgaagga cttctctgaa cgcttaaaga gcagattcat gatgggactc
     7621 aagaccgacc taactactcc aagtttcgaa acacgagccg ctattgtaag aaaaaagctc
     7681 gaactctata atcgcgactt cagcgaagat gtcataaatc tcatagcaaa gagcatagaa
     7741 accaatgtgc gcgatctaga ctcatgtata aaccagatca tcgcttatgc agatctaata
     7801 aaggtagaac caacattcga aatagcacaa aacgtaataa agcagctcac aaattcattt
     7861 atgcctcctg ctatcgacgt atcttcgata ataaagactg tggccgatta ctataaactc
     7921 tcgatctccg acctaaaagg aaagaagcga agcaagaata ttgcgcttgc tcgacagata
     7981 gcaatgtaca taatacgaga aacaacagat tactccacaa ccgagatcgg ttctgaattc
     8041 aatggtagag atcatacgac agtcatgcat tcatgtcaaa aaatcgaaga tcttgttaaa
     8101 ttcgacgctt ctttcgcatc tgtggtaaat cgtcttatca aggaatgcaa agaaaaatcg
     8161 tcctcatagg attttatctt gttaaaaacc tgtggaaaac ttagcctatt ttgtggaaaa
     8221 atttttgatc tgtggaaaaa gtggatttct tgcagtaatt taccacaagt tatacacata
     8281 cctaactcca tatttataag ataattaagt aagttttcaa catatcaact gtgcttacta
     8341 ctattattac tatttaatat ataaatagaa taattaataa tagtccagaa atatttgtgg
     8401 ataattacct ttttaaatga tactgtttat ttttattttg tgagctgcta atataaaaca
     8461 tcttgatgga ggataagcca aatgagattt gtatgcgata gagatacttt tgcaaaagag
     8521 atagcaatcg cacaggacat tatcgcttcg aaaaatgcat tttcaattat gtccaatgtc
     8581 tatcttgaag tcgtggattc tagattgatc ataaaggcaa cagacgccaa ggttggcttc
     8641 gagacggaga ttcctatttc aaatggcgag cctggagcgc ttacggtatt ctgcgacaag
     8701 ttaatggcta ttaccaattc aattcccgaa ggagacatga ttctagaaca gcatgattcg
     8761 ctaattgaaa ttaaaacgct ttcaaggaat gtgcgctttc aacttaagac aatagctggc
     8821 gataaatatc cagttattcc agatatagaa gagaatcagt attttccgct gaaggctcgt
     8881 gacttacgta aaatgataag tcaaacaatt ttttctgttt ctaatgatga gacaaggttc
     8941 tttatgaatg gtgtcttcat cgaaaagctt cctgaaaacg tattagttat ggtagcgaca
     9001 gatggccgta gacttgccta tatatctata gaagaaggta atattccaga ttttcgccct
     9061 gcgataattc cacccaaagt gctgtctgta attctaaagc gcgcttctga cgaaggtcaa
     9121 atatacatgg ctataagcga gaagaatgtc ttcttccgtt ttggaaatta cgagttgaca
     9181 agctatctca tagaagggag atttccgaat taccagaaag taattccgca aaatcaacgt
     9241 caaaagttca ccgtgtcaaa agatgaaatg ctatcagcgc ttcgacgtgt ttcgcttttt
     9301 gtcgagagat ccaataaggt tatttttgat atttctagaa aaggcctggt tattaaatcc
     9361 gaagaaacag agctcggcgc ggcgcgagaa gagctagctt gtgaatatga tggcgatgac
     9421 gtggtaattt ttctgaactt aaggtatttg gaggatccac ttaaagttat ggacagcgaa
     9481 aatatcacta tcgagttcag cgatccaact agagcaatca ctctgaggcc cgagcctgcc
     9541 tcgaactatt tccatgtcat gatgcctata cagcagccat aaatggcatt cgagcatata
     9601 cgaatcgccc aattcagaaa cattgaagaa gcagaactgg agttgggcag cggtaatatc
     9661 tttcttcttg gagaaaatgg tcaaggaaaa acgaacttcc ttgaagcctt gtattgtctg
     9721 agttatggta attccttccg gactcatcaa gaccgagagc taataatgca tggaagatcg
     9781 gattttcttc ttcgagctga atggagcgag ccaggctaca tagtacgcga tcaaatatcg
     9841 tttagatatc taccagaagg ccgtaaagaa atattgctca acgaaaagca gctgtctgac
     9901 aggaaagagc ttgtcgccca caatccttct gttgtgttct gccatgaaga tttggctttt
     9961 gcctcaggag cgcctgagga gcggcgattc tttttcgatc aatgcgccgg aatgctctgg
    10021 ctcgaatata tcgatttatt gcgttctttt agacgattgc tgaaacatcg aaacatggca
    10081 ttgaaaaccg atacctttag tgtcctagat acgctcgacg aacagcttgc gcgctatggc
    10141 tctgagctca tgttataccg taagagactt tcgcatcttt ttaaggatca ttttccagaa
    10201 agatatgagc aagtctctca acttgggaca gaggtttcga ttcagtacag accaaactgg
    10261 ccagaggaag catctttgtc cgagattgta gaaatcttag cgcaaaggcg acagagagat
    10321 atcgaagtgg gcaccacttt atcggggcca catcgtgaca gatgggtatt taccatggaa
    10381 ggtcgggatt tttctgcttt tgcttctacg ggtcaactaa ggctggcctc tcttgttttg
    10441 cgtgtagtac aagtctacct tttctctgaa ctgtcccaaa gcgcagaaag cgcagaaccg
    10501 cgatttcccg tacttttgct cgatgatgtg cttctggagc tcgatgtggc aaagcggcga
    10561 aggttttttt cactcctgcc ggcaaaagat tcgggcgcac aatcggtttt cacctttctg
    10621 cccgaagaac catggcgaga atatgcaaat cggtctacaa tagtttacaa ggttagccat
    10681 ggacgattct cgcgtgaaga aagcatctga gattctttct cgttttttcg acgacaatat
    10741 gttcgaagct gctacgcaat tcgcggcttt tcgatcgaca tggaaacaga tcgttggaga
    10801 gcgccttgct gatcattcca agcctaaagg tataatgcat cactgtctta ttgtctccac
    10861 cgaacattca ggatggatcc agcttctaca gctgaaccag gagaagatat tggcgcgaat
    10921 atcgaagaac tatcctgagt tggatataac tgggcttgct tttacgatag aagataataa
    10981 agaggcttct tcgacgacag acagagccta agtagtcgaa aggcggttga ccaaaggctc
    11041 ttttgcagtc tatactccag cgcttgatta tgcaatttcc cggttgcagt gagagatctt
    11101 gggaagggca aatgcagccg tttacgcaag gagaataaga ccatgaaacg cacctatcag
    11161 ccgagccgag ttaagcgcaa tagaaaattc ggttttcgtg ccagaatgaa gactaaaagc
    11221 ggccgtcaga ttcttagcag aagaagagca aaaggtagga taaagctctc tgtctcagat
    11281 gagaagaaga aatactgaaa atacccagag cggaattcag gaggaacggc aggatcggag
    11341 ctttccgaag agccttcggg tgaggaaaac caaggaaatc gatctaattt ttgagaaagg
    11401 agcgcggttt tcctgtaaag gcatgagaat gcatgtcctt cttaaggaag ggcaggaaag
    11461 ccgagtggca tttattgcag ggcgtgcttt ttctggcgca gtaacaagaa atagagcaaa
    11521 aaggttagcc cgagaagctt ggcgacatat tcgtaattct gtcaagccgg ggtttgatat
    11581 cgtattcgtg ctctatcctg ggcttgaaac atttgtagaa ttttacagcg ctatgcagta
    11641 tctgcttcgc aaggcggatt tactgttatg aatattgtca aacaaatatt ctcatttcct
    11701 ttattgatag caattcgctt ataccagctc tttatttcac ctctttttca accatcgtgc
    11761 cgattttacc ctacttgttc ttcttattct tacgaggcga tccataaata tggaccccta
    11821 aaagggcttt tgctagcttt taaaagaata attcgctgtc atccaggaaa tccaggaggc
    11881 tatgatccag taccatgaat gaaattaatg aacagcaaac cacctcgaaa gaagaacgca
    11941 aacgcatgat tttggccgtg gtcctatcca cggtaatagt aagcgttggg tttattgtgc
    12001 agaatatact tttccctccc gccactcaac agcaaacaac gcagagttct gaacaaacag
    12061 gagcgaatgg gcaagcaata ccgcaagagc cgagctcagc aactcctaca aaagcactac
    12121 cttctattat tccaatatct accgagaacg ctcctatagc caacatgata cctgttccaa
    12181 ccacagaaga gcgttatatt gtcgagaccg atgttgttcg cgcgacattt accaacgccg
    12241 gtggagaact tgtctcgcta accctgaaaa agcacaaaga tttgagggat tcaaggggcg
    12301 gagtagacat ggtcgtagct ggctctcagg gtgcagatgg gctttctgtt tcctttggcc
    12361 aatcttccac accaatgcgc gagcttatga acgcccagta tctagatgag agcaagaaca
    12421 caattgcatt ctcgaggatg ttcgaggcaa agatggcagg aagcgacgaa aaagtcccct
    12481 ttatatttaa gaaggttttt tcgttccgcg atggagaata tctatttgga cttgcgatta
    12541 gcatagaaca gccagaaggc aagcctatta ttctgggaac agacggagtt gcctatagaa
    12601 tcgaccttgg gccacagatt ggtcctcgat tcgatcagat gcctaagaat gctgattacc
    12661 gaaaatatat cgctgaaata gatggcaaga aaaagaatca gaaactcaag cctaacacct
    12721 cagacaccat tgcaccgaat gcaagctggg tggcactatc tggcaaatat tttacgttca
    12781 ttgcggttcc acaagcgccg ttatctgctt acgaaatagc agctagccag gatccactta
    12841 ttaaccagac caatagtctg ggacttttac gaagcacatt ttccggtgct tcggttaccg
    12901 atacttacta tttctatttt ggccctaaga cctcagcaga gttgggaaaa tatgagtatg
    12961 cggataagaa tggatttggt ctttccaact taaagctgga agacgccatg gagggctcag
    13021 gaattctagg atggctggaa aacatattga aattcttgct caatgttttt tatcgcctaa
    13081 ttcctaatta tggtgtagcc attattctcg ttacggtgct gataaagctg ttattctacc
    13141 cccttaccaa gaaagggtcg ctcgctacgg cgagaatgtc ggagcttcag cccaagatgc
    13201 aggaattgca ggccaaatat aagaacaatc cacagaagct caatcaagag atggcagagc
    13261 tatacaagcg agaacattac aatccaatgt ctggctgtct gccaatgtta atccaattcc
    13321 ccttattcat tgcaatgtac aatctattca acaaccactt cgagctcagg ggagcaatgt
    13381 tcataccagg ctggataaat gatctctctt tgccagaaag cctcgtaaac tttggcaatt
    13441 ttaaaatacc tcttcttgga tggaacgatt tgagggcatt gcccatcata tacctattta
    13501 gccaattatt gtatggcaag tttacacagg ctcctcaaag cacccaaagc aacaatccac
    13561 aggctaatca gatgaagttc atgatgtatg gcatgcctat tatgttcttt ttcattcttt
    13621 acgatgtgcc tgccgggctt ctgatctatt ggattacaaa caatatcctg acgattgctc
    13681 aacaggtcct aatcaacaac ctgatgaaaa aacgcaaaat agctagtgcc gaaataactg
    13741 ctggaggaat gccagctgtg ggagcagcta gcgctggtcc gagattagcg accggcaaga
    13801 gctcggcgat agatacaaaa gctaagaccg ttggcagaga acccacaaaa gaggcttttt
    13861 cggaaagagt gacaaaatgg ctcgaaaagc aggccgccga ggccgaaaaa gcaagcggtt
    13921 ctgcatcttc aagaaagaaa ggcgaaggcc gcagctatag ttctgacaga tcatcagtaa
    13981 cacggaaaaa taaggccaag aaatagatca ttgaatcttt caatcaggag cgatccaaat
    14041 gatatatgag tttgaaggga aaaccgagcg cgaggctatg gaactagcca cccaggagct
    14101 cggcctggat acaagttcat tcgatgtaga gatcatcgaa aaccaaagtg gtggcttgtt
    14161 caagaaagga aaggtgcgca tccgcgtcca taccaaagat tcttcctttg cggaaaatcg
    14221 ggcaagagaa tccaatgagt acaatgaatt cgaggaaaaa caagaagccc gagaagcccg
    14281 agaaacccga gaagcccgag aagagaggca tattgagccc ctccctatgg atgatttcga
    14341 gagcaaaatt gtagaatgga ctactgaggt tatcaacttg atgggctata gcgctcaagt
    14401 ctccgtggcc tttagggaac ccaataagct tggcttgcgt atagacaccg actctgcctc
    14461 aattctgata ggtaaaaaag gtaggaacat cgatgcgctg caacttctgg caaatgtcta
    14521 tgcgggcaat cttggccata gcgacaccaa gatcgtgctc gattccgaaa attaccgctt
    14581 gcgacgcgaa gaagcattgg tcagaattgc gtacaaaacc gcagaagaag tacgcagcac
    14641 agggcactcc atcctgctag agcctatgaa tcctttcgag cgtcgaatta tccatacgac
    14701 cctcaatgat ataattgata tagagacaaa gagcgaggga gatggcctta taaagcaggt
    14761 acgcgtaatg ctgaagggaa gaaatcagta agcattttgt ggacctagaa tgataatcgg
    14821 gaagaaggcg ccgcaatacc attaagcggc gctttttaat gcccgctttc ttatgctcag
    14881 atgtcttgtt tcgatatttc gctgtgacat ttctccagat ctttcgttca aatattcaat
    14941 aaatttttat ctttttatta tacaacgaat acttatactc tttttacctt gaccacaatc
    15001 gaaaacaggt gctaatatag ctcgattttg ttataatttt ctatcatttt acattgaatg
    15061 cgaagaggat tggattcttc gcgaggtcaa agtaaatctt caaaggagac ctttctacta
    15121 tgtcaatcaa gacattcaga ggcggggcac atccgtccga gagaaaggat cgctcgcagc
    15181 atgcaccaat cgaaaaggtg agttctgtgc cgcaggtagt gatccctgtc aatcagcatt
    15241 ttggtgcccc aattcaacct ctaatcaagg tcggagattt tgtgaggctt gggcagaaaa
    15301 tagccgatgc agaaggtcgc atgaccgtac ctatccatgc atcgatagcc ggcgtggtca
    15361 agaaaattga gccgcgaatg caatcgaata acatagaagg tctatgtatc gtaattgagc
    15421 caaaggatac accggaagga gaatccgata gagaattcat gcctcccctc gatccattcg
    15481 cctgcagaaa ggaagaggct ctcttgcgca ttcgtgaagc agggatcatc ggtatgggag
    15541 gagcgggttt cccggctcat gtcaagctcg ctcccacaaa gccaatttct ttggtaattg
    15601 ccaatgcggc agagtgcgaa ccttacctga caattgacga aaggactctc cttgagatgc
    15661 cagataagat cgtcgatggt ctttcgatag tgatgaaaat cataaatgca tccgaaggta
    15721 tcattgctat agaggaaaat aagaagtaca caattccgct tgttcaaaag gccattgaag
    15781 agaatccgca agttaaggcc ggcatgaaga tcggtattca agtactcaag acaaaatacc
    15841 cgcagggcgg cgaaaaaatg cttatcacgg ctatcacagg cagacaagta ccatccggag
    15901 gtctgcctat ggatgtgggc tgtgtcgtat cgaatgtggg aacgctatgt gcaattgccg
    15961 atgcattcag agaaggaaag ccacttatag agcgtggatt tacggtgacc ggaggagctt
    16021 gcaagactcc aaagaacctt attgcgcctg ttgggaccgt ggtggccgac cttgccaaat
    16081 ccggaataat cgatatcgat gaagaatcgc tttccaaagt tatctttggc ggaccaatga
    16141 tgggagtggc tgtaccgagc acagaaattc cagtgcaaaa gaacacttca ggcgttcttc
    16201 taatgagtgt tgaagaggct ttgcggtttg aagagaagac ctgtatccgc tgtagtcgct
    16261 gtattcgagc ttgcccgatg aagattagcc ctgctttgct tgctattgca atcgagagcg
    16321 gcaactatgg cgaagctgaa aagatcggcc ttcaggattg catagaatgt ggcgcctgct
    16381 cttttgtgtg tccagcgcat cgttatcttg tccagcgctt cagggttggg aagcaagtac
    16441 ttcgcgcgct gaaacagaag gaggcacaga atgtcagata agaaaatcat actatcctcc
    16501 agcccgcaca cattctcgcc agtcgacact ccgcgcatta tgctttctgt cgtaatagca
    16561 ttggtgccag cagcggccta tggcatatat ctctatggat tagctgccct tggagtcatt
    16621 cttacttcga ttacagcggc tgtgctatcg gaattcttat ttagaaagct tattaaagcg
    16681 agcacaacgg ttggcgattt ttcagcagtt gtcagcgggc ttctgcttgc gctagttctg
    16741 cctccagcca ctcctctctg gatggtggca cttggtgctg tctttgccat tgtagtggca
    16801 aaggagttct ttggtgggct gggtgcgaat ccattcaatc ctgcactgat tggccgtgct
    16861 tttctcctta tgagcttccc cgcggcgatt acaagctgga acatgccaaa aggccttgca
    16921 ttgccagccg atgcggttag tacggccaca cctctaaatc taatcaagca gggcaactca
    16981 ttggccgatg ttgccaaata tttcggcgca agcgatatcg gggcatttta tcgtcagcta
    17041 ttcataggat accgcccggg taccattgga gagagctcga ttttgcttgt gcttctgggt
    17101 gggctttttc ttcttgccat cggggtcatt cagtggatag ttccagtatc nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgggtgggct ttttcttctt gccatcgggg
    17281 tcattcagtg gatagttcca gtatcggtgc tagcatctac atttgttttc tcttggcttt
    17341 tggggatgga tccagtgttt agtttgttga ctggaggtat agcatttggc gcttttttta
    17401 tggcgacaga ctatgctaca aggccactga cagaaaaagg gcaggctctc tatggtattg
    17461 gtattggttt gattgctgtt cttataagaa aattcggtgg atatccagag ggagtgacct
    17521 atgcgatatt gataatgaat gtcttgacac cttttctcaa taagctccga acgaaaaaat
    17581 atggctttct gcctcctccc aagcctgtta agccgtccaa ggaggctaca aaatgaaaaa
    17641 gactaagctt ggcttaattc ttatgctgta tgcctcgatt gcttgcgcaa gtcttgcggt
    17701 tgtctatgcc tttaccaagg acagcatcga aagacaaagc gaaattcaac tgcaggcttc
    17761 tttgaaagat atctttccgc aggcgactgc cttcgatgtt gtcgagaatc ttacaagccc
    17821 ggagccaaat attaaatttg aaacagcata tgaagtaaaa agtgacaatg ttccgctggg
    17881 attggcaata aaggtttcgg gcccaagtta tggagggaat gcgacccttc tcgttggcat
    17941 tgatttgcat cgcagcatag cgggagtaag gataatggcg ctcaatgata cgccaggtct
    18001 tggaatgaat gcatctaatc cgacctatta tgtagataaa gccaaaaagg tgacattctt
    18061 aggtcaattc gaaggaaagt ttcttacgga tccctttgag gtgaagaaag atgttgcagc
    18121 gattacggca gcgaccataa catcgaagtc tctcaccaag ctcatcaaga ccgcaggcga
    18181 tgcagcaatc gcctatatgg atcagaaagt gatgatggca tcgatggaag acgaaaagcc
    18241 aactggcgag acatctatag gagcatctcc tgcggtttcc acaggatcgc ccataggagc
    18301 accgggggca tcggcaggaa cagccaaaga gagcgccagg ccccaagcag gaggcaaatg
    18361 agaatgaaaa agctgtattc ggttttcaaa caagggatca tttttgataa tcctttattg
    18421 atgcttatga ttggactctg ttctgcaatt gcggtaacga ctaatgtctc gaatggcatt
    18481 ggtatgggac tggcaatgac ttttgtcctt ctgttttcgg aaataataat ctctatattc
    18541 cgcaacctta ttccttcgat gatccgaatg cccgtgttta tcatagtcat tgctgcgttt
    18601 actacgatcg tcgacttgat gatgaaggcc tactttcccg acctttcgaa gagcatgggt
    18661 gttttcatcc cattgatcgt agtcaactgc ataataatgg ggcgcgtaga ggcctttgcc
    18721 tctaagcaaa atctgccatc ggcaatcgcc gatgcgctgg gaatgggact cggctatact
    18781 tgggttctta tcggtcttgc tgcaataaga gagttgcttg gaaatgggac ttttgcgggg
    18841 attcaagtca tgcctgcctc ctatgaaccg attctcttct ttattttgcc gcctggggga
    18901 ttctttgtct tcagtctgtt tatttctttc aatatctggc tcaagaagaa aatggaggca
    18961 tctgcgcgca aaaaagaatt agtttctaag aaggaggccg cataatggat cttggcaaga
    19021 tttttcttct gtcgatgctg gtaaacaata tcgtggtaat gcgctttatc gcgctttgtt
    19081 cgtatatagg gatgacaagc gatctgggcc aatcgatggg catgggctat gccgtaacct
    19141 ttgtaaccgt gctaacgacg gcggcaacat ggcctatcta ccattacatt cttgtgccac
    19201 tcaatttgac cttccttcag attctggtat tcattcttgt cattgcaagc ttggtgcagc
    19261 tcgtcgaatt ctatctcaag aagaatattc ctggtttgta ctcggcgatg ggcatctatt
    19321 tgccccttat aacaacaaac tgcgctattt ctgcggtgac tttcgagaat atctcgaata
    19381 actacaactt tattcagtcg attgtgtacg cgattggcgt atcgcttggt tatcttttgg
    19441 ctatgattct ccttgctggc ataagggatc gaatgaaaac gagccctgtg ccgaagtttt
    19501 tacaaggaac gccgatactt tttatggcca catccttgat tggtatagca tttatgggtt
    19561 tcacagggct tataaaataa gaggatatcg agatgcttat tacaattctt ttgacaatag
    19621 gtttttctgc agcgctcgct tttatccttg gcgtggcctt gggcttcttc aatgagaaat
    19681 tcaagatcga acgagatcca aagatcagcg aagtaagaca agccttaccc ggagcaaact
    19741 gcggagcttg tggctatcct ggctgcgatg gttattcgga ggctgtggcg accggccgcg
    19801 ctcccacaac taagtgctct gtaggcggat cggctacagc cgaaatgatt tcgaagatta
    19861 tgggtataga ggcagggact gtcgaagaga aggtagcggt tctgctatgt cagggctcaa
    19921 tcgataaaac agcaaagaaa ggcgaataca gcggtattcc cacctgtaga gccgcaaagc
    19981 tatcgactgg cggcattaaa gcctgtgcat ggggatgcca gggatttggc gactgtgtgc
    20041 tcgtgtgcaa gttcgatgcc atcgaaatgg gagaagacgg tcttcctcat atcgattatg
    20101 acaactgtac cggctgcggc ctctgcgtag aggagtgtcc acagaaaata ttgacgctta
    20161 taccacttac aaaggctggt tccatcgtcc tatgttcgaa taggacagtg ctcaagcaga
    20221 ccgtactcaa gaactgcaag gtcggatgta tcaagtgcga tctatgtgtt cgttcttgtc
    20281 cggaaggagc aatttcgatg ttgaatggca ttccggtaac agattatgcc aagtgcaatt
    20341 catgcggtat atgtgtccag aagtgtccta caaaatgcta caagctgcta gagacagagg
    20401 tgtttggtac aaagaagctg attcctgtca tggaagaagc ttgaagatag cgcagcaaat
    20461 aatataggct gtccaataag ctgggcagcc ttttttattt tccataacga ctagaagatc
    20521 aaaatcaatc aataatgcac attgcgcaag cgaagaccaa tcgaaatact aaggcaagat
    20581 tgaagatcgg accgaattga ttccacctgg ccttgcttag gaacataata atcatatctg
    20641 agttcaatgt agctaccaag tcgatgagcg cctgtagaat aaaagaaaga cgaccacctt
    20701 agcccccctg tagctaagaa gaaatctcct ggggcataca gtctgtcatc cagcgctggc
    20761 gatccgaatg tgaatgctgg accagcgaac acagatatac cagaaatctg accaagatcg
    20821 agctcgatag gaactctcac cacgctaaga gtacgatccc attgtgcgcc tgcccgcagg
    20881 cccgcattaa aattctttgc gatgggaagc gaaatctccg tccagcccga gactccgcgt
    20941 acaaaggcta gaagatcacc ctgtagaagt gtatccaatt gggcaaaact aattgcgcct
    21001 ccccagtctg aagcaaaacc agaaaaacca gaagcaggaa gagcgcgtcc cgcaaacaag
    21061 aaccgctgtt gccagttttt ttctcttagt gaactgtgaa tgactcctgt tttagctcct
    21121 gtgctcgtgg catggataaa gatgtcatca cctagatata ttcccacatg gtcggctttc
    21181 gaaagagtcg aggctgctag actcgaaagg cctatattaa aatatcctaa acccgagggg
    21241 acggaggttg agacattagc gcttgagctt ttagaaaatg agtttgtttt tgcttctagg
    21301 ctaaagaaca cgaggtcgcc cggttcgagt tcattcttag ggatgattaa gacccatttg
    21361 acaagtgcat ctacagagcg cggaatcttt actccagttg cttttagata agatatatat
    21421 actaagccag aacaatcgat gcctgacttg gaagaaccac catagcgata ttgagtacca
    21481 agataggatt ccgcagtgcg cagaacagcc tgacgtgcgc ctgaacttac tagccccgct
    21541 aaaggtggaa cggagacata agcaaaagca gattgcaaag cagggacaga ggaaagaaag
    21601 aagagaatag ccgcccaacg tatcgctgct tgcaataatg cagcttgtcg ctttctaaaa
    21661 agcgctacca taataatatt atcggaagag actatattgg catgaattat ttattatttt
    21721 catatctaat cgcccagcgc cgaagtttgc tggatgcacg ataaacttgt gcaaagttat
    21781 ttaggcagct atttttcgag gggtgcacga tttttcgagc gcacgcttga ccaatcaaga
    21841 aatgatcatt agactttatt tataagccag gtcggcagaa gttttccaag cgtcctggtc
    21901 ccgccgattt atccgaat
//
LOCUS       DAOSOB010000030        21352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_2471_length_21352_cov_82.573696, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000030 DAOSOB010000000
VERSION     DAOSOB010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 21352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21352
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2471_length_21352_cov_82.573696"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1069)
                     /locus_tag="PLS88_04680"
     CDS             complement(266..1069)
                     /locus_tag="PLS88_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOU06674.1"
                     /translation="MIKPLEIHYEVRGYDCGYGGPLKPFALANFFQETAGAHATVLGI
                     GMEDMWARGLTWMLSRIDIRIEEMPGAGQRVIARTWPVGAKKLYAQRCLELLDESGFR
                     YVGAMYEYIIVDVQTRRVVRPERTLPLDLTTNYPWPFDDLSPGIEESQFGALEEFSEA
                     FTLEARARHIDQNGHVNNAHFINWLCDAVPRADEETICRIKVDFVHEILKGEKVSAWI
                     KPAQAVSNPVYRKALKQAEKVKAPHPTWLSALTCDGILAARGMIELDSL"
     gene            1322..3739
                     /locus_tag="PLS88_04685"
     CDS             1322..3739
                     /locus_tag="PLS88_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOU06675.1"
                     /translation="MCGIVSLVYKDENPSMGKEAEALLKRLEYRGYDSTGAAFIDADK
                     RITLRKKVGAPSKVCPLLQIDSFSGQRFIGQVRWATYGGVTDVNSQPHHVHCKMELVG
                     AHNGNISNTDTLKTWLTARGHAVISDNDGEIIVHLIEEHYSAAQSLASSDLAYLRKAY
                     ANAGLAEGVPDGVLRMIEAIRRAQALAEGSYAAAIADPQLPGVFAIKSGSSLYAGVGS
                     DSFGNFIVLSSDLTSVLSKTRTLIPLSEGEGIWFTENKYLVFNLQGQPFFSHPRLKRS
                     KLSIKDTQLSPEYQHYMEQEISSSPNNIIQILRYYFRDPATEPLSELFEEHKDDCKET
                     ADKISSLSERFGNESLLAGMKEVFESPAWNEIEARVQNTGAAHFVGQDFISDETELLK
                     ELAALNPPTRGKLLLMDKVIVWRKRRAVLRYTGELKNAIREATQSGGRIYFVASGTSY
                     HAALIAAYFFNSIAHIPVYPCNPGMFRSMYFNCLGKNDLIIGISQSGETKDLVDIFQD
                     VKKANNSVRLVSIVNNENSRIPQELSDFYLPLLCGPEIAVAATKSFTSQIAVLYLLAA
                     SFSMPEHRIRENLLKAKDLMTETIETRMGDIESVAAHLFAASSIHILGTGLIGLAKEG
                     ALKIREVVLNHTEGYDAAEFKHGPNTILGRNTILSLADLEKFVGAEPLTAINNDLFEK
                     LTHNYPLIFICPPEERDRRITISQIHTHKIRGADIVLIAEPSQELALAVEGAPMGIEK
                     YWSKYIPLPQSGDPCLFVFSTTIALQYLAYRMSVRKMQWLDSLGIQNHGVHPDAPKNV
                     SKSITVD"
     gene            complement(3777..4898)
                     /locus_tag="PLS88_04690"
     CDS             complement(3777..4898)
                     /locus_tag="PLS88_04690"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HOU06676.1"
                     /translation="MLYWTVERTGNPRFPYRIAISDENGDFFAVRSQDAWPGPKGNIF
                     CIRDGSSPAERDLFMEIERIPVLSFERFGRSLKIILDRPTKKRCEFLILEKAYRNREG
                     SYEQIFFKTDSAQKEHKSRSRVALRPGLTKLELTVAIDSAERYPWKFPKAKVEQRRLP
                     AGDYALMHNETILAVVERKTYENLLTDFDSIAILHRSLDELSGLPNAAMVIEAHYGDF
                     MNPKRLEGRWPAAHAYRVISELQAMHPHLPIIFAGTRKEANLWTYAFFRAIAWKHERE
                     RLDEDSILLKEPSAFLGAISSNISSDISPRLDEKVYAVLNAKESGLTLRELALMFEDV
                     DAGSLRHCLERLRRRGRVQCEGRGISARWYFSPPDHMQE"
     gene            5071..>6114
                     /locus_tag="PLS88_04695"
     CDS             5071..>6114
                     /locus_tag="PLS88_04695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU06677.1"
                     /translation="MLFFATIINSMGMFVFPFLTLYLTGRLGMSQEKAGFFIMMLYLV
                     YGPASLIGGKLADSFGRKKLMVIAQVLSGLCYIPCGFPTVGSNIRWFLLASVFFDGLT
                     DPARSAMMTDLTSSENRRPAFSLIYLGHNLGFAVGMLLAGFLFENYTSWLFWGNAIAI
                     FAAITLVGLKVPETKPSRGQIEKSFGTGRQDEAHRGNIIQALLSRPNLIIFTLLTGLY
                     GFVYAQHRFILPLQTKEFFGARGSVIYGTLMTLNAVMVILLSTPIMTITQRWKPINAV
                     AFSGFLFALGFGFIGFRPSVMLMYVTTAIWTLGEIVNATNEGTYVANNTPISHRARFQ
                     AFLPLLGGIGWTIS"
     assembly_gap    6115..6214
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6215..6404
                     /locus_tag="PLS88_04700"
     CDS             <6215..6404
                     /locus_tag="PLS88_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06678.1"
                     /translation="ARFQAFLPLLGGIGWTISPPIVGSFTDHFGLRMAWPILGAIAAL
                     AGLSIFYLGLMEKRANKK"
     gene            complement(6522..6593)
                     /locus_tag="PLS88_04705"
     tRNA            complement(6522..6593)
                     /locus_tag="PLS88_04705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6559..6561),aa:Val,seq:cac)
     gene            6637..7320
                     /locus_tag="PLS88_04710"
     CDS             6637..7320
                     /locus_tag="PLS88_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HOU06679.1"
                     /translation="MSRMAKGTSKLSKSRKEASPNSTKPTLRRLNIRFQSPADELRIE
                     LTRKSLHLLILFCVPLLDISPMLPVFALIVGMVIYSISEALRQRGIMVPVISSITERA
                     ARLRDNNHFVWGPITLALGALFSLLIFDPLQAKVAILALGLGDSFSSLVGKSFGRIPM
                     PFSGGKSVEGSVLCFAIVLLAVYLLTGRFMPSMIIALATTFAEFWPTKDWDNILIPLA
                     AGIVASLVL"
     gene            complement(7416..8186)
                     /locus_tag="PLS88_04715"
     CDS             complement(7416..8186)
                     /locus_tag="PLS88_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06680.1"
                     /translation="MLVSSVVLAVRIGTDRRLPFFLADLHLYTEYFFLGSPMGIQLAG
                     ALECCFLVLVATAMLIVVLFTFQKTVSAEIHLIALWIFLVEAEALRLPMLIMAQRSPS
                     ISSLISLTRLVYGARFSGLISLFISGLYAVGIGKEKPQTGYLFALLLGSMFAMLLPIS
                     IDRFQSSFMLQAGYSELTMIVTASLIVLNAIDYFVAARLKEDRSYAFVGIALTTAAAS
                     WVWLWDTRVFEWGLIVVIAFVLCVFASIKILHHVYIWK"
     gene            8334..9791
                     /gene="proS"
                     /locus_tag="PLS88_04720"
     CDS             8334..9791
                     /gene="proS"
                     /locus_tag="PLS88_04720"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOU06681.1"
                     /translation="MAEKITPREIDYSQWYIDIVLNAKLADYSPVKGSMVIRPRGYAI
                     WEKIRDEFDRRFKETGHENAYFPLLIPMSFLKKEAEHVEGFAPELAVVTHGGGEELEE
                     PYCIRPTSETIIWAMYRNWIQSWRDLPLLINQWANVIRWEKRTRLFLRTSEFLWQEGH
                     TAHETEKEAREETLRILEIYRDVVERCLAVPVVAGMKSESEKFAGAVETYTIEAMMQD
                     KKALQAGTSHFLGQNFARAFDVQFQNRQGQLDYVWATSWGVSTRLIGAVIMTHSDDKG
                     LVLPPTLSPEDAVIIPIYRNDSKAAVIAYAKKLEADLKTAGFKVVLDIDDSSSPGWKF
                     TEWELRGTPVRIEIGPRDIKSNQIVMVRRDTGEKLTALSTEAASKLKELHNLIQSSLF
                     ERARSFREKNTEPISDLGGLIEFFGTETAGTEGAPGGFAEALWCGSAACEASIKEKTK
                     ATIRCLPLGKQDHIEGTCAICGDPAKYQAIFARNY"
     gene            complement(9845..10231)
                     /locus_tag="PLS88_04725"
     CDS             complement(9845..10231)
                     /locus_tag="PLS88_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06682.1"
                     /translation="MSSTEKNKIDLKADLKDAAMRKAPAFPDFAQVYKKAEEQSRKRN
                     KFLISMALAITALVVSFFVGSFWKSRSSFNVALIDSWSTTPAPIAIAISGSEKEDDQI
                     VLTSSKNATVNLYIQGLWESTSAGGL"
     gene            complement(10218..10895)
                     /locus_tag="PLS88_04730"
     CDS             complement(10218..10895)
                     /locus_tag="PLS88_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU06683.1"
                     /translation="MKSENRMAWTMSPAKKDRAQIGQNKAEASSLAFSSDEETLDIEL
                     ATRAAKGDKGAFERLIWRWWDRIRGYCASFVAFDEELSEEAVQESLIRIYKALPKWRK
                     ESSLGGYIYGICRTSCADVLRVNARNHARNFSVEEFESLFIENPHGLVEEDMLRKEAS
                     QMLELVMKKLEPEDRSMLYLHEVEGIELSELGSMYNLPLGTVKSRLFRARAKLSEMLK
                     EMGYELH"
     gene            complement(10923..11477)
                     /locus_tag="PLS88_04735"
     CDS             complement(10923..11477)
                     /locus_tag="PLS88_04735"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOU06684.1"
                     /translation="MKANRARLSVLLILITSIAFLGILPVAAQAGSGKGMGNQGVMGP
                     IVVREWFRSLNLTNEDLDKLEKILESREFELIKAQNEIRILQAQIANILVDPDPDLKA
                     IEEAISKSLQYEKTVRMIQIERQVAIRKVVGEDRWQTILLLVHEARMSEKTGKFANSF
                     STKGLSVEESNRYSHLLAILRRIM"
     gene            complement(11658..12200)
                     /locus_tag="PLS88_04740"
     CDS             complement(11658..12200)
                     /locus_tag="PLS88_04740"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOU06685.1"
                     /translation="MRTSRKAIKFLMVGVMLVAATAGAFAQGFGRMPSPRARTPGYGY
                     QSMPPVASPMVDPYGNRFGDASKGLANPKQSFFGGMRSMGAGSYGAFHYFQYQKLSDE
                     NKAKVDKIIEDNASKILPLRNELRAEKLNLDNLLWSPNPDKAKIDETIAKIADIQKQI
                     QEIRADSILEINKIFQSANS"
     gene            12693..15728
                     /locus_tag="PLS88_04745"
     CDS             12693..15728
                     /locus_tag="PLS88_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU06686.1"
                     /translation="MPFVLQKESSVPELDSLFRLYRHEPTGARLLSVINKDENKVFGI
                     SFRTPPERNNGVVHIIEHSTLCGSRKYPVKEPFVELMKGSLNTFLNAITFEDKTCYPV
                     ASTNLKDFYNLIDVYLDAVFYPNLTEYTFMQEGWHYEVDPLTKELSYKGVVFNEMKGA
                     YSDPDSLHDHLCKCSLFPDTPYGLDSGGDPLEIPTLSYEEFIAYYKKHYHPSNSFIYF
                     YGDDDPEKRLSFMDKWLSPFETASIDSLPAIQQTFSEPRLIERSYQINSKEEAKAYTA
                     LNWMLYEHGDLLLSMQALVLFHILTGTPASPLRKALIESGLGEDIAGFGFYEEMRQTA
                     FSIGLKGVKPNNVTKVEELIFSTLERLARDGIDPDTIAASLNTIEFALRERNTGSFPR
                     GLAIMLDALNEWLYDLDPIAALAFAEPLEQTKKAVKENPRLFSELIDSLILKNRHRTT
                     VRFLPSSSSNHEREDAEKAMLALARASLDDEALKKIEDTAKTLKELQDKPDSPEALAT
                     IPVLSLDDIPTESPILPSKPLESATDKLEGSGIYGYYHELSTSGILYLDLGFDFEKLP
                     SELLPYVSLLGRLLIETGTEKYNFVQLIQRIGMHTGGIRSMAISTTHWTEHKPVPWFF
                     LRAKALPEKVGMLTDILLDILMSARLDDRERIRQIVLEEKAQAEAMLIPASARIVGLR
                     LRSRFNPAYWASEKLMGIERLFFLRKLLHRIEEDWPTVLSEIREVHRELIRRDNLLVN
                     ITSDRTILEASAESILRLAQSLPIQSTLAQSFSDKPSFELDSWINEASHACDPSISRT
                     SKHLENISLEDESVHSARPALETIELQTQVNSVGMILPLTGLDPIAGGALVATKYLDT
                     SYLWEQIRVIGGAYGGFSSLDLASALLMFLSYRDPNLDRTIEVYRKVGAFLESCDLPR
                     EEIQKSIIGTIGDVDAYQLPDAKGFNALMNILSGYTQETRQHIRDQILAADIEDFHSL
                     GKLLSEAAEHSMIAVMTSPEQAEKSLLTLPAPVVRLSVH"
     gene            complement(15761..17200)
                     /locus_tag="PLS88_04750"
     CDS             complement(15761..17200)
                     /locus_tag="PLS88_04750"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU06687.1"
                     /translation="MRHSSHNILRVFIVVMIIVGVATGACATTPYVRENAPTYMNTVL
                     SNGIPLYMKVQHANQVFHVSLVLNGASLVTAPEQAGWEKIALATMARSSKNYPYEKAA
                     SILDRRSSAITSAVQFEYSTFSLTTLDKYSDELLALWSDMLVNPSFDQSDFDKEKENA
                     LLALQAIEQNPWTMTQKIMNEAYFKGHPYGVNPEGTEESIAPMMVGDARGWYEKHFSA
                     DRIFLVAVGDFNPAELAYKLEKLLGDIPNLNLGPVPKPPNFPMGSSGSELALGSLITQ
                     AHDQSKGIIYLRGDFAAPAPGSDDYFATTLATRIFSDLLFAVVRDKYGAVYTPSAAIR
                     SHTANYGSIMIYKSDAPQKIKQYIDEAVALMANGRVVSVDPTRTDADGYMGLSDALDT
                     YKQLYINDYFEAVRTNAAIASLMIRSIKLTGNPADWLKDQNRIEILDASAVYNAFEKY
                     VHNGTFLWVAVGDQALLDKLDWKAFESMR"
     gene            complement(17211..18704)
                     /locus_tag="PLS88_04755"
     CDS             complement(17211..18704)
                     /locus_tag="PLS88_04755"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU06688.1"
                     /translation="MRHKNSFFCIMLSLFFMLSTLDIVVAQQAEAVRNTTLENGLEVF
                     VVENHAVPLVTVCIAFRGGAMAHTPETAGLFHLYEHMMFNSNDKYPTKDAFSAALNRM
                     GTTNWNGATGIEYINYFISVPSEKLPDAIDFWAHAVMNPTLDARVLENEKQVVLNEIR
                     GYHSDPSQIASNGLESRMFKDFPWRKNIDGPESNIQIASVTALRDIQKTYYVPSNMAL
                     LIGGDTSLEEVVKLAKASFGSWARAPEPVLAEPPHGPIPDGIRLVTSEDQFYRGIAQV
                     EFRWRGPDVTRQTYDTYISDVLLFLLSSPSGPFKSEIMRKVYGLFDAEYIDFVYPTSR
                     DGGNYIFSTLMLVQRPTEEEPVLQRVENLRSAVLDEFALIAKDPCAYFGGDEALEEAK
                     TKLVDQNIYALESAGSFVTDTLTFWWSTAGTEYFFDYEENCRKVSWRDISSLIERYLA
                     GASTPSVATLVRLRTSTFNADPRMSAKISELGYALLTAQNAFWWQKQ"
     gene            complement(18743..19261)
                     /locus_tag="PLS88_04760"
     CDS             complement(18743..19261)
                     /locus_tag="PLS88_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOU06689.1"
                     /translation="MGKLYYSYQMIHKLAQSIAEQVKESGFSPDVIVAIGTGGFIPAR
                     ILRTYLGKPILAVGIAYYDLNDRPMDYPRKVQWIEEAEKQLAGKRILLVDEVDDSRVT
                     LEYCTKELLTYRPAALAVSVLHNKQKEKRGTFPPGVQHVFVGENLGDDWIVYPWDARD
                     IDEHDKNATLGH"
     gene            19439..19882
                     /locus_tag="PLS88_04765"
     CDS             19439..19882
                     /locus_tag="PLS88_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOU06690.1"
                     /translation="MNELDRFETFLREHGQKVTKPRRIILEAFLNTEGHLSTEDILRE
                     AKKHDPGIGQATVFRTIRLIEDAGLAREAVQEDGARTFEHLADHPHHDHLLCTECGKI
                     IEFLSPTIEKEQQKIFVKYGFRPIGHNMELLGLCPECQAKEKGAQ"
     gene            19884..20288
                     /locus_tag="PLS88_04770"
     CDS             19884..20288
                     /locus_tag="PLS88_04770"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOU06691.1"
                     /translation="MQLAELSPGQRFKVINVAVGGEIGKRLADMGLVSGAEGEVVRAA
                     FFRGPIQIRLGDYDLIMRRYEARLVDVEILPEAAPQMASKAPKKALATMYVDLRPRGR
                     RRSHGAYGEAKGTHGTHGKTHGAHDKDPSCLE"
     gene            20310..>21352
                     /locus_tag="PLS88_04775"
     CDS             20310..>21352
                     /locus_tag="PLS88_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOU06692.1"
                     /translation="MQDRAALTIALAGNPNSGKTSVFNAITGAHHKVGNYPGVTVEKR
                     EGKIEFRDREIRIVDLPGTYSLTAYSLDEVVARDFLLNEKPDIVIDIIDSTNLERNLY
                     LLLQLIELEIPVVAALNMADEAKEKGIVINHGILSKTLGVPMVPTVGSRGEGVEELLE
                     VAISTYEAGSKPRMPSYGEDIEAHIASLVALLKEDSAFVAAYSPRWIAIKLIEKDSDA
                     KKRISGHIREAEILAALEDTWRWIALHYRKDPEILISEQRYGYIHGAVAEAVHRTRQR
                     GIPLTEQIDNVVMHPFISLPLFFLVIWAIFRLTFAIGEYPMAWLEGLFGALARYVQNS
                     PLPNGIRDFLVNAV"
BASE COUNT         5665 a         5171 c         4653 g         5763 t
ORIGIN      
        1 cctttgatcc ttgttccatg atttatcctg caaaatcctg tctctatttc ttatttctac
       61 agttcatgtc agaatattct tggacctttg aaagaggctc atatgatata atttaatagc
      121 cggagcggtg gagcacgtgt ccaaatacca agtatgagat tcgtgaactt ggggcgtcag
      181 gacataaccc ttatgggctg ctaatcccgt atacgggagc gcctaatctc tttgtgacat
      241 atccgctccg gcgttttttt gaaaattaga gagaatcgag ctctatcatg cctcgtgctg
      301 caagtattcc atcgcaggta agtgctgaaa gccaagtagg gtgaggagcc ttaacttttt
      361 cagcctgctt caatgccttt ctatatacag ggttgcttac agcctgagct ggcttgatcc
      421 atgcgcttac tttttctcct ttcagaatct cgtgaacaaa atcgactttt attcgacaaa
      481 ttgtttcttc atctgcgcga ggcacagcat cgcatagcca gttgatgaag tgggcattgt
      541 ttacatggcc attttgatcg atgtgtcgtg ctcgagcctc tagggtaaaa gcctcggaga
      601 attcttctag cgctccaaat tgagattctt ctatgccagg agaaaggtca tcgaaaggcc
      661 aaggataatt tgttgtaagg tcgaggggaa gtgtccgctc gggccgaacc acacgtctag
      721 tctgtacatc cacgatgata tattcgtaca ttgcacccac gtatctaaaa ccagattcat
      781 ccaagagctc aagacacctc tgagcataca gtttcttcgc tcccacaggc caagttctag
      841 cgataaccct ttgtccagct ccaggcattt cctcgatacg gatgtcgata cgcgaaagca
      901 tccatgtgag ccctcttgcc cacatatcct ccataccaat gcccaaaacc gtagcatggg
      961 ctcctgccgt ctcctgaaaa aaattggcaa gtgcaaaggg ttttagaggt ccgccgtaac
     1021 cgcagtcgta tcctctcact tcataatgaa tttcaagagg ttttatcatt gatgcatcct
     1081 tatcctttta tggtctttct tatttgataa ttgcagataa gttccaaata atgttcggtc
     1141 ttctccttac attctatata ctaccttctt atatgtctct tagaaaggta ttatggggac
     1201 tcgaaagctt tttgcttatt ataaatatgt tcctacatat ggactctaac aaatagactg
     1261 cgataacgag cttggcaaat gtgctccgca gttatggaat atcatggtat aatgcatagc
     1321 tatgtgcgga attgtttcac tcgtctacaa agatgaaaac ccttcgatgg ggaaagaggc
     1381 tgaggcgctt ctcaagcgtc ttgagtatcg tggctacgat tcgaccggtg ctgcttttat
     1441 cgacgcggac aaacgaatca cactgcgaaa aaaggtcgga gctccttcca aagtctgtcc
     1501 actccttcag atcgactcct ttagtggtca gcgattcatt gggcaagtac gctgggctac
     1561 atatggaggg gtaaccgatg tcaacagtca gccccaccat gtgcattgca agatggagtt
     1621 agtcggggct cataatggaa atatttcgaa tacagataca ctcaagacct ggcttactgc
     1681 tcgcggccac gctgtgattt cggacaacga tggcgagata atagtgcacc tcatagaaga
     1741 gcactattcg gccgctcaat cgctagccag ctccgacctt gcctatctcc gcaaggctta
     1801 cgctaacgct ggcctagcag aaggcgtgcc cgacggagtt ctgcgcatga tagaggctat
     1861 tcgcagggct caagctcttg ccgaaggctc ctatgctgca gccatcgctg atccgcagtt
     1921 accaggagta ttcgctatca aatctggctc ttcactctat gctggcgttg gtagtgactc
     1981 ttttggcaat ttcatcgtgc tctcttcgga tctaacaagt gtgctttcta aaacacgcac
     2041 acttatccct ctttcagaag gcgagggaat ttggtttacc gagaacaaat accttgtatt
     2101 caaccttcaa ggtcagccat tcttctcaca tcctcgcctt aaaaggtcaa agctttcgat
     2161 caaagatact cagctttcac ccgaatacca gcactatatg gaacaggaaa tttcatcgag
     2221 cccgaacaat ataatccaga ttttacgtta ttatttccgc gatccagcca ccgagcctct
     2281 ttcggagctc ttcgaagagc acaaagacga ttgcaaggaa accgcagaca agatctcttc
     2341 gctctccgaa cgtttcggca acgagtctct acttgctgga atgaaggagg ttttcgaatc
     2401 tccagcttgg aatgaaatag aagcaagagt tcaaaataca ggtgcagcgc actttgtcgg
     2461 tcaagacttt atttcggacg agaccgaact actcaaggag ctagccgctc tcaatccgcc
     2521 cacgcgagga aaacttcttc tcatggacaa ggttatcgtc tggcgaaagc gccgtgcagt
     2581 tctgcgctat acaggcgagc tcaagaatgc catacgcgaa gctacacaat cgggtggcag
     2641 aatttacttc gtcgcttccg gcacatccta tcatgcagca ctaattgccg cttatttctt
     2701 caattccata gcccatatcc cagtctatcc ctgcaatccc ggcatgttcc gatcgatgta
     2761 tttcaattgt ctgggaaaaa acgatcttat tatcggaata tcgcaatcgg gcgaaacgaa
     2821 agatcttgtg gatatatttc aagatgtaaa aaaggccaac aattcggtgc gacttgtgag
     2881 catcgtgaac aacgaaaaca gccgaatacc tcaggagctc tcggacttct atcttcctct
     2941 tttgtgtgga ccagaaatcg ctgtggcggc tacgaaatcc tttacgagcc agattgccgt
     3001 cctctacctc ttagctgcaa gcttctctat gccggaacat aggattagag aaaacttgct
     3061 caaggccaaa gacctgatga ccgagactat agagactagg atgggagata tcgagagtgt
     3121 agctgcgcat ctatttgccg cttcttcgat tcatatccta gggacaggct taatagggct
     3181 agcaaaagaa ggcgctctca agattcgcga agtagtcctg aaccacactg aaggctatga
     3241 tgccgcagag ttcaagcatg gacccaacac tattcttggc aggaacacaa tcctttcgct
     3301 ggcagacctc gaaaaatttg ttggagccga gccattgacc gccatcaaca acgatctctt
     3361 cgagaagctt acgcacaact acccgctcat attcatatgt ccacccgaag aacgcgatag
     3421 gcgcatcaca ataagccaga tacacactca caaaattcga ggagcggata tcgtgctcat
     3481 tgccgagcct tcgcaggagt tggctctcgc agtggaaggc gcgccgatgg gcatagagaa
     3541 atattggtcg aagtatattc ccttgccaca atccggcgat ccatgccttt ttgtattctc
     3601 gactacaata gcgctgcaat acctcgctta ccgtatgtct gtacgcaaaa tgcaatggct
     3661 agattcgctg ggaatccaaa atcatggagt acacccagac gcgccaaaga atgtctccaa
     3721 gtcgatcacg gtggattaag ttgagccaga agataagcca gaatagacag taattcttat
     3781 tcttgcatat gatccggggg agaaaaatac catcgcgcac tgatacctcg gccttcgcat
     3841 tgcactcttc ctcgacgtct gagcctttcg aggcaatgcc ttaggctccc ggcatcgaca
     3901 tcctcaaaca tgagagcaag ctccctcaga gtcaaaccgc tctcttttgc attcagaact
     3961 gcatatactt tctcatcgag tcttggagat atatccgatg atatattaga tgatatagca
     4021 cctaaaaagg ccgatggctc tttcaataaa atagagtcct cgtctagccg ttctcgttca
     4081 tgcttccatg ctatagccct aaaaaaagcg taggtccaga gattcgcctc ttttcgtgtt
     4141 ccggcaaaga tgatgggaag atgtggatgc atcgcttgaa gctcggaaat gaccctataa
     4201 gcatgagcgg ctggccatct accctcgagt ctcttgggat tcataaaatc gccgtaatgg
     4261 gcctcgatta ccatagcggc gttcggtaaa cccgagagtt catccaggct tcgatgaaga
     4321 attgctatcg aatcgaaatc ggtgagtaga ttttcgtagg ttttgcgctc gactactgct
     4381 agaatcgtct cgttatgcat gagagcatag tcaccagcag gcaatcgcct ctgctcaacc
     4441 tttgctttgg gaaatttcca aggatagcgt tcggcgctgt ctatcgccac cgtcagctct
     4501 agcttggtca gccccggtct caaagcgacc cgagagcgtg atttgtgttc tttctgggct
     4561 gaatctgtct taaagaagat ctgttcgtag ctcccttcac ggtttctata ggctttttcc
     4621 aagatgagaa attcgcatcg cttcttggta ggtcgatcca ggataatctt caaacttctt
     4681 ccaaagcgct cgaaagatag aactggtatg cgttctattt ccatgaaaag atcgcgctca
     4741 gctggagacg atccatctct tatgcaaaag atattgcctt tgggtcctgg ccaggcatcc
     4801 tgggatctga ctgcgaaaaa gtcgccattt tcgtcgctta tagcaattcg atagggaaag
     4861 cgagggttac ctgttcgttc taccgtccag tacagcatac tgcccttagt gtatcagagc
     4921 tttgacattg gcgaaatgat gcccataaca ctgaatacta tggatcaaat tcccttgacc
     4981 tcgtcatcca aagccatgta atgttgacta tttatgaatg cattcaagcg ctacttcgat
     5041 gtttatcgag gtctcccttc atctctctat atgctcttct ttgctacaat aataaattca
     5101 atgggcatgt tcgtgtttcc ttttctaact ttgtatttga ctgggcggct tggaatgagc
     5161 caggagaaag ctggattttt tatcatgatg ctttacctag tctatggtcc agcttctctt
     5221 attggcggaa agctcgctga tagctttgga cgaaaaaaac tcatggtcat cgcgcaggtt
     5281 ttgtcgggac tatgttatat tccatgtggc ttccctactg ttggcagcaa tattcgttgg
     5341 ttcttgcttg caagtgtttt cttcgacgga ttgacagacc ccgctcgctc agcaatgatg
     5401 accgatctca cttcctccga aaatagacgc ccagcttttt ctctcatcta tctaggtcac
     5461 aatctaggct ttgcggtcgg aatgcttcta gcgggctttc tgtttgaaaa ctatacctct
     5521 tggcttttct ggggaaatgc aatcgcgata ttcgcagcta taacactggt tgggctaaag
     5581 gttccagaaa cgaaaccttc tcgtgggcaa atcgaaaaga gcttcggtac aggcaggcag
     5641 gatgaagctc atagaggcaa tattatccaa gccctccttt caaggccaaa tctaattatt
     5701 ttcactttgc tgacaggact ctatggcttt gtctatgcac agcatcgttt tattttacca
     5761 cttcagacca aagaattctt tggagctcgc ggttcagtta tctatggcac tttgatgacg
     5821 ctcaacgccg tgatggtaat cttactctcc acgccaatta tgacaatcac tcagcgctgg
     5881 aagccaataa atgcagtagc tttctctggc ttcttgttcg cccttggatt tggatttatc
     5941 ggttttagac cttctgtaat gcttatgtat gtgaccactg caatatggac attaggcgag
     6001 attgtcaatg ctacaaacga aggtacctat gtagcaaata atactccaat ttcgcatagg
     6061 gcaagatttc aggcattcct tcctcttttg ggtggaattg gttggaccat cagtnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcaag atttcaggca ttccttcctc
     6241 ttttgggtgg aattggttgg accatcagtc cacctatcgt tggctctttc accgaccatt
     6301 ttggactaag gatggcttgg cctatactag gagccatagc agctcttgca ggtttatcta
     6361 ttttttattt ggggttgatg gaaaaacgag ccaacaaaaa ataattgatt agatgaggct
     6421 cttattaaat ccctataatg gttcatttcc gaattttaaa atttcataac attggcgatt
     6481 ttctctcatg caatttatgc cgataaaaaa agagtgctgc atgggcgata ttggacttga
     6541 accaatgact tccaccgtgt gaaggtggca ctctcccgct gagttaatcg cccaatcagc
     6601 actcttctgc ggcatcctaa caaagcatgc cgcgatatgt caaggatggc caagggaact
     6661 agcaaattga gcaaaagccg gaaagaagca tctcctaatt ctactaaacc gacactccgc
     6721 cgtctcaata ttcgatttca atctccagct gatgaactgc gcatagaact aacgcgaaaa
     6781 tctcttcatt tactcatatt attttgcgta cctcttctag acatttcacc aatgctacct
     6841 gttttcgcat tgatagttgg aatggtgatt tattcgatta gcgaggccct ccgtcagcgc
     6901 ggcattatgg tacctgttat ttcgtcgatc acggagagag ccgcgcgact ccgggacaac
     6961 aatcactttg tatggggacc tataaccctt gctctgggtg cgcttttttc tctattgatt
     7021 tttgatcctc ttcaagccaa agtggctata cttgcgctag gccttggcga cagtttttcg
     7081 agccttgtag gtaagtcctt tggtcgcatt ccaatgccgt tttctggagg aaagtccgta
     7141 gaaggatcgg tattgtgttt cgccatagtt ttgcttgccg tctatttgct caccggtcgc
     7201 tttatgccat ctatgatcat tgcattggct acaacctttg cagaattctg gcccacaaag
     7261 gattgggata atattcttat accacttgcc gcagggatag tcgcttctct tgtactctaa
     7321 ttctattgtg ctgcattatt ctatatttct tcattgctta gactaggtgt atatactagt
     7381 cattaggcta tacccaaggt ttgatacact gacaactatt tccatatata gacatgatga
     7441 agaattttta tcgacgcaaa cacacagagc acgaatgcaa taacgacaat caatccccat
     7501 tcaaagactc ttgtgtccca aagccatacc caggaagcag ccgccgtcgt aagcgcaata
     7561 cctacaaatg cataagacct atcttctttt aatcttgctg caacaaaata atctatagca
     7621 ttcaaaacta taagcgaagc tgttacaatc attgtaagct cgctatagcc tgcctgaagc
     7681 atgaacgaag actgaaagcg atcgatacta ataggcaaaa gcatagcaaa catcgatccc
     7741 aagaggaggg cgaacaaata gcctgtttgc ggcttctctt ttccgattcc tacagcatat
     7801 aggcccgaaa taaagagcga aataagccca gaaaaccttg caccatacac taatctcgtg
     7861 agcgatatca gagaactgat ggaagggctc cgctgcgcca tgatgagcat cggaagcctt
     7921 agagcttcag cttcgaccaa aaaaatccaa agcgcaatca gatggatctc cgccgagact
     7981 gtcttttgaa atgtaaaaag cacgacaatc agcattgcag ttgcgacaag tacaagaaaa
     8041 cagcattcta aagccccagc caactgtata cccattggcg aacctagaaa aaaatactcg
     8101 gtatacaaat gcaaatcggc aagaaaaaaa gggagccttc tgtcggttcc aatgcgaaca
     8161 gctagcacaa ctgatgaaac aagcatcacc aggctcatac atgaaaaaag ccaatatgta
     8221 aaacgaaagt tgcgccttgt cacatttata tcttacttta ttccaacgat tgattgaagc
     8281 atttcttttc actatagtat gacgaattct cgcgaccgca agaggtatcg aaaatggcag
     8341 aaaaaataac tccgcgcgaa atcgattatt cacagtggta catcgatatc gttttaaatg
     8401 caaagctggc cgattattca cctgtcaaag gcagcatggt aattcgtccg cgtggatatg
     8461 caatctggga gaaaatccgc gacgaattcg accgacggtt caaagagacc ggccacgaaa
     8521 atgcctactt tccgctgctc attccaatga gttttctaaa aaaggaagca gagcatgtcg
     8581 aaggctttgc tcctgagctc gctgttgtta ctcacggcgg tggagaggag ctggaagagc
     8641 catattgtat acgcccgaca agcgagacga ttatctgggc gatgtatcga aactggatcc
     8701 agtcctggcg cgatctgcct ctgcttataa atcagtgggc taatgtcatt cgttgggaaa
     8761 agcgcacgcg gctcttttta aggacttcgg aatttctatg gcaagaaggc catacggccc
     8821 atgaaacaga aaaggaagcc cgcgaagaaa ccttgcgaat tctcgaaata tatcgagacg
     8881 tagtagagcg ctgccttgca gtaccagtag tcgccggcat gaagagcgaa agcgaaaaat
     8941 tcgccggagc ggtcgagacc tataccatcg aggcaatgat gcaggacaag aaagccctgc
     9001 aggcgggcac gagtcacttc cttggacaga attttgcccg tgctttcgat gtgcagttcc
     9061 aaaatagaca aggccagctg gactatgtgt gggcaacgag ctggggcgtt tcgacacggc
     9121 ttataggtgc ggttataatg acgcactccg acgacaaggg acttgttttg ccgcctacct
     9181 tgtctcccga ggatgcggtt attattccca tctatcgcaa cgattcgaaa gctgctgtta
     9241 ttgcgtatgc caaaaagctc gaagccgatc tcaaaaccgc cggctttaaa gttgtgttgg
     9301 atatcgacga ttcatctagc cctggctgga aattcaccga atgggaactt cgcggtacgc
     9361 cagtacgaat cgagatagga cctcgcgata taaaatcgaa tcagattgta atggtgagaa
     9421 gagatacagg cgaaaagctg actgcactca gcaccgaagc cgcaagcaag ctcaaagaat
     9481 tgcataatct aatacagagc tctctgtttg agcgggctcg atctttccgc gaaaagaata
     9541 cagagccgat ttcagatctt ggtggtctca tcgaattttt cggcacagag actgcgggca
     9601 ctgaaggagc acctggagga tttgcagaag ccctctggtg tggttctgct gcctgcgagg
     9661 cctctatcaa ggaaaaaacc aaggctacaa ttcgctgctt gccgctgggc aaacaagacc
     9721 atatagaagg gacttgtgcc atctgcggag atcccgcaaa gtaccaagct atttttgcgc
     9781 gtaactattg agcgccgata tttgcatact cagtatttag ataatgaata aaaagcagga
     9841 attctcatag tccaccggcc gatgtactct cccaaagacc ttgaatatag agattcactg
     9901 tagcattttt agaagaagta aggacaatct gatcgtcctc tttctcagag ccacttatgg
     9961 caatagcgat aggagctggc gtcgtagacc atgagtcaat cagcgctaca ttaaaagagg
    10021 aacggctttt ccaaaagctt cctacgaaga agctgacaac aagggcagtt atcgcaagag
    10081 ccatcgaaat caagaattta ttccttttgc gcgactgctc ttccgccttt ttgtaaacct
    10141 gcgcaaaatc gggaaaggca ggtgctttcc tcatagctgc atctttgagg tcagccttta
    10201 gatcgatttt attcttttca gtggagctca tatcccatct ccttgagcat ctcactcaat
    10261 tttgcccgtg cacggaaaag tcttgatttg actgtcccaa gaggaagatt gtacatgctc
    10321 ccaagctcag atagttcaat tccctctacc tcatgcagat agagcatgga gcgatcctcg
    10381 ggctccagct ttttcatgac aagctcgagc atctgactag cctcttttct cagcatatcc
    10441 tcctcgacca acccatgcgg attctcgata aatagagatt cgaattcttc cacagaaaaa
    10501 tttcgcgcat ggttgcgagc attgacacgc aagacatccg cgcagcttgt tcggcaaata
    10561 ccatagatat agccgcctaa agaactctct ttacgccact taggcaaggc tttatagata
    10621 cgaataagcg actcttgcac tgcttcctcg gaaagctctt catcgaatgc aacaaagctg
    10681 gcacaatagc cccgaatacg atcccaccaa cgccatatca ggcgctcgaa tgctccttta
    10741 tctccctttg ctgctctcgt tgcaagctcg atatccagag tttcctcgtc ggacgaaaaa
    10801 gcaagagatg aagcttcagc cttgttttgc ccaatttggg ctctatcttt ttttgctggg
    10861 ctcatagtcc atgccattct attttcgctt ttcataaata taatgcttta ttatatcttc
    10921 cattacatta ttctgcggag tatagcaaga agatggctgt atcggtttga ttcttcgaca
    10981 ctaagccctt tggtgctgaa ggaattcgca aatttccctg ttttttccga catccgagct
    11041 tcatgcacaa gaagcagaat ggtctgccag cgatcttcgc caactacttt tcggatagct
    11101 acttgcctct cgatttgtat cattcttaca gtcttttcat attggaggct cttcgagatc
    11161 gcttcttcta tcgcttttag gtcaggatct ggatcgacta gaatgtttgc aatctgtgct
    11221 tgtagtattc tgatctcgtt ctgcgctttg ataagctcga attctctgga ttctagaatc
    11281 ttttcgagct tgtcgagatc ttcgtttgtt agattcaaac tgcgaaacca ctcgcgcacc
    11341 actatggggc ccataacacc ctggtttccc attccttttc ctgaacctgc ctgtgcagcc
    11401 acaggaagaa taccgagaaa cgcaatgctc gtaattagga tgagcaagac tgatagcctt
    11461 gccctatttg ctttcatttg aatcctccgc tacctttaat tacatatacc tctaattaca
    11521 tatccttctg tataataagc cacgcagctt cggtagccgc gtggccttta tcttagatat
    11581 ccaattggct gcaagattct tttagtataa atcgagcttg gataacccgt aagtcagcta
    11641 ttaagctagc tggtttctta actatttgca gattgaaata ttttattaat ctctagaatg
    11701 ctgtctgcgc gaatctcctg tatttgtttt tgaatatccg caatcttggc tattgtttca
    11761 tctattttag ccttgtctgg atttgggctc caaaggagat tgtccaaatt cagcttttct
    11821 gcgcgaagct cattccggag cggaagaatt ttggaggcat tatcctcaat gatcttatct
    11881 accttggctt tgttttcgtc tgatagcttc tgatactgga aatagtgaaa tgccccatag
    11941 ctacccgcgc ccatagaccg catgccgcca aagaagctct gctttggatt agctaatcct
    12001 tttgatgcat cgccaaatct attcccataa ggatcgacca ttggggaggc cacaggcggc
    12061 atgctttgat acccataccc cggagtcctt gcgcgaggac ttggcattct gccaaagcct
    12121 tgagcaaaag cacccgcagt tgcggccact agcatgaccc ctaccataag aaacttaatt
    12181 gcttttctcg atgttctcat ctttttcctc cgatgaagat ctttgacttt cactataatg
    12241 tcgcaggaaa gggaatatcg gttgcatgga aaataaaact ttgccatgat gggatgaata
    12301 actccagaca gaatttaaaa ggaagtttag gcaggatacg ctaaataatc tagacagggt
    12361 ttacgcgata attcggacag atgcacagaa taatttcgac aggtttatgg gattatgcag
    12421 gattgacagg attttttctt gggcaaaaag aatagaatcc tttcctcttt ttcttcgcca
    12481 aatactatct catcccttta atccgtcttc atcctgctca tccggtcaac acactatccc
    12541 ttccatcctg tcattctacc caatcttcat aacactaggg acacttttct atttaagcgc
    12601 attgagttaa tctataagaa aagagagccg gaacataagg ctttttgctt ctctatgctc
    12661 tcttccataa tcattttcca gaggagtttc gcatgccatt tgtgcttcaa aaagagtcct
    12721 cggtgcctga actcgattcc ctgttccgac tttatcgcca cgaaccaacg ggagctcgac
    12781 ttctatcggt catcaacaag gatgaaaata aggttttcgg gataagcttc agaactccac
    12841 ccgaacgaaa caatggagtt gttcacatta tcgagcattc caccctttgc ggttctcgca
    12901 agtatccagt aaaggaacct tttgtcgagc tgatgaaagg ctcactaaat acttttctca
    12961 atgcaataac attcgaggat aagacctgct acccggttgc aagcacgaat ctcaaagatt
    13021 tttataatct catcgatgtc tacttagatg cggtcttcta cccaaatcta accgaatata
    13081 ctttcatgca agagggctgg cattatgaag tagacccttt gaccaaagaa ctcagctata
    13141 aaggtgtcgt attcaacgag atgaaaggcg cctattcaga cccagatagc ctccacgatc
    13201 atctatgcaa atgcagcctg tttcccgata ctccctatgg cctcgattcc ggcggcgatc
    13261 ctctggagat tcctactctt agctatgaag aattcattgc atattacaag aaacattatc
    13321 atccctccaa ttcttttatc tatttctatg gcgatgatga cccagaaaag cgcctttctt
    13381 ttatggataa atggctatcg ccgtttgaaa ctgcttccat cgactcactt cctgctatcc
    13441 aacaaacttt ctcagagcca cggctcatcg aacgcagcta tcaaataaat tcaaaagaag
    13501 aagccaaagc ctatacagcg cttaactgga tgctatatga acatggagat cttctccttt
    13561 cgatgcaagc tctcgttctc ttccatattt tgactggtac tccagcctca cctttacgaa
    13621 aggctttgat agagtccggg ctcggagagg atattgcagg ttttggcttt tacgaagaga
    13681 tgcgccagac ggcgttctcc attgggctta agggagtaaa gccgaacaat gtaaccaagg
    13741 tagaagagct tattttctcc accctcgagc ggctcgcccg agatggcata gatcccgaca
    13801 ctatcgcagc ttctctcaac accatcgaat ttgcattgcg tgaacgaaac actggctctt
    13861 ttccgcgtgg cctcgccatc atgctggatg ctctaaatga atggctctac gacctcgatc
    13921 caatcgcggc gcttgctttt gccgaaccac tcgaacaaac caaaaaagct gttaaagaga
    13981 acccgaggct attcagtgag cttatagatt cgcttatctt gaagaatagg cacagaacca
    14041 cagtcaggtt tctgccttcc tccagttcaa accacgaacg ggaagacgcc gaaaaagcta
    14101 tgttagctct agcccgagcc agcttagatg acgaagcgct taaaaaaatc gaagatactg
    14161 ccaaaactct caaagaactt caagacaagc cggatagtcc tgaagcgctc gccacaattc
    14221 ctgtactctc gctcgacgat attccaacag aatcgcccat cctgccttct aagcccctcg
    14281 aatcggcaac cgataagctc gaaggctctg gaatctatgg ctactatcat gagctttcga
    14341 cgagcggcat cctttacttg gatcttggtt tcgattttga aaagctgcca tctgaacttt
    14401 tgccttatgt tagtcttttg ggtcgtcttt taatcgagac aggcacggaa aaatacaatt
    14461 ttgtccagct tatccagcgc attggcatgc atacaggcgg aattcgaagc atggctatat
    14521 ccacaacgca ttggaccgag cacaagccag ttccatggtt tttcctacgc gccaaagccc
    14581 tgcccgaaaa agtcggcatg cttactgata tactcttgga tattctcatg agtgcacggc
    14641 tcgatgaccg tgaacgcatt cgccagattg tgctagagga gaaagcacaa gccgaggcaa
    14701 tgctcattcc cgcttctgcg cggattgtcg gtcttagact tagatctcgc ttcaatccgg
    14761 catattgggc ttcggaaaaa cttatgggta tagaacgcct ctttttcctt cgcaagctct
    14821 tgcatcgtat cgaagaagat tggccgacag tattgtcaga aatacgagag gtgcatagag
    14881 aacttattcg gcgtgataat ttactcgtca atatcacttc ggatcgaacc attctcgagg
    14941 catccgcgga gtctattcta cgccttgcgc agtctctgcc tatacaatcg acgcttgcgc
    15001 aatctttctc tgataagcct tccttcgagc tcgattcatg gataaacgaa gcatcgcatg
    15061 cttgcgaccc ttctatctct agaacctcta aacacctaga gaatatttct ttagaagacg
    15121 aatctgtcca cagcgctcgc cccgctctcg aaacgatcga gctgcagacc caggtgaata
    15181 gcgtaggcat gattttaccg ctaacagggc tcgatcctat tgctggcgga gccctcgtag
    15241 cgacaaaata tcttgatacc tcgtatctat gggagcaaat aagagtcatc ggtggagcat
    15301 atggagggtt ttccagcctc gatctggcct cggcgttgct tatgtttctt tcctatcgcg
    15361 acccaaacct agatcgcacg atagaagtct accgaaaggt gggggctttc ctggaatcct
    15421 gcgatttgcc aagagaagag attcaaaaat cgattatcgg tacgattgga gatgtcgatg
    15481 cctaccagct gccagacgct aaaggattca acgcccttat gaatattctt tccgggtaca
    15541 ctcaagaaac gcgtcagcat atccgcgacc agattcttgc agcggacata gaggatttcc
    15601 acagcttggg aaagctactt tcggaagcgg ctgagcattc gatgatagct gtaatgacct
    15661 caccggagca agcagagaag tcgctcttaa cgctaccggc tccggttgtg cggctttctg
    15721 tacactgata gtaaacagcc gcccagcttt ctgtacataa ttagcgcata gattcgaaag
    15781 ctttccagtc gagcttatcg agcaaagctt gatctccaac agcgacccaa aggaaagtgc
    15841 cgttatgcac atatttctcg aaggcattat agactgccga ggcatccagt atttctatgc
    15901 gattctggtc tttaagccaa tcggccggat ttcctgtcag ttttatcgag cgaatcatga
    15961 gagaggctat ggcggcattg gttcgaacag cttcgaaata atcatttatg taaagctgct
    16021 tgtaggtatc gagcgcatct gagagcccca tatagccatc ggcatctgtt ctggtaggat
    16081 ccactgacac aactctacca tttgccatga gagcaaccgc ttcatcgatg tattgcttga
    16141 ttttttgcgg tgcgtctgat ttataaatca ttatggaacc ataatttgct gtgtgtgacc
    16201 taatagctgc cgaaggagtg tagactgctc catacttatc gcgcaccact gcgaaaagca
    16261 aatccgaaaa aatgcgtgtt gctagggttg tcgcaaaata atcatcgctt ccaggcgctg
    16321 gcgctgcgaa atcgcccctt agataaatta tccccttcga ctgatcgtga gcttgcgtga
    16381 tgagagatcc caaggctaat tccgatccag aagatcccat tggaaaattt ggaggttttg
    16441 gtactggtcc aagattcaag ttaggaatat caccaagaag cttttcaagc ttgtacgcaa
    16501 gctcggctgg attgaagtct cctacagcca caaggaaaat tctatctgcg gaaaaatgct
    16561 tctcgtacca acctcgagca tctccaacca tcattggcgc aatactctct tcggtacctt
    16621 cggggttgac tccataggga tgccccttga aataagcctc gttcattatc ttctgtgtca
    16681 tcgtccatgg attctgctct atcgcctgca gcgcaagcaa ggcattctct ttttctttgt
    16741 caaaatcgga ctgatcgaaa gaaggattta cgagcatatc gctccagagc gcaagaagtt
    16801 catcgctata cttgtcaagt gttgtaaggc tgaaagttga atattcgaat tgtacggcac
    16861 ttgtaatggc cgacgatctc ctatcgagga tcgaagcggc cttctcgtaa gggtaattct
    16921 tcgaactcct cgccatggtg gctagggcaa ttttctccca gcccgcctgc tctggagcag
    16981 tcacaagaga agctccattg aggacaagcg agacgtgaaa tacctgattc gcatgctgca
    17041 ctttcatata cagaggaatg ccattagaaa gcactgtatt catgtatgta ggagcgtttt
    17101 ctcgaacata gggagtagtc gcacaagctc cggtcgctac tcctactata atcatgacaa
    17161 caataaatac cctaagaata ttatgactag aatgcctcat acctgcctcc tcattgtttt
    17221 tgccaccaga aggcattttg cgcagtcaaa agcgcatacc cgagttcgga aatcttcgcg
    17281 ctcatgcgtg gatctgcatt gaatgtgctt gtgcgcagcc gaacaagggt agcgaccgaa
    17341 ggagtcgacg cccctgccag atatctctct ataaggctag agatgtctct ccagctaacc
    17401 ttgcggcaat tttcctcgta atcgaagaaa tattcggtgc ctgcggtaga ccaccagaat
    17461 gtgagcgtat cggtaacaaa agaccctgcc gattcaagag cgtagatgtt ctgatctacc
    17521 agctttgtct tggcttcctc tagggcctca tctcctccaa aatacgcgca gggatccttt
    17581 gctatgagtg cgaattcatc gagcactgca gacctaagat tctccactcg ctgaagtacc
    17641 ggctcttctt ccgttggcct ttgaacgagc attagagtag aaaaaatgta attcccccca
    17701 tcgcgcgagg ttggatagac aaaatctatg tattcggcat caaagaggcc atagactttc
    17761 cgcattatct ccgatttgaa aggccctgat ggagaactca ataagaacaa aagcacatcc
    17821 gatatatagg tgtcgtaggt ctgacgtgtc acgtcgggtc ccctccacct gaattcaact
    17881 tgagcgatac cccgatagaa ttgatcctct gaagttacaa gacgaatacc gtcggggata
    17941 ggtccatgag gtggctcggc tagcacaggc tctggcgctc ttgcccaact tccaaaggat
    18001 gccttggcta gctttaccac ttcttcaaga ctggtatccc ctcctatgag gagcgccata
    18061 ttggaaggca catagtaggt cttctgaata tctcgaagcg ccgttacgct tgctatttgt
    18121 atattcgact caggaccgtc aatattcttt cgccatggaa aatccttgaa catacgcgat
    18181 tcaagaccat tagaggcaat ctgtgacggg tcgctatggt atccacggat ttcattcagc
    18241 acgacctgct tttcgttctc cagaactctg gcatctagag ttggattcat gacggcatgt
    18301 gcccaaaaat cgattgcatc cgggagcttt tccgaaggca cgctgatgaa atagtttata
    18361 tattctatgc ccgtagctcc attccagttt gttgtgccca tgcggtttag cgcagcagag
    18421 aaggcatctt ttgtcgggta tttgtcgttg ctattgaaca tcatatgttc atacaaatgg
    18481 aaaagcccag cggtctctgg tgtatgagcc atggcgcctc cacgaaaggc aatacacacg
    18541 gtaacaagag gcactgcatg gttttccaca acaaagacct cgagcccgtt ctctagcgtt
    18601 gtattccgaa ctgcctctgc ttgttgggca acaacgatat ccagcgttga cagcataaaa
    18661 aaaagagaca gcattatgca aaaaaaagaa tttttatgtc tcatttgctt ttatgacctc
    18721 ctttctaaac taatgttcca agctaatggc ccaaagtggc gtttttgtcg tgctcatcga
    18781 tgtcccgagc atcccaagga tacacaatcc aatcgtctcc aagattttct ccaacaaaga
    18841 catgctgaac gccaggagga aatgtgccgc gtttctcttt ttgcttgtta tgcagaaccg
    18901 agaccgcgag tgctgccggt ctataggtga gtagttcctt agtacaatat tccagcgtga
    18961 cacgagaatc gtccacttcg tccacaagga gaattcgttt gcctgccaac tgcttttcgg
    19021 cctcttcgat ccactgtacc ttgcgaggat agtccattgg cctatcattt aaatcataat
    19081 aagctattcc aacggccagt atcggcttgc caagataagt ccttagaatt cgcgctggaa
    19141 taaaaccacc ggtaccgatc gccacaatta catctggaga aaagccgctt tccttgacct
    19201 gctccgcaat ggactgcgcc agcttatgaa tcatctggta gctatagtaa agctttccca
    19261 tattacgatt ctacggcaat ggtatagcaa ggacaagaga tatcattaaa aatcatcttg
    19321 acttttttaa gtgaaaatga ttatcattct caaagagcga tagaattatc attttcttcg
    19381 cttcttattg cttttacttt cttctttata atttttggta ttggataagg caatcaaaat
    19441 gaatgaactc gacagattcg agacctttct gcgcgagcat gggcagaagg tcacaaaacc
    19501 gcgtagaatc atactcgaag cattcctcaa taccgaaggc cacctcagca ccgaagacat
    19561 cttgcgagaa gcaaaaaaac acgaccctgg aataggtcag gctacggttt tcagaactat
    19621 tcgcctaata gaagatgcgg gactggctcg cgaagccgtg caggaagatg gtgcacgcac
    19681 tttcgagcat cttgccgatc accctcatca cgaccatctg ctttgcactg aatgtggcaa
    19741 gataatcgaa ttcttgagtc ccactataga gaaagaacaa caaaaaatct ttgtaaaata
    19801 tggatttaga cctataggtc ataatatgga actgctcggc ctctgccctg agtgccaggc
    19861 aaaagaaaaa ggagcccaat gatatgcagc ttgccgaact tagtccgggt cagagattca
    19921 aagttataaa cgttgcagtc ggaggagaaa ttggaaaacg tctcgcggat atgggtcttg
    19981 tctctggagc tgaaggagag gtagtacgtg cagcattctt ccgcggtcct atacagatca
    20041 ggcttggaga ctacgatctc attatgcgtc gatacgaagc tcgccttgta gatgtcgaaa
    20101 ttcttccaga agccgcgcca cagatggcaa gcaaggcgcc taagaaagca ctggccacaa
    20161 tgtatgtcga tttgcgccct cgcggacgaa ggcgttctca tggcgcttat ggtgaggcta
    20221 agggcactca tggcacgcat ggtaagactc atggcgccca tgacaaagat cctagctgcc
    20281 tcgagtaaaa gacttttcgg gagtaatcca tgcaggatag agccgctctc accattgcgc
    20341 ttgcaggcaa tccaaattca ggaaagacca gtgtattcaa tgctataaca ggcgcacacc
    20401 acaaggtagg aaactatcct ggtgtcactg tagaaaaacg cgaaggcaaa atcgagtttc
    20461 gggatcgcga aattcgaatc gtagatttac caggtaccta tagcctaact gcatattccc
    20521 tcgacgaagt ggtagcccga gacttcttgc tcaacgaaaa gcccgatatc gtcatagata
    20581 tcatcgattc taccaatctc gagcgtaatc tatatcttct gcttcaactt atagaattgg
    20641 aaattcccgt tgttgctgcg cttaacatgg cagacgaggc caaagaaaaa ggtatagtca
    20701 tcaatcatgg aattctctcc aagacacttg gagttccaat ggtacctacc gtcggttccc
    20761 gcggagaagg agtcgaagaa cttctagagg tagctatatc tacttacgaa gcagggtcaa
    20821 agccacgtat gcctagttat ggcgaagaca tagaagccca tattgccagt ctagtcgctc
    20881 ttctaaagga agatagcgct ttcgtcgcag cctattcacc tcgatggatt gcaataaagc
    20941 tcatcgaaaa agattcggat gccaagaaac gcatttctgg ccatatcaga gaggctgaga
    21001 tccttgcggc tctcgaggac acctggcgct ggatagcgct tcattatcgc aaggatccgg
    21061 aaatcctcat atccgaacag cgctatggtt atatccatgg agcggttgca gaagctgtac
    21121 atcgaactcg acagcgtggc atcccgctca ccgaacagat cgataatgtg gtaatgcatc
    21181 cttttatcag cttgccgctt ttctttttag ttatctgggc aatcttcagg ctgacatttg
    21241 ccattggaga gtatcccatg gcctggctcg aagggctttt tggagccctc gcaaggtacg
    21301 tccaaaattc gcccctgccg aacggaatca gagattttct tgtcaatgcc gt
//
LOCUS       DAOSOB010000031        21132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_2505_length_21132_cov_70.365944, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000031 DAOSOB010000000
VERSION     DAOSOB010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 21132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21132
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2505_length_21132_cov_70.365944"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1583
                     /gene="secD"
                     /locus_tag="PLS88_04780"
     CDS             3..1583
                     /gene="secD"
                     /locus_tag="PLS88_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOU06693.1"
                     /translation="MTYLIISDLKERAAAGDTTDLSNQKNYKVAIDSAKKAYSLAKKP
                     APKTWDAVAILSAFSSERSMFDAIESRYRERVLKLKNVHSNAVQLGLDLAGGMSAVVQ
                     ADLKGLSEKLGHELSQAEKEDAMKRAVEILNSRIDKFGLTEPVIRRQGEDQIYIEIPG
                     TPDPERINSIIMGKGNLAFYIADLEASAAVNSYLSTNPVGIDEETMTLDKPGLVPEGK
                     IVRKVYKKDSYGLDEFTGEYLVLEGKPGLDGSHIVSATVSSDPITGQPETNFVLDKEG
                     GDIFYELTSANVGKTMAVVLDDRVKSYARIQEPIRESVRITGFSAEEAEALALLLRTA
                     ALPISLSVVSQQSVGASLGEDAINQGQTAIIVGILSIFVFMFLYYKWAGINATVAQIL
                     NLYLMLSVLTAFKLTLTLPSIAGFVLTTGMAVDANVIIFERIKEELRAGKSRAAAIHA
                     GFDKAFWAVMDSNITTIIAALFLAQLGTGPIQGFAVSLAIGNITSLFTSLFVSRLIFD
                     FETDVLKLKTVSISWRIR"
     gene            1583..2833
                     /gene="secF"
                     /locus_tag="PLS88_04785"
     CDS             1583..2833
                     /gene="secF"
                     /locus_tag="PLS88_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOU06694.1"
                     /translation="MKNIIKFSKFFLPAVIISLSIIIFGLVGLFTKGLNLGVDFQAGI
                     NETIQLAYPAGDVTFDGKGNAELTVSESQLTLVFSGAEAQKRTVVFDYKTYSTLADIK
                     SALEKEQGIRVSLAEGSESLPSNLLVPTYQGNSLLSLNPVKLYRAPKDDSERYASIDI
                     VREAISPLGDITVQTINPPSSQRYLIRVRDSGEDPQFMTNTPQTIRKLIEDKVGTDRV
                     VVVKADYVGPQYSEALGKQSVWLVLMTLLLILIYSAIRFKIEYAIGAVVAIAHDALVM
                     IAFIVWTRMEFNTTTIAAILTILGYSINDTIVQFDRVREERKLHPSEPFVDVLDRALT
                     LTLGRSIITTLTTMLAVLALYFFTSGSIKDFALALFVGMVSGVYSTIYIASAIVLFWE
                     NKKTKRQAKAPKDKGAPAAAVSKN"
     gene            2932..3003
                     /locus_tag="PLS88_04790"
     tRNA            2932..3003
                     /locus_tag="PLS88_04790"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2964..2966,aa:Cys,seq:gca)
     gene            3196..3660
                     /locus_tag="PLS88_04795"
     CDS             3196..3660
                     /locus_tag="PLS88_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06695.1"
                     /translation="MKKKVFLSVLAALLISVPVFAATQVETTIQATIGADLSITTNIP
                     GTKAIDITASSTTLGSVTISSNLTNWKIVIHSAKGGKMVRVGGSDVYPYLLNFGSTTG
                     IDLASDYEIVKTAPQSPTSTTVVVTYTKASELNPALPAGTYEDTLTISLVAL"
     gene            3742..5313
                     /locus_tag="PLS88_04800"
     CDS             3742..5313
                     /locus_tag="PLS88_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU06696.1"
                     /translation="MSPISSQKKNKAKMAKVLLPLFFCILAIFCFFMLWKSANRSESE
                     LLGAVSKRIALQISEPLQEMLAVGDKFRELLLTGSGKTYAALRPLAEETLANLPYIDS
                     ITIAPGAIVRYFFPEGRASSSIGHDLLDNPERMDALVEAVRRKSAVLQGPELSAEGKN
                     LAFLRIPVIEGSELWGFVSIAFNADKLLSDLDLVSQFPGLSIAIASSNMEGGDRRLFW
                     GEERALTGYSAVLKIGSEDFPWTIYVASMYPYKRTGLWGVGLLILVLLSCGLVIRAEF
                     LEKESSKAPVRPKETSIAIKPFVPKTESMQEPSIELVKKVDIPKASAMEESMAQEIVA
                     EKLASKESIAQEPASEKLVSPSLEKPIKEPANGSPKDMAVFQNQAIENEAEKKEVAVS
                     MEEIPGSISVLVVDDSEVNRDLLLRMLTLKDYKAKAVSSGEVALKALCEESFDVLLID
                     CIMPEMDGYALAGKIRTEGISSMPVLIAMSPRHDQEEAEKCANAGFDNLLVKPFTMTA
                     LDQQIRLALGNRNAE"
     gene            5680..6159
                     /locus_tag="PLS88_04805"
     CDS             5680..6159
                     /locus_tag="PLS88_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06697.1"
                     /translation="MKKYALVALFAVVSLGMVFAVVANPGSGTVSIQGSVDQYFSLTV
                     PASYTGSISYDSSTHWDLSDMKVISNVKNWVLKASSQNNGVLKDTANGADIAYKVTVG
                     SLFTKQQLTSGGITSGPQSKTPKAGTAYKFSIDIDAPSELYQAGTYSDVITLTISNQ"
     gene            6339..6971
                     /locus_tag="PLS88_04810"
     CDS             6339..6971
                     /locus_tag="PLS88_04810"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU06698.1"
                     /translation="MNRNSVLRRPFKFKYFNATLYLIAVNVLIFALQYIWPLLTYLLA
                     LNPGAVASGWVWQLFTYMFAHANLTHLIVNMLGLYFFGRPVEHSLGSYEFLLYYLGSG
                     LFAGIASFAIYLLSGAWNTYLLGASGAIFSLLLAFAVLYPRAMVYVYGIIPLRAPVMV
                     IGYTLIEIFSSFLGAASSVAHLTHLAGFVAGAAYFPIRLGVNPLKRLSDR"
     gene            complement(6958..7851)
                     /locus_tag="PLS88_04815"
     CDS             complement(6958..7851)
                     /locus_tag="PLS88_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU06699.1"
                     /translation="MDTIKSKYAPLVGEIIARVPKEARISELNVYQEGIGQILPFIDA
                     IIADHLEDGSKIEGLDNLKSLYEHAKNGESTLLLMEHYSNFDLPVFHYLLRQEGEEGK
                     KLASDIVAIAGIKLNEENPAVNAFTRAYTRIVIYPSRSMQIIREKLTDPIELYQEMKR
                     SISINRAAMRALEGAKHNSKMILVFPAGTRYRPWDPSSKRGVREIASYIKSFSKFCLV
                     SINGNILRLNPSGAMEDDILQRDRIIYNVSPVHDNKEFLNSVRYDLRFLEDKKQAIVD
                     TVMDLLEDMHYEVENRRFSDR"
     gene            7954..8790
                     /locus_tag="PLS88_04820"
     CDS             7954..8790
                     /locus_tag="PLS88_04820"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOU06700.1"
                     /translation="MAIVVSASRRTDIASFYTDWFFERLGEGFVDVVNPWNPRQIRHL
                     SLRPEDVACFVFWTRDASPMLRNLSMLEKYAFYFQVTVNGYGVPMEPSGPGVEAGISA
                     LKALSSELGSRRVVWRYDPVLLTEQYNSAWHVENFKRLAGEIGERVRHCVLSFYDSYM
                     HSDKRLSKAGYRVAPVDRASNEVRDLMFALVGIARENGLSLSLCAEPELKEMLGLEQY
                     ACIDACIIGELSRLPFSSKRDKNQRKGCQCIESVDLGSYGTCPRGCLYCYANRCKANI
                     SQ"
     gene            complement(8850..9668)
                     /locus_tag="PLS88_04825"
     CDS             complement(8850..9668)
                     /locus_tag="PLS88_04825"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU06701.1"
                     /translation="MDNFHSSSLMPITSLTSQEARKIRYVLMDIDDTLTTDGKLLAES
                     YAALWRLHEAGLKVIPVTGRPAGWCDLIAREWPADGVVGENGAFAFWEGEGHHLLTLS
                     HPNAILNTDPRLLALRDRVISEIPGARLARDQFSRLYDIAIDFAEEEPILPLSEALRI
                     QSIFLEGGARAKISSIHVNAWMGDYDKLSMALSFLNARFGYDDSRDVGTVIFAGDSPN
                     DEPMFEHFPMACAMANVLKYRELMKTPPHFVTQRESGAGFAELADIFLKRRSVS"
     gene            9654..12899
                     /locus_tag="PLS88_04830"
     CDS             9654..12899
                     /locus_tag="PLS88_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06702.1"
                     /translation="MKIIHIKLWYNNDKNIINKGLSCVKKSMVNLSMVIIRFFYQCLC
                     NDCFRKIPGHLKNLCNWLNHKLDNKRYANRPAIIALLFFCTFVRVHAQLIPSMEPVLH
                     LKTDSFDIYSPKSLEAEAKRLASFADKTYGELCDFLGLELRTERIPVLLSDLQYSLNG
                     FSTLYPSNRIVLLLASADPRSQLATLEDELYSVFLHELVHYITLNERSLAWRVLSWFG
                     GDWVAPEIYMMPQAMVEGTAVWVESRFKENGSGRLNDPAALEFVRLERARGKDRSLWD
                     VSGLEDFYGAGSLPYLYGGLFVDYLSERFGPSIVGRLWQSSGRGIIYRGFDGGILSKG
                     ILERETGVKPNKLWQDFLVWIDEGSELEVQEGQGGVDPIEMNDAADMPAEKELFSGYV
                     GALGAGGGKVYFVDLERRGMYQLSVVDLQEDSKNKSEFEKKAMAKKPERLFATDQMLR
                     NIYCNVKFGRLELDWTRINSQNQAIPARYIYDLSEHKLRYEKDLPQASIGEVLFEKHS
                     GTKEEISLYDSWQDPLTSIEYGLVRLGTAVLPARRFANGQIEVADIPDLAIRWLSQGF
                     RENSEPSDSISFALSLVPDNGLSRLAILEQNNGLWRFAIARKSPSGGVHMPVFTDASH
                     LVYRASIGNGQSRLLLLDISDFKDEIEAPGQHFDSEPLRWISPTAWIESYSLEMRNQS
                     AHLESSEEVQHQLEPNREVQLQPETSKVPQPEPELSSELSLEHSKAVQKRSTLFPAIF
                     ETSRIPYADGTLLGIDFIATDLTERLSWSALVGWDFLIAKPATSIAFQLSAGAWRFEL
                     SAWDQGVFAFPVARISALEGALHWGHALIPTFKSISTEIHARYGGVQNKYSEQGLFYL
                     VPDYCAWVAGAGVEFDSLSSSYKPPYNPSGISLKSGIEYEGANSSNFGGLSISGSIYL
                     GESTIAERIGASIYGAWTPTGGVAFSPAARYLENAGNYEFSVADIPYPAYKEYEACSD
                     LSSWYLYGEIKARLFSLELNWIMSHLFAPSFALRRISMSSGLRGAGLEIDNTPSLLSS
                     AFLQADFDCALLSGLAAVTHMHLTLEAAWAFQADKADVKPFHFSVGLAAGL"
     gene            complement(12969..14141)
                     /locus_tag="PLS88_04835"
     CDS             complement(12969..14141)
                     /locus_tag="PLS88_04835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOU06703.1"
                     /translation="MKNFTWYTPTKVIFGTKILDQLGREASRIGKRALFLYGKQSIKR
                     SGLYEKVVAQLRGADIEFMEHGGVQPNPIISHALEGAQKVKEHELEMIIAVGGGSVID
                     EAKSIAVASCNEEPLWDFYLGKAIIKNALPIIAVQTLPATSSELNPTSVMTNDATGEK
                     FSFSSELVFPKLSFLDPSLTLDIPIQYTAYACTDILSHMMEGYFTASEDSPLQDGIVE
                     GTCRAVIGALDTVMIQPRDLKARSTIMWAGALAWNGLFVAGVEGASTPNHMLEHPLSG
                     LYDLPHGAGLSIVIPAWLKYMKPHIAHRIVLFGERILGMGESLRNKAALDAADLVIEE
                     LEGWYRHIHTPIRLHEAGIEKLDIEACTKQALSLSKLWNIPGYKDSDIQAIYEMMA"
     gene            14438..15400
                     /locus_tag="PLS88_04840"
     CDS             14438..15400
                     /locus_tag="PLS88_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOU06704.1"
                     /translation="MEAMFSPPSDLGAYDLRIGTVGYEYPEWEGVLYSHGTDKEAYLR
                     LYCERFSTLELDFTFHTMPKAAQMRDILARALGSSAGQAQFQNQLIQKLPVQEPSGRA
                     PTDFSVIAHSSLTHRIDPFTWSSSLRTFLKGIEPLAEADRLCAVLLVFPFSFRYREEE
                     RRYLDKLLKALETYPLAAYSLAVEFQNSEWLTLRVIEELKNRNVALCALDMPRMEGRP
                     PISDIVTSDLAYIRFLGRAAPGVLTDSSNSSSIEEGARNTEEGLWCPDLYRYKSEELA
                     LWIPRILSMSAQASKMRIFFGNRRKGNAPLDACAFTKLLRAAGL"
     gene            15441..16625
                     /locus_tag="PLS88_04845"
     CDS             15441..16625
                     /locus_tag="PLS88_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06705.1"
                     /translation="MSTGTILHINAVGFPAAIEMNLDRSLKAWPFVVANERAPRAVVI
                     APSPSAHREGVRPGMVLSMAKSICPKLAVRPPQPELYRKIEDTLQKLCLSFTPLVEHA
                     GTGHLYLDLAGTSRLFGAPEDASQKLRARIYEQTGLTPTLGLSSNKTVSKVATRVFRP
                     SGFVALSANEESQLLRHQPAAILPGIGPVLLGRLSLLDIEEIGQIADLTAGEARTLGP
                     RGLELVMRARGIDSSPVDPEPVERKTVDDTIVFEPDTSDPEIMKIRLRSLVAELGFAL
                     RKANVGTHKASVAISYTDGPTARATARTSQLLVRDDEIFSLAVLALERAKKRRVRVRR
                     LELILSEIDAAGANLDLFEPEEIRLFRFQSALDKVHSRFGFEALVPCSALLVANSLGG
                     QS"
     gene            16622..>18419
                     /locus_tag="PLS88_04850"
     CDS             16622..>18419
                     /locus_tag="PLS88_04850"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU06706.1"
                     /translation="MIAPLFVNSMYSLLYGTASPEALAKKAAALGYGALALTDRNSVY
                     GLPAFIEACKQERLRPILGTELVYRETRQENTKEAAQEARKEYKQEVKKEIMQEGRAI
                     GRAILLAKDKKGFSIISNLLTTRALNKEEPFDAEAVLAALTQTAGDNLMRAPLRNLTR
                     RPQCDITWTLGGKLEGLIIITDDAALLDELCAATAQKENSEQDLAQRPPPPDAPPPDV
                     YGLLSPFNRPRWGALLKSGIPCVATAEVRFLEPEDRQVQRVLRAIDKKTTVEHIKESE
                     LGSPDAVLPSLDEFYALYKEVPEAIRQNEHIVEMASYSSLFDGFLFPRYKADEPGGAA
                     ALLRSEVLKGAVRRYGSPGLRSLSSGSASLGSLKSGSPDSKSLDAAVLARIDYELGII
                     DQKGFADYFLTVRDIARRASRTCGRGSAAASIVSYCLGITDVDPIRYNLYFERFLNPE
                     RQDPPDIDIDFAWDERDAVLDSVFESFGDDHVARVSNHVCFQARAAIRETARAYGIPD
                     EEISAIERRLRIESSEKLTQQDELWAKILDIANRLVGLPRNIGVHSGGVIIVPDRLSD
                     HVPLEKSGGGIKVTAWDKEGVEAAGLVKIDLLG"
     assembly_gap    18420..18519
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18520..>19707
                     /locus_tag="PLS88_04855"
     CDS             <18520..>19707
                     /locus_tag="PLS88_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOU06707.1"
                     /translation="WDKEGVEAAGLVKIDLLGNRSLAVVRDTLSNLRENGIVIDELAW
                     QPADDAETIAMLARGDTMGVFYVESPAMRLLQQKTGCGDFEHLVIHSSIIRPAANRYI
                     NEYIERLHGKPWKPVHPLLAGLFDESYGILCYQEDVSKAAIALAGFSVGEADRIRKIL
                     TKKEANRKLEAYRPAFEEGARRKGVDSATIDTVWDMMRSFTGYSFVKAHSASYAMLSF
                     KSAYLRCHYQAEFMAAVLSNRGGFYSTLAYVSECRRMGLSVLAPDINASELRCRGKEK
                     TLRLGLELIRLLASKTAEAIVSERKRRGPYRSIEDLSRRVPLDRTDAEALVGAGALDS
                     LSPELTRAKKLMYLFTQVEKCVQVVQKTDSFYTTWPSSKAPESQELFGEEVFDISISQ
                     DSRD"
     assembly_gap    19708..19807
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19808..20291
                     /locus_tag="PLS88_04860"
     CDS             <19808..20291
                     /locus_tag="PLS88_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06708.1"
                     /translation="ELFGEEVFDISISQDSRDKNSHARSFCAPYESTYESPKRILESQ
                     MKYLGTTLDIHPLVLWPKAYSKPRVKGIDLPHFVGSTVTLVGWPITAKPVLTCLEEPM
                     EFVSFEDETALYETVLFPASFQRYHHLLYEERPLLIKGRVECDHGAVVLNVFEITKIG
                     "
     gene            20564..>21132
                     /locus_tag="PLS88_04865"
     CDS             20564..>21132
                     /locus_tag="PLS88_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06709.1"
                     /translation="MKKTTWAVILGVILCIALFFSSCVTYKPVTVEIEKIPMERARIE
                     LIQPFLECLRDFGNNMARIMLTDYKFVEIQETGAGDYLYRFDTLNPKLPKYAYIVFRF
                     DPDATITTNYGTKNNIQLDNGSVGLIPADEPAEILEYSFRLRFTTTGFMEGFNPHIIG
                     PRGSYPDSLLFWFSDPLTKNEKTRRLASLL"
BASE COUNT         5474 a         4879 c         5081 g         5498 t
ORIGIN      
        1 ggatgaccta tctcataatc agcgatctaa aagaaagagc agctgcgggc gatacgacag
       61 atctctctaa ccaaaagaac tataaggttg caatagattc agccaaaaag gcttattcgc
      121 tggcaaagaa gccagcaccc aagacatggg acgcagtagc cattttatct gcgttttcca
      181 gtgagcgctc catgttcgat gcgatcgaat cgcgctatag ggaaagagta ttaaaactca
      241 agaatgtgca ctcaaatgcg gtacagttag ggctcgatct tgctggcggt atgagcgcag
      301 ttgtccaggc tgacctcaag gggttgtccg aaaaactcgg acatgagcta tctcaagccg
      361 agaaagaaga tgcgatgaag cgtgcggtgg aaattctcaa ctcgcgcatc gataagttcg
      421 gccttacaga acccgtaatt cgtcgccagg gcgaagatca gatctatatt gaaattccag
      481 gaacaccgga tccagagcgc atcaattcta taatcatggg caaggggaac ctggctttct
      541 atattgccga tttggaagct tccgcagccg tgaattccta tctttcgact aatccagtgg
      601 gcatcgatga agaaacgatg acattggata agccaggcct tgtacccgaa ggcaagatcg
      661 ttcgcaaagt ctataagaaa gacagctatg ggctcgatga atttactgga gaataccttg
      721 ttctggaagg caagccgggc ttggatggca gccatatcgt aagcgcgaca gtctcttcag
      781 atccgattac cgggcaaccc gagaccaatt tcgtgctcga caaagagggc ggagacattt
      841 tctacgagct tacgagcgcc aatgttggca agaccatggc cgttgttctg gatgaccgcg
      901 tgaaatccta tgcccgcatt caggaaccga tcagggaatc ggtacggata actggattca
      961 gcgcagagga agccgaagcg ctcgccctat tgctgaggac agctgctcta cccatcagcc
     1021 tttcggttgt gagccagcag tctgtcggcg cttctcttgg agaagacgcc attaatcagg
     1081 gacaaaccgc tatcattgtc ggtattctgt cgatcttcgt tttcatgttc ctctattata
     1141 aatgggcggg aataaatgcg accgtagcgc agatactcaa cctctacctc atgctttcgg
     1201 ttttgacagc cttcaagcta acgctaaccc ttccttctat agcgggcttt gtgctcacta
     1261 ctggaatggc agtagatgcc aatgtcatca tcttcgagcg tatcaaggaa gagttgagag
     1321 ctggcaagag cagggctgca gccatacacg ctggtttcga caaagccttc tgggcggtca
     1381 tggactcgaa catcaccacg ataatcgcgg cacttttcct tgcgcagctt ggaactggac
     1441 caattcaagg ttttgcggtt tccttggcga taggcaatat aaccagtctt tttacctccc
     1501 tttttgtgtc gaggctgatc tttgatttcg aaacagatgt cttaaagcta aagactgtgt
     1561 ctataagctg gaggatccga taatgaaaaa catcatcaaa ttcagcaaat tctttctgcc
     1621 agcggtgata atcagtttat ctattatcat tttcgggctt gtcggactct ttaccaaagg
     1681 cttgaaccta ggcgtagact tccaagcagg tataaacgag actattcagc ttgcctatcc
     1741 agctggagat gtaacctttg atggcaaagg caatgcagag ctaactgtca gcgaaagtca
     1801 gttgacgctt gttttctcag gcgccgaagc gcagaagcgt actgtagttt tcgattataa
     1861 gacatattcg acccttgcgg atataaaatc agccttggag aaagaacagg gaattagagt
     1921 gagccttgca gaagggagtg aatctcttcc ttccaatttg cttgtaccta cttatcaggg
     1981 taattcgctc ttgagtctca acccggtcaa actctaccgt gcgccaaaag atgatagcga
     2041 gcgctatgca tctatcgata ttgtacgaga agctatcagt ccgctgggag acattacagt
     2101 gcagaccata aatccgccat cttcgcagag ataccttatc cgcgttcgag atagcggcga
     2161 ggatccgcag tttatgacaa atactcctca gaccatacgc aagctcatcg aagataaggt
     2221 tggaaccgac agggtagttg tggtaaaggc agattatgtc gggcctcagt attccgaagc
     2281 acttgggaag caatcggtct ggctagtatt gatgaccctt ttgcttattc ttatctattc
     2341 ggccattcgc ttcaaaatcg agtatgcaat cggtgctgtc gtggctatag cccatgatgc
     2401 tcttgtaatg attgccttta tcgtgtggac aaggatggaa ttcaacacca caaccattgc
     2461 ggctattctg acgattctag gctattccat caacgacacc attgtgcagt tcgatcgagt
     2521 gcgagaggaa cgaaagcttc atccttcgga accttttgtc gatgtgctcg acagggcttt
     2581 gactctgact ctagggaggt ccatcataac gacccttacc actatgctgg cagtgctagc
     2641 gctctatttc tttacaagcg gaagcataaa ggattttgcg cttgcgcttt ttgtaggtat
     2701 ggtaagtggc gtatactcta caatctatat agccagcgcc attgtgctgt tctgggaaaa
     2761 caaaaagaca aagagacaag caaaagcacc aaaggacaaa ggcgctccag cagccgcggt
     2821 atccaagaat taggagtgca cgacagattt atttatgcaa aggtaatcaa gctacattcc
     2881 ttagaattgt tgacaaaagc ggggtactca ggtagtatgt gccccgcatt tggcgccgta
     2941 cccaagtggt aagggagagg tctgcaaaac ctttatacat cggttcgatt ccgatcggcg
     3001 cctttgtttt cggttgcgat ttcataatta gcggcgattt tatatatcgc tacgcctcta
     3061 aagcattttt acaataattc catagaatac aaagtaaaaa ttgaaatatc gcttggacaa
     3121 tcttgacaat ataggagttc ccgttataat aattaactaa attatttggg ctcttttgag
     3181 ccaaggagaa agtttatgaa aaagaaagtg tttctgtcgg tactggcagc actgttgatc
     3241 agtgtgccgg tattcgccgc cactcaggtt gagacgacaa ttcaggcaac aatcggagct
     3301 gatctaagca ttactaccaa tattccaggc acaaaggcaa tcgatattac ggctagctcc
     3361 accacgcttg gttcagtcac catttcatcg aatctgacga actggaagat cgttattcat
     3421 tcagctaagg gtggcaaaat ggtcagagta ggtggttcag atgtataccc ttacctcctc
     3481 aattttggtt ccactacagg gatcgatcta gctagtgact atgaaattgt aaaaacagcc
     3541 cctcagtctc cgacatcaac gactgtagtt gttacatata ccaaggcttc ggagcttaat
     3601 cctgcccttc ctgcaggtac ctacgaggat accttaacaa tttcgcttgt agcgctttga
     3661 gatccaccat cgcgctgctg ataaaaaagg ctgcccgctt attgggcggc cttttttatg
     3721 atattcttat atgatcgaat tatgagccct attagcagcc agaaaaagaa taaggccaag
     3781 atggcaaaag tcttgcttcc gctcttcttt tgtatattgg cgatattttg ttttttcatg
     3841 ctatggaaga gcgcaaatcg atcggaatca gagctgcttg gagctgtctc caagagaata
     3901 gctctgcaaa tctctgagcc attgcaggag atgcttgctg ttggcgataa atttcgcgag
     3961 ctgcttctaa ctggctcagg taaaacctat gcagcgctaa gaccgcttgc cgaagagact
     4021 cttgctaact taccttatat agactcaatt acgatagctc caggtgcaat agtcagatat
     4081 tttttccccg aaggtagagc atcctcttct attggtcacg atttgctcga taatcctgaa
     4141 aggatggatg ctctggtcga ggctgtacgc agaaaaagcg ccgtgttgca agggccggaa
     4201 ctatccgcgg aaggaaaaaa tctcgcattc ttgcgaatac cagtgattga aggttccgaa
     4261 ctttggggtt tcgtgagtat cgcattcaat gcagacaaat tattgtcaga tctggattta
     4321 gtttcgcaat tcccagggct atccattgcc attgcatctt ccaatatgga agggggtgac
     4381 agaaggcttt tttggggcga agagcgtgcg cttaccggtt attctgctgt tctgaaaatc
     4441 ggatcggaag actttccatg gactatatat gtcgcatcga tgtacccata taagcgaaca
     4501 gggctatggg gagtgggact tcttatactt gtactactga gctgtgggct cgtcatacgg
     4561 gcggaatttt tggaaaagga gagcagcaag gctccagtac gcccaaaaga aacctcaata
     4621 gccataaaac ccttcgtacc aaaaactgaa tcaatgcaag agccttcaat agagcttgtc
     4681 aaaaaggtcg atattcctaa ggcgtcagcg atggaagagt cgatggcaca agagatagtg
     4741 gcagaaaagc tggcgtcaaa agaatcgata gcacaagagc cagcatcaga aaagctggtc
     4801 agtccttcat tagaaaagcc aataaaagag ccagctaacg ggtctccaaa ggatatggct
     4861 gtcttccaaa atcaagctat tgaaaatgaa gccgaaaaga aagaagtcgc cgtgtctatg
     4921 gaagagattc caggttccat atcagtgctc gtagttgacg atagcgaagt gaatcgcgat
     4981 cttcttttgc gaatgctcac attaaaggat tacaaagcaa aagcggtatc gagcggagaa
     5041 gtagctctaa aagcgctctg cgaagagagc ttcgatgtat tgctcatcga ctgtatcatg
     5101 cctgagatgg atggctatgc tcttgcggga aagatacgta ccgaaggaat ttcgagcatg
     5161 cctgtgctaa tcgctatgag cccgcggcat gaccaggaag aagctgagaa atgtgctaat
     5221 gccggcttcg acaatctgct cgtaaagccc tttacgatga cagccctaga ccagcaaatt
     5281 cgcctggctc ttggcaatag aaatgcagaa taaaagcttg cttgctaata gcttgcatat
     5341 agatcgaaaa tagagcaacc tcggcgtcgt catttcgaaa taaaaattta gtttatagag
     5401 atattagaca taaggggcaa aattctccat tttatcgaat agctttaatt tacaatatac
     5461 acattgaata gctaattcat ggcctaataa aaagatataa gtattacttg aaagagccta
     5521 agaatcctaa attaaaaaag atagagaaaa tctaattgta caaaaaattt atgtcgatta
     5581 tatttatatc aaagtgtagt ggtggaatcc gttggaggaa atcacaaaaa acacaaaatg
     5641 tatccgtggg acatagatcc tgcgggcagg agacgcaaaa tgaaaaagta tgcgttggta
     5701 gctctgtttg cggtagtgtc gttagggatg gtgtttgctg tagtagcaaa tccaggtagt
     5761 ggtactgtat cgatccaagg cagtgtcgac cagtatttct cgctgactgt gcctgcgagc
     5821 tatacgggta gtatctcgta tgatagttca acccattggg atttatctga tatgaaagtg
     5881 atttccaatg tgaagaattg ggtacttaag gccagcagtc agaataatgg tgttttaaaa
     5941 gacacggcca atggtgcgga tatcgcttat aaagttactg ttggtagtct atttactaaa
     6001 caacagctaa caagcggggg tatcacgtct ggtccacagt cgaagacacc aaaagcggga
     6061 acagcttata agtttagtat cgacattgat gccccgagcg aactttatca ggctggtact
     6121 tacagcgatg ttatcacctt gacgatcagc aatcagtaat ccgtaacaga gtgattacag
     6181 gaaggccgtc cgggcaaaac cgggcggcct ttcattttta ggtagctatt cttgcttttt
     6241 gtccatgttc ttacttttgt ggcttgtcta tagcgccgtt cagcaactct gtagtccaag
     6301 tccttgttcg ctttcctatt tttctatact tttaacttat gaatcgaaat tctgtgctgc
     6361 gccgtccttt caagtttaaa tacttcaatg cgacattgta tttgatcgca gtcaatgtgc
     6421 ttatctttgc tctgcaatat atttggccct tacttacata tttgcttgct ctaaaccctg
     6481 gtgccgttgc ttcgggctgg gtatggcagc tctttaccta catgtttgcc catgcgaatc
     6541 tgacgcacct catagtcaat atgttggggc tgtatttctt cggtaggcct gtggagcata
     6601 gtcttggcag ctatgaattc ttgctctatt accttggctc gggactgttt gcaggcattg
     6661 cgtcttttgc tatctatttg ctttcgggag catggaatac atatctcctt ggcgcttcgg
     6721 gggctatttt ttcactgttg ctcgcttttg cggtgctata cccgagggcg atggtatatg
     6781 tttatggcat aattcctcta agagctcctg tcatggtgat aggatataca ttgatcgaga
     6841 tattttcgtc tttcttgggt gcggcaagct ccgtagcaca tcttactcat ctggcgggtt
     6901 ttgttgctgg agcagcttat ttccctattc gcttaggggt aaatccctta aaaaggctca
     6961 gcgatcgcta aacctgcgat tctctacttc ataatgcatg tcttcgagaa ggtccatgac
     7021 ggtatctaca atggcttgtt ttttatcctc aaggaatcga agatcatatc gcaccgaatt
     7081 caggaattcc ttgttatcgt ggacagggct tacattgtag attatgcggt ctctctgaag
     7141 aatatcgtct tccatggctc cagaagggtt gagtcttaga atgttgccat tgatcgacac
     7201 gaggcagaat ttagaaaaag atttgatgta ggaggctatt tcgcgaacgc cgcgtttaga
     7261 agaagggtcc catggccgat aacgcgttcc tgcagggaaa actagaatca ttttgctatt
     7321 gtgtttagcg ccctcgaggg ctctcattgc tgcgcgattt atcgagatac ttcgcttcat
     7381 ttcttggtat agctcgatcg ggtctgtgag tttttctctt attatctgca ttgaacgtga
     7441 aggatagatg actattctgg tatatgctcg ggtaaacgca tttacagctg gattttcctc
     7501 gttcagcttg atacccgcaa tcgctactat atctgaagcg agcttcttgc cttcctctcc
     7561 ttcctgtcgc aaaagataat gaaatacagg caggtcgaag ttcgaataat gctccattag
     7621 gagtaatgta gattcgccat ttttagcatg ttcgtatagg cttttgaggt tatccagacc
     7681 ttcgatcttc gaaccatctt ccagatggtc cgcgattatc gcatcgataa aagggagtat
     7741 ttggcctatg ccttcttgat atacatttag ctccgagata cgagcttctt tggggacacg
     7801 ggcaataatc tcgcccacta gaggagcata tttgctcttg attgtgtcca tggcatcctt
     7861 gattatagac tataggtgtt ttggtaaagg gtgtcaattt atcttggtca taaccttatt
     7921 tggccaggaa cagaatatta tgcaggcata gacatggcaa tcgttgtaag cgccagcagg
     7981 cgcacggata tagcttcttt ctataccgat tggtttttcg agcgattggg cgaaggcttt
     8041 gtcgatgtgg tcaatccttg gaatcctcgg cagattcgac acctcagcct tagacccgaa
     8101 gatgtggctt gttttgtatt ctggacaaga gacgcaagtc caatgctcag aaacttgagt
     8161 atgctcgaaa aatatgcgtt ttattttcag gtaacggtta atggttatgg tgttccgatg
     8221 gaaccatctg gtcccggagt cgaggcagga atctcggcac tgaaagcact atcctcagag
     8281 ctgggctcga ggcgagtggt gtggagatat gatccagttc tgcttacaga gcaatataat
     8341 tcagcttggc acgtagaaaa cttcaaaaga ttggcagggg agatcggaga aagagtgcgt
     8401 cattgtgtat tgagttttta tgatagctat atgcattctg acaaaaggct ttcgaaggct
     8461 ggatatagag tagcgccagt agatcgggct tcgaatgagg tcagagatct catgtttgcg
     8521 ctggttggta tcgctcgtga aaatggcctt tccttatcgc tatgtgcgga gcctgagctc
     8581 aaagagatgc tcggcctcga gcagtatgct tgcatagacg catgcataat tggcgagctt
     8641 tctagattac ctttttcatc gaagagagac aaaaaccaga gaaaaggctg tcagtgtatc
     8701 gaaagcgtcg acttagggag ctatggcact tgcccaagag gttgtctgta ttgttatgcc
     8761 aatagatgta aggccaatat aagccaatag caaccaataa aaatactttt tcaataagga
     8821 acgaatagca gatttaaaag ctaagagaat tagctaacag accgtcgctt gagaaaaata
     8881 tcagccaatt cggcaaaacc agcgccagac tctctttgag tcacaaagtg aggaggagtc
     8941 ttcattagtt ctctgtattt gaggacattt gccatggcac aggccatcgg aaaatgctcg
     9001 aacatgggtt catcatttgg agagtctcca gcaaagataa ctgtgccgac atcgcgactg
     9061 tcatcatagc cgaaacgagc attcaagaaa gaaagcgcca tagatagctt gtcgtaatcg
     9121 cccatccatg cattgacgtg aatcgaagat atcttagccc ttgctccacc ttctaaaaag
     9181 atcgattgaa tgcgcaatgc ttctgatagc ggaagtattg gctcttcttc ggcaaaatcg
     9241 atcgcgatat catagaggcg cgagaattga tcgcgtgcta gccttgcgcc tggtatttcg
     9301 gaaatcacgc ggtcgcgcag cgccaagagg cgtggatcgg tatttagaat agcatttgga
     9361 tgcgaaagcg taagaagatg atgcccttcg ccttcccaaa aggcaaaagc gccattttcg
     9421 cccaccaccc catctgctgg ccattcgcgc gcaatgagat cgcaccagcc cgcaggccta
     9481 cctgttactg gaataacctt tagtccagcc tcgtgaagtc gccatagcgc agcataactc
     9541 tctgcgagga gctttccatc tgtggtgagt gtgtcgtcga tgtccattag aacatatcga
     9601 atctttcgcg cttcttggct ggttagagaa gttataggca taagactaga gctatgaaaa
     9661 ttatccatat caagctttgg tacaataatg ataaaaacat cataaacaag ggcttgtcct
     9721 gcgtcaagaa aagtatggta aacttaagca tggttattat aagattcttt tatcagtgcc
     9781 tctgcaacga ctgctttcgc aaaatacctg gacatctcaa gaatctctgc aattggctca
     9841 atcataaact tgataataag cgctatgcta atcggcctgc tataattgca ctattattct
     9901 tttgtacttt tgttcgggtt catgcacagc ttattccatc gatggagcct gtactccatt
     9961 taaaaaccga ttctttcgac atttattcac cgaagagcct ggaagccgag gcaaaaaggc
    10021 tagcaagctt tgctgataag acctatggag aactatgcga ttttctggga ctggagctaa
    10081 gaacagagcg cattcctgtg cttttgagcg atcttcaata ttctttgaat gggttttcta
    10141 ctctttatcc ttctaatagg attgtgctcc ttctggcgtc tgctgatccg aggagccagc
    10201 ttgctacttt ggaagacgag ctatattcag tgtttttgca cgaacttgtg cattatatca
    10261 ctctgaatga gcgaagtctt gcttggcgag ttctgtcatg gttcggaggg gattgggttg
    10321 ctccagaaat atatatgatg ccacaggcta tggtcgaagg aaccgccgta tgggtggaga
    10381 gcaggttcaa ggagaatggc tctgggaggt tgaatgatcc agcagcatta gaatttgtaa
    10441 ggctagagag ggcacgtgga aaagatcgat ccctttggga tgtttctggc ttggaagatt
    10501 tctatggggc aggctcactg ccatatctat atggtggtct ttttgtagat tatttaagcg
    10561 agcgatttgg gccatccatt gtcgggcggc tctggcaaag ctcgggtcgt ggaattatct
    10621 atcgaggatt cgatggcgga attctctcaa aaggaattct ggaacgcgag acaggagtaa
    10681 aaccaaataa gttgtggcag gattttttag tctggattga tgaaggaagc gagttagagg
    10741 tgcaagaagg gcagggcgga gtcgatccaa ttgagatgaa tgatgctgcc gatatgcctg
    10801 ctgagaagga gcttttttct ggatatgttg gagcccttgg ggctggtggc gggaaagtct
    10861 attttgtgga tttggaacgc cgcggtatgt atcagctctc ggtagtcgat ttgcaggagg
    10921 attctaaaaa taaatcggag ttcgaaaaaa aagctatggc aaagaagcca gagaggcttt
    10981 ttgcaactga ccaaatgtta cgtaacattt attgtaacgt aaaattcgga aggctagaac
    11041 tcgactggac tcgaatcaat tcgcaaaacc aggctattcc cgctcgctat atatacgacc
    11101 tatccgagca caaactccgc tacgaaaaag accttccaca ggctagtatt ggcgaggtgc
    11161 tattcgaaaa gcattcgggt acgaaagaag aaatctctct ttatgattcc tggcaggatc
    11221 ctcttacgtc tatcgaatat ggccttgtaa ggcttggcac tgcggttctc cctgctcgaa
    11281 gattcgcgaa tgggcagata gaggtagccg atattcccga tttggccata cgatggcttt
    11341 cccaaggatt tagggagaat tccgagccat cggattcgat tagctttgct ctaagccttg
    11401 tccctgacaa cggattatcg cgccttgcta ttctggagca aaataatggg ctttggcgat
    11461 ttgcaatagc cagaaaaagt ccttcaggcg gtgttcatat gcccgtcttc acagacgcct
    11521 ctcaccttgt ctatcgagca tccataggaa acgggcaatc gcgtcttctt cttctcgata
    11581 tttcggattt caaagacgag atcgaagctc ctggtcaaca ttttgattct gagcccctta
    11641 ggtggatttc gcctacggca tggatagaga gctattctct ggagatgagg aaccagtcag
    11701 cccatctaga gagcagtgaa gaagtacagc atcagctaga gcctaacaga gaggtacagc
    11761 tccagccaga gactagcaaa gtaccccaac ctgagccaga gctttcgtca gagctttccc
    11821 tagagcattc caaagcagta caaaagcgat ctacgctctt tccagctatc ttcgagacaa
    11881 gtcgaatccc ctatgccgat ggcacattgc tgggtataga tttcatagcg acagatctta
    11941 ccgaacgcct ttcctggtca gctcttgttg gatgggattt cctcatagca aaaccagcta
    12001 caagcattgc tttccagctg agtgccgggg cttggcgatt cgagctcagc gcttgggacc
    12061 aaggagtctt tgcctttcct gttgcgcgca tatctgctct tgaaggagcg ctgcactggg
    12121 gacatgccct tatacctaca tttaagagta tttctacaga gatacatgct cgatacggag
    12181 gtgtgcagaa taaatattca gaacagggtt tattctatct agtgccagat tattgcgcat
    12241 gggtagcggg ggcaggagtc gaattcgata gcctttcttc ttcttataag ccaccctaca
    12301 atccttcagg tatttctctc aagagcggaa tcgaatatga aggagctaat tcatcgaatt
    12361 ttggaggtct ttctatatcg ggatcgatct atttgggaga atcgacaatt gcggagcgaa
    12421 taggagcttc catttatgga gcctggactc ccaccggagg agtggctttt tcgccagcag
    12481 ctcgatatct ggaaaatgca ggaaactatg agttctcagt agcagatatt ccatatcctg
    12541 catataagga atatgaagct tgctcagatc tttcttcttg gtacctctat ggagagataa
    12601 aggcaaggct cttttcttta gagctaaact ggattatgag ccatctcttt gcgccttctt
    12661 ttgcactcag aagaatatcg atgagttcag gattgcgcgg tgctggcctt gagatcgaca
    12721 atacgccttc gcttttgagt tcggcttttc tccaggctga tttcgactgt gctttgcttt
    12781 ccggccttgc ggccgtgacc cacatgcatc tcactctgga agcggcatgg gccttccaag
    12841 ctgacaaggc agatgtgaag ccatttcatt tttctgtagg gctagccgca ggactataat
    12901 aatggcgaca gagctggatt actcctttag ctattctagg gatggcttta gatgatcatc
    12961 gagtataatt aggccatcat ttcatatatc gcttggatat cggaatcctt gtagccgggt
    13021 atattccata acttactcaa agaaagagcc tgttttgtac atgcttcgat atcgagcttt
    13081 tcgattcctg cttcatggag ccggatcgga gtatgaatat gccgatacca gccctccagc
    13141 tcttcgatga cgaggtcagc tgcatcgaga gctgccttgt ttctcagaga ctctcccatg
    13201 cctagtattc gttctccgaa tagcacaatt cgatgcgcaa tgtgcggttt catgtatttt
    13261 aaccatgccg gaataacgat tgaaagacct gccccatgcg gcagatcata gagcccgctc
    13321 aaagggtgtt ctagcatgtg atttggagtc gaagcgcctt ctacgcccgc aacaaaaaga
    13381 ccattccagg caagcgctcc cgcccacatt atggtagaac gagccttgag atctctaggt
    13441 tgtatcatca ctgtatcaag agcacctatt acagccctac aggtcccttc aacaattcca
    13501 tcttgaagag gagaatcttc gcttgctgta aagtagccct ccatcatgtg cgaaaggatg
    13561 tctgtgcagg cataagccgt gtattggatt ggtatgtcca atgtgagcga tggatcgaga
    13621 aacgatagtt ttgggaaaac cagttcggaa ctaaaagaga atttctcgcc agtcgcgtca
    13681 ttggtcataa ccgaggtagg attcagctcc gaagaggtag ctggaagggt ttgaaccgca
    13741 attataggca atgcattctt gataatagcc ttgcccagat aaaagtccca aagcggctcc
    13801 tcgttgcagg aagcgacagc tatcgacttt gcctcatcga tcacactacc gccaccaacc
    13861 gcgataatca tctcaagctc atgttctttt accttttgtg caccttcaag ggcatgcgag
    13921 ataatcgggt tcggttgtac gccgccatgt tccatgaatt caatatcagc gccgcgcaac
    13981 tgagctacca ctttctcata gaggccgctg cgcttgatgc tctgttttcc gtaaagaaaa
    14041 agtgctcgct ttccaattcg cgatgcctct ctgcccagct gatcgagaat tttcgtgcca
    14101 aagatcacct ttgtcggtgt gtaccacgtg aagtttttca tagtaacctc caccgatatt
    14161 atacggcatt agttgcatgg atacgatagc attgttctcc gagagtggca gatgaagctt
    14221 ctttgctttt taagtaattc cttatttttt atcgattttc acaggatgcg ctacaagatc
    14281 cccagcagct ttgtgcaaaa gtgatcaagc taagctacat ttcttagagc taagtaatct
    14341 ttatctcggg gtgcacgaca attttgtgca aaggtagcta agcagcattt cttcgatttc
    14401 tcttgatacc tacacatctg tatagtataa tttaagcatg gaggcaatgt tttccccgcc
    14461 aagcgatctt ggcgcttacg atctcagaat tgggacagtt gggtatgaat atcccgaatg
    14521 ggagggcgtt ctctatagtc atgggacgga taaagaggca tatttgcgct tatattgcga
    14581 gcgcttttct accctagagc tggattttac attccatacc atgcccaagg ccgcccaaat
    14641 gcgagatatt ctcgctcgag ctctagggtc gtctgccgga caggcgcagt ttcagaacca
    14701 actaattcag aagctgcctg ttcaggagcc ttctggtagg gcgcctacgg atttctcagt
    14761 tattgcccac agctctctca ctcatcggat agatccattt acctggtcta gcagtctgag
    14821 gacatttctt aagggcatag agccactggc agaagcggat cgactctgtg cggtgttact
    14881 tgtttttcct ttttcatttc gctatcgcga agaagaacgg cgttatctcg ataaattgct
    14941 aaaggctcta gaaacttatc ctcttgcagc ctattccctc gcggtcgaat tccagaattc
    15001 cgagtggttg acgctccgcg tcatagagga acttaagaac agaaatgttg ctctctgtgc
    15061 gctcgatatg ccaaggatgg aaggccgacc gccgatttct gatattgtca cctcagatct
    15121 cgcatacatt cgttttctcg gaagagctgc acctggagtc cttacggatt cttcgaactc
    15181 ttcttcaata gaagaaggag cgcggaatac agaagaagga ttatggtgtc cagaccttta
    15241 tcgttataaa agcgaggagc ttgcgctgtg gattcctcgc attctctcta tgagcgcaca
    15301 agcgtccaaa atgcgcatat tttttgggaa tcggagaaag gggaatgccc ctctcgatgc
    15361 gtgtgcgttc accaagcttt tgcgtgcggc agggctttaa gggtttataa ggtgctataa
    15421 agggctataa ggaaacgtag atgtctacgg gaacgattct gcatatcaac gcggtaggat
    15481 ttcctgccgc catagagatg aatctcgatc ggtcactcaa ggcttggcca tttgtcgttg
    15541 caaatgagcg ggcgccccgt gctgtcgtca ttgcaccctc gccaagcgct catcgggaag
    15601 gtgtacggcc cggcatggtg ctctcgatgg ccaaaagcat ctgccccaaa ctcgctgtgc
    15661 gacctccaca gcccgagctc tatcgtaaaa tcgaagatac tctgcagaag ctctgcctat
    15721 cctttactcc ccttgtcgag cacgcgggca cggggcatct ctatctcgac cttgcaggga
    15781 caagccgtct tttcggtgct cccgaagatg cctctcagaa gctaagggcg cgcatatatg
    15841 agcaaacagg gcttactcca acattgggtc tttcttcgaa taagaccgtt agcaaggtgg
    15901 cgacaagagt ctttaggccg tcggggtttg tcgctctctc cgccaacgaa gaaagtcaac
    15961 ttttacgcca tcaacccgct gcgattctgc ctgggatagg ccctgtactt cttgggcgtt
    16021 tgtcgctgct agacatagag gagataggcc aaatagccga cctaactgcg ggcgaagccc
    16081 gtactttggg tccgcgagga ctcgagctcg tcatgcgcgc ccgcggaatc gacagttcgc
    16141 ctgtagatcc ggaacctgtc gagcgcaaga ccgtcgacga cacaatcgtt tttgaaccag
    16201 acacaagcga tcccgagatc atgaaaattc gtctgagatc ccttgttgcc gagctgggct
    16261 tcgctctcag aaaggcgaat gtaggcacac acaaagcctc cgtggcgatc tcgtacaccg
    16321 atgggccgac tgcgcgcgca acggcgagga caagccaact tcttgtgcgc gacgatgaaa
    16381 tattttcgct cgcggtcctg gctctcgagc gggcaaagaa acgccgtgta cgcgtgcgcc
    16441 gactcgaact cattctatcc gagatcgatg cggcaggtgc aaatctggat ctcttcgagc
    16501 cagaagaaat tcgccttttt cgcttccaga gcgcgctcga caaggtccac agcagattcg
    16561 gattcgaggc tcttgtgcca tgttcggcgt tgcttgttgc caattcctta gggggccagt
    16621 catgattgcg cccctctttg tcaactccat gtattctctg ctctatggca ctgcttcgcc
    16681 tgaggcgctc gcaaagaagg ctgcagccct tggctacggc gcactcgcgc tcaccgatcg
    16741 aaattctgtc tacggattac cagccttcat cgaagcctgt aaacaggaaa gattacgccc
    16801 aattctgggc accgagcttg tctatagaga gactagacag gaaaacacaa aagaagccgc
    16861 ccaagaagcc agaaaagagt acaaacaaga agtcaagaaa gaaattatgc aagaaggcag
    16921 ggccatcggc agagccatcc tccttgcaaa ggacaaaaaa ggcttttcta tcatctcgaa
    16981 tctgctgacc acccgcgcgc tgaacaagga agagcctttc gatgctgagg ccgtccttgc
    17041 ggcgcttaca cagacggcgg gagataacct catgcgagca cccttgcgca acctcacacg
    17101 gaggcctcaa tgcgacataa cgtggacgct tgggggcaag ctcgaggggc taatcatcat
    17161 aaccgacgat gcagcgctgc tcgatgagct ttgcgctgca acggcgcaaa aagaaaattc
    17221 agagcaagat ctagctcagc gcccaccacc tccagatgcg ccacctccag atgtctatgg
    17281 ccttctttcg cctttcaatc gcccgcgctg gggggctctt cttaaatctg gcataccctg
    17341 tgttgcgacg gcggaggtgc ggttcctcga gccagaggat cggcaggtac agcgcgtgct
    17401 ccgcgccatc gacaaaaaaa ctacggtaga acatatcaag gaaagcgaac tgggttcacc
    17461 ggacgctgtg cttccttcct tagacgagtt ttatgctctc tataaggaag ttcccgaggc
    17521 tatccgccaa aacgagcaca tcgtcgagat ggcctcgtat agcagtctct tcgatggttt
    17581 tctctttcct cgttataagg cagatgagcc gggtggtgca gcggcacttt tgcgcagtga
    17641 ggttctcaaa ggagcagtgc ggcgatacgg atcgcccggc ctcagatcgc ttagttctgg
    17701 atcagccagc ctcggatccc tcaagtccgg atctcccgac tccaaatcgc tcgatgcggc
    17761 tgtgctagcg cgcatcgact acgaattagg catcatcgac cagaagggct ttgccgatta
    17821 ttttctaaca gtgcgagata ttgcaaggcg ggcatcgcgc acctgtggca gagggagcgc
    17881 cgctgcgagc atagtgtcct attgccttgg aattaccgat gtcgatccga tccgctacaa
    17941 tctctacttc gagcgattct tgaaccccga acgccaagat cctcccgata tcgatatcga
    18001 tttcgcatgg gacgagcgcg atgccgttct cgactctgtc ttcgaaagtt ttggcgatga
    18061 ccatgtcgca agagtatcga atcatgtctg ctttcaagcg cgagcggcta tccgcgagac
    18121 ggctcgggct tatggcatcc ccgatgaaga aatatccgca atcgagcgtc gccttagaat
    18181 agaatcgagc gagaagctca cacaacagga tgagctgtgg gctaaaattt tggatatcgc
    18241 aaaccgcctt gtgggtctgc cacgcaatat cggtgtgcat tctggaggtg taatcatagt
    18301 gcccgacagg ctctccgatc atgtgccact cgaaaaatcc ggaggaggaa tcaaggtcac
    18361 cgcctgggac aaagaaggcg tcgaggctgc tggccttgtt aaaatcgacc tcctcggtan
    18421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gggacaaaga aggcgtcgag
    18541 gctgctggcc ttgttaaaat cgacctcctc ggtaatcgct ctctcgccgt ggtgcgagat
    18601 accctctcca acctgcgcga aaacggcatc gtaatcgacg aactcgcctg gcagcccgcc
    18661 gacgatgcag aaacaatagc catgctcgct agaggagata ccatgggcgt cttctatgtc
    18721 gagagccccg ccatgcgcct attacaacag aaaacaggct gtggggattt cgagcatctt
    18781 gtcatccact catcgattat tcgacctgcg gcgaatcgat atatcaacga atacatcgaa
    18841 cgactccatg gaaagccatg gaaacccgtc catcctcttc ttgccggact cttcgatgag
    18901 agctacggaa ttctctgcta tcaggaagat gtgtcgaagg ccgctattgc gttggcgggc
    18961 ttcagcgtgg gagaagccga cagaatacgc aaaattctca ccaagaaaga agcaaaccgt
    19021 aagctcgaag catatcgacc agccttcgag gaaggcgctc gtagaaaagg agtcgacagc
    19081 gctaccatcg atacagtctg ggacatgatg cgttccttta caggctacag ctttgtgaag
    19141 gcacattccg caagctacgc tatgctgtcc ttcaagtctg cttatctgag atgccattat
    19201 caagcggaat tcatggctgc agttctgagt aatcgaggcg gattctattc gaccctggct
    19261 tatgtgagcg aatgtaggag gatgggcctt agcgtacttg caccagatat caacgcaagc
    19321 gagcttcgct gtcgaggcaa ggagaaaacc ttgcgcttag gcctcgaact cattcgcttg
    19381 cttgcttcca agacagccga ggccatagtt agcgagagaa agcgtagagg accctataga
    19441 tccatagaag accttagccg tcgtgttcct ctggatagaa ccgacgccga agcccttgtt
    19501 ggagctggcg cgctcgattc actctctccg gaacttacgc gcgccaagaa gctcatgtat
    19561 cttttcaccc aggtcgagaa atgtgtccag gtagtccaga agaccgacag cttttataca
    19621 acctggccgt cttccaaagc gcctgaatca caggaactat tcggcgaaga agtattcgac
    19681 ataagcatca gtcaagattc gcgcgatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19801 nnnnnnngga actattcggc gaagaagtat tcgacataag catcagtcaa gattcgcgcg
    19861 ataaaaatag ccatgctcga agcttctgcg ctccatacga gtcaacctat gagtcaccca
    19921 aaagaatcct cgaatcccag atgaaatacc taggcacgac cctcgacatc cacccccttg
    19981 tactctggcc caaggcctac tctaaaccgc gagtcaaggg catcgaccta ccgcacttcg
    20041 tgggcagcac cgtcacattg gtaggctggc ccatcacagc aaaaccagtc ctcacctgcc
    20101 tggaagaacc gatggagttc gttagcttcg aagatgaaac ggccctctat gagaccgtcc
    20161 tctttcccgc gagctttcag cgctatcatc atctgctcta tgaagagcgt ccccttctca
    20221 taaaaggcag ggtagaatgc gaccacggcg ctgtcgtctt gaatgtgttc gagataacga
    20281 agattgggtg aattagaaag atgggcgata gatttctcta ttcattcgac actaataatc
    20341 tctggggaaa ccttgacacc agctatctcg catcctagac tgaaattgcg caaactcgaa
    20401 ttttacaatt ttgaaatgtg caattatcaa atgcgcaatg cgcaatcttg aattgcgcaa
    20461 tcttgaaaca tcgataaata aaaacggtca tatgaaagat aaaacaatga aatacaagag
    20521 ctagagaacc aattggcgcc ttgattttca ggagggggtt cccatgaaaa agacaacatg
    20581 ggctgttata cttggagtaa ttttatgtat agcgcttttt tttagttcct gcgtcaccta
    20641 caagccagtg actgtcgaaa tcgagaaaat accgatggaa cgagccagaa tagaactcat
    20701 ccagcccttc ctggaatgcc tccgcgattt tggcaacaat atggcccgaa tcatgctcac
    20761 cgactataag ttcgtcgaaa tacaagaaac aggcgcggga gattacctct acagattcga
    20821 cactctaaac ccaaagctcc ccaaatatgc ctacatagta tttcgtttcg atccagacgc
    20881 aaccatcacc accaattatg ggaccaaaaa caacattcag ctcgacaatg gttctgtagg
    20941 tctcatacct gccgacgaac cagcggaaat cctggaatac tcctttagat tgagatttac
    21001 gacgacaggc ttcatggaag gattcaatcc ccacattata ggcccaagag gcagctatcc
    21061 agattccctt ttattttggt tctccgatcc tctgaccaaa aacgaaaaga ctcgtaggct
    21121 ggcctcgctt ct
//
LOCUS       DAOSOB010000032        20219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_2658_length_20219_cov_97.879339, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000032 DAOSOB010000000
VERSION     DAOSOB010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 20219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20219
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2658_length_20219_cov_97.879339"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PLS88_04870"
     CDS             complement(<1..728)
                     /locus_tag="PLS88_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU06710.1"
                     /translation="MEKTKQQPETAHNSSELSAIMAISELSIMDNNGCSDEYIRSLGE
                     KTAHALERLGGIGAAASAFHFCPSCGSPKLYSVRSRMWKCPTCGFEYFHNVATAAGII
                     IEAKNKIVMIRRHKDPMKGRFALPGGFTEPGERAEDAALRECYEEIGWSPTHINFLAT
                     FPNVYQYQGIPYATCDVYFYSRGNIVEIDDFHLDPEESDSVEFVTAGAIPWADIAFES
                     TIRALHYFILHESIGTPPHLPEFGA"
     gene            756..2663
                     /gene="uvrC"
                     /locus_tag="PLS88_04875"
     CDS             756..2663
                     /gene="uvrC"
                     /locus_tag="PLS88_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOU06711.1"
                     /translation="MNAKYAKKCKSPEAKRAPDRDKIKGVEESSKETIVETDAERRAR
                     LKALVKQAPECPGVYLMRDDTSEVIYVGKAKVLKNRLSSYFAGKKDTKTRHLVSHINN
                     IEWVLAAHEYEALLLENNFIKEYTPRYNINLKDGKTYPSIRITNEEFPRAFRTRRIIN
                     DGSEYFGPYPSAETIDTYLDLIKRMFPLRRCVNMKKHTSPCMYYHIGRCPAPCADRIS
                     REEYMEQVDKVRKLLSGDTESLVKDLEREMSRAAEQLRFEEAARLREAIRAVQSFEGR
                     SNVVDFNSLDARDYIAWEANGDEVSYVIFQMREGKLKNRDSFIEHCIDELEALDSFLA
                     HYYDSGREPPPQIFVRRADNTATIARYLSDRAGAPVEIKIPDSERHWAVMNMAVQNAK
                     EELAKRRRAHGDIQALVELKERLGLMAVPMIIEGFDIAQLGGKHTVASLVSFLGGVAN
                     KKNYRYFKIRSVEGKVDDFAAIREAVARRYTRLINEEAELPDLVLVDGGAGQVSAAKE
                     ILDDLGLDIDVIGLAKKNEEIWLPDKSDPLILPKDSPALRILVAVRDETHRFATGLSR
                     KLRSGDATFHLLQEVPGIGDTRARKILNTLGSLEAVAEAEPGYISSIARVNEDTARKV
                     IELAQRTVGLG"
     gene            2939..4942
                     /gene="tkt"
                     /locus_tag="PLS88_04880"
     CDS             2939..4942
                     /gene="tkt"
                     /locus_tag="PLS88_04880"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOU06712.1"
                     /translation="MNTSALQAIALSVRSLTIDAIQKANSGHPGMPMGAAELGAYLYG
                     VELKYYPKDPKWLNRDRFILSAGHGSMFLYSLLHMAGFDLSLDDIKAFRQVGSKCPGH
                     PEYGRTPGVETTTGPLGQGIATAVGMAIAERKMSAHFNKPGQEIFDHFVFVLAGDGCM
                     MEGVASEACSLAGHLGLGNLIVYYDSNHISIDGPTDITFSEDVAKRFEAYGWQVLRGS
                     MYSFEDIASLTARAKAEKDRPSLIVLDSVIGKGAPTKQGTSSAHGSPLGEEEALKAKE
                     ALGIPIDKPFWVAPEAYSYFEEHRKELEKDYAAWQLRFEAWKKANPSLAQELAIWLSG
                     KEMNKLDLPSFVVGEKVATRNASGKCLAAISEAWPNFIGGSADLTSPNVSQLPQNADG
                     TPLVFSKSNPNGRYIYFGVREHAMAAIANGIALYGGLRPFIATFLVFSDYLRPALRLS
                     ALMGLPVIYVLTHDSIYVGEDGPTHQPIEALAALRAIPNLRVLRPVDAEETAVAWRVA
                     MERLDGPTVLVLSRQNLPVLEKYESDWPSKIDLGAYVVKDSDRAPEVTLVGSGSEVSL
                     AISAAEIVKSKRQETAIRIVSVPDRRAFYAACPLVREAILSPGSLVFVAEAGVAQGWE
                     RIAPADQILSIERFGESGPGDKVAEHLGFTAQVLAQMILAKLK"
     gene            5209..7302
                     /locus_tag="PLS88_04885"
     CDS             5209..7302
                     /locus_tag="PLS88_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU06713.1"
                     /translation="MVRSNAKRHSILAHLRELRPIERFWFVIFFLILLGSLVIGAYYQ
                     WQNILHDKKLYIELIGKSKISQIELWYTAHITKAEELPNSLAFLNLVSEAITDPSKEN
                     LERLDEYLRPSLSSYNYADTAVLTSNLDVLYTLTGLSTSNNSEVVREISEKRPKTTFF
                     TRMYLTPPYAKVGFHLVIPLIWEGEEEPFSYIVHTFFAEDYFFPLLATWPGTEETGEI
                     LLLERNNNMIQVINPLKLVPFTAFSLQISIAAENSVEAQAGLGKTGVIMGKDYRGKRV
                     LAYAEQVPNLNWIILSKLDYGEAFASLIPTIVVFIIFIFVAIIALLAGSYVILSTRAL
                     ATFQSKFELLKRTERNEALLSAILERLDSPVVVIDENLEIQLANRSFKERLGNSLPKG
                     LRLPQIDSGIVPSEVQTIEIVDPLGKTLRFYATAIQIVLPDQPALFAYVMRDVTELES
                     MLEQVKTLNLELAQKVEEQTKRISDTSEELRTIASAISFNLIAPLRTIESLSKSLETM
                     AQDKLDSETLDYITNIRKATESMARLTNDLSILLSIDTMQLADEEFDFSIASQEITSE
                     IIKRNPQRRYQITIMPGLKVRGDGNLLKIALRNVLENAIQNCPESLTATIEIGKCGES
                     CIFVQDNGIGISPEEIDSLLRPFSNTKNGQSAGQLNVGFAITKKIIERHGGKLEIVSE
                     FDKGTTVRFDFSSVS"
     assembly_gap    7500..7599
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7600..9663
                     /locus_tag="PLS88_04890"
     CDS             <7600..9663
                     /locus_tag="PLS88_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HOU06714.1"
                     /translation="LSSYEAYGWKRFAISASFSVVILYIAMGSMLGLPLPAFIDHKSS
                     PLAFALVQLALLVPVLIAGKSFYVHGFKSLINLSPNMDSLIAIGTSSAILYSLYSTWR
                     ILGGDHEASMQLYFESAATIITLVMLGKNFESQSKRKTSEAVRKLMKLRPSTATILDG
                     EIERKVSIDEVKPNDLLLVRPGERIPVDGRVKSGLSNVDESMLTGESIPVEKAEGFEV
                     FGGTMNLNGSLVIVAEKVGEDTVLAKIIRLVEEAQSSKAPIARLADQISSIFVPTVMG
                     IALLAALGWLLAGQSVTFALTVFVSVLTIACPCALGLATPTAVMVGTGKAAELGILIK
                     SGEALEIVHKVNTVLFDKTGTITVGKPELTDVVAAEGISEDKVLELAASAELSSGHPL
                     ANAIVEAARDKGLYLSLPDHVETIAGKGIVAESPDIAVGNEALAQSLGILLSAKAPDL
                     LSESTRLAAEGKTVMFVFDNRAPIGLIAVADQPRPESRAVVDTLRKMGIDVALVTGDN
                     RSTADAIAHSVGISEVYSEVLPAEKASIVKGIQASGRKIAMVGDGINDAPALAQADVG
                     IAIAAGTDIAMESADIILMHNNIGDVVSAIHLSREVIKNIKQNLFWAFGYNILGIPIA
                     AGMLYAFGGPLLSPIFAAAAMSLSSVSVVTNALRLKGYKSGMETRSTNSRALEKRRID
                     HEKIA"
     gene            9647..9856
                     /locus_tag="PLS88_04895"
     CDS             9647..9856
                     /locus_tag="PLS88_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOU06715.1"
                     /translation="MKKLLKIEGMTCGHCVMHVQSALEAVPGVKSAKVDLLQRQAIVE
                     GEQLDEQALRAAVTEAGYRVVEIIP"
     gene            complement(9911..11035)
                     /gene="dnaJ"
                     /locus_tag="PLS88_04900"
     CDS             complement(9911..11035)
                     /gene="dnaJ"
                     /locus_tag="PLS88_04900"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOU06716.1"
                     /translation="MAKRDYYEVLGVPKTATKDEIKKAYRKLAIQFHPDKNPGNKTAE
                     EKFKEATEAYEVLGDDQKRQAYDQFGFAGVEGMAGAQQDYSSVFRDFEDIFSGFGDFS
                     SFFGSIFGEGAQRRGETRANHGANLRYDIELPFEKAIFGTTVEISYNKDDICKTCGGS
                     GSRDNLGRKVCSTCKGTGQVRRSSGFFAISQPCPVCHGEGTVIENPCQDCAGSGLVKK
                     KQKIRVTIPPGVEDGKRVTVPGQGNAGPNGGPPGDLHVFIHVRPHEMFERQGNDLYCA
                     VSIDFVTAALGGEVVIDTLEGRKISVQIPAGTQNGKLLRIKGEGVPVSGGKRGDLYIK
                     VFVKVPSRLTKRGREILEELKKTEGETLQPELVKLKDIPR"
     gene            complement(11101..13041)
                     /gene="dnaK"
                     /locus_tag="PLS88_04905"
     CDS             complement(11101..13041)
                     /gene="dnaK"
                     /locus_tag="PLS88_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOU06717.1"
                     /translation="MGRIIGIDLGTTNSCVAVMEGGEPIVIANSEGQRTTPSVVAFTA
                     KGERLVGQPAKNQMITNAENTVFSIKRFMGRRYSEILNEIKLVPFKVIDNGNDVRIEA
                     GGKLYSPQEISAFILQKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRIAGL
                     EVKRIINEPTAASLAYGFNKDQKKEKTIAVYDLGGGTFDISILELGEGVFEVKSTNGD
                     THLGGDDFDRRVMNWLVEEFKNDTGIDLSKDRMAVQRLREASEKAKIELSNMQQTEIN
                     LPFITADANGPKHLQKSLTRARLEQMCMDLIERTREPCIKALADAGLTASQIDEIILV
                     GGMTRMPRVQQMVREIFGKEPSKGVNPDEAVAIGAAIQGGILGGDVKDVLLLDVTPLS
                     LGIETLGGVFTRLIPRNTTIPCRKSQIFSTAADGQTAVSIHVLQGEREMAAQNRTLGR
                     FDLVGIPPAPRGVPQIEVTFDIDANGIVHVSAKDLGTGKEQKIRIEASSGLNEAEIEK
                     MVKEAEAHAEEDRRERERIDARNEADSLIYSTEKSLKEVGDKISASEKTAVDNAIKEL
                     KDVMEGKDTELIKQKTEALKQASYRLSEELYRSASAQGAASEEAGSGSATGESASDSS
                     AGSGDQTRNADDVDYKVVDDDK"
     gene            complement(13116..13847)
                     /gene="grpE"
                     /locus_tag="PLS88_04910"
     CDS             complement(13116..13847)
                     /gene="grpE"
                     /locus_tag="PLS88_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOU06718.1"
                     /translation="MSKHKHEEEQQSEMRNQENSQAQNPKNSPSAPESKIEHEAEPKQ
                     VDQSATDLESQLAAANAEIERLKAQLEELNDKYLRTLAEQVNFRKRVVKEKEEFQQYA
                     VSTLLNDLIPVLDDFDRSLDAVAQNHKDVEKVIEGIRLIQRRLYDTLSKKYGLKQYKA
                     EGTAFDPHLHEAMFSEKGDVHEPKVTQEFLPGYKLHDRVIRTAKVKVMMPDNPAGAPS
                     KTTEARGETQDSASFEGAGENSSLG"
     gene            13961..15400
                     /gene="rpoN"
                     /locus_tag="PLS88_04915"
     CDS             13961..15400
                     /gene="rpoN"
                     /locus_tag="PLS88_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOU06719.1"
                     /translation="MSKFSKARETRSSLTFEESVVHLIVEGEYALQSQRPVLVQQQRL
                     RLNPRMVQAIHLLALPLQELAEQINQELEENPALELLTDSSEISLDFMESRIDNTAEK
                     ENNLNEAEYSDDIYGFNTEDIDNNAIIEQTIATEESLQEHLLGQLGLLPINEQERKIA
                     ERIIQNIDENGFHILPPSELCNDCDPETLNRLLYLLQRLDPPGTCTSDFRESLMVQAE
                     MSSSAPPKTIDVIKDHFEDLRLRNRSPIKKALGIDDRQLDRIVEFIRTLEPFPGRSFS
                     KVRPRYIVPDIAMWLEDGEIKVRLNEDFVPKLGINRLYSELASQQAKGEAERFAREKV
                     ENARFFINSIKKRNETLLKTAYAIAEAQRDFFVAGPKALKPLTLKEIAEKIGVHEATV
                     SRVANGKYVQTDWGTFEMRRFFTNAINQEKGENISKEAAKAEISQIIQELETRGEKIS
                     DRIISEILARRGIKIARRTVAKYRGELGK"
     gene            15466..15762
                     /locus_tag="PLS88_04920"
     CDS             15466..15762
                     /locus_tag="PLS88_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOU06720.1"
                     /translation="MNIDVRSVHFDLSEQSREYLNTKISRIDYAKDMIVDLLFVFTKD
                     ARRFRLEVTANFRWGKQAHIIVTSPELNVGIDVLIDKLDHKIVKEKEKIQKKNK"
     gene            15914..16912
                     /gene="hprK"
                     /locus_tag="PLS88_04925"
     CDS             15914..16912
                     /gene="hprK"
                     /locus_tag="PLS88_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOU06721.1"
                     /translation="MKEKEPVNFTVLDLLSLELKEQNDLKLQCIAGRAGLSREISVPD
                     LNRPGLALAGFYDSFAWERLQIFGRGESAYLHKIEEDGVAAQIYEKLFSYQIPCCIFT
                     NDIRPTDLFIAAAEAVGCPVLTTELNSMELSIRIIRILHDIFAPKMIMHGVLVEVFGI
                     GILLIGESGVGKSETALALIERGHRLVADDVVEIHCLNGSMLMGQGTNKVIGHHMEIR
                     GLGIINVTHLFGVGAIRDKKQIQLVIQIEDWDPKKVYDRIGTDELTMEILGVKVPKLE
                     IPVKPGRNLAIIIETAAMNERLKKMGYHSAREFNQNILRWLESESARTMYFSRDDY"
     gene            17010..17282
                     /locus_tag="PLS88_04930"
     CDS             17010..17282
                     /locus_tag="PLS88_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOU06722.1"
                     /translation="MVELETTIKNRAGIHARPSALIAQTAIKFASRIYLEKEGNRINA
                     KSIMGIITLAASFGTKIKIIADGPDEQDAVKAIAELFESGFNEELG"
     gene            17362..17964
                     /gene="lexA"
                     /locus_tag="PLS88_04935"
     CDS             17362..17964
                     /gene="lexA"
                     /locus_tag="PLS88_04935"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOU06723.1"
                     /translation="MKDLTDRQRQILSFIQEHIQKNNYPPTVRETARAFSISVKGAYD
                     HIKALEKKGYIKTAEKRSRAIELISPPKDNAAVVEIPLLGEIAAGKPIFADENFEKTI
                     SIPAEFVSNHIPHFALRVNGDSMIGAGILSGDIAIIEQSETAKNGDIVVALLDENVTL
                     KRFYIESNRYKLQAENLSYAPIYTQDLRILGRLRGIYRSY"
     gene            17969..19009
                     /gene="holA"
                     /locus_tag="PLS88_04940"
     CDS             17969..19009
                     /gene="holA"
                     /locus_tag="PLS88_04940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOU06724.1"
                     /translation="MADYLISPVWILAGPEVGLKDAFIADIIAKARQLGGDEPEVQRI
                     YAGDSSPSDAIALLQNNSLFSPWVIVEFRNADQISQKTDIDAMVKYCSSPAEQAVLLL
                     ETENYSVPKAIEKVVPPSCRKTFFELFENELAGWIRRELSQSRLSIDEDALESFLELV
                     PHDTNSLKAACLILSASFPPGGMLGASDVEAAILRSKPEDAFTLFEKIAQGDLSLSLE
                     VLNSVLDSRRGDANQIIAALVWSFRRLARIGEAVANGERFEDVCIREQARSKTVQRQL
                     RSGLDHYKPEDCRRIISALSETEAAIRGPLGSAFDRQLLHLLIISIITQKGEGLALAG
                     WSERGLYPQFTN"
     gene            19018..20196
                     /locus_tag="PLS88_04945"
     CDS             19018..20196
                     /locus_tag="PLS88_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOU06725.1"
                     /translation="MSQTVRTIQLGEKTIILVGTAHISKESIEEAKATIIAEQPQRVC
                     VEIDMGRYQSISQESHWEKLDIIKVLKQGNGFLLLANLALAGFQKRLGLDLGTKPGEE
                     MLAAIQTAEEMGIPWSAIDREIQTTLKRAWGKSNLWNKSKLLASLIESAFSREKLSEE
                     DLEKLKESSELEQMMNELANYMPSVKEVLIDERDRYLATKIFLTTEQKVVAVVGAGHM
                     NGIEAWLGKLSRGEIEADVSDIESLPKKGWFAKSAGWLIPVLIVALIAIGFFRSGSQA
                     SLAMIERWVLLNGSLSALGSLLCMAHPVTILASFLLAPLATLNPLLAIGLFAAIIEAF
                     FRKPTVRDAETLADDVTSLKGFYKNRITHILLVFFFSSLGGMIGNFIALPLLASRAIG
                     "
BASE COUNT         5303 a         4802 c         4780 g         5234 t
ORIGIN      
        1 gcgccgaatt caggtaagtg tggtggagta cctatgctct cgtgcaagat aaaatagtgc
       61 aaagcgcgaa tcgtggactc gaaggctata tctgcccatg gaatggcacc agcggtcacg
      121 aactccacgc tgtcgctctc ttctggatcg aggtgaaaat cgtcgatttc cacgatattg
      181 cctcgcgaat agaagtatac atcgcatgtc gcgtagggaa ttccctggta ctggtataca
      241 ttcgggaatg tggcaagaaa attgatatga gtgggagacc aaccgatctc ttcgtagcat
      301 tcacggaggg ctgcgtcttc tgctcgctct ccaggctctg taaatccacc ggggagtgca
      361 aatctgcctt tcattgggtc tttatggcgc cttatcatga cgattttatt ctttgcttcg
      421 attataatgc cagccgctgt agctacattg tggaagtatt caaaaccgca ggtagggcat
      481 ttccacattc gacttcttac actgtaaagt tttgggctgc cacaggatgg gcagaagtgg
      541 aaagcagagg cagcagcacc gatacctcct aagcgctcta gggcatgggc tgtcttctct
      601 cctagtgaac ggatatactc atctgagcag ccattattat ccataatgga caattcagag
      661 atcgccatta tggcagatag ctcgctcgaa ttatgagctg tctcaggttg ctgctttgtc
      721 ttttccatgg aagagcctta ttttaattat atactatgaa tgccaaatac gccaagaaat
      781 gcaaaagccc ggaagctaag agagccccgg atcgagacaa aattaaagga gtcgaagaat
      841 cctcaaaaga aaccatagtg gaaaccgatg ccgaacgccg tgcacgtcta aaggcgcttg
      901 tcaagcaggc accagaatgc ccgggcgtct atcttatgcg agacgatacc tctgaggtta
      961 tctatgtcgg caaagctaaa gtgctcaaga ataggctctc ttcttatttc gctggtaaga
     1021 aggatacgaa gacgcgccat ctcgtatcgc acattaataa tatagaatgg gtgctggctg
     1081 cgcacgaata cgaggcgctc cttctcgaaa acaatttcat aaaagaatat actcctcgat
     1141 acaatatcaa cctaaaagat ggcaaaactt atccttcgat ccgaataaca aatgaagagt
     1201 ttcctcgagc ctttcgcaca agacgcatca tcaatgatgg aagcgagtat tttggccctt
     1261 atccgagcgc ggagaccata gacacatatc tcgatcttat aaagcgaatg tttccgctgc
     1321 ggcgctgcgt caatatgaag aaacatacaa gcccctgtat gtactatcat atcgggcgct
     1381 gtccggctcc ttgtgcggat agaattagcc gcgaggaata tatggagcag gtagacaaag
     1441 tacgcaaatt gctgtcgggc gacactgaat cgctagtcaa agacttagaa cgagagatga
     1501 gtcgagcagc ggagcagctg cgcttcgagg aggctgcacg cctgcgcgaa gccataaggg
     1561 ccgtgcagtc gtttgaaggt cgctcgaatg tggtggactt taatagccta gacgcgcgcg
     1621 attatatcgc ttgggaggca aatggcgatg aggttagcta tgtgatcttc cagatgcgcg
     1681 agggcaagct caagaataga gacagcttta tcgaacactg catagatgag ctcgaggctc
     1741 tcgatagttt cctagcgcac tactatgact caggcagaga gccgccacct caaattttcg
     1801 ttcgaagagc tgacaataca gcaacaattg cgcgctatct ttcggatcgc gcaggcgcgc
     1861 ctgtcgagat aaaaattccg gatagcgagc gacactgggc ggtcatgaat atggcggttc
     1921 agaatgcaaa agaggagctg gcaaagcgtc gtcgcgccca tggagacatt caagcccttg
     1981 tcgaactcaa ggagaggctc ggactcatgg cagttccgat gattatagag ggtttcgata
     2041 tagctcagct tggtggaaag catactgttg cttcgctagt tagttttctt ggaggagtcg
     2101 caaataaaaa gaattatagg tattttaaga ttcgttctgt cgaaggcaag gtagacgatt
     2161 ttgccgctat acgagaggcc gtagcgcgca gatacactag actcatcaat gaggaagccg
     2221 agctgcctga tcttgtgctc gtcgacggag gcgcaggcca ggtctcggca gccaaagaaa
     2281 tcctagacga tcttggcctc gatatcgatg taataggcct tgccaaaaaa aatgaggaaa
     2341 tatggctgcc cgataaaagc gatccgctga ttctgccaaa agactcccca gctttgagaa
     2401 ttcttgtggc tgttcgtgat gagacgcacc gctttgccac tggcctttcg cgcaaactga
     2461 gatcgggcga tgcaaccttc catttgttac aagaggtgcc tggcattggc gatacgcgcg
     2521 ctagaaagat tctcaacact cttggctctc tcgaagcagt agcggaagca gagcctggat
     2581 acatcagcag tattgcgcga gtgaacgagg ataccgcgcg aaaggttatc gagttagcac
     2641 agcgaaccgt gggccttggc tagttttaca aagatttgac tcgatgatag gatgttcgga
     2701 ttggatgaga ttatgagacc ggataaacgg gaaatttgga aaagatgaca ggatgaagaa
     2761 aggatttaca ggataagaat ggtttttggg aaaaagagag gcattctctt ctctctaccc
     2821 aaaataaaat cctgtcaatc ctgcaaaatt ctgataatcc tgtcaaatta tctcgatcaa
     2881 tcccgtccaa aatatttagt gcatgctatc aaaatttaac attattagga ggatttcgat
     2941 gaatacttct gcgctgcagg caattgctct gtctgttcgg agcctgacta tcgacgcgat
     3001 tcagaaggca aattctggtc atcccggcat gccaatgggc gccgcggaac ttggtgctta
     3061 cctttatggt gtcgagctca agtattaccc aaaagatcca aaatggctca acagggatag
     3121 atttatccta tcggctggcc atggctcgat gtttctatat tctcttcttc acatggctgg
     3181 attcgatcta tctctcgatg atatcaaggc ttttcgtcag gttggctcga agtgtccggg
     3241 gcatccagag tatgggcgaa cgccaggagt agaaacgact acgggcccgc tggggcaggg
     3301 aattgctact gcggtcggca tggctatcgc agagcgcaag atgtcggcgc atttcaacaa
     3361 gccaggtcag gagattttcg atcactttgt gtttgttctg gctggcgatg gatgcatgat
     3421 ggagggggtt gctagcgaag cctgctcgct ggctggccac ttgggccttg gaaatcttat
     3481 cgtctactat gattcaaacc atatcagtat cgatggtcct acggatatca ctttctcgga
     3541 ggatgtcgca aagcgtttcg aggcctatgg ctggcaggtg cttcgaggct ccatgtatag
     3601 tttcgaggat atcgcctctc tcaccgccag agccaaggcg gaaaaagata ggccttctct
     3661 catcgtcttg gacagcgtta tcggtaaggg agctccgaca aagcaaggta cgagtagtgc
     3721 ccacggttcc ccgctgggcg aggaagaagc cctcaaggcc aaggaagccc ttggtattcc
     3781 tatcgacaag cccttctggg tcgctccaga ggcctattct tacttcgaag agcatcgaaa
     3841 agagctggaa aaagactatg ctgcttggca gcttcgcttc gaagcatgga aaaaggccaa
     3901 tccttccctg gcccaagaac tagcaatttg gctcagcggc aaggaaatga acaaactcga
     3961 tttaccttct tttgtcgtcg gcgaaaaggt tgccacacgg aatgcttctg gtaagtgtct
     4021 tgcggcaatt tcggaggctt ggccaaactt cattggcgga tcggctgatt tgactagccc
     4081 caatgtaagt cagctaccgc aaaatgcaga tggcactcct ctcgtctttt cgaagtccaa
     4141 tccgaatggg cgatatatct actttggagt gcgcgagcat gctatggcgg caatagccaa
     4201 tggaattgcg ctatatggtg gcttgcgccc ctttatagca acattcctcg tattctcgga
     4261 ttatctgcgt ccagctctgc ggctttccgc attgatggga ttgcccgtaa tctatgtgct
     4321 cacccacgat tcgatctatg tgggcgaaga tggaccaacg catcaaccga tcgaagctct
     4381 cgcggcacta agggcaattc cgaaccttag agtcctacgg cccgtagatg ccgaagaaac
     4441 agccgtagca tggagagtgg caatggagcg cttggatggg cccaccgtgc ttgtgctttc
     4501 gcgacagaat ctgcctgtcc tggagaaata tgaaagcgat tggccttcca agatcgatct
     4561 cggagcctat gtggttaaag acagcgatcg cgctcccgag gtgacgcttg tcggttctgg
     4621 gtcggaagtc tcccttgcaa tctcagcagc cgagattgtg aaaagtaagc gacaagaaac
     4681 cgcaattcga attgtttccg tccccgatag aagagctttc tatgcagcct gtcctctagt
     4741 tcgagaagcc atcctttctc ccggatctct ggtctttgta gccgaagctg gggtagcaca
     4801 aggctgggag cgcattgctc ccgcagatca gattctttcc atagagcgat tcggcgaatc
     4861 gggcccaggg gacaaagtcg cggaacatct tggttttaca gcacaagttc ttgctcagat
     4921 gatcctagcc aaattgaaat aaaatcccct ttacttgtgc ggccttgatt tgtatcatta
     4981 ggtgtaagtg cttatttagg ccaagcttgt gtaccgagct tggccagtta gcgctcgaat
     5041 gctcattcga tatagaattg aataaaaatc atcattgtta aacaaaatag ttattataac
     5101 actgtaaagt ttcaattccc ttggaaataa aagcactctg tattagtatt agatatcact
     5161 tctattgcga gggatatgag caagattgtg cgagaaggag tttagggcat ggttcgatct
     5221 aatgccaaaa gacatagtat tcttgctcac cttagagaat tgcgtcctat cgagcgtttc
     5281 tggtttgtca ttttcttttt gattctgctc ggttcgcttg tgataggcgc ttattaccaa
     5341 tggcagaata tcctgcacga caagaaactc tacatcgaac tcatcggcaa atcgaaaata
     5401 agccagatcg aattatggta cacggcgcat attacaaagg cggaagaatt acctaattca
     5461 ctggcttttt taaatcttgt ctctgaagcg attacggatc cttcgaaaga gaatctcgag
     5521 cgcctcgatg aatacttaag gccatcgctt agctcttaca attatgccga cactgcagtg
     5581 ctaacttcaa acctcgatgt tctctataca cttactggct tatcgacttc caataattcc
     5641 gaagttgttc gcgagatatc cgaaaaaaga ccaaaaacta cattctttac cagaatgtac
     5701 cttactcctc cctatgcaaa agtgggtttc catcttgtga ttcctctcat ctgggaaggc
     5761 gaagaagagc cttttagtta tattgttcat acgttttttg ccgaagatta tttctttcct
     5821 ctcctcgcta catggccagg cactgaagaa acaggcgaaa ttctgctcct cgagcgcaac
     5881 aataatatga tacaggttat caatcctcta aaactggtcc catttacggc cttttccctt
     5941 cagatttcca ttgcggcaga gaattctgtg gaggcacaag ctggtcttgg aaagactggt
     6001 gttataatgg gcaaggatta ccgtggtaag cgtgtcctag cctatgccga acaagtccca
     6061 aatttaaact ggataattct ttcgaagctt gactatggag aagcttttgc ttcattgatc
     6121 ccaacgattg tcgtttttat tattttcatc ttcgttgcta taatagcgct attggcggga
     6181 tcatatgtga ttctttccac gcgcgcgctt gccacttttc agtctaaatt cgaactgctc
     6241 aagcgtaccg aacgcaatga agcactactg agcgccatac ttgagcgtct cgattcgcca
     6301 gttgtagtaa tagatgagaa tctcgagatt caactggcaa atcgctcttt caaggagaga
     6361 ctgggaaatt ctctcccaaa gggtttgcgg ttaccacaga tagattctgg tattgttccg
     6421 tccgaggttc agacgatcga gattgtagat ccgctaggaa agactctgag gttttatgca
     6481 acggcaatcc agatagtact gccagaccaa ccagcacttt tcgcctatgt aatgcgcgat
     6541 gtgaccgaac tcgaatcgat gctcgaacag gtcaagactc tcaaccttga gctcgctcaa
     6601 aaagtggaag agcagacaaa gcgcatttct gataccagtg aggaattgcg tacaattgct
     6661 tcggcaattt cttttaactt gattgcgcca cttcgcacca ttgagtcctt gagcaaatcg
     6721 ctggaaacca tggcacagga taagctagat agcgaaactc tagactatat aacaaatatt
     6781 cgaaaagcta ccgaaagcat ggctagactc acgaacgacc tttcgattct tctttcgata
     6841 gacacaatgc agcttgccga cgaagaattc gatttcagca ttgcttctca agagattact
     6901 tcggagatca tcaaacgcaa tcctcagagg cgctatcaga taaccattat gcctggacta
     6961 aaggtacgcg gagatggcaa tctattgaaa attgcgctac gcaatgttct tgaaaatgct
     7021 atccagaatt gcccagagag cttgaccgct acgatagaaa taggcaaatg cggagaatcc
     7081 tgcatctttg ttcaggataa cggcattgga atatcgcccg aagagatcga ttctctatta
     7141 aggccattct ctaacaccaa gaatgggcaa tctgcaggcc agctgaatgt tggctttgcc
     7201 ataacaaaaa aaataattga gcgccatggt ggaaaactcg aaatagtaag tgaatttgac
     7261 aaagggacta cggttagatt cgatttctca tcggtatctt gaaactttga tatacatgag
     7321 accgaaaact tgctcacaga cagcctaaaa tgaatgttta gatttcttac agtgcttttt
     7381 tagtcattag acaacgcatt agagatttta tcttgtttat ttggggattt gagagttttg
     7441 ctctagatag cgaattagga atcttgctaa aattactata tatttatttt gatagattan
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tttcttccta tgaggcctat
     7621 gggtggaagc gttttgctat ttctgcgtca ttttccgtag ttattctata tatagctatg
     7681 gggtctatgc ttggtcttcc tttgccagct tttatcgacc ataagagctc tccattggcc
     7741 tttgctctgg tgcagcttgc cttgctcgtt ccagttctca tagccggcaa gtccttctac
     7801 gtccatggat tcaagtctct cataaatctt tcacctaata tggacagcct aattgcgata
     7861 ggcacaagtt cggcgattct atatagccta tattcgacct ggcgtattct gggtggagat
     7921 cacgaagcca gtatgcagct ctatttcgag tccgctgcga ccatcatcac tctagtcatg
     7981 ctgggcaaaa atttcgaatc tcaatccaag cgaaaaacct ccgaggccgt ccgcaaactc
     8041 atgaagctgc gcccgagtac cgcaactatt ctagacggag agatcgagcg caaagtctcc
     8101 atcgatgagg tgaagccaaa cgatcttctc ctggttcgcc ctggagagcg catacccgta
     8161 gatggaagag taaaaagcgg actttccaat gtagatgaat ccatgctcac cggggaaagc
     8221 attcccgtcg aaaaagccga aggcttcgag gtatttggcg gaaccatgaa tctcaatgga
     8281 tctcttgtca tcgttgcgga aaaagtaggc gaagatacag tactagctaa aatcatacgt
     8341 cttgtggaag aggctcagag cagcaaagcg cccatcgcaa gactcgcgga tcagatatcg
     8401 tcaatattcg tgccaactgt gatggggatt gcactgcttg ccgcccttgg atggctcctt
     8461 gccggacaga gcgtgacatt tgcattgacg gtctttgtat cggtcctcac tatagcttgc
     8521 ccttgtgcgc ttgggttagc tacgcccacg gcagtcatgg taggaactgg taaggcagca
     8581 gagttgggca tactcatcaa atcgggcgaa gctctcgaga ttgttcacaa agtgaacact
     8641 gtgctgttcg ataagacggg cacaataact gtcggtaagc cagagctcac cgatgtcgtg
     8701 gcggcagagg ggatatccga agacaaggtg ttagagcttg ccgcttccgc tgaactctcc
     8761 tctggacatc ctctggcaaa tgccattgtg gaggctgctc gcgataaagg tctttatctt
     8821 tctttgccgg atcatgtcga gactatagct ggcaaaggaa tcgtcgcgga atctccagac
     8881 attgcggttg gcaatgaagc tcttgcccaa tcgcttggca ttttgctatc tgcgaaggcg
     8941 cctgatctat tgagcgaatc gacccgtctt gcggcagagg gcaaaacagt tatgttcgtc
     9001 tttgataata gggcgcccat tggccttatt gcggttgcag atcagcctag accggaatcg
     9061 cgggctgttg tggataccct gagaaaaatg ggtatcgatg tcgcgttggt aaccggcgac
     9121 aaccgtagca cggcggatgc catcgcccat agcgtcggaa tctccgaagt ctattcggag
     9181 gtccttcctg cagagaaggc ctctattgtc aaaggcattc aggcttctgg cagaaaaatc
     9241 gcaatggtag gcgacggcat aaacgatgcg ccggctcttg cgcaggccga tgttggcatt
     9301 gcaatagcgg ctggtacgga tatcgcgatg gagagcgcag atatcatcct aatgcacaac
     9361 aacatcggtg atgttgtcag tgcgattcat ctttcgagag aagtaatcaa gaacataaaa
     9421 cagaatctat tctgggcatt cgggtataat atactcggta tacctatagc ggctggcatg
     9481 ctatatgctt ttggtgggcc tttgctttcg ccaatttttg cggctgctgc catgtccttg
     9541 agctctgttt ctgttgtgac caatgcgctt cggctcaagg gttataagag cggcatggag
     9601 accagatcga caaactctag agctctagag aaaaggagga tagatcatga aaaaattgct
     9661 taaaatcgaa ggaatgacct gcggtcactg tgtaatgcat gtgcaatccg cgctcgaagc
     9721 agtgcctgga gtcaagagcg ccaaggtcga ccttctccaa cgccaggcta tcgtggaggg
     9781 cgaacagcta gacgaacagg cattgcgcgc tgccgtgacc gaagcaggct atcgcgtggt
     9841 ggagattatc ccataagaaa aaggctgcct gatttctccg gcagccttct tcgattatct
     9901 tggtatatta tcaccttggt atatctttta acttgacaag ctcgggctgt aaagtctcgc
     9961 cctctgtttt cttgagctct tccaagatct cgcgaccccg tttggtaagc cgagaaggaa
    10021 ccttgacaaa aaccttgatg taaaggtctc cgcgtttgcc gcccgaaaca ggaacaccct
    10081 cgccttttat acggagcagc ttcccgtttt gggtgccagc cggtatctga acggatatct
    10141 tccgcccttc caatgtgtct atcacaactt cgccgcctag cgcggcagtt acgaaatcta
    10201 tcgatactgc gcaatataga tcgttcccct gacgttcgaa catttcgtga ggccgcacat
    10261 ggatgaagac atgcagatct cccggaggcc ccccattggg accggcgttc ccctgaccgg
    10321 gaaccgtgac tcttttgcca tcctcgacgc cgggaggtat ggtgactctg attttctgct
    10381 ttttcttgac taacccgctt ccagcgcaat cctgacaggg gttctctatg acggtcccct
    10441 ctccatggca taccggacat ggttgggaaa tagcaaagaa tccagaactt ctgcgtacct
    10501 gcccagttcc cttgcaggtc gaacagactt ttctgcccag attgtcgcgc gaaccgctcc
    10561 ccccgcaggt tttgcagata tcatctttgt tgtatgaaat ttctaccgta gtgccgaaaa
    10621 tcgccttttc aaatggaagc tctatatcgt atcttaagtt agcaccgtga tttgcacgtg
    10681 tttctccacg acgctgcgct ccttcgccga aaatcgaacc aaaaaacgaa gaaaaatcac
    10741 caaacccgct gaaaatgtcc tcgaagtcgc gaaatacgct ggaatagtcc tgttgagctc
    10801 ccgccattcc ctcgacacca gcaaagccga actgatcgta agcctgacgt ttctggtcat
    10861 ctcccagaac ttcgtaggct tctgtcgctt ccttgaattt ttcctcggct gtcttgttcc
    10921 ccgggttctt gtccggatgg aactgaatgg caagttttct ataggccttt tttatttcgt
    10981 cttttgttgc ggtttttggg acaccgagca cttcgtaata atcacgcttg gccacttcga
    11041 aaatcctttg atgaaaaggt ggctgaactg taatccagcc acctcgatat gctctctatg
    11101 ttatttgtcg tcgtcgacca ctttgtaatc gacatcgtcg gcattccgcg tctggtcgcc
    11161 agagccggca gaactatcgg aagcactctc tcctgtagcg ctgccactgc cagcttcttc
    11221 gcttgcggca ccttgggccg aagcgctgcg gtacaattct tctgacagtc tataggatgc
    11281 ctgttttagg gcttcggttt tctgcttgat gagctcagta tccttgcctt ccattacatc
    11341 cttgagctcc tttatggcat tatctaccgc agtcttttcg ctcgcgctta ttttatctcc
    11401 aacttccttt aaggatttct cagtgctata gatgagcgaa tcagcctcat tgcgcgcatc
    11461 gatgcgttcc cgctcacgcc tatcttcttc ggcatgagct tcggcttctt tgaccatctt
    11521 ttcgatttca gcctcattga gcccgctcga tgcctcgatt ctgatctttt gctctttgcc
    11581 ggttccgaga tcttttgccg aaacatgtac aattccgttt gcgtcgatat cgaaggtcac
    11641 ctcgatctgt ggcacgccgc gaggcgctgg aggaattcca acaaggtcaa atctcccgag
    11701 cgtgcggttt tgcgctgcca tctcgcgctc tccctgcagt acatgaatgg atacggctgt
    11761 ctgcccatcc gctgctgtag agaatatctg gctcttgcgg catgggatcg tcgtatttcg
    11821 cggaatgaga cgcgtaaaca ctccgcccag tgtctcgata ccaagagaaa gcggcgtaac
    11881 gtcgaggagg agtacatcct tgacgtctcc acccaagatg cctccctgga tcgccgcgcc
    11941 gatggcaact gcttcgtcgg gattgacccc cttggaaggc tccttaccga atatctcgcg
    12001 aaccatctgc tgcacgcgag gcatacgggt cataccacct acgagaatta tctcgtctat
    12061 ctgtgaagcc gtaagccctg catctgcaag tgccttaatg caaggctcgc gggttcgctc
    12121 tataaggtcc atgcacatct gttcgagcct ggccctggtg aggcttttct gtaaatgctt
    12181 tggcccgttc gcgtccgcgg ttataaaggg cagattaatc tcggtttgct gcatattgga
    12241 gagctcgatc ttggcttttt ccgatgcctc gcgcaagcgc tggacagcca tacgatcttt
    12301 ggataaatcg atgcccgtat cgttcttgaa ttcttctact aaccagttca taactcggcg
    12361 gtcgaaatcg tcaccgccaa ggtgagtgtc gccattagtc gatttgactt cgaatacgcc
    12421 ttctccaagc tccagaatcg aaatatcgaa ggtgccgcca cctaggtcgt acaccgcaat
    12481 ggtcttttct tttttctgat ccttgttgaa gccgtaagca aggcttgccg ccgtgggttc
    12541 atttattatg cgctttactt cgagaccagc tattcgacct gcatctttgg tggcctgtcg
    12601 ctgtgaatcg ttgaagtagg caggaaccgt gattacagct tccgtaaccg gctcacccag
    12661 gtaatcttcc gcggtctttt tcattttctg aagaatgaag gcgctgatct cctgagggct
    12721 gtagagcttg ccaccagcct cgatgcgcac atcattgcca ttatctatta ccttgaatgg
    12781 aacaagcttg atctcattca agatctcact atagcggcga cccatgaatc tctttatcga
    12841 aaatacagta ttctctgcat ttgtgatcat ctggttcttg gcgggttggc ctacgagcct
    12901 ctcgcctttt gcggtaaagg caacaacaga aggcgttgtc ctttgtcctt cagaattggc
    12961 aattacaata ggctctcctc cctccatcac tgcgacacag gaattggtgg tgcccaaatc
    13021 aatgccgata attctaccca tatttctttc tccttctttc ttgtctatct aatctaaaag
    13081 ttacaaaact atctactaca aagcaaatca aactcttatc ctaaagagct attctcccct
    13141 gcaccttcaa atgatgcaga atcttgagtc tctcctcgtg cctctgtggt tttagatgga
    13201 gctcccgcag ggttgtctgg catcataacc ttaaccttag ctgtacgaat aactcgatca
    13261 tgcaacttat atccgggcaa gaactcttgg gtcacttttg gttcatgcac atcgcctttc
    13321 tcggagaaca tcgcttcgtg caaatgcgga tcaaaagcag tcccttcggc cttatattgc
    13381 ttgaggccat atttcttgga gagagtatca taaagccttc tctgtatgag gcgaatgcct
    13441 tcgatgactt tttctacatc tttatgattc tgcgcaactg catccagaga tcgatcgaaa
    13501 tcatcgagca ccggtataag atcattcaaa agcgtagaga cagcatattg ttggaattct
    13561 tctttttctt ttacaacccg ttttctaaaa ttaacttgct cggcgagcgt gcgcagatac
    13621 ttgtcgttca actcttcgag ttgagccttg agtcgctcta tctctgcatt agctgccgca
    13681 agctgagatt ctagatctgt ggcagactga tccacctgtt taggctccgc ttcatgttct
    13741 attttggact ctggagccga aggcgaattc ttcgggtttt gagcttgaga gttctcctgg
    13801 tttctcattt ccgattgttg ttcttcttca tgcttatgct tagacattct tcaccctatt
    13861 aatagaaaat tttattgtgg tctttcctaa agaaaatact aattaaaaat catgatcgtc
    13921 aatacaaatg ctgcaatcat tcgtaagatt ttttcattgg atgtcaaaat tcagcaaggc
    13981 gcgtgaaacc cgctcatcct tgacattcga ggaaagtgtc gtacatttaa tagtagaagg
    14041 agagtacgca ttgcaatccc aaagaccggt tcttgtacaa cagcaacgct taagacttaa
    14101 cccgagaatg gtacaagcaa tacatctgct cgccctccct ctgcaagaac tcgccgaaca
    14161 gataaatcag gagctcgagg aaaatccagc gctcgaacta ctaaccgact caagcgagat
    14221 ctcgctcgat ttcatggaat cgcgcataga taatactgca gaaaaagaga acaacctcaa
    14281 tgaagccgag tactccgacg atatatacgg attcaatacc gaagatatcg ataataatgc
    14341 cataatagaa caaacgatag caaccgaaga gtcgctccaa gaacacctac ttggccagct
    14401 tggactcttg cctatcaatg aacaagagcg aaaaatcgca gaacggatca tccaaaatat
    14461 cgatgaaaat ggttttcata ttctgccgcc ttcggaacta tgcaatgatt gcgaccctga
    14521 gactctaaat cgcttgctat atctcctgca aagactcgat ccgccaggta cctgcacttc
    14581 agacttccgt gaaagcctca tggtacaagc agagatgtcc tcctctgctc ctccaaagac
    14641 gatcgatgtc attaaggatc acttcgagga cttaagactt cgcaaccgaa gcccgattaa
    14701 aaaagcgcta ggtatagatg atcgtcagct cgatcgtata gttgaattca ttcgaacctt
    14761 ggaaccattc cccggtcgct cattctccaa agtccgtccc cgttatattg tgccagatat
    14821 cgcgatgtgg ctggaggatg gcgaaatcaa agtgcgatta aatgaggatt tcgttcccaa
    14881 gctcggaatc aatcgcctat atagcgagct tgcttctcag caagcaaaag gcgaggctga
    14941 gagatttgcc cgcgaaaagg ttgaaaatgc gcgctttttt atcaatagca taaaaaagcg
    15001 caatgaaact ttgttaaaaa ccgcctatgc cattgccgaa gcccagagag atttcttcgt
    15061 ggctgggcca aaggcactca aaccccttac attaaaagaa attgcggaaa agatcggagt
    15121 tcacgaagcg acagtttctc gcgtcgccaa tggcaaatat gtgcagaccg actggggcac
    15181 tttcgaaatg cgccgttttt ttaccaatgc tataaatcaa gaaaagggcg aaaacatctc
    15241 caaggaggca gcaaaggccg agatttctca gataattcag gaactcgaaa cgaggggtga
    15301 aaaaatctcc gatcgtatca tttctgaaat cctcgcccgg cgaggaataa aaatcgcacg
    15361 ccggacggtg gccaaatatc gtggcgagct cggaaaatag accgagctaa tgattcctat
    15421 gcgcatggcc tagccatagc gtaagcaatt cttgaggagg tcattatgaa tatcgacgtt
    15481 cgttctgtgc acttcgatct tagtgagcaa agtagagagt atttgaacac gaaaataagc
    15541 cggatcgatt acgcaaagga catgatcgtc gatcttctct tcgtctttac taaagacgct
    15601 aggcggttcc gcttggaggt aaccgcaaac tttcgatggg gcaaacaggc gcacatcatt
    15661 gttacctctc cagaactaaa tgtgggcatc gatgtgctta tcgacaagct agatcataaa
    15721 atcgtaaagg aaaaagaaaa gattcagaaa aagaataagt gagagaagct tgcacggcgg
    15781 cggttcttca tgcagacgga ggccaaaaaa cagctcaaga atgaggcctt tcgagactgc
    15841 cattgcagcc ggctgagaac cgccgttttt atattagatt taccactctt gcaggatgat
    15901 gtagaataat attgtgaaag aaaaagaacc tgtcaatttt actgtcctcg atcttcttag
    15961 cctcgaacta aaggagcaaa acgatctcaa gctccaatgc atagcgggtc gagccggact
    16021 tagtagagag atcagtgtac ccgacctgaa tagaccgggg ttagctttag cgggattcta
    16081 cgattcattt gcttgggagc ggttgcaaat attcgggcga ggcgagagcg cttatctcca
    16141 taaaatcgaa gaagacggag ttgccgctca gatatacgaa aagcttttct cataccaaat
    16201 cccttgttgc atttttacca acgacattag accgaccgat ctctttatcg cagccgccga
    16261 agccgtaggc tgccctgtac ttacgactga actgaattcg atggaattat ccattcggat
    16321 cattcgaatt cttcacgata tcttcgcgcc aaaaatgatt atgcatggcg tgctagtcga
    16381 agttttcggc ataggaatat tgttgatagg cgaatcaggc gtaggaaaga gcgagacagc
    16441 tttggcattg atcgaaagag gccataggct tgtggccgac gatgttgtgg agatacattg
    16501 ccttaatggc agcatgctca tgggccaggg gacaaacaag gtaataggcc atcacatgga
    16561 gatacgaggt ctcggaatca tcaatgtgac acatctattc ggagtaggcg ccattcgaga
    16621 caaaaaacag atacaactcg taatacaaat cgaagattgg gatccaaaga aggtctacga
    16681 ccgaatcggc acagacgaat taaccatgga aatactcggc gtaaaggttc cgaagcttga
    16741 aatccctgta aaacctggcc gcaatttagc tatcataata gaaacggctg cgatgaacga
    16801 gcgactaaaa aaaatgggat atcacagcgc aagagaattc aatcagaata tcctgcggtg
    16861 gctcgaaagt gagagcgctc gcaccatgta tttttcccga gacgattatt agattttttt
    16921 actcatgtat tgttgacctt attgaatgca agcctgcatt cgaagtatca tgactgacat
    16981 tggccttgaa tcttctactt ggagccctta tggtagagct agagacaaca attaagaatc
    17041 gtgccggtat ccatgcccgt ccttctgcgc ttatagccca aactgcaatc aaatttgcat
    17101 ctcgtatata tctcgaaaaa gaaggaaaca ggatcaatgc taaatccata atgggaataa
    17161 ttactctagc cgcgagcttc ggcaccaaaa tcaagataat agccgatggc cccgatgagc
    17221 aggatgcggt aaaagccata gcagaactct tcgaatccgg cttcaacgaa gagctcgggt
    17281 aaaaaagggc ctatagaaat aaagggcttg cgtgttttgg cattctgtgc tgtatactat
    17341 acaaaggcac aggagtctgc attgaaagat ctcactgatc gacagagaca aatccttagc
    17401 ttcatccagg agcacattca gaaaaataac tatccaccaa cggtaagaga aacagcgcgt
    17461 gccttctcaa tctcggtcaa gggtgcttac gatcacataa aggctctcga gaaaaaaggt
    17521 tacatcaaaa ccgctgagaa acgctctcga gcaatcgagt tgatctctcc acctaaagac
    17581 aatgcagctg tagtggagat tccgcttttg ggcgaaatcg cggccggcaa gcccatcttc
    17641 gcagacgaaa atttcgagaa aaccatctct attccagccg aattcgtaag taatcatatt
    17701 cctcactttg cgcttagggt aaatggcgac agcatgattg gtgctggaat tctttcggga
    17761 gatatcgcca taatcgaaca aagcgagacg gcaaagaatg gcgatatcgt tgtagccctt
    17821 ctagatgaaa acgtaacgct caagcgattt tatatcgaaa gcaaccgcta caagctacaa
    17881 gcagaaaatt taagctatgc tccaatctat acccaggatc tgaggatact tggcagactt
    17941 cgcggtatat ataggagcta ttgatcccat ggcagactat ctcatttctc ctgtctggat
    18001 actcgcaggc cccgaagttg gattaaaaga cgcttttata gctgacataa tcgctaaggc
    18061 aagacaactc ggaggcgatg aaccagaggt tcagagaatt tatgctggcg atagttcgcc
    18121 ctcagatgcc atcgcgcttc tacaaaataa ttcgctcttt tccccctggg ttattgtgga
    18181 attccgaaat gcagatcaaa ttagccagaa gacggatata gatgcaatgg tcaaatattg
    18241 cagctctcct gcagaacaag ccgttttgct gctcgaaaca gaaaactatt cggttcccaa
    18301 ggcaatagaa aaagtcgtcc caccatcgtg ccgcaagacc ttcttcgagc tcttcgaaaa
    18361 tgaattagcc ggatggatac ggcgagaatt gagtcaatct cggctctcta tagacgaaga
    18421 cgcgcttgaa tccttccttg agcttgttcc gcacgatacc aactctctta aagcggcttg
    18481 ccttatcttg agtgcaagtt ttccgcctgg aggcatgctt ggagcttcgg atgtggaggc
    18541 ggcgattctg cgctcaaagc cagaagatgc ctttactctt ttcgaaaaga tagcacaggg
    18601 agatctgtcg ctttctctcg aagtgctcaa tagcgttttg gactcacggc gtggcgatgc
    18661 caatcagata atagccgctc ttgtttggag ttttcgcaga cttgcgcgca taggggaagc
    18721 agttgccaat ggtgaacgct tcgaagatgt atgcatccga gaacaggctc gctccaaaac
    18781 tgttcagaga caacttcgta gtggccttga ccattataag cctgaagact gcaggcggat
    18841 catctctgca ctctcagaaa ccgaggccgc tatccgaggc ccacttggat ctgccttcga
    18901 ccgccaattg ctgcatctgc tcataatctc tataatcaca caaaaaggtg aagggcttgc
    18961 gcttgccgga tggagcgagc gaggcttata tccccaattt acaaattagg acatcttatg
    19021 tcgcagactg tcagaactat tcaactaggt gaaaaaacta tcatactcgt aggtacggcc
    19081 catatatcga aggagagcat cgaggaagcc aaagcgacga tcattgcgga acagccccag
    19141 cgcgtatgcg tagagatcga tatgggtcgc tatcagtcga tcagccagga aagccattgg
    19201 gaaaaactcg acatcataaa agtgctaaaa cagggaaacg gctttctatt gcttgcaaat
    19261 ctggcactcg cgggctttca gaaacgctta ggcctggatc tcggaactaa acctggcgaa
    19321 gagatgcttg cagcaatcca aaccgccgaa gaaatgggaa ttccctggtc tgctatagat
    19381 cgagaaattc aaacaacgct caagcgagca tggggaaaat ccaatctctg gaacaaatcg
    19441 aaacttctag caagcctaat agaatcggcc ttttcacgcg aaaagttgag cgaagaagat
    19501 cttgaaaagc tcaaagagag cagcgaactc gagcaaatga tgaatgagct cgccaactat
    19561 atgccatcgg tcaaggaagt actaatcgat gagcgagatc gctatcttgc cactaagatt
    19621 ttcctgacca cagagcaaaa agtagtcgct gtagtgggcg ctggccacat gaacggtata
    19681 gaagcttggc tcggaaagct gtcccgaggc gaaatcgaag ccgatgtctc cgatatcgag
    19741 agccttccta agaaaggctg gtttgccaaa tcggccggat ggctaattcc ggttctcata
    19801 gttgctctca tagccatagg tttttttcgt tccggtagcc aggcgagtct ggccatgata
    19861 gaacgctggg tgctcctaaa tggttctttg tctgcgctag gctcactctt gtgcatggcg
    19921 catccagtga caattctcgc ctcgttttta ttagcaccgc ttgcgacact gaatccattg
    19981 cttgctatag gactgtttgc agctataata gaagcctttt ttcgtaagcc caccgtgcgc
    20041 gacgcagaga cgctggccga tgatgtgacg agcttgaaag gcttctacaa gaatcgaatc
    20101 acccatattc tgctggtctt tttcttttcg agcctaggcg gaatgattgg aaattttatc
    20161 gcactcccgc tcctcgcttc tcgcgcaatc gggtgaatac tgttacgaac cttgtgtat
//
LOCUS       DAOSOB010000033        19474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_2804_length_19474_cov_78.336166, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000033 DAOSOB010000000
VERSION     DAOSOB010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 19474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19474
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2804_length_19474_cov_78.336166"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..606
                     /locus_tag="PLS88_04950"
     CDS             124..606
                     /locus_tag="PLS88_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06726.1"
                     /translation="MKRKTKAILFLALGIAILTAGCTIVVTPAGEQCSLYYTWEGSQE
                     KYAVWTLYSNGTFKDNYQGRGIWSADGSYFYLAYNNSSYFYQGTVYTGTIYSPDYMNG
                     VMSGPDKYGIEQYGIWEAYRGVHGYRALTPSSEKDPGSKTLSPSGEPIENHENEETNE
                     "
     gene            603..1454
                     /locus_tag="PLS88_04955"
     CDS             603..1454
                     /locus_tag="PLS88_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOU06727.1"
                     /translation="MKLHRKIALASLILLAFASYTFAQVKVSLTIQANVQNAQVYIDD
                     NLAGYASPNLSLLIFPGTYTIRVVKNGYQEYRTTITAAATPITLFATLSGGAPQTPST
                     PPAPSTPSSPSTPPSPPPYTLPLPITIPEPGFQLSIDANIDGARVYIDNVYVGITPYR
                     VTQYRGGHVIRISAPGYADYVESLYLYRSTVLNAYLSPLPVSYEIRLPDNLQLEWRTN
                     NKYHDKFQAVQLYLDNKRLDSLRGELLPGLHTIAIDYYNLHLEDSFDIKPGQPVILQL
                     GLGVKVY"
     gene            complement(1592..1665)
                     /locus_tag="PLS88_04960"
     tRNA            complement(1592..1665)
                     /locus_tag="PLS88_04960"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1629..1631),aa:Pro,seq:ggg)
     gene            complement(1743..2825)
                     /locus_tag="PLS88_04965"
     CDS             complement(1743..2825)
                     /locus_tag="PLS88_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOU06728.1"
                     /translation="MKSSSSERPKKVSWQLNNERLKSKPKYNLEKVSWQLNSSKNSDQ
                     QLPLYNPAISCFLEEIYAECNRSERLKLDPLAIVKRYKAREDMEVAGLICSTLAFGSV
                     ELIMRACEKALEPLGEHPAKTLREMASKEIELAWSSFKYRFCFPKDMIALMWAIHDAL
                     NCYGSLEALFLECDRKWLQEKPEEACDFAANQQEVSKYEICTGRHESLSCKMSQQQNI
                     LAASSMFVRRMHELALEAVPEGLRKNLLPDPANGSAAKRLFLFLRWMVRRDEIDPGCW
                     NSVSPSRLIVPMDTHMIRTCTERLGFLPQRKIQRKSGVAVNLRDALRVTDAFRLYAPE
                     DPVKYDFALTRPGIDPHPGDERFLCL"
     gene            complement(2864..4858)
                     /locus_tag="PLS88_04970"
     CDS             complement(2864..4858)
                     /locus_tag="PLS88_04970"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU06729.1"
                     /translation="MKIKYIFLLTIAALSVLSFSAPTWSYAQQNLPKTDVEQLKKIST
                     EAKDIETLIVLATPHSLAQARSKTKASPYLSEPDKAALDAIAKSIGLIVYGPKNAVFE
                     IPAAAKGADQKYITCLAGLSDASAGKTPKVFENGSCSLMSELISALPFFRDSRNEIRA
                     QALASISRLEDLGGISAVPSLVRGQIAFEAKQYAEAADEFQKALELDTLSKKSACGLA
                     RAYLAQGKPEKAKQILDPIISIALVESAKETSTKDGYNKKPSSNKGVSAGGGYIETTE
                     IEQDTFSADLKSLYGIALYNLNNILDAEPWLIAALNEDPSRTDLLVPIAHAAMQKRDY
                     ASAWKNLESASKIASKDRTWLILKSQYALENSRQTDAERFARTAVSYYPQDPVALAQL
                     VRVLQESTDAQRHLEAADLAKKVLDITKNEVLNLTPLEQALRKQAEDKAFQFLVMENY
                     NNQDWIMASKYLQEAGGVPLDKEMVAVILRKSGNVQEALQFALSWYLQEPTSEKAVEA
                     YLRSLAMATGGGLASAAPTRDVPTAIVPGLPGLGMSQDEGANSALLDIVLKFIAAPFS
                     KELKSFLYYMSATLQSDENKAISQLKDALAERADNVEALVSLASIYINRYNRQSDKSD
                     TTNRDKAVLYLEQAKSLHPNDDEILARIKQLEAELGYSSR"
     gene            5074..6174
                     /locus_tag="PLS88_04975"
     CDS             5074..6174
                     /locus_tag="PLS88_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU06730.1"
                     /translation="MKSARIGAFTAPLLISILIVFFMGLWGLRVSGITFSNLGEFSIS
                     NKSLRRELRDALHVFNSPQLKNSGYYSIQRMLAAKRISTILIKDGQKASAARFLSSLA
                     DEHDDYEAWYLFAAGAIYESMDSSPIARIFYERILKNLPDMTIDGQSIHRISLIKLLE
                     GDSPPEKKAHWYGEFIRRFPEAEEVGPYHFLLAKEYEKLGLWPQAIEEYRQFLPFFGV
                     SVPGYPDAHDYARKMVEFNDSPKDWTYPDLNDLVARIRKAIAARSASELRKYMSKAGF
                     FAMSWYKEGGEESNSNVLFNFSNFMTRGPIYVAPALDPSFNDSEAFLRTSGWTGLIPV
                     WYLCFQKVNFPADPNVHGNWEWAGIYFGEKMQ"
     gene            6171..7610
                     /locus_tag="PLS88_04980"
     CDS             6171..7610
                     /locus_tag="PLS88_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU06731.1"
                     /translation="MKNIRFIGILCLFYITLSLASAKDIEGANSFQEASEAISSISST
                     LEPSEEPPLEPPEELESTSLEETQEPLDLSQFLPEVPSVEELSLEDRAKSLEYDIPMP
                     WGHELFEKYRQYYLSNQGRKILLSTMEKAVPFMDYIQSRVTEYAVPQELAYLPVIESA
                     FSPFAVSKSGATGIWQFMSNSINGYGLTITEWVDERRDFMKSTDAALNKLMDNYKELK
                     DWPLAIAAYNAGLGSLRRAINAAGSNCDFWTIYDKGLVSRQALDYVPKFLAVASILRY
                     PDLYGLELPSPSIKWETISLEQQVDIKLLATKAEIPLETLKLGNAELHYTITPPENRY
                     LLKIPADKSDQVKAILADSNAPLVRYEIYKVKQGDTLTAISKRYGISIAMITRVNPGL
                     NPDKIRIGQTLMIPLSIQPLQSSSGSNSIAASGISIQTSIYTVQKGDTLYALARRYGT
                     TANVLASMNGIALNSILRIGQRLKVPARL"
     gene            complement(7615..8844)
                     /locus_tag="PLS88_04985"
     CDS             complement(7615..8844)
                     /locus_tag="PLS88_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOU06732.1"
                     /translation="MTELAKAFEFLEAQEKGIIELESLLTAYPALAPESGGIGEIEKA
                     AALEAWLKAQGISDIKHYDAPDPRVPSKLRPNLVATIPGKHAEGPLWIMSHLDVVPEG
                     ELSLWDSDPWKVVVKDSKLYGRGVEDNQQGLVSSVFAAIAFVKQGIIPERTIKLLFIA
                     DEEVGSKFGIQYLLENHSLFGKDDLIIVPDGGSVDGTEIEVAEKNICWLKITTKGKQT
                     HAAMPDKGANAFLAACDLTLRIHRLEKSTFTARDPLFSPDRTTINPTKKEANVPNINT
                     IPGEDVFYFDMRILPVYPVSMMLEEVSKQAHAIEAEYNVKIEFEVIQSNESRATPADA
                     PVVRILAEAIKRVYGVEARAIGIGGGTVGAYLRKAGFDCVVWSKMNETAHQPNENADI
                     ANIIGDAKVFAALAMAP"
     gene            complement(8891..9865)
                     /gene="galE"
                     /locus_tag="PLS88_04990"
     CDS             complement(8891..9865)
                     /gene="galE"
                     /locus_tag="PLS88_04990"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOU06733.1"
                     /translation="MKILVIGGAGYIGSHVARMLADKGNKVSIIDNLSTGTEENLFAD
                     YDFYKGDILDYSWLVSIIKHGFDAIVHLAAAKAAGESMIRPEKYATQNLTGTINILNS
                     VVEAGIPLIVFSSSAAIYGEPKYLPIDEAHPKEPTNFYGFTKLEIEHLLEWYDRLKGI
                     KFAALRYFNAAGYDIEGRIQGLEKNPANLVPVIMEVAAGMRPRLEIFGTDYPTPDGTG
                     VRDYIHVTDLAEAHCAALSHLAEARQSFAVNLGSERGLSVLEILQAARRITGRSIPVS
                     TVGRRPGDPAKLIASSAAAKRLLGWKAKYSDVDTIIATSWRVYEKHLA"
     gene            9995..10855
                     /gene="nadE"
                     /locus_tag="PLS88_04995"
     CDS             9995..10855
                     /gene="nadE"
                     /locus_tag="PLS88_04995"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOU06734.1"
                     /translation="MPVEINLSNEKERIVEFIRAQIAKYEFEGAVIGISGGVDSAVVG
                     KLLTEALGREKVFGLILPERDSSRRTVPDSLFVCRSLGIHYSVKNISSLVRSLGIYRT
                     KPPAFLFPRKIQENYAKALWLKTSTPYINDLKSAGGQSNRKNLAYYRTKNRFRMIALY
                     YEAEQRGYAVVGTINRTELLTGFYVKWGDDSSDIEPIVHLYKTQVFELAKELRIPEKI
                     IEKEPSPDLAPGISDEFAIGLCYFDLDRILEKIEEGQPLSGEDPELVEKVQQILAAVP
                     YRSIRNLNLL"
     gene            complement(10969..11041)
                     /locus_tag="PLS88_05000"
     tRNA            complement(10969..11041)
                     /locus_tag="PLS88_05000"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11006..11008),aa:Phe,seq:gaa)
     gene            complement(11059..11132)
                     /locus_tag="PLS88_05005"
     tRNA            complement(11059..11132)
                     /locus_tag="PLS88_05005"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11096..11098),aa:Val,seq:gac)
     gene            11257..11994
                     /locus_tag="PLS88_05010"
     CDS             11257..11994
                     /locus_tag="PLS88_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06735.1"
                     /translation="MYQRHSCLKPIIAAIFLILIATTATAFTFMPMSTTISPSGANSI
                     ASFRITNDSSQQIAITIKATTREIDENGNEINAPADNQFAIFPTRIVVQPNSFQNIKV
                     QYKGNPSLAKEVAYRIIAEQVPIDFSQQQTSGVKVMFRYIAALYVAPPNVSHKIAVTK
                     VEYGEQEGKKGFFVTITNSGTRHGLINDPVLKISGSLSTITLKDEALNAINGQNLLPG
                     NVRKFFIPCEDAVPGKSYTGSLTAKIE"
     gene            12070..>14065
                     /locus_tag="PLS88_05015"
     CDS             12070..>14065
                     /locus_tag="PLS88_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HOU06736.1"
                     /translation="MLKRKKVGILLFILIIELSSVLNAQIQETPIIREAVIPELEIQF
                     SISINGKPIGDIDTGIWHNEPILKKEVASRLLINYLRPDIYSVIFDTLFDELDWLTAK
                     DFSIAGISFEFSELLLSLSITIPPEYSPVIDIDFVPEQFQNYKPIIRPAPFAGFIQNE
                     SNFQVSDFSDGTALFSTRLNSMIDIKGAHLFADALLSYAFGSSQGFSWNLDSAYVLRD
                     STAKHSRAKIGKINIPGVSNQSQPSLYAMSLENLEDRQYIVRTGYIDNQTEFSIRKTA
                     RVSVEVNGKPIRQVLLEPGNYRILDLPFTTGLNEFVLRIEEIDGNVQVFRRTIPREAN
                     IMQPGTSRYALSAGTSHIDFSEFLASGYYLYGFSPTFSGGINLQVDKRSAMGGLTFST
                     ALPIGILNGSSSIVGRWDGWGHVISPAATLSYLFIIPSKTYVPTWGISATYRGEGFFP
                     PNVSSPTGTVPNALLSLSTNLYTKIFGNAGLGLSYSLYYTMSSPAKITQDVFASISTT
                     IAPGGNLSISGRLSFPESSKPAFSMNLSFIIIPKGDKPRILSFMQSSGGQTNIGISDK
                     LYMLGKVFDTNFGATNLLLGSSNDSSMSLGIRNSSEYFDVSANGNFSYSPSSASYSLG
                     GNAQFRSTMGFAGTHIAFTRQIPDSFLLITASPELRGKTVL"
     assembly_gap    14066..14165
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14166..14678
                     /locus_tag="PLS88_05020"
     CDS             <14166..14678
                     /locus_tag="PLS88_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU06737.1"
                     /translation="DSFLLITASPELRGKTVLYKINNGTQYLEMRGRNVIIPIQSYET
                     AILSTDLVGTELNISPRYSFVTISPAFRSGILFESDIIKQYIITGRLINQESKPITYL
                     PGDVFDLGGSLVTSTFTDELGKFEIYDILPGSYQIRWPEGYGISQVELPETEEDRIDL
                     GDIMLHIESK"
     gene            14705..15622
                     /locus_tag="PLS88_05025"
     CDS             14705..15622
                     /locus_tag="PLS88_05025"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HOU06738.1"
                     /translation="MRDRILLLSLLCFAIAFSANAEKLSLDPISPPNPVIYDPFSGND
                     TIGQSFTFTVRMDKSTGTTSNFSVVINGESSPSSRSLKSSSGSLEIGFYKDSSHTTEI
                     RSTANYSPSYYISGSFDPKPDVLTQTFTIYPRLVKGQSAPYGTYTETFTLVLYKKSTP
                     GQGSPEDTLSFTYTAEVGKAVDVRVGPPNASYDTGMNSYNIDLGEISKGASANFGIFI
                     KGTTGYTLTMHTASGGFLTSSNSSDKIQYTIVVDGKEYYLNSSVILDRETVDSLYSKA
                     LLGTVKIAADQNAEPGIYSDAVSFSVTVN"
     gene            15842..>16164
                     /locus_tag="PLS88_05030"
     CDS             15842..>16164
                     /locus_tag="PLS88_05030"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOU06739.1"
                     /translation="MNISRSSGVLLHPLSLGGPWPCGTMGDEARLFVDFCTQARLGWW
                     QILPLGPTAFGDSPYQSPSAFAGNPYLIDPQALAAQGLLSREELESYRCTRGKESVPS
                     LTRSK"
     assembly_gap    16165..16264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16265..17338
                     /gene="malQ"
                     /locus_tag="PLS88_05035"
     CDS             <16265..17338
                     /gene="malQ"
                     /locus_tag="PLS88_05035"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOU06740.1"
                     /translation="DYALFTAIKEKEQYRPWYEWPQKLKSREHSALEEFEKGNKELIC
                     RLKFTQFLFFEQWLALKQYANSKGLKLIGDIPIFAAWDSADVWAHRELFQLDEKGRPI
                     RVAGVPPDYFTATGQLWGNPLYDWQAHEQEGYAWWIARVQHSLKLVDALRIDHFRGFE
                     AYWAVPFGEPTAENGHWEKGPGHRLFNALKNALGELPIIAEDLGFITPEVIELRDSLG
                     FPGMRILQFAFELEEDNQDYPHNYPKHCIAYTGTHDNDTAMGWLAHAPLAAKKRALSY
                     MHAKPSTFYQDIVRTVWASPAMLAIAPMQDFLGLGTEARMNFPGTTSSWWRWRMNNES
                     LKPALAHRIKRLSEVYFRVSSSS"
     gene            complement(17557..17629)
                     /locus_tag="PLS88_05040"
     tRNA            complement(17557..17629)
                     /locus_tag="PLS88_05040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17594..17596),aa:Lys,seq:ttt)
     gene            complement(17797..18963)
                     /locus_tag="PLS88_05045"
     CDS             complement(17797..18963)
                     /locus_tag="PLS88_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06741.1"
                     /translation="MNLRQKTLAATILLIAFGSITVFAQTSLTNLLPKNARYMALGGS
                     SLVFTEGCDALWGNPAGLARKKSLTLFDSSTWTYIMPTPLNIQNLAAILNKQKNQEEI
                     TSILDGLVSQNGLGGGEYLQFGWISDGFGIGIDSVTDAIATGSGLDDSVLNARTQINA
                     VFGMGWLTHLGPFEINIGANVRGFYRMETSLDGWAFGPLSKAVTSKADLYSIIQSDRL
                     IGGFGFAIDAGGTLSIGPFSLGFMVRDLADRFAMKESSIKEIADTYMVPMGGLDFYAI
                     SPIYTVGLSLALDQDSLLPVTLFVEADDPLSIFPLFSSNIKEIPSKMHFGIEIGVLKF
                     SALRIGFNQGCFSAGFGIHLLFLDLNAAMFTEPVNIAGSKIKRSGVMLQGAIKF"
     gene            complement(19181..>19474)
                     /locus_tag="PLS88_05050"
     CDS             complement(19181..>19474)
                     /locus_tag="PLS88_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06742.1"
                     /translation="KFEPSLYTELENPQKIFSSSDMVASALNSLHAGAQVRLLRFITV
                     RGGLNKGWYSLGAGIDLSFLELDAAIFTEELGLYPGDKGRSGLSVQVGIRFGR"
BASE COUNT         5243 a         4560 c         4143 g         5328 t
ORIGIN      
        1 cttgccaaat ttgacctgta gtattcgctt gtaacatttt gccgcgaatt tggctcttat
       61 tctaagagcc cgtgatacat ttttgttatt gattctttta gttcactttt gaaggattag
      121 acaatgaaaa gaaaaacaaa agctatctta tttttagcgc taggaatagc aattctaact
      181 gctgggtgta cgattgtagt gaccccagct ggtgagcaat gtagcctgta ctatacatgg
      241 gaaggctcgc aagaaaagta tgcagtatgg acgctctatt ccaatggtac ctttaaggat
      301 aattaccaag ggagaggaat ttggagcgcg gatggtagtt acttctattt ggcctacaac
      361 aattccagct atttttatca gggaacagtt tatacaggga ctatctatag ccctgactat
      421 atgaatggag ttatgtcagg cccggataaa tatggaattg aacaatatgg catatgggaa
      481 gcatatcgag gagtacatgg gtatagagcg cttacgccct cttctgaaaa agatcccggc
      541 tctaaaacct tgtcaccttc cggcgaaccg atcgaaaatc atgagaatga ggagacaaat
      601 gaatgaaatt gcataggaaa atagctcttg caagcttgat cctgcttgca ttcgccagct
      661 atacatttgc gcaggtcaag gtgtcgctca ctatacaggc aaatgtccaa aacgcgcagg
      721 tctatatcga cgataatctt gctggctatg cctcgcccaa tctctccttg ctgatttttc
      781 caggcactta taccatcaga gtggtaaaga acggatacca ggaatataga acaacgatta
      841 ccgcagcagc aacgccaatc actctcttcg caactctgtc aggaggagcc ccgcagactc
      901 catctacgcc acccgctcca tcaacacctt caagcccctc aacaccaccc tctccacccc
      961 cttataccct gccactaccc atcactattc cggagcctgg ctttcagctt tctatcgatg
     1021 ccaatatcga tggagcaagg gtctatatcg acaatgttta tgtaggtata actccttacc
     1081 gcgtaacaca atatcgcgga ggccatgtga tccgtatctc cgctccgggc tatgctgatt
     1141 atgtcgagag cttgtacctc tatcgctcta cggtgcttaa cgcttacctc tctcctctgc
     1201 ccgtttctta tgaaataagg cttcccgata atctacagct cgagtggaga acaaacaata
     1261 agtaccatga taaattccag gccgtgcaac tttacctaga taacaagcga ctcgacagcc
     1321 ttcgcggaga attgttgcct gggctgcaca ccatcgctat agactattac aacctgcatc
     1381 tagaagattc attcgatata aagccaggcc aacccgttat cttgcagctt ggcctgggcg
     1441 ttaaggtata ttgaatcagc tttgaatcga gccctggctt aggcgctaca gcatgcaact
     1501 agaaagcgat tctatacaaa actatactct gctaactaca tataccgcaa taaaaaaggc
     1561 acattattgc aatgcaataa tgtgcctctg atcgggcaag ccagactcga actggcgacc
     1621 ccaagtcccc cagacttgta cgctaaccac ctgcgctatt gcccgatttg cctctcaggg
     1681 ctcaaataaa ctaacacaag ccgcatccag ttgtcaatgt gaaagtggct cctagggtgg
     1741 gctcatagac aaagaaagcg ctcgtcgcca gggtgcggat cgattcccgg cctggtcaag
     1801 gcaaagtcat acttgactgg atcttcgggc gcatagagtc taaacgcgtc ggtaacgcgt
     1861 agcgcatcgc gaaggttcac cgccactccg cttttcctct ggattttcct ctgtggcaaa
     1921 aaacccaatc gctctgtgca agtgcggatc atatgtgtat ccattggaac aatgagacgt
     1981 gaaggactca cgctattcca gcagcctgga tctatttcat cgcgccttac catccagcgc
     2041 agaaacaaaa aaagcctctt tgccgccgaa ccattggccg gatcgggcag aagattcttg
     2101 cgcaagcctt cgggtacagc ttcgagcgcc aactcatgca ttcttcgaac aaacatagat
     2161 gaagcagcca agatgttctg ctgctgactc attttgcatg agagggattc gtgccgcccg
     2221 gtacatattt cgtacttgct gacttcctgc tgattcgcag caaaatcaca agcttcttcc
     2281 ggtttttcct gcagccattt tctatcacat tccaaaaaga gcgcttctag gcttccatag
     2341 caattcagtg catcatgaat ggcccacata agggcaatca tatcctttgg aaaacagaat
     2401 cgatatttaa aagaagacca tgctagctct atctccttgc ttgccatctc gcgcaatgtt
     2461 tttgctggat gctctcctaa cggctcgagc gctttttcgc aagctcgcat aataagctcg
     2521 acagatccaa atgcaagtgt cgaacatatg agtccagcta cttccatgtc ttcacgcgcc
     2581 ttgtagcgct tcacaatagc gagcggatcg agcttaaggc gctcggatcg attgcattct
     2641 gcgtatatct cttcgagaaa acatgaaatt gctggattat aaagaggtaa ttgctggtcg
     2701 gaatttttcg agctgtttaa ttgccagctg accttttcta ggttgtattt tggcttgctt
     2761 tttaatcttt cgttgtttaa ttgccagctg acctttttcg gtctttcgga tgaactgctc
     2821 ttcataattt tgctatggat acctgaacat gtttccgcga gctctatcta gaagaataac
     2881 cgagctctgc ttccagctgc tttatgcgcg ctagaatctc atcatcattc ggatgaaggg
     2941 atttagcttg ttcaaggtat agaacagcct tatcgcgatt tgtggtatcg gacttgtcag
     3001 attgccgatt atagcgattt atgtatattg aagcgagcga gacaagcgct tccacattgt
     3061 ccgctctttc tgctagtgcg tcttttagct gacttatagc tttgttttcg tcactctgaa
     3121 gcgtggctga catataatag aggaaagatt tcaactcttt cgaaaaaggt gcggctataa
     3181 atttcagaac tatatcgagt aatgccgagt ttgcgccttc atcttgactc atcccaagtc
     3241 caggcaaacc cggcacaata gccgtaggta catccctagt cggtgcagcc gaggccaggc
     3301 ctcctccagt tgccatagca agactgcgca aataggcttc aacggctttt tccgatgtcg
     3361 gttcctgcaa ataccagctc aaggcaaact gtaaggcttc ttggacattt cctgattttc
     3421 tcaggatgac agctaccatc tccttatcaa gtggaacacc tcctgcttct tggagatatt
     3481 tagatgccat aatccaatct tgattgttat agttttccat tacaaggaat tgaaaggctt
     3541 tgtcttctgc ttgtttcctt aatgcctgct ccagcggtgt cagattaagc acttcatttt
     3601 ttgttatatc tagtactttc tttgccaaat cagcagcttc cagatgtcgc tgcgcatcag
     3661 tcgactcctg aaggactcta acaagctgtg caagtgccac aggatcctgg ggataatagc
     3721 ttactgcagt cctggcaaaa cgctctgcat cggtttgacg cgaattctcc agagcatatt
     3781 ggctctttag gatgagccag gttctgtcct ttgacgcaat cttcgaagca ctttctagat
     3841 ttttccaggc tgaggcatag tctcgcttct gcattgctgc atgcgctatg ggaactaaca
     3901 aatccgtcct agatggatct tcgttgagcg cggctatgag ccagggctcg gcatcgagta
     3961 tgttgtttag attatataat gcaattccat ataatgattt aagatcggcg ctaaaagtat
     4021 cttgctctat ttctgttgtt tctatatatc cgcctccggc ggatactcct ttgtttgaag
     4081 aaggcttttt gttatatcca tctttggttg atgtttcttt agcggattca actagagcta
     4141 tagatattat aggatcgaga atttgctttg ctttttcggg tttaccttgc gctaggtatg
     4201 ctcgcgcaag accacaagct gacttctttg atagagtatc gagttcgagt gctttctgga
     4261 attcatcggc ggcttcggcg tattgctttg cctcgaaagc tatctgacct ctcacaagcg
     4321 atggaacagc tgaaattccg ccgaggtcct ctagtctaga aatagatgca agcgcttgtg
     4381 cccttatttc gttacgagag tcccgaaaga atggcaaagc agaaatgagt tcactcatca
     4441 gcgaacaact tccattctca aaaacctttg gagtctttcc tgcggaagcg tcggatagtc
     4501 cggccaaaca tgtgatatat ttttgatcgg ctccctttgc tgcagctgga atctcgaaga
     4561 cagcattttt tgggccatac acaataaggc caatgctttt tgcaattgca tctagtgctg
     4621 ctttgtctgg ttctgataga taaggggaag ctttggtctt agagcgcgcc tgagccaggg
     4681 aatgaggagt cgcaagcact atgagtgtct caatatcctt ggcttcagtt gagatttttt
     4741 taagctgctc tacatcggtc tttggcagat tttgctgtgc atagctccaa gtgggagccg
     4801 aaaaggacaa taccgataat gctgctatcg taaggagaaa tatatatttg attttcatcc
     4861 aataccaata tcggcattcc atggcaaatt ttaattttag cctaattgag ctcgtgctgc
     4921 aagaaaaatt tatgactaaa ttgctatgcg cccttagatc ttttgataat tatattaaca
     4981 tgtcgcatga ctcttgggtt ttgaacatct gcgctaatat gtcgtgcgac cagcgcttgc
     5041 ccagaaaaaa tgatgcatga taatgtatca agaatgaaaa gtgccaggat aggtgcattt
     5101 acagctccac tcctcatttc gattttaatc gtctttttca tgggattatg gggtcttaga
     5161 gtttccggga ttactttttc caatctaggc gaattttcga tatcgaataa atctctacga
     5221 agagagcttc gcgacgctct tcatgtcttt aattctcctc agttgaagaa tagtggctat
     5281 tactcgatcc agcgcatgct tgcagccaaa cgaatcagca ccattctgat caaagatggg
     5341 caaaaagcga gtgctgcaag atttctttct tctcttgcgg atgagcatga tgattatgaa
     5401 gcttggtatc ttttcgcagc gggggcaatc tacgagtcca tggatagtag tcctattgcc
     5461 cgcattttct atgagaggat tctcaagaat ctacccgata tgactataga tggccagtca
     5521 atccatagga tatctcttat caagctgctc gagggcgata gtccgcccga gaaaaaagca
     5581 cattggtatg gagaattcat tagacgcttt cccgaagccg aggaagtagg tccttaccat
     5641 tttcttcttg caaaagaata cgaaaagcta ggactttggc cgcaggctat cgaagaatat
     5701 cgccaattct tgcctttttt tggagtatct gttcctggat atcccgatgc ccacgattat
     5761 gcgcgcaaaa tggtcgaatt caacgatagc cccaaggatt ggacataccc cgatctcaac
     5821 gatcttgttg ctcgcataag aaaggcgatt gcagcacgtt cggcctcaga gctcagaaaa
     5881 tatatgtcca aggcagggtt ctttgcaatg tcctggtata aggaaggagg ggaagaatcc
     5941 aattccaatg ttcttttcaa ttttagcaat tttatgacca ggggccccat ttatgttgct
     6001 cccgccctag atccatcctt caatgactca gaagcctttc tccgcacgtc aggatggaca
     6061 ggcttgattc ccgtatggta tctgtgcttt caaaaagtca actttccagc cgatcccaat
     6121 gttcatggca actgggaatg ggctggtatc tactttggag aaaaaatgca atgaagaata
     6181 tcagatttat cggaatattg tgtctattct acataacgct atccttggca tcggcaaaag
     6241 atatcgaagg cgctaattct ttccaagaag ctagtgaagc tatttcgagc atttcatcta
     6301 cactcgagcc atcagaagag ccaccactcg aaccgccaga agagctcgag agtacttccc
     6361 ttgaagaaac acaagagcct ctcgacttga gccaattcct gccagaggtg ccaagcgtcg
     6421 aagaactcag cctagaggat agagcaaaga gtcttgaata tgatattccc atgccctggg
     6481 gtcatgagct tttcgagaaa tatcggcagt attacttgag taaccagggt cgcaaaattt
     6541 tgctctcgac catggaaaaa gccgtgccat ttatggatta tattcaatcc agagtgacag
     6601 aatatgctgt ccctcaagag ctggcatatc tgcctgtaat tgaatccgca ttctcgccct
     6661 ttgcggtttc gaagagcggt gcaactggaa tttggcaatt catgagtaat tctataaatg
     6721 gctatggact cacaataacc gaatgggtgg atgagcgccg cgattttatg aagagcacag
     6781 atgcagcact caacaagctt atggataatt acaaggaact caaggactgg ccgcttgcta
     6841 tagctgcata taatgcgggc ttgggttcgc tgaggcgcgc tataaatgcc gcaggctcaa
     6901 actgcgattt ttggacaatc tacgataaag gtttggtttc gcgacaggct ttggactatg
     6961 ttcctaagtt tctagcagtg gcatcgattc tgcgttatcc ggatctctat ggcttagagc
     7021 taccatcgcc cagcattaag tgggaaacta tttctctcga gcagcaggtg gacataaaac
     7081 ttcttgctac aaaggctgaa attccgctcg aaactctaaa gcttggaaat gccgaattgc
     7141 actatacaat cacaccgcca gaaaacagat accttctcaa gataccagcc gataagtcgg
     7201 accaggtaaa agccattctg gcggattcca atgcccctct tgtaagatac gaaatataca
     7261 aggttaagca aggcgacact cttacagcca tatcaaagcg ctacggtatt tccatagcaa
     7321 tgattacaag ggtcaatccc ggcctcaatc ccgataaaat aagaataggc cagaccctca
     7381 tgattcctct ctctatccag ccccttcagt cctctagcgg ttcgaacagc atcgccgctt
     7441 caggcataag tatccagact tcgatttaca ctgtacaaaa aggcgatact ctttatgctc
     7501 tcgctcgtcg ttatggaact actgccaatg ttttagccag catgaatgga attgcgctga
     7561 attcgatttt aagaatcggc caaagattaa aggtaccagc tcgtttatga ttttctaagg
     7621 agccatcgct agcgcggcaa agacttttgc atccccgatt atattcgcaa tatccgcgtt
     7681 ttcgttgggc tggtgtgccg tctcgttcat cttggaccat actacacaat caaaaccagc
     7741 cttgcgcaga taagcgccca ccgttccgcc tcctattccg attgcccttg cttcgactcc
     7801 atatacccgc tttatagcct ccgctagtat ccttaccacc ggagcatctg ctggcgtagc
     7861 cctcgattca ttcgactgaa tcacttcgaa ttctattttt acattgtatt cagcttctat
     7921 ggcatgggcc tgcttcgata cctcttcgag catcatagat actggatata cgggtagaat
     7981 gcgcatatcg aaatagaaaa cgtcctcccc tggaattgtg ttgatattgg gaacatttgc
     8041 ttcttttttg gtgggattta tggttgtgcg atctggtgaa aacagaggat ctctagcggt
     8101 aaaagtggat ttttccagcc tgtggatgcg cagtgtaagg tcacaagcag caagaaatgc
     8161 attagcgccc ttgtccggca ttgcggcatg ggtttgtttt ccctttgtcg ttattttaag
     8221 ccagcagata ttcttctcgg ctacctcgat ttctgttcca tcgaccgagc caccatcggg
     8281 aactattatg agatcatctt ttccaaaaag gctatgattt tcaagaaggt attggatgcc
     8341 aaatttggaa ccgacctctt catcggctat gaacaagagc tttatagtgc gctccggaat
     8401 aataccctgt tttacgaatg ctattgcagc aaatactgat gatacaaggc cttgctgatt
     8461 gtcttctacg cctctgccat agagcttgct atccttaacg accaccttcc aggggtcact
     8521 atcccataag cttagttcgc cctccggcac gacatccagg tgagacatga tccaaagcgg
     8581 gccttctgcg tgttttccag gaatcgtcgc taccagattt gggcgcagtt tcgaaggaac
     8641 acgtggatcg ggcgcatcgt agtgcttaat atctgaaatt ccttgtgcct tgagccacgc
     8701 ttctagggca gcggctttct caatctctcc aatgccccct gattcgggag cgagcgcggg
     8761 gtaagcagtg aggagactct ccaattcgat aatgcccttc tcctgagcct ccaagaattc
     8821 aaatgcctta gcaagttctg tcatggtctt ttcctttcct ttccaagcag atttgaatca
     8881 atcgaaatgc ctatgccaaa tgcttttcgt agacgcgcca cgacgtggcg ataattgtat
     8941 ctacatcaga gtattttgct ttccatccca agagtcgttt ggcagctgcg ctggacgcga
     9001 tcagtttagc cgggtctcct ggcctccgcc ctacagtcga aactggaatc gaacgaccag
     9061 tgattcgccg tgcagcttgc agaatttcca gcacagatag gcctctttcc gaacctaggt
     9121 ttactgcaaa gctctgcctt gcttcagcca gatggctcaa agctgcacaa tgggcttcgg
     9181 caagatccgt tacatggata taatcgcgca cgcctgtgcc atcgggtgta ggataatcgg
     9241 taccgaatat ttcgagcctt ggcctcattc ccgcggccac ttccattatg acgggcacaa
     9301 gattggctgg atttttctct agaccttgta tcctgccttc gatatcgtaa ccagctgcat
     9361 tgaaatagcg cagcgccgcg aatttgatcc cctttagcct atcataccat tcgagaagat
     9421 gctcgatttc gagctttgta aaaccataga aatttgttgg ttccttggga tgagcttcat
     9481 ctattggtag atatttgggc tccccataga tggctgccga agaagaaaac actattaaag
     9541 gaatccccgc ttcaactacg gaattgagaa tgtttatcgt tccagtcaag ttctgagtcg
     9601 cgtatttttc gggtcggatc atagactcgc ccgcagcctt tgccgcagca agatgcacta
     9661 ttgcatcgaa tccatgcttt attatggaaa ctagccaaga ataatccaga atatcgccct
     9721 tgtagaaatc ataatcggca aaaagattct cttccgtgcc ggtagatagg ttatctatga
     9781 tgctgacctt gttgccctta tcggcaagca ttctcgcaac atgactcccg atataccctg
     9841 ccccaccgat caccagtatc ttcatgcgat tttcctcctt ggcaaatctc tacagcattc
     9901 atgctatatt tgaatatatc ggcggcgtca atatggcaac tctttattcg actcgcctat
     9961 aaaggcatta ttgtagatat aaaagaggct ttttatgcca gtagaaatca atctgtccaa
    10021 tgaaaaggaa agaatcgtag aattcatcag agcgcagatt gccaagtatg aattcgaagg
    10081 agccgtgata ggcatcagcg ggggagtcga ttctgccgtc gtcggcaaac tcttgacaga
    10141 agccctgggt cgggaaaagg tctttggtct cattttgcca gaacgagatt catcaagaag
    10201 gacagtcccg gattctctgt ttgtatgcag aagtcttggc attcattatt cggtaaagaa
    10261 catttcgtcc cttgtcagaa gtcttggaat ctataggaca aaacctccgg cttttctttt
    10321 cccgagaaaa attcaggaaa actatgctaa ggctctatgg ctaaagacca gcacgcccta
    10381 tatcaacgat cttaaaagtg caggtggaca atcgaacaga aaaaatcttg catactatag
    10441 aactaaaaat cgattcagaa tgatagctct ctattatgaa gcagaacaga gaggttatgc
    10501 ggttgttgga actatcaaca gaacagagct tcttacaggt ttctatgtca agtggggaga
    10561 tgattcgagc gatatagaac ccatagtgca tctctataag acacaggtat tcgaactggc
    10621 aaaagagctc cgcattcctg aaaaaatcat cgaaaaagaa cccagcccag atttagctcc
    10681 aggcataagc gatgagtttg ctattgggtt atgctatttc gatttggata ggatacttga
    10741 gaaaatcgaa gaggggcagc cgctctcggg cgaagatccg gagcttgtcg aaaaagtaca
    10801 gcagattctt gcagccgtac cttatcgaag tattagaaac ctgaacctat tatgaaaaga
    10861 agaccgactc tccctctcga tgcagcctat acaccctatc gcgagcatcc agcgtagatg
    10921 tcttgcctct ggcgcacaaa aaagccacct cataatgagg tggctctatg ccgccgatca
    10981 gaatcgaact gatgacacat ggattttcag tccattgctc taccaactga gctacagcgg
    11041 cctatcatat aaaggcattg ggcggtgcaa gagtcgaact tgcgaccccc agcgtgtcat
    11101 actggtgctc taaccaactg agctaaccgc ccgctccttg ccgcgaacga cagaagctct
    11161 tataccagca gataaagaga ttgtcaagcg accccagaat gcaattgcat ttatcctctt
    11221 ttgcatatag aataataaca atagcgggag gaacgcatgt atcaaagaca ttcttgtctc
    11281 aagcctatta tagccgccat ctttttaatc ctaatcgcaa caacagcaac agcatttact
    11341 ttcatgccca tgtcgaccac catctcgcct agcggtgcaa atagcattgc aagttttcga
    11401 atcacaaatg atagttcaca gcaaatcgct ataaccataa aagcaacaac tcgcgaaata
    11461 gatgaaaatg gtaatgaaat caatgcgccc gccgacaatc aattcgctat ttttccaact
    11521 cggattgtgg tacagcccaa ctccttccag aatatcaagg tacaatacaa aggaaaccca
    11581 tccctagcaa aagaagtcgc ttatcgcatt atcgcagagc aggtccccat cgactttagc
    11641 caacaacaga cttctggcgt caaggtgatg tttcgatata tcgcagcgct ttatgtagct
    11701 ccgccaaatg tcagccataa gatagctgtt acaaaggttg agtatggaga acaagaaggc
    11761 aagaagggtt tctttgtaac aatcaccaat agcggtactc gccatggtct tatcaacgat
    11821 cctgtattga agatatctgg ttccttatcg acaatcacgc ttaaagatga agccctaaat
    11881 gcaatcaatg gtcagaattt gctacctggc aatgtgcgaa aattcttcat tccatgcgag
    11941 gacgcggttc ctggcaaaag ttatacaggt tcgctgaccg caaaaatcga atgaatcatc
    12001 tatgcactct tgaatgagca tgaaagcgct gagccttaag gcttatttag aggcccaagc
    12061 gagtatgaaa tgttgaaaag aaaaaaagta ggcattcttt tatttattct aattattgag
    12121 ctttcctctg tattgaacgc acaaatccag gagacaccga ttatccgcga ggctgtcata
    12181 ccagagctag agattcaatt ctctatcagc ataaatggca aaccgatagg agatatagat
    12241 acaggaatat ggcataacga accaattctc aaaaaagaag tggcttcgcg gctgctcata
    12301 aattaccttc ggcctgatat atactcggta atcttcgata cactattcga cgagctcgat
    12361 tggctcactg ccaaggattt ttctattgct ggcatttcct ttgaatttag cgaattactg
    12421 cttagccttt ctattaccat tccgccagaa tatagccctg ttatagacat cgactttgtg
    12481 cctgaacaat ttcagaatta taagcctatt atccgaccag cgccttttgc tggctttatc
    12541 cagaatgaat cgaacttcca ggtatccgat ttctcagatg gcacggccct cttttctaca
    12601 cgcctcaact cgatgatcga tataaaggga gctcatctct ttgcagatgc tcttctatca
    12661 tatgcttttg ggagttctca aggtttttct tggaatctgg attcggcgta tgttttacgg
    12721 gacagcaccg ccaaacattc aagagcaaaa attggaaaga tcaacattcc tggagtcagc
    12781 aatcaatctc agccctcgct ttatgccatg tctttagaaa acctcgaaga tagacaatat
    12841 atagtgcgca cgggatatat agacaatcaa acagaatttt ctatacgaaa gactgcaaga
    12901 gtatccgtcg aagtgaatgg aaaacctatt cgccaagttc tgctcgaacc tggcaattat
    12961 cgaattctcg atctgccatt cactactggg ctgaatgaat ttgtgctgcg cattgaagag
    13021 atcgatggaa atgtccaggt attccgccgc acaattccaa gagaagccaa tataatgcaa
    13081 ccaggaacat cgaggtatgc tctttccgca ggaacatcgc atattgactt cagtgaattt
    13141 cttgcatctg gttattatct gtatggcttt tcacctactt tttcaggtgg catcaatctt
    13201 caagtcgaca agcgctctgc aatgggaggc ttgacttttt ccacagcact gccaatcggc
    13261 atattgaatg gttccagctc tattgtaggg cgatgggatg gttggggcca tgttatttcc
    13321 ccagcagcaa cgctaagtta tctctttatt ataccttcaa aaacctacgt gcccacatgg
    13381 ggaatctctg ctacatatcg aggagaagga ttctttccgc ccaatgtatc atctcctact
    13441 ggaacggttc ctaatgcttt gctctctcta tccaccaatc tctatacgaa aatcttcgga
    13501 aatgccggtt tgggattgag ttattctctc tactacacta tgtcctcgcc tgccaaaatc
    13561 acgcaggatg tctttgcttc aattagcact acaattgcac caggcggaaa tctgagcatt
    13621 tccggacggc tttctttccc cgaatctagc aagcctgctt tttcgatgaa tctatccttt
    13681 atcataatcc ccaaaggcga taagccgaga attctaagtt tcatgcagag ttctggagga
    13741 cagacgaaca taggtatctc agataagctt tacatgcttg gcaaggtttt cgatactaac
    13801 ttcggcgcta ctaatctact tctcggttcg tctaatgata gttcaatgag tcttggaata
    13861 cgaaattcat ctgaatattt cgatgtaagc gcaaatggaa attttagcta ttctcctagc
    13921 tctgcaagct attctttggg tggaaacgcc caatttagaa gcaccatggg ctttgctgga
    13981 actcatattg catttacgcg ccagatacct gattccttcc tcttgatcac ggcatcgccg
    14041 gagctaagag gcaaaactgt tctttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnngattc cttcctcttg atcacggcat cgccggagct aagaggcaaa actgttcttt
    14221 ataaaatcaa caatggaact caatatttgg agatgcgcgg aagaaatgtc atcattccta
    14281 ttcaaagtta cgaaaccgcc atactttcga cagaccttgt gggaactgaa ttgaatattt
    14341 ctccgcgcta ttcctttgtt acaatctcac ccgctttccg gagtggaatt ctcttcgaga
    14401 gcgacattat caagcaatat atcattactg gtagacttat caatcaagaa agcaaaccaa
    14461 ttacgtatct gccgggcgat gtcttcgatc ttggaggctc gcttgtgact tctaccttta
    14521 ccgatgaact aggcaagttc gagatctatg atattcttcc tggaagctac caaatacgat
    14581 ggccggaagg atatggaata agtcaggtcg aattgcccga gaccgaagaa gacagaatag
    14641 acttgggaga tataatgcta cacatcgaga gcaaatagta ctataataga aaaggaatat
    14701 aaatatgaga gatagaattc tgctgctttc gctattatgc tttgctatcg cattttcggc
    14761 gaatgcagaa aaattgagcc ttgatcctat ttcgcctccg aaccctgtca tctatgatcc
    14821 tttttctggg aacgatacta ttggccaatc attcaccttt acagtgcgaa tggataaaag
    14881 cacaggcacc acaagcaatt tttcagttgt cataaatgga gaatcatctc cctcttcaag
    14941 aagtcttaaa tcatcttcgg gctctttaga aataggcttc tacaaagatt cctctcatac
    15001 aaccgaaatc agaagcacgg caaattattc gccttcttat tatatctcag gatccttcga
    15061 tccaaagccc gatgttctta ctcaaacctt tacaatatat cctcgcttgg tcaaaggcca
    15121 aagcgcgcca tatggcactt atactgagac ttttactcta gtactttata aaaagagtac
    15181 gccaggacag ggtagtccgg aagatacttt aagtttcact tacacggcag aagtcggcaa
    15241 agcagtagac gtgcgcgttg gtccgccaaa tgccagttac gatactggaa tgaattctta
    15301 taatattgat ctgggcgaga tatcaaaggg tgcaagcgct aatttcggca tttttatcaa
    15361 aggaacaact ggatataccc tgacaatgca tacggcctct ggtggatttc ttacgtccag
    15421 caattccagc gacaagatcc aatacactat agtggtcgat ggcaaagaat attatcttaa
    15481 ttcaagcgta attctcgatc gagaaacagt tgattctctt tatagcaaag cattattggg
    15541 cactgttaag atagccgccg accagaacgc cgaacctgga atatattcgg acgctgtaag
    15601 cttttccgta acggtaaact gagcttgctg agtcgaacca gcttaagtga gctatataaa
    15661 gcgagcttat taggccgacc gaggaactaa gtcttttata ttttctcaat agcacacatg
    15721 aaagacctct gctctgctag ttaagaatca aaagtgcact tttgattctt aaatgacaat
    15781 aagggcctct actcttgaca gaatcatatt tgtagcgtac atcatgtaaa tcggtttaat
    15841 tatgaatatc tcgcgaagca gcggtgttct cctccatcct ctttccttgg gtggcccatg
    15901 gccttgcggg accatgggtg atgaggctcg cctctttgtg gatttttgca cgcaagcgcg
    15961 cctgggttgg tggcagattc ttcctctggg ccccacggcc ttcggagatt cgccctacca
    16021 gagcccatcg gcatttgcag gaaatcctta tttaatcgat cctcaggcac ttgcggctca
    16081 gggcttgcta tccagagaag agctcgaatc ctatcgatgc acaagaggaa aggaaagcgt
    16141 gccttcttta actcgctcca agcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnngattat gcgcttttta cagcaatcaa agaaaaagaa cagtatcgac catggtatga
    16321 atggccacag aaactcaagt ctagggagca tagtgctctt gaggagtttg aaaaaggcaa
    16381 taaggagctt atctgccgcc taaaatttac ccaattctta ttctttgagc agtggctggc
    16441 gctcaagcaa tatgccaata gcaagggctt gaagctgata ggcgatattc ccatctttgc
    16501 cgcctgggat tcggccgatg tatgggcgca ccgcgaacta ttccagctcg atgaaaaggg
    16561 aaggccaatc agggtagcag gtgttcctcc tgactatttt acagcaaccg gtcagctctg
    16621 gggaaatccg ctctatgatt ggcaagcgca cgagcaagaa ggctatgcat ggtggatagc
    16681 acgcgtgcaa cattcgctaa agctcgttga tgcccttaga atagaccact ttcgaggatt
    16741 cgaggcatac tgggccgttc catttggaga gcctaccgcg gaaaatgggc actgggagaa
    16801 aggccctggg catagactat tcaacgcgct gaaaaacgcg ctcggagaat tgcctatcat
    16861 tgcagaagat ctaggtttta tcaccccgga agtaatcgag ctgcgcgata gtctgggatt
    16921 tccaggcatg agaattctac agtttgcctt tgagctggaa gaagataatc aggattatcc
    16981 tcataattat ccaaaacact gcattgccta cacaggcacc cacgataacg acactgcaat
    17041 gggctggctt gctcatgcac ctcttgccgc aaaaaagcgc gcactctctt atatgcacgc
    17101 aaagccaagc acattctacc aggatatagt gcgcacagta tgggcaagcc cagctatgct
    17161 cgccatcgct ccaatgcagg attttcttgg ccttggcaca gaggcgcgca tgaattttcc
    17221 aggaaccacc agcagctggt ggcgctggcg catgaacaat gaaagcctta aacctgcgct
    17281 agctcacaga ataaaaaggc tatcggaagt ttattttagg gttagttcgt cgagctgaaa
    17341 agctagccgc atctctaaat aatttccatc aggatcttac taggacttgc ttgaaaacca
    17401 tttagcacaa ggccttatgc ttaatgtaag ctgggagctg gcatactata tctatgtgtt
    17461 caatattggt aaacactgat tctgcataaa ttatcattat gagagataaa acgctaccat
    17521 aagattataa gaaaacaact tatattttaa ttcttttgag ccgccccagg attgaactgg
    17581 ggacccacgc cttaaaaggg cgttgctctt ccaactgagc tagcggcccg cgttcatagt
    17641 gcgcttcgtg tgtctagtgc aatttgtgca cattgtgcgc ctttcgcggg ttgtaacact
    17701 agcgaaaacc gcatgaaatg tcaaggttcc gcatttgctt gatcgctttt gcacagattt
    17761 gtactgcacc ctacaaattg ctgaattctt ttttggtcag aacttgattg ctccctgcag
    17821 cataacgccg ctgcgcttga ttttgctgcc cgctatattc actggctcgg taaacattgc
    17881 agcattcaaa tccaagaaga gcaaatggat accgaatcca gcagaaaaac aaccttgatt
    17941 gaagccaatg cgcagcgcag aaaatttcaa tacccctatt tcaatgccaa aatgcatctt
    18001 tgaaggtatt tctttgatat tgctggaaaa tagtggaaag atcgatagag gatcatcggc
    18061 ttctacaaat agcgtaactg gtaaaagaga atcctgatcg agggcaagcg aaagaccaac
    18121 agtatagatc ggcgaaattg catagaagtc gagccctccc atgggaacca tataggtatc
    18181 ggcaatctct ttgatgctcg attccttcat ggcgaatctg tctgcaagat ccctcaccat
    18241 aaaaccgaga gagaagggtc caatggagag agttccacca gcatctatcg caaagccaaa
    18301 tcctcctatc agtctatccg attggataat ggaataaaga tcggccttcg atgttacggc
    18361 tttcgaaaga ggtccaaaag cccatccatc taagcttgtc tccatcctat agaaaccacg
    18421 cacatttgcc ccaatgttta tctcaaaggg tccaagatgt gtaagccaac ccattccaaa
    18481 aaccgcattt atttgagtcc tcgcattcag gacagaatcg tcaagcccgc tacctgttgc
    18541 tatggcatct gtcactgagt cgatacctat gccaaaacca tcgctgatcc agccaaattg
    18601 aaggtattcg ccgcctccta atccattctg tgaaacaagt ccatcgagaa tagatgtgat
    18661 ctcttcttgg tttttctgct tgtttaggat tgctgcgaga ttctgaatat tcagcggtgt
    18721 tggcattatg taagtccatg tagacgaatc gaaaagagtc aatgattttt tgcgcgctaa
    18781 tcccgctgga tttccccata gagcatcgca gccttcggta aacactagcg atgatccgcc
    18841 caatgccata tagcgcgcat tcttggggag tagattggtc aaactagtct gagcaaaaac
    18901 tgtaatcgag ccaaacgcaa taagcaaaat agtagctgct agagtctttt gacgcagatt
    18961 caaatgatca ttcctttgat ataaacctat atcttctctt agagtatcgg aacgaaatca
    19021 ttttaccata tgccgaattt cagaaaaaaa gccatcagct tttggaagcc ggtggctttt
    19081 ttggaagtct ttctctgaaa gtctcttctg actttttcag cctagtttat tcctgtcgta
    19141 ctctatcaac aataatcaag atcaggaata atgtttatta ttaacggcca aatctgatcc
    19201 caacttgcac cgatagaccg ctccttccct tgtccccagg atataaccca agttcttcgg
    19261 tgaagattgc tgcatctagc tccaaaaacg aaaggtctat accagctcct aggctgtacc
    19321 atcccttgtt gagcccgccc cgcacagtga taaagcgcaa gagtcgcact tgtgcacccg
    19381 catgtagtga gtttaatgca cttgcgacca tatcactgct agaaaagatc ttctgcggat
    19441 tttccagctc tgtatagagg cttggctcga actt
//
LOCUS       DAOSOB010000034        19261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_2851_length_19261_cov_77.559617, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000034 DAOSOB010000000
VERSION     DAOSOB010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 19261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19261
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2851_length_19261_cov_77.559617"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            477..1805
                     /gene="prs"
                     /locus_tag="PLS88_05055"
     CDS             477..1805
                     /gene="prs"
                     /locus_tag="PLS88_05055"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOU06743.1"
                     /translation="MSYSDPTHLGIIACPGAEAFANQVIRKLSGIYKHRFMRKAQAIA
                     GRYHITPDLVIRKINFANDCNTGMLFLPGDKERYRPPKFKIDVRHTFFANGEIKTEIL
                     ESIRGRSIYIFQDVENHQPVSFNDGTIQKVLSVNDHIFNLFVAIDAAMQAGAAEISLV
                     LPIYPYSRQHKKKGREGLTAARIGKMLESLGVDRIITLDIHSKEIENAFDRLRMENLH
                     ASFQIIEKLTSIIDIAKEDIVVLAPDTGAVDRNKFYANALQKPLALLYKERDYSKITK
                     SAAETNISEMRLLGNVSNKVVFMADDMIGTGGTLIKAMRYLKELGASKVICAVSLPIF
                     SANAIDEFDAAYKEGLFYRVIGTNAIYHNELLKKEWYIQSDVTGLFAQVISLLHHDRS
                     LSSLLDNRDVVARLIKKKLESTGSSRVSSNQPKDENISEDVIPYGAANKP"
     gene            complement(1858..2883)
                     /locus_tag="PLS88_05060"
     CDS             complement(1858..2883)
                     /locus_tag="PLS88_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOU06744.1"
                     /translation="MSIATFGEIMLRLKSPGQERLLQSPFFEATFGGSEANVAVALSR
                     LGIPARFISILPDNPIGRECMRELRSHGVDVSCLKFKKGRLGIYFFENGANQRPSNVI
                     YDRENSAISRITEEDIDWDASFKDMQWFHISGITPALSQNCADETIKAVRTAKDKGLT
                     VSFDLNYRAKLWNYGAEAYSVMSKIARYADILIGNEEDYQKSLGIKGPSAIGAQQLDL
                     DAYQAMCEIALKQYPNAKIAAVTLRESFSADNNDWSGMIVSEGRRFVSRKYHISDIVD
                     RVGAGDSFVAGLIYGLITKKDYQAALEYAAALSCLKHSISGDFALIEKGEIEKLLGGD
                     ASGRVQR"
     gene            complement(2898..3761)
                     /gene="eda"
                     /locus_tag="PLS88_05065"
     CDS             complement(2898..3761)
                     /gene="eda"
                     /locus_tag="PLS88_05065"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOU06745.1"
                     /translation="MVKKEEIGELFYRNGLIPVIKIDSADTADGLATALRRGGLSVAE
                     ITFRTKAAPKVIERFASCYEDILIGAGTVTKKDEVDAAISAGAQFIVSPGFNPSICEY
                     CLGKDVPIFPGVNNPSLIEQAMAMGLNILKFFPAEVSGGAKALNAFRSVYQQVSFIPT
                     GGIQENNINEYLSLKNVLACGGSWIVSADLLEAGKFDEIERLVKSARRAMIGFLPIVQ
                     EGIDEENQSRSMRDGGCIEVKTPSIKRTLAMLSYFKAYVIPGSEIENKQGLLEVSVGI
                     PGLHAQIHLVE"
     gene            complement(4051..4509)
                     /locus_tag="PLS88_05070"
     CDS             complement(4051..4509)
                     /locus_tag="PLS88_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOU06746.1"
                     /translation="MIVSMLSELSRYRGLSKNLDIAFDWLHKGLWRQLSTGKYSIEGD
                     AVFALIQEYTVKEHSECRFESHREYIDIQMMISGKEIIEVLPVNALKVIEPYKPDIEF
                     YALPTDRNANFLAMNAEQLAIFFPEDAHRPCMKAGALAESVRKVVVKVAI"
     gene            complement(4743..5504)
                     /gene="kduD"
                     /locus_tag="PLS88_05075"
     CDS             complement(4743..5504)
                     /gene="kduD"
                     /locus_tag="PLS88_05075"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HOU06747.1"
                     /translation="MILDKFKLDGKVAIVTGSSTGLGQGMSIALSEAGADVVGVDYVP
                     SKETASFIEAQGNKYLEIVANLLTIEPIQGILDKTISTFGRVDILVNNAGIIRREDAI
                     NFKEKDWDDVMDLNAKTVFFFSQAVARKYIEQGTGGKIINIASMLSFQGGIRVPSYTA
                     SKSAVMGLTRLLANEWAKYKINVNAIAPGYMATNNTAELRADRERSEEILDRIPAGRW
                     GTPEDVKGAAVFLASSASDYVNGYTVAVDGGWLAR"
     gene            complement(5533..6363)
                     /gene="kduI"
                     /locus_tag="PLS88_05080"
     CDS             complement(5533..6363)
                     /gene="kduI"
                     /locus_tag="PLS88_05080"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOU06748.1"
                     /translation="MEIREAINSEFAKKMGTEDLRKAFLVEKVFIVGDMTAIYSHVDR
                     MIIGGIVPTSVPIALPVSKELGTEFFLERREMGIINIGGEGSIEVDGNTYDVGQRDAL
                     YISMGSKYVIFSSKNPKEPAKFYFNSAPAHRACKTRLITFDQANHVALGSDAECNKRV
                     INQYIHPTVLETCQLVMGMTTFSEGSVWNTMPAHTHERRMEAYLYFDMPDDRVVFHLM
                     GKPQETRHIVVRNEQAIISPSWSIHSGVGTGKYTFIWGMAGENQTFDDMDNVNMTDLR
                     "
     gene            6557..7327
                     /locus_tag="PLS88_05085"
     CDS             6557..7327
                     /locus_tag="PLS88_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOU06749.1"
                     /translation="MADMKVQSLDRAFDILEILAREPIGLTLAEISAESDLPRSTTFR
                     LLAVLLQRDYVRKGSDNNCYRLGPGFIELSSNYLNSLELKTESAPFMRELASSLGTIV
                     FLARRQGNMMVYMDKEDQFTSLRKYAIIGEQRPIYCTSLGKALILDMDDEEIHNLLPG
                     NVLQKYGPNTHIDVESLIKDIHICKVRGWTHDNEEAEPGVNCVAAPIRDYRGQIIASI
                     STSWLLEIRPELEAEKVASFVVKAANGISKAMGFAERP"
     gene            complement(7428..7652)
                     /locus_tag="PLS88_05090"
     CDS             complement(7428..7652)
                     /locus_tag="PLS88_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOU06750.1"
                     /translation="MLFEQVRSAIEDVRPSLRADGGDIELVSVSDDGVVKVRLTGACG
                     ACPYSMMTLKQGIEAYLKKAVPQVKEVKQA"
     gene            7914..8669
                     /locus_tag="PLS88_05095"
     CDS             7914..8669
                     /locus_tag="PLS88_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOU06751.1"
                     /translation="MARIIVFVNQKGGVGKTTSAINIGTSLALQYKKTLLIDFDPQGN
                     LTSGIGGKLSHTNIYHVISGLAEIEQIIQPVPVPRLFLAPSSVDLSGATIELVDRPDR
                     NDYLRRAIAPVVDDYEYILIDCPPSLGVLTLNGLAAATEVIIPLQCEYFALEGLSLII
                     QTIGLVQKSINPSLRISGILLTMFDARTRLSQEVVRQVTEYFKDKVFKTIIPRNVRLS
                     EAPSHGLPAIMYDPTCIGAKSYEAVTKEIISRG"
     gene            8662..9573
                     /locus_tag="PLS88_05100"
     CDS             8662..9573
                     /locus_tag="PLS88_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOU06752.1"
                     /translation="MAKFGLGKGLGALIPEHQQFFEQSLEEQSASIVKLVSIEDLVPN
                     PDQPRKTFTQESLDDLAESIRRHGLLQPLLVHQEKSGKYIIIAGERRYRAAQIAGLEQ
                     LPVIVRNQDDENHRIELSLVENIQREDLDPIEEAQAYAKLMEITGATQEGVAEMVGKS
                     RVAVANTIRLLKLPEDMQVQLKQGIISPGHARALLSIEEETERRRLFEHIQLNNLSVR
                     QAEQEAQKIQGLLQGKRTKTRKQLDSLKTIEEIPLDPHLKELMEKLIERLGTKVEISG
                     SIDTGTIKIHYFSREDLERIYEVLKIE"
     gene            9794..10609
                     /locus_tag="PLS88_05105"
     CDS             9794..10609
                     /locus_tag="PLS88_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06753.1"
                     /translation="MPSTFNEALPKYISFQLGSPILRTSEHIGGELLLAPSLDGISII
                     TDNIKVNAKETNLIIEALVFLPNSSPADPFSISAQLDSLGLIFNQFSSLEGIQYWSAS
                     RKIMRTLYVESYRIDNPKSRNRISDPSTASKLKTLLSKPMYLHQKDQTFDAIIFEVHC
                     SITDSAFLMTNNNVTPVRMIGIPVLPENGLRTGFFAAPSPNGILLYFVTSIQSPSIAR
                     DRVFESASNKALALLHWFIEKASSQKLIDPVTLPWNFDDLPVDVRVSAIPNKK"
     gene            10755..12053
                     /gene="eno"
                     /locus_tag="PLS88_05110"
     CDS             10755..12053
                     /gene="eno"
                     /locus_tag="PLS88_05110"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOU06754.1"
                     /translation="MSIIEYIEAREILDSRGNPTIEVDVVLEDGTLGRAAVPSGASTG
                     EYEAVELRDGDKKRYLGKGVLTAVENVNTTIAGEICGLDSLEQIDIDRTLIELDGTEN
                     KGRLGANAILGVSMATARAAAEYLGLPLYKYLGGVHTNLLPVPMSNIINGGKHADNEI
                     DFQEFMIMPLGAESFREAVRMNAEVFHSLKSILKADGHNTAVGDEGGFAPNIGNEDAL
                     DYIMRAIEKTGYKPGEQIAIALDIAASELFEEGGGKGYRFWKSNPDRLFSSDEMIELY
                     MNWINKYPIVSIEDGLDQNDWEGYVRFTKTLGNKIQIVGDDFFVTNTKRLVKGIEMGA
                     CNSILIKVNQIGTVTETIEAINMAKRAGYTAIVSHRSGETEDTFIADLVVGLETGQIK
                     TGSMSRTDRIAKYNQLMRIEDEMEGIAEYWGHKVFYNIHS"
     gene            12294..13091
                     /locus_tag="PLS88_05115"
     CDS             12294..13091
                     /locus_tag="PLS88_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOU06755.1"
                     /translation="MLTRNLDQAREAYKRRDPEATRLAHSPSPPHQAEEKHTTAQGKY
                     LKSIIYGGLDGTITTFAAAAGIAGASLSPGIVLIVGLANLLADGLSMSIGDYLSSKSE
                     AEYQAAERAREKWEVENYPEGEKLELEEIYMDRGMSKEDAKAVVEIIAKDNKAWVDTM
                     MIEELGIIPSDESPFANAISTFISFALFGFIPIAGYVLALFIPSLDSIRFQLACVLTG
                     ITLFTLGALKTILTGKNWFKSGLEMLLVGGVAASAAYLVGLLLGGLA"
     gene            13124..13672
                     /locus_tag="PLS88_05120"
     CDS             13124..13672
                     /locus_tag="PLS88_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOU06756.1"
                     /translation="MKTACILLAEGFEEVEAITPADYLRRAGIDVTIAGITGKRVRGC
                     HGIIIETDSGQEALDKDYDAILIPGGMPGARNLAENALVRGLIIRHYKKGKIIAAICA
                     SPAVVLHAACNLLEGKKFTGYPGTEDQIKVGHFFPERVVVDGNIITSQGPGTAGEFAI
                     SIISLLEGPQKANEVAQHTLLK"
     gene            13843..14223
                     /locus_tag="PLS88_05125"
     CDS             13843..14223
                     /locus_tag="PLS88_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOU06757.1"
                     /translation="MDKECIQAKTAPAAIGPYTHAIRVGDFVFCSGQLGIDPSTGDLV
                     EGIEAQTNQVLRNLASVLEACGCTIANLVKTTIFLKDMSSFPKVNEIYAGYFQGDFPA
                     RSAIQVAALPKNALVEIEAIACRQ"
     gene            14235..15407
                     /locus_tag="PLS88_05130"
     CDS             14235..15407
                     /locus_tag="PLS88_05130"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HOU06758.1"
                     /translation="MYNFDTIVDRTNTYSIKWDRDELPEGCRDAISMWIADMDFACSP
                     DIVKAIQTRAEHPIYGYTKRSEKYYQSFIDWMRHRNGWSIKKDWILFSPGVVPALNLA
                     VLAYTHPGDKIIIQPPVYNPFSDAVLNNSRQLVENPLIIRDGRYTMNFEDLERKIDAQ
                     TKLLILCSPHNPVGRVWARDELSKLIDICSSRNVLIISDEIHSDIILGTEVHRCTASM
                     SDRAAAITITLTAPNKTFNLAGLQIANIVIPNSELRKAFMQQTTNIGLNLTNTFGMVA
                     QEVAYTKGEPWLEELLLYLKENYATLKSFIETHIPSLKVFPLEGTYLPWLDCRGLGLS
                     DSELHDLFLKKAKLWLDDGIMFGTGGSGFMRINIACPRSVLIKALEQLENSIMTNT"
     gene            15447..16082
                     /locus_tag="PLS88_05135"
     CDS             15447..16082
                     /locus_tag="PLS88_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOU06759.1"
                     /translation="MLTYVLVGFALAADAFAVSVSAAACANALPLLIGLRAAFMFGLF
                     QFLMPLAGWSLGSAFSALIQNYDHWIAFGLLAIVGSKMLYEALHARRKSACVDPENGT
                     TEKATTTAGIDHGLNSAANGIMKLNTLLVLSFATSIDAMAVGLSYNLLGMPIMLPSFI
                     IGTITFIVCIIGIEFGRRLRKLIQKGAEIVGGSILIIIGFRILIDHIIKGT"
     gene            16185..16859
                     /locus_tag="PLS88_05140"
     CDS             16185..16859
                     /locus_tag="PLS88_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOU06760.1"
                     /translation="MGASSNHKSSSQIQSYDEIKANEAYCLQNPGGIIFLCTRGSIPN
                     SQTSVPHSSLPQDSASLDFSPLPQTSVPRDYHNLPQRYDFAPLAWCIPYEYEPVSKLL
                     IVCDTSHKTFHDVKENSVFAIAFPSFEMRSLIQQVGSVSGFAVDKFDNFNVPYFYAKT
                     IDVRIPEGVVGWMECSLERIIIEGTSGILLGAVIKAFAISEGWKNRIHYLNDAIWYKP
                     GDLISE"
     gene            16939..18084
                     /locus_tag="PLS88_05145"
     CDS             16939..18084
                     /locus_tag="PLS88_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06761.1"
                     /translation="MKKKLFALSIVFLCTSLTMIFATPGISFSVNSIKTNGDIIAGIP
                     IPTGADIGLEIPLGSSLYSVTFRAAGGYESRMILRNPTTSVPIMAPSAIDNTNRFFWT
                     NAMAEIGVRRYLVREDDGGKNIWLFGLARGRYEDNATSFPTTVFADAQGMQSISAMLG
                     IAHDSTFWQEPNRKVGWYAEFSMEYSPTFANFTTAQMDFGRFNLTTEGFIPLTPEAGT
                     LRNPSMMAPYIVLYGVGDYAVGSHIPHEILTTYGGIKGTKGLGDMVRGAQPWGYEAPI
                     KGYASVELRFPDQSILENFALYPVGYVFGDIGAYGGLYDKPVGASFIDNSGVIASAGA
                     GVSVSVQNYLWLGAYAGWRWPITDPLASTYYSKPSGFFWGITFAAHY"
     gene            18168..19124
                     /locus_tag="PLS88_05150"
     CDS             18168..19124
                     /locus_tag="PLS88_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU06762.1"
                     /translation="MEIKKRYLSKCKTSFIIVVFVAILAFSIEGLCAQDQDPNSGFPI
                     IRIATWNVKDCSAINQKTKETFLLHSFIAEAIKGARIDIIAIQEIQTEGRNGADIAFL
                     QDALQDIGWPIPNVAWVRSPQNDDLAIFSRFPIEESRTILEPSGQPWPRPVLETCIDL
                     VDMRLYLYTAHFKAYDDQKSLSARIAQAQALASHLRQRFGQSIREAAIILAGDFNTIS
                     PYDMQSKTGTIELLRLRDNEDPTDDFLSLNLEWLYFKPTYKSKPYASITDHIIVSPLL
                     AAKADKNSIEIIDPPPTDKGFPLSDHKILVFNLDPSRLCLWQ"
BASE COUNT         5611 a         4413 c         3733 g         5504 t
ORIGIN      
        1 attgcgggga ctccttcctt ctatctttgg tttcctaatt gattccatta tacaaggttc
       61 gtccccgctt tgcattagaa aatacttcac ttagagagtt ttgaaagagc ctctctatat
      121 caaaacattc atcaaacctc gccacgatta atgttgtttc aattagcaaa ttattatcag
      181 actttatttc aactcataaa ttcgaatgaa gttttcatcg aataatttat agaaaaccat
      241 gacaatccat aatctggaat attatggaat tattgaatta caattgttac ctccgtccct
      301 ctattaagag cagccattct atattaaaga ttcccttttc tatatataaa caaatcgctc
      361 cctaattctc ttttgaaaga ttctattcat ttgggtaatt ttgggctata ctaattaaga
      421 tttctcttgt ttctcatccg caccaatttg ttgaattcaa atacggaagg ctttgcatga
      481 gttattcaga tccaacccat ctaggtatta ttgcttgccc aggagcagag gcgttcgcaa
      541 atcaggttat acgaaagctc tctggaatct ataagcatcg attcatgaga aaagctcaag
      601 ctattgcagg aagatatcat atcactcctg atctggttat tagaaagatc aactttgcaa
      661 atgactgtaa cactggcatg ctttttttgc caggagataa agaacgttat cgaccaccca
      721 aattcaagat cgatgtaaga catacttttt ttgcaaatgg cgagataaaa acagaaatcc
      781 ttgaatctat caggggaaga agtatttata tctttcagga tgtagaaaat catcaacctg
      841 tctcatttaa tgatggtact atccaaaaag tcctttctgt aaatgatcat atttttaacc
      901 tttttgtagc tattgacgct gccatgcaag ctggagctgc agaaataagt cttgtgctac
      961 caatatatcc ctattcgcga caacataaga aaaagggaag agagggatta actgcagctc
     1021 gcataggcaa gatgctggaa agccttggtg ttgatagaat catcactctt gatattcact
     1081 caaaggaaat tgagaatgca tttgaccggc tccgaatgga gaacttgcat gcttcttttc
     1141 agatcatcga aaagctgacg tcgattattg atatagcaaa agaagatata gtagtacttg
     1201 ccccagatac aggtgcagtc gatcgaaata aattctatgc aaatgcttta caaaagccct
     1261 tagctttact ttacaaagaa agagactatt caaaaatcac taaaagtgca gcagaaacca
     1321 atatcagtga aatgcgatta ctcggtaatg tctcgaataa agttgtcttt atggcagatg
     1381 acatgatcgg caccggcgga actcttatta aggcaatgag atatctaaaa gaacttggcg
     1441 ccagcaaagt tatatgtgca gttagccttc caattttttc agcaaatgca attgatgaat
     1501 ttgacgctgc ttacaaggaa ggattatttt atagagtaat aggaacaaat gccatatatc
     1561 ataatgaatt gcttaaaaaa gagtggtata tccagagtga cgtcactggc ctttttgcgc
     1621 aggtaattag cttgctgcat cacgatagat ccttgagctc tcttttggat aatcgtgatg
     1681 ttgtggctcg tcttatcaag aaaaagttag aaagtacagg tagtagtaga gtatcttcaa
     1741 atcagccaaa ggatgagaat ataagtgaag atgttattcc ttatggcgcc gctaataagc
     1801 catgaagacc catcctcgtc cttccaataa ttaaagtgaa ggcaaattaa aagcagtcta
     1861 acgttgtact ctaccagagg catctcctcc taagagcttc tcaatctctc ccttttcaat
     1921 aagagcaaaa tcgccagaaa tagaatgctt taagcaggaa agcgcagcag catattcaag
     1981 ggctgcctga tagtccttct tcgtaatcaa tccatatatt agaccggcga caaaactatc
     2041 tccagcccca actctatcca caatatcgga tatatgatat ttacgcgata caaacctccg
     2101 tccctcactg acgatcatcc ctgaccaatc attattatcg gctgagaaac tctctcgaag
     2161 agtcactgct gctatctttg cgtttgggta ttgctttaaa gcaatctcac acatcgcttg
     2221 atatgcatcg agatcaagct gttgagctcc aatagctgaa gggcccttaa tacctaatga
     2281 tttttgatag tcttcttcat tccctataag aatatctgca tatcgcgcaa tttttgacat
     2341 cacactatat gcttccgctc catagttcca taatttggct ctataattta aatcaaaaga
     2401 tacagtcaat cccttgtctt tagcagtacg aacagcttta atcgtttcat cagcgcaatt
     2461 ttgagaaaga gcaggtgtaa ttcctgatat atgaaaccat tgcatatctt taaatgatgc
     2521 atcccagtcg atgtcctctt cagtaattct tgaaattgct gagttttccc tatcataaat
     2581 gacattactt ggacgctgat tggctccatt ctcgaaaaaa tatattccca gcctcccctt
     2641 cttaaatttt aggcatgaaa catcaacacc atgcgatcga agctctctca tgcattctct
     2701 tccaattgga ttgtctggca aaatactaat aaagcgcgct ggaatgccca atcttgagag
     2761 agcaacagcc acattcgctt cacttccgcc aaaagttgct tcaaaaaaag gcgactgtag
     2821 taatctttcc tgtccaggag attttaacct aagcataatc tccccaaatg ttgctatgct
     2881 cattccaatt ctcctcccta ttcaacaaga tgaatttgag catgtaatcc tggaataccc
     2941 acgctcacct caagtaaacc ctgtttattt tcgatctcgg aaccaggaat aacataagct
     3001 ttaaaataag aaagcatagc caatgttctt ttgatcgatg gggttttgac ttcaatacaa
     3061 cctccgtccc tcattgatct gctctggttc tcctcatcta tgccctcctg cacaattggc
     3121 aggaatccta tcatcgctcg tcgtgcagat tttacaagtc tctcaatttc atcaaatttt
     3181 cctgcttcaa gaaggtctgc tgagacaatc catgagccac cgcaagccaa cacattcttc
     3241 aaagaaaggt attcattaat gttgttttcc tgaattcctc ctgtagggat gaatgacacc
     3301 tgctgataca ctgacctaaa tgcattaaga gccttagcgc caccagagac ctctgcagga
     3361 aaaaatttca aaatgttaag gcccatagcc atagcttgct ctataagcga agggttgttt
     3421 acccctggga aaataggaac atctttcccc aaacagtatt cacatattga tgggttaaaa
     3481 cctggagaga caataaattg tgctcctgcc gaaatagctg catctacttc atcttttttc
     3541 gtcaccgttc cagcgccaat caaaatatcc tcatagcatg atgcaaatct ctcaattact
     3601 ttaggtgcag cctttgtcct aaaagtaatc tcagcaacag acaacccacc tctacgaagt
     3661 gctgtagcaa gaccatcagc tgtatccgca gaatcaatct ttattacagg gataagacca
     3721 tttctataaa atagttctcc tatttcctct ttttttacca ttgcataatc cttaaaaatc
     3781 tatgttatgt tctagtccac atagctaaag tcaagtctct cctttagcta gagccgacaa
     3841 aatccacgtt aacatcgtat tttttcagtc ctaattgttt cttttggaaa atcctccgtc
     3901 tcctattttc tccgatatcc aatacacaat ttatctgatt tccttagtta gcaatttcca
     3961 atcaaaattc cagccagata aaatctttcc gatccgttct tcaattcctg attgaaataa
     4021 atatgatatc cattctttta actttaatac ttatatagct acttttacca caacttttct
     4081 caccgattcc gccaatgctc ccgctttcat acatggccga tgggcatcct cggggaaaaa
     4141 aattgcaagt tgctcagcat tcatcgccaa aaaattagca tttctatctg ttggtaaagc
     4201 ataaaattct atatcaggct tgtaaggctc aattactttg agtgcattta ctggcagcac
     4261 ctcaatgatt tcttttccag aaatcatcat ttgaatgtct atatattctc gatgagattc
     4321 aaatctgcac tctgaatgtt ctttaactgt atattcttga atgagcgcaa agacagcatc
     4381 gccctcaata gaatacttcc ctgttgaaag ctgacgccaa agtcccttat ggagccaatc
     4441 aaaagcgata tctagatttt tactaagtcc tcgatatcta gataattctg aaagcatgct
     4501 gacaatcatt ttcttactcc ttatataggg aattctgaat aaataaacaa tcgcttttgt
     4561 taattattca ataaacctcc gtccctagag ctatgcagag ccataggacc ataacccttc
     4621 tgtcttctaa gtaaaaacta cctatagtgc ccatatgtca gactatttaa atctgcaacc
     4681 ctcacatcta ggctgtatta atcctccgtc cctctaatcc ccacatgaac ttgttattac
     4741 tgttatctag ccagccaacc gccatcgaca gcgacagtat acccattaac ataatcgctt
     4801 gctgatgatg ctaaaaagac agcagcgcct ttcacatctt caggcgtacc ccatcgaccc
     4861 gctggtatgc gatccaggat ctcctctgac cgctctctat ctgctctcag ctccgctgta
     4921 ttgttagttg ccatatagcc tggagcaatt gcattcacat tgatcttata ttttgcccat
     4981 tcatttgcta ataatcttgt cagacccatc acggcacttt tcgatgctgt ataagaaggc
     5041 acccttattc ctccctggaa tgaaagcatt gatgcaatat tgatgatctt tcctcccgtc
     5101 ccttgttcga tatattttcg cgccactgcc tgactaaaga agaatactgt cttggcattc
     5161 aggtccatga catcgtccca atctttctcc ttaaaattaa ttgcatcttc tcgcctaata
     5221 attccagcat tgttgaccaa aatatccact cgtccaaaag tgcttatggt cttatctaag
     5281 atgccctgaa ttggctctat tgtcagaagg tttgctacaa tctctaggta tttgttccct
     5341 tgtgcctcta tgaaactagc tgtctccttt gatggtacgt aatctacccc tacaacgtct
     5401 gctccagctt ctgaaagtgc aatgctcatt ccttgtccga ggccagtcga actcccggtt
     5461 acaatcgcta ctttgccatc gagcttaaat ttatccaaaa tcatatcttt tctcctttca
     5521 atatagatca gttcaacgta gatcagtcat attgacatta tccatatcat caaaagtctg
     5581 gttttccccg gccatgcccc aaataaaggt atatttccca gttcctactc ctgaatgaat
     5641 cgaccagctc ggactgatta tggcctgctc gtttcgtact acaatatggc gcgtttcctg
     5701 cggtttgccc atcaaatgaa atactactct gtcatctggc atatcaaaat atagatatgc
     5761 ctccattcgc ctctcatgtg tgtgtgccgg cattgtgttc cagacactgc cttccgaaaa
     5821 agtcgtcatt cccattacaa gctggcatgt ttcgagaact gtcggatgaa tatattgatt
     5881 gatcactctt ttattgcact ccgcatcact acccagcgcc acatggttag cttgatcaaa
     5941 agttataagc cttgttttgc atgctctatg agcgggtgcg ctattgaaat agaattttgc
     6001 cggctctttt ggattcttgc ttgaaaatat tacatatttt gaacccatcg agatatataa
     6061 agcatctcgt tgtccaacat cataggtgtt gccatctacc tcgatagatc cttctcctcc
     6121 gatattgata attcccatct ctcgccgttc tagaaaaaac tctgtcccca attctttgct
     6181 cactggtagc gcgataggga cggaggttgg aacaatacct ccaattatca tcctatctac
     6241 atgactatag atggctgtca tgtctccaac aataaagact ttctccacca aaaaagcctt
     6301 gcgcaaatcc tctgtcccca ttttttttgc aaactcagaa ttaatcgctt ctcgaatttc
     6361 catctttgct ccttgtatga taaattattg gtacatgatt tttaggtttt gatgccaata
     6421 tttggcattg tgcctaaaga taattagtaa aaacgattcc acaatatgga accctattcc
     6481 atataatagc ttgcttttct atcttgtcaa tactaaaata aatctttatg gttaaaatag
     6541 gaataaagga ggccctatgg ccgatatgaa agtacagtct ctcgatcgcg ccttcgatat
     6601 actcgaaatc cttgcgcgcg agccaattgg tcttaccctt gcagagattt ctgcagagtc
     6661 ggatcttcct cgcagtacaa cttttcgttt gctcgccgtt cttcttcagc gcgattatgt
     6721 gcgaaaaggc tcagataata actgttatcg attggggcca ggttttatcg agctctctag
     6781 caactatctg aatagtctag aactcaagac ggaatctgct cctttcatgc gagaactcgc
     6841 atcctcattg ggaacgatcg tatttcttgc acgtcgtcag gggaatatga tggtttatat
     6901 ggacaaagaa gatcaattca ccagtcttag gaaatatgca atcataggag aacaaaggcc
     6961 tatctattgc acatctctag ggaaggctct catattagac atggatgatg aagaaattca
     7021 caatcttctt cctggtaatg tattgcaaaa atatggtcca aacacacata ttgatgtaga
     7081 atctctcata aaagatattc atatctgtaa agttagagga tggactcacg acaatgagga
     7141 agcagagccc ggagtaaact gtgtcgcagc tcctatccgt gattatcgcg gtcaaatcat
     7201 agcctccatc agcacatcct ggcttttgga aattcgaccc gaattggagg cggaaaaagt
     7261 agcttcattc gtagtcaaag cagctaatgg aatatcaaaa gcaatgggat ttgctgaaag
     7321 accttaaaaa ttgcaataag cctggcttaa aaaatagatt taatctagac aagaaataga
     7381 caattagctt gcaaggcatt cccttacatc aggcgagaat tatcttctta ggcttgtttt
     7441 acttctttta cctgtggtac tgctttcttg aggtacgcct caatgccttg cttaagggtc
     7501 atcatggaat aaggacatgc accgcacgct cctgtgagtc ttactttaac cacaccatcg
     7561 tcgcttacag atacaagttc tatatctcct ccatccgctc tgagggacgg ccgtacatct
     7621 tcaatcgcag atctaacctg ttcgaacagc attcttatat cctctactca aattaaatta
     7681 ccaaatttgc ttcaaaaaag tcaaggaggc tggctagatc cttgctggct tagcctttta
     7741 tcctgctata aacaataaac aaagtcgaat aatcgaagat taagatacaa aatgatccag
     7801 acactatttc tgatatgtat gtaattgtat caactgcctt tttctgcgct aaacctgtat
     7861 agctgaagct cgctctatat gttaaaatca tcatagcata tcgggaaggt aaaatggctc
     7921 gtattatcgt atttgtcaac caaaagggcg gtgtagggaa aaccacgagc gctatcaata
     7981 ttggtacatc tctcgccctg caatataaaa aaaccctcct tatagacttc gatccccagg
     8041 gaaatctcac ctctggaatt ggaggcaagt tatctcacac caatatttat catgtgattt
     8101 caggccttgc ggagatagag caaatcattc agccggtccc tgttccgcgc ctttttttgg
     8161 ccccatcctc agttgacctc agtggtgcaa ccatcgagct cgtcgatcgt cctgacagga
     8221 atgactatct gagacgtgcg atagcccctg ttgtggatga ctatgaatat atccttatag
     8281 actgtcctcc gtcccttgga gtgcttacat tgaatggact ggcagccgct acagaagtca
     8341 taatacctct tcaatgcgaa tacttcgctc tagaagggct ttcgcttatc atccagacaa
     8401 tcggacttgt acaaaagagt ataaatcctt ctttgagaat tagtggtatc cttctcacca
     8461 tgttcgatgc tcgcacgcgg ctttctcaag aggttgtcag gcaagtaaca gaatatttca
     8521 aggacaaagt tttcaagaca atcattccgc gaaatgtccg tctctcagaa gctccatctc
     8581 atgggctacc tgccattatg tatgatccca cctgcattgg ggcaaagagc tatgaggctg
     8641 tcacaaagga gataataagc cgtggctaaa ttcggtcttg gaaaagggct tggagccttg
     8701 attcccgaac accaacaatt ttttgaacaa tctcttgaag aacaatcagc atctattgta
     8761 aagcttgtat caatagaaga cctggttccc aatccagatc aaccaagaaa gacctttact
     8821 caggaatcat tagatgacct tgctgaatct attcggcgtc atggacttct gcagccactt
     8881 ctcgttcatc aagaaaaatc gggcaaatat atcataattg ctggtgaacg aagatatcgc
     8941 gctgcgcaga tagcagggtt ggagcaacta cccgtcatcg tacggaacca ggatgacgaa
     9001 aaccatcgca tagaactatc tttagtagaa aatatccagc gcgaagacct tgaccctata
     9061 gaagaagctc aagcttacgc aaagctgatg gaaataaccg gtgccactca agaaggcgtc
     9121 gctgaaatgg taggcaaaag ccgcgtcgct gttgcaaata ctattcgtct tctaaagctt
     9181 cctgaagaca tgcaagtcca gctcaagcag ggtattatca gccctggaca cgccagggct
     9241 cttctttcga tagaagagga aacagaacgt agaaggctct ttgaacatat tcaactgaat
     9301 aatctatcag ttcgtcaagc agaacaggag gctcaaaaaa tccagggctt gctacaaggc
     9361 aaaaggacaa aaactagaaa gcaacttgac tctcttaaga caatagaaga aatccctcta
     9421 gaccctcact taaaagagct aatggaaaag ctcatcgaaa gattaggtac aaaagtcgag
     9481 atatcaggca gcattgatac tggtaccata aaaatccact acttctctcg agaagacctt
     9541 gagcggatct atgaagttct taagattgaa taaaattttc ttggacaatc gatgttttcg
     9601 ctgccaattg aaagccaatg gagacaatga aaactctggg ggacggaggt tgcaaacttt
     9661 ccaagccctc ctccgcttgt cttctttgat ccccattttt ctgttgatct ttcttgtgga
     9721 tctttcagcc caacaatcgc tacaatcatc aggctcacaa ggtgcaagga acatctctaa
     9781 aataagtgcc cctttgccat caacatttaa tgaagcttta cctaaataca tcagtttcca
     9841 actaggttct cctatccttc gcacatctga acatattgga ggcgagcttt tgctagctcc
     9901 gtccctcgat ggtatctcga ttatcacaga caacataaaa gttaatgcaa aggagaccaa
     9961 tcttataatt gaagctctag tcttccttcc taacagctca cctgcggatc cattttcaat
    10021 ctctgctcag cttgattcac tagggctaat attcaaccag ttttcttcgt tagaaggcat
    10081 tcagtattgg tcagcaagca gaaaaataat gcgcactctc tatgtggaat cgtaccgcat
    10141 cgacaaccca aagagcagaa acagaatcag cgatccatca acagcatcta aattgaaaac
    10201 tcttctttca aaacctatgt acctccacca gaaagatcag acattcgatg caattatatt
    10261 tgaagtccat tgttcaatta cagattcagc atttcttatg accaacaaca atgttacccc
    10321 tgtacgtatg attggcattc ctgtcttgcc agaaaatggg cttcgcacag gattttttgc
    10381 agctccgtcc cccaacggta ttctcctcta ttttgttacc tcaatccaat cgccctcgat
    10441 tgcacgagat cgagtatttg aatcagcctc gaataaagca cttgcacttc tgcattggtt
    10501 cattgagaaa gcatcctccc aaaaacttat cgatcccgtt accctccctt ggaatttcga
    10561 tgacttgcct gtagatgttc gagtatcagc gatacccaac aagaaataat catccattct
    10621 agttaaatcc atttgccttc acaacaaaaa tccatacttg aaatcctgaa aaaccgagaa
    10681 aaccttccaa gtcttgacga attataatat tatcgatatc gttcagcaca tgtctattca
    10741 aaaggagaat ctctatgagc attatagaat atatcgaagc tcgtgagata cttgactcac
    10801 gtggaaaccc gactatcgaa gtggatgtag tgctcgagga cggtacacta ggtcgtgctg
    10861 cagtaccatc cggtgcctca actggcgaat atgaagcagt ggaacttcgc gatggcgata
    10921 agaaacgata tcttggcaaa ggcgttctta ccgcagtaga aaatgtaaac acaaccatag
    10981 caggagaaat ctgcgggctc gattctcttg aacaaatcga catcgatcga acgctgattg
    11041 agctcgatgg aactgaaaac aaaggcagac tcggcgcaaa tgctattctt ggcgtatcca
    11101 tggcaacggc tcgtgctgct gccgaatacc ttggccttcc cctttataaa taccttggtg
    11161 gggtgcacac caacctttta cctgtcccaa tgtccaatat catcaacggc ggaaaacacg
    11221 cggataacga gatagatttc caggaattca tgatcatgcc tctcggtgca gaatcattcc
    11281 gcgaagcagt ccgcatgaac gccgaagtct tccattcact aaaatccata ctgaaagccg
    11341 atggtcacaa cacagccgtt ggtgatgaag gtggattcgc tccaaacatc ggcaatgaag
    11401 atgctctcga ttacatcatg cgggccattg aaaaaactgg ctacaagcca ggtgaacaga
    11461 tagctatcgc tcttgatatt gcagcaagtg aactattcga agaaggtggc ggcaaaggct
    11521 accgcttctg gaaatccaac cctgatcgac ttttctcctc cgatgaaatg atcgagctct
    11581 atatgaactg gatcaataag taccctattg tatctatcga agatggcctg gatcagaacg
    11641 actgggaagg ctatgtcaga tttacaaaaa ctctcggcaa taaaatccag attgtagggg
    11701 acgatttctt tgtcaccaat accaagcgcc ttgtaaaagg catcgaaatg ggcgcttgca
    11761 attctattct tatcaaagtc aatcaaatcg gaactgtcac cgaaaccatc gaagctatta
    11821 atatggcaaa gcgagctggc tacaccgcca ttgtctccca tcgttcaggc gaaaccgaag
    11881 acactttcat tgccgaccta gtcgttggtc tagaaacagg ccagataaaa acaggttcta
    11941 tgtcgcgtac tgaccgcatc gcaaagtaca accaactaat gcggatcgaa gacgaaatgg
    12001 aaggaatcgc tgagtattgg ggtcataagg tcttctataa catccatagt tgagaattac
    12061 caaagggctc tttagaaact ttttaaaaga gctgtctata aaaagatcta cttaaggttt
    12121 cctctgtctc tgtgccgctc gatggtattt tgtgctccaa ggttacctat acctaaacct
    12181 ccgtccctcg tgcttacctc attgttgctt ttagactaaa ataaataatc atcaaagtcg
    12241 ctagagttca tcatagcgcc tttttcaata agaaatgttt tataggagaa tctatgctca
    12301 cgcgaaatct tgaccaagcg agagaagcgt acaaacgcag ggatccagaa gctacacgtc
    12361 tcgcacacag tccctctcct ccgcaccaag ccgaagaaaa acacacaacg gctcaaggca
    12421 aatatctaaa aagcataata tatggcggcc tcgatgggac tatcacaacc ttcgccgctg
    12481 ctgcgggtat tgcaggtgct tctctgtccc ctggaatagt actcatagta ggacttgcta
    12541 acctgctcgc agatggcctg tcgatgtcga ttggcgatta cctttcatcg aagagtgagg
    12601 cggaatacca agccgctgag cgagcacgcg agaagtggga agtcgagaat tatcctgaag
    12661 gagaaaaact agaactcgaa gaaatctata tggacagagg catgtcgaag gaagatgcaa
    12721 aagccgtagt ggaaattatt gctaaagata acaaagcatg ggtcgataca atgatgatcg
    12781 aggagcttgg aattatcccc tcggatgaat cgccttttgc aaacgctatc tcaaccttca
    12841 tttcttttgc cctgtttgga ttcattccta ttgcaggcta tgttttggcc ctttttatac
    12901 cttcgctcga ttcgattcgt tttcagctcg cttgtgtatt gactggaata accttgttca
    12961 cgctcggagc actaaagaca attctgactg ggaaaaactg gttcaaaagc ggccttgaaa
    13021 tgctgctggt aggaggtgtt gcggcttctg ctgcctatct cgttggtttg ttattaggcg
    13081 gattggctta atatattgcg cattaattaa ttaggaggaa gcaatgaaaa cagcttgcat
    13141 acttcttgct gaaggattcg aagaagtcga agccataaca ccagccgatt atttgcgccg
    13201 cgcaggcatc gatgtaacta tagctggaat taccggaaag agggtcagag gttgccacgg
    13261 tatcattata gaaactgact caggtcaaga agcgctagat aaagactatg atgcaatcct
    13321 gattcccggt ggcatgcccg gcgctcgcaa tcttgctgaa aatgctttgg ttcgcggtct
    13381 gatcattcgg cattataaaa agggcaagat tatcgcagcc atctgtgctt ctccagccgt
    13441 tgtgctccat gctgcctgca acctactgga ggggaaaaaa ttcacaggct atccggggac
    13501 ggaggatcaa attaaagtag gtcatttctt tcctgaacgg gttgtcgtag atggcaacat
    13561 aatcacttcg caaggtcctg gtaccgccgg cgaatttgcg atttctatta tttcattact
    13621 agaaggaccc caaaaagcga atgaagtcgc tcagcatacg ctcctaaaat gattttatgt
    13681 ttcggcagga ttatatacac ttgtcatgtc tcttgttgag aaaataataa ctatacagaa
    13741 aggaatgttg tatatgcttt ctgcatcgtg ctatttatat aaagaatcat gaattactag
    13801 gagttcatcc cggtccatct aatatattct aaaggagtct ttatggataa ggaatgcatt
    13861 caagcaaaga cagcgccagc tgccattggc ccctatactc atgctattcg cgttggtgac
    13921 tttgttttct gttctggcca gctaggcata gatcccagca ccggtgatct tgtcgaaggt
    13981 atcgaagcac aaacaaatca agtgcttaga aaccttgcct ctgtactcga ggcatgcggt
    14041 tgtacgatag ccaatcttgt aaaaaccacc atttttctca aagacatgag tagctttccc
    14101 aaggtcaacg aaatatatgc aggttatttt caaggtgatt ttccagcacg cagtgcaata
    14161 caagtcgctg cccttcctaa aaatgctcta gttgaaattg aagctatagc ttgtaggcaa
    14221 taaggagtct cggaatgtat aactttgata caatcgtaga tcgcaccaat acttatagca
    14281 tcaagtggga tagagatgag cttccagaag gctgcagaga tgcaatatct atgtggattg
    14341 ctgacatgga ttttgcttgt tctcctgata ttgtcaaagc aatccagacg cgtgcagaac
    14401 atccaatata tggttataca aaacgctcag aaaaatatta tcagtccttt attgactgga
    14461 tgaggcatcg caatggctgg agtatcaaaa aagactggat actattttcg ccaggcgtcg
    14521 taccagcact caatctagct gttctagctt atacccatcc tggtgataaa attatcatac
    14581 agccgccagt ctataatcct ttttctgatg ccgtgcttaa caattctcgt caactagtag
    14641 aaaatccgct gatcattaga gatggacgct ataccatgaa ttttgaagat ctcgagcgta
    14701 agatagatgc tcaaacaaaa cttctcattc tctgtagtcc acataatccc gtaggtcgag
    14761 tttgggccag agatgagctt tctaagctga tcgatatctg ttcttctcga aatgttttaa
    14821 taatctctga tgaaatacac tctgacatta ttctggggac ggaggttcat cgttgcacag
    14881 ccagcatgtc agacagagca gcggctatta ctatcactct taccgcgcca aacaaaacct
    14941 tcaatcttgc tggcttgcag attgctaata ttgttatccc gaatagcgaa cttcgtaagg
    15001 cctttatgca gcaaaccaca aatattggcc ttaacctaac taataccttc ggcatggtcg
    15061 cgcaggaagt tgcgtacaca aaaggagaac cctggcttga agaactcctt ttatatctca
    15121 aggagaacta cgctactcta aagagtttta tcgaaacaca cattccatct ctcaaagtct
    15181 tcccgctaga aggaacttat ttgccatggc tcgactgtcg cggattagga ctcagcgact
    15241 ccgagctcca tgatctattc ctaaaaaagg caaaactctg gctcgatgat ggtattatgt
    15301 ttggcacagg tggttcagga tttatgcgca tcaatatagc ttgtccaaga agcgtgctca
    15361 taaaagccct tgaacagtta gaaaattcaa taatgacaaa tacatagcat aaagtacccc
    15421 tttgctataa aataaaaccc ctctttatgc tcacatatgt gttggttggt tttgctctag
    15481 cagccgatgc gtttgcagtc tctgtttctg cagcggcttg cgctaatgct ttgcctcttt
    15541 tgattggcct tcgtgcggca ttcatgttcg gcctctttca attcttgatg ccactcgctg
    15601 gctggtcgct gggttcggca ttcagcgctc ttatccaaaa ttacgatcac tggatagcct
    15661 ttggactact agccatagta ggcagcaaaa tgctctatga ggctcttcat gcacgaagaa
    15721 aatcagcctg tgttgatcca gaaaatggca ctacagaaaa agccacaaca actgctggca
    15781 tagatcatgg cttaaattct gcggcaaatg gaattatgaa gctaaataca ttgttggtgc
    15841 tttcttttgc tacaagcata gatgcaatgg ctgtgggact atcctacaat cttcttggta
    15901 tgccaatcat gctaccaagc ttcataattg gtacgattac ctttatcgta tgcataatcg
    15961 gaatcgaatt tggcaggcgg cttagaaagc taattcaaaa aggcgcagaa atagtcggag
    16021 gcagcattct catcattatc ggattcagaa tactaattga ccacatcata aaagggactt
    16081 aatgtaataa aaaggttaaa tcttatagaa ggcttttcta aagcagactt ataagagaac
    16141 ctttttacat attttttacg aacccatctc tagaggaggt ttctatgggg gcttcttcaa
    16201 atcataagtc ttcttcacaa attcagtctt acgatgagat taaagcaaat gaagcctatt
    16261 gccttcaaaa tccaggcggc attatcttcc tttgcacacg cggctctatt cccaattctc
    16321 aaacctccgt ccctcatagc tccctccctc aagattccgc ctctcttgac tttagcccac
    16381 ttcctcaaac ctccgtccct cgagattacc acaatctacc tcagcgctat gattttgcac
    16441 cactcgcttg gtgcatcccc tacgaatacg agccggtgag caagctgctt atcgtctgcg
    16501 acacttcgca caagaccttc cacgacgtca aggagaatag cgtatttgcg atcgccttcc
    16561 cctcattcga aatgcgatct cttattcagc aagttggctc ggtgagcgga ttcgctgtcg
    16621 ataaattcga taattttaat gtaccttatt tttatgcaaa gacgatcgat gttcgcattc
    16681 ccgaaggagt tgtgggctgg atggaatgct ctcttgagcg aattataata gaaggaacat
    16741 caggtatttt gctgggtgcc gtcatcaaag cgttcgccat ttctgaaggc tggaaaaacc
    16801 ggatacatta tctgaacgat gcaatctggt ataaaccagg cgacttgatc tctgaataat
    16861 agctggagag tcgaatatag aataagaatt agaagcaaag accaaataat ggtcaaagaa
    16921 taaggaggtt tttgacttat gaaaaagaaa ctgttcgctt tatcgatcgt attcttatgc
    16981 acttctctga ccatgatatt cgcaacacca ggtattagct tttctgtgaa tagcatcaag
    17041 acaaatggcg acataatcgc aggaataccc atacctacgg gagcagacat tggccttgaa
    17101 atccctcttg gttctagttt gtattcggtt accttccgag cagcgggtgg ttacgaatcg
    17161 agaatgatcc tgcgcaatcc tacaacctcc gtccccatca tggccccatc agccatcgac
    17221 aacacaaata gatttttttg gaccaatgca atggcagaaa ttggcgtccg gcgttatttg
    17281 gttcgtgagg atgatggcgg aaaaaatata tggcttttcg gactcgctcg aggtcgatac
    17341 gaagacaatg ccacaagctt tccaaccaca gtatttgctg atgctcaagg catgcaatcg
    17401 atctccgcaa tgttgggcat tgctcatgat tcgacttttt ggcaagaacc caatcgaaag
    17461 gttggctggt atgcagaatt ttccatggaa tatagcccga catttgccaa cttcaccaca
    17521 gcgcagatgg attttggccg attcaatctg actacagaag gctttatccc actcacgcca
    17581 gaagcgggaa cgctgcgaaa tccttctatg atggccccat atattgtgct ctatggcgta
    17641 ggggattatg cagttggttc gcatattccc catgagattc tgacaactta cggagggatc
    17701 aagggaacca aagggcttgg cgacatggtt cgaggagctc agccttgggg ctatgaagct
    17761 ccgataaaag gctatgcaag tgtggaattg cgttttcctg accaatcgat cctagagaat
    17821 tttgcgctgt atcctgttgg atatgtattt ggagacatag gcgcttatgg tgggctctat
    17881 gacaagcccg tgggcgcgag ctttatagat aattcgggag taattgcatc ggcaggtgcc
    17941 ggggtctctg tatcggttca gaattatcta tggctcggcg cttatgctgg ctggcgctgg
    18001 ccaattaccg atccgctagc atcgacctat tactcgaaac ctagcggctt tttttgggga
    18061 attacttttg cagcgcatta ctgaggtaag ccctagcgcc tggaaatcct agaccaggaa
    18121 aagtaaataa tcttgcttct cagcatctaa attgatgtgg gtttgtaatg gagattaaaa
    18181 agcgctatct gtcgaaatgt aaaacttctt tcatcatagt agtcttcgtt gcgatactcg
    18241 ctttttccat tgagggtctt tgtgcacaag atcaagatcc taactccggt ttccctataa
    18301 ttcgcattgc gacatggaat gttaaagact gcagcgccat caatcaaaaa acaaaagaaa
    18361 catttctact gcactctttt attgccgagg ctataaaagg agcaagaatc gatatcattg
    18421 ctattcaaga aatacaaacc gaaggacgca acggcgcaga tatcgcattc ctgcaagatg
    18481 cattgcaaga catcggctgg cctataccaa atgttgcatg ggttagaagc ccacagaatg
    18541 atgatcttgc aatcttctct cgatttccta tagaagaatc acgaactatt ctcgagcctt
    18601 ccggacaacc ttggcctcgc cctgtcctgg aaacatgcat cgatttagta gatatgcgcc
    18661 tttatctata taccgctcac tttaaggctt atgatgatca aaaatcgctt tctgcgcgca
    18721 tagcccaagc tcaagctcta gcttcgcatc tcagacagcg ctttggacaa tcgataagag
    18781 aagcagcgat catcctagcc ggcgatttca acaccatctc accttatgac atgcagtcca
    18841 aaacaggtac tatcgaactc cttcggttgc gagataatga agatccaaca gatgattttc
    18901 tgagcctcaa ccttgagtgg ctttacttca agccaaccta taaatcgaaa ccttacgcaa
    18961 gcataacaga ccacattatt gtttcgccgc ttcttgcagc aaaggccgat aaaaattcaa
    19021 tcgaaattat cgatccacct ccgaccgata aaggttttcc attatcagac cacaaaatac
    19081 ttgtgtttaa tcttgatcct agtaggctgt gcctatggca ataaacagga ttgtcgatac
    19141 tcaatttatt ctgtcgcgat aagttagcta atatttaaat gaaattataa aaaagcgccc
    19201 aaaaggcgca tcaataaccg aaaggctcta taagaaaaat tgccggcagt gggacttgaa
    19261 c
//
LOCUS       DAOSOB010000035        18663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_2985_length_18663_cov_65.322979, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000035 DAOSOB010000000
VERSION     DAOSOB010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 18663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18663
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2985_length_18663_cov_65.322979"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1670)
                     /locus_tag="PLS88_05155"
     CDS             complement(222..1670)
                     /locus_tag="PLS88_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU06763.1"
                     /translation="MFRNQDSLNLPSAWRPSLERAAYNILIFDHLVYVRFLCIEKNKI
                     LTRRAMMSEKKGFKPYLGITFLIGFGFFTMGLMDPLYDTYVPIFLSRYLSKMSLVGFF
                     MTLDNILAIFLIPLVSAWSDHTYTKLGRRMPYILVLLPLTALLFGAIPYAAEASLVAL
                     LVTLILLNVTKQSVRGPVVALMPDTIPADYRSEANGVINTMGGIASIVGTIGLARLMD
                     LDTTLPILGSTKDRLPFPLGGMFVILAVILLLIFIREKQPDPSEVQEQKVPILESFRN
                     VAAQKDKSALYILIAIFLWFLGYQGVLPFIGKYSVDVLKTSSGTAALAAGMVGIAYAI
                     FAIPSGYVAHRFGRKKTIRACLLVISILTLLLFAHKWITASLPNSLRLASFWAIMFIF
                     GIFWVSIITNSFPMLWQMAEWGTIGIYTGLYYTASQAAAILGPVLTGAIIDVFGYQGI
                     FLFCTLCMLCARAIMGKVTKGEPSEIQNPAQK"
     gene            complement(1552..2808)
                     /locus_tag="PLS88_05160"
     CDS             complement(1552..2808)
                     /locus_tag="PLS88_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOU06764.1"
                     /translation="MVQQKIPCIPLASFQNRETENHKTRYESLVSRLPPLESSCRQEL
                     HYFSSPGRTELGGNHTDHNNGCVLAASVDLDMLAVASPRSDYLISLASEGYSPIRIDL
                     RDTSFKIEERGSPASIIRGLAAWLTHKGMQIGHGFDIAMDSRVPAGSGLSSSAAFELL
                     IAAIFDDMGKYEMSPIDWAIAGQWAENEYFGKPCGLMDQIACAEGGIVFIDLYEPGKP
                     KIEKLTYDFASNGLILAIVNTGSNHEDLTIEYSDIPKEMKCVADLFGRPAMRGIEKQD
                     LLAKLSDIRIRCGDRALLRAWHFVHENSRPVKMVEALNRNDISAYLSIVNDSGRSSWH
                     YLQNIHTGNPHQQSLSIALMLSEDLLSPEGAWRVHGGGFAGSIQAYVPESRFPEFTER
                     METVFGEGCVQHINIRPFGVCKVLMH"
     gene            3127..4419
                     /locus_tag="PLS88_05165"
     CDS             3127..4419
                     /locus_tag="PLS88_05165"
                     /inference="COORDINATES: protein motif:HMM:NF006388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU06765.1"
                     /translation="MNQLAEELNAVLAGTVAERLFSEMGRRMYFPKGIITQSAEAGEK
                     AYRFNATIGMAYEHGQPMMLDALRAELPGLTPTEAVAYASTGGIPELRKTWKESLYRK
                     NPSLNGKKISLPTVVPGLTAAISYICDMFVEPGDTVILPDLHWPNYRLIVEERKSAAG
                     ITYPLFAGKGYNIEGLLEGVRQASQRRGKAIIILNFPNNPTGYSLTLDEADRVTAGLL
                     EIAQQGTDILVIADDAYFGLQYEDDLLCESIFARLADLHPNILAIKADGPTKEDYVWG
                     FRIGFVTFGAAGFGDEHYEALAKKLLGIIRSSVSSSSSLSQNLLLKALKYQGYEAQKA
                     RYREILKGRYKAMKKKLAEMTLPDGFTPMPFNSGYFMSFRCDGFSAEALRKKLLNEKG
                     IGTVSLMDRYLRVAFSSVEESDISDLCDVIAQAVVELR"
     gene            complement(4484..5674)
                     /locus_tag="PLS88_05170"
     CDS             complement(4484..5674)
                     /locus_tag="PLS88_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOU06766.1"
                     /translation="MKNAYSFPLENFISREKFERIKEFSRDKETPFLIIDLDIIKKNY
                     EDLKTYLPWATIYYAVKANPDDAVISLLRDLGSNFDVASRYELDQLLRLGISPDRMSF
                     GNTIKKEKDIAYFYEKGVRLFVTDSINDVDKLSRAAPGSKVFFRLLTEGLGADWPLSK
                     KFGSHPDLARQLIKTAIRFGLEPYGISFHPGSQQRDVGQWSSALTIAAQLFNWARHDL
                     KIDLKLINMGGGFPANYLDPTDPLQQYAEDIKRFLDNSFGLVWPEKIIIEPGRSMAGD
                     AGVIVTEIVNIAKKSVHDRYPWVFLDIGKFGGLIETLGESIKYPIYFEGQGSAEEIIL
                     AGPTCDSMDILYERTPYFMPSSAKVGDKAYILTTGAYTQSYSSIYFNGFPPLKSYILQ
                     SAEG"
     gene            5774..6547
                     /locus_tag="PLS88_05175"
     CDS             5774..6547
                     /locus_tag="PLS88_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06767.1"
                     /translation="MITFFIKKAFFDGWDNLYLLAALNGIFLGLLLLFLVVPIALAFP
                     IWLSIIIAGIGIFLLSIWDVVVTDTMYGIADGKSVHFADIKVAIKGSIKLGLLLGVVN
                     ILIAVAIMVAIPFYLGQKAVLGIFIGGLLFWMMVLAMLILQYVPAVFARERGTPRQAF
                     KTAIYLFIDNPGFSIFLFFWRILTFAISIFVVLLAPGPAGSSLASAVAVRLRMKKYSY
                     LNENPGANRRHIPWDDLLQEEKERVGKRTLKGMIFPWKD"
     gene            6580..7806
                     /locus_tag="PLS88_05180"
     CDS             6580..7806
                     /locus_tag="PLS88_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU06768.1"
                     /translation="MKPPSRDKSRGNQKKGHISQDSGQPLPEAFEERKKQALSSILYI
                     RFEGAEAQSRLPSGIDASIPYPVQLQTPTVKLDPSSITIESLLAGMLRVLAWDSENEN
                     ANKYRKYIKMSRPELFEELIMAGIQKAEAKDWIVAEEIFFAASGLEPEQPEPYINLAL
                     MHEEHAKLLFESDNEDESEKEDDLAYKYYQYLLGLKDVFAPAYYHAAFFFLRKHNYDK
                     AVSLLTSYIGMNDDEQRTRRAKSILEKLGSMGYLDTLFKEAYDFIQMGQEQKGLEKAE
                     QFVEKYPDVWNGWFLVGWANRRLGRWSEGAQAFRTALEKGADGADVYNELSICEVESG
                     ELETARSHLERALRIEPENEKIIVNLGALAFRQGHIEEAERFFRTAIELDPDDRIAKE
                     WLKKIESLRNSKGVGV"
     gene            7829..8875
                     /locus_tag="PLS88_05185"
     CDS             7829..8875
                     /locus_tag="PLS88_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin"
                     /protein_id="HOU06769.1"
                     /translation="MGNEFRNPKLPTAEELESAIEAGIATNIHIPTELRPSDPLHSLP
                     GGLIVLPPNKPCILVPDLHARPNFLYALLLTEFPDLGEPLLSALSNNRITLLFLGDIP
                     HAEGEEAARRWINSYDRALITRDARALLSPEMDEEMGLSLASLLKVMELQCRFPNSVF
                     CLKGNHDNMTNAADHGDFPFYKYADEGRMGAIWLSLRYNPYIMQKLRKYELSLAIAAA
                     DCNFCASHAEPAQAISRDSIIAYREHPRVVQALIWTANDEAEEGSVSKSLKAILGSDE
                     KARHSVWFSGHRPISGHFALRAKGQLVQIHNPSLWQIVFVDAESSKADFFLILPDQPK
                     PQKAASLILDPLAR"
     gene            8894..9721
                     /locus_tag="PLS88_05190"
     CDS             8894..9721
                     /locus_tag="PLS88_05190"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOU06770.1"
                     /translation="MAKRKKKRNQAKTGCLIWIGAFLILLILFLVKFEDIRTIVEKTG
                     FIDALNHAVSKQDSVPQQKPQGTKPEAPAPISELPLAAPPAEKPQIKSPIEPQETTTA
                     APENSKSPSVSPPEPEPIESSPGASVSKLPKEEQKMRTAVLYFVRIGDDGSISSQKVK
                     RTIPVSDSPLQDTLELLLKGPTESELRANLLSLIPADARLRSINTRGSTVILDFNDAF
                     AYNRYGKEGYIAQLRQIVYTLTEFQNIIDVQFLIEGKSRAFLSEGIALDKPWSRASF"
     gene            9806..10885
                     /gene="gcvT"
                     /locus_tag="PLS88_05195"
     CDS             9806..10885
                     /gene="gcvT"
                     /locus_tag="PLS88_05195"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOU06771.1"
                     /translation="MHDWHVSHGAKMAPFAGYDMPINYPTGAIEEHLITRRGVGLFDI
                     DHMGQIEIAGEAAETFVSHMISAKVSDLVAPMARYSLLLNEKGGIIDDLFVYRLPNSW
                     WIVVNASNRAVDLEWFRSKAPTNVSIIDRSDDTYMIAVQGPRAIELLDKISNTPVSPI
                     PRFCWANISIEGIPILFGRTGYTGEDGGELFFPAKKAIELWEYLLECGESFGIETKPI
                     GLAARDSLRFEAGLPLHGHEIGIDINPIEAGFKWACDFEKEFIGKEALLSIIEQGISR
                     KLVGIEVFGGVAREGYEVATADGVIIGRCVAGMYCPTVKKYAANAFVNPNYAKIGTNL
                     SVIIRGQPRSAEVIKRPFYVPVYRR"
     gene            10943..11326
                     /gene="gcvH"
                     /locus_tag="PLS88_05200"
     CDS             10943..11326
                     /gene="gcvH"
                     /locus_tag="PLS88_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOU06772.1"
                     /translation="MAIDKNARYLESHEYAKPEGELMVIGISDHAQAELGDVVFVELP
                     NVGKELDKGVVFGTIESVKAASDLYMPISGKVVEVNEEVKNDPSLVNKDCYGSGWLIK
                     VAPTKLSEFESLLDAESYGTSIGEV"
     gene            11331..12650
                     /gene="gcvPA"
                     /locus_tag="PLS88_05205"
     CDS             11331..12650
                     /gene="gcvPA"
                     /locus_tag="PLS88_05205"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOU06773.1"
                     /translation="MSFVPHTDPERAAMLKGIGVSSIEDLFSDIPEHLRIEGLALPGA
                     VSEIEAIDEVETLADRNVQTKDMDWFLGAGAYYGYIPAVVPALAGRGEFLTAYTPYQP
                     EVSQGTLQAIFEYQSMTAKLLGMSVVNASHYDGATALAEAVLMAWKAKEGRNRIFLPK
                     DLHPEYSQVIATYCTSFDIEFIRYEGDPAQAPIDERTAAIIIAYPGFSGEMYPISKAS
                     EKAHEKGALCIVQADPLMCAIFKSPGEQGADIVTAEGQCLGNPLNFGGPYLGMMGTTD
                     ALIRRMPGRIVGQTKDKFGKRGFVLTLSTREQHIRREKAVSNICSNQGLSMLQTCIYL
                     ATLGKNGLQEVAKQSYDKAHYAAQLIDIIPGYHVVPKLFFREFLVETPDSATKIVEAL
                     NKKSIMPGLALSHYYPERANELLICVTEMNTKAQIEKLASALKEAVK"
     gene            12647..14089
                     /gene="gcvPB"
                     /locus_tag="PLS88_05210"
     CDS             12647..14089
                     /gene="gcvPB"
                     /locus_tag="PLS88_05210"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOU06774.1"
                     /translation="MNAMNMRDEPLVYELSREGRIGCSLPRSDVPSKEIPLEIARSEL
                     DLPELDELTVVRHFTRLSQKNFSIDTEFYPLGSCTMKYNPKANEVVASNPGWKNLHPL
                     VGEEYAQGAIELLWRLQEGLREVGGFAAASLQPAAGAHGELSGVLMIHAALKERGETK
                     RKKMLIPDSAHGTNPASCTMAGFTTQTIPSGLDGGVDMEALRAALDDTIAGIMITNPN
                     TLGLFEKNIEDICRLVHEAGGFVYGDGANMNALTGVFKPGRSGIDVMHFNLHKTFSTP
                     HGGGGPGAGMVAANETLAPYLPGPIAVKADSVYRLEMPAKSIGRVKAFHGNFGVLVRA
                     YCYLLMTGSDGLRQVAENAVLNANYLKSLLEKVYPVPYKRHCMHEFVAKGAIADTVHT
                     LDIAKRLIDYGFHPPTIYFPLIVQEALMIEPTETESKETLDLFAEAMKRIAEEASKNP
                     ELLHDAPHIAPVARLDEVMAARYPILCYRG"
     gene            complement(14221..16491)
                     /locus_tag="PLS88_05215"
     CDS             complement(14221..16491)
                     /locus_tag="PLS88_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06775.1"
                     /translation="MTERKLFLLFEALIFIAAVLFTACPTGIPKGTAHIYVRFVLPGD
                     LTNASAKKSGSTIIGPESGWNIVSYRLTFTGDNGEVITKVIDKASGELSIKAGNWILL
                     AEGLTANGTVIVQKETQVLAQAGKRVNIQIMLHLAQGKGSLQLEFSPNQPLPLDWKYS
                     VSLEYRGLPGDPTFQGPSLLSTDVPATQMHYQVDELQSGNYSLAVQVRDTSSASIAGC
                     IVTVLVLPEQTSIGSCSIYLPDPSATISLSTPKLELSANAAISVERYLNRNKSVLVPI
                     TVAEGETNIDVEWYQNGAKIEKAQEEPTQCLPGHKIFLAPSEELDSQMRVSMDALLSD
                     RNSGISQAFSNSSSLLTGPLSDQIEWVQSIDYKAALSPSLHNRNNPENAGTGSLHEVK
                     CVATSSSGLIAVAGLDESNALHLFYSPSGKQAALGGSNYEIPSSVGWIRLWRDKIFID
                     KSEKNPDIAAVSLDGSLIAASASTGNWIRLYRLDNSGSIASKIDIVSSCDEASKFEKI
                     KAMRFTADSTRLFILSDDPEKILVFNIEKLFSGENSCEKEFSFVVSSQTEPGPSLQSS
                     SKKDSSNFMMRDMMLLDDGWIALCSSEIKNVFFIRYSLADCSFSCETKIEPVPNSKPL
                     GEPRSIAFDENSERCYVICNNGKLLLFEKPDPQSSYALSMTFRLSPDLDKANSLELVK
                     KSSGGTYLVTGGGNGLGIISLDANGHPISQSSLNSQEENPYGIKSINNVAVLGESIIS
                     AGGDSGIVAMFDILGS"
     gene            16683..18155
                     /gene="glgA"
                     /locus_tag="PLS88_05220"
     CDS             16683..18155
                     /gene="glgA"
                     /locus_tag="PLS88_05220"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOU06776.1"
                     /translation="MKILFISSELTPFAKSGGLGDAVSSLAAALSKLGHDVRVLLPRY
                     YFISKNTLRRINGLLEIDSARERHRAFLYTANLPNSQAKVYLLECDSLYGRNGIYGYS
                     SNQEFPDNPERFSVLSKAAFALCRNLGWIPDIMQAHDWPTAPVPVYLNTIEKNTEFSG
                     TAGVLTIHNLGYQGIYSRDHFAELGLGWEYYYGAGFEYYGNINFLKAGIVSAECIATV
                     SPTYAMEIQTPSGGFGLDGLLRQRSRDLVGILNGIDTEVWNPLTDPFLPAHYSAGDIS
                     GKAICKEALQKELGLPIDPQAPLIGMVTRLVEQKGISEVFGRTYGCMRKILETIHVQV
                     AVLGSGDAWAEEAIMNFSAQYPQFKGVIGYNERLAHLIEAGSDFFLMPSRYEPCGLNQ
                     MYSLVYGTLPIVHRTGGLADTVVNYNQDLGDGTGFMFDDLTPQAVYDTVGWAMWAWYN
                     RPEHIKTMRERAMKQDFSWNRSAEEYGRLYQHALALRKAK"
     gene            18201..>18663
                     /locus_tag="PLS88_05225"
     CDS             18201..>18663
                     /locus_tag="PLS88_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06777.1"
                     /translation="MSESKILDYNKQEVRDQLLHALRSYTKEWTSADLARATGLPLAQ
                     INAEMPTISDEYRGRIRVSERGDLLYSFPNGFKSKYKGFGPSMRRLWKTITKGAVETG
                     KFLFKVWILVTLFGYFFIFLALALVALFGSVAMRGGGSRDSDDRRGGGGLGG"
BASE COUNT         5223 a         3963 c         4406 g         5071 t
ORIGIN      
        1 tgcagcgata acgaggtaca ttgccaaaac atttggtatg atagaagcct agccttaaaa
       61 accagcgacc ttttggagca gccttatgat agagacaggc tggatttggg cagaaaggaa
      121 aagaatgcat gtttacctcc atacattctc tttatcgctc tcttccctac tttatgcttc
      181 aaattcttat acaaggttcg tagcagtaat gatcttttca atcacttctg tgctggattc
      241 tgaatttctg aaggttcacc ctttgtaacc ttacccataa ttgcgcgagc gcagagcata
      301 cagagtgtgc agaagaggaa aattccctga tatccgaaga catcgattat ggctccagtc
      361 aatacagggc ccaaaattgc agcagcttgg cttgcggtat aataaaggcc tgtatagatg
      421 ccaatagtac cccattctgc catctgccat agcatgggga aagaatttgt tattatcgat
      481 acccaaaaaa tcccgaaaat aaacatgatt gcccagaatg aggccaatct caaggagttt
      541 ggtagactag cggtaatcca tttatgagca aacagtagaa gcgtgagtat cgatattacc
      601 agcaaacatg cacggattgt cttttttcta ccaaagcggt gtgcgacata acccgaaggg
      661 atagcaaata tagcataagc tatacccacc attcccgctg ccagagctgc agtgccactg
      721 gaagtcttga gcacatccac cgaatacttc cctatgaatg gtaagacacc ttgatatcct
      781 agaaaccata gaaatattgc gattagaata tatagtgcgc tcttatcctt ctgtgctgcg
      841 acatttcgaa aactctcgag aatgggcact ttttgttctt gtacttctga aggatctggc
      901 tgcttctcac gaataaatat aagaagaagg atcacggcga gaatgacgaa cattccacca
      961 agagggaagg gtagcctatc cttggtagaa ccaaggatag gaagggttgt atctagatcc
     1021 attagtctag caagcccaat cgttcctaca atgcttgcta taccgcccat tgtgttgata
     1081 acgccattcg cttcggagcg gtaatccgct ggtatcgtat ccggcataag cgcaaccacc
     1141 ggtccacgca cagactgctt tgtcacattg agaagaatca atgtaacgag caaggctacg
     1201 agagatgctt ctgcggcata gggtatggcg ccaaaaagca aagcagtaag gggtaaaagg
     1261 acgagaatgt agggcatacg acgcccaagc ttcgtatatg tatgatcgga ccaggcgctc
     1321 acaagcggta ttagaaagat agctaagata ttgtcgagcg tcatgaaaaa gccgacaagg
     1381 ctcattttgc ttaggtatct gctgaggaat atcggcacat aggtatcgta tagtggatcc
     1441 ataaggccca tggtaaaaaa gccgaatccg ataagaaaag ttatgcccaa ataaggtttg
     1501 aatccctttt tctcgctcat catagccctc cttgtgagta ttttattctt ttcaatgcat
     1561 aagaacctta catacaccaa atggtcgaat attaatatgt tgtacgcagc cctctccaaa
     1621 gacggtctcc atgcgctcgg taaattcagg gaatcttgat tccggaacat aagcctgaat
     1681 tgagcctgca aaaccaccac catgtacgcg ccaggcgcct tctgggctca acaggtcctc
     1741 gcttagcata agagcgatag aaagactctg ctggtgcggg ttgccagtgt gtatattctg
     1801 aaggtaatgc caactcgatc ttccggaatc atttacaatt gacagatagg cagatatatc
     1861 gtttctgtta agggcttcca ccatttttac aggacgagag ttttcatgta caaagtgcca
     1921 ggcgcgcaat aatgctctat ccccgcatcg tatgcgaatg tccgaaagtt tggctaagag
     1981 atcctgcttt tcaatccctc gcatagcagg ccttccaaaa aggtctgcaa cgcatttcat
     2041 ttctttggga atgtctgaat attctattgt aagatcctcg tgattcgagc ctgtattgac
     2101 aatggccaat ataaggccat tagatgcaaa atcgtatgtc agtttttcaa tctttggctt
     2161 tccaggttca tataaatcta taaatacaat gccaccttcc gcgcatgcaa tttgatccat
     2221 aaggccgcaa ggctttccga agtattcatt ttctgcccat tgtcctgcaa tagcccaatc
     2281 gatcggactc atttcatact tgcccatgtc gtcgaatatt gctgcaatca aaagctcaaa
     2341 ggctgcggag ctcgatagac ccgagccagc tggtactcta gagtccattg caatatcgaa
     2401 accatggcca atttgcatac ctttgtgagt cagccaagct gcaaggccgc gtatgatcga
     2461 ggccggcgag cctctttctt ctatcttgaa ggaggtatct ctcaggtcga tccttattgg
     2521 agagtagcct tcagaggcaa gagaaatgag gtaatcgctg cgtggactgg ctacagcgag
     2581 catgtcgaga tctactgagg cggcaagtac gcagccatta ttatggtcgg tatgattgcc
     2641 gcctagctcc gtgcggccag gggaagaaaa ataatgtaat tcttggcgac agctgctttc
     2701 tagaggaggt aacctagata caagagattc atatctagtc ttgtgatttt ctgtttcgcg
     2761 attctgaaat gaggcaagag gaatgcaagg gatcttctgc tgtaccatag tatgctcttg
     2821 cccattgtcc tagaaaatcg aggcaaagtg aagagccaca ttcaatatat caagagataa
     2881 cgcagagaaa gtgtcgataa gacataggtt cttttccgtg gtctcttcct cctttaaaaa
     2941 tgttatcatt aaaacatata gagcaccttc ggctacacag gcaatatatt tcctaattga
     3001 tatataaaat atatttatat ctatgttaat agtgtcttat ttaattcaca ttgcagtatg
     3061 tatagaacgg tgttaaaatc aaaaatagat tttcacaaat cgataattca agctcgagag
     3121 gttaatatga atcagctggc agaagaattg aatgctgtcc ttgcgggaac tgtagccgag
     3181 aggctatttt ctgaaatggg aaggcgaatg tattttccta aagggatcat cacccagagt
     3241 gccgaagcag gagaaaaagc ttatagattc aatgctacta ttggcatggc ttatgagcat
     3301 ggccagccaa tgatgctaga tgctttgcgt gccgaattgc caggtttgac tccaacagaa
     3361 gcagttgctt atgcgtctac aggcgggatc ccggagcttc gtaagacctg gaaagaatcg
     3421 ctctatagaa agaatccctc gctcaatggt aaaaagatat ctcttcctac agtcgttcct
     3481 gggcttacag cagcgatatc ctatatctgc gatatgtttg tagagcctgg agatacagta
     3541 attctacctg acctgcactg gccaaactat aggctcattg tagaagaaag aaaaagtgca
     3601 gcaggcatca cctatccact atttgctgga aaaggctaca atatcgaggg cttgctcgaa
     3661 ggtgtaagac aggcgagtca gcgtcggggc aaggcgataa ttatccttaa ttttccgaat
     3721 aatcctactg gctattcttt gacactcgat gaggctgatc gcgttacagc tggacttttg
     3781 gagatcgcgc agcaggggac agatatcctt gtgatagccg acgatgcata tttcgggctg
     3841 caatatgaag acgacctact ctgtgaatct atctttgcga gattggctga ccttcacccg
     3901 aatatactcg ctataaaggc cgatggacca acgaaagaag attatgtatg gggattccgt
     3961 ataggctttg tgacctttgg tgccgcaggt tttggtgatg agcattacga agctcttgcg
     4021 aaaaagcttt taggaataat acgttcaagc gtctctagtt caagtagtct ttcgcagaat
     4081 cttcttctaa aggctcttaa atatcagggc tatgaagcgc aaaaagcaag gtatagggaa
     4141 attttaaaag gccgttataa ggctatgaag aagaaattag ctgagatgac cctgccagat
     4201 ggctttactc caatgccatt caactcaggc tattttatga gtttccgctg cgatggtttc
     4261 tctgccgaag ctcttaggaa gaagcttttg aatgagaagg gaatcggaac tgtgtctctt
     4321 atggatcgct atcttcgcgt cgctttctcg agcgtggaag aaagcgatat ttcagacctc
     4381 tgcgatgtaa tagcacaagc agttgtcgag cttcgctagc aagactgaga aaaattgtac
     4441 cgaaaagtgc gcagaactat gtctatctag cagtaatgct ctattagcct tcagctgact
     4501 gcaggatata ggatttcagt ggcgggaagc cattgaaata aatagatgaa taactctgag
     4561 tgtatgcgcc tgtggttaga atatatgcct tatctcccac cttggctgaa gatggcatga
     4621 aataaggggt tcgctcatag agtatatcca tagagtcaca ggtaggtccc gcaagaatta
     4681 tttcttcggc cgagccctgg ccttcaaaat agatcggata cttgatcgat tcacctagtg
     4741 tctcgataag ccctccgaat ttgcctatat cgagaaaaac ccatggatat ctatcgtgga
     4801 cggatttctt agcgatatta acgatctccg tgacaatgac gcctgcatct ccagccatgg
     4861 aacgccctgg ttcgattatg attttttctg gccataccaa accgaagctg ttgtcgagaa
     4921 aacgctttat gtcctcggca tattgctgca gcggatctgt tggatctaga taattggctg
     4981 ggaaacctcc tcccatattg atcaacttga gatcaatctt gaggtcatgg cgagcccagt
     5041 tgaagagctg ggcggcgata gtgagtgcgc tcgaccactg acctacatcg cgctgctgac
     5101 tgccagggtg aaaagagatc ccatagggtt caaggccaaa acgaatcgcg gtctttataa
     5161 gctgacgagc aaggtcgggg tgagaaccga atttttttga tagaggccag tcggcgccta
     5221 ggccttccgt gagaagtcta aagaacacct tgctgccggg tgctgcgcgg gaaagcttat
     5281 cgacatcgtt tatcgaatct gttacaaaga gcctaacgcc tttttcatag aagtatgcga
     5341 tatctttttc ttttttaatg gtgtttccaa agctcattcg gtctggagat atgcctaacc
     5401 tcagcagttg atcaagttca tagcgagagg ctacatcgaa gttagagcca aggtctctca
     5461 agagggaaat gactgcatca tcaggattag ccttgacagc ataataaata gttgcccatg
     5521 gaaggtaggt tttgagatct tcgtaattct tttttataat gtccaagtct atgatcaaga
     5581 acggggtttc tttatcccta gagaattcct tgattctctc gaatttctca cgacttataa
     5641 agttttcgag gggaaaggaa taggcgtttt tcactggcct gacctcttcg atttgagata
     5701 gctctttaga gctccaagcg catacattat gcatctactt gtccaaaggt caagcatgga
     5761 gtatagaaat agtatgatta cattttttat aaagaaagcc tttttcgatg gctgggataa
     5821 tctatatcta ttggcagcct tgaatgggat ttttcttggc cttcttcttt tattccttgt
     5881 agtcccaatt gcgctggctt ttcctatttg gctttctata attattgctg ggattggaat
     5941 ttttctactt tctatatggg atgtagtggt gaccgataca atgtatggca tagccgatgg
     6001 aaaaagcgtc cattttgcgg acataaaggt tgccattaaa ggatcgatta agcttggctt
     6061 gttgcttggc gtagtcaata ttttaatagc agtagccata atggtagcta tccccttcta
     6121 tcttggccag aaagcagttt taggtatctt tataggaggc cttctattct ggatgatggt
     6181 tttggccatg ttgattttgc agtatgtgcc agcagtcttt gcacgcgaaa gaggtactcc
     6241 ccgccaagct ttcaagacag caatttatct ttttatcgat aatccaggct tttcaatatt
     6301 cttgtttttc tggagaattt taacattcgc tatatcgata tttgtggtat tgctggcccc
     6361 tggacctgct ggaagctcac ttgcatccgc agtagcggtg aggcttagga tgaaaaaata
     6421 tagctatcta aatgaaaatc ccggagcaaa cagacgccat ataccatggg atgatcttct
     6481 acaggaagag aaagagagag tcggcaagag aacgttgaaa gggatgattt tcccctggaa
     6541 ggattgatca tgatcgataa atcgaggaga gaaccaggca tgaaaccgcc atcgcgagac
     6601 aagagccgag gaaatcaaaa aaaaggccac ataagtcaag atagtggaca acccttgccc
     6661 gaagctttcg aagaacgcaa gaagcaagcg ctttcgagta tcttatatat ccgcttcgag
     6721 ggtgctgagg cacaatcgcg cttgccatca gggatcgatg ctagtattcc atatcctgtc
     6781 cagttacaga ctcctacagt caagcttgat ccttcatcta tcactattga aagccttttg
     6841 gctgggatgc tgagagttct tgcttgggat tcagaaaacg aaaatgctaa taaatatcgg
     6901 aaatatataa agatgtctcg tcccgagctt tttgaagagc taataatggc tggaattcag
     6961 aaagccgaag caaaagattg gattgtcgct gaggaaatct ttttcgcagc aagcggcctt
     7021 gagccagagc agccagaacc atacatcaat cttgcgctga tgcatgaaga acacgcgaag
     7081 ttgctttttg aatctgacaa tgaagatgaa tctgagaaag aagatgatct ggcgtataaa
     7141 tactatcagt accttctagg gcttaaggat gtttttgctc ccgcgtatta tcacgctgcc
     7201 ttctttttct tgagaaagca caattatgac aaggctgttt ctctattgac aagctatatc
     7261 gggatgaatg atgacgagca acgtactcgc agagccaagt caattcttga aaaacttggc
     7321 agtatgggtt atctcgatac gcttttcaaa gaagcatatg atttcattca aatggggcaa
     7381 gagcaaaagg ggcttgagaa agcagaacag tttgtcgaaa agtaccccga tgtatggaat
     7441 ggctggtttc tggtgggatg ggcaaatcgt aggttggggc gatggtcaga aggtgctcag
     7501 gcatttcgta ctgctctcga aaagggagct gatggggcag atgtctacaa tgagctatcg
     7561 atctgtgagg tagagtcagg tgagcttgag actgctaggt ctcatctcga aagagccctg
     7621 agaatagaac cagaaaatga aaaaataatt gtaaatctag gggctctcgc tttccgtcag
     7681 gggcatatag aggaagcgga aagattcttc cgtactgcaa tcgaactcga tcctgatgat
     7741 agaattgcca aggaatggct caaaaagatc gaatctctta ggaatagtaa aggggttgga
     7801 gtctaagagc agtgttgtcg agatgattat gggtaatgag tttcggaatc ccaagctccc
     7861 tactgccgaa gagcttgagt cagcaataga ggcggggatt gcgacaaata tccatattcc
     7921 tactgaattg aggccctccg acccactcca ctctctgcca ggaggcttga ttgttctgcc
     7981 acctaataag ccttgcatcc ttgtacctga tctgcatgca cggccaaact ttctttatgc
     8041 cctactccta actgagtttc cagacctagg tgaaccattg ctttcagcgc tctcgaataa
     8101 tcgtataacg cttctgtttc tgggagatat tccgcatgca gagggagaag aagctgctag
     8161 acgctggatt aactcttacg atagagcttt gattactcga gatgcaagag cacttctttc
     8221 gcctgagatg gatgaagaga tgggtctctc tctggcttca ttgctaaaag ttatggaact
     8281 acagtgtcgt tttccaaatt cggtattttg cttaaaggga aatcacgata atatgacgaa
     8341 tgctgcagat catggcgatt ttccatttta taaatacgca gatgagggca ggatgggagc
     8401 catctggctc tcgcttcgat ataacccata tataatgcaa aagctgcgga aatatgagct
     8461 ttctctggca atagcagctg cggattgtaa tttttgtgca agccacgccg agccagccca
     8521 ggcaatctcc agggattcca taattgcata ccgagaacat ccaagagtcg ttcaagcgct
     8581 tatatggact gcaaatgatg aagctgagga aggctcggta agcaaatctc taaaagcaat
     8641 tctaggatcg gacgagaaag ctagacactc ggtttggttt tcaggacata ggcctatatc
     8701 ggggcacttt gcgcttagag caaaagggca gcttgtgcag atacacaatc catcgctgtg
     8761 gcagattgtc ttcgtagatg cggagtccag taaagccgat tttttcctaa ttctgcccga
     8821 tcaacccaaa cctcaaaagg cagccagtct tatccttgac cctttggctc gctaaaagta
     8881 ggatcaatac aatatggcga agcgaaagaa aaagcgtaac caagcaaaga caggttgtct
     8941 aatctggatt ggagcgtttc ttatcctatt aattctattc cttgttaaat ttgaggatat
     9001 tcgcacaatt gttgagaaaa caggcttcat agatgcgctc aatcatgctg tatccaagca
     9061 agattctgta cctcagcaga aaccgcaagg aacaaagcca gaagcacctg cgcctatatc
     9121 ggaactgccg cttgcagcac cgcctgcgga aaaaccgcag attaagagcc caatagaacc
     9181 gcaagaaaca actactgcag ctccagagaa ttccaagtca ccttctgttt caccaccaga
     9241 accagaaccg atagagtctt ctccaggagc gtctgtctct aagctgccca aagaagaaca
     9301 aaagatgcgg actgctgtgc tgtattttgt gcggatagga gatgacggct cgatttctag
     9361 ccaaaaagta aaacggacaa tacctgtctc ggatagtccc ttgcaagata ctcttgaatt
     9421 gcttttgaag gggccaacgg aatcagaatt gcgggcaaat cttctatcgc taattcctgc
     9481 agatgcaaga cttcgaagca tcaatacgag gggcagtacc gttattctgg atttcaacga
     9541 tgcatttgca tacaatcgtt atggaaaaga aggatacata gcccaactta ggcagattgt
     9601 atatacgctt acagaatttc agaacataat cgatgtccag ttcctaatag aagggaaatc
     9661 tcgtgctttt ctttccgaag gcattgctct cgataaacct tggtctagag ctagtttcta
     9721 aaagctcgtt gtcagtattc atgagtgatg ttagaataaa tgaagtattt cataatatga
     9781 ggaggagcct acgaatacca cagcattgca cgattggcat gtctcccatg gcgctaagat
     9841 ggcgcctttt gcaggatatg atatgccgat caattatcca actggtgcca tcgaggagca
     9901 tcttattaca cgacgaggag ttggtctctt tgatatagat cacatggggc agatcgaaat
     9961 agcaggtgag gctgcagaga cttttgtctc tcatatgata agcgcaaaag tctccgatct
    10021 tgttgctcca atggctcgat attcgctcct tctcaatgaa aagggcggaa tcatcgatga
    10081 tctcttcgta tatagattgc cgaattcgtg gtggatcgta gtgaatgcat caaatcgggc
    10141 agtagatctt gaatggttta gatcaaaagc tcctacgaat gtttctatta tagatcgttc
    10201 ggatgacaca tatatgatag ctgttcaagg cccgcgagcc atagaactgc tggataagat
    10261 ttccaatact cctgtttcgc caatcccccg cttttgttgg gcgaatattt ccattgaggg
    10321 cattcctatc ctttttggcc gtacaggcta tactggtgaa gatggtggag agcttttctt
    10381 ccccgcaaaa aaagccattg agctttggga ataccttctt gagtgtggag aaagctttgg
    10441 catcgaaacc aaaccaattg gacttgcggc aagagatagt ctgcgatttg aagcaggatt
    10501 gccgcttcat gggcatgaga ttggtatcga tatcaatcct atcgaggctg gtttcaaatg
    10561 ggcttgtgat tttgaaaaag aattcatagg aaaagaggct ctgctttcta tcattgaaca
    10621 aggtattagc cgaaagcttg ttggcattga ggtatttgga ggcgttgcgc gtgaaggcta
    10681 tgaggtcgct acggccgatg gtgttattat cggtcgttgt gttgcgggaa tgtattgccc
    10741 aacggtcaag aaatatgcgg ccaatgcctt tgttaatcca aattatgcca agatagggac
    10801 aaatctatcg gttataatac gtggccagcc tcggtcagcc gaagtaatca aacgcccctt
    10861 ctatgtgcct gtctatcggc gctagaaccg ttgtacgcac tgagcgtctt gcaatatata
    10921 atttctctaa agggaggaaa atatggcgat cgataagaat gctaggtatc ttgagtccca
    10981 cgagtacgca aaaccagaag gagagctcat ggtcataggg atttctgacc atgctcaagc
    11041 tgagttgggc gatgtcgtgt ttgttgagct tcctaacgtg ggaaaggagc tcgataaggg
    11101 ggtagtattt ggaacaatcg agtcagtaaa agcagcgagc gacctttata tgcctatttc
    11161 tggcaaagta gtggaagtca atgaagaagt gaaaaatgac ccttcgctcg taaataagga
    11221 ctgttatggt tctggatggc ttataaaagt ggctccaacc aaactttcag aattcgaaag
    11281 ccttctcgat gcagaatcct atggcacatc cataggggag gtgtgacatc gtgtcctttg
    11341 ttcctcacac tgatccagaa agggctgcga tgctgaaagg catcggagtc tcgtctatag
    11401 aagatctgtt ctctgatatt cctgagcatc tgcgaataga aggtttagcg cttcctgggg
    11461 ctgtatctga gattgaagcg atagatgaag ttgagactct cgcggaccgc aatgtccaga
    11521 caaaagatat ggattggttt ctaggagctg gcgcatatta cggctatatt ccagcagttg
    11581 tgccagcctt agcaggcaga ggtgaatttt taacagccta tactccctat caaccagaag
    11641 tatctcaggg tacattgcag gcgattttcg aataccagag catgactgct aagctcttgg
    11701 gaatgtctgt agtcaatgcc tctcattacg atggagctac agcgttggct gaagcagtac
    11761 tcatggcatg gaaggccaaa gaaggtcgaa atcgtatttt tctaccaaag gatcttcatc
    11821 cggaatattc tcaagtcatt gcaacctact gcacttcctt cgatatcgaa ttcattagat
    11881 acgaaggaga tccagcgcag gcaccaatag atgagagaac agctgcaatc atcatagcct
    11941 atccgggttt ttcgggagag atgtatccca taagcaaagc atctgaaaag gctcacgaaa
    12001 aaggggcact ctgtattgtt caagctgatc cccttatgtg cgcaattttc aagagccctg
    12061 gagagcaggg tgccgatatc gtaacggccg aagggcagtg ccttggtaac ccattgaact
    12121 ttgggggacc ttatttagga atgatgggaa cgacagatgc gctgataagg cgtatgccag
    12181 gcaggatcgt cggtcagaca aaagataagt ttggcaaacg tggttttgtc cttacactgt
    12241 ccacgcgaga acagcatatc agaagggaaa aagcagtctc caatatatgt tcgaatcaag
    12301 gcttgagcat gctgcagacc tgtatctatc tagcgactct cggcaagaat gggctgcagg
    12361 aagtagcaaa gcaatcctac gataaggctc actatgctgc tcagcttatc gacattattc
    12421 ccggatatca tgtagttccc aagctcttct ttagggaatt cttagtcgaa acaccagatt
    12481 cggctaccaa gatcgtagaa gcattgaaca aaaaaagcat catgccaggt ttagcccttt
    12541 ctcactacta tccagagagg gcaaatgagc tcttgatctg tgttactgaa atgaatacaa
    12601 aagctcaaat agaaaagctc gcttctgcgc tgaaggaggc cgttaaatga atgctatgaa
    12661 tatgcgcgac gagcctttag tctatgaatt gtctagggaa ggaagaattg gttgttctct
    12721 tccaagatca gatgttccca gcaaggaaat acctctagag attgcccgct ccgaattgga
    12781 tctgccagag ctcgatgagc ttacggtagt gagacatttc acaagacttt cccaaaagaa
    12841 tttctcaatc gatacagaat tctatccgct cgggtcttgt actatgaaat acaatccgaa
    12901 agccaatgag gttgttgctt cgaatcccgg atggaaaaat ctgcaccctc ttgttggaga
    12961 ggaatatgct caaggagcta tagagctatt atggagactc caggagggac ttcgggaagt
    13021 aggtggtttt gctgcagcct ctcttcagcc tgctgctgga gctcatgggg agctttcagg
    13081 cgtgctcatg attcatgctg ccttgaaaga gcgaggagag acaaagcgaa aaaagatgct
    13141 cattccagat tcagctcacg ggacaaaccc agcaagctgc acgatggctg gatttacaac
    13201 tcagactata ccttcagggc ttgatggcgg tgtagacatg gaggcgcttc gcgccgctct
    13261 cgacgataca atagccggaa taatgatcac taatccgaat acccttgggc tttttgagaa
    13321 gaatatagag gatatttgta ggcttgtgca tgaggcaggt ggctttgtat atggcgatgg
    13381 agctaacatg aacgctctta ctggagtttt taagccaggc agatcgggca tcgatgtgat
    13441 gcacttcaat ctacacaaga ctttttctac accacatggt ggcggcgggc caggtgcagg
    13501 aatggtagca gccaatgaga ctctagcgcc atatcttcca gggcctatag ccgtcaaggc
    13561 agattctgtc taccgtcttg aaatgccagc gaaaagcata ggcagagtta aggctttcca
    13621 cgggaacttt ggtgtcttgg tgcgcgcata ctgctaccta ttgatgaccg gttcagatgg
    13681 acttcgccag gttgccgaga acgcggtgtt aaatgcgaat tacctcaagt cactcctgga
    13741 gaaagtctat cctgttcctt acaaaaggca ttgcatgcat gaattcgttg ccaaaggagc
    13801 gattgccgat actgtgcata cattggatat tgcaaagcgc cttatagact atggcttcca
    13861 tcctccaact atttatttcc cgctcatcgt acaggaagct ctgatgatcg agcccacaga
    13921 gacagagtcc aaggaaactc tcgatctttt tgctgaagcg atgaagagaa tcgcagaaga
    13981 ggcgagtaag aatccggagc tgctacatga tgcgccacat attgcgcctg tagctcgtct
    14041 agatgaggtg atggcagcgc gatatcccat actttgctat cgcggctagg atttctattt
    14101 caagagacga ataccaaaag agaaagactt ggtcagagtc tttctctttt tttaattccg
    14161 cttgggcggt ttctatttcc attttttacg caaatcgtgg ttttttggaa ttgttttgcg
    14221 tcatgagcct agaatatcga acatggctac aatgccacta tcgcctcctg ctgagattat
    14281 ggattctcca agaactgcga cattgtttat tgattttatt ccatagggat tttcttcttg
    14341 cgaattcagc gaggactggc ttattgggtg cccattagca tccagcgaga tgattccgag
    14401 accattaccc cctccagtga ctagataggt tcctccagag ctctttttga caagctcaag
    14461 cgaattggct ttatccagat cgggagatag tctgaaagtc atggatagag cataggaaga
    14521 ctgaggatct ggtttttcga acaaaagcaa ttttccgtta ttacaaatta catagcatcg
    14581 ctcactgttc tcatcaaagg cgatcgatcg tggttctcca aggggctttg agtttgggac
    14641 aggttcgatt tttgtctcac acgaaaaaga acagtcggcc aatgaatacc ggataaaaaa
    14701 tacattcttt atttcagagg aacacaaagc gatccatcca tcatccaata acatcatatc
    14761 tcgcatcata aaattagaag agtctttttt agaagagctt tgcagagaag gcccaggttc
    14821 agtttgtgag ctaactacaa aagagaattc cttttcgcaa ctattttctc cactaaagag
    14881 tttttctata ttgaagacaa gtatcttttc aggatcatcg gataaaatga atagccttgt
    14941 ggaatcggct gtaaacctca tagctttaat tttttcaaat tttgaagctt cgtcacagct
    15001 actgacaata tcgattttgc tagcaataga gccagagtta tccagtctat agagccttat
    15061 ccaattgcct gtcgatgctg aagccgctat cagcgaccca tccagagata cggccgctat
    15121 atccgggttc ttttcgcttt tatcgataaa tatcttatct cgccacaggc gtatccaacc
    15181 aacagaagaa gggatttcgt agttgctgcc tcctaatgca gcttgtttcc cagatggaga
    15241 atagaaaaga tgaagagcat tggattcgtc gaggccagcc accgctatca atccagaaga
    15301 actagttgct acgcatttca cctcatgcaa actacctgta ccggcatttt caggattatt
    15361 tctattgtgg agcgaagggc taagtgctgc cttatagtcg atactctgca cccattcgat
    15421 ttgatccgat aagggtcctg taagaaggga tgagctatta gagaatgctt gcgaaatgcc
    15481 cgaattcctg tcgctaagaa gcgcatccat agagacgcgc atctgagaat ccagttcttc
    15541 gcttggtgca aggaagattt tatgacctgg gagacattga gtcggttctt cctgagcctt
    15601 ttcgatcttt gctccgtttt ggtaccattc gacatcgata ttggtctctc cttccgcaac
    15661 agttataggc acaagaacag acttatttcg attcaagtat cgctcgacag aaatcgcagc
    15721 attagctgat aattccagtt ttggagtgct tagcgaaatg gtcgcggacg gatctggaag
    15781 atatatacta caagagccta tggaagtttg ttctggcaag acgagcactg tcacaatgca
    15841 gcctgctatc gaagcagaag aagtatctct aacttgtacc gcaaggctgt aattccccga
    15901 ttgcaattca tccacctgat aatgcatctg ggtagcagga acatctgtcg acaaaagaga
    15961 tggcccttga aaagtaggat ctcctggtaa gcctctgtat tcaagcgaaa cagaatactt
    16021 ccagtcgagg ggaagaggct gatttgggga aaattccagc tgcagagagc ctttcccttg
    16081 ggctaagtgc agcattattt gtatgttgac cctctttcct gcttgagcta gaacttgtgt
    16141 ttctttttgg actattacag tcccattggc agtaaggcct tctgccagaa gtatccaatt
    16201 gccggccttg atgctcagtt ctccgctggc tttatcgata actttagtta taacttctcc
    16261 gttatcgcct gtgaatgtca aacgatagga aactatattc catccggatt cggggcctat
    16321 tatggttgat ccagatttct tagcactcgc gttcgtcaaa tcacctggca acacgaatcg
    16381 tacatatata tgtgctgtac ccttaggaat tcctgtaggg caagctgtaa acaacacagc
    16441 tgcaataaaa atcaaggctt caaacaataa gaataacttg cgctctgtca tcttctcctc
    16501 cttaagacag agtcgctcca aaaatagtcc gttgcttcaa aagtcacagc tatgtctaat
    16561 aataaaagag gtggtttatt ctctaaaagt tatacaagaa tgatggcgat ggagccgcat
    16621 tgcagcatcc tagcctcgca tcatataatg aagagccaaa ttattgggac gggaggcggt
    16681 ttgtgaaaat actgtttatt tcaagcgaat tgacaccgtt tgcaaaaagc ggcggacttg
    16741 gagatgcagt atcttctctt gcggctgcgc tttccaaatt gggtcacgat gtgcgagttc
    16801 tgcttccacg atactatttc atatccaaga atacattgcg aaggataaat ggtcttctag
    16861 agatcgattc tgcgcgagag cgccaccgag catttcttta tacggccaat ttgccaaact
    16921 cccaagctaa agtctatctt ctagaatgcg atagtctata tggtcgcaat ggaatatatg
    16981 gctattcctc aaaccaagaa tttcctgata atcccgagcg attttctgtt ttaagcaagg
    17041 ctgccttcgc actctgccga aacctcggat ggattccaga tatcatgcaa gcgcatgatt
    17101 ggccaacggc accagtacct gtttacctta atactataga aaagaatacc gaattttcag
    17161 gaactgcggg agtcttgacc atccacaatc taggctatca gggaatctat agcagagacc
    17221 attttgccga gcttggttta gggtgggaat attattatgg agcggggttc gaatactatg
    17281 gcaacatcaa ttttctcaag gcagggatag tttctgccga atgcatagca accgtatcgc
    17341 ctacttacgc tatggaaatc caaacgccaa gcggcggttt tggactcgac ggtttgttac
    17401 gtcagcgaag cagagatctg gttggtattc taaatggtat cgatacagag gtatggaatc
    17461 ctttgacaga tcccttttta ccagcacatt attcagccgg agatatcagt ggaaaagcta
    17521 tctgcaaaga agctcttcaa aaagagttag gtcttcctat tgatccgcaa gcaccgctga
    17581 taggcatggt aacgagactt gtggagcaaa aagggatttc tgaggtattt ggtcgcacat
    17641 acggatgcat gcgtaaaatt ctggagacta ttcatgttca ggtcgctgtg ctcggctccg
    17701 gagatgcttg ggcagaagag gcaattatga atttttcagc ccagtatccg caattcaagg
    17761 gagtcattgg atacaatgaa aggcttgcac atctcatcga ggcggggagc gactttttcc
    17821 tcatgccaag cagatatgaa ccctgtgggc taaaccagat gtattcgctc gtatatggga
    17881 ccttgccgat tgtccatagg acaggcggtc tagctgacac cgtggtcaat tataatcagg
    17941 acttgggcga tggcacaggt ttcatgttcg atgatttgac tccacaagcg gtatacgata
    18001 cggtgggctg ggcaatgtgg gcgtggtata acagacctga gcatataaaa actatgcgcg
    18061 agcgagcgat gaaacaagat ttctcatgga atcgctcagc tgaagagtat ggacggcttt
    18121 atcagcatgc tttagcccta agaaaggcta agtgaatctt aaaaagaagt ctatacaaat
    18181 aagataaaag gaacacatcg atgtctgagt ctaaaatact ggattacaat aagcaggagg
    18241 taagagatca gcttttgcat gccttgcgca gctatacaaa ggaatggact agcgcagatc
    18301 ttgctcgagc aacaggctta cccctagcac agattaatgc tgaaatgccc actatctccg
    18361 atgaatatcg aggcagaatt cgcgtatcgg agagaggtga tctcttgtat tcctttccta
    18421 atggattcaa aagcaaatac aagggttttg gaccttctat gcgaagattg tggaagacca
    18481 tcactaaagg agcggtagaa acaggaaaat tcctttttaa ggtgtggatt ctagttactc
    18541 tctttggtta tttctttatt tttttagccc ttgctctagt tgctctcttt ggctctgtcg
    18601 cgatgcgcgg aggtggatct cgtgatagcg atgaccgccg aggcggtggc ggacttggtg
    18661 gct
//
LOCUS       DAOSOB010000036        18263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3062_length_18263_cov_64.883513, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000036 DAOSOB010000000
VERSION     DAOSOB010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 18263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18263
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3062_length_18263_cov_64.883513"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="PLS88_05230"
     CDS             <1..1040
                     /locus_tag="PLS88_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU06778.1"
                     /translation="LSIGTLRIDEQIRESAQQPRQFIEMYLESNIKTACDILAEDLQL
                     DSVRTFVLIGADARFAAHCLSDKVFNNYTVLDRKQFIEFADYVASMPTEECMAQYHLS
                     WNEAESYAAGLTIERMFLERTGADTIIVPNISIREGILLAQVQGMDKKIEEELRRQVV
                     ASARALGKRFHYDEAHAAHVRNLSLKIFDALKKEHGLGIHERMLLEISAILHDIGTYI
                     RTSGHHKHSEYIVANSEVFGLNRDDINIVSNVVRYHRKSQPLSTHVNFIALPREGRIV
                     VLKLAAILRVADALDRSHQGRIKELSFEQTEDRFIIRTSESLDLSLEKLSLAEKGDMF
                     EDVFGLMPVLI"
     gene            1072..3234
                     /gene="ppk1"
                     /locus_tag="PLS88_05235"
     CDS             1072..3234
                     /gene="ppk1"
                     /locus_tag="PLS88_05235"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOU06779.1"
                     /translation="MKEFPMLNRELSWLDYNARVLEEGLKPSVPLLEKLNFMCIFASN
                     LDEFFMVRVAAVKTAALTGQINEEWAGMEPHALLEAIHEKVQACYDKLYVHLHGTILP
                     SLAEYGIQLVQEKDWTTKESRYLERLFAEKIYPLLTPLRLEDNHFPSTGSLQVHVAFR
                     LEENDSVAIIQVPKNIDRFIVLPQYLSLESDNSGSSATGKAFRIALLEDVILAFGSKL
                     FAGLRVSSGLVFKVNRDADFSVDEDRDDDFLTAMEEVLTGRQNSTPVRMVYSGEDQVL
                     INTLMQSLSLDALDLYQADWLIDLGRANELCDSAFYKVHGFTPDRALFFRPWKPIRIF
                     EEKSSIFDWIDEHDRFVNLPYESFDVVQRFIEESASDPSVLGIKVTLYRTSGLNSPFV
                     HALAKAARSGKQVVVVLELKARFDEEKNISWATELEQAGAIVTYGVARFKVHAKAALI
                     IRKRQDGSIARYIHLSTGNYNEKTARSYVDFCLFTSNPSLCTDIALFFNILTGYSSIR
                     SLSLIALSPFDLKDRIIGFIEREIAQATPESPGLIIAKLNSLSDPDLIAALYRASAKG
                     VQIKLNVRGVCTLIPGVAGLSENIEVCSVLGRYLEHGRMLYFRNGGSEELYISSADWL
                     QRNMKKRLELLFPVLDKEIKARCKDILNIYFADNTHAYRLKNDGTWIDLSLSRRPEDP
                     LLYAQEKLYKMARKTARNLEEKQEILQVRRSKGKSIGQ"
     gene            3288..4082
                     /locus_tag="PLS88_05240"
     CDS             3288..4082
                     /locus_tag="PLS88_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOU06780.1"
                     /translation="MKAEKETLDHIRNKKAFIIDMDGVIYHGNVLLPGADLFVEWLKY
                     EKKRFLFLTNSSERSPEELSQKMARLGIEVGSEHFYTSALATAAFLASQKPNGTAYVI
                     GEPGLIHALYRIGYTMNNVNPDYVVVGEASSYNLDTLIKAVRLVLGGAKLIGTNPDLT
                     GPTEGGLVPACGALVAPIELATGRKAYFVGKPNPLMMRHALRTLGTTHEETIIIGDRM
                     DTDIIAGIESQIETILVLTGVTEEKDMPKFAYAPTYVLESVSEIPQ"
     gene            complement(4098..5525)
                     /locus_tag="PLS88_05245"
     CDS             complement(4098..5525)
                     /locus_tag="PLS88_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU06781.1"
                     /translation="MIQESSYIYQIFLRNFTQEGTFRAAIPELERISNLGFDWIYLTP
                     IHPIGGKSRKGTYGSPYAIRDYRKINPELGDETDFTNFVEEVHAHDLKLMIDIVFNHT
                     SPDSVLVHEHPDWFIRAADGLPWRKCEDWSDVVDFDYRSSPHLWLELIDTLAMWRDRG
                     VDGFRCDVASLVPSEFWRQARQRVNKYDPGIKEEKAPLVWLAESVHPAFLRKMRLLGY
                     NSWSEPELHAAAFDLTYDYDGWERLEKIWEQGMSFERYLEYLYVQETLYPKGAKKLRF
                     LENHDQERAAHRFGNRAKLRAMTPFYQFLPGVSMSYMGQELALEHKPNIFEKDPIELE
                     LGDKEFERYFIASLRATKEAKEEAPFFSWTIGQDFRLKDGAAREEVIYCLRSVEPTKM
                     PYPIDRSMIRKGNYLLVIQPELDSPARQRFYGSASKKASSSLEYTPSALFNIEGTDLL
                     SGEEVRIAEGDKIPRIPASLIRLED"
     gene            5623..6459
                     /locus_tag="PLS88_05250"
     CDS             5623..6459
                     /locus_tag="PLS88_05250"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HOU06782.1"
                     /translation="MNKHLSRKHADRPNSVGYAVPVRAAPKSNRARGSNRASTMEKKE
                     NTEAFDDAFIAAARDYRMLLDKDYPVDASLKLVGDRYRLSHNGRMMLYRGILSLKASQ
                     INLAKLIRSPWERASLGVDGYNILFTLMNYMHGHPMFIATDGFLRDVGGAHGRIADHR
                     GFERMAEIAANYLAALNLESLTFFLDAPVSSSGRQAGFLREAFAQRSIDAEVRLENGV
                     DHFLAYWEGELIATADSAIIGRTNAHVFDLARAILENQFDARFMSLARLLEPSLSISV
                     PP"
     gene            complement(6443..7108)
                     /locus_tag="PLS88_05255"
     CDS             complement(6443..7108)
                     /locus_tag="PLS88_05255"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HOU06783.1"
                     /translation="MSEILQLLIRPLGETILMIAASTLIATILGGALGILLFALSQDR
                     FAGKYPGALLLKRIADRVINMLRSFPYVILMVLVLPLSRLIVGTSLGTIASIVPLSIA
                     AIPFLARIVESSLSEVDSGVIDAAISCAASKARILFAILIPEALPSLISGLTLTMISL
                     LGYSAMAGVIGGGGLGDLAIRYGYQRFRADVMAIAVVAIIALVELIQWAGSRITSRIK
                     AAR"
     gene            complement(7101..8003)
                     /locus_tag="PLS88_05260"
     CDS             complement(7101..8003)
                     /locus_tag="PLS88_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU06784.1"
                     /translation="MFHAKRPEEAADQALVAHPSHHTPHHGASFTPIGAFPKDIGDSH
                     TKKIELAIRIDRVSKSFGNHSVLNEISLDVPKGNIHGIMGKSGAGKTTLIRIAGLLEK
                     PDEGSIWYEGISMPVHELYGRAMLEARRQVGFVFQSFNLFSSRTAAGNIAFPLEAAGW
                     PREKIRVRVEELLELVELSDKADRPACRLSGGEKQRVAIARALANSPSMLLSDEATSA
                     LDPETTRSVLDLIRSLRDRLGLTVIMVTHQKEVIRRICDTVSVISGGSIVEQGSVAEV
                     FSNPISEAAQAFLKEDDEKEYGYV"
     gene            complement(8250..8666)
                     /locus_tag="PLS88_05265"
     CDS             complement(8250..8666)
                     /locus_tag="PLS88_05265"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOU06785.1"
                     /translation="MREKATVKKIKGDMATVSIEMQEGCSLCVNNSRCKIRKSDLAVY
                     NKGHIDIDEGDCVVIEMPTSEQAKSAFWVLGFPLIMLFVGYGFGALAFGGSGEGPAVA
                     SAGAGFVLALGIGTLIQRRHKLESFPYILGKEGEVF"
     gene            complement(8629..9462)
                     /locus_tag="PLS88_05270"
     CDS             complement(8629..9462)
                     /locus_tag="PLS88_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOU06786.1"
                     /translation="MGLTIVFLGEIVGKTGVFTVKSTMPEIRQRFEPDFIFANANSAT
                     GGAGLGIQHAVYLRKLGIDCITMGEAAYYKPDMTEFFPKAGWVLRPANLPEGDPGRSW
                     RVFEKNGKKVAVVMLLGQSGFVRIHADNPFLAIDRLSNFLHRETAYIIVNFHAATTAE
                     KLSMARYTNGKVSAILGSGGKVLTADARILSQKTAAITDLGRTGSMLSVGGFDPEAKV
                     KEFLTGIPTWCREASAQPEAQGTCIHFDDDGSAISIEPFRICGKEVVDEGESDSKKNQ
                     G"
     gene            complement(9550..10035)
                     /locus_tag="PLS88_05275"
     CDS             complement(9550..10035)
                     /locus_tag="PLS88_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOU06787.1"
                     /translation="MSEYRRPTWDEYFMEVANAIAKRATCDRGRSGCVIAKNNQILAT
                     GYVGAPSGLPHCDDIGHQMRKMLHEDGSITEHCVRTVHAEQNAICQAAKKGVAIEGGT
                     LYCRMTPCRTCAMLIINCGIVRVVAEYRYHDAEESEAMFKMARVDLEYVHEEILQYSR
                     Q"
     gene            10251..11165
                     /locus_tag="PLS88_05280"
     CDS             10251..11165
                     /locus_tag="PLS88_05280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU06788.1"
                     /translation="MKTNKGTIVINLEFEKAPMTVGNFVGLAEGSLDATKGKHFYDGL
                     TFHRVVANFVVQGGDPIGDGTGGPGYRFPDEIVPELKHDGPGVVSMANAGPNTNGSQF
                     FITIEAAPWLDGKHSVFGKVIEGMDVVRQIQQGDRIESITIQRVGAKAKSFDSSQQAW
                     DQRLSAAYATLNALNKQKRDSDLELIAQKWPDLNTDDNGIFQKIIHKGFGSMPTPGSK
                     VSVIYKGMLLDGTVFDQSALSGSPFTFTIGQGEVIDGWDLVVSTMKKGEKRMIIIPPE
                     LAYGSQSIGDVIPPNSFLVFEIELVSIE"
     gene            11408..11968
                     /locus_tag="PLS88_05285"
     CDS             11408..11968
                     /locus_tag="PLS88_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06789.1"
                     /translation="MRRGIKCISVRNTMLVIAATILLVGIPFVNAQTSGKTSVSIQIM
                     VYVPSILNLTLDFSSSGIAQLVAYLGDSRDVRGKGFELKSNSVVPLGAAHVISNLSSG
                     YSIVVQSMNGCALKNQDSEGTVNYDLILGGIPAMRQGDSFKLNCCGTTPFEGRVLPVS
                     IALGNVPSNAAPGLYADNLLFNIMAN"
     gene            12095..12511
                     /locus_tag="PLS88_05290"
     CDS             12095..12511
                     /locus_tag="PLS88_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOU06790.1"
                     /translation="MTKEIQMSWIEDMAFESMLDGHRLVLDADDISGGKDRGPRPKGL
                     ILAALGGCSGMDVISILRKMREPITWFNIKASGNVAEEHPKKFTSFKLIYQFKKSDKL
                     NPENVLKAIELSQTKYCGVAATLKDGHEITWEIEYL"
     gene            12718..14805
                     /gene="fusA"
                     /locus_tag="PLS88_05295"
     CDS             12718..14805
                     /gene="fusA"
                     /locus_tag="PLS88_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU06791.1"
                     /translation="MAFDVHKMRNIGISAHIDSGKTTLSERILFYCQKIHAIHEIKGK
                     DGAGAAMDSMELERERGITIQSGATNVQWKDHSINLIDTPGHVDFTIEVERSLRVLDG
                     AILVLCSVAGVQSQSITVDRQLKRYHVPRLAFINKCDRMGANPSKVKVQLAEKLGLNP
                     TFITIPIGLEDKFEGVIDLIDMRAIYFDGPNGEELRYAEIPIHLRDDSERYREELLEA
                     ASMFSDELAEAYLEGNPTKELLIKAIRKGTIEEKLVPVFVGSAYRNKGVQPLLDGVIN
                     FLPDPSERQNIALDLSNEEAEVMLQANDQAKTVALAFKLEEGQYGQLTYVRIYQGMIR
                     KGDELYNSRARKRFRVGRLVRMHSSSMEDIQEGNCGDIVALFGIECASGDTFCHPDLN
                     YTMTSMYVPEPVISLAITPKDNKSSDQMSKALNRFTKEDPTFRTYVDPESNQTIVQGM
                     GELHLEVYIERMKREYKCEVIPGMPQVAYRETITQRADFNYTHKKQTGGSGQYGRIAG
                     YIEPFKEGSYEFVDLIKGGAIPNEFIPSCDKGFRAAMEKGTLIGFPVTGVRCVINDGQ
                     SHPVDSSDIAFQFAAIGAFREAYEKAKPAILEPIMKVVVETPNEFQGNVFASLNQRRG
                     IIMANTEDNTFSRIEAEVPLSEMFGYSTVLRSLTQGKAEFSMEFEKYARVPAALAEQL
                     RKDYLEKRKKEQK"
     gene            14867..15571
                     /locus_tag="PLS88_05300"
     CDS             14867..15571
                     /locus_tag="PLS88_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOU06792.1"
                     /translation="MLKEEFIQKSPVRIFEKSIDGGLHAGNIGILASKKGIGKTSVLV
                     QIALDKLMQGEKVIHVSFNAHTSYIISWYENIFNEIARRKNLENIDEIKEQVIRNRMI
                     MNFMQESISVDQIMRTIKALISDGSFNAKTLIIDGFEFTRATSERFEKVKAFLSEMQL
                     EGWYTCTLAETDPVLDSKGIPIVLRDIEPYISVIVLLEPKKDYIHLRVVKNHDRLEQG
                     DMPLMLDPRTLLIAEA"
     gene            complement(15572..16798)
                     /locus_tag="PLS88_05305"
     CDS             complement(15572..16798)
                     /locus_tag="PLS88_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOU06793.1"
                     /translation="MSKLREINLAIRPAAMHTLFMGSSMRAIAACPIGLEKVLAHDLA
                     HLGYHDVGRSAGRVFFNVREQSLTEDLARANIGLRTADRVLLVAGEFKAFDFDGFYQG
                     VFSIPWECFCTKETKVVIERVRLQYCVLHSQATLQSMAQKSIYASLMERFHIRTMPET
                     GNTLEVRIYGDNNWWQIVVDTSGEALSKRGYRRLTHMAPLKETIAASMLFLAGWSRAR
                     PLIDPFCGSGTIAIEAALYACNVAPGLRRRFAFEFFPSIDSNKIEDVRIAFRGQARKD
                     IRADIRASDIDKDALALARKNASIAGISEFVRFAQVDARNIVPLGSRGVLLANPPYGQ
                     RMGSADKALSLYKELGPILERFLEAGWDCGFLSSISSFGDAVGITPVSVREIVSGQEK
                     IYFNWFSGTEGLKAQS"
     gene            16892..16965
                     /locus_tag="PLS88_05310"
     tRNA            16892..16965
                     /locus_tag="PLS88_05310"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16926..16928,aa:Ala,seq:ggc)
     gene            17585..>18263
                     /locus_tag="PLS88_05315"
     CDS             17585..>18263
                     /locus_tag="PLS88_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU06794.1"
                     /translation="MKRFLLMALAVLMMASTSLSAAPAKIEAELNLITPVASFVSVAA
                     LDAFKVWAKANYGIDVKTSYTAKGTQLAAGLIREWGSNPQADVIWGGETVLYDMMAAD
                     GFLIKHEAPKALLDRIPATMGAPKPMPLKDPKGFWTGTALEPYGIVYNEGLVTRRLRT
                     TPPKTWEDMLNNPKFKGQIAQCTPDNSSSSHATYEVILQKYGWEKGWEWLSKLGAYTG
                     QFVAKSGD"
BASE COUNT         5152 a         4269 c         4096 g         4746 t
ORIGIN      
        1 gccttagcat aggcactctt agaatagatg aacagatccg cgaatcagca cagcagccca
       61 ggcaattcat agaaatgtat ctcgaaagca atataaagac agcatgcgat atcttggccg
      121 aggatttgca gctcgattct gtgcgcacat tcgttcttat tggtgcagat gcgcgctttg
      181 ctgcacactg cctttcagat aaggtcttta acaactacac agtactagat cgcaagcaat
      241 tcatcgagtt tgcagactat gttgcaagta tgcctaccga ggagtgcatg gctcaatatc
      301 acctttcttg gaatgaagcc gaaagttacg ccgcggggct cactatcgag cgcatgtttc
      361 ttgaaagaac aggagcagat accattattg tgcctaatat atccattcgc gaaggcattt
      421 tgcttgcaca ggtacagggc atggataaga aaatcgaaga ggaactacgc agacaggtag
      481 tagcatcagc ccgtgctctt ggtaaacggt tccattatga tgaagcccat gccgctcatg
      541 tgaggaacct tagccttaaa atattcgatg ccctaaaaaa ggaacatggc cttggcatcc
      601 atgagagaat gctactcgaa atctcagcaa ttcttcatga tatagggact tacataagaa
      661 caagtggaca tcataaacac agcgagtata tcgtcgccaa ttcggaggta tttggtctca
      721 acagagatga tatcaacata gtatctaatg tagtgcgcta ccaccgaaag tcccaaccgc
      781 tttctaccca tgtcaatttc attgcccttc cgcgagaagg tcgcatcgtt gtactcaaat
      841 tggcggccat tctgcgcgtt gccgatgcgc tagatcgaag ccatcaaggc cgtatcaagg
      901 agctctcttt tgaacagacc gaggatagat tcataattag gacttcagag tcactggatt
      961 tatcgttgga aaagctctct ctagcagaga aaggtgatat gttcgaagat gtctttggac
     1021 tcatgccagt tttaatctag aacatatcaa tgtaagcaaa aagagaagag tatgaaagaa
     1081 tttccgatgc tgaaccgtga gctttcttgg ctggattaca atgcacgcgt acttgaagaa
     1141 ggactaaagc cttctgtgcc tctgctcgaa aagctcaatt ttatgtgcat ttttgcatcg
     1201 aatctggatg aattcttcat ggtgcgtgtc gcggccgtca agacagctgc actgacagga
     1261 cagatcaacg aagaatgggc tggtatggag ccacatgctc ttctagaggc tattcatgag
     1321 aaagttcaag cttgctatga caagctatat gtccatctgc atggaacgat tctccctagt
     1381 ttggccgaat atggtattca acttgttcag gaaaaagact ggacgaccaa agaaagcagg
     1441 tatctcgaaa gactttttgc agaaaagata tatcctctcc ttaccccatt acggctagaa
     1501 gataaccatt tcccatcgac aggaagtctt caggtccatg tcgcatttag actcgaagag
     1561 aatgactcag tggcaatcat tcaagtacct aagaatatcg atcgttttat tgttctgcca
     1621 caatatctaa gtctggagtc cgataattcc ggaagttctg ctactggtaa agcctttcgc
     1681 atcgctcttc tagaggatgt cattctggca tttggaagta aactctttgc cggtttaaga
     1741 gtatcgagcg gccttgtatt caaggtaaac agagatgcag atttcagcgt ggatgaagat
     1801 agggacgatg atttcttgac cgcgatggag gaagtactca cagggcgaca gaattctacg
     1861 cctgtgcgca tggtttattc gggagaagat caagttctta tcaacaccct gatgcaaagc
     1921 ctctcgcttg atgccctgga tctatatcaa gccgattggc ttatagacct gggaagagca
     1981 aatgagctct gcgattcagc gttctataag gttcatggct ttacgccgga tagggcgcta
     2041 ttcttccgac catggaaacc tatccgcata ttcgaagaaa agagcagcat tttcgactgg
     2101 atcgatgaac atgaccgctt tgtaaatttg ccttatgaat ctttcgatgt tgtccagcgc
     2161 ttcatagagg aaagtgcgag cgatccatcg gtcttaggaa taaaagtaac actctataga
     2221 acgagcggcc tgaattcgcc ttttgtgcat gccctagcta aagcggcacg gagcggaaaa
     2281 caggttgttg tcgtcctcga gctcaaggca cgcttcgacg aagaaaagaa catctcatgg
     2341 gctaccgagc ttgaacaggc aggtgcaatc gtaacttatg gcgttgctcg gtttaaggta
     2401 catgcaaagg cagccttgat tattagaaaa cgacaagatg gtagcattgc ccgatatatt
     2461 cacctgtcaa caggaaatta taacgaaaag accgcaagaa gctatgttga cttttgtcta
     2521 ttcacctcaa atcctagtct atgcacggat attgctctct tcttcaacat tctcactggt
     2581 tattcgagca tccgatcgct ctcactgata gctcttagcc cttttgatct caaggatcgc
     2641 atcatcgggt ttattgaaag agagatagca caagccactc cggaatcacc aggcctcata
     2701 atcgcaaagc tcaattcctt atcagatcca gacttaatag cagctctcta tcgcgcttct
     2761 gctaaaggag tacagataaa gctcaatgtg aggggagtct gtacactgat cccaggggta
     2821 gctggccttt cagaaaatat cgaggtctgc tcggtcttag gacgctacct tgagcatggt
     2881 cgaatgctat acttcagaaa tggtggctcg gaagagctat atatctccag cgccgactgg
     2941 ctccagcgaa acatgaaaaa aaggctggaa ttgctatttc cagtgctcga taaagagatc
     3001 aaagcaagat gcaaggacat tctgaatatc tactttgcag acaatactca tgcctatagg
     3061 cttaaaaatg atggcacatg gatagacctc tcgctctctc gacgtccaga ggatcccctt
     3121 ttatatgcac aagaaaagct ctataaaatg gccagaaaaa cagctcgaaa ccttgaggaa
     3181 aagcaggaaa tccttcaggt aaggcgatcg aaagggaagt ctattggcca ataaactcta
     3241 taatgctttc tataaaagat aaaatttaat ttgcaaggac aagagacatg aaagcagaaa
     3301 aagagacttt ggatcatatc cgaaataaga aggcattcat aatcgacatg gatggcgtga
     3361 tttaccatgg gaatgtactt ttgcctggcg ccgacctatt tgtagaatgg ctcaaatatg
     3421 agaaaaagcg atttttattc ttgactaatt cgagtgaacg ttctcctgag gagctttcgc
     3481 aaaaaatggc aaggcttgga atagaagtcg gatctgagca tttctataca agtgctcttg
     3541 ctacggctgc cttcctagca agccaaaagc ctaatggcac agcttacgtc attggagagc
     3601 caggactaat ccatgcattg tatcggattg gttatacgat gaacaatgta aaccccgatt
     3661 atgtcgttgt cggcgaagct agcagttaca atctcgacac tctcataaag gctgtgcggc
     3721 tcgttcttgg aggagcgaaa cttataggca cgaacccaga ccttacaggc cctaccgaag
     3781 ggggcctagt acctgcctgt ggggcattgg tcgcaccaat cgagcttgcc acaggccgca
     3841 aggcatactt tgtcggcaaa cccaatccac tcatgatgcg acatgcgctc cgaactctag
     3901 gaacaacaca tgaagagaca attatcatcg gcgacagaat ggatacagac ataatagcag
     3961 gcatagagtc tcaaatcgaa accatactcg ttttgacagg tgttaccgag gagaaagaca
     4021 tgccaaaatt tgcctatgca cccacttatg tgctcgaaag tgtttcggaa ataccacaat
     4081 aaggcagatt tggtccgtca gtcttctaag cgaataaggc tagcaggtat tctcggtatt
     4141 ttatcaccct ctgcgattcg tacttcttct cccgaaagca aatctgttcc ttcgatgtta
     4201 aagagagcgc taggtgtata ttccagcgag gaagaagctt ttttggatgc acttccataa
     4261 aatctctgcc tagcaggact atcaagttca ggctgaatta caagaagata atttcccttc
     4321 cttatcatag atcgatcaat agggtaaggc atttttgtag gctcgacact ccttaggcaa
     4381 tagatgactt cttctctggc agccccatcc ttcagtcgga agtcctggcc aattgtccat
     4441 gaaaagaaag gagcctcctc ttttgcttct tttgtagccc gcaagcttgc aataaaatag
     4501 cgctcgaact ccttatcacc tagctctagc tctatcggat ccttctcgaa aatgttgggt
     4561 ttatgttcga gagccaattc ctgccccatg tagctcatcg atactccagg caagaattga
     4621 taaaaaggag tcattgcgcg cagcttcgct cgatttccaa atctatgagc cgcgcgctcc
     4681 tgatcatggt tttcaaggaa cctgagcttt tttgctcctt ttggatagag tgtctcctga
     4741 acatagagat attctagata tcgctcaaat gacattcctt gctcccatat tttctcgagc
     4801 ctttcccaac catcataatc ataggtcaga tcgaaggcag ccgcatgcaa ctcaggctcg
     4861 ctccatgaat tgtacccaag caatctcatc ttccttaaga aggccggatg aacgctctcc
     4921 gcaagccata caagcggagc cttctcctct tttataccag gatcgtattt atttactcgt
     4981 tgccgagcct gtctccaaaa ctcacttgga acaagagatg ctacatcaca acgaaaaccg
     5041 tccactcctc tatctctcca cattgctagc gtatcgatga gttcaagcca gagatgaggc
     5101 gaactcctat aatcgaaatc gaccacatcg ctccaatctt cgcactttct ccatggaagc
     5161 ccatcggccg ccctgataaa ccagtctgga tgctcgtgca caagcactga atcaggtgaa
     5221 gtatgattga atactatatc tatcatgagc ttgagatcat gagcatgtac ttcttccaca
     5281 aagttcgtaa aatcagtctc gtcccccagt tcaggattga tcttgcgata gtctcttatg
     5341 gcatagggac tgccataagt acccttacga gactttcccc ctatagggtg tatcggtgtg
     5401 agatatatcc agtcaaaacc caaattagag atgcgctcga gctctggtat agctgcgcga
     5461 aaagttccct cttgagtgaa gttgcgcaaa aatatttgat agatataact gctttcctga
     5521 atcatcgacg acttcctctt attcttctaa taggcttgag aatacataat aaacaatatc
     5581 agagagccat agaaccagca agcaagacga aagatcatca gcatgaataa gcatttgtcg
     5641 agaaagcatg ctgacaggcc aaatagtgta ggatatgctg taccagtcag agctgctcct
     5701 aaatcgaata gagcaagggg ttccaatagg gcaagtacta tggaaaagaa agagaatacc
     5761 gaagcctttg acgatgcatt tattgctgca gcaagggatt atcgaatgtt actcgataag
     5821 gactatcccg tcgatgcaag cctcaagcta gtaggcgatc gctataggct ttctcataac
     5881 gggcgcatga tgctatatag agggattctt tctctaaaag cttcccagat caatcttgca
     5941 aagcttattc gtagcccatg ggagagggct tcgctcggtg tcgatggcta caatatcctt
     6001 tttactctta tgaactatat gcatggtcac cctatgttca tcgctacgga tggatttctg
     6061 cgcgatgtag gtggcgcaca tgggcgcatc gcggatcacc gaggattcga gcgcatggct
     6121 gaaatcgcgg caaattacct agctgctcta aatctcgaaa gcctaacttt cttcctcgat
     6181 gcgccagtat cttcgagcgg ccgacaagct ggatttctgc gagaagcctt tgcacagcga
     6241 tctatcgatg ccgaagtccg ccttgaaaat ggggtcgatc attttctagc atattgggaa
     6301 ggcgaactta tagctacggc agactcggct ataataggac ggaccaatgc ccatgtcttc
     6361 gaccttgccc gcgcaatact cgaaaaccag ttcgatgctc gctttatgtc ccttgcgcgc
     6421 ctcttggagc ctagtctttc tatcagcgtg ccgccttgat tcggcttgta atcctcgaac
     6481 cagcccattg aatgagctca actaatgcaa tgatagctac tactgctata gccattacat
     6541 ctgcgcggaa gcgctgatag ccatatcgaa tcgccaaatc tccgagtccg cccccgccaa
     6601 ttactcccgc catggctgaa taaccgagca agctgatcat agtcaatgtt aagccagata
     6661 taagcgacgg cagtgcctca ggaattaaaa tcgcaaagag aatacgcgcc tttgaggctg
     6721 cacaggagat tgctgcatcg attacacccg aatctacttc ggagagcgat gattccacta
     6781 tgcgcgccag aaaaggaatt gcagctatcg ataaaggcac aatggatgcg atggtaccaa
     6841 ggctcgttcc gactattagt cgggatagag gcaatacaag caccatcagg ataacatagg
     6901 ggaaagaacg aagcatattg attacgcgat ccgctatgcg cttgagcaaa agagctccag
     6961 gatacttgcc tgcaaagcga tcttgcgaaa gagcgaatag caatattccc aaagcgccac
     7021 caagaatggt cgctatcaat gtcgaagccg cgatcatcag aatggtctct ccaagaggtc
     7081 gaataaggag ttgcagtatt tcagacatag ccgtactcct tttcatcgtc ctccttgaga
     7141 aaagcttgtg ctgcttcaga aataggattg gagaagacct ccgcaacaga gccttgttcg
     7201 acgatagagc caccagagat caccgataca gtatcgcaga tgcgccgtat gacctctttc
     7261 tggtgagtca ccataatgac ggtaagacct agtctgtctc tcagagatcg aatcaaatcg
     7321 agcactgatc ttgtcgtctc tgggtcgagc gcactcgttg cttcatcgct caagagcatc
     7381 gatgggctat ttgctaatgc gcgggctatt gccactctct gcttttcgcc gccggaaaga
     7441 cggcatgcag gcctatctgc cttgtccgaa agctcaacaa gctcaagaag ctcttctacg
     7501 cgcacacgaa ttttttctcg aggccaacct gcagcttcga gtggaaaggc aatattgcca
     7561 gcagcggtcc gcgaagaaaa tagattaaaa ctttgaaaca caaagccaac ctgcctacgg
     7621 gcttcaagca ttgctcgccc atacaattca tgcacgggca ttgagatgcc ttcgtaccag
     7681 atcgagcctt cgtctggctt ctccaaaaga cccgcaatac gtatcaaggt agtcttgcct
     7741 gctccgctct tgcccataat gccatggatg ttccccttgg gcacatctag cgatatctca
     7801 tttagcacgc tatggttgcc aaaagacttt gacaccctat cgatgcggat cgccagctcg
     7861 attttctttg tatgagaatc gccaatatct ttgggaaaag cccctatagg agtaaaggac
     7921 gccccgtgat gcggggtatg gtgggaagga tgcgctacta gcgcctgatc agcggcctcc
     7981 tctggccgct ttgcatgaaa catgaaagat cttgtgtctg aatcataggt tttcctttaa
     8041 gacagaacca tagaagattt tgtctctctt tgtcaatatg caaaagcaat ggagcagagt
     8101 tagctttgta aatatttcgt gcggacttct taatctgcac ttcttaatgc gcacttctta
     8161 atctgcattt gttcgtccac actcgacagt ggaagactaa aaagcagatt tgccaatcgg
     8221 actggtttat cgcctaacta tcgcttctct taaaaaacct cgccttcctt tcctaaaata
     8281 taaggaaaag attctagctt atggcgacgc tgaataagcg ttcctatgcc aagagcaaga
     8341 acaaaacctg caccagcact cgccactgca ggtccttcgc ccgatccccc aaaggcgaga
     8401 gctccaaagc cataacccac aaaaagcatt ataagaggaa aacctaagac ccaaaaagcg
     8461 ctctttgctt gttcacttgt gggcatctcg attactacac aatctccttc atctatatcg
     8521 atatggcctt tgttatacac ggcaagatcg cttttacgta ttttgcacct gctattgttg
     8581 acacacaaac tgcaaccttc ttgcatctca atcgaaacgg ttgccatatc acccttgatt
     8641 ttttttactg tcgctttctc cctcatctac gacctccttg ccgcaaatgc ggaatggttc
     8701 aatcgaaatc gcactgccat catcatcgaa gtggatacag gtgccttgtg cttctggctg
     8761 agcgctagcc tctctgcacc atgttggtat gccagtcaag aattctttga ccttggcctc
     8821 tggatcaaac ccacctaccg acagcatgct gcccgttctg cccagatcgg tgatagccgc
     8881 tgtcttttgg ctgagaattc gagcatcggc tgtgagcacc ttgccccctg aaccaagtat
     8941 ggctgaaact ttgccattcg tgtaccgggc catagagagc ttctcggcgg ttgtcgcagc
     9001 gtgaaaattg actatgatat aggcggtttc cctgtgtaag aaattgctta gccgatctat
     9061 agccaaaaag ggattatccg catggatcct cacaaatccc gactgcccta gaagcattac
     9121 taccgcaact tttttgccat tcttctcgaa gactctccaa ctgcgcccgg gatctccctc
     9181 gggaagattg gccggcctca gaacccagcc cgctttagga aagaattcag tcatgtctgg
     9241 cttataatag gctgcttcac ccatcgtaat acaatcgatc cctaatttgc gaagataaac
     9301 tgcgtgttga atgcctaggc ccgcccctcc cgttgcagag tttgcatttg caaaaataaa
     9361 atcgggctcg aagcgctgcc gaatttcggg cattgtgctt tttactgtga atacgccggt
     9421 ctttccgacg atctcaccaa gaaatacgat cgttaatccc acaacctgct atgctagcgg
     9481 gaacttggct tttttacaac gggcttttat ggatatttaa gataataagg ctcttaagga
     9541 aagatctatt cattgtctcg agtattggag aatttcctca tgaacatatt cgagatctac
     9601 tctggccatt ttgaacattg cctcgctttc ctctgcatca tgatagcgat attcggccac
     9661 aacccgaaca atgccacagt ttatgatgag catcgcacag gtccgacatg gagtcatacg
     9721 gcaatagagt gtccctccct cgatggctac accctttttt gccgcttgac agatagcatt
     9781 ttgctctgca tgtacggtcc gaacgcaatg ttcagttata ctgccatcct cgtgcagcat
     9841 ctttctcatc tgatgaccaa tgtcatcgca atgtggcaga cctgaaggag cccccacata
     9901 tcctgtagct aaaatctggt tattctttgc aataacgcag cccgagcggc cacgatcgca
     9961 ggtagctcgt tttgcaatag cattagcgac ctccatgaaa tattcatccc atgttggacg
    10021 gcgatattcc gacatcagct tttcctcctt gccgacctct gcatttatac cgcataatat
    10081 cataggtaag attgtacatc gatgcgaata tacgcgaaac ccaaaaccaa ttaggagtcc
    10141 ttcagatgag gcagcgctct ataccattcg ttttcgtttt aatgatttta gcagttccta
    10201 tccaatcctg cagcgcacag acatctcgcc cagatggtat gtatgctatt atgaaaacta
    10261 acaaaggtac gatagtcata aacctcgaat tcgagaaagc acccatgacg gttggcaact
    10321 ttgtcgggct tgcagaagga tcccttgatg caaccaaagg caaacatttc tacgatggcc
    10381 tgacttttca tagagtcgtt gctaatttcg ttgtacaggg aggcgatcct attggagacg
    10441 gaacaggagg ccctgggtac cgattccctg atgaaatcgt accagaacta aaacatgatg
    10501 gaccaggagt tgtttccatg gctaatgcgg gccccaatac aaatggcagc cagttcttca
    10561 tcactattga ggcggctcca tggctcgatg gtaagcattc ggtctttggc aaagtcatag
    10621 aaggtatgga tgttgtaagg cagattcagc aaggggatcg catcgaatca attacaatcc
    10681 aaagagtagg cgccaaggct aaatccttcg acagttcgca gcaagcatgg gatcagcgcc
    10741 ttagcgcagc ctacgccact ctaaacgctc tcaacaagca aaagcgtgat tccgacctag
    10801 aactcatcgc tcagaaatgg ccagacctca atacagacga caatggcatc tttcaaaaaa
    10861 tcatacacaa gggcttcgga tctatgccta caccaggcag caaggtctcg gtgatctata
    10921 aaggcatgct cctagatggc actgtctttg atcaatcggc actttcgggc agtcctttta
    10981 catttaccat agggcaagga gaagtcatag atggctggga tcttgtagta agcactatga
    11041 aaaaaggaga aaaacgaatg ataataatac ccccagagct tgcctatggc tcacagagca
    11101 ttggtgatgt cataccgccc aactcctttt tagttttcga aatcgaacta gtctccatag
    11161 aatagcaagg ctcatgtcaa catcaaaggc ctataatgct ggaggctttg tggcttttgt
    11221 ctcattattg caaacaattt gactctttgg agataaaaga gatttgagcc gtgatttctg
    11281 tccgcttgga gataatggag tttattcgat taaatatctc caatagcaat gatgatttat
    11341 ccataaagcg atagatattt cagcagttcc tcaaacttaa ttcttcttct ttctctacaa
    11401 taaaattatg aggagaggaa ttaaatgtat ttctgtaaga aatacgatgc tggttatcgc
    11461 cgctacaata ctattggtgg gcattccgtt cgtgaatgca cagacatccg gcaagacaag
    11521 cgtttctata cagattatgg tctatgttcc ttcgatcctc aatctcactc tagactttag
    11581 ttcgagcggt atcgcacaac tcgttgctta tctaggtgat tcgcgagatg tgcgaggaaa
    11641 aggttttgag ctcaaatcta attctgtagt cccgcttggc gctgcacatg tcatttccaa
    11701 tctatcctca ggttactcaa tagtagtaca gtccatgaat ggctgcgcat tgaagaatca
    11761 ggattctgag ggcactgtca actacgatct tattctaggc ggcatcccag caatgcgcca
    11821 aggcgacagc ttcaagctaa actgctgtgg aacaacgcca ttcgaaggca gagtgttgcc
    11881 tgtaagtata gcattgggaa atgttccctc caatgctgct cctggtctat acgctgacaa
    11941 tcttctcttc aacattatgg ccaactgatt acagataata aagtcggttt aattttcttc
    12001 cttcaaaatc agcaaagaac aagctaaaag aaattataat ttcttcaatt ttttgataat
    12061 atctatcaag acaatccgta cagatggagg aatcatgacc aaagaaatcc agatgagttg
    12121 gatcgaagat atggcctttg agagcatgtt agatgggcat cgcttggttc ttgatgcaga
    12181 tgatattagt ggcggaaaag atcgtggtcc gcgtcctaag gggcttattc ttgctgccct
    12241 tggagggtgc agcggaatgg atgtcatatc gattctgaga aagatgagag aaccaataac
    12301 ctggttcaat atcaaagcaa gcggcaatgt agccgaagag caccccaaga agtttacaag
    12361 ctttaagctt atctatcagt ttaaaaagag cgataagctt aatccagaaa atgtactcaa
    12421 ggcgatcgaa ctttcacaga ctaaatactg tggagtagcc gcaaccttga aagatggtca
    12481 tgaaatcact tgggaaattg aatacctata gattggtgta taacagattt atgcaaccta
    12541 ttaacctgtc acttgttctt tttcatcatc ttttctataa ttgcgaaaag gtagcttctt
    12601 ttccagtatt tgttatactt tgcattattt tcagcaaatc agtcttgctg ccactaattt
    12661 gacactccct ttggctggtg taatcttaat attgtaggct aagaggtata gaaaaatatg
    12721 gcattcgatg tccataaaat gagaaatatt ggcatatcgg cgcatataga ttcgggaaag
    12781 acaactttat cagagcgtat tctattttat tgccaaaaga tacacgcgat acacgaaatt
    12841 aagggtaaag atggcgcagg cgccgcgatg gattcaatgg aattggaacg cgaacggggt
    12901 atcacaattc agtctggagc aaccaatgta caatggaagg accatagtat aaacctcatc
    12961 gataccccag gacatgtaga cttcaccatc gaggtggaaa gatctctacg tgtattggat
    13021 ggggccatcc ttgtgctctg ctcagtagca ggcgtccagt ctcaatctat cactgtagat
    13081 cgacaactta aacgctacca tgttccccgt ctagcattta tcaataaatg cgatcgcatg
    13141 ggcgcaaatc cttcaaaagt caaggtccag cttgcagaaa aactaggcct aaatcccacc
    13201 tttataacga ttcctatcgg cctagaagat aaattcgaag gcgttataga tttgatcgac
    13261 atgagggcaa tatattttga tggtcctaat ggcgaagaat tgcgctatgc agaaatccca
    13321 atacacctcc gagacgattc cgagagatat cgtgaagagc ttctggaggc tgcctcgatg
    13381 ttctcagatg agttagcaga agcataccta gagggcaatc cgacaaaaga gctacttatc
    13441 aaggctatca ggaaaggaac aattgaagaa aagctcgtgc ctgtttttgt tggttcggca
    13501 tatagaaata aaggcgtaca acccttgttg gacggggtaa taaatttttt gcctgatcct
    13561 tcggaacgac aaaatatagc cctcgatcta tccaatgagg aagcagaggt catgctccag
    13621 gcgaatgacc aagcaaaaac cgtcgctctt gcctttaagc tcgaagaagg ccaatatggc
    13681 cagctgacct atgtgcgcat atatcaaggc atgatccgca agggcgatga gctttacaat
    13741 tcacgcgctc gcaagcgatt tcgcgttgga aggcttgtga gaatgcattc ttcctcgatg
    13801 gaagacatcc aggaaggcaa ttgtggcgat atcgtggcgc tttttggcat cgaatgtgct
    13861 tctggcgaca ctttttgtca tcccgatcta aactatacaa tgaccagcat gtatgtaccc
    13921 gagccggtca tatcgctcgc cataacgccc aaagataaca agagttcaga ccagatgagc
    13981 aaagctctca atagattcac aaaggaagac ccaaccttcc gaacctatgt cgaccctgaa
    14041 tcgaatcaga caattgtcca aggcatggga gaactacatt tggaagtgta catagaacga
    14101 atgaagcgcg aatacaaatg cgaagtgatc cctggcatgc cacaggtagc ctatcgcgag
    14161 acaattaccc agcgcgctga tttcaactat acccataaga aacagactgg cggctcgggc
    14221 caatatggcc gtattgcggg gtatatcgag cctttcaaag aaggcagcta cgaatttgtc
    14281 gatttaataa agggaggtgc tattccgaat gaattcatcc cttcttgtga caaaggtttc
    14341 cgcgctgcta tggaaaaggg cacgttaatc ggctttcccg tgactggtgt gcgctgtgtg
    14401 atcaacgatg ggcaaagtca tcccgtcgat tcttccgata ttgccttcca attcgcggca
    14461 ataggtgcat ttcgtgaagc atacgagaaa gcaaaacccg caattttaga gcccataatg
    14521 aaggtcgtcg tcgaaacgcc aaatgaattc cagggaaatg tcttcgcttc gctcaatcag
    14581 cggcgtggaa tcattatggc caataccgaa gacaatacct tctctcgtat cgaagcagaa
    14641 gttccgctct ctgaaatgtt tggctattcc acggtgcttc gctctctaac tcaaggcaaa
    14701 gccgaatttt ccatggaatt cgagaagtat gctcgcgttc ctgctgctct tgcagaacaa
    14761 ttgcgcaagg attacctaga aaagcgcaaa aaagaacaga agtaaaatcg ctatattcct
    14821 taaaagtggg ttataatgtt tgttatcata gagcaaagga gcgcacatgc taaaagaaga
    14881 attcattcaa aaaagccccg tgcgaatttt tgaaaaatcc atagatggcg gtttgcacgc
    14941 gggcaatatc ggtatacttg caagtaaaaa aggtattgga aaaacctccg tgctcgttca
    15001 gatagccctg gacaagctca tgcagggcga aaaagtcatc catgtctcct tcaatgccca
    15061 cacaagctat ataatttctt ggtatgaaaa tattttcaat gaaattgcca gacgcaaaaa
    15121 tctcgagaat atagacgaga tcaaagaaca agtaatacgc aaccgaatga taatgaactt
    15181 catgcaagaa agcatttcgg ttgatcagat aatgcgaacg ataaaagccc tcatctcgga
    15241 tggcagcttc aacgcaaaga ctctaattat cgatggattc gaattcacgc gggctacttc
    15301 cgagcgattc gaaaaagtaa aagcatttct ctctgaaatg caacttgaag gatggtacac
    15361 ctgcacactt gcagagacag atcctgtgct agattctaag ggcattccga ttgtattgcg
    15421 cgatatcgaa ccatatattt cggtgatcgt tctgctcgag cccaaaaaag actatattca
    15481 tctgcgagta gtcaagaatc atgaccgcct ggagcagggc gatatgccgc ttatgctaga
    15541 tccacgaaca ttattgattg cagaagcctg atcaagactg ggccttgagc ccttctgtac
    15601 cagagaacca gttaaagtag attttttctt gacctgacac aatctcacgc acagaaacag
    15661 gcgtgattcc taccgcatct ccaaatgaag aaatagacga taagaaaccg caatcccatc
    15721 cagcttctag aaagcgctcc aaaataggcc caagctcttt atataaggag agagccttat
    15781 ctgcgctacc cattcgttgc ccatagggag gattggcgag caatactccc ctcgagccca
    15841 gaggcacgat attgcgtgca tccacctggg caaagcgtac gaactcactt atgccggcta
    15901 tggatgcatt cttacgcgcc aaggcgagtg catctttatc gatatctgaa gcccttatat
    15961 cggcgcgaat atccttccgc gcttgacctc gaaaggcgat gcgcacatct tcgattttat
    16021 tgctatctat gctggggaaa aactcgaatg cgaagcgacg ccttaggcca ggcgctacat
    16081 tacaggcata taaggcggct tcaatagcaa tggtacctga gccacagaaa ggatcaatca
    16141 aaggacgagc cctgctccat ccagcaagaa aaagcataga agccgcgatg gtctctttga
    16201 ggggagccat atgagtaaga cgacgataac cacgtttgga aagagcctct cccgaggtat
    16261 ctactacaat ctgccaccaa ttattgtcgc catatatgcg cacttcgagc gtattgccgg
    16321 tctcgggcat tgtgcgtata tgaaatcgct ccatcagcga ggcatagatg gatttctgcg
    16381 ccatagactg cagagtcgcc tgcgaatgga gtacacaata ctgcaatctc actcgttcga
    16441 tgactacttt tgtttctttt gtgcaaaagc attcccaagg aatcgaaaaa acgccttgat
    16501 agaatccatc gaaatcaaag gctttgaatt ctcctgctac tagcaatacg cgatccgcgg
    16561 tgcgtaagcc aatatttgct cttgcaagat cttccgtgag cgattgttct cttacattaa
    16621 aaaaaactct gcctgcgctc ctacccacat cgtggtagcc aagatgtgca agatcatgtg
    16681 cgagtacctt ttccagcccg attgggcatg ccgcaatggc gcgcatggaa cttcccataa
    16741 atagagtatg catggctgcc ggacgaatag caagattgat ttcccgaagc tttgacatga
    16801 tgaacaagat tctataagag atgatggaca ggatttccgt tgtgcgcctt gacacttttc
    16861 ctctatttgg gctagtatgc gaaggctttt tgggggtgta gctcagttgg ctagagcgta
    16921 tgaatggcat tcataaggtc gagggttcaa ttccctccac ctccataaat taaattatta
    16981 tagaataaat agatgctgaa tagcatcgag attaaaagtt gatccaattc cttgattgtt
    17041 acccattaga aagcggttcg ctgaaaactt atttgtcgcg attatggaca tctaaatgga
    17101 ctctatagtc gagcggtacc aattgttcca gaagcaggaa ttggcagcat aaatgccacc
    17161 ttattagtca aacaatttcg ttctgaatca aggggctcaa cttggcccta aaaatggatg
    17221 attatttaga acaagactct gtcttcaaaa cgttgacttt acagtacagc ggcggctctc
    17281 tttcgttagg ttaagcaaaa ttgccaaaaa tattttcttg acagcaaaat tttatggttg
    17341 tatgctcaaa tagtactaat gttgccggaa ttcaaggtag tagtggtcga ttaggttgca
    17401 aatcatgaag cctcggcgct ttaattgtaa gcttttgttt gcagtatgag gatgagcatc
    17461 tgcataggat tacatttcca aagctagatg tgttttgcct acaggaaaac gcagctatat
    17521 tgcatttttt atgtaacttg ggaactgttt cctaacggga ataatactgt gaggaggtat
    17581 tatgatgaaa aggtttctgt tgatggctct agcggtcttg atgatggcgt cgacctcgct
    17641 ttcagccgct cctgcgaaga tcgaggctga gctgaacctg atcacccctg ttgccagttt
    17701 tgtgagcgta gctgcactcg atgccttcaa ggtctgggct aaggccaatt atggtatcga
    17761 tgtaaagacc agctatactg ccaaaggcac tcagttggcg gcaggcctga tccgcgagtg
    17821 gggttccaac ccgcaggctg acgtgatctg gggcggagaa acggtgctct atgatatgat
    17881 ggcggctgac ggattcctga ttaagcatga ggccccgaag gccttgctgg accgaatacc
    17941 agcgactatg ggcgctccca agccaatgcc gcttaaggat cccaaaggtt tctggaccgg
    18001 tacagcgctt gagccttacg gcattgtgta caacgaggga ttggtcacaa ggcgactccg
    18061 aaccactccg ccaaaaacat gggaagacat gctcaacaat cccaagttca aggggcagat
    18121 cgctcagtgc acgcccgata actctagttc gagccatgcc acctatgagg tcatactgca
    18181 aaaatacggt tgggaaaagg gctgggaatg gctttccaag ctgggcgcct atactggcca
    18241 gttcgtcgca aagtctggtg atg
//
LOCUS       DAOSOB010000037        18048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3125_length_18048_cov_75.502084, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000037 DAOSOB010000000
VERSION     DAOSOB010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 18048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18048
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3125_length_18048_cov_75.502084"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1329)
                     /locus_tag="PLS88_05320"
     CDS             complement(49..1329)
                     /locus_tag="PLS88_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOU06795.1"
                     /translation="MSNQKAQASQKKSKMTYRWDMVFAGSMLMLCFGTVYAWSYFQVP
                     LMQTFGWNNAQVSLVFSLAIFFLGMAAAVGGVILPNAGPRKIAIIGSALFSLGYLLAA
                     LALSLKSLALLYIGYSIIGGTGLGLGYVTPVATVSKWFPEKKGLATGMVIMGFGFGAL
                     FMSKIIAPIFLRLYAGNLVNSFLTFAFLFAAITIPASLAMKDPPENMLGQQKQSKAKT
                     DISTALKQASKDIFSKRFLVVWMILFCNISAGISIIGFQSPMFQELILNRKPSSTAEA
                     LASMGATLIAVTSIFNGVGRLLWGAFSDKFGRMKAYRFLLGSEMLIFLLLIFTRSPWV
                     FAICACWILFCYGGGFGMMPATISELFGPQKMTIMYGAVLTAWSLGGVIGPQITAYIK
                     DTQPSQAIKISFIVGTVFIAIGFLFSIMISDKKK"
     gene            1533..2540
                     /locus_tag="PLS88_05325"
     CDS             1533..2540
                     /locus_tag="PLS88_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU06796.1"
                     /translation="MRAIRKAILVLAFVTLLVPVFAKAGQTLVVYSSVDEVNAKKILD
                     AFTAQTGIAVQFVHLSSGPAYARIKAESANPQADVWFGAPSENHIVAKEDKLTQPYKG
                     PNFDKLGKEFKDPEGYWRSFYMNPMAFAVNKEVLARIGAPKPTSWADLLNPAYKGQIQ
                     MPSPQSSGTAYNIVASLVVLMGEEKAFDYMKKLAPNIQTYTSSGTAPSQAVQVGQCAI
                     AIQFTPAFFEAIDKGFPIEVIFPKEGVWFEAPAISILKGAKNLEAAQTLVDWLSSVEG
                     QKVYTERKTYFYPVISGVPLGKGMPAFESLKTIDVDVLWAGEQKKRLVERWVNEVLSA
                     K"
     gene            2548..4239
                     /locus_tag="PLS88_05330"
     CDS             2548..4239
                     /locus_tag="PLS88_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU06797.1"
                     /translation="MQVSGFRARINIKRLARDPVLLAVILLVTASLAIFILYPLVEVA
                     TTSLDVGGGRLGFGVYAEVAKSAYIRKALYNSLLMSTLTAIVGTIIGFIFAFAVVRAK
                     VPLSRFFNTMAVIPIVSPPFIGALAIIMLFGNNGLISWNLLKIQNFPIYGFNGLFIAQ
                     VVTFFPVSYLTLKGVLESMNPILEDAALDLGGTRWKVFKKVTLPLAIPGIASSVLLLF
                     VETLADFGNPLILAGSRFPMLSVQAYLQIVGMYDLPKGAAISILLLVPSLIAFYLQRW
                     LVGKRKYTTVTGKPQQSTTQIVSPGARAALFVACLIIALLVAMIYFSIVVGAFAKVWG
                     YDHRPTLDHFKFVFGVGFKAVRDTLIIAGTATPFAGLLGMVIAYLVMRVKFPGRSFVE
                     SVSMLSFAVPGTVIGIGYILAFNHPPLVLTGTLTILLLNFVFRYIPVGIESGKAILSS
                     IDPSIEEAAINLGADSRKTFTKVTLPMILPAFFSGLVFAFVRGMTAVSAAIFLVSSRW
                     NLMTVHIMAQVESGNLGAAAAYSLLLVLIVIAAISLIRVTLKTQYAKFGGSMLHF"
     gene            4248..5369
                     /locus_tag="PLS88_05335"
     CDS             4248..5369
                     /locus_tag="PLS88_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06798.1"
                     /translation="MSVRLINISKEFPDPEREGGRVLAVRDFNLEVAEGEFVTLLGPS
                     GCGKTTTLRIIAGFETPTTGEVWINGKNVNNDPPNKRDTSMVFQSYAIFPHMSVEQNV
                     GFGLELKGRPKIEISREVRQIMEVMGIADLAHRRPDQLSGGQQQRVALARSIINKPSV
                     LLFDEPLSNLDAKLREQMRVEIRRIQQTFKITSVYVTHDQVEAMTVSDRIVVMDKGYM
                     MQIGTPFEIYSRPSNRFVADFIGKVNLLPVRFEREDSGRYFAKDERGLHFEAMSAVPG
                     LKSGQEALFMLRPESLSVESEESTSSSELERMILARGIVDKAVYLGSTVEYEIDVSLD
                     RPMLAVSHDPVNVGFWKPGDSVILRYSPRAAHLLPLVSL"
     gene            5577..6176
                     /locus_tag="PLS88_05340"
     CDS             5577..6176
                     /locus_tag="PLS88_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06799.1"
                     /translation="MKLVKVVGVVLVALLLFSCAPKLAQKDLDAANALFAEAQEAKAD
                     VYAPDEFLAAQDAKAALEAELAAQDAKTSGKSYKQANDLIKAFADASKKAKEAAATNM
                     EQVKNEVAQLIADVDAQYTTVQALAQAASKDTKKAAKAKLNVKDINAKVAALDEEITN
                     AKAANDAEDYAAARDQLNAVKVALDELQSTLEAAGFEAQ"
     gene            6542..7372
                     /locus_tag="PLS88_05345"
     CDS             6542..7372
                     /locus_tag="PLS88_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06800.1"
                     /translation="MVPVFRRASGGGAVYHDLGNLNWSLIVPRGLHSKEEELCLIAKA
                     ISKLGVYTHIGSRGEIFVLMGDGETEAKISGTARYFGTHNVLHHGTLLVSSDLKNLHA
                     CLGGIKILDDNSIASVPATPINLIHLIPSLTVYDVMSRLSLEIAGMNPYDIGLAEVLN
                     NHVSCVDTTLRNKGKSESDLAPFINSKDFELFKAQFGSMEWIKNRSPAFSILLSSNNR
                     RVVLRIEEGRVSEILPFEPNNLRAAHLAAHLKMRFAGLQFDFNTVEMISRIADDQFMQ
                     "
     gene            7608..9029
                     /gene="lpdA"
                     /locus_tag="PLS88_05350"
     CDS             7608..9029
                     /gene="lpdA"
                     /locus_tag="PLS88_05350"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOU06801.1"
                     /translation="MEEKEFDFIVIGAGPGGYVAAIRAAQLGLKTAIVEKNLVGGVCL
                     NVGCIPSKSLIHNARLFAEGRKLLEKTGVKLDMSGFDYEPVWKTSRLVAERLSKGVNF
                     LLKKNKVELIQGTARLAGASMVEVEGVGAIMNLRSKAILLATGSRPRIIPGFEFDEER
                     ILSSTGILMKNKLPKRLFILGAGPIGMEFAYIMNGFGVDVTIVELLPRVLPLEDEEVS
                     RIVEKEFRNRGVTIYTSAKAQNAEIMGNEVVVHIVDAQAKTIDIYADAALISVGRTPN
                     TDNLGLEKLGIKMDRGYILTGDYHETSCSGIFAVGDITTYPQLAHAASKAGEIVAERV
                     GHLLKGTPNPREHLLDRLHVPSAVYCDPQVASFGLSEASAKEKGISYQVARFPYRGNG
                     RAVAEDAPEGQVKLVFDPSNGAILGASIIGEGAAELIHEPLLASDAELTIEDIAELVH
                     AHPTFSETIMEASKAALNRAIHI"
     gene            9109..9459
                     /locus_tag="PLS88_05355"
     CDS             9109..9459
                     /locus_tag="PLS88_05355"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU06802.1"
                     /translation="MFGKHSNAGFNETSPGNRIKTLCYGEHTLLVEVHLDKDADLLRH
                     KHPYEQTGYLISGNIRMHIEEKEMELEPGDSWCIPMNAEHKVDVLKDSIVIEVFSPTR
                     EEYIKYKNDNDILR"
     gene            complement(9513..10973)
                     /locus_tag="PLS88_05360"
     CDS             complement(9513..10973)
                     /locus_tag="PLS88_05360"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU06803.1"
                     /translation="MNSISLAFGKGAMNIALNYLNKDPEKNIPKIVDLFTKFTFREDM
                     KRQLLSAKEVMEDPNNPYRGLIMRAFNELDPKIRKRFLINFVFNSGIIGQDKTAILKV
                     KYDCNIPWTILMDPTSACNMHCKGCWAAEYDKTDSMDYELLDRIIREGKELGIYFYIY
                     SGGEPMLRKDDLIKLAKVHDDCVFLAFTNGTLIDDATAKALAEAGNFGLAFSIEGFES
                     ATDFRRGKGTYQKVIQGMQNMKKYGAPFGFSACYHSKNAEAVGSEEFLDFTIEQGCMF
                     GWIFTYMPLGKDADTSLLATPEQREYMFHWVRKMRDTKPIFLIDFWNDGQYVNGCIAG
                     GRSYLHINAAGDVEPCAFIHYSNVNIKDTSLLEALRSPLFKQYAINQPFNENHLRPCP
                     LLDNSGKLAEMVKAAGAYSTQPIDREDVDDLTAKCVQAAERWAPVAEKLWAGEMPHYL
                     ESKKKSQELQDKNLEVIKKKLNKSKKKNDSNLEAAS"
     gene            complement(11074..11649)
                     /locus_tag="PLS88_05365"
     CDS             complement(11074..11649)
                     /locus_tag="PLS88_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU06804.1"
                     /translation="MPRIVDHDERKREIAQSALTLFSKQGYYDSSFSDIASACRLSRS
                     NIYNYFKNKDEIFHYAVVELLNSITQKIELIAHQEDLTLAQKLQRIYQLFTNDIEDGK
                     NASIILDLSLRLKRKDTKLVATLENSAANLRKKIESLFASENSRLPYVQVAAATTLFF
                     SLIESSITHSLFTERAFIQQNIALILQMLGI"
     gene            complement(11758..15873)
                     /gene="pgk"
                     /locus_tag="PLS88_05370"
     CDS             complement(11758..15873)
                     /gene="pgk"
                     /locus_tag="PLS88_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU06805.1"
                     /translation="MNTWENILAHLDRVRESDNEEVQIQLLIELLINLRLIGTDSSIP
                     DMRIQKSNIEDIYSEVENKTRSMLSSVRNPSSKFIYYAREVIKKFPVRQDAEEMIERI
                     RSDAEAFEENRTEDDPLRGFASDLRKEVHRRLSPRIITHFLNPYIELAAHKNPEPIIN
                     AGYVALPYTFSPDDEDEQFVDLATDLQKRLQFLWDYEEGDMATVRQHLRDNLDIFERC
                     FLRAEVEGLLGILNPEYLSSADKELDAFKRLASVKFFLKESYLESRIDLYDLILLDLG
                     LGRLIFLLANDLTNNFFAEVTPRNIRDALEVMRELLTISSIKGLRIQNVKLRQNELGE
                     LRESSVSDFIRLKHSLEAISSELQQYIQSEIIDEMTGSLNQILENYRVPTSKLSQIKT
                     RFFNNFIRRTQIHVLSEFVEKVSTAVDKELERQQGEGQLYLRYQRLLEKSSFSEYIEE
                     KGIDAYIAVTWRKPEQWLRPFLGGKGNSIIDMAQIGLPVPPAFVLSYPLLAAINQNTD
                     QIRTGIIAKLRELEMMTGALLGNEDRPLLLSVRSGALTSLPGMMSTIMNIGLVPRVRA
                     ALSQKHGADFIDTLYLRFLENCESALSLGQQNPRQRFKDSAAQSVAHSATLNKNRGSD
                     LYLQRKKTEVLESFLAAYLGPSFLTDEEEQLFTCIELVYDSRTSKTVNLYSKTLASHE
                     TIETAVTIQQVVFGNLNENSLSGVVMTRNPITGEDTLFGDFKIRAQGEEVVMGSLDTL
                     PIEEINPKIAHQLSEYKKKLIDFYHQDLDIEFTVEDGVLYLLQARAARLGSFAQLIAA
                     TDFLKRGIITLDKYKEHIESLELAYANVPLPRADFRFRQWVPPLATGIPINGGVVSGT
                     LIITIERLKEAEKRRESVIFFAVNTKPTDFHIFNLSSAIITVYPGRTSHAAITAMTLN
                     KACIVGCSDIEIDYENRRVIFHGAGDIALTEGERITIDGNAGAVYRGVAPISDTCMPV
                     YRIREAILNAQSARETAQIVETMISTKMKALQRESSLQKKSLAEVGDLHNRYVLLRVD
                     ANIDLSETSQPEVVDMRVAIMIPVIKELLERGATPIICSHRGDPGTHSMPGISREQIY
                     EAYSLLPIAQKLESLMGRVVKFHHVSVGSSGILISRKDIIHGHINMLENLRYATGEKD
                     NDEAFARSLAELSDGIFINDAFNVCLRRHASIVSVSRFTQANIAGPTVIQELKILEKV
                     LDKVERPFVAVFSGEEIEAQYGAMASMLPRVDTMAMILPNNTAPLAPFSHFYPAKIIV
                     IDPKDPAWINQSCALEAMLENARTILWAGPAGLSSKFAQKLEALPPYILSLHRAMEKA
                     SMTIICSETEKHFAGKTIENIHISTGPRAFLEYLERLSLPGIIALDDALRQ"
     gene            16038..16928
                     /locus_tag="PLS88_05375"
     CDS             16038..16928
                     /locus_tag="PLS88_05375"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOU06806.1"
                     /translation="MRVLNFGSINIDYVYSVDEFVKPGETKAARSLSVFAGGKGLNQS
                     LAMAKAGLKVVHAGKIGKEGEFLVKTLKDGGVDTKLIEISDVPTGHAIIQVDDKGRNC
                     ILLFGGANQDIGEAYIDRVLVGFGHGEMLILQNEISSMSSIIAKAKTKGMTIVFNPSP
                     YSSLVPNYPLELVDIFIMNEIEASGLSGEHDPDKAAGLLMRNFPQALIVITLGEAGSM
                     CAHKGEILRQKAYRVKVVDTTAAGDTFSGYFIAGLAEGMDIQKALDLAARAASICITR
                     KGAADSVPWRKELEVGDIRI"
     gene            16937..>18048
                     /locus_tag="PLS88_05380"
     CDS             16937..>18048
                     /locus_tag="PLS88_05380"
                     /inference="COORDINATES: protein motif:HMM:NF018234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU06807.1"
                     /translation="MKLYSNLRVLFVAFFMLCNLVLVSAQQNGLTQQVQQTTPSHKDI
                     QPLVGEWEGKLKIGASSLRIVFHITDSGSGYVATMDSPDQGARGIPVSRVRIEGKRVG
                     LEVSSIGGSYTGALDEPGNRLEGFWKQSGMSFPLILVKQSPPEIHTEQKQSQDLATSF
                     PYSIKEVQFNGGAEGVRLSGTLTMPQGQEPFPAVVLVSGSGPQNRDEEILGHKPFFII
                     ADYLTKRGIAVLRYDDRGVGASTGDYQTATTFDFAADAEAALAFLVQQEGIDVNNIGI
                     IGHSEGAIIASIVAAGNEAGYASTYCGGHESTCDACGSSGFNAICEFSRPTFIVLLAC
                     PGVRGYELLIMQNAAIGRASGLSEEQIIEANNLNRRL"
BASE COUNT         4877 a         3811 c         4271 g         5089 t
ORIGIN      
        1 gagtccttcc cctgctagac aataggccgc tttcttttag agggcggcct atttcttttt
       61 gtcagaaatc atgattgaaa aaagaaaacc tatagcgata aaaacagttc ctacgataaa
      121 ggaaatcttg atggcttgag atggctgagt atccttgata tatgccgtaa tctgagggcc
      181 gattactcca ccaagcgacc atgcggtcaa tacagcaccg tacataatag tcattttttg
      241 cggtccaaac agctcagaaa ttgtagcagg catcatccca aatcctcctc catagcagaa
      301 aaggatccag caagcgcata tagcaaatac ccatgggctc cttgtaaata taagtaagag
      361 gaagatcaac atttcactcc caagcaaaaa acgataagcc ttcattcttc caaatttgtc
      421 cgagaaagct ccccagagca atcttcctac accattgaag atagaagtca cggcaataag
      481 agttgctccc atcgaggcaa gagcttccgc tgtggaagaa ggctttctat tgagaataag
      541 ctcctggaac ataggtgact gaaagccaat gatagatatt cctgcgctga tattgcagaa
      601 taggatcatc cataccacaa ggaatctctt tgaaaaaata tctttcgatg cttgtttcaa
      661 agctgtagaa atatctgttt ttgccttgct ttgcttttgt tgtccaagca tattctctgg
      721 tgggtctttc atggcaagag aagcaggaat tgttatagca gcaaaaagaa atgcaaaagt
      781 aaggaaggaa tttacgagat tcccagcata caatcgcagg aaaatgggtg ctatgatctt
      841 gctcataaaa agagcgccaa agccgaatcc cattattacc ataccagttg caagaccttt
      901 tttctctggg aaccatttgg aaaccgtagc cactggagtt acgtatccaa ggcctaatcc
      961 cgtgcctcct atgatgctat atcctatata taataaagct aaggatttga gagagagcgc
     1021 gagggccgca agaagatatc cgagactaaa aagagcgctt ccaattatgg caatttttcg
     1081 tgggcctgcg tttggaagta ttactccgcc tacagcagca gccatgccaa ggaagaatat
     1141 agccaatgaa aatacaagag aaacctgagc attattccat ccaaaggtct gcataagagg
     1201 gacctggaag tagctccaag catagactgt accaaagcag agcattagca tggagcctgc
     1261 aaagaccata tcccatctat atgtcatttt agattttttt tgtgaggctt gagccttttg
     1321 atttgacatg attgtatccc ttccgttatt ggtattcagc atgcgctaga atccatatcc
     1381 agtcaatgcc aaactcaaag cagccgctgg cttggaataa aataagcata cctatatatt
     1441 ctgggtgcac tacaattttt tctcattgca ggcgctccga aaagattata aaatactatc
     1501 cattacatat ttttcgattt ggaggcgata tcatgagagc aattcgcaag gcaattctag
     1561 tattggcatt tgtaactttg ctagtgccag tctttgcaaa ggccggtcag actctagtcg
     1621 tttattcaag tgtcgatgag gtaaacgcaa aaaagattct agatgcattt acagcgcaaa
     1681 ccggcatcgc tgtacagttc gttcatctat catccggacc agcttatgcg agaatcaagg
     1741 ccgaatcggc aaatccccag gcagatgtat ggtttggcgc tcctagcgaa aaccatatcg
     1801 tagcaaagga agataagctc actcagccat ataaggggcc aaactttgat aaactcggca
     1861 aagagttcaa ggatcccgag ggatactggc gcagctttta catgaatccc atggcttttg
     1921 cggtgaacaa agaagttctt gctcgtatag gagctccaaa acctacgagt tgggctgatt
     1981 tgctcaatcc ggcgtataaa gggcaaatac agatgccatc tcctcaatct tctggaaccg
     2041 catataatat cgttgcttcg ctcgtcgtgc ttatgggcga agaaaaagca ttcgattata
     2101 tgaaaaagct tgctccaaac attcagacct atacatcctc aggtactgca ccatcgcaag
     2161 ccgttcaggt tgggcaatgt gcaatagcaa ttcaatttac accagcgttt ttcgaagcaa
     2221 tcgacaaggg cttccccata gaagtcattt ttccgaagga aggtgtctgg tttgaagcgc
     2281 ctgccatctc catccttaaa ggtgccaaga atcttgaagc agcacagacc ctagtggatt
     2341 ggctttcctc tgttgaaggt caaaaagtct atacagagcg aaagacatat ttctacccgg
     2401 taatttcagg cgttccgcta ggcaagggaa tgcccgcttt tgagtctctt aagaccatcg
     2461 atgtcgatgt tctctgggct ggcgagcaga agaagaggct tgtagagcgc tgggtcaacg
     2521 aagtgctaag cgcaaagtga gcaattgatg caagtatctg gattccgggc gaggataaat
     2581 atcaagcgtc tcgcccgcga tccggtgcta ctggccgtta tcttgctggt caccgcatcg
     2641 cttgcgatat ttatacttta tcctttggtc gaagtagcca ctacgagcct cgatgttggc
     2701 ggtggcagac ttggatttgg agtatatgct gaagttgcaa agtcagccta tattcgcaag
     2761 gctttataca acagcctttt gatgagtact ttaacggcga tcgtcgggac aattattggc
     2821 tttatttttg ccttcgcggt tgtgcgtgct aaagttccat tgtcaagatt ttttaatact
     2881 atggcagtga ttcccattgt atctccacca tttattggcg ccttagcgat cataatgctt
     2941 tttggcaata atgggctaat cagctggaac cttctaaaga ttcaaaattt tcccatttac
     3001 ggattcaatg gactttttat tgctcaggta gtcacctttt tccccgtatc ttatttgact
     3061 ttgaaaggtg tactcgaatc gatgaacccg atcctagagg atgcagcgct ggatcttgga
     3121 ggaacaaggt ggaaagtctt caaaaaagtt actttaccgc tagctattcc gggtatcgca
     3181 agttctgtgc ttcttctttt cgtcgagacc cttgcggatt tcggtaaccc tcttattctt
     3241 gcggggagca gatttcctat gctatcggtc caagcatatc tgcagatcgt aggtatgtat
     3301 gatcttccta aaggtgccgc catctcgatt ttgcttctag tgccatcgct aattgctttc
     3361 tatcttcagc gatggttagt gggaaagcgc aaatatacaa cggtgacggg aaaaccgcag
     3421 caatccacga cacagattgt aagcccagga gccagagcgg ccttgtttgt ggcttgccta
     3481 ataatcgctc tattagttgc gatgatctat ttttctattg ttgtcggagc ttttgccaag
     3541 gtgtggggtt acgatcatag acctactcta gatcacttta aattcgtatt cggcgttggg
     3601 ttcaaagcag tgcgcgacac actcataatt gctggcaccg ctacgccttt tgcggggctt
     3661 ctgggcatgg tgatagcgta tcttgtcatg cgagtcaaat ttcctggccg gagtttcgta
     3721 gaatcggtga gcatgctgag ctttgcagtg cccggtacgg ttattggcat aggatatatc
     3781 cttgccttca accatcctcc tttggtattg acggggacat tgaccattct ccttctaaat
     3841 tttgtattta gatatattcc agttggaata gaatcgggca aagcgatcct ctcgtctata
     3901 gatccttcga tcgaagaagc agccatcaac ttgggagcgg attcgcgcaa gaccttcact
     3961 aaggtcactc ttccaatgat tttgcctgct tttttttcag gtcttgtttt tgcctttgtc
     4021 cgcggaatga cagccgttag cgctgcgatt ttccttgttt cctcgagatg gaacctgatg
     4081 acggttcata tcatggcgca ggtggaatcc ggaaatctag gagcagcagc ggcatatagt
     4141 cttttgcttg tattgattgt catagcagcg atatcgctta taagagtgac cttgaagaca
     4201 caatatgcaa agttcggtgg ctcaatgctt cacttctagg agaatacatg agtgtcaggc
     4261 taatcaacat ttccaaggaa tttcccgatc ccgagcgtga aggaggcaga gtgctcgccg
     4321 tgagggattt caatctcgaa gttgccgaag gtgaatttgt aaccttgctt ggaccttctg
     4381 gctgtggcaa aacgacgacc ttgcgcataa tcgctggctt cgagacgcct actacaggcg
     4441 aagtatggat aaacggcaag aatgtcaata atgatccacc taataagcgc gatacatcga
     4501 tggtatttca gagctatgct attttccccc acatgtcagt ggaacagaat gtgggttttg
     4561 gtctggaact taaaggtcgc cctaaaatcg aaatctcgcg cgaagtacgc cagatcatgg
     4621 aagtaatggg aattgcggat ctcgctcatc gacgaccaga tcagttatct ggaggacaac
     4681 agcagcgagt ggctcttgct agatcgataa tcaataaacc aagcgtgctc ttattcgatg
     4741 agccgctatc caatctcgat gcgaaacttc gcgaacagat gcgagtagag attcgtagaa
     4801 ttcagcagac tttcaagatt actagtgtat atgtgactca tgatcaggtc gaagcaatga
     4861 cggtgtctga tcggatcgtc gtaatggaca agggatacat gatgcagatt ggtactccct
     4921 ttgagatata ctctagacct tctaataggt tcgtagcgga tttcattgga aaagtgaatt
     4981 tgcttcctgt aagattcgag cgagaggatt ctggaaggta ttttgcaaag gatgagagag
     5041 ggttgcattt tgaggcaatg tcggcggttc cgggcttaaa gtctggtcaa gaagcacttt
     5101 ttatgttaag gcctgaatct ctctctgtcg aaagtgaaga aagcacatct tcatccgagt
     5161 tggaacgcat gattctagcg cgcggtattg tcgacaaggc tgtatatctg ggatcgacag
     5221 tagaatatga gatagatgtt tcactagatc gacctatgct tgctgtttcg catgaccctg
     5281 taaatgtagg attctggaaa ccaggagact cagtgatact gaggtacagt cctcgcgcag
     5341 cccatctgtt gcctcttgtg agtctttgaa aaagtgtcag gaattttgct ataaatatgc
     5401 taaatatgat gtatagaagc catatattct tatctcacat gaaattatga aaataatctc
     5461 tatggccgca tagcttgaca tattctgtaa aaatagataa attatcgcaa tagaaagcag
     5521 acgcagcgct ccgatggagt gcaagcgctg taatttgaat tttattggag gattgtatga
     5581 agctggtaaa ggtagtcggt gtcgtacttg ttgctctttt gttgttctca tgcgcgccga
     5641 agcttgctca gaaggatcta gatgcagcca atgcattatt cgctgaagct caagaagcta
     5701 aggccgatgt ctatgctcca gatgaattct tagcagctca ggatgccaag gccgcactcg
     5761 aggcggagct cgcagcccag gatgccaaga cctctggaaa gtcctacaag caggcgaatg
     5821 accttataaa agcttttgcg gatgcaagca agaaggctaa agaagctgcg gctacaaaca
     5881 tggaacaggt aaaaaatgaa gttgcgcagc taattgcaga tgttgatgct caatacacca
     5941 ctgttcaggc tcttgctcaa gctgcatcaa aggatacaaa aaaggcagca aaggcaaagc
     6001 tcaatgtaaa agatatcaat gcaaaagtcg ctgcgcttga tgaagagatt actaatgcaa
     6061 aggcagcaaa tgatgccgaa gattacgcag ctgctcgtga ccagctcaat gcagtcaagg
     6121 tcgctctaga tgaactccag tcaactctcg aggctgctgg atttgaagct caataaacat
     6181 agagtttctc aagtgctatc atgagctaga gaatagagga gcttgcaaag ctcaggtatt
     6241 caggtgatag ctcctgcatt tcaataaaag accgctccta cagggcggtc ttttagttta
     6301 ataatgctgg agatcgaaat gcgttggaat tcactaggct ttcttatata ttaaaagcat
     6361 gcctagattt gctggatcat atagaattct agcagagagc ccacttgatg taatttcttt
     6421 cgaagaatgc ctcgtaagat attggcgagg aaataatgcg attcttcttt tctatgttaa
     6481 ccccccaagc gtgataatag gaaggaatca aaattattgg cgggaagtgg cacctaattg
     6541 tatggttcca gtatttaggc gtgcctcggg gggaggcgca gtatatcatg atcttggcaa
     6601 cttgaattgg tctttgattg ttccgagggg gcttcattcg aaagaagaag agctgtgttt
     6661 gatagcaaaa gcgatttcca aactcggagt ttatactcac ataggatcaa gaggcgagat
     6721 ttttgtttta atgggcgatg gtgaaacaga agccaagatt tctggtacgg caaggtattt
     6781 tggtacgcac aacgtgttac atcatggaac attgcttgtg tcctcagatc tgaagaatct
     6841 tcacgcctgc ttaggaggga tcaagatatt ggatgataat tcgattgcct ctgtgccagc
     6901 aactccgatc aatcttattc acttaatacc ttcgctcacg gtttacgatg taatgtcaag
     6961 gctttctctt gaaattgcag gaatgaatcc ctatgatata ggacttgcag aagttctgaa
     7021 taaccatgtg tcctgcgtcg atacaacatt gaggaataag ggaaaatcgg agagcgatct
     7081 ggcaccattc ataaactcta aggatttcga gttattcaag gcgcagtttg gatcgatgga
     7141 atggattaaa aaccgaagcc cagctttttc aatattgctc tcaagcaata acagaagagt
     7201 agttctaaga attgaagagg gcagagtgtc ggagatactt cccttcgagc caaacaatct
     7261 tcgagctgca catcttgctg cacatcttaa aatgagattt gctggtctac aattcgattt
     7321 caatactgtt gagatgatta gcaggattgc cgatgaccaa ttcatgcagt gagccaaata
     7381 tcatagagaa tagacttgag caatgaaccc aatatcctaa aagatagatt cgtaggccaa
     7441 gccaaatatc acatgacaat tccgcgcttt aagcttcaat tcttcttgac cttaagctat
     7501 gacagtctta gcattggtag catgattggt ccgcctaatt tgctgacctt tcgaaaatac
     7561 caaaaaagcc tataatggcg ctcgacgaaa tttcaggagc aacaattatg gaagagaaag
     7621 aattcgattt tattgtaatt ggcgcggggc caggcggtta tgttgccgcg attcgtgcag
     7681 cacagctagg gcttaagact gcaattgtgg aaaagaatct tgtaggagga gtctgcctca
     7741 atgtagggtg cattccttcg aaatcgctga ttcacaatgc acgacttttt gccgaaggca
     7801 gaaagctgtt agaaaaaacc ggtgtaaagc tagatatgtc tggttttgat tatgagcccg
     7861 tatggaaaac ttcgcggctt gtcgctgaaa ggctttctaa aggagttaat tttctattga
     7921 agaaaaataa agtggaactc attcaaggga ccgcaaggct cgctggagca agcatggttg
     7981 aggtggaagg cgttggcgct atcatgaatc ttaggtctaa agccatcctt ctagctactg
     8041 gttcaagacc aagaataatt cctggtttcg aattcgatga agagcgtatt ttgtcttcta
     8101 ccggtatatt gatgaagaat aaacttccta agcgcctttt tattctaggc gctgggccga
     8161 taggcatgga atttgcctat attatgaatg gttttggtgt agatgtgacc attgtagagc
     8221 tcttgcctag ggtactgcct ttggaagatg aagaggtttc tagaattgta gaaaaggaat
     8281 tccgtaatcg aggtgtgact atctacacta gcgcaaaggc tcaaaatgcc gaaattatgg
     8341 gtaatgaggt agttgttcat atagtggatg cacaagccaa gacgatagat atctatgccg
     8401 atgcagcgct tatatcggtt gggcgtacgc ccaataccga taatcttggg cttgagaagc
     8461 tcggaatcaa gatggatcgg ggctatattc taactggtga ttatcatgaa acctcatgtt
     8521 cgggcatatt tgcggtgggt gatattacaa cataccctca gctagcgcat gcagcttcca
     8581 aagctggcga gattgtggcc gagagggtcg gccatctctt aaagggaact ccaaatccgc
     8641 gagagcacct gctagacaga cttcatgtgc caagcgctgt ttactgcgat cctcaggtag
     8701 cttcttttgg tttgtccgaa gcgagcgcaa aggaaaaggg cattagctac caggttgcaa
     8761 ggttcccata tcgaggcaat ggacgcgcag tagcagaaga tgctccggaa gggcaagtaa
     8821 agcttgtttt tgatccatcg aacggtgcga ttctcggtgc ctcgattata ggcgaaggag
     8881 cagccgaatt aatccatgaa cctctcctcg cctcggatgc tgagctcacg atcgaagata
     8941 tcgcagagct tgtgcatgct catccaacat tctcagagac gattatggaa gcttcgaaag
     9001 ctgcactaaa tcgagctatc catatctaga gcgatagtat ttctttgctt atgaacgata
     9061 tttattaaca agaattatta tacgagtaat tctgatagga ggaacacaat gtttggaaaa
     9121 cattcaaatg ccggcttcaa cgagacctcg cctggaaatc ggatcaagac tctttgctat
     9181 ggcgaacata ccctgcttgt cgaagtccac ttagataaag atgccgatct tttaaggcat
     9241 aaacacccat atgaacaaac tggttatctt atatcaggca atataagaat gcacatcgag
     9301 gaaaaagaaa tggaactaga gcctggcgat agctggtgca tacctatgaa tgccgaacat
     9361 aaggtggatg tgctaaagga cagcatagtc atcgaggtct ttagccccac acgcgaagaa
     9421 tacatcaagt ataagaacga taatgatatt ctacgatgaa cattggttta tcttagaata
     9481 tcattcagct tccaatccgt gagaagaaat gctcaggatg ccgcttctag atttgagtcg
     9541 tttttctttt tgctcttatt gagcttcttc ttgattactt cgagattctt atcttgtaat
     9601 tcttgactct tctttttgga ttcgagatag tggggcatct caccagccca gagcttttca
     9661 gccacaggcg cccatctttc ggcagcttgt acacactttg ctgttagatc atccacatct
     9721 tccctatcta ttggctgcgt cgaatatgct ccagcagcct ttaccatctc tgctagtttt
     9781 ccgctattgt ccaaaagtgg gcatggtctt aagtgatttt cgttgaatgg ttgattgatg
     9841 gcatattgct tgaacaaggg tgagcgcagt gcttctaata gcgatgtatc cttgatgttt
     9901 acattggaat agtgaatgaa agcgcaaggt tcgacatcgc cagcagcgtt gatatggagg
     9961 taacttcttc cgcctgcaat gcaaccattt acatactggc catcattcca gaaatcgata
    10021 aggaaaattg gtttggtatc gcgcatcttc ctgacccaat ggaacatgta ctcgcgctgt
    10081 tctggtgtgg ctaagagact agtatctgca tctttaccta ggggcatata tgtgaatatc
    10141 cagccgaaca tacaaccttg ttcgatcgta aaatcgagga attcttcgct acctaccgct
    10201 tctgcattct ttgaatgata gcatgcacta aatccaaatg gggcaccata ttttttcata
    10261 ttctgcatgc cctgaatgac tttctggtag gtgccctttc ctcgtcggaa atcagtagca
    10321 ctttcaaaac cttcaatgct gaatgccagt ccaaaattac cggcttctgc aagtgccttt
    10381 gctgtagcat catcgatcaa agtaccgttt gtaaatgcca agaatacgca gtcatcatga
    10441 acctttgcaa gttttattaa gtcgtctttt ctcagcattg gctccccgcc cgagtagata
    10501 tagaagtaaa ttccaagttc ttttccttcc cttatgatgc gatcgagaag ctcataatcc
    10561 atggaatcgg tcttatcata ttctgcggcc cagcaaccct tgcagtgcat attgcatgca
    10621 cttgtgggat ccataaggat agtccatggg atattacaat catatttaac tttcagaata
    10681 gcagttttat cttggccgat tattccagaa ttgaatacaa aattgattag aaagcgtttg
    10741 cgaattttcg gatcgagttc attgaatgcc ctcattatga gacctcgata gggattgttg
    10801 ggatcttcca tcacctcttt tgcactcaat agctggcgct tcatatcttc ccggaaggtg
    10861 aatttcgtga atagatctac aatttttggg atgttcttct caggatcctt gtttaggtaa
    10921 ttaagagcaa tattcattgc gcctttccca aacgcaagtg aaattgaatt catatttctc
    10981 tcctctttct ttctattgtc cgcaagagtg ctttgtcaat acaacgacaa ggaatctcta
    11041 tgttctcttt caacataaaa ccttctccta caatcatatc cctagcattt gcagaatcaa
    11101 agctatattc tgctgaataa aagctctttc ggtaaaaaga gaatgcgtaa tactgctttc
    11161 tataagggag aaaaagagcg tcgtagctgc tgcaacctgg acataaggca gcctagaatt
    11221 ctcgctagca aagagactct cgattttttt gcgaagattt gcggcagaat tctcgagagt
    11281 agccacaagc ttggtatctt ttcgcttgag tctgagtgaa aggtcaagga taatcgaagc
    11341 gtttttaccg tcctcaatgt cgttggtaaa aagctggtat atccgctgaa gtttctgagc
    11401 gagtgttaaa tcctcctgat gagcaataag ctcgattttt tgagtgatgg agttgagcaa
    11461 ttcaacaacc gcataatgaa agatctcatc tttattttta aagtaattat atatgttaga
    11521 gcggcttagc ctgcaagctg atgctatatc actaaagcta gagtcatagt agccttgctt
    11581 tgaaaatagc gttagtgccg attgagcgat ttctcgtttt ctttcgtcat gatcgacgat
    11641 cctcggcata agctttgcct ccaatttcaa aattctgagt aattatttat tttgacatct
    11701 atgtcaaata taattattac aaaaaattct gtcaataatt aatagctgtt tttatcttta
    11761 ctgtctcagt gcatcatcaa gtgcgataat gccaggcaag gaaagccgtt cgagatactc
    11821 caagaaggca cgggggcctg tggagatatg gatattttca attgtttttc ctgcgaaatg
    11881 cttttctgtt tctgaacata tgatggtcat tgaagctttt tccatcgctc ggtgcagcga
    11941 aaggatatag ggcggaagag cttctagttt ctgtgcaaat ttggatgata agccagcagg
    12001 tccagcccaa aggatggtgc gggcgttttc gagcatagct tccagagcgc aactctggtt
    12061 aatccaagct ggatccttag gatcgatgac aattatctta gcagggtaga aatgagagaa
    12121 tggcgcaagg ggcgcagtat tattgggaag aatcattgcc atggtatcga ctctaggaag
    12181 cattgatgcc atcgcgccat actgcgcttc gatctcctcg ccgctgaata ccgcaacgaa
    12241 tggccgctca accttgtcta gcactttttc gaggatttta agctcttgta tgacggtagg
    12301 accggcaatg ttagcttgtg taaagcgaga tacgcttacg atgcttgcat ggcggcgcag
    12361 gcatacattg aatgcatcat tgatgaatat gccatcgctc agctcagcca aggagcgggc
    12421 aaaggcttca tcattgtctt tttcacccgt tgcatatcgg agattttcca gcatattgat
    12481 atggccatgg atgatgtctt tccttgatat taatatgccg gatgagccaa cgctcacatg
    12541 gtgaaatttg acgacccttc ccatgagact ctctagtttc tgcgcgattg gcagaaggct
    12601 atacgcttcg tagatttgtt cgcgggagat gcctggcatg ctatgggtcc ccggatctcc
    12661 acggtgcgag cagatgattg gcgttgcgcc acgctctaag agctctttaa tgacaggaat
    12721 cattattgca acacgcatgt ccacaacttc aggctgggat gtctcgctga gatcgatatt
    12781 tgcgtcaaca cgcaggagca catagcggtt atgtagatcg cccacttctg caagactttt
    12841 cttttggaga cttgattcgc gttggagtgc cttcatcttt gtagagatca tcgtttcgac
    12901 gatttgagcc gtttctctgg cagactgtgc attcaggatt gcctcacgga tgcggtagac
    12961 gggcatgcat gtgtcggata taggagcaac acctctatac accgcacctg cattgccatc
    13021 gatagtgatt cgttctcctt cggttaaggc aatatcgcca gcgccatgaa agataaccct
    13081 gcggttttcg tagtctattt cgatgtctga gcaaccgaca atgcaggcct tattcaaggt
    13141 cattgctgtt atggctgcat gcgatgtgcg tcctggatac actgtgataa ttgcacttga
    13201 gagattaaag atgtggaaat ccgtcggttt tgtattgacg gcaaagaaaa ttacgctttc
    13261 tcggcgtttc tccgcttcct tgaggcgttc gatagttatt atgagtgtcc ccgagacaac
    13321 cccaccattg atcggtatgc ctgtagccag aggcggaacc cactgcctaa accgaaaatc
    13381 tgcccttggt agcggaacat ttgcgtatgc aagctctaag ctttcgatgt gttccttgta
    13441 tttatcgagt gttattattc cacgcttcag aaaatccgta gcagctataa gctgtgcaaa
    13501 agacccgagt cttgctgctc tagcctgtaa taggtacaaa acgccatcct cgaccgtgaa
    13561 ttcgatatcg aggtcctgat ggtagaagtc gattagcttt ttcttgtatt ccgagagctg
    13621 atgagcgatt tttggattga tctcttcgat cggcagagta tccaaagagc ccatgacgac
    13681 ttcttcccct tgagctctga tcttgaaatc gccaaatagc gtatcctcac cagtgatggg
    13741 gttgcgagtc attacaacac cactcagact attctcattt agatttccga aaacgacctg
    13801 ctgaatagtc accgcagttt ctatagtttc atgggaagcg agagttttag aatacaggtt
    13861 gacggtcttt gaggtacgtg aatcgtacac aagctcgata caggtaaaaa gctgctcttc
    13921 ttcatcagtc aggaaggaag gccctaaata tgcagctagg aaagactcga gcacttccgt
    13981 ctttttgcgc tgcagataaa ggtctgagcc tcgattcttg tttagcgtag cgctatgcgc
    14041 aacgctttgg gcggcgctgt ctttgaatct ttgtctggga ttttgctggc caagactcaa
    14101 agcgctctcg cagttttcca aaaagcgcag gtataaagtg tcgatgaaat cagccccatg
    14161 tttttgggat agtgctgctc gcaccctggg cacaaggcca atattcatga ttgtcgacat
    14221 cataccgggc agcgaggtga gagcccccga cctgacagag agaagcaatg gtctatcctc
    14281 attacccaac agtgcgccag tcatcatttc tagttcgcgc agcttagcaa taattcctgt
    14341 cctgatttga tcggtattct ggttaatagc agcgagaaga ggatagctga gaacaaaggc
    14401 tggaggaact ggaaggccta tctgcgccat atcgatgata ctattgcctt ttccgcccaa
    14461 aaaggggcgc agccactgct ccggtttgcg ccaggttacc gcaatgtacg cgtcgatgcc
    14521 tttttcttcg atgtactccg aaaaggagct cttttccagc agacgctgat agcgtagata
    14581 gagctgaccc tctccttgtt gccgctccag ctctttgtct acggcggtgg agactttctc
    14641 gacaaattcg cttaacacat ggatttgggt gcggcgaata aaattgttga aaaaccttgt
    14701 ctttatttga gaaagtttag atgttggaac cctataattt tctagaattt ggttaaggct
    14761 acccgtcatt tcatcgatta tctcggattg aatatattgt tgtagttcgg aagagattgc
    14821 ctcgaggcta tgcttgagcc taataaaatc ggagaccgac gattcccgca gctctccaag
    14881 ttcattttgc cgtaatttga cattctggat tctaaggcct tttatgctag aaattgtaag
    14941 caactcgcgc ataacctcaa gcgcatcgcg gatatttcta ggggttactt cagcaaaaaa
    15001 attgttggtt aaatcgtttg caagaagaaa gataaggcgt ccgagtccta ggtcgagcag
    15061 aatgaggtca tagagatcga ttctggattc caggtacgat tcctttagaa agaattttac
    15121 gcttgcgagg cgcttaaaag catcaagctc tttgtctgcg ctggaaagat attcgggatt
    15181 tagaatacct agtaggcctt cgacctccgc gcgaagaaaa catcgttcga agatatctag
    15241 attatcgcgc aaatgctgtc taactgtggc catgtctcct tcttcataat cccagaggaa
    15301 ttgcaggcgc ttttgaagat cggtagccag atcgacaaac tgttcatctt catcgtcggg
    15361 cgagaatgta taggggaggg cgacatagcc tgcatttatt attggctcgg gatttttatg
    15421 tgcagctaac tctatatatg gattgagaaa atgggtgata atacgtggag aaaggcgacg
    15481 atgcacttct tttcgcagat cgctggcaaa gcccctgagc gggtcgtctt cagtacgatt
    15541 ctcttcgaag gcctcagcat ccgagcggat tcgctcgatc atttcttcgg catcctggcg
    15601 aacagggaat ttttttataa cttctcgcgc ataataaatg aattttgaag aaggattcct
    15661 tacgctggaa agcatactgc gggtcttatt ttcaacttcg ctatagatat cctcaatatt
    15721 gcttttttgt attcgcatgt cgggaataga gctgtctgtc ccgatcagcc ttaggtttat
    15781 aagaagctcg atgagaagct gtatctgcac ctcttcgttg tcgctttcgc gcactcggtc
    15841 gagatgagcg aggatgttct cccatgtgtt caaaagggaa tgttttggat caggtgtcac
    15901 aattaaagta tttgaatgat tctgacggat tgtcaatgtt caaagcagaa attcagctta
    15961 tacgcgtgat ataaggtcga tgcaggattc ggagtcttat gatatgatgg atgcgaaaat
    16021 tgtacaggag cacacggatg agagtcctca atttcggttc catcaatata gattatgtct
    16081 acagcgtaga tgaatttgtc aagccaggcg agacaaaagc agcgcgctcg ctttcggtct
    16141 ttgccggcgg caagggatta aatcaatccc tggcaatggc taaagcgggg ctaaaggtgg
    16201 tacacgcggg aaaaatcgga aaagagggag agtttctggt caagacgcta aaagatggtg
    16261 gtgtggacac taagcttatt gagatttctg atgtgcctac ggggcatgcg attattcagg
    16321 tagatgataa aggccgaaat tgtattctat tgttcggtgg ggctaatcag gatatcggcg
    16381 aagcgtacat cgaccgcgtt cttgtgggct tcggccatgg tgaaatgctt attcttcaaa
    16441 acgaaatatc ttctatgtcg agcataattg ccaaggcaaa gaccaaaggt atgactattg
    16501 tcttcaatcc atctccatat tcgagtcttg ttccgaatta tcctctcgag ctagttgata
    16561 ttttcattat gaatgaaatc gaggcgtcag gtctttcggg cgagcatgat ccagataaag
    16621 cagccggact tcttatgcgc aattttccac aagcgctgat tgtgatcacg ctaggagagg
    16681 ctggatcgat gtgtgctcac aaaggagaaa tcctgcgaca aaaggcctat cgagttaaag
    16741 ttgtagacac aacagcggca ggagatactt tttctggata tttcatagct ggactggctg
    16801 aaggaatgga tattcagaaa gctcttgacc ttgctgcgcg tgccgcatct atctgtatta
    16861 ctagaaaggg agcagccgac tcggtgccct ggcgtaaaga gcttgaggta ggagatataa
    16921 ggatctagag gggattatga aattgtattc taatttaaga gtcttatttg tggcattttt
    16981 catgctgtgc aatctggtat tggtatctgc ccaacagaac ggactaactc agcaagttca
    17041 gcagaccact ccatcccata aagatattca gcctttagta ggcgaatggg aagggaagct
    17101 caagattggt gcatctagtc tgagaatcgt ttttcatatt acggattcag gctctggcta
    17161 tgtggccacg atggatagcc ccgatcaagg ggcaaggggt attcctgtgt cgagggttcg
    17221 catagagggc aaacgtgtag ggctcgaggt ttcatctatt ggaggtagtt atacaggagc
    17281 tctggatgag ccaggtaatc ggctcgaggg tttctggaag caatctggta tgtcgtttcc
    17341 gctcattctc gtaaagcaat cacctccaga aatccatacg gaacagaagc aatcgcaaga
    17401 cctagcgact tcatttcctt atagcataaa agaagtccag tttaatggag gagcagaagg
    17461 tgtacgactt tctggtactc tcactatgcc tcaggggcaa gagccttttc cagctgtagt
    17521 gttggtctct ggttctggtc cgcagaacag agatgaggag attcttgggc acaagccttt
    17581 tttcataatc gccgattatc tgactaaaag aggcatagcc gtgctccgtt atgatgatcg
    17641 cggagtagga gcttccaccg gtgactatca aacagcaaca acatttgatt ttgcagcaga
    17701 tgctgaggca gctctcgctt ttcttgtgca gcaagaaggg atcgatgtga ataatatcgg
    17761 gattatcgga cacagcgaag gcgcgattat tgcctcgatt gtggcagcgg gaaacgaggc
    17821 tggctatgcg agcacctact gtggagggca cgaaagtact tgtgacgcat gtggatcctc
    17881 tggattcaat gcgatctgcg aattttcaag acccaccttt atcgttctgc tcgcttgtcc
    17941 tggtgtacga ggttatgagc tgcttatcat gcagaatgct gcaatcggtc gagcctcagg
    18001 gctgagtgaa gaacagataa tcgaagcgaa caatctcaac cgcaggct
//
LOCUS       DAOSOB010000038        17988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3144_length_17988_cov_71.801985, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000038 DAOSOB010000000
VERSION     DAOSOB010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 17988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17988
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3144_length_17988_cov_71.801985"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /gene="lysA"
                     /locus_tag="PLS88_05385"
     CDS             complement(<1..1274)
                     /gene="lysA"
                     /locus_tag="PLS88_05385"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOU06808.1"
                     /translation="MMDMPFPPGILIDGSKCCFDGVDLGYLAHEFGTPLYVMSETIIR
                     ERCEELHRVFLSRYPGTRAYYASKALQALDVLKIIKEEGLGVDVVSGGELYAALKAGI
                     PPSDIIFHGNAKTDEELHYAISSGVGRIAVDNLEEIFRIEQEAKRLGRDQQVIFRVAP
                     GVDSHTHRFISTGSLDSKFGIPLEPSEAERYVNALALCPRVKFLGLHFHIGSQLLENT
                     SHLAALEIVLSFAKRLSKEFGLKLEELNFGGGFGIRYLPSDKAPALADFLDPMMEMVQ
                     EWSRKENLPMPVCAIEPGRWIVGEAGITLYKVIAVKEIPGVRTYVAVDGGMGDNIRPA
                     LYDARYHVETVSQAFSKLPGELEKEGNYSEAGAMKESHGDKDLRTWNSSKERTPRKVT
                     IAGRYCESGDIIVKDATLPDPMPGDILAVYSTG"
     gene            1372..2124
                     /locus_tag="PLS88_05390"
     CDS             1372..2124
                     /locus_tag="PLS88_05390"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOU06809.1"
                     /translation="MKKPIIGITSFIDRSRQSVPYFSLKESYVKAIEMAGGIPVILSA
                     TSPDVVNALIPTLDGLVFSGGGDIAPWLFGQEPLVGLGSFDTVRDEWEIELCNRAWEA
                     RIPMLGICRGCQLMNVARGGSLLQDIERSNPAALLHNPQIAHDELHHHIIIERDSILF
                     ELFETESLLVNSFHHQAVDKVASDFKITARGTDGIIEALEPIESGRFALALQFHPEGL
                     FLRYGAFLKPFSALIAASCKGDLTGESSLSHS"
     gene            complement(2191..3423)
                     /locus_tag="PLS88_05395"
     CDS             complement(2191..3423)
                     /locus_tag="PLS88_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOU06810.1"
                     /translation="MGIDMAPWNNPSFLQEVLLESKAMEEWLIEVRRSLHRIPEPGNK
                     EQKTSNLICKLLEEMNIPYRRSYGTAIVGLLEGKKPGHCVAFRADMDALPIEEPSDRP
                     YASLHHGYMHACGHDAHMTVALGVAKLLAAHRQDFSGSVKFLFQPAEETTGGAKPMIE
                     AGCLENPHVDLVLGLHVVPELPAGMAEVKIGPFYGASDSLEIVVKGRSSHAAYPEQGV
                     DAIVVSASIIQALQTIVSRSISALDNAVITIGKIQGGVRSNILANEVIMIGTIRTLSE
                     DLRCFMRTRVKEICTEIAAAFGASCEVHIRPSYPVLINDESATELVRATAISILGESN
                     VIMRQEPNLGVEDFAYFLRERPGTFWHLGCGKPRMEKIYPLHSAEFDIDEACLPIGVA
                     IQAASALTFLQFRNQEQG"
     gene            complement(3413..4381)
                     /gene="dapF"
                     /locus_tag="PLS88_05400"
     CDS             complement(3413..4381)
                     /gene="dapF"
                     /locus_tag="PLS88_05400"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOU06811.1"
                     /translation="MSVLPQGFHAHKYQALGNDYLVVDPAEVRISLSASAISALCDRN
                     RGVGSDGILYGPILSPSSLVVGSPFGLRIFNPDGSEAEKSGNGLRIFSLYLYERGLVA
                     DEPFQVQTKGGMVECRILATKDIYADDACSREEGYEEERDEDSEKHNEDLEERHEERH
                     IEVSMGEPRFLPGSSTLAIDGIEFRTVRVSMGNPHCVLLIQHPTEELARSIGPLVENH
                     PDFPNRTNVQFLEVIDRKTIRIEIWERGAGYTLASGSSSCAAAVAARRLGLVDDEVTV
                     KMPGGALTIDMREPNIRMTGPAVRSFDLVFSPYLMRLAMNMKENGD"
     gene            complement(4384..5508)
                     /gene="alr"
                     /locus_tag="PLS88_05405"
     CDS             complement(4384..5508)
                     /gene="alr"
                     /locus_tag="PLS88_05405"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOU06812.1"
                     /translation="MDHRATTAWVDLAAIRTNYRCLRDMAQGSIVCPVIKADAYGHGA
                     LAIAHELEREGAQYCSVALAKEALDLRESGIQMPILIFGAPEHSSSSLAIRLGCAQTI
                     YTEEHVRILASAAKSVGKKAKAHIKIDTGMNRQGLPWTKSRSFAGLLADYPEIFVEGI
                     YSHFADADNPDPSFTDLQMKRFSMALDSFAKVGIIPKYRHIANSAGMVLHPKTRLDMV
                     RPGILLYGLKPAPDMELPEGFEPALALTTGIVHIREVPAGESISYGRTFTTKRRSRIG
                     LLPIGYADGYPRALSNRASVLIHGVRAPVVGRVCMDMTMIDLTGIAGARVGDEVLLFG
                     GQALPVSEISSLLSTIDYEVVCMISKRVPRVHIDTKPQEE"
     gene            complement(5512..6948)
                     /locus_tag="PLS88_05410"
     CDS             complement(5512..6948)
                     /locus_tag="PLS88_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU06813.1"
                     /translation="MARAIKLYFLQSFLDKPNEALHGYIESAQYFFMEYNNFNGTYVL
                     EGVDLVLADSILPKAVLAVREGKIAAIIPSKPRKSSEKLKLSSQSSGKTSFSTPSQIE
                     LAYKIDPARQIDPAFENAPVIEAEGAFVLPSLMEMHIHGCGSWGFEKVSRPEDLFHIS
                     DFLETKGVGCYVPTILWEEKVLSELTKAILASGISRSRLPGIYLEGPFVNHSKRGGIM
                     PENILEPDPKLAEHIIDISKGLIKICAMAPELDGIDLIYPVFQNEAVLLSLGHSDAKL
                     SSIHVPPHPFSITHLFNAMTGIDHREGGLANFAFSQSPDYIELNGDGIHVNESCLKLC
                     SRLISENSLILISDAVIGAGLPYGDFSYYGRRVVSSKRGVRYADSDVLMGSNMLGIDI
                     VKNYCLEAEVPLWRAVRAMSLVPRKALGLDKEYGSIEVGKMADIFLWDKDLETAVRPK
                     ALLASENGFKKDDLLGNRFQVRRLQKKD"
     gene            7014..7832
                     /locus_tag="PLS88_05415"
     CDS             7014..7832
                     /locus_tag="PLS88_05415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU06814.1"
                     /translation="MTVPKNLNPSELRFIAMDMDGTILDEGYQLSPYVANTLKKLQNQ
                     GKKLIIATGRVYTAAKITLANSFEPDAFVCTNGADIFGPDGTAIAYHHIPVEALPILT
                     EVGRRHDVLFCCYIGEQWIYDRYSPLVDFYEKRTGMKGIQKDFDSLIGEDILKFLAIG
                     SNEELLEMREELANLAADLLDTVFSHETMLEIVARGVSKRQGLEECLRYLGGSLEETI
                     AFGDAENDLEMLKAVKVGVAMGNAHTSIQTAVPYVTASVDDDGVARFLEELFGI"
     gene            7939..8024
                     /locus_tag="PLS88_05420"
     tRNA            7939..8024
                     /locus_tag="PLS88_05420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7973..7975,aa:Leu,seq:taa)
     gene            complement(8097..8534)
                     /locus_tag="PLS88_05425"
     CDS             complement(8097..8534)
                     /locus_tag="PLS88_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU06815.1"
                     /translation="MIEVDQLQKYSLFGGISPEQIETIKPLLGTEHFEAGECPQVEGQ
                     PNDKIYFILSGEVEIIKKGLVIAHLKEGQTFGEMELLDIMPSIATVRATTALETVTIS
                     NRVLYDIFKLDLKAFSMMIMNLARDLSRRLRRMDDIVSSEGKY"
     gene            complement(8566..9519)
                     /locus_tag="PLS88_05430"
     CDS             complement(8566..9519)
                     /locus_tag="PLS88_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06816.1"
                     /translation="MTFEIFLQMFPIALMFSAPIILTALGGLFSERSGIVNIALEGIM
                     MVGAFTGATITVLLENSTPAAAWIGLLGGLLAGIVFSLLHAVASINLKADQVISGTAL
                     NILAGGLTVYLCQIIFHQQRTRAFSYGIKKLSVPGLSKIPVLGRMFFDQVYPTFYIAI
                     AMVLLTWFVVYKTPLGLRMRACGEHPQAAASMGINVSKMRYFGVLVSGALGGLGGAVM
                     VLTADIQYTLVSIHGTGFISLASLVFGRWNPFGVLGAGFFFGFSTALSFYAKDIPLLS
                     GLPGEFFYILPYLLTIIALLFFAGKSVGPKAAGQIYESGER"
     gene            complement(9516..10610)
                     /locus_tag="PLS88_05435"
     CDS             complement(9516..10610)
                     /locus_tag="PLS88_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06817.1"
                     /translation="MSTRKEKIVLPLIAVLLGFLLGSLIVILTGRSPISMYAAIIKGF
                     SGIDIINGQPINTRYIGEFIIQAMPIILTGLSFAFASRTGLFSIGAEGQLMIGSISAT
                     AVALLVQAPKAIHLPLVLLAAIAGGALWGAIPGFLKARYNVHEVVVTIMMNYIALHFN
                     NYVILNIFGSVDRVKTAPFPETALLKSPFLESITRNSRLNWGFIPVLIAVLIFSFIIN
                     KTTFGFSLRAVGYNKDAARYAGMKVNRNIIASMAIAGAFAGLAGGVITVGTFNFGRVI
                     QAAEGYGFDGIAVALVGGSEATGILLAGLLFGGLKAAQPLMQSQGIPREIAGIIQASI
                     VLFVAMKYGIKMVLDKFERKQVPTTEGERV"
     gene            complement(10629..12185)
                     /locus_tag="PLS88_05440"
     CDS             complement(10629..12185)
                     /locus_tag="PLS88_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06818.1"
                     /translation="MSNIVEMLHITKDFPGIRANDDITLQVEERSIHALLGENGAGKS
                     TLMSILFGLYQPDAGTIRIRGKEVKVSDPNVATALGIGMVHQHFKLVHNFTVTENIIL
                     GMEPRKGLLVDMRTAEQKVEDISKLYGLAVDPRSKIEEISVSMQQRVEILKMLYRDAE
                     ILIFDEPTAVLTPQEIHELMQIMRRLVEEGKTILLITHKLKEIKEVADVCSVLRRGKL
                     VGTVPVADTDEERMAEMMVGRQVSFHVQKTLAKPGDVLLRVEGLNVKNQKGVLGVKNL
                     SLSVRAGEILGLCGIDGNGQTELVQAITGLTKVESGRIFLRDIDITHLSIKERSDLGL
                     GHIPEDRQKHGLVLDFTLGENFIIHNYYKEPFAHWGILNKKAILENAQRLIKEFDVRS
                     GQGAITPARAMSGGNQQKAIVAREIDRSPDVFIVVQPTRGLDVGAIEYIHKRIIGERD
                     KNKAILLVSLELDEIMDLSDRIAVIYNGEIVGIVDAAETNENELGLMMSGALHKAQEA
                     AQTGREPAHV"
     gene            complement(12562..13566)
                     /locus_tag="PLS88_05445"
     CDS             complement(12562..13566)
                     /locus_tag="PLS88_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU06819.1"
                     /translation="MKKALLVLVVTAMIAVPAFAQFKVGLVTDVGGIDDRSFNQGTWE
                     GIVRFAQDYKLPKGNYKYLQSSAEADYVPNLSTFADEGLNLIIAPGFLFEQAMTQVAK
                     NYPTRKFLIIDTVVSLPNVVSATFAEHEGSFLVGVAAALKAKADGKNILGFIGGMQFP
                     LIEKFQAGFEQGVKAVYPDCKILVDYAGDFAAPDKGQALAQKQYNAGAYIIYHAAGGT
                     GNGLIKEAKERQQKGDNRWAIGVDKDQYADGIYSGNKSAVLTSMMKRVDVAAYEVSKM
                     TMEGKFPGGQVLVFSLKNKGVGLPENNPNLSADIIAKVKEYEALIASGKLVVSEIPGK
                     "
     gene            complement(13737..14420)
                     /gene="deoC"
                     /locus_tag="PLS88_05450"
     CDS             complement(13737..14420)
                     /gene="deoC"
                     /locus_tag="PLS88_05450"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOU06820.1"
                     /translation="MNKKWTSQMLAKVIDHTLLKATATEDQIKALCAEAKEFGFKAVC
                     INPYWVPLCARELADSDVLVATVVGFPLGANDSSIKAEEARLAVAQGADEIDMVINIG
                     KAKGGDWATVQDDITAVVHASKPAIVKAIIETCYLTQEEKISACRAAISAGATFVKTS
                     TGFGTGGATVEDISLMKKTIGEGAYVKASGGIKTYEDAVSMLEAGASRIGASAGVAII
                     REAETLENK"
     gene            complement(14457..15011)
                     /locus_tag="PLS88_05455"
     CDS             complement(14457..15011)
                     /locus_tag="PLS88_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06821.1"
                     /translation="MNKTAMLRLKRMHLAPYIQLATSLIGKPRRSGGNMFRHQLDTMA
                     ILIDYGYIDSVLLKASLIHDLLEDAPETNPDSILSIDDESIDVYKLVLEVTRRPVENK
                     AEFLIRIRDYGTPHARVLKSADRISNMISLGYVTDDVFVRRYTDETEQYIYPIAERAD
                     ARMLMELRELVATRREYLRNRIEI"
     gene            complement(15089..17746)
                     /locus_tag="PLS88_05460"
     CDS             complement(15089..17746)
                     /locus_tag="PLS88_05460"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOU06822.1"
                     /translation="MKSVELAKAYDPKSFEERIYEMWRLEGLFQPKIDKRKKPFTIVI
                     PPPNVTGALHLGHALDNCLQDIQIRYRRMKGIPTLWLPGTDHAGIATQNVVERKLRKE
                     GRNRETMGREAFIEETWKVASEHRAFIDNQLARIGSSLDWSRERFTLDEGLSKAVREV
                     FVRLYERDLIYRGEYLVNWCPSCGTALSDDEVEHEDEPGSLWYIWYELVDGPCPECPN
                     GKIEIATSRPETLLGDTAVAAHPDDKRYRSIIGKKVHLPLTERIIPIIEDSYVDPEFG
                     TGLVKITPAHDPNDFLVGQRHGLENINILNPDATLSDAVPEKYRGLNVLDARKAVLAD
                     LEAQSLLKSEEKIVHAIGHCYRCHSAVEPYLSRQWFVRMKPLADKALKAWQDGDVVFF
                     PKKWEHTYRHWLENIRDWCISRQLWWGHRIPVFYCIDCGTMMVCREDPTVCTHCGSSN
                     IYQDEDVLDTWFSSWLWPFSTLGWPEDTEDLRYFYPTSALVTGYDIIFFWVARMIMAG
                     MEFTGQSPFKEVYIHGLIRDKQGRKMSKSLGNGIDPLDIVEEYGADALKFTLAYNCAA
                     GQDILLDKESFKLGSKFANKVWNASRYILMNLEGRTLLEEHALAYSDTDRWIFHRLNE
                     AAKTASQAFESWRFNDAAQAVYSYFWNDFCDWYIEASKLSTKSGSESEKDRSTTMLLR
                     VLEESLRLLHPFLPFVTEEIYGMLPNATGRLIVASYPVWTKDRESPEIESQFKALQEI
                     VTLVRTLRSEFQIAPEISIPLSLEFDDSFSHGRFIQNHIELISLLAGASSVLIAKGPE
                     KGSVSLVGSGFTVHVQVRGLLDIAKLIDRLSREAERERAYIEKLKAKLGNQTFLSSAP
                     THIIEKEKEKLTLSMAKAAKLELYIEELS"
BASE COUNT         4573 a         4516 c         4109 g         4790 t
ORIGIN      
        1 ccagtcgaat agacggcaag gatatcgcct ggcataggat cgggcaatgt tgcatctttg
       61 actattatgt caccggactc acaatagcga cccgcaatcg tcacttttcg cggagttcgc
      121 tctttgctcg agttccatgt acgcaagtcc ttatcgccat ggctttcttt catagcgccg
      181 gcctcagaat aatttccttc cttttccagc tctccaggca gctttgagaa ggcttggctc
      241 acagtctcta catgatatcg agcatcataa agcgcaggcc ttatattgtc gcccataccg
      301 ccgtcgaccg cgacataggt ccgcacacca ggtatttcct tgacggcgat aaccttatag
      361 agcgttatcc ccgcttctcc cacaatccat cgccctggtt cgatggcgca gactggcata
      421 ggcaggtttt ctttccgact ccattcttgt accatctcca tcataggatc aagaaaatcc
      481 gcaagagcgg gcgccttatc ggaaggaaga tagcgaatcc caaaacctcc cccaaaattt
      541 agctcttcca gcttaagtcc gaattccttc gaaagccttt ttgcaaaact caacacaatt
      601 tcgagcgctg ccagatgcga ggtgttttcc aggagctgtg agccaatatg gaaatgcaag
      661 cccaaaaact tgacacgggg acagagagcg agcgcattca catatcgctc tgcttccgaa
      721 ggctcaagcg gaatgccaaa cttcgagtcg aggcttcctg tggaaatgaa tcgatgggtg
      781 tggctgtcta ctccaggagc aacgcgaaag attacctgct gatctctacc gagtctcttt
      841 gcttcctgct cgattctaaa gatctcctcg aggttgtcga cagcgatccg tcccactccg
      901 gagctaatgg cataatgtag ctcctcgtcg gtttttgcat ttccgtggaa tataatgtcg
      961 gatggaggaa ttcctgcctt caatgcagcg tagagctctc cccccgatac tacatcgact
     1021 ccaagacctt cttctttgat tattttgagc acatcgagag cctgcaatgc tttgcttgcg
     1081 taataggctc gggttcctgg atagcgggaa agaaaaaccc tgtgcagctc ttcgcagcgt
     1141 tcacggatga tagtctcgga catcacatat agcggagtgc caaactcatg agccaagtat
     1201 cctagatcga caccatcgaa gcagcatttg gagccatcga ttagaatgcc gggtgggaaa
     1261 ggcatatcca tcattgcttc ctccatctcc actttacttg cacttttcta gttattccag
     1321 cactttaggc ttgcactata ccttgcaaac ccgattggct acaataactg catgaaaaaa
     1381 cctattatcg gcattacgag ctttattgat cgctctagac aatcggtacc ttatttttct
     1441 ctcaaagaat cctatgtaaa agcaatagaa atggccggcg gcattcccgt gatcctctct
     1501 gccacgagcc cagatgtggt aaatgcactc attcctacac tagacggctt agtattttcg
     1561 ggtggaggcg atatcgcacc ttggcttttc ggccaggaac cgcttgtggg tcttggttcc
     1621 ttcgatactg tgcgcgacga atgggagatc gagctctgca atcgagcttg ggaagcaaga
     1681 atacccatgc tgggcatctg taggggttgt cagctcatga atgtcgcacg cggcggaagc
     1741 cttttgcagg atatcgagcg cagcaatcca gcggctctcc ttcacaatcc gcagatagcc
     1801 catgatgagc tacatcatca tatcataatc gaaagagata gcattctttt cgagcttttc
     1861 gagaccgaaa gcctcttggt aaatagtttc catcaccagg ctgtggacaa agtcgcgagc
     1921 gatttcaaga tcacagcgcg cggcacagat ggcataatcg aggcgcttga gcctatagaa
     1981 tctggacgat tcgctctcgc actgcaattc catccagaag ggcttttcct tcggtacggt
     2041 gcgttcctaa aacctttctc tgctttgata gcagccagct gcaaaggaga ccttaccgga
     2101 gagtcatctc tgagccattc ttgagcaatt tctgagcaat ttttgaatta ttctttagcc
     2161 attccttata aattcctagt tagacacttc ctagccctgt tcctgattgc ggaattgcag
     2221 aaaagttagc gctgaagccg cctgaatcgc gacacctatg ggaaggcatg cttcatctat
     2281 atcgaattcc gcggaatgaa gcggatagat tttttccatt cttggcttcc cacagcccag
     2341 atgccaaaaa gtccccggtc gctctctaag aaaatatgca aaatcctcga cccccagatt
     2401 gggctcttga cgcataatca cattggactc tccgagaatg gatatagcag tagcgcgtac
     2461 cagctccgtt gcgctttcgt cgtttatgag aacaggatag ctcggacgaa tatggacttc
     2521 gcaggaggca ccgaaagccg cggctatttc tgtacatatt tcttttaccc tcgttcgcat
     2581 aaagcatcgg agatcttccg aaagagtcct aattgtacct atcataatga cctcgtttgc
     2641 cagaatattg ctgcgcactc ctccttgaat cttgcctatt gtaattacgg cattatccag
     2701 agccgaaatc gagcgagaga ctatagtttg aagggcttga attatggatg ccgaaacgac
     2761 gatagcatcc acaccttgtt ctggataggc agcatgggag cttctgcctt tgaccacgat
     2821 ttccaagctg tcggaagctc catagaaggg accgatcttg acttctgcca tgccggcggg
     2881 aagttccgga acaacatgga gaccgagcac cagatcgaca tgcggatttt cgagacagcc
     2941 agcctcgatc atgggcttag caccgccggt agtctcttct gcgggctgga aaagaaattt
     3001 caccgatccg gagaaatcct gcctgtgagc tgcaagcaat ttcgctacgc cgagcgctac
     3061 tgtcatatga gcatcgtggc cgcaggcatg catgtagcca tggtggagcg aagcataggg
     3121 ccgatcggaa ggctcctcta taggtagagc atccatgtcc gcgcgaaaag caacgcagtg
     3181 cccaggtttt tttccttcca gaaggcctac aatggcagtt ccatagcttc ttctataagg
     3241 tatattcatt tcttcgagca gcttacagat gagattcgat gtcttttgtt ccttgttccc
     3301 tggttcagga atccggtgca gagacctgcg cacttctatg agccattctt ccatggcttt
     3361 agattctaaa agcacttctt gtaaaaacga tggattgttc catggcgcca tatcaatccc
     3421 cattctcttt catattcata gcaagacgca tcaaatacgg tgaaaataca aggtcaaatg
     3481 accgcacggc tggaccggtc attcttatat ttggctcgcg catatctata gttagcgctc
     3541 cgccaggcat tttcactgtt acctcatcat ctacgagccc gagtcttctg gctgccaccg
     3601 cagctgcaca ggaagacgag ccgcttgcga gagtataacc cgcacctcgt tcccagattt
     3661 ctattctgat cgtctttctg tcgatcactt ccaagaattg cacgttagtt cggttaggaa
     3721 aatcaggatg attctctact aaaggcccaa tactacgcgc tagctcttcg gtagggtgct
     3781 ggattaagag cacgcaatgc ggattgccca tcgataccct tactgtccga aactcaatgc
     3841 catcgatcgc aagcgtggaa ctgcctggca ggaatcgggg ctctcccatc gatacttcta
     3901 tatggcgctc ttcatggcgc tcttccaaat cttcattgtg cttttcgcta tcttcatcgc
     3961 gctcttcttc gtagccttct tctctagagc acgcgtcatc ggcgtatatg tccttggtgg
     4021 caagtattcg gcactcgacc atgccgccct tggtctgcac ttgaaaaggc tcatctgcca
     4081 caagtccgcg ctcatagaga tacagcgaaa atatccttag tccattgcca gacttttccg
     4141 cctccgagcc atcgggattg aatatgcgca gtccgaatgg actaccaact accaaggagg
     4201 atggcgatag aataggccca tataaaatac catcagagcc cactcctctg tttctgtcgc
     4261 ataaggcaga gattgcggaa gcagaaagcg atattcgaac ttctgccgga tcgacgacaa
     4321 gataatcgtt gcccaatgcc tggtatttgt gggcatgaaa accttgaggc aagacactca
     4381 tgatcattcc tcttgtggtt ttgtatcgat gtgaacgcgg ggcactcgct ttgagatcat
     4441 acataccact tcgtaatcga tagttgaaag cagactcgat atctccgaaa caggaagagc
     4501 ctgcccacca aatagcaaaa cttcgtcgcc aactcgtgct cctgctatgc cagtaagatc
     4561 tatcatggtc atgtccatgc atacacgacc cacgactggt gcgcggactc catgaatcaa
     4621 taccgaggca cgattcgaaa gggctctcgg atagccatct gcataaccta ttggcaagag
     4681 gccaattctg ctgcgacgct ttgtggtgaa agtccgacca tagcttatag attctccggc
     4741 gggcacctcc cttatatgta caatgccagt cgtaagcgcc aaggcaggct cgaaaccttc
     4801 cggaagctcc atatccggcg caggtttcag cccatagagc agaataccgg gccgcaccat
     4861 atcgagtctc gtttttggat gcagaaccat gccggcactg ttagcgatat ggcgatattt
     4921 cgggataatc cctaccttcg caaaactgtc gagcgccatg gaaaagcgct tcatctgaag
     4981 atcggtgaat gaaggatcgg gattatcggc atctgcaaag tgagagtata tgccttccac
     5041 gaatatttct gggtagtcgg caagcagccc ggcaaatgac ctggattttg tccatgggag
     5101 cccttgtcga ttcatgccag tatctatctt gatatgagct tttgctttct taccgacgct
     5161 tttggcggca gaagctaata tgcgcacatg ctcttcggta tagattgtct gagcgcagcc
     5221 tagtcgtata gcaagagagg atgatgaatg ctccggcgcg ccaaagatga gaataggcat
     5281 ttgaatgcca gactctctta aatcgagcgc ctcttttgcc agagcaaccg aacaatactg
     5341 cgctccttcg cgttcgagtt catgagctat tgcgagtgca ccatgtccgt aagcatctgc
     5401 ctttatcacc gggcagacaa tgcttccctg agccatatct ctaaggcagc ggtaatttgt
     5461 cctgatggcg gcaagatcga cccatgccgt cgtcgcacga tgatccatga ctcagtcctt
     5521 cttttgtaac cgacgtacct gaaatctgtt gccgagcaag tcgtctttct taaagccatt
     5581 ctcagaagcc agaagcgctt tcggtcttac ggcagtttcc aagtccttat cccaaagaaa
     5641 tatatcggcc attttgccaa cttcaattga accatattct ttatccagcc ctagcgcctt
     5701 gcgcggaacc aaagacatcg ctctgactgc gcgccatagc ggaacttccg cctctaaaca
     5761 ataattcttt actatgtcaa ttcctagcat attggagccc atgaggacat ccgaatctgc
     5821 atacctcacg cctcgcttag aggagacaac tctccgccca taatacgaga aatccccgta
     5881 tggaagccca gcccctatta cggcatccga aatcagtatt aacgaattct ccgaaatcaa
     5941 gcgagagcat agcttgagac agctttcgtt cacatgtatt ccatcgccat tcagttcaat
     6001 atagtccgga gattgcgaaa aagcaaaatt ggcaagccct ccttctctat gatcgatccc
     6061 tgtcatagca ttgaatagat gagttatcga aaaaggatga ggcggaacat gtatcgaaga
     6121 taatttcgca tcggaatggc caagggaaag aagcacagct tcgttttgga atactgggta
     6181 tattagatcg ataccatcca actcgggagc cattgcacag atcttaatca gccctttgga
     6241 tatgtcgata atatgctctg caagcttggg gtccggctct agtatatttt ctggcattat
     6301 gccgccgcgc ttcgaatgat ttacgaaagg accttcgaga tatatcccag gaagcctcga
     6361 tcgggaaatt ccgcttgcca ggattgcctt agtaagctcc gagagaactt tttcctccca
     6421 gagaatcgtc ggcacatagc accctacccc ttttgtctcg agaaaatccg atatatgaaa
     6481 gagatcttcc ggccttgaga ccttctcgaa cccccaagat ccacagccat gtatgtgcat
     6541 ctccattaaa gaaggaagca caaaagctcc ttctgcctcg attactggag cattctcaaa
     6601 tgctggatcg atctgacgcg ctggatcgat cttgtaagcc aattcaatct gagatggagt
     6661 ggaaaaactc gtttttccag atgattgaga agaaagtttt aatttttcgg atgatttgcg
     6721 cggcttggaa gggattattg ctgcaatttt accttctcga accgccagca cggcctttgg
     6781 caatatcgaa tccgcaagca caaggtcgac gccttccaga acataggtgc cattaaaatt
     6841 attgtactcc ataaaaaagt attgtgccga ttcgatgtag ccatgtaaag cctcgttcgg
     6901 tttatcaaga aaactctgga gaaaatacag cttgattgcc cttgccacaa aattgtagtc
     6961 gaccgcttat atcatatgat gaccaatttt gaactttcat atattatcgc cgcatgactg
     7021 tccccaaaaa cctaaaccca tccgagctaa gatttattgc aatggatatg gatggaacca
     7081 ttctcgatga aggatatcaa ctttctccct atgtggccaa tacactcaag aagcttcaaa
     7141 accaagggaa aaagctcatc atagcgacag ggcgcgtata tacagctgcc aaaataaccc
     7201 tcgcaaattc attcgagccc gatgcctttg tatgcacgaa tggagccgat atcttcggtc
     7261 ctgacggtac agcaatagct taccatcata ttccagtcga ggctctgcca atactcaccg
     7321 aagtgggaag acgccatgat gtgcttttct gttgctacat tggggaacaa tggatatatg
     7381 accgctattc gcctcttgta gatttttacg aaaagcgaac cggcatgaaa ggcattcaga
     7441 aggacttcga ctcactcata ggcgaagata ttttgaaatt tcttgctatt ggatcgaatg
     7501 aagagctttt agagatgcgc gaagagttgg caaacttagc cgccgacctt ctagacacgg
     7561 tattttctca tgaaactatg ctcgaaatcg ttgcaagggg tgtttcgaag cggcaaggtc
     7621 tcgaagaatg cctacgttat ttaggtggta gcctagaaga gacaattgca tttggcgatg
     7681 ccgaaaatga tctggagatg ctaaaagcgg taaaagttgg cgttgcaatg ggaaatgccc
     7741 atacctcgat tcagactgcg gtgccgtatg tgacggcctc tgtggacgac gacggtgtgg
     7801 ctcgttttct ggaagaactc tttggaatat gaaataggat tcgatcgatg cacgacaatt
     7861 ctataacaaa ggtaatcggc ttcttcgatt gtcttcttga ctgctttttg tgcgctagtt
     7921 aaaatctaga ttcaaattgc cggggtggtg gaataggtag acaccaggga cttaaaatcc
     7981 cttgctgcgc aagcggcgtg cgggttcgat gcccgtctcc ggcaattttt gattatttta
     8041 tgacactgct atcgctagaa aataattttt ggttttagga ttcttttcat cggatctcag
     8101 tattttcctt cgctagatac aatatcgtcc attcgccgca atctccgcga aaggtcgcgc
     8161 gcaagattca ttatcatcat agagaaggcc ttgagatcga gtttgaagat atcgtagagg
     8221 acgcgattag agatagtcac ggtttcaaga gcagtggtgg cgcgcacagt ggcgatgctc
     8281 ggcatgatat cgagaagctc catctcgccg aaggtttgcc cctctttgag atgagctatt
     8341 acaagcccct tttttattat ttcaacttca ccgctcagaa taaaatagat cttatcgttg
     8401 ggctgccctt ccacttgcgg acattctcca gcttcgaaat gctctgtgcc aagcagaggc
     8461 ttgatcgttt cgatctgttc gggactgatg cccccaaaaa gcgagtattt ttgaagctgg
     8521 tcgacttcga tcatttttgc ttccttcaag attattatgc ccttctcaac gttcccctga
     8581 ttcgtagatc tgtccggctg ccttgggtcc taccgatttg cctgcaaaga atagaagggc
     8641 gatgatggtg agcaaatagg gcagaatata aaagaattct cctgggagcc ctgaaagcag
     8701 cggaatatcc ttcgcataaa aagatagggc tgtagaaaac ccgaaaaaga atccggcgcc
     8761 gagcactccg aagggattcc atctaccgaa taccagcgaa gcaagggaaa taaagcctgt
     8821 gccatgaata gacacaaggg tatactggat atccgccgtc agcaccatta cggctccgcc
     8881 gagtccgccc agagcccctg aaaccagaac gccgaaatag cgcattttgg aaacattgat
     8941 acccatgctc gcggctgctt gaggatgctc gccgcaagct ctcatgcgaa ggccgagtgg
     9001 tgtcttgtac accacaaacc atgtcagcag caccatggca atggcaatgt aaaatgtagg
     9061 gtacacctga tcgaaaaaca tcctcccaag cacaggaatc ttgctcaatc ctgggacgct
     9121 caacttcttt atgccataag aaaatgcgcg agtcctctgt tgatggaaaa tgatctggca
     9181 gaggtagacc gtaagccctc cggctaaaat attgagggct gtacctgaaa tgacttgatc
     9241 ggccttgaga ttgatgctcg caacagcatg gagcagactg aagactattc cagcaaggag
     9301 tcctccaagt aggccaatcc atgccgccgc aggagtcgaa ttctccaaga gaactgttat
     9361 cgttgcgcca gtaaaggcgc ctaccatcat tattccttcc aaggcaatgt tgacgatacc
     9421 cgatcgttcg ctgaagagtc ccccaagggc tgtcaagata attggcgccg aaaacataag
     9481 cgcaatagga aacatctgaa gaaagatctc gaaggtcata cgcgttcccc ttccgttgta
     9541 ggcacctgct ttcgctcgaa tttatcgagc accattttga tgccatattt cattgcaacg
     9601 aatagcacaa tcgaggcctg aattattcca gctatctctc tgggtatgcc ttgagactgc
     9661 attagaggct gggcagcctt gagcccaccg aacaaaagac ctgcaagcaa gattccggtt
     9721 gcctcactcc ctccgaccag agccacggcg attccatcga aaccatagcc ttccgccgcc
     9781 tgaattacgc gaccaaaatt gaaggttcct acagtgataa ccccacccgc aagaccggca
     9841 aaagcgcctg cgatcgccat agatgctatg atatttcggt ttactttcat gccagcatag
     9901 cgagccgcat ccttattgta gcccacggca cgcaatgaaa aaccaaaggt agtcttattt
     9961 atgataaaag aaaatatcag aaccgcaatt agaactggaa tgaaacccca attgagccga
    10021 gagtttcgag ttatgctctc cagaaatggc gacttgagaa gagcggtttc ggggaagggc
    10081 gcggtcttta ccctatccac acttccaaaa atgttaagaa ttacataatt attgaaatgc
    10141 agagcaatgt aattcatcat tatggtaacg acgacttcgt gcacgttgta tcgcgccttc
    10201 aaaaagccag gaatggcacc ccaaagcgct ccgcccgcaa tagccgccaa caacacaagt
    10261 ggcaggtgaa tcgccttcgg tgcttgcacg agaagcgcaa ctgctgtagc cgagatcgag
    10321 cctatcatca gctggccttc ggcgccgatg ctaaagaggc cagtacgcga agcaaaggca
    10381 aaagataaac ccgtgagaat aatcggcatt gcctggatta tgaattcacc aatatagcga
    10441 gtgttgatcg gttgtccatt tattatgtct atgcctgaga atccctttat tatggcagca
    10501 tacatagaaa ttggagaacg accagttagt atgacgatca acgaacccag caagaatcca
    10561 agtagcacag caataagcgg aagcactatc ttttcttttc gagtagacat cggtcttcct
    10621 cccttccctt agacatgcgc tggttctcgg cctgtctggg cggcttcctg tgctttgtga
    10681 agtgcccctg acatcataag accgagttcg ttctcattgg tctcagctgc atcgacgatg
    10741 ccgacgatct ctccattgta gataacagcg atgcgatcag agagatccat gatctcatcg
    10801 agctcgagag agaccaagag aatagctttg tttttatcgc gttctccaat gatacgcttg
    10861 tgtatatatt cgatcgcccc tacatcgagg cctcttgtcg gttgaaccac gataaataca
    10921 tcgggcgaac ggtcgatttc gcgagccaca atggctttct gctggtttcc tccagacata
    10981 gctcgagctg gcgtaatggc tccctgcccg gaacgcacgt cgaattcctt gatgagcctt
    11041 tgtgcattct ccagaattgc ttttttgttc agtattcccc aatgcgcgaa aggctctttg
    11101 tagtaattgt gaattatgaa attctcgccc aaagtgaagt ctaggacgag cccatgcttc
    11161 tggcgatctt ctggaatatg cccaaggcca agatcagacc tttcctttat ggaaaggtga
    11221 gtgatatcga tatctcgaag gaaaatcctg cccgattcta ccttagtcaa accagttatc
    11281 gcttgaacga gctctgtctg accattgcca tctataccac agaggccgag gatttcgccg
    11341 gcgcgcaccg agagcgatag attcttgacg ccaagcacac ctttttgatt cttcacattg
    11401 agcccttcga cgcgcagaag tacatcgccg ggctttgcta aagttttctg tacatgaaag
    11461 ctgacctgac ggccgaccat catctcggcc attctctctt catccgtgtc tgcaacggga
    11521 acggtaccta caagcttccc tcttctcaga accgagcata catcggcgac ttcttttatc
    11581 tctttgagtt tgtgagtgat aaggaggatg gttttgcctt cttcgaccaa tcgcctcatt
    11641 atctgcatga gctcgtgaat ttcctgcgga gtaagaaccg cggtaggctc gtcgaagatt
    11701 aggatttcgg catcccgata gagcattttg agtatttcca cgcgctgctg catactcacc
    11761 gagatttctt ctatcttaga tctcggatcg actgcgaggc catagagctt cgagatgtct
    11821 tcgacttttt gctctgctgt gcgcatgtcg acaagaaggc cttttctagg ctccatgccg
    11881 agtattatgt tttctgtcac ggtaaagtta tgaactagct tgaaatgctg gtgaaccata
    11941 cctataccga gagcggtcgc aacattgggg tcgcttacct ttacctcttt gccgcgaata
    12001 cggatcgttc ctgcatcggg ctgatatagc ccaaaaagga tgctcatcag tgtagacttc
    12061 ccagcaccat tctctcccag aagtgcatga atcgagcgct cttcgacctg aagcgtgata
    12121 tcgtcatttg ctctgattcc ggggaaatcc ttggtgatat gaagcatctc tacgatgtta
    12181 gacacctata aaccccctgg attaagatga agtaagatta tatcacagcg agattattct
    12241 cgctatataa aaaagaaata cgcttagcta tctttgccac aaaattgtcg tgcacccaga
    12301 ctaacacaag tcgtattcaa ctaggaaatg atagacagtt cagtatatca gaatatgcaa
    12361 atcatccaaa aatcggtctt gaaatggtat ttctatagaa tcgcgatatc ttaaatgcaa
    12421 taaaaaagcc ccctttttgg gggcttttga aacaatagag cagaggaagc aatccgcaca
    12481 aagctggttt tttaagcatt ttaggctcta gcaaattgct gattcgggcg cttcctcttc
    12541 ttccaaagtc tgctttttag cttatttgcc tggaatctcc gagacaacaa gcttgccaga
    12601 tgcgataagt gcctcgtatt ccttgacttt tgctatgatg tcagcggaga ggtttggatt
    12661 gttctcagga aggccaacgc ccttgttctt caaggagaag acaaggacct gtccgcctgg
    12721 gaatttgcct tccatagtca tcttgctgac ttcataggct gcgacatcga ctctcttcat
    12781 catcgaggta aggactgcgg atttgttacc actatagatg ccatcggcgt attgatcctt
    12841 gtcgacgccg attgcccagc ggttatcgcc tttctgctgg cgctcttttg cttccttgat
    12901 aagtccgtta ccagtaccgc cggctgcgtg atagattatg taggctccgg cattatactg
    12961 cttttgagcc aaggcctgac ctttatcggg ggccgcaaag tcacctgcat agtccacgag
    13021 gatcttgcaa tctggataga cagccttgac accctgctcg aatcctgcct ggaacttttc
    13081 gattagcggg aactgcatgc cgccaatgaa tccaaggata ttcttgccat cagccttggc
    13141 tttgagagcc gctgccacgc ccactaggaa agaaccttca tgctccgcaa aggtagcgga
    13201 tacaacgttt gggagagaga cgactgtatc gatgatgagg aacttgcggg tggggtaatt
    13261 ctttgcgacc tgggtcatcg cttgctcgaa caggaatcca ggggcgatga tgagattgag
    13321 gccttcatcg gcaaaagtcg ataggttggg gacataatcc gcttccgcgc tggactgcag
    13381 atacttgtag ttgccttttg gaagtttgta atcctgggca aagcgaacaa tgccttccca
    13441 tgtaccctgg ttaaaggacc tgtcgtcgat accaccaacg tcggtcacga gccccacttt
    13501 gaactgagca aatgctggta ccgcgatcat agcggtgacg acaagcacca aaagcgcttt
    13561 tttcatgctt catcctcctt attataggtt cgatgctata tatgcgtaga ttctatgcca
    13621 atctatcgca taaaatcaga taaatggtaa agccgaggca gcggattgtc aataacaatt
    13681 agctaccttt gcaaagattt gtattacaca cctttgcaat atgatggcta ttccaactat
    13741 ttattctcta atgtttcagc ctcccgaatt attgccacgc ctgcggaagc tccgatacgc
    13801 gaagcgccag cctctagcat tgatactgca tcctcataag tcttaattcc gcccgaggcc
    13861 tttacatagg ctccttcgcc tatggttttc ttcataaggc tgatgtcttc tacggttgca
    13921 ccaccggtgc caaagccggt gctggtcttg acgaaggttg ctcctgcgga aattgctgct
    13981 ctgcatgcgg aaatcttttc ctcctgtgtc agataacagg tctctattat ggccttgact
    14041 atggctggtt tagatgcatg tacaactgcc gttatatcgt cctgaaccgt agcccagtct
    14101 ccgcctttgg ctttgccaat gtttattacc atgtcgatct catcggctcc ctgggcaacc
    14161 gccagacgtg cttcttcggc ttttatggaa ctatcgttag caccaagcgg gaaaccaacg
    14221 actgttgcaa cgagaacatc tgaatctgcg agctctcttg cacatagcgg aacccaataa
    14281 ggattgatac agactgcttt gaatccgaac tcctttgctt ctgcacagag cgcttttatc
    14341 tgatcttcgg ttgcggtagc cttgagaagc gtatgatcga taactttagc aagcatttgg
    14401 cttgtccact tcttattcat gttgcttttc ctccgccttc acaggcgctg tctttgctat
    14461 atttcaattc tatttcgcaa atattcgcga cgcgtcgcca caagttctct caattccatc
    14521 agcatgcgcg catccgcgcg ttcagcgata ggatagatgt attgttctgt ttcatcggta
    14581 tatcgcctta cgaatacatc atctgttaca taaccaagac tgatcatgtt ggaaatacga
    14641 tccgcgctct tcagcactct tgcgtgcggc gttccgtaat cgcgaattct aataagaaac
    14701 tcggccttgt tctcgacagg cctccttgta acttcgagga ccaatttata tacatctatg
    14761 gactcgtcat cgatagaaag aatggaatcc ggatttgttt ccggagcgtc ttctagtaga
    14821 tcgtggatta gagaagcctt taagagcacg gaatcgatat aaccatagtc gattaggatg
    14881 gccatagtat ccagttggtg cctgaacatg ttgcccccgc ttctcctggg ttttccaatt
    14941 agagatgtag caagctgaat atacggcgcc agatgcatgc gttttaggcg caacatagcc
    15001 gtcttattca tagtcaggcg ctcggcacga agcgcagaat ttagcctcat aataggaatg
    15061 tctacttgag ggctactctc atccggactc atgagagctc ctcgatatat agctcgagct
    15121 ttgcagcctt tgccatggaa agcgtgagtt tttccttttc tttttcgatg atatgagtag
    15181 gggcagagga aagaaatgtc tgattgccaa gttttgcttt gagcttttcg atgtatgctc
    15241 tctctctttc ggcttctctg gaaaggcgat caatcagttt tgcgatatcg agaagacctc
    15301 gtacctgcac gtggacagta aatccactgc ctaccaagga tacactcccc ttctctgggc
    15361 ccttagcgat caataccgag gaagcacctg ccaataaact tatgagctct atatgattct
    15421 gaataaatcg accatgcgag aaactgtcgt caaactcaag agatagagga atcgagatct
    15481 cgggcgcaat ttgaaactcg cttctaagtg tacgcaccaa tgtcacaatc tcttgaagag
    15541 ctttgaattg tgattcgatt tcgggcgatt ctcgatcttt tgtccatacg ggataggatg
    15601 caacaattag gcgtcctgtg gcatttggta gcatgccata gatttcttct gttacaaagg
    15661 gaaggaacgg atgcaaaagc ctaagcgatt cttccagtac tctgaggagc atcgtagtag
    15721 accgatcctt ctccgattca cttccagatt tggtcgacaa ctttgaagct tcgatatacc
    15781 aatcgcagaa gtcattccaa aaataggaat agactgcctg ggcggcgtca ttgaagcgcc
    15841 aggattcgaa agcctgacta gctgtttttg cggcttcatt cagcctatgg aaaatccatc
    15901 gatcggtgtc gctataggca agagcatgtt cctcaagaag agtacggcct tcgagattca
    15961 taaggatata gcgcgaggca ttccatacct tatttgcgaa tttgctgcct aatttgaagc
    16021 tttctttatc gaggagaata tcctgtccag cagcgcaatt ataagctagg gtaaatttta
    16081 gagcatctgc accatattct tcgactatat cgagtggatc gatgccattc ccgaggcttt
    16141 tggacatctt gcggccttgc ttgtctcgga ttagaccatg aatgtagact tccttgaagg
    16201 gactctgccc tgtaaactcc atgccagcca ttatcatcct ggctacccag aaaaatatga
    16261 tatcgtaacc agttaccaag gcacttgtag ggtagaaata tcttaggtct tccgtatcct
    16321 caggccagcc aagcgtactg aaaggccaaa gccaacttga aaaccatgtg tctaggacat
    16381 cttcgtcctg atagatattg ctggagccac agtgtgtgca taccgttggg tcttcgcggc
    16441 agaccatcat ggtgccgcaa tcgatgcaat agaacactgg tatgcggtgc ccccaccaaa
    16501 gttggcgaga aatacaccag tcgcgaatat tttcgagcca atggcgatag gtgtgctccc
    16561 actttttcgg gaaaaagaca acgtcaccat cttgccaagc ctttagagcc ttatctgcga
    16621 ggggtttcat tcgtacaaac cattgcctag agagatatgg ttctacggcg ctatggcatc
    16681 tgtagcagtg accgatcgca tgcacgattt tttcttcgct ttttagaagg ctctgtgcct
    16741 cgaggtctgc aagcaccgct ttgcgggcat cgagtacatt tagaccgcga tatttctctg
    16801 gaacggcatc cgatagagtc gcatccggat tgagaatatt gatattctcg agtccatgac
    16861 gctggcctac tagaaaatca ttcggatcat gggctggtgt tattttgacc aggcctgtac
    16921 cgaattccgg atccacatag ctgtcttcaa ttatggggat tatgcgctcc gtaaggggta
    16981 aatgaacttt cttgccgatt atgctacgat agcgcttgtc gtcgggatga gccgcaacag
    17041 cagtatcgcc cagcagtgtc tctggacgag aggtcgcaat ttcgatcttg ccattagggc
    17101 actctggaca aggcccatcc acaagttcgt accagatgta ccatagagaa cctggttcat
    17161 cctcgtgttc cacctcgtcg tccgaaagag cagtaccgca ggacggacac cagtttacaa
    17221 ggtattcgcc ccgatatatc agatctcttt cgtagagcct aacaaagact tctcggacgg
    17281 ctttcgacaa tccttcatcc aaagtgaatc tttccctgct ccagtcgaga ctggatccaa
    17341 ttctagcaag ttgattatcg atgaaggcac gatgctcact cgcaactttc catgtctctt
    17401 cgatgaaggc ttcacgtccc attgtctcgc ggtttcgccc ttcttttcta agtttgcgtt
    17461 ccactacatt ctgggttgct atgcctgcat gatctgtacc aggcagccaa agcgtcggaa
    17521 ttcctttcat gcgacgatac ctaatctgga tatcctgtaa gcagttatca agtgcatgcc
    17581 ccaaatgtag agcacctgtt acattgggag gaggaattac tattgtaaag ggctttttcc
    17641 gcttgtcgat ttttggctga aaaaggcctt cgagcctcca catttcgtag atgcgctctt
    17701 cgaacgattt ggggtcgtaa gcttttgcaa gctcgactga tttcatgctc tcctccgcat
    17761 tgcacttatt ccatcgatat catcgaatta ttggaagtgc acaaaagaat accccaaaac
    17821 caaggccgcg gggaaggggg caaaatgatt tttcaacctc ttttcaacgc catttttctg
    17881 tatcataata gcagctatga gagaaacaga accatggaaa ttcctcgatg catatcgagg
    17941 tcgcgttttt tcggacagat ggcctactat tccacaacta ttcgacat
//
LOCUS       DAOSOB010000039        17518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3262_length_17518_cov_85.425242, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000039 DAOSOB010000000
VERSION     DAOSOB010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 17518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17518
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3262_length_17518_cov_85.425242"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /gene="pgsB"
                     /locus_tag="PLS88_05465"
     CDS             complement(<1..1465)
                     /gene="pgsB"
                     /locus_tag="PLS88_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HOU06823.1"
                     /translation="MISLVIIVSISLAMAAIVEYQAHCRRLKAVPIRILVNGTRGKST
                     VTRLIAAGLAAGGLRVYAKTTGSAARIILPDLSEREIRKHGSAKRRPSIMEHKWFVRA
                     AYKAGAQAIVAECMAIRPETIGVLETRLAHSTIGVITNVRLDHLDTMGTELESVAEAL
                     GESIPERGKAFVGSEGMDEEIKAVFSRMAAQRGAHLAFVPIDEETQALRSAFAYPIHA
                     QNLALALAICSACGVSREIALRGMQGAKPDPGVRSTILTSWRIHKVRVINAFAANDPQ
                     STLEMWHEERGLRYSSDLCYPSDMRNLNELDSNYGPQENETHEILSEIKEFNSAEQTF
                     RVLVFNHREDRAWRALQMGEIAAGIDADAILVYGLNRRLAEKVVHRYYRRLSAESPRA
                     LRAQRMQTAAQPAPLWLAEPPEEPVQSAMTAAPKAPTREAPQPSPHQAQLGLPLSKSQ
                     NKPASHPIKYPAVRSMRQASFDAIFETIENLGGFKEFP"
     gene            complement(1537..2022)
                     /locus_tag="PLS88_05470"
     CDS             complement(1537..2022)
                     /locus_tag="PLS88_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06824.1"
                     /translation="MDTVYLSKDLALQQVSSFLLRWKHNTDQSIDLDMTKGFETSEDA
                     SRLLRKVPSSVQKIGCYYVASQKKAIVWLITNESSDDRSDKERRQDSLQILLGKPREF
                     GSAPVEIRYNVMTNSADHHNGACLSGIEISYGSILILSPPAAGEIVISISRLEQAREL
                     A"
     gene            complement(2070..3158)
                     /locus_tag="PLS88_05475"
     CDS             complement(2070..3158)
                     /locus_tag="PLS88_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06825.1"
                     /translation="MKKLFLVWFVLIGLFVSQAQTESGVALVMSEVKAGKGVEKILSL
                     SDFSSQVKGTPFDTPVFFLGDIAGLLTKNAPEGFLSGKVAERQESTSKDYTVAIIAGA
                     HANEIAGTLAAYWIIEHCRVSGGTLIVVPRANASAATWSISNAAAPILHYGARLSNLA
                     HENGPDPDFFIPASALESFPALSGQEVRNLNRRYPGDPMGNRTSQTAYAITQLLMQNH
                     VNAALDLHEASPGSSLAWSIITRPEYLDIAALAVLQIEEETGKSFHLESSREEFAGYS
                     HWEWGKLGIRAFLVETLNPAQPSDDPTVDQIYNDKAPLAERIYIHLCTLKALVASAYE
                     ELGDLVALNIEGLPSSKEEVQKWLLGME"
     gene            complement(3155..3697)
                     /locus_tag="PLS88_05480"
     CDS             complement(3155..3697)
                     /locus_tag="PLS88_05480"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOU06826.1"
                     /translation="MALSILPIFAQYSVPSNLVANFERPGFITSAGQTSDAAIVKVLA
                     NTRLKLGLDYDIAAKPQAIENAKTLVLVLGVSNKGLGAAGLSLEQEIERVKNIVAEAL
                     KNNTRIISIHTGGTARRGEASNQVIELCVPVSNVVIVVEGGNNDGFFTSLCEKNNVPL
                     VQVPSIAEAGNVLMSLFAAQ"
     gene            complement(3760..5430)
                     /gene="ggt"
                     /locus_tag="PLS88_05485"
     CDS             complement(3760..5430)
                     /gene="ggt"
                     /locus_tag="PLS88_05485"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOU06827.1"
                     /translation="MVQKNKNVGLAMKHGAKNFLLFLLAAFVFALPLSAADTIKAEIT
                     ASNGMVASAQPLASAAGLEILMAGGNAVDAAIASAFALGVVEPNATGLGGEGMMVIYL
                     AENKTTIAIDYRSMAPLADMSKIKFGSEGHVAVAVPGTVAGLCTALKDYGTKSLAEVM
                     APAIRYARNGFIVSETLAQTIADRFDPISRNESLLQILAPEGLPLQTGDIFKNPDLAV
                     TLEKIAAGGPDVFYKGEIAEAIAKDMAKNGGFITKADLAAYRAIKREPVRGTYRGYEI
                     VSAPPPVGGISVIEMLNMLECFDLGSEQPLSPRNVHIMSEVMKRGFADNSAYVGDPDF
                     TQIPVKGLLDKEYARSRAQEIDLNKITTSIKAGTPNEHPSTTHLSVVDRHGNMVALTQ
                     TLSGFWGACVAVPGTGIILNNEMQNWSSRGPNAYAPGKRMRTTIAPTIIAKDGIPFVT
                     MGTPGAGRIISTMVILTVNLIDFDMGVQEAIESPRFYARDTEKDLSIESRIPKETQEW
                     LKSIGYSIKEYPPFDLFFGGAQAILVDPKTGIMHGGADPRRDGAVFGF"
     gene            complement(5459..6511)
                     /locus_tag="PLS88_05490"
     CDS             complement(5459..6511)
                     /locus_tag="PLS88_05490"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOU06828.1"
                     /translation="MRRSVFLLLICIFSCLILSPICSDPSLGANTILHDIRPGKGITQ
                     ILWLSEWFAPLKGTPSDTRVFIADSKKPGATVFVAGGTHANEIAGIMSAVILVENAIP
                     SYGRLIVIPDINMSASTWTESTIVPSWIRIDSPHGARFFKYGARYTDPVHQGMTDPDR
                     YKHPKGGDSFEGSESRNLNRVYPGKPDGTLTEQLAWAVMNLLKNEKVDIAFDLHEAGP
                     ESRLANMIVANPKNLDLGALAVINLELEGINMKLEPSSDVFHGLSHREWGDETNAFAF
                     LIETPNPAQATDKGNPVEDSKFPLEERVATHLATIEAILDAWNSDSLPDKHIEFSMFP
                     NWQDIKEQGIGKILNW"
     gene            complement(6647..7849)
                     /locus_tag="PLS88_05495"
     CDS             complement(6647..7849)
                     /locus_tag="PLS88_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOU06829.1"
                     /translation="MKKLVAVILALAFLVVGADVFAQAKAKFHIGIATETVSQAEDDL
                     RGAEQLIKEYGSVKDGGMIQHITYPDDFMSQQETYISNVVALADDPLMKAIVINQAVP
                     GAAEAFKRVRAKRPDILLLAGEPHEDPLVIQQAADLAVSNDFVSRGYTIIWAAKQLGA
                     KNFVHISFPRHMSYETLGRRRAIMEEACKDLGINFYFETAPDPTSDVGVAGAQQFILE
                     KVPQWVQKYGKDTAFFCTNDAHTEPLLKQLLAYGGYFVEADLPSPLMGYPGALGLDLS
                     KEAGDFPAILKKVEKAVIDKGGAGRFGTWAFSYGYTVTAGLGEFAKRVIEGNAKKDSL
                     KDVFDAFGKYTPGAKWNGAYYTDMATGVRARNQVLIYMDTYIFGKGFLPTTQVKVPDK
                     YYMIKMHN"
     gene            complement(7989..8516)
                     /locus_tag="PLS88_05500"
     CDS             complement(7989..8516)
                     /locus_tag="PLS88_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06830.1"
                     /translation="MNGSTSEKNGQNAQNVKKRRLIIRAALLAVYVLLIVLMIHSGRR
                     HTILIDNKDPHDGSYSAIDGMEVSIDNQEPLEYYRGDRDKAVVQGQKHIIKVEIFDDG
                     RKIEKSFAVPLWTDMVIIYVPKLVNGIEPWLEPFTVTEQLEESQESISPDEEISFQSM
                     GSPQLEDLGKASPTP"
     gene            complement(8513..9655)
                     /locus_tag="PLS88_05505"
     CDS             complement(8513..9655)
                     /locus_tag="PLS88_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06831.1"
                     /translation="MSAKKKFSTFLSRYAVVIIFLIITIAATPPSGLSVRYILQELIT
                     RLGRNSFLVLALLLPIYAGMGLNFGMTLGAMAGQIGLIFAVNYSIAGWQGLVFALLVG
                     LPISVLLGWICGLVMNKAKGREMVTGYILAFFMNGIYQFFVMYMLGSVVPMGNKAIVL
                     SRGYGIRNTLNLESVRQSLDNILVLRIAGFSIPVVTFIVIAVLCVFIVWFKKTKLGQD
                     MRAVGQDMAVAESAGIPVEKTRIIAIIISTVLACAGQIIFLQNMGNLATYNAHDQTGF
                     FAVASILVGGASVTSANIANVFIGVMLLHAMFVVSPLAGQNLFGSAMIGEYFRQFIGY
                     GVIALSLVLYAWRSKKAAAEARLGLRGVNNSHNNKVEITTKNGGQA"
     gene            complement(9652..10695)
                     /locus_tag="PLS88_05510"
     CDS             complement(9652..10695)
                     /locus_tag="PLS88_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06832.1"
                     /translation="MRNIKTFIQDFGWPRIIIFLFLVGLFIMAPFVQVRLDASISDVL
                     NRFGQNAIMVLAMVPMIQSGCGLNFGLALGLVSGLLGATLSLQLELHGMIGFLSAIVL
                     AMPFSILFGWIYGKLLNRVKGEEMTIAMYVGFSFIMFMCIMWLVLPYDNPTMVWGYAG
                     KGLRTTISVENYWLHVLNNFLAIKIGEFFIFPTGLLLFVALFAFFMWVFLRTKTGTAM
                     TAVGSNPDFARASGVDVDKMRTISVIVSTFLGAVGIIVYEQSFGFIQLYTAPNAMVFP
                     AVAAILIGGASINKASMVNVVVGTILFQGLLTMTPSVINSLLHTDMSEVIRIIVSNGM
                     ILYALTRKMKVSK"
     gene            complement(10688..12289)
                     /locus_tag="PLS88_05515"
     CDS             complement(10688..12289)
                     /locus_tag="PLS88_05515"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU06833.1"
                     /translation="MINGSSLLSIEHVSKDFYGNIVLHDVSFDLHEGEILGLVGENGA
                     GKTTLMHILFSMPVIAETGGFQGSIKIEGTEVHFESPFDALDAGLGMVHQEFSLIPGF
                     TTTENIVLNREPMRPTVLNDVFGDRMSVLLRDKMRERAEKAINKLGVNLDPDMLVSEM
                     PVGHKQFTEIAREIDREQTRILILDEPTAVLTESEAEVLLQTLKRLASEGIGIIFISH
                     RLQEVLSIADRIVVLRDGHLVKDTPNQNINLRDVASWMVGRTIAGEEDRYQLRSFVDI
                     ALNVRNLWVDMPGETVRDVSFDVHKGEIFGIGGLAGQGKLGIPNGIMGLFPAGGEVLL
                     DGKPVPLNNPAMALAMGMGFVSEDRRGVGLLLDESLEWNIAFTALQIHNQCLKRMLAG
                     LFTQRDEKAMEELCQEYVKLLDIKCTSTKQKAKELSGGNQQKICLAKAFAIKPQLLFV
                     SEPTRGIDVGAKKLVLDALHHYNRDFGTTIVMVSSELIELRSICDRIAIVDEGRIAGI
                     LPPTADISEFGLLMSGAAMEEAVNA"
     gene            complement(12436..13638)
                     /locus_tag="PLS88_05520"
     CDS             complement(12436..13638)
                     /locus_tag="PLS88_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOU06834.1"
                     /translation="MKKLVAVILALAFLVVGADVFAQAKAKFHIGIATETVSQAEDDL
                     RGAEQLIKEYGSVKDGGMIQHITYPDDFMSQQETYISNVVALADDPLMKAIVINQAVP
                     GAAEAFKRVRAKRPDILLLAGEPHEDPLVIQQAADLAVSNDFVSRGYTIIWAAKQLGA
                     KNFVHISFPRHMSYETLGRRRAIMEEACKDLGINFYFETAPDPTSDVGVAGAQQFILE
                     KVPQWVQKYGKDTAFFCTNDAHTEPLLKQLLAYGGYFVEADLPSPLMGYPGALGLDLS
                     KEAGDFPAILKKVEKAVIDKGGAGRFGTWAFSYGYTVTAGLGEFAKRVIEGNAKKDSL
                     KDVFDAFGKYTPGAKWNGAYYTDMATGVRARNQVLIYMDTYIFGKGFLPTTQVKVPDK
                     YYNIQMHK"
     gene            13785..>14993
                     /locus_tag="PLS88_05525"
     CDS             13785..>14993
                     /locus_tag="PLS88_05525"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU06835.1"
                     /translation="MQFSWKRFADELYYYSNQFILFFVLIISLTSGSWKNALVTSLIV
                     LSFMLIQTLLLTIQGHIPILRFIYSFITPAGYSILRATLSDIAFTETVNLLLWGASVY
                     IAIFQTLTLILRKPILKRFSEAALSLGSAIIFLLFYAYLDLRISVTASFVKGEIDAEA
                     MAQALGIRSFFAVFNKFFQAPQHLFALFGVISFDFMLMVARWRAINLEQRLTRIIEMP
                     QEAKRIYSSSPESEKGASPLEYEGQEVQPVLTTETQDSLPTKTIASLFVTAISSDIIG
                     FTDLSEQLGHKEATALLNRYYAIWTRCAGSYGGRIVSISADTVVILFGLVDQERSADR
                     ALNASYAFIEELEGLREDMIIKSLPGEIKVSLGIHSGFVTRALLGPPGQQKISFFGDT
                     IGIAARLDSLC"
     assembly_gap    14994..15093
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15094..15480
                     /locus_tag="PLS88_05530"
     CDS             <15094..15480
                     /locus_tag="PLS88_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06836.1"
                     /translation="RYQKIPQQGKTPAMRELSVNLSRENAPAMLKLFPGIDKRIIDSL
                     SPPALEADPVSAEEYRLNLEQVIIGKKNMPAFDACAVNIAITAPKVIVSATDGSISGT
                     VFRMRIPLFALLTLENPISFSLRWNE"
     gene            complement(15449..16423)
                     /locus_tag="PLS88_05535"
     CDS             complement(15449..16423)
                     /locus_tag="PLS88_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU06837.1"
                     /translation="MLYTFDFHNHSCLSPCASLENSPLEMVERALRKRIDLFALTDHN
                     SALNTPTFAIECANAGIIPLFGIELNPFEEAHLLAIFSDPLTALRFSDWAFGYLPAIQ
                     VDPLQFGDQVVVDPLGQILSMPATWYGSALKESFTFFAEEAAKAGAIVIPAHIDRPHF
                     SVYSQLGFLPPGHYDAVEAMSPNPEPALCGDYCVISNSDAHVLEHIGRRPSLIELDEK
                     KVADMRVGLTRIAEAWHKAVSTNQTNQGASSRKELEEDVEDQTCEAGLSIKISGLLPL
                     ISFLREWYPFFEAQSLFDNIRHSLHSRRAWSVYKMPESLIHSTAKKKK"
     gene            complement(16442..16789)
                     /locus_tag="PLS88_05540"
     CDS             complement(16442..16789)
                     /locus_tag="PLS88_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06838.1"
                     /translation="MTTKDVPGLISAEVLQGDFEDRAVKIVYTSDLLSDVLANGRDAS
                     VLVTIQAHKNTVAVASVVDIPLIVLCNNRTIIDDMIETAAQSRIGLVRTALSQFEVSG
                     RLWQALHAPESPR"
     gene            complement(16991..17431)
                     /locus_tag="PLS88_05545"
     CDS             complement(16991..17431)
                     /locus_tag="PLS88_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HOU06839.1"
                     /translation="MNSFSIQTQFWIMAISILVFIIAIVAVILAYKAGKKHGFLDAQL
                     SENERMEHARKDAVGRSRAVISGQISEQIAPWLPNFPVNPSDARFIGKPVDFVSFCGA
                     DEGFVQEIVFIEVKSGRSALSTVERSVRDTIVEGRVRWVEYRIE"
BASE COUNT         4650 a         4338 c         3697 g         4733 t
ORIGIN      
        1 atggaaattc tttgaaacct ccgaggttct ctattgtctc gaaaatggcg tcgaaacttg
       61 cttgtcgcat cgagcggacg gcggggtatt ttatgggatg gctggcaggc ttattttgtg
      121 acttagatag cggcagccca agttgcgctt gatgtggcga tggctgtggt gcctctcttg
      181 tcggcgcttt cggcgctgct gtcatggccg actgcactgg ctcttctgga ggttctgcca
      241 gccaaagcgg cgctggctgc gctgccgttt gcatccgctg cgctctaaga gcccttgggc
      301 tctctgcgct tagccgacga taatatcgat gcacaacttt ttcggcgagc cttcgattca
      361 agccatatac aaggattgca tccgcatcta tgccagcagc aatttcaccc atttgaaggg
      421 ctcgccaagc acgatcttct cgatgattga aaacaagcac acggaaagtc tgctctgcgc
      481 tgttgaactc ttttatttcc gagagaattt catgtgtctc attctcctga gggccatagt
      541 tgctatctaa ttcattcaag ttgcgcatat cgctcgggta gcacaagtcg ctcgaataac
      601 gcaagccacg ctcctcatgc cacatctcaa gcgtagactg agggtcgttt gccgcaaagg
      661 cattgatgac tcgaaccttg tgtattcgcc agcttgtgag aatcgtggat ctcacgccgg
      721 gatctggctt agcgccttgc atgccgcgca gcgcaatttc acgcgaaacg ccgcaagcgc
      781 tgcagatcgc aagcgcaaga gccaggttct gtgcatgtat tgggtatgca aatgcgctgc
      841 gcagcgcttg cgtttcttcg tcgatgggca cgaaggcaag atgggcaccg cgctgcgcgg
      901 ccatacgcga aaaaacagcc tttatttctt cgtccatgcc ctcgctgcct acaaaggcct
      961 tccctctttc cggaatagac tccccaagag cctcggccac cgattcaagc tccgtcccca
     1021 ttgtatccaa atgatcgagg cgcacattag ttatgactcc tattgtcgaa tgagcaagcc
     1081 gcgtctcgag cacaccaata gtctctgggc gaattgccat acattcggcc actatagcct
     1141 gcgccccagc cttatatgcc gcccgaacga accatttgtg ttccattatg gaaggccgtc
     1201 tctttgccga accatgtttt cttatctctc tctcgcttag atctggcaaa ataatgcggg
     1261 ctgcagaacc ggtggtcttt gcatatactc ggagaccccc agctgcaagc cctgccgcaa
     1321 tgagccttgt cacagtagac ttgccgcgtg ttccattgac aagaatgcgt atgggcacgg
     1381 ctttgaggcg cctacaatgg gcctggtatt cgactattgc agccattgcc aggcttatgc
     1441 tgactatgat tacaagagag atcacggctt tatatctttt ctcccataga ttctattgtc
     1501 tcatgttata aggcactcgt tctatagcat taagcctcag gctagctctc tagcttgctc
     1561 gagtctagat atagagatga ctatctcgcc agccgcaggc ggactcagta tcagaatgct
     1621 accataggat atctcgatac cagagagaca tgcaccatta tgatgatctg ccgaattagt
     1681 cattacattg taccgaattt ctactggcgc cgatccaaat tctcgaggtt tccccaaaag
     1741 aatttgaaga gaatcttgtc ttctttcttt atcgcttcta tcatcgctgc tttcattagt
     1801 tatgagccag actatagcct tcttctggga ggcaacataa tagcagccta tcttttgcac
     1861 actgctgggt actttcctca ataatctaga tgcgtcttcg cttgtttcga agcccttcgt
     1921 catgtcaaga tcaatacttt gatcagtatt gtgtttccat cgcaagagaa atgacgaaac
     1981 ttgctgtaga gctaggtctt tggagagata aaccgtatcc atttatgtac ctattgagca
     2041 aatcaagcaa aaagtacact gcttagattt tattccatgc cgagcagcca tttttgtact
     2101 tcttcttttg aagaaggcaa gccttcgata ttcaaagcaa ctaaatctcc cagctcttcg
     2161 taagcagaag ccacaagagc ctttagtgtg cagagatgta tatagatcct ttctgcgagg
     2221 ggagctttat cattgtagat ttgatccacg gtcggatcat cgcttggttg tgcaggattc
     2281 agtgtttcca caaggaaagc tcgaatacct agctttcccc actcccaatg ggaatagcct
     2341 gcaaactcct ctcttgagct ctcaaggtga aatgattttc ctgtctcttc ttctatctgg
     2401 agcacagcaa gtgcggctat atcgagatat tccggtcttg taataatgga ccatgcaaga
     2461 gaagaaccag ggcttgcttc gtgcagatcc agtgccgcgt tcacatggtt ctgcatgaga
     2521 agctgggtaa ttgcatacgc agtttgactt gtgcgattac ccatcgggtc gcctgggtac
     2581 cgacggttta gattccgtac ttcctggcca gataaagcag gaaaagattc caaagcagag
     2641 gctggtataa aaaaatcagg gtctgggcca ttttcatggg caaggttgga aagtctcgct
     2701 ccatagtgaa gaatgggtgc agcagcatta cttatcgacc atgttgcggc cgaagcattt
     2761 gctcgcggta caacaatgag cgtgccgcca gacactctgc agtgctcgat tatccaatat
     2821 gccgccagag ttcctgcaat ttcatttgca tgggcacctg ctattatggc aacggtataa
     2881 tctttcgaag tgctctcctg cctttcggca acttttcccg aaagaaatcc ttcgggtgcg
     2941 tttttcgtaa gcaatcctgc gatatctccg agaaagaata ctggcgtgtc aaaaggagtc
     3001 cccttaactt gcgacgaaaa atcagacagg gaaagaatct tttcgacacc tttccctgcc
     3061 tttacttctg acataactaa tgctacgcca ctttctgttt gagcttgcga aacaaagagg
     3121 ccaatcaaaa caaaccaaac aagaaagagc tttttcattg cgctgcaaaa aggctcataa
     3181 gcacattacc tgcttcagcg atcgaaggaa cttgaacaag ggggacgttg ttcttttcgc
     3241 ataaagaagt aaaaaagccg tcattgttac cgccctcgac aacaataaca acattggaaa
     3301 cagggacaca aagctctatt acttgatttg aggcttcacc tcgacgggcg gttcctcctg
     3361 tatgtataga aatgattcgc gtattattct tgagagcttc tgcaacgatg ttctttacgc
     3421 gctcgatttc ctgctcaagg ctcaatccag cagcgccaag acctttgttc gagacaccaa
     3481 ggacgagcac taatgtcttt gcattttcta ttgcctgagg ctttgccgca atatcataat
     3541 caagaccgag tttgagcctt gtgttggcta gcactttgac aattgcagca tccgatgtct
     3601 gacctgctga agttatgaat cctggacgct caaaattcgc tacaagattc gatgggacag
     3661 aatattgcgc aaaaattggc aatatactta aggccataaa gcatactatg gctagaaatc
     3721 tctttgtact agggtatcta tagatcatat gccacacctt caaaatccga agaccgcacc
     3781 atcacgccta gggtctgccc ctccatgcat gattcccgtt ttcggatcta ctagaatcgc
     3841 ctgtgctcca ccaaaaaaga ggtcaaaggg tggatattcc tttatgctgt atccaatcga
     3901 tttgagccat tcctgagttt cttttggaat tctcgattcg atagacagat ctttttctgt
     3961 atcccgcgca tagaatctag gagattctat tgcttcctga acacccatat caaaatctat
     4021 gagattaacg gtcaaaatga ccatagtgga aattattctc cctgcgccag gtgtccccat
     4081 ggtcacaaat ggaattccgt cctttgcgat gattgtaggt gctatagtag tgcgcattcg
     4141 cttccctggc gcgtaggcat taggaccgcg cgaagaccaa ttctgcattt cgttgttgag
     4201 gattatccct gttcctggca ctgcaacgca ggctccccaa aatcccgata gggtttgtgt
     4261 gagcgctacc atgttcccgt gacgatcgac aacagaaaga tgagttgtag atgggtgttc
     4321 attgggtgtt cctgccttta ttgatgtcgt aatcttattc aaatcgatct cttgggcacg
     4381 actccttgca tattctttat caagaagacc tttaacaggt atctgagtaa aatcaggatc
     4441 gcctacatag gctgagttat ctgcaaatcc tcgtttcatg acttcagaca tgatgtgaac
     4501 atttctggga gaaagcggct gttccgatcc taaatcaaaa cattcgagca tgttgagcat
     4561 ttctatcaca gaaattccac ctacaggcgg aggcgctgat acgatttcat aaccgcgata
     4621 tgtaccacgc acgggctcac gcttgatcgc tcgatatgca gccagatcgg cttttgtaat
     4681 aaaaccgcca ttttttgcca tatcttttgc aatcgcttcg gctatctctc ctttatagaa
     4741 aacatctggc cctcctgcag cgattttttc aagagtgacc gctagatctg gattcttgaa
     4801 tatatcgcct gtctgcaaag gcaaaccttc aggcgcaagg atctgcaaaa gtgactcatt
     4861 tctcgaaata ggatcaaagc gatctgcaat tgtctgcgca agtgtctcgc tgacgataaa
     4921 cccattgcga gcatagcgaa tagctggtgc cataacctca gccaagcttt ttgttccata
     4981 gtccttgagc gcagtacaaa gacccgctac tgtacctggg acagctacag ccacatgacc
     5041 ttcactacca aattttattt tcgacatatc cgcaagaggc gccatcgaac ggtaatcgat
     5101 cgcaatagtt gtcttgttct ctgcgaggta tatgaccatc atgccttcgc caccaagacc
     5161 agttgcattt ggctctacaa cccctagggc aaaggctgat gcaatggcag catctaccgc
     5221 attgccacca gccataagaa tttcgagacc ggcagcggaa gcaagtggct gagcagaagc
     5281 caccattcca ttacttgcgg taatctcagc cttaattgta tcagcagcag ataaagggag
     5341 agcaaaaaca aaagccgcta ataaaaatag caagaaattt ttagcgccat gcttcattgc
     5401 taagccaaca ttcttgttct tttgaaccat atcaaaaacc tcctcaatga taattagttt
     5461 accagtttag aattttgcca ataccctgtt ccttgatgtc ctgccagtta ggaaacatgg
     5521 aaaattcgat atgcttgtct ggaagggaat cggaattcca tgcatccaga attgcttcaa
     5581 ttgtagctaa atgtgttgct acacgctcct cgagaggaaa cttcgaatct tctacaggat
     5641 tccctttatc ggttgcttgg gctggattcg gagtctcaat aagaaaagca aacgcattcg
     5701 tctcgtctcc ccattctcta tgagaaagac catgaaatac gtcgctcgaa ggttccagtt
     5761 tcatattgat tccttccaat tccaggttga ttacagccaa ggcaccaaga tcgaggttct
     5821 ttggatttgc gacaatcatg ttggcaagac gtgattccgg tccagcttca tgcaaatcga
     5881 aagcgatgtc cactttctca tttttgagca aattcatcac tgcccatgca agctgttcgg
     5941 ttaaagtgcc gtcaggtttg ccaggataga cacggttgag attgcgagac tcggagccct
     6001 cgaaagaatc acctcctttt ggatgcttat agcgatcggg atcggtcatt ccttgatgca
     6061 ctggatcggt atatcgcgca ccatatttga aaaatcgagc accatgaggg ctgtcgattc
     6121 tgatccacga aggtacaata gtgctctcag tccatgtcga agcgctcata ttgatgtctg
     6181 gaattactat aagtctacca taggaaggaa ttgcgttctc gacaagtatt actgccgaca
     6241 ttataccagc aatttcgttg gcatgtgttc caccggctac aaagactgtt gctccaggtt
     6301 tttttgagtc ggcaatgaac actcgagtat cagaaggtgt tccttttagt ggcgcaaacc
     6361 attctgaaag ccataaaatt tgcgtaatcc ctttgcctgg gcgaatatca tgtaaaatag
     6421 tatttgcacc aagagatgga tcagaacaaa tcgggctcaa aataaggcaa gagaaaatac
     6481 atataagcaa taaaaaaaca gatcggcgca tttctatcga ctccagcaaa aatacttatt
     6541 aggcacaaat catgcctata tagaatgagc cgcgcatttc ctggcacact cggatacgct
     6601 ctggcattct cgagcgcgca aggcgcggct cttatcaatt agttatctag ttgtgcattt
     6661 tgatcatgta atacttatcg ggcactttga cctgagtggt aggcaagaag cctttgccaa
     6721 aaatataggt atccatgtag atgagaactt gattccttgc tcgtactcca gttgccatgt
     6781 ccgtataata tgctccattc cactttgctc ctggcgtgta cttcccaaat gcatcaaata
     6841 catcttttaa actgtctttc ttcgcattac cttctattac cctcttcgca aattcaccta
     6901 gacctgctgt aaccgtgtac ccatacgaga aagcccaggt accaaaccta cctgctcctc
     6961 ccttgtctat cactgctttc tcaaccttct tcaaaatagc tggaaaatcc cctgcttcct
     7021 ttgacaaatc caatccaaga gcaccaggat atcccatcaa tggcgacggc aaatccgctt
     7081 ccacaaaata accaccatac gccaatagct gcttcaatag tggctccgta tgtgcatcat
     7141 tcgtgcagaa aaatgccgtg tccttcccat acttctgtac ccactgcggt accttctcaa
     7201 ggataaattg ctgcgctcct gctaccccta cgtccgatgt aggatctggc gcagtctcaa
     7261 aataaaagtt aatccctaaa tctttgcacg cctcctccat tatcgctcgc ctgcgtccta
     7321 aggtctcata gctcatgtgc ctaggaaacg atatgtgcac aaagttcttc gcacctagct
     7381 gcttcgccgc ccagataata gtgtaccctc gagacacaaa gtcgttcgat accgcaagat
     7441 ccgccgcttg ttgaattacc agtggatcct catgcggttc ccctgccagt agaagaatgt
     7501 ccggcctctt ggctcgcact cgcttgaagg cttcagccgc gccaggaact gcttggttga
     7561 ttacaatagc cttcatgaga gggtcgtcag caagcgccac cacgttagaa atatatgtct
     7621 cctgctggga cataaaatca tctgggtagg tgatatgctg aatcattcct ccgtctttga
     7681 cgctgccata ctccttgatg agctgttccg ctcctcgcaa gtcgtcctcg gcctgagaga
     7741 ctgtctcggt agctatgccg atgtggaatt tggccttggc ctgagcgaat acatcggcgc
     7801 ccaccactag aaaagctagc gctagaataa ctgctacaag ctttttcata ggacctcctt
     7861 atttgatatt taagatgcag aattactcta cacctagaaa acaccaatca agccttcttg
     7921 cattttttgt gaatattcac agctctatga actatgaact attgagtgcc cttgcatgta
     7981 gtcttttttt atggagttgg cgatgctttt cctaaatcct ccagctgtgg gctacccatg
     8041 ctttgaaagc taatttcctc atcaggcgat atcgattctt gggattcctc aagttgttcg
     8101 gtcacggtaa atggctcgag ccatggttca attccattca ctagcttagg tacatagatt
     8161 attaccatat ctgtccatag aggcacggca aaagactttt cgattttgcg cccatcatcg
     8221 aatatttcga ccttgataat gtgtttttgc ccctgaacca ccgctttatc gcgatctccg
     8281 cgatagtatt caagaggctc ttggttatcg atcgatactt ccattccatc tatggcgctg
     8341 taggagccat catggggatc tttattatct ataagtatgg tatgtcgccg gccagaatga
     8401 atcattaaaa caatgagaag gacataaacc gcaagaagcg ccgcacgaat tatgaggcga
     8461 cgctttttta cattttgggc attctggccg tttttttcgc tcgtggatcc gttcatgcct
     8521 gccccccatt tttcgtcgtg atctcgacct tgttattgtg actattgttt actccgcgta
     8581 gaccaagtcg agcttctgcc gcggccttct tagatctcca tgcgtagagc acgagcgaaa
     8641 gcgctattac accatagccg atgaactggc ggaaatattc tccaatcatg gccgaaccaa
     8701 aaagattctg cccagcaagt ggcgatacga caaacattgc atgcaagagc ataacaccta
     8761 tgaaaacatt agcgatattc gcgctcgtta ccgaagcgcc accgaccaga atagaagcaa
     8821 cggcaaagaa ccctgtctga tcgtgtgcat tataggtagc aagattcccc atattttgaa
     8881 ggaagataat ctgtccggca caagcaagca ctgtcgagat gataatcgct attatgcgcg
     8941 tcttttctac tggaatgccg gcggattcag ccacagccat gtcctgaccc acagcccgca
     9001 tgtcctgtcc tagcttcgtc ttcttaaacc aaactatgaa aacacaaagc acggctatta
     9061 caatgaaagt caccactgga atagaaaatc ctgcaatgcg caacactagg atattgtcca
     9121 gcgactgacg aacagattca agattgagcg tattacgaat cccatatccg cgcgacagaa
     9181 caatggcctt gttccccatc ggcactaccg aacccagcat gtacatcaca aagaactgat
     9241 aaattccgtt cataaaaaat gctagaatgt agcccgtcac catctcgcgt ccttttgctt
     9301 tattcatgac aaggccgcag atccatccaa gcagcacaga aataggcaat cctacaagaa
     9361 gagcaaatac aagcccctgc cagcctgcta tgctatagtt cactgcaaaa atgagcccta
     9421 tctgccctgc catagcaccc agagtcatac caaaattgag acccattcca gcgtagatag
     9481 gcaaaagcag tgcaagcaca aggaaggaat ttcgccccaa tcttgtgatg agctcctgta
     9541 gtatatacct aacagataac cctgatgggg gagtcgctgc tattgtgatt atcagaaaaa
     9601 tgataactac ggcgtaccgt gataggaatg tcgagaattt tttctttgcg ctcattttga
     9661 caccttcatc ttgcgcgtga gcgcgtagag aatcatgccg ttcgatacga taatccgtat
     9721 cacttccgac atatcggtat gcaaaagcga attgataaca gagggagtca tagtcaagag
     9781 accttggaaa aggattgttc caacaacaac attgaccatg ctcgctttat tgatactggc
     9841 tcctccaata aggatcgcag ctactgctgg gaaaaccatc gcattaggcg ccgtatagag
     9901 ttggataaag ccgaaactct gttcatagac tattatgcct accgcaccga gaaatgtgga
     9961 tactattacc gaaattgtcc tcatcttgtc cacatcgacg ccagatgcgc gagcaaaatc
    10021 tgggttcgac cccactgctg tcatagcagt gccagtcttg gtgcgcaaaa aaacccacat
    10081 aaagaaagca aaaagagcta caaataataa cagccccgta ggaaatatga agaattcccc
    10141 aattttaatg gctaggaaat tattcagtac atgcagccaa tagttttcga ccgaaatcgt
    10201 tgtgcgcaag cccttaccag catagcccca gaccattgtc ggattatcgt aaggcaacac
    10261 tagccacatg atgcacatga acatgatgaa agagaagccc acatacattg ctatagtcat
    10321 ttcctcgcct ttgactctat tgagtagctt gccatatatc cagccaaaaa gaatagaaaa
    10381 tggcatggct aagacgatgg cactgaggaa tccaatcatt ccatgcagct caagctgaag
    10441 tgacaaggtt gctcccaaaa gtccagacac aagcccaagc gccaagccga agttaaggcc
    10501 gcaccccgat tgaatcatgg ggaccattgc caacaccata atagcgttct gtccaaatcg
    10561 gtttagaaca tcggaaatcg atgcgtcgag ccgcacttgg acaaatggtg ccataataaa
    10621 gagtccaacc aaaaaaagaa aaataatgat gcgcggccag ccgaaatctt gaatgaatgt
    10681 cttgatatta cgcattgacg gcttcctcca tagctgcgcc cgacataagc aggccaaatt
    10741 cagagatatc tgccgtcggc ggcaggatcc ctgcaattct tccttcatcg acaattgcaa
    10801 tgcgatcgca gatcgaacgc aattcgataa gctcgctaga gaccataact atggtggtcc
    10861 caaaatcgcg attatagtga tgcagagcgt ccaagaccaa ttttttagcg ccaacgtcta
    10921 ttcctcgtgt tggctccgat acaaataaga gctgaggctt gattgcaaaa gcttttgcta
    10981 agcagatctt ctgctggttt cctccggaaa gttccttggc tttctgcttt gtactggtac
    11041 atttgatgtc cagaagcttt acatattctt ggcaaagctc ttccatggcc ttttcgtcgc
    11101 gctgtgtgaa aagcccagca agcattctct taaggcattg gttgtgtatt tgcaaagcag
    11161 taaaagctat gttccactct aggctttcat cgagcaatag gcctactcca cgcctgtctt
    11221 ccgaaacaaa acccatgccc attgcaagcg ccattgcagg gttgttgagc ggtactggct
    11281 ttccgtcgag taggacttct cctcctgcgg ggaataagcc catgatccca ttggggattc
    11341 cgagttttcc ctgtccagcc aagcctccga ttccaaaaat ctctccctta tggacatcga
    11401 aggaaacatc gcggacagtc tctcctggca tatcaaccca gagatttcga acatttaggg
    11461 ctatgtcgac aaagcttcta agctggtatc tatcttcctc tcctgcgatg gtgcgaccta
    11521 ccatccagga agcaacatct cgcaaattga tgttctgatt tggagtgtcc tttacaagat
    11581 gaccatctcg aagcaccaca atacgatcag caatagagag aacttcctgc agtctgtggg
    11641 aaataaagat aatgccgata ccttccgaag caaggcgctt gagtgtctgc aatagcactt
    11701 ctgcctccga ttcggtcagc acggccgtag gctcgtccag aatgagaatg cgagtctgtt
    11761 cacgatcgat ctctctcgct atctctgtaa actgcttgtg acctactggc atttcggaca
    11821 ccagcatatc tggatcgaga ttgacaccaa gcttgtttat ggctttttca gcacgctcac
    11881 gcatcttatc tcgcagaagc acactcatac ggtcgccaaa tacatcgttt agaacagtag
    11941 ggcgcattgg ctcacggttc agcacaatat tctcagtggt cgtaaaacca ggaataaggc
    12001 tgaattcctg atgaaccatg cccagcccag catccaaggc gtcgaatgga ctctcaaaat
    12061 gaacctccgt cccctcgatc tttatgctgc cttggaaacc gccagtctcg gctatgacag
    12121 gcatactaaa aagaatgtgc atgagcgtgg ttttgccagc gccattttct cccacaaggc
    12181 caagaatttc tccctcatgc agatcaaaac tcacatcgtg cagtacgata tttccataga
    12241 aatctttaga aacatgctcg attgaaagaa gagaacttcc atttatcata ataattccat
    12301 tagaaatggg tttataaaaa ttggcccgca tcccgacaac gcctgaatac gggccgaaat
    12361 aaacgcttgt tcggcgtctc aagctaaaaa catagaacca agcgcggaaa aaatttggcg
    12421 catggctctt attaactatt tgtgcatctg aatgttgtaa tacttatcgg gcactttgac
    12481 ctgagtggta ggcaagaagc ctttgccaaa aatataggta tccatgtaga tgagaacttg
    12541 attccttgct cgtactccag ttgccatgtc cgtataatat gctccattcc actttgctcc
    12601 tggcgtgtac ttcccaaatg catcaaatac atcttttaaa ctgtctttct tcgcattacc
    12661 ttctattacc ctcttcgcaa attcacctag acctgctgta accgtgtacc catacgagaa
    12721 agcccaggta ccaaacctac ctgctcctcc cttgtctatc actgctttct caaccttctt
    12781 caaaatagct ggaaaatccc ctgcttcctt tgacaaatcc aatccaagag caccaggata
    12841 tcccatcaat ggcgacggca aatccgcttc cacaaaataa ccaccatacg ccaatagctg
    12901 cttcaatagt ggctccgtat gtgcatcatt cgtgcagaaa aatgccgtgt ccttcccata
    12961 cttctgtacc cactgcggta ccttctcaag gataaattgc tgcgctcctg ctacccctac
    13021 gtccgatgta ggatctggcg cagtctcaaa ataaaagtta atccctaaat ctttgcacgc
    13081 ctcctccatt atcgctcgcc tgcgtcctaa ggtctcatag ctcatgtgcc taggaaacga
    13141 tatgtgcaca aagttcttcg cacctagctg cttcgccgcc cagataatag tgtaccctcg
    13201 agacacaaag tcgttcgata ccgcaagatc cgccgcttgt tgaattacca gtggatcctc
    13261 atgcggttcc cctgccagta gaagaatgtc cggcctcttg gctcgcactc gcttgaaggc
    13321 ttcagccgcg ccaggaactg cttggttgat tacaatagcc ttcatgagag ggtcgtcagc
    13381 aagcgccacc acgttagaaa tatatgtctc ctgctgggac ataaaatcat ctgggtaggt
    13441 gatatgctga atcattcctc cgtctttgac gctgccatac tccttgatga gctgttccgc
    13501 tcctcgcaag tcgtcctcgg cctgagagac tgtctcggta gctatgccga tgtggaattt
    13561 ggccttggcc tgagcgaata catcggcgcc caccactaga aaagctagcg ctagaataac
    13621 tgctacaagc tttttcatag gacctcctta tttgatatta aagatgtgaa attattgtac
    13681 accttaaaag gaacgcgtca agtatttctt tttttctcca agccagtttg ctcagacaat
    13741 cttgggtctt tccagtaaat gctaggacat atacaataga ttcgatgcag ttctcatgga
    13801 aacgctttgc agacgaactt tattattact cgaaccagtt tattctcttt tttgtgctga
    13861 taatatcgct tacctcggga tcatggaaaa acgcactcgt cacttcgctc attgtgcttt
    13921 cttttatgct aattcagacc ctactgctga ctattcaagg acatatccca atactgaggt
    13981 tcatttattc ttttattacg cctgctggat actcgattct tcgtgcaacg ctgtcggata
    14041 tagcattcac agagacagtg aacttgctgt tgtggggagc ttcggtttat atagcgattt
    14101 ttcagacgct tacacttatc ttgcgtaagc caattctcaa acggttttca gaggccgcac
    14161 tatcattggg atctgccatt atctttctac tcttttatgc atatcttgat cttcgcatat
    14221 cggtcacagc ctcttttgta aaaggagaaa tagatgctga ggcaatggct caagcattag
    14281 gtataagatc ttttttcgcc gtcttcaaca aattctttca agccccccaa cacctgtttg
    14341 cgctattcgg tgtaatatcc ttcgatttca tgctaatggt tgcaagatgg agagcaatca
    14401 acctggaaca gcgactcacc agaataatcg aaatgccaca ggaagctaag cgaatctact
    14461 catcatctcc ggaatccgaa aaaggcgcat ctcctctaga atatgaaggc caggaagttc
    14521 agccggtatt aactaccgaa acccaagata gtcttcctac aaaaactata gcttctctat
    14581 tcgtgacagc catctcctcc gatattattg gctttacaga tctttctgag cagcttggac
    14641 acaaggaggc gactgcctta ttaaatagat attatgcaat ctggacccgt tgtgctggta
    14701 gctatggagg aagaatcgta tctatttcgg cagatacggt ggttatattg tttggccttg
    14761 tagatcaaga gcgcagcgcg gaccgtgctc tcaatgcttc atacgctttt atagaagaac
    14821 tcgagggact cagagaagac atgatcataa aatcgctccc tggagagata aaagtttcct
    14881 tgggtattca ttcgggtttt gtaaccagag cacttcttgg accaccgggc caacagaaaa
    14941 ttagcttctt tggcgacaca atcgggatag ccgcacggct ggactcgctt tgcnnnnnnn
    15001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgctatc aaaaaatccc tcagcaagga
    15121 aaaactccag ctatgcgcga gctttccgtt aatctttcga gagaaaatgc acctgctatg
    15181 ctaaagctct ttcctggaat agacaaacgc ataatcgata gcctgagccc tccggcgctg
    15241 gaggctgatc cagtttcggc agaagaatat aggctcaacc tcgaacaggt cataataggt
    15301 aagaaaaaca tgcccgcttt cgatgcatgc gctgtaaata ttgctataac agcccctaag
    15361 gtgattgtat cggctacgga tggaagcata tctggaaccg ttttccgcat gagaatccct
    15421 ctgtttgcgc ttcttactct agagaaccct atttcttttt ctttgcggtg gaatgaataa
    15481 gagattctgg catcttgtat acgctccatg ctcttcggct gtgtagagaa tgtctgatat
    15541 tgtcaaaaag cgattgtgct tcaaaaaatg gataccattc ccttaaaaag gatataagtg
    15601 gcaaaagccc tgaaatttta atcgaaaggc cggcttcgca tgtttggtct tcgacatctt
    15661 cttctaattc ttttcttgat gaagcgcctt gattcgtctg atttgtcgat actgctttgt
    15721 gccaggcttc agcaattctc gtaagcccca cacgcatatc agcaactttt ttctcatcga
    15781 gttcaattag ggaaggcctg cgccctatgt gctcgagtac atgcgcatcg gaattagaga
    15841 tgacacagta atctccacag agagccggtt ctggatttgg gctcattgct tccactgcat
    15901 catagtggcc tggtggaaga aatccaagtt gactgtaaac cgaaaaatgc ggtcggtcta
    15961 tatgcgctgg aattacaatt gcgcctgcct ttgctgcttc ttctgcaaaa aaagtaaagg
    16021 attctttcaa agcgctgcca taccaggttg cgggcattga aaggatttgc cccagagggt
    16081 ctacgacaac ctggtcgcca aattgcagag gatcgacttg tatagccggc aaatagccaa
    16141 aagcccagtc actaaagcgg agcgctgtca atggatctga aaaaatggca agcaagtgcg
    16201 cttcttcaaa aggattcaat tcgatgccaa atagcgggat aatgccagca ttagcacatt
    16261 cgatcgcaaa tgtaggagta ttcaatgcac tgttatgatc cgtaagcgca aagagatcga
    16321 ttctcttcct aagagccctc tcgaccatct ctaaaggaga attctcaaga cttgcgcatg
    16381 gacttaggca ggaatggttg tgaaaatcaa aagtatagag catggacaat gtatgtccat
    16441 tttaccttgg cgactctgga gcgtgcaaag cctgccaaag ccgaccagaa acttcgaact
    16501 ggctaagagc cgtgcgcacc aatcctattc gagattgagc ggcagtctcg atcatatcat
    16561 ctatgattgt ccgattattg catagaacaa tcaaaggaat atcgactacg ctcgccacgg
    16621 caactgtatt cttatgtgcc tgaatcgtga cgagcacact tgcatctcgc ccatttgcca
    16681 gtacatctga aagtaaatcg gaagtataga ctatcttgac tgctctgtct tcaaaatctc
    16741 cttgaagaac ttcggcagaa ataagcccag gaacatcttt agtcgtcatg cgatggtact
    16801 ccttcgttct tgcttttgtt tcggttttgc catcatgtca taaaaattaa cagtagatcg
    16861 attttcggta tggcaaagcg gatggcaata gagttactta tattatagcc gtactttgca
    16921 tttcaataaa gctggaagaa tatttcacaa aacaaaacaa tttgcatagc ttttcccgtt
    16981 taccggtttt ctattctatc ctgtattcca cccagcgcac acgcccctct acaattgtat
    17041 ctcttacact gcgttcaacg gtgctcagcg ccgatcggcc agatttgact tctatgaaga
    17101 cgatttcttg tacaaatcct tcatcggctc cacagaaaga aacgaaatct actggcttgc
    17161 caataaacct tgcatcggaa ggattgactg gaaaattcgg aagccatggt gcgatctgtt
    17221 cggagatttg accggaaatt acggccctcg atcgccctac tgcatccttt cgcgcatgct
    17281 ccattctttc attttcagag agctgcgcat ctaagaatcc atgtttttta ccagctttgt
    17341 atgccaaaat aactgcgaca atggcgatta taaaaactaa aatcgatatt gccattatcc
    17401 aaaattgtgt ctgtatagaa aagctattca tgcgtatagc atagacgatg atctctcaaa
    17461 gtaaaagatt actgctacga accttgtata aaaagttgaa gcataaagta gggaagag
//
LOCUS       DAOSOB010000040        17515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3264_length_17515_cov_76.427892, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000040 DAOSOB010000000
VERSION     DAOSOB010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 17515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17515
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3264_length_17515_cov_76.427892"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..117)
                     /locus_tag="PLS88_05550"
     tRNA            complement(35..117)
                     /locus_tag="PLS88_05550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(82..84),aa:Leu,seq:caa)
     gene            391..1290
                     /gene="speD"
                     /locus_tag="PLS88_05555"
     CDS             391..1290
                     /gene="speD"
                     /locus_tag="PLS88_05555"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOU06840.1"
                     /translation="MVRRVKGRTIKLQGFNNLTKSLSFNIYDVCYARSRDSQISYLEY
                     IDEEYNSKRLETIVEDVTQIINAKLVSISTQDYEPRGASVVALINEENSIQQEPSKPR
                     LFDHAEELLDTAEIKDQEFPLDELEKQVSNAPSIVGHLDKSHIAIHTYPEYHHLTGLA
                     SFRVDIDISTCGMISPLSALNYLIENFDSEVVIIDYRIRGFTRDTKGRKIFLDHDINS
                     IQDYIDQEISNRYIAYDINLISENNFHTKMRKKEIRLKDFLFGDEVKNLSAKEKNRIK
                     RALIHEIQEIFECQNLEMRSGRI"
     gene            1353..2282
                     /gene="speE"
                     /locus_tag="PLS88_05560"
     CDS             1353..2282
                     /gene="speE"
                     /locus_tag="PLS88_05560"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOU06841.1"
                     /translation="MRTIIEHNNPHCGFFYEIGSVIVHRKSKWQDIELAESPEFGKVL
                     LLDGITQVGFKGEERYHETLVIPAMIAHPDARKVLIIGGGDGCVLREVLAFKGVRQVV
                     MVDIDSEVISVSKEYLTELNKKAFEDSRLMLEIGDGRAFVEQCPDNSFDVVIMDMTDP
                     FGPSERLYTKEFFMQIQRILKGPEGVFAMHGESSIARPIGWACIYRTLKAVFPIVRTA
                     FAFVPMYGTLWSFQFSSDATDLLTFSNPIIKPLDELEACIAEKRSEEPRFAKAAMWPS
                     LFAEDHTAKAALSFPQSRVITDSEPHFPDAFEP"
     gene            2370..3314
                     /locus_tag="PLS88_05565"
                     /pseudo
     CDS             2370..3314
                     /locus_tag="PLS88_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            complement(3296..4354)
                     /locus_tag="PLS88_05570"
     CDS             complement(3296..4354)
                     /locus_tag="PLS88_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOU06842.1"
                     /translation="MKSSKIKSSKFFSWHFFVIGFVLTCAIATRPPRLCAKELSKTDF
                     VLGTLCTIRLVEGGNSATLDEAFARLRTIEDRMSVNKEGTEVSKINEMAGKEAVQVSS
                     DTFYVISKAIEYAKITNGAFDPSVGPLVKLWNIGEGGEKVPPSKDILSAKALVDWRNV
                     VLDASTNKVLLKKQGMRLDLGAIAKGYAADEVEKILTRAKVKAAVIDLGGNVFVYGTK
                     KDKTPWRVGIQNPESERGDYLGIVEGPQMTVVTSGVYERYFIENGKRYHHIMDTKTGF
                     PVDNGLLSVSIIGQSSIDADALSTSLFALGIEKGMELLKQFPYTYAIFIDKYKKIYLS
                     PGAEKVFTLLDKGYSLVR"
     gene            complement(4431..5123)
                     /locus_tag="PLS88_05575"
     CDS             complement(4431..5123)
                     /locus_tag="PLS88_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU06843.1"
                     /translation="MASTIWRLKVGPIGENCYIAESEGKIILIDPGADPEKIDNFVRY
                     KQLKPSLIVLTHGHLDHTAAIPDLFELWGTELPIAIHPEDACYLGKESEETNRLLFEA
                     IRAPSYFRASWKPLPAPEILLIDGTMVPGTSLQVLHTPGHSKGSICLYEPELKAGSYG
                     YEEEGREQGTSCSCIISGDTLFRDGVGRTDGPDSDIYALEASLRKLARFSPDTLVYPG
                     HGPRTTIGRELG"
     gene            complement(5130..5771)
                     /locus_tag="PLS88_05580"
     CDS             complement(5130..5771)
                     /locus_tag="PLS88_05580"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HOU06844.1"
                     /translation="MQDNNDAAASRFDEKALKAFLDGLLDELRHPQDPKLLEDVRRAF
                     RRKIPFHLRSYAAALMILRAAGLSRAKPQQKTSPQKTYPQKASREQGKSLFMGTPSAA
                     ESIKGAPSGAISSSPLSSMKELPSKETVSLFVSMGKRHHLKPQDLRNKIAERTGLSLD
                     AIGRVHLLEKYSFVEVPVEEAQKVIAAMNGAELNGHALEIKPAKKRAKTSSKG"
     gene            5931..7457
                     /locus_tag="PLS88_05585"
     CDS             5931..7457
                     /locus_tag="PLS88_05585"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOU06845.1"
                     /translation="MEKNNRPKICLCMTGKTIEDDLRVLDQYRSVVDCVELRADCLDP
                     SERFLIRDFPEKAGLPCILTIRRLKDGGKFEDGEGVRLVLIAKALSFPKPDASANYAF
                     VDLEDDFRAPVVEEACRTFGTRIIRSHHYLDGMPDSLDDAYEELTLEEDEIPRLNVNP
                     QNSEDFIRFLEWCLQKPKTDQLLIASGNYGIASRILSWRLGSLWTYASPLRSGMEAAA
                     PGHIDPLDLINIYNFDLVDSDTTIYTLMGQNSIAQSLSPYLHNNAFRKMGKNALLIPT
                     PVDSCIDGLKILEILGGKGAAITVPFKQDILPYLSFHSTDVQLIGACNTLVWRDSGWA
                     GYNTDADGFERSLLEFLGKQDISGCKATIIGAGGAAKSVALSLFRMGARGLVLNRTYS
                     IARDLARKYNFLYGHLDDRAIDLISEHSDLIVQATSVGMENEGDPISFYEFKGSEAVF
                     DLIYYPVKTLFLKRAEEAGCRTMNGFKMLCYQAAGQYKLWMGEPPPAIYSQLEQPEQL
                     "
     gene            7529..8404
                     /gene="era"
                     /locus_tag="PLS88_05590"
     CDS             7529..8404
                     /gene="era"
                     /locus_tag="PLS88_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOU06846.1"
                     /translation="MPKSAFVAIVGRPSVGKSTLLNALCGAKVSIVSSVPQTTRNAIR
                     GIITKPQGQLIFVDTPGYHLSDKKLNLRLREIVLQSISEVDLILYLIDATREPGKEEE
                     AIAELLSREIHRTVVAINKIDDPEARPLLIEEFVIPRLRGAKRVSISAVEKTNLESLI
                     EILFSLAPEGPFWYPEDIYTDQEPVFRIGELIREKAILNTYQELPHAIAVEYRESSKR
                     ADGVLFARFDILVERDSQKGIVIGSQGSVIKRIREEAEADLKELFDYPIKLQMQVRVD
                     PDWRKSDSRLSRIIF"
     gene            complement(8462..9034)
                     /gene="pth"
                     /locus_tag="PLS88_05595"
     CDS             complement(8462..9034)
                     /gene="pth"
                     /locus_tag="PLS88_05595"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOU06847.1"
                     /translation="MIKLCVFLGNQGQEYRFHRHNVAWLFLDSMIIASELCWSAKFKG
                     QFSQMKLGSTIIYFLKPHTFMNLSGESVGEAVHFYGLGIDELLIVHDELEMPFGAFGY
                     KFSGGLGGHNGLRSLEKHLGTRDFWRLRLGIGRPEHDDVAGYVLSRFDPEQLEVLNKK
                     VFPLAEKTFYEIFSEGIEGFDIRYKKVLPL"
     gene            complement(9031..9951)
                     /locus_tag="PLS88_05600"
     CDS             complement(9031..9951)
                     /locus_tag="PLS88_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU06848.1"
                     /translation="MLSNLHLHSRFSDGMSWPEDIALEAARAGLDIVALTDHDTMGGT
                     ERFIRACETRSLKAIPACEIDIYEPQIEYRSELLAYFPEADFNSSILSTKCILQKALE
                     QRRSRLEFFIESAKELYPDKELSFKDLFKDKTGLDFDEELARKISWSKVDLFLYLKAH
                     RCIKPEMNYKEFKKKFFVGGLLKKFKLDKPEVATVVKAVHGDGGFVVIPHLGHFWDDE
                     LPLFQKEAEKLRLLLAWFRHAGVDGVELYWYNGEFKSSRFNEQLREIAKPQGFFFTYG
                     SDCHGPGSGKHTIMKFKGPFDGFPQRKSIS"
     gene            10020..10937
                     /locus_tag="PLS88_05605"
     CDS             10020..10937
                     /locus_tag="PLS88_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06849.1"
                     /translation="MFQLTDAILERIIFAMEDQSKKLLIDLETGDVIDKTTIENEDSP
                     ELYSEMAQRFVEPPSWDSRRGFSILEAFASTVTSPTDIKMELNAALRRGKGVFKQFRQ
                     VLSKDQDIFKRYQEFKLAAMRPIVERWLEALQEGEKLTALKEEPEDLRDIIASDIDFI
                     VSSLDGIVFKLESYINYCIEESFGSLPIAIKMRSLSDLLELVKTDSNSVHIAMGTIDG
                     VSPLIAGLFSLQAYKGSPICVVHGVFGTKESLMLDLEWPIIECISEYCTKIGATQILL
                     EGPFFSSLLCDSALEYGFSQSGTIFHKNL"
     gene            11071..11991
                     /locus_tag="PLS88_05610"
     CDS             11071..11991
                     /locus_tag="PLS88_05610"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOU06850.1"
                     /translation="MKNCFLFPGQGAQYPGMAKDFYEASKAVRDLFKEASEAASMDLK
                     RLIFEGDEETLRQTRNTQISMVVAEAASALMAREQGITPAGAAGFSVGEWPALAEAKV
                     ITNYDMFRLVSERGRLMEEAGNRAGGTAMSAVLYLSPEKIEQVIHEAGLKQCWIANYN
                     SPVQCVISGTETDIVIAEEKLRQAGAKRVIRLKVSGAFHSPLMQDAYDPFKELVEATQ
                     FSDPKINLYSNVTGKKMLSGEEAKKNASLQIISPVRWIDEERNIAADCYDYCFETGPG
                     TVLAGLWKASGSTIPCIAAGTLQALAAFSL"
     gene            12061..12771
                     /gene="fabG"
                     /locus_tag="PLS88_05615"
     CDS             12061..12771
                     /gene="fabG"
                     /locus_tag="PLS88_05615"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOU06851.1"
                     /translation="MEGVLSGQICIVTGASRGIGLAIATRFKQEGAIVYGLSRTKAEA
                     DIEWISCDVTDELSVESAIDQILKKENRIDILVNNAGITRDGLLMRMKTEDWEAVLNS
                     NLRSAFFLSRAVSRIMLKQRSGCILNMSSVVGLHGNGGQANYAASKAGLIGFTKSLAK
                     ELGSRNIRVNAIAPGYVETSMTQVLPDTAKENLKSSIPLGRPGTPGDIAEAALFLCSS
                     YAAYITGVVLNVDGGMGM"
     gene            12774..14012
                     /gene="fabF"
                     /locus_tag="PLS88_05620"
     CDS             12774..14012
                     /gene="fabF"
                     /locus_tag="PLS88_05620"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOU06852.1"
                     /translation="MSKRKVVITGLGLVSPLGNDVPTFWQNVKANRSGVGPITLIDSS
                     DLAVKIAGEVKNFDPSKKIDPKDAKKMDRFAQFAVYAALEALESSNLPKESLDPIRTG
                     VCLGTGQGGTNTIEESVIRLTEKGPNRVPPMTVAKGLANFGAAQIALSLGVHGPNMTV
                     VTACAAATDAIGQAMHLIRDGHADIMFTGGSEASIARMCMASFINIQALSIRNDEPDK
                     ASRPFDRDRDGFVMSEGAGILVLEEYEHAKARGAKIYAEIAGFGSTCDAHHLTAPDPE
                     GLWVSKAIGIALNDAQLKPEDIDYVSAHGTSTPLNDPIETKAIKNAFGDHAYKLKISS
                     LKSMIGHCIGAAGAIETIAAILGMNENYVHSTINLDNPDPECDLDYVPNKGINMPVRA
                     FVKNSMGFGGQNAVLVITKP"
     gene            14051..16282
                     /gene="glgX"
                     /locus_tag="PLS88_05625"
     CDS             14051..16282
                     /gene="glgX"
                     /locus_tag="PLS88_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOU06853.1"
                     /translation="MPGNPGLLGAIPKAEGVYFSIFSRNATGMILCIYEKPHDDVPVA
                     SLHLDPIRNRTGDVWHCFIPGLKSEVLYLWRAEGPFAPQEGHRFNPYKVLIDPYAKAI
                     TDGSLELCSALAYDENSPEKDLSISTTPNDGFMPKCVVVEDEYNWENDTPLNYPLKDC
                     IIYETHVRGLTKSVTAKVKHHGTYRGVVEMIPYFKSLGITSLELLPVQEFDSSERFRR
                     NPITGEKLSNYWGYSPIAFFAPESSYVFDEGVDHDSYCPSYPVKEFKYMVRELHKAGI
                     EVILDVVFNHTAEGNELGPTLSFRGLDNSIYYMLAEDKRYYRNYSGCGNTLNCNHPVL
                     RTFIMDCLHYWVVTMHVDGFRFDLGSILGRDSQGNLLANPPVIESIAEDPILRDTKII
                     AEAWDAGGAYQVGAFPGGRWAEWNDRYRDDVRRFWRGDEGFTPLLATRITGSSDLYLK
                     NGKRPFHSINFVTSHDGFTMNDLVSYSRKHNEANGENNCDGIAENYSSNYGTEGPSNA
                     PFIEETRNRQVKNFFATLLLSIGTPMLLGGDEFRRTQNGNNNAYCHDNELTWYDWSLL
                     KKHKDIYRFVKCLIAFRKSHNAFRREDFFRGVGKRSIGTIGASGQSKINKYKPNDISD
                     IGTLPDIIWYDQNGLPPQWNKMGKVLVARINERISSGDFPCSYLLAFNASPEAVQICL
                     PHDNEQDIWKRVIDTSFPPPHEIEEKDQPVLSDQQKYLMSPRSFILLISICELDENQS
                     LPS"
     gene            16307..16981
                     /locus_tag="PLS88_05630"
     CDS             16307..16981
                     /locus_tag="PLS88_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HOU06854.1"
                     /translation="MRTSADNEKANLYLRRGLRELSRHKPAQALELFRKSIELTPPSS
                     EKTLSRALYWLSIALLQLNKRDLAVKSLANAQKIHRQGYIRRFYIRHVNEYGMIKQPT
                     KELDDLYAFLSIQLSFYLLNRPSYRFGSEAEHSMVLAFLLHTWKNIKGTEEFKSLDCS
                     EKLLLFNKLKIDFPAFAPYSVVQRKRERQIIPSNIAYNQLCSCGSGLPFMQCCGRIRG
                     ISELLK"
     gene            17190..>17515
                     /locus_tag="PLS88_05635"
     CDS             17190..>17515
                     /locus_tag="PLS88_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HOU06855.1"
                     /translation="MTQNKKLKETRSSANYDDENVLSMYLREINRIPLLSREEENEYA
                     TRAAAGDEKAKEILAKSNLRFVVNVAKKYQNQGLPLADLIAEGNIGLLSAIDHYDVTK
                     GYHFIS"
BASE COUNT         4920 a         3932 c         3889 g         4774 t
ORIGIN      
        1 cgcatcaata accgaaaggc tctataagaa aaattgccgg cagtgggact tgaacccaca
       61 cgctcttgca agcagcagat tttgagtcta ccgtgtatgc catttcacca tgccggcata
      121 aagaatatgg cgtattttgc cgtaggatgc ctttgagagt caagagcgtt tagtaactca
      181 aatcttgata tggatctatc atgatcaatt acgatagtgc tcaataattt tatgcaaagt
      241 aattaagcca atactgcctc gtgataaaat ctaaccatag aacaatcttt accctatatt
      301 gtaaataagg tataataatt agatatgcga aggacacaaa tgccaacgca gccgccttag
      361 ggttgaacac atagagggag gactgtttag ttggtccgaa gagtcaaagg gaggactatc
      421 aagctccagg gtttcaataa cctaactaaa tcgcttagtt tcaatattta cgatgtctgt
      481 tacgcaagat cccgcgattc tcagattagc tatctcgagt atatagatga agaatacaat
      541 tcaaagcgtc tagaaactat cgtcgaagat gtaacccaga ttatcaatgc gaaacttgta
      601 agcatttcta cgcaggacta tgaaccacgc ggcgcaagcg tagtcgcgct gataaacgaa
      661 gaaaattcta ttcagcagga gccatcgaag cctcggcttt tcgatcatgc agaggaattg
      721 ctggatacgg cagaaatcaa agatcaagaa tttcctctcg atgagcttga aaagcaagtg
      781 tccaatgctc ccagcatcgt tggccatctt gataaaagcc atatagccat ccacacctac
      841 cccgaatatc atcatctgac cggtcttgca tccttccgcg tggatatcga tatatcaacc
      901 tgtggcatga tatctcctct ctcagcattg aattatctta tagaaaactt cgattcggaa
      961 gttgttatca tagactatcg aattcgcggc tttacaagag atacaaaagg acgaaaaatt
     1021 ttcctcgatc atgacatcaa ctcgattcaa gattatatcg atcaagaaat ttctaaccgc
     1081 tacatcgctt acgatataaa tttgatttct gagaataact tccacactaa aatgcgcaaa
     1141 aaagagatcc gcctcaaaga ttttcttttt ggcgatgaag tcaagaacct atcggccaaa
     1201 gaaaaaaacc gtattaagcg cgcccttatt catgagatac aggaaatatt cgagtgccaa
     1261 aatctcgaga tgcgcagcgg cagaatctga aaagtctaga aaagtctaca tttgttggaa
     1321 aaagatatta ctctcaataa ggaggaaatt acatgcgcac cataatagag cataataatc
     1381 cgcattgtgg ttttttttat gaaataggtt ccgtaatcgt tcatcgcaaa agtaaatggc
     1441 aagatatcga gcttgctgaa agtcctgaat tcggaaaggt attgctttta gatggaataa
     1501 cccaagtcgg cttcaagggc gaagagcgtt atcatgagac tctagtcatt ccagccatga
     1561 tcgctcatcc agatgcgaga aaagtcctca ttataggtgg cggcgacggt tgcgttctta
     1621 gagaagtgct ggcattcaaa ggcgtcagac aggttgtaat ggtcgatata gattccgagg
     1681 ttatatctgt tagcaaggag tatctgacgg aattgaataa gaaagctttt gaagattccc
     1741 gactcatgct cgaaatcggc gatggcaggg cttttgtaga gcaatgtcct gataattctt
     1801 ttgacgttgt gattatggac atgaccgatc ctttcggacc ttccgaacgg ctctatacca
     1861 aggaattctt tatgcaaatt cagcgaatct tgaagggtcc ggaaggcgta tttgccatgc
     1921 acggcgaatc gtctatcgca agacccatag gttgggcatg tatatataga actcttaagg
     1981 ctgtcttccc catagttcga actgcctttg cttttgtacc tatgtatggg acattgtggt
     2041 cctttcagtt ttcctcagat gcaacagatc ttcttacctt ctcgaatccg ataataaaac
     2101 ccttggatga gctagaagcc tgcattgcag aaaagcgttc cgaagagcct cgttttgcta
     2161 aggctgccat gtggccttct ctctttgcag aagaccatac agcaaaagca gctctgagtt
     2221 ttccgcaatc acgtgtcata acagatagcg agcctcattt tccagatgct tttgagccct
     2281 gatcctagga ttgttccctt ggctcgccag gatttggcga gcttctttag attttctttt
     2341 aatcgggagt accttatgcg tttttatcct ttattttttt gtttgctgcg attatagtta
     2401 tgattatgac gtttgtagaa tctatgcatg tcgccgaagc gcagattatc ctccagattc
     2461 agacttttct gggtcttggt cttcgaattc ccatgacaat tgtatcatta ttcgcatcag
     2521 aagcattcgt gatcgcaatt attcccgttg tatactggtg cgtaaatcgc aagaaagggg
     2581 ctgagcttgg ccttttagtg ttggcttcca cttttataaa tctctggcta aagcaaatat
     2641 tcggatggcc caggcctttc gatatcattc caagcgtagg gcttgcaagc gaatcgagct
     2701 atggtatgcc ttcgggccat tctcagctga gcgtcgtttt ttccgcttat atcatacagt
     2761 ttttgcccaa aaaatggcgt ccatttatgc tgataggctt tccattgctg atcggatttt
     2821 cgagaatata cctaggtgtt cattttttaa ccgatgtcct cgggggttat ctagcaggaa
     2881 ctatcttttt tctcctttat aagctggggg agcctgcaat tgaaaaaatg ctccaaaagg
     2941 cagatttacg agtacgtgtt atattggccg cagctatttc ttttttgatg aatttaattc
     3001 tacctaaaga gacgatgata tcgggagctt ttcttggtgc tgcgctcggt ttcacatttg
     3061 cggctcatgg tctctctttc gaagaaaacg ataaactcaa aatcaaagtt tcccgatatc
     3121 tcgtaggatt ggctactact ggaattgtct acatcgttct caaaatggca agcactccta
     3181 tcaatagctt attgggcaac aatcaggaaa acctccttcg ctttgtgcgc tatactcttg
     3241 ttggtggctg ggtgagcttt ggagtccctt tcttgttcct aaaagtgggg ctcgctcatc
     3301 gtacaagcga gtaaccttta tccagcagcg tgaacacttt ctcagcgcca gggctaaggt
     3361 atatcttttt atatttatcg ataaatatcg cataggtgta gggaaattgc tttaagagct
     3421 ccattccttt ttcgatgcca agggcgaaaa gcgaggtcga aagcgcatca gcatcgatgc
     3481 tgctctggcc gatgatcgaa acagataata agccattatc cacagggaag cctgtctttg
     3541 tgtccattat gtgatggtag cgcttgccgt tttctatgaa gtagcgctcg tagacgccgg
     3601 atgttaccac ggtcatctga gggccttcca caatgccgag gtagtctcca cgctctgatt
     3661 cagggttttg gattccgacg cgccaagggg ttttgtcttt tttagttcca tatacgaaaa
     3721 cattgccacc aagatctatt acagcggctt tgacctttgc cctcgtgaga atcttttcga
     3781 cttcgtcggc tgcatatcct tttgcaatgg cgcccaggtc taggcgcatt ccctgctttt
     3841 tcaaaaggac cttattggtg gatgcatcga ggacgacatt gcgccaatca accaaagctt
     3901 tagcagacag gatatctttt gaaggaggta ctttttctcc accctcgccg atattccaaa
     3961 gttttaccaa ggggcctacg ctagggtcga atgcgccgtt agtgatcttg gcatactcga
     4021 ttgctttgga aatcacgtaa aaggtgtcgc tagaaacctg gacagcctct ttccctgcca
     4081 tttcgtttat ttttgaaacc tccgtccctt ctttgtttac actcatgcga tcttctattg
     4141 ttcgcaatcg tgcaaaggct tcgtctaagg tagcactgtt ccctccctcg acaagacgaa
     4201 ttgtacagag cgtgccaagc acaaaatccg ttttgctcag ctctttcgca cacagacggg
     4261 gaggccttgt tgcgatagcg catgtaagaa caaagccaat cacaaaaaaa tgccatgaga
     4321 aaaatttgga agattttatc tttgaagatt tcatcttatt tcgtttagga aaattccatt
     4381 tgggacgatt tgtcaggcag taattacggc tgatcctcac aatataatgc tcatccaagt
     4441 tctctgccta ttgttgttct aggaccatgt ccgggatata caagcgtgtc cggcgagaaa
     4501 cgcgcaagct ttcttaggct ggcttctaag gcatatatat ccgaatctgg cccatctgtt
     4561 cgtcctaccc catcgcgaaa caatgtatcg ccagagatga tacaggaaca actcgtacct
     4621 tgctctctgc cctcttcttc gtagccatac gaaccagcct tgagttcagg ctcatataga
     4681 cagatagagc ctttgctgtg acctggcgta tgaagcacct gaaggctcgt accaggcacc
     4741 atagtgccat cgatcaaaag aatctcgggg gccggcagtg gtttccagga tgcacgaaaa
     4801 tatgatggcg ctctaattgc ttcgaaaagc aggcggttcg tttcctcgct ctcttttcca
     4861 agatagcaag cgtcttctgg atgaatggca atgggaagct ccgtccccca tagctcgaaa
     4921 agatcgggga tagctgccgt atgatctaga tggccatgtg tcaaaacgat aagagagggc
     4981 ttaagctgct tatatcgaac aaaattatcg attttctcgg ggtctgcacc aggatctata
     5041 aggattattt tgccctctga ttcagcgatg taacaatttt ctccgattgg ccctactttg
     5101 agacgccaga ttgtcgatgc cacgtcctat tatccttttg aagatgtttt tgcgcgcttc
     5161 ttagccggct ttatctcgag agcatggcca ttcagctccg caccattcat tgctgcgatc
     5221 accttctgag cttcctctac aggcacttca acgaatgaat atttttcgag caaatgcaca
     5281 cgaccaatgg catcgagcga taggcctgta cgctctgcaa tcttatttct caggtcctga
     5341 ggttttaggt ggtgccgttt ccccatggag acaaaaagag atacagtctc cttcgatgga
     5401 agctctttca ttgaagatag tgggctggaa gagattgcgc ccgaaggcgc gcctttaatt
     5461 gattctgctg cggaaggagt ccccataaat aagcttttcc cctgttcgcg acttgccttt
     5521 tgaggatatg ttttttgggg cgatgttttt tgctgtggct ttgccctgga taggcctgcg
     5581 gcgcgaagaa tcatgagtgc tgcagcgtag gagcgcagat ggaaaggtat ttttcttctg
     5641 aatgctctgc gaacatcttc gaggagtttc gggtcctgcg gatgtctaag ctcatctagt
     5701 aggccatcga gaaaggcttt tagagctttt tcgtcaaatc tggaggctgc agcatcatta
     5761 ttgtcctgca cgaatcaact cctgctatat gatgaaataa tgtcttatat gcgctaatat
     5821 acttcagttt tcatcaccat gtaaactata gtattaaaaa gtatagacaa gaaagcctct
     5881 ctgcataaca atatgggaaa gttctacaag ctataaaacg atgaggagat atggagaaaa
     5941 acaacaggcc aaagatttgc ctctgtatga cgggaaagac aatcgaagat gacctgcgtg
     6001 tcctcgacca atatcgttct gtcgtagatt gtgtcgaatt acgcgctgat tgcttagatc
     6061 caagcgaacg tttcttaatt cgcgacttcc cagaaaaagc agggcttcca tgcatactga
     6121 cgataagacg tttaaaagat ggtggcaaat ttgaagatgg agaaggtgtt cgccttgttc
     6181 taatagcaaa ggcgttgtcc tttccaaagc cagatgctag cgctaattat gcctttgtcg
     6241 atcttgagga tgatttcagg gcaccagtcg tcgaagaagc ttgtcgcacc ttcggaactc
     6301 gcattattcg atctcaccat tacctcgatg gaatgccaga tagtctcgat gatgcttatg
     6361 aggagctgac tcttgaagaa gatgaaatac ccagactcaa cgtaaatccc cagaacagcg
     6421 aagattttat ccgcttttta gaatggtgcc ttcaaaaacc aaagactgat caactcctta
     6481 tagctagcgg aaactatggt atagcctcgc gcattctatc atggcgcctt ggttcgttat
     6541 ggacatacgc gagccctttg cgaagtggaa tggaagctgc cgcgccgggc catatcgatc
     6601 ccttagatct cataaatatc tacaatttcg accttgtcga tagcgatacg acaatttata
     6661 cactcatggg tcaaaactct atcgctcaat cgctctcgcc atacctgcac aataatgcat
     6721 tcagaaaaat gggtaagaat gctcttctta ttcctacacc tgtagacagc tgtatagatg
     6781 ggctaaagat actcgaaata cttggaggca aaggagcggc aatcacggtc ccattcaagc
     6841 aagatattct gccatatctt tctttccatt ctaccgatgt gcaactcata ggagcctgca
     6901 atacgcttgt gtggcgcgac tcgggatggg cgggctataa caccgatgcc gatggctttg
     6961 agcgcagtct tctggaattc cttggcaaac aggatatttc tggatgtaag gcgacaatta
     7021 ttggtgcagg aggtgcggct aaatcggtcg cactttctct gtttagaatg ggagcaagag
     7081 ggcttgttct caatagaacg tatagcatcg cccgcgatct agctcgaaaa tacaactttt
     7141 tatatggtca tctcgacgac agggccatag atctcatctc ggaacattcc gacttgattg
     7201 tacaagctac aagtgtcggt atggagaatg aaggcgatcc tatcagtttt tatgagttca
     7261 aaggctccga agctgtcttc gatttaatct attatcctgt taagaccttg tttctaaagc
     7321 gagccgaaga ggcaggatgt cgaaccatga atgggttcaa aatgctttgc taccaagcgg
     7381 ctggtcaata taagctctgg atgggagaac ctccacccgc gatctactct caactcgagc
     7441 agcccgagca attatagttc gattagtagt acataggtat cgaggtgggt aagaattcag
     7501 gattataaaa attctatagg aggcataaat gcccaagtct gcatttgtcg cgatcgttgg
     7561 caggccttct gtcggtaaat ctacactgct gaatgcacta tgtggagcaa aagtttctat
     7621 agtcagctcc gtcccccaga caacacgcaa tgcaatccgt ggcataataa ccaagcctca
     7681 aggacagctc atttttgtgg atacaccagg gtatcatcta tcggacaaaa aactcaatct
     7741 gaggcttcga gagatcgttt tgcaatccat atcggaagtg gatcttattc tctatctcat
     7801 agatgctacg cgcgagccgg gcaaggaaga ggaggcaatt gcagagcttc tttcgagaga
     7861 aatccatcga acagttgtgg ccattaataa gatcgatgat cccgaagcta ggcctttatt
     7921 gatcgaagag ttcgtgatac ctcgccttcg tggagcaaag cgcgtttcca tttctgctgt
     7981 cgaaaaaaca aatctcgaat ccttgatcga gatactgttt tctctcgccc ctgaagggcc
     8041 tttttggtat ccagaggata tctacaccga tcaggagcct gttttccgca tcggagagct
     8101 cattagagaa aaggctattc tcaatacata ccaggaatta cctcatgcta tcgcggtaga
     8161 atatcgcgaa tcatcaaaac gtgcagacgg agtcctcttt gcacgctttg atattcttgt
     8221 agagagagat tctcaaaaag gcatagtaat aggtagccag ggttcggtta taaaacgcat
     8281 ccgcgaagaa gccgaggctg atctcaaaga gctattcgat tatccaataa agctgcaaat
     8341 gcaagttagg gttgatcctg attggagaaa aagcgattcg agactttcgc gcataatctt
     8401 ttaatattta gtaattcgaa tgtcttggtt gcgcccattg cgctggcgta tctataccgc
     8461 atcatagggg caaaaccttc ttatatcgga tatcgaatcc ttctattccc tcagaaaaga
     8521 tttcataaaa agttttctct gccagcggaa ataccttctt gttaagaact tcgagctgtt
     8581 ccggatcgaa gcgcgatagc acatatcctg caacatcatc gtgttcaggt cttccaatgc
     8641 ctaaccgaag cctccagaaa tcccttgttc ctaagtgctt ctccaacgag cgcaagccat
     8701 tatgtccgcc aaggccgcca gaaaatttgt agccaaaagc accaaagggc atttcgagct
     8761 cgtcatggac aatgagaagt tcatctatcc ctaaaccata aaaatgaaca gcttcaccaa
     8821 cgctctcgcc cgatagattc ataaaagtat gcggtttcaa gaaatatatt atagttgaac
     8881 ccaatttcat ttgagaaaac tgccctttga actttgcact ccagcatagt tccgaggcta
     8941 tgatcatcga atcgagaaaa agccaagcta cattatgcct atggaaacga tattcctgtc
     9001 cctgattccc aaggaataca cataatttta tcatgatatg ctctttcttt gcgggaagcc
     9061 atcgaaaggc cccttaaatt tcataattgt atgcttgcca gaacctggcc catggcagtc
     9121 agaaccatag gtgaagaaga agccctgtgg ttttgctatt tctcttagct gttcgttgaa
     9181 cctcgaactc ttgaattcgc cgttgtacca gtagagctcg accccgtcaa ctcctgcatg
     9241 cctaaaccat gcaagaagaa gtcgcaattt ttctgcctct ttttgaaaca ggggaagttc
     9301 atcatcccag aaatgtccaa gatgaggaat caccacaaaa cctccgtccc catgcactgc
     9361 cttaaccaca gttgcaacct ctggtttgtc aagcttgaat tttttaagaa gcccgcctac
     9421 aaaaaatttc ttcttgaatt ccttgtaatt catctctggc ttaatgcagc gatgtgcctt
     9481 caaataaagg aacaaatcta ccttgctcca ggaaatcttg cgagcgagtt cttcatcgaa
     9541 atcaaggccc gtcttatcct taaaaagatc cttaaaagaa agctccttgt ctggatatag
     9601 ttctttagcc gattcgatga agaattctag ccttgatctt cgctgctcga gcgctttttg
     9661 aaggatacac ttggtcgaga gaatcgaaga attgaaatct gcttccggga aataggccaa
     9721 gagctcgctc ctgtactcaa tctgtggctc atatatgtct atttcgcaag ctggaattgc
     9781 ctttagcgat cttgtttcgc aggctcggat aaatcgctcc gtgccaccca tagtatcgtg
     9841 gtcggtcaga gccacaatat caagaccagc ccgagctgct tccaaggcaa tatcttctgg
     9901 ccaggacatt ccatctgaaa agcgggagtg cagatgcaaa ttcgagagca tttgacgcta
     9961 gtatacaata ttggaatttg cccttccata gataaaggca tatcgattag aataaaacta
    10021 tgtttcaact gaccgatgcc atactggagc gaattatctt tgccatggag gatcaatcga
    10081 aaaaattact catagacctg gaaacagggg atgtcataga caagactact atcgagaacg
    10141 aagattcacc cgagctatat tccgaaatgg cccagagatt tgtggaacca ccttcatggg
    10201 attcccgccg cggtttttct attctcgaag cttttgcttc aacggttacc tcgccaaccg
    10261 atatcaagat ggagctaaat gctgcgctaa gaaggggcaa aggagtcttc aaacaattcc
    10321 gtcaggtact atctaaagac caggatatct tcaagcgtta tcaggaattc aagcttgcgg
    10381 ccatgcgtcc cattgtggag agatggctag aagcactcca ggagggagaa aaactaacag
    10441 ctctaaagga ggagccggag gatctccgag atataatcgc ttccgatatc gacttcattg
    10501 tatctagtct ggatggaatt gttttcaaac tagaatccta tatcaattat tgtatagaag
    10561 aatccttcgg atccttgccc atagctatca agatgagatc cctttcggac ttgttggagc
    10621 ttgtaaaaac agatagtaac tcagtgcata tcgccatggg aacgatcgat ggtgtatcac
    10681 ctttgatagc aggcctgttt tcgcttcaag cttataaggg ctcacctatt tgcgttgtac
    10741 acggtgtatt tggaacaaaa gaaagtctta tgctggattt agaatggcct attatcgaat
    10801 gcatttctga atattgcacc aagatcggag caacacaaat tttgctcgaa gggcctttct
    10861 tttcctcatt attatgcgat agtgccctcg agtatggatt ttctcaatca ggcaccattt
    10921 tccataagaa tctttgacaa ttgtgacaaa tttggtaaaa atgtaaaata taagcctgtg
    10981 aagagatagc caagaaatat ggctaagaaa ataggccaag agccacaatg gcggatgtca
    11041 agcaagatat actgcccaat aggaggactt atgaaaaact gctttctttt cccaggccag
    11101 ggagcacaat accctggaat ggccaaagat ttctatgaag caagtaaggc tgtccgcgat
    11161 ctgttcaaag aggcctcgga agctgcttcc atggatctaa agagactcat cttcgaaggc
    11221 gacgaagaaa cccttaggca gacaagaaac acacagattt ctatggtcgt agccgaggca
    11281 gcttcggctc tcatggccag agaacagggc ataacacctg ccggggcagc tggtttctcg
    11341 gttggcgaat ggccagccct tgccgaggca aaagtcatta ccaactacga catgtttcgt
    11401 cttgtgtcag aacgcgggag gctaatggaa gaagctggca atcgtgcagg cggcaccgcg
    11461 atgagtgcgg tgctatatct ctctcctgag aaaatagaac aggtaataca tgaagcgggc
    11521 cttaaacaat gttggatagc gaattacaat agtcccgtcc agtgcgttat ttctggaacc
    11581 gaaacggata ttgtcattgc ggaagagaaa ttgcggcagg caggggccaa gcgtgtcata
    11641 aggctaaagg tatcgggcgc atttcattct ccgcttatgc aggatgcata cgatcctttt
    11701 aaggaacttg ttgaggctac tcaattctca gatccgaaaa tcaacctcta ttccaatgtt
    11761 acaggcaaga agatgctaag cggcgaagaa gcaaaaaaaa atgcatcgct tcagatcata
    11821 tctccagtac gctggatcga tgaagagagg aatatagctg cggattgcta tgactattgt
    11881 tttgagacag ggccaggaac agttcttgcg ggtctctgga aagcctcggg gtccactatt
    11941 ccatgtatcg cagctggtac tttgcaagcc cttgccgcct tttctctata aagtatcgag
    12001 tctcttattt cgatactttt gtaatctcga aactctagta aagcataagg agcaaatatt
    12061 atggagggcg ttctatcggg ccaaatttgt atcgtaacag gtgcttctcg aggcataggc
    12121 cttgccatag ccactcgatt caagcaagaa ggcgccatag tctatggatt gtcgcgcaca
    12181 aaagcagaag cggatataga atggatttca tgcgatgtca ctgatgaatt atctgtggaa
    12241 tccgctatcg atcaaatcct caaaaaagaa aatcgcatcg atatattggt aaacaatgct
    12301 ggcataacaa gagacggcct ccttatgagg atgaaaaccg aagactggga agctgtattg
    12361 aattccaatc ttcgaagcgc tttttttctt agccgagcag tttcgcgtat catgctaaaa
    12421 cagcgctcag gctgtatcct caacatgtcg agcgtagtag gcctccatgg taatggaggc
    12481 caagccaact atgccgcctc taaagccggc ctaataggat ttactaagag ccttgccaaa
    12541 gagcttggat ctcgcaatat acgcgtaaat gcaattgcgc ccggctacgt ggagacatcc
    12601 atgactcagg tgctgccaga tacggccaaa gaaaatttaa aatcttccat tcctctcggc
    12661 cgacccggca ctcccgggga tatcgcagag gctgccctct ttctctgtag cagctacgca
    12721 gcgtatatta caggagttgt gttaaatgtc gatggtggca tggggatgta attatgagta
    12781 aaagaaaagt ggttattaca ggccttggcc tggttagccc gctgggaaat gatgtaccta
    12841 cattttggca gaatgtcaaa gcgaatagat ctggcgtagg gccgattaca ctgatcgact
    12901 catcggatct ggcggttaag atagcgggcg aggtcaaaaa tttcgatcct tcaaaaaaaa
    12961 tagacccaaa agatgcaaaa aaaatggacc gattcgctca atttgccgtc tatgcagctc
    13021 tcgaagcatt ggagtcttcc aatcttccaa aagaatcgtt ggatccaata agaacgggcg
    13081 tctgtctagg aacgggccaa ggcggcacca acaccataga agaatctgtc atacgtctca
    13141 cggaaaaagg gcccaacagg gtccctccaa tgactgtcgc aaagggtctt gcaaactttg
    13201 gtgccgcaca gatagctcta tccttaggag tacatgggcc caatatgacc gtggtcacag
    13261 cctgtgctgc agcaacagac gcaatcgggc aggcgatgca tctaatcaga gatggccatg
    13321 cagatattat gtttacagga ggatcggaag ctagcatagc tcgtatgtgt atggctagct
    13381 ttataaatat tcaagctctt tcgatccgaa acgacgaacc agataaagca tcgcggccat
    13441 tcgatagaga tcgcgatggc tttgtaatgt ccgaaggcgc cggaattctc gtgctcgaag
    13501 aatacgagca tgccaaagct cgcggagcaa aaatatatgc tgagatagcg ggcttcggct
    13561 ctacctgtga tgcccatcat cttacagctc cagaccctga aggtctttgg gtttcaaagg
    13621 caatcggaat tgcgctcaac gacgcacagc tgaagcccga ggatattgat tatgttagcg
    13681 ctcatggaac ctccacgcct cttaacgatc ccattgagac aaaagccata aagaatgcat
    13741 tcggtgacca tgcctataag ctaaagattt ccagcctaaa atccatgata gggcactgca
    13801 ttggcgctgc tggtgcaata gagaccattg cagctattct tggtatgaat gagaattatg
    13861 tgcattccac aataaatctc gataatccag accccgagtg cgacctcgac tatgtgccta
    13921 acaaaggaat aaacatgcct gttagagcct ttgtcaagaa ttcgatgggt tttggtggac
    13981 aaaatgcagt gcttgttata acaaaaccat gaactatatt tcgggatccc tccctcgaaa
    14041 aatatctctg atgcctggaa atccggggct cttaggtgct attcccaagg cagagggagt
    14101 atacttttcg atattttcaa gaaatgccac gggcatgata ctctgcatat atgaaaaacc
    14161 ccacgacgat gtccctgtag cttctctaca tctagaccca ataagaaatc gtacaggtga
    14221 tgtctggcac tgctttattc ctgggcttaa gtcggaggtt ctgtatctat ggcgtgcaga
    14281 aggcccattt gctccacagg aaggccatcg cttcaatcca tataaggtcc ttatagaccc
    14341 ttatgctaaa gcaattaccg atggttctct ggaactctgc tccgctcttg cctacgatga
    14401 aaattcacca gagaaagacc tctctatttc gactactcca aacgatggtt ttatgccaaa
    14461 gtgcgttgtt gtagaagatg aatacaattg ggaaaatgac acccccttaa actatccgct
    14521 taaagattgc atcatatacg agactcatgt acgaggattg acaaagtctg ttacagcaaa
    14581 agtaaaacat catgggactt atcgcggagt agtcgagatg atcccatatt tcaagagtct
    14641 tggcattaca agcctagaac tgcttccagt acaggaattc gactcttccg agcgctttag
    14701 gcgaaacccc atcacgggcg agaagctttc gaactattgg gggtattctc caatcgcttt
    14761 ctttgctcca gagagttctt acgtcttcga tgaaggtgtg gaccacgaca gctattgccc
    14821 ctcctatcct gtcaaagaat tcaagtacat ggtaagagag ctccacaagg ctggcatcga
    14881 agtcatccta gatgtagttt tcaatcatac cgccgaaggc aatgagctag gtccaacttt
    14941 gagtttccgt ggactagata attctattta ttacatgttg gcagaggaca agcgctatta
    15001 tcgcaattac tcgggttgcg gaaacaccct caattgcaat catcccgttc tacgtacctt
    15061 tattatggat tgcctccatt attgggtagt taccatgcat gtagatgggt tccgattcga
    15121 tctcggttcg attttgggac gggattcaca agggaatctg ctagccaatc cgccagtaat
    15181 agaatctata gcggaagacc cgatactgcg agatacgaag atcattgccg aagcctggga
    15241 tgctggcggt gcttaccaag tcggggcttt ccctggtgga cgatgggcag agtggaatga
    15301 ccgatatcgc gatgatgtga gacgattttg gagaggagat gaagggttta ctccgctcct
    15361 agcaacaagg attactggat cgagcgacct ttacctcaag aatggaaaac gaccatttca
    15421 ttctataaac tttgtcacct cgcacgatgg ctttacaatg aatgatctcg tatcctatag
    15481 ccgcaagcat aatgaggcga atggagaaaa caactgcgac gggattgcgg agaattattc
    15541 ctcaaactat ggcacagaag gcccttcaaa cgctcctttt atcgaagaaa ctcgaaatcg
    15601 acaggtaaaa aatttctttg caacgctctt gctttcaata ggcacgccca tgctcctcgg
    15661 tggcgacgaa tttcgtcgaa cgcaaaatgg caataacaat gcctactgcc atgacaacga
    15721 actcacttgg tacgactgga gcctactaaa aaagcataaa gacatttatc gtttcgttaa
    15781 gtgtctcatt gcttttcgca agagccacaa tgcattcaga agagaggact tttttagagg
    15841 tgtgggaaaa cgcagtattg gcactatcgg cgcttccggg caatccaaaa taaataaata
    15901 caaaccaaac gacatatcag atataggaac tctccccgat atcatctggt atgatcaaaa
    15961 cggcctacct ccacagtgga acaagatggg caaagtgctt gttgcgcgta taaacgagcg
    16021 tatctcatcg ggagattttc cttgctctta tcttctagcc ttcaatgcat ctcctgaagc
    16081 tgttcagatt tgcctccctc atgataatga acaggacatc tggaaaagag taatagatac
    16141 ctctttccct ccaccacatg aaattgaaga gaaagaccaa cctgtcctat cagaccaaca
    16201 gaagtatctc atgtcaccaa gatcttttat tttgctgatt tcgatatgcg aacttgacga
    16261 gaatcagagc ttgccttcat gaaatttcat gaatatctta taacacgtga gaacatcggc
    16321 agataatgaa aaagctaatt tatatttaag aagaggcctc cgcgagcttt ccagacacaa
    16381 gccagcacaa gctctcgaat tatttcgaaa gtcgatcgaa ctcactccgc cctcctctga
    16441 aaagacactt tcaagggcgc tttactggtt gtcaattgct cttctgcagc tgaataagcg
    16501 cgatctagca gtaaaaagcc ttgcaaatgc ccaaaaaata catagacagg gctatattcg
    16561 cagattctat atacgccatg tcaatgaata tggcatgatc aaacagccaa caaaagagct
    16621 agatgatcta tatgcttttc taagtattca gctttctttt tatttgctca atcgtccaag
    16681 ctatcgtttt ggctctgagg ccgagcactc gatggtgctt gcttttctgc tacatacttg
    16741 gaagaatatc aaaggcaccg aagagtttaa atctctcgat tgtagcgaaa aattattgct
    16801 tttcaataaa cttaagattg attttcccgc tttcgctcca tattctgtcg tacaaagaaa
    16861 gagagaacgc caaattatac catcaaatat agcctataac caactttgct cctgcggatc
    16921 tggcttgccg ttcatgcagt gttgtgggag gatccgggga atttccgagc tcttaaagtg
    16981 atcgaatgtt tcaaattgac acaataccga ttgctttaag gtttagtata tcaaaacgat
    17041 acatattcgc catattcttt agcaaacagc tcctttttta gaaaagccag cacgtcttgc
    17101 cgtatgctaa tttcatttat ctaaatgaat tgctgatttg tcaatgaatt ggcacgcttt
    17161 ttgcttccta aattagcaag gagatcttga tgacgcaaaa caagaaactg aaggaaacac
    17221 gttctagtgc gaattatgac gacgagaatg tgttgtccat gtatctccgc gagataaaca
    17281 gaattccgct tctttctcgc gaagaagaaa atgaatacgc gactcgtgca gccgcaggcg
    17341 acgaaaaggc aaaagagata ttggcgaaaa gcaatctcag atttgtcgtt aatgtcgcta
    17401 aaaagtacca gaaccaaggc ttgcctcttg cagatcttat agcggaaggt aacataggac
    17461 ttctgagcgc tatcgatcat tatgatgtta caaaaggcta tcattttata tctta
//
LOCUS       DAOSOB010000041        17337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3301_length_17337_cov_74.965398, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000041 DAOSOB010000000
VERSION     DAOSOB010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 17337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17337
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3301_length_17337_cov_74.965398"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..371)
                     /locus_tag="PLS88_05640"
     CDS             complement(210..371)
                     /locus_tag="PLS88_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOU06856.1"
                     /translation="MKKYVCDVCGYIYDPAEGDPDGKIPAGTPFEELPDDWVCPMCGA
                     SKDSFSPMD"
     gene            complement(399..773)
                     /locus_tag="PLS88_05645"
     CDS             complement(399..773)
                     /locus_tag="PLS88_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOU06857.1"
                     /translation="MTERKQIYKCMKCGNIVEVLHEGQGQLVCCGEPMKLMVENTSDG
                     AKEKHVPVLEKTADGWWVMVGSILHPMEEKHYIEWIELIADGHSYRAYLSPGQEPKVF
                     FPVKAEKVEAREYCNLHGLWKA"
     gene            complement(792..1367)
                     /locus_tag="PLS88_05650"
     CDS             complement(792..1367)
                     /locus_tag="PLS88_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOU06858.1"
                     /translation="MKSIKGTKTEVNLLTGFAGESQARNRYTYFASKAKEEGFIQIQL
                     IFEETANQEKEHAKRLFKFLEGGTAKVSAEFPAGVIGTTRENLAEAAGGENYEWQEMY
                     PSFAKVARDEGFEAIASVFDSIAVAEKQHAKRYEALMANIDEGQVFKRDKPVVWRCLN
                     CGYVYEGTEPPKACPACAHPQAYFELLAENW"
     gene            1625..2332
                     /gene="radC"
                     /locus_tag="PLS88_05655"
     CDS             1625..2332
                     /gene="radC"
                     /locus_tag="PLS88_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOU06859.1"
                     /translation="MTEWQAYEKAELKIEPNTPSLDIRERMLRFGSGSISNLDLVVAI
                     LGSGVPGKPVRRLAKEVLNQITKNSDSCDLDELMLISGMGEAKSCAVAAALELGRRIF
                     STRGTRIGMPKDAYPLLIHFADIRQEHFIVISLNGGHEVNAIREVTKGLINKTVVHPR
                     EVFADPITDRACAIIVAHNHPSGNLEPSDEDVDITKRLRQSGDILGIPMLDHLIFSES
                     GYFSFMEHGLIVTAKGD"
     gene            complement(2367..3545)
                     /locus_tag="PLS88_05660"
     CDS             complement(2367..3545)
                     /locus_tag="PLS88_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOU06860.1"
                     /translation="MDIFQKCFDFDLDRQAMEKGIYPYFQPLDGLEGTEAVFNGRKLI
                     MIGSNNYLGLTMHPKVKEAARKGLEEYGPSCTGSRFLNGTLKLHNELEERLAAFVGKD
                     AALVFSTGMQTNLGAISSLVDRNDIVIADKDDHASIVDGCRLSMGEMKRYIHADLDHL
                     ERILQKVPSESGAMVIVDGVFSMGGDIADLPGLVKLCEKYGARLYVDDAHSIGVLGDG
                     HGTAWHFGLTDKVDIIMGTFSKSFASLGGFVAGHRDVINYIKHTARSFMFSASLPAPN
                     AMAALAALDIMETEPEHVQRLWDNANFMMKGFRELGFNIGKTQTPIIPVIIGEDEVCF
                     RFWKELFDNGLYTNPVISPAVPEGMALLRTSYMATHTKDQLQRALDIFEKAGKKFGII
                     "
     gene            complement(3788..4555)
                     /locus_tag="PLS88_05665"
     CDS             complement(3788..4555)
                     /locus_tag="PLS88_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOU06861.1"
                     /translation="MKHFAFIGLGPFAMSMLERISVISDQIIAADENPDRIEHVKEMV
                     SVAFTMNLLDEEAFERVFHEPVDVAIVDIESSGPELLLVTFRLKKLGVPEIIVKSNSE
                     EYEELLRLVGATRVVNSDREAALRVTPLVLSASLTNFMPISGDLVLAEVIAPDFVVGK
                     SVIEGDLRNTRHINVIAVKYASEMDSSGTHKGVFHNLDTKYRFKSGDVLLVTGKESDV
                     FEFSGIPETIEREKEATKMTSFLKTMFNRKKKDIANK"
     gene            complement(4582..5901)
                     /locus_tag="PLS88_05670"
     CDS             complement(4582..5901)
                     /locus_tag="PLS88_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOU06862.1"
                     /translation="MSSRIGKEKLIPLLYFLGTILAGSLLLVLPISSKEGSLSYLDAL
                     FEATSAVCVTGLAVIDTTKLSRFGQTVIILLIQLGGLGIVTFSTLYIFMPRRRVSVTT
                     HGMIRDYTLSSVEYRTRRTIREIVGWTFGFELIASLVIWPTLARNGYSFYDAIFHSIS
                     AFCNAGFSTLQSGMESFRDNVILNVVTMLLIVFGGIGFIVLQDMARFFKKEKRHLSYH
                     SLVVLRTTSALIVLGAIVFYLLEHNHAYKEMSQGSKIMAAFFQSITSRTCGFDTVPQA
                     NLSGSSQLATIILMFIGASPGSTGGGIKTVTLYILILVAFRMREGGDTIIDRYRTVTP
                     NTVFKAITALVRGAIIVLFATAILLVAERASGHTIGLETAVFECVSAFGTVGLSIGIT
                     PYLTAVSKLVLIAAMFMGRAGLFVLAIPPSKYSPEPFAKYPRADVLL"
     gene            6114..7526
                     /locus_tag="PLS88_05675"
     CDS             6114..7526
                     /locus_tag="PLS88_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOU06863.1"
                     /translation="MKIVILGAGLLGSLIASELIAENKDVVIIEKNSNTARSIANELD
                     CIVEEGDGENIETLKAAGVADADWFIACTGSDETNIVSCGFVAENFKNVQTIARARNP
                     YFASFKNTGKKILGVDYIINPEAETAESIARIIFRGMSPEIIDVKEAGIQLRRILCKK
                     EPRFVHESLADIRARIGQDFMVPAVLREEELTVPNGDFVFEEEDAAYILGEPSILDKL
                     FGPCKDTLRKFSSIVILGAGTVTTLLLKELGMRQLSASAGSQRNKGHDKGALSLLGNP
                     KIKVIYDNRETLKTLSATFPDIEAINHQLSDEYLIKEENIGSADIVLCLTSEQSINIL
                     SALLAQDAGARRSLALVTNDLYSPLLYLLDINIVVNEKTVMSGAILDKVRKAKIRRLY
                     SFPHNEYELIEIQITSSFEQLGVEIRNMNLPKGFLIAFVIHGGKTYIPTGSTKLFEND
                     LVGLIIKKEHIGKLESVFGA"
     gene            7528..8985
                     /locus_tag="PLS88_05680"
     CDS             7528..8985
                     /locus_tag="PLS88_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOU06864.1"
                     /translation="MHFKSILRMLSIILLIVSIFSLACGLFSIVEGLSWRVSFSLFIP
                     TIISLAFFMLMYTITRHDERPFLTNKDSFLFVTFSWVFSSALGAIPFTFSGYIPSYID
                     AFYETVSGFTTTGASILTDIEAIPRPLLLWRATTHWLGGMGIVVLLVAILPSLGFSAI
                     RIMEAETPSPEVDRIAPRISKTAKTLWLIYCGFTICEIILLVLGGMPLFDAICHSFAT
                     MATGGFGTKNANIAYYKSGFIQWVITIFMFLAGMNFTLHYHFLKGRIGAVLRDTEFKV
                     YFAIVVISSLVIAVDLLVSGVFRNIEDSLRHASFQVSSILTTTGFASTDFGQWPQLSQ
                     TVLFLLMFIGGCSGSTGGGVKVIRLVVLFKMAVNEMRYVANPRGVYTIFMGDKALRKN
                     VIYDISGLVFLYFAFFFLSVFVISFSGVDVLTNITAVIANLGNIGPGLGKVGPAFNYG
                     FFPWWAKLWLSLTMLVGRLEVYTVLALFTRKFWRV"
     gene            complement(9131..11761)
                     /locus_tag="PLS88_05685"
     CDS             complement(9131..11761)
                     /locus_tag="PLS88_05685"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5054 domain-containing protein"
                     /protein_id="HOU06865.1"
                     /translation="MSNELKKVFIVFSTHFDNGFTGTMEDVQSDLAWNLIPRALDTIE
                     KSSDFGPNLRFTWTLPAWPLENALMRLEGTRLGKQLERVVAEGYITWHALPFTIHTEL
                     FGLEDFIRTMLPGYRLSRRFGRRSIAAKLTDVPGHTRILSTLMAASGVSFLYIGCNAC
                     STPPDVPILFDWEGPDHTRVTTMYSKGGYGSPILPPPGYEFPIWLALIHASDNGGPQP
                     AEAITEAVAQIEAAYPGIDVVVGSLDDFAIALRDLKLELPVVRSDLADSWIHGIATMP
                     EELGMIRQYRERLAAIESFEALKGLESKDRPPDGFSTMIHDSYEAISLFGEHTWGLDT
                     KLALNPPAEGGRVYDKERFTKLLEEGAWGRIRGSWREKAALADRFVAAVNSLEKSLEK
                     SDSYSLGSEKSRCLYSVRNHQLWEHNGLVRLGQMSDSAQVLVEGQLCPTVRLDGELWA
                     DIGPIAPLGLRNIEIVEKAEKPRAASQLIARKEGRKDVLDNGLIRVVVDAESGSISSF
                     FDLRSGREWVDAETREGFGSYRYDIFSRDEITAYLRDYAYDLEPWFVEDFGKPEYPKI
                     VHHTYLSSLENISITNGVDWGALKVVWSQEPESMDTYGNARRIIQKLTLPRGQPWLDI
                     TYHLEDKSATQFLESGHIVFPLAAQDPHYAINKTGWVLDPAHDIEKDANRILYACERW
                     IDVEDSGEGLLFMPLDSPLFSIGSIGIERFDGRALPGPPILNFNLFNNQWGTNFPQWI
                     EGSLTYRFRIAPHKGDWREAKAWRLAAEAFQPPACLPYHGASEISSLAAEVAGLGDLE
                     VLVWKRPNEGEGWMLRLRDPTGLGGNRKLRLSPPPRMVFACDLFENGGKPIAIKQDGG
                     SAIVEFGIPAFGIMTLKLLY"
     gene            complement(11758..12663)
                     /locus_tag="PLS88_05690"
     CDS             complement(11758..12663)
                     /locus_tag="PLS88_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU06866.1"
                     /translation="MSLEVSSYQQGSDHQVASSRRSASSRRAWGHALRTIIMLTLALI
                     WFIPAFGLFITSLRPQSDIATSGWWRIFAVPRFTFENYSIVLGSHNFSTNFLNSFIIT
                     IPSTILPIALGALAAYALACMNFRGRNFVFFSVIAILVLPLQTTWVPVLRMQSRFHMT
                     GSWISIWLAHTAYGLPFAIFLLRTFFAELPKELLEAARIDGHSEFSIFCKIVIPLSVP
                     AIASLGIFQFVWVWNDLMNALVLLQNPKKFPLTVALQGLLGQYGSEWNLLASGAFVSM
                     SIPLVVFFLLQRYFVRGLTAGAVKG"
     gene            complement(12660..13568)
                     /locus_tag="PLS88_05695"
     CDS             complement(12660..13568)
                     /locus_tag="PLS88_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU06867.1"
                     /translation="MKVKRDSIQGWLFLAPALILLVAYLVYPCLSTLKMSFYGGQGFS
                     PKRFVGFANYARLLGGGDSLFLNLKWPPSGALINNILWILFFAGGTVVIGLAIAVLAD
                     GLRLEKIFKSLIFLPMIISATAASVIFRFLYSPDPHIGLLNAFISSISPGKKPIPWLG
                     STDIVNIALIGAGIWIWTGLSMTVLSAAYKSLDREILEAAKVDGASSWTCFWRISLPM
                     LEDQIVFVAITMIINSLKLVDLVLVMTGGGPMGSSRTIGFSFYWEVFNNNLVGYGSAI
                     AMILLILLIPFMVFQVRRIKAQESYQ"
     gene            complement(13693..14985)
                     /locus_tag="PLS88_05700"
     CDS             complement(13693..14985)
                     /locus_tag="PLS88_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU06868.1"
                     /translation="MKRTLVILLMLVVAFGSWAQGKSATASTKPVTILATWGGDEEAG
                     FREVLDRFTAETGIPYSYEGNRDSTVVLKSRVASSNPPDIAILPRPGEVANYARQGVL
                     IPLNQGQKDDILPTSLLDANYGKAWIDLGTVDGKFYGLIVKANSKSTFWYKPVSFASL
                     GVKTPDTWEDLLKIADKYVAAGKKPLAIGAQDGWTLTDWFENIYVRTAGPDMYMKLHV
                     THEVKWTDPTVVTAIKRFIELVTPTDKKLAGGADGALSTGFIDGFNEVLRPNPAAEMY
                     YEGGFMSSFAAQNFPNLTPGKDYDFFDFPSIDSKWGKPVVGGGDLAVVFSNRPEVVSL
                     MRFLAGKEANTIWASAKKGAVVSPNKNVDLSVYPLLTAKEARQLTQATSFVFDGSDLA
                     PPAVGGDAEFVALQRILKNPDKYMEALNYLEGVASQCY"
     gene            complement(15109..16224)
                     /locus_tag="PLS88_05705"
     CDS             complement(15109..16224)
                     /locus_tag="PLS88_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU06869.1"
                     /translation="MNNDQGAEPMYRRIYDSILADISSGKLLPGDRVPSEKELSSRFS
                     VSRITSKHALELLAGNKIIERFPGKGSFVARPEIAAKRAKIGLLGKNIGLVMPDFSET
                     FGLGLLFGIEDRCRELGYSLILCRTLDRPELEEKAIEHLRQLNVDGIIVLPVHGENYN
                     PSVLRLVLERMPMSFVDRRLRGLAASFVGTNNIEAAKIGVSHLIDLGRQRIAFLSGPI
                     KDTSAIEERREGFVEALADYGLPLDTDLWMTYVDSFRYGIISCEQAKANLSQMREHLS
                     AHPDIDAVFSVEYEGAVLAREAARSLGLKVPEDIAIVCFDCPTNCYLPPAFTHLKQHE
                     YDIGQKALDIVHAQILGKGQGNAKVYIPADLIRGESS"
     gene            complement(16283..16603)
                     /locus_tag="PLS88_05710"
     CDS             complement(16283..16603)
                     /locus_tag="PLS88_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOU06870.1"
                     /translation="MEISLKPVLRAVIFDLDGVVTDTTRYHFQAWWRLGEEHGCWHVE
                     EELNEKLKGVGRMESLDIILKENAIDLPVSQKIKLVERKNLYYKEFLTRLTLEDILLG
                     MKSH"
     gene            complement(16989..>17337)
                     /locus_tag="PLS88_05715"
     CDS             complement(16989..>17337)
                     /locus_tag="PLS88_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06871.1"
                     /translation="ADGTAIRWKSRSSPGFFIPIPRSLIQSAQIYDLILMNTHTDGPS
                     IKELKDAKVTFVVIGSIEGLSVLQVDIDNVAAAKDMIEYFVSLGYRKIGMIAHAPFEF
                     YAAADRLEVYLQI"
BASE COUNT         4671 a         4349 c         3905 g         4412 t
ORIGIN      
        1 gagaaatcat atacgctcca agcccataaa aattaagata aaagaataat gcgatatata
       61 aaataaaaaa agagggagcg caaaccccct cttttttatt gtgccctctt ttttattgcc
      121 ccctgccgga aaaagcatcc gccgtcttat gatgcaaaaa gcctagatta caaaaatcat
      181 cggctaattt cagacaggtg gctctttttt caatccattg gcgaaaatga atccttgctc
      241 gcgccgcaca tcgggcagac ccagtcatct ggcaactctt caaatggggt cccggcaggt
      301 atctttccgt cagggtcgcc ttccgcagga tcataaatgt aaccacagac atcacataca
      361 tacttcttca tagtgcgctc ctttaaaatt tggctctctt atgccttcca caaaccgtga
      421 agattgcaat attctctagc ttccaccttt tccgccttta cagggaaaaa gaccttgggt
      481 tcttgcccag gcgaaaggta tgctcgatag gaatgtccgt cggcgataag ctcaatccat
      541 tctatgtaat gcttttcttc cattggatgt agtatcgaac caaccatcac ccaccagccg
      601 tcggcagttt tttcaagtac aggcacgtgt ttttcttttg cgccatcgct cgtgttctcc
      661 accattagtt tcatgggttc tccacagcac acgagctgac cctgcccctc atgaagcact
      721 tctacgatat taccgcactt catgcatttg taaatttgct ttctttctgt catataccat
      781 agcctccttt attaccaatt ctcagccaaa agctcgaagt aggcttgagg atgagcacat
      841 gcgggacatg ctttgggagg ttcggttccc tcgtagacat aaccgcagtt taggcaacgc
      901 caaacaacag gcttatcccg cttgaagacc tgcccttcat cgatattcgc cataagcgct
      961 tcatatctct tagcatgctg tttctcggcc acagcaatgg aatcaaatac gcttgcaatc
     1021 gcctcgaaac cctcatctcg cgccactttg gcaaagcttg gatacatctc ctgccactcg
     1081 taattctctc cgcctgctgc ctcggcgaga ttctcccgcg tggtgccgat tacacctgcc
     1141 ggaaattccg cagagacttt tgcggttcct ccttccagaa acttaaataa tcgctttgca
     1201 tgctccttct cttgatttgc cgtttcctca aaaataagct gtatctggat aaaaccttct
     1261 tcctttgcct tcgaagcgaa ataggtatag cgattgcgcg cctgagactc ccctgcaaat
     1321 ccagtaagaa gatttacttc tgtctttgtg ccctttatgc ttttcatcat atcctcctct
     1381 cgagacttga gatttaacaa aaaaaccaaa tacctttttt gattatattc tagctggcct
     1441 tgaaacgcaa atgctcgatc acacaaattt gttgtgcccc atcaatctac tgatgatgct
     1501 ctctgcggat tcttatgtgc atgctgctct atagtgctcc ttaagctcta tctaaatgct
     1561 aattgaaccg aaatatattg aatcgagaac cactctcgat gcgtcataat cattaggaga
     1621 cacaatgact gaatggcaag catatgaaaa agcagaactt aaaatagaac ctaatacccc
     1681 atctctcgac ataagagaga gaatgctgag gttcggcagc ggctcgatat ctaacctcga
     1741 ccttgtggtg gcaattctgg gttctggagt tcccggcaag cctgttcgca ggcttgccaa
     1801 agaggtgctg aaccagatca cgaaaaacag cgattcatgc gatctcgatg aacttatgct
     1861 tatatctgga atgggagaag caaagagttg cgccgtggct gcagcattgg agcttggtag
     1921 acggattttt tcgactcgcg gcacgcgcat aggaatgccc aaagatgcct accctctcct
     1981 aatccatttt gcggatataa gacaagagca tttcattgtt atatcgttaa atggtggaca
     2041 cgaagtcaat gctattcgcg aggtaaccaa gggccttatc aataaaaccg tagtgcaccc
     2101 gcgcgaagtc tttgcagatc ccattacaga tagagcctgc gccataatcg tggcgcacaa
     2161 tcatccaagt ggaaatctgg agccttcgga cgaagatgta gatataacca aacgacttcg
     2221 gcaatcaggc gatatcctag gcatacccat gctggatcat ctcattttct ccgagtctgg
     2281 ctatttcagt tttatggaac atggactcat agtgacagcc aaaggagatt aggcgaccca
     2341 gcaagtggag tttcttatct cattgcctaa ataataccga acttcttgcc ggctttctca
     2401 aaaatatcga gcgcgcgctg cagttgatct ttagtatgag tcgccatata ggaagtacgc
     2461 aataatgcca ttccttccgg tacagctggg cttattacag gattggtata tagcccatta
     2521 tcgaagagct ccttccaaaa acggaagcat acttcatcct caccgataat tacggggata
     2581 attggcgtct gagtttttcc gatattgaaa ccgagctccc ggaagccttt catcataaaa
     2641 ttggcattgt cccaaaggcg ttgtacatgc tcaggttctg tctccatgat atcgagagcg
     2701 gcaagagccg ccatggcatt gggtgcaggc aaggacgcgg aaaacataaa agagcgggct
     2761 gtatgcttta tgtagtttat tacatctcta tgaccagcca cgaatccacc tagcgatgca
     2821 aagctcttcg aaaaggtgcc cattatgata tcgactttat cggtaagacc aaaatgccaa
     2881 gccgtaccgt gtccatcgcc cagaactcca atggaatgtg catcgtctac atagagccgc
     2941 gctccatatt tttcgcagag ttttactaga cccggcaaat ccgcaatatc tcctcccatg
     3001 gagaaaacac catctacaat gaccattgct cctgattcgc taggaacttt ttgtaaaatt
     3061 cgttcgagat ggtctaagtc ggcatgtatg tagcgcttca tttctcccat gctgagccta
     3121 cagccatcca ctatagaggc atgatcgtct ttatctgcga tgacgatatc gttgcgatcc
     3181 accagcgatg aaatcgcccc aaggttggtc tgcatgccag tagaaaaaac cagtgccgca
     3241 tctttcccga caaaagcggc gaggcgctct tcgagctcat tgtgaagttt gagagtacca
     3301 ttgagaaaac ggcttccggt acaggaagga ccatattctt ccagaccctt gcgagcggct
     3361 tccttgacct ttggatgcat agtcaggcca agataattgt tggaaccgat catgatcagc
     3421 ttgcggccat taaagacagc ctcggttccc tcgagcccat caagcggctg gaaataagga
     3481 tatatccctt tttccatggc ttgcctgtcg agatcgaaat caaagcactt ttgaaaaatg
     3541 tccataggga cctccattgc gcgcgcagat actttctgca acgcagtttg ccggacgaca
     3601 atttgccgcc ggagcgcaca ggctgtatat caagcctgag ccgcttcttt attgtaatgg
     3661 ttcgagaaaa tggaatcaac gagctctcca gtcaccgtga tgtggattac tgcgctcgaa
     3721 gatatgttgt ctagctatct ttgcactaaa ttgtcgtaca accgccctcc agcaatgcca
     3781 aaggccgcta tttatttgca atgtcttttt tcttgcggtt aaacatggtt tttaagaagc
     3841 ttgtcatttt tgtcgcttct ttttcacgtt ctatcgtctc tggaataccc gaaaactcga
     3901 aaacatcgct ctctttaccc gttacaagga gcacgtctcc actcttaaag cgatactttg
     3961 tatcgagatt atgaaaaact cctttgtgag ttcctgagga atccatctcc gaagcatatt
     4021 ttacagcgat aacattgata tggcgcgtat tgcgtagatc gccttcgatg acgctcttgc
     4081 ccaccacgaa atcgggagct atcacttccg ccagaacaag gtccccgctt atgggcataa
     4141 agtttgtaag agaagcggaa agtacaagcg gagttactct taaggcagct tctctgtccg
     4201 agtttactac acgggtcgcc ccaactagcc gcaaaagctc ttcatattcc tcagaattcg
     4261 attttacgat gatttcgggc acacccaatt tcttgagcct aaaagtcacc agcaagagct
     4321 cggggccgct cgattctata tcgacgatcg ctacatccac cggctcgtgg aatacccttt
     4381 cgaatgcctc ttcgtcgagc agattcatgg taaaagccac cgacaccatc tctttgacat
     4441 gctcgatacg atctggattc tcgtctgcgg caataatctg gtcgctgatt accgaaatcc
     4501 gctccagcat agacatcgca aatggtccga gaccaataaa tgcaaagtgt ttcatattat
     4561 accttcctta aaaacagcaa atcataacag cacatctgcc ctaggatatt tggcaaaagg
     4621 ctccggcgaa tatttagaag gtggaattgc gagcacaaat aagccagccc tacccataaa
     4681 catcgccgca atgagtacaa gctttgaaac agccgtaagg tatggagtta tgcctatgga
     4741 tagaccaacg gttccaaatg cggagacgca ctcaaaaaca gcagtctcga gccctatggt
     4801 gtgaccactc gctctttcgg cgacaagaag aatggcagtt gcaaacaaaa ctataatagc
     4861 gcccctcaca agcgccgtaa ttgccttgaa tacggtattg ggagtcacgg ttctatatct
     4921 gtcgataatc gtatctcccc cttctctcat cctaaaggct acaagaatca atatatacag
     4981 cgttacagtt ttaatacctc cgccggtcga gccaggcgaa gcacctatga acataaggat
     5041 tattgttgca agctggctcg aacccgagag attcgcctgc ggcacggtat cgaaaccgca
     5101 tgttctcgaa gttatggatt gaaaaaaggc cgccataatc ttcgaaccct ggctcatttc
     5161 cttatatgcg tgattatgct ccagtaaata gaagacgatc gcccccagca ctataagcgc
     5221 ggacgtcgtt cgtaagacaa ccaaagaatg ataggaaaga tgtcgtttct ctttcttaaa
     5281 aaatcgcgcc atatcctgaa ggactataaa acctatccct ccaaagacta tgagaagcat
     5341 tgtaaccaca ttcaagataa cattatcgcg aaaactctcc atgcccgatt gtaaagtcga
     5401 aaatccagca ttgcagaacg ccgatataga atgaaaaata gcgtcataaa aagagtatcc
     5461 attcctagcc agcgtaggcc atattacaag cgaagctata agctcaaaac caaaagtcca
     5521 tccaacgatc tcccgtatcg ttctacgcgt gcgatattcc accgaggaaa gagtataatc
     5581 ccttatcata ccatgagttg ttaccgacac ccttcgcctg ggcataaaaa tatacaaggt
     5641 agaaaatgta acaatcccca agccccctag ctgaatcaag agaatgataa ccgtttggcc
     5701 aaagcgcgaa agcttcgtcg tatctattac ggctaggccg gtaacgcaga ccgcgctggt
     5761 cgcttcgaaa agagcatcca gatatgagag gctgccctcc ttgctcgata taggcaagac
     5821 caaaagaagg cttccagcta gaatagtgcc caaaaaatat aagagaggaa tcagtttttc
     5881 tttccctatg cggctgctca actgaggctc ctgtcacagt ctgtcacaat atagtaggca
     5941 aaataaagat gaggctcaat ataccaacgt cgaggcgcct atttgtcggt gctttcgttt
     6001 tagagggcac acaatatagg aacgcctatc tatggggcgc gccactgcga ggctcatcat
     6061 cgtcagagcc atgtcattgt cagaatagcg ctcgctcgca tatacttcta tcgatgaaaa
     6121 tagtaattct gggcgctggc ttgctcggct ctcttatagc cagcgagctc atagcagaaa
     6181 acaaagatgt cgttatcata gaaaaaaatt cgaatacagc gagatccatc gccaacgaac
     6241 tggactgtat cgtcgaagaa ggcgatggcg aaaacatcga aacgcttaag gccgcaggcg
     6301 tagcggatgc agactggttt atcgcctgca ctggcagcga cgagaccaat atagtctcct
     6361 gcggattcgt cgccgaaaac ttcaaaaacg tacaaaccat tgctcgagcg cgcaatccat
     6421 actttgcgtc ctttaagaat actggaaaga aaatcctggg cgtcgattat atcattaacc
     6481 ccgaggccga aaccgccgaa agcattgccc gcataatttt ccgcggtatg tcgcccgaaa
     6541 tcatcgacgt aaaggaagcg ggaatccaac tcagacgaat tctatgcaag aaagagccaa
     6601 gatttgtcca cgaatcgctg gccgacatcc gtgctcgcat tgggcaagat tttatggtgc
     6661 cagccgtgct gcgcgaagaa gaattgacag ttccaaacgg cgatttcgtt ttcgaagaag
     6721 aagatgcggc ctatattctc ggcgagccat ctatcctcga caagctcttt ggcccatgca
     6781 aagacacctt gagaaaattc agctctatag ttatccttgg tgcaggcact gttactactc
     6841 tattactgaa agagctcgga atgaggcagc tctctgcttc tgccggttcc cagcgcaata
     6901 aaggacacga taaaggcgcc ctctccttgc ttggaaatcc aaaaatcaag gtaatctatg
     6961 acaatcgaga aaccttaaag actctctcgg ccacttttcc ggatatagaa gccataaacc
     7021 atcagttgag cgatgaatat ctaataaagg aagaaaacat cggaagtgcc gatattgttc
     7081 tatgccttac atcggagcag agcattaata ttctaagtgc tcttctagcc caagatgcag
     7141 gggcaaggcg cagcctggcg ctcgtaacaa atgatctcta cagccctctt ctctatttgc
     7201 tcgatataaa catagtcgtc aacgaaaaaa cagttatgtc gggcgcaata ctcgacaagg
     7261 taagaaaagc caaaatccga aggctctata gcttccccca taatgaatac gagctcatcg
     7321 agattcagat tactagttct ttcgagcaac ttggagtaga gatacgcaat atgaatcttc
     7381 caaaaggatt tcttattgcc tttgttatcc acggaggaaa gacatatatc cctacgggct
     7441 ctaccaagct gttcgaaaat gatctcgtgg gcctaattat taaaaaagag cacataggca
     7501 agctagaaag cgtttttgga gcatgatatg catttcaaat cgattctccg aatgctttcc
     7561 atcattcttt tgattgtctc tattttcagc ttagcttgcg gacttttctc tattgtcgaa
     7621 ggcctgtcct ggcgcgttag tttctcgcta tttataccga ccataatcag tctggccttt
     7681 tttatgctga tgtatacaat tacaaggcat gatgaaaggc cttttcttac aaacaaagat
     7741 agttttctct ttgtaacctt tagctgggtg ttttcttcgg ccttaggcgc tattcctttt
     7801 accttctccg gatatatccc ttcttatata gacgcattct atgagaccgt atctggcttt
     7861 accacgacag gtgcgagcat attgacagat atcgaagcaa tacctcgacc attgttgctc
     7921 tggcgagcga caacgcactg gctcggcggt atgggaattg ttgtcctttt ggttgcgatt
     7981 ctacctagct tgggctttag cgcaatccga attatggaag cggaaactcc cagcccagaa
     8041 gtggatagaa ttgccccgcg catctccaaa acagcaaaaa ccctctggtt gatttactgt
     8101 ggattcacaa tctgcgagat tatcctttta gtcttgggcg gtatgcctct cttcgatgca
     8161 atttgccaca gttttgccac catggccact ggaggcttcg gtaccaagaa tgcgaacatc
     8221 gcctattaca aaagcggatt catccaatgg gtcattacga ttttcatgtt ccttgcaggt
     8281 atgaatttta cactgcacta tcatttccta aaaggacgaa taggtgctgt ccttagagac
     8341 accgaattta aggtttactt tgcgatcgtt gtcatatcat cactagttat cgcggtggat
     8401 ttattagtat cgggagtatt tagaaatata gaagattcgc tgcgccatgc atccttccag
     8461 gtatcctcga ttctgacgac cactggtttt gcttccacag atttcgggca gtggccacag
     8521 ctctctcaga ccgttctttt cttattgatg tttatcggcg ggtgttctgg ctcgacagga
     8581 ggtggagtca aagtcattcg cctcgtggtg cttttcaaaa tggctgtcaa cgagatgcgc
     8641 tatgtcgcaa atccgcgagg tgtctacact atttttatgg gagacaaggc attgcgaaag
     8701 aatgtcatct atgacatatc cggtctcgtt tttctctact ttgctttttt ctttctctct
     8761 gtgttcgtaa ttagctttag cggtgtagac gtgctgacta atatcacggc ggttatcgct
     8821 aatcttggta acattggccc cgggctagga aaggttgggc cagccttcaa ctacggattt
     8881 ttcccctggt gggcaaaact ttggctttcg ctgaccatgc tagtaggaag gctggaagtc
     8941 tataccgtgc tagcgctctt tacaaggaag ttttggcgcg tgtaggggaa aattagacgg
     9001 gatttagggg aagaattaga cgggatttac gggattgatg aggatttaca ggatgattgg
     9061 acaggattta caggatcgat aaggatttac aggatgattg gacaggaaag caggtcgata
     9121 ttttctccga tcagtaaagc aatttcagcg tcattattcc aaaagcaggg attccgaatt
     9181 cgacaattgc agagccgcca tcttgcttta tggcgattgg ctttccacca ttttcgaaca
     9241 aatcgcaggc aaataccatt ctagggggtg gagacaagcg caattttcta ttgccaccaa
     9301 gtccagttgg atctcgaagc cgtagcatcc atccttcgcc ttcatttggc cgcttccaaa
     9361 caagcacctc gagatctcct aatccagcaa cttctgccgc aagcgaactt atttccgagg
     9421 ccccatgata gggcaggcag gcaggcggct ggaaggcttc tgcggcgagt ctccaggcct
     9481 tagcttcgcg ccagtcaccc ttatgaggcg ctatgcgaaa tcgatatgta agactgcctt
     9541 cgatccattg cggaaagttg gtcccccatt gattgttgaa taggttgaag ttgaggatag
     9601 gcggcccagg aagcgccctt ccatcgaagc gctctatacc gatcgaccca atcgaaaaga
     9661 gcggcgaatc taggggcata aatagaaggc cctctccaga atcttcgaca tcgatccaac
     9721 gctcgcaggc ataaagaatg cgattggcgt ctttttctat gtcatgcgct ggatcgagga
     9781 cccatcctgt cttattgatg gcatagtgcg gatcctgcgc tgcaagggga aacacgatat
     9841 gacccgattc gaggaattgc gtagcgcttt tgtcctcaag gtggtaagtt atgtcgagcc
     9901 atggctgccc tcgcggaaga gtcaattttt ggatgatacg gcgggcattg ccataggtat
     9961 ccatgctctc aggctcctgc gaccaaacaa ccttgagcgc accccaatca acgccatttg
    10021 taatcgaaat attttctagg gagctcaggt aagtatggtg cacaattttg ggatattcag
    10081 gctttccaaa atcttcgaca aaccagggtt ctagatcgta ggcataatcg cgaagataag
    10141 cagtgatctc atcgcggcta aagatatcgt agcggtatga accgaagcct tctctggttt
    10201 cggcatcgac ccattctcgg ccagatctaa gatcgaaaaa gctcgatatg cttccagatt
    10261 cggcatcaac aaccaccctg ataaggccat tatcgagtac gtcttttctt ccttcctttc
    10321 gggctatgag ctgagaagcg gcacgtggct tctcagcctt ctctacgatc tcgatgtttc
    10381 gtagaccgag gggtgcaatt ggtccaatat cggcccagag ctcgccatcc agccttacag
    10441 tcggacatag ctgaccctct acaagtacct gagcgctatc cgacatctgg cctagcctga
    10501 ctagcccatt atgctcccag agttggtggt ttctgacaga gtaaaggcag cgcgattttt
    10561 ccgaacctaa gctataagaa tcactctttt ctagtgattt ttctaacgaa ttgaccgctg
    10621 cgacaaatcg atctgccaaa gcagccttct ctcgccagga tcccctgatc ctaccccagg
    10681 cgccttcctc tagaagtttt gtgaatcgct ctttgtcgta gacacgcccg ccttcggcgg
    10741 gcggattcag cgccagcttt gtgtcgagtc cccacgtgtg ttctccaaag agcgagatcg
    10801 cttcatatga atcgtggatc atagttgaaa agccatctgg cggcctgtct ttggattcga
    10861 gcccttttag cgcctcaaaa ctctctatcg cagcaaggcg ttcgcggtac tgccgtatca
    10921 ttccaagctc ttcaggcatt gtcgcaatac catggatcca gctgtccgca aggtcagagc
    10981 gtaccacagg taactctagc ttgagatctc ggagagcgat ggcaaaatcg tcaaggcttc
    11041 ccacaaccac atcgattcca ggatatgcgg cctcaatctg ggctactgcc tcggtaatcg
    11101 cctctgctgg ctggggccct ccattgtcag aggcgtggat tagagctagc caaatcggaa
    11161 attcgtatcc cggaggtgga agaatcggcg agccataacc ccccttggaa tacatggtag
    11221 ttactcgagt atggtctggt ccttcccaat cgaatagtat tggaacatct ggcggagtcg
    11281 aacatgcatt gcagcctata tataagaacg atacgccaga ggcggccatc agtgttgaaa
    11341 gtatacgcgt atgtcctgga acatctgtca gcttggccgc aatggaccgc cttccaaaac
    11401 gtctagaaag acgatatccg ggcagcattg tacgaataaa gtcctctagg ccaaataact
    11461 cggtatggat ggtgaaagga agagcatgcc aagttatgta tccttcagcc accacccttt
    11521 cgagctgctt gccgagcctg gtaccttcga gccgcataag agcattttcg agcggccatg
    11581 caggcagggt ccaagtaaag cggagattcg gcccaaaatc actgcttttt tcgatagtat
    11641 cgagcgcccg tggaatgagg ttccaagcga ggtctgattg cacatcctcc atcgtaccag
    11701 taaaaccgtt atcgaagtgt gtcgagaaaa cgatgaaaac ttttttaagc tcattactca
    11761 tccttttacc gctcctgcgg tgagaccgcg cacgaagtat cgctggagaa ggaaaaacac
    11821 cacaagaggt atcgacatcg acacaaaagc acccgaggca aggaggttcc actcggaacc
    11881 gtactgacct aagagccctt gcagcgccac cgtaagcgga aactttttag gattctgaag
    11941 taacacaagg gcattcatga gatcgttcca tacccacaca aattggaaga ttccaagaga
    12001 tgcaatagcg ggtaccgaaa gaggaataac aatcttacag aaaatgctga attcggaatg
    12061 gccgtctatc cttgcagctt cgaggagttc cttaggcaac tcagcaaaga aagttcgcag
    12121 aaggaagatt gcaaaaggaa gcccatacgc ggtatgggca agccatatcg atatccagct
    12181 tcccgtcatg tgaaaacggc tctgcattcg aagaacagga acccatgttg tctgaagcgg
    12241 gaggacaagg atcgcaatca ccgagaaaaa gacaaagttc ctgcctctaa aattcatgca
    12301 ggctaaggca taggcggcaa gggctccgag cgcaatgggc agaatagtgg aaggaattgt
    12361 gatgataaag ctgttcaaga agttggtcga gaaattatgt gaaccaagga caatcgaata
    12421 attttcaaag gtaaatcgcg gcaccgcgaa tatacgccac cagccgctgg tggcaatatc
    12481 cgactggggc cttagcgacg taataaagag gccaaaagcg ggtatgaacc agattagagc
    12541 cagagtcaac attattatgg tcctcaacgc gtggccccac gcgcggcggg aagaagcgga
    12601 tcggcgagag gaagctacct ggtgatccga accttgttga taagaggaaa cttccaggct
    12661 cattggtaac tctcctgagc cttgattctt ctaacctgaa aaaccataaa tggaataagt
    12721 agaattaaaa gaatcatggc tatggcactt ccatagccaa ccagattgtt attgaacact
    12781 tcccaataaa aagaaaaacc aatggtgcgg ctagagccca taggcccgcc tccggtcatt
    12841 acgagaacca agtctactag cttaaggctg ttgatgatca tggtaattgc gacgaatacg
    12901 atctgatcct cgagcatcgg tagagatatt cgccagaaac aagtccatga gctagcgccg
    12961 tcaaccttgg ctgcttcaag aatctctcga tcgagggact tgtaggctgc cgaaagaact
    13021 gtcatcgaaa gcccagtcca tatccatatt ccagccccaa ttaaggcaat atttacaata
    13081 tccgtggagc ccaaccaagg aatcggcttt ttgccagggc ttatggagct tataaaagca
    13141 ttgagaaggc caatatgcgg gtctgggctg tataggaatc ggaagataac cgaggctgca
    13201 gtggcagaga ttatcatcgg aaggaagatc agcgacttaa agatcttttc caaccttagt
    13261 ccatcggcta gcactgctat agcaagccct attaccaccg taccgcccgc aaagaacaga
    13321 atccacaaaa tgttgttgat tagcgctcct gagggcggcc acttaaggtt gagaaaaagg
    13381 ctatcgccac caccaaggag ccgcgcgtaa tttgcaaagc ccacaaagcg cttaggagaa
    13441 aaaccttggc caccatagaa actcatcttg agcgtactca gacatggata cacaaggtat
    13501 gcgacaagga gaatcagtgc aggcgccaag aacagccaac cctgaataga atctcgtttg
    13561 actttcatct cccaataccc atctcaatgc ctaaggccta gcggaacaga ccggcgcagc
    13621 caagccgcgc cggcaatgat agcaccagaa attaagcgcc gagaataata gcgccgtaaa
    13681 taaacacgta aatcaatagc attgcgaggc aacaccctcg agataattca aagcttccat
    13741 gtacttatct gggttcttga gtatgcgctg taaggctaca aactccgcat cgccaccaac
    13801 cgcgggcggt gccaggtctg agccatcgaa tacgaagctc gtcgcctgag taagctgcct
    13861 tgcctctttg gcggttaaaa gcggataaac agaaaggtcg acattcttgt ttggagacac
    13921 aacggcgccc ttcttggcag atgcccagat cgtgttggcc tctttccccg caaggaagcg
    13981 cataagcgag acgacttctg gccgattcga gaatactacg gcaaggtcac caccgcctac
    14041 aaccggtttg ccccactttg aatcgatcga ggggaagtcg aaaaaatcgt agtccttacc
    14101 aggcgtcaag ttgggaaaat tttgcgccgc aaaggacgac ataaagccac cttcatagta
    14161 catttcggcc gctggatttg gccttagtac ctcgttgaat ccatcgatga agccggtcga
    14221 aagtgcgcca tcggctcctc cagcgagctt cttgtcggta ggcgttacaa gttctatgaa
    14281 acgctttatc gccgtgacaa cagtaggatc ggtccacttt acctcatgcg tcacatggag
    14341 cttcatatac atgtctgggc cggctgtcct cacatagata ttttcaaacc aatcggtaag
    14401 agtccatcca tcctgagctc caatagctag aggcttcttc cctgcggcga catacttgtc
    14461 cgctatttta agaaggtctt cccacgtatc gggcgtctta actccgaggc tggcgaaaga
    14521 tacaggctta taccagaatg tgctcttcga attggccttg acaataagcc catagaactt
    14581 gccatccacc gtccctagat cgatccatgc cttcccgtag ttggcgtcga gcaaagaagt
    14641 gggaagtatg tcatccttct gaccctgatt gaggggaatg aggacccctt ggcgagcgta
    14701 atttgctact tcgccgggac gtggaagaat cgcaatgtct ggaggattgg agctcgcaac
    14761 gcgcgatttg agaacaacgg tagaatctcg attgccctcg tacgaatatg ggattccagt
    14821 ttctgccgta aatctatcca gcacctcgcg gaagcctgcc tcctcatcac cgccccatgt
    14881 agcgagaatt gtgactggct tagtcgaggc tgtggcactc ttgccctgtg cccatgaacc
    14941 gaaagccact accaacatca ataaaatgac taatgttcgt ttcatgccga acctcctttc
    15001 ctgcatcctg gctcaaaggc ctacgatgag gacagctcta gaatatacca taatatggaa
    15061 cttgtcaaat agatattata tattataata tgatatatca tttaatgatt agctagattc
    15121 gcctcgaata agatccgcgg ggatatagac ttttgcattt ccctgtccct ttcctaaaat
    15181 ctgagcatga actatatcta gagccttttg cccaatatca tattcatgct gcttgaggtg
    15241 agtaaaagct ggtggaagat agcaattcgt agggcaatca aaacatacaa tcgcaatgtc
    15301 ctccggtact tttaagccta ggctccgagc cgcctcgcgt gcaagcacgg ctccttcata
    15361 ctcaaccgaa aagacagcat cgatgtcggg atgcgcgctg aggtgttctc gcatttggga
    15421 aagattagcc ttcgcttgct cacaagagat aattccatag cggaagctat ccacataggt
    15481 catccaaaga tcggtatcta gcggtaggcc atagtcagcc agggcttcga cgaacccctc
    15541 gcgtcgctcc tcgatggccg aagtatcctt tattgggccc gagagaaatg cgatccgctg
    15601 ccgtccaagg tctataagat ggcttacccc gatttttgcc gcctctatgt tgttggtccc
    15661 cacgaaagaa gccgcgaggc ctcgaagccg acggtccacg aaactcatag gcatacgttc
    15721 aagaacgagt cgcagcaccg aaggattgta gttctcgccg tgcacaggca gcacgataat
    15781 accatcgaca ttcaactgcc ttagatgctc tattgctttt tcctccagct caggtcgatc
    15841 taatgtccga cataagatga ggctgtaccc gagttcccgg catcggtctt ctattccaaa
    15901 gagtaggcca agcccgaatg tttccgaaaa atcaggcatt acaaggccaa tattctttcc
    15961 taaaagacca attttcgccc tttttgcggc tatttctggc ctagcgacga aagaaccctt
    16021 tcccggaaag cgctcgatga tcttatttcc agcgagaagt tcgagggcat gcttgctcgt
    16081 tatgcgactc acagaaaaac gagaagacag ctccttttca gatggcactc tgtcgcctgg
    16141 caatagcttg ccgctgctta tatcggcaag aattgaatca taaatccttc ggtacattgg
    16201 ctctgcgcct tgatcgttgt tcatcgtaat aattccttga caattgatat atcattttat
    16261 atcaatagtc aaggcgaaga agtcaatggg atttcattcc aaggagaata tcctcgagag
    16321 taagccttgt gagaaactct ttgtagtata aattcttgcg ctccacaagc ttgatcttct
    16381 gcgacacagg tagatcaatt gcattctcct tgagaatgat atcgagcgat tccatcctcc
    16441 ccacgccttt cagcttttcg ttcagctcct cctcaacatg ccagcagcca tgctcttcgc
    16501 ctagtcgcca ccaggcttga aaatgatagc gtgtagtgtc tgtcaccacc ccatccagat
    16561 cgaagattac tgctctaagc acaggcttga gcgaaatctc cacttattcg ccaggcttta
    16621 gaacaataag ctctttttta tgtctgaaag cgagaggcgc tctggaggat tggctgctag
    16681 gcggcacccg cgcctgggat tcagtttctc gtgtacagcg catctgattg cctgcgagcg
    16741 ccaaatcgga ctccagcgtc tggtcgatag cggatgcttc gttgcttgcc agcatcctat
    16801 cgcggaatcc tgcaaaccca tacaaaaccg aaagccagca gcctgcccat ataaaaattc
    16861 tgtatcacca gctgcctcgc agtatttctc aatcgcataa ataatatctg cgttgatatg
    16921 gtactgcgcg gtgccggcgg ggtaataggc gattgcctct tctccatctt aggaatgtta
    16981 ttcttattct atatttgcag atatacttca agcctatctg ccgctgcata gaattcaaat
    17041 ggcgcatgag ctatcatgcc gatctttcga tacccaagcg aaacaaagta ttcgatcata
    17101 tcttttgcag ccgcgacatt atcgatgtct acctgaagaa cagagagccc ctctatagag
    17161 cctatcacta caaaggtaac ttttgcgtcc ttaagctcct ttatgctagg gccatcggta
    17221 tgcgtattca tgagaataag atcatatatc tgagccgatt gaattaagga tcgcggaata
    17281 ggaataaaaa agcctggcga cgacctactc ttccaccgaa tggcagtacc atcggcg
//
LOCUS       DAOSOB010000042        16966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3400_length_16966_cov_71.971380, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000042 DAOSOB010000000
VERSION     DAOSOB010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 16966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16966
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3400_length_16966_cov_71.971380"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLS88_05720"
     CDS             complement(<1..187)
                     /locus_tag="PLS88_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06872.1"
                     /translation="MNDIIIIIGQWIDNLPSGIGAILILLVGWAIAKLLKLLVPIVLA
                     WLKFDVLSEKIGIKEFLR"
     gene            complement(215..1357)
                     /locus_tag="PLS88_05725"
     CDS             complement(215..1357)
                     /locus_tag="PLS88_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOU06873.1"
                     /translation="MKNIDWEGIVEHLDLVAAKGKKMVWCVVGAGNGGLAMAGHLGIM
                     GFPVHIYNRTNEHLNAIHWYGGIDIEGAVTGFGPVLLASADIGEAVEGSDVIMIVTPS
                     TAHYSLATLMAPFLKDGQIVVLNPGRTGGALEFRAELERQKCKARVVLVEAQTFIYAS
                     RMITRNRAHIFSIKNGVPVSALPSFLTPAVLKVIDVAFPQFIAGSNVLATSLENIGAV
                     FHPALTLLNAGWIESTGGDFEYYLQGISPSVARVLQTIDDERLALARALGIRTVSARE
                     WLYLTYDSVGDDLYTAIRSTVAYAGLKAPRSIDHRYIWEDVPMSLVPMASMAAMYSIE
                     TPAINLIIDLANLMLGQDYKANGRTVHSLGIEGMSVEELHRLVTEG"
     gene            complement(1344..3302)
                     /locus_tag="PLS88_05730"
     CDS             complement(1344..3302)
                     /locus_tag="PLS88_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06874.1"
                     /translation="MYRVKQTDVVGLVKPSIDAHTLGILSFAQMLRDCGIRVEIAGED
                     VNKEIDTVSNDRSLDVLVRWIRDRHIDILGYSYRLDPERGVEIFARLVERLRRLGLMA
                     REGGPIVTLWFAGLPPACRRAAERVPFVDTTFQGNESYEEVLEKLGLPAHLVPEQASV
                     ELAYDKARLELGSEIIKNRKYLELKPIDRSGSKRFGLWGERVVDRVHHGIKHGFPPLM
                     RAHVGPYAEDCEQAVALFLDWTKQLAKGSMLDILSIGSSQLTQSHFGEDWTGLPNGGG
                     VPINSAAEYRQVWEAGRPMLIRTYAGTKHVPDMAKMHEEALDICWHALSFWWFSQLDG
                     RGENTVMQNLEEHFKALRHIASSGKPMEPNVPHQFAFRGCDDVGYIVSGYVAAKAAKM
                     QGIHTLILQIMLNTPKYTWGIQDIAKARALRVLVNELEDPDFAVILQPRGGLDYFSSN
                     LERARAQLASVTMLMDDIEPDNPASPQIIHVVSYSEGVRLADPEVVEESVRITRYALT
                     EYRRLKKKGDMPEYGKSEMVEARTQNLLKEARRMIKFIEATIPNAYSPIGFYSMLKSG
                     VFPLPWLIACKDEFEAAVNQPVQFLHGGVHVVDREGKPLSVSQRLFGISKNLEERGFI
                     PSISHNADSSRQVSGSGGGQKEHLGEEH"
     gene            complement(3408..4454)
                     /locus_tag="PLS88_05735"
     CDS             complement(3408..4454)
                     /locus_tag="PLS88_05735"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06875.1"
                     /translation="MRLLIIMKKSSDQSLAAERNARYLRMVEIYKNVGFEAFLSYAEN
                     IDDIRLSLNNYKPDLVFCGIDHLYENENNISHNLHAWFEEHNVNYIGSDSIAIDLALS
                     KGSLKNRWQREGIRTPAFGLVREEQNKCSEDIKRLLSLNAFPYILKPENLGNSRGIDE
                     NSIVWNEAELRETLDKMRLQFEGIIIVENYLGVSIDFMEITCAMIEGVSGMEFMPAQV
                     SLLEPKRFHIVTTRDKDKKGTIVNPLEADMAASFLPIAKRAFAVAGVRDYARGDFIYA
                     NGEFWAIEINGQPMIPDQWFGGATQFAGLSEDLYLVGIVAAGYRRLKAEGQIAKVFPK
                     QAQALLRETKFEVW"
     gene            4640..5290
                     /locus_tag="PLS88_05740"
     CDS             4640..5290
                     /locus_tag="PLS88_05740"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOU06876.1"
                     /translation="MIANNERQEERDFMVRFQIAARGIRDKRVLDAMRRIPRHLFVPS
                     SYRVEAYEDYPLPIGSGQTISQPYIVAFMAEALMLKGDEIVLEIGTGSGYQTAVLSLL
                     AKKVYSIERIAALLDNARTILESLEISNVELRLADGFYGWEEKAPFDAIILSAAPFDI
                     PSVLLQQLADGGRLVGPVGPLGAQTLVRMTRRGQRFHSEALLDVAFVPMRSGLSEV"
     gene            5438..6175
                     /locus_tag="PLS88_05745"
     CDS             5438..6175
                     /locus_tag="PLS88_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOU06877.1"
                     /translation="MFAQNLTFPHFEEQIKKIDTVVIPTGSFEAHGRHCPLGTDIIIP
                     ERLCKDLDSAMGDQVLIAPSINYGYTPLLNAFSGTIHLPAELLISIYSEVAKGFVSWG
                     AKNIVFMNGHGGNIPMLTVACDRIAKAGASAMVISWWATFSKEILGICNSQGHAGEDE
                     TSVIMAIDATLVDRQSRSTHMKKAFSLPLATPDQVAERYPNAMNGDSLLATKEKGERL
                     LAMMLEKNIEYINRLRRKDLLVPIEVG"
     gene            complement(6282..7277)
                     /locus_tag="PLS88_05750"
     CDS             complement(6282..7277)
                     /locus_tag="PLS88_05750"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOU06878.1"
                     /translation="MIPIIDMHADIIMDIVRKEGISDENSVLKRFKQHIDRMKSGGIQ
                     GAVLVDCRMAGESAGTRNFEEFIAVTKLLRSCKDSSYIIATSSQELDNSIRDERWVGI
                     LCYEGLRAANGDLSWLRRLYKEAGLRIAMLTHNDSNAFGTGAFDPNGSPRAENPLGLT
                     EAGREAVKLMNEMGILIDLAHASPMTRLDILSCSKKPVMLSHTSSAEVYNNGRNLSDK
                     DMHDIAEQGGLIGCMTSPAALADIKDRANHTLERYMHHLRHMIDVAGEDHVGLGLHFC
                     EYLYTQEEYPPVKGLEDASRAYVIIEELGKCGLSSYQIEKIAYRNFARVFKEATD"
     gene            complement(7314..8288)
                     /locus_tag="PLS88_05755"
     CDS             complement(7314..8288)
                     /locus_tag="PLS88_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU06879.1"
                     /translation="MSNNQSELPILQVRNLKKYFHVAKGTLHAVDDISFDIKKGGTLG
                     MVGESGCGKSTTGRLLVRLHESDGGSINYNGIEITKASHKDMKMLRTKMQMIFQDPYS
                     SINPHHTVSSIIGEPLKIYKKVRNMQEYEEKVRSLMETVGLADRLYDAYPHELDGGRR
                     QRIGIARALALEPQFIVCDEPVSALDVSIQAQILNLLMDLQESLNLTYLFITHDLSVV
                     KHISDDIVVMYLGQIVEKANTEELFKHPIHPYSKALESAIPIPRLNARKERIILKSEV
                     VSPIDPKPGCRFAPRCNYAIASCGESDPILREVCPDRFVACHRAEMFV"
     gene            complement(8269..9252)
                     /locus_tag="PLS88_05760"
     CDS             complement(8269..9252)
                     /locus_tag="PLS88_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06880.1"
                     /translation="MDKILEIKELHVHYYSNRRTVRAVNNLSLDMQRGRVLGLVGETG
                     AGKTTLALSILNMISAPQGKIISGSIRFDNADILGMSNQELREMRGKDIAMIFQDPMT
                     ALNPVLTIGEQIAETIFKHEKVSKAEAWSKAEEMLETVGIPGVRAREYPHQFSGGMKQ
                     RVVIALSLACNPRLLLADEPTTALDVTIQAQVLDLMRGLIKTNNTSVLFITHSLGLVV
                     ENCDEVATMYAGEVVEYGTVKQVFGNPLHPYTKGLLGSLPDYSEGSPRLRPIPGLMPD
                     PTNLPKGCYFSPRCPNVMPKCHELHPENSSIEGHLVKCHQYQENLHVEQSI"
     gene            complement(9263..10183)
                     /locus_tag="PLS88_05765"
     CDS             complement(9263..10183)
                     /locus_tag="PLS88_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06881.1"
                     /translation="MSNQTFLRFFKRQSKNISQFWRQFSRNKLAVASLILVLIFALIA
                     IFADVLYSYDFVTSNSLENALLKPGETGTVYFQEKDIEHTFILGSDNYGRDILGRLVH
                     GARISMIIGFVTVAMAVVIGGILGAIAGYKGGLFDTIIMRLTDIILSIPTILMSIAIV
                     AALGNSFFNLLIAISTSLIPGYIRIVRASIISIKDQEYVEAARVIGAGDFRIVLKHIL
                     PNITSPIIVQSTLNVGSSILSASWLSFLGLGILPPKPEWGNMLSEAREFIQYAPHLLF
                     APGIAILLTVLAYNIIGDGLRDALDPKLRR"
     gene            complement(10180..11169)
                     /locus_tag="PLS88_05770"
     CDS             complement(10180..11169)
                     /locus_tag="PLS88_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06882.1"
                     /translation="MFRYIMRRILMLIPVLLGVTFIVFSLMYITPSDPAQMMLGANAS
                     PAAYKQMREDMGLDAPFIVQYGRFLFGYKHPVFGYKGLLFGDLGRSYVSNRIIFELIL
                     SSFPNTLILSVGALILALLLGIPIGIISATKPYSVADMVFTVFALLGASMPVFWIAMV
                     LIYTFANNLRIFPAIANPRDFLSLVLPIITLSMNSMAIIMRMTRSSMLDVMQQDYIRT
                     ARVKGMDEKTVVFKHALRNALIPVVTVAGLQFGSLLGGAVLTEKIFSYPGLGSIMVDA
                     IFQKDTPQILGSVCFVAITFTVINLAVDILYGFIDPRIKSTFSTKKKLVRGNQ"
     gene            complement(11274..13022)
                     /locus_tag="PLS88_05775"
     CDS             complement(11274..13022)
                     /locus_tag="PLS88_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU06883.1"
                     /translation="MKKFLSIVLICLALGGVVFAQAKKGPTLRNRDPDTLVFVLAAAP
                     QPLDPNIYTTLNEAQIMREINETLIGMKSDNTFYPILAETIPTIQNGLVSKDGSVYTI
                     KLRKGVKFHNGNPFTADDVVFSFNRILHHPKSSSKSMFSAVLSVVKVDDYTVKITFGK
                     LKDPEYATVALTSWEDRAKYMTASPYGPALNILAHYCSGIEDKETVEAAGADYGTKIV
                     VGTGPYKFVSWPNPQEVQLTRNEAWWGDANTVAFKNIVFRAMSEQPQVNNVLITGEVD
                     MAFNLSKLDIGNLERNGLVITTKPGVTIWYGVFNMNSDLVGQKKNGKLDLSGQYTEGD
                     STHLRRAIFYSINPVPFIKQVDIFNGYAVPANQMMQPGFLGYVEGAPAGTPFAEFNEK
                     TWYYNKEKAKAEFAMLSPEFRAKLGPESVTLVVGNVTDRVKIANNIKDQVKTTLGFDL
                     IKVVPMVNAQIIQQSKTGDGYDMVVTAWFTPTMDPDYTCIIYGGDSIGTGMNGALYNN
                     DIVNKNIQIGRYSLDRKTREEAYIKVQTQLMTDKPYFPMVYENVLFASHPRVGNLDQA
                     IFKTKLVDIHLLTRLQ"
     gene            complement(13136..13978)
                     /locus_tag="PLS88_05780"
     CDS             complement(13136..13978)
                     /locus_tag="PLS88_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOU06884.1"
                     /translation="MKVFISVDMEGIHGAVSWSDMEGNGHQTVYENAWKELSWIVEGI
                     NRSKYNDEIREICICDSHARGEGIPFRSFGDSRINLIRGYPRPFYMLEGLDESFDLLM
                     LVGYHAKIGALHGLMDHSYSSSCIYCVRLNGQEMSEADINAMLASHYGVPVCFVSGDD
                     ILEKEIEEHFPIPPIFICTKQGLGRFAAKMYFEDNLKPKFVEGAMQAIDRKDTFKPLT
                     LAPAYDLEIDFASTVIADAVSVIPGLERRGGRRVLYRSTDMRSIYRMIHAAAMLGGKF
                     AAFT"
     gene            complement(14043..15218)
                     /gene="iadA"
                     /locus_tag="PLS88_05785"
     CDS             complement(14043..15218)
                     /gene="iadA"
                     /locus_tag="PLS88_05785"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HOU06885.1"
                     /translation="MSMLLLKNGEVYAPYPLGFRDILIGGDRIISVDSNIDVSSSISH
                     VETIDASGCIVVPGLIDGHQHFIGGGGEGGFETRTPELQISMNFLNGVTTAIGLLGTD
                     SLTRSLEDLYAKTKAFNTEGMTAFMLTGAYWYPSPSITGSVAKDILFCDPVIGVKLAL
                     SDTRGPYMDVADLARLASDVFVAALIANKPGIITVHTGIRPQALDLIMEVIKNPGIRP
                     AMFVPTHINRKNPKLLDQVFELAKGGGMVDATCRKEVNANSENMSAADFAMLAREKGL
                     LDHVTFSSDAGGSLPEWNGDHSRITGMGIGTPETLMFELRNLVQQKHLPLEEALLPLT
                     SNPARNYGLEGRKGTIREEADADILVLESDGLEIRDVISRGRLVVRRGEVIKKGYFE"
     gene            complement(15248..16108)
                     /locus_tag="PLS88_05790"
     CDS             complement(15248..16108)
                     /locus_tag="PLS88_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOU06886.1"
                     /translation="MILDKIRKMLPEMPNSYQKIGSYMLDHEQSVAFSSIHTISRSIG
                     VSSATLVRFAKSLGFEGYQEFKHEIQDEIRHRLNPYDKIALSELDILPDEKRLQKLFQ
                     NEYNNTRSTFSNIKMADLEIMGEGIKKARRVYLSAFGATAYVAQIFKYTLMSSLEKEV
                     YVVSGSVSDYAPVLKSFSSEDVMFLMTFPPYSLEVRHVAKVVKEKSGSLFLFTDSAAC
                     PVYSEADKVIRCITNSLLLTNSFAGLISILHVLVQMVYLDEKESAAASRQKTISMQES
                     GYLVISNPKE"
     gene            16278..>16966
                     /locus_tag="PLS88_05795"
     CDS             16278..>16966
                     /locus_tag="PLS88_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06887.1"
                     /translation="MKRLLLLFAILAVVAVSPALAETEAELKAAYENAVKLATAAPQD
                     YTLNWQAARAARSYGDYLVVHQTTGWKETARAAAKEGMKYGEIAFNLNPSGIEGWYYY
                     GLCVGTYSDCVSIVTALTEGLKGKTQMGFENAYKFDKTYDNGGPILALGRFWQVLPGI
                     AGQDRKKAEKLFDEYIALFGSSPKANSDAWYFRGQLYKDTKRPDLAKADLEKAASMGN
                     ENAKKLLAEMK"
BASE COUNT         4621 a         4113 c         3509 g         4723 t
ORIGIN      
        1 tgcgcaaaaa ttccttgata ccgatcttct ccgaaagcac atcgaacttg agccaggcta
       61 aaacaattgg cacaaggagc ttaaggagct tggcaattgc ccagcctacc aatagaataa
      121 ggattgcccc aatccccgat ggtaaattgt cgatccattg tcctattata attataatat
      181 cgttcattca attttcctcc attgagagcc ctgtttagcc ctcggtgaca agacggtgga
      241 gttcctccac ggacatacct tcgatgccga gagaatgtac cgtgcgtcca ttggctttat
      301 agtcctgccc aagcattaga ttcgcaagat ctatgattaa gtttattgct ggagtctcaa
      361 tgctatacat agcagccata cttgccatag gtacaaggct cataggtaca tcttcccata
      421 tatagcgatg atcgatagaa cgcggcgcct ttagcccggc ataagctacg gttgagcgaa
      481 tcgcggtata tagatcatct cccactgaat cgtaagttag atagagccat tctctcgccg
      541 agaccgtccg gattcctaga gctcgggcta atgcaagcct ttcatcgtct attgtttgga
      601 gcacccttgc cacagatggt gaaatgcctt gaagataata ctcgaaatct ccgcctgtgg
      661 actctatcca tcctgcattt agaagcgtca gagcggggtg aaacacagcc ccgatattct
      721 cgaggctcgt agctaaaaca ttgctccctg caatgaactg aggaaaagca acatcgatca
      781 cttttagcac agccggggta agaaacgaag gtaatgcgct aacgggcact ccatttttga
      841 tactaaaaat atgagctcta tttctggtga tcatccgtga ggcatatatg aacgtttgcg
      901 cttcgactaa aactacacga gccttgcatt tctggcgctc gagctcggcc ctgaattcta
      961 atgcccctcc agttcttccg ggattgagca caacaatctg tccatccttt agaaaaggcg
     1021 ccatcaatgt agctagacta tagtgcgcag tcgagggagt aactatcata ataacatccg
     1081 aaccttctac agcttcccca atatctgcgc ttgctaggag cactggtcca aagccagtca
     1141 ctgctccttc aatatcgatg cccccatacc agtgaatcgc atttagatgc tcattggtgc
     1201 gattgtagat gtgaaccgga aagcccataa ttccgagatg gcctgccata gcaagtccgc
     1261 cattccccgc ccccactaca caccaaacca tctttttgcc tttagcagct acaagatcga
     1321 ggtgttctac tatcccctcc caatcaatgt tcttcaccta agtgctcctt ttgcccacct
     1381 ccgctcccac ttacctgccg gcttgagtca gcattatgcg aaatcgaagg tatgaagccc
     1441 ctctcttcga gatttttaga tatcccaaag agccgctgac tcacggagag tggcttgcct
     1501 tccctatcga ccacatgaac gcctccatgc agaaactgca cgggctgatt aaccgccgct
     1561 tcgaattcat ctttacaagc aattagccaa ggaagaggaa aaacgccaga ttttagcatt
     1621 gaataaaaac ctattggact ataggcattc ggaatagtgg cttcaatgaa tttgatcatc
     1681 ctcctagcct ctttcaataa gttttgtgtt cgagcctcga ccatttccga cttgccatat
     1741 tctggcatat cgcctttctt ctttagacgg cgatattccg tgagcgcgta ccttgtgatt
     1801 cgcaccgatt cttccaccac ttcaggatcc gcaagcctta cgccttctga ataactcacc
     1861 acatgaataa tctgaggact tgcgggattg tccggctcga tatcatccat gagcatggta
     1921 acagaagcca gctgagcgcg tgccctctct aaattcgatg agaagtagtc gagcccacct
     1981 cgaggctgca aaattaccgc aaaatctgga tcttccaatt cattaacaag caccctcaag
     2041 gcgcgcgctt tggcaatatc ctggattccc catgtatatt tgggcgtatt gagcataatc
     2101 tgaagaatca aagtgtgaat cccttgcatc tttgctgcct tagctgcaac ataaccagat
     2161 acaatgtaac caacatcatc gcatccacga aaagcaaact gatgtggcac atttggctcc
     2221 atgggttttc cgctcgaggc gatatgacgc agtgccttaa aatgctcttc caggttctgc
     2281 atgactgtat tttctccacg tccatcgagc tgagagaacc accaaaaaga aagagcgtgc
     2341 cagcagatat ccagagcttc ttcatgcatt tttgccatat ctggcacatg ttttgtgcct
     2401 gcataggtac ggataagcat aggccgtcca gcttcccaga cttgtcgata ttccgccgcg
     2461 ctatttatgg gtacgccacc accgttcgga aggcctgtcc aatcttcgcc aaaatggctc
     2521 tgtgtcaact gagaacttcc aatagagaga atatccagca tactcccttt cgccagttgc
     2581 tttgtccagt cgagaaaaag tgcgactgct tgctcacaat cctctgcata agggcctaca
     2641 tgggctcgca tgagcggagg gaatccatgc ttaatgccat gatgtacgcg atccactact
     2701 cgctctcccc aaaggccgaa tcttttggaa ccagaacgat ctataggttt taattctaaa
     2761 tactttctgt ttttgataat ttcactgccg agctcgaggc gcgccttgtc gtaagcgagc
     2821 tccacgctcg cttgctcggg cacaaggtgt gccggcagcc ccaacttttc cagtacctct
     2881 tcataggact cgttaccctg gaacgtagta tccacaaagg gcacacgctc tgccgcccgc
     2941 ctacaggcgg gcggcagacc agcaaaccac agagtaacga ttgggcctcc ttccctcgcc
     3001 attagcccga ggcgccgaag gcgctcgaca aggcgcgcaa aaatctcgac cccgcgttca
     3061 ggatcgagcc gatatgaata ccctagaata tctatatgtc tatcgcgtat ccatctaact
     3121 aaaacatcca acgatctatc attagatact gtgtcaatct ctttgttgac atcttcgcca
     3181 gcaatctcca ctcttatgcc gcaatctcgc aacatctgtg caaacgacag aatgccaagc
     3241 gtatgtgcat ctatcgaagg ctttacaagg ccaactacat cagtctgctt tactctgtac
     3301 aacttaaaat ctccttttag cagcttgtcc tggattgctc aaaccgtcat ttcacttcag
     3361 ttctctaaca tcttagaacc tctttttata tacttaaaag gattacctta ccaaacctcg
     3421 aattttgtct ctctaagcag agcttgcgcc tgctttggaa aaactttcgc aatctgccct
     3481 tcagctttta gtcgtcgata accagctgcg acaattccca ctaggtacag atcctccgat
     3541 aacccagcaa attgagttgc tccaccaaac cattggtcgg gtatcatcgg ttgaccgttg
     3601 atttctatcg cccaaaattc tccattagca tagataaaat ccccacgtgc gtagtctcgt
     3661 actccggcaa cggcaaaagc tctcttcgca ataggcagaa atgaagcggc catatcagct
     3721 tcgagcggat ttacgatggt tcctttcttg tctttatcgc gggttgttac gatatgaaat
     3781 cgtttgggct ctaagagaga gacttgtgca ggcatgaatt ccatgcctga aaccccttcg
     3841 atcatagcgc aggtaatctc cataaaatca atagaaacgc caagataatt ctctactatt
     3901 atgattcctt caaattgaag gcgcatttta tcaagagtct cgcgaagctc agcctcattc
     3961 caaacgatgc tgttttcatc tatacctctc gaattgccaa ggttttctgg cttgagaatg
     4021 tagggaaagg cgtttaatga taaaagcctt ttgatatcct cactgcattt attctgttct
     4081 tctcgaacaa gaccgaaggc gggcgtcctt attccctctc tttgccatct attttttagg
     4141 gagcccttgg aaagagctag gtcaattgct atagaatccg agccaatata attgacatta
     4201 tgctcttcga accatgcatg taagttatgc gatatattat tctcattttc atataaatga
     4261 tctattccac aaaacacaag atctggcttg taattattaa ggctaagtct aatatcgtca
     4321 atgttctctg cataagataa aaaagcctca aaaccaacat tcttatagat ttcaaccatt
     4381 cttaggtatc ttgcattgcg ctctgcggca agactttgat cggagctttt tttcatgatt
     4441 attaacaatc tcatctgacc ctctgtttaa attctaaatt tgttcaaatt ctgcatataa
     4501 atatgtaaca caatttgagg aatttagcta atttgtgctt tgtctgtatc gaaaatcctc
     4561 cagacttctg gaatattcga ccatttcaaa acacataaag gcaaatatcg aatttgctct
     4621 tatgctataa taataaaaga tgatagctaa taatgaacga caggaagagc gcgattttat
     4681 ggttcgcttc caaattgcag cacgcggtat aagggataag cgcgttcttg atgcgatgcg
     4741 tcgtatccct cgtcatctat ttgtgccatc gtcttatcga gtcgaagcct acgaagatta
     4801 ccctttgcca ataggctcgg gtcagaccat ttctcagcca tatattgtgg cctttatggc
     4861 cgaggcgctg atgctcaagg gagatgagat agttctagaa ataggtacgg gctcgggcta
     4921 tcagaccgcg gtgctctcct tacttgctaa aaaagtatat agtatcgagc ggatagccgc
     4981 tctcttagat aatgcacgaa ctatcctcga atcccttgag atatcaaatg tcgagcttcg
     5041 tctggcagat ggtttctatg gatgggaaga gaaagctccc tttgatgcta taatattgag
     5101 cgcggcgccc ttcgatattc cgagcgttct attgcagcag ttggcagatg gcggcaggct
     5161 cgtggggccc gttgggcctc tcggagcaca gactctcgta aggatgacga ggcgtggcca
     5221 gcgcttccat agcgaggctc tcttggatgt tgcctttgtg ccaatgcgat ccgggttgag
     5281 cgaggtttaa cgcaagaata cctcttcaaa aacgctcctc aaatactgta attttatatt
     5341 gaatctgata tgctggtttc gattatgtaa tcatagagcc aaggttgaaa aacaatggga
     5401 ataattattc gcttaacatc atatattgga gagaattatg tttgcacaaa atttgacatt
     5461 tccccatttc gaagagcaga taaagaagat cgacacagtt gtcatcccaa ccggtagctt
     5521 tgaggctcat ggcaggcact gtcctctagg aacagatatc attatcccgg agcgtctttg
     5581 caaagatctg gattctgcca tgggagatca agtccttatc gctccttcta taaattatgg
     5641 ttacacgccg ctcttgaatg ctttttctgg taccattcat cttcctgcag agcttctcat
     5701 atctatctat tccgaagtag caaaaggttt tgtaagctgg ggcgctaaga atattgtttt
     5761 tatgaatggt catggtggca atattcctat gttgaccgtt gcttgtgacc ggatagccaa
     5821 agcaggagcc tcggcgatgg taatcagttg gtgggcaaca ttctctaagg agatactcgg
     5881 catttgcaat tctcaaggcc acgcaggaga agacgagact tcggttatta tggcaatcga
     5941 cgccactttg gtcgacaggc aatctagaag tacacatatg aaaaaggcat tctctcttcc
     6001 actcgctaca cctgatcagg tcgcggagcg ctatccaaat gccatgaatg gagattcact
     6061 tctcgcaaca aaggaaaagg gagagcgcct tcttgctatg atgctcgaaa agaacatcga
     6121 atacattaat cgcttgcgca gaaaagatct gctagtgcct atcgaggtcg gctaagatct
     6181 atatttatcg ctagaccccg ccaaaggcgc agacttccat ttgctttcgt ttcgctaata
     6241 tatctcgtta ttttttcatt atatgccgag aaaagactga ctcagtcagt cgcttctttg
     6301 aaaaccctag caaaattgcg atatgcgatt ttttcgattt gatatgaaga aaggccgcat
     6361 ttccctagtt cttcgatgat tacgtaagcc cttgatgcgt cttctaaccc tttaactgga
     6421 gggtattctt cttgcgtata taggtattca cagaaatgca gaccaagccc cacatgatct
     6481 tcgccagcta catcgatcat atgccgcaga tgatgcatat atcgctcgag cgtatgattg
     6541 gctctatctt ttatgtcagc caatgcggct gggcttgtca tgcagcctat caaaccaccc
     6601 tgctccgcta tgtcatgcat atctttatca gagaggtttc tgccattgtt atatacttcg
     6661 gcgctcgatg tatgagacag catgacaggc ttcttagaac acgacaggat atctagtctg
     6721 gtcatgggac tagcatgcgc caaatctatt agaattccca tctcattcat aagcttcact
     6781 gcttcccgtc ccgcttctgt caggccaagg ggattctcag ctctagggct tccatttgga
     6841 tcgaaagctc cagttccaaa tgcattgcta tcgttatgag tgagcatcgc aattcgaaga
     6901 ccggcttcct tgtataatct tctcagccat gaaagatcgc cattcgctgc acgcaacccc
     6961 tcatagcata atattccaac ccatcgctca tctctaattg aattatcgag ctcttgagaa
     7021 gatgtcgcta tgatatacga gctatcctta catgaccgca atagcttggt taccgcaata
     7081 aattcttcga agttccttgt ccctgcggat tctccagcca tgcgacaatc gaccaaaaca
     7141 gctccctgga tacctccact tttcattcta tctatatgct gcttgaatct cttgagcact
     7201 gaattctcat cagagatgcc ttcctttcga acgatatcca tgatgatgtc ggcatgcatg
     7261 tcaataatag gtatcataga tttcgccagc tctcctatgg ccagaaatag ccgtcagaca
     7321 aacatctctg cgcgatggca ggctacaaag cgatcgggac agacttctct gagaattgga
     7381 tcagattcgc cacaagaggc tatggcataa ttacagcgag gcgcaaagcg gcaacctggc
     7441 tttggatcga ttggcgacac aacctcgctt tttagtatta ttcgttcctt tctggcattg
     7501 agccttggaa taggaatagc cgattccaag gccttactat agggatgaat tggatgctta
     7561 aagagctctt ccgtatttgc tttctcgact atctgcccca ggtacattac aacgatatca
     7621 tcagatatat gctttaccac tgaaagatca tgggtaataa aaagatatgt taggttcaaa
     7681 gactcttgaa gatccattag caagttgagt atctgggctt gaatcgatac atccagtgca
     7741 gataccggtt catcgcagac tatgaattga ggttcaaggg caagagcacg cgcaatacct
     7801 atgcgctgtc ttcgacctcc atccagttca tgaggatagg cgtcatataa tctatcagct
     7861 aaaccgacag tttccatgag cgagcgcact ttttcctcat attcctgcat attcctgacc
     7921 tttttataga tcttgagtgg ctcgccgatg atcgatgaga cagtatggtg aggattaata
     7981 gaagaatatg gatcctggaa gatcatctgc attttagtgc gcagcatctt catgtcctta
     8041 tgcgaggctt ttgtaatctc aattccatta taattgatag agcctccatc tgattcatga
     8101 aggcggacaa gtagtcttcc agtagtagat ttgccacagc cagattcgcc gaccatgcca
     8161 agcgttcccc ccttttttat atcgaaggaa atgtcatcca cagcatggag agtccctttt
     8221 gcgacatgga agtatttttt taggtttctt acttgtagaa taggcaattc agattgattg
     8281 ttcgacatgc aaattctcct gatactgatg gcatttcacc aaatggcctt ctatagaact
     8341 gttttcggga tgcaattcat gacacttagg cattacatta gggcatcgtg ggctgaaata
     8401 gcatcccttt ggcaaattgg ttggatcggg cataagccca ggtataggcc gaaggcgggg
     8461 agagccttca ctataatcag ggagagagcc caaaagaccc tttgtataag gatgaagtgg
     8521 attcccaaaa acctgtttaa ccgtaccata ctctacaacc tcgcctgcat acatggttgc
     8581 tacctcatcg cagttctcga ctacaagccc taatgaatgg gtaataaata gaaccgatgt
     8641 attattggtt tttatgaggc cgcgcatcaa atcgagcacc tgtgcctgaa ttgtgacatc
     8701 taacgccgtt gtcggttcat ctgccaaaag aagcctagga ttgcatgcga gcgaaagcgc
     8761 aatgacaaca cgttgtttca ttccccctga aaactggtgg ggatattccc ttgctctgac
     8821 accaggtata ccgacagtct cgagcatttc ctcagcctta gaccaggcct cagccttgga
     8881 aaccttctcg tgcttaaaaa ttgtctccgc tatctgctct cctattgtca gcactggatt
     8941 gagcgctgtc atagggtcct gaaaaatcat cgctatatct tttccccgca tctctcgcag
     9001 ctcctgattg gacataccga gaatatctgc attgtcgaag cggatagatc cagagattat
     9061 cttgccctgg ggcgcgctga tcatattgag tatggaaaga gctagcgttg ttttaccagc
     9121 tcctgtttca ccaacaagac ccaatactcg gcctctctgc atatccagcg aaagattatt
     9181 aaccgccctg accgttcttc gatttgaata ataatgtaca tgcaactctt ttatttccag
     9241 aatcttatcc ataattcgtt tcctaacgcc tgagttttgg atctagagca tcgcgcagtc
     9301 catcgccaat gatattgtag gccaaaacag tgagcaaaat cgctattcct ggcgcaaata
     9361 gaagatgcgg tgcgtattgg atgaattctc tagcctcgga gagcatattg ccccattccg
     9421 gctttggcgg aagaattcct agccccaaaa aagacaacca agaggccgaa agtatcgatg
     9481 agcctacgtt gagcgtagac tgaacaataa tcggagaagt gatatttggc agtatgtgct
     9541 tgagcacaat gcgaaaatct ccagcaccta ttacgcgagc tgcttctaca tattcctgat
     9601 ctttgatcga gatgatagaa gcgcgaacta ttcgtatata gccaggaatc aaggatgtgc
     9661 ttatagcgat tagcaaattg aaaaaactat tcccaagtgc ggccacaatg gctatcgaca
     9721 tcagaatggt cggaatagaa aggattatat cggtaagtcg cataatgatt gtatcgaaca
     9781 agcctccctt atatccggct atagctccta agatgccgcc tattacaact gccattgcta
     9841 cagtcacaaa accgatgatc atagatattc ttgcaccatg gactagcctg ccaagaatat
     9901 cacgaccata gttgtccgaa cccaggataa aagtgtgctc tatatccttt tcttgaaaat
     9961 agaccgtacc ggtttctccg ggctttagaa gcgcattctc gagcgaatta gacgtaacga
    10021 aatcatacga atagagaaca tcagcaaaaa tggcaataag tgcgaatatc agaacgagaa
    10081 tcaagcttgc aactgcgagt ttgttcctag agaactgcct ccagaattga gagatatttt
    10141 ttgattgcct cttaaagaac ctcaaaaatg tctggttact cattggttgc ctcgcactag
    10201 tttcttcttt gtcgaaaaag tggattttat gcgtggatcg ataaatccat agagaatgtc
    10261 gaccgcaaga tttatgacgg tgaatgttat tgcaacgaaa cagaccgatc cgagaatctg
    10321 tggtgtatcc ttttgaaata tcgcatccac cattatggag ccaagtcctg gatatgaaaa
    10381 aatcttttcg gttagaacag ctccaccaag caagcttcca aactgaagac ccgcgacggt
    10441 aactactggg ataagggcat tgcgcaaggc atgcttaaag accactgtct tttcgtccat
    10501 acctttaact ctcgctgtcc ttatataatc ttgctgcatg acatcgagca tggatgaacg
    10561 tgtcattcgc atgataatag ccatcgaatt catactcaaa gtgataattg gtagcactag
    10621 agatagaaaa tcgcgcggat tggctattgc aggaaaaatg cgtaggttgt ttgcaaaagt
    10681 atagatgagc accattgcaa tccagaatac cggcattgat gctcccaaga gagcaaaaac
    10741 cgtaaaaacc atatcggcca cagaatatgg ctttgttgca gagatgattc caattggaat
    10801 acccagtagc agtgcaagaa taagcgcccc tactgaaagg attagtgtgt tagggaaaga
    10861 actgagaatg agttcgaaaa ttatcctatt cgacacataa gacctgccaa ggtctccaaa
    10921 caataatcct ttataaccaa aaacagggtg cttatatcca aagagaaatc ttccatactg
    10981 cacaatgaag ggcgcatcca accccatgtc ctctctcatc tgcttatatg ctgcaggtga
    11041 agcatttgct cccaacatca tttgagcggg atctgaggga gtaatgtaca taagagaaaa
    11101 gacgatgaat gtcacgccta acagaactgg aataagcatc agaattcgac gcattatata
    11161 tctgaacata acagaacctt ttcggaaata ttgatcagga acaaaagcga tatcggcatt
    11221 tgaaagcata cctgccccac caataaatag agcaggcatg catttcaaat acactattga
    11281 agtctagtca aaagatgaat atcgaccagt tttgtcttaa atatcgcctg atccagattg
    11341 cccactctag ggtgcgacgc gaacagcacg ttttcgtata ccattgggaa atatggctta
    11401 tccgtcatga gctgggtctg cacctttata taggcttctt cacgagtctt acgatcgagt
    11461 gaatagcgtc caatttgaat gtttttgttg acaatatcgt tgttgtagag cgcaccattc
    11521 attccggtcc cgatagagtc gccgccatag attatgcagg tatagtctgg atccatagta
    11581 ggtgtgaacc aggcggtcac caccatgtcg tacccatctc cagtcttgct ctgctgtatg
    11641 atctgcgcat tcaccattgg gactactttt atcaagtcaa agccaagagt cgtcttgacc
    11701 tggtctttta tattgttagc aattttcact cggtctgtga cgttacccac aaccaaggtt
    11761 accgattccg gccctaactt tgccctgaat tcgggcgaaa gcatggcaaa ttcggcttta
    11821 gccttttcct tgttgtagta ccaggtcttc tcattgaact ctgcaaaggg agtgcccgct
    11881 ggagcacctt cgacatagcc taggaatcct ggctgcatca tttgattggc tggcactgca
    11941 tagccattga agatatcgac ctgcttgatg aatggtactg gatttatcga atagaaaatt
    12001 gcccttctaa gatgagttga gtcaccttcg gtatactggc cagataaatc aagcttgccg
    12061 tttttcttct gcccaacaag atcggaattc atattgaaga cgccgtacca tattgtaact
    12121 ccaggtttcg tggtaataac aaggccgttt ctttccagat ttcctatatc gagcttagag
    12181 aggttgaagg ccatgtccac ctcaccggtt atcaagacgt tgttgacttg cggttgttcg
    12241 gacatagctc taaagacgat attcttaaaa gcaactgtgt tggcgtctcc ccaccaagct
    12301 tcgttccgcg taagctggac ttcctgtgga tttggccatg acacgaattt atagggcccg
    12361 gttccaacaa caatctttgt gccataatcc gcacctgcgg cttctacggt ctccttgtct
    12421 tctatgccag agcagtagtg cgcaagaatg ttgagcgctg gtccgtatgg agaagctgtc
    12481 atatattttg cacgatcttc ccatgaggtc aacgcgacag ttgcatattc aggatctttg
    12541 agcttgccaa aagtgatctt tactgtgtaa tcatctacct tcactacaga aagaaccgca
    12601 ctgaacatac tcttggaact cgacttcggg tgatgtagaa ttcgattgaa cgaaaagact
    12661 acatcatcgg cagtaaatgg attgccatta tggaatttta cgccttttcg gagcttgata
    12721 gtgtaaacag aaccgtcctt ggaaacgagt ccgttctgaa tcgttggtat agtctcggct
    12781 agaattggat aaaaggtatt atctgacttc attccaatca acgtttcatt gatctccctc
    12841 attatctgcg cttcgttcag agtcgtgtat atgtttggat cgagcggctg tggagcagca
    12901 gcaaggacaa agaccaaggt atcaggatct ctgttccgca aagttggccc tttctttgct
    12961 tgtgcaaaaa cgactccgcc caaggctagg cagataagta caatcgacaa aaatttcttc
    13021 atgtcaataa cctccttgaa gatattctat agctgcaacg cagcattaat ttattcacca
    13081 tataggtaag tttttctctt aaatgaaata agaatgcttg tataagtgtt tgttttcaag
    13141 tgaaagcggc aaacttgcca cctagcattg cggctgcatg aatcatccga tatatgcttc
    13201 tcatgtcggt agatctataa agaactcgcc ttcctcctct tcgttctagc cctggtatca
    13261 cggagacagc atccgctatg acagtagagg caaaatcgat ctcgagatcg taagctggcg
    13321 ctagagtaag tggcttgaaa gtatctttcc tgtctatggc ttgcatcgcc ccttcgacga
    13381 atttaggctt caggttgtcc tcgaagtaca ttttagctgc aaaccgccca aggccttgct
    13441 ttgtgcaaat aaatataggt ggaattggaa aatgctcttc tatttccttc tcaagaatat
    13501 catcgccgga cacaaaacat acgggcaccc cataatgaga agcgagcata gcatttatgt
    13561 ccgcttcgga catttcctgg ccattcaacc ggacacaata gatacacgag gacgaataac
    13621 tatgatccat caatccatgc aaggctccga tttttgcatg atatccaact agcataagaa
    13681 gatcgaaact ttcgtccagg ccttcgagca tatagaaagg acgcggatag ccgcggatca
    13741 agttaatacg cgaatcgcca aagcttctaa acggaatccc ctcgccccta gcatgagagt
    13801 cgcatatgca tatttctctg atttcatcgt tatacttcga tctatttatg ccttctacaa
    13861 tccaagacaa ttctttccat gcattctcat aaactgtttg gtgaccattg ccttccatat
    13921 cgctccatga gacagcacca tgaatgcctt ccatgtctac agaaataaaa accttcatgc
    13981 ttcatcctct gtgtgaacaa tctcttgcac aatagcgaga tatcgcctgc tagtcagtga
    14041 tttcattcaa aataaccttt cttgataact tcgcccctgc gaacgactaa acgcccacgg
    14101 cttattacat ctcgaatctc gagcccatcg gattccagta ccaaaatatc ggcgtccgct
    14161 tcttctctaa ttgtcccctt tctaccctcc aatccatagt ttcgagctgg attggatgtc
    14221 aaaggcaaaa gagcttcttc caatggcaga tgcttttgct gaacaagatt ccttagctcg
    14281 aacatcagtg tctcaggcgt tccgataccc attccggtaa ttctgctatg atctccattc
    14341 cactctggaa gcgaaccacc agcatcagaa ctgaaggtaa catgatccaa cagacctttc
    14401 tccctagcta gcattgcaaa atcggcggca ctcatgttct cagaattagc attcacttct
    14461 tttcgacatg tagcatcgac cattcctcca cccttggcca gttcgaatac ttggtccagc
    14521 aatttcggat ttttacgatt gatatgcgtc ggaacgaaca tagctggcct tatgcctgga
    14581 ttctttatca cctccataat aagatcgagc gcctgcggcc taatgccagt atggactgtg
    14641 attattccag gcttgttcgc aattagagcc gctacaaaga catccgacgc aagccttgca
    14701 agatccgcta catccatata tggtccgcga gtatccgata gagcaagctt taccccgatt
    14761 actggatcgc aaaacaaaat atccttggca accgagccag ttatgcttgg agaagggtac
    14821 caataagctc ctgttagcat gaaagccgtc atgccttcgg tattgaaagc cttagttttt
    14881 gcatagaggt cttcgagcga ccgagttaga gagtctgttc ctaagagccc tatggcagta
    14941 gtaacaccat ttagaaaatt cattgatatc tgaagttctg gagttctagt ttcaaatcct
    15001 ccttcgcctc cgccgcctat aaaatgctgg tgtccatcga tcaatcctgg aacgacaata
    15061 catcctgatg catcgatcgt ttcgacatgc gaaatcgagc tagatacatc gatattggaa
    15121 tcgacggaaa taatcctatc gcccccaatt agaatatccc taaatccaag cggatagggc
    15181 gcatatactt ctccattttt cagcagcaac atgctcatct tgtgctccat aatttgaatt
    15241 gaatttatta ttcttttgga ttactgataa ctaaataccc gctttcctgc atcgatatag
    15301 ttttctgccg gctcgctgcg gcgctttcct tttcgtccag atacaccatt tgcaccaaaa
    15361 catgcaaaat cgatatgagc ccggcaaaag aattggtcaa caacaaagaa ttagttatac
    15421 aacgtattac cttatctgct tcagaataca cagggcatgc agccgaatcc gtaaagagaa
    15481 agagcgaacc actcttttct ttgacgactt ttgccacatg ccttacctct aaagagtatg
    15541 gaggaaatgt catcagaaac atcacgtctt cgctcgaaaa agatttgaga accggagcgt
    15601 aatccgatac tgatcccgaa actacataca cttccttttc tagagaactc attaaagtat
    15661 atttaaaaat ttgggctaca taagcagttg ccccaaaagc gcttaaatat accctacgag
    15721 ctttctttat gccttctccc attatttcca gatcagccat ctttatattg gaaaaggtgc
    15781 tgcgtgtgtt gttgtattca ttttgaaata gcttttgcag acgtttttca tctggcagaa
    15841 tatccagctc actcaaagca atcttgtcgt aaggattcaa tctatgcctg atttcatctt
    15901 gaatttcatg cttgaattct tgataccctt caaaccctaa gctttttgca aatcggacca
    15961 gcgttgcgct acttacacca atggacctac ttattgtgtg gattgaggaa aaagcaacac
    16021 tctgttcgtg atccaacata taagagccta ttttctgata gctattcggc atttcaggaa
    16081 gcattttcct gatcttatca agaatcatga tgtttcaatt tctcctaatc caatcttggt
    16141 aacttttgtt tcagaattat cgcatcatta aatttatatg tcaacgttta tcggcaagat
    16201 tttttgattt tttgcaatca aggactcttg accgctaact cactgcagga taagtttttc
    16261 acaaggaggt acctttaatg aaacgattgt tgctgttgtt cgcaatccta gctgtcgtcg
    16321 ccgtcagccc agcgcttgca gaaaccgaag ccgaacttaa agctgcttac gaaaacgcgg
    16381 tcaaactcgc cactgctgct ccccaggact atacgcttaa ctggcaagcc gctcgcgcag
    16441 ctcgttctta tggggattat ctcgtcgtac accaaactac tggttggaaa gaaaccgcac
    16501 gcgcagcggc caaagaaggc atgaaatatg gagagattgc ctttaatctg aacccaagcg
    16561 gtatcgaagg atggtactac tatggccttt gcgtgggaac ctactcggat tgcgtcagta
    16621 tcgtcactgc cctcactgaa ggtcttaaag gcaaaaccca aatgggtttc gagaatgcct
    16681 ataaattcga taagacctac gacaatggcg gacctatcct tgcgcttggc cgcttctggc
    16741 aggttctacc cggcattgcc ggccaggatc gcaaaaaagc cgaaaaactc ttcgatgagt
    16801 acattgccct ctttggatcc tctcccaaag ctaatagcga cgcctggtat ttccgcggcc
    16861 aactctacaa agatacaaag cgcccagacc tcgccaaggc tgatctagaa aaagcagcct
    16921 cgatgggtaa tgaaaacgca aagaagctgc tggcggagat gaaata
//
LOCUS       DAOSOB010000043        16842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3432_length_16842_cov_73.073390, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000043 DAOSOB010000000
VERSION     DAOSOB010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 16842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16842
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3432_length_16842_cov_73.073390"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1148)
                     /locus_tag="PLS88_05800"
     CDS             complement(153..1148)
                     /locus_tag="PLS88_05800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOU06888.1"
                     /translation="MQGEKQPAPRNKTLTMNNLEREKYSKKLLIPDGSPLLPSKIINK
                     TIYSDLFNIINYLPKSFVDLLVIDPPYNLDKDFNGIRFSKSDDDKYMQYLESWFPKLI
                     NVVKPNGSIYICGDWKSTSCLYEIMKKYTIVRNRITWQREKGRGAKKNWKNTTEDIWF
                     GTMGDDYYFDVESVKQKRRVIAPYRENGAPKDWEETENGNFRLTYPSNFWDDISIPYW
                     SMPENTDHPTQKPEKLIAKLILASCPKNGIVLDPFVGSGTTSVVAKKLGRNYVGIEIN
                     EEYCCWTEKRLEIAEHNREIQGYSGGVFWERNTLSMQLDERKKANKKILKVMELT"
     gene            1297..>2467
                     /locus_tag="PLS88_05805"
     CDS             1297..>2467
                     /locus_tag="PLS88_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU06889.1"
                     /translation="MLAFKKFNLAELPELSAAKYDSRPALSMVVGSVYTYRDFERLSR
                     GIAQALASNGIKKGDRVALLAENSPQWVIAWFGIVRAGAIVVPILNDFMPKQIANIIQ
                     HSGAKIVFASNKLKAKLVEISPEPEIWDVSKDFEPSADSVELPAVEADDLAMIVYTSG
                     TTGISKGVMLSHRNILSNAIACKSIITLRRTDRLLSILPLAHTYEFTIGMVIPLLYGA
                     HIFYLDRPPSASALLPALKAVRPTIMLSVPLVIEKVYQSGIAPELNRMKLYKNKLMRP
                     LILRIAGMKLKKTFGGHLRFFGIGGAPLAADVEEFLKKAHFPYAIGYGLTETAPLIAG
                     CAPKATFLRSTGPVLKGVELRIADPKTSTGEGEIQARGPNIFKGYWKDEARTKEAF"
     assembly_gap    2468..2567
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2568..3158
                     /locus_tag="PLS88_05810"
     CDS             <2568..3158
                     /locus_tag="PLS88_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU06890.1"
                     /translation="PNIFKGYWKDEARTKEAFTEDGWFCTGDLGSIDNKGRLFVRGRL
                     KTLILGASGENIYPEEIESILNQMPEVEESLVVEEEDGLTALVCLKSEVLDNIGARLQ
                     DHLDAVGDLGSRVGQAIAQAEKSVTGSVNQALTDTEKAIERLLENIRKEANSRLAAFS
                     RIQRVKLHPEPFEKTPTQKIKRFLYGKKGEKPELAP"
     gene            complement(3226..3498)
                     /locus_tag="PLS88_05815"
     CDS             complement(3226..3498)
                     /locus_tag="PLS88_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06891.1"
                     /translation="MDPEMKKKIDETLAQVKEPQSDEPIVNLGLLEKVTYSEKEKTML
                     VKLAIGTPRWQCLACSAINGVVKAGIVRRAQEAFEKAFPELKIIVE"
     gene            complement(3638..4291)
                     /locus_tag="PLS88_05820"
     CDS             complement(3638..4291)
                     /locus_tag="PLS88_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06892.1"
                     /translation="MNNFLFFAIFALGVGATIQYFIGVKKNRWMGKSLSTQAEEILKP
                     KDTDYVNIGGAIGYNFSYKLKEPWKNAKGTFTMFPRHSLLYMPISLIVGNCDRFYMNI
                     FTDKKLVGEGHIIEKGHSRRAKIEGLEEMHKEEVKRAGKTFVLCWRSANLKDKLLQTL
                     DSMPDASSLTHFCCYPDNKTLFLYLKPKKGQISDNLRFFLAQCPTYFRQEKPSEPNK"
     gene            complement(4284..5216)
                     /locus_tag="PLS88_05825"
     CDS             complement(4284..5216)
                     /locus_tag="PLS88_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOU06893.1"
                     /translation="MRDTVFFLGKGGVGKTTLSSAFALALARRGSRVLVVSLDPAHNL
                     GDALQLKLNGEPKKVEPMLDALEVDLAYWVDRYLEESKEELKANYSYNTALNLDSFFS
                     ILKYSPGTEEYAVLWAIEDIRRRLAPKYDFVVFDTPPTALTLRFLSMPAISGLWVAEL
                     TKLRERILRDRQTIVRLNPQSPVAESCVDKEDDKVYGKLKNIRTRLTSLETLFAKQSF
                     MSVIVNPDELSVSEAIRIKDELSRLGIMLSAVCLNKKGLSQADWTLASEFNQLPLFAY
                     DFSPNGIHTREDLVHIGIEPFIDYFLGKRKVKNE"
     gene            complement(5226..5507)
                     /locus_tag="PLS88_05830"
     CDS             complement(5226..5507)
                     /locus_tag="PLS88_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06894.1"
                     /translation="MPKRFGHIIKDVFNTFAQVNREKATGMLDFELKELENIFALLIL
                     GGFVGLPSPPSPIAVELLPYMERELIILLSRSDLSQDPLGVLASMLEID"
     gene            complement(5751..7445)
                     /locus_tag="PLS88_05835"
     CDS             complement(5751..7445)
                     /locus_tag="PLS88_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOU06895.1"
                     /translation="MTTLIALLALAIYLIFYFAYGRVIRDKLLKSKEAPEAPSKRLQD
                     GVDYVPTNKYVLFGHHFASIAGAGPITGPAMAVAWGWLPGLLWIWLGNILLGAIHDYL
                     ALTASVRYDGRSVQFVAQDLMGKKAGTTFGWFTLFLCVLVVAAFGDIVAGQFAADGRV
                     FFSFVFFCVAAVIAGFAMYRSKLGLGWGSLIGLVLIILAFWFGDMLPVKMSKEIYFLI
                     IFVYIVLASTLPVNLLLQPRDYLSSFFLYFGLLVGGISALISAKPLDSIPAFTTFSAK
                     LIGPAGALQPSPFWPTVPLIIACGALSGFHSLVASGTSAKQLKEEKDGLFVGYGAMLV
                     EGFLATLVIISIASFGAAALGDKLMATPALGRFVNSFAMMVSTNLPFLPMSFMTLFTA
                     VWVSTFALTTLDTTNRLGRYIVQEMAEPLKEKNPSSYKFFSNKWVASILIAFLGIFLA
                     WSGGYSTMWPAFSGANQLLASVVMLTMAIWVKKKLNPKYVMSVLIPAAFLWITVTVAL
                     IWYEIVIVPVFFADASKTMNIVTGIIVGIINLFMLILNFIMINSFLKNYKLKPETA"
     gene            7652..8770
                     /locus_tag="PLS88_05840"
     CDS             7652..8770
                     /locus_tag="PLS88_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOU06896.1"
                     /translation="MEDQRLRKLAQLLVNYSTKVHAGDRVLIENSNLESDFVRLLIEE
                     VHAIGGLAFISLRDRRIERTLFMDAPEEQFDLQAEFESARMDKMDVYIGFTSVRNSFA
                     WQDLPASKIELYNSHVWKKVHIDRRIPHTRWVVLRYPSAAMAQNAGMSEDAFEKFYFD
                     VCTMDYEKMSRAMDPLVELLQKSDRVKIIGPETDLEFSIRGLPAIKCDGTMNIPDGEV
                     YTAPVRDSVNGILSYNTPNEKDGFKFENIVFEFKDGKIVQARANDTERINKILDIDDG
                     ARYLGEFSFGINPYITRPILETLFDEKIAGSLHITPGNSYDDCFNGNRSALHWDIVLL
                     QDKASGGGEVWIDGRLIRKDGLFVLPELKGLNPEALKS"
     gene            complement(8786..9016)
                     /locus_tag="PLS88_05845"
     CDS             complement(8786..9016)
                     /locus_tag="PLS88_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOU06897.1"
                     /translation="MADRSNRLPQNAKGKWYVDASCIGCGLCSVTAPDIFTLGDDGQA
                     YVTRQPQTKEENELAIEAMNDCSAQSIGDDGE"
     gene            complement(9189..9608)
                     /locus_tag="PLS88_05850"
     CDS             complement(9189..9608)
                     /locus_tag="PLS88_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOU06898.1"
                     /translation="MNNLVLYDKSPFDLIESFFNNEDWLTQSFRTPAIDVYEEGDKYT
                     IEADLPGLTEKDIKLEVRNGQLMLATAKREKSEEKSKKGRWIRRERREYQFQRSFTLP
                     EEVDPEKIEAHFKNGVLEVVLPKKPEAAPKSIEVKIN"
     gene            10047..11042
                     /locus_tag="PLS88_05855"
     CDS             10047..11042
                     /locus_tag="PLS88_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU06899.1"
                     /translation="MKRLIIAGLVLILSLTVAFGQQKLGVYTTLDEPLAKALFDEYQK
                     ETGIILNWQRLSGGEVEARLEAEKANPQASIWVGGVGLNHISAKLKGLTAPYKSKMLE
                     NTPLQFRDPENYWVGLYIGPICFVTNNKVAKEQGIKPPTSWADLLKPEYKGKIRVANP
                     TTSGTAYNVITTLRYIFGGDEEKVFDYLNKLDKNIDQYTKSGAAPGKSVAIGEIPVAI
                     GYAHDNVKLKVEGADVTITFPSEGTGYEIASMSIVKGGPDPVNAKKLYDWVLSPKAQE
                     IIASWYVIPVSKIAKKNPQAFSLSEIKTVNQDVVWDAANKDRLLERWVKEIGSKR"
     gene            11176..13380
                     /locus_tag="PLS88_05860"
     CDS             11176..13380
                     /locus_tag="PLS88_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU06900.1"
                     /translation="MFTKKKIIMISVVCLIFAIGEYWIFSTIQNTFEKNTLKETKALV
                     KGITSFAPSNDEALSDWIIELSDIYPDNRFFAIKGVPGEEGSELIGGDEALLDLWQLP
                     EYAEAASYATESVSYLEIYKWPKIVHFKGVPYMLMVAPIPDAENSEARATLLAAINST
                     GYVSFSRLVDALALIAFVLFGVGFGIATFSRDPISGYAIVVLAAIVLAFVAYPLLEAV
                     RLTLIKDGKFSFNVWKTILSNRQYLQALWGSIQLGIVTATISTFIGFVYAFAVARTSM
                     KGKKLVTTLATMPVISPPFSLTLSIILLFGKNGIVTKQWLGLQNINIYGLWGLALAQT
                     ISMFPIAFMTMQGVLEAIDSTLEDASLDLNASRWYTFRHVTLPLAAPGLLSSWLLVFT
                     NSLADFANPLLLSGSYRVLSVESYIEAIGMNRLNNGAALSLLLLLPTLTAFLAQRRWV
                     NRKSFVVVTGKPSTRLSDLASSGVRKALTGFVILVSAFIVGLYGTIVAGCFVKNWGID
                     YSFTLANIGEALTRGKQSLVSTITLAGIATPIAGILAMVAAFILVRKNFPGKRILESL
                     IMAPFAIPGTLIGISFILAFNKPPLLLVGTGAILVINYVVRELPVGVEGGIAALRQID
                     PSIEEAAADLGADQATIFRTIILPLIRPAFISSMSYTFVRSMTAVSAIIFLISAKWYH
                     ITIQIYNFSENLRFGLASVLATTLIIIVLAVFGLMRLLVRQSEYLEKTITTQ"
     gene            13443..14564
                     /locus_tag="PLS88_05865"
     CDS             13443..14564
                     /locus_tag="PLS88_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06901.1"
                     /translation="MTAKSVPVTLSHVTKIFKDPKGKGEVHAVEDADFEVKPGELVTL
                     LGPSGCGKTTTLRMIGGFELPTKGKIIIGDTDVTFLPPNGRQTATVFQSYGLFPHMDV
                     FDNVAYGLKIRKLAKDEIEKRVTDILDLVGLSELMHRAPGRLSGGQQQRVALARSLVV
                     EPQVLLLDEPLSNLDALLREQMRVEIRRIQKSLGITAIYVTHDRIEAMSLSDRIIVMR
                     DGKIQQIGTPSGIYVEPDSVFVAGFVGKVAFFHAQLEDFEPDGRAICRLGQKKVHAGS
                     AVRGLKAGDEVELMARPESLRIVSQEEAVASGKVFAKVYLGSNVEIYVKTETGELLIE
                     IEDPLGKVIPSEGETVWIGFNEKKVRALPAEEQTEGQSK"
     gene            complement(14630..15235)
                     /locus_tag="PLS88_05870"
     CDS             complement(14630..15235)
                     /locus_tag="PLS88_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOU06902.1"
                     /translation="MAKRYLVCITGASGAIYSIRLLSALAASGAMLHVVTSRWGARVL
                     LEETGRPLGYWLGRLQAKGGPGGGPALIAVHENNDLLAPISSGEFRLNGTVIAPCSMG
                     TLGAIASGNCSNLIHRAGAVALKEGWPFIVVPRETPLSLVSLRAMAQLKEAGAVILPA
                     SPSFHNKPADIQSLVDTVIYRILDHLDIVDQTMTRENQDKP"
     gene            15429..16124
                     /locus_tag="PLS88_05875"
                     /pseudo
     CDS             15429..16124
                     /locus_tag="PLS88_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271504.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            16244..>16842
                     /locus_tag="PLS88_05880"
     CDS             16244..>16842
                     /locus_tag="PLS88_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06903.1"
                     /translation="MISARLAWRNLGLHRKKSLIIGLIMGLGILILFVGNSLIDTTIS
                     GLRRMFVEGYTGDLMVTGPTEFSTTVLGETAGSEEVLPHIAQIKKYEDFLKSRQDVAS
                     ILPILSGQVSFGIGEEELGSGYCFGVNISDYRAFFPDNVSLLSGEWPEESDEPWVLLS
                     EPTYALLQQSSNTEVKPGTKIIMTALANSAGTVIREVSV"
BASE COUNT         4498 a         3679 c         3936 g         4629 t
ORIGIN      
        1 gtggtaatcc ggtcgatgtg atctatcgcg atgcgattgc cgattccaat cacgaggtgc
       61 tcaaaaatgc ccgatgtcct gtgctctttg ttaagccccc gataggagat gatctgtata
      121 ggtctatata acattgaatt gggtgggctt acttaagtca gttccatgac cttcaatatc
      181 tttttatttg ctttctttct ttcatctagt tgcatgctca aagtatttct ttcccagaat
      241 acaccgcctg aatatccttg tatttcccta ttatgttcag cgatttctaa tctcttttca
      301 gtccagcagc aatattcttc attgatctct attccaacat aatttctgcc aagttttttg
      361 gccacaacag atgttgtacc agaaccaacg aatgggtcta ggacgatacc atttttaggg
      421 cagcttgcaa ggattaattt tgcaattagt ttttctggct tttgtgtcgg atgatctgtg
      481 ttttctggca ttgaccaata cggtatagaa atatcgtccc aaaaattact ggggtatgtt
      541 aagcggaaat tgccattctc tgtttcttcc caatcttttg gtgctccatt ttctcgatac
      601 ggagctatca ctcttcgttt ttgctttact gattccacat caaagtagta atcgtctccc
      661 attgttccaa accaaatatc ctctgtggtg ttcttccaat tcttctttgc tccacgtccc
      721 ttttctcttt gccaggtaat tctgtttctt acaatggtat atttcttcat tatttcgtat
      781 agacaagaag tgcttttcca atcaccacat atgtatatcg atccgttagg cttaacaaca
      841 ttgatcaatt tcgggaacca actttcaaga tattgcatgt atttatcatc atcacttttg
      901 gaaaatctta ttccattgaa atctttatcc aaattatagg gagggtcaat gacaagcaaa
      961 tcaacaaatg actttggcag atagttaata atattgaata ggtcagaata gattgtctta
     1021 tttattattt tgcttggaag taatgggctc ccatcaggaa taagaagctt tttactatat
     1081 ttttctcttt ctaaattatt cattgtgaga gtcttatttc tcggggctgg ctgtttttct
     1141 ccttgcatat gactacttct tcctattata agaataaagg ttaccttaga gcaaggcttg
     1201 ccactatgta attcaataaa aagcgagccc cctatcccaa tactcttttt cacagtaaac
     1261 tttgttcttc ttaggtttga aatataaaga gggaatatgc ttgcctttaa aaaattcaat
     1321 cttgcagagc taccagagct aagcgctgcc aaatatgact ctaggcctgc tctatcgatg
     1381 gtggtcggat cggtatatac ctatcgcgat tttgaacgct tgagtagggg catagcgcag
     1441 gctctagcgt caaatggcat taagaagggc gatcgcgtgg ctttgctggc cgagaattct
     1501 cctcaatggg tcatagcttg gtttggaata gtgagagcgg gagccatagt tgttcctatc
     1561 ttgaacgact tcatgccaaa gcagatagcc aatattatcc agcattcagg cgcgaagatc
     1621 gtttttgcat ccaataaatt aaaggcaaag ctcgttgaga tttctcctga gccagagatt
     1681 tgggatgttt ccaaggattt cgagccatcg gctgattctg tcgaattgcc agctgtcgaa
     1741 gcggatgatc ttgcgatgat cgtctatacc tcgggtacca ctggtatttc caaaggtgtg
     1801 atgctcagtc atcgcaatat cctgtccaac gcgattgcat gcaagagcat cataactctg
     1861 cgaagaacag atcgtctttt atcgattctt ccgcttgcgc acacctatga atttacgatt
     1921 ggcatggtga ttcctctctt gtatggtgct catatctttt atctggacag gccaccttca
     1981 gcatcggcgc ttcttccagc gctcaaagct gtaagaccta cgattatgct gtcggtaccg
     2041 cttgttatcg aaaaagtata ccagagtggc attgctcccg agctgaatag gatgaagcta
     2101 tacaagaaca agctcatgcg gcctttaata ctccgcatag cgggaatgaa gcttaaaaag
     2161 acctttggtg ggcatctgcg cttttttgga attggcggag cgccccttgc ggccgatgtc
     2221 gaggagtttc ttaagaaagc tcattttcct tatgcgatag gttatggcct tactgagacg
     2281 gctcctctta ttgcgggctg cgctcccaaa gcgacttttc tgcgttccac tggtccagtg
     2341 ctaaagggag tagagttgcg catagccgat cctaagacca gtaccggaga aggcgaaatc
     2401 caagcaagag gcccaaatat cttcaaaggc tattggaaag acgaggctcg aacaaaagaa
     2461 gctttcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncca aatatcttca
     2581 aaggctattg gaaagacgag gctcgaacaa aagaagcttt cacagaagac ggatggttct
     2641 gcacagggga cctagggtcg atcgacaata agggtcggct cttcgtgcga ggacggctca
     2701 agacattgat tctaggtgcg tcgggcgaaa atatctatcc cgaagagatc gaatcgattc
     2761 tcaatcagat gcctgaggta gaagaatctc ttgtagtgga agaagaggat gggctcacgg
     2821 cgctagtatg cctaaagagc gaagtactag ataatattgg agcacgtcta caggatcatc
     2881 tcgatgccgt tggagatttg ggttcgcgtg ttggacaagc tatcgctcaa gccgaaaaat
     2941 ccgttacagg cagcgtaaac caagccttga cagatacgga gaaagctatc gaacgccttc
     3001 tggagaacat ccgcaaggaa gcgaactcaa ggcttgccgc tttttcgcgc attcaacgcg
     3061 taaaattaca tcctgaacct tttgaaaaaa ctccaactca gaagatcaag cgctttctat
     3121 atggaaagaa gggtgaaaag ccagagcttg ccccataaat atgcgagcat agcggcggct
     3181 acaatattta ttttgtcata gattatagat tgctgttcta ctttgttatt ctacgattat
     3241 cttcaattca gggaaggcct tctcgaatgc ctcttgagct ctgcgcacta tgcctgcctt
     3301 tacgactcca tttattgcgg agcaggcgag gcattgccac cgaggggtgc cgattgcgag
     3361 tttgactaac atggtttttt ctttctcgct ataggtgact ttctctagaa gcccaaggtt
     3421 tacgataggt tcatcggact gtggttcttt tacttgggcc agggtctcat cgatcttctt
     3481 tttcatttcg ggatccatag gcgtctcctt ttactccctt tgcatgcttt taccttatat
     3541 gacaatccag caggcgcgcc atattccgag tgcgggtttt cgcaatcttc ccactcttta
     3601 aggcttcctt aaggctacct agctcaagta tagcagccta tttatttggc tcgctaggct
     3661 tttcctggcg gaaataggtt gggcattgtg ccagaaagaa tcttagattg tctgatatct
     3721 ggcctttttt gggtttcaag taaaggaata atgtcttatt gtctgggtag cagcagaagt
     3781 gggtaagcga tgaggcatcg ggcatggaat cgagagtctg gagaagcttg tcttttagat
     3841 ttgccgatcg ccagcagagc acaaaggtct ttccagcgcg ctttacttcc tctttatgca
     3901 tttcctcgag gccttcaatt ttggccctgc gagaatgtcc tttttcgatg atatggcctt
     3961 ctcccaccaa ttttttatcc gtgaagatat tcatataaaa gcgatcgcag ttgcccacaa
     4021 tgagcgaaat gggcatatat aagagagaat ggcgagggaa catcgtgaaa gtgccctttg
     4081 catttttcca tggttctttc aacttataac taaaattata gccaatcgct ccgccaatat
     4141 tgacataatc agtatccttt ggtttcaaga tttcctcggc ttgagttgac aagctctttc
     4201 ccatccagcg gtttttcttt actccaatga aatactgaat agtcgcccca acgccaagtg
     4261 caaaaatcgc gaaaaacaaa aaattattca ttttttactt tcctcttgcc gagaaaatag
     4321 tctatgaatg gttctatgcc gatatgaacc aagtcttctc ttgtatgtat gccatttggc
     4381 gaaaaatcat aggcgaatag gggcagttgg ttaaattcag aggcaagcgt ccagtctgct
     4441 tgcgaaaggc cttttttgtt aagacaaaca gcggagagca tgattcccag gcgagacaac
     4501 tcgtctttga ttctaatggc ttccgatacc gaaagctcat ctgggtttac gatgacgctc
     4561 ataaaacttt gttttgcaaa caaggtctct agcgatgtga gtctggttcg tatgtttttg
     4621 agctttccat agaccttatc atcttctttg tccacacagc tctctgcgac tggcgactga
     4681 gggttcaagc gaacaatcgt ctggcggtcg cggagaatcc gctcacgaag ctttgtaagc
     4741 tctgccaccc aaaggcctga aatagcaggc atagaaagaa agcgaagagt aagagccgtt
     4801 ggcggcgtat cgaacaccac gaaatcgtat ttaggtgcta gtctgcggcg aatatcttcg
     4861 attgcccaca atactgcata ttcttctgta cctggtgagt actttaagat actgaagaaa
     4921 gaatcaaggt ttaaggctgt attgtaggaa tagttagcct tgagctcttc tttgctctct
     4981 tcgagatatc tatccaccca atacgctagg tcgacttcga gcgcatcgag cattggttct
     5041 actttttttg gctctccatt gagttttaat tgtagagcat ctccaaggtt atgcgcagga
     5101 tcgagcgata ctacaagaac tctactgccc cttcttgcca aggctagggc aaaggcactg
     5161 gagagtgtcg ttttgcctac gcctcccttc ccaaggaaga atacagtatc gcgcacaaaa
     5221 actccttagt ctatttcgag catactggcc aatacaccaa gaggatcctg agaaagatcc
     5281 gacctggaca gaagtatgat tagctctctt tccatgtatg gcaataattc cacggcaata
     5341 ggcgatggcg gcgaaggcaa gcccacaaag cctccaagta taagaagcgc aaagatattt
     5401 tcaagctcct tgagctcgaa atcgagcatc ccagttgctt tttctctatt tacctgagca
     5461 aaggtattga acacatcttt gattatgtgt ccaaagcgct tgggcatttt tgtttcctta
     5521 ggattcatat atagacaact tattttaggc ttaatctaaa ttacctaact atagcgcgct
     5581 tagagcaaga ttggcaagct tctagtttct gtgtttcatc tccgcagaac aaatttatcc
     5641 actatataat aaaagagccg gaaagcacat gccttccggc tctttgtttt gtcgccatcc
     5701 gcctgtcctt ttaatccaac ggatctaaga caggcagaaa ttgccattta tcaggctgtc
     5761 tctggcttca gcttatagtt ctttaggaac gagttgatca ttataaaatt gagtatgagc
     5821 atgaagaggt tgatgatgcc tacgataatt ccggtgacta tgttcatcgt ctttgaagca
     5881 tctgcgaaga atacgggcac aatgacgatc tcataccata tgagcgccac cgtgacagtt
     5941 atccatagaa aagcagccgg aatcaatacc gacatgacat atttggggtt gagcttcttc
     6001 tttacccaga ttgccatggt gagcatgaca accgaggcca ggagctgatt ggctccggag
     6061 aaggcaggcc acatcgtgct ataaccgcca gaccacgcaa ggaaaatccc caaaaaggcg
     6121 atgagaatcg atgcaaccca cttgttcgag aagaatttat agctacttgg attcttttct
     6181 ttgagaggct ctgccatttc ctggacaatg tagcggccaa gcctattcgt cgtatcgagg
     6241 gtcgtcaggg caaaggtaga tacccatact gccgtaaaga gtgtcatgaa actcataggt
     6301 aagaagggca gatttgtgct gaccatcatt gcaaaggaat ttacaaagcg gccaagcgca
     6361 ggagtggcca tgagcttatc tccaagggcg gcggctccaa acgaggcgat agatatgatg
     6421 acaagagtag caaggaatcc ttcgaccagc attgcaccat atcccacaaa aagcccatcc
     6481 ttctcttctt tgagctgctt agcgctcgtg ccagatgcca caagagagtg gaaacctgaa
     6541 agcgcgccgc aggctattat gagaggaaca gtaggccaga aaggcgaagg ctgaagagcg
     6601 cccgcaggtc caattagctt ggcgctaaag gtggtaaagg ccgggatcga atcaaggggc
     6661 tttgcactta tgagcgctga aatacctcct acaagcagcc cgaaatacag gaagaaagaa
     6721 gagaggtaat cgcgcggctg caagagcagg ttaaccggca aagtcgaagc taacacgatg
     6781 tacacgaaga tgataaggaa atatatttcc ttgctcatct tgacggggag catgtcgcca
     6841 aaccagaagg ctaggattat cagcaccaag cctatcaagg aaccccagcc taggccaagc
     6901 ttcgagcgat acatggcaaa gcccgcgata accgcggcta cgcagaaaaa cacgaatgaa
     6961 aagaagactc gtccatccgc agcgaactgg cctgctacaa tatcgccaaa ggctgccaca
     7021 accagcacgc agaggaagag cgtaaaccag ccaaaggttg tgcctgcctt tttgcccata
     7081 aggtcttggg caacgaattg caccgagcgg ccatcatacc tgacagatgc cgtcagcgca
     7141 agataatcgt ggattgcgcc caatagtatg ttgccgagcc aaatccacaa tagtcctggc
     7201 agccatcccc aagccactgc cattgccggc cctgtaatcg ggcctgcgcc tgcaatcgaa
     7261 gcaaagtggt gaccaaacag gacatacttg ttggtgggca catagtcgac tccatcttga
     7321 agccttttgg atggagcttc tggcgcttct ttgctcttga gtagcttgtc tcggattacc
     7381 cgaccatatg cgaaatagaa tatcaggtat atcgcgaggg caagcaaggc aatgagagtt
     7441 gtcatgcagt cctcctatga gaaaacggat attttatttt aactacagtt tcttgacggc
     7501 gtcaatggtt ttttattcta tatatgtgta ctattaaaaa agatgcatac cttttcagag
     7561 tttttcgata tacgttatta tatagggtgc tattcaagag agattatata gatgcttatc
     7621 aagatcaaga ttattcagaa agggaacata aatggaagat caaagactta gaaaactggc
     7681 gcagctcttg gttaattatt ctactaaagt gcatgctggc gatagagtgc tcatcgaaaa
     7741 ctcgaacctt gaatcggact tcgtgcgcct tctcatagaa gaagtgcatg ctataggagg
     7801 acttgctttc atttctttga gagacagacg gatcgaaagg acactcttta tggatgcacc
     7861 ggaggaacag ttcgatctgc aggcagaatt cgaaagcgct cgcatggaca agatggatgt
     7921 atatatcggt tttactagcg ttcggaattc atttgcatgg caagacttac cggcgagcaa
     7981 aatcgagctc tacaatagtc atgtctggaa gaaagtccac atcgatcgtc gaataccgca
     8041 tacccgctgg gttgtgctcc gctatcctag cgccgccatg gcgcaaaatg cggggatgag
     8101 cgaagatgca tttgaaaaat tctatttcga tgtatgtaca atggactacg aaaaaatgtc
     8161 tcgcgccatg gatccgcttg tggaacttct tcagaaaagc gacagagtaa aaattatagg
     8221 acctgagacc gatctcgagt tctcgataag aggtcttcct gctatcaaat gcgatggaac
     8281 catgaacatt ccagacggag aagtctatac agctccggtg cgcgattcgg taaatgggat
     8341 attgagctac aatactccaa acgagaagga tggttttaaa ttcgagaata ttgttttcga
     8401 attcaaagac ggcaagatcg tccaagctcg cgctaacgat actgagcgga taaataaaat
     8461 acttgatatc gatgatggag ctcgctatct tggtgagttt tcttttggta tcaatcctta
     8521 tataactcgc ccaatcttgg aaacgctctt cgatgaaaaa atagcaggct ctttacatat
     8581 cacgcctgga aactcatacg atgattgttt caatggcaat cgctcggcgc tgcactggga
     8641 catagttctt ctgcaagata aagcctcggg cggcggagaa gtatggatcg acggtaggct
     8701 gatccgaaaa gacggactct ttgtcttgcc tgagttgaaa ggattgaatc ccgaggctct
     8761 caagagctag tgatgactgg agagcttact cgccgtcatc tccgatcgac tgagcagagc
     8821 aatcgttcat ggcttctatc gctagctcgt tctcctcttt tgtttgaggc tgacgagtga
     8881 cgtaagcttg tccatcgtcg cctagggtga agatgtctgg agctgtaaca gagcataagc
     8941 cacagccaat gcaggatgca tcgacatacc atttaccttt tgcattttga ggaaggcgat
     9001 tggatctatc cgccataata tctcctttgc cagcaaagcc ggttatgtaa atctagaatt
     9061 cgttcagcat gataacacaa taaggctgag aactgaaagc cgtgctataa aaaaaccggg
     9121 cctaaagccc ggcttttata aggaagatta agaagcacat ctaagaagcc gaagcaaaga
     9181 agttcagttt agttgatttt tacctcaata ctcttgggag cagcttcagg tttctttgga
     9241 aggacgactt ccagcacacc attcttgaag tgagcctcga tcttttcagg atctacctct
     9301 tcgggcagag taaagctgcg ctggaactga tattctctcc gctccctgcg gatccaccga
     9361 cctttcttgg atttctcttc gctcttctct cgtttggcgg tggcaagcat aagctggcca
     9421 ttgcgaactt ccaactttat atctttttcg gttaagccgg gaagatcggc ttcgatggtg
     9481 tatttatcgc cttcttcgta tacgtcgata gctggagtgc ggaagctctg cgttagccaa
     9541 tcttcgttgt taaaaaagct ctcgataaga tcaaatgggc ttttgtcata caaaactaga
     9601 ttgttcatag ttaccctcct attatttagg atcagcaggt ttagatccac acaggattct
     9661 atagcctgtc tggagccctt ttcggcgctc atctataata tgagcaagaa gcgtgccaaa
     9721 atcatataaa ttaccatcaa aataataaat cattatgcaa taataaaatg tcaattatac
     9781 ctgaacagca atacgactgg aaatgaatat caagagctct tatatgggtt tcaatgagac
     9841 atagctataa gattagccct tggatttcta tttccgaatg atacatttgt attgatatga
     9901 gacagaagga ctgtataata atgatacaat ttgccttctt aggtatctga gctgttggtc
     9961 aaatcttatc tactataata tttcgccttg cggaaatcta agtcatggca tacactatat
    10021 tcgtaaagtt cttttaggag gtttccatga aacgtctaat tatcgcaggg cttgtgctaa
    10081 ttctcagtct gactgtggca tttgggcagc agaaactcgg cgtgtataca acactggatg
    10141 agcctctcgc taaagcgcta tttgatgagt atcagaaaga aactggaata atactcaatt
    10201 ggcagaggct ttcaggcggt gaagtagaag ccaggctcga ggcagagaaa gcaaatcctc
    10261 aggcttcaat atgggtggga ggagtagggc tcaatcacat tagcgccaaa ctcaaaggtc
    10321 tgacggcacc ttataaatcc aagatgctcg agaatactcc tctgcagttt cgcgacccgg
    10381 agaactattg ggtagggctc tatataggac ctatctgttt cgtgaccaac aataaagttg
    10441 cgaaagaaca aggaattaag cctcccacat cctgggccga tctattgaaa cccgaataca
    10501 agggcaagat acgcgttgca aatccaacga cctctggtac ggcctacaat gttataacta
    10561 ccttgcgcta tatattcggc ggcgatgaag agaaggtatt cgattatctg aataagctcg
    10621 acaagaacat cgaccaatat accaaatcag gcgccgcgcc cggcaagagt gtggctatag
    10681 gagaaatccc tgtggcaatc ggatatgcac acgacaatgt caaactcaag gtcgagggtg
    10741 cggatgtcac gatcaccttc ccctcggaag gcaccggata tgagattgcc tcgatgtcca
    10801 tagtaaaagg cggccctgac ccggtaaatg ccaagaagct ttacgattgg gtgctttctc
    10861 caaaggcgca ggagattatc gcatcgtggt atgttattcc cgtatcgaag atcgccaaga
    10921 aaaatcctca agccttcagt ctcagcgaga tcaagaccgt aaatcaagat gtggtatggg
    10981 atgcagctaa taaagatcgg cttctcgagc gatgggtcaa ggagatcggt tccaaacgct
    11041 gatttttatt tctgtgtgag atcgcaagcc ggaatataag aaaatgagcc ggaccctgag
    11101 gaatggtatg ttccccagag gtccggttct tggtattctt ggttctgata gtcaggaaga
    11161 aaagaggatc tccaagtgtt taccaagaaa aaaattataa tgatttctgt ggtctgcctt
    11221 atcttcgcca taggagaata ttggattttc tctacgattc aaaatacttt cgaaaagaac
    11281 acgctgaaag agacgaaagc acttgtaaag ggaataactt cttttgcgcc ttcgaacgat
    11341 gaagctctct ctgattggat aatagagctt agtgacatct atccggacaa tcgcttcttt
    11401 gcgataaagg gagttcctgg cgaggaggga agcgaattaa ttggcggaga tgaagcgctc
    11461 ttagacctat ggcagcttcc ggaatatgcg gaagccgcaa gttatgctac ggaatcggtt
    11521 tcttaccttg aaatatacaa atggccgaaa atcgtccatt ttaagggtgt cccctacatg
    11581 cttatggttg ctcctatacc cgatgcggag aatagcgaag ctcgggctac tcttcttgca
    11641 gccataaatt ctactggcta tgtgtctttt tctaggcttg tagatgctct tgccttgatt
    11701 gcctttgttt tattcggcgt tggctttggc attgcgacat tctcgcgcga tccaatcagc
    11761 ggttacgcaa tagtagtttt ggctgcaata gtgcttgcat tcgttgcata tccactctta
    11821 gaggccgtgc gccttacctt aataaaagat ggcaaatttt ctttcaatgt atggaagacc
    11881 attctttcaa atcgtcagta tttgcaagcg ctctggggtt ctatccagct tggcattgta
    11941 accgcaacga tttcgacttt cataggcttt gtatatgctt ttgcggtggc gcgcaccagt
    12001 atgaaaggca aaaaactggt aactactctt gcgaccatgc ccgtaatatc accaccattc
    12061 tccttgacgc tgtcgattat cctcttgttt ggcaagaatg gtatcgtaac caagcaatgg
    12121 ctcggccttc aaaatatcaa tatatatggt ttgtgggggt tggccttagc gcagacaatc
    12181 agcatgtttc ctatagcatt catgactatg cagggcgtat tagaggcgat tgactccacg
    12241 ttggaggatg cttcgctcga tctcaatgct tctagatggt acacattcag gcacgttaca
    12301 ttgccacttg ctgcgcctgg tcttctttcg agctggctac ttgtattcac caattcgctt
    12361 gcagattttg caaatcccct tcttctatct ggttcctatc gagtgctatc ggtagagtca
    12421 tacatcgaag cgataggcat gaataggctg aataatggtg cggctctgtc cctacttttg
    12481 ctcttgccta ctttgacggc atttctggcg cagcgccgct gggtgaatcg caagtctttt
    12541 gtggttgtta ccggcaagcc ttctacgagg ctctctgacc tcgcatcttc tggagtaagg
    12601 aaggcgttga caggctttgt aattttggta agcgccttta ttgtcggttt gtacggtacg
    12661 atagtggctg gttgctttgt caagaattgg ggtatcgatt attcctttac tcttgctaat
    12721 atcggcgagg ctttgacaag aggaaagcaa tctcttgttt caacaatcac actggctgga
    12781 attgccactc ctatcgcggg aattcttgcg atggtggcgg ctttcatact cgtgcgaaag
    12841 aattttccag ggaaacgaat cctagaatct ctcatcatgg cgccttttgc cattcctggt
    12901 actctcatag gaataagctt tattctggca ttcaacaagc ctcctttgct tctagtcgga
    12961 acaggagcga tattggtaat aaattatgta gtgagagagc tgccggtagg cgtggagggc
    13021 ggtattgccg cgctaaggca gatcgatcct tcgatagaag aagctgcggc tgacctcgga
    13081 gccgatcaag caacgatatt ccgtactatt attttgccgc tgataaggcc ggcatttatt
    13141 tcgagtatgt cctatacttt tgttcgctcg atgacggctg tttcagcgat catattcctc
    13201 attagcgcga aatggtacca tatcactata cagatataca atttttcgga gaatttacgc
    13261 tttgggctcg ctagtgtcct tgctacgaca ttgatcataa tagtgctggc tgtctttgga
    13321 cttatgagac tcttggttcg acagagcgag tacctcgaaa aaacgataac gacccagtga
    13381 tggatagcga tcggagtcgt tcgactgcag agcagggttc attttatagg aaagggtaga
    13441 atatgacagc aaaatccgtg cccgtgacac tttctcatgt gactaagata ttcaaagacc
    13501 cgaaaggtaa aggcgaagtg catgctgttg aagacgctga cttcgaggta aagccaggtg
    13561 agctggtgac cttattgggg ccttccggat gtggcaagac taccacgctt cggatgatcg
    13621 gaggctttga attgccaacc aagggcaaga tcatcatcgg ggatacagat gtgacttttt
    13681 tgccgccaaa tggtaggcag acggctaccg tttttcaatc ctatggcctc tttcctcata
    13741 tggatgtgtt cgacaatgtt gcatatgggc tcaaaatacg aaagctagcc aaagatgaaa
    13801 tagaaaagcg agtcacggat atcctggatc tcgtagggct ttctgaactc atgcacagag
    13861 ctcctggtag gctttctggt ggtcaacaac agcgagttgc actggcgaga tcgcttgtgg
    13921 tggagccaca ggtgcttctg ctcgatgagc ctctatcgaa tctcgatgct cttttgcgcg
    13981 agcagatgag agtcgaaata cgccgtatcc agaaatctct tggaattacg gctatctatg
    14041 taacgcacga ccgtatagag gctatgagtc tttctgatag aataatcgtg atgcgcgatg
    14101 gcaagattca gcagatagga actccaagcg gtatatatgt agagcccgat tccgtattcg
    14161 ttgcgggttt tgtgggcaag gttgcttttt tccatgctca gctagaggac tttgaaccag
    14221 atgggagagc tatatgtaga ctagggcaaa aaaaggtaca tgctggttct gccgtaagag
    14281 ggctcaaagc aggggatgag gtggagctta tggcaaggcc cgaatcgctg cgcattgtca
    14341 gccaagaaga ggccgttgcc tcagggaagg tctttgcaaa ggtctattta gggtcgaatg
    14401 tagaaatcta cgtaaagaca gagactggtg aactgcttat agaaatcgaa gatcctttag
    14461 gtaaggtaat tccatccgaa ggagaaaccg tgtggatcgg tttcaatgaa aaaaaggtta
    14521 gagcgctccc ggccgaagaa caaacagaag ggcaatcgaa atagtatcaa tcttgatggg
    14581 caaacattaa ggattttagg cgattacttt tctaagattg acacagaaat catggtttgt
    14641 cttgattttc tcttgtcata gtttggtcga ctatatcaag atgatcaagt attcggtata
    14701 tgacagtatc gacaagcgat tggatatcgg cgggcttatt atggaagctg ggagaagctg
    14761 gaagaatcac agctccagct tccttgagtt gggccatcgc gcggagcgat acaagagaaa
    14821 gtggcgtctc tctaggcaca acaataaaag gccaaccttc ttttagagcg acagctcccg
    14881 ccctatggat aagattggag cagtttccgc ttgctatggc acctagcgtc cccatggaac
    14941 aaggtgcaat aactgtgcca ttaaggcgga attcacccga tgaaatggga gccaataaat
    15001 cattgttctc atgcacagca attagagcag gtccacctcc tggtcctcct ttagcctgta
    15061 gtcttccaag ccaatagcct agtgggcggc ctgtctcttc gagaaggact cgtgcgcccc
    15121 acctgctcgt cactacatgg agcatggcac cgcttgcagc aagcgcggag agtaatcgta
    15181 tggaatagat tgcgccagaa gcgccggtga tgcagacgag gtagcgcttt gccatgcttg
    15241 ctattttagc ctaggtaaat atctagcgca atgccaaata agaaaaggat agaaataacc
    15301 tgatttaagg tatacccagt cagattgtaa ttgttgttat aattattcat ttctttttca
    15361 gctcattctt atggaaacaa ttgtttctaa acattaggat ttaccagaaa ttccaagaag
    15421 gaagtgccat gtcagacacc atcataaaat tggatgcggt tcaaaaggtc tattatttgg
    15481 atactgtcga ggtcgaagcg attagagata tatcgcttga aatacgcgcc ggtgattttg
    15541 tgagtattgc ggggccttcg ggttctggaa aaaccacgat tctcaatttg ataggctgtg
    15601 tcgataagcc gacttcaggc actgtcattg tgaatggcca gcgaacgagt gatctcgacg
    15661 atgatagact tacagagctg cgtcaccgct ccataggatt tatctttcag accttcaatc
    15721 ttattcctgt cctcaatatt cgcgagaatg tagagctacc tcttcttctc gacggcttgt
    15781 caggaagcga atccaaggca gaaagagaaa agtgggtgga ttttcttatc gatagcgttg
    15841 gtcttaagga tcgtatgctg cataaacccg ctgagctctc tggaggccag cgtcagcgcg
    15901 tcgcaattgc tcgcgctctt gttatgaagc cagcaattgt actcgcagat gaacccactg
    15961 ccaatctaga ctctgccact ggcgaaagca tcttgcgtct tatgcgcaaa atgaacgaaa
    16021 cttttggcac gacttttatc ttcagcaccc acgatcctga catagtcgaa gaagccgatc
    16081 acattataag gctcaaagat ggcataatcg tagaggatcg cagaatagtt gaagatcagg
    16141 atgataaatt gggttttcgg ccttcggtta gagccactag cccagaagcg actgacacag
    16201 ggcttactgg catagaagcc actggcacag agggcagtca gccatgattt ctgcacgtct
    16261 cgcatggagg aatctcggcc ttcaccgaaa gaaatcccta atcataggtc tcataatggg
    16321 actcggaatt cttattctat ttgttggcaa ctctttgatc gatacgacga tctccggcct
    16381 gcgccgcatg tttgtagaag gatacactgg ggacctgatg gtaaccggtc ctacggaatt
    16441 ctctaccacc gtgctcggcg aaaccgcagg tagcgaagaa gtgttgcctc atatcgctca
    16501 aatcaaaaaa tacgaagatt ttttgaaatc acgccaagat gtagcctcga ttcttccaat
    16561 cttgagtggg caagtatcct ttggaattgg cgaggaagaa ctcggatcag gctattgttt
    16621 tggtgtgaat atctctgatt atcgggcttt ttttcctgat aatgtgagtc ttctgtcggg
    16681 cgagtggcca gaagagagcg atgaaccttg ggttcttctc tcagagccta cctatgcatt
    16741 gcttcagcag agcagcaaca ccgaagtaaa gcccgggaca aagataatca tgactgcgct
    16801 ggccaattca gcgggcactg taatacgcga agtcagcgtg aa
//
LOCUS       DAOSOB010000044        16578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3519_length_16578_cov_75.867397, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000044 DAOSOB010000000
VERSION     DAOSOB010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 16578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16578
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3519_length_16578_cov_75.867397"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1045
                     /locus_tag="PLS88_05885"
     CDS             161..1045
                     /locus_tag="PLS88_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOU06904.1"
                     /translation="MEIRDAVYVYRLISGERLAWHGRYHAHRAGEFEFHYFMEGQGAL
                     LINRSKFVIDANSIYFVQPREFHSILPEAVEKPISYYAILFAPEADNPADREIVALME
                     HLWHSKNRVLRADAKDRFLLEDLYRLSKSNSERSRKAAEHALVSILYRWFDQIEASID
                     FINSKRSKNEHVERALALMSKHVRDKLSSDDLAAMLGLSEEYFIRLFRQHLGMSPFQY
                     FTRLKIEAASGVLVDNRLTVSDVATRFGFENPFHFTKVFKKCTGLPPREYRKIFFEAG
                     KPPATPERPPLAQQASNS"
     gene            1076..2824
                     /locus_tag="PLS88_05890"
     CDS             1076..2824
                     /locus_tag="PLS88_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06905.1"
                     /translation="MNEFKKLLHFARPYRKIALFSFVMLIIMVTCDLAVPRLVGHIID
                     QGIRQKDLNIVLSTSAIMLGISVLSALAAVLNSVYSIRVGEGIARDLRESIFIKIQNF
                     SYGNVDQFSTAKLMVRLTSDTAAVQRLFQMSLRLGSRAPLSMIGSIALMFVTSKTLAL
                     TMMPIFIVGAVVIFFFSAKMEPFFRTVQQRLDRLNTVLQENIAGARLIKAFVRGEYET
                     ERFEIANDNLAQENVRVMQIMASMSPILTMLINIGIILVVWLGGLQSIQGDLTLGQIV
                     AFTNYLMATLHPLISMTQLSNNWANGLASSKRINEVLDAEPEVAELPKASSLPQTIRG
                     EVEFDHVYFHYNGDTAIKVLENVCTRAEAGKVTAILGATGSGKSSLVNLIPRFYDARS
                     GKVNIDSMDVRLLKISSILEHIAIVPQETVLFSGTVRDNIRYGRPDATDEDVIAAAVA
                     AQAHDFIMNMPEHYETHIEERGANLSGGQKQRIAIARALVTKPSILILDDATSAIDVE
                     TETRIHAAIARAAKGITLIMVAQRISTVLNADKIIVLDEGKVVAEGTHRELLRKSPIY
                     KEIYDSQLGTGVRHGL"
     gene            2814..4721
                     /locus_tag="PLS88_05895"
     CDS             2814..4721
                     /locus_tag="PLS88_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06906.1"
                     /translation="MAYETSPSIQDVRTSFQRRAGRPTRYERASDPRKAMKRLFGYLM
                     PFRQALILIVSLVLLQSLLGLVGPYLLGSAIDQSIGGKDINGLARNAVLMLIAYFLSN
                     SFNLIANRKMATISQDALKNLRHDLFVHIQSLPMRFFDTNSAGGLMSRLTNDIDAINQ
                     AVSQNIISLVAGVITMSGILVSMFILNHWLALASLIVIPIMYWFSNFVAKYTRKGFRE
                     LQKELGDLNAVAEETISGFKVIKAFRRNDSAIETFRKKNDSVYKAAVYANSYALLLMP
                     LTGVIGSFFVIVLASLGGWLALRDLVSIGMIATFINYAQNFTQPLRQISNLYNSIQAA
                     LAGAERVFEIVDMAPETVKIPTLEDARANSENATKADDNSMLDASKAPTVFSLEQFQG
                     NVSFKNVVFSYIPGKPVIKNFNVDIPAGLTVALVGPTGAGKTTITNLLTRFYEIDEGQ
                     ILFDGVDIRTIPKEVVRRAIGLVLQDTFLFADSVLENIRFGRLDATDEECIQAAKMAE
                     VDHFIRQLPDGYQTNLSERASNLSQGQRQLLSIARAILADPTILILDEATSSVDTRTE
                     LRIQRALLRLMQGRTSIVIAHRLSTIRDADKIIVIDNGEIVEIGDHQALLAKRGFYHH
                     LYMSQFKGNEI"
     gene            4778..5299
                     /locus_tag="PLS88_05900"
     CDS             4778..5299
                     /locus_tag="PLS88_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOU06907.1"
                     /translation="MNRRDVPLKDFHYDIAQLRDYWFALFAGDFYAGVFNAMTISWGS
                     YGHVWDKMVFQVMVRPSRYTYEFMNRYDSFTLNAFAPKYRKALSILGSNSGRNLDKIA
                     LAGLTPMASRKVAAPSFEEAKLVIECQKIFWQDLDSSNFLDSSIEEHYPSKDYHRIYL
                     GEIVHVSQQIIEP"
     gene            5450..6211
                     /locus_tag="PLS88_05905"
     CDS             5450..6211
                     /locus_tag="PLS88_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06908.1"
                     /translation="MNLLEIKNITLLKEGRPILNNLSMEIWNGHVHAIIGPNGAGKST
                     LANTIMGLSGYRDFEGDIVFNGESLKGLSIDERARKGITLLFQEPARFEGLSVSQFIL
                     AGAKEKSQEIVEEALRQVGLVPENYMKRAVDKTLSGGERKRIELASIYAMKPRLVLMD
                     EPDSGVDIDSVKYIFGIIKDLKQAGSTVILITHSPEVLKRADHAFLICAGTLVDKGEV
                     SRMFDYFSGKCIPCPHKGQPDTDKIISNVSTFALN"
     gene            6222..7166
                     /locus_tag="PLS88_05910"
     CDS             6222..7166
                     /locus_tag="PLS88_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOU06909.1"
                     /translation="MAEQSIIDKDALIAELLASINQHKFAPDVAHIEIHGNEVLNRNL
                     VDGLIVESQSLEDGVSVRIKVKRGVTIKNPVHFCFGLIPENGVQRIVINTVIEEGAHA
                     QFIANCTFPNAINIQHLMNAEIELEKGASLSYFERHVHGPNGGVKIVPVTKVRLGEGA
                     RFSTEFELIKGAAGVIELDYAAEVEKDATVDMKTRIFGRLEDSIHIRESANLLGESST
                     GVLTSHIAIKDKASAIIENEIVANAPYARGHVDCKEIVQNEARARAIPIVQVNSHLAH
                     VTHEAAIGSVDSKQLETLLSRGLTEDEATDLIIEGLLS"
     gene            complement(7258..8295)
                     /locus_tag="PLS88_05915"
     CDS             complement(7258..8295)
                     /locus_tag="PLS88_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06910.1"
                     /translation="MADVILSVKNLKKYFEPHQGFAESIIGGGQKKKIKAVDDVSFDI
                     KRGEIFGLIGESGSGKTTTGKLAMKLLEPTSGQIIFNGEDVTNLDKEKLKDYRRKVQM
                     IFQDPYASMNPRFKIRDVLEEPLIIHKIKATVEERNTMIVKALEEVKLTPPDDFMGRW
                     PHMLSGGQRQRVATARTLILNPMMLVADEPVSMIDLSTRAEILHMMKEVQKKLGLSYL
                     YITHDLSTARYFTDRIAVMYLGRIVEIGRADDIIDNPLHPYTQALIEAVPEPESGKVK
                     IIKELPIYGEIPSPADIPSGCRFHTRCPYAIDACKAESEPELMLDGEEHYHACKRAHE
                     VQEERRKRQAG"
     gene            complement(8326..9291)
                     /locus_tag="PLS88_05920"
     CDS             complement(8326..9291)
                     /locus_tag="PLS88_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06911.1"
                     /translation="MNSLLKIENLQMYYFTSKGAVRAIDGIDLELFMGESIGLVGESG
                     CGKTSLGTSILRMPTPPGRYVEGKIFVDGIDVIPLSEKQVRKDIRWQKISMVFQGAMN
                     CLTPVYTIGHQMMETLVEHQDMARSDAEELMKEYLGYVGLPPEILQRYPHELSGGMKQ
                     RAVIATALFLKPQIVILDEPTTALDVIVQAQIINLLKKLKQQFNLSFLFITHDLALEA
                     EISDRICVMYAGKIVELGTNEQIYGKQGPSHPYTERLLAATPRLFKKVEELHFIPGTP
                     PDLIAPPKGCRFSPRCNKAFDRCFEEEPPLVEIEPGHKAACWLFH"
     gene            complement(9297..10706)
                     /locus_tag="PLS88_05925"
     CDS             complement(9297..10706)
                     /locus_tag="PLS88_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06912.1"
                     /translation="MKFADFKYRLSEFWSEFKKERSGLVGLAILVISILIVIFEPFIL
                     PWKEANTKWRSIDYWQDNSAGAPPSWTNFFSKQKAAVTVRLENPEDEIIEMDGGIKLA
                     SYTFDYDYSADKAPLDVIFHMVGHGDLPVQVAVERPDGQIIELAQRFEQGLSGQDIRI
                     SLDNDGRESVFTFIKSHESEEALEAYSSKSFKTCNIVFNQTREGMATEFKPLKGTYKF
                     IVRALLLVPGYSEIEDPYTVVTGSVSGLLGTDDSKRDIFSGLVAGLKWALIIGLLTSA
                     ISVLIGVMYGIISAYFGGKVDSVMQFIYQIVNSIPILPVLIVVSAIFKPSIYMLITAM
                     VLFFWTGSVMTVRSMALQIKEETYIEAAKALGAKHSRIIFKHMVPLLIPYSFASMALS
                     VPSAIVYESSVSLLGLGDATIVTWGQILHDAMQGAAILKGIWWWVLPPGLLIAIMGMT
                     FAFLGFSMDKILHPKLKTR"
     gene            complement(10703..11743)
                     /locus_tag="PLS88_05930"
     CDS             complement(10703..11743)
                     /locus_tag="PLS88_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06913.1"
                     /translation="MYKWFAVKRVLKGIFTYVIIIFIMSVLFNTVNEQTMRSSIEEQV
                     RAETMRLKNMQPSALQHFQEQRRQELIRLYRLDRPLMERILFRTWNTITFNFGKSTII
                     KSSRGDREVLTIVGEAIPRSLILFTAAMAIEIVVGIILGLKKAQKPGSSLDKSTSLIT
                     MIVYGMPTFWLAMILIMFFVYQLKIFPSGGMHTVPPPQGIMYYIDFLWHMTLPLLTLL
                     LIGFWGIAFVVRNIVLSILQEDYIMAARARGIPENKVLFGHTLRTAAPPLITMSVLSL
                     LASIGGAIIFEGIFSWPGLGNLYWIAVQQNDIPVLMGDLAITVGLYQVGLILLDLTYG
                     FMDPRIKVGGKA"
     gene            complement(11867..14440)
                     /locus_tag="PLS88_05935"
     CDS             complement(11867..14440)
                     /locus_tag="PLS88_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU06914.1"
                     /translation="MKKILCVSFVAMVLLFGATAQVKNGAYADKVIYDVKMDQTIAIK
                     DTAEGKTDVFFTGLDGKTYKGIQPADLERLSTYAVPSGSWSLLLNNIPNKAPYTFQTK
                     DGRTVFNPLAIQEVRYAINWLIDRKKIVDEILLGAGEPMITAQTPGQPGTYRFNLVPA
                     KLGMTVRGDEKKALADIEAALKAAADLPENKGKLVKSGQWWTYNKEPITVKFIIRVDD
                     PTGRLLEGRYIADQIEKAGIKVERLEYDRSKASNLAYSSDPADWQWSMYTEGWGAGAT
                     RAWWDVTISQMYSPYYGYMPGGMTEGFWNYENKEIDRIAQKNINGWFLTADEYWNDNI
                     KVQEMALKEAVRIYVCSQVQYYVANKARFNSRMLYGLGDGLNNWSVRSADVKPNEKGE
                     KILKITQYSARGGLFMSSWDPVGVDGFSDVYSAAIIEACTDPSTFESPNAAKDTPLRV
                     KYDLKNVETKIAAGKEGEPPVGLIPVDKAAVIFNSKTKKWESGVEYKDVTGEGTYDYV
                     KNNDLKSYSKLANQQYIYGKWHSGQPITVADIMYATAFAYEWANKDSEDDKYYDAAYA
                     SQYQAYLPVSKGTVLNKDGSFTTYFDFNWPMDLDRVAATGAVSPKAGNPGRQTLVSWE
                     IYEALAKIVAEGAKSGTQYSFSNDPSMTEVDIINPKCVADIKAKLQDFVAAKYVPDCI
                     KQWITPEQAVARYNAAIKFIDTYGNAYISNGPFFLSKVDFNTNYVELSAFRDYPYKAD
                     YFPKLFRTTITRIDDVKVPATAARNADAKIDIQVSAVTYPDDTAKAADNKAKVTVSLI
                     KADNTETVYTAKYVKAGTFQAVIPAKDLGALKSGSYTIVVQSVFSTEAPAVQATSLVV
                     F"
     gene            14594..16552
                     /locus_tag="PLS88_05940"
     CDS             14594..16552
                     /locus_tag="PLS88_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOU06915.1"
                     /translation="MSIISVSNGHFIDDKGRWLILRGVNLGGSNKVPTAPDGRTHLRE
                     GFYNSADVSFVGRPFPLEEADEHFSRLAGWGQKFLRLVVTWEAIEHVGPGIYDIEYLD
                     YLEGIVEAAARHGISLFIDPHQDVWSRWTGGDGAPMWTLEAVGFDPSKLHESGAALLH
                     QEMGALYPEMQWFSNHLRLGCATMFTLFFAGNDYAPGITTEGRPIQEYLQEHYFEAFA
                     VLAKRIASYDNVVGFGSMNEPGEGFIGINDVSAPRSDMLLPGLAPTPWEAMCAGEGFE
                     TSANRIAIKGLGLRPLERVPLGTKGVRAWKEGEVCVWRRVGLWDIEKDRPVLKQPSWF
                     APKGTYSAETRDGKNKTVQNTHSVFNECYLKPFVERFAEHLAHVSGKPKRFMVFVEGY
                     PQGDMPALRPDFLCVNESHWYDSLTLTMKRWTGFLAYDSEMQKVIIGSHAVRRYFRDA
                     LARIVQHSREFMGNSPTLLGEFGLPFDLNNRKAYRTGNFELHEKVLSLYYDALDANLL
                     SATLWNYTADNTHEHGDGWNREDLSVFCNEDGGGRAVAGFVRPYAYAVSGEILSMQFN
                     RRKGRFILEYSPDRSIEAPSEVFVPMLHFPRGAFVSAFGATFSESDLHIEKGTKSISL
                     PGELSQPLLLKIEAEPDAELCRLVISRL"
BASE COUNT         4426 a         3876 c         3848 g         4428 t
ORIGIN      
        1 catcgccgct gacttcctct agagatgaaa tctgctatcc aaaaaattta cgtaagcatc
       61 aagctaacgt aaaaaactat aaggtcatta tcctaaaaat acaatgcgag atattgacaa
      121 attatatcca ttattgacat tctctctata tggagattgt atggagattc gcgatgctgt
      181 ctatgtatac aggctcataa gtggagagag attggcatgg cacggtaggt atcatgctca
      241 tcgagccgga gagttcgaat tccactattt catggaaggg caaggcgctc tgctcatcaa
      301 tcgttcaaaa ttcgtcatcg atgccaatag catttacttt gtgcagcctc gcgaatttca
      361 ttcaattctt cccgaagccg tggaaaaacc tatcagttat tatgcaattc tattcgcgcc
      421 agaagctgac aatccggcag atagagagat tgtagcgctg atggagcatc tatggcactc
      481 aaagaatcga gttttgcgag cagacgcaaa agatcgattc ctccttgaag atctatatag
      541 gctgtctaaa tccaactctg agcgctctcg aaaagcagcg gagcatgcgc ttgtcagtat
      601 tctatatcgc tggttcgatc aaatcgaagc ctcgattgat ttcatcaatt ccaaaagatc
      661 gaaaaatgag catgtcgaaa gagctctcgc tcttatgtct aagcatgtaa gagataaatt
      721 gagctcagat gacctggcag ctatgctggg tctttccgaa gaatatttca tacgactttt
      781 cagacagcac ttgggcatgt ctccttttca atattttaca aggttgaaaa ttgaagccgc
      841 atcaggtgtg ctcgtggaca atcggcttac ggttagcgat gtcgccacac gctttggatt
      901 cgaaaatcca tttcatttta ccaaggtttt caagaaatgc acgggacttc cacctcgaga
      961 atatcgcaaa attttcttcg aagcaggaaa acctcccgct actccggaga ggcccccgct
     1021 ggcacaacaa gcctcgaata gttaaaaata tagccgctaa aatacggagc ttctaatgaa
     1081 cgaattcaag aaattattgc attttgcgcg cccatatcga aaaattgcgc ttttttcctt
     1141 tgtgatgctt attatcatgg tgacttgcga ccttgcagtg ccaaggctcg tcggccatat
     1201 catagatcaa gggattaggc aaaaagactt aaacatcgtt ctttctacat cggctatcat
     1261 gctaggcata tctgtactgt cagccctggc tgccgtgctc aatagcgtct attcaattcg
     1321 ggtcggggaa ggtattgcac gcgatttgcg cgaatcaatc tttatcaaaa tacagaattt
     1381 ttcatacgga aacgtagacc agttttcgac cgctaagctt atggtccgcc ttacaagcga
     1441 cactgctgct gttcagcgac tttttcagat gtcgcttcga cttggatcga gggcaccgct
     1501 ctcgatgatc gggagtattg ctctcatgtt cgtgaccagc aagacattgg ctcttacgat
     1561 gatgccgatt ttcattgttg gagcggtcgt catcttcttt tttagcgcga agatggagcc
     1621 ttttttcagg accgtccagc aacgtctcga tcgccttaat accgttctgc aggaaaacat
     1681 agcaggggca aggcttatca aggccttcgt gcgaggagaa tatgagacgg aacgcttcga
     1741 aatagccaac gacaacctag cccaagaaaa tgttagagtt atgcagatca tggcatcgat
     1801 gagccccatt ctgaccatgc tgataaatat cggcatcatt ctcgttgtat ggcttggggg
     1861 tttgcagtcg attcagggtg atcttacgct ggggcaaata gttgccttta caaactacct
     1921 catggccacc ctccaccctc ttatttccat gactcagctt tctaataact gggcaaatgg
     1981 tctggcatca tcaaagcgaa tcaatgaggt gctggatgcc gagccagagg ttgcagagct
     2041 gcccaaggcc tcttcccttc cacagactat tcgtggagag gtcgaattcg atcatgtata
     2101 tttccattac aatggagaca cagccatcaa agtgctggag aatgtctgta caagagccga
     2161 agcaggaaag gttactgcta ttctaggcgc gacaggttcg gggaagtcca gccttgtcaa
     2221 cctgatcccg cgcttctacg atgcacgttc aggaaaggtc aatatcgatt ccatggacgt
     2281 tcgactccta aagatttcat cgattctcga acacatagcc atagttcctc aggagaccgt
     2341 gctcttttct ggtacagtcc gcgacaacat tcgatatgga aggcccgatg caacagatga
     2401 agatgtcatt gctgccgcag ttgcggccca agctcacgac ttcatcatga atatgccaga
     2461 acattatgag acacatatcg aagaacgcgg agcgaatctt tcaggcgggc aaaaacagcg
     2521 tatagctata gctcgtgccc ttgtcaccaa accttcgatt ctaattctcg acgacgcaac
     2581 gagcgccata gatgtggaaa cagaaacaag aattcatgcg gccatagcaa gagctgccaa
     2641 gggaataacc cttatcatgg tggctcaacg tatcagcaca gtgcttaatg cagacaagat
     2701 aatcgtgctc gatgaaggaa aggttgtcgc tgaaggcaca cacagagagc tcctaaggaa
     2761 aagcccaata tacaaagaaa tatacgattc ccagttgggt acgggggtgc gccatggcct
     2821 atgagacaag cccttcgatt caggatgtgc gcaccagctt ccaaaggcgt gcaggtaggc
     2881 ccacaagata tgaaagagcc tcggaccctc gcaaggcgat gaaaaggctc ttcggatatc
     2941 taatgccatt cagacaggca ctgattttga ttgtgtccct tgtgcttctg caatcgctcc
     3001 ttggattggt aggcccttat ttgctcggca gcgcaataga ccaaagtatc ggaggcaaag
     3061 acataaatgg cttagctcgc aatgctgttc ttatgctcat tgcttatttt cttagcaata
     3121 gctttaatct gatcgcaaat aggaaaatgg cgactatatc ccaggatgct ctcaagaact
     3181 tacgccacga tctattcgtg catatccaat cgctccctat gcgctttttc gatactaatt
     3241 ccgcaggcgg gctcatgagc agactaacga atgacatcga cgctatcaat caggccgtat
     3301 ctcagaatat catctcgctt gttgctggtg ttattacaat gtctggtatt ctagtgagca
     3361 tgtttattct aaaccattgg cttgccctag ccagcctcat cgttatccca ataatgtatt
     3421 ggttcagcaa ttttgtagca aaatacactc gcaaaggatt cagagaattg caaaaagagc
     3481 tgggcgatct caatgccgtg gctgaggaga ctatctctgg tttcaaggta atcaaagcct
     3541 tccgtcgcaa tgattcagca atcgaaacct tcaggaagaa aaatgattcg gtatataagg
     3601 cagctgtcta tgcaaattcc tatgcattgc ttctaatgcc tcttacaggg gtaatcggca
     3661 gtttttttgt catagtgctg gctagtcttg gcggttggct agctcttaga gatcttgtca
     3721 gcattggcat gatcgccaca ttcataaact acgcacagaa ttttactcag cctttaaggc
     3781 agatatcgaa tctgtacaac tcgatccaag cggcgctagc tggagccgag cgagtcttcg
     3841 agatagtcga tatggcaccg gaaactgtta agataccaac attagaggat gcaagagcga
     3901 actctgaaaa tgcaacaaaa gccgacgata attctatgct cgatgcttcc aaagctccta
     3961 cggtctttag cctcgaacaa tttcagggca atgtaagctt caagaatgta gtattcagct
     4021 atattcctgg taagcccgta atcaagaatt tcaacgtaga tatccctgcg gggcttacgg
     4081 tagcgttagt tggacctacg ggagccggta agactacgat tactaacctt ctcacgcgtt
     4141 tctacgagat cgatgaagga cagattctat tcgatggtgt ggatatccgc actattccca
     4201 aagaagtagt ccggcgcgcc ataggtcttg ttctgcagga tacttttctc tttgcggatt
     4261 ctgtgctaga gaatattcgc tttggtaggc ttgatgcaac agatgaggaa tgtatccaag
     4321 ccgccaagat ggcggaagtc gatcacttta ttcgacagct ccccgatggc taccagacaa
     4381 atctttcgga gcgcgcgagc aatttgagcc aaggccaacg ccagctttta tcaattgcgc
     4441 gggctatcct tgcagatccg acaatactca tcctggacga agccactagt agcgtcgaca
     4501 cgcgcacaga acttcgcatc caaagagctc ttttgcgact catgcaggga agaacgagca
     4561 tagttattgc tcatagactt agcaccattc gcgatgccga caagattatc gtaatcgaca
     4621 acggcgaaat tgtagaaata ggagatcatc aagcgctcct cgcaaagcgg ggcttttatc
     4681 atcatcttta catgagtcaa ttcaaaggaa atgaaatata atcgaagaca gattatttag
     4741 ttctattcaa gcatccataa agcatgggag gaaaaacatg aatcgaaggg atgtaccgct
     4801 aaaagatttt cactacgata tagctcagct gcgcgattat tggtttgcgt tatttgcggg
     4861 tgatttttat gcaggggtct tcaatgcgat gaccatttcc tggggcagct atggacatgt
     4921 atgggataag atggtgtttc aggttatggt acgtccctca aggtatacct atgaattcat
     4981 gaaccgatat gatagcttta ctctcaatgc ttttgctccg aaatatagaa aagctctctc
     5041 cattttaggt tctaattcag gccgaaatct ggataagata gcattggctg gccttactcc
     5101 tatggcttcg cgcaaggttg ccgctccaag cttcgaggag gcaaaacttg taatcgaatg
     5161 ccaaaaaatc ttttggcagg acctcgacag ctcaaatttc ttagatagct cgatagaaga
     5221 gcactacccc agcaaggatt accatcgaat ttatctaggc gagattgtcc atgttagtca
     5281 gcaaataatc gaaccctaag gcattcggct ccatttattt tctcttttgg cgcatacttg
     5341 attttctttt aaaaaacagg tattttctca aaggaaaatc ctatatcagc gatatgaatt
     5401 ttatccagag tacgacaagt ctcagcctaa aatgcaggat ggcaagataa tgaatcttct
     5461 cgaaatcaag aatatcacac ttctcaaaga gggcagaccc attttgaaca atctcagcat
     5521 ggagatctgg aatggacatg ttcatgcgat aattggtcct aatggagcag gcaagtctac
     5581 actagctaat acaataatgg gcctatctgg ctaccgcgat tttgaaggcg acatcgtttt
     5641 caatggcgag tcccttaaag gtctttcgat tgatgagcga gcgcgaaaag gaatcacatt
     5701 gcttttccaa gagcctgcac gcttcgaggg gctttccgta agtcagttca tacttgcggg
     5761 agccaaagag aaatctcaag aaatcgtcga ggaagcactt cgacaggtcg ggcttgtacc
     5821 agaaaactat atgaaacgcg ctgtggataa aacactctct ggcggtgaac gaaagcgtat
     5881 agagcttgct tcgatctatg caatgaagcc tcgccttgtc cttatggatg agccagattc
     5941 aggcgtggat atagattcag tgaaatatat cttcggcatt ataaaagatc taaagcaagc
     6001 aggctccact gtcatcctca tcacccatag ccctgaagtc ctaaagcgtg cagatcatgc
     6061 atttctcatc tgtgctggaa ctcttgtcga caagggcgaa gtatcgcgaa tgttcgacta
     6121 cttcagcggc aagtgtatcc cctgccccca caaaggccag cccgatacag acaaaataat
     6181 ctccaatgtt tccacatttg ctttgaattg agaggaaaac catggccgaa caatcaataa
     6241 tcgataaaga cgcgcttatc gcggagctct tggcgtcgat caatcaacat aaattcgccc
     6301 cagatgttgc acatatagaa atccatggca atgaggtctt gaatcgaaat ctcgtcgatg
     6361 gccttatcgt agaatctcaa tccttggagg atggagtaag cgttagaatc aaagtcaaac
     6421 gaggtgttac aattaagaat ccagtacact tttgcttcgg tcttattcct gaaaatggag
     6481 tgcagcgaat cgttataaat accgtaatcg aagaaggcgc ccacgctcaa ttcatagcaa
     6541 actgtacctt cccaaatgca atcaacatcc aacatctcat gaatgcagag atcgagcttg
     6601 aaaagggtgc atctctatct tacttcgagc ggcatgtcca tgggcccaat ggcggtgtca
     6661 aaatcgtgcc ggttacaaaa gtaaggctag gcgaaggagc acgattttcc actgagttcg
     6721 agctcattaa aggagcggcc ggtgtcatcg aactcgatta tgccgcagaa gtggaaaaag
     6781 acgccacggt ggacatgaag actcgtatct tcggtcgact cgaagatagt atacacatca
     6841 gggaatccgc caatcttctt ggcgaatcct cgacaggtgt actgacaagt catatcgcca
     6901 tcaaagataa agcttctgcg atcatcgaaa acgaaatcgt cgccaatgcc ccttatgcac
     6961 gaggccatgt cgattgcaag gaaatagtcc aaaacgaagc tcgagcccgt gctattccta
     7021 tagttcaagt gaacagccat cttgcccatg tcacacatga agcggcgatt ggctcggtcg
     7081 actcaaaaca gctagagact ctgctaagcc gtgggctcac cgaagacgag gctaccgatt
     7141 tgataataga agggctcttg agctaatttc ccgctctagc tctaagcgct cgaagcaata
     7201 aatcatacgg ccatgcgagg cgaatcctaa tacacaatag aatcgcattg ctttgctcta
     7261 ccctgcttgt ctcttccttc tctcttcctg aacctcgtgg gctcgtttac aagcgtgata
     7321 atgctcctct ccatccagca tcaactcggg ctcagattca gccttgcaag catcgatagc
     7381 ataggggcag cgagtatgga agcggcagcc acttggaatg tccgcggggc tcggaatctc
     7441 tccatagatt ggcagctctt taataatttt aacctttcct gattctggtt ctggtacagc
     7501 ctcaataaga gcttgagtat atgggtgtaa agggttatcg atgatatcat cggcgcgacc
     7561 aatttccact atgcgaccca agtacattac cgcgatgcga tcggtaaaat agcgcgccgt
     7621 cgaaagatca tgcgttatat agagataaga caggccaagt tttttttgta cctccttcat
     7681 catgtgaagg atctcggcac gcgtggaaag gtctatcatg gagaccggtt catctgctac
     7741 cagcatcatt gggttgagta tcagagttct tgccgttgcg actcgctgac gctgaccgcc
     7801 ggaaagcatg tgcggccatc gacccatgaa atcatcgggt ggtgtgagct tgacctcttc
     7861 aagagctttg acgatcatgg tgttgcgctc ttcgacggtc gccttgatct tatggatgat
     7921 gagaggctcc tccagcacat ccctgatttt gaagcgcgga ttcatcgaag cgtaggggtc
     7981 ctgaaagatc atctgaactt ttcgacggta atctttgagc ttttctttgt cgaggttcgt
     8041 cacatcttcg ccgttgaaaa ttatttggcc tgaagttggt tcgagaagtt tcatagccaa
     8101 cttccccgtc gtagtctttc cagaacccga ttcgcctatt aatccaaata tctcgccgcg
     8161 ctttatatca aaagaaacat catcgactgc cttgattttc ttcttctgcc caccacctat
     8221 gatcgactcg gcaaatcctt gatgtggttc gaaatatttc ttgaggtttt tgacactcag
     8281 tattacatcc gccatgtatt ttctcctctc tggctttccg gtttctcaat gaaatagcca
     8341 acatgcggcc ttatggcctg gctctatttc aaccaagggt ggctcttcct cgaaacagcg
     8401 atcgaaagcc ttattgcagc gaggactgaa tcggcagcct tttggtggcg caataagatc
     8461 gggcggcgtg ccaggaataa aatgaagctc ctctactttc ttgaaaagcc ttggagtagc
     8521 cgccagaagt cgctctgtgt agggatgaga tggtccctgt ttgccataga tttgctcatt
     8581 cgtgcctaat tcgacgatct tgccagcgta cattacacag atgcgatccg atatctcagc
     8641 ttcaagcgca aggtcatgtg taataaacaa aaacgagagg ttgaattgct gcttgagttt
     8701 tttgagcaga ttgatgatct gtgcctgaac aatgacatct aatgctgttg taggttcatc
     8761 gagaatgacg atctgaggtt tgagaaagag agcggtggcg atgaccgctc gctgcttcat
     8821 gcctcccgaa agctcgtgtg gatagcgctg caatatctct ggaggcaagc ccacataccc
     8881 taggtattcc ttcattaact cttcggcatc ggagcgagcc atatcctgat gttcaaccag
     8941 agtctccatc atttggtggc ctattgtata aacaggcgtg agacagttca ttgctccctg
     9001 gaagaccatc gatatttttt gccagcgtat gtctttacga acttgcttct cggaaagtgg
     9061 aatcacatct attccgtcta cgaatatctt accttccaca taacgacccg gtggagttgg
     9121 catgcgcaat atagaagtgc caaggcttgt cttaccacag cccgactcgc ccacaagtcc
     9181 tatgctctcg cccataaaga gttctaaatc tatcccatcg atggcgcgca cagcgccttt
     9241 cgaagtaaag taatacatct gaaggttttc aattttcagc aagctgttca tcgaggttac
     9301 cttgtcttta gttttggatg gagtatcttg tccatcgaga agcctaggaa tgcaaaggtc
     9361 attcccataa tagctattag aagtcctggc ggcagcaccc accaccagat acctttgagg
     9421 attgctgccc cttgcattgc atcgtgtaga atctgacccc aggtgacaat agttgcgtct
     9481 ccgaggccca atagactcac cgaagattca tagactatcg cactcggtac agacaaggcc
     9541 atggatgcaa agctatatgg aatcaaaagc ggcaccatat gcttgaagat gattctagaa
     9601 tgcttagcac cgagtgcttt ggcagcctcg atatatgtct cctccttgat ctgaagagcc
     9661 atggatctga ctgtcatcac ggagccagtc cagaaaaaga gcaccattgc agtaatcaac
     9721 atatatatac tcggtttgaa aatagctgat accacaataa gaactggcag aattggtatt
     9781 gaattcacaa tctgatatat gaattgcatt accgaatcta ccttacctcc gaagtaagcc
     9841 gagatgatgc catacatgac tccaatcaac actgagatcg cagaagtcaa aagcccaatt
     9901 ataagcgccc acttgagacc agccacaagc ccagagaaga tgtcgcgctt tgaatcatcg
     9961 gttcctaata atcctgaaac agaacctgtc acgacagtat atggatcttc gatctcagaa
    10021 tatccaggaa caagcagcaa ggctcgcaca ataaacttat aggttccctt aagaggcttg
    10081 aattcggttg ccatgccttc tctcgtctga ttgaacacta tgttgcaggt cttgaaactc
    10141 ttgctggaat acgcttcgag agcttcttcg ctttcatgac ttttgatgaa agtgaacacc
    10201 gattctctgc catcattgtc caaggagatt cggatatctt gaccacttag gccttgctcg
    10261 aaacgctggg caagctcgat gatctggcca tcgggccgtt ctaccgcaac ttgaactggt
    10321 agatccccat gtcctaccat gtggaatatg acatcgaggg gagctttatc tgcactatag
    10381 tcataatcga aggtatagct ggcaagtttg atgcctccat ccatttcgat tatttcatcc
    10441 tcgggatttt ctagacgaac ggtgactgca gccttctgct ttgagaaaaa gttagtccat
    10501 gaaggaggcg cacctgccga gttatcctgc cagtagtcta tgctccgcca tttggtattc
    10561 gcttctttcc aaggcagaat gaaaggctcg aagattacta tgagtatcga aataaccaaa
    10621 atcgcaagcc caacaaggcc agaacgttct tttttaaatt cggaccagaa ctctgaaagt
    10681 cggtacttga aatcagcaaa tttcatgcct tacctcccac cttgatgcgc ggatccataa
    10741 agccataggt gagatccagc aatataagcc ctacctggta taagcccact gttatagcaa
    10801 ggtctcccat tagaacgggg atatcattct gctgcaccgc tatccagtat agattcccca
    10861 atcctggcca ggaaaaaatg ccttcgaaga tgattgctcc tccaatcgag gcaagcaaag
    10921 acaacactga cattgtgatg aggggaggcg ctgcggtgcg cagggtgtgc ccaaaaagca
    10981 ccttattttc ggggatccct cgggcacgag ctgccataat gtaatcctcc tgaagaatac
    11041 tcagcacgat attcctaacc acaaatgcta tcccccagaa cccaatgaga agcaaggtca
    11101 aaagcggcag agtcatgtgc cacaaaaaat ctatataata cattatccct tgtgggggtg
    11161 gcacagtgtg catgcccccc gaaggaaata tcttcagttg atagacaaaa aacatgataa
    11221 gaatcatcgc aagccagaat gtcggcatgc catagacgat catggtgata aggctcgtac
    11281 ttttatcgag cgaactacca ggtttctgag ctttcttaag tccaagaata ataccgacca
    11341 ctatttcaat agccatagcc gctgtaaaga gtattagcga gcggggaatc gcttctccta
    11401 ctatggtcaa cacctctcta tcgccacgcg aagatttaat gatagtcgat tttccaaaat
    11461 tgaaggtgat ggtgttccaa gttcgaaaaa gtattcgctc cattagcggc cgatccagcc
    11521 tatagagcct aattagctct tggcgtcgct gctcttgaaa atgctggaga gcagacggct
    11581 gcatgttttt gaggcgcatg gtctcagccc gtacctgctc ttcgatgctt gagcgcattg
    11641 tctgttcatt gaccgtgttg aaaaggacag acattataaa aatgattata acataagtaa
    11701 aaatgccttt tagcactcgc ttgacggcaa accacttata cataaaacca cctgtttctt
    11761 tgtgattcct tgagaaaatt gcacttcgat tattacatta aaaaagcatt ttataaaaga
    11821 aggagggtgc cgcctcttag agacagcacc cctcccttga ttcaaatcag aagactacaa
    11881 gcgaagtagc ctggactgct ggtgcttctg tactgaaaac agactgcact actatagtgt
    11941 aggagcctga tttgagagca cccaggtctt tagcaggaat cacagcctgg aaagtacctg
    12001 ctttgacata cttagcggtg tacaccgtct cggtattatc tgcctttatc aaagaaaccg
    12061 ttacctttgc cttgttatct gccgctttgg ccgtatcgtc tggataggtc accgcagaaa
    12121 cttggatgtc tatcttcgca tcggcgttgc gcgccgcagt tgccggtact ttcacatcat
    12181 cgatgcgggt aatggtcgtg cggaacaatt tcgggaaata atccgctttg tatggataat
    12241 ccctgaaagc agaaagctca acatagttgg tattgaaatc taccttgctc aagaagaatg
    12301 gaccattgga tatgtaggca ttgccatagg tatcgatgaa ttttatggct gcattatagc
    12361 gagcaacagc ctgctccggg gtaatccact gcttgatgca gtcaggaaca tacttggcag
    12421 cgacgaagtc ctgcagtttt gccttgatat ccgcgacaca cttgggattg atgatatcga
    12481 cttccgtcat tgaaggatca ttcgagaaag aatactgtgt tccagacttt gcgccctctg
    12541 cgacgatctt ggctaaagct tcgtagatct cccatgagac tagagtttgg cgtccggggt
    12601 taccggcctt ggggcttaca gcaccggttg ctgctacacg gtcaagatcc atcggccagt
    12661 tgaaatcgaa ataggttgta aagctgccat ccttattgag cacagtacct ttggaaaccg
    12721 gcaaataggc ctgatattgt gacgcataag ctgcatcgta gtacttgtcg tcctcgctat
    12781 ccttgttagc ccactcgtag gcaaaggcag tcgcatacat aatatcagcg actgttatgg
    12841 gctgcccgct gtgccatttg ccataaatgt actgctggtt agcaagcttg ctgtaggatt
    12901 tcagatcgtt gtttttaacg taatcgtatg ttccttcgcc tgttacatcc ttatattcga
    12961 ctccactctc ccacttcttt gtcttggaat tgaagattac tgcggccttg tcgacaggaa
    13021 tcaggccaac ggggggctcg ccttctttgc cggcggcaat cttggtctcg acattcttga
    13081 gatcgtattt tacgcgaaga ggcgtatctt tggccgcatt aggagactcg aaggtcgagg
    13141 gatctgtaca agcttcgatg atggctgcgg agtagacatc cgagaaacca tcgacaccca
    13201 ctgggtccca tgagctcatg aagagtccgc cacgagccga gtactgcgtg atctttagaa
    13261 tcttttcgcc tttttcgtta ggctttacat ccgcagatcg caccgaccag ttgtttagac
    13321 catcgcctag gccatagagc atacgcgaat tgaaacgagc tttattggcc acgtagtatt
    13381 ggacctggct gcatacatag atacgcacgg cttccttcaa agccatttct tgtaccttga
    13441 tgttatcatt ccaatactca tcggctgtaa ggaaccatcc atttatgttc ttctgagcaa
    13501 tacgatcgat ttctttattc tcgtagttcc agaagccttc ggtcattccg cccggcatat
    13561 atccatagta gggtgaatac atctggctaa tggttacatc ccaccacgcg cgggtagctc
    13621 cggctcccca accctcggta tacatagacc actgccagtc tgctggatcg ctgctatatg
    13681 caaggttgct agcctttgag cggtcatatt cgaggcgctc taccttgatt ccagctttct
    13741 ctatttggtc agcgatatat cgaccttcga gaagtcgacc tgttggatcg tcgacacgga
    13801 ttataaattt gacggtgatg ggctccttat tataggtcca ccactggccg gactttacga
    13861 gcttaccttt gttttcaggc agatcggctg ctgctttcaa tgcggcctcg atatctgcca
    13921 gggctttttt ctcatcgccg cgcaccgtca tgcctagctt ggcaggcacg agattgaagc
    13981 gataggttcc aggctgtccg ggtgtctggg ctgtgatcat tggctctcca gcaccaagca
    14041 gaatctcatc cacgatcttt ttgcggtcga tgagccaatt gattgcgtat cggacttcct
    14101 gaatcgccag tgggttgaag actgtccgac cgtctttggt ctggaatgta tagggcgctt
    14161 tgtttggaat gttgttcagg agaagagacc aacttccgga tggcactgcg taggtggaaa
    14221 gtctttccag atcagccggt tggatgcctt tataggtctt tccgtcaaga cccgtaaaga
    14281 aaacatcagt tttcccttcg gcggtatctt tgatggcaat ggtctgatcc attttcacat
    14341 cgtagataac cttgtccgca taggcgccgt tcttgacctg ggctgttgcg ccaaacagca
    14401 gcaccatggc tacgaacgat acacagagaa tctttttcat gcatacctcc tttttctcag
    14461 gtttgcttgc ttccattgta gaacgtgcat tttgagttgt aaagagaaaa atcatgatta
    14521 gtagcttgta gtgccccaat aataagcgta ttctaatatt gattatacag ataagactga
    14581 ggggaaaggc ctaatgtcaa taataagcgt atcgaatgga cattttattg acgataaagg
    14641 gcgctggctt atcctgcgcg gagtgaacct cgggggaagc aataaagtgc caacagcgcc
    14701 ggatgggcga actcatctta gggaaggatt ctataatagc gcggatgtct cgtttgtggg
    14761 caggcctttt ccgcttgaag aagcagacga gcatttttca agacttgcag gctggggcca
    14821 aaagtttctt cgtctagtgg taacctggga agccatcgag catgttggac caggtatcta
    14881 tgatattgaa tatctagact atctggaagg tatagtggaa gctgctgctc gacatggaat
    14941 atccctcttc atagatccgc atcaggatgt atggtcgcgt tggacaggag gagatggagc
    15001 tccaatgtgg acgctagaag ccgtgggctt cgatccatcc aagcttcatg agagcggcgc
    15061 tgcacttctt catcaagaga tgggagcgct ataccccgaa atgcaatggt tttccaatca
    15121 tctacggctt ggctgcgcta cgatgtttac cctgttcttt gcaggtaatg attatgcgcc
    15181 aggtattact actgaaggaa ggccaatcca ggaatatctt caggaacatt atttcgaagc
    15241 ttttgctgtg ctcgccaagc gaattgcttc ttatgataat gtggttggat ttggttccat
    15301 gaatgagcct ggagaaggct ttataggaat taacgatgtc agtgctccgc ggagcgatat
    15361 gcttctacct gggctagcac cgactccctg ggaagcgatg tgtgctggag aagggtttga
    15421 aacgagcgcc aatagaatag ccataaaggg cctaggttta cgcccactgg aaagagtacc
    15481 tttaggcaca aagggcgtcc gcgcatggaa ggaaggagaa gtctgtgtat ggcgtagagt
    15541 tggtttatgg gatatcgaaa aggatagacc agtattgaaa cagcccagtt ggtttgctcc
    15601 taaaggaaca tatagtgcgg aaaccagaga tgggaaaaac aagactgtac aaaatactca
    15661 ttctgttttc aatgaatgtt atctgaagcc atttgtcgag cgatttgcgg agcacttagc
    15721 tcatgtatcc ggcaaaccaa agcgctttat ggtattcgtg gaaggttatc cacaaggcga
    15781 tatgcccgcg ctccgtcctg acttcctctg tgtcaacgaa agccattggt atgactcatt
    15841 gactctcaca atgaagcgct ggactggttt tcttgcatat gattccgaaa tgcaaaaggt
    15901 cattattggc tcgcatgcgg taagacgcta ttttcgagat gcattggcgc ggattgtgca
    15961 gcattctagg gaattcatgg gcaactctcc tactcttctc ggcgaattcg gccttccatt
    16021 cgatctaaat aatcgaaaag catatcgaac tggaaacttc gaactgcatg aaaaggtgct
    16081 ttctctgtat tatgatgccc tagacgcaaa tctcttaagc gcaacgctgt ggaactatac
    16141 agctgacaac actcatgagc atggcgatgg ctggaacaga gaagatctct cggtcttttg
    16201 caatgaggat ggtggtggaa gagcggttgc aggttttgtg agaccttatg catatgcggt
    16261 ctctggagag attctatcga tgcaattcaa ccgaagaaag ggacgtttta tactagaata
    16321 tagtccagac cgttccattg aggcgccttc tgaggtcttt gttccgatgc tgcattttcc
    16381 tagaggagcg tttgtatctg catttggagc cacattctcc gagtctgatc tgcatatcga
    16441 aaaaggcaca aaaagcatta gcttgcctgg ggaactctct cagcctctct tgctgaaaat
    16501 cgaagctgag ccggatgctg aattgtgccg cttggtaatc tcgcggcttt gatagaaaaa
    16561 tccagagcaa tctttcga
//
LOCUS       DAOSOB010000045        16193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3623_length_16193_cov_77.035630, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000045 DAOSOB010000000
VERSION     DAOSOB010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 16193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16193
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3623_length_16193_cov_77.035630"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLS88_05945"
     CDS             <1..732
                     /locus_tag="PLS88_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOU06916.1"
                     /translation="AAFKASIPVLLGYITLGIAFGLTLVAAGLPWWLAPFMALVIYAG
                     AAQFMAVGLIQSGAGLLEIALLTLIMNARHMVYGLSLLEPFSKAGRWKPYLIFGLTDE
                     TYGLLTTIEPPPDLDKTKFYAAITVLNQVYWFVGCMVGSLVGSAFLFNTTGLDFALTA
                     LFIVLLVDQVRAVKKPEPYLAALLGCVLALFISPKNFLLVALVISTGALALLRERIEG
                     RPRGTASQGVSEKSKQDSEEKGDRP"
     gene            729..1043
                     /locus_tag="PLS88_05950"
     CDS             729..1043
                     /locus_tag="PLS88_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOU06917.1"
                     /translation="MTYFVLVCLVMGLVTYFTRALPFLFFSVRKPPELLDFLQKYLPA
                     VVMAILVFSMYKDIDFRAAPYGIPALVAGAITALLHLWKRNVLLSIGAGTAAYIIFIR
                     II"
     gene            complement(1144..4416)
                     /gene="carB"
                     /locus_tag="PLS88_05955"
     CDS             complement(1144..4416)
                     /gene="carB"
                     /locus_tag="PLS88_05955"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOU06918.1"
                     /translation="MMKKVLVLGSGGLKIGQAGEFDYSGSQALKALREENIFTILINP
                     NIATNQTSEGVADATYFLPLTPEFVAQVIEKERPDGILLSFGGQTALNCGLALHKAGI
                     LYKYAVDVLGTPIKAIELTEDRELFANHLRQLGLKTPESKAATNIEEALVCGGTIGYP
                     VMLRAGFALGGAGSGLCKNEKELRARAEEALALSPQVLIEEWLGGWKEIEYEIVRDKA
                     DNCIAICNMENIDPLGIHTGESIVVAPSQTLTDREYHDLRSIALKLVRSLGIIGECNI
                     QYALDPHSGDYRIIEVNARLSRSSALASKATGYPLAYVAAKLALGFCLTDLRNKITGV
                     TTACFEPALDYCVIKMPRWDLAKFKEVDKHIGSEMKSVGEVMAIGRSFEEALQKAIRM
                     TSASQFGLQSDGNQTAIASNRLRASLSKPTDKRLFAVYQALEEGWSVQKINRLTGIDP
                     WFIGKIDSIRQCEFEMRKVSQTARQARVASSAIKLDDKIATILVRAKRLGFSDAGIAH
                     IFGIHELEVRHWRQILGLRPSVRQIDTLAAEYPAQTNYLYVTYAHSFASKSLSRNSVI
                     APSDDVKPSHGSILVIGSGPYHIGSSVEFDWCAVSAVETCRTLGQSTIMVNCNPETVS
                     TDYDICDRLYFEELNFERIMDIYEFEEPSGILLSMGGQIANNLALPLFDAGALILGTS
                     PQNIDKAEDRHKFSSLLDELGIEQPAWSELTTDEEAIAFARSVGYPVLVRPSYVLSGA
                     AMNVAWDDKSLATFLGLAVDISQEHPVVISKFIEHAKEIEIDAVAKHGKLLYHAITEH
                     LENAGVHSGDATVVFPAQRLYVETVRKIIRITEKIAIALNITGPFNIQFVAKQNRVMV
                     IECNLRASRSFPFCSKISRINMIDLATRSMLGQAVQAPPNALYDQPWVGVKAAQFSFA
                     RLHGADPVLGVEMTSTGEVGCIGSELDDAFMKAMVSVGYSPRVKKLLLSTGPLEDKLD
                     FLESAQKLVEMDCTLYASHGTAQFLKKYGIEVQTLHWPLENAEPNILTLMRSHVFDLV
                     INIPKNNRKHELQNDYLIRRMAIDLNIPLFTNIKTARQYIESLAYSKNHGIEIKPWEE
                     YR"
     gene            complement(4413..5600)
                     /gene="carA"
                     /locus_tag="PLS88_05960"
     CDS             complement(4413..5600)
                     /gene="carA"
                     /locus_tag="PLS88_05960"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOU06919.1"
                     /translation="MPVHFKSACLVLEDGSIFEGYSIGADTSEEGEVVFTTGMVGYNQ
                     SLTDPSYCGQILVFAYPLIGNHGVPRLRTNALGIPIDFESDKIQVGAVVVAEASSEPS
                     HYSAERSFSRWLKEENIPGIAGIDTRRLIRVLREEGTMYGKVLVEGTPQPIRDEIKIN
                     NPVKLVSPSSLKRYRQEDAQSKDSSPKNELKKQDSIVNSLEISERPTIALIDCGAKAS
                     IMRILLDAGAEVISMPWDYPLNNIEYDGLFLSNGPGDPKACTKTIAHLRHALGETRPI
                     FGICLGTQLLALAAGADTYKLKYGHRGQNQPAIDTRSGRCYITSQNHGYAVLEDSLPP
                     GWEPWFLNGNDGTIEGIRASNAPFRAVQFHPEGCPGPRDTQFLIEEFLHEVAEAKRQK
                     RQA"
     gene            complement(5741..6697)
                     /locus_tag="PLS88_05965"
     CDS             complement(5741..6697)
                     /locus_tag="PLS88_05965"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOU06920.1"
                     /translation="MLNALQSVFSIIIMIAIGFGLSKARWFEGGGNSLISRLVVNVAL
                     PAYMISNLTGGYDKPTLLSMLPGLVIPFGVMIASYLIAIMVGKILKVKEDRKGTFRSM
                     FALSNSIFIGLPVNLLLFGDKSLPYALLYYIANTTLFWTIGVYGISMDGAVRNRSPMP
                     SLVSLSGLKRILSPPLIGFLIAIFFILVGITLPKSIMDTCKYLGNMTTPLSMLFIGIV
                     IARVDWKKLKFEKDFIAVLAGRFLITPTLMFMVVRSLDMPLLMKEVFVMQASMPAMTQ
                     TPILAETYKSDSEYAAIGTSLTTVASMITIPLYMSLMAVIFD"
     gene            complement(6763..8169)
                     /locus_tag="PLS88_05970"
     CDS             complement(6763..8169)
                     /locus_tag="PLS88_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU06921.1"
                     /translation="MEDVQHESVYVAPAHDAARIEVFRSASIPSAVANLAIPTVISQL
                     VTMIYNVTDTFFVGQLGDPRKVAAVSLVMPAYTMLTAIANLFGVGGSSVISRFLGSGQ
                     PAKAKQTSSFCLISAIFVAAIFSILAFIFQRPLLLLLGANHETYDFASNYIIWVLVIG
                     GIPTVMGMLFGHLVRSEGGARQASIGMSFGGILNIILDPIFIFTLGLEVKGAAIATMI
                     SNCAAVCYFLVYLAHKKGKTIISFVPRKLDQEFSIAAKVVSVGLPASLQNILSLTSNT
                     VLNNLTAKYGSAALAAIGIAKKIDMLPMNVTMGISQGVLPFIGYNYGAKRYDRVHEVN
                     KFTRIVAVCFSLFCILIFELFAEPIVRLFINDPETVRLGASFLRILCLMTPMMAISYL
                     ITTMFQATGQGKRALTISVFRKTTIDVPLMFLMDRLIPLYGLMTVQPIVDTLSVGLAF
                     ALYRDFSKKLDAQTDQEK"
     gene            complement(8283..9641)
                     /locus_tag="PLS88_05975"
     CDS             complement(8283..9641)
                     /locus_tag="PLS88_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOU06922.1"
                     /translation="MKIVLIGAGSAQFGFGTLGDIFSSSLLKGSHIALVDIDGETLSR
                     VYKAAQTFVEAHCLDFTLSAHTVRREALKGADFVVISIEVGNRFELWDQDWMIPLQYG
                     IPQVYGENGGAGGLFHALRIVPPILDICQDCTELCPNATIFNFSNPMTSITTTVLRKF
                     PQLKFIGLCHEIASLARYIPPMLGVPLDKLELRAAGLNHFSVLVSARYRDTGDDAYPE
                     ILAKAPDFFERMIGYSDILEYIQETGNIPKTEGETDLPNIGRTVSSRHWADRRLFRQI
                     LERFHLLPITSDSHIGEYIPWAHDVADNKGILDFYLLYRSSLSMLEPKIELKVKERLV
                     PIIEGILQDSGYEEAAVNIMNNGLIPSLPQNVTVEVPALVHRKGLEGVAFPNYPRAFA
                     ALLRNYTGVYDLTAEAILHKSKDLVIQALLVNPVVPICERVPELVDVMIARQAQWLGY
                     LR"
     gene            complement(9820..10677)
                     /gene="uppP"
                     /locus_tag="PLS88_05980"
     CDS             complement(9820..10677)
                     /gene="uppP"
                     /locus_tag="PLS88_05980"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HOU06923.1"
                     /translation="MLIIESIVLGIIQGLAEFIPISSSAHLIIVPWLFGWDNPALTSL
                     TFDVALHLGTLLAVIVFFAPDWARLISAWFKSVIQFKIGDDPDRRMAWYILLACIPGG
                     ISGLLLESKINEAFHSDPIPRSSMLFMAGSIALLALLLWTADKYARHQRNFSQIKARD
                     ALCIGLAQAFAVIPGVSRSGSTITAGLALGLEREAAARFSFLLSAPIIAGAGLKSLYD
                     FSEQIAAGTIAKSELSIFPIGFIAAAVSGFFCIKYLLSYLKKHSTNVFVWYRFALAAL
                     VVVVSSIRA"
     gene            11003..11479
                     /locus_tag="PLS88_05985"
     CDS             11003..11479
                     /locus_tag="PLS88_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU06924.1"
                     /translation="MILQNAFKPATIKIELESEYKDELLEELVDILAKDYPKDQLFPR
                     EEALRALRKREEKMSTGIYKGVAVPHATFEGIDKLRGVIGISKRGIDFDSLDGNPVYL
                     VFMLVSPPSQAEAHLEALRQIAILIQDPLLLENLMKATTPEKVYALIRQFEESLSP"
     gene            11489..11797
                     /locus_tag="PLS88_05990"
     CDS             11489..11797
                     /locus_tag="PLS88_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06925.1"
                     /translation="MDSADSIDRLLKIEQQASQIIQEAEEKASRLLIEAKAQSQIVQN
                     QKISQMRKELEADFAAYVDSLTQQSHKEIEEYRTSLNSIPLNQKALLETLEALLQTEN
                     "
     gene            11799..12800
                     /locus_tag="PLS88_05995"
     CDS             11799..12800
                     /locus_tag="PLS88_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOU06926.1"
                     /translation="MPETLDIAYLYARVCGAFSNMRLGEKAGELMQNSGGILALWKLL
                     FDEEPPNIPEPRLVFEAERRIIKRAIDDFVRLTEPFSDSNEFIRALISKYEISTIKSM
                     LFRMRSGEPKPEETTYFSKVIEQALARWPRVADMFSDTPYSWLDSHWLDNIAMAENEL
                     DKQYYLELWKAALGLPRRKVGAILDLIRSEVIYQNIVWTIRVRRYYRMSKEDIADTLV
                     DLPQEDTTSLALETFSYDLERVESFDSWRLKKLLSNQTGPGLDVPALEISALEDLFFM
                     VRRSLHLYPFSYTPIYCYFKLLDYETSLLLAVLESTKLGATSDEKMKYVWIPGGETA"
     gene            12797..14959
                     /locus_tag="PLS88_06000"
     CDS             12797..14959
                     /locus_tag="PLS88_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOU06927.1"
                     /translation="MSSVSMRKVELLLLKSDIDAVLKYLATKRCFQVIYPDELERLAR
                     EKRASFRALDKAESLVSNNAIREASPGEITIGKPAIGEVQFNEAHLENEQIDETQLDG
                     ERLDEAQRKLDFIGQFLGIEGPKDIIEDSRLPDEDMLSKLEVLFERCSQLKVDIDEEQ
                     AKVDGLAESIHEAKAFSSLSRPFEEFEKFSYVSIQIGKVAKERIEELSQMLGDRAVII
                     PLDEEGTILAASSRKGRFAFETALSKVGFEKKKPPEGLKGIPSETMSALEGAYQAEKL
                     RLEKLLTEKKELKDKYGDLWRTMVSSIKLKKAIALVESKLEKTRWAYRLSGWVPENMV
                     DKLVKDLLAMLGNRISIRIYDPPEQGVSGSEEKQEPVPVLLKHNPFVSAFQGIVLSYG
                     TPLYGDIDPTPFVAFFFTLLFAIMFGDVGQGMVIAGLGFAMLKAKKGLLASYKKYAHA
                     FIAAGVGSMIMGLLVGSFFTSDTALIPIERALTGLILGKPADRFLQIMPRDNVGAMFY
                     FFGFTLAVGVIINSTGLVINIINLIRRKEYGEAFFSKTGLAGALLFWWAIGIVVRIIL
                     GSKLGAIDIVGIGLPLLAIIFAEPLKAVIEKGQGKPHAEGASSWLIGGIIELLDIVSY
                     FASNTFSFLRVGAFALAHAVLSFVIFTMGSLVRGKSASGLAFEIIVFLFGNAVIIVLE
                     GLIVTIQVIRLQYYEFFSKFFTRTGEPFKPVSFAGKVE"
     gene            14999..15307
                     /locus_tag="PLS88_06005"
     CDS             14999..15307
                     /locus_tag="PLS88_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOU06928.1"
                     /translation="MKKKLLIGVMAFVVSVAAFAQTQAEPTATVSSSVQMVKYIAAAL
                     AVGIAAMGGAMAVAKIGSAAMGAMAEKPDLSGKALPYVGLAEGICLWGFLVAVLILFL
                     "
     gene            15379..15726
                     /locus_tag="PLS88_06010"
     CDS             15379..15726
                     /locus_tag="PLS88_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOU06929.1"
                     /translation="MAEIFVLAEEELLIAFGMIGIKGAAVSNSEDAAATFRSIVWDKR
                     YSREGQTFDLSDCKMLILSEDVSDMIGEELFEWQLSGAFPLIVEIPPLAGSSAQHTRL
                     VDAVRKAIGIKIQ"
     gene            15749..>16193
                     /locus_tag="PLS88_06015"
     CDS             15749..>16193
                     /locus_tag="PLS88_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06930.1"
                     /translation="MEEIRGTEALEREILEDARKRADRIIRKAEENARLLGAQTEHRI
                     KEAKAALLSEYQIKKRTAELEMLSRLPLEKARLDILYRDEILRKALKEALASMNPRLF
                     GLWCVKRLVCQVELVRKSPAKVLVHGLDSETMRDIGALFGQGSDIS"
BASE COUNT         4229 a         3664 c         4008 g         4292 t
ORIGIN      
        1 gctgcgttca aggcaagcat acctgtgctc ttgggttaca ttactttggg catagctttt
       61 gggcttacgc ttgttgcagc ggggttgcca tggtggcttg ctccatttat ggcgctcgtg
      121 atctatgcgg gcgcggctca gttcatggcc gtaggtctca tccaaagtgg ggcagggctt
      181 cttgaaattg cgcttctcac tctcattatg aatgcgcggc atatggttta tggcctatcg
      241 ctccttgagc cattcagcaa agccggcaga tggaagcctt acctgatatt tggcctcaca
      301 gacgagacct atggcctatt gaccacgatc gagcctcccc ctgatctcga taagacgaaa
      361 ttctatgctg cgatcactgt gctcaaccag gtctattggt ttgtgggctg tatggtaggg
      421 agtcttgtgg gctcggcgtt tctttttaat acgacaggcc tagattttgc tcttacagcg
      481 ctcttcattg tgctgcttgt cgatcaagta agggcggtga aaaaacccga gccctattta
      541 gcagcattgc tcggctgtgt gctggctctt ttcataagtc cgaagaattt tcttctcgtt
      601 gcgctggtga tttcgacagg cgcgctagca cttttacgag aaagaataga aggaaggcct
      661 cgcggcacag ccagccaagg cgtttcagaa aaaagcaaac aagactcaga ggagaaagga
      721 gatagaccat gacctacttc gtgctggtat gccttgtcat ggggcttgtt acctatttta
      781 ctcgtgcgct gccttttcta tttttttctg tgcgaaagcc gccggagctc ctggattttc
      841 ttcagaaata cctaccggct gttgtgatgg ctattctcgt cttttctatg tataaagata
      901 tcgattttcg cgccgcgcct tatggaattc ctgcgctcgt cgcaggggca atcactgcgt
      961 tgctacatct atggaagagg aatgtgcttt tatcgattgg agccggaact gcggcataca
     1021 taatttttat tcgaataata tgaggtagct attcggcgct catcgcaatt ttctcacgat
     1081 tcgtctaaag ccgaccttct tcgcaatctg ctcatgattt ttcaggcaac tatttgcagc
     1141 tatttatcga tattcttccc agggctttat ttctatacca tgatttttcg aatatgcgag
     1201 cgactcgata tactgacgtg cagtcttgat attcgtaaag agtggaatat tgaggtctat
     1261 ggccattctg cggatgaggt agtcgttttg aagttcgtgt tttctgttgt tttttgggat
     1321 attgatcacg aggtcgaaga catggctgcg cattagagta agtatgtttg gctctgcgtt
     1381 ctcgagtggc cagtggagtg tctgcacttc tatgccgtat ttcttgagaa actgagccgt
     1441 gccgtgtgat gcgtagaggg tgcagtccat ttcgactagt ttctgtgcgc tctcgaggaa
     1501 atcgagtttg tcttcgagcg ggcctgtcga caaaagcagc ttcttgactc tgggtgaata
     1561 gccaaccgag accatcgcct tcataaaagc atcatctagt tcgctaccta tgcagcccac
     1621 ttctccggtc gaagtcatct ccacgccgag cacggggtcc gcaccatgga ggcgagcaaa
     1681 actgaattgt gctgccttga ctccgaccca aggctggtcg tatagcgcgt tcggtggcgc
     1741 ctgtactgct tgacctaaca tagaccgcgt cgccaggtct atcatgttta tacgtgagat
     1801 cttagaacag aaggggaagg atctgctcgc tcttagatta cattctatga ccattacgcg
     1861 gttctgcttt gcgacgaatt ggatgttgaa aggaccggtg atgttgaggg cgatcgcgat
     1921 tttttcggtt attcggataa tttttcgcac cgtttcgaca tagagtcgct gtgctgggaa
     1981 tacaacggtg gcatcgcccg aatgtacgcc ggcattttcg agatgttcgg ttatggcatg
     2041 gtataatagc tttccgtgct tcgcaaccgc atcgatttcg atttctttag cgtgctctat
     2101 gaatttagag atgacgaccg gatgctcctg ggagatgtct acagcgagtc ctaggaaggt
     2161 cgcaaggctc ttgtcgtccc aagctacatt catggcagcg ccggagagca catagctcgg
     2221 cctcactaag acggggtagc caaccgatcg tgcaaaggca attgcctcct cgtcggtggt
     2281 tagctcgctc caagccggct gttctatgcc aagctcgtct aggagcgaag agaacttgtg
     2341 tctatcttct gctttgtcga tgttctgtgg cgatgtgccg agaattagcg caccagcatc
     2401 gaatagaggc agggccagat tattggcgat ctgcccaccc atggagagta gtattccaga
     2461 tggctcttcg aattcgtaga tatccatgat gcgctcgaag ttcagctcct cgaaatatag
     2521 cctatcgcag atatcgtagt cggtgctcac ggtctcgggg ttacagttga ccataatcgt
     2581 agactggcca agagtacggc atgtctcgac ggcgctgacc gcacaccaat cgaattcgac
     2641 gctggagcct atgtggtagg gaccgcttcc tatgacaagg atgcttccat gtgatggttt
     2701 tacgtcatcg gatggagcga taaccgaatt gcgcgataag gattttgatg caaatgaatg
     2761 agcgtaggta acatagagat aattcgtttg ggcagggtat tctgcggcca gggtatcaat
     2821 ttgacgcacc gaaggccgca gtccaagaat ctgacgccaa tgccgcactt cgagttcgtg
     2881 aatgccaaag atgtgagcaa ttcctgcatc cgaaaaacca aggcgctttg cgcgcacaag
     2941 tattgtggcg atcttgtcat cgagttttat cgcggacgat gcaacccgag cttgtctggc
     3001 tgtttgtgac accttgcgca tttcgaattc gcattgtctt atagaatcga ttttaccaat
     3061 gaaccaggga tcgataccgg tgagcctatt gattttttgc acgctccagc cttcctcaag
     3121 agcttgatac actgcaaaaa gccttttgtc tgtaggcttg gagagcgaag cacgaagcct
     3181 gtttgatgct atagctgttt ggtttccatc gctctgtaga ccgaactggc ttgcgcttgt
     3241 catgcgaata gccttctgga gcgcctcctc gaagctacgg ccgatggcca tcacttcccc
     3301 tacggacttc atctcgctgc cgatgtgttt gtccacttcc ttgaattttg cgagatccca
     3361 gcgcggcatt tttatgacac agtaatcgag ggcgggctcg aagcaagccg ttgtgacccc
     3421 cgttatctta ttgcgcagat cggttaggca aaagccgagc gcgagttttg cggctacata
     3481 ggccaagggg taacctgtcg ctttggaggc gagagctgag ctgcgcgaaa gtcgagcgtt
     3541 gacctcgatt atgcgatagt cgccagaatg cggatccaag gcgtattgga tattgcattc
     3601 acctataatc cccaagctcc tcacaagctt gagggctatg cttctgaggt cgtggtattc
     3661 cctgtcggtc aaagtctgcg agggagccac gacgatgctt tcgccggtgt gaatgcccag
     3721 tggatcgatg ttctccatgt tacagatcgc aatacagttg tccgccttgt ctcgcacgat
     3781 ctcgtattcg atttccttcc aaccaccgag ccattcttcg ataagcacct gcggagaaag
     3841 ggcaagggct tcttctgctc gtgctcgaag ctctttttcg ttcttgcata gtccggagcc
     3901 agcgccgcca agtgcaaagc ctgccctgag cattacaggg tatcctatgg tgccgccaca
     3961 tacgagcgct tcttcgatat tcgtagctgc cttgctctcc ggcgtcttga gtcctagttg
     4021 tcgcagatga ttagcgaaga gctcgcgatc ttcggttagc tcgattgctt taatcggtgt
     4081 accaagtacg tctacagcgt acttatagag aatgccggct ttgtgcagcg ctagcccgca
     4141 gttgagggcc gtctgaccac caaaagatag tagtatccca tcaggcctct ctttttcgat
     4201 gacttgtgcg acaaattcgg gagtgagggg taagaaatag gtggcatctg cgacgccttc
     4261 gctggtttgg ttggtggcga tgttgggatt gataaggatt gtaaagatgt tttcttctcg
     4321 gagagccttg agcgcctgcg atcccgaata gtcgaactcg cccgcttggc caatcttgag
     4381 gccgcctgag ccaagcacca ggactttttt catcatgctt gcctcttttg cctttttgcc
     4441 tcagctactt catggaggaa ttcttcaata aggaattgag tatctcgcgg acctgggcag
     4501 ccttcaggat ggaattgcac ggcccgaaaa ggtgcatttg aagctcggat gccctcgatt
     4561 gtgccatcat ttccattcaa gaaccagggt tcccagcccg gtggcaggga atcttcaagc
     4621 actgcataac catgattctg gcttgttata tagcagcgcc cgcttctcgt atcgatggca
     4681 ggctgattct ggccgcggtg gccatatttt agcttatagg tatcggcccc tgcagcaagg
     4741 gcaaggagct gtgttcctag gcagatgcca aaaatggggc gtgtttctcc taacgcatgc
     4801 cgcagatgcg caatagtctt tgtgcaggcc ttagggtctc ctggcccatt cgatagaaaa
     4861 aggccatcat actcgatgtt gttcagagga taatcccagg gcatactgat cacttcggct
     4921 ccggcatcga gaagaatacg cataatactt gctttggcgc cgcaatctat aagcgcaatg
     4981 gttggtcttt cgcttatctc taggctattt actatagagt cttgtttttt cagctcattt
     5041 ttcggtgagc tatccttgga ctgagcatct tcttgtctat atctctttag actcgatgga
     5101 gagacgagtt tgactgggtt gttgatcttg atttcgtctc taataggctg cggagttcct
     5161 tcgacgagca ccttgccgta catagttcct tcttcgcgca aaacgcgaat cagccttctt
     5221 gtgtcgattc ctgcaattcc aggaatattc tcctctttga gccatctaga gaaagaccgt
     5281 tctgcgctgt aatggcttgg ttcggaagaa gcctctgcga ccacaacggc accgacttga
     5341 attttatcgg actcgaaatc gataggaatg ccaagggcat tggtgcgcag gcgaggcacc
     5401 ccatggtttc ctataagcgg ataagcgaac acaagaatct gtccacaata cgaagggtct
     5461 gtcaaagact gattgtaacc gaccattcct gtcgtaaata caacctcgcc ttcttcggag
     5521 gtatcagcac cgatcgaata tccttcgaat attgaaccat cttcgagcac aaggcatgct
     5581 gatttgaaat gtacgggcat agagcttcct tgcttccttg ttagcttttt acaagctttt
     5641 tgcgacttta tccaaaaatt atgaatattt caaggactat atgcataaaa ataggataaa
     5701 tatacaaaaa taatgtagaa taatatatcc atcgcaatag tcaatcgaag atgaccgcca
     5761 tgaggctcat atagagagga atcgtgatca tcgatgcgac agtagtcagc gatgttccga
     5821 tggctgcata ctcggaatcg gatttataag tttctgcaag gataggtgtc tgggtcatgg
     5881 ctggcatcga ggcctgcatc acaaatactt ccttcatgag aagcggcata tcgaggctcc
     5941 tgacaaccat aaacattaat gtgggcgtta tcaaaaatcg cccagcgagc accgctataa
     6001 aatctttttc gaacttcaac tttttccagt ctactcgcgc aataacaata ccaatgaaca
     6061 gcattgaaag aggcgtagtc atattgccca ggtatttgca ggtatccata atggacttcg
     6121 gaagtgttat tcccaccaag ataaagaata tcgcaatcaa gaagcctatg agaggaggtg
     6181 aaagtatgcg ttttagcccc gatagcgaga caagcgaggg cattgggctc ctattgcgca
     6241 ccgcaccatc cattgaaatg ccatatactc ctatggtcca aaagagtgtt gtgttagcta
     6301 tatagtagag gagtgcatag ggtaggcttt tatccccaaa gagcaaaaga ttgaccggca
     6361 gccctatgaa tatagaattc gataacgcaa acatcgagcg aaatgtgcct tttctgtcct
     6421 cttttacctt gaggattttg cctaccatta tggcgatgag gtagcttgcg atcataacgc
     6481 caaaaggtat aactaggcca ggcagcatcg agagtagtgt tggtttgtca tatcctcccg
     6541 tgagattcga tatcatataa gccggtagcg cgacattgac cacaaggcgg gaaatgagag
     6601 agtttccacc accttcgaac catcgtgcct ttgaaagtcc aaatccaatc gcaatcatta
     6661 tgattatgga gaatacgctt tggagagcat ttaacatggt tgttgtccgt ctctttacgc
     6721 tatctgcttt ctatttttaa gacaatcgta tcataatcca tactatttct cttgatcggt
     6781 ctgggcatcc agcttttttg aaaaatcccg ataaagcgca aaggcaaggc caaccgacag
     6841 tgtgtcgaca atcggttgta ctgtcataag gccatataga gggatgagcc gatccataag
     6901 aaacatgagc ggcacatcga tagtcgtttt gcggaatacc gatattgtga gagctcgttt
     6961 gccttgacct gtcgcctgga acatagtcgt aattaggtag cttatcgcca tcatgggcgt
     7021 cataaggcag agaattcgta aaaaacttgc gcccagcctt acggtctccg gatcattaat
     7081 gaagagcctt acaataggtt ctgcaaaaag ttcaaatata agtatacaga atagagaaaa
     7141 gcaaacggcc acgattcgtg taaatttatt aacctcgtgc actctatcgt atctctttgc
     7201 gccataatta tatcctataa aaggaagtac accttgagag attcccatcg tcacattcat
     7261 aggcaacata tcgatctttt ttgcaattcc gattgcggca agggcggcgg aaccatactt
     7321 tgcagtaagg ttgttgagaa ccgtgttgga agtaagggat agaatattct gtagagatgc
     7381 cggcaatccg acagatacca cttttgctgc aatactgaat tcttggtcta gcttacgtgg
     7441 aacaaaggag atgatggttt tgcctttctt gtgggcaaga tacacgagga aatagcatac
     7501 agccgcgcaa ttcgatatca tagtggcgat agccgcgcct ttcacctcaa ggccaagcgt
     7561 aaagatgaat attggatcca aaattatatt gaggatgcct ccaaaggaca ttccaatgct
     7621 cgcttgtctt gcgcctcctt cggagcgcac caggtgtcca aaaagcatgc ccattacagt
     7681 cggtatgcca ccgattacca aaacccatat tatataattg ctcgcaaaat cgtaggtctc
     7741 gtggttagct cccaggagca ggagcaatgg cctctgaaag ataaaggcta gtatcgaaaa
     7801 gatagcggcc acgaaaatag cagaaatcaa gcaaaagcta gaggtctgtt tggctttggc
     7861 gggttggccc gaacctagaa atctagaaat gacgctactg ccaccgactc cgaagagatt
     7921 tgcaatagcc gtcaacattg tataggcagg catgacgagc gagaccgcag ccacctttct
     7981 cgggtcgccc agttggccga cgaagaaagt atcggtgaca ttgtagatca ttgtcacgag
     8041 ctgacttatt acggttggta tggcaagatt ggccaccgcc gatggaatcg aagcactacg
     8101 gaatacttct attctcgctg catcgtgggc tggagctaca tatacgcttt cgtgttgaac
     8161 atcttccaaa tcgcacagac cacctttctt gttatttctc tatttcgagg aagaacatag
     8221 ccccatagat tatccgcata ctcaaataac ttaaaggggt atggcattcc ataaaaagtt
     8281 tcttatcgca gatatccaag ccattgtgcc tgtcgcgcaa tcatcacatc cactaactct
     8341 ggcactcgtt cgcagatcgg tacaactgga ttgaccagaa gggcttgaat tacgaggtct
     8401 ttgcttttat ggagaatcgc ttctgcggtg agatcgtaga cgccagtgta attgcgcagg
     8461 agagctgcaa aagctctcgg ataattggga aatgctacac cttctaagcc ctttctatgc
     8521 acaagcgcag gtacttccac agttacattt tggggcaagc ttggaatgag cccattgttc
     8581 ataatattga cagctgcttc ttcgtagccc gagtcttgga gtataccttc tataataggt
     8641 accaagcgct ctttaacctt gagctcgatc tttggctcga gcatactcaa ggagctgcga
     8701 tacagcaaat agaaatcgag tattccctta ttgtccgcga catcgtgagc ccaggggata
     8761 tattctccaa tatggctgtc ggaggtgata ggcagaagat ggaaacgctc caagatttgt
     8821 cggaagagcc ttctgtcggc ccaatgcctc gacgaaacag tacgaccaat attgggcaaa
     8881 tccgtttcgc cttcggtttt aggtatattt ccagtctcct gtatgtattc cagtatatct
     8941 gaataaccaa tcattctttc gaaaaagtct ggcgctttgg caagtatttc gggataggca
     9001 tcatctcctg tgtcgcgata gcgagcagat actaggacgc tgaaatggtt gagtccagca
     9061 gctctgagtt ctagcttatc aagagggaca ccgagcattg gcgggatata gcgcgcgagc
     9121 gaggctattt catggcatag tccgatgaac ttaagctgtg ggaattttcg caggactgtt
     9181 gttgttatcg aagtcatcgg attggagaaa ttgaagatgg tagcattggg acagagctct
     9241 gtacaatcct gacaaatatc gagaataggt ggcacgattc ggagtgcatg aaaaagcccg
     9301 cctgcgccgc cattctcgcc atagacctga ggtatgccat actgcaaggg gatcatccaa
     9361 tcttgatccc agagctcgaa gcggttgcca acttcgatgg agataacgac aaaatccgcg
     9421 cccttaagag cctctcttcg aactgtgtgc gccgacagtg taaaatccag gcaatgagcc
     9481 tcgacaaagg tctgtgcggc tttataaacg cggctaagag tctctccatc gatatctacg
     9541 agagcaatat gagaaccttt gagtaagctg ctcgaaaaaa tatcgcctag agtaccgaat
     9601 ccgaactgtg cgctgccagc gccaataagt acgattttca ttgctattcc tccaataatc
     9661 tcgatccaaa atattttcaa aataggaaga ggaggcgtca aacgctttat ttctcaatga
     9721 attataagcg attcaagacc gtctccaatc caaacattca aaaagaaaat ttcaatccat
     9781 caatcaatta tctgcatcaa tcaattatcc gaaaatagcc tatgcccgaa tcgatgagac
     9841 taccaccacc aaagccgcaa gtgcaaatcg ataccatacg aatacattag tcgagtgctt
     9901 cttgaggtaa gacaaaagat atttgatgca aaaaaagcca ctcacagccg cagcaatgaa
     9961 tccaataggg aaaatagata attcgctttt agcaatggtt cccgcagcta tctgctctga
    10021 aaagtcatat aggcttttta gtccagctcc agcaataatg ggcgccgaca agagaaaaga
    10081 aaaacgggct gctgcttccc tttcgagtcc aagtgcaagt cctgccgtta tggtcgaacc
    10141 agagcgtgac acacccggaa tgactgcaaa agcctgcgca aggccaatgc agagagcgtc
    10201 gcgagctttt atctggctaa aattcctctg atggcgtgca tatttgtctg cagtccagag
    10261 aagaagcgca agtagagcga tagatccggc cataaatagc atagaactcc gcggtatggg
    10321 gtcggaatga aaagcttcgt ttatcttaga ttcaagcaag aggcccgaaa ttcccccggg
    10381 aatacatgcc agcaagatgt accaggccat acgccgatca ggatcgtcgc ctattttgaa
    10441 ttgaattacg ctcttgaacc aagccgagat aagccgtgcc caatctggag cgaaaaacac
    10501 aattactgcc aggagcgttc cgagatggag cgctacatcg aaggtgaggc ttgtaagtgc
    10561 agggttgtcc catccaaaga gccatggaac gatgataagg tgtgctgagc tcgaaatagg
    10621 aatgaattca gcgaggccct ggatgattcc aaggactatt gattcaataa tcagcatgta
    10681 tttcccttgg gtgagtgcta tagcatctta atttttactc tgtttaaggt ttatctgcaa
    10741 tatggcaatg cagggttaaa ataaaagtcc tcggttcttt tttttcctta tccatatcga
    10801 agcttctata tattcttaat attcttagat atgcgtgtta gaaacgcgtc ttagatgtgc
    10861 tacgcgccga caattcaatt tgggtacacg acaattattg gcattcaatt ccgctgcctt
    10921 gccatccagg gtatctttca ctatcattta agctaatctg cctaaaccta tcgataaggg
    10981 aaacaggaga ggggaagttg cgatgatact ccagaatgca ttcaagccag ctacaataaa
    11041 gatcgagctg gaaagcgaat acaaagacga acttctcgaa gaattggtcg atattcttgc
    11101 aaaggactat ccaaaagatc agttatttcc gagagaagag gctttgagag cgcttcgaaa
    11161 aagagaagag aagatgtcta ccggtattta caagggagtc gctgttcccc atgcaacatt
    11221 cgaaggcatc gataaacttc gcggagttat tggtatatcc aaaaggggca tagacttcga
    11281 ctctctggat gggaatccag tatatcttgt gttcatgctc gtttctcctc ccagccaagc
    11341 tgaggcacat ctcgaagcct tgcggcagat tgccattctc attcaagatc ctctcttgct
    11401 cgaaaacctc atgaaggcta cgacgcccga gaaagtatat gcccttattc gccaattcga
    11461 agaaagtctc agtccatgag gtcatgttat ggattcagca gatagcatcg atagattgct
    11521 caagatagag cagcaagcat ctcagataat tcaggaagcc gaagaaaagg cttccagatt
    11581 gctaatcgaa gcaaaagccc aatcgcaaat agtccaaaat cagaaaataa gccaaatgcg
    11641 caaggaactc gaggcagatt ttgcagccta tgtcgattct cttacccaac agagccataa
    11701 ggagatcgaa gaatatcgca caagccttaa ttccattcct ctaaaccaga aagcgctttt
    11761 ggagacattg gaggctctcc tacaaacgga aaactgatat gcccgaaacg ctcgatatcg
    11821 cctatcttta tgctcgcgtc tgcggagctt tttcgaatat gagactgggc gagaaggccg
    11881 gagagctcat gcaaaactcg ggaggtatcc tagctctttg gaaactgctt tttgatgagg
    11941 agcctccgaa cattcctgag ccaagacttg tcttcgaggc ggaacgcaga ataataaagc
    12001 gcgccatcga tgactttgtt cgcctaacag aacccttttc cgattccaac gagtttatcc
    12061 gcgcattgat atcgaagtat gagattagca ccataaaatc gatgctcttt aggatgcgtt
    12121 ctggcgaacc aaagcccgaa gagacgactt atttttccaa ggttatagag caagccctag
    12181 ctcgatggcc tcgcgtcgca gatatgttct cagatacccc ctattcatgg ctcgacagtc
    12241 attggctaga taacatagca atggcggaaa acgagctcga taaacaatac tatcttgaat
    12301 tgtggaaggc agcgctcggc ttgccaaggc ggaaagtagg agcaattcta gatttgatac
    12361 gctcggaagt catctatcaa aatatcgtgt ggacgattcg tgtacggcga tattaccgaa
    12421 tgtcgaaaga ggatatcgct gatacgctcg tcgatttgcc gcaagaggac actacttcgc
    12481 ttgcgttaga gactttttcc tatgatcttg agagagtcga gagtttcgat tcttggcgac
    12541 tcaagaaatt attgtcaaac cagactgggc caggtctcga tgttcctgct ctggagatca
    12601 gtgctctgga agacttgttt tttatggtga ggcggagtct gcatctctac ccatttagct
    12661 atacgccaat ttactgttat tttaagcttt tagattatga gacatcgctc ctcttagcgg
    12721 tacttgagag tactaagcta ggcgcgacct cggatgaaaa gatgaaatat gtgtggattc
    12781 caggaggaga gacagcatga gcagcgttag tatgcgcaaa gtcgagctat tgcttctcaa
    12841 gtccgatata gacgcggtcc taaaatacct tgcaactaag cgctgttttc aggtcatata
    12901 tcccgatgag ctcgagcggc tagcgagaga aaaaagagca agttttagag cgttggacaa
    12961 ggcagaatcg ttggtcagca acaatgctat ccgtgaagca tctccgggcg agataacgat
    13021 aggcaaacct gcaatcggcg aagttcagtt caacgaggca catctggaaa acgaacagat
    13081 cgatgagacg caactcgatg gtgaacgact cgatgaggca caaagaaaac ttgattttat
    13141 aggccagttt ctcggtatcg aaggcccaaa agatattatc gaggacagcc gattgccgga
    13201 tgaggatatg ctttcaaagc tcgaggtgct cttcgagcgc tgctctcagc taaaggtaga
    13261 tatagatgag gaacaggcga aagtagatgg gcttgcagaa tctatccatg aggcaaaggc
    13321 cttttccagt ctttcgcgtc ctttcgaaga atttgagaaa ttttcctatg tatcgattca
    13381 gataggcaaa gtggcaaaag agagaattga agaactttct cagatgctcg gagatagggc
    13441 agtcataatt cctctagacg aagaaggaac tattcttgca gcctcgagcc gcaagggtcg
    13501 ctttgctttc gagacagctc tctctaaagt aggattcgaa aaaaagaaac cgccagaagg
    13561 cttaaagggc ataccatcgg aaacaatgag cgcactcgaa ggagcctatc aagcggaaaa
    13621 gctccggttg gagaagcttt tgaccgaaaa aaaggaacta aaggataagt acggcgatct
    13681 atggcgaaca atggtgtcct cgattaagct taagaaagct attgccctgg tggagagcaa
    13741 gctcgaaaag acgcgatggg cctatcgcct ttcaggatgg gtgcctgaaa atatggtaga
    13801 taagctggta aaagatctac ttgccatgct tgggaaccgg atttccatcc gcatctacga
    13861 tccgccagaa cagggcgttt ctggttcgga ggaaaagcaa gaaccggtgc cggttctctt
    13921 aaagcacaat cctttcgtat cggcgttcca gggcattgtc ctatcctatg gtacgcccct
    13981 atatggagac atagacccga ccccctttgt tgcattcttt ttcactttgc tgttcgcaat
    14041 aatgtttggc gatgtaggcc agggcatggt gattgcaggt ctagggtttg cgatgctcaa
    14101 ggcaaaaaaa ggtcttcttg cgagctacaa aaaatatgcc catgctttta tcgcagccgg
    14161 cgttggttcg atgataatgg ggctgcttgt aggttctttc tttactagcg atacggctct
    14221 cattccgata gagagggctt tgactggttt aatcttgggc aagccagcgg atcggttttt
    14281 gcagataatg cccagagaca atgtaggcgc catgttctat ttcttcggat ttacattggc
    14341 cgtgggagta ataatcaact ccacagggct tgttataaat ataattaacc tcatacgcag
    14401 aaaagagtat ggtgaagcct tcttttcgaa gactggcctt gctggcgcgc tcttgttttg
    14461 gtgggctatc ggcattgtag ttagaataat tttgggaagc aagcttggtg caatagacat
    14521 tgtaggtatt ggactgccgc ttctggccat tatattcgcc gagccattaa aagccgttat
    14581 cgagaaggga caaggaaagc cacatgctga aggggcttcg agctggctaa taggtggaat
    14641 tatcgaacta ttggatatag tcagctattt tgcctcgaat acatttagct ttttgagagt
    14701 aggagccttt gccctagccc acgccgtgct gtcttttgta atatttacga tgggcagtct
    14761 tgtgcggggt aaatcagctt ccggactggc attcgaaatc atagtctttt tgtttggcaa
    14821 tgcggttata atcgtgctag aaggtctaat tgtgactatt caagttatca ggcttcaata
    14881 ttatgagttc ttttcgaagt tctttacccg aacgggtgag cccttcaaac cagtaagttt
    14941 tgcaggtaaa gtggaatgaa gccatgatct ataacattat gttagaagga gtttttgtat
    15001 gaagaagaaa ttgctaatcg gcgtcatggc atttgtggtt tcagttgctg cgtttgcgca
    15061 gacgcaagct gaacctacgg ccacagtatc gtcctcggta cagatggtaa agtatattgc
    15121 cgctgctctc gcggtcggta tagcggctat gggtggagct atggccgttg caaagatagg
    15181 ttcggcagca atgggagcca tggcagagaa gccggatctt tcaggaaagg ccttgcctta
    15241 tgtcggcctt gccgaaggaa tttgtctttg gggatttttg gtcgctgttc ttattctatt
    15301 cctatgaggg agaatggatt ggcataaact ccaaggcaag gcttctgcat acgagagggc
    15361 ataatactag agggcaatat ggcagagatt ttcgtgctgg ctgaagaaga gcttctcata
    15421 gcttttggca tgattggcat aaaaggcgca gccgtatcca acagcgaaga tgctgcagct
    15481 acatttcgaa gtattgtgtg ggataaacgc tattcgcgcg aaggccagac ttttgacctc
    15541 tcggactgca agatgctcat actaagcgaa gatgtatcgg atatgatagg cgaggagctc
    15601 tttgaatggc aattgagcgg agcctttcct ctaattgtcg aaattcctcc tcttgcaggt
    15661 tcttcagctc agcatacgcg ccttgtagat gcagtgcgga aagcgatcgg aatcaaaatc
    15721 caatagcaaa gcagaattag gaaaaagcat ggaagaaatc agaggcacag aagccctaga
    15781 acgggaaatc ctcgaagatg cgcgcaagcg tgccgatcgg ataatcagga aggccgagga
    15841 aaacgctagg cttcttggcg cccagaccga gcatagaata aaagaggcta aagccgctct
    15901 tttgagcgaa tatcaaatca aaaagaggac tgccgaactc gaaatgcttt cgcgtcttcc
    15961 tctcgaaaag gcaaggctcg atatcttgta tcgcgacgag atacttcgca aggcgctaaa
    16021 ggaagccctt gcaagcatga atcctcgtct ttttggcctg tggtgtgtga aaaggcttgt
    16081 ctgccaggtt gaactcgttc gtaagtcgcc cgcaaaagtg cttgtacatg gcctcgattc
    16141 agagacaatg agggatatcg gagcattgtt tgggcaaggt tcggacatct cga
//
LOCUS       DAOSOB010000046        16173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3628_length_16173_cov_86.046656, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000046 DAOSOB010000000
VERSION     DAOSOB010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 16173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16173
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3628_length_16173_cov_86.046656"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..837)
                     /gene="pgsA"
                     /locus_tag="PLS88_06020"
     CDS             complement(223..837)
                     /gene="pgsA"
                     /locus_tag="PLS88_06020"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOU06931.1"
                     /translation="MSLADKITLSRIILAPFFFFFIKAEFIPREPAIIVLWILFAWME
                     ISDLIDGKIARSSNHVDSFGKLFDPFADVISRVTYFLAFASIGIMPLWVLLIVMYREF
                     GILFLRALLALKGTAMGARPGGKLKAGSYMVAGLLSLGLYTVQLYASNMNGVAFGSFS
                     NHSSEMAGLASVLQTLTYISYVVVAIATVLSFVDYLVQFKKLYK"
     gene            967..1761
                     /locus_tag="PLS88_06025"
     CDS             967..1761
                     /locus_tag="PLS88_06025"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOU06932.1"
                     /translation="MEERDRISGLRRNILLTMSIFFVVQCAIFLTFALVVGFESQYWT
                     IFLPISTGFHILILILLLLFQNDFYIESTGQKLDRINLANIITLSRVSSMPTLLVLVI
                     AAKEYSIRLPVLILIALIFLTDFFDGLISRKTNQVTKIGRIMDSVSDYALLIVLSIIF
                     QYYLLIPGWLFWLIIARFGIQGLLMAVLMIIEKRIEPKSTFMGKVTIASIMVLYTLEI
                     LRVILQAPSHLAFRIAEWIVAIIIVIGIFDKIASFFDTLKSKPSAP"
     gene            1797..2438
                     /locus_tag="PLS88_06030"
     CDS             1797..2438
                     /locus_tag="PLS88_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06933.1"
                     /translation="MAVIKSALELALERTKDLQLDENAQKIADAKIEGRKAASRYLED
                     PASVDFKAILSTLDPVQRQAFLSSAFEVLSNFIQLPTNSVVDTEKMEAIGKAIVLLCG
                     LSARFPSEKEVKLAQQQARSLFQQILRFLSQYQEEMKRVEQAIRNQWAPKLKEREKQL
                     AAALGQNVRMDPMSDPEFAEFYRKNIESMRNNYGKALEDAKSQLADICGFEAQ"
     gene            complement(2464..4344)
                     /gene="mnmG"
                     /locus_tag="PLS88_06035"
     CDS             complement(2464..4344)
                     /gene="mnmG"
                     /locus_tag="PLS88_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOU06934.1"
                     /translation="MGDFEAIVIGGGHAGVEAALALARLGTNTVLITQNPDTIARMSC
                     NPAIGGLSKGNLVREIDALGGQMGILADAAAIQMRMLNRSRGAAVQAPRAQADKALYA
                     KLARQALENQPNLTIFMDTVVDFILSKGTHPRIEGVVTERGNTIGARVVVLTTGTFME
                     AKLFIGAWSGPGGRLGEPAAIGLGTALRAKGFPLGRMKTGTPARIKRGSIDFSKLEVQ
                     RGDKAKIFFSFLEHDYERPDVPCYVVYTNAATHEAIRSGLDRSPLFSGRIVGKGPRYC
                     PSIEDKVMRFPDRDRHQAFIEPEGLSTDEMYLNGLSSSLPEDVQERFYHSIVGLENAI
                     IVRPAYAVEYDYIDPAALYASLESKLVEGLFIAGQTNGTSGYEEAAAQGLMAGINARR
                     KLDRTSPLILGRNEAYIGVLIDDLVTLSPKEPYRMFTSRAEYRLSLRHDTADLRLTPY
                     AIETNLVDLQRRESFERRLRTIEEAETLLQSHKISHEDIAQMPELEGHLGQALSDALR
                     DPKVGCLYDDETEALARLAKILPESMGMQASGVITALLNERYKGYQEKENRLAHRLAR
                     AEKLFIPQGFDYKAVKGLSQEALEKLSSSQPLTLGQASRIPGVRKSDIALLYIALNKP
                     QR"
     gene            complement(4485..5909)
                     /gene="mnmE"
                     /locus_tag="PLS88_06040"
     CDS             complement(4485..5909)
                     /gene="mnmE"
                     /locus_tag="PLS88_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOU06935.1"
                     /translation="MSRNYFDRDTPIVALATPEGRSALAIIRTSGEAAIDIVARCFSR
                     PEALLGAAGYQAVHGWIIDPLSNERIDEVMALVYKAPHSFTGENAVEILCHGSAAVVR
                     RILDLLYMQGFSPALQGEFSFRAFIEGKTDLVRAEAINELSLAFCEAARHDALSRLSG
                     VLSKRLKELRSMMLDVLADIDARLDYSEDEGPEEIERWPMIISQIQGALAELLKSYPG
                     GKLRQEGLLVVIAGRPNAGKSSLFNLLVREERAIVSEEPGTTRDWLESWITIGGLAVR
                     LVDTAGLRDSKNEIEIEGVRRAKAMLDRADVIFYLVDGTVGLYEEDKNFMAAYPGALF
                     LWNKVDRMDCLSAPAGWKAISAKDVASFGFLESVLVDELRAKIDKGYIPQSSEPQYQK
                     SESGEPQNQELQVRIASERQKQLLSEAQEALNRAMEAYEAGVALDLVSLHIKEAAESI
                     GEITGDIVSDEVLERIFSCFCLGK"
     gene            6035..6688
                     /locus_tag="PLS88_06045"
     CDS             6035..6688
                     /locus_tag="PLS88_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06936.1"
                     /translation="MTPQELSRLDYEGASQTILAYTIDAKKYEKEIAALKSQAQEWRV
                     KAAEAQTKAAPELSEAASKRASELESKAQQLSVELVAIRRDIEQLREALPIIKAKQRR
                     VDPDQLLAELSMIVGLDALGDNKESGPKSDSTTSNVDKDSGAVKDQDSSDNKIPASPM
                     NPVSSVKPASPVTPTSPANSESFANPESTQNPSKAASLADQALDDALAELKKKMGLL"
     gene            6748..7596
                     /locus_tag="PLS88_06050"
     CDS             6748..7596
                     /locus_tag="PLS88_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOU06937.1"
                     /translation="MKMRHLPVLPEFPRPLLFAHRGLNRKYLENSMESFEAAFNAGVP
                     GIELDVHLTADDKLVIFHDDTTSRIEQKIQPEAPARNLELEASTFVELRSLFIGAHIS
                     LLDELFDCFGEALYYDIELKSRSTADTGLASKVASAIRSHNLESNCIISSFNPFALRY
                     FRQAESAVPVGIIWSNSDELYWFLRHGEGALISKSDFLKPESSLFANMAGRPPLHLLP
                     WFRGSKEKPIIPWTVNDPEKACYFIDQGSTGVISDIADEILIRFRAEHEQAAAADPSQ
                     KLSLKA"
     gene            complement(7523..9244)
                     /locus_tag="PLS88_06055"
     CDS             complement(7523..9244)
                     /locus_tag="PLS88_06055"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOU06938.1"
                     /translation="MKISLIQLNSKIGDFKGNADRVIAMVNRMLSMFPALERVDLVVF
                     PELCLCGYPPMDLLDQESFLLGSIAAIRMLQKMLPSDVAVGVGYVDRNRNGTGKSLVN
                     AYSIIQDGKVVFTQAKTLLPTYDVFDEARYFEPAKERNLFVQPWGKVGFAICEDFWWE
                     EPNASQEYAIDPVKELMDEGADIIVVTSASPFIAGKLETRIRLAEKAVRAGYIPVLYC
                     NAVGANDSLVFDGRSFAMGEDASICGICGWAEEALIYDSVSGKANRMLFDPLLQKAEF
                     VEENQLPCPDRYEEIQKAIVVGIKDYLRKSGFSKVVLGLSGGIDSALVAVLAAQAIGR
                     QNVTCIAMPSRFSSEASLDDAVELCRRNKLRLERIPIEVPFTAYLEALSVPFAGKPYD
                     TTEENLQARIRGTLLMAWSNKFNALLLTAGNKSELATGYCTLYGDMNGSLAPIADLYK
                     TEVYGLALYINRVAAENGDIEPIPEPIIAKAPSAELRYNQKDQDTLPEYEVLDKILQL
                     YIEENLSMDQIVNLGFDRGTVRKTLEMTGKAEYKRRQAAPAIKLSKRAFGLGRRLPLA
                     RALHEIE"
     gene            complement(9241..10365)
                     /gene="lgt"
                     /locus_tag="PLS88_06060"
     CDS             complement(9241..10365)
                     /gene="lgt"
                     /locus_tag="PLS88_06060"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOU06939.1"
                     /translation="MVAMLEFPKWLSPEIIPGLPFRWYGLMYLVAFGVTWLLFKRESK
                     RLGAPWTEDEAANFFIWGIVGILLGGRLAGTLIYEPTNYYWIKPWYIFWPFDESGTFV
                     GFQGMSYHGGFVGLIVATLIWCKVHKWNWFDWADLIAVSAPLGYTFGRLGNFINGELW
                     GKVSTAPWAMVFPNAERFSAKEQWVQEIARKVGIRIVSMNDMVNLPRHPSQLYEALLE
                     GIVLWLILWFFVRPRRSFRGEGVAAYSLGYGIARFFVEYFREPDSGLGYILALGDPDA
                     PMALFVSPFNFSMGQILSFIMIIGALIFLFYAKKHFKIEPGEPIFIDASRAGGEVNAA
                     QARKGGGASRASGSGNARSTKYDASSARKLRKKIKSGGER"
     gene            complement(10365..10850)
                     /locus_tag="PLS88_06065"
     CDS             complement(10365..10850)
                     /locus_tag="PLS88_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06940.1"
                     /translation="MQRSLFLLIAAIVAFLRIFIIGIVAILFGMSDYGGVTSALLRIF
                     QLYNLLFVYIVLIQYIKAGAREYLRKPSLIVACVSPILSVLALLTYIRNIGGSFPVDM
                     AKAATSMITLILLDIVVLGLLVIDALKFKLPEAKKAENPENMEKTEIMDKTHKDQHEV
                     S"
     gene            10973..12442
                     /locus_tag="PLS88_06070"
     CDS             10973..12442
                     /locus_tag="PLS88_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU06941.1"
                     /translation="MKHIDVPAIRKQIISFVSLAVVLALSFVTVSVPELYAQQLKTPD
                     SKQYSLLLQNIYHFILQNYVEEPDPTKLYEGAVKGMLDSLNDPYSTYLDEDMMSDLMS
                     DTTGIYGGIGLYISKQATVANENAPRYIEVVSPIEDTPAWREGIRPGDLIIKIDGEDT
                     SPLSTDKASAKIRGEAGTTVTLTFRRGTYEFEITFTRTKIEIPALKSALIQTANGNVG
                     YIRIIEWNANTAPQMKTVLQDMKKQSIDKFILDVRSNPGGLLSSVVDVSDLFLSTNVI
                     VSTKGRSSSENYEYKAKPDLAIPATDRMIVLIDQGSASASEIFAGAMKDTKRALLVGE
                     KTYGKGSVQQIFPLDKGGFKLTMAHYYTPSGIDIDKVGIEPDVKVPEPTLNDKELEEL
                     QRLYDAGDIARYLESNPQPTLADRKAFAAELAQKYKIQEIFIEKIIRDEAERSMPARI
                     YDLEYDIQLQKALDFIESADFEQMLENTKTLAQVRALSN"
     gene            12458..13282
                     /locus_tag="PLS88_06075"
     CDS             12458..13282
                     /locus_tag="PLS88_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOU06942.1"
                     /translation="MRQIVLLGNQRGRETCSLDEKTSHYLINVRRMRKEDSFNAVDEA
                     GSRFRCIIISDDPRAVKLGITPISSPRSLVSSASNTAEEGQPKIGIAPTLHIALVQAL
                     PKGNKFDLIIRQAVELEVELIIPLITQHCVEQEPSERANAKLERRKRIIREAVQQSGS
                     LVYTEILSTANLETLDAVLNAKGFSKKDSVRILFHESEQEKSQSLHLLLSGDKNSVVL
                     AIGPEGGLSPNDCAAFIDMGFAVHHARGPVLRVETAAIFAISAVRAIAFERGLWKV"
     gene            13270..13977
                     /locus_tag="PLS88_06080"
     CDS             13270..13977
                     /locus_tag="PLS88_06080"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOU06943.1"
                     /translation="MESVSIACAFLDGLRLDFPSQFDLFTLPYLWDDEKNHQICFVDK
                     QSWIHAKKRDEFGSMIASADMILNRDSGAAESVLMRGEKDSRCWPVPFIHQVLLDAIK
                     ESMEEENSARLENPGEETKAYRPQKVLTLLLSSLEKRNGSVFLLGGNPITLIKAEKNI
                     RATYPGITIVGSMHGLYRPEEESAIIEAIQKGAPQLILAGSPLPDGECWIPRHMNITR
                     SGIFFYYKPIMKWISGR"
     gene            14114..15256
                     /locus_tag="PLS88_06085"
     CDS             14114..15256
                     /locus_tag="PLS88_06085"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOU06944.1"
                     /translation="MKRLLYFDKDYSRIRTIVRLFSKYFDISVAGDLLELSVMIERQK
                     PEILVAGLTSLDSAFNARQKESIKRITPPRLEIHYFHNYFELNDIIARVSEKASKDNL
                     LSCADKAGNAFGTKPQPADSSLIIIGKSNKLCYMISQIRMLANESCSVLIIGETGTGK
                     ELAARALHEWSERKCNPFLALNCAAIPNTLFESEMFGTERGAYTDASSRSGVIEQANN
                     GTLFLDEIGSLSPISQPCLLRVLDNGEYCRLGSPQTRSSKFRLVTASSRNPIDLATQQ
                     KFREDLLFRIANFVIETPPLRERKEDIPLLAQHFCDQYAKGRFNIGSDAFDKLYSHNW
                     PGNVRELCSVMMSACSLSKSDKICAKDIRFLPGIEKISERGKRQSR"
     gene            15319..16110
                     /locus_tag="PLS88_06090"
     CDS             15319..16110
                     /locus_tag="PLS88_06090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU06945.1"
                     /translation="MSNQNPEDIQGHLQIQNRTSTLSGKLALIVGGSGGIGLAISNEL
                     ASRGAALLIQGRHASDKLQENSFPKAKSLGLIDLEFSNPAQFIQALDLRLEDIASEPD
                     IVVCAFGPFLEKPLKACTTKDWEWLTLADLALPGALTSRFLPGMMERKYGRFIFFGGT
                     RTDTVRSYKKTAAYASAKTGLGVLAKSIAVEGAPNNVAAIVVCPGPVETEYLDRETRI
                     RHASLTATGSLSKADIIASIALNLIDAEPCIASGAVVNLDGGFAP"
BASE COUNT         4479 a         3963 c         3654 g         4077 t
ORIGIN      
        1 gagcgctcga tgaaactacc aaatctatcc atagttgtca agtgctctag aaggcccaga
       61 aagctttgca tatttcgcat ttatttgctt attattgcaa attcctgcac aacacgcagc
      121 attaagtgat gggcgcggat gtgaacataa ccgaactagc tcgagttggt caagaggtct
      181 cttggcaatt atataaaata aattctttgg tttttcgttc ttttacttat acaatttttt
      241 gaattgaact aaatagtcta caaaagacag aacagtggca atagctacaa caacatagga
      301 aatataagtc aaagtttgca aaacacttgc aagccctgcc atttcgctag aatgattcga
      361 aaagctgcca aaagcgacac cattcatgtt agaagcatag agctgaacag tatacaaacc
      421 caatgaaagc aagcccgcca ccatatagct accagccttt agcttgcccc ctggacgagc
      481 acccatggct gtccctttaa gcgctaacaa ggcacgtaaa aaaagaatcc cgaattcgcg
      541 atacataact attagaagaa cccacaaggg cattatgcct atgcttgcaa aggcgagaaa
      601 atatgtaacg cgggagatga catcggcaaa aggatcgaat agcttgccaa aagaatcaac
      661 atgattgctg cttctggcta tcttcccatc gatgagatcg cttatttcca tccaggcgaa
      721 tagtatccat agcacgataa tagctggctc tcgcggaatg aattcggctt taataaaaaa
      781 gaaaaaaaaa ggcgctagaa ttattctgct aagtgttatc ttatctgcaa ggctcatcga
      841 gataagcata tggactaggc atcgagcttg tcaagattat gctcaaggct atagtataat
      901 aatcaaattt cggcctgcgc ctaccaggtg aaaaaaaagt ggctatctta taggatataa
      961 atattcatgg aagaacgaga tagaatttct ggccttcgcc gaaacatcct gctcacgatg
     1021 tcgatattct tcgttgttca atgcgctatc tttttgacat ttgccttagt tgtggggttc
     1081 gaatcgcaat attggacgat atttctgcca ataagcactg gatttcatat ccttattctt
     1141 atattgttgc ttctttttca aaatgacttc tatatcgaat ccacgggtca aaaactcgat
     1201 cgaataaatc ttgccaatat aattacgctg agcagagtaa gctccatgcc tactctcctt
     1261 gtgctagtaa tagccgcaaa agaatacagt atacgattac cggtcttgat tttaattgct
     1321 cttatttttc ttaccgactt tttcgatggg cttatatcta gaaagacaaa tcaagtcaca
     1381 aaaataggta gaattatgga ctcggtcagc gattatgcgc tgcttattgt cctcagcatt
     1441 atttttcagt attatttact tattccggga tggctcttct ggctcattat agcgcgcttc
     1501 ggaattcagg ggctgcttat ggcagtgctt atgataatcg aaaaacgcat cgagccgaaa
     1561 tccacattta tgggaaaggt gaccattgca tccataatgg tgctttatac gcttgaaatc
     1621 ctaagagtga ttttgcaagc tccttcccat ttggcattcc ggatagcgga gtggatagtt
     1681 gctatcataa tcgtaattgg cattttcgat aaaatcgcca gcttttttga caccctcaag
     1741 agcaaaccct ccgcaccctg atttatctta ttctcaataa tcgaggagga attgcaatgg
     1801 ctgtcataaa aagtgcctta gagctcgcac tcgaaaggac aaaagatcta caactcgacg
     1861 aaaatgccca aaaaattgcc gatgcgaaga tcgagggtcg aaaagcggca agccgctatt
     1921 tggaagatcc agcttcggtc gatttcaagg caatattgag cacattagat cctgttcagc
     1981 gtcaagcttt tttgagttca gccttcgaag tactcagcaa ctttatccag ctaccaacca
     2041 attcagtggt cgataccgaa aaaatggaag ccattggcaa ggctatagtc ttactctgcg
     2101 gtctttctgc gcgctttccg tccgaaaaag aagtaaagct tgcgcaacaa caggctcgct
     2161 cgctttttca acaaatactt aggttcctaa gccaatatca agaggaaatg aagcgggtcg
     2221 aacaggcgat tcgcaatcag tgggcaccca agctaaaaga aagagagaaa caactagccg
     2281 cagcactcgg gcagaatgta cgaatggatc caatgtccga ccctgaattt gcggaattct
     2341 atcgaaagaa tatcgaatcg atgcggaata actatggcaa agctctggaa gatgccaagt
     2401 cgcagcttgc ggatatctgc ggtttcgagg cccaataatc atagaagcgc aatatcgaat
     2461 aaactagcgc tgaggcttgt tgagcgctat atataaaagc gcgatatcgg attttcggac
     2521 tcctggaatt ctcgaagcct gaccgagcgt gaggggctgg cttgagctta gcttctccaa
     2581 ggcttcttga gatagccctt ttactgcttt gtaatcaaat ccttgcggaa tgaacagctt
     2641 ttctgcacga gcaaggcgat gtgcaaggcg attctctttt tcctgatagc ccttgtagcg
     2701 ctcgttgaga agcgcggtta taacgcccga ggcttgcatg cccatactct cgggcagaat
     2761 cttggcaagg cgcgcaagag cctcggtttc gtcatcatag aggcaaccga cttttggatc
     2821 gcgaagcgca tcggaaagcg cctgaccaag gtgaccttcg agttcgggca tctgcgcaat
     2881 atcctcatga ctgattttat ggctttggag aagggtttct gcctcttcta ttgttcttag
     2941 cctgcgctca aagctttcgc gacgctgcaa atccacaagg ttcgtttcaa ttgcgtaagg
     3001 cgtgagtcgc aggtccgcgg tatcgtgcct aagagaaagg cggtattcag ccctgctcgt
     3061 aaacattcgg tagggctcct ttggcgagag agtcactaga tcatcgatta ggacgccaat
     3121 ataggcctcg tttcttccaa ggatgagtgg cgatgttcta tcgagtttcc tgcgcgcatt
     3181 gattccggcc ataagcccct gagcagcggc ttcttcgtac ccagaagttc cattggtctg
     3241 gcctgctata aaaagtcctt ctacaagctt agactcgagc gaagcataga gcgcagcggg
     3301 atcgatgtaa tcgtattcga ctgcataagc aggcctaacg attatcgcat tttcaagccc
     3361 aacaatgctg tgatagaaac gctcctgcac atcttctggc agcgaactag aaagcccatt
     3421 tagatacatc tcgtccgtgg aaagcccttc aggctctata aaagcctggt gtcgatctct
     3481 gtccgggaac ctcattactt tgtcttcgat agaggggcaa tatcttggcc cttttccgac
     3541 aatacgccct gaaaaaagtg gagagcgatc tagccctgag cggattgcct catgagtcgc
     3601 tgcatttgtg tatactacat agcatggaac atcgggcctc tcataatcat gctcaagaaa
     3661 ggaaaagaaa atcttggcct tatctccgcg ctgcacctcg agcttagaaa aatcgatgct
     3721 tcctcgcttt atgcgagccg gtgttcctgt cttcattcgc cctagaggaa agccctttgc
     3781 gcgaagcgcc gttcccaacc caattgccgc tggttctcca aggcgaccgc caggcccact
     3841 ccatgctcca atgaagagct ttgcttccat aaaagtgcct gtggtcagaa ccacaacccg
     3901 agccccgata gtgttccctc gctccgtaac cactccttcg atgcgcggat gggtaccttt
     3961 agataaaata aagtccacaa ccgtatccat aaaaatcgtt agatttggct ggttctcgag
     4021 agcttgtcgg gctagttttg catagagggc cttgtcggct tgcgcccttg gcgcctggac
     4081 cgccgcaccg cgtgagcgat tgagcattcg catctgtatg gctgctgcat ctgctaaaat
     4141 tcccatctgc ccaccgaggg catcgatttc gcgcacgaga ttgcctttgg aaagaccacc
     4201 tatggcggga ttgcaggaca ttctggctat ggtgtcgggg ttctgagtaa tgaggacggt
     4261 attggttcca agccttgcaa gggcgagggc agcctcgacg cccgcatgtc ctccgcctat
     4321 gacgatagcc tcaaaatctc ccatgtcaat ctctcctaat cttttgcgct ttttcggata
     4381 cgcgatttgg cgctttgcgc cggcgcgctg tcgtttttag cgttctagcg ctctggcact
     4441 ttcggcttag cgcgccggtg gcagcttggc gcgctggcgc ctcttcactt gccgagacaa
     4501 aaacagctga atatgcgctc cagcacctcg tcggagacta tgtcgcccgt tatctcccct
     4561 atggattctg ccgcttcttt aatatgcaga gagacaagat cgagtgctac accagcctca
     4621 taagcctcca tggcacgatt cagcgcttct tgcgcctcgg aaagaagctg cttttgcctt
     4681 tcgcttgcga ttctgacctg taattcctgg ttttgtggct caccgctttc ggacttttgg
     4741 tattgcggct cgctgctttg ggggatataa cctttatcga tcttggcgcg gagttcatct
     4801 accagcaccg attccaaaaa cccaaaggaa gcaacatcct tggcacttat agccttccag
     4861 cccgcagggg ccgacaggca atccatccga tctactttgt tccagagaaa aagagcccca
     4921 ggataagcgg ccataaagtt tttgtcctct tcataaagcc cgaccgttcc atctaccagg
     4981 tagaaaatga catcggccct atccagcata gccttggctc gacgcacgcc ttcaatttct
     5041 atctcattct ttgagtcacg aagaccggct gtgtccacaa gtctcaccgc tagcccacca
     5101 atcgttatcc aggactcgag ccaatctcgg gtcgtgcctg gctcttcgct cacaatggcg
     5161 cgctcttctc tcacgagcag gttaaagaga ctcgatttgc ccgcatttgg ccttccggct
     5221 atcaccacca atagaccttc ctgtcgtaat tttccgcctg gatagctttt aagaagctcg
     5281 gcgagagcac cttgaatctg gctgataatc ataggccaac gctcgatctc ttctggtcct
     5341 tcgtcttccg agtagtcgag tcgcgcatcg atatccgcaa gcacatctag catcatgctg
     5401 cggagctctt tgagtcgctt agaaagcaca ccagaaagcc gcgagagggc atcgtgccga
     5461 gcggcttcac aaaaagccag gctcaactca tttatggcct cagcacgaac caggtctgtc
     5521 ttgccttcaa taaaagcccg aaaagagaat tctccttgta aagcaggaga aaatccttgc
     5581 atataaagca aatccagaat ccggcgaacc actgcggccg agccatggca gagtatctca
     5641 accgcattct ctccagtaaa ggaatgcgga gccttataga caagggccat aacctcatcg
     5701 attcgctcat ttgagagagg gtcgattatc catccatgga ctgcctggta gccagcagcg
     5761 ccaagaagag cctcgggccg cgaaaagcaa cgagctacaa tatctattgc agcctcgcca
     5821 gaagtgcgga taatcgccag ggcggatcta ccctctggcg tggctagggc tactataggt
     5881 gtatcacggt caaaataatt tctagacata caaaactacg atagcctcac agcgcccttt
     5941 attacaagga gttcgggtgc ttattcaaag ccgaaggcca taaatactta cagtaaagcg
     6001 acgatttagg tacaatagag acgaggtaaa atacatgact ccacaggaac tatccagact
     6061 cgattatgaa ggcgcttctc agactatact cgcctataca atcgatgcaa aaaaatacga
     6121 aaaggaaatt gcagctctta aatcccaggc ccaagagtgg agagtcaagg ctgcagaagc
     6181 tcagaccaag gccgctcccg agctttccga ggccgcctct aaaagagcgt cggagttgga
     6241 atcgaaggcg cagcagcttt cggtagagct tgtcgctatc cgtcgcgaca tagaacaatt
     6301 gcgcgaagct ctccccataa tcaaggcaaa acagcgcagg gtcgatcccg accaactcct
     6361 tgcggagctc tcgatgatag tgggtttgga tgctctcgga gacaataaag agtctggtcc
     6421 caagagcgat tcgacaactt cgaacgttga taaggattcc ggcgcagtaa aggatcagga
     6481 cagttccgac aataaaatcc ctgcaagccc catgaacccc gtgagttcag ttaagcctgc
     6541 aagccccgta acccctacga gcccagcgaa ctccgagagc ttcgcgaacc ctgaaagtac
     6601 ccaaaatccc tcaaaagctg cgagcctagc cgaccaggcc ctcgacgatg ccctagctga
     6661 gcttaagaaa aaaatgggtc tcctctgact ttgtcttttt atttttatgt cgtattttaa
     6721 taaacaaata atatagagaa cagcaagatg aaaatgcgtc atttgcccgt attgcctgaa
     6781 ttccctcggc cgcttctttt tgcacatcga gggctcaacc gaaaatatct cgaaaattca
     6841 atggaatctt tcgaggctgc atttaatgcc ggagtgcccg gcatagagct cgatgtccat
     6901 ctgacagccg atgataaact agtgattttt cacgatgaca caaccagccg tatagaacaa
     6961 aaaattcaac ccgaagctcc tgcccgcaac cttgagctcg aggcttccac ttttgtcgaa
     7021 ctccgatctc tctttattgg tgctcatatt tctcttctcg atgagctttt tgactgcttt
     7081 ggagaagcgc tatattacga tatcgaactg aaatctcgca gcacggcaga tactggactt
     7141 gcttcgaagg ttgcgagtgc gatccgttca cacaatctcg aaagcaattg cattatctcg
     7201 tcttttaatc catttgctct gcgctacttc cgacaggcgg aatcggctgt tccagtaggc
     7261 ataatctgga gcaattcaga cgagctctat tggtttctgc ggcatgggga aggtgcactt
     7321 atttcgaaat cggattttct aaaacctgag tcgagcttgt ttgcaaacat ggctggccgt
     7381 ccaccgcttc atttgcttcc atggttcaga ggctcaaaag aaaagcctat aattccctgg
     7441 acggtaaacg atccggaaaa agcctgctat ttcattgacc aaggctcgac aggagttata
     7501 tcggacatcg cggacgaaat acttattcga tttcgtgcag agcacgagca agcggcagcc
     7561 gccgacccaa gccaaaagct ctctttgaaa gcttaattgc aggtgcggct tgcctgcgct
     7621 tgtattccgc tttgccggtc atctccagcg tttttcgcac ggtgccgcga tcgaatccta
     7681 agtttacgat ttggtccata gagagatttt cttctatata taattgcaag attttgtcca
     7741 gcacttcgta ttctggaaga gtatcctgat ccttctgatt atagcgcagt tctgcgcttg
     7801 gggctttggc aataatgggc tcaggaatgg gttcgatatc gccattttcg gctgctactc
     7861 gattgatata gagagcgagc ccatagactt cggttttata aaggtcggcg ataggagcaa
     7921 gggagccgtt catgtcgcca taaagggtac aatagcctgt ggcaagttcg ctcttgttgc
     7981 ctgcggtgag cagaagtgca ttgaatttat tagaccaggc catcagcagg gtccctcgaa
     8041 tgcgcgcctg cagattctct tcggtggtat cataaggctt accagcaaaa ggcacggaaa
     8101 gcgcctcgag gtaagcggta aagggaacct ctatcggtat tcgctcgagt ctaagcttat
     8161 tgcgtctgca gagctcgaca gcatcgtcca aagatgcctc gcttgagaat cggcttggca
     8221 ttgcaatgca agtgacattc tgccgaccta tagcttgtgc ggccagcaca gctaccagtg
     8281 ccgaatcgat acctccggaa aggcctaaaa ccaccttaga aaatccggat tttctcagat
     8341 aatccttaat tccaactaca atcgctttct gaatttcctc gtacctatct gggcaaggaa
     8401 gttgattctc ttcgacaaac tctgccttct gaagcaaagg atcgaagagc atgcgattcg
     8461 ctttgccgct gacactgtcg tagataagcg cttcttcagc ccatccgcat attccgcaaa
     8521 tggaagcatc ttctcccata gcaaaagagc gcccatcgaa taccaatgag tcgtttgctc
     8581 cgactgcatt acagtagagc actggaatgt atcctgcgcg aaccgctttt tcagcaagtc
     8641 tgatccttgt ctcgagtttt ccagcaataa aaggcgaagc tgaagtgaca actattatat
     8701 cggccccttc gtccataagt tcctttactg gatcgatcgc atattcttgc gaggcattgg
     8761 gttcttccca ccaaaagtcc tcgcagatag caaaaccgac cttaccccat ggttggacaa
     8821 agaggtttcg ttctttggct ggttcgaagt accgagcttc atcgaaaaca tcataggtag
     8881 gcagaagggt cttagcttgg gtaaaaacca ccttcccatc ttggataatc gaatatgcat
     8941 ttacgagcga ttttccagtt ccattacgat ttctgtcaac gtaacccact ccaactgcca
     9001 catccgaagg caacatcttc tgaagcattc gaatagccgc tatggaaccc agcaagaaag
     9061 attcctgatc gagaagatcc atcggcgggt acccgcatag acatagctca ggaaagacca
     9121 caaggtcgac tctttccaat gcaggaaaca tggaaagcat gcgattcacc atggcgatca
     9181 ctcgatcggc attgcctttg aagtctccta ttttggaatt gagctgaatt agagagattt
     9241 tcatcgctct cccccgcttt tgatcttctt tcgcagctta cgagcgctag aggcatcata
     9301 tttcgtggat ctggcattac cgctgccact tgctcgacta gcgcctcctc cttttctagc
     9361 ctgagccgcg ttaacctctc ccccagctct cgatgcatct atgaatatag gctctcctgg
     9421 ttctatctta aaatgctttt ttgcatagaa aagaaatatt agagccccga tgatcatgat
     9481 aaaacttaga atctggccca tcgaaaaatt gaaaggagag acaaagagcg ccatgggtgc
     9541 atccggatct ccgagagcta aaatatagcc taatcccgaa tcgggctctc ggaagtactc
     9601 tacaaagaag cgtgctattc catatcccag agaatatgct gccacacctt cgcctcgaaa
     9661 acttcgccga ggacgcacga aaaaccatag tattagccat aacacgatgc cctcgagcag
     9721 ggcttcgtaa agctgggaag gatgacgcgg cagatttacc atatcattca tggacacaat
     9781 tcgaattccc actttgcgcg ctatttcctg aacccattgc tccttagcag aaaaacgctc
     9841 cgcattagga aaaaccatgg cccaaggcgc ggtgctgacc tttccccata gttcgccatt
     9901 tataaaattg ccaagcctgc caaaagtata gccaagaggt gccgaaaccg cgatcaagtc
     9961 cgcccaatcg aaccaattcc atttgtggac cttgcaccat attagagtgg caacgataag
    10021 cccaacaaag ccgccatggt aggacatacc ctgaaaacca acaaatgtcc ctgattcatc
    10081 gaaaggccaa aaaatgtacc atggtttgat ccaatagtaa ttcgttggct cgtatattag
    10141 tgtgccagcc aacctaccac caaggagaat cccgacgatc ccccagatga aaaagtttgc
    10201 tgcttcgtct tcggtccacg gagcaccgag ccgtttggat tctcgcttaa agagaagcca
    10261 tgtaacgcca aatgcaacga gatacattaa gccgtaccat ctaaagggca ggcctggaat
    10321 tatctctgga gaaagccact ttggaaattc aagcatcgca accatcagga gacctcatgc
    10381 tgatctttat gagtcttgtc cataatctct gtcttttcca tgttctctgg attttctgcc
    10441 ttttttgcct ctggcagttt aaacttaaga gcatctatca caagcaggcc caaaacaaca
    10501 atatccagca aaatcaaggt aatcatcgag gtagcagcct ttgccatatc cacaggaaag
    10561 gacccgccaa tattgcgaat gtaggtcaac aaagcaagca cactaagtat cggagaaaca
    10621 caagccacga taagcgaagg cttcctaaga tattctcgcg ctcctgcctt gatatactga
    10681 attagaacta tgtagacgaa taagagatta tacaactgaa atattcgaag caacgccgat
    10741 gttacgccac cataatcgct cattccaaac agaatcgcca caataccaat tataaagata
    10801 cggagaaaag ccacaattgc ggcaattagc agaaaaagcg aacgttgcat atcgaaaagg
    10861 agtctattag gcctatcttc catagtcaag acagtagcaa aattatggtt aattggctat
    10921 gataatgtaa ctcatgagaa taccagtctc gggaaaaaag gagtctaacc cgatgaagca
    10981 tatagatgtg ccggcaataa gaaagcagat aatttctttt gttagccttg ccgttgtgct
    11041 ggccttatca ttcgtaacag tttcggttcc ggagctctat gcacaacagc taaaaactcc
    11101 tgattcaaag cagtattcat tgctgctgca gaacatctat cattttatcc ttcagaacta
    11161 cgtcgaggaa ccagacccca ccaagctcta tgagggagcc gtaaagggta tgctcgactc
    11221 tctcaacgac ccatactcca catatctaga tgaggacatg atgtccgatc tgatgtccga
    11281 cactactggt atctatggag gaataggtct ttatatctcc aaacaagcaa cagtggcaaa
    11341 cgagaacgct ccacgatata tcgaagtcgt atcgccaata gaggatacac ctgcttggag
    11401 agaaggaatt cgccctggcg atctaattat aaaaatcgat ggcgaagaca catctccttt
    11461 gagcaccgac aaggcctcgg ccaagatccg aggagaagca ggcacaaccg taactctcac
    11521 atttagaagg ggaacctacg agttcgaaat aacatttact cggaccaaga tagaaatccc
    11581 tgcgctaaag agcgcgctca tccagacagc aaatggaaat gtcggttata tccgcataat
    11641 cgagtggaat gccaatactg cgccgcagat gaagacagtg ctccaagata tgaaaaaaca
    11701 atccattgac aaattcattt tagacgtgcg ctcgaaccca ggcgggctct tgtcttccgt
    11761 ggtagatgtc agcgatctct ttctatctac gaatgtaatc gtatctacaa aagggcgcag
    11821 ctcttccgaa aattatgagt acaaggcaaa gccagaccta gcaattcctg caactgaccg
    11881 gatgatcgtc ctcatagacc aaggttctgc ttcggcctcg gaaatctttg ctggtgccat
    11941 gaaggataca aagcgagccc tccttgttgg agaaaaaacc tatggaaaag gatcagtcca
    12001 gcagatattc cctctcgata aagggggctt taagcttacg atggcgcatt actatacacc
    12061 aagcggcatc gatatcgaca aggtcggaat cgagccggat gtaaaagtac ccgaacctac
    12121 ccttaacgac aaggaactcg aggaacttca gcgcctttac gacgcaggag atatagctcg
    12181 atatctcgag agcaatcctc agccaactct tgccgatcgc aaggctttcg cagcggaact
    12241 tgcacagaaa tataaaattc aggagatttt tatcgaaaag ataatccgcg atgaggctga
    12301 gaggtctatg ccagcccgaa tctatgacct cgaatacgac atacagctgc aaaaggctct
    12361 cgattttatc gaatcggcag atttcgaaca gatgttggag aacaccaaga ctctggctca
    12421 agtccgcgcc ttgagcaatt agataaggct tgctcttatg cgccagattg ttctattagg
    12481 taaccagcgc ggccgagaaa cttgctctct cgacgaaaaa acaagccatt acctaataaa
    12541 tgtccggcga atgcgaaaag aagactcttt taatgcagtg gatgaggcgg gttcgcgttt
    12601 tcgctgtatc atcatctcgg acgatccgcg cgcagtaaag ctgggaatta caccgatttc
    12661 ttcgccccga tccttggtga gcagcgcttc aaatacggca gaagaaggcc aaccaaaaat
    12721 cggcatagct ccaactctgc atatcgcttt agtccaggct ctacccaaag gcaataagtt
    12781 cgatctcatt attcgccaag cagtggagct ggaggtcgaa ctcattattc ctttgattac
    12841 tcaacattgt gttgaacagg agccatctga gcgagcaaat gccaagctag agcgccgaaa
    12901 gcgcataatc cgcgaggctg ttcagcagtc gggctctctt gtctacacag agattctgtc
    12961 cactgcaaac ctagaaacgc tggatgcggt cttgaacgca aagggtttta gtaaaaaaga
    13021 ttcggtaaga attctatttc atgaatcaga gcaagaaaaa tcgcaatcgc tgcatcttct
    13081 actttcgggt gacaagaata gcgttgtcct tgccatagga ccggaaggtg gtctttctcc
    13141 aaatgattgt gcggcattta tcgatatggg ctttgctgtg catcatgcga gagggccggt
    13201 gttgagagtg gaaaccgctg ccatttttgc aatatcggca gttagagcta tcgctttcga
    13261 gagaggctta tggaaagtgt aagcatagcc tgtgcatttc tagatggcct gcgtctcgat
    13321 tttccctcgc aattcgacct gtttacattg ccctatctat gggacgatga aaagaatcac
    13381 cagatttgtt ttgtcgataa acagagctgg atacatgcca aaaagcgcga cgaatttggc
    13441 tccatgatag ccagcgccga tatgatttta aatcgcgata gcggcgctgc ggaaagtgtg
    13501 ctcatgcgtg gcgaaaaaga ttctcgctgt tggcctgtac cttttattca tcaggtattg
    13561 ttggatgcta taaaagaatc catggaggaa gaaaattctg ccaggttaga aaacccagga
    13621 gaagaaacca aagcctatag accccaaaag gtgctaaccc tcctgctatc gtctctcgaa
    13681 aagcgaaatg gctccgtgtt cttattggga ggaaacccga taactcttat aaaggccgaa
    13741 aaaaacatcc gcgcaaccta cccgggaata acgatagtag gaagcatgca tggcctatat
    13801 cggccagagg aagaatctgc tataattgag gccatacaaa agggagctcc tcaactgatt
    13861 ctggcgggta gtccgctccc tgacggagaa tgctggatac cccgtcacat gaatataacc
    13921 agaagcggta ttttctttta ttataagcct ataatgaaat ggatttccgg cagatgaaat
    13981 ctcttttttc tctagactca attagacttt agctaacagt tgagagatat atcgggtaaa
    14041 aatatcttgc atagaccaag ctattcccca tgtcttgaaa catgaatatg cgtttgttac
    14101 atcttaagca atcatgaaaa ggcttctcta ttttgataag gattatagca gaatccggac
    14161 aatagtaagg ctttttagca aatatttcga tatatctgtc gctggagatt tgctcgaatt
    14221 atcggtaatg atagaaaggc agaagcctga aattcttgtt gctgggctta catcattaga
    14281 ttcagccttc aatgcacgtc aaaaagaaag cataaagcga atcactcctc ctcgattgga
    14341 gatacattat tttcataatt acttcgaact caatgatata attgccagag tctcggaaaa
    14401 agcaagtaaa gacaatctgc tttcctgtgc tgacaaagct gggaatgcat tcggaacaaa
    14461 acctcaacct gctgattctt ccttaattat aattgggaaa agcaataagc tatgttatat
    14521 gatttcgcaa attcgtatgt tggccaacga atcttgctct gttcttataa ttggagaaac
    14581 gggaacggga aaagaacttg ccgcgcgagc cctgcatgag tggagcgaga gaaagtgtaa
    14641 tccttttctg gccttgaatt gcgcggcaat tccgaacact ctatttgaga gtgagatgtt
    14701 tggcacagaa agaggagcct ataccgatgc ttcctcaaga agcggggtca tcgagcaggc
    14761 aaataatggt acgctctttt tagacgaaat tggaagcctt tctcctattt cccagccatg
    14821 tctgctgcgc gtgctcgata atggagaata ttgcagactc ggaagccccc aaacccgctc
    14881 gtctaaattt cggcttgtta cagcctcgag ccgaaaccca atcgacttgg caacccagca
    14941 aaagtttcga gaagatcttt tattccgcat tgccaatttc gtaatagaaa ctcctccctt
    15001 gcgcgagcga aaagaagata tccctttgct tgctcagcat ttttgcgatc aatatgcaaa
    15061 aggtcgcttc aatataggaa gcgacgcctt tgacaaactg tattcacaca actggcctgg
    15121 caatgttcga gagctttgtt cggtcatgat gagcgcatgc tcgcttagca agtcggacaa
    15181 aatttgtgcc aaggacataa ggtttttgcc tggcatcgaa aagatatcag agcgaggcaa
    15241 gcgtcaaagc cgctaaagag agaatcttgg ctaaaatcgt cgctatctaa caagtaaaaa
    15301 ttctagtatc ctaaagccat gagcaatcaa aatccagaag atatccaagg ccacctccag
    15361 atacagaatc gcacatcgac attaagcgga aaactcgcgc ttatcgtagg cggctcaggg
    15421 ggcatcggtc ttgccatatc caacgagctt gcaagcagag gcgcagcgct gcttatacaa
    15481 gggcgtcatg cttcggataa actgcaagaa aacagctttc ctaaggctaa gagcctcggc
    15541 ttgatagact tggagttttc gaaccccgcc caattcattc aggctttaga tttgcgtctt
    15601 gaggatatag caagcgagcc cgatatagtc gtctgcgcct ttggtccatt cctagaaaaa
    15661 ccactaaaag cttgtaccac aaaagattgg gaatggctaa cgcttgccga tttggcactt
    15721 ccaggagccc tgacaagccg atttttgcct ggcatgatgg aaagaaaata tggccgtttt
    15781 atattctttg gcggtacaag aacagatact gtccgctcct ataagaagac agctgcttat
    15841 gcctccgcaa agactgggct tggtgtgctg gcaaaatcca tcgccgtgga aggagctccc
    15901 aacaatgtcg cagccatcgt cgtgtgccct ggtccggttg agaccgaata tctcgataga
    15961 gaaactagaa ttcgccatgc gtcgctgaca gccaccggtt cactttccaa agctgatata
    16021 atagcgagca tcgctctcaa tcttatcgat gccgagccat gcatcgcctc tggagcagtg
    16081 gtcaatctag atggaggttt tgcgccataa cacttttgtg ttagcgggtg tttgagggtg
    16141 cacgacaatt ctgtgtaaag ctagttaggc agc
//
LOCUS       DAOSOB010000047        16043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3680_length_16043_cov_71.707093, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000047 DAOSOB010000000
VERSION     DAOSOB010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 16043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16043
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3680_length_16043_cov_71.707093"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /gene="ptsP"
                     /locus_tag="PLS88_06095"
     CDS             complement(<1..1381)
                     /gene="ptsP"
                     /locus_tag="PLS88_06095"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOU06946.1"
                     /translation="MTIIKGIGVSPGVAIAPAFVYIDDSGIIPQYHISAEDVQSEMAR
                     FHEASLLAKKEIEALRDRAKKEAGEEQAAIFDAHIMMLDDPVLLEQIELSLKEKQFNV
                     EAAVLSFESEMVNKLSSSQDPLKQEKVSDIHDVVRRLLGHLLKKERISLSDIQTEIIL
                     IAKDLLPSEMVMMSRSMVRGIVTETGSRTSHAAILARAFEIPAVLGVGPGFVQQVKQG
                     QAVYVDGDSGTVVIEPDDSIIRSEKAARAARLKQEKENKELRDIPAHTKDGTGIMLLA
                     NIGMPEEVTNAIEYGAEGIGLFRSEFFFLGGHIPGEEEQYNAYAQVARSMNGKPVTIR
                     TLDIGGDKVLPELGVMGEKNPLLGWRAIRFCLEKTDLFITQLRAILRASDQGKVNIMF
                     PMISTIDELIRAQALLDKAKEECISQGYKIDEDIETGIMIEVPSAAICSDVLSRSCDF
                     FSIGTNDLSQ"
     gene            complement(1470..2309)
                     /locus_tag="PLS88_06100"
     CDS             complement(1470..2309)
                     /locus_tag="PLS88_06100"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU06947.1"
                     /translation="MLLGSSFRPAPGPMTARGGSDVSDFAKSNEIVMPEPEDKLVKYS
                     VYEVKKGDTLSEIAEHFDLTLDTLISINNFKSAKSLKPGQLLKIPSESGIIYTASKPT
                     TIQEIAESYNINIDRLIDTNGLLSETIPAGKPLFLPDVKLPAATLREIAGTLFQWPVR
                     GRLTSWFGWRKDPFTGRRSFHNAVDIAAPYGSPIAAPMEGKVVETGYSPILGKYVMMS
                     HSGGWKTLYGHMSEILVQEGQYISQGRTLGRIGTTGYSTGPHVHFEVIKNGVLVNPLN
                     YLP"
     gene            2541..3707
                     /locus_tag="PLS88_06105"
     CDS             2541..3707
                     /locus_tag="PLS88_06105"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU06948.1"
                     /translation="MNSERADVALIRCDDYESKKLDRAIETCAEIAGFPDIKDKIILI
                     KPNVLNASPASKAVTTNPAFVEAVVRFVKSRGAKEILVGDSPGWQPVLLAAKTSGIYD
                     AVQQSGGTWVDFREVSPHPVVDGKKLKNIPLTALLEKVDLVINLPKLKNHSLTTYTGA
                     IKNLFGLIPGTAKSAMHLQYPAVIDFGEMLVDLALSIPNCFTFMDAIIAMDGQGPGSG
                     TPYSLGIVLASMSVAKLDWIASQCVGYDPKNISYILDSMQRTLGSANVPEPSTYPLRV
                     SEVEHSGFKRLPYTSKLGKRLSSIPRGLRSFAAKFIHLRPIFHKNKCIGCSACITICP
                     AKVLVLDRKNGSNIIRINDKLCITCFCCHEVCPAKAISVGRAPIRIVHRRLPVN"
     gene            3757..5361
                     /locus_tag="PLS88_06110"
     CDS             3757..5361
                     /locus_tag="PLS88_06110"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOU06949.1"
                     /translation="MAHFSLDDLIHFGNLSDFEIMDLPAPMEKNGHLVLLADAIRECA
                     RIAFTKIAYLMPKTQYDAFVNIIEDPAASERERFVAACLLENALISGKGRYPICQDTG
                     TALVYGWRGNQVEIEENIGLEELLAQGSAEAWLQEKLRNSQMGSFNMIKECNTKDNLP
                     AGIQIRSIKGKMLKLSFVAKGGGSASRTSLSIESPAILREDKLEKVLQSRIKALGPSG
                     CPPYVIAMVLGGTNPSQSLYAAELAAIGLLDELPISAQGDGMPIRDILWEQKMLTIAK
                     DSGFGAQWGGSHFAVSTRAVRLSRHAANLPLAIAVSCAANRHVSVFIDNSGWHIEKLS
                     SPSQCLEKKVQDIIQRTTAKTEIIEISQVDNGWLSRLRSLQAGSMALISGPVLLARDM
                     AHARLLCMLELDKKLPDWFIGLPVFYAGPTEAKPGAATGAFGPTSASRMDSYLEAFMK
                     AGASIVTIAKGSRSDEARRAISTHRGVYCAAIGGVAALNAEHHLRTLDTIQFEDLGME
                     AVRIAVLDRLPIIVAIDSHGNDIYNL"
     gene            complement(5368..6045)
                     /locus_tag="PLS88_06115"
     CDS             complement(5368..6045)
                     /locus_tag="PLS88_06115"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOU06950.1"
                     /translation="MSKSGHFSVRSHFNTLKSLPIFAIENRMLAENDMVAGDGNGGRR
                     WMEISTRKVYECNVFSVLERESQGPDGRIGHFSLMRAQDWAVIVPYVRTDAGTSFLMV
                     RQYRHGAEELSLEFPGGVVEYGEEPSHAAARELAEETGWVSDRILHAGTVFPNPAIQD
                     NHFHVFVALDPKPAVDQHLDQNEIIDAYLMPADEVRSRIGEGELRHALMVTALYLADR
                     LVASIKA"
     gene            6246..7085
                     /locus_tag="PLS88_06120"
     CDS             6246..7085
                     /locus_tag="PLS88_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOU06951.1"
                     /translation="MEKLIMQSHRPLVIAHRGFRSIAPENTLLAAQKGFDAGADMWEL
                     DVAASSDGELVVLHDNTLTRTTDAKTRFPSRSPWTVYDFTLAEIKSLDAGSWYAQTDP
                     FKQIAIGRVKTSEIASFKDLKIPTLREALELTKRLGWRVNIEIKDATGFACDAWIVER
                     TAELIKELEMVPSVLISSFNHDYLKRMKKAIPEIAVAALVDRPLSNPVERLKDIGAVA
                     LNPNEKYLDEATVKALRSAGFGVLPWTVNDASRMNTLIDWGATGLITDFPDKALELVN
                     KTF"
     gene            7200..8501
                     /gene="ugpB"
                     /locus_tag="PLS88_06125"
     CDS             7200..8501
                     /gene="ugpB"
                     /locus_tag="PLS88_06125"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HOU06952.1"
                     /translation="MKRIVLLIAVLLMAVGAYAQTTVEFWHAMAGHNGEMVQIISDQF
                     NASQKEFKVVPVYKGSYVDTMNAGIAAFRAGQAPAIIQVYEVGTATMMAAKNAVKPVY
                     QLMAENNMKWSSDIYIPAIKSYYSTSDGRMISMPFNSSTAVMYYNKDAFRKAGLDPEK
                     PPVTWPEFFEVAKKLKASGMEGGFTTNWISWIQLENFCSWHNIPFGTRSNGFDGLDTQ
                     LVFNSPVVVRHFQNIYDLSKTGVFIYGGRENKANPLFTSGRVGLHFESIGGYGNMKAT
                     CKFDFGVARLPYYPDVPGAPQNSCIGGASLWVFNGRPAAEYKGVAEFFNFISKPEIQA
                     LWHKETGYLPVTQAAYELTKSQGYYVTNPGLEVAIKQLTNKTPTPNSMGIRFGNMPII
                     REIEDQVWEEILSGKISVKDGLDKMVREGNKQLREFERLNK"
     gene            8681..9565
                     /locus_tag="PLS88_06130"
     CDS             8681..9565
                     /locus_tag="PLS88_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU06953.1"
                     /translation="MSKQYEFKASILPYLLVLPQMVIVFLFFFWPSAQAILQSFYLQD
                     PFGGKMIFVGFENYSKLLVDPDYWQSFWVSTAFALFITLSSMVISLFLAIQANKKIRF
                     ASFYKTMLIWPYAVSTLVAGVIWLFMFNPNVGVIAYVLKHTFNVNWQYLLNFNQAFLL
                     LTMAATWKQLAYNFVFFLAGLQSVPATLVEAAEIDGAGPTKRFWKIIFPLLSPTTFYL
                     LVMDLVYGFFETFPIVHSITSGAPGKSTAILVYKVWRDGVINLDLGSSSAQSVILMIM
                     VIILTVFQFRFIERRVTY"
     gene            9582..10457
                     /gene="ugpE"
                     /locus_tag="PLS88_06135"
     CDS             9582..10457
                     /gene="ugpE"
                     /locus_tag="PLS88_06135"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HOU06954.1"
                     /translation="MKEQQRINKAAHRAKIIRSFYPHIILWIGIAIIIFPIYFIFSAS
                     SHTNSDIQAAPMPLGIGSHLIENYRQALLHGTQNMGTPVSTMLKNSLIMALGISIGKI
                     IISLMAAFAIVFFDFPLRGFCFWSIFITLMLPVEVRIMPTYKVVADLGLLNSYAGLIL
                     PMIVSATAVFLFRQFFMSVPREIAEASQIDGATPMQFFRHILAPMTRTPIASMFVIQF
                     IYGWNQYLWPLLITTKPEFYTLLIGINRMMSGADVQIEWQIVMATTMLAMLPPVIVVV
                     LMQKQFISGMTETEK"
     gene            complement(10587..12026)
                     /gene="uxaC"
                     /locus_tag="PLS88_06140"
     CDS             complement(10587..12026)
                     /gene="uxaC"
                     /locus_tag="PLS88_06140"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOU06955.1"
                     /translation="MAEFLDEDFLLKNETAKRLFFEIAKDLPIFDYHCHLSPKAIAED
                     KPFDDIAKIWLEGDHYKWRIMRANGAPESLCAGAGAWDEKFLAYANALTKAAGNPLVQ
                     WSHLELKRIFGIDEVLTPQNALSIREKANFILAEKRMSPRSLLERFKVSVLCTTDDPV
                     DNLAWHRAIAEEQRVGSSFTTKVLPAFRPDKAMKTEDISAWNTYIDLLGEAAGIEIES
                     FASLADALASRHAYFHSMGSRLSDHALLIPPFMQASDAELDSIVRKGRSGEDLSPIER
                     EKLDTALLLHFGRLNAQKGWTMQLHIGALRNVNPGLYRAFGPDVGGDCSSDAPIIGAL
                     ASLLGALEREGNLPKTILYTLDPSKHNPLATLAGSFAGTAPEISSPQVAGKVQLGAAW
                     WFNDQKDGMERHMREYAGVGLLGAWVGMLTDSRSFMSFPRHEYFRRLLCNIVGEWVEA
                     GELPDNPLYTEQLVRGICWENARCYFGML"
     gene            complement(12097..12924)
                     /locus_tag="PLS88_06145"
     CDS             complement(12097..12924)
                     /locus_tag="PLS88_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU06956.1"
                     /translation="MSFVESMFSLEGKIAVVTGGGGMLPSYIAIAFAKANAKVALWGS
                     GTHHPMEEAAKTVRKQADLPLDSADIIGITVDTGSTEACEQALEQTIQSLGVPDILLN
                     GVGGNIGRSKFVELDEKLFEEVLRLNLMAGLVIPSKVFAKRWISEGIKGSMINMTSMT
                     SYKPLSDTWAYDAAKAGVLNLTQALAKEFAPYGIRVNAIAPGFFVGNQNRALLIDEKT
                     GDLTPRGRAIIARTPFGRFGEYEELWGTALYLASSKASGFVTGVSIPIDGGFLIDNI"
     gene            complement(12980..14281)
                     /locus_tag="PLS88_06150"
     CDS             complement(12980..14281)
                     /locus_tag="PLS88_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOU06957.1"
                     /translation="MNVTIPTIILLGGFVLLLLLRVPITFSLATASIATAFYLHIPLM
                     AIVQQMVQGVKSFSLLAIPFFIIAGEMMARGGIAEKIINLANVLVGRVRGGLGMVNVV
                     DSMLFGGISGSAVADVASTGVIIIPLMNKNGYDNDFSVAITVTSATQGIIIPPSHNMI
                     IYSVAAGGVSVGRLFLGGYIPGILLGISLAIIVYILSVKRNYPKGRKYSFKEGIKIAI
                     DAFWGLFVAVIIIVGVVAGVFTATESAAVAAVYAFIITFFVYKQIPLREFRGILYSTL
                     KTLTMVMTLIATASSFSWLMAYLRIPAMATEFLLSITDNKYMLLFLVNVLLLFLGMIM
                     DMAPLILIVTPILYPVLIGKFGMSPIHFGIMLMINLAIGLCTPPVGSALFVGCAIGKI
                     SIEKASKAMIPFYIAMIATLFLVTYVPEITLFLPNLIMPGQ"
     gene            complement(14286..14825)
                     /locus_tag="PLS88_06155"
     CDS             complement(14286..14825)
                     /locus_tag="PLS88_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOU06958.1"
                     /translation="MKKFIVSAVTFIHILLINLSMIMIVAMLLLVFINVVLRYVFNSG
                     IYWSEEVALVLEVWFIFLSLGLGVKYRLHFSINIVNREKISASLNRALDMLTDIIYIF
                     IGAIMIIYGSKLVKFTMRSIMPATMLPAGILYLVLPLSGFVAIVEAIFHIFGFNMFDE
                     KLEAYFSGQGGRIRDILRS"
     gene            complement(14952..15929)
                     /locus_tag="PLS88_06160"
     CDS             complement(14952..15929)
                     /locus_tag="PLS88_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOU06959.1"
                     /translation="MKKFRIIALALLLVLLPSMLFAQAKPIVLRLAETHPKGYPTELG
                     DEEFARLVKERSNGRIIVEVYPGSQLGEEKAVIEQVQFGAIDITRVSISPVAAFVPKL
                     NAFQMPYLYRDSDHMWKVLKGDIGMELLASLEPFGFIGLGWFEAGARSFYNSKKPVYT
                     PADLKGMKIRVQESELMLGLVRSFGAIPTPMPYGEVYSGLQTGVVDGAENNPPSYYSA
                     SHYEVAKYYTLDEHTMVPEIIIGSKISLGRLSKADQDLIKKAAMDAIDYQRAQWDAYV
                     KVSMDKVKEAGCTIIPIPDKTEWMKAVEPMYKQQPKEIQDLVARIRAVK"
BASE COUNT         4437 a         3746 c         3433 g         4427 t
ORIGIN      
        1 attgacttag atcgtttgtt cctatcgaga agaaatcaca agatcttgag agtacatcag
       61 aacagatagc tgcagatggt acctcgatca tgattccagt ctctatgtct tcatctatct
      121 tataaccctg cgatatgcat tcttccttcg ccttgtcgag aagagcttga gcacggatca
      181 attcatctat tgtgctaatc atagggaaca tgatattcac tttcccctga tctgaggcac
      241 gaagaatagc tctgagctgt gttataaaga gatcggtctt ttcaagacaa aaacgtatag
      301 ccctccatcc cagcagagga tttttttcgc ccataactcc aagttctggc aatactttgt
      361 ctccaccaat atcgagcgta cgtatagtaa cgggcttacc attcatggat cttgccacct
      421 gggcataagc attatactgc tcttcttctc ctggtatatg accaccaaga aagaaaaatt
      481 cagagcgaaa cagtccaatt ccctcagcgc catattcaat agcgtttgtg acttcttcag
      541 gcattccgat gttagctagg agcatgatgc ccgtaccatc tttcgtgtgc gctgggatat
      601 ctcttagttc tttattctct ttttcctgct tgagacgagc agcgcgcgct gctttctcgc
      661 tccgaataat cgaatcgtcg ggctcaataa caacggttcc actgtctcca tcgacataga
      721 ccgcttggcc ttgttttact tgctggacaa agcctggacc tacacctagt acggcgggta
      781 tttcgaaggc tcttgcaagg atagccgcat gactagtcct gcttcctgtc tctgttacta
      841 tgccgcgaac catgctgcgc gacatcataa ccatttccga aggcaaaagg tccttggcaa
      901 tgagaattat ctcggtttgg atatcggaaa gcgaaatcct ctcttttttt agaagatgac
      961 ctaacaagcg ccttacgaca tcgtgaatat cggatacttt ttcctgttta agtggatctt
     1021 gcgatgaaga aagcttattg accatctcac tctcaaaaga gagaaccgct gcttctacat
     1081 tgaactgctt ttctttaagc gatagctcta tttgctcaag cagtactgga tcgtccagca
     1141 tcataatatg cgcatcgaaa atggctgctt gttcttcgcc cgcttctttc tttgctctat
     1201 ctctcagcgc ttctatttcc tttttagcca gcaaagatgc ttcatggaaa cgagccattt
     1261 cactctgcac atcctcggcg gatatatggt actgaggtat tatgccagaa tcatctatat
     1321 acacaaaggc aggagcgatt gccacgccag gagaaacacc tattcctttt attatcgtca
     1381 tgatgaaaca atgtatcaga tatttctata tgcgtcaaat aagatgctcc caaggcttcg
     1441 atgtgtctca tttcgagatc atgaggatat caaggcaggt aattcagcgg attgactaga
     1501 accccattct ttataacctc aaagtgcaca tgaggacctg tactatatcc agttgtacca
     1561 atcctaccaa gagtccgacc ttgagagata tactggcctt cctgaaccag aatttcggac
     1621 atatggccat ataatgtctt ccacccgcct gaatgggaca tcattacata tttgccaaga
     1681 attggcgaat agcctgtttc gaccacctta ccttccatcg gagcagctat tggcgagcca
     1741 tagggcgctg ctatatcgac agcgttgtgg aaagatctcc ttcctgtaaa gggatctttg
     1801 cgccagccaa accaggaggt gagacgacct ctcactggcc attggaagag cgtcccagca
     1861 atttctcgca atgtggcggc cggtagtttg acatccggaa gaaatagagg cttacctgcc
     1921 ggaatcgtct cagaaagcag gccatttgta tctataagac gatcgatatt gatattatat
     1981 gattccgcaa tttcctgaat tgttgtcggc tttgaggcag tatatataat gccagattca
     2041 ctcggtatct tgagtagctg accaggtttt agtgatttgg ctgatttaaa attatttatg
     2101 ctgataagtg tatctagagt cagatcaaaa tgctctgcta tctcagagag tgtatcgcct
     2161 ttcttgactt catagacaga atacttgacc aatttatctt ctggttctgg cataacaatc
     2221 tcattgcttt ttgcaaagtc actgacgtcg gaacctccac gcgcagtcat aggtcctgga
     2281 gctggtctga agctagaccc aagcagcata agcatacatc caacggaaaa catcagcttc
     2341 cctaagtttg atccccgtcc gatatagtat acccagttgc tttgatccaa ctttattcgc
     2401 attctaatcc agaacctctc cctatgatga tcaaatatta taagggcatt caattgaatt
     2461 aggcaagtac gattttttcg aatcgatttg ccttgataga agctcgtgct ttatctatac
     2521 tctggtctca ggaatattgc atgaactcag agagagcgga tgttgcatta atcagatgtg
     2581 atgactatga atctaagaaa ctggatagag cgattgaaac atgtgcggag attgcaggtt
     2641 tccctgatat aaaagataag attatcctca taaagccgaa tgttttaaat gcatcaccag
     2701 cctctaaggc cgttacaacg aatcccgctt ttgtcgaggc agtcgttcga tttgtaaagt
     2761 ctagaggcgc aaaggaaatt ctagtaggag attctccagg atggcaacca gttttgcttg
     2821 ctgcaaagac ttctggaatt tacgacgcgg tccagcagag cggcggcaca tgggttgatt
     2881 ttcgcgaggt ttctcctcat cctgtcgtag atggcaaaaa gctcaagaac atcccactca
     2941 cggctctact cgagaaggtt gatttagtca taaatcttcc aaagctgaag aatcacagtc
     3001 ttacgacata tactggtgca atcaaaaacc tctttggact tatccctggg acagcgaaga
     3061 gcgcaatgca tcttcaatat cctgctgtca tcgattttgg tgagatgctt gtggatctgg
     3121 ctttgtctat tcctaattgt tttactttta tggatgcaat catcgctatg gatgggcaag
     3181 gccctggttc gggaacgccc tactcgcttg gcattgtact tgcatctatg agtgttgcaa
     3241 agctggactg gattgcgtct cagtgtgtgg gttatgatcc taagaatata tcatatattc
     3301 tagattccat gcagagaaca cttggaagcg caaatgttcc agagccttca acatacccat
     3361 taagagtttc agaagtagaa catagcggtt ttaagcggct tccttacaca agcaagcttg
     3421 gtaagagact cagttcaata ccaaggggtc ttcgttcttt tgctgctaag tttatacacc
     3481 ttagaccaat atttcataag aataagtgca taggctgtag tgcatgcatc acgatatgtc
     3541 cagcaaaggt gcttgtgcta gatagaaaaa atggctcaaa catcatcaga atcaatgaca
     3601 aactctgcat cacatgtttt tgctgccatg aggtttgccc agctaaagca ataagtgtag
     3661 gacgtgcacc gatacgcatt gtgcataggc gcttgcctgt caattaatat ctaggctgta
     3721 tccatggcta ccagagccag caaaggaggt aaaagagtgg ctcatttcag tcttgatgat
     3781 ttgattcact ttggaaactt atccgatttc gaaattatgg atctgcccgc tcccatggaa
     3841 aagaatggcc atttggttct tctagccgat gcgattcgcg agtgtgcccg aatcgcattt
     3901 actaaaatcg cttatcttat gccaaaaacc cagtatgatg cttttgtcaa tatcatcgaa
     3961 gatcctgcag ctagcgagcg agagcgattt gtcgcagctt gtcttctaga aaacgcactg
     4021 atttctggaa aaggaagata tccgatatgc caagatactg gcacagcact tgtctatgga
     4081 tggcgcggta accaggtaga aatagaggaa aatattggct tagaagaatt acttgcccag
     4141 ggctcggcag aagcctggtt gcaggaaaaa ttacgtaatt ctcagatggg ctcttttaac
     4201 atgataaaag aatgcaatac aaaggacaat ttgcctgcag gcatccaaat ccgttctata
     4261 aaaggaaaaa tgctcaagct ctcctttgtt gccaagggcg ggggttcagc ttctcgcact
     4321 tcgctcagca ttgaaagccc tgctattcta agagaagaca agctggagaa agtgcttcaa
     4381 tctcgtatca aggcattggg accttctggt tgccctcctt atgtgatcgc aatggtcctt
     4441 ggtggtacaa atccctcgca atctctgtat gccgcggaac tcgcagccat aggtcttctt
     4501 gatgagcttc ctatctctgc tcaaggcgat ggtatgccca taagagatat actctgggaa
     4561 cagaaaatgc tgacaatagc taaagatagt ggatttggag ctcagtgggg aggttctcat
     4621 tttgctgtct ccacaagagc tgtaaggtta tcgcggcatg cagcaaatct acctttggca
     4681 atcgcagtgt cctgtgcagc aaatcggcat gtctctgtat ttatagataa ttccggatgg
     4741 catatcgaaa aactctcgtc tccttcacaa tgcttagaaa agaaggttca agatattatt
     4801 cagcgaacaa cagccaagac tgaaataatt gaaatatcgc aagtggataa tggctggctt
     4861 tcaagattaa ggtcattgca agcgggttcg atggctctta tctcggggcc tgtactcttg
     4921 gcgcgagata tggctcatgc gcgccttctc tgcatgctcg agttagataa aaaactgccc
     4981 gattggttca tcggcttacc tgtattctat gctgggccaa ctgaggctaa gccaggagct
     5041 gcaactgggg cctttggtcc tacttctgct tcgaggatgg acagctatct tgaagccttt
     5101 atgaaagccg gtgcttcgat tgttacaatc gcaaaaggaa gtcgcagcga tgaagcacgt
     5161 agagctatat ccactcatag aggcgtatac tgcgctgcca tcggaggagt tgctgcactc
     5221 aatgcagagc atcacttacg tacgctagat acaattcagt tcgaagatct cggaatggag
     5281 gcagttcgta tcgcggtact agatagactg cccatcattg tagccatcga ctcacatgga
     5341 aacgatatat ataatctata agctcgctta ggccttaata gaggccacaa gtctatctgc
     5401 gaggtacaag gctgttacca ttagagcatg ccttagttcc ccttctccaa tacgacttcg
     5461 cacttcgtcc gcaggcatta gataggcatc gatgatttca ttttgatcga ggtgctggtc
     5521 aactgcaggt tttggatcca gcgccacaaa aacatggaaa tgattgtcct gaattgcagg
     5581 atttgggaat acggtgcctg catggagaat ccgatctgaa acccaaccag tctcttcagc
     5641 aagctctcta gctgctgcat gagaaggttc ttctccatac tccaccacac cacctggaaa
     5701 ttcgaggcta agctcctccg ctccatggcg atactgtcgt accattagaa atgaagtacc
     5761 ggcatctgtt cgcacatatg gaacgattac tgcccaatct tgggctctca tcaaagaaaa
     5821 atggcctatt cgtccatcag ggccttggct ttctctctcc aatacgctga acacattgca
     5881 ttcatagact ttacgagtag atatttccat ccacctacgt ccgccatttc catcgcctgc
     5941 aaccatatca ttctccgcta gcatcctatt ctcgatggca aagatgggca agctctttag
     6001 tgtgttgaaa tgtgatcgca ccgaaaaatg cccagacttt gacataagat tagttatgca
     6061 aaactagata ttattattcg acttttttgg ttttgatttg cttgtcttaa ttcttttaaa
     6121 gccttataat cagcgaaatg aatatggaat ccttttgccg actgcgccat aaggcgctga
     6181 agatcttatt tctgatgggc gcaagccttg cgctcttcgg tcaggacatt cctgagaata
     6241 tatctatgga gaaactcatc atgcaatctc atcgtcctct cgtcattgct catcgcggtt
     6301 ttagaagcat tgctcccgaa aatactcttc tcgcagcaca gaaaggtttt gacgcaggtg
     6361 ccgatatgtg ggagctcgat gttgcagcct cgagcgatgg cgaattggta gtcttgcacg
     6421 acaacacgct tacgcgcacg acagatgcta aaactcgctt tccatctcgt tctccatgga
     6481 ccgtttacga ttttacgctt gcagaaatca aatctctcga tgctggctca tggtatgccc
     6541 agacagatcc ttttaagcaa attgccattg gaagagtaaa aacttctgag attgcatctt
     6601 tcaaggatct taaaattcca actctacgcg aagcactcga gttgacaaag cgtttgggat
     6661 ggcgagtcaa catcgaaatc aaagatgcaa ctggatttgc ttgcgatgct tggatcgtcg
     6721 agagaaccgc tgagctgata aaagagctcg aaatggttcc ttcggttttg atttcatctt
     6781 ttaatcatga ttacttaaaa cgaatgaaga aggctatccc agagatagca gttgctgctc
     6841 tagtcgacag accgctctct aatccagtgg aaagattgaa agatatcgga gcagtagctt
     6901 taaatccgaa cgaaaaatat ctcgatgaag caacagttaa agcactaaga tccgctggtt
     6961 ttggtgtact tccatggaca gtaaacgatg cttcgaggat gaataccctt atcgattggg
     7021 gtgcgaccgg tcttataacg gatttccccg ataaagccct ggaattggta aacaaaacct
     7081 tttagagaat ttttaattta agctttatat tgcgtgctca gaaaaactat aatatactat
     7141 ttctcgtaaa gagtccggag gctgatccgg taatccataa taatcctgga ggcagagtaa
     7201 tgaaaagaat cgttttgctg atagccgttc tactcatggc tgtcggagct tatgcccaga
     7261 caacggtcga attctggcat gctatggctg gtcataatgg cgaaatggtg caaataatca
     7321 gcgatcaatt caatgcaagt caaaaggaat tcaaagtagt ccctgtttat aagggatcat
     7381 acgtagacac aatgaatgct ggtattgccg ccttccgtgc agggcaagcg cctgcaatca
     7441 tacaggtcta tgaagtcgga accgccacca tgatggcggc caagaatgcc gtcaaacccg
     7501 tctaccagct tatggcagaa aataacatga agtggagctc cgatatctat ataccagcta
     7561 tcaaatccta ttattccact tctgatggcc gaatgatttc gatgccattc aacagttcca
     7621 ctgctgttat gtattacaat aaggatgctt tccgcaaagc tggactcgat cctgaaaagc
     7681 cgcctgtcac ctggcctgaa ttcttcgagg ttgcgaagaa actaaaggct tccggcatgg
     7741 aaggtggctt tacaaccaac tggataagct ggattcagct cgaaaacttc tgctcatggc
     7801 acaatattcc attcggtact cgtagcaacg gatttgatgg cctcgacact cagctcgtat
     7861 tcaatagtcc agtagtcgtt cgccatttcc agaatatcta tgacctctcc aagactggcg
     7921 tattcatcta tggtggccgc gaaaacaaag ccaatcccct cttcacctct ggtcgtgtgg
     7981 gacttcattt tgagtcaatc ggtggatatg gaaacatgaa ggcaacctgc aagttcgatt
     8041 ttggtgttgc gcgcttgccc tattatcccg atgtgcccgg tgctccacaa aattcctgca
     8101 ttggcggtgc ttctctctgg gtcttcaatg gcagaccagc tgcagaatat aagggagtcg
     8161 ccgaattctt caatttcatc tccaagcccg aaatccaagc tctctggcac aaagaaacag
     8221 gctacctgcc tgtaacccaa gccgcctatg agcttacaaa atctcaaggt tactatgtca
     8281 ccaaccctgg actcgaagtg gcgatcaaac agctgaccaa caaaacgcct actccgaact
     8341 caatgggtat tcgctttggt aatatgccca taattcgcga aatcgaagac caggtatggg
     8401 aagaaatcct ttctggaaag atctcagtaa aagatggcct cgacaagatg gtacgcgaag
     8461 gaaacaaaca attgcgcgag tttgagaggc tcaataagta gatgaccagt tccgctctgt
     8521 gaattttcaa actctcagag cgggcaagaa aaagctatta ttgttgcaaa aaggccgccg
     8581 gtcggcggcc ttttctaagc gcaggaattc aaaatgaacc ctcgctatac aacatttttg
     8641 cggtttagta taataatcaa aatcttcttc aggaactgct atgtccaaac agtatgagtt
     8701 caaagcttct atattgcctt atcttctcgt gctgccacag atggtaatcg tttttctctt
     8761 tttcttctgg ccttctgctc aggccatttt gcaatccttc tatctacaag atcccttcgg
     8821 cggcaaaatg atttttgttg gcttcgaaaa ttattcgaaa ctactagttg atcccgatta
     8881 ttggcaatct ttctgggtat cgacggcatt tgcgctattc ataaccttaa gctcgatggt
     8941 tatttctctt tttctcgcaa tccaggcaaa taagaaaata agattcgctt ctttctataa
     9001 gacaatgctt atctggcctt atgccgtatc gacactcgtc gctggagtaa tatggctttt
     9061 catgttcaac cccaatgtag gagttattgc atatgtgctg aagcatactt ttaatgtcaa
     9121 ctggcaatat ctattgaact tcaaccaagc tttccttttg ctcactatgg ctgctacctg
     9181 gaagcagttg gcctacaatt ttgtattctt tcttgcggga ctacaaagcg tccctgcaac
     9241 tctagtcgaa gctgcagaga tagacggtgc tgggcctacc aagagattct ggaaaatcat
     9301 attccccctt ctttcgccga ctactttcta tctattggtc atggaccttg tatatggatt
     9361 cttcgagacc ttccctatcg tgcattccat aacatcggga gctccgggga aatctactgc
     9421 catactcgta tacaaggttt ggcgagatgg ggttatcaat cttgacctag ggagctcttc
     9481 tgcacaatcg gttatcctaa tgataatggt cattattctg acggtattcc agttccgctt
     9541 catagagcgg cgagtcacat actgaagcgg aggaaacatc catgaaagaa caacaacgaa
     9601 ttaataaagc cgctcaccgg gcaaagatca ttcgcagttt ttacccgcat ataatcctat
     9661 ggattggcat agctattatc atattcccga tatatttcat tttttcggct tcatctcata
     9721 ccaactccga tatacaagca gcgcccatgc ctctaggcat aggatctcat ctaatcgaga
     9781 attaccgcca ggcactgctg catggaacac agaacatggg tacgccagtt tctactatgc
     9841 ttaagaattc gctcatcatg gcgcttggca tatctatagg caagataata atttctctta
     9901 tggcggcctt tgcaattgtg tttttcgatt tcccattgag aggcttttgc ttctggagca
     9961 tctttatcac tctaatgttg ccagtcgaag tacgtattat gcctacctac aaggtagttg
    10021 cagatctagg attgctgaat agttatgcag gtttaattct gcccatgatc gtttcagcga
    10081 cagccgtatt cttatttagg caattcttca tgtcagtacc aagagaaata gccgaagcat
    10141 cgcagataga tggcgcaacc ccaatgcaat tctttagaca tattcttgcc ccgatgaccc
    10201 gcacgccaat cgcttcaatg ttcgtgatcc agtttattta tggatggaat caatacttat
    10261 ggccactgct cataaccact aagccagaat tttatacact gctaataggt atcaaccgga
    10321 tgatgtctgg cgcagatgtc cagatagagt ggcagatagt aatggcgaca acaatgcttg
    10381 ccatgcttcc accagtcata gttgtagtgc ttatgcagaa acagttcata tccggtatga
    10441 ccgaaacaga gaagtaatct tagaggattg aactaacaga tcgcggttaa gaatacaaaa
    10501 taaaggctta aaaatgaaaa ggggttgccc aagatttacc tgagccgccc cttcgatttt
    10561 cattcatcag attttttgtg ttaaaatcaa agcatcccaa aataacaacg tgcattttcc
    10621 caacagatgc cgcgcacaag ctgttctgta taaagcggat tatctggcag ttctccagcc
    10681 tccacccatt ctcccactat attgcataaa agacggcgaa agtattcgtg cctcgggaaa
    10741 gacataaaag atcgagaatc tgtaagcatt cctacccatg cgcccaataa cccgactcca
    10801 gcatactccc tcatgtggcg ttccattcca tccttctggt cattgaacca ccaagctgca
    10861 ccaagttgaa cctttccagc tacctgtggc gagctaatct ctggagcggt gcctgcaaag
    10921 ctccctgcaa gtgtagcaag aggattatgc ttggaagggt cgagtgtata gagaatggtt
    10981 tttggaagat tcccctctct ttccaaggct ccaagcaaag aggcaagcgc accaataatt
    11041 ggtgcatcgg aactgcaatc tccgcccaca tcggggccaa aggctctata taatccggga
    11101 tttacatttc tgagcgctcc tatatggagc tgcattgtcc atcctttttg tgcattgagt
    11161 cgtccaaagt gcaaaagaag tgcagtatcg agcttttccc gctctattgg cgataagtct
    11221 tcgccactgc gaccttttct tacaatagaa tcgagctcgg catcggaggc ttgcataaag
    11281 ggtggtataa gaagagcatg atccgacagc ctgctcccca tcgagtggaa ataagcatgg
    11341 cgactggcca aagcatctgc aaggctggca aaggactcaa tttctatgcc agctgcctcg
    11401 ccaagcaaat cgatataagt attccaagcg cttatatcct cggttttcat ggccttgtcg
    11461 ggtcgaaagg caggcaatac ttttgttgtg aatgaagagc caacccgttg ctcctcagca
    11521 attgctctgt gccaagctag attgtctact ggatcgtccg ttgtgcacaa tacgctaacc
    11581 ttaaatcgct ctagaagaga acgggggctc attcttttct ctgctaggat aaaattggcc
    11641 ttctctctta tgctcaatgc attctgaggt gtaagcactt catctattcc aaatattctt
    11701 ttgagctcga gatgagacca ttgtacaaga ggattgccag ccgccttagt taaggcatta
    11761 gcataggcga gaaatttttc gtcccaagct ccagcgcctg cacaaagcga ttcaggcgcg
    11821 ccattcgccc tcattatgcg ccatttatag tggtcccctt ccagccaaat cttggctata
    11881 tcatcgaaag gtttatcttc ggctatggcc tttggtgaca agtggcaatg ataatcgaag
    11941 ataggcaaat ctttcgcaat ttcgaaaaat agccgttttg cagtttcatt cttcaacaga
    12001 aaatcctcat cgagaaattc agccatataa ccccctttct atattgcaga ttggaatata
    12061 gttgactgag atattgtaga ttaaaatatc gcgaatttag atattatcga tgaggaatcc
    12121 tccatcgatg ggaatgctaa ctccagttac aaagccagat gccttcgaac tcgctaggta
    12181 gagcgcggtg ccccaaagct cttcatattc tccaaaacga ccaaacggtg tacgcgcaat
    12241 aatcgctcgc cctcttggcg tgagatcgcc tgttttttca tctatgagaa gcgcacggtt
    12301 ctgattaccg acaaaaaaac cgggtgcaat agcattgacc ctaattccat atggggcaaa
    12361 ctcctttgca agtgcttggg tgagattgag cacgccggcc tttgcagcgt cgtatgccca
    12421 agtatccgat aggggcttgt agcttgtcat cgaagtcatg ttgatcatag agccctttat
    12481 tccctcagag atccatcgct ttgcaaagac ctttgatggt attacaagtc ctgccataag
    12541 gtttagcctt aacacttcct cgaacaattt ttcatctaat tctacaaatt tcgatctccc
    12601 aatattgcca ccaacaccgt tgagaagaat atcgggcacg ccaaggctct gaattgtctg
    12661 ttccaaggcc tgttcgcagg cttctgtaga gccagtatcg actgtgatac cgataatatc
    12721 agcgctatct aaaggcaaat cagcctgctt tctgactgtt ttagcggcct cttccatggg
    12781 atgatgagtt ccactgcccc agagagctac ttttgcattg gccttggcaa aggcaattgc
    12841 aatataagat gggagcatgc ctcctccacc tgttaccacg gcgatcttgc cttccagact
    12901 gaacattgat tcaacaaacg acatcgaaaa actccttata tttgaatata tcctaaagta
    12961 ttagctaagt cctccatttc tactgcccag gcattattag gttcggaagg aacaatgtta
    13021 tctcaggcac ataagtcaca aggaacaatg tcgcaatcat ggcaatatag aatggaatca
    13081 tcgccttcga cgccttttca atagatattt tgccaatcgc acatcccaca aaaagagcag
    13141 accccacagg cggcgtgcac aatcctatgg caagattgat catcaacatt atgccaaaat
    13201 ggatgggcga cataccgaac ttcccaatca atacaggata cagtattggc gtgacaatga
    13261 gaatgagcgg cgccatatcc ataatcattc caaggaagag aagaagcaca ttgacaagga
    13321 agagcagcat gtacttgttg tccgtaatcg aaagcaagaa ttctgttgcc atagcgggaa
    13381 ttcgcagata ggccatgagc cacgagaaag agcttgctgt ggcaatgagt gtcatgacca
    13441 ttgtgagcgt cttgagagta ctatagagaa tgcctctgaa ttctctgagt ggaatctgct
    13501 tatatacaaa gaatgtaatt ataaatgcat agactgcggc tactgcagca gattcggttg
    13561 cggtaaaaac acctgcaaca acgcctacaa tgattatcac cgcgacaaac aatccccaaa
    13621 aagcatcgat ggctatcttg attccttctt tgaaagaata ttttcttccc ttaggataat
    13681 ttcttttaac tgaaagaata tacacaataa tagctagaga aataccaagc agaataccag
    13741 ggatatagcc accaaggaaa agcctcccaa cagacacacc gccagctgca accgaataga
    13801 taatcatatt gtggctggga gggatgatta tcccctgcgt tgcgctcgtc acagttatgg
    13861 caaccgaaaa atcgttgtcg tagccgtttt tattcataag aggaatgata atgacaccgg
    13921 ttgaggcgac atcggctacc gcggagcccg agatgcctcc aaacaacata ctatcgacta
    13981 cgttgaccat gccaagacct ccgcgaaccc ttccgacaag gacattcgcg aggttaatga
    14041 ttttttctgc tatacccccc cgtgccatca tctcgccagc gatgatgaag aagggaatgg
    14101 caagcaaaga aaatgacttt acaccctgta ccatctgctg cacgatggcc attaatggta
    14161 tatgtagata gaaagcggtc gctatggaag ccgtagcgag cgaaaaagta atgggtactc
    14221 tcaaaagaag caaaagaaca aatccgccca aaagaattat cgtgggaatt gtcacattca
    14281 tagcgctagc tcctcaatat atctctgatt cttccacctt gaccggaaaa gtatgcttcg
    14341 agtttttcgt cgaacatatt gaatccgaaa atatgaaaaa tagcttcgac aattgccaca
    14401 aagccggaca acggaagaac aaggtagaga attcccgctg gcagcatagt tgcaggcata
    14461 attgacctca tagtgaactt cacaagcttg cttccatata taatcatgat cgcgccaata
    14521 aagatgtaga taatatcggt aagcatatcc aaagcgcgat ttagagaggc ggaaattttt
    14581 tctcggttaa caatatttat ggaaaaatgc aggcgatact ttactccaag ccccaaagaa
    14641 agaaaaatga accagacctc gagcacaagt gcgacttctt ctgaccagta gattccgcta
    14701 ttgaatacat agcgaagcac cacattgata aagacaagaa gcagcatcgc gacaatcatg
    14761 atcatggaga gatttattaa tagaatatgg ataaatgtaa ctgcggagac aatgaatttt
    14821 ttcatgataa acctcagttt taggcataga aaactatatc gaaatgcgcg caaggctgca
    14881 ttgcatactc ggcagactca gatacgcaac cctgcgtgca tctcgacggc ctcgccaata
    14941 gaattcaata tttatttcac agcgcgaatt cttgcgacga gatcctgaat ctcttttggt
    15001 tgctgcttgt acataggctc cactgctttc atccattccg tcttgtcggg tattggtatg
    15061 atggtgcagc ctgcctcctt gaccttatcc atggaaacct tgacataagc atcccattga
    15121 gcgcgctgat agtcgatggc atccattgct gccttcttga ttaaatcctg atcagctttc
    15181 gacaatctgc caagagaaat cttcgaaccg atgattatct ctgggaccat cgtatgctcg
    15241 tctaaagtat agtattttgc aacctcataa tgacttgcag aataatagga tggtgggttg
    15301 ttttctgcgc catcgactac tcctgtctgc agaccagaat agacctcgcc gtagggcatt
    15361 ggcgtaggta tcgcgccgaa cgaacgcact agccccaaca ttagctcgct ctcttgcacc
    15421 ctgattttca taccctttag atccgcaggt gtgtagactg gctttttgct attgtagaag
    15481 cttctagctc ctgcttcgaa ccaaccaagc ccaataaaac caaaaggttc gaggctcgca
    15541 agaagctcca tgccgatatc tcccttcaac accttccaca tatgatctga gtctctgtaa
    15601 aggtaaggca tctggaatgc attgagtttt ggaacaaagg cggctactgg gcttatagat
    15661 acacgagtga tgtctatggc tccgaactgc acttgctcga tcacagcctt ttcttcgcca
    15721 agttgagagc caggatagac ttcaacaatt attcggccat tcgagcgttc ctttacaagg
    15781 cgagcgaatt cctcatctcc caattccgtg ggatagcctt tgggatgggt ctcggcaagc
    15841 ctcaatacaa tcggcttagc ctgagcaaac aacatcgacg gtaagaggac aagcaacaag
    15901 gcaagcgcga taatacgaaa ctttttcata cgcacaacct cctttagaat ctaatctttt
    15961 gcagatattt gagatatacc tgcaattagt aattatagga ggctattttt cgttttgtgt
    16021 ttggtaattc catgcctatt ctt
//
LOCUS       DAOSOB010000048        15548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3815_length_15548_cov_68.814626, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000048 DAOSOB010000000
VERSION     DAOSOB010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15548
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3815_length_15548_cov_68.814626"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLS88_06165"
     CDS             <1..500
                     /locus_tag="PLS88_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOU06960.1"
                     /translation="NAYIDKEDDNYSDFRDALQFVCVELAKMVARDGEGATRLIECTV
                     GGAVSEEQAESLAKGIITSSLVKTAMFGADANWGRILCAMGYTRSDFNPDKVDISFES
                     PRGYIEVCKGGAPLPFSEERAKKILNNQEVEIIVDLHLGEAEVTAWGCDLSYEYVRIN
                     GDYRT"
     gene            612..1472
                     /gene="argB"
                     /locus_tag="PLS88_06170"
     CDS             612..1472
                     /gene="argB"
                     /locus_tag="PLS88_06170"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOU06961.1"
                     /translation="MDKHFNADVLIEALPYLRKFCGKIIVIKYGGAAMINEGVRQTVI
                     QDLILMQQVGMKPVLVHGGGPEIDRMLKKLNKEAIFVNGLRYTDDETMEIVQMVLAGK
                     VNKDLVELFSRMGGKAMGICGSDGGLFMAKRMRKNGQDLGLVGDIQEVKTDAIRMLLD
                     NGIIPVVASIALKADEGSGFYNINADTAAGALAIALKAEKLILLTDVPGVMEDRADLG
                     TLIREMDVEMAKTLMKKGVIKAGMIPKVEGCLQALAQGVTAAHIVDGRSPHSLLVELF
                     TDTGMGTMIR"
     gene            1514..2683
                     /locus_tag="PLS88_06175"
     CDS             1514..2683
                     /locus_tag="PLS88_06175"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOU06962.1"
                     /translation="MSDRFMNTYARTGLVLDHGAGARLFAVDGNEYIDFMAGIGVSSL
                     GHGHPKLVAAIAEQASKLIHVSNYFITEPSIKLAQELTDATGYDKVFFCNSGAEANEG
                     MFKLARKYGSTKNPNKNIIVTLKQSFHGRTITTITATGQDKFHQYFGPFTPGFIYAEP
                     DDIEVLDKLLGPNVCAFVFEPIQGEGGVRMISQAYLKAAQEMCRERDILFCADEVQTG
                     MGRTGAILACERLGLKPDAVAIAKGLAGGVPIGAFLARGNAAEVLQPGDHGTTFGGGP
                     LAASAALVVLSELKRPGFLDEVDRKGRRLRSLIESFHHPIVKDVRGMGLMIGVGVKVD
                     PHEVVSAAQAHHLLVLTAGEDTVRLLPPLILTDKDIEEGAAALKLALDDVDKLRK"
     gene            2868..4124
                     /locus_tag="PLS88_06180"
     CDS             2868..4124
                     /locus_tag="PLS88_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOU06963.1"
                     /translation="MNTDAWLYFGIVLGFVFSGSSGHALHGLYTALAYGFGASSLALL
                     AKAGGGIYTKTADIAADLVGKVEIGIPEDDPRNPAVIADNVGDNVGDVAGMGADIFDS
                     YVAATVASMTLGASFAQTIGVQYIVLPLIMCIIGIISSLIGLQLVHVGPNGKPGKALN
                     SGSVFSCFVFIVLSVLVFAITNSVAGSMVLNWGALIATISGLLIGIVIGFSTDFFTND
                     EYKPVRHVANVSSSGTGLNIITGFSYGLISMIPAIVGIVVAMMAAYFGCEAFGLHGFY
                     GIEISAVGMLSLTGIVVSSDAYGPIVDNAKGIAEMSGMKEEVVDAADSLDSVGNTAKA
                     INKGFAISAAALTMLAVTRNAFTMINEIRRQFHEKPWILQGTDLPDYSACVGIASQGA
                     LLALALPAFLAVVLPLCAAYCAVPYF"
     gene            4380..4640
                     /locus_tag="PLS88_06185"
     CDS             4380..4640
                     /locus_tag="PLS88_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06964.1"
                     /translation="MEQNYKVFPYRWVILMATMLVNMTIQVLWIAYSPITSSAALYYG
                     VSEMAVGFFAMSFMIAFIPLSIPASWLIDRYGYVLTVGAASA"
     gene            4669..5250
                     /locus_tag="PLS88_06190"
     CDS             4669..5250
                     /locus_tag="PLS88_06190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU06965.1"
                     /translation="MVEGLKHALKTPSFVRFLVIVFVGMGIFNGVTTWVEGIVRPRGI
                     GLDQAGILGAIMLVGGIIGAMVIPHFSDKAAKRKPYIVLGLIGAIPGLVGLALAPGFV
                     LIAASSFVLGFFLIAVNPVGMQYAAEVAHPTPEGTSNGLVTLAGQISVLLVYAMEAIN
                     NVTGTFTVSLIIVAVLLGGSAILATSLKENDQR"
     gene            complement(5478..6641)
                     /locus_tag="PLS88_06195"
     CDS             complement(5478..6641)
                     /locus_tag="PLS88_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU06966.1"
                     /translation="MIGRKMNRLFNEALSIGVAALSKKVLSKKNFSKKASPIEAYSIE
                     AQKRKPNSNLSRHKNALPLRCKTTFIFAMLCIVFGIRTVSITWAQAESPAIADRYDQV
                     FSRENNEKSLTAWFLRLLLSEESNTDEKSGDSTIVISPDNKVMLIDGGAPSCGAMVCS
                     YLHALGIDRIDAIVVSHPHIDHIGGLISVLQEFPVEVIYMSKLEYPTAIFQSFLTNAA
                     EKEIPIIYLEEGSEFDLGSKIHAKVYNPEANIAYYSGYPANSTLFVNDRSLVIKFAFG
                     NSSMLFMGDVYTSRELDLIEKYGSELKADVLKVGHHGSDTSSSKSFIRLVSPEIAVIM
                     HDRLASLQVYNNLRKIGAAVYLSPIDGNIRVSIDDEGNMSVITQFDRISDFLK"
     gene            complement(6638..7507)
                     /locus_tag="PLS88_06200"
     CDS             complement(6638..7507)
                     /locus_tag="PLS88_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU06967.1"
                     /translation="MLKQKEISNIKAIRTRHILTFFQYLFAIVFTLIIVFPFYWMIIT
                     SLKTSEELLRPVPTLWPLKFQWKNYIDVFKMAPFGQYILNTIIMTTGVMTLQIVIGSF
                     AAYGFSKGKFPGRDILFIVVLGALMVPIQVTFVPIYVMIAKLNWINTFAGLIVPNAVS
                     AYTIFMLRQTFKSVDDAFIEAAQLEGLGRIGIIFRILIPLCKPTMLTVTIITFIDSWN
                     AYFWPKIATTTPLRRTIALGVYELKRSYAGLETMNQNQIMAGAVVTVIPIIILFVLLQ
                     KYILTGFSKVASK"
     gene            complement(7523..8428)
                     /locus_tag="PLS88_06205"
     CDS             complement(7523..8428)
                     /locus_tag="PLS88_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU06968.1"
                     /translation="MTTKKKINYRRREKLKDFLCVLPAVIFFALFVYYPILKLFQISF
                     TNWNLISDTYKYVGLKNFKWLFKGSGFASLINSLTITFRYTFWEIVITIAGGLLLAAL
                     FNRESKVFSALRSIVFMPRYIAISASAIVFMWILNDQYGILNYVLGVIGLKPIMFNTK
                     ENALTAVLILTGWRVIGYGMMIYLSAMKGISPEYYEAAKLDGANGFQRFRYITLPLLS
                     PTTLFLFVTTFIASMKVFQSIDVMTSGGPYGATEVIVYWIYELAFVDFRVDRAAAVTL
                     VFFVILMGFTAATMKFTERNIYYES"
     gene            complement(8524..9801)
                     /locus_tag="PLS88_06210"
     CDS             complement(8524..9801)
                     /locus_tag="PLS88_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU06969.1"
                     /translation="MKKGISTFFLSMMMLMVIVGIGAQDADKYAVKAPIKIQFWHALE
                     AQYKPLIDKVIADFEKQNPLIKVEAIYQGSYADLNEKLIAAQAAGTTLPALTVANTPY
                     VAQYGAAGLCEVLDPYIKATGFDINDFGEGLRKASSYDGKQVSLPFLISTQIMFYNKD
                     MAEAEKIKLPEKWSDMDEFMKKASKISGGVTQRYATVIPGWDQWYFEPFYLNAGVKII
                     KDNNTTDLGEPTAVAITKKLKQWCDEKSAYWAYGKDASSIMRQNFLDGKAFSVVHTTS
                     LYEMYATNAKFKVGMHYLPGNVTRKSEIGGCVLLIPSKNKQDVKNAAWKLLSYLTSKE
                     VNMLWAKETGYMPTRNSVLQTSEAKAFLEEKPAFKAVFDNLNNINPRIQHPAYAALSK
                     IWMQELAKVIIEGGDVDSSMKKMAQLINEALED"
     gene            10092..11216
                     /locus_tag="PLS88_06215"
     CDS             10092..11216
                     /locus_tag="PLS88_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOU06970.1"
                     /translation="MNNEKYSRRRSALAAELMRRGLSAVMISDFEHGRDSSLRYLCGQ
                     PSDALLVVNSNGTSVLIAWDVNMAHRLGDADHILPFTDFGRQSAQALKAAFELLGIEK
                     GSKVALPASTPYPEYIELVQSLDAFDLVCEKNGIDGFIKEMRSRKDADEIDLYWMIAK
                     YTDELMDMVEKEVAAGTLSTELDVALFLEREARRMGAEGMGFESLVAGPERSFGIHAF
                     PTFTAGPFANKGLSILDFGLRFEGYTSDVTMSFVDNLLNQEQKTMIDLVEEAHRIAVE
                     ACGPGVSLLKVATLVDEFFANAGWFMPHSLGHGIGLDAHELPTLSMRAMPDDLLLPGH
                     IVTIEPGLYHPELGGVRLENDVLITDTGAEVITHSRIVRL"
     gene            complement(11226..12311)
                     /gene="pyrC"
                     /locus_tag="PLS88_06220"
     CDS             complement(11226..12311)
                     /gene="pyrC"
                     /locus_tag="PLS88_06220"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOU06971.1"
                     /translation="MAKEITKDKADIDRFDMPLPDDFHAHLRQGALLKASVLGHAGQF
                     ARVLLMPNTLPPVASPSRLKAYRSEVDQVLAELPLEKRFEPLYTFKLLPGMKGYDIEA
                     LVQKGAIAGKYYPSGSTTNAADGPRSFDEVEDALNVMEEMGLVLCIHGEDPSAPILER
                     ERAFLPQVERLILRHPSLHVVLEHLSDARSVRFVQQGPQNLAATITAHHLLFTLDDMM
                     ADSLNPHLYCKPVLKFARDRDALRMAVLSGSNKFFFGSDSAPHPRGKKECSAAASGVY
                     SAPTALPALVELFADLGGLDQLVPFLSENGARFYGLAAKPSVKGNEQQDEDSRSIAFQ
                     RMPWTVPETIDGLVPMCASQRLSWRVV"
     gene            12375..13649
                     /gene="murA"
                     /locus_tag="PLS88_06225"
     CDS             12375..13649
                     /gene="murA"
                     /locus_tag="PLS88_06225"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOU06972.1"
                     /translation="MDKYIIEGRYPIKGTLTLSGNKNAALPCIAASVLAQEPVTLRNI
                     PAIEDVAVMFDVFRYLGGLVEQIDTHSWCLDPRSISKCEVPEELAKKVRASILFAGPL
                     VARFGKAILPPPGGDTIGRRRIDTHIVALQELGARISLENTLGFSATKLVGVPVFLDE
                     ASVTGTENAIMAASLAEGTTILMNAACEPHVQDLCRMLIQMGADIDGVGSNRLIIQGA
                     KSLNGTDFTISADYMELGSFIGLAAVTGGDLRIDGVRADDLPPLKVGFSKLGVTWDLK
                     GNTLRPNNMVALRVVPDIGGQIPKIDDAPWPGFPADLTSIMTVVATQADGVALIHEKM
                     FESRMFFVDKLISMGARIVLCDPHRAIVYGPSKLVGNVMVSPDVRAGMAMVIAALCAE
                     GTSIIHNVYQIERGYENLVERLSAVGAHIKRE"
     gene            complement(13739..14446)
                     /locus_tag="PLS88_06230"
     CDS             complement(13739..14446)
                     /locus_tag="PLS88_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOU06973.1"
                     /translation="MGMFDRLKTVISSNINAIISKAEDPEKMLNQMIIDMNEQLIESK
                     KAVAMAIADEKRLEREMMENKAKAQEWEKKAMLAVRANRDDLAKEALLRKQEFENYAT
                     QLAQQWEAQKQSVEKLKEALRQLQSKIEEANRKKNILIARAKRAEAQQRITQTMSSMA
                     GNKSAFETFERMERKIEEVEAQAEATKELEDTTTGASLEKQFAQLESSPQAADVMLEE
                     LKKKMLTEDAGRANSQQ"
     gene            complement(14450..14845)
                     /locus_tag="PLS88_06235"
     CDS             complement(14450..14845)
                     /locus_tag="PLS88_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOU06974.1"
                     /translation="MSGLTRIESYLIDLGIEYQELSNSSWLIEDESKGYPRMIVSLSD
                     PLVIISANVMPIPKENAEALFRTLLELNATDLLHGAYAISGDQIIVIDTLEYNTLDKE
                     EFLASIESISFALLEHYKKLANYIHKVEE"
     gene            complement(14896..15438)
                     /locus_tag="PLS88_06240"
     CDS             complement(14896..15438)
                     /locus_tag="PLS88_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOU06975.1"
                     /translation="MPSGLLSRIQKISQPGSDALISSQISTDFPGCIVARFVGAVTEA
                     NLDTVAQSFDSILSEGIRYLVVDFSTIEDIGPAGMGLMLALRQKLRDRHGDLVLCGMR
                     PCMERMQRILGLEGYFTTAVDAQSALTGLKFAINGIYPLSVQCPACNSLIDIEKPGRG
                     RCQTCEAVITAFPDGTITLG"
BASE COUNT         4014 a         3407 c         3791 g         4336 t
ORIGIN      
        1 acaatgcgta tatcgacaag gaagatgaca actacagcga ttttcgcgat gctctacaat
       61 tcgtctgtgt ggaactagcc aagatggtag ctcgcgacgg tgaaggcgca actaggctga
      121 tagagtgcac ggttggagga gccgtatccg aagagcaggc cgaatcccta gcgaagggca
      181 taatcacatc gagcctcgta aagaccgcaa tgttcggagc agatgcgaac tggggtagaa
      241 ttctatgcgc aatgggttac acacgcagcg atttcaaccc agacaaggtg gatatctctt
      301 tcgagagccc aagaggctat atcgaggtct gcaagggagg cgcgcccttg cctttcagcg
      361 aagaaagagc caagaagatc ctcaataatc aggaagtaga aattatcgtg gatctgcatc
      421 tgggagaggc tgaagtaacg gcctggggct gcgatctttc ttatgaatat gtgcgaatca
      481 atggcgatta tcgtacataa tcgaagcaag gctataatgc cttcgtgtcg atcttagata
      541 aatagaagtg aggcttctta atatgttcgc gtcgacttta gataaagata tgattacgga
      601 cgaggtgctt tatggataaa catttcaatg cggatgtctt gatagaggct ctgccatatc
      661 tgcgaaaatt ctgtggcaag ataatcgtaa tcaaatacgg cggtgcagcc atgataaacg
      721 aaggcgtgcg tcagaccgtg attcaggacc ttatcctcat gcagcaggta gggatgaaac
      781 cagtccttgt gcacggaggc ggaccagaga tagatcgaat gctgaaaaaa ctcaacaaag
      841 aggcaatatt cgtaaatggc ctgcgctata ccgatgacga gaccatggaa atagtgcaga
      901 tggtccttgc tggcaaggtg aacaaagacc ttgtagaact tttttcgcgc atgggcggca
      961 aggcgatggg aatttgtggc tcggatggcg gactattcat ggcaaagcgc atgcgaaaaa
     1021 atggacagga tctcgggctt gtaggcgaca ttcaagaagt caaaaccgat gctattcgaa
     1081 tgctactgga taacggcatc ataccggttg tagcgagcat tgctctcaaa gcagatgaag
     1141 ggagcggatt ctataatatc aatgcggata ccgcggctgg agctctagct atagcactca
     1201 aagccgaaaa actaatcctg ctcaccgatg ttccgggcgt tatggaagat agggcagacc
     1261 tcggtacact catccgcgag atggatgtgg agatggccaa aaccctaatg aaaaagggtg
     1321 taatcaaggc tggaatgatt cccaaggtag agggttgcct ccaagctctt gcccagggtg
     1381 taacagcagc gcatatagtc gatgggcgct ctcctcattc tcttttggtg gaattattca
     1441 ctgatactgg catggggaca atgataagat gaaagatata tcgaataaca aagcgcatat
     1501 gaaggaagca atgatgagcg acagattcat gaatacctac gcgcgtaccg gccttgtgtt
     1561 ggatcatggg gctggtgctc ggctttttgc tgtcgatgga aacgaatata tcgattttat
     1621 ggcgggaata ggagtcagtt cgctggggca cgggcatcca aagctcgtgg cggcgatagc
     1681 ggaacaagca tcaaagctaa tccatgtgtc gaattatttt attactgagc catcgataaa
     1741 actcgctcag gagctcacgg acgcgaccgg ctatgacaag gttttcttct gcaattcagg
     1801 agccgaagcg aatgagggta tgttcaagct tgcccgcaaa tatggctcaa caaagaatcc
     1861 gaacaagaat atcattgtaa ctctaaaaca atctttccat gggcggacga taaccactat
     1921 tacggctaca ggacaggata aatttcatca atacttcgga ccttttacgc caggctttat
     1981 ttatgccgag ccagatgaca tagaggtgct cgataaactg cttggaccga atgtgtgcgc
     2041 ttttgtgttc gagccaattc aaggcgaagg cggggtgcga atgatttcgc aggcgtatct
     2101 aaaggcagcc caggagatgt gccgcgagcg ggatattttg ttctgtgcgg atgaggtgca
     2161 gacggggatg ggcagaacgg gggcgatcct ggcctgcgag cggctgggac taaagcccga
     2221 tgccgttgcg attgccaaag ggcttgctgg cggcgtgccg attggcgctt tccttgcgcg
     2281 agggaatgcg gctgaagttt tgcagcctgg cgatcatggc acaaccttcg gcggcggccc
     2341 acttgcggct tccgcagctc ttgttgtgct atcggagctt aaaagaccgg gttttttgga
     2401 cgaagtagac cgcaagggcc gccgcctcag atcgctcata gaaagcttcc atcatccaat
     2461 agtaaaagat gtgcgcggca tggggctcat gataggcgtg ggagtaaaag tcgatcctca
     2521 tgaggtcgtc agcgcagccc aggctcacca ccttctcgtt ctaaccgctg gtgaagatac
     2581 cgtgcgcctc ttgccacctc tgatactaac agacaaagat atcgaagagg gcgccgcggc
     2641 gctaaaactt gcattagatg atgtagataa gctgcgcaaa tagcaaaaac agcgccgggg
     2701 tggctcgaaa tagagctaac ctaggtttat cgcagcaata acttgtcaac gacttgtcgg
     2761 aacatgaagg gtgaccccta tatcttttag agaattctca gaaaaatatc gcaaagctct
     2821 gaaagcccac cttctctgct tgattagcaa tagataggaa gactcatatg aataccgatg
     2881 catggctcta ttttggcatt gtacttggat ttgttttttc cggcagttca ggtcatgcgc
     2941 tccatggatt gtatacagca cttgcctatg gttttggggc ttcatcgcta gcgcttcttg
     3001 ccaaggcagg gggcggaatt tataccaaga cagcagatat cgctgcagat cttgtgggga
     3061 aggttgaaat aggtattccg gaggatgatc cacgcaatcc tgcggttata gcggacaatg
     3121 ttggagacaa tgtgggcgat gtggctggaa tgggcgccga tattttcgat tcctatgtag
     3181 cagcaactgt tgcatcgatg acgcttgggg cttcttttgc acagacaata ggagtccaat
     3241 atatcgtatt acctctcata atgtgcatta taggcatcat aagttcgcta atcggtcttc
     3301 agctagtcca tgtaggtcca aatggcaagc ctggcaaggc tctgaattcg ggttcggtgt
     3361 tttcatgctt tgtttttatt gttctttctg tgcttgtttt tgctataacg aatagcgttg
     3421 ctggaagcat ggttctaaac tggggagctc tcatcgcaac aatttcaggg cttctcatcg
     3481 gtatcgtcat tggattttcc acagattttt tcaccaacga tgaatacaag ccggtgcggc
     3541 atgtagctaa tgtctcttca tccgggaccg ggctcaatat cattacaggc tttagctatg
     3601 gtcttatttc gatgatacca gctattgtag gcattgtagt ggccatgatg gccgcttatt
     3661 tcggttgcga ggcattcggg ctacatggct tttatggtat agagatttcg gctgttggca
     3721 tgctttcgct tactggaatt gtggtttcca gcgatgctta tggtccaatt gtcgataatg
     3781 ctaaaggcat cgcagagatg tcaggaatga aagaagaggt agtcgatgct gctgattccc
     3841 tcgactctgt tggaaacact gcaaaagcaa tcaataaagg ttttgcgatc agcgccgcag
     3901 cactcactat gcttgctgtg actagaaacg cattcacaat gatcaatgag attaggcggc
     3961 aattccacga aaagccatgg atattgcagg gtaccgattt gcccgattac tcagcatgtg
     4021 tcggcatagc atctcaaggt gcgcttctag ctcttgcttt gccggctttc ctcgccgtcg
     4081 tacttcctct ttgcgccgct tattgcgcag taccatattt ttagttgacc gtaaaatgag
     4141 gctatcctgc tgaagtttga gtgaggctac tttgaatgaa aactcgtcat ctgctagagg
     4201 gctactctct aattaatgcc atattctcta attaggcgaa tccgctttct tgaatgaatc
     4261 tgcctatcat aattcatccc tcatccgtga acaggattct tatcgccttg tcagtatctt
     4321 acagagctgt tggctttgct tcataatata agtattcgag tacaagggag agatcgatca
     4381 tggagcagaa ttacaaagtt tttccctatc gatgggttat ccttatggcg acaatgctcg
     4441 tcaatatgac tatacaggta ctgtggattg cgtattcgcc catcacgagt agcgcggcct
     4501 tatactatgg ggtatcggaa atggcagttg gcttttttgc gatgtccttc atgattgcct
     4561 ttatccctct ctcgattcca gcttcgtggc tgatcgaccg atatggttat gtgttaactg
     4621 taggtgcggc ttccgcctga cgaatcggca tccagcgagc gtgccttcat ggttgaagga
     4681 ttaaaacatg cgctcaaaac accatcattt gtgcgattcc tcgtaatcgt gttcgttggg
     4741 atgggtattt tcaatggtgt tacaacctgg gttgaaggta ttgttcgtcc gcgaggcata
     4801 ggtttggacc aagcgggaat tctcggagcc attatgttag taggaggaat tattggcgca
     4861 atggtcatac cgcacttttc tgacaaggct gcaaaacgca aaccctacat tgtattaggc
     4921 cttataggag cgattccagg tcttgttgga ctggcactcg caccaggatt tgtactgatt
     4981 gcagcttcgt cttttgtgct gggtttcttt ctaattgcag tgaatcctgt gggaatgcaa
     5041 tatgcggctg aagttgcgca tccaactcca gaaggcacat cgaacggctt ggttacgctc
     5101 gcaggccaaa tctccgttct actagtctat gcgatggaag ctataaacaa tgtaactgga
     5161 actttcactg tatcgctcat catcgtagcg gtgctattgg gtggttcagc tattctcgcc
     5221 acaagtctca aagagaatga tcaaagataa tgatgagacg gctcgttagg attgtactag
     5281 taagtatcgt gcatgaagca tttggcatgg agcgcataat aaatgctata tgcttgaatt
     5341 gatataagtt tttcaattct tggtgaactc gccttgcagc caattgtctc tatgcagagt
     5401 atgcaatgcc attttaagtc tataataaca tcttggataa aaatatgttg caagattaga
     5461 ggggcgatat agaattctta tttcaaaaag tcgcttatcc ggtcgaattg cgtgattacg
     5521 ctcatatttc cctcatcgtc gatagagact cttatgtttc catcaatggg gctcaaatag
     5581 actgctgctc ctatttttcg aaggttgtta tatacctgca agctggcaag gcggtcatgc
     5641 attatcactg caatttctgg agacacaagc cttataaaag acttggatga tgatgtatcg
     5701 ctgccgtgat gtcctacttt caaaacatcg gccttgagct cagagccata tttctctata
     5761 agatccagtt ccctcgatgt atagacatcg cccatgaaca acatgctcga attgccaaaa
     5821 gcgaatttta ttaccaagga tctatcgttt acaaatagag tgctattggc aggatatccg
     5881 ctatagtacg cgatattggc ttccggattg tatacttttg catgtatttt gctgccaagg
     5941 tcgaactccg atccttcttc gagatagatg atggggattt ctttttccgc tgcattggtc
     6001 aaaaaactct ggaaaatagc ggtaggatat tcgagtttgc tcatatagat gacttcgaca
     6061 gggaattctt gtagtacgga tataagccca cctatatgat cgatatgagg atgcgaaact
     6121 actatggcat cgattctgtc gattccaagg gcgtgcagat aagagcatac catggcgccg
     6181 caggatggag ctccgccatc tataagcatc accttattgt ctggcgagat aacgatagta
     6241 ctatccccgg atttttcatc ggtattggac tcttctgata ataagagcct taggaaccag
     6301 gctgtcagac ttttttcatt gttctcacga ctgaatactt gatcatagcg atccgctatg
     6361 gccggagatt cggcttgagc ccatgtaatg ctgacggttc gaatcccgaa gacaatgcac
     6421 agcattgcaa aaatgaatgt agtcttacat cgcagaggaa gggcattctt gtgtcttgag
     6481 aggttcgaat tgggttttct tttttgggct tctatagaat aggcttctat aggggaggcc
     6541 tttttagaaa agttcttttt agaaaggacc ttcttagaaa gggccgctac ccctatagaa
     6601 agagcttcat taaaaagccg attcatcttt ctcccaatca ttttgaagcc accttcgaga
     6661 atccggtaag aatatatttc tgaaggagaa cgaatagaat aatgattggg ataaccgtta
     6721 ctacagctcc ggccatgatt tggttctgat tcatggtttc taggcctgca taagagcgct
     6781 tgagttcata gacgcccaag gcgatggttc ttcgcaatgg ggtggtggtg gcgattttag
     6841 gccagaaata cgcattccag ctatcgataa aagttattat ggtaacagta agcatggtgg
     6901 gtttacataa tggaatgaga atgcgaaaga taataccgat tcgtcccaat ccttcgagct
     6961 gtgcagcttc tatgaacgca tcgtctacag atttgaaagt ctgtcgaagc atgaaaatcg
     7021 tataagcgga tacggcgttg ggaactatca aacccgcaaa agtatttatc cagttgagct
     7081 ttgctatcat tacatatatt ggcacaaagg ttacttgaat tggtaccatt agtgctccca
     7141 agactacaat gaatagaata tcgcgtccgg ggaattttcc tttggaaaaa ccataggccg
     7201 caaaggatcc tatgacgatc tgtagtgtca ttactcctgt ggtcatgatg attgtgttaa
     7261 gaatatattg cccgaaagga gccattttga acacatcgat ataattcttc cactgaaatt
     7321 tcagcggcca aagagtaggg acgggtctca gtagctcttc gcttgtcttg agcgatgtaa
     7381 tgatcatcca gtagaaggga aagacaatga taagcgtaaa tacgatcgca aacagatact
     7441 gaaaaaatgt cagtatgtgc cgagtcctga tcgctttgat attggaaatc tctttctgtt
     7501 ttaacataag taagtcacct tattatgatt cataatagat atttctttcg gtgaatttca
     7561 ttgtggcagc ggtaaatccc attagaatta caaaaaagac aagggtaact gccgcagcgc
     7621 gatccactct aaaatcgaca aaagctaatt catagatcca gtatacaatt acttctgttg
     7681 ctccatacgg tcctccactt gtcatgacat cgattgattg gaagactttc atcgaagcta
     7741 taaaagttgt gacaaagagg aacaaagttg taggagaaag aagcgggaga gtaatatatc
     7801 taaatcgctg gaagccattt gccccgtcga gctttgccgc ctcataatat tcaggtgata
     7861 tgcccttcat tgcagagaga tagatcatca tgccatatcc aataaccctc cagcctgtca
     7921 atatgagcac tgccgtcaag gcattttctt tagtgttgaa cattattggc ttaagaccga
     7981 taactccgag gacatagttt agaattccgt attggtcgtt caagatccac atgaatacaa
     8041 tggccgaagc cgatatggcg atatatcgcg gcataaaaac gatagagcgc aatgcagaaa
     8101 aaactttgct ctctctattg aatagggctg ctagcaacag acctccagct attgttataa
     8161 caatctccca gaatgtatag cggaaagtga tggtcaggga atttataagg ctcgcgaatc
     8221 ccgagccctt gaaaagccat ttgaaattct tgagtccaac atatttatag gtatcgctta
     8281 taaggttcca gtttgtaaaa ctgatctgaa agagttttaa gattgggtaa taaacaaata
     8341 atgcgaaaaa tatgaccgca ggtaggacgc atagaaaatc tttcaatttt tcgcgccgcc
     8401 ggtagttgat tttctttttt gtagtcattg ggttcatctc tccagcttaa ctcggaataa
     8461 ggatgtggct gacaaaaagt atcttttgtc agccacattt aaaagcgaat taatttttac
     8521 ttatcagtct tcaagagctt cgtttataag ctgtgccatc tttttcatag aagaatcgac
     8581 atctccgcct tcaataatga ccttagccaa ttcttgcatc caaatcttgg aaagcgctgc
     8641 ataggctggg tgctgaatac gtggatttat attgtttaga ttgtcgaaaa ctgccttgaa
     8701 cgctggtttt tcttccagaa atgctttggc ctcgcttgtt tgtaagacag agttgcgggt
     8761 aggcatatat ccagtttctt ttgcccaaag catgttgact tccttgcttg ttagatagga
     8821 gagaagtttc catgccgcat tctttacatc ctgtttgttc tttgacggaa taaggagaac
     8881 gcatcctcca atttccgatt tccttgtgac attgcccggc agatagtgca ttccaacttt
     8941 gaatttggca ttagtggcat acatctcgta caatgaagta gtgtgtacga ccgaaaaagc
     9001 ttttccatcg agaaagttct gtctcattat agaagaagca tcttttccat aagcccagta
     9061 agcgcttttt tcgtcacacc actgtttaag cttcttggtg attgcaacag cggttggctc
     9121 tcctagatcc gtagtattat tgtcttttat tatctttact ccagcgttta gatagaaagg
     9181 ctcgaaatac cactgatccc agcccgggat aactgttgcg tatcgttgag taactccgcc
     9241 cgagatcttc gaggctttct tcatgaattc atccatatcg gaccattttt ctggcagctt
     9301 aattttttct gcttccgcca tatctttgtt gtagaacatg atttgggttg aaataaggaa
     9361 gggcaaagaa acctgcttgc catcataact cgaggctttt cgcaaacctt caccaaagtc
     9421 atttatgtcg aatccggtag ccttgatgta tggatcaagg acctcgcaaa gccctgctgc
     9481 accatattgt gctacatagg gtgtatttgc cactgttagc gctggcaaag tagtacctgc
     9541 cgcttgagcg gctatcagct tttcgttgag atctgcatag ctgccttgat agattgcctc
     9601 gacctttata agcggattct gtttttcgaa gtcagcgatt accttgtcga taagcggctt
     9661 atattgagcc tcaagtgcgt gccaaaactg tatcttgatt ggagctttga cagcatattt
     9721 gtcagcatct tgagctccta tcccgactat aaccatcagc atcatcattg aaagaaaaaa
     9781 agtcgagata ccctttttca tcagtttcct cctcatggaa attgaaatga gatgtctata
     9841 ttaaaataga ccttgatatc actttgatta aaacactgga aatttacact gtcaacgctt
     9901 tttatttatg atttcttatg aactcagttc aaaatcatct taatgaacat atatctagac
     9961 gatcatataa ctcttttctc attccgctgc gacaggtcgg caattaagca tgcccgcact
    10021 ggacagcagc tttgtgtcgg aggataattg ctttgttctt tccattttat ttttaatcct
    10081 aaggagcacg catgaacaac gagaaatact caagaaggcg ctctgcgctt gcggcagaac
    10141 ttatgcgaag aggcttaagc gccgttatga tatcggattt cgagcatgga cgcgactcgt
    10201 cgctgcgcta tctgtgtgga cagccttcgg atgccctgtt ggtagtaaat tcgaatggta
    10261 catctgtgct tattgcctgg gatgtaaata tggcgcatcg attgggcgat gcggatcaca
    10321 tcctgccatt tactgatttt ggaaggcaat ctgctcaagc tctcaaggca gcgttcgagc
    10381 tattgggtat agagaaaggc tcgaaagttg cgcttccggc ttctactccc tatcccgaat
    10441 atatagagct tgttcagtct cttgatgctt ttgaccttgt ctgcgaaaag aacggcatcg
    10501 atggatttat caaggagatg cggtccagaa aagacgcaga cgagatcgat ttgtactgga
    10561 tgatagctaa atacaccgat gagctgatgg atatggtcga gaaggaggtt gcagctggca
    10621 ctctctctac cgaactagat gtcgctcttt ttcttgagcg cgaggcaagg cgaatgggtg
    10681 cagagggaat ggggttcgaa agtcttgtcg ccgggcctga gcgctctttt ggaatccatg
    10741 cctttcctac ttttaccgca ggtccttttg caaacaaggg gctttctatt ctcgattttg
    10801 gtctgcgctt cgagggctac actagcgatg tgactatgag ttttgtagac aatcttctta
    10861 accaggagca gaagactatg atagatcttg tcgaggaggc acatcgcatt gcggtggaag
    10921 catgcggacc aggtgtatct cttctgaagg ttgccacact tgtagatgaa ttctttgcca
    10981 atgccggatg gttcatgccg cattcgcttg ggcatgggat tggtctagat gcgcatgaac
    11041 tacctacgct aagcatgcga gccatgccag acgatctttt gctgcctggg catattgtga
    11101 ctatagagcc agggctatat catcccgagc tgggcggcgt gcgactagaa aacgatgtac
    11161 ttataaccga tacaggcgca gaagtaatta ctcactcaag aattgtgaga ttgtaggttt
    11221 ttagatcaaa cgactcgcca gctcaatctc tggctggcgc acataggcac aaggccatcg
    11281 attgtttctg gaaccgtcca aggcattcgt tggaaagcta tcgaacgcga gtcctcgtct
    11341 tgctgttcat ttcctttaac agaaggtttt gctgcaaggc catagaaacg cgcaccattc
    11401 tccgataaga aaggcacaag ttggtcaaga ccgcctaagt cagcgaaaag ttctaccagc
    11461 gcaggaaggg ctgtcggagc tgagtataca cccgaagctg ctgccgaaca ttcctttttt
    11521 cctcttgggt gcggtgctga atcgcttcca aaaaagaact tattcgaacc cgagagcact
    11581 gccatccgta gggcgtctct atcgcgggcg aacttgagga ccggcttaca atataagtga
    11641 ggattaagtg aatctgccat catgtcgtct agggtgaaaa gcagatggtg tgcggttata
    11701 gttgctgcaa ggttttgcgg tccttgttgc acaaaacgaa cactcctagc gtctgaaaga
    11761 tgctccaaga ctacatgcaa gcttggatgg cgcagaataa ggcgttcgac ctgtggcaag
    11821 aaagctcgct ctcgctctaa aataggagcg cttggatctt ccccatggat gcacaaaacg
    11881 agccccattt cttccatgac attcaatgca tcttccactt cgtcgaagga tcgaggacca
    11941 tccgcggcat tggttgtcga tcccgaaggg taatacttgc cggctatggc tcctttttga
    12001 accaatgctt cgatatcata acctttcatt ccaggaagca gcttgaaagt gtagagcggc
    12061 tcaaagcgct tttccaaagg aagctctgcc aggacttggt cgacctcgct cctataggcc
    12121 ttgagccggc tcgggctggc tacagggggc agggtattgg gcattaggag cactctggca
    12181 aattgaccgg catgtccgag caccgaggcc ttaagtagag ctccttgccg caaatgagca
    12241 tgaaagtcgt cgggcaaggg catatcgaat ctatcgatgt ccgctttgtc ctttgtgatc
    12301 tctttggcca tgatgtcctc cagaaaaacc tcagtgcgct tgacctgcct atcggcagcg
    12361 catacagtaa ggctatggac aaatatatca tcgaaggtag atatcctatc aagggaacac
    12421 tcacactttc aggcaataaa aacgccgctc taccatgtat tgcggcctca gttttagccc
    12481 aggagcctgt aaccctccga aacatacccg caatcgagga tgtcgctgtt atgttcgatg
    12541 tctttcgata tctgggtggc ctagtcgagc aaatcgatac tcatagttgg tgcttagatc
    12601 cacgttctat ctcgaaatgc gaagtacccg aggagctcgc caagaaggtc agagcctcca
    12661 ttctatttgc tggtcctctt gtagcgcgct ttggtaaagc aattctgcct cctccgggtg
    12721 gagacacgat agggcgtagg cgcatagata cacatattgt tgcgcttcaa gaacttgggg
    12781 cacgtatctc gctagaaaat acgcttggtt tttctgcgac caaactggtg ggtgtgccgg
    12841 tttttttgga tgaagcatcg gtgactggca cggagaatgc gataatggcg gcatctcttg
    12901 cagagggaac tacaattctt atgaatgccg cctgtgagcc gcacgtgcag gatctctgca
    12961 gaatgctcat ccagatgggc gctgatatcg atggagtagg atcgaaccgc ctcattatcc
    13021 aaggtgcaaa atccctaaat ggcacggatt tcacaatcag cgcagactat atggaacttg
    13081 gctcttttat tggccttgcg gcagtgacag gcggagatct gcggatcgat ggagtcaggg
    13141 cggacgatct tccacctctc aaggtggggt tttctaaatt aggcgtaaca tgggatttga
    13201 aggggaacac attgaggcca aataacatgg tcgccctgcg tgttgttccg gatataggtg
    13261 gccaaattcc taagatagac gatgccccat ggcctggatt tcctgccgac ttgacctcga
    13321 taatgacggt agtggcaacg caagcagatg gggtagcgct tatccatgag aagatgttcg
    13381 agtcgagaat gttctttgtg gataaattga tatcgatggg cgcaagaata gtgctatgcg
    13441 atccgcatag agccattgtc tatgggccat cgaagcttgt gggcaatgtg atggtgtcac
    13501 ctgatgtgcg agcaggaatg gctatggtta tcgctgctct ctgcgcagaa ggcactagca
    13561 ttatccacaa tgtctaccag attgagcgtg gctatgagaa tctggtcgag agattatccg
    13621 cagttggggc tcacattaag cgagaataaa aatagctgac tgaaaaccga tgtaacacaa
    13681 ataaagaaaa aggcccaatc aaaaagaaga ccaatagaag aaggactaaa aagtcttatc
    13741 actgctgaga attagcccgc cccgcgtcct cggttagcat ctttttcttg agctcttcga
    13801 gcataacatc cgctgcctgt ggcgaggatt ctagctgagc aaattgcttt tctaggcttg
    13861 cgcctgtggt agtatcctct agttccttcg tagcctctgc ttgtgcttcg acttcctcga
    13921 ttttgcgctc catgcgctcg aatgtctcga aggcgctctt attgcctgcc atgctcgaca
    13981 tggtctgagt tatgcgctgt tgagcctcgg cccgctttgc tcgtgctata agaatgtttt
    14041 tcttgcggtt tgcttcttcg attttgcttt gcagctggcg cagagcttct ttgagctttt
    14101 ctacagactg tttttgtgct tcccactgtt gagcgagctg ggtagcatag ttttcgaatt
    14161 cttgctttcg aaggagagct tccttggcga gatcatcgcg gttagcgcgg accgcaagca
    14221 tggctttctt ttcccattct tgtgccttgg ccttgttctc catcatttct cgctctagcc
    14281 gcttctcgtc tgcaatggcc atagcaacag ctttcttgga ctcgataagt tgctcgttca
    14341 tgtcgatgat catctgattg agcatttttt cggggtcttc ggctttggag atgatagcgt
    14401 tgatattcga tgagatcact gttttaaggc gatcgaacat tcccatagtt cattcctcca
    14461 ctttatgaat atagttcgca agcttcttat agtgctcgag caatgcgaac gatatcgatt
    14521 caatagatgc aagaaattct tctttgtcaa gcgtgttata ctcgagcgta tctatcacga
    14581 tgatttggtc gccgcttatt gcataagcgc catgaagaag atccgtagca ttgagctcta
    14641 ggagcgtcct gaagagcgct tcggcgttct cttttggtat tggcattaca tttgcgctga
    14701 taataacaag aggatccgaa aggctgacta tcattctggg gtagcctttg ctttcatcct
    14761 caattagcca ggaactgttg gaaagctcct gatattcgat tccgaggtcg atgagatagc
    14821 tttctattct ggtcaatcct gacattacta cctcctcctt gtacttctat tgtcttagat
    14881 ctatgcgcta tgcgctcaac cgagcgttat agttccatcc gggaatgccg ttattactgc
    14941 ttcgcaggtc tgacaccgcc ccctaccagg cttttctata tctataagag agttgcaagc
    15001 tgggcactgg acagataatg gatatatgcc atttattgcg aatttaagac ctgtcagcgc
    15061 gctttgggca tccactgcgg tcgtaaaata gccctcgagt ccgagtatcc tctgcattcg
    15121 ttccatgcaa ggccgcatgc cacagagcac gaggtcgcca tgccggtctc tcaatttttg
    15181 cctgagcgca agcattagcc ccattcctgc tggtccgata tcttcaatag tagaaaaatc
    15241 gaccaccaga tagcgtatgc cttcggagag tatgctatcg aatgattgag caaccgtatc
    15301 cagattagct tctgtaaccg cacctacaaa gcgtgcgacg atgcagcctg ggaaatcagt
    15361 agatatctgg gacgagatta aggcatctga acctggttga gaaatcttct ggattcgcga
    15421 aagaagtccc gatggcatga tctatcaata atatgagtta ggctaggaaa agtcaaacac
    15481 ccctagaagg aatgctgcct aactagcttt acacagaatt gtcgtgcacc ctcaaacacc
    15541 cgctaaca
//
LOCUS       DAOSOB010000049        15340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_3879_length_15340_cov_70.829310, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000049 DAOSOB010000000
VERSION     DAOSOB010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 15340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15340
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3879_length_15340_cov_70.829310"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /gene="dctP"
                     /locus_tag="PLS88_06245"
     CDS             <1..439
                     /gene="dctP"
                     /locus_tag="PLS88_06245"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOU06976.1"
                     /translation="AVPVAMAYGEMYSALQTKAIDGLELSFTATASGKFQEVAKYACE
                     TKHILLVNFEVVSSKWFNSLPLEYQKILQEECDKAGLEVSRRYLEEIDPKNIEVLKAG
                     GMTIIPASEIDMAAFKAAGEKAYQKLDLQAVRDQVYKELGKTK"
     gene            538..1008
                     /locus_tag="PLS88_06250"
     CDS             538..1008
                     /locus_tag="PLS88_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOU06977.1"
                     /translation="MKKVYQFIGKAEVIISATCFTISLLLIFSGAIARTLRHPLNWSQ
                     DLSLFLFAWGVFLSADAAMRANKLVNINLFINRLPENMRKIFATVVYVIIFIFLIFLS
                     YNGIKLSVFSRQRVFQGIPGFSYSWVMLSIPVGSALQMITIILKIKNLYKREEH"
     gene            1010..>1911
                     /locus_tag="PLS88_06255"
     CDS             1010..>1911
                     /locus_tag="PLS88_06255"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOU06978.1"
                     /translation="MLIVGILLLVFLAIGMPIAYAIGISGFAFFLQHPELPMTIIAQL
                     PISQTQNFTLLAVPLFILAGNLMNASGITSRLIKLSSVLTGHMRGGLAQVNVMLSTLM
                     GGVSGSAIADASMEARILCPAMIERGYSPGFTANCTAWTGLITATIPPGVGIILYGVT
                     GEVSIGRLFAAGIFVGIFLTVVYMFTVWVISGREGYKAERARASFRECMQAIVENIWA
                     LLFPIFLLVGLRIGIFTPSEVGSFACVYAILVGIFAYKELTLKKLVQTLADSVLDVGG
                     IMFIIALSGIFGYGIPFEKIPDLIS"
     assembly_gap    1912..2011
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2077..2817
                     /locus_tag="PLS88_06260"
     CDS             2077..2817
                     /locus_tag="PLS88_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU06979.1"
                     /translation="MELQGKIAIITGSARGIGFEIAKTLAREGAFSVIVDRSGAEAAA
                     ISLQEKQYGAMAVSLDITDIAAVNNAVKTIISAKGRIDILVNNAGIIARGSIVDLSRE
                     QWLQVMDVNVNGNFYFCKAIIPYMIRQHSGNIVNVTSIAGKMGDITAAPVYGTSKGAI
                     NAFTKSLARQLAKNGIRVNAVAPHAIETSMSAEWTIEKRKEVIDAIPLKRMGKPEEVA
                     EAVLFLASERSSFITGEILNVNGGMLMD"
     gene            complement(2830..3534)
                     /locus_tag="PLS88_06265"
     CDS             complement(2830..3534)
                     /locus_tag="PLS88_06265"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU06980.1"
                     /translation="MEESDNIIIEKRSLAEQVYLYLCNSISNGKLKYGETINTRRLAE
                     ELNISMMPVREALKRLETDGIVEIKPRSMCLLRTPTRETILDAIAAREMLEIYSVKTI
                     YASIENPSLDVLRKIIEEMKTAISEDPIDLRKYIKHDWQFHKELCNLVGNQFVSKFYK
                     ELNIHLNMSYVYDIGIEPNVSQTFRDHIDLVDALAAHSSEAVKIIERHLRINRQNILS
                     GRFFSSQKEERSEQTV"
     gene            3785..5176
                     /locus_tag="PLS88_06270"
     CDS             3785..5176
                     /locus_tag="PLS88_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU06981.1"
                     /translation="MKFAFLHQSTELRRDYILNGPISSTLMLLAAPTLMLGVVQALMP
                     LMDGLFVNNIAGTLVASSVTFSEPVINMTTALAQGLSVAAMAIIGQLNGRGDLEESKR
                     TSTQIVVMGSILGCISAPFLVLAAKIISSKVNPSISHNVFLYLSLYALVLPFSFMESI
                     YNGIKNANGIPEAPFIRMVLMLILKIAFNFIYIYLLRLEIVGCVLSSLSANIIITVWM
                     FWELFLKKGPDKLELKGFKFDPAAAKQLFRIGAPAMANTFILNLGFFLINTEVEKYGP
                     VVLNGQGIANNITQVAFNVPSAFGSAVTTMVSMNIGAENPRKAKESMVHGTSLSALTA
                     AILIAIIVPLSSRLTVLFTRRPDVLEIANRALHIYTYSVIGFGITTTIQGAFIGLGRT
                     KVPLILGILRIWLLRYVFILLTEKYLQYYAVFWGNLFSNYASALIAIVLISKTKWESA
                     IKPSTQYSIASAK"
     gene            complement(5256..6047)
                     /locus_tag="PLS88_06275"
     CDS             complement(5256..6047)
                     /locus_tag="PLS88_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU06982.1"
                     /translation="MICVDDIVVSFDLGGGQEHTVLNRLSLEVKQGQTVSIIGSNGAG
                     KSTLLNAIAGSIPLRAGQIRLNGEDVTSWPEWERAMYLGRVRQDPMAGTASEMTVLDN
                     LALAARKGPRKFRIATPPRFAREMEKKLAELGMGLESRLRENVSRLSGGQRQALTLLM
                     AVLSHPTVLLLDEHTAALDPANAEMVDALTRRFIDEYSLTALIITHDMKRALEQASRV
                     IMMHEGEIIADLSGPEKEKMNVAALVRLFKKVRGSEYAEDRDLLI"
     gene            complement(6064..6972)
                     /locus_tag="PLS88_06280"
     CDS             complement(6064..6972)
                     /locus_tag="PLS88_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU06983.1"
                     /translation="MVEGILVEGLIYGILALGVFVSFRILDFPDLTVEGSFPAGAAAG
                     AVVASALNISPNVVLRALAVPAGMLAGFVTGGVAGFATAAVYHKLRVPPLLAGIVTMT
                     GFYSINLRILGGKPNYPLITNNPMLKLARSFLGGALPSEWSLFASCVLVCLLLFGLLD
                     FFFHTEIGIAMGALGDNENAVVQAGIQPNRLRTWGIMLANALPGLSGAMAAAYQGFAD
                     VNLGQGVVAAGLATVMLGELIIHSQFISVQLLRVFLGSILFRALMYAARSWGYVAGIT
                     PNDLRLLTALLIIASVALSRVKRKRS"
     gene            complement(6976..7965)
                     /locus_tag="PLS88_06285"
     CDS             complement(6976..7965)
                     /locus_tag="PLS88_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU06984.1"
                     /translation="MSSRSAKLLYTIGCLGILLLSCAPKSAKVIGVAKFVSHPALDAI
                     EKGILDELSASKPDYKVDLQNANADMNTAAQIAQHFKQEKVALAVGIATPMAQALANQ
                     IKDRPVIYSAVTDPVAADLVESMEKGGPNITGTSDMTPVREQLDLLCSLKPSIKRIGN
                     IYASGEANSVALAAIVRDYCQEHSLEYIESTITNSSEARQALLSIAGRIDGLYLGNDN
                     TVFSALSGIAEVGIEKKIPIVTADPSSAETIPVLAALGYDYYRMGVATGKIVVRILNG
                     EKTANIPAFLPKESEDMTFVLNLDTARKIGISVDQSIIDRAKVVISDDQLVRK"
     gene            8357..9475
                     /locus_tag="PLS88_06290"
     CDS             8357..9475
                     /locus_tag="PLS88_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU06985.1"
                     /translation="MKQRKMVFATIVSLVLFFGAQAVFAAPTIKIGVAGAHSGDLASY
                     GLPTVNAAKLVVKEYNDKGGINGQKVELVIEDDQCKPEIATNVAAKLVSTKVVAVIGH
                     ICSGATKAAMGIYKDSKLVCISPSATTPSLTQSGEYPNFFRTIAPDDAQARLATDFLV
                     NDLKVKKIAVLHDKGDYGKGFAESVKEFAEAKGIQVALFEGINPGAPDYSAVVNKVGN
                     AKVDAVVWGGYHPEASKLVQQMKEKGINVAFISEDGIKDNTFIEVAGKYAEGVYATGP
                     TDTSSNPMATKAIEAHKKEFGSEPGAFFLNAYAAAQALLNAIAKAKSTDYDKIVAALQ
                     SEYVDTVLGRITFDKKGDIVGYGNSLFQVQNGKFVVLK"
     gene            9566..10468
                     /locus_tag="PLS88_06295"
     CDS             9566..10468
                     /locus_tag="PLS88_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967890.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HOU06986.1"
                     /translation="MEYFIKLFLSGTAKGSIYALMALGYTMVYGIIQLINFAHGEIYM
                     IGGFTALILGGFFFSKGMPVSLVLVFSILLSIMYAAAFGFTIEKIAYKPLREKPRLSA
                     LISAIGVSIILQNFVLLSQTEKFLPFPNYLPEFAWLLPYKEYLNSTQLIILITTAIIM
                     VLLTLLIKFTRMGKAMRATAQDMHMAQLVGVDINQVISLTFVIGSALAAIGGVLICSY
                     MGQINYYIGFIAGIKAFVAAVLGGIGSIPGAVLGSFVLGWTESFGTGYVSSDYEDAFA
                     FLILIIILLIKPDGILGRTRRQKV"
     gene            10472..11743
                     /locus_tag="PLS88_06300"
     CDS             10472..11743
                     /locus_tag="PLS88_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOU06987.1"
                     /translation="MKASSILKEIGKSLIIAIGCMVLFFPLMVMKVNVTRGVAHINFR
                     WNVLPLIGIVAFVLSFVWSKALEWNEKRASAKKVENGLDNNRALNGRYLKNPALRPIF
                     LFALFIFAVAFPFLFGMYHTNVMITAFIYVILGLGLNIIVGLGGLLNLGYAAFFGIGA
                     YTYGLIWKYIGPSFVAAGFNPGWLFWISLPLAGIAATVFGIILSLPVLRLRGDYLAIV
                     TLAFGEIFHMVMQNSGDITGGATGISLIPRPWFLGQKLRPPEAATYIYFIALVFVIIT
                     IFVVRRIEDSRVGRALEAMREDEVACQAMGIDMVKNKLITFAVGTFWAGIAGVLMASQ
                     TTYINPDSFTLWESIMILMAVVIGGTGSIPGVIAGAILLKLLPEYFRPLAQYRMLIYG
                     IAMILVIIFKSDGLIPRKRKQYTFKEKELAK"
     gene            11740..12540
                     /locus_tag="PLS88_06305"
     CDS             11740..12540
                     /locus_tag="PLS88_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06988.1"
                     /translation="MSTEKLQYHHEILEIDNLVMTFGGLRAIDGVSMHIDEGEIAALI
                     GPNGAGKTTIFNCITGVHKPTEGKVKIRNRDGRAEEIQGLKPNLINRKGLARTFQNIR
                     LFNNMTVLENVMIGRHNSLKAGVFAAIIRNAPTKEEEQRVIEESYTILKKLKLDAYVN
                     EMAKNLPYGEQRRLEIARALATDPFLLLLDEPVAGMNAQETKELEETINIIRDQEHIT
                     ILLIEHDMSLVMDVSERIYVLDYGKLIAEGSPHEIKKNPDVIKAYLGE"
     gene            12542..13255
                     /locus_tag="PLS88_06310"
     CDS             12542..13255
                     /locus_tag="PLS88_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU06989.1"
                     /translation="MALLELKDVRTFYGNIQALKGVSISVEEGEIVTLIGANGAGKTT
                     TLMSICGITPIRSGEIFLDGRDISRMSPHKIVQMGVSQVPEGRRIFPQLTVSENLDMG
                     AFLRNDKESIKQDMEEVFTIFPRLAERRNQLGGTLSGGEQQMLAISRALMASPRLLLL
                     DEPSLGLAPLIVQNIFEVIQKINKERKTTILLVEQNANMALKIANKGYVMQNGLIKIT
                     DTATNLLKNQEVRKAYLGL"
     gene            13340..13984
                     /locus_tag="PLS88_06315"
     CDS             13340..13984
                     /locus_tag="PLS88_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU06990.1"
                     /translation="MKVGQRMTRNPVTITPDTTVPEAQAIMRREKIRRLPVLDKNGKL
                     VGIVSALDLMHASPSPATTLDVWEMHYLLSKLTVDKVMTKDVITITEDLPIEEAARIM
                     DDNQISGIPVMRGEILVGIITESDIFRLFIELFGARHKGIRLTLLLPEKRGELAKLSN
                     AITKCGGNIVSFATFEGEDPTNAYCTVKVTGVEKEALIQAITPVVEKVVDVRET"
     gene            complement(14020..14448)
                     /locus_tag="PLS88_06320"
     CDS             complement(14020..14448)
                     /locus_tag="PLS88_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOU06991.1"
                     /translation="MSERKGYIRPSPIEADIDTQFEALKKAGAEKIFEEKPLSKEADR
                     PELRRALDSLCSGDTLIVASLDRLARTVTELFYILEEIRRKGAMLESLKEHLEIGDDF
                     MRHLGIIIDFERSSLMEWQRETIEAKKSRQEKGTFSRRQR"
     gene            complement(14507..>15340)
                     /locus_tag="PLS88_06325"
     CDS             complement(14507..>15340)
                     /locus_tag="PLS88_06325"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOU06992.1"
                     /translation="TQLPTPFARWFEDELRLALGRTSKMKLFDKQVAAAMDPAIREQY
                     AVFFGQDRADSILYGKYIRDGDGVLTTLSLTDLATGALISETRYLVKKGILPADVSVE
                     PSIKALETAVSLSQLVPGSVSQVSGSPSLGSQAYAMSDFQLSLSTDRGVGAVYRNGER
                     LKLYLTSNKDAYLKIYHVDVNGVAQLIWPNRFGGSGKIRAGEAMQFPGPNDKFQYLLG
                     PPYGTEYIKAVASTKPFTTMEADFSDLQGSAAAAITRGLSVVSSDATRSEALVVYEIV
                     P"
BASE COUNT         4090 a         3563 c         3428 g         4159 t
ORIGIN      
        1 agctgttccg gttgcaatgg cttatggcga aatgtactcg gctttgcaga caaaggcgat
       61 cgatggcctc gaattatcct ttactgctac cgcgagcggc aaattccagg aagtggcgaa
      121 atatgcctgc gagacaaagc acatccttct cgtgaatttc gaagtagtat ctagcaaatg
      181 gttcaactct ctgcctttgg aatatcagaa aatcctacaa gaagagtgcg ataaggcagg
      241 actggaggtc tcccggcgct atctcgaaga gatcgatccg aaaaatattg aggtactaaa
      301 agctggcggt atgactatta ttcccgcaag tgaaatagac atggctgcct ttaaggctgc
      361 aggtgaaaaa gcctatcaaa aactcgacct gcaagcggtg cgagaccaag tctataagga
      421 acttggcaag acaaaataat ctcgtcaaag cagcgctgcc tgcctacaag gcagcgctgt
      481 ctcaaaggcg cgttgcattt tcttttcata ttcagatgca catcaggagc tgttcacatg
      541 aaaaaggttt atcaatttat tggaaaggct gaggtcataa tttcagcgac atgcttcacc
      601 atctcgcttt tgctcatatt ttctggtgct atcgcaagga ccttaagaca tcctctaaac
      661 tggtctcaag atttgtcgct ttttcttttc gcatggggag tttttctgag cgcagatgca
      721 gcaatgagag ccaataagct tgtaaacatc aatctgttca taaaccgctt accagaaaat
      781 atgcgaaaaa tttttgccac agttgtatat gttatcattt tcattttctt gatttttcta
      841 tcctacaatg gcatcaaact gagcgttttt tctcgtcaaa gagtgtttca aggcattcca
      901 ggctttagtt actcctgggt catgttgagc attcctgttg gatctgcgtt gcagatgatc
      961 actatcattc tcaagataaa aaatctctat aagagggaag agcactgata tgttaatcgt
     1021 cggaatcttg ctattggtct ttcttgcaat cggaatgcct atcgcttatg caatcggcat
     1081 atctggcttt gctttctttc ttcagcaccc cgagctcccc atgaccatta ttgcccagtt
     1141 gccgatatct cagacgcaga attttacatt gcttgcggtt ccgctcttca tccttgcagg
     1201 taatctcatg aatgcttcgg gcataacgag caggcttata aagttgtctt cggtattgac
     1261 cggacatatg cgaggaggtc tcgctcaggt caatgtaatg ctgtcgactc tcatgggagg
     1321 cgtatcgggc tcggccatag cggatgcatc aatggaagcg cgcattctct gccctgcaat
     1381 gatagaaaga ggatattcgc ccggttttac ggcaaactgc acggcttgga caggccttat
     1441 taccgcaacg attccgcctg gcgttggtat tatcctctat ggtgtaacag gagaagtctc
     1501 tattggaagg ctttttgccg cgggaatatt tgtagggatc tttctcactg tggtatatat
     1561 gttcaccgta tgggtcattt cgggacgtga aggttacaag gctgaacgcg ctcgagcttc
     1621 atttcgagaa tgcatgcagg ctattgtgga gaacatctgg gccctgctct tcccaatatt
     1681 ccttcttgta ggattgagaa tcggaatatt taccccatcg gaagtaggct cttttgcttg
     1741 tgtctatgcg attctggtcg ggatattcgc ttataaggaa ctaactctaa agaagctggt
     1801 gcaaacactt gccgatagtg tgctggatgt cggaggtatc atgtttatca ttgcactcag
     1861 tggtatcttt ggttatggaa ttccgttcga gaaaatcccc gaccttattt cnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntagttttct tccctggctt agtacttgca
     2041 ttaccaagac tgcttttctg agatcgagag gataacatgg aactacaagg gaaaatcgct
     2101 ataattactg gttcagcaag aggaattggc tttgagatcg ccaagactct tgcccgtgaa
     2161 ggagccttct ctgttattgt agatcgaagt ggcgcggaag ctgcagcaat atccctgcaa
     2221 gaaaagcaat atggcgcgat ggctgtttcg ctcgacataa cagatatagc tgcggtgaac
     2281 aatgctgtaa agacaattat aagcgcaaaa ggccgaatcg atattctcgt caacaatgct
     2341 ggtataattg cgcgaggcag tatcgtcgat ctaagccgag agcaatggct gcaagttatg
     2401 gatgttaatg tcaatggaaa cttctatttc tgcaaggcaa tcatccctta tatgattcga
     2461 cagcattcgg gaaacattgt gaatgtaact tcaattgcgg ggaagatggg cgatatcaca
     2521 gccgctccgg tttacggaac ctccaaggga gctatcaatg cttttacaaa atcgcttgct
     2581 cgccagcttg caaaaaatgg catacgggtc aatgctgttg ctcctcatgc tatcgaaaca
     2641 agcatgagtg cagaatggac aatagaaaaa aggaaagaag tcatagatgc aattccatta
     2701 aagagaatgg gcaaacccga agaggtcgct gaggctgttc tcttccttgc ttcagagcgg
     2761 tcttctttta taacaggcga aatcttgaat gttaatggcg ggatgctgat ggactaaaaa
     2821 gctccgaatc tagaccgttt gctcgctacg ctcttctttt tgcgaggaaa agaatcgacc
     2881 gcttaaaatg ttctgcctgt ttatgcgcaa atgccgttcg attatcttta ccgcttccga
     2941 agaatgggct gccaatgcat ccaccagatc gatgtgatct ctaaaagtct gagagacatt
     3001 cggttctatg ccgatatcgt atacataact catattgaga tgtatattca attctttata
     3061 gaacttactg acaaactgat tcccgaccag attgcagagc tctttgtgga attgccaatc
     3121 atgcttgatg tatttcctaa gatcgatcgg atcttctgag atagctgtct tcatttcttc
     3181 tattattttt cttagcacat cgaggctggg gttctctatg gaggcatata tagtctttac
     3241 actatatatc tcgagcatct ctcgggcagc gatcgcgtcc aatatcgttt ctcttgttgg
     3301 tgttctgaga aggcacatag aacgaggctt tatttccaca atgccgtctg tttcgagcct
     3361 cttgagcgcc tctcttactg gcatcatgct gatattcagc tcttcggcca gacgtctagt
     3421 attgattgtt tcaccatatt tcagttttcc attgctgatc gaattgcaca gataaagata
     3481 gacttgctcc gccaggctcc tcttttcgat gatgatattg tcggattctt ccattttcga
     3541 ttcctttccc agtgatcttt ctctactaaa tcagaataca tcaattctct tttgaaatca
     3601 ataatatcag aagctgatat ctattatctt tttaatttcg atttattgta ctgggcggtg
     3661 tgtcttgcag cctatcgatc cggctgtgcg accgatctgg ctgtgcgatc tgctttacag
     3721 tactgttatt ccttataatc tcttgattag attttaaaaa gagaatatat atctttcata
     3781 atctatgaaa tttgccttct tgcatcagtc gaccgaattg cgacgtgact acattctaaa
     3841 tggccctatt tcctctactt tgatgcttct cgcagcgcct acactcatgc ttggtgtcgt
     3901 tcaggcactc atgcctctca tggatgggct gttcgtaaac aatattgcag ggactcttgt
     3961 ggcaagctcg gttaccttca gcgagccagt gatcaatatg acaacagcgc ttgcacaggg
     4021 attgagtgtg gctgcaatgg ctatcattgg acagctcaat ggacgcggtg atctcgagga
     4081 atcaaaacgc acttcgacac aaattgtggt catgggctcg atacttggtt gcatttctgc
     4141 accttttctt gtcttagccg ccaagataat ctcatcgaaa gtcaatccta gtatttctca
     4201 caatgtcttt ttatatcttt cgctctatgc tcttgtgctt ccattttctt ttatggaatc
     4261 tatttacaat ggtattaaga acgcgaacgg catacccgag gcaccattca taagaatggt
     4321 actcatgctc attctcaaga ttgccttcaa cttcatatat atctatttat tgcgtttaga
     4381 gattgtaggt tgcgtcctat cttcgctctc cgctaatatc attatcactg tatggatgtt
     4441 ttgggagttg tttctaaaaa aggggccgga taagctagag ctaaaaggat tcaagttcga
     4501 tccagcggcc gccaaacagc tatttcgcat aggtgcccct gcaatggcaa acacctttat
     4561 actcaatctc ggttttttcc ttatcaacac cgaagtagaa aaatatggac ctgtagtact
     4621 caatggacaa ggaatagcaa acaatataac tcaagtggcc ttcaatgttc ctagtgcatt
     4681 tggttcggca gttacaacca tggtcagcat gaatattggc gcggagaacc cgcgaaaggc
     4741 caaggaatcc atggttcatg gcacatctct aagcgcctta acggcagcaa tacttatcgc
     4801 tatcatagtc cctctctcgt cgcggcttac tgtcctcttt acaagaaggc cagatgttct
     4861 cgaaattgca aacagggctt tgcatatcta cacttattcg gtcattggtt ttggaataac
     4921 gactacaatt cagggagctt tcataggact tgggcgcacc aaagtacccc ttatcctggg
     4981 aattctgcga atctggcttc tgcgctatgt ttttattctc ttgacagaga aatacctcca
     5041 gtattacgcg gttttctggg gaaacttgtt ttcgaactat gcaagcgcac ttatagccat
     5101 tgttttgata tcaaagacta aatgggaatc ggcaatcaaa ccgtcaacac aatatagtat
     5161 cgccagtgca aaatagccag cgcagaatag ctggtgcaaa ctgctggcag ctttggaaaa
     5221 tactttgact atctggcata aagctcaaaa aaatttcaaa tcaagagatc cctatcctcg
     5281 gcatactccg atccacgtac tttcttgaat agacgaacaa gcgcggcaac attcattttc
     5341 tccttttctg gaccgcttag gtcggctatg atctcacctt catgcatcat tatcactcgg
     5401 ctcgcttgct cgagggcgcg cttcatatcg tgagtgatta ttagcgccgt gaggctatat
     5461 tcatcgataa agcggcgcgt aagagcatcc accatctccg cattcgccgg gtcgagcgcg
     5521 gctgtatgct catccagcaa taataccgtt ggatgagaga gtacggccat caaaagcgta
     5581 agcgcttgtc gctggccacc agacagcctt gaaacattct ctcgaaggcg gctttcgaga
     5641 cccatgccta gctctgcaag ctttttctcc atttctcgag caaatcgtgg aggtgtcgct
     5701 atgcgaaatt tgcgaggccc tttgcgagcc gcgagcgcta gattgtcgag aactgtcatt
     5761 tcgctagcgg taccagccat aggatcttgg cgaactcgac ctaaatacat agcgcgctcc
     5821 cattctggcc aagaagtcac atcctcgcca tttagacgaa tttgcccagc gcgcaatgga
     5881 atgcttcccg ctatagcgtt gaggagtgtg cttttgcctg ctccattcga accgatgatc
     5941 gatactgtct gaccttgctt tacctctagc gaaagcctgt tgagaaccgt atgttcctgc
     6001 cctcctccca aatcgaagct tactacaata tcatctacgc aaatcatagg agcatccttt
     6061 ctttcatgag cgcttccttt tgactcgaga aagagccacg gaagctatta taagaagcgc
     6121 agtcaaaagc cttagatcgt tgggggtaat accagcaaca tagccccagg agcgagcggc
     6181 gtacatcaat gctctaaaca agatcgaacc aaggaaaact ctaagaagct gaacacttat
     6241 aaattgcgaa tggataatga gttcgccgag catgacagta gcaaggcctg cagctaccac
     6301 tccctgccca agattgacat cggcaaagcc ctgatacgcg gccgccattg cgccagaaag
     6361 acctggtaat gcattggcga gcattattcc ccaagttctc aatctatttg gctggatgcc
     6421 cgcctgaact acggcatttt cattgtctcc aagcgcaccc atagcaatgc caatctcagt
     6481 atgaaagaaa aaatcgagaa ggccaaatag caaaaggcag accagtacac aagaggcaaa
     6541 gagcgaccac tccgacggta gcgcgcctcc aagaaaggat cgcgctagct tgagcatggg
     6601 attgttggtt atcagtggat agttcggctt tccaccaaga atccgcaaat ttatcgagta
     6661 aaaccccgtc atagtgacga tacctgcaag aagaggcgga actcttagct tgtgatagac
     6721 agccgccgtg gcaaagcccg ctacccctcc tgtgacaaag cctgccagca ttcctgcagg
     6781 tacggctagg gcacgcaaaa caacatttgg cgatatatta agtgccgaag ctactactgc
     6841 ccctgccgca gctcccgcag gaaaggatcc ctctactgtc aaatccggga aatccagaat
     6901 tctgaagctt acaaagaccc cgagcgcaag aatgccatag atgagccctt caacgagtat
     6961 tccttcaacc atcgattact tccgcacaag ttgatcatcc gaaatgacta ctttcgccct
     7021 atcgattata gactgatcga ccgatatgcc gatttttctt gcggtatcga gatttagtac
     7081 gaaagtcatg tcttcgctct cctttggcag aaatgcgggg atattcgcgg ttttttctcc
     7141 atttaggatg cgcactacga ttttgcctgt tgcaacaccc atacgataat agtcatatcc
     7201 tagagctgca agcaccggta ttgtctccgc ggaagaggga tctgcagtta caataggaat
     7261 ctttttctca attcctactt ccgcaattcc gctaagtgcc gagaaaacgg tgttgtcatt
     7321 acctaggtag agcccatcga ttcgccctgc aatagagaga agcgcttgtc tagcctcgga
     7381 ggaattcgtg atagtggact ctatatattc gagactatgt tcttgacaat agtctctgac
     7441 tatcgcagct aaagccacag agttcgcctc gcccgaagca tagatgtttc caatgcgctt
     7501 tatgcttggc ttcaatgaac agagaagatc caattgttcc cgcacaggag tcatatcaga
     7561 tgtgcctgta atgttgggtc cacccttctc catgctttct acgagatctg ctgcgacagg
     7621 atccgtcacg gcggaataga tgacaggccg atccttgatc tggtttgcta gcgcctgagc
     7681 catcggtgtc gcaattccaa cggcgagtgc aaccttctcc tgcttgaaat gctgtgcgat
     7741 ctgggcagcg gtgttcatat ccgcatttgc attctgcaga tcgaccttat aatcaggctt
     7801 agatgcggag agctcgtcta agatgccctt ttctattgca tcgagggccg gatgcgatac
     7861 gaatttcgct accccaatga cttttgcact ctttggcgca caagataaga gcaagatccc
     7921 taagcatcca attgtataga gcaacttggc agaacgacta ctcatacaaa ctctccttaa
     7981 tgaaatgaaa tgatttttac atgcctgagc catcggcgct tgcatgttcc atgaaaacta
     8041 ttgttactat gcatagtaac attttcggca ttgtatcttc tgctgtaaac ttcgtcaata
     8101 tacaaaatga agaaaattca ataattttta gaaaattttt ctttaggtta ctttgaggat
     8161 gtcctcactg gatagcattt caaattttga gacaagatgc gcaaagatta aaatattatg
     8221 ccttttagaa ataaaaagct ttacttttga tagaaacacc ttgacacgga gttttaggat
     8281 atatattctt cgcagaaatt ggcttcattc cattatttat gcgcatgttt tgtatatgcg
     8341 gaaggaggaa ttcaaaatga agcaaaggaa aatggttttt gcaaccatag tgagccttgt
     8401 gctcttcttt ggtgcacagg ctgtatttgc ggcgccaacc ataaagattg gtgtcgccgg
     8461 cgcacactct ggcgacctgg cttcctatgg cctgcctact gtcaatgcag ccaagctggt
     8521 tgtcaaggaa tacaatgaca agggcggcat caatggccaa aaggttgaac tcgtcatcga
     8581 ggatgatcaa tgcaaaccag aaattgcaac caatgtcgct gccaagctgg taagcacaaa
     8641 ggtcgttgcg gtcatcggac atatttgttc gggagctaca aaagcagcta tgggaattta
     8701 taaagattcc aaattagtct gtatctcgcc atcagcaaca acgccttctc ttacccagag
     8761 cggtgaatat ccaaacttct tccgcacaat agctccagat gacgcacagg caaggcttgc
     8821 aacggatttc ctcgtaaatg atctcaaagt aaagaaaatc gcagtgctcc atgataaggg
     8881 cgactatggc aagggattcg ccgaatctgt gaaagagttc gccgaggcaa aaggcattca
     8941 ggtagctctc tttgagggca taaatccagg cgcccctgac tactccgctg tcgtcaataa
     9001 agtcggcaac gccaaagtcg atgccgtggt atggggcggc tatcaccctg aagcatccaa
     9061 actcgttcag cagatgaaag aaaaaggaat aaatgtcgcc ttcatttcag aagatggcat
     9121 caaggacaac accttcatcg aagtggctgg aaaatatgcg gaaggcgtct atgccactgg
     9181 cccgaccgat acctcttcta atccaatggc taccaaggcg atcgaagctc acaagaaaga
     9241 gttcggttct gagccaggcg cattcttctt gaatgcctac gccgcagccc aggcactgct
     9301 caatgccatc gcaaaagcca aatccacgga ttacgataag attgtggcag ccttgcagtc
     9361 ggaatatgta gacacggtgc ttggaaggat tacattcgat aagaagggag atatagtagg
     9421 ctatggaaac tctctcttcc aagttcagaa tggcaagttt gtagtattaa agtaaagatc
     9481 gtgtccttct gagcctccag aagccgttct ggccctaaaa gtctgaacgg cttctttttc
     9541 caatcctgtc ctcgaggatc tgcacatgga atattttata aaactttttt tgagtggtac
     9601 agcaaaaggc agcatctatg cccttatggc attaggctac accatggtat atggcattat
     9661 tcaactcatc aattttgccc atggcgagat ctacatgata ggtggtttca ccgcccttat
     9721 tctgggggga ttcttctttt ccaagggtat gcccgtcagc cttgtccttg ttttttcgat
     9781 tttgctttcg ataatgtatg ccgcggcatt cggttttaca atcgagaaga tagcctacaa
     9841 gccgctcaga gaaaagccac gcctgtcggc tttaataagt gccataggtg tttctataat
     9901 acttcaaaac tttgtccttc tctcccagac tgagaaattt ctaccatttc ccaattacct
     9961 tcctgaattc gcatggctat tgccatataa ggaataccta aattctaccc aactcataat
    10021 tctcataacg acagccataa taatggttct gctgactttg ctcataaaat tcactcgcat
    10081 gggaaaagcg atgcgcgcta ccgcacaaga tatgcacatg gcccaactcg tgggtgtcga
    10141 tataaatcaa gttatttcgc ttacttttgt catcggctcg gcacttgcgg ccattggggg
    10201 cgtgctcatc tgttcctaca tggggcaaat caattattac atcggcttca ttgctggcat
    10261 caaagctttc gttgccgcgg ttctaggggg tatcggaagt atccccggag ctgttctggg
    10321 aagtttcgtc ctgggttgga cggagagttt cggcacgggc tatgtttcca gcgattatga
    10381 agatgccttt gctttcctca ttctgataat catcctcctc ataaagcccg atggaattct
    10441 tggtcgaact cgacgacaga aggtctagat tatgaaagca agctcaattc ttaaagaaat
    10501 cggaaaatcc ctgattatag ccattggctg catggtcttg ttcttcccgc ttatggttat
    10561 gaaagtcaac gtgacaaggg gagtagctca tatcaatttt cgctggaatg ttcttcctct
    10621 cattggaatc gttgcattcg tgctctcttt tgtctggagc aaggctctcg agtggaacga
    10681 aaaacgggca agtgcaaaaa aggtagagaa tggactggac aacaaccgag cgctaaatgg
    10741 aagatatctt aagaacccag cccttcgtcc aatattcctt tttgcactct tcatatttgc
    10801 cgtggctttt cctttccttt ttggtatgta ccacacgaat gtcatgatca ctgcatttat
    10861 atatgtgatc ctaggcctag gcctcaatat catagttgga ctaggaggtc ttctaaatct
    10921 cggttatgca gcttttttcg gaattggagc ttatacttat ggccttatat ggaaatatat
    10981 tggaccttct ttcgttgcgg ctggcttcaa tccaggatgg ctcttttgga tatcgcttcc
    11041 tctggcaggc attgcggcaa cggtctttgg aataatcttg agtttgccag tattgagact
    11101 taggggcgat tatctagcaa ttgtcaccct cgcatttggc gaaattttcc acatggtaat
    11161 gcagaattct ggagatataa caggaggagc taccggtata agtcttattc ctcgtccatg
    11221 gtttttaggt cagaaactgc gccctccaga agctgccaca tatatatact tcatagctct
    11281 tgttttcgta atcataacga tattcgtcgt aagacgaatc gaagattcga gagtcggaag
    11341 agcgctcgaa gctatgcgcg aagatgaggt agcctgccag gcgatgggca tcgatatggt
    11401 caagaacaag ctgataacct ttgcggtcgg cacattctgg gcaggaattg ccggagtgtt
    11461 gatggctagc cagactactt atatcaatcc cgatagcttt actctatggg aatcgataat
    11521 gatccttatg gctgttgtca taggaggaac aggctctatt ccaggagtaa ttgccggagc
    11581 aatactcttg aaacttttgc ccgagtattt ccgcccattg gcgcaatacc gcatgctcat
    11641 atatggcatt gcgatgatcc ttgtcatcat tttcaagtcc gatggcctca ttcctcgcaa
    11701 gcgcaagcag tataccttca aagaaaagga gcttgcaaaa tgagcacaga gaaactgcag
    11761 taccatcatg aaatcctgga aatcgacaat ctagtcatga cttttggagg gctgcgcgca
    11821 atagatggag tatcgatgca tatcgacgaa ggagagatcg ccgctctaat aggcccgaat
    11881 ggcgccggaa aaacaacaat tttcaactgc atcacaggtg tacataagcc taccgaggga
    11941 aaggtaaaga tccgaaatcg agatggacgt gccgaggaga ttcagggact aaaacccaac
    12001 ctaatcaacc gtaaaggcct agcccgcacc tttcaaaata tccgcctttt caacaatatg
    12061 actgttctcg aaaatgtcat gattggccgc cacaattcgc tcaaggcagg ggttttcgca
    12121 gcaataatcc ggaatgctcc aacaaaggaa gaagagcaaa gagtcataga ggagagctat
    12181 acaatcctaa agaaactcaa actcgacgcc tatgtcaatg aaatggcaaa gaatctacct
    12241 tacggtgaac agcgacgcct cgagatagct cgcgcgcttg caaccgatcc ttttctgctt
    12301 ctcctggacg aacccgttgc tggaatgaat gctcaagaaa caaaagaact agaggagact
    12361 ataaatataa ttcgggacca ggaacacatc actatccttc tcatcgaaca cgatatgagc
    12421 cttgtcatgg atgttagcga gcgaatctat gtattggact atggtaaact catagccgaa
    12481 ggaagcccgc acgagatcaa gaagaatccc gatgtcatca aggcatatct aggagagtaa
    12541 aatggcgctt cttgaactta aagatgtccg taccttctat ggaaacattc aggccttgaa
    12601 aggcgtctcg atctccgtag aagaaggtga aatcgtaaca cttatagggg ccaatggagc
    12661 cggtaagaca accaccctca tgagtatctg cggtattacc ccaattcgct caggcgagat
    12721 tttcctcgat ggtcgcgata tttcgagaat gagtccccat aagatagtcc agatgggcgt
    12781 ttcacaagtt cccgaaggcc gccgcatttt tcctcagctt acggtctcgg agaacctcga
    12841 tatgggagcc tttctccgta acgataaaga gagcataaag caagacatgg aagaagtatt
    12901 cacaatcttt ccaaggcttg cggagcgtcg caatcagctc ggcggaacac tttctggggg
    12961 cgaacagcag atgctcgcta tctcgcgcgc cttgatggca agcccgcgcc ttcttttgct
    13021 agacgagcca tcgctagggc tggctccgct tattgtccag aacatattcg aggttatcca
    13081 aaagatcaat aaagaacgaa aaaccacgat tctactcgta gagcagaatg cgaatatggc
    13141 actcaagatc gcgaataaag ggtatgtcat gcagaatggc ctaatcaaga tcaccgatac
    13201 cgcgacaaac ctgctcaaaa atcaagaagt tcgcaaagca tatttggggc tttgacttgc
    13261 cagcattata ttttcagtat atttatggaa gcataatcac tgaagtataa tcataggata
    13321 aatatcgaag gaggcgagta tgaaagttgg tcagcgcatg acgcgaaatc ctgttacaat
    13381 tacgccggat acgactgttc ctgaagcgca agccattatg cggcgcgaaa agattagacg
    13441 gctaccagtg ctcgataaaa acggcaagct tgtcggtatt gtcagtgctc tcgacctcat
    13501 gcatgcatcg ccttcgcctg ccacaactct agatgtatgg gaaatgcatt atctgctatc
    13561 aaagcttact gtcgataaag taatgaccaa agatgtaata acgatcaccg aagatctccc
    13621 tatagaagaa gcagcccgca tcatggatga taatcagata tccggaattc cagttatgcg
    13681 cggtgaaatc ctggtcggca ttatcacgga atcggatata ttcaggcttt tcatcgagct
    13741 ttttggggcc agacataagg gcataaggct cacgcttctt ctacctgaga aaaggggaga
    13801 actggcaaag ctgtccaatg cgatcacgaa atgtggcggc aacatagtct cttttgctac
    13861 attcgaagga gaagatccaa ccaatgccta ctgcacagtc aaggttacgg gcgtcgaaaa
    13921 agaagccttg atccaagcga ttactcctgt cgttgaaaag gtagtggatg tgagggaaac
    13981 ataagacaca gcaccaagat agatcgatat agattagaat tagcgctggc gtctggagaa
    14041 agtccccttt tcttgtcgag atttctttgc ttctatagtt tcccgctgcc attccataag
    14101 agaactgcgc tcgaaatcga tgatgatgcc cagatggcgc ataaaatcat ctccaatctc
    14161 gagatgctct tttaagctct cgagcattgc tccctttctt cttatttctt cgaggatata
    14221 aaaaagctct gttactgtcc gagcgagtct atcgagagag gcgacaataa gcgtatcgcc
    14281 actgcaaagg ctatctagtg cacgcctcaa ttcaggcctg tctgcttcct ttgagagggg
    14341 tttttcctcg aatatttttt cagcgccagc cttttttaga gcctcgaatt gtgtatcgat
    14401 gtccgcctcg atgggtgaag gtctaatata gccttttctt tccgacattc tctcaactcc
    14461 ttaaaaccag cttacaaaaa acgcttagcc atcgatggat tcgatatcat ggcacgattt
    14521 cgtacactac aagcgcttct gagcgcgtcg catcgctcga gacgacagaa agacctcgtg
    14581 taatagccgc tgctgcgctc ccctgaagat cagaaaaatc cgcttccatg gttgtaaagg
    14641 gctttgtcga agccacggct ttaatatatt cggttccata gggaggacca agcaaatatt
    14701 gaaacttgtc gtttggtcct ggaaattgca ttgcctcgcc tgcgcgtatt ttgcccgaac
    14761 caccaaagcg gttcggccag atgagctgag ccacgccatt gacatctaca tggtaaattt
    14821 taaggtaggc atccttgttc gaagtcaggt agagcttgag ccgttcgcca tttcgataga
    14881 cggctcctac accccgatcc gtagataagc tgagctgaaa atctgacatt gcgtaggctt
    14941 gactacccaa agacggactg cctgatactt ggcttacaga acccggtaca agctggctca
    15001 aacttaccgc cgtctctaga gccttgatgg aaggctcaac agaaacatcg gctggcaaaa
    15061 ttcccttctt tacgagatat ctggtttcgc ttatcaacgc acccgtcgct agatccgtta
    15121 gggaaagagt ggtcagaact ccatctccat ctcgaatata ctttccatat agaatcgaat
    15181 ccgcacgatc ctgcccaaag aaaaccgcat attgctcgcg aattgccgga tccatagccg
    15241 ccgctacctg tttgtcgaaa agcttcatct ttgatgtgcg accgagcgca agccgaagtt
    15301 catcctcgaa ccatcgcgca aagggcgtag gaagctgcgt
//
LOCUS       DAOSOB010000050        14794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_4081_length_14794_cov_92.897619, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000050 DAOSOB010000000
VERSION     DAOSOB010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 14794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14794
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4081_length_14794_cov_92.897619"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..>2377
                     /locus_tag="PLS88_06330"
     CDS             174..>2377
                     /locus_tag="PLS88_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOU06993.1"
                     /translation="MDDTTGFDLTTIEGLLVDELAISQRIANELSIRLSQVQAVLSLS
                     AEGCTIPFISRYRKERTSNLDEVQVRDCIQKFQSYKNLEERRLEVLKGISSLGKLDAF
                     LYENITRATTLTELEDLWAPFKKKKKTRGMIAQEKGLGPLAELMSRASLEEIVGAARS
                     FIHRDEEHPELSVESAEDALSGARDILAERLSQDTSMRAYIKDVLLKNGELAIKGIGD
                     EKKRDQSTYQMYWDYREPLSTLKHHRVLAVNRGEREGELDVSIEVNEDLVEVRMLERM
                     RPANDQHKEAVIDGLFRLLLPAVRREIRSDLTESAESHAIEVFSTNLRNLLMQPPLRG
                     TRVLGIDPGIRTGTKCAALDETGKFLDYFVINQETKPEEGKKDIAAAVIKNRLSVIAL
                     GNGTGSHEVQRLIAEAIAENKLACRFAVVDEDGASVYSASDLAREEFPDLDLTIRGAI
                     SIGRRLQDPLAELVKIDPKSIGVGLYQHDVNQKQLANQLDEVVGSVVNQVGVNLNTAS
                     YSLLRYVSGINASLARKIVKFRDASGVIRNRSLLKEIPGLGEKTFEQCAGFFKIPESE
                     NELDNTWVHPENYALASEILPIVKFGREPKAEERAALKEKYEVGDITLNDIISELRKP
                     NRDPREDLPPPILQENVLNFEDLRPGMKVKGKVKNVVDFGAFVDIGIKESALIHVSEM
                     AERFVKNPMEVLKVGDVKDFTVISVDPVRRRIGLSLRANPKLETAQAELGAR"
     assembly_gap    2378..2477
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2674..3690
                     /locus_tag="PLS88_06335"
     CDS             2674..3690
                     /locus_tag="PLS88_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU06994.1"
                     /translation="MSILFEPFLGDTFLARGPTNLGLYIFRQSDADRAVLIDSGGDED
                     AGRRILRECERLGVELSTVINTHSNADHCGGNAFLSRRSNCSIASTEMEAVFLKYPQL
                     EPSFLSGSFPQKALRNKFLMAQPSQANCLLKPPCMLALDKEGKVQLSEGALASESLGA
                     PTSKDMSSSEDAFGLQIISLPGHYFSMVGVMTPDKVFFVADSLAGVPILEKYGIFFLY
                     DIEAELATLSMLESSEAEWFVPAHAEPTRDIKSLVDINRAKIFEIADVILAAVNVKEH
                     DGEGVSLEDVMAAVFSHYDIALNANQYVLVGSTIRSYLSWLCDQGRLEYGFEHHRMIF
                     RIKR"
     gene            3687..5567
                     /locus_tag="PLS88_06340"
     CDS             3687..5567
                     /locus_tag="PLS88_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06995.1"
                     /translation="MSQRNLGVNFAKIWMIMVCLFILGFFVHGGGPGAVYAQGHLAVE
                     LGHPVYAVIETAELRGVVTRLSSVKPYTSQQVAELLAIMMGRMEAFSPTEQELIRKYA
                     KEFKTGKGLEQSLWKDSSGVSQAGIRIETTTKLDAGSVYDLIDGSSSTALKDIWHFNS
                     RWVPYLKGDPLSWFSFKGEAGFTFDKIERDLYLPYEFTKEWDATHIWFGAPRNSDGTL
                     DYPTASYDIREDIAAETDDSSVMVRLSRFRRDWGIGSGSFSLSDTARPFVGLETVLRP
                     SKYFAISGLVGSLTNWQKSADEKSTAMKFYDENGDGKFDEGKDYYYYSAISYQKMLGL
                     QRMELFPFDWLTISATSTIVGAKRFELGYFSPLLFSVMYQNQLADIDNLAVQVDGQIL
                     VPRIGKFYASFYADEMEITNLSELFTKARNMFALQGGAKIPLPFWSFATLTAQYSKIE
                     PFVYSHYPTWYPDYRMQVDTSYTQDGENLGYYLPPNSDEFLVKLDMMPARDWRLSLKY
                     SFVRHGDNPSNKQGDLVIFGDVDKWLEYNWPDRTSKLDDYPDKNFLHDGIYDYNHIGN
                     ISVSWRPTVPPLLFGAEIPFELSAGYGISYTWYEDGTGAGRAIPASEWKNVFSMSMKL
                     FL"
     gene            complement(5585..6184)
                     /locus_tag="PLS88_06345"
     CDS             complement(5585..6184)
                     /locus_tag="PLS88_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU06996.1"
                     /translation="MEIPLRLVGRKDGSLFYGVPLYFRLIMAGMLILMISAIGVEGFN
                     TSVIGWIIIILLLLGFVYKEDWTFDPSSRHILSQIGFYPLLKKTGIAFEEVAYLQLAP
                     ISKGTIPGSKEEEAANREAFNEMRGKEAGEDSKAGIHSLFQRKKLYIALLIITKNEEH
                     YLIDMIPARRASRLSQTGKALAALVDCPFLDDLYSKSNL"
     gene            complement(6190..6471)
                     /locus_tag="PLS88_06350"
     CDS             complement(6190..6471)
                     /locus_tag="PLS88_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HOU06997.1"
                     /translation="MRTCITEMSKALEGADAALIEEFFYSLFTAAEADEMAKRWALVK
                     ELAKGTPQRKIAADLGLSLCKITRGSRELKKPGSAFRQLLDKLPSSRAT"
     gene            complement(6671..8068)
                     /locus_tag="PLS88_06355"
     CDS             complement(6671..8068)
                     /locus_tag="PLS88_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU06998.1"
                     /translation="MEGPRLKIKPFTELELSGKTVIFRPDINSPIDPKTKRIVNTNRI
                     EKTVPTLNALLEKGARVALIAHQGDTLDYQNLIPLAEHAEILSRLTNRRVSYIDDVCG
                     PAAQAAVKALSPGEAIILGNLRYLTEEISTFETVVKLTPQDMTKTWLVRSLAPLGDYY
                     VNDAFAAAHRNSPSMVAFQELLPSAGGFQLMDEYTALKSVKDNPRRPCVYVLGGAKIS
                     DAFDMMRKVLTDGSADYILTAGLTGIVMHIARGVDFGPVVQKFLVDHGLDVFIPEAKS
                     LLGEFGERYVLPVDLAYDENAGLSQSDARSLANAATFASASTLASASGVAKASALADQ
                     KSAPTTTPKRAEAPIEELPKDRLFPDIGHKTIELFKKYIDSAGSIFVNGPAGMYETEP
                     WADGTRELWRAIAEAPGYTVIGGGDTISAAAKFSELSKYGYVCTGGGAMVRFLAGKKL
                     PLIEAMEKAFDRDFS"
     gene            complement(8113..9150)
                     /locus_tag="PLS88_06360"
     CDS             complement(8113..9150)
                     /locus_tag="PLS88_06360"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HOU06999.1"
                     /translation="MAKVKVGVAGYGVIGQRLADGVALQQDMELVGVADVAVTLSVRA
                     LREKGMPYKFFLAAPEKREEFEKAGIPLSGSLEDLIQQVDVMLDATSAGVGAKNRVLY
                     EKYGKKGIFQGGEKNSVADVFFHGYANYEKGIGAQFLKLTSCNTTGLIRAVDCIDRKV
                     GLEKVAITIIRRVADPGDYHRGLTNALQIEHAPSHQALDLMTIMPHVDATGILVHTPV
                     THGHIITVVATPKKSVTPENVIEFFNEHPRIRVVSIEEGFLGNASLFRYARDLGNPRG
                     DMYEIAAWKDSVVMSGKDVMFAINIPQEAVVIPENMDAVRACMRMQLDRIEATDLTNK
                     YLGIGKWRNLG"
     gene            complement(9209..10096)
                     /gene="iolE"
                     /locus_tag="PLS88_06365"
     CDS             complement(9209..10096)
                     /gene="iolE"
                     /locus_tag="PLS88_06365"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HOU07000.1"
                     /translation="MEKDIRLAIAPIGWTNDDMPELGGEIPFEQCISEMALAGFEGSE
                     VGNKYPRDPEKLNKALKLRGLTICNAWFSSFLTTKPLAEVEKDFIKQCDFLYAVGARI
                     IGAAEQGNSIQGKPLPIFDAKPRFTDEEWKSLTEGLNHLGTIAKKKGMQLVYHHHMGT
                     GVQTTEEIDRLMEMTDPELVGLLYDTGHLVFSGEDHLAVLHKYLKRIKHVHLKNVRFE
                     VMEKAKKEKWSFLQAVRNGVFTVPGDGGLDFVPVFDALKSGGYKGWWVVEAEQDPAKA
                     NPLEYALIARRYIKEKAGI"
     gene            complement(10126..11991)
                     /gene="iolD"
                     /locus_tag="PLS88_06370"
     CDS             complement(10126..11991)
                     /gene="iolD"
                     /locus_tag="PLS88_06370"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HOU07001.1"
                     /translation="METVRLTVGQAIVKFLDNQFVEFDGKIEKFVGGVIGIFGHGVVV
                     GLGEALAAPGHGLTFIQAKNEQGAGHVAIGYAKQNNRRKIMAVTSSIGPGALNMVTAA
                     GTATVNHIPALFLPGDVFADRQPDPVLQQLEVPHDYTISANDAFKPVSRYFDRITRPE
                     QIMTALIHAMSVLTDPAQAGAVTIALPQDVQGEAYDYPVEFFEKRVHHIERRPPTMAQ
                     VSRIVDLIKAAQKPYIICGGGVRYSEAGRALADFCEKFGIPFGETQAGKGCIPWDHPC
                     NLSGIGVTGSLAANRLAKEADLIIGAGTRFGDFTTCSKWLFQNPSAKFVSINVSPFDA
                     YKMNAEPIIADAKLTLQAVGRKLGTAAYRAQWGEAIAKAREYWKREVDALYNQEDPKG
                     LSQIRVIGELNDVLLPKNAIVVSGSGSIPSDMQRTWRTRVPGTYHMEYGFSCMGYEIA
                     ATLGCKFAEPERECVAIVGDGAYTMLHTELLTAVQEGKKIIVVVCDNSGFQCIDNLQH
                     NQGIAHFANEWRLRDPETGLLVGDQLPVDFAKNAESWGALGLRASNVEEFRKAMRRAL
                     RAKGPVVIHTRVSPKSMTQGYESWWRVGTAEVSSNPEVAKAAQEMKEELTKARKF"
     gene            complement(12077..12961)
                     /locus_tag="PLS88_06375"
     CDS             complement(12077..12961)
                     /locus_tag="PLS88_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HOU07002.1"
                     /translation="MVLRHSDDYVEGYELLVLRKSERADMLMDFASLSLKKGESWHSS
                     SKDERAFLLAEGSAEIIVGKKVDWIGLGIEGFAKNDSVTIDLLPGGETKIRASRTSLL
                     DENPTVLHLPKETKIAIKALSERALFYVSSTDNDMDFSPRLYLPYECRVEYRNEGTMQ
                     GTATRIVRTVFDKQNAPWSNLVLGEDVNLPGRWSSYPPHHHPQPEIYHYRFFPAQGYG
                     MTALGTEAYQLHNRDTLLITGNKDHPQVAAPGYAMWYLWVIRHLDGNPYISPEFTPEH
                     MWVDQPGAQIWEMKKPSH"
     gene            complement(12969..13988)
                     /gene="iolG"
                     /locus_tag="PLS88_06380"
     CDS             complement(12969..13988)
                     /gene="iolG"
                     /locus_tag="PLS88_06380"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HOU07003.1"
                     /translation="MTDKITVGVIGAGRIGKIHVSNIVYYMPEARVKTIADANLTPEI
                     EAWAKNLGIPHVTNDAEDIFEDPEISVVLICSSTDTHADYIIKAARAGKNIFCEKPVD
                     LSVEKVKKALAAVKEAGVRLQIGFNRRFDHNFAHVRKLIDDGAIGQVQLVKITSRDPA
                     PPPISYVKVSGGIFLDMTIHDFDMARFQAKSEVEEVYAQGAVLIDPEIGKAGDVDTAI
                     IMLKFANGALGVIDNSRKAVYGYDQRVEVFGSAGAAAAENDTPSQVRLSDTSGVHADK
                     PLYFFLERYKDAYIEEMKAFFKAIKEGTETPVTGQDGLMDLAIGLAAKKSLAEHRPVK
                     IFEVL"
     gene            complement(13985..>14794)
                     /locus_tag="PLS88_06385"
     CDS             complement(13985..>14794)
                     /locus_tag="PLS88_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOU07004.1"
                     /translation="ALGLSEFFDLAASLGMEAVELRNDIRDSSVTDGIEGPKVLRMAK
                     AAGTNGVKIITINALQQFNLPSARMKALGELESLLTLCRELECPALVLCPNNRPDDQR
                     TAKQKYHDTIEALNTYAPLFSTAGILGYIEPLGFEISSLSSAEVALKAIGESCASCYR
                     VLIDTFHSYLGPDKPEFFGDPSVIEKIGLVHVSGVEASIEKSAFTDAHRVLPGPKDIM
                     KSAELVHKLELAGYKGFYSFEPFSPEIQSLEKNELAGIVRNSIQYIQEVCL"
BASE COUNT         3599 a         3514 c         3640 g         3941 t
ORIGIN      
        1 tggtatattc agctcaatgt aacgaatgct gttcaaagga tataccatat atatctaaaa
       61 atccagcgaa ttatcattct atgatagaac ttcgcagaga tttgtagcga actcgtcatt
      121 ccatcatttc taccttgaaa ttttcttgaa cttcccgtaa cgttagcttc gctatggacg
      181 acacaacagg ttttgatcta accacaatag aaggtctttt agtcgatgag cttgctattt
      241 cacagagaat agcgaatgag cttagtattc ggctttccca ggtgcaggcg gttttaagtc
      301 tttcagccga aggctgcacg attccattca taagtcggta tcgcaaggag cgaactagca
      361 atctcgatga ggtacaggtt cgcgattgta tccaaaagtt ccaaagctac aaaaatctcg
      421 aagagcgcag gctcgaggta ctcaaaggta tctcatctct gggtaaactc gatgcttttt
      481 tatatgagaa tattactcgc gctactactc tgaccgaact cgaggatctc tgggcaccat
      541 tcaagaaaaa gaaaaagaca cgtggcatga tcgctcagga gaagggacta ggccctcttg
      601 ctgagctgat gtcaagagct tccctcgagg agatagttgg tgccgctcgt tcttttatcc
      661 atagagatga agaacaccct gagctttcag ttgaaagcgc cgaggatgcg ctttcgggag
      721 cgcgggatat ccttgcagaa cggttatcgc aggatacaag catgcgggct tacataaagg
      781 atgtcttgct aaagaatgga gagcttgcga ttaaaggcat aggcgacgag aaaaagcgag
      841 atcagtctac ttaccagatg tattgggact atcgagagcc actctcgact cttaagcatc
      901 acagagtgct tgcggttaat cgcggtgaga gagaaggtga gctcgatgta tctatcgaag
      961 tgaacgaaga tcttgtcgaa gttcgaatgc tagagcgcat gcgaccagcc aatgatcagc
     1021 ataaggaggc agttatcgat gggcttttcc gccttctctt accggcggtg cgaagagaga
     1081 tccgttccga tctcacagaa agcgcggaaa gccacgcaat cgaggtgttc agcacaaatt
     1141 tgcgcaatct tcttatgcag ccgcctctgc gcggtacaag ggtcttaggc attgatccgg
     1201 gaatccgaac cggtacaaag tgtgctgcgc tcgacgaaac tggtaaattc ctggattact
     1261 ttgtcataaa ccaggagacc aagcccgaag agggcaagaa ggatatcgct gcagcggtaa
     1321 taaaaaacag gctttctgtt atagcattgg gaaatggcac cggaagccat gaggttcaga
     1381 ggctgatagc ggaagcaata gcagaaaaca agcttgcctg ccgctttgct gttgtcgacg
     1441 aggatggagc gtctgtctat tcggcaagcg atcttgcgcg agaagagttt ccggatctag
     1501 acttgacgat ccgcggggcg atctcgatag gacggcgtct gcaagatccg cttgcggagc
     1561 tcgtcaaaat cgatcctaag agtatcggcg tagggctcta tcagcacgat gtgaaccaaa
     1621 aacagctagc aaaccagcta gatgaggttg ttggatcggt tgtgaaccag gttggtgtca
     1681 atctgaatac cgcaagttat tcgttattgc gatatgtgtc gggaatcaat gcgagcctag
     1741 caaggaaaat cgtcaagttc cgagatgcga gcggtgtgat tcgaaatcgt tctctgctaa
     1801 aagaaattcc ggggcttggt gagaagacat ttgagcagtg cgcaggtttt ttcaagatac
     1861 cagaaagcga aaacgagctc gataacacct gggttcaccc tgaaaactac gcacttgcct
     1921 ctgagatttt gcccatagtt aagtttggca gagaaccaaa agcagaagaa agagcggctt
     1981 tgaaagagaa gtatgaggtt ggcgatatta ctttgaatga catcatttcc gagcttagaa
     2041 aaccaaatcg cgatccacgc gaagaccttc cgccacctat tttgcaggaa aatgtgctga
     2101 atttcgaaga tcttcggcca ggaatgaaag taaaaggaaa agtgaagaat gttgtcgatt
     2161 ttggtgcctt tgtcgatatc ggaataaaag aaagcgctct tattcatgtc tcggagatgg
     2221 cggagcgctt cgtgaagaac cccatggagg tgctaaaagt tggcgatgtc aaggacttta
     2281 cagttatcag cgtcgatccg gtgcggcggc gcataggctt atcgcttagg gcgaatccta
     2341 agctggaaac agcacaagct gagcttgggg ctcgcaannn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnntgt tcggtgtcgg tttttgtgcc gctaatcccc ccaaccccag
     2521 cggccaaccc tacccccgat gccgacattg tacgaaggct ttttttgtgc caggctggcg
     2581 agttttagcc catatttcat ttgatttatg gaaacattat agaaacacca tagaaagctt
     2641 gccttcaggc cataaacaga gtaacaatac tctatgtcca ttttattcga acccttctta
     2701 ggcgatacct ttctagcccg cgggccgacc aatctcggac tctatatttt cagacaaagc
     2761 gatgctgatc gcgccgttct catcgattca ggtggcgacg aagatgcagg aaggcgaatt
     2821 cttcgagaat gcgagcggct gggtgtggag ctctctacgg tcataaacac tcattccaat
     2881 gccgatcatt gtgggggaaa tgctttttta tcgagacgaa gcaattgttc catagcttcg
     2941 acagaaatgg aagcggtttt tttgaaatac cctcagcttg agccttcgtt tttgtctggc
     3001 agttttccgc agaaggcgtt gaggaacaag tttcttatgg cacagccttc gcaggcgaat
     3061 tgcttgctca agcctccatg tatgctcgca ctcgataaag aggggaaggt gcagctatcc
     3121 gaaggcgcac ttgcaagtga atcgctgggc gcacctacaa gtaaagatat gagctcatca
     3181 gaggacgctt ttggcttgca gattatctct ctaccgggcc attatttcag catggttggc
     3241 gttatgactc cagacaaggt gtttttcgtt gcggattctc ttgccggagt gccaattttg
     3301 gaaaaatatg gcattttttt cttgtacgat atagaagcag agcttgcgac actttcgatg
     3361 ctcgagtcta gcgaggcaga atggtttgta cccgctcatg ccgagccgac tagagacata
     3421 aaatcgctgg tagatataaa tagagctaag atcttcgaga ttgcggatgt catcttggca
     3481 gcggtgaatg tcaaagaaca tgatggagaa ggggtttcgc tcgaagatgt tatggccgcc
     3541 gtattctctc actatgatat agctctaaat gccaatcagt atgtgctagt tggctctacg
     3601 attcgttcat acctttcttg gttgtgcgat caaggtcggc tcgaatatgg tttcgagcat
     3661 catagaatga tttttaggat aaagcgatga gccaaagaaa tctcggggtt aattttgcca
     3721 aaatatggat gataatggtc tgcttgttca ttctaggttt ttttgtacat ggcggtggac
     3781 ctggggctgt gtatgcgcag ggacatttgg ctgttgagct tgggcaccct gtctatgcgg
     3841 tgatcgaaac cgcggaactg cgcggagttg ttacaagact ctcttctgta aagccttata
     3901 ctagccagca ggttgctgaa ttacttgcaa ttatgatggg gcgcatggaa gccttttcgc
     3961 cgacagaaca agagctgatt cgcaaatacg ccaaggaatt caaaactgga aaaggcttag
     4021 agcaatctct atggaaggat tcatccggag tctctcaggc ggggattcga atcgagacca
     4081 cgacgaagct cgatgcaggt agcgtctatg atctcatcga tggttcttct tctacagctc
     4141 tcaaggatat ttggcatttc aattctcgct gggtaccata tcttaagggc gatccgctgt
     4201 catggttttc tttcaaaggg gaagcgggct ttacatttga caagatcgag cgggatctat
     4261 atttgcctta tgaatttaca aaggagtggg atgccactca catttggttt ggagctcctc
     4321 gcaattcaga tggaaccctc gattatccta ctgcgagcta cgacatccgc gaggacatag
     4381 cggcagaaac agatgatagt tcggttatgg tgcgcctctc gagattcagg cgggattggg
     4441 ggataggttc gggctctttc tccctttcgg atacggcgcg gccttttgta ggccttgaaa
     4501 cagtgctgag gccttcgaaa tatttcgcca tttcaggact tgtgggttct cttacgaatt
     4561 ggcagaaaag cgcggatgag aaaagtactg caatgaagtt ttacgatgaa aatggagatg
     4621 gcaaatttga tgagggaaaa gattattact actactctgc gatctcctac cagaaaatgc
     4681 tcggccttca gcgcatggag cttttcccct tcgactggct cactatctcc gcgacgagta
     4741 ctattgtcgg agccaaacgc ttcgagctgg gctatttttc gccactgctt ttttcggtga
     4801 tgtatcagaa ccagctagcg gacatcgata atctagctgt ccaggtagat gggcagatcc
     4861 ttgtgccgag aataggtaaa ttctacgcat ccttctatgc cgacgagatg gaaataacga
     4921 atttgtcgga gctctttacc aaggcgcgga atatgttcgc cttgcagggc ggcgcaaaga
     4981 tacctcttcc gttttggtct tttgcaactc tgactgcgca gtacagcaag atcgagccct
     5041 ttgtgtattc acattatcct acctggtacc ctgattaccg aatgcaggta gatacttcgt
     5101 atactcaaga tggagaaaat ctgggttatt atctaccgcc caactcagac gaattcttag
     5161 tcaagctcga tatgatgcca gctcgcgatt ggcgactttc tctcaaatac agttttgtac
     5221 ggcatggaga caacccaagc aataaacaag gagacttagt aatatttggt gatgtagata
     5281 aatggcttga atataattgg ccagatagaa cttccaaatt ggatgattac ccggataaaa
     5341 attttctcca cgatggcatc tacgactaca accacattgg aaacatcagt gtttcatgga
     5401 gaccgacagt tcctccgctg ttgtttggtg cggagattcc ttttgagcta agcgcaggtt
     5461 atggtatttc atatacctgg tacgaggatg ggacaggagc cggcagagcc attcccgcat
     5521 ctgaatggaa gaatgtgttc tcgatgtcta tgaagctgtt cttataatat aaagcgttgt
     5581 gcgctcaaag gttcgattta ctatacaaat catcaagaaa ggggcagtcc acaagcgccg
     5641 cgagtgcttt tcctgtttga gaaagacgtg aagctcgacg ggcaggaatc atgtctataa
     5701 ggtagtgttc ttcgttcttg gtgataataa gaagcgcaat atagagcttt ttcctttgga
     5761 aaagagagtg aattccagct ttgctgtcct ctccagcctc tttgccgcgc atctcgttaa
     5821 aagcctctcg gtttgcagct tcttcttctt tacttccagg gatggtgcct tttgaaatag
     5881 gagctagctg gagataggcc acttcttcga aggcaatgcc ggtctttttg agcaggggat
     5941 agaatccaat ctgacttaaa atatgccttg aggaaggatc gaatgtccaa tcttctttgt
     6001 aaacaaagcc aagcaatagg agaataatga ttatccagcc tatgacactt gtgttgaacc
     6061 cctcgactcc gatggcactt atcataagta tcaacattcc agccattatg agacgaaaat
     6121 atagagggac cccatagaaa agcgagccat ctttgcggcc gacaaggcga agaggtattt
     6181 ccatattatc tatgttgccc gagatgaagg caatttgtcc agcagttgac gaaaagcaga
     6241 accaggtttc tttagctccc tggagccgcg agttattttg catagagata atcctaggtc
     6301 ggcggctatt ttgcgttgtg gcgtgccttt tgccaactct tttacaagag cccagcgctt
     6361 agccatttca tcggcttcag cagcggtaaa aagcgagtag aagaattctt cgataagggc
     6421 ggcatctgca ccttcgaggg ccttggacat ctctgttata caagtgcgca ttagttcggg
     6481 atttcttggc atatttatta attatactat gaaaacccga aagggtcaaa aatgccctac
     6541 gtttgcacct ctgcttttgc acaaaattgt cgtgcaccca gcggctaagg tcgcggcgtt
     6601 tcgctctttt ccgcgcacag cccgtcgtat ctcgccattg gtatcgtgat gcctttttcg
     6661 gtcaatagca ttagctaaaa tctctatcga acgctttctc catagcctcg attaggggta
     6721 gctttttgcc agcaaggaag cgcaccatcg ctccgccgcc cgtacataca tagccgtatt
     6781 tcgacaattc ggagaacttg gcagccgcgc tgattgtgtc gccgcctcct attacagtat
     6841 aaccgggagc ctcggcaatt gctctccata gttctcgggt gccatcagcc catggttcag
     6901 tttcatacat acccgctggg ccattgacga aaatcgagcc agcagagtcg atatacttct
     6961 tgaagagctc gatggtcttg tgcccaatgt ccgggaaaag cctatctttg ggtagctctt
     7021 cgatgggagc ctctgctcgc tttggagtcg ttgtaggagc gcttttttgg tccgctagag
     7081 ccgatgcttt cgcaacgccc gatgcgctcg ctaaagtcga tgcgcttgca aaagtcgctg
     7141 cgtttgcaag ggaccttgca tcgctttgtg ataagccagc attctcatca taggcgaggt
     7201 caacagggag cacatagcgc tcgccaaact cgccgagcaa agattttgct tctggaatga
     7261 agacatcgag cccgtgatct actaagaatt tttgaactac agggccgaaa tcgactccgc
     7321 gcgcgatgtg catcactatg ccggtgagac ccgccgtgag aatatagtct gcacttccgt
     7381 ccgtcaaaac cttacgcatc atatcgaatg cgtcagatat ctttgcacca ccgagcacgt
     7441 agacacaggg gcggcgagga ttgtctttaa cggatttgag cgccgtatac tcatccatga
     7501 gctggaatcc ccctgcactc ggcaggagtt cctggaaagc gaccatagag ggcgaattgc
     7561 ggtgcgctgc agcgaatgca tcgttcacat agtagtcgcc gagcggagca aggctgcgta
     7621 cgagccaggt tttggtcata tcctgtggag tgagcttgac cacggtttcg aaggtactta
     7681 tttcctcggt gagatagcgc aagtttccga ggattatcgc ctcgcctggc gatagcgctt
     7741 ttactgctgc ctgcgctgcc ggcccacaga catcgtcgat atatgaaaca cgtctattcg
     7801 tgagccttga aagtatctcg gcatgctcgg ctagaggaat gaggttctga taatctagcg
     7861 tgtcgccttg gtgtgcaatt agggcaaccc tggccccctt ttctagcagg gcattcaggg
     7921 tcggtactgt cttttctatt cgatttgtgt tgacgatacg ctttgtcttc ggatctatcg
     7981 gagaattgat gtctggtctg aagatcacag tctttccact gagttctagt tcagtgaatg
     8041 gttttatctt gagtcgtggt ccttccatat agagcttctc ctttatttta ttttagccct
     8101 tataggtgta ggtcagccca gatttcgcca cttgcctatg ccaagatatt tattggtaag
     8161 gtctgtagct tctatgcggt caagctgcat gcgcatgcaa gcgcgaacag cgtccatgtt
     8221 ctctgggatt acgactgctt cctgaggaat atttatagcg aacataacat cttttccgct
     8281 catgacaacc gaatctttcc aggcggctat ctcgtacatg tcgccgcgag gatttccaag
     8341 atcgcgagca tagcggaaga ggctcgcatt gccaaggaag ccttcttcga tagatacaac
     8401 gcggattcgt gggtgttcgt tgaagaactc aattacattc tcgggtgtga cagatttctt
     8461 gggcgttgcg acaacggtga tgatgtggcc gtgcgtaaca ggagtatgta caaggatgcc
     8521 cgttgcatcg acatgtggca ttatggtcat aaggtcgaga gcttggtggc ttggggcgtg
     8581 ctcgatttgc agagcattcg tgaggccgcg gtggtaatcg cctggatcgg ctacacggcg
     8641 gataatggta atggcgactt tttcgaggcc gaccttgcga tctatgcagt cgacagcgcg
     8701 gattaggcct gtagtattgc agctcgtcaa tttgagaaat tgggcgccga tacctttttc
     8761 gtagtttgcg tagccatgga agaacacatc ggcaaccgag ttcttctcgc cgccctggaa
     8821 aatacctttc ttgccatatt tttcgtagag cacacggttc ttggctccga caccagcgct
     8881 tgtcgcgtcg agcatgacat cgacctgctg aattagatct tcgaggcttc ctgaaagtgg
     8941 aatacctgcc ttttcgaact cctcgcgttt ttcgggagcc gcaaggaaaa acttataggg
     9001 catgcctttt tcgcgcagtg ctcgcacaga aagcgtcacc gcaacatcgg cgacaccaac
     9061 gagctccatg tcttgctgca atgcaacgcc atctgcaagg cgttgaccaa ttacaccata
     9121 tcctgcgaca ccaactttga cttttgccat gatatcctcc caaaaatgcg cattcaaatg
     9181 cgtataaaac cagacattga gatgtttatc aaatacccgc cttttctttt atatacctgc
     9241 gggcgatcag tgcatactcg agcggattgg cctttgcggg gtcttgttcg gcctcgacga
     9301 cccaccagcc tttatagcct ccgctcttta gtgcatcgaa taccggtaca aagtctaaac
     9361 ctccgtcccc cggtaccgta aagactccat tgcggacagc ctgcaagaag ctccactttt
     9421 cctttttcgc tttttccatc acctcgaatc ggacattctt tagatgtaca tgcttgatgc
     9481 gctttaagta tttatgcagc actgcgagat gatcttcgcc tgagaacacg aggtgccccg
     9541 tatcgtacaa aagtccgaca agctctggat cggtcatctc cattaggcgg tctatttcct
     9601 cggtggtctg cacgccggtt cccatgtgat ggtgatatac aagctgcatg ccttttttct
     9661 ttgcaatcgt accgaggtga ttgagccctt ctgtaaggct cttccattct tcatctgtga
     9721 atctgggctt tgcatcgaat atagggagcg gtttgccttg aatggaattg ccttgttctg
     9781 cggcaccgat tatacgcgct cccactgcat acagaaaatc gcactgtttt ataaaatctt
     9841 tctcgacttc cgcaaggggt tttgtcgtca gaaacgaaga aaaccacgca ttgcagatcg
     9901 ttagtcctcg gagtttgaga gctttgttca gcttctcagg gtcgcgcgga tatttattgc
     9961 cgacttcgct gccttcgaat cccgcgagcg ccatctctga aatacactgc tcgaagggaa
    10021 tttcaccgcc tagttccggc atatcgtcgt tggtccaacc tattggggca atggccaatc
    10081 gtatgtcctt ttccataatc gctcctcttg aaagctcttt aaggcttaga attttcgtgc
    10141 cttggtcagc tcttctttca tctcttgagc tgcttttgca acttctggat tgctcgaaac
    10201 ctcggcagtg cccacgcgcc accaggactc gtatccttgc gtcatagatt tgggcgatac
    10261 acgggtgtga ataactaccg gtcctttggc gcgaagtgca cggcgcatag cctttctgaa
    10321 ttcttcgaca ttggaagccc taagaccaag cgcaccccag ctttctgcgt tcttcgcaaa
    10381 atcgacaggt agctgatcgc caacaagaag accagtctct ggatcgcgca ggcgccattc
    10441 gttggcaaaa tgcgctatgc cctggttatg ctgaagatta tcgatgcatt ggaaaccgga
    10501 gttgtcgcag acaaccacga tgattttttt cccttcctgc acggcggtca gtagctcagt
    10561 gtggagcatt gtataggcgc catcgcccac aatggcgaca cattccctct cgggttctgc
    10621 gaatttgcag cctagagttg cggctatctc ataacccatg cacgagaatc cgtactccat
    10681 gtgataggtg ccgggtacgc gagtgcgcca tgtacgctgc atgtcgctag gaatcgagcc
    10741 ggagcccgaa acaacgatcg cattcttggg aagaagaaca tcgtttagct ctccgatgac
    10801 acggatttga ctcaagccct ttgggtcttc ctgattgtag agagcatcga cttctcgctt
    10861 ccaatattct cgggctttgg cgattgcctc gccccattgt gctcgatagg ccgcagtgcc
    10921 aagcttgcga ccaacagcct gcaatgtgag ctttgcatct gctatgatcg gctcggcatt
    10981 catcttgtag gcgtcgaaag gcgaaacatt gatagaaacg aattttgcag aaggattctg
    11041 aaagagccac ttcgaacaag tggtaaaatc tccaaatctc gttcccgcgc caattatgag
    11101 gtctgcttct ttggccagcc tattggcggc aaggcttccc gtaacgccga ttccggatag
    11161 gttgcaggga tgatcccatg gaatgcaacc tttgcctgct tgcgtctcgc cgaaaggtat
    11221 tccaaacttt tcgcagaaat cggcaagagc tcgaccagcc tcggaatagc gcactccgcc
    11281 gccgcagata atatagggct tctgtgcagc ctttattaaa tcgacgatgc gggaaacttg
    11341 agccatagtt ggtggacgac gctcgatgtg atggactcgc ttttcgaaga attccacagg
    11401 gtagtcgtat gcctcgcctt gtacatcctg aggaagcgct atagtcaccg cgccggcctg
    11461 agctggatcg gtcaaaaccg acatcgcatg aataagtgct gtcatgattt gctccggcct
    11521 tgtaatgcgg tcgaagtatc tgctgactgg cttgaaagcg tcgtttgcgc ttatcgtata
    11581 atcgtgcgga acttctagct gttgaagtac tggatcgggc tgacggtcgg caaagacatc
    11641 tccaggaaga aagagcgccg gaatgtgatt tactgttgcc gtgccggctg cggtgaccat
    11701 atttagggcg ccagggccaa tagagcttgt tacagccatg atttttctgc ggttgttctg
    11761 ttttgcatag cctatagcaa catgacccgc accttgttca ttctttgctt gaataaaggt
    11821 gagcccatgg ccgggcgcag caagagcttc gccaagtcca actaccacgc catggccaaa
    11881 aataccaatt acgccaccca caaatttctc gatttttccg tcgaattcca caaactgatt
    11941 atcgagaaat ttcacaatgg cttggccgac ggtcaatcga actgtttcca taaataacct
    12001 cttttcaatg tgctcgttgg gacattctta tcggcatctc aaaagagcgt cagattattt
    12061 tgcctggccg ctcagatcaa tgagaaggct tcttcatctc ccatatttgc gctcctggct
    12121 ggtctaccca catatgttca ggcgtaaatt cggggcttat ataggggttt ccgtcgagat
    12181 gacgaataac ccatagatac cacatggcat agccgggagc cgccacctgg ggatggtcct
    12241 tattgccggt gataaggaga gtgtctctgt tgtgtagctg atacgcctct gtccccagag
    12301 ccgtcatacc atagccctgc gccggaaaga atctgtaatg atagatttcc ggctgaggat
    12361 gatggtgtgg aggatagctc gaccagcggc cgggaagatt gacatcttct ccaagcacaa
    12421 ggttcgacca tggtgcgttt tgcttatcga agaccgtgcg aacaattcgt gttgcggttc
    12481 cttgcattgt gccctcgttg cggtattcga cccgacattc gtaaggaaga tataatcgcg
    12541 gagaaaaatc catatcgtta tcggtggagc tgacatagaa gagggctctc tctgaaagcg
    12601 cctttattgc aattttggtc tctttaggaa ggtggagcac agtaggattt tcatccaata
    12661 gacttgtgcg agaggctcgt atcttagttt cgcctccggg gagcaaatcg atagttaccg
    12721 aatcgttctt ggcaaatcct tcaataccaa gaccaatcca gtcgaccttt ttcccaacta
    12781 ttatctcggc gctgccttca gccaaaagaa aagcgcgctc gtccttagaa ctcgagtgcc
    12841 aggattcacc cttcttcaag ctcaaagaag cgaaatccat tagcatatcg gccctctcgc
    12901 tctttctaag tacgagaagc tcataccctt ccacataatc atctgaatga cgcaatacca
    12961 tcgtttcctc acaatacttc gaatattttg acaggcctgt gctcggctaa agattttttt
    13021 gccgcaagcc ctatagcaag gtccataaga ccgtcttgtc ccgtaactgg tgtttcggtg
    13081 ccttctttga tcgctttgaa aaatgccttc atctcttcaa tataagcatc tttgtaacgc
    13141 tctaggaaga aatagagcgg cttgtcagca tgtacgccag acgtatcgct aaggcgaact
    13201 tgggaaggcg tatcgttttc tgccgcagcg gctccggcag atccgaatac ctcgacacgt
    13261 tggtcgtatc cgtatactgc tttgcgcgag ttatcgatta cacccagcgc tccatttgcg
    13321 aatttaagca taatgatggc ggtgtccaca tcaccagcct tgccgatttc cggatcgata
    13381 agcaccgctc cctgagcata gacttcttcg acttcgcttt tcgcctggaa gcgcgccata
    13441 tcgaaatcgt gtatagtcat atcgaggaag ataccgccag aaaccttgac ataagatata
    13501 ggcggaggag caggatcgcg ggatgtaatc tttacaagct gaacctggcc aattgctcca
    13561 tcatctataa gcttgcgaac atgcgcaaaa ttgtggtcga atcgacgatt gaagcctatt
    13621 tgaaggcgca cgccagcttc cttgaccgca gcaagcgctt tcttgacctt ctcgacactc
    13681 agatcgactg gtttttcgca aaagatgttc ttgcctgctc tagctgcctt gattatgtaa
    13741 tcggcatggg tgtctgtcga agaacaaatt agaactaccg atatttccgg atcctcgaag
    13801 atatcttcgg catcgttagt gacatgcggt atgccaagat tttttgccca tgcttcaatt
    13861 tccggcgtaa gattcgcatc ggcgattgtc tttactcggg cttctggcat gtaatataca
    13921 atgttcgaaa catggatttt cccaattcgc ccagcgccga tgacgccgac ggtgattttg
    13981 tctgtcataa gcaaacctcc tgtatatatt gaatgctgtt gcgcacgatg ccggcaagct
    14041 catttttctc taacgactgg atttcaggtg agaaaggttc gaaggaataa aagcctttat
    14101 agccagccag ctctaattta tggaccagtt ctgcgctttt cataatatcc ttgggtccgg
    14161 gaagcacacg atgtgcatcc gtaaatgccg atttttcgat ggaagcttct acgccggata
    14221 catgcactag gccgattttc tctatgactg aaggatcacc aaagaattct ggcttgtcgg
    14281 ggcctaggta gctatggaat gtatcgatga gaacacgata gcacgaagcg caactttcgc
    14341 cgatagcctt gagcgccact tctgccgaag atagcgaaga gatctcgaaa cctaggggct
    14401 cgatataacc aagaattcca gctgttgaaa agagtggcgc ataagtattc agcgcttcga
    14461 tcgtatcgtg atatttttgt ttcgctgtcc tttggtcatc cgggcgattg tttgggcaaa
    14521 gcacaagtgc cgggcattcc aattcccgac agagcgtcag cagagattcg agctcgccga
    14581 gcgccttcat tcttgcactt ggcagattga attgctgaag cgcattgatc gttatgatct
    14641 taacgccatt ggtaccagcc gccttggcca ttcgcaaaac tttcggccct tctattccat
    14701 ccgtaaccga actgtcccga atatcgttcc tcaattcgac agcttccatg ccaagacttg
    14761 ccgccaaatc aaagaactcc gaaagcccta gcgc
//
LOCUS       DAOSOB010000051        14323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_4259_length_14323_cov_68.967620, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000051 DAOSOB010000000
VERSION     DAOSOB010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 14323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14323
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4259_length_14323_cov_68.967620"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1450
                     /locus_tag="PLS88_06390"
     CDS             29..1450
                     /locus_tag="PLS88_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU07005.1"
                     /translation="MNNESSNELYSKRYSILTAVMLGGIMGPIDASIINVSLPSIAQA
                     FDVSIPIAQWVPMIYLLAISSLLLFYGRLGDMLGYRKVYLSGLVGFLIASGLCAFSSS
                     IYWLIAFRAIQGLAAGMMMAVPYAIITTSFPPEERGKALGINAISISAGLAIGPSLGG
                     FITALLGWRFVFLINIPIGIIGLLWGRHSIPESKGQPGRIDVLGALLAFISLFSILFL
                     INRSHSEGLAWLSPFLLAIAILAGIGFIWTEKRTNQPMLHLALFRNVTFCFANLSALL
                     NFMSQYVMVFLTPFYLQRVVHCAPNKLGLIMTSFPLAVMAVAPFSGSLSDRIGTRVLA
                     CSGASICAISLFFMAGLSSSANSKDVIWRMVLFGIGTGIFQSPNNSTVMGNVPREHLG
                     VASGILATMRNIGMALGIAIGGAVLYAFAPPSLLQKSFLEGWEAEVFLSALKYAYSFG
                     GALSVAAAFASLVRSRAASSAAL"
     gene            complement(1366..1920)
                     /gene="tpx"
                     /locus_tag="PLS88_06395"
     CDS             complement(1366..1920)
                     /gene="tpx"
                     /locus_tag="PLS88_06395"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOU07006.1"
                     /translation="MANITFKGNPIQTIGSLPAKRTTAPDFRLTKSDLSDVGLADFEG
                     KIKILNIVPSLDTGVCAASARAFNKAASSLDDVVILTISRDLPFAQKRFCEAEGIDKV
                     VTLSELRTRDFGKAYGVEIISGPMAGLLSRAVVVLDKANKVVYTEQVPEIAQEPNYKA
                     ALEAARLLTKEANAAATDKAPPKL"
     gene            complement(2002..2742)
                     /locus_tag="PLS88_06400"
     CDS             complement(2002..2742)
                     /locus_tag="PLS88_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU07007.1"
                     /translation="MTNMLFSIVLAFSLSAMTGYAQGWPESKESDFFDAVKGKLEIVF
                     LGHGSLLLVYNGSNIYIDPVSQYANFSKYPKADLILITHEHGDHLDPKAIAALSKPET
                     HIILPDASRKKLGNGEALQHGHSIDVAGINVLAVPAYNITSSRLGYHPKERKDNGYVL
                     TIGSLHIYIAGDTEPIPEMANLGKIDIAFLPMNLPYTMTPAQVAQAARTIKPKVLYPY
                     HFGSTDTSEITRLLASDKEIEVRIRNLE"
     gene            2937..4103
                     /locus_tag="PLS88_06405"
     CDS             2937..4103
                     /locus_tag="PLS88_06405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU07008.1"
                     /translation="MKKVTSGLFNDGYLPIIDGVTVTVRNYAYWLRKKLGPTYVVAPF
                     VPNYKDNDPFPVIRFLSIPTIVRPPYRIGLPELDMRLPSILKKREFDIVHAHSPFAAG
                     KLALRVSKEKHIPLIATFHSKYREDLARAIMIKPIVDDQIKRIVDFYYSADHVWVPQE
                     SVARTLREYGYKGPYDVVENGVDFELPENIAPYRARGADYLGLPEEYLVGLFIGQHIL
                     EKNLEFLLRTLPLIMDELPNFRMIFVGKGYAKESLIALAQKLDISDRVVFHDVIYDRE
                     LLKAIYARADLFLFPSLYDNAPLVLREAAAMKTPAVLIKGSTAAEVIQNGENGFLCQA
                     DNEDFAQTIVRALSDREALARIGANAQRTLCRTWESIIDEVAQRYHSILDHWTR"
     gene            complement(4131..5264)
                     /locus_tag="PLS88_06410"
     CDS             complement(4131..5264)
                     /locus_tag="PLS88_06410"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU07009.1"
                     /translation="MKKKILILMLEVGLGHKIPALAIKEELEMQYPDAFDIAVVDFAK
                     EVGAARTDRQLNISWDIALKFPVFVRIGYRYMEMNRKNFFYIEMLYRDFIQKGMQYIA
                     NTNPDIVLATHALCTYIASKARRIYGGNYKILSYVVDPFDGYSLWANPESDMFLVATE
                     QSKSRLLDHGIKDSNIIITGFPTRRAFFDLKKSKENIFSELALDFTKITILISAGSQG
                     ISNVYSFAKTAKRKNIPLNLIVVTGKNQKLKRNLDNLVHIKNEMNFAVLSYVDNMNEL
                     IAASDVVAGKAGASTFMEAMFMKKPMIFTGWTAYNDWYIIKFALDKQIGWYCPNLASF
                     LNILKKLSKEPYTVLEYQRRIEALRFVPGTKAISEIIAGIAGQ"
     gene            complement(5354..6001)
                     /locus_tag="PLS88_06415"
     CDS             complement(5354..6001)
                     /locus_tag="PLS88_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOU07010.1"
                     /translation="MSRRYVVVAGNIGAGKSTLVSLLAEHLGFIAYFEPVLENPFLKD
                     FYADMPRWAFHSQLFFLSHRAEMHRKLSEDPHSVVQDRSIYEDAEIFAKNLYIQGIMA
                     EREWCIYQALYATLAEILPHPSLVIYIRASVSTLQSRIKQRGRDFESSIPDSYLEGLN
                     ELYEEWISSFTLAPILTIPGDSLDFVAESRDLQVILRTIIKRLEDNQPYLFPYEM"
     gene            6175..7527
                     /locus_tag="PLS88_06420"
     CDS             6175..7527
                     /locus_tag="PLS88_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07011.1"
                     /translation="MKRFLVLLFACLAAVASIGAQQAPAVLSAYSDAEPQTKEIIDAA
                     RLLESKGQYAKAWNLLAAFDPDSKDGYVLAEKIRLALEDSIDNGMFVSFSFIDLEEGQ
                     SIYEAFLNPPEKQTIVDFNPLDLVEELEESGEAIPPILSLEFAGYLYTVNQEYGDNWL
                     IDSFTLQQKAVENYDRALAYGLYTDKSLANHAELLINLQQYEGAEKVLRQAISLFPED
                     QSYAVSLASSLNDQGKFDQVYPVIDEVIAEPDVTNSTYNAYVEGIKAGLNSGDTQKTD
                     LYVDAMIERFPDEYAPMLIQHLVAVIMGETEKANISADNVTEKFSVNPDVVQSLLSTW
                     LSASDPVSGFDYLNRSLEKYTNDDQAMGTLLFYRALMYAQTARSADDLKLAISDMEET
                     EKRFGAVFEKEDPVFQTIGQLITEWKQIIEQLQVNDSAASPEQSTPSEDANTASGTSN
                     "
     gene            7551..8747
                     /gene="nagA"
                     /locus_tag="PLS88_06425"
     CDS             7551..8747
                     /gene="nagA"
                     /locus_tag="PLS88_06425"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOU07012.1"
                     /translation="MQSVFCIHNATVIAGYARMDRSAVLVEGDSIADVFSERRFAQKS
                     FGSQVHLIDAGGACLTPGLIDTHLHGIGGFGTEDLSIESILGMSRMLPAWGVSSFVPT
                     MYPMAEEDMIRAIRAVVLAMGHEEGAKIIGIHIEGPFISPAQLGVQRIEFIQPVDLGL
                     MQRLWDASEGHIVSMTVAPELKGMRELALFCNQLGIVLQAGHTDASYENMIEGMQAGI
                     RHSTHMFNAMSRLHHRNPNAVGAILIQPDLSCEIIADGLHVHPDLIRLLFRDKPEGNI
                     VLVTDSLKPTKQAANTPMFANDEEVYFENNLFYRARDGVIAGSSLTMIEGVRNLVRFG
                     VPLESAVKMASANPARIFSLGRCGMISPGYKADLVLADMNFEVQMTIIDGKIKYDRNA
                     LERGIL"
     gene            8744..9547
                     /gene="nagB"
                     /locus_tag="PLS88_06430"
     CDS             8744..9547
                     /gene="nagB"
                     /locus_tag="PLS88_06430"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOU07013.1"
                     /translation="MRVVIQQSYESMSKWAAHYLVQHINEHGSQKPFVLGLPTGSTPL
                     GMYRELISLCKAGKVSFKNVITFNMDEYVGLPPEHEQSYHRFMWDNLFSHIDIDPKNV
                     HILNGMASDLQKECAAYEKAICDAGGVDLFIGGLGADGHIAFNEPGSSLSSRTRIKTL
                     TRDTKKANARFFGGDPDKVPTQALTVGVGTIMDAYEVMILVSGTEKARALKHGIEMGV
                     NHMWTISCIQLHPKAVIVCDEDATNELRVATVRYFKEIEAPNLRNWNLD"
     gene            9591..10457
                     /locus_tag="PLS88_06435"
     CDS             9591..10457
                     /locus_tag="PLS88_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOU07014.1"
                     /translation="MRSDIKGIMAMVVTSVCWSLSGLFIKLIDWHPITIAGARSFIAT
                     LFILAVVRRPKLSFDKNQIVAAISYSCTMLLFVYANKNTTSANAILLQYGSPVYVMLL
                     SGIMLKEKPLPEQIGALIAIVIGMCLFFADSLSLGHLNGDIAAVLAGMTFAIHTLFMR
                     RQKEGSPIESLLISHGMTAIISLLISIFLPPPIISKRSVAAIFGLGIVQIGFAAVFFS
                     YAIKRISAIQSSLISIIEPALNPVWVYLAIGEKPSRLAILGGVIIISAVVISSIFSVS
                     RSSRALSIDREE"
     gene            10622..11230
                     /locus_tag="PLS88_06440"
     CDS             10622..11230
                     /locus_tag="PLS88_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOU07015.1"
                     /translation="MRQSFLSITILLILITDPLGNIPLFIASLRHVPKERRPEVILRE
                     CLIAFLVLVGFLFFGEIFLSALNLSDDILRISGGVILFLIALNMIFPGTGGKLVEDEI
                     EGEPFIVPVAIPLIAGPSAITYVMLLMKSDPSRALEWVGAIFIAILVTMVAFFMSNKL
                     KEWLGPRALSAIERLMGLVLTAIAIDMLLSGLLQYLSSVQNL"
     gene            complement(11321..11593)
                     /gene="yccX"
                     /locus_tag="PLS88_06445"
     CDS             complement(11321..11593)
                     /gene="yccX"
                     /locus_tag="PLS88_06445"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOU07016.1"
                     /translation="MKAFHAIIKGEVQGVGFRMSAVIRAERLGLKGWVRNNEDGDVEV
                     WAEGEPEALEQFYSWLQVGPAAARVDQVLKTDEVPRGNYTRFSVAF"
     gene            complement(11618..12826)
                     /locus_tag="PLS88_06450"
     CDS             complement(11618..12826)
                     /locus_tag="PLS88_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU07017.1"
                     /translation="MLVEKRFLPLYISLFSIFALFGISMTVIGATLPKIFAEFGWSYM
                     TAGIVIAAGSIGYFLSSYLAGILFPFLGIRLTISLSLILICCGLELFARTPIAWLNMM
                     LYFIIGVGQGGIEIAVDWAVLRMEGPGGGKAMSLMHGAFSIGAVVGPLIIAILISANL
                     EWTLVYRIIGILFIGLAIFMQFMPLRFLGKDRARITNRSRRDLFQQSMYWLGFFALFV
                     YVGVELGISNWIAEYFVSVFKAPIPTGSFMVSLFWAGLLMGRFGIPIIQKSIKREKIL
                     IALSTLMAISLIAFNIIGFIGDGRIASAVAGIMVIFAGFGCSIIYPTIMSIIGDSFPQ
                     AQSEAVGFAAMGGGIGGFLFPYMMSAISTGWGIRTGFAIYAFFSLLMVVLNILLVRAQ
                     EQKSNSISEL"
     gene            12970..13971
                     /locus_tag="PLS88_06455"
     CDS             12970..13971
                     /locus_tag="PLS88_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU07018.1"
                     /translation="MTSIKDIARLAGVSPSTVSRVLNEREYVRDEVRQRVLSIVKEKG
                     YVQNNVARSMVLKRSFTIGVVIPHLFNMFQRQLFASMEFFLEQRGYKTQFFFIRWSEA
                     SEQAFLRRLKSETLDGIIFIHELYSPAVYQHIQERGLPVALCTFEKDQFDFPAVHIEE
                     EASAALATEYLIQRGHRNIGLITGGHFSFGKQREEGYRQALKAHGIPCNPELMVRVPS
                     YNPEAGRSGMITLLTRKLPMTAVFAATDELAIGAIKALYDSGIRVPEEVSVIGLDDID
                     ISTFTTPALTTVRQPIFEMGKKSADLVCDWIARGHMQEKVELFSTTIVERESVRSLA"
     gene            14142..>14323
                     /locus_tag="PLS88_06460"
     CDS             14142..>14323
                     /locus_tag="PLS88_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07019.1"
                     /translation="MICKYKAICALLIIFLGVGGLGYSESKKDPIKVAVSIPPMKEWV
                     NRIAGDRAEVIVIMPPG"
BASE COUNT         3883 a         3122 c         3308 g         4010 t
ORIGIN      
        1 aaaagcattc taagaggaag agcaaaaaat gaataacgaa tcttcgaatg agctatattc
       61 caagcgttat tccatcctaa cagcagttat gcttggtggc attatggggc ccatcgatgc
      121 aagcataatc aatgtttctc ttccatcgat tgctcaagct ttcgatgtga gtattccaat
      181 agcgcaatgg gtgccaatga tatatttgct cgctatcagc agtctgctgc tattctatgg
      241 tcggcttggt gacatgctgg gatataggaa agtatattta tctggccttg ttggattttt
      301 gattgcttct ggtctctgtg cgttttcttc ttcgatatat tggcttattg cttttagggc
      361 tattcaggga ttggctgctg gtatgatgat ggcggttccc tatgctatca taactacttc
      421 ctttccccct gaagagcgag gcaaggcctt gggcataaat gccataagta tctcggccgg
      481 cttggccata ggcccttctc tgggcggttt tataactgct ctattgggat ggagatttgt
      541 cttccttatc aatattccaa taggtatcat tggcttgctc tggggccggc atagtatccc
      601 agaatcgaaa ggacaacctg gaagaataga tgtgcttgga gccttgctgg catttatctc
      661 gctattctcg attctattct taataaatcg atctcatagc gaaggattag cttggctttc
      721 tcctttttta ttggcaattg cgattctagc agggattgga tttatttgga cggagaagag
      781 aaccaatcag ccaatgttac atttagctct gtttcgaaat gtaacattct gctttgccaa
      841 tttgagcgct ctactcaact tcatgtctca gtatgttatg gtttttctta ctcccttcta
      901 ccttcaaagg gttgtccact gcgctcctaa taagcttggc ttgattatga cttcttttcc
      961 actggctgta atggctgtcg ccccattcag tggcagcctt tccgatcgca taggcacaag
     1021 ggttctggcc tgcagtgggg cttcgatctg tgctatatct ctgtttttta tggctggact
     1081 gtcctcttcc gccaattcaa aagatgttat atggagaatg gtattgtttg gaatcggtac
     1141 gggcattttt caatcgccta acaatagcac agtgatggga aatgtaccta gagagcatct
     1201 aggagtagct tcgggtatcc ttgcgacaat gagaaatatt ggtatggcgc ttgggattgc
     1261 aattggaggg gcagtgctct atgcttttgc ccctccatcc ctgcttcaaa agagctttct
     1321 cgaaggttgg gaagccgagg tcttcttgtc cgccttaaaa tatgcttata gttttggtgg
     1381 tgctttatca gtggcagctg catttgcttc tttagtgagg agtcgagctg cttccagcgc
     1441 agctttgtag tttggctcct gtgcgatttc tggaacctgt tccgtataga cgaccttatt
     1501 agctttatcg agcacgacca cggctctcga caaaaggcca gccataggac cagagattat
     1561 ctcgacacca taagccttgc caaaatctct cgttcgcagt tcggagaggg taactacctt
     1621 atctatgccc tccgcttcgc aaaagcgctt ctgtgcaaag ggcaggtccc tgctgatggt
     1681 caagatgacc acatcgtcaa gagaagatgc tgccttgttg aatgcccggg cactcgcggc
     1741 gcagacgcca gtatctaggc tgggaactat attgagaatt ttgatctttc cttcgaaatc
     1801 agcaaggccg acatcgctta gatcggattt cgttagccta aagtctggag ctgtggttct
     1861 tttagcggga agagagccta ttgtctggat tgggttgcct ttgaatgtga tatttgccat
     1921 ttttatgctc ctttggatat caatttacat caaatatatt aaaaaccata tattgtcgtc
     1981 aagccaagat tgattataat ctcattccag gtttcggatt cttacttcga tctctttatc
     2041 gctggcgagt agccttgtta tctcgctggt atcggtcgaa ccgaaatgat aaggatagag
     2101 aacctttggc ttgattgtgc gagcggcttg agcgacttgg gctggtgtca tggtataggg
     2161 cagattcatg ggcaaaaatg cgatgtcgat tttgccaagg tttgccattt cgggtatcgg
     2221 ctccgtatct cctgctatgt agatatgcag tgagccaata gtgagtacat agccattgtc
     2281 tttcctttct tttggatgat agccaagtct ggacgaagtg atattatagg ccggtactgc
     2341 aagcacattt atacctgcaa catcgatcga atggccatgc tgcaaggcct ctccattgcc
     2401 gagctttttg cgggacgcat ccggcagaat gatgtgtgtt tccggtttgc tgagagcagc
     2461 gatcgctttt ggatcgagat ggtcgccatg ttcgtgtgtt atcaagatta gatcggcctt
     2521 aggatacttg gaaaaattgg catattgact tactggatct atgtagatat tagaaccatt
     2581 gtagactaga agcagagagc catggccaag aaagacaatc tccaatttgc cttttaccgc
     2641 atcgaagaaa tccgattcct tgctttctgg ccagccctgg gcatagcctg tcatcgctga
     2701 aagcgaaaag gccaatacaa tagaaaagag catatttgtc atattgcacc tctctatttc
     2761 tagattaatg ctagcgatat accaaagtca ataaaattca attttatgtg atagtgcagc
     2821 tcctgacaaa tattcaaagc ttaggcgcat agtctttcat tatggagtgc atgataaatt
     2881 ttcgctttat tatgtgccca tttgacgata tggcagatgg gctatattct taacgggtga
     2941 agaaagttac atcaggtctt tttaacgacg gctatcttcc cataatagat ggcgtgaccg
     3001 ttacagtcag gaattatgcc tattggttgc gaaagaagct cggccctacc tatgtagttg
     3061 caccctttgt tcccaattac aaggacaatg atccttttcc ggtgattcgt ttcctttcta
     3121 taccgacaat cgttcgacct ccttatagga ttgggctgcc tgagctggac atgcgcttgc
     3181 cctccatttt aaagaagaga gagttcgaca ttgtacacgc tcattccccc tttgctgcgg
     3241 gcaagctcgc cctgcgagtg tctaaagaaa agcatatccc cttgattgca acctttcact
     3301 cgaagtatag ggaagacttg gctcgcgcca taatgatcaa gcccattgtc gatgaccaga
     3361 taaaacgcat agtcgatttt tactatagtg ccgaccatgt ctgggttcct caagaatccg
     3421 tggctcgaac gcttcgcgag tacggctaca agggtcccta cgatgtagta gaaaatggtg
     3481 tcgattttga gcttccagaa aatatcgctc catatcgagc gcgtggtgcc gattatcttg
     3541 gtcttccaga agaatactta gtgggtctat ttattgggca acatatacta gagaaaaatc
     3601 ttgagtttct cctgcggaca ctacctctta ttatggatga gctgcccaat ttccgaatga
     3661 ttttcgtggg caaggggtat gcaaaggagt cgcttatagc tcttgcacaa aagcttgata
     3721 tttcggacag ggttgtgttt cacgatgtga tctacgatag agagcttcta aaagcgatat
     3781 atgcaagagc tgatctgttt ctctttccct cgctctatga taatgcccct cttgtattga
     3841 gagaggcagc agcgatgaag acaccagctg tgcttataaa aggatcgacg gctgccgaag
     3901 tgatccaaaa cggcgaaaat ggctttcttt gccaggcaga caacgaggat tttgcacaaa
     3961 ctatcgtgcg agcactttcc gatagagaag cattagcgcg cattggagct aatgcgcagc
     4021 gtacgctctg tcgtacatgg gaaagcatca tagacgaagt tgcgcagcga tatcattcga
     4081 ttctggacca ttggacgcga taaaggttgg cgaattgtgt tgcatagcgc ttattgccca
     4141 gcgataccag cgataatctc cgagatggcc tttgtgcccg gtacgaagcg cagagcttcg
     4201 attcgtcgct gatattccag cacggtatat ggttcttttg ataatttctt aaggatatta
     4261 agaaaagaag caagatttgg acaataccag ccgatctgtt tatccagtgc gaacttaata
     4321 atgtaccagt cattgtatgc agtccagcca gtgaaaatca taggtttttt catgaacata
     4381 gcttccatga aggttgaagc gcctgctttt ccagcgacga catcggaagc agcgatgagc
     4441 tcattcatat tgtcaacata gctgagcaca gcgaaattca tttcattctt gatgtgtacg
     4501 agattatcca ggttcctttt cagcttttga ttttttcctg tgaccacaat gagattaaga
     4561 gggatgtttt ttctttttgc agtctttgca aaggaatata cattagaaat accttgagag
     4621 cctgctgaaa taaggattgt gatttttgtg aaatcaaggg caagttcact aaaaatattc
     4681 tctttgcttt ttttaagatc aaagaaggcg cggcgggttg ggaaaccagt gatgatgatg
     4741 ttcgaatctt ttatgccatg gtcgaggagc cggcttttcg actgctcagt tgctacgagg
     4801 aacatatctg attcagggtt tgcccagagg ctatatccat cgaaaggatc caccacatag
     4861 gatagtatct tgtagtttcc accgtagatt cgtcttgcct tagaggctat ataggtacag
     4921 agagcgtgtg ttgccaagac aatgtctggg ttggtgtttg caatatactg cattcctttt
     4981 tgaatgaaat ctctatagag catttcaata tagaagaaat tctttctgtt catctccatg
     5041 tagcgatacc ctatacgaac gaagactggg aatttaagcg caatatccca gctgatattg
     5101 agctgacgat cggttctcgc ggctccgacc tcttttgcga aatcgaccac agctatatca
     5161 aaagcatcag gatattgcat ttcaagttct tcttttattg cgagagctgg gatcttatgt
     5221 cccaacccca cttcgagcat aaggatcagg atttttttct tcatctcagc tattagttct
     5281 ccttctaggg aataatatca ttcatagcga tgcatatttt tgcatacatg ggagtattac
     5341 aaaagaaagt atctcacatt tcataaggaa acagataagg ctgattatct tcgagccgct
     5401 taattattgt cctcagaatt acctgtagat ccctcgattc tgccacaaaa tcgagagagt
     5461 ctccaggaat agttagaatt ggagcaagag taaaagagga aatccactct tcgtagagct
     5521 cattgagtcc ctcgagatac gaatcgggaa tcgacgactc gaaatcccgc cctcgctgct
     5581 tgatcctcga ctgcaaagta gatacggaag cacggatgta tataacaagg cttgggtgag
     5641 gcaaaatctc ggcgagcgtt gcatataagg cctgatagat acaccattcg cgctcggcca
     5701 tgattccttg gatatagaga ttcttggcaa agatctcagc atcctcgtat atcgatcgat
     5761 cctgaactac agaatgtggg tcttcgctca acttacgatg catttcggct cgatgggata
     5821 ggaagaaaag ctggctatga aatgcccatc gaggcatgtc tgcataaaaa tcctttagaa
     5881 aggggttttc caggacgggc tcgaaataag ctataaatcc tagatgctcc gcaagcaggc
     5941 ttacaagtgt ggattttcct gcaccaatgt tgccagctac caccacatat ctgcgactca
     6001 ttctaatcaa agactaatcc ttagatagcg aaggcgcaag cctatgacta aggtgccttt
     6061 ggttgagaag cacagtatag cattgcagcc taccgttgct ttttaggaca aagataagta
     6121 ttctctctct atagtacgag agaatatgcc cctcaatggg aaggagtttt attcatgaaa
     6181 cgctttttag ttttattatt tgcgtgcctt gccgcggttg cctctattgg tgcgcagcag
     6241 gctccagcag tcctgtcagc ctattcagat gctgagccac agacgaaaga aatcatcgac
     6301 gccgctcgtc tcttggaatc caaaggtcaa tatgcaaaag cttggaatct gctcgctgcc
     6361 ttcgatcctg acagcaaaga tggttatgtt ctcgccgaga agattcgcct tgctctggaa
     6421 gacagcattg ataatgggat gtttgtcagt tttagtttca tcgacttaga agaagggcag
     6481 agcatctatg aagcattttt aaacccaccc gaaaagcaga caatagtgga tttcaatcct
     6541 ctggatttag tagaagagct tgaagaatcc ggcgaagcca taccgcccat tctctctctt
     6601 gaattcgcag ggtacctcta caccgtaaat caagaatatg gtgataattg gctgatcgac
     6661 agcttcacgc ttcagcaaaa agcggttgaa aactacgata gagctctggc ctatggtcta
     6721 tacaccgaca aaagtcttgc aaatcacgca gagcttctaa taaatcttca gcagtatgaa
     6781 ggtgccgaga aggtgctgag acaagctatt tctctctttc ccgaggatca gagctatgcg
     6841 gtaagccttg ctagcagtct aaacgatcaa ggcaaattcg accaagtata tcctgttatc
     6901 gatgaagtga tagcagagcc cgatgtcacc aattccacct ataatgccta tgtggaaggt
     6961 atcaaggctg gcctcaattc tggcgatacc cagaagaccg atctatatgt cgatgcaatg
     7021 attgagcgct ttccggatga atatgcccca atgcttattc agcatcttgt cgcagtcata
     7081 atgggtgaaa cagaaaaagc gaatatctct gcagacaacg ttaccgagaa attcagcgtt
     7141 aatcccgatg tcgtacaatc cttgctatct acctggctta gtgcttcaga cccggtttcg
     7201 ggcttcgatt atctaaaccg ctcgctggaa aagtacacga acgatgatca agcaatgggg
     7261 actctattat tctatagagc acttatgtat gcacagacag cccggtcagc ggatgatctc
     7321 aagctggcta tttcggatat ggaggaaacc gagaagcgct ttggtgctgt atttgagaaa
     7381 gaagatcctg tattccagac tattggccag ctcatcacgg aatggaaaca gattatagaa
     7441 cagctccagg tgaatgatag cgcggcgtcc ccagagcaga gtactccttc cgaagatgca
     7501 aatacggctt ctggtacgag caactgaact gatatttgaa gacagaggct atgcaatcgg
     7561 tcttttgtat tcacaatgcc actgtcattg cgggctatgc gcgtatggac cgcagtgctg
     7621 tgcttgtgga gggagattca atcgccgatg tcttttctga gcggagattt gcgcagaaat
     7681 cctttgggtc tcaggtacat ttgatcgatg ccggaggcgc atgtcttact cctgggttaa
     7741 tcgacaccca tcttcatgga attgggggct ttggcacaga agacctgtcc atcgaatcca
     7801 ttcttggaat gagcaggatg ttaccagcct ggggagtgag ctcttttgtc cctacaatgt
     7861 atcccatggc agaagaagat atgattcggg caataagagc agttgtttta gcgatgggcc
     7921 atgaagaggg agctaagatt attggtatcc atattgaagg tcccttcatc tcgcctgcac
     7981 agcttggtgt acagagaata gagtttatcc agcccgttga tctggggctt atgcagcgcc
     8041 tctgggatgc atccgaaggt catattgtca gcatgaccgt agcacctgaa ctaaaaggaa
     8101 tgcgtgaact agcactattc tgcaatcaat tgggcattgt gcttcaagca ggccataccg
     8161 atgccagtta cgaaaacatg atcgaaggaa tgcaagctgg aataaggcac tctacacaca
     8221 tgttcaatgc aatgagtcgg ctgcaccata gaaaccccaa tgcagtcgga gctatcctta
     8281 tccagccaga tttatcttgc gagattatag cggatgggct tcatgttcac cctgatctga
     8341 ttcgcctact tttccgagac aaaccagaag gcaatatcgt gctggtgaca gactcgctaa
     8401 aaccgacaaa gcaagcggcg aatactccca tgtttgccaa tgatgaagaa gtctattttg
     8461 aaaacaacct cttctacaga gcaagagatg gggttattgc cggaagttct cttacaatga
     8521 tcgaaggggt acgaaactta gttagattcg gagtgcctct tgagagcgca gtaaagatgg
     8581 caagcgcaaa tccagctcga atatttagtc ttggaagatg tggaatgatt agccctggat
     8641 ataaggcgga tctcgttcta gcagatatga acttcgaggt tcagatgaca ataatcgatg
     8701 gcaaaataaa atatgaccga aatgctttgg aaaggggaat cctatgagag ttgtaattca
     8761 gcagagctat gaatcgatgt caaaatgggc ggctcattat ctagtacagc atataaatga
     8821 acatggctca cagaagccat ttgtgcttgg ccttccaaca ggttccaccc cgctcggaat
     8881 gtatagagaa ctgatcagcc tttgcaaagc cggaaaggtc agttttaaaa atgtaattac
     8941 attcaatatg gatgaatatg ttggccttcc gccagaacac gagcagagct accatcgttt
     9001 tatgtgggat aatctttttt cccatataga tatcgaccct aaaaatgtac acattctcaa
     9061 tggcatggct tcagacctac aaaaagagtg tgctgcttac gaaaaagcga tatgcgatgc
     9121 cggtggagtc gatctattta tcggaggcct cggagcagac gggcacatcg ccttcaacga
     9181 acctggttct tccctgtcat cgcgcacaag gatcaagaca ctcactcgag atacaaagaa
     9241 agccaatgcc cgcttttttg gtggagatcc agataaagtc cctacgcagg ctcttaccgt
     9301 gggcgtggga acaataatgg atgcatatga agttatgatt cttgtatcgg ggacagaaaa
     9361 ggcaagagcg ctcaaacatg gaatcgagat gggcgtaaac catatgtgga caataagctg
     9421 cattcagctg cacccaaaag ccgtcatagt ctgtgacgaa gatgccacaa atgagttgcg
     9481 cgtagccaca gtacgctatt tcaaggagat cgaagcgcca aatctaagaa attggaattt
     9541 ggattgaagc tggaaacatc agcttaggtg agcttaggtg taaaaatctt atgagaagcg
     9601 atatcaaagg cataatggcc atggtggtaa cttctgtctg ttggagtttg agtggccttt
     9661 ttataaaact catagattgg catcccatta cgattgctgg agcaagaagc tttattgcta
     9721 ccctttttat tcttgcggtg gtgcgtaggc caaaactaag cttcgacaag aaccaaatcg
     9781 ttgccgcgat atcctattca tgcaccatgc tcttatttgt ctatgcgaac aagaatacaa
     9841 caagcgccaa tgctattctt ctgcaatatg gttcgccagt ctatgtaatg cttctttcag
     9901 gtattatgct taaagaaaag cctcttcctg agcagatagg tgcactgatt gcgattgtaa
     9961 tagggatgtg cttattcttt gccgattcgc tcagccttgg tcatttgaat ggcgatatcg
    10021 cagcggtctt ggcaggtatg acttttgcaa tccatacgct atttatgcga cgacagaagg
    10081 aaggctcgcc tattgagtcg ctgcttatat cccatggtat gacagccatc atttcgcttt
    10141 tgatttctat tttcctgcct ccgcctatta tcagcaaaag gtctgtggct gctatttttg
    10201 gtcttggtat agtccaaata ggttttgcag cggtgttttt cagttatgcg ataaagcgca
    10261 taagcgccat ccagagttct cttatatcca taatcgaacc agctttaaat cctgtgtggg
    10321 tttatctggc cattggcgaa aaaccttcga gattggctat attaggcgga gttataatca
    10381 tttctgcagt agttatatct tcgatattta gtgtctctcg cagctcgcga gctctgagca
    10441 tagatagaga agaatagata gagaagtata gatataggag tataaagcga tcttgcatag
    10501 ggccaggcat cgataaagct atataatgca aataattgtg ctgttagcta gtcaagatgc
    10561 caataatcta ttttgctgat atgactgcga atatgaatca aggaggctga agctaaaagc
    10621 tatgaggcag agttttcttt ccattacaat cctgctcatt ctaattaccg atcctttagg
    10681 gaatattccc ctttttattg catccttgag gcatgtacct aaggaacgtc gtccggaggt
    10741 aatcttgcgc gagtgtctca tcgcttttct ggttttggtg ggatttcttt tctttggtga
    10801 gatattctta agcgctctga atctatcgga tgatatcttg cgtatatcgg gtggggtaat
    10861 cctttttttg attgctctga acatgatttt cccaggaact ggcgggaagc ttgtcgaaga
    10921 tgaaatcgag ggcgagccat ttattgtgcc tgttgcaatt ccattgattg cagggccaag
    10981 cgccattacc tatgtaatgc tcttgatgaa gtccgatcct tcgcgggcgc ttgaatgggt
    11041 tggagcgata ttcatagcta ttctggtaac gatggtagcg ttttttatgt cgaacaaatt
    11101 gaaagagtgg cttggcccaa gggctctttc ggcaatcgag agacttatgg gtttggtgtt
    11161 gaccgcaatt gcgatcgata tgctccttag cgggcttttg caatatctga gtagtgttca
    11221 gaatctttga catattttaa attacggcat attgttctaa ccttaatctc aaatgttcct
    11281 caaaaaacag agctacatca aaccttggca ataggaataa tcagaaggct accgaaaacc
    11341 ttgtgtagtt gccccttggc acctcatcgg tctttaggac ttgatcgaca cgcgccgcag
    11401 caggacccac ctgaagccaa gaatagaact gctctagggc ttcaggttcg ccctctgccc
    11461 atacttccac atcaccatcc tcgttatttc gtacccagcc ttttaaccct aatctctcag
    11521 cacgaatcac cgcggacata cgaaagccaa cgccttggac ttcgcccttt attatcgcat
    11581 gaaatgcttt catgctacgc ttcctatcct atataagtca aagctccgat atactattac
    11641 ttttttgctc ttgggcgcgc acaagcaaga tattgagaac gaccataaga agcgaaaaga
    11701 atgcataaat tgcaaagcct gtacgaattc cccaccctgt cgagatagcc gacatcatgt
    11761 atggaaataa aaatccacct atgccaccac ccattgctgc aaaacctacg gcctcgcttt
    11821 gagcttgagg gaaagaatca ccaataatac tcattatggt gggatagata atagagcaac
    11881 caaaaccagc aaaaataacc ataataccag cgaccgcact cgcaatcctt ccatctccaa
    11941 taaaaccgat tatattgaaa gcgatcaaag aaatggccat cagtgtcgat agagctatca
    12001 aaattttttc tcttttaatt gatttctgaa ttattggaat tccgaaacgc cccattagca
    12061 gcccagccca gaatagagag accataaagg agcccgtagg tatgggggct ttgaaaacac
    12121 tcacaaaata ttctgcaatc cagttagaga taccaagttc cactcctaca tagacaaaca
    12181 aggcaaaaaa tccaagccaa tacatcgact gctgaaaaag atcacgcctc gatctattgg
    12241 ttatgcgtgc cctgtctttg ccaaggaatc ttaaaggcat aaattgcata aaaattgcta
    12301 ggccaataaa taatatgcct atgatccggt atacaagagt ccactccaag ttagcgctta
    12361 tcaagatagc tattataaga ggtccaacta cggctccaat tgaaaaagca ccatgcatta
    12421 ggctcatagc ctttccgcct ccaggacctt ccatgcgaag taccgcccag tctaccgcaa
    12481 tttcgattcc accctgacct actccaatta taaagtagag catcatgttg agccacgcga
    12541 taggggttct ggcgaagagt tctagtccac aacaaataag gatcagcgat agggaaattg
    12601 ttaggcggat cccaaggaac gggaaaagga ttcccgcaag atagctcgat aggaaatatc
    12661 caatcgaacc cgcagctata actattcccg cagtcatata agaccagccg aattctgcaa
    12721 aaatttttgg aagcgttgct cctatgactg tcatggaaat accaaacaag gcaaagatac
    12781 taaagaggct tatgtagagg ggcaaaaagc gtttttcgac aagcatgatg ctacattgtg
    12841 cgatattcgt tcagtcgtcg tcaataagag gctaaagacg gcgatgcgat tggtttcgcc
    12901 aaaatttgtc gaacaagccc taagcataat ctctagatga aaaccttttc agaggctatg
    12961 ataaaataaa tgactagcat caaagatatt gccagacttg ctggcgtctc accttctacc
    13021 gtttcgcgcg tcctaaacga aagagaatat gtgcgcgatg aggtacgcca acgcgtgctc
    13081 agcattgtca aagaaaaagg atacgtgcag aacaatgtag cgcggtctat ggtactcaag
    13141 agaagcttta cgattggcgt ggttatccct catctgttca atatgtttca gaggcagctc
    13201 tttgcttcga tggaattctt tcttgagcag cgaggttata aaacccagtt ctttttcatt
    13261 cgatggtctg aagccagcga acaagcattt ctgcgccgcc tcaagagcga gacactagat
    13321 ggcattatat tcatccatga gctctatagc ccagcggtct atcaacatat ccaagagagg
    13381 ggcttacctg tagcgctctg caccttcgaa aaggatcaat tcgattttcc tgcggttcac
    13441 atagaagaag aggcttccgc tgctctggcg accgaatacc ttatacagag aggacatcgc
    13501 aatatcgggc tcattacagg agggcatttc agttttggca aacagcgaga ggaaggctat
    13561 cgacaagcac tcaaggcaca cggcattcct tgcaatccag agctcatggt tcgcgttcct
    13621 tcctacaacc ccgaggctgg ccgctcgggt atgataacac tattgactag gaaattgccc
    13681 atgacggcgg tgtttgctgc taccgacgag ttggcgatag gagcgataaa agcgctttat
    13741 gattcgggca ttagggtgcc cgaagaggtt agcgtaatcg gtctcgacga tatcgatatt
    13801 tctaccttta cgactcctgc cttaaccact gtccgtcagc cgatcttcga gatgggcaag
    13861 aagagcgccg atctagtatg cgattggatt gctcgtgggc acatgcaaga gaaagtagag
    13921 cttttttcga cgacaatcgt cgagcgcgaa tcagttcgct ctctggctta gagtgatttg
    13981 aatcctcagt agctcaaagc cttaaaaggc cctcgaaaag acttttggaa aggccttcgg
    14041 acaagtccag aaaagctctc aaaaaggctc aaagttctga actgttatta tctttttgca
    14101 aaccacttgc agaaacctct tcttcttggt atattttgca aatgatttgc aaatataaag
    14161 cgatttgcgc gctactgata atttttctag gggttggagg gctagggtat tcagagagca
    14221 aaaaagatcc tatcaaagtc gcggtgagta ttccgccaat gaaggaatgg gtgaaccgta
    14281 tcgcggggga cagagctgaa gtcatcgtca taatgcctcc agg
//
LOCUS       DAOSOB010000052        14036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_4366_length_14036_cov_78.654030, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000052 DAOSOB010000000
VERSION     DAOSOB010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 14036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14036
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4366_length_14036_cov_78.654030"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..895)
                     /locus_tag="PLS88_06465"
     CDS             complement(59..895)
                     /locus_tag="PLS88_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOU07020.1"
                     /translation="MNLVPEKKTNPAIEQVIGVISGKGGVGKSMVASLIAQSFAARGL
                     KVGMLDADITGPSIPRLLGIDCFRGESDGEHLYPVQNEEGIKVLSINLFNENEDEPVI
                     WRGPLLAKAIDQFWSDTIWGDLDYLIIDFPPGTSDVALTAFQTIPFSGLVVVATPQDY
                     VSMIVRKSVNMASMLKAPVLGVVENMRTMVCPHCGKEVALFDDGSQNGAKRMGLPLLA
                     SLPWRKELAQARSLRWSALSEEVKRDADRLANEVEFALAAFSPHSPNSPNSPQQETTS
                     SE"
     gene            complement(1011..2078)
                     /locus_tag="PLS88_06470"
     CDS             complement(1011..2078)
                     /locus_tag="PLS88_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU07021.1"
                     /translation="MSHYKNLVFKGGGVRGVAYIGALQYLYEHGMMRHIERVAGTSAG
                     AITALGVAMNPTDFMSFKTLANSLDYRKVPSEQDEKTEKLTSKRLIAIIEHYRELAIF
                     KNLQCSIRLLQEKGWYSSEYLYTWLRSIIAEQFTVTKDAYTFADFANPQIHKDDRPFL
                     DLYITGTDVSNRTSRLFSFETTPDMEVALAVRISMSIPLFFEAIQYQYPGTEEPQFYV
                     DGGVMWNYPINLFDDHKYCRKLNDGANTETLGLFLFSSSEKTQYPPIKSMLDYMESLF
                     ESVSTVQEQLAIRTEKNYSRTIYIDDCGIDSTDFDIEPEDERYRKLVESGYKATKEFF
                     ESKTEWSQFFAFLKDRFGWKE"
     gene            2350..3150
                     /locus_tag="PLS88_06475"
     CDS             2350..3150
                     /locus_tag="PLS88_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOU07022.1"
                     /translation="MNTENHVMQTFLATLFTWGVTALGAAMVFFFKEINKKVLDAMLG
                     FAAGVMIAASFWSLLSPAIELSESMGLVPWLPAVIGFLLGGFFLLGVDHLLPHLHIGA
                     EMNEAEGLHTSWERSILLVLAITLHNIPEGLAVGVGFGAVAAGIPSASLSGAIALAIG
                     IGLQNFPEGAAVSIPLRREGLSRWKSFVYGQASGLVEPIAGILGAVLVLAMRPILPYA
                     LSFAAGAMIYVVAEEVLPEASRDSNGHLATIGTMCGFALMMLLDVALG"
     gene            complement(3249..4880)
                     /gene="pruA"
                     /locus_tag="PLS88_06480"
     CDS             complement(3249..4880)
                     /gene="pruA"
                     /locus_tag="PLS88_06480"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOU07023.1"
                     /translation="MNNGIFHIPRPINEPVLNYAPGSLERAALKKELDRLRGNQIEIP
                     LIIDGKELRTGKTAPIRRPDVHSEVLGVYHLAGEAEAKMAVEAALAAQEKWAAMPWEE
                     RAAVFLRIAELISGKYRALMDAATMLCQSKTAHQAEIDAVCETIDFLRFNPWYMEQIY
                     SQQPDNAKGEWNRSAYRPLEGFVYAVTPFNFTSIATNLPTSPAMMGNTVVWKPASNAV
                     YSNYMLMKLYMEAGLPPGVINFVPGSGSVISNAVLASPKLAGFHFTGSTEVFRSLWKQ
                     ISLNLDHYRTYPRIVGETGGKDFIFIDPSAELAPTVASTLRGAYEYQGQKCSAASRLY
                     IPASRASEFIDALVAQISAIPMGEITDFRNFMGAVIDETAFENIHGYIERAKAQGAKV
                     LAGGNCDKSHGWFIEPTLILTEDPKSETMVNEIFGPVLTAYVYDDRDMEAAYRLVDST
                     SPYGLTGAIMATDRERINRALLAFQNSAGNLYVNDKPTGAVVGRQPFGGARASGTNDK
                     AGSMINLLRWVSALAIKENFSPQASWRYPFMEGDD"
     gene            4997..6259
                     /locus_tag="PLS88_06485"
     CDS             4997..6259
                     /locus_tag="PLS88_06485"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HOU07024.1"
                     /translation="MEQGKTLNAFVTIILAGMLFAGIIEGGAQAIQGFLRLQVTPARL
                     VSDFVAIAGVESTLINVSTVGFLGYVFLAINRLKLSGASLAALFTMMGFAFFGKTLFN
                     CLPIMLGVSLSALLVRKKPRDYALIAMFGTAMGPFITFIAFELGVKSVFSLPASFSIG
                     LGIGLILPPIAISMLRLHRGYSLYNMGLTAGFLGLFAASIFHAAGADILPLETWGTTH
                     NPILVALLPFVLLVALFCIVMEDPKNILALFRHSYLDFRKILGMSGRLPSDFSDLVSV
                     KGAFLNMIILGLLFWIFLLVIKAPFNGPVLGGLFTILGFSFFGKHLKNVLPIVIGIMA
                     AIFIFDKDLYAPASLLAILFGTSLAPICGEFGPLLGFVAGFLHLVIVDRSCAWHGGMA
                     LYNNGFAAGLTATLIVSIIDCYRSSKAK"
     gene            6336..7793
                     /gene="sbcB"
                     /locus_tag="PLS88_06490"
     CDS             6336..7793
                     /gene="sbcB"
                     /locus_tag="PLS88_06490"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HOU07025.1"
                     /translation="MAVSILWYDLETFGRNPRHDRIAQCAFIRTNIALEEIAQPIVLY
                     CKLPQDYLPDPEACYIHGITPQEVEEKGLSEYEFSLQIQREMSVPGSTVTGYNSIQFD
                     DEFIRYLFYRNLLDPYSREYKNDNSRWDVINLMRAVHDLKYEGFEWPINGGGNPSFKL
                     ADLAKANNVTHENAHDAFYDVQATIGLARKVLETYPKLFEWYYRHRRRENLARLVDLS
                     RRENMLVHTSQRYTRQGGCSTVVAPLGLLEEDRHALVTYDLRFDPKPFLDMSEDELRD
                     LLFSERQEDEQARPPLNMVRLNSCPYLAPLTVLDRQARARLGIDFTECRENLHTLEVN
                     PDLKARFIAAYRFERKSVDEFDPEHALYAGGFYSDEDRRELERFHRVLKASGPLAAKN
                     EFFRIQFQDPRIPTLMGRLLARNFSETLTEDQHLSWKKHVSGWLQLPQEKGAAALADY
                     VRLEKELQEMSPDDPRKPIAAALIRWKDRLSRQAI"
     gene            7994..8485
                     /locus_tag="PLS88_06495"
     CDS             7994..8485
                     /locus_tag="PLS88_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07026.1"
                     /translation="MKCRWFFAIFLVLAGLGIVSAQEKPLETTMLIRVEKTAVRAEPS
                     FASPALSFASYKTPVAIISIENDWVFGFVRGLNRPGYIHMSALSPVKVSLNPKDVSDP
                     PALQDNEIVLAGKGFSSSLELALKESNAFNFEAVDAMVKNSYSYAECLAFIHGIDILQ
                     GEP"
     gene            8487..9575
                     /locus_tag="PLS88_06500"
     CDS             8487..9575
                     /locus_tag="PLS88_06500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOU07027.1"
                     /translation="MRKIAPKSRALVVIFLLILTIPFLVSCTISTESLQFFLDQAILQ
                     GMITPEQSRLILEAVRSFQVESEPFTYEEEYAIGRVVAAAVLSQYQVYDQPDLTAYIN
                     KIGQGLAFYSVRPCLPQGYHILVLDSEEAHAFAAPGGFILITRGLLKLADNEDMLAAL
                     LAHEISHTALGHGLLSLTSYRMGDLVKYYVMLSAESVQNYNKEAMELFLAAVHDFVSL
                     ISVQGYSQVMEFEADSEAIRMLYEANYRTSALGDFVVALGNSKDEHFQQYSMQHPLTD
                     DRLSRIASLDQYYSQQSELEHRKECAQRLDSLSRIEGQSKGNGYPVSDSDLGSLDSEC
                     ELGDGRLISPVSLELLRRERFEAIRPLF"
     gene            9597..11705
                     /locus_tag="PLS88_06505"
     CDS             9597..11705
                     /locus_tag="PLS88_06505"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU07028.1"
                     /translation="MKYMTEAGRHVASHGGFRSTSTLNKARRFFYALLAGVCGIFIFQ
                     WTGVSKKAGMALYDQEMRLLVEAEKKKEDKTSSETLRKISGSSNLPSFSSSDNPKDKV
                     PIALVMIDQSSLDWVQKELGLGWPWPRELYGIIASSLCKADVQAYDILFTEPSTYGPE
                     DDSRCAEAMSSAGNVVLATLTDRKPVLAVSNALCGHVVACVDSDGVCRQYQVSIDTEA
                     GKMPSLGLAVISTLDKTNSISKEKEVSSIGSFEPLEPLESFKPTVFLKFEDSSRFERY
                     SAAQVLASSLDVAGTNSAGLDLDMNFEGKIVLIGITAPGLMDRQATPISPVQPGMEVH
                     ATFISNALSSSFIELSPIWIKASIAMLALVFAALFPIMRTRIWTIVGIVAAIIIPIGM
                     SFVLFRNLVFYNAIPALAAGLFAFVAAVVLGYQAEGRQRAYLRKAFAQYLSPEVISEL
                     IEKPDALRLGGESKVITVLFSDMAGFTAISERLDPERLTLFMNEYLGIISEQILAQGG
                     TLDKYVGDAVVAFWNAPLDIADHALRACLAACRIQEKLIEIGPELENRFGVAPRTRIG
                     IATGSAIVGNLGSNCRFAYTAVGDSVNIASRLETANKVLGTTILTMRETVAAAFAEGS
                     KSSSPSSSILATQDNELTPEIRLPQPEGEILLFRRLGPALVEGKLQTIELWELRTERK
                     DNIAQSFTIAPWQETRYFSK"
     gene            complement(11710..12525)
                     /locus_tag="PLS88_06510"
     CDS             complement(11710..12525)
                     /locus_tag="PLS88_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOU07029.1"
                     /translation="MANRIAVESVTNPFAFEPGESSLVMFHPLAKLAFLIAATSSAMH
                     APLGFLVALFATGILLLSKLPRATAGPFFSVIILILFAALVKGIFPGDGRLFNLATLP
                     GSAVYGLRLLSIYVYSRLFYATTRVSEIGDWMTAGLRSLRKIALRKDAKDKTLPKFDP
                     ESPPYRPGSLDIFLDPGMFISLILLFLPRIFNMYRRVAEAAEVRGLSISKKNFRRALL
                     MLEQLISMSLLQSWKISLAMELRSYSPYRSIKLSKFKKLDWILVGISAILFLF"
     gene            complement(12510..13253)
                     /locus_tag="PLS88_06515"
     CDS             complement(12510..13253)
                     /locus_tag="PLS88_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU07030.1"
                     /translation="MAELFRALSLSHIFPDGVTGIDSISLSIEEGEFLLVAGRNGAGK
                     SLLMRHFVGLSKPSAGEILYRGMPIASQIPLIRREVGYVFQDTEAQVFGLTVEEDLAF
                     GPANLCIKGLKLDAAIKEALAKAHLEGMENRSPGTLSGGEKRRLAIAGVLAMRPRCII
                     LDEPFANLDFPSVQEIIELFSALNAEGKTLIVLTHEIEKVFSLSSRLLILDQGRIVFD
                     GLPKKATRELFLQSGLVYPYREGYPWLIE"
     gene            complement(13246..13806)
                     /locus_tag="PLS88_06520"
     CDS             complement(13246..13806)
                     /locus_tag="PLS88_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOU07031.1"
                     /translation="MEGINAKLTQAIMACLFAALISVGAYAAFPIPGTPVPIVLQNMF
                     IMLAGFILGPWWGLVSVIFYLILGSVGLPVFSGGTGGLAKFAGPTGGYLIGYIPAVIV
                     FGLISKLGKGKWIFNLLAGITGMIIVYAVGIAWLKSVLEIGWGKAIATGLIPFLLGDI
                     VKIILASVLTPPILKAMSHIDQQTNG"
BASE COUNT         3602 a         3100 c         3415 g         3919 t
ORIGIN      
        1 aatgagcgat gatggagcct ctagagagcc tttgcagaga caaagtacaa ataaacggct
       61 attcgctcga agttgtttct tgttgaggcg aattaggtga attaggcgaa tgaggtgaaa
      121 aggctgcaag cgcaaactcc acttcattgg ctaggcgatc agcatctctc ttaacctctt
      181 cgctaagggc tgaccatcga agagagcgcg cttgagcaag ctcttttctc catggcaggc
      241 tcgcaagaag aggtaaaccc atacgcttag ctccgttttg gctgccatcg tcgaatagtg
      301 cgacctcttt tccacagtga ggacaaacca tcgtacgcat gttttcgacc acacctagca
      361 ctggcgcttt gagcatcgag gccatattca ccgatttgcg cactatcatg gaaacatagt
      421 cctggggcgt agcaaccact actaagccag agaaaggaat agtctggaat gcggttagtg
      481 ccacatccga agtacctggc ggaaagtcga tgatgagata atccaaatct ccccaaatcg
      541 tatctgacca gaattgatcg atggcctttg caagcaaggg gccccgccag ataacaggtt
      601 cgtcctcatt ttcattgaaa aggtttatgg agagaacctt tatcccttct tcgttttgca
      661 cagggtagag atgctcgcca tcggactcgc ctcgaaagca atcgatgccc aaaaggcggg
      721 gaatgctcgg tcctgtaatg tccgcatcta gcatacccac tttgaggccg cgagcggcaa
      781 aggattgagc aatcaaagat gcgaccattg acttgccgac tcctcctttg ccgctaatta
      841 caccgataac ttgctcgatg gcaggatttg tctttttttc gggaacaaga ttcatgaatg
      901 tactcccaac gctcgaattt tatgcatatg caatattgca tgataaacga tgttgttcaa
      961 ggatagggct tttcgtatgg cgagaagcta ctggatagca ttgtttaagc tcactctttc
     1021 catccaaagc gatccttgag gaatgcaaag aactgagacc attccgtctt ggattcgaag
     1081 aactctttgg ttgccttgta tcccgactcc accagttttc tatatcgctc gtcttctggc
     1141 tcgatatcga aatctgtgga atcgatcccg caatcgtcga tatatatggt ccgggaatag
     1201 ttcttttctg tccgaatggc gagctgctct tgcaccgtag aaacgctttc gaagagcgac
     1261 tccatatagt cgagcatcga ttttattgga ggatattgcg tcttttcgga agaagagaat
     1321 aggaaaaggc ccaatgtctc ggtatttgcg ccatcattga gctttctgca atatttatga
     1381 tcgtcaaaca gattgatggg ataattccac atgactccgc cgtcgacata gaattgaggc
     1441 tcttcggtgc ctggatactg gtactgtatg gcctcgaaga aaagcggaat agacatcgaa
     1501 atgcgaacgg cgagtgcgac ttccatatcg ggggttgttt cgaaagaaaa gagcctcgaa
     1561 gtccgattag atacatcggt cccggtgatg tacaggtcta ggaagggtct atcatcttta
     1621 tggatttgcg gattggcaaa atctgcaaag gtataggcat ctttagttac agtgaattgt
     1681 tcggcgatga tagagcgaag ccatgtatag agatattcgc tcgaatacca gcctttctcc
     1741 tgtaaaagcc gaatggaaca ctgtaggttc ttgaaaatgg caagttcgcg atagtgctcg
     1801 atgattgcga tgagacgttt gcttgtaagt ttttcggttt tctcatcctg ttctgaagga
     1861 accttgcgat aatcgagact attggccagc gttttgaagc tcataaaatc tgtcggattc
     1921 attgcgacac cgagggcagt tatcgctccg gctgaagtac ctgcaactct ctcgatatgg
     1981 cgcatcattc catgttcgta gaggtattga agcgctccta tatatgcgac cccgcgaacc
     2041 ccgcctccct tgaagaccag attcttataa tgcgacatag gcttctccat ctattatgta
     2101 taggctttgc ggcatttttg atatctccta ttgtctttta gtctataaga atagctattt
     2161 ttcatcgggt tctttccaga agcaaaagat ataggcaaca tggaattcag tcaatcttag
     2221 tagattgaat atcgattggt ataaaaagcg gactgtattt tcatatcttt tttgggctag
     2281 aataccacct aaagagaatt gcgcctaaat agaatagacg caaagaaaat tttctctaaa
     2341 gaggcatcca tgaataccga aaaccatgtt atgcagacct ttcttgcaac cctctttact
     2401 tggggtgtga cggcgcttgg cgctgcgatg gtcttctttt ttaaggagat caacaagaag
     2461 gttctcgacg cgatgcttgg ctttgcggct ggagtgatga ttgcggcgag tttctggtcc
     2521 ttgctttctc cggccatcga attatccgaa tccatgggtc ttgttccatg gctgccggcg
     2581 gttattggat ttttgcttgg aggatttttt ctgctaggtg tagatcatct tttgccccat
     2641 ttgcacattg gagcggagat gaacgaagcg gaaggtcttc atacgagttg ggagcggagt
     2701 atcctgcttg tgctcgccat tactctccat aatatccctg agggccttgc cgttggtgtg
     2761 ggctttgggg cggttgcagc tggtataccc agcgctagtt tatcgggtgc aattgcgctg
     2821 gctatcggca ttgggttgca gaatttccca gaaggggcgg cggtctccat tcctcttagg
     2881 agagaagggc tttctcgctg gaaaagcttc gtatatgggc aagcatctgg ccttgtcgag
     2941 cctattgcgg gaattctagg tgcagttctt gtactcgcaa tgcggcctat tctgccttat
     3001 gcgctttctt ttgccgcagg cgcgatgatc tatgtggtag ccgaggaggt gcttcctgag
     3061 gcaagtcgag attcgaatgg gcatcttgcc accataggta ctatgtgcgg attcgccttg
     3121 atgatgctac ttgatgttgc gctaggctga ttaggcaagg agggcaccta aaataggacg
     3181 cccaaaagag gcacccaaaa cagggtgccc aaaagaaagc gaccaaaaga ggcgccctgc
     3241 atctcccttc aatcgtctcc ttccatgaaa ggatagcgcc agctagcctg cggagagaaa
     3301 ttctctttga tggcgagcgc gctcacccag cgcaaaagat tgatcatgct gcctgcctta
     3361 tcgtttgtgc cggaagctct cgctcctcca aaaggttgcc tgcccaccac tgctcccgtg
     3421 ggtttatcgt tgacatagag atttcccgca gaattctgga acgcaagaag ggctcgattg
     3481 atgcgctctc tgtctgtggc catgatagcg ccggtgagcc cataggggga ggtagaatcc
     3541 acaagccgat aagccgcttc catgtctcga tcgtcgtaga cataagcagt caggacggga
     3601 ccgaatatct cgttgaccat agtctcggat tttgggtcct cggtgagaat aagagtaggc
     3661 tctatgaacc aaccgtgaga tttatcgcaa ttgcctcctg caaggacttt tgctccttgt
     3721 gcctttgcac gttctatata accatgaatg ttttcgaagg ctgtctcgtc gatcaccgca
     3781 cccatgaaat ttcgaaaatc cgtaatctct cccatgggga ttgcggagat ctgagcgacc
     3841 agggcgtcga tgaattcgga agcgcgagat gcaggtatat agagccgaga tgctgccgag
     3901 catttttggc cttgatattc ataggcccct ctaagagtgg aagctacagt gggtgcaagt
     3961 tcggcgcttg gatcgatgaa gataaaatcc ttgcccccag tttctccgac gatgcgcgga
     4021 taggttcgat agtgatctag attcaaagaa atctgtttcc agagggagcg gaatacttct
     4081 gtagagccag taaagtggaa gcccgcgagc ttagggcttg caagcactgc attggaaatc
     4141 acacttccac ttcctggcac aaagttgatg actcccggag gcaaaccggc ttccatatag
     4201 agcttcataa gcatgtagtt ggagtatacg gcattggatg ctggtttcca aaccactgta
     4261 ttgcccatca ttgcaggaga agtagggagg tttgtggcaa tcgaggtgaa gttgaatggg
     4321 gttacagcat agacaaagcc ttctaggggt ctataggccg atcgattcca ttctcctttt
     4381 gcattgtctg gctgttgtga atagatttgc tccatatacc aagggttaaa tcgcagaaaa
     4441 tctattgttt cgcagacagc gtcgatttcg gcttggtgcg cggttttgga ctggcagagc
     4501 atggttgcgg catccatgag ggctcggtat tttccagaaa tgagttcggc gattcttaga
     4561 aaaaccgccg ctcgctcttc ccagggcata gcagcccatt tttcttgtgc tgcaagggct
     4621 gcttccaccg ccatcttggc ttccgcttcg ccagctaggt ggtagacccc caacacctcg
     4681 gaatggacat ctggcctgcg aattggggca gtcttgcccg ttctaagctc ttttccgtca
     4741 ataattagtg gaatttcgat ctggttaccg cggaggcgat cgagttcctt tttcaatgcg
     4801 gctcgttcaa gcgaaccagg ggcataattc aacacaggtt cgttgattgg ccttggaatg
     4861 tgaaaaattc cattattcat gagcagctcc tttttatttc attttgaagg cgctcctggg
     4921 attcgtcaag tcaaatcttt gtaacacttg atttatgcag cttaataatt ctaggatgga
     4981 ataagagagg tgcataatgg agcagggcaa gactttgaat gcttttgtta cgataatttt
     5041 ggccggtatg ctttttgcag ggataataga gggcggagca caagccatcc aaggttttct
     5101 gcggttgcag gtaacacccg ctcggctagt gagcgatttt gtcgcgattg caggagtgga
     5161 gtctacacta atcaacgtga gtacagttgg ctttctgggt tatgtttttc tggcaataaa
     5221 tagactcaaa ttaagtggcg cttctcttgc agctctcttt acgatgatgg gttttgcctt
     5281 tttcggaaag actctattta attgccttcc gataatgctc ggagtatctc tttctgcgct
     5341 cttagtcagg aaaaagcctc gagactacgc cctaatagcc atgttcggga ctgccatggg
     5401 tccatttatc acctttattg ccttcgagct tggagtaaaa agtgtttttt ctttgcccgc
     5461 ttctttttct attggattgg gcattggact catccttcca ccgattgcca taagcatgct
     5521 tcggcttcat cggggataca gcctttacaa tatgggcttg acagcaggct ttttagggct
     5581 ttttgcggct tcgattttcc atgcggcagg tgcagatatt ttgcctctag aaacatgggg
     5641 tacgactcat aatccgattc tcgtcgctct gttgcctttt gttttgctgg ttgctctttt
     5701 ttgtattgtg atggaggatc ccaagaacat attagcttta tttaggcatt catatttgga
     5761 ttttcgtaag attctaggca tgagcggcag gcttcctagc gatttttcag acttagtatc
     5821 ggtaaagggt gcttttctga acatgataat actgggcttg ttgttttgga ttttcttgct
     5881 agttatcaaa gctcctttca atgggcccgt cttgggagga cttttcacga ttcttggttt
     5941 ttcgttcttt ggaaagcatc ttaagaatgt tttaccaatc gtgattggta ttatggcagc
     6001 gatttttata ttcgataagg atttatatgc accagcgtcg cttttggcaa ttttatttgg
     6061 gacatcgctt gcgcctatat gcggagagtt tgggcccttg ttggggtttg ttgcaggatt
     6121 tttgcatctt gtgatcgtag atagatcatg cgcttggcat ggaggcatgg ctttatacaa
     6181 caatggcttt gcggcggggc ttaccgccac tctgatcgtt tctattatcg attgttatcg
     6241 ttcgagtaag gctaagtgag aaaaggccga actgcaatat ttactatcga tgaaaaatca
     6301 tagaagatat tcatagaaaa tagagtgaaa gaaagatggc agtatcaatt ctatggtatg
     6361 acctcgaaac ttttggccgc aaccctaggc acgaccgtat tgcgcagtgc gctttcataa
     6421 ggacgaatat cgcccttgaa gaaatagccc agccaattgt tctatattgt aagcttcctc
     6481 aagactatct tcctgacccg gaggcctgtt atatacacgg catcactcct caggaagtcg
     6541 aagagaaagg cttatccgaa tatgagttct ctttacagat acagcgcgaa atgagcgttc
     6601 caggcagcac agtaacaggt tacaactcca tccagttcga cgatgaattc attcgctatc
     6661 ttttttatag aaatcttcta gatccttata gtcgcgagta taaaaacgac aattctcgtt
     6721 gggatgtaat aaatcttatg cgagctgtcc acgacctcaa atatgaaggt ttcgagtggc
     6781 ctataaatgg tggtggcaac ccatcgttca aacttgcaga tcttgcaaaa gcaaataatg
     6841 taacccatga gaatgcgcat gacgcattct atgatgtgca ggcgacaatc ggtcttgccc
     6901 gcaaggtgct tgagacttat ccaaagctct tcgaatggta ttatcgccac cgacgccgtg
     6961 agaatctcgc gcgtctcgtt gatctttctc gacgcgagaa tatgctcgtt cacacttcac
     7021 agcgctatac gagacaagga ggttgttcga ccgttgtagc tccattgggg ctcttggagg
     7081 aagaccgcca tgctcttgtg acctatgatt tgcgttttga ccccaagcca tttttagaca
     7141 tgtcagaaga tgaattgcgc gaccttctat tctcggaaag gcaggaggat gaacaggctc
     7201 gtccgcctct caacatggtt cgacttaaca gctgtcctta tcttgctcct cttaccgtgc
     7261 ttgatcgaca agctcgagcg cggctaggta tagattttac ggaatgcaga gaaaatctgc
     7321 atactttaga ggtaaatccg gatcttaaag cgcgctttat cgcggcatat cgatttgaaa
     7381 gaaaatcagt agatgaattc gatcccgagc atgcgctata tgccggtgga ttttattcag
     7441 atgaggatcg cagagagttg gaacgcttcc atagagtgct aaaggcaagc ggtccattgg
     7501 ccgcaaaaaa cgagtttttt cgcattcaat ttcaggatcc aagaatccca actctaatgg
     7561 gcaggcttct agcacgcaat ttttctgaaa ccttaacaga agatcagcat ttatcttgga
     7621 agaagcatgt atccggatgg ctgcagcttc cgcaggaaaa aggagccgcc gcgcttgcgg
     7681 actatgtgag attagagaaa gaattgcagg aaatgtctcc ggatgatccg agaaagccta
     7741 ttgctgctgc gcttattcga tggaaggata ggctgagtcg ccaagctatc tgaagattgt
     7801 ttttctgtgc gctatattta catatgtaga ggtgtatata agtaggagcg aatgcgtggc
     7861 ttaagatgaa atcgagtaaa taatcttgtg gctttcttgc tcgcaagttt atatttgaag
     7921 cagggaagca tgataggagt aaggcggtgg ttgcgtcgtt ttatgcgccc atgcgctatt
     7981 aagaaggtgt gccatgaaat gtcgatggtt ttttgcaatt tttttagttc ttgctggtct
     8041 gggaattgtc tcagcgcagg agaagccctt ggaaaccact atgttgataa gggtagaaaa
     8101 aaccgcagtt agagccgagc cttctttcgc ttcgcctgcg ctttcttttg cttcttacaa
     8161 gactccggtt gccataataa gcattgaaaa cgactgggta tttggctttg tacgaggact
     8221 aaataggcca ggatacatac acatgagtgc actttctcct gtaaaagtct ccttgaatcc
     8281 taaggatgtc tcggatccgc ctgcactaca ggataatgag atcgtccttg ctggcaaagg
     8341 cttttcatct agtcttgaac ttgcgctaaa agaatcgaat gctttcaact tcgaagccgt
     8401 agatgcgatg gtaaagaata gctattctta tgcagaatgc cttgccttta tccatggcat
     8461 agatattttg caaggagaac cgtgacgtgc ggaagattgc gcctaaatct cgcgcgctgg
     8521 ttgttatttt tttactgatc cttaccatac catttcttgt atcttgtacc atatcgacag
     8581 agtctcttca gttttttctc gatcaagcca ttttgcaagg catgattacg cctgagcaat
     8641 cacgactcat cttggaagct gtgcgctctt ttcaagtcga atctgaacca tttacctacg
     8701 aggaagaata cgctataggg cgagtagttg ccgctgcagt gctctctcag tatcaagttt
     8761 atgaccagcc tgatcttact gcctatatca ataagatagg gcaggggttg gcgttttatt
     8821 ccgttaggcc atgccttcca caggggtatc atattttggt cctcgatagc gaagaagccc
     8881 atgcttttgc agcgccagga ggatttatat tgataacaag ggggcttctg aagctcgcag
     8941 acaatgagga tatgctagca gctcttttag cacatgagat atctcatacg gcgttgggac
     9001 atggattgtt atcgcttaca agctatcgca tgggagattt ggtgaaatac tatgtaatgc
     9061 tctccgccga gagcgtccag aattataata aagaggctat ggagcttttt ctggcggcag
     9121 tgcatgattt tgtctctcta atatcggtgc aaggctactc gcaagtcatg gaattcgagg
     9181 cagattccga agcgattcgc atgctgtacg aagccaatta tagaacttcg gctcttggag
     9241 attttgtagt ggcattggga aattcgaagg acgaacattt tcaacaatat tcgatgcagc
     9301 atcctttaac tgacgatagg ctatcgagaa tagcaagtct tgatcagtac tattctcaac
     9361 aatctgagct cgagcaccgt aaagaatgcg cacaacggct ggattcgctt tcgcgcatcg
     9421 aaggacaatc aaagggcaac ggttatccag tatcagattc cgatctaggt tccctcgatt
     9481 ctgagtgcga gctcggagat gggaggttaa tcagcccagt gtctctagag ctcttgaggc
     9541 gagagcgctt cgaagccatt cggccgctat tttaggtaat ctcaaaatat cttagaatga
     9601 aatatatgac agaggcaggc aggcatgtag ccagccatgg cggttttaga tctacttcta
     9661 ctttgaataa agcaaggcga tttttttatg cgctgttagc tggtgtctgt gggatattta
     9721 tattccagtg gacaggggtt tcaaaaaagg ctggtatggc gctctatgat caggaaatgc
     9781 gtctgttggt cgaggcagaa aagaagaagg aggacaagac ttcctctgaa actttacgta
     9841 agatttccgg ttcgtcaaat ttgccatctt tttcatcatc tgataatcct aaagacaaag
     9901 tacccattgc gcttgtcatg atcgaccagt ccagcctaga ttgggttcag aaggagctcg
     9961 gtctcggttg gccctggcct cgagaactat acggcataat tgcttcctct ctgtgtaaag
    10021 ccgatgtcca agcctatgat attctattta cagagccttc cacatatggc cccgaagatg
    10081 attctcgctg tgcagaagca atgagctccg caggcaatgt ggttttggca actcttacag
    10141 acagaaagcc tgtgctggct gtatccaatg cattatgcgg ccatgtggtc gcttgcgtcg
    10201 attcagacgg tgtctgcaga caataccagg tttcaataga tacagaagct ggaaagatgc
    10261 cctctcttgg attggccgtc ataagtacgc ttgataagac gaattcgatt tcaaaggaga
    10321 aagaagtctc ttctatagga tctttcgagc cattggagcc actcgaatct ttcaaaccta
    10381 cagtgtttct caagttcgag gattcttctc gcttcgagcg gtatagcgca gcacaagttc
    10441 tagcaagctc tctagatgtg gcaggcacta attctgctgg cttggatttg gacatgaatt
    10501 tcgagggcaa gatagttttg ataggtataa ctgcccctgg actcatggat agacaagcga
    10561 caccgataag tccagtgcaa ccgggcatgg aggttcatgc tacattcata agcaatgcgc
    10621 tctcttcatc ttttatagag ctttctccaa tctggattaa agcttccata gctatgcttg
    10681 ctcttgtttt cgctgctctt tttcctatta tgcgaactag aatttggaca atagtgggga
    10741 ttgtcgcagc aataattatt ccaatcggaa tgagctttgt actttttcga aatcttgtgt
    10801 tctataacgc gattcccgcg cttgcagctg ggctttttgc ctttgtggct gccgtagttc
    10861 ttggttacca agcagagggt aggcagcggg cttatctacg caaggccttt gctcagtatc
    10921 tctctccaga agtgatctcc gagctaatcg aaaaacctga tgctcttagg ctcggtggag
    10981 aatcgaaagt gataacagtg ctgttctcgg atatggcagg ttttacggca atatccgaaa
    11041 ggctggaccc ggaacggcta acgctgttta tgaatgagta cttgggaatt attagcgaac
    11101 aaatattggc tcagggagga actctcgata aatatgtcgg tgatgccgtg gtggcattct
    11161 ggaacgctcc tctcgatatt gcagaccatg ccttgcgtgc ctgtttagca gcctgtcgca
    11221 ttcaagaaaa gctgatagaa ataggtcctg agcttgaaaa ccgctttgga gtagccccaa
    11281 gaaccagaat agggattgcc acgggctctg caatagtagg caaccttggc tcgaattgcc
    11341 gttttgctta cacggctgta ggtgattcgg tcaacatagc tagtcgcctc gaaaccgcaa
    11401 acaaggtatt gggaaccaca atacttacaa tgagagaaac tgtagccgca gctttcgctg
    11461 aaggctctaa gtcaagctct ccttctagct ctatcctagc aactcaagat aatgaattga
    11521 ctccagaaat acgccttccg cagccagaag gagaaatttt acttttccga aggcttggtc
    11581 cagcgctagt agagggaaaa ctccaaacca tcgagctctg ggaattaaga acagaaagga
    11641 aagacaatat cgctcaaagt tttacaattg caccgtggca ggaaacccgg tatttttcaa
    11701 aatagaatct caaaataaaa acaagatcgc cgaaattcct accaaaatcc aatctagttt
    11761 tttaaatttc gacagcttta tactcctata tggtgagtag ctgcgtaact ccattgctag
    11821 cgatatcttc cacgactgta agaggctcat cgagatcagt tgctcgagca taagaagagc
    11881 gcggcgaaag ttcttttttg aaatagataa gccgcgcact tcggctgctt ccgcgacgcg
    11941 acgatacata ttgaaaatac gcggtaagaa aaggagtatc aaggagatga acattccagg
    12001 atctaagaaa atgtccaagg atccagggcg atagggcggc gattctggat cgaattttgg
    12061 taatgtttta tctttggcat cttttctaag ggcgattttt cttaagcttc gcagaccagc
    12121 agtcatccaa tctccaattt cgcttactcg ggttgtcgca tagaaaagtc gagaatatac
    12181 atatatagag agcagcctta acccatagac cgcagatccg ggcaatgtgg ccaggttgaa
    12241 gagtctgcca tcacctggga atatgccttt aacgagagcg gcaaataata taaggataat
    12301 taccgaaaag aaaggacccg cagtggctct cggtagtttt gagagcagca aaattcctgt
    12361 tgcaaaaaga gctaccaaaa atcctagagg ggcatgcatt gccgagcttg ttgccgcaat
    12421 caagaaagcc agcttcgcaa gcggatgaaa catgacgaga gagctttctc ctggttcgaa
    12481 agcaaaagga ttggttacgc tttccaccgc tattctatta gccatggata accctctcga
    12541 taagggtaga ctaagccact ctgcaggaat aattctcttg tcgctttttt cggcaatcca
    12601 tcgaagacta ttcttccctg gtcgagaata agaagtctcg aggaaaggct gaatactttt
    12661 tcgatttcat gggtaagcac tatcaaagtt tttccctctg cattaagtgc actgaagagt
    12721 tcaatgattt cctgcacaga aggaaaatcg aggtttgcga atggctcgtc aagtattatg
    12781 cagcgcggtc gcatggcaag aacgcctgca atagccagac ggcgtttttc tccgccggag
    12841 agagtccctg ggctgcgatt ctccatgcct tcaagatggg cttttgccag cgcttcttta
    12901 atcgcagcat caagctttag gcccttaata catagatttg cgggaccaaa agctagatcc
    12961 tcctcgactg tcaaaccaaa aacctgagct tccgtatcct gaaagacata gccgacctct
    13021 cttcggatca gcggaatttg cgaagcgatg ggcatgcctc ggtagagtat ttctcctgcc
    13081 gatggcttag aaagacccac aaaatgcctc ataagcagcg attttcctgc gccgtttctt
    13141 cctgcaacaa gaaggaattc tccttcttcg attgaaagcg atatcgaatc tataccggtt
    13201 acgccatctg ggaagatgtg actcagagat agcgcccgaa atagctcagc catttgtttg
    13261 ttgatctata tggctcattg ccttgagaat tggtggagtc aagacagacg caagaatgat
    13321 ctttacaata tcgccaagga gaaaaggaat gagtccagta gcgattgctt ttccccaacc
    13381 aatctccaac actgatttca gccaggctat acccacagca tagacaatta tcattccggt
    13441 aattcctgcc aggagattga aaatccattt gcctttgcct agttttgata taagaccaaa
    13501 gacgatgacg gcaggtatgt agccgataag gtaaccgccc gtaggtccgg caaatttggc
    13561 aaggcctccg gttcctccag agaataccgg caagcctacc gaccccaata tcaaatagaa
    13621 gatcaccgac acaaggcccc accacggtcc tagaatgaat cccgctagca tgatgaacat
    13681 attctgaagc acaataggca cgggagttcc agggattgga aatgcagcat atgctccaac
    13741 agaaatcagg gctgcaaaaa gacatgccat gatggcctga gtgagttttg cgttaattcc
    13801 ttccatagag cggactatgg cctaaattca aggccttggt caagtggatt tctttattac
    13861 tgctacgaac cttgtataag aatttgaagc ataaagtagg gaagagagcg ataaagagaa
    13921 tgtatggagg taagcatgca ttcttttcct ttctgcccaa atccagcctg tctctatcat
    13981 aaggcagctc caaaaggtcg ctggttttta aggctaggct tctatcatac caaatg
//
LOCUS       DAOSOB010000053        13960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_4404_length_13960_cov_62.986120, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000053 DAOSOB010000000
VERSION     DAOSOB010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 13960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13960
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4404_length_13960_cov_62.986120"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLS88_06525"
     CDS             <1..452
                     /locus_tag="PLS88_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07032.1"
                     /translation="LQEEIDATLKAGGLDLLEFSLVRHKGGDLHVKAVIYRPEGTGTD
                     ECTKAYRLILPRIQMALGVQDPYIEVYSPGIDRAIRTEREWAAFKNCYIKFMTSESND
                     WKKGRIVHFDNKKVQIEMDDTILNIPIASIIKAKLDSSREGEKVHGI"
     gene            442..1878
                     /gene="nusA"
                     /locus_tag="PLS88_06530"
     CDS             442..1878
                     /gene="nusA"
                     /locus_tag="PLS88_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOU07033.1"
                     /translation="MASEMAEAIHQLISEKGIPEDLIVKTLEEAILAAYKKKFGTNDN
                     AIVRLKENYEGIEIYAKKTIVSDEDFEDEVLEIPLEEAKKLADEAEIGDILEIPIDPS
                     EFDFQSVMLAKQTARQSLRDISRDTLYAEFKSKVGEIVIGYFQRERNGTIFVDLGKIE
                     GIFPRKYQSPRETYHVGDRIKALIVEVEKTKTGFQVVLSRTHAEFVRKLLELEIPEIY
                     DKTIEIFKIVREPGYRTKVAVYAKRSDVDPVGACVGPKGMRSQLISQELEGEKIDFIR
                     YDINPKEFIKNALAPAQVKEVYILDEARHMALAVVDEHELSIAIGKSGQNVKLANRLC
                     DWNIDVMTEEQYLQDSRNVELKKAADRLFSVEEEEGEEFLIRDLPNLEPAWINALESA
                     GIRLIEQFLELDEEEIAQCTTLSARDISAIRDIIEENVEIIQEEVEEEGIVSYSCPEC
                     GSPITIDMDHCPSCGVALSFEAEEEQDE"
     gene            1888..4635
                     /gene="infB"
                     /locus_tag="PLS88_06535"
     CDS             1888..4635
                     /gene="infB"
                     /locus_tag="PLS88_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOU07034.1"
                     /translation="MTESNDKDLKKVHLIKQPRQETPEQPQELPAQNPLDQGAEKKKV
                     VVVVKKKIVTTKKVQAKAMIPEAHEDQKMREKQSTLSAQPLSEAGKTTTQIRTEDKAV
                     SPSSTAPSTTTLSSSKSASTAPSQKSFEHKQEIRQDETIISSQRRYSSDGRAGNLAQG
                     RPSSTYRNGHERGESRPASGSYQGRAHGQPSGRSYPSTSSRSGQFGSQQFPHGSRPSG
                     GSQNLSGTGPSGRAAYPYAKTGQPGGAQRSGGYTGARQSPAGGRYGLRQTPAPIPSTE
                     NERPASRRAPAKKKGVFNKREEETELEKQIQLKKKAEAKLAAVPKSIDIMENISISEL
                     AKKMNLKPADLISKLMSLGVMATINQKIDADTAAILAAEYSCEVKVVSLYDETVIEKA
                     ADKPEELKPRPPIVTVMGHVDHGKTKLLDAIRKTDVVSQEFGGITQHIGAYMVEAPNG
                     KISFLDTPGHAAFTKMRARGANITDIVVLVVSATEGVMTQTKEAIDHAKAANVPIIVA
                     INKIDLPEANPDRVKTQLSELGLIPEEWGGQTQFVEVSALQKIGIPELFDAILIQAEM
                     LDLKANYDRLADGKIIESLIDQGRGIVATIMVQNGTLHIGDPFVAGIFPGRVRAMFDD
                     KGNRIELATPSMPVEVIGFEGMPEAGDPFEVVEDEKFARQISAKRQELKKYEEGRNVK
                     KVTLDNLYETISQGEIKELKVIIKGDVHGSVEALKGMLEKLSTSEVHLNVIRAAAGAI
                     TEDDVMMASASNAIIVGFNVRPTSSAKLLAEREKVDIRKYNLIYRAQEEIKRAMEGLL
                     APELKEQEVGKAEVRNIFRVPKAGIVAGCFVSEGIVKRNCQVRVIRDNIEIFQGNLSS
                     LKRFKDDVKEVAAGYECGIGIENCNDLKEGDILEFYETIEVARTLESGDKNEPGTQAS
                     S"
     gene            4607..4957
                     /gene="rbfA"
                     /locus_tag="PLS88_06540"
     CDS             4607..4957
                     /gene="rbfA"
                     /locus_tag="PLS88_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOU07035.1"
                     /translation="MNQVRKRRLEELIREEISKMITFGEIKDPRVNSFLSITRVEASQ
                     DGSHAKVFVSSLDDIEGHLDEAIIGLSHAAGFVQSIIAKRVRLRLTPVLTFVADRSLK
                     EGFELTEKLKDLLK"
     gene            4957..5895
                     /gene="truB"
                     /locus_tag="PLS88_06545"
     CDS             4957..5895
                     /gene="truB"
                     /locus_tag="PLS88_06545"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOU07036.1"
                     /translation="MQHSGFFLVDKPEGPTSNRVLQDIRKSLSVEFFASQQKFGHAGT
                     LDSFASGLLIILTGKATRLTRWFMNQSKEYDAVFRFGEETDTLDPQGRIVDIGAIPTK
                     ETLENIIPSFCGNIKQIPPSYSAVHVCGKRSYEIARAGKKPEISPRFVTIDTIELQSF
                     KEKEATLRIRCSSGTYIRSLARDIGHACGSCATVVSLRRTKIGPFFISQAHHPHECNE
                     KTLKVFSPDIAQSLGLATAVIQLDFVKHFIHGRALPIKAITFNDDQMTLLRNLDLSFE
                     SKQVALFSEKGELLGIAEHKDKELVPKVVMEEESRL"
     gene            5892..6701
                     /locus_tag="PLS88_06550"
     CDS             5892..6701
                     /locus_tag="PLS88_06550"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HOU07037.1"
                     /translation="MNVLTWEEFQNNYSQEQLAATVGVFDGLHYGHQVLIETVKQQSP
                     ALRSCVITFRENPKKILHPSSYQGSLLTLEQKLSGFQNMGIEYCVMIDFSNNFAKLTG
                     REFLAALYIANVRFLAVGVNFQFGHNLDTNATRVEKLSEEIGLRSCIVKNIMYHSHAI
                     SSSRIRHAVLEGRLGEAAEMLGRPYQILARRERYAIAEKSEYFLPEDDLLIPPEGQYE
                     AFIKDNDTIQETKVNVVHNHLALMRLRMQSEKIRLAFVDKAVQEKEKIVWL"
     gene            6692..6961
                     /gene="rpsO"
                     /locus_tag="PLS88_06555"
     CDS             6692..6961
                     /gene="rpsO"
                     /locus_tag="PLS88_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOU07038.1"
                     /translation="MALSKEDKAQLVLEYGKDAKNTGAIETQIAIISARIAYLTEHFK
                     THKKDTNSRRGLLKLVGQRRKLLKYLQRTDLESYRAIMEKLQIRK"
     gene            7030..9210
                     /gene="pnp"
                     /locus_tag="PLS88_06560"
     CDS             7030..9210
                     /gene="pnp"
                     /locus_tag="PLS88_06560"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOU07039.1"
                     /translation="MINKLECKIGNEILVFETGRMAKQANGAVFATYGGSAVIATICC
                     SPTAIEGLDFVPLTVEYSEKYYAAGKIPGGFIKRETKPKDREILVSRIIDRPMRPLFH
                     KEFGREIQIVPTCISADQINPPDIIAANAASAAVHISDIPFDGPVGCVRVALVEDSYI
                     LNPTYEQIEQAKLEIVVAGTAQGITMVEGGSREASEEEMIQAIEMAKEPISQICSTIE
                     ELKRIAGKEKLALAPLSIELNKKDEIRECAYELLSKALFTKIKQERAKAVSEAISAVK
                     EKFADDLNDEIQMKLFSKLMDDLQYEILRSSILEKGLRVDGRGLEDIRPITCEIGILP
                     RTHGSALFTRGETQVLAVTTLGTVFDEQIFDDIEGDRRERFMLHYNFPPFSVGEVGRL
                     GTGRREIGHGDLARRSIEPMLPPKERFPYTIRVVAEVLESNGSSSMATVCSSSMSLMH
                     AGVPCSKAVAGIAMGLITDETRYAILSDILGDEDHLGDMDFKVAGTKDGITGFQMDIK
                     ISSVSTEILRKALEQAKQGRLHILSIMEKTIAEPQSEISPFAPQVINARIDPEKIGLV
                     IGPSGKNIKALSEKFDVQINIDEDGYLTVYGKDQKSAYSARDAILGMVEEPEVGKIYT
                     GTVKRIMDFGAFIEILPGREGLCHISKLSKGHVDKVSDLLKEGDTIKVKLMEIDHLGR
                     LNLAAVDALDDKGQIAQRDTRRNERPSDRQRDMRSSRHDSHWDR"
     gene            9331..9783
                     /gene="dut"
                     /locus_tag="PLS88_06565"
     CDS             9331..9783
                     /gene="dut"
                     /locus_tag="PLS88_06565"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOU07040.1"
                     /translation="MKPEERAIGVIIALSEGAQMPQYATDHAAGADLYAVLENPLVLE
                     PLSRALIPTGLRIALPLGYEAQIRPRSGWAYRHGITCLNAPGTIDSDYRGEIKVILIN
                     LSDKPFVIENGDRIAQLVIAPVVHANFSISPQLDDTTRGADGFGSTGR"
     gene            9808..11136
                     /locus_tag="PLS88_06570"
     CDS             9808..11136
                     /locus_tag="PLS88_06570"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOU07041.1"
                     /translation="MKERPSFTLWRMTISEIITSFLGTFLFFFIVFLINQVLLFAEDI
                     LSRGADLLSVAKLLFYSLPTILAITIPFSILAATLMTSSRQNADNEYLASSSLGIRPL
                     WLYVPFLIIGLAVSIGSFCLNEWSIPRAAQGFKNEYAELIQKSAKVELAPYSISKYGE
                     KLIVTGGAIEGSSASRLQDVLIIDQSGGYDSDTVIADDVGIEFSKDSLMAILNMNNFT
                     ELKKLNAGSAGDFSVTKAGAAEIRIRIREPLINYSSTAPSEMSLLMLANKIKEKERML
                     DDRIKEKASQEATTLDRLRLNYDLIAIETQQKESTNKNGSLQLLQNDLTSLVNKMRSL
                     KNQKISDASLQVYRLEYEKKLVLPSACFFFALLALPLGIGSKRAGRAAGFGIALLLSV
                     IYWALLFLGQTLGYRQNTNPVISMWMPNFVMLLATCSLWIVRKIKTGHFL"
     gene            11232..12278
                     /locus_tag="PLS88_06575"
     CDS             11232..12278
                     /locus_tag="PLS88_06575"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOU07042.1"
                     /translation="MKKVFIWTIGGTLFLSLVVSLAELFSLLWKFLAQNALFWDIVFW
                     IGLGITNHMVDTLPVAFLFAIVFVLGDWHANHELEAVFSAGISLQRLLIPILAISMIL
                     CAAEFCFTNYVSIPFSRTRNDLQSRMLKESAGKQTVPGLIVDGGKQVYTYRYFDEKNL
                     RLYDVSVIERDTDGNLTRKISAVNAKWEQGAWVFANPTIYKRQDNDWLFLKFENFSDP
                     MIREPPSSFTRPSMNPRLLSMKELNKHIEFLKTSGLPIVDAEVEKQKRLSFSLTPLIV
                     VGLAAAFSGRFKKSVFLLSLLFSLCSATLYYVAQMIASVSAKSGLVSPTISIWAVIIV
                     FSAISAISYLSART"
     gene            12319..13464
                     /gene="tgt"
                     /locus_tag="PLS88_06580"
     CDS             12319..13464
                     /gene="tgt"
                     /locus_tag="PLS88_06580"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOU07043.1"
                     /translation="MIESIFQEMHRDPSCSARTGLLSLPHGNVQTPAFMPVGTNATVK
                     AVEPRDLESMGFSLILANTYHLFLRPGPEVISKAGGLHGFSGWQKNFLTDSGGFQIFS
                     LPQFRKILDEGVRFKSHIDGSSHILTPENVVDIQTAFNSDIQMALDICAPWGESENKA
                     YRATMLTYDWAKRAKTQWLSQRESGYQGYLFGIVQGNFFRELRKMSVEQIASLDLPGI
                     AIGGLSIGEPKEAYIEYLNFTAALLPSDKPRYLMGIGTPDYIIEAVRNGIDIFDCVYP
                     TRTARNGLLFTSKGQITIKKAMYKKDFEPIDPDCSCRVCKTYTRAYLHHLFRNNEILY
                     SMLASQHNLHFMADFASKIRQAIQADSFESFARGFLDTYNAGMEAQE"
     gene            complement(13532..>13960)
                     /gene="greA"
                     /locus_tag="PLS88_06585"
     CDS             complement(13532..>13960)
                     /gene="greA"
                     /locus_tag="PLS88_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOU07044.1"
                     /translation="LGHIMEVDVPANQKEIAYALSLGDLRENAEYKAAKERQDELNSK
                     VGKLKSELDRAQIIDSDQIDSSSISFGTKILLKNLITGENESFTILGPWESDPDHQII
                     SYLSPLGKHLLNHKTGEKLSFTINERKFSYEVLKVEKADF"
BASE COUNT         4396 a         2684 c         3128 g         3752 t
ORIGIN      
        1 aacttcaaga agagatcgat gcaacactaa aagcaggagg gctcgatcta ttggaattct
       61 cattagttcg acacaagggt ggcgatcttc atgtcaaagc ggtaatctat agacccgagg
      121 gaacaggcac cgatgaatgc actaaagcat atcgccttat actgccacgt attcagatgg
      181 cattgggagt gcaagatcca tacattgagg tctatagccc aggtatagac agggctattc
      241 gaacagaaag ggaatgggca gcctttaaaa attgttatat aaaattcatg acttccgaaa
      301 gtaatgactg gaagaaaggt cgcatagtac attttgataa caaaaaagta caaatagaaa
      361 tggacgatac gatattgaat attcccatag cttcgattat aaaagcgaag cttgattcat
      421 ctcgcgaggg agaaaaagta catggcatct gaaatggcag aagcaattca tcagttgatc
      481 tctgaaaagg ggattcctga ggatcttata gttaaaacgc tcgaagaggc gattctagca
      541 gcctacaaga aaaaattcgg aaccaacgat aatgctatag tccgattgaa ggaaaactat
      601 gaaggaatcg aaatctatgc aaaaaagacc atagtctcgg atgaagattt tgaagacgaa
      661 gtcttggaga ttccacttga ggaagctaaa aagcttgcag atgaagctga gatcggagat
      721 attctcgaga ttcccattga tccatccgag ttcgactttc aatcggttat gcttgcaaag
      781 cagacggctc gacagagctt aagagatata tctcgcgata cgctatatgc agagtttaaa
      841 tcgaaagtag gagaaatagt tattgggtat ttccagcgag agcgaaatgg gacgatattc
      901 gttgatctag gcaagattga aggtattttc ccgcgcaagt atcagtctcc gagagagaca
      961 tatcatgtgg gagatcgaat aaaggccctg attgttgaag tggaaaagac caagactgga
     1021 ttccaggttg tgctttcgag gacccatgca gaatttgtgc gcaaattgct tgaactagag
     1081 atacccgaga tctacgacaa gaccattgaa atattcaaaa ttgttcgtga gcccggttat
     1141 aggacaaaag tagcagtata tgcaaagcga agcgatgtcg atccggtagg tgcttgtgta
     1201 ggtccaaagg gcatgcgaag tcaactcatc agccaagaac ttgaaggcga aaagatcgac
     1261 ttcattcgat atgatatcaa cccaaaagag tttatcaaaa atgctcttgc acccgcacag
     1321 gtaaaagaag tatatatact ggacgaagcc agacatatgg cgctagcagt agtcgatgaa
     1381 catgagcttt ccatcgcaat tggaaagtca ggtcagaatg tcaagctagc aaatcggctt
     1441 tgcgactgga atattgatgt tatgacagag gaacagtact tgcaagactc tagaaatgtt
     1501 gaattgaaaa aggccgcaga tcgtctattt tctgtagaag aagaagaggg agaagaattc
     1561 ctgatcagag atttgcctaa tctagagcca gcttggataa atgctcttga atcagcagga
     1621 attcgcttaa ttgaacagtt cctagaattg gatgaggaag agattgcaca gtgcacaact
     1681 ctcagcgctc gagatattag cgcaattcgc gacataatcg aagaaaatgt agaaatcata
     1741 caagaagagg tagaagaaga aggaatcgtg tcttatagtt gtccagaatg tgggtcacca
     1801 ataactatag acatggatca ttgcccatcc tgtggagtag ctctgagttt tgaagcagaa
     1861 gaagagcagg atgaataaag gaaagatatg acagagagca acgataaaga ccttaagaaa
     1921 gtgcatctaa tcaaacagcc tagacaagag actcccgagc agcctcagga attgccagct
     1981 cagaatcctc tcgatcaggg cgcagaaaag aagaaagttg tcgtagtagt aaagaaaaaa
     2041 atagttacca caaagaaagt ccaggcaaaa gcaatgatac cagaagctca tgaagatcaa
     2101 aaaatgagag aaaaacaatc cacattgtca gctcagccac tttccgaagc aggaaagact
     2161 acaacgcaaa taagaacaga ggataaagct gtttctcctt cttcgacagc gccctcgaca
     2221 acaacacttt catcatcaaa atctgcgtca acagctccat ctcagaagtc gttcgaacac
     2281 aagcaggaaa taagacaaga tgagacaatt atcagctctc agcggcgcta ctcttctgat
     2341 ggaagagctg gaaatttggc tcaaggaaga cccagttcta cgtatcggaa tggtcatgag
     2401 agaggagaat ctcgtccagc aagcggatct taccaaggtc gagcacatgg tcaaccatct
     2461 ggaaggagct atccatcgac atctagccga tctggacaat tcggatctca acaatttcct
     2521 catggcagcc gtccttctgg tggttctcaa aacctaagtg ggacaggtcc tagtggtagg
     2581 gcagcctatc cttatgcaaa aacagggcag cctgggggtg cacaaagatc cggtggatat
     2641 actggagcaa gacagagccc tgcaggaggt agatacgggt tgcgtcagac accagcccct
     2701 attccttcca ctgagaacga gagacctgct tctcgtagag caccagctaa aaagaaggga
     2761 gtattcaata agcgcgagga agaaacagaa ctcgagaagc agattcaact caagaaaaag
     2821 gctgaagcaa aattggcggc agttccaaaa tcaattgaca tcatggagaa tatcagcatt
     2881 tcagagttag caaaaaaaat gaatctaaaa cctgctgacc taataagtaa attgatgtca
     2941 cttggcgtta tggcaactat caatcaaaaa atagatgccg atacagctgc gattttggca
     3001 gctgaatata gctgcgaagt aaaagtagta agcctctatg atgagacggt tatcgaaaaa
     3061 gcagcagata aacctgagga acttaagcca cggcctccaa tcgttaccgt aatggggcat
     3121 gtcgaccatg gaaaaactaa acttctagat gcgattcgca aaacagatgt agtgtctcag
     3181 gaatttgggg gaatcactca acacatcggt gcttatatgg tcgaagctcc taatgggaaa
     3241 atatcttttc tggatactcc tggtcatgct gcattcacaa aaatgcgagc acgaggggca
     3301 aatatcacgg atattgtagt tctggtagtt tcagctacag agggtgtcat gacgcagacc
     3361 aaggaagcta tagatcatgc gaaagctgca aatgtcccaa taattgtggc tatcaacaaa
     3421 atcgatctgc ctgaggccaa tcccgacaga gtaaaaacac aactttcaga actaggcctt
     3481 attccagaag agtggggagg ccaaacacaa ttcgtcgaag tatcggctct ccaaaaaatt
     3541 ggcattcctg agctttttga tgcaattctc atacaagctg aaatgctcga cttgaaggcg
     3601 aactacgata ggctagctga tggtaaaatc atcgagtctc ttatagacca gggccgaggt
     3661 attgtcgcta caatcatggt gcaaaacgga accttacata tcggagaccc ttttgttgct
     3721 ggcattttcc caggtagagt aagagctatg ttcgatgata aaggaaatcg tatcgaacta
     3781 gctacaccat ctatgccagt agaagtcatc ggattcgaag gtatgcctga agctggcgat
     3841 ccgttcgaag tggtggaaga cgaaaaattt gcacgccaaa tctcagcaaa gcgccaggaa
     3901 ctgaagaaat acgaagaagg ccgcaatgtt aaaaaggtga ctttagataa cctttacgag
     3961 acgataagcc aaggagagat caaagagcta aaggtcataa taaagggaga tgttcatggt
     4021 agcgtagaag ctctgaaagg catgttagag aagctgtcaa caagcgaagt gcatttaaat
     4081 gttattaggg ctgcagcagg tgctattaca gaagatgatg tcatgatggc ttcagcatct
     4141 aatgctatca ttgttggctt taatgtccgc ccaacatcaa gtgcaaagtt gctagcagag
     4201 cgcgaaaaag tcgatattcg caagtacaat ctcatatatc gtgctcaaga agagataaaa
     4261 cgtgcaatgg aaggcttgct cgctcccgaa ctcaaagaac aagaagtcgg aaaggctgaa
     4321 gtccgcaata tctttagagt gccaaaagca ggcatcgttg cgggttgctt tgtatcagag
     4381 ggtatagtca agcgaaattg tcaagttaga gttatccgag acaacatcga gatattccaa
     4441 ggtaatttat cctcacttaa gcgattcaag gatgatgtta aagaagtagc ggcaggttac
     4501 gaatgcggta tcggtataga gaactgcaat gatctcaaag aaggcgatat ccttgaattc
     4561 tatgagacaa ttgaggttgc tcgaacgctc gagtcgggag acaaaaatga accaggtacg
     4621 caagcgtcgt cttgaagaat tgattcgcga ggaaatttct aagatgatca cctttggtga
     4681 gataaaagat cctcgtgtca acagcttctt atcgatcaca agggttgagg ccagtcaaga
     4741 tggatcgcac gcaaaggtat ttgtaagtag tctcgatgat atagaagggc atctggatga
     4801 ggcgatcatt ggcctatctc atgcagctgg ttttgtacaa tctatcattg caaaacgtgt
     4861 tcgcttgcga ctgacccctg ttctcacctt tgtagccgat cgcagcctga aagaaggatt
     4921 cgagcttacg gaaaaattga aggatcttct gaaataatgc agcatagcgg ttttttcctc
     4981 gtggataaac ctgaaggccc tacttcgaat agagtattgc aggatattcg aaaaagctta
     5041 tcggttgagt tttttgcctc tcaacagaag tttggccatg ctggtactct agattccttt
     5101 gcttcaggtt tattgataat actcacaggc aaggcaacaa ggctgactag atggttcatg
     5161 aatcaaagta aggaatatga tgcagttttc aggtttggag aggagacaga caccctcgat
     5221 cctcaaggca gaatagtaga cataggtgca atacctacaa aagaaactct tgagaatatt
     5281 attccttctt tttgtggaaa tataaaacaa atacctccat cctacagtgc tgtccatgta
     5341 tgtgggaaaa ggtcatatga aatagctaga gctggtaaaa aacccgaaat atctccaaga
     5401 ttcgttacaa ttgataccat agagctgcaa agtttcaagg aaaaagaagc gactcttaga
     5461 attcgttgtt cttctggcac ttatattcgt tctcttgcaa gagacattgg acatgcgtgc
     5521 ggctcttgtg caacagtggt atcgttgaga cgaacaaaaa ttggaccttt ctttataagc
     5581 caagcacatc atccccatga gtgcaatgag aagacattaa aagtcttctc gccagatatc
     5641 gcacagagtc taggtctggc aacagcagta atccaattgg attttgttaa gcattttatt
     5701 catggaagag cattgccaat caaagctata acattcaatg atgatcagat gacattatta
     5761 agaaatctag atttatcctt cgaatcaaag caagtggcac tgttttctga aaaaggtgag
     5821 ctccttggca tagcagagca taaagataaa gagttggtgc caaaggtggt aatggaagag
     5881 gagtctcgat tgtgaatgtt ctgacctggg aagaatttca aaataattat tctcaagaac
     5941 agcttgctgc tacagttggt gtattcgatg gactacatta tggacaccag gttctcatcg
     6001 agactgtaaa gcaacagagc ccagccttaa ggtcatgtgt catcactttt agggagaacc
     6061 ccaaaaaaat acttcatcca tccagctatc agggtagcct cttgactcta gaacaaaagc
     6121 tttccggctt tcagaatatg ggcattgaat attgcgtgat gattgacttt tctaataatt
     6181 tcgctaaact cacgggaaga gaattccttg ctgcactgta tattgcaaat gtacgatttt
     6241 tagcagtagg tgtgaatttt cagtttgggc acaatctcga tactaatgcc acgagggtcg
     6301 agaagctgag tgaagagatt ggcttgcgtt catgtattgt aaaaaatatc atgtatcata
     6361 gccatgctat aagctctagc agaattcgtc atgctgtgct cgaaggaagg cttggagaag
     6421 cggcagaaat gcttggcaga ccatatcaaa tacttgccag gcgggagaga tatgctatag
     6481 ccgaaaaaag tgaatacttt ttacccgaag atgatttgct cataccccct gaagggcagt
     6541 atgaagcgtt cataaaggat aatgatacga ttcaagaaac taaagtgaac gttgttcaca
     6601 atcatctagc attgatgaga ttacgtatgc aatcggaaaa gatccgattg gcgttcgttg
     6661 ataaggctgt tcaggagaag gagaaaatag tatggctttg agcaaagaag acaaggccca
     6721 gttggtgctc gagtatggca aagatgcaaa aaatacaggt gcgattgaga ctcagattgc
     6781 aattatatca gctagaatcg catacctgac cgagcatttt aagactcata agaaagacac
     6841 gaattcaaga cgagggcttc tcaagcttgt cggtcagcgc cgcaagctcc tgaaatacct
     6901 gcagagaacc gatctcgaat cttatcgtgc aattatggag aaattgcaga tccgaaaata
     6961 acaatacaat caaagcgata ttgattagtc caagttaaat cggatatgca aatctagaga
     7021 aggaatgtaa tgataaacaa acttgaatgt aaaattggca acgagattct tgtatttgaa
     7081 acaggaagaa tggcaaagca agcaaatggt gctgtttttg caacctatgg cggtagtgcc
     7141 gttatagcaa ctatttgctg ttcgcctaca gctatagaag gtttggattt tgttccgcta
     7201 actgttgaat attcagagaa atattatgca gcaggcaaga tcccaggtgg tttcataaag
     7261 agagaaacaa agcctaaaga tagggaaatt cttgtttcca gaataatcga tcggcctatg
     7321 aggccattat ttcacaagga atttggacga gaaatccaga ttgttccaac ttgtatctca
     7381 gctgaccaga taaatccgcc tgacattata gcagcaaatg ctgcaagcgc tgcagttcat
     7441 atctccgaca taccttttga tgggcctgta ggatgtgtac gtgtggcact tgtcgaagat
     7501 tcttatattt tgaatcctac ttatgaacag atcgagcagg ccaagctaga aatagtagtt
     7561 gccggtacag cacaaggtat cactatggta gaaggtggct ctcgtgaagc ttcggaagaa
     7621 gagatgattc aggccattga aatggcaaaa gaacctattt cgcagatctg ctctacaatc
     7681 gaagaactaa agcgcatcgc tggaaaagaa aaactcgcgc tagccccact ttccatagag
     7741 ttgaacaaaa aagatgaaat tcgcgaatgc gcctatgaat tgctatcaaa agctcttttc
     7801 acgaaaataa agcaagagcg cgctaaagct gtatcagaag caatttctgc tgtcaaagaa
     7861 aaattcgcag atgatcttaa tgatgaaatt cagatgaagc ttttttcaaa attgatggat
     7921 gatctccagt atgagatcct gcgatcctct atacttgaga aaggattgcg tgtcgatggt
     7981 cgcggtctgg aggacattag gccaataact tgcgaaatcg gcatattgcc acgaacccat
     8041 ggttctgcac tgtttacgcg tggagaaact caagtgctcg ccgtgactac tcttggtaca
     8101 gtattcgatg agcagatatt cgatgatatc gaaggagatc gacgcgagcg atttatgctg
     8161 cactataatt ttccaccctt ctctgtggga gaagttggaa gacttggcac cggaagacgc
     8221 gaaattggac atggtgatct tgctcgccga tcgatcgaac cgatgttacc gccaaaggag
     8281 agatttccat atactatacg agttgtagca gaagtgctcg aatccaatgg ttcttcctct
     8341 atggctacag tgtgctcttc atcgatgtcg cttatgcatg ctggtgtgcc atgcagcaaa
     8401 gctgttgcag gtatagccat gggcctaatt accgatgaaa cacgctatgc tatactttct
     8461 gatattctcg gcgatgaaga tcatcttggc gatatggatt ttaaagtagc gggcacgaaa
     8521 gatggtatca cgggttttca aatggatatc aaaatatcca gtgtttcgac ggagattctt
     8581 cgaaaagctc tagagcaagc aaagcagggt cgccttcata tcctctctat tatggaaaag
     8641 actattgctg aaccacagag cgaaatctca ccctttgctc ctcaggtgat aaatgcgcga
     8701 atcgatcccg aaaaaatagg ccttgtgatc ggcccttcag gcaagaatat aaaggcactc
     8761 tctgaaaaat tcgatgttca gattaacatc gatgaggatg gatatcttac agtttacggt
     8821 aaagaccaga aatccgccta tagtgctcgc gatgctattt tgggaatggt tgaggaacca
     8881 gaagtcggaa agatatatac aggtactgtt aagcggatca tggattttgg cgcttttatc
     8941 gaaatactac cgggtcgcga aggcttatgc catatttcga agctctcaaa gggacatgta
     9001 gacaaggtta gcgatctact taaagaagga gacactatca aggtcaagct catggaaatt
     9061 gatcaccttg gaaggctcaa tcttgcagct gtggatgcgc ttgatgacaa aggacagata
     9121 gcgcagcgag atacgagacg aaatgaaaga ccttctgatc gacagagaga tatgcggtca
     9181 tctcgccatg attcacattg ggataggtaa tcatcgaata atatgatatg tttgaaaatt
     9241 ggtctatttc ttctttgaaa gctatcacta taaacgagat attctatgat atagacctaa
     9301 tttttatagc aataagggta ttctagaatg atgaaacctg aagaaagagc aataggagtt
     9361 ataattgcgc tctccgaagg agcacagatg cctcaatacg ctacagatca tgcagcagga
     9421 gcagatctct atgctgttct agaaaatccc ttagtgctgg aacctttatc gagagcattg
     9481 atccccacgg gtcttagaat tgctctgcct ttaggatatg aggctcaaat caggcctaga
     9541 tcaggatggg cgtatcggca tggtatcacc tgcctaaacg ctcctggaac tattgatagc
     9601 gattatcggg gagaaatcaa ggtaattctc atcaacctga gtgacaaacc atttgttatc
     9661 gagaatggtg atcgaattgc acagctggtc attgctcctg tagttcatgc caatttttcg
     9721 atctcccctc agctcgatga tactactcgt ggagccgacg gttttggatc gacaggccgt
     9781 tgagataaga ccgggataca agttgagatg aaagaacggc cctcattcac actctggcgg
     9841 atgacaattt cagagataat cacttcgttt ttgggtacat ttctattttt ctttattgta
     9901 ttccttatca atcaggtgct gctctttgct gaggacattt tatcaagggg cgccgatctc
     9961 ttatcggtag ccaagttgct tttctattcc ttacctacca tactcgcaat cactatccct
    10021 ttttccatat tagcagctac gctaatgaca tcgtcgcgac aaaatgccga taacgaatac
    10081 ctagcaagct catctttggg gataaggcct ctttggttat atgtgccttt tcttataatt
    10141 ggattagctg tttctattgg atctttttgc ctcaatgaat ggtctatacc tcgcgctgct
    10201 caaggcttca agaatgaata tgcagaactt attcagaaat ctgctaaagt tgaactggct
    10261 ccctattcga tcagtaaata tggagaaaag ctgattgtca ctggtggagc aatagagggt
    10321 tcatccgcaa gccgattaca ggatgtactt attatcgatc agtccggagg gtacgattct
    10381 gatactgtca tagcggatga tgtggggata gaattctcta aggattccct tatggctatt
    10441 ctaaatatga acaattttac agaactaaaa aaactcaatg ctggttctgc aggagatttt
    10501 tcagtgacta aggcgggagc tgcagaaatt cgaattagga ttcgcgaacc tttaattaac
    10561 tatagtagca cagctccttc tgagatgtct ttgctgatgc tagcaaacaa aataaaagaa
    10621 aaagaacgga tgcttgatga tcggattaaa gaaaaggctt cccaagaagc cactacactc
    10681 gatagattaa ggctgaatta tgatttgata gcaattgaga ctcagcaaaa agaatcaacg
    10741 aataaaaacg gctcattgca attgctacaa aatgatctaa catctcttgt caataagatg
    10801 aggtcgctta aaaatcaaaa aattagcgat gcatcacttc aggtctatcg cctcgaatat
    10861 gagaaaaaat tagtccttcc ttctgcttgc ttcttctttg cgcttttagc actcccatta
    10921 ggaattggat caaagagagc agggcgtgcg gctggttttg gtattgccct attgctttcg
    10981 gtaatttact gggcactact attcttaggt cagaccctag gttatcgcca gaatactaat
    11041 cctgtaattt cgatgtggat gccaaatttt gttatgttgc tcgctacctg ttctttatgg
    11101 atagtcagaa agataaaaac tgggcatttc ctatgatgcc gaaaaagaaa acagagcaaa
    11161 gcatagctca acagaaatta gcaaatcggc aagcgactca atttttaccg ataattctgt
    11221 ggagagcctt gttgaaaaaa gtattcatct ggacaattgg aggaacttta tttctttcgc
    11281 ttgtggtatc tcttgcagaa ctcttttctc ttttatggaa atttcttgct cagaatgccc
    11341 ttttttggga tattgtcttt tggattggcc tcggaattac taatcatatg gtcgatacct
    11401 tgccagtagc ttttcttttt gcaatagtat tcgttttagg agattggcat gcaaaccatg
    11461 aacttgaggc tgtttttagt gcaggaatat cactgcaacg cttgttgata cctatcttag
    11521 ctatttcgat gatattatgc gctgctgagt tttgctttac aaattatgta tctattcctt
    11581 tttcaagaac cagaaacgat ctgcagtcga ggatgctcaa agagtccgca ggcaaacaaa
    11641 cggttcctgg acttatcgtt gatggaggaa aacaagtcta tacttataga tattttgatg
    11701 aaaaaaatct tcgtctatat gatgtatcag taatagaaag agacactgat ggaaatctga
    11761 caagaaagat ctctgcagta aatgcaaaat gggagcaagg ggcgtgggtt tttgccaatc
    11821 caaccattta taaaagacaa gataatgatt ggctattttt aaaatttgag aatttttcgg
    11881 atccaatgat aagagaacca ccttcaagct tcacaagacc tagtatgaat cctcggcttt
    11941 tgagcatgaa agagctaaat aaacatatag aattcctaaa aacttctgga cttcccattg
    12001 tagatgcaga ggttgagaag cagaaaagat tatctttttc cttgactcct ttgattgttg
    12061 ttggccttgc cgctgctttc tcaggtcgtt tcaagaaaag tgtttttcta ttgagcttgc
    12121 tttttagctt atgtagcgca actctatact atgtagcaca gatgatagct tctgtatctg
    12181 ctaaatcagg cttggttagc ccgacaatat ctatatgggc tgtaattatt gtttttagtg
    12241 ctatatcagc aatatcttat ttgagcgcta gaacttaaac atgtattata ttgtattctt
    12301 attgaggtaa gtccatagat gattgaatcg atttttcaag agatgcacag agatccttca
    12361 tgctctgcga gaactggtct actttctctt ccacatggaa atgtacaaac tcctgcattt
    12421 atgcctgtag gaacaaatgc tacagtaaaa gctgtagaac caagggattt agagtctatg
    12481 ggattttcct tgattctagc taatacatac catcttttcc taaggccagg gccagaggtt
    12541 atatccaaag caggcggttt gcacggcttc tctggctggc aaaaaaattt ccttacagat
    12601 tccggaggat ttcagatatt ttcgcttcct cagttcagaa aaatactaga tgaaggagta
    12661 cgttttaaat cgcatatcga tggctcttct catattttaa ctccagagaa tgtggttgac
    12721 attcagactg catttaattc cgatattcaa atggcattag atatttgtgc gccttggggt
    12781 gaaagcgaaa acaaagcata tagagcaacc atgttgacct atgattgggc aaagcgagca
    12841 aaaactcaat ggctttctca gcgagaaagt ggttaccaag gatatctttt tggtatcgta
    12901 cagggcaatt tttttagaga acttagaaaa atgagtgtgg aacaaattgc ttctttggac
    12961 ttgccaggaa tcgctatagg aggactttct ataggagaac caaaagaagc atatattgaa
    13021 taccttaact ttaccgcagc tcttttgcca tcagataaac cccgatatct aatgggcata
    13081 ggaacaccag attacatcat agaggcagta cgaaatggta tagacatatt cgactgcgtt
    13141 tatcctacca gaacagccag aaatggctta ttattcactt ccaagggcca aattacaatt
    13201 aaaaaagcta tgtataaaaa agatttcgag cctatagatc ctgattgtag ttgcagagta
    13261 tgcaagactt atacacgtgc ctatttacat catcttttca ggaacaatga aatcctttat
    13321 tcaatgcttg caagtcaaca caacttacat tttatggcag attttgcttc aaagattcgc
    13381 caagcgatcc aagcagatag ctttgaatca tttgctagag gatttcttga tacctataat
    13441 gctggcatgg aagcacaaga ataaatgatg taaatcaagc aataacaatc taggccctta
    13501 gtaattctta ctattaactt tcttaaaaac cctagaaatc tgctttttca acctttaaaa
    13561 cctcatagct gaatttacgc tcgttgatgg taaagctcag tttttcgcca gtcttatgat
    13621 tgagcaagtg tttacccaaa ggagaaagat aagaaattat ctggtgatct ggatcagatt
    13681 cccacggtcc gagaattgta aaagattcat tctcgccagt tattaaattc tttagcaaaa
    13741 tttttgtgcc aaaagaaata ctagagctgt ctatctgatc cgaatcgatg atctgggctc
    13801 gatcgagttc gcttttaagt ttgcctactt tagagttgag ttcatcttgc ctttccttag
    13861 ctgctttgta ttctgcattt tctcgcaaat cgcccaaaga gagggcatag gctatttctt
    13921 tctggtttgc aggaacatct acttccatta tatgtccaag
//
LOCUS       DAOSOB010000054        13347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_4655_length_13347_cov_76.412429, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000054 DAOSOB010000000
VERSION     DAOSOB010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 13347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13347
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4655_length_13347_cov_76.412429"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1130
                     /locus_tag="PLS88_06590"
     CDS             195..1130
                     /locus_tag="PLS88_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU07045.1"
                     /translation="MQSVVAAKPASSSLWRRIKRYKSVYILASLSFIYFIIFKYIPIW
                     NAQIAFRDFQALSGVVKSPWVGLKNFSDFVHSYYFWELIRNTVFYSIGKMVFSVPAAV
                     FLAIALYECRVFWLRKTVQTMAYLPHFLSWVIMYAILLAILSPSNGILNDIIKAFGGE
                     SISFMTDTRYFPWIVILSDMWKETGWSAIIFLAALMGIDPTLFEAAAVEGASAWQRVR
                     YITLPAIRPVLVMVILLRLGTVLDAGFNQIFMLYSLPVYSVADIIDTWIYRQGLLEFR
                     FGLATAVGLFKGFFGMILLMFSNKLIKRYTDTGLY"
     gene            1166..2050
                     /locus_tag="PLS88_06595"
     CDS             1166..2050
                     /locus_tag="PLS88_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU07046.1"
                     /translation="MTSAIKLSRSDKFFHFFIDASLFLILAILIIPIWSTLTLSFRPN
                     DFIGSALDGMFLAPWKWSTAAYRALLGHRSFLHSAGNSFRIFFEGVATALFFTVPLAY
                     ALSIKSLPGRKLLYIIVLIPYLFNPGLIPNYLLVTKLGLVDKLAAVYLPGAVSVYNTL
                     IMKSFFEELPEELKESARIDGASELTVLLRIVLPLSKPILLTIGLYYGVHFWNDFFNA
                     MLYINNAKLQPLPILLRNILLSSGMNEYVEVTAFGEAPIYAIKAASVFLAAIPMLVAY
                     PFIQKYFVKGTMLGSIKG"
     gene            2099..3655
                     /locus_tag="PLS88_06600"
     CDS             2099..3655
                     /locus_tag="PLS88_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU07047.1"
                     /translation="MRKYARFSLAVLLVFVVIASAFGQAAKKPVTIDLWYGAAITEAG
                     PPPDDWVFYKIAREQLGIELKLMALPSNENDQDVKILAAAAANQLPDLFMVRRDPWQV
                     LIKQGLVADVDDMYPLMPHRAVNWFDAESRAYTTINGKSYGLAFPGSLQKNEGLVIRK
                     DWLDKLGLKTPTTLDEFFDVCYAFTYKDPDGNGKNDTYGFGAFVELFDQEQYLGRRFD
                     PIMGAFGVAGTWNMTKENFGLNVKKPEFYDAMQFVKKMVDAKVIDPDWPSLKKDDFRA
                     AWKQGKFGIMREQFAALAAAANYAPFDKNFPNGEWAVIPPPKGPKGKSSVGIYDTNYR
                     IYAVSTKAAKAGKKEAIARLLDYMATDEGYKLIGWGVEGVNYSIDKDGNITDQGVPND
                     TKFSSPKGQTVTQLRNMVFYNSDMELISRYPYFKTINGRTMGPLLYLKTMQNMPWTNV
                     TGAGTIKPHPNNADLKRYINQGVQEFVLGKQPLTKENWQAFIVQMDKLGAAEWEKAAR
                     QQMEEAGYLQ"
     gene            3694..5019
                     /locus_tag="PLS88_06605"
     CDS             3694..5019
                     /locus_tag="PLS88_06605"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOU07048.1"
                     /translation="MLTDTTTMYLQTLASAAATSLAKCTPDLVKWNYETGVLLFSLSE
                     ASKQVFCGLLDGSVRERLSALILPDGEIRGYSFEEFNLDQINAGKLLLREWETSSAGK
                     KANDQEKELKARAPGCQSNHGFWATQCVNHSDSLSYRERLEKAIQTLVEQLSGQPRTA
                     AGSYWHKKIYPYQVWLDGLYMFGPFQAHYGVLFDRADMIDDLCQQFLAVRDKLRHKET
                     GLYFHAMDDSRASRWADPQTGCSPHVWARAVGWLAMALVDALDWIPVAHSKRPYIEEM
                     LVDLLDALMRAQCSSGLWLQVMDAPEAEGNYEETSASAMFAYALYKSLRKDLTADGNS
                     AKRWKNTADSALAGLITKKVRWECASEPSIELPTRESMVELSTGQSTMPRLHLDGICS
                     VAGLGGSPYRDGSIDYYVGEPIVSDDFKGLGPFMLALTEALRLNHIILS"
     gene            complement(5006..6004)
                     /locus_tag="PLS88_06610"
     CDS             complement(5006..6004)
                     /locus_tag="PLS88_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU07049.1"
                     /translation="MNDSAILISIGGVLASAAPIIFAVIGETISERAGVLNLSMNGLI
                     LICAMGSFAVALATGSAFLGFLTGAAIGALVSLIVAFASLTLLQSQVAVGFVLAMLCK
                     DLSYFLGSPIMGKVGPRISQVPIPWLENIPILGPIFFRQDIMTYLSFVLIILASFFIF
                     RTRPGLMLRGIGERPAAAYIRGANVTRLQYLYAVAGGAIAGLAGPIYSLSIKAGWKGT
                     ISGLDGIGWIVLSITIFGGWKPFRAAFGAYLFSFLQWLGLVLQPSLPSVPSQVLQVAP
                     FPLMILTLLFVNIGNAEWVERSLARLPENVRRDIVKVLRAMNATPPAALGEPFRKE"
     gene            complement(6001..7062)
                     /locus_tag="PLS88_06615"
     CDS             complement(6001..7062)
                     /locus_tag="PLS88_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU07050.1"
                     /translation="MPSMRETKASGILFQIASVLFALLVTSLVLILANVEPFAAYKAI
                     ILGAVGSWDLVSNVLVSWVPLLLATSGLLVTFTVGLWNIGIEGQITLGAIFTTWAMRL
                     MQHSGWNPTLILVIAIFAGMFGGMLWAVLAGALKTFGGVNEIFGGLGLNFVATALNIW
                     LIFGPWKRPGVASMSGTLPFDRSLWMPIAAGSSRLTPATIVMAIVGIFVVYVLLENTY
                     FGLKLKAVGKNPRSAYLLGIPTWQHMMASFAICGVFAGLAGAVQVTAVYHRLIPSISS
                     GYGYLGLMVAMLVGYKASLAAPVALFFAALNVGSIQLPIILKLDSSLAGVLQGTLVLC
                     VLLGQGMRAKFLKKKRARL"
     gene            complement(7046..8554)
                     /locus_tag="PLS88_06620"
     CDS             complement(7046..8554)
                     /locus_tag="PLS88_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU07051.1"
                     /translation="MQVEFRGITKCFGTLRANDDISFTVPPSTIQGLLGENGAGKSTL
                     MKILSGYYSSDAGEILLDGKKAHISSPGDAINLGIGMLHQDPLDFPPLKVIDDFILGK
                     KGGILPSRSSAIREFEELADRFDFSIDPEAYVDSLTVGERQQLEILRLLWLGARVLIL
                     DEPTTGISLPQKEKLFATLKKLAGQGMTILFVSHKLEEVELLCDRAIVLRQGKLVGAM
                     DPPFDTKVLVEMMFGKEIELESKAKLSIGQKVLHTKNICLEGARINIKRVELEVRAGE
                     VIGLAGMEGSGQTLFLSACAGLAQPVDGSLCLGGEEKDMTGRSYLEFLKHGVAYLPAA
                     RLEQGLISGLTLMEHFVLAEKMEGFFIDRRKAEELTKDRIALYNIKGGPDSPIESLSG
                     GNQQRALLALLRDPLKLILVEHPTRGLDYESTIYIWSKLKERCARGTAIIFISSDLEE
                     ILTYSDRVLVFFAGKVTPPLDAEKLNVERLGRLIGGKDWENLEKEAAYAVHA"
     gene            complement(8734..9936)
                     /locus_tag="PLS88_06625"
     CDS             complement(8734..9936)
                     /locus_tag="PLS88_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU07052.1"
                     /translation="MRNNHKLFLLVGIALILGGAAFAQGTLPAAGPNLTIGLVMVGPY
                     NDHGWSQANYEGVQYVLQKVPGTKMVYIDRANTADRPGTTVSQLGESLMAQGARLIIF
                     SSDDMTDEAIKFAQDHPDIFVILTSGSQQWKEGKNYLPLPNMINIMGKMEYMKMVAGV
                     AAAMTTKTGKIGYLGPLINDETRRLAASAYLGARYAWVNYLKKDPAKLSFKVTWIGFW
                     FNIPGVTSDPTQVADDFYNSGYDVVISGIDTTEAVAEAVKYNAQGKQVWAIPYDYAKA
                     LDEGKGVSLGVPYFNWGPAYANAVRSAMNGTWKNHFEWNDPDWKNINNPDTSAVGFIK
                     GSLSKDASAAVDKFIDELGKGLNLWVGPLNYQDGTVFLKKGEKATDQQIWYLPQLLQG
                     MEGQSVSK"
     gene            complement(10077..11375)
                     /locus_tag="PLS88_06630"
     CDS             complement(10077..11375)
                     /locus_tag="PLS88_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU07053.1"
                     /translation="MQENTTETVVKGARSTFWIMFFFMLLHQADKLLIGPLTTQIMDS
                     FKISYTQMGAVTTGALIVGAIFYPLWGWLYDRFSRPKLLALASFLWGTTTWLNAIAPN
                     YSTFLITRASTGIDDSSYPGLYSLISDYYPPKKRGKIYGFLQVAQPLGYLIGMVLALV
                     FGASFGWRNVFYLTGSMGIVVSFIVFLFVKDRPRGSTEPELSSVENLERYKFSWKTVG
                     GLFKKKSLIILFLQGFIGVFPWNVITYWFFDYLKTERGYSDMQTLMTMVPAVIILAAG
                     YPAGGALGDKLFKKTPKGRVIVGAAGVAIGAIFLWITMKIPIDAHLPFAIMLFATAFF
                     IPFASPNVLSSFYDVTEPEIRATTNAVQNFVETAGSALAPLMAGIIADRSTLGNAILW
                     ICVGAWAICFAFFIFAGRFIPKDIADLREKLRERAAESAS"
     gene            complement(11463..11849)
                     /gene="queF"
                     /locus_tag="PLS88_06635"
     CDS             complement(11463..11849)
                     /gene="queF"
                     /locus_tag="PLS88_06635"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOU07054.1"
                     /translation="MTDYEFKALGKSTRTPSRELETFPAPASVTRVVLESDEVTSLCP
                     VTGQPDWDMVRIEYEPRDLCIESKSLKLYLWSFREEGVFCEAFAARIAKDVFDAIKPK
                     SVIVTVTQKPRGGITICAESRLPETN"
     gene            complement(11852..12538)
                     /locus_tag="PLS88_06640"
     CDS             complement(11852..12538)
                     /locus_tag="PLS88_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOU07055.1"
                     /translation="MNGKQYTHSTIFLVLTALYITCLLISNIIASKLIQIAGIVLPSA
                     VILFPVTYILADVFTEVYGFRKMRTVIWLGFGANAFMSLIFLVAIALPSPLFFGEQEA
                     YRIVLSNSPRIFLASLAGYWAGEFSNSIILSALKKATKGRLLWTRTIASTIVGEGLDT
                     ILFIGIAFWGTMPKASLIQMMLAQYLFKVAYEVAFTPLTYLVVGYIKRKENIDTFDRD
                     VVYNPFRLGE"
     gene            complement(12652..>13347)
                     /locus_tag="PLS88_06645"
     CDS             complement(12652..>13347)
                     /locus_tag="PLS88_06645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOU07056.1"
                     /translation="CVFGDSIARGVILDDQGSYKPLKDCFASLAAEELGVALVNKARF
                     GCTIAKGREIIERTLDRFLGKSKEPVFDYVFLEFGGNDCDFRWDEISVKPYAQHLPAT
                     PPELFVKQYSEVIEELRARGLKPILLTLPPLVAERYFAWFTRTGLDQEAILKWLGDVE
                     FIYRWHESYSCLVWEIGEKYDAPVIDIRKTFLEQRNYQRFLCKDGIHPNVDGHSLIKA
                     EIVAFAKQHLVES"
BASE COUNT         3531 a         3170 c         3251 g         3395 t
ORIGIN      
        1 gggcttgcag agattgcaga tttgaccggt ttccaggacg aagcatattt ctgcagggtt
       61 tttaagcgaa ttgcgggcac gacgccagga tattttcgtt ctagatcttt tttagatgta
      121 agaaaagtac aagaatctga ctgaaaaaga gcagatttat cgcttatcat ttaaaataaa
      181 gaataaagat ggaaatgcaa tctgtcgtag ccgcaaagcc ggcttcatct tctctatggc
      241 gtcgcataaa gcgatataaa tcggtctata ttcttgcttc tctttcattt atctatttta
      301 tcatcttcaa gtacattccc atatggaacg ctcagattgc ttttagggat tttcaagctc
      361 taagtggagt ggtaaagagt ccctgggttg gattgaagaa tttctccgat tttgtacata
      421 gctattattt ctgggaactt atccgcaata cggtttttta cagtatcgga aagatggtgt
      481 tctctgtgcc tgcggctgtg tttcttgcaa ttgcgctgta cgaatgtagg gttttttggc
      541 ttagaaaaac ggtgcagaca atggcatatc tgccgcactt tttgtcttgg gtcatcatgt
      601 acgccattct tttggcaatt ctgtcgcctt ccaatggaat cttgaatgac atcataaagg
      661 cttttggcgg agagtctatt tcctttatga cggatactag atatttccca tggattgtca
      721 tcctatcgga tatgtggaaa gagacggggt ggagcgcaat cattttcctt gcggctctga
      781 tgggtattga tcctactcta ttcgaagcgg ctgcagtgga aggggcaagc gcttggcaga
      841 gagttcgcta tataacgctg ccagcaatcc gtcctgtcct tgtaatggtg attctcttga
      901 ggcttggaac agtgttagat gcaggcttta accaaatatt catgttgtat tcgcttccgg
      961 tttacagtgt agcggatatc atcgatacct ggatttatcg ccagggcctt ctagagttta
     1021 gatttggact tgcaaccgca gtgggactat tcaagggctt ctttggcatg attctgctta
     1081 tgttctcgaa taagctcata aagcgatata cggacactgg gctttattga agggggatgg
     1141 ttttgatgaa ggagtttggt cttatatgac aagtgcgata aagttgtcaa gaagcgacaa
     1201 gttttttcac ttttttatag atgcatcgct tttcttgata ctcgccattc tcatcatacc
     1261 gatatggagt acattgacac tttcgttcag accaaatgat tttatcggct ctgcgctcga
     1321 tggaatgttt ctggctccct ggaaatggtc gacagcggca tatagggcgc tcttagggca
     1381 taggtctttt cttcattcgg ccgggaacag ttttcgcatt tttttcgagg gtgtagcgac
     1441 tgcgcttttc tttacggttc cactggcgta tgcactttcg ataaagtcgc tgccgggaag
     1501 gaaattgctc tatatcatcg tgctcatccc gtacttattc aatccggggc tcattccgaa
     1561 ctatctactg gtaaccaagc tggggttagt ggacaaattg gccgctgttt atctgccagg
     1621 tgccgtaagt gtctataaca cgctcataat gaaaagcttt tttgaggaac tgccagaaga
     1681 gctaaaggaa tcggcacgca tagacggggc ttcggagctt acggtgcttc taaggattgt
     1741 cttgcccctt tctaagccaa ttctgctcac aattggcttg tactatggag tccacttctg
     1801 gaacgatttt ttcaacgcaa tgctctatat caacaatgca aagctccagc cgctaccaat
     1861 tttattgcgg aatattttgc tttcttcggg tatgaatgaa tatgtggagg tcactgcctt
     1921 cggagaagct ccaatatatg ccattaaggc agccagtgta ttcttagcgg caattccaat
     1981 gttagtcgca tacccattca ttcaaaagta ctttgttaag ggtaccatgc taggaagcat
     2041 caaaggataa tagcaaataa taattgaagg gcggcatttg gccgcaaagg aggttcgtat
     2101 gagaaaatat gcaaggttca gcttggccgt tcttctcgta tttgttgtca ttgcatcggc
     2161 atttggtcag gctgcaaaga aaccggttac gatcgatctg tggtatggcg cagccattac
     2221 tgaggccggt ccgccaccgg acgactgggt attctataag atcgcccgcg agcagttggg
     2281 catcgaacta aagcttatgg ctcttccgtc gaacgaaaac gatcaggatg taaagatcct
     2341 tgccgcagcc gcagctaacc aattgcccga tcttttcatg gttcgccgtg atccatggca
     2401 agttcttatc aagcaaggac ttgttgccga tgtcgatgat atgtatcccc tcatgcccca
     2461 ccgtgcagta aactggttcg acgcagagag tagggcatac actacgataa atggcaagag
     2521 ctatggtcta gctttccctg gttcgctcca gaaaaacgaa gggcttgtga ttcgcaagga
     2581 ttggttagat aagcttgggc tcaaaacgcc gactacattg gatgaattct tcgatgtctg
     2641 ctatgccttt acctataagg atccggacgg aaatggcaag aacgacacct atggttttgg
     2701 agccttcgtc gagctcttcg atcaggaaca atatcttgga cgccgcttcg atccaataat
     2761 gggagccttt ggcgttgccg gaacctggaa catgacaaaa gagaattttg ggctcaatgt
     2821 aaaaaaacct gagttctacg atgccatgca atttgtcaag aaaatggtcg atgcaaaggt
     2881 tatagatcca gactggccga gcttgaagaa ggatgacttc cgtgcagcgt ggaaacaagg
     2941 caagttcgga attatgcgcg agcaatttgc ggcgctggca gcagcagcta actatgcacc
     3001 cttcgacaag aatttcccga atggtgagtg ggctgtaatt cctccgccca agggtccgaa
     3061 aggaaagagc tctgtaggaa tctacgatac gaattaccgc atctatgcag tttcaacaaa
     3121 agcagccaag gcaggcaaga aggaggccat cgcaaggctt ctcgactaca tggcgaccga
     3181 tgaggggtac aagcttatag gctggggtgt ggaaggagtc aattactcga tcgacaaaga
     3241 cggcaacatt accgatcaag gcgtaccaaa cgataccaag ttttcctccc caaaaggtca
     3301 gaccgtcact cagcttcgca acatggtctt ctataacagc gatatggagc ttatctccag
     3361 atatccatac ttcaagacca tcaatggccg caccatgggc ccattgctct acctcaagac
     3421 aatgcagaac atgccatgga ccaatgtgac aggcgcagga acaataaagc cgcatcccaa
     3481 caatgccgat ctgaagcgct atatcaatca gggggtccag gagtttgttc ttggcaagca
     3541 accgctcacg aaagagaact ggcaggcctt tattgtccag atggacaagc ttggcgcggc
     3601 ggagtgggaa aaggcagctc gtcaacaaat ggaagaagct ggctatctac aatgattagt
     3661 tagctctata gctctaatta gggggtagtc gcgatgctca ccgacaccac aactatgtac
     3721 ctacagactc tagcatcggc agccgcgact tcccttgcta aatgcacacc cgacttggtc
     3781 aagtggaatt acgagacggg tgtgcttttg ttttcgctca gcgaagcctc caaacaagta
     3841 ttctgtgggc tcctagatgg ctctgttcga gaaaggctct ccgcgctgat tcttccggat
     3901 ggcgaaattc ggggatactc ctttgaagaa ttcaatctag accagataaa tgcaggaaag
     3961 ctacttctcc gggaatggga gaccagcagc gcagggaaaa aggccaatga ccaagagaaa
     4021 gaacttaaag ccagggctcc gggttgccaa agtaatcatg gcttctgggc cacccaatgc
     4081 gtaaaccata gcgatagtct gtcttatcgc gaacgactag aaaaagccat ccagactctc
     4141 gtggaacagc tatctgggca gccccgtact gctgccggtt catattggca taaaaagata
     4201 tatccgtatc aagtatggct cgatggcctt tatatgtttg ggccttttca ggctcattat
     4261 ggggttctct tcgatcgagc agatatgata gatgacctgt gtcaacaatt tttggctgtt
     4321 cgagataaat tgagacataa ggaaaccgga ctctatttcc atgcaatgga cgatagtaga
     4381 gcgtctcgct gggcagatcc gcagacaggt tgttcacctc atgtatgggc tcgagcagtt
     4441 ggttggcttg caatggcgct tgtggatgcg ctcgactgga ttcctgttgc gcactctaag
     4501 cgaccttaca tcgaagagat gcttgtagat cttctcgacg ctcttatgcg cgcacagtgt
     4561 tcttcgggac tttggctcca agttatggat gctccggagg ccgaaggcaa ctacgaagag
     4621 acatcagcct cggctatgtt cgcttatgca ttatataaga gtctgcgaaa agatcttacc
     4681 gcggatggaa atagcgcgaa gagatggaag aatactgcag actcggcgct agcggggctg
     4741 attaccaaga aagtaagatg ggaatgcgct tctgagcctt cgatagagct tccgacaagg
     4801 gaatcgatgg tagaactatc gacaggccaa tctacaatgc cgcgtttaca tctcgatgga
     4861 atctgcagcg tcgcaggcct tggcggatct ccatacagag atggctcgat tgattattat
     4921 gttggagagc ctatagtgtc ggacgacttc aaaggccttg gtccatttat gcttgcatta
     4981 accgaagccc ttcgcttaaa tcacatcatt ctttcctgaa tggctcgcca agtgcggcag
     5041 gcggggtagc attcatagct ctcaagacct tgactatatc tctgcggaca ttctcgggca
     5101 aacgcgcgag agaacgttcg acccactctg cattgcctat atttacaaag agcagtgtca
     5161 ggatcataag agggaaaggt gccacctgga gcacttggga tggaacggat ggaaggctgg
     5221 gctgcagcac gagccctaac cattgaagga aagaaaagag gtatgctcca aaggctgccc
     5281 taaatggctt ccaaccacca aaaatagtta tggagagcac aatccagccg ataccatcaa
     5341 gacccgaaat agtgcctttc catcctgcct ttatggaaag agagtatata gggcctgcga
     5401 gcccagcaat tgctcctcct gccacggcat atagatattg caggcgagtc acattagcgc
     5461 cgcgaatata agctgcggcg ggtcgctcgc ctattccccg cagcataagt ccaggcctag
     5521 tcctaaaaat aaagaacgac gcaagtataa tcagtacaaa ggataaatag gtcattatat
     5581 cctggcgaaa gaaaattggc ccaagaatcg ggatgttctc tagccaagga attggcactt
     5641 gcgagatacg gggtcccacc tttcccatta ttgggctgcc aaggaaatag gataggtctt
     5701 tgcataacat tgccaacaca aagccaacgg ccacctgtga ctgcaaaagg gtcaggcttg
     5761 caaaagccac tatgagagaa acgagagctc ctatcgctgc tccggtcaaa aagcccaaga
     5821 aagcgctacc cgtcgccaag gccaccgcaa aacttcccat agcgcagata agaatcaatc
     5881 cgttcatgga aagattcagc actccagctc gttcggaaat tgtctcacca atgacagcga
     5941 aaatgatagg cgcagccgaa gcaagcacac cgccaatgct aattagaatc gcagaatcgt
     6001 tcatagtctt gcccttttct tttttagaaa cttagcgcgc ataccctggc caagaaggac
     6061 gcaaaggacg agtgttccct gaagcactcc ggccaacgaa gaatccagct tgaggatgat
     6121 cggcaactga atggagccga cattcaacgc ggcaaaaaac aaggcaactg gagctgccaa
     6181 gcttgcctta tagcccacta acatggctac catgagaccc aaatagccat agcccgacga
     6241 tatagaaggt ataagacggt gatagactgc ggtaacctgc acagctccgg caaggcccgc
     6301 aaaaacgcca cagatggcaa aagacgccat catatgctgc caggttggaa ttccgagcag
     6361 gtaagcgctc cttgggttct ttcccactgc cttgagtttt agaccaaaat aggtattttc
     6421 gagtagcaca tagacaacga agatgccgac tatagccatg acgatggttg caggcgtgag
     6481 cctagaggaa cctgcggcga tcggcatcca taaggagcgg tcgaaaggca gagtgcctga
     6541 catggaagcg acacctggcc gtttccatgg tccgaagatt agccagatat tgagagcggt
     6601 cgccacgaag ttgaggccaa ggcctccgaa aatttcattt acgcctccga aggtcttgag
     6661 tgcccctgca aggacagccc atagcatgcc tccgaacatt cccgcaaaga tagcaataac
     6721 cagaataagc gtaggattcc atccagaatg ctgcataagt cgcatggccc aggtagtaaa
     6781 tatcgctcca agtgttatct gtccttctat cccaatgttc cacaagccca ctgtaaaggt
     6841 tacgagaagc cccgaggttg ccaagagcaa aggcacccaa gagacgagaa cattgctcac
     6901 caagtcccag gaacctactg cgcctagtat tatggcttta taggcagcaa aaggttcgac
     6961 atttgccaga ataagcacta gagatgtaac caagagcgca aataggacag atgcaatctg
     7021 aaatagaatg ccgctggctt tcgtttcacg catggacggc ataagcagct tccttttcta
     7081 gattttccca atccttgcca ccgatgagcc gcccaagacg ctccacattg agcttttccg
     7141 catcgagtgg tggcgtgacc ttgccagcaa agaacacgag aactctatcg ctatatgtga
     7201 ggatttcctc gagatccgaa gaaatgaaga tgatggcggt gccgcgagcg catctctctt
     7261 tgagcttgct ccagatatag attgtcgatt cgtagtccag tcctcgcgtg ggatgctcga
     7321 ccaagatgag cttgagagga tcgcgcagaa gcgcaagaag cgctctctgc tgattacctc
     7381 cagaaagcga ttcgatgggg ctgtccggcc ctccttttat gttgtagagc gcgattcggt
     7441 ccttcgtaag ctcttctgcc tttctgcgat cgatgaagaa accttccatt ttttccgcta
     7501 gcacaaagtg ctccatcaga gtaagaccgc tgataaggcc ttgctccaac cttgcggcag
     7561 gaaggtaagc aactccatgc ttgagaaatt ccagatagga tctacctgtc atatccttct
     7621 cttctccgcc gaggcaaaga gaaccatcga cgggttgagc aagccctgca caagcgctca
     7681 gaaaaagagt ttggccagaa ccctccatgc ctgcaagacc aataacctca ccagctcgaa
     7741 cctctagctc tactctcttt atattgattc tagccccttc taagcatatg ttctttgtgt
     7801 gtagtacctt ttgccctatc gaaagcttgg ccttggattc gagctctatc tctttgccaa
     7861 acatcatttc gacaagcacc ttggtatcga aaggcggatc catggctccg actagcttgc
     7921 cctggcgaag gacaatagcc ctatcgcaga gcagctccac ttcttcgagc ttatgggata
     7981 caaagagaat tgtcatgcct tgtccagcca atttcttcag cgtggcaaag agcttttctt
     8041 tttgtgggag acttatgccg gttgttggtt catctagaat gagcacccga gcgcccaacc
     8101 acaaaagacg caatatctca agctgttggc gctcgcccac ggtaagagaa tctacatagg
     8161 cctctggatc tatcgaaaaa tcaaaacggt ctgcaagctc ttcgaactcc cttatggcgg
     8221 aacttcggct cggcaaaatt cctccttttt tgccgagaat gaaatcgtct atcaccttaa
     8281 gaggcggaaa atccaaaggg tcttgatgta gcatgcctat acctagattt atggcatctc
     8341 caggggaaga gatatgggcc tttttgccat ccaacaaaat ttctccggca tcggagctgt
     8401 aatagcccga aagaatcttc atgagggtgc tcttgccggc tccattctct cctagaaggc
     8461 cttgaattgt tgagggaggg actgtgaatg aaatatcgtc gtttgctcgc aatgtgccaa
     8521 agcactttgt tattccacga aactcgacct gcattgaatc cttcttccga tttatttatc
     8581 ctcgccatct tgaaattgaa gtggtaagtg tcaattcaag tcttaagcat gtcaaaaata
     8641 gaacgcggag gtcggctggc tatgtattag ccaagcctat acctccgaca tactagagaa
     8701 aaagctctct atcaaatgaa atgtagaagc caattatttc gatacggatt gtccttccat
     8761 gccctgcaag agctgtggca ggtaccagat ctgctgatct gtggcttttt ctcccttttt
     8821 caagaatacc gtgccatctt gataattaag cggtccaacc catagattga gtcccttgcc
     8881 tagctcgtcg ataaacttat cgaccgcggc ggaagcgtct ttggagagcg atcccttgat
     8941 aaagcccact gccgaggtat ccggattatt gatgttcttc cagtcaggat cattccattc
     9001 gaagtgattc ttccaggtgc cattcatggc ggatctcacg gcgttggcat aagcgggacc
     9061 ccagttgaaa tacggtacac caaggctgac gcccttgccc tcatcgaggg ccttcgcata
     9121 atcatagggt atagcccata cctgtttgcc ctgtgcatta tattttacag cctcggcaac
     9181 tgcttctgtc gtatctatgc cagagataac cacgtcatat ccggaattgt agaagtcatc
     9241 tgcgacttga gtcggatcgg atgtaacgcc tgggatattg aaccagaatc ctatccaggt
     9301 aaccttaaag ctcaacttgg cgggatcttt cttgagataa tttacccagg catagcgagc
     9361 acctagatag gcagatgccg caagacgcct tgtttcatcg ttgataagag ggccaaggta
     9421 accaattttg ccagtcttgg tcgtcattgc agccgccaca ccggccacca tcttcatata
     9481 ctccattttt cccatgatgt ttatcatgtt gggaagaggc agatagttct tgccttcttt
     9541 ccactgctga gagccagaag ttaagatgac gaagatgtca gggtgatcct gagcgaattt
     9601 gatagcttcg tctgtcatat cgtccgagct gaaaatgata agccttgcgc cttgtgccat
     9661 aaggctttcg ccaagctgag acacggtagt gccaggtctg tctgcggtgt tggctctatc
     9721 tatatagacc atcttggttc caggcacttt ctggagcaca tactgaactc cctcatagtt
     9781 agcctgactc cagccatgat cgttgtaagg accaaccatg actaaaccga tagtcagatt
     9841 cgggcctgct gcgggtagag tgccttgggc aaaggcggct ccacctaata taagagcaat
     9901 accgaccagc aaaaagagct tgtgattgtt acgcatcttt ctactccttc aaattccgtt
     9961 tcggcaaagc cgagcaattc gattttttca tgagctgagg gagatgtcaa gtttatttgg
    10021 ctgataaccg agcaacatcg cctatttctc atgatgcgct ttttgtggcg cacttttcat
    10081 gatgcgcttt ccgcagcgcg ttcccttaat ttttcgcgca gatcggcgat atccttagga
    10141 atgaatcgcc ctgcaaagat gaagaaggca aaacagatag cccatgctcc gacgcatatc
    10201 caaagaatag cattacctag tgtgcttctg tctgcaatga tgcctgccat tagaggcgca
    10261 agggcagatc cggccgtttc gacaaaattc tgaacggcat tggtcgtggc tcgaatttcc
    10321 ggctcggtaa cgtcataaaa ggacgagagc acattgggcg aggcgaaagg aatgaaaaac
    10381 gccgtggcaa aaagcataat ggcaaaaggc agatgagcgt cgataggaat tttcatcgtt
    10441 atccaaagaa atatcgcccc gatagcaaca cccgccgctc cgactataac gcgtcctttg
    10501 ggcgtcttct tgaagagctt gtcgcctagt gcgccacctg cagggtagcc tgccgcaagg
    10561 attattacgg ctggaaccat agtcataagc gtctgcatgt cgctatagcc gcgctctgtc
    10621 tttagataat cgaaaaacca ataggtaatt acattccatg ggaatacgcc tataaatcct
    10681 tgtaaaaata ggattatgag gctctttttt ttaaatagcc cgcccacggt tttccagctg
    10741 aatttatagc gttcgagatt ctcgaccgat gagagttctg gctcagtgga gccacgtggt
    10801 ctatctttta cgaaaaggaa gacgataaaa gaaaccacga tgcccatgga acccgttaaa
    10861 tagaacacat tgcgccagcc gaagctcgca ccgaatacga gagcaagcac catgcctatg
    10921 agataaccga gcggctgagc tacttgcaaa aatccatata ttttcccccg ctttttaggg
    10981 ggataatagt ccgaaataag gctatagagg cctgggtaac tagagtcatc tataccagtg
    11041 cttgcccttg ttatcaagaa agtcgagtag ttgggggcaa ttgcgtttag ccaagtcgtt
    11101 gtcccccaga gaaaagaagc gagagcaaga agctttggcc tcgagaatcg gtcatagagc
    11161 cagccccata aagggtagaa gatggcaccc acaatcagtg cgccagttgt gaccgctccc
    11221 atctgggtgt agctgatttt gaagctgtcc atgatctggg tagtcagcgg ccctattagg
    11281 agcttgtccg cttgatgcag tagcatgaaa aaaaacataa tccagaaagt cgagcgagct
    11341 cccttgacta cagtttcagt cgtattttcc tgcatcgtat gcctcctatg ggcagcatag
    11401 cacaggtatg ggctgggttt tagttcttat ctttcatttt ggtttatttt tggcaaattt
    11461 atctaatttg tttcaggcaa tctcgactct gcacagatcg taataccgcc gcggggtttc
    11521 tgtgtaacag tgactatgac gctctttggt tttattgcat cgaatacatc cttggcgatg
    11581 cgagcggcga aagcctcgca gaatacccct tcttctctga atgaccacag atagagcttg
    11641 aggctcttgg attcgatgca tagatcccgt ggctcatact cgatgcgcac catgtcccaa
    11701 tccggttggc cagtaacggg gcagagactt gtgacttcat cgctctcgag aaccactctg
    11761 gttaccgaag cgggcgcagg gaaagtttcg agctcgcgcg aaggagtgcg agtgctcttg
    11821 ccaagagctt tgaattcata atcggtcatg ttcattctcc taatctgaat ggattgtaga
    11881 cgacatctct atcgaaggtg tcgatattct ctttgcgctt tatgtagcct actactagat
    11941 atgtcaaagg cgtaaaagcg acctcatagg cgaccttgaa aaggtattga gcgagcatca
    12001 tctggattaa gcttgccttg ggcatggtgc cccaaaatgc gataccgatg aaaagaatcg
    12061 tatcgaggcc ctctcctaca atggtcgagg ctatggttct tgtccaaagc agccgcccct
    12121 ttgttgcctt tttgagggcc gagagtatga tcgaattaga gaattctcct gcccaatatc
    12181 cagctagaga agcaagaaaa attcttggcg aattgcttag aacgattcta taagcttcct
    12241 gctcgccgaa gaatagcgga gagggtagcg caatagccac caggaatata agagacatga
    12301 aggcatttgc gccaaagcca agccagatca ccgtgcgcat cttccgaaag ccatacactt
    12361 cggtaaagac atccgcgagg atataggtaa ctggaaatag aatgacggca gaagggagaa
    12421 ctatgcctgc aatctgtatg agtttgctgg cgataatatt agagatgagc aaacaggtaa
    12481 tgtagagcgc cgtaagtacg agaaagatgg tggaatgtgt atattgcttc ccattcatgg
    12541 gaaagcctcc tgttttgttc aggcggggaa ccgcgaccgc cgtgggataa aatgcagtct
    12601 atctcagcgc gatgctttgt gcaacggatg gctagaagaa atgtagctta attagctctc
    12661 cacaagatgc tgctttgcaa acgcgactat ctcggccttg atgagcgagt gaccatccac
    12721 atttggatgg atgccatcct tgcacaagaa tcgctggtaa ttgcgctgtt cgagaaatgt
    12781 cttacgtata tcgattacgg gagcgtcata tttctcgcca atttcccata caagacatga
    12841 atagctctca tgccagcgat agataaattc gacatcgcct agccatttga gtatggcctc
    12901 ttgatcgaga cccgtgcgag tgaaccatgc aaagtagcgc tcggctacga gaggcggaag
    12961 cgttagaagt attggcttta agcctcgggc tctcagttct tctatgacct cgctatattg
    13021 cttgacgaag agctcgggag gagttgcggg aaggtgctgg gcatagggct tgaccgatat
    13081 ttcatcccat cgaaaatcgc agtcattccc tccgaattcg aggaagacgt agtcgaatac
    13141 aggttctttg gatttgccaa gaaaacggtc aagcgtgcgc tcgatgatct cccgaccctt
    13201 tgcaatggta caaccaaaac gcgccttgtt gacaagcgca acacctagct cctctgccgc
    13261 aagcgaagca aagcaatctt tcagtggttt ataagagcct tgatcatcga gtatcacacc
    13321 tcttgcaatc gaatctccaa aaacaca
//
LOCUS       DAOSOB010000055        12761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_4909_length_12761_cov_66.369274, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000055 DAOSOB010000000
VERSION     DAOSOB010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 12761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12761
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4909_length_12761_cov_66.369274"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PLS88_06650"
     CDS             <1..959
                     /locus_tag="PLS88_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU07057.1"
                     /translation="MTALANSAGTVIREVSVKGIIKFNQSNEQLAGITLVDADTMRDL
                     LGFASLRDGAPQLSEHEAAFLTDFDPDSLFDNLFASQTIMTEGEEQPDIASGLSSEDI
                     PENTGLRTAQVSSSTTVTAEIVPAWQFLLVRAKKGANTSKLLDELQVFSEQLGDGDRV
                     QDWVAGAGKVARTAVTIRLVFDLLVAIIAVVVIMIMMNVLVISVNERLHEIGTLRAIG
                     AKRHVVRSMILYETTFMALIAGAAGLILGFILLLIMGKVGIRAPNLFFEALFGGEILK
                     PVISIGAAIRAFLWILAMALAASWYPTRVAVRIEPVVAMRGE"
     gene            962..2260
                     /locus_tag="PLS88_06655"
     CDS             962..2260
                     /locus_tag="PLS88_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU07058.1"
                     /translation="MHLSNLKLHFKLAARNVRRQPNRTIALGGAVAFSALVMTLISGF
                     VSGMDTAIQDNVTLYSGGHILISGYTPSWSGRLQNRFVDDEIATIVGSAVTDIRTVSP
                     LAQARATVVFGTREIQLTLRGIDWTKDELYHDSLLLSSGDWKALNEHDRSMLMSSQTA
                     DRFGLAIGDQVVVRLTTASGQQNVTDYTVSAIYDDAASGVMTTAFVSFEDLIADLNMK
                     PNEQQQIAIFLKDSTNAEHAAKAIATALAEKGYSIASGSEQATQRRMNSGKAVYSIST
                     VQELAGEIGSALGSIRWIGYAVFILMLLVSATGIANSYQMVLLERTKEIGMLRCIGFK
                     KKDVFASFISEGILLAGAAAIAGILCALPIGLGISFIPFDPHGDFGAALVKGHLRFIP
                     TLGQLLIILVFIMIVAVVAVFLPARKAAEVVPVEALRITA"
     gene            2302..3219
                     /locus_tag="PLS88_06660"
     CDS             2302..3219
                     /locus_tag="PLS88_06660"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOU07059.1"
                     /translation="MLRLYEKKTKYFPSKSIRDISLVILGLVLVVCAQAQGSPPPGGP
                     KGGGGPMAGIGLPLGFLEEDKDYKFPDDITKYTAKDFLMLVRRTDIRSSFYNSDMSAT
                     ITTVNVSPTRGTFVTKEQIFRRDKDDAFLMLTLEPESRKGQGMLRVNNNMWRYDPTSR
                     KFTHTTLKDTYENTTVTNNDFRRWQRSIDYSVEKLSTGTLGNYKVIIGELRANNDEVP
                     FPYIKMYVEADRKIVLKVEEYSLSMKLLRTAYYTQYVQIGDSFVPTVQIFQDGLVPEK
                     RSQVTYTNISTKPLPDYYFTKEYLERVSQ"
     gene            3230..4852
                     /locus_tag="PLS88_06665"
     CDS             3230..4852
                     /locus_tag="PLS88_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07060.1"
                     /translation="MKTRIIKSLLFLIMTTLFLFSKSSIWAQSSSSSSSFKPPEVIDE
                     SELFGGPKDIVTVIDPSVASAGTVQLVEDTKTYPVFLISGSAGAGLYGNYTPLGGSSS
                     DKQTLYGAVDISDLEFDYLPAENLHFSLSSNLLAVPNQILAASASAYADLRQSDLVRL
                     YASGSFKYDPSSYLSTNQEYISPTFSLDELFVDTHIEETVFFRLGKQRISWGVGNWFK
                     PSDVLSLSAIDPDDPTAAREGPFAFKVDVPMKLNHLMVYMVPPISDDAASSSIASKYD
                     LVVNGWELSFGAYARADMLARPRAIFSFTGAIGSFDVYGENVLLYGSDRIYAKESSTP
                     GTYEAYRVENEPFFQSTLGVKYSKSYSGGLSYSIHVQGYYNGMGYADSSIMQSAAAKA
                     AIKKAIEDDYPTAYSYSDSSIDAGMWYLAGSASVSGRFGEGNNLTRVTGSVYALANFS
                     DGSLRFNPQLKVQVGDEGGKVSFTVSDLTSIGDVGSEYAKNGNITTPALTVSLLNSEV
                     SLQASVPINLNADYSVKKLQTKFSIFWNAIEF"
     gene            4923..5627
                     /locus_tag="PLS88_06670"
     CDS             4923..5627
                     /locus_tag="PLS88_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU07061.1"
                     /translation="MHGKILIVEDTKELAELYKLYLQNEGFECKLAFDAESALPIAQK
                     EEWDLVVLDLNLPGMDGFQFLEQLRKTKSMPVMILTAREADEDVIFGLGVGADDFVTK
                     PCPPKVLAARIRAHMRRTATLQMRQNGSMVRFGPYELDLEGMYLTKNAEPISLSAREF
                     HILKELVSNPGRPFTPEDLYTRVWGQEYGDISAIGVYIQRLRKKLEDDPANPYYIQTQ
                     YGMGYRFNPEAFVEKA"
     gene            5624..7066
                     /locus_tag="PLS88_06675"
     CDS             5624..7066
                     /locus_tag="PLS88_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU07062.1"
                     /translation="MMKLKLRTQFAILTIAVTLFPLVFGLLFFTGQQAKRDPRVTTRL
                     FIDEVAKEWILDNNISLEDIRSAGERVGLPIMDAALVDPDGTVRVSSFRDLPVGIKLD
                     VIDLARPPLLPYGKPKPELRFLPIDASLEKSPLLIFDIQPFLSREDIRNRNFMLVTSL
                     TLGALIVAGFMSFLILRSTEKAINNLIEDTAIVARGKLDHEVKGSGALELRSLADSIN
                     HMRITLRDMISRRMNMLIGVSHDLKTPIALIQGYTDALSDNMASDEATRIHYLEIIRE
                     KSAQLEDLVSELIEFMKIDDLNMPQEAVDLWPLLMSLGKRFEEDARLLGLELLYGFGH
                     FDSVLSKEEPKDLPRVRMNRALFERAVENLISNAFRYTGAGGHVELALDIKNGQPVLI
                     VADNGPGISEEDLPYIFDAFYRGTHSRKEAGHGFGLTIVKSVVDLHGWSIEARNRKTS
                     TQSQAENKMQEGSKGQGLVIAITMKPAIHD"
     gene            complement(7101..8402)
                     /locus_tag="PLS88_06680"
     CDS             complement(7101..8402)
                     /locus_tag="PLS88_06680"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOU07063.1"
                     /translation="MNIAEKKRESGIDRLELERLVDRTIKESGALDASKILIIPPDIT
                     RYHSKAGLITDLLVEQLGPRVAAILPAIGTHAPMTADEISQMYPLSPRTLFRVHDWRK
                     DVVELGKIPEERVEEISGGKVRYSYPIQTNKLLALGDIDLVFSIGQVVPHEVAGMANH
                     AKNIFVGTGGKEAIDKSHYLGATCGMESIMGRIDTPVRALFDEALSISKAKLPPIAWI
                     LTVIGTPRDGAPLQQGYFAGYDRRCFEEACTLSAELNITLFEKPIRKAVVWLDPSEYR
                     STWLGNKSIYRLRMAMADESELIILAPGLRSFGEDAAIDAVIRSFGYRPSGEIQNLVA
                     REPALSQNLSAAAHLIHGSSEGRFTISYAPGPGMTKEEIESVGYHYIDLEKAKSRYLP
                     EGRLAGAGFYNTLDGEEYFFVPNPALGLWSTAERMASVPKP"
     gene            8492..9727
                     /locus_tag="PLS88_06685"
     CDS             8492..9727
                     /locus_tag="PLS88_06685"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOU07064.1"
                     /translation="MLQLSRFSMGTGDRFGREGEAQIGAFQDLRAKGGEADIVWNKSN
                     REHVLIGSTPADQAKAASEAIRKTGWDGGWFIDADHITMKTVDWFLPYCNFFTIDVAE
                     SIGKKASSDSRLSFLDRAAFLLKDSSAPVWVENADLESVADKFLAAIEEAGKTYRYIA
                     ANKPKGSFVIELSMDETDKPQTPAQVAAILVAVAAEKIPISTFAPRFPGKFLKGIDYV
                     GNTAEFFHTFEEEAKVLLWASESLGLPKGLKLSVHSGSDKFKLYKGIHEIATRLGAGV
                     HLKTAGTTWLEEIVGLAEAGGKGLSLAKRVYEQAYLRIDELTAPYANVVEIDASSLPS
                     PQIVASWDSDAFVNALRHELDSSMMQPGMRQLMHVGFKIAAEMGKEYLDALEEYRESV
                     SRNVRYNLYARHLEPIIFG"
     gene            9675..11312
                     /locus_tag="PLS88_06690"
     CDS             9675..11312
                     /locus_tag="PLS88_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOU07065.1"
                     /translation="MSDTIYMQDTSSRSSSAEPSSSKTRPSLHRPGIMGKILICFGIV
                     IIFFLVLALVMQKESMNLSREYSKNLSNYLLVHRFRLNLNSFHAQADRYLREPSSVNI
                     DSIYDGILLLNTQYIELLPVADISIYIEFEVRATGYGLDAYLPLVSRSVGLRAGDSPD
                     YYQVFVKASRIHGYLDIYLNRMLSELMKNGEETYVSLSHKAELLNRTILISMILASTI
                     AIGVILFVTQAITAPLRRLAKEAEKLAKGDLDAGIVEVHTGDEVETLTRSFATMAKSI
                     KEYVEGLKEKSELERLLHEEELALVSMGKALREAQFLNLQEQMKPHFLFNALNTIARS
                     ALLENAARTESLAICLATLLRATIKDSGALSTLGEELVIAQAYLEFQQVRFGDRLQWN
                     IDVPQSLRKARVPRLMIQPIVENSVRHALEPKIEGGFISIKAQKRKEGLVLWFLDNGI
                     GMSKERLADVRAKIAASLQMHSFEQINLPQMVEIPSNGENNVNEEVLLEGRSIGLANI
                     ATRFGILYGDGCRFEISSRKEKGTLVRITLPLGENLG"
     gene            11309..12682
                     /locus_tag="PLS88_06695"
     CDS             11309..12682
                     /locus_tag="PLS88_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU07066.1"
                     /translation="MIRILVADDEELERKAMILILNSAPSNEIIDVLEASNGIEALDI
                     SQSQRVDIAFLDIHMPGIDGLRVAEKLSSLPNPPIIVMVTAYDYFSYARQALRYGVIE
                     YLLKPASTAEVYNALFLAFSEIKARKEEEKRQAEAKRVAADLKDFLRAKVYSALRENT
                     MDTEIIRQLVKLETGSETWLCIALVAGIKKFMSGDMFSLASSEFNRIFPILSEKFLIS
                     DIGLAESKAMLFVSPSEPRAEAMRGEQILDAIMNVLIILPYEINNGESDESFQEFFSR
                     YRKHFEEESIARFLCRCRDAGARTLRIGISAADYSLASTALRMATTAFNLSSVERPVI
                     VLNPTQTDELKVKDPHTVASLALLWLEDHFMENIGLKDLAMAINISPSHLSRLLMKET
                     GISFGEILSRIRIARAKSLLLNGISVKVASFLVGFRDQSYFTKVFTKIEGISPSHYIE
                     QFQSRSS"
BASE COUNT         3455 a         2811 c         3070 g         3425 t
ORIGIN      
        1 tcatgactgc gctggccaat tcagcgggca ctgtaatacg cgaagtcagc gtgaaaggca
       61 tcataaaatt caatcagagc aatgaacagc ttgcgggtat cactcttgtc gatgcggata
      121 caatgcgcga cctgttgggt tttgcaagct tgcgagatgg agctccccag ttgagcgagc
      181 acgaagcagc cttcttaacg gactttgatc ccgatagtct attcgataat ctattcgcaa
      241 gccagacgat tatgacagaa ggcgaagaac aacctgatat tgcttccggc ctttcttcag
      301 aggatatacc tgaaaacaca gggctaagaa cagctcaagt ctcgagctcc acaactgtga
      361 ctgcagagat tgtgccggct tggcaatttc ttcttgtgcg cgcaaaaaaa ggagccaaca
      421 cttccaagct tttggacgaa cttcaagtct tttccgaaca gcttggagat ggggatcgcg
      481 tgcaggattg ggttgcagga gctggaaagg tcgccaggac cgcagtaacg attcggcttg
      541 tattcgatct attggtggcg atcattgcag tcgtcgtaat catgataatg atgaatgtgc
      601 ttgtcatatc cgtcaatgag cgactccacg agatcggcac gcttagggcc ataggagcca
      661 agcggcatgt tgttcgcagt atgattctat atgagactac attcatggcg ctgatcgccg
      721 gagcagccgg tcttattcta gggtttatct tgctccttat catggggaaa gtcggcataa
      781 gagcgcctaa tctctttttc gaagctcttt ttgggggaga aatcttaaag ccagttatat
      841 caataggagc ggccattcgc gcttttcttt ggattctggc tatggccttg gctgcctcct
      901 ggtatccgac aagggtggcg gtacgaatcg agccagttgt cgcaatgaga ggagaatgaa
      961 tatgcacctt tcaaatctta agctccattt taagcttgcg gctcggaatg ttcgtagaca
     1021 gcccaataga acgattgccc ttgggggagc ggtggccttt agtgcactag ttatgactct
     1081 tatatctggg tttgtgagcg gaatggatac ggcaattcag gacaatgtga cgctttattc
     1141 aggggggcac atattgattt cgggctatac tcctagctgg agtggtaggc ttcagaatcg
     1201 attcgtcgat gatgagatag caacaattgt aggatccgct gttactgata tcaggacggt
     1261 ttctccgctt gctcaggcaa gagccacagt agtctttgga actcgagaaa tacagcttac
     1321 gctacgcggt atcgattgga caaaggatga gctatatcac gattccctcc tcttatcgag
     1381 cggagattgg aaagcactca acgagcatga tagaagcatg ctcatgagtt ctcagactgc
     1441 ggatcgattc ggtcttgcga ttggagatca ggtggtcgta aggctcacca cggcatccgg
     1501 tcagcagaat gttacagatt ataccgtttc ggcgatctat gacgatgccg catctggggt
     1561 catgacaacc gcttttgttt cttttgagga tttaatagcc gatctcaata tgaaaccaaa
     1621 tgaacagcaa caaatagcga tttttcttaa ggacagcacc aatgcagaac atgcggcaaa
     1681 ggctattgct acggcgcttg ccgaaaaggg ttatagcatt gcctctggaa gcgagcaggc
     1741 aactcagcgc cggatgaatt ccggaaaagc ggtctactcc atatctacag tacaagagct
     1801 cgcgggagaa ataggctccg ctctcggctc catccgttgg attgggtacg cagtgttcat
     1861 tcttatgctg cttgtcagtg cgacgggtat tgcaaattca tatcagatgg tgctcctcga
     1921 gcggactaaa gagataggta tgctgcgatg cattggtttc aagaagaaag atgtgtttgc
     1981 atcttttatc tccgaaggta ttttgcttgc aggagcagca gctattgctg gaattctatg
     2041 cgccctgccg ataggcctag gtattagctt tattcccttt gatccacatg gagactttgg
     2101 agcagctctg gtgaaaggac atttacgttt tattccgacc ctaggtcagc ttcttataat
     2161 cttggtgttt attatgattg tggctgttgt cgctgtcttt ttaccagccc gaaaagcggc
     2221 tgaagtagtg cccgtcgagg cactgaggat tactgcgtaa tgctagcaat attgcgattg
     2281 cgcaagaacg aggagcgatt gatgttgcga ttatacgaga aaaagacgaa gtacttccca
     2341 tcaaaaagca tcagggatat aagccttgta attttagggc ttgtccttgt tgtgtgtgcg
     2401 caagctcaag gtagcccacc tccaggcgga ccaaagggtg gtggaggacc tatggctggt
     2461 attgggcttc cacttggttt tctcgaagaa gataaagact acaaatttcc tgatgatata
     2521 acaaaatata cggcgaaaga ctttcttatg cttgttcgtc ggaccgatat ccgctcgagc
     2581 ttttacaaca gcgatatgag cgctaccatt acgacagtaa acgttagtcc gactcgcggt
     2641 acctttgtaa ccaaggaaca aatctttagg cgagataaag atgatgcatt cttgatgctc
     2701 accctcgagc ccgaatcccg taaggggcaa ggtatgcttc gagtgaacaa caatatgtgg
     2761 cgctacgatc ccacaagccg aaagttcact cacacaaccc ttaaagacac ctatgagaac
     2821 acgacagtaa caaataacga tttccgtcgc tggcagcgct cgattgatta ttctgtggag
     2881 aagctctcaa cgggtactct tggaaactac aaagtgataa tcggagaact aagggctaac
     2941 aatgacgaag ttccattccc ttacatcaag atgtatgtgg aagcagaccg aaagatagtg
     3001 cttaaggttg aggaatacag cctctctatg aaacttctcc gcaccgccta ctacactcag
     3061 tatgtgcaaa taggagattc ctttgtgcct acggtccaga tttttcagga tggacttgtg
     3121 cccgaaaagc gctctcaggt tacctatacc aatatttcca ctaagccgct ccccgactat
     3181 tactttacca aggaatacct cgagcgggtg agccagtagg agtagaggaa tgaaaacaag
     3241 aataattaag agcttgttat tccttataat gacgactctc tttcttttta gcaaatcaag
     3301 tatctgggct caatcctcaa gctcttcatc cagctttaag ccgcccgagg taatagatga
     3361 aagtgagtta tttggcggcc ccaaggatat tgtaacagtg atcgatccta gtgttgcttc
     3421 ggctggaacc gtgcagcttg tagaagacac caagacttac ccagtttttt tgataagtgg
     3481 atcagctgga gctggtctat atggcaacta tactcctcta gggggcagtt cctccgataa
     3541 acagacactc tatggagcgg tggatatttc cgatctcgaa ttcgattatc ttccagccga
     3601 gaacctacat ttttccttat ccagcaatct tctagccgtg cccaatcaga ttcttgctgc
     3661 atctgccagc gcttatgcgg atttgcgaca gagcgatcta gtaaggctct atgcctcggg
     3721 ttcattcaaa tacgatccat cgtcttattt atcgaccaat caagaatata tctctcctac
     3781 ctttagcctg gacgaactct ttgtagatac acatatagaa gagacagtct tctttcgatt
     3841 gggcaaacag cgcataagtt ggggagtagg gaattggttc aaaccctcgg atgttcttag
     3901 tctatcggct attgatccag acgacccaac cgcggcgcgc gaggggccct ttgcattcaa
     3961 ggtggatgtg ccgatgaagc taaatcacct tatggtgtat atggtgccac cgatttcaga
     4021 tgatgcggct tcgtcttcca ttgcgagtaa gtacgacctc gtagttaatg gatgggagct
     4081 cagttttggt gcctatgctc gggctgatat gttggcaaga cctagagcaa tcttcagttt
     4141 tactggggct attggttcat tcgatgtata tggagagaat gtactcttgt atggttccga
     4201 tcgaatctat gctaaagagt cttcaacacc tgggacttac gaagcttatc gtgtggaaaa
     4261 tgagcctttc tttcaatcta cattgggtgt caaatactct aagagctatt caggcggcct
     4321 atcgtattcg atacatgttc aaggttacta taatgggatg ggttatgcag attcaagcat
     4381 aatgcagagt gcagccgcca aagcagcaat aaaaaaagcg atagaagatg actatcctac
     4441 tgcttatagt tatagcgata gttctataga tgcgggcatg tggtatctcg ctggatccgc
     4501 atcggtatcg gggcgctttg gagaaggaaa caatctcacc cgtgtaacag gctccgtcta
     4561 tgctcttgcc aatttttcag atggttcgct gcgctttaat ccgcaactta aggtacaagt
     4621 aggagatgaa ggcggaaaag tgagctttac ggtttctgat ttgacttcta ttggcgatgt
     4681 tggttctgaa tatgcgaaaa atggcaatat aacgacacca gcactcaccg taagcctact
     4741 aaacagcgaa gtatcgctcc aggcctccgt gcccataaac ctcaatgcgg attattcggt
     4801 caagaagctc cagactaagt tcagtatttt ctggaatgca atagaatttt gatgccaaga
     4861 aatggagaat caatgcaagt ggcctggcac tttcgccagg tgtgcttgca taggagggga
     4921 gcatgcatgg aaaaatccta atcgtcgaag acaccaaaga gctcgctgag ctctacaagc
     4981 tctatcttca gaatgaaggc tttgaatgca agctcgcatt cgacgctgag agcgctcttc
     5041 ccattgcaca aaaagaggaa tgggatctcg tagttctcga tctcaatctt ccaggtatgg
     5101 atggctttca gttccttgag caacttagaa agacaaagtc catgcccgta atgattctaa
     5161 ccgcgagaga ggctgatgaa gatgtaattt ttgggcttgg cgtaggagcc gatgacttcg
     5221 tgactaagcc ctgtcctccg aaggtccttg ccgctcgcat ccgagctcat atgaggcgta
     5281 cagccaccct tcagatgcga caaaatggaa gcatggtaag atttggacct tatgagctag
     5341 atctcgaagg aatgtatcta actaaaaatg ccgagccaat cagcctttct gcgcgcgaat
     5401 tccacatctt aaaagaactt gtaagcaatc caggcaggcc atttactccc gaagacctct
     5461 atactcgcgt atggggtcag gaatatggcg atataagcgc tatcggagtc tatattcaaa
     5521 ggctgcgcaa gaagcttgag gacgatccag caaatcctta ttatatccag acccaatatg
     5581 gaatgggcta ccggttcaat cctgaagcct ttgtggagaa agcatgatga agcttaagct
     5641 ccgaacacaa tttgcgattc tcactatagc tgtgacactt tttcctctcg tatttggatt
     5701 gctttttttt acaggccaac aagccaagcg cgatcctcga gtaacgacac gactttttat
     5761 cgatgaggtg gcaaaagagt ggatcttgga caacaatatc tcactcgagg atattcggag
     5821 tgctggcgag cgggttggat tgcccataat ggatgccgct ctggtagacc ccgatgggac
     5881 agttagggtc tcttctttta gggacttgcc agtaggaatc aaattagatg ttatcgatct
     5941 tgcgcggcca cctcttcttc cttatggcaa gcctaaaccg gaactgcgct ttttgcctat
     6001 agatgcgagc cttgaaaaaa gccctcttct tatttttgac attcagccat ttctatcgcg
     6061 ggaggatatt cgaaaccgta atttcatgtt ggtgacgagt cttacgcttg gagccttaat
     6121 tgttgcgggt tttatgtcct tcttgatact tcgctccaca gaaaaagcaa tcaacaatct
     6181 aatagaagac acggctatag tcgcaagagg caagctcgat cacgaggtaa agggaagcgg
     6241 tgcgctagaa ctccgctccc ttgcggatag catcaaccac atgcgaatca cattgcggga
     6301 catgatttcc agacgaatga acatgctcat tggagtaagt catgatttaa agacacctat
     6361 tgcccttatc caaggataca ctgacgcact aagcgataat atggcctccg atgaagcaac
     6421 aaggatccat tacctcgaaa tcatccgaga aaagagtgcg cagctcgaag atctcgtctc
     6481 ggaattgatc gagttcatga agatagatga tctaaatatg ccccaagagg ctgtggattt
     6541 atggccgctt cttatgtctt taggcaagcg ctttgaggaa gatgctcgtc ttctaggtct
     6601 tgaactccta tatggctttg gacactttga ttctgttcta tcgaaagaag aaccaaaaga
     6661 ccttccaaga gtcagaatga accgtgcgct attcgagcga gccgtcgaaa acctaatatc
     6721 caatgcattc cgttatacgg gagccggtgg ccatgtagaa cttgctttgg atataaagaa
     6781 tggacaacca gtattgatag tggctgataa tgggcctggc atttctgaag aagacctacc
     6841 atacatcttc gacgcattct atagaggaac gcattctagg aaagaagcgg gtcatggttt
     6901 tggtcttacg attgtaaaat ctgttgttga ccttcatggt tggagcattg aggctcgcaa
     6961 tagaaaaact tcgactcaga gccaagctga aaacaaaatg caagaaggaa gcaaaggtca
     7021 aggccttgtg atcgcgatta ccatgaagcc agcgatacat gattagagtc tagaaaggtt
     7081 gatatttttg ctcaagaata tcaaggcttt ggaacactag ccatgcgctc tgcggtagac
     7141 caaagaccaa gggcgggatt tggtacaaaa aagtattcct cgccatccaa agtgttgtag
     7201 aagcctgcgc ccgcgagcct gccctcgggt aagtatctcg atttggcttt ttcaaggtcg
     7261 atgtaatgat agccgacaga ctctatttct tctttagtca tgcctggccc aggggcgtag
     7321 cttatagtga agcggccttc gctggatcca tgaattagat gggctgcagc cgagagattt
     7381 tgcgataggg caggctcgcg ggcgacaaga ttctgaatct cgcctgaagg cctataacca
     7441 aagcttcgta tcacggcatc gatagcagca tcctctccaa agctgcgaag cccgggcgca
     7501 aggattatga gctcgctttc atctgccatg gccattctta agcgataaat cgatttgttg
     7561 cctagccatg tgcttcgata ttcgcttgga tcgagccaga ccaccgcttt tctaatcggc
     7621 ttttcaaaaa gcgttatgtt cagctctgca gaaagggtac aagcttcttc gaagcatctt
     7681 ctatcgtatc cagcaaaata tccttgctgt aaaggcgcgc catctctagg cgtccctatt
     7741 acagtaagaa tccaagctat tggaggcaat tttgccttag aaatactcaa ggcctcgtcg
     7801 aaaagagctc tcactggtgt atctatacgc cccataatgc tttccattcc acaggtggcg
     7861 cctaaataat ggctcttatc gattgcttct tttcctccgg tgcctacaaa aatatttttt
     7921 gcatggtttg ccattccagc aacctcatga ggcacgacct ggccaatcga aaagacaagg
     7981 tctatatctc ctaaagcgag cagcttattt gtttgaatag ggtaggaata tctaacctta
     8041 ccgccggaaa tctcttcaac gcgctcttct ggaatctttc cgagctcgac tacatccttt
     8101 cgccaatcat ggactctgaa tagtgttcta ggcgaaagag gatacatctg tgagatttca
     8161 tctgctgtca tgggtgcatg agtgcctatg gctggtaaga tcgccgcaac acggggacca
     8221 agctgctcga ccaatagatc ggttatcagt cctgccttgg agtgatatct tgtgatatct
     8281 ggaggaatga tcaaaatctt gctagcatcg agcgcacccg attcttttat agtcctatcg
     8341 accaatcgtt cgagttctaa tcgatctatg ccagattctc gctttttctc agctatattc
     8401 attgtagaat tcctttattc gaaaattcaa aaagagcttg cgtacctttc tttcaagcga
     8461 cattatatag aaaacaactc aggagggaca tatgctgcaa ctttctcgct tttccatggg
     8521 cactggagac cgatttggtc gagaaggaga agctcaaata ggcgctttcc aggacttgcg
     8581 agctaaagga ggcgaagcgg acatcgtttg gaacaagagc aatcgcgaac atgtgctgat
     8641 aggctcaacg cctgcagacc aggcaaaggc cgcatcggaa gcgataagaa agacaggttg
     8701 ggatggagga tggttcatag atgcagatca tataaccatg aagactgtcg attggtttct
     8761 gccatactgc aattttttta ccatcgatgt agcggagagc ataggaaaga aagcttcatc
     8821 agattcgcgt ttatcttttt tggatagagc agcttttctg cttaaggata gttcagcgcc
     8881 tgtctgggtt gagaatgctg atttagagag cgtagcggat aaattccttg cggctatcga
     8941 agaagctgga aagacttacc gctatatcgc agcgaataaa cctaaaggtt cttttgttat
     9001 cgagctatcg atggatgaaa ctgataaacc acaaactcca gcgcaggttg cggcaattct
     9061 tgttgctgtg gccgcagaga agattccgat ctcgactttt gccccacgtt ttccgggtaa
     9121 attcctaaag ggtatcgatt atgtaggaaa cacagctgaa ttcttccata ctttcgaaga
     9181 ggaagctaag gtccttctat gggcttccga atctttaggt cttccaaagg ggctaaagct
     9241 ttcagtacat tctggtagcg ataaattcaa gctttacaag ggaatccatg agatcgctac
     9301 aaggcttggg gctggcgttc atctcaagac tgcgggtact acttggctcg aagagattgt
     9361 tggccttgcc gaagcaggag gtaaaggcct ttcgcttgca aagcgtgtat atgagcaagc
     9421 ctatcttcga atcgacgaac tcacggcccc ctatgccaac gttgttgaaa tcgatgcctc
     9481 tagcttgccc agtcctcaga tagtagcaag ctgggattcg gatgcttttg taaatgcttt
     9541 gcgtcatgag ttggattcat ctatgatgca gccaggcatg agacagctca tgcatgtggg
     9601 attcaaaatc gcagcggaga tgggcaaaga atatctcgat gctctagaag aataccgcga
     9661 atctgtatct cgcaatgtcc gatacaatct atatgcaaga cacctcgagc cgatcatctt
     9721 cggctgaacc atcatcatcg aagaccaggc caagtcttca tcgacctggc attatgggga
     9781 agattctcat ttgctttggg atcgtcatta tcttctttct ggttcttgcg ctcgtcatgc
     9841 agaaagaatc gatgaatctc tcgcgcgaat atagcaaaaa cctatcgaat tatctactcg
     9901 tgcatagatt taggctcaat ctaaactcat ttcatgccca ggcggatcga tatcttcgtg
     9961 aaccttcttc ggtaaatatc gactctatat atgatggcat attgctttta aatacacagt
    10021 atattgagct ccttccagtc gccgatattt cgatctatat tgaatttgaa gtacgagcaa
    10081 ctggctatgg cctcgatgct tatcttcctt tggtatcgcg ttcagttgga ctgagagctg
    10141 gggattcacc agactattat caggtctttg tcaaggcaag ccgaattcat ggctacttag
    10201 atatttatct caacagaatg ctttccgaac ttatgaaaaa tggtgaagag acttatgtta
    10261 gtctctcgca taaggcagaa ctcctcaatc ggactattct tatctctatg attcttgcga
    10321 gcactattgc cataggggtc attctttttg tgactcaggc aattactgct cccttgcgaa
    10381 ggctagcaaa agaagcggag aagcttgcca aaggcgattt ggatgcgggt atcgtagagg
    10441 tgcatacggg cgatgaagtg gaaacactga cgcgttcttt tgcgacaatg gcaaaaagca
    10501 tcaaggaata tgtagaagga ctcaaggaaa aatcagaact cgaaaggctt ttacatgaag
    10561 aggagcttgc tcttgtgagc atgggtaaag cacttcgtga ggctcagttt ctaaaccttc
    10621 aggaacagat gaagcctcat tttttattca atgccttgaa tactattgcc cgtagcgcgc
    10681 ttttagaaaa tgcagccaga actgaatcgc ttgctatctg tctagcgaca ttattgcgcg
    10741 caaccataaa agatagcgga gcgctctcta cactaggaga ggagctcgta atagcgcagg
    10801 cctatctcga attccaacag gtgcgatttg gagataggct tcaatggaat atcgatgtgc
    10861 cgcagtctct gcgtaaggcg agggttcccc gtctaatgat tcagccgatc gtcgaaaact
    10921 cggttcggca tgcgctcgaa cctaagatcg agggcggctt catatcgata aaggcacaga
    10981 agcgcaagga agggcttgta ctgtggtttt tggataatgg cattggcatg tcaaaagaac
    11041 gtcttgccga tgttcgagca aaaattgcag cttccttgca gatgcatagc tttgagcaga
    11101 ttaatcttcc ccagatggta gaaatccctt caaatggaga gaacaatgtc aatgaagagg
    11161 ttttgctgga aggaaggagt ataggccttg ccaatatcgc gactcgcttt ggcattctct
    11221 atggtgatgg ctgtcgcttc gagattagct cgagaaaaga gaagggcact cttgtgagaa
    11281 ttactctgcc gctaggagaa aacctgggat gattcgtatt ctcgttgcag acgatgaaga
    11341 actagaaaga aaggcaatga tcctaatttt gaattccgcg ccatcgaatg agatcataga
    11401 tgtgcttgaa gcttccaatg ggatcgaagc cctggatatc tcgcaatcgc agagagtcga
    11461 catcgcattt ctagatatac atatgccagg aattgatgga cttagggttg cagagaagct
    11521 atcatctctt cctaacccgc ccataattgt aatggtgaca gcctatgatt atttttccta
    11581 tgctcgccag gccttgagat atggtgtcat agaatatctt cttaagccag catcgacagc
    11641 agaagtctat aatgcgcttt ttctagcgtt tagcgagata aaagctcgga aagaggagga
    11701 gaaaaggcaa gcggaagcta agcgtgtagc agctgactta aaagactttc tccgagcaaa
    11761 agtctattcg gcattacgcg aaaatacaat ggatacggag atcattcgac agctggtaaa
    11821 gctggaaaca ggaagcgaga cctggctctg catagctctt gtcgcaggaa taaagaaatt
    11881 tatgtctggc gatatgtttt ctctagcctc aagtgaattc aacagaatct tccctattct
    11941 ttctgagaaa tttttaatat ccgatattgg gcttgctgaa tccaaagcaa tgcttttcgt
    12001 ctcacccagc gagccgagag cggaagcgat gagaggcgag caaattctgg atgcaataat
    12061 gaatgtattg ataatcctcc cctatgagat aaataatggt gagagcgatg agagtttcca
    12121 agagttcttt tctagataca gaaagcattt tgaagaagaa agcattgcac gcttcctttg
    12181 ccgctgtcga gatgccggag ctagaacact tcggataggt atcagtgccg cagactatag
    12241 tttggcatcg acagctctca ggatggccac aactgccttc aaccttagct ctgtagagcg
    12301 gccagtcatc gtattgaatc cgacacagac cgatgaacta aaggttaagg acccccatac
    12361 ggttgctagc cttgcattgc tgtggcttga agaccatttc atggagaaca ttggtcttaa
    12421 ggatttggcc atggccatca atataagtcc ttctcatctt tcccgattgc ttatgaagga
    12481 gactggaatt agttttgggg agatcctatc ccgaatccga atagcacgag cgaaaagcct
    12541 gcttttgaat ggtatctcgg taaaagttgc atcatttctg gttggctttc gcgatcaaag
    12601 ttatttcaca aaagtattta ccaaaatcga aggtatctcg ccatcgcact atatcgaaca
    12661 attccaatcc agatcgagct agagtctttc caagcatcat attgctaaga ataggcatgg
    12721 aattaccaaa cacaaaacga aaaatagcct cctataatta c
//
LOCUS       DAOSOB010000056        11862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_5353_length_11862_cov_75.221479, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000056 DAOSOB010000000
VERSION     DAOSOB010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11862
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5353_length_11862_cov_75.221479"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PLS88_06700"
     CDS             <1..1322
                     /locus_tag="PLS88_06700"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOU07067.1"
                     /translation="LKFELSHSLFSSFDIDEGTRFLLKEIANEPGIIHAHRLLDAGCG
                     VGVIGISLAASCPEMDVVLRDRDFRAVVFSARNAERNGLGIRLFGLDGQEHALCHKKR
                     FAKIKIKERKADYLIAEPGLLCEPDERGPYDAVVSNLPAKAGVKVLENYFHFVCTELL
                     QPGGTFAFVIIAPLADMACIWCAKSGLTLLRAISTKNHMVAIAQSPKEVQVGKTSQER
                     WISTYFRSHTEKQLARASLDWDGFLGLPEFDEPSFATVCALSLAQKVFAGLLVRRALV
                     VEPGVGIAPMWIKKVLGTEEIVLISHDVLALTASIHNLEKMSSLKTIAMLDPVLPIIS
                     PSVSEKSPAQETLSSQKEGQVSAWQELSPASIDVAIFFIEDIPKYNIVSTYWLLAEKV
                     LKRAGVVIFSGTSTACERVGREKPLGFSKLPYIDQKKGWHAIAFRRD"
     gene            1465..1965
                     /locus_tag="PLS88_06705"
     CDS             1465..1965
                     /locus_tag="PLS88_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07068.1"
                     /translation="MILESKTPDEYVANSLKSRLSPAEKAKLARLWMQETGFKREDII
                     HARNRSEYWQKRKMAGATERTKRRMAQHDYSNGKTIIWTREDLAEFLSLNRKDTYGRY
                     LYRDWELAAHFGTSIPSIQYLRRKCNKVRKMLGPGARRDKIIDYMSCSELVLQRGGPK
                     ALKRKR"
     gene            complement(1962..4019)
                     /gene="recG"
                     /locus_tag="PLS88_06710"
     CDS             complement(1962..4019)
                     /gene="recG"
                     /locus_tag="PLS88_06710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOU07069.1"
                     /translation="MFLRELTQDIRILRGTGPATLRSLANLGIHTIADLLLHLPRDYE
                     DRTKILPLSRFSEAKVYTKAKVIRHEWFGFGKMRTLKIIIADDSSEGALLCFNRPFLE
                     QMAPVGSIIQIYGKFQFKYGEIQSSAFELKPLQEDFVPEQNLSPIYPLTGRLNQTTMR
                     RLTALALQKFAPHIEDELPDALRESYCFPPKSEAIRSIHFPSSWESVKIAHDRLAYEE
                     LFYFQLGIALRIRSRQTKTINRIFVPGVLARQLRERLPYTLTEDQELVLKEIIEDMHK
                     PFPMARLLQGDVGSGKTMVALLAALYAVERGGQVAIMAPTELLARQHATIAAHLVEPL
                     GVRLAFVTGSISSAVRPQLLEALKSGDIDIAIGTHALFSDDVSFKNLELVIIDEQQRF
                     GVLQRVALYKKGHVPDFLMMTATPIPRSLALTFFGDMQVSTIQHLPPGRKPVITHLAS
                     EVRRGSVYEFTRKKLQEGQQAYFVYPVISESDNLSLRNAESMAEHLSKEIFPEFEVGI
                     LHSRLSDDSKMDVMSRFSAGEIAVLVATTVVEVGVDVPNATVMVVEHAERFGLSALHQ
                     LRGRIGRGGLQGYCFFVYSKDITEEGRMRLKALYETNDGFAIAEEDLKIRGPGNIFGL
                     EQSGDLQLRIADMRTDFDLLKRARHDAFRIIENDPGLTSPENNVIRQVLNRANPYSEK
                     QES"
     gene            4093..5592
                     /locus_tag="PLS88_06715"
     CDS             4093..5592
                     /locus_tag="PLS88_06715"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOU07070.1"
                     /translation="MQKETARLIELDREHTLMSHISAVLGWDQETYMPSKAIQERASQ
                     LALFEGLAHQKAVNPEIGELLATLEAKHDLSPDEKAYIRKVRRDYDIETKLPEAFVTE
                     YARSTSIAQVKWAEAKKDNDFKAFESHLEKIVGLSRELAGYLNPNARPYDVLLDLYEP
                     GSSQESITAVFSTMKAYLVDILDKIRAKPQIEDKCLGRYVSSETQEQISLYFMKVLHF
                     EMDRGRLDVSAHPFTTSLGADDIRITTRYVEDYFPSSLFSTIHESGHALYDMGIDPNP
                     EFRGTKLAEAVSMAVHESQSRLWENIVGRSRAFWERNFLALSALLGEAGRDLDFESFI
                     ESVNRVSPSLIRTEADEVTYGLHIIARFELESALLDGNLRVADMPGAWGEKYKKLLGL
                     EVPNDRVGCLQDVHWSIGYFGYFPSYALGNLYAAQFWAKLKEEVPDIEARIASGDTLS
                     VLTWLRANVHCHGSRYMPGELVEKATGSALDPVWFEKYLREKYSSIYGF"
     gene            complement(5596..7026)
                     /gene="hisD"
                     /locus_tag="PLS88_06720"
     CDS             complement(5596..7026)
                     /gene="hisD"
                     /locus_tag="PLS88_06720"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOU07071.1"
                     /translation="MEKHPLRRIRIDDIPEPSARLFDPEIDAVAKQAIDAIRTGKEEE
                     LRRWAERLDGLPAGAPLVISCDEMKAAYDALPKETAELLGRAKGRVAAFAAAQRACLA
                     PLSIPLQIEPIEIASPIDPIEPKSLLEPNEPADIEMSAKKMTAKGIKKEGEKGALCGR
                     YGHEIVPVARVGCYVPGGAFPLPSSAIMTVVPAKVAGVEEVWCAGPNPSKETLAASWL
                     AGAEGFLQVGGAHAIAALAFGVCIPKCDVIVGPGGKYVASAKRQLFGIVGTEAPAGPS
                     ELLIIADKTAEADIIAADLLAQAEHSPDSLPAAIVTEETLALEIQKALKTRLEELPEP
                     NRSIANRALSNGFICVEHDLLRVVDGANRCAPEHLEIATTDPASLMKQIKNAGAVFLG
                     LRSAEVFGDYGAGPNHTLPTGGASRFAAGLSVLCFLRTRTWLAIENPELLVEDTAAFA
                     RLEGLEAHAQAALTRTLKHKNESPKS"
     gene            complement(7008..8321)
                     /gene="hisE"
                     /locus_tag="PLS88_06725"
     CDS             complement(7008..8321)
                     /gene="hisE"
                     /locus_tag="PLS88_06725"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOU07072.1"
                     /translation="MIVPSIDIMNGRAVQLRGGKQPPLDAGNPLELAERYARVGEFAV
                     VDLDAAKGIGSNRELILSLCRTHRVRVGGGIRTIGLAMDYLNEGARSIMIGTSATPEF
                     LSSLPRDRLIAALDSKEGTIMVEGWSKPREGSIEQSISVLSPYVSGFLITFIETEGDQ
                     CGIDIERARKLIALSPDRKFTFAGGAAGGERGIADIAALDRLGADLQVGTSLVLGDLS
                     LAEAFAAPLVTDRADGLWPTLVCDESGKALGLVHSSMESLKVALNSGKGTYHSRSRGL
                     WVKGESSGNDQTLIRAELDCDRDTIRFTVRQNGEGFCHLGRRTCFDNGYGLEKLDRTI
                     KKRMKEAPPGSYTRRLFADESLLASKLREEVQELAQAKSIQEAISEAADVLYFTLVKT
                     ISMGASLSDIEEELERRSLRVTRRGGDAKPEFEGPEGKSEWKSIH"
     gene            complement(8318..9127)
                     /gene="hisF"
                     /locus_tag="PLS88_06730"
     CDS             complement(8318..9127)
                     /gene="hisF"
                     /locus_tag="PLS88_06730"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOU07073.1"
                     /translation="MNTNKKSKSALQKCMRMSAVKIIPCLDFKEGRVVKGKRFQNLVD
                     SGDPLERALIYEKQGADELAFLDISATIEGRKTMAQEVARIHQAISIPLTVGGGIASI
                     EDAARLFEAGADYVSINSVAVKKPAIIDEMASIFGTECIVVAIDAAHNASMPSGYEVK
                     IDGGSKSMGIDVSEWAQEAAERGAGQILLTSIDRDGTCLGYDCRLIEIVARIVSVPII
                     ASGGASMLEHFLEGYLSGASALLAAGIFHRGEVSIREIKEFLKAHDVEVRL"
     gene            complement(9124..9726)
                     /gene="hisB"
                     /locus_tag="PLS88_06735"
     CDS             complement(9124..9726)
                     /gene="hisB"
                     /locus_tag="PLS88_06735"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOU07074.1"
                     /translation="MNDQPIATIRRTTAETDVGVSLQLSPGSIDINTGIGFFDHMLTQ
                     LAFHAGWSLKISCKGDLDIDDHHTVEDVGIALGIAFSKIVPSAKGLARFGYSYAPMDE
                     ALARAALDISGRSYFVFSGDFSTPSIGSLSTCNIEHFFQSFATNARCTLHLDILRGEN
                     DHHRAEALFKAVALAFRAALAPSAALASTKGETCIDSEQT"
     gene            complement(9723..10775)
                     /locus_tag="PLS88_06740"
     CDS             complement(9723..10775)
                     /locus_tag="PLS88_06740"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOU07075.1"
                     /translation="MLRLDANEGRCILSEEDLREILSPEIARRYPMRELLEIPLAEKL
                     GLPSSCVLATAGADDAIDRTIRSLAGPGGLIMSTSPGFVEFLEAAQRSSARFKEIKKD
                     PFEPFPIDDICRCVRALTPRLLILSSPDNPSGVVLSPLDVTQIATVCQELGTIFVFDA
                     TYQEFSIHGSTPIQALANPNVLVTGSFSKSKGLAGFRIGYVAANPEFTQLISRLSEAG
                     PPYSLSSSAIEAGRRALSLDQGVAQGFIDEVRREVQTLKSLCRKLGFLVSESEANFIL
                     LKSENPTDLSDKLRERGILVRTWPRKPGYEELVRITVPGDQGEFEELCGSLEDAVREY
                     DKNNRIKSKPKNKERL"
     gene            complement(10793..11734)
                     /gene="hisG"
                     /locus_tag="PLS88_06745"
     CDS             complement(10793..11734)
                     /gene="hisG"
                     /locus_tag="PLS88_06745"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOU07076.1"
                     /translation="MTESANEKVELRLGLPKGRMQAKVIELFNDAGIPVSIDEREYRP
                     RIGNGKAVVFGSREMVYEAKLLKPQNIVEMLQAGSRDMGFAGADWVCELGADLIELLD
                     TGLDPVKIVAAAPENVATAFASSRGDLTKMIAACKRTPIVASEYEGLAKAWIDINLPG
                     AEFVHSYGATEVFPPEDADIIIDNYATGSTLRANRLEILDTLMTSSTRFYASKTAIAD
                     EDKREAAEALVLLFRSVLEARRRVMLEVNISAELLDELVAILPCLRAPTVSPLGGGDA
                     FAVKVAAPRDMLASLIPEIKRRGGTDIVATQISQLVP"
BASE COUNT         3047 a         2802 c         2845 g         3168 t
ORIGIN      
        1 agctcaaatt cgagctatct cattcgcttt tttcttcttt tgatatcgat gaagggaccc
       61 gctttcttct aaaagaaata gctaatgaac caggaattat tcatgctcat cgccttctcg
      121 acgcgggctg cggggtaggt gtaataggca ttagcttggc agcctcatgc cctgagatgg
      181 atgtggtttt gcgcgaccgc gattttaggg cggttgtatt ctccgcacga aatgcagaac
      241 ggaatggtct tggcatacgg cttttcggct tggatgggca ggagcatgcc ctatgccata
      301 agaaaaggtt tgcaaagata aaaataaagg agagaaaagc agattacctc attgcagaac
      361 caggtttgct ttgcgagcca gatgagcgtg gcccatacga tgcggtcgta tcaaatctgc
      421 cagcaaaggc cggagtaaaa gtacttgaga attattttca ttttgtatgc accgagcttt
      481 tgcagcctgg aggtactttt gcctttgtca tcatagcacc tttggcagat atggcttgta
      541 tctggtgcgc taaatcaggc cttactctat taagagcgat cagcacaaaa aatcacatgg
      601 ttgcaattgc gcagagccca aaggaagttc aggtaggtaa aacctcacag gagagatgga
      661 tttctactta ttttcgttcg catacagaga aacagcttgc tagagcaagt ctcgattggg
      721 atggttttct aggcttacct gagttcgatg agccctcttt tgctactgta tgtgctctct
      781 ctttggctca gaaagttttt gcaggtcttc tcgtgcgtcg agcacttgtt gtagagcctg
      841 gcgtaggcat cgcacctatg tggataaaaa aagtcttggg aaccgaggaa attgtgctga
      901 tatcgcatga tgtactggca cttacagcat cgatccataa tttagaaaag atgagttctc
      961 taaaaaccat tgccatgctc gatccagtgc ttcctattat ctcgccttca gtaagcgaga
     1021 aatcaccagc acaagagact ctatcatcac aaaaagaagg acaagtttca gcatggcaag
     1081 agctcagtcc ggctagcatt gatgtagcta ttttcttcat tgaagatatt ccaaagtata
     1141 atatagtaag tacatactgg ctgcttgccg aaaaagtgct caaacgtgct ggtgtggtga
     1201 tattttctgg gacaagtaca gcttgcgagc gagtcggccg cgaaaaacct ctaggcttct
     1261 ctaagcttcc atatattgat cagaaaaaag gttggcatgc catagccttt cggcgagact
     1321 aggccgatga gagattataa gaggccaact aatgttgaag ccatgagata cataagggtt
     1381 gctgacgcct cttgcagcta acttaggata tttgactttt agcaattctt tattaaattt
     1441 cataatatga aaaaagctga gcgtatgatt cttgaatcta aaacaccaga tgaatatgta
     1501 gcgaattctc tcaaatcacg attatctcct gcagagaagg cgaagttagc tcggttatgg
     1561 atgcaagaga caggctttaa gagagaagac attatacatg ctcgcaatag aagcgaatac
     1621 tggcaaaagc ggaaaatggc aggagcaaca gagcggacaa aaagaagaat ggctcaacat
     1681 gattactcaa atggcaagac tattatatgg accagagaag atctagccga atttctatct
     1741 cttaatcgca aagatacata tgggcgctat ctatatcgcg attgggaact cgctgctcac
     1801 ttcggtacta gtataccatc gattcaatat ctaagacgga aatgtaacaa ggttcgcaag
     1861 atgcttggtc caggcgcaag gagggataag attatcgatt atatgagttg ctccgagctg
     1921 gtgcttcaac gtggaggacc aaaggcacta aaacgcaaac gctaagattc ttgcttctca
     1981 gaataagggt ttgcgcgatt tagaacttgg cgaatcacat tattctcggg gctcgtaagc
     2041 cctggatcat tctcgatgat tctaaaagca tcatgtctcg ctctcttgag aaggtcaaaa
     2101 tcggttcgca tatcggcaat gcgtaattgg aggtctcctg attgctccaa gccgaatata
     2161 tttcccggtc ctcgtatttt caagtcttct tcagcaatgg cgaagccatc gttggtctca
     2221 tagagagctt tcaatctcat tcgcccttct tctgtaatat cttttgaata aacaaagaaa
     2281 caatagccct gaagaccgcc tcttcctata cgtcctctca actgatgcaa agcggacagt
     2341 ccaaaacgct ctgcatgctc gacaaccatt acggtcgcat taggcacatc gacaccaact
     2401 tcaacgacag tggttgcaac cagaacggct atttcgcctg cagaaaatct cgacatcaca
     2461 tccatcttag aatcgtcgct caagcgcgaa tgcagaattc caacttcaaa ctcaggaaaa
     2521 atctctttag ataggtgttc tgccatcgat tccgcattcc taaggctgag attatctgat
     2581 tcactgataa ctggatatac gaaataggct tgttggcctt cttgcagttt tttgcgtgta
     2641 aactcgtata cggaacctct tcggacttcg ctcgctagat gtgttataac gggtttgcgg
     2701 ccaggcggta gatgctgaat cgtagatact tgcatgtcgc caaaaaaagt aagagcaaga
     2761 ctccttggta taggtgtggc agtcatcatt aaaaaatctg gcacatggcc tttcttgtag
     2821 agggctaccc tctgtagcac tccaaagcgc tgctgctcat cgattatgac cagttcgaga
     2881 tttttaaaag aaacatcatc ggagaaaagg gcatgtgtac ctatggcgat atctatgtcg
     2941 ccacttttaa gagcttctaa tagttgaggc cgaacagcgc tagatatcga gccggtcaca
     3001 aatgcaaggc gtacacccaa gggctctaca agatgtgcag caatggttgc gtgttgccgg
     3061 gcaaggagtt cagtaggagc cattattgct acctgtcctc ctcgctcaac tgcatatagt
     3121 gccgcaagca gagccaccat tgtttttcct gatccgacat ctccctgcag aagcctggcc
     3181 ataggaaaag gcttgtgcat gtcctctatg atttccttga ggactagttc ttgatcttca
     3241 gttagcgtgt agggaagacg ctcccgaagc tgtctggcaa gtacgccagg aacaaagatg
     3301 cgattaatgg tttttgtctg tctagaacga attcttagtg caataccaag ctgaaagtaa
     3361 aagagctctt cgtaggcaag cctatcatga gcaattttaa ctgattccca tgatgaagga
     3421 aaatgaattg agcgaatagc ttcagacttg ggtgggaagc agtatgattc acgcagagca
     3481 tctggaagtt catcctcgat atgaggtgca aatttttgaa gtgctagagc tgtcaacctt
     3541 ctcatagttg tttggttcag ccttcccgtg aggggataaa taggcgaaag attctgttct
     3601 ggaacgaagt cttcctggag gggttttagc tcaaaggcag aagactggat ttcgccatac
     3661 ttaaactgaa atttgccata gatctggatt atcgagccaa cgggagccat ttgctcgagg
     3721 aaaggcctat tgaagcagag gagggccccc tcggaagaat cgtctgctat gatgatcttt
     3781 agagtgcgca tttttccaaa gccaaaccat tcatggcgaa tgaccttggc cttagtataa
     3841 accttagctt cagaaaagcg cgataatgga aggatttttg ttctgtcttc ataatctctt
     3901 ggcaggtgca gcaaaagatc cgcaatagta tgaataccta gattcgcaag tgaacgcagg
     3961 gttgctggcc ctgttcctct gagaatgcgt atatcctgtg tcaattccct taaaaacacg
     4021 gattttagta tacaaagagt cttattcgag tgctactata tgaattatga agagtaaaca
     4081 aggaggaaat cgatgcaaaa agaaacggct cgccttatag agcttgatcg agaacacact
     4141 cttatgtctc atattagtgc agtcctcgga tgggatcagg agacctacat gccttccaaa
     4201 gcgatacagg aacgcgcatc acagcttgcg cttttcgagg ggcttgccca ccaaaaggca
     4261 gtaaatcccg aaataggtga gctacttgcg acactagaag cgaagcatga tctttctcca
     4321 gatgagaaag cctatatccg caaggtgcgg agggattatg atatagaaac caagttacca
     4381 gaggcttttg tcaccgaata tgcgagatca actagcattg cgcaggtaaa atgggcagag
     4441 gcaaaaaaag acaacgattt taaggcattt gaatctcacc ttgaaaaaat tgttggcctc
     4501 tcgcgagaac tggcagggta tctcaatccg aatgcaagac cctatgatgt actactcgat
     4561 ctatatgagc ccggaagctc gcaggaatcg attacagctg ttttttccac aatgaaggct
     4621 tatctcgtcg atatcctcga caagatacgc gctaagccgc agatagaaga caaatgtcta
     4681 gggcggtatg tcagctctga aactcaagaa caaataagct tgtatttcat gaaggtgctg
     4741 catttcgaga tggatcgcgg aaggctggat gtttctgctc atcctttcac aacaagcctc
     4801 ggtgctgacg atatacgcat tacaacgcgc tatgttgaag attattttcc ttcaagcctc
     4861 tttagcacaa tccatgaatc gggtcatgct ctctacgata tgggaatcga tccaaatcca
     4921 gagtttagag ggacaaaact ggctgaagct gtatcgatgg cggtgcatga gtcacagtct
     4981 aggctgtggg agaatatagt tggccgctcc agggcattct gggagaggaa ttttttagcc
     5041 ttgagtgccc ttttgggtga ggctggcagg gatcttgact tcgaaagctt tatagaatca
     5101 gtaaatagag tgagtccttc gcttattaga accgaagcag atgaagttac atatggtttg
     5161 catataattg cgcgttttga gctagagtca gcgctattag acgggaatct gcgtgtggca
     5221 gatatgcctg gagcatgggg agagaagtac aagaaattat tggggttaga agtgccaaat
     5281 gatcgagtgg gctgccttca ggatgttcat tggtctatag gatattttgg atatttccca
     5341 agctatgcgc ttggaaatct ctatgctgca cagttctggg ctaagctcaa ggaagaagta
     5401 cctgacatcg aagctcgcat agcgagcggt gatactctct ctgtgcttac atggcttcga
     5461 gccaatgtgc attgtcatgg atcacgctat atgccaggcg aactcgtcga aaaagccact
     5521 ggttctgctc tggatcctgt gtggtttgag aaatatctac gagaaaaata ttctagcata
     5581 tacgggttct aagcattagg acttagggct ttcattttta tgtttgagag tacgtgttag
     5641 tgcggcttgt gcgtgcgcct cgaggccttc taggcgagcg aaagcagcag tatcctcaac
     5701 gaggagctct ggattctcta tggcaagcca tgttctggtt cgtaaaaagc aaaggactga
     5761 gaggccagcg gcaaatctag atgctcctcc agtaggaaga gtgtgattgg gccctgcacc
     5821 atagtctccg aatacttctg ctgatcttag gccgagaaag acagcacctg cgttttttat
     5881 ctgtttcata aggcttgcag gatctgtggt tgcaatctcg aggtgttccg gcgcgcagcg
     5941 atttgctcca tcaacaactc gcagaagatc atgctctaca cagataaagc cattcgaaag
     6001 ggctcgattg gctatacttc gattaggttc agggagctct tcgagcctag ttttgagggc
     6061 tttttggatt tcgagagcta aagtttcttc ggtcacaatt gcagcgggca gtgaatctgg
     6121 ggagtgctct gcctgggcta gaagatcagc agcaataatg tcggcttctg cggttttatc
     6181 tgcaatgata agtagttccg aggggccggc tggtgcttca gtgcctacaa tgccgaaaag
     6241 ttggcgcttt gctgatgcga catattttcc gccagggcct acgatgacat cgcatttggg
     6301 gatacagacg ccgaaagcta aggctgcaat agcatgggcg cctcctactt gcaagaatcc
     6361 ttctgcgcct gcaagccatg aggcagccag ggtctccttg ctgggattgg ggccagcaca
     6421 ccagacttct tcgacgcctg caacttttgc agggacaacg gtcatgattg cgctggaggg
     6481 gagggggaag gcaccacctg gaacataaca acctacacgc gccacgggca cgatttcgtg
     6541 cccgtagcgg ccgcacaacg cgcctttctc gccttctttt ttaattcctt tagctgtcat
     6601 tttttttgcc gacatctcta tgtcggctgg ctcatttggc tctaacaggc tctttggttc
     6661 tattggatct atcggagatg caatctctat cggctctatc tgaagcggta tgctaagagg
     6721 cgcaaggcag gcgcgttgag cggccgcaaa ggccgctacg cggcctttgg cgcgccctaa
     6781 aagctcggcg gtttccttag gcagcgcatc ataggcagct ttcatttcat cgcaagagat
     6841 aacaagaggc gctcctgccg gaagtccgtc gagtctctcc gcccatcgcc taagctcttc
     6901 ctctttgcca gtacgaatcg catcgatagc ctgcttggca accgcatcga tttctggatc
     6961 gaaaagcctc gccgaaggct ccgggatatc atcgatccta attcttctta atggatgctt
     7021 ttccattcgc tttttccttc tggaccttcg aattcgggtt ttgcatctcc cccgcgccgc
     7081 gttacacgca atgaccgacg ctcgagttcc tcctcgatat ccgataggct ggcccccatg
     7141 ctgattgttt ttaccaaagt gaaatagagt acatcggcag cttcagatat tgcctcttgt
     7201 atagattttg cctgggcaag ctcttgcact tcttcgcgaa gcttggatgc cagcagagat
     7261 tcatctgcaa atagccttcg agtataagag ccgggaggtg cttctttcat gcgtttcttg
     7321 atagtgcgat cgagtttttc cagtccataa ccgttatcaa agcaggttct cctgccaaga
     7381 tgacaaaagc cttcgccatt ctggcgcacc gtaaagcgga ttgtgtcgcg atcgcaatcg
     7441 agctctgcgc ggataagggt ttggtcgttt ccagatgact cccccttgac ccaaaggccc
     7501 cttgagcgag aatgatacgt tcctttgccg ctattcaatg ctacttttag actctccatg
     7561 ctcgaatgaa caagcccaag tgcctttcca gactcatcgc agacaagcgt aggccatagt
     7621 ccatcggccc tatcggtaac aagaggtgcg gcaaaggcct ccgcaagaga taaatcgccc
     7681 aacacaagcg aagtgccaac ttggagatcg gcacccagtc tatccaaggc cgcaatatct
     7741 gcaatgcctc gttcgcctcc ggcggcacct ccagcaaatg tgaattttct atcgggcgag
     7801 agagcaatca gctttcttgc tctttcgata tcgattccgc actggtcgcc ctcggtttcg
     7861 atgaaggtga tgagaaaccc agacacatag ggggaaagaa cactgatcga ttgttcaatg
     7921 gaaccttccc ttggcttcga ccatccttcg accatgatag tcccttcctt gctgtccaag
     7981 gccgcaataa gtcgatcacg cggtaagctg gaaagaaatt cgggtgtggc cgaagttcct
     8041 atcattatcg aacgggctcc ttcattgagg taatccatag caaggccaat cgttcgaatg
     8101 cctccaccta cgcgtactcg atgggttctg catagagaaa gaatgagctc tcggttgctt
     8161 ccgattcctt tggcagcatc gaggtctact acggcgaact ctccaaccct agcatatcgt
     8221 tcggcgagct ctagagggtt tccagcatcc agaggtggct gctttcctcc tctaagctgt
     8281 actgctctgc cattcataat atcgatacta ggcacgatca taatcttacc tccacatcgt
     8341 gtgctttcaa gaattctttt atctctcgaa tcgagacttc accgcgatgg aaaatcccag
     8401 cggcgagcag agcggatgcg ccacttaaat atccctccag aaaatgctct agcattgaag
     8461 cacctccaga agcgataatc ggtacagata ctatacgggc gacaatttcg ataagtctgc
     8521 aatcgtaacc caggcaagtg ccatcgcgat ctatcgaagt gagtagaatc tgtcctgcac
     8581 ctcgctctgc ggcttcttgt gcccattctg atacatcgat acccatcgat ttggagcctc
     8641 catcgatttt cacttcatat cccgatggca tagatgcatt atgtgcagca tcgatggcta
     8701 ccactatgca ttctgttccg aatattgagg ccatttcatc gattatcgca ggttttttca
     8761 cagcaacgct gtttatcgag acatagtccg cgcctgcctc gaacaatcgg gctgcatcct
     8821 cgatgctcgc aattccgccg cctacagtga gaggaatcga aatcgcctgg tgtatgcgcg
     8881 caacttcctg cgccatggtc tttcttccct ctatagtggc gctgatatcg agaaaagcaa
     8941 gctcgtcagc tccttgcttt tcatagataa gagcgcgctc tagaggatct ccagaatcta
     9001 cgagattttg aaatcgcttg cctttgacaa ctcgaccttc tttgaaatcg aggcatggaa
     9061 ttatcttgac agcagacatt ctcatgcatt tttgcaatgc tgacttagat ttcttgttcg
     9121 tattcatgtt tgctccgaat ctatgcatgt ttcccccttt gtgcttgcta aagccgcgct
     9181 tggtgcgagc gcagctctga atgcaagcgc aactgcctta aaaagcgctt cagccctgtg
     9241 gtggtcattt tctccacgaa gaatgtcgag gtgaagcgta catcgtgcat tcgttgcaaa
     9301 gctctgaaaa aagtgctcaa tattgcaggt cgaaagcgag cctatagatg gtgtggagaa
     9361 gtctccggag aacacaaagt agcttctgcc ggaaatatcc agcgcagcac gtgcgagagc
     9421 ctcgtccatc ggggcatagg aatagccaaa gcgcgcaagg cctttcgccg aagggacgat
     9481 tttggagaat gctattccaa gggcaatgcc tacatcttct actgtgtgat ggtcatcgat
     9541 atccagatct cctttgcatg aaattttaag gctccagcca gcatggaatg caagctgagt
     9601 aagcatatgg tcaaaaaagc ctatgcctgt attgatatcg atagatcccg gcgataattg
     9661 taaactcacg ccgacatctg tctcggctgt tgttcttctt atggttgcta ttggttgatc
     9721 attcatagac gctccttgtt cttaggtttg ctctttattc tgttattttt atcatattct
     9781 ctcactgcat cttcgagaga accacatagt tcctcgaatt ctccttggtc tcctggaacg
     9841 gtgattctta ccagctcttc atatcctggt tttctaggcc aggtacgcac aagaattcct
     9901 cgttcgcgca atttatcgga tagatcagta ggattttcgc ttttcaagag aatgaagttt
     9961 gcttcgctct ccgatactaa aaagccaagt tttctgcaaa gcgatttcag ggtctgcact
    10021 tcccgccgga cctcatcaat aaagccttga gcaactcctt gatcaagaga aagcgctctt
    10081 ctgccggctt caattgccga gctcgaaagc gagtaaggag ggccagcttc cgaaagtctc
    10141 gagatcaact gcgtaaactc aggattagcc gcaacatacc cgattcgaaa acccgcaagg
    10201 cccttggatt tggaaaagct accggttaca agcacatttg ggttggctag cgcctggata
    10261 ggcgttgatc catgtatcga aaattcctga taggttgcat cgaagacaaa gatagtgcca
    10321 agctcctgac agaccgtggc gatctgtgtg acatcgaggg gggagagaac aactccagaa
    10381 ggattatcgg ggctcgacag aataaggagt cttggagtca gggccctgac gcatctgcat
    10441 atatcgtcga tcggaaaagg ttcaaatgga tcctttttta tttccttgaa acgagctgat
    10501 gatcgctggg cggcttccaa gaattcgaca aaaccaggac tcgtactcat gataagtcct
    10561 cctggccctg caagactccg tattgttcta tcaatagcat catccgctcc agcggttgca
    10621 agcacgcagg atgaaggcag cccgagcttc tcggctaaag gtatttccaa gagctccctc
    10681 atagggtagc gccgggctat ttcagggctc aatatctctc taagatcttc ttctgaaaga
    10741 atgcatcggc cttcattggc atctagtcta agcattgtct cctccttttg gcttatggca
    10801 caagctgcga tatctgagtt gctacaatat cggtgccccc acggcgcttt atctctggga
    10861 taaggcttgc aagcatatcg cgaggagctg caactttaac agcaaaggca tctcctcctc
    10921 ccagagggga tacggtgggt gctcttagac agggcagaat agccacaagc tcatcgagaa
    10981 gttcagccga tatattgact tcaagcatca cacgacgcct cgcttcgagc acagaacgga
    11041 agagaagaac aagcgcttcc gcagcttcgc gcttgtcttc atctgctatt gcagttttcg
    11101 aggcatagaa tcgagtcgag cttgtcatca gggtatctag tatttcgagt ctatttgccc
    11161 tcagggtaga gcctgttgcg tagttgtcga ttatgatgtc cgcatcttcc ggagggaaaa
    11221 cctcggttgc tccataagaa tgtacaaatt ctgcgccagg taggtttata tcgatccagg
    11281 cctttgcaag tccttcatac tcggaagcga cgataggggt ccgtttgcag gcagctatca
    11341 tctttgtcag gtctcctctg gaggaggcaa aggctgtcgc tacattttcg ggagccgcgg
    11401 cgacgatctt gactggatcg agaccggtat ccagaagctc gatcaaatca gctccaagtt
    11461 cgcaaaccca atccgcgcct gcaaaaccca tatctcggct tccagcctga agcatctcga
    11521 caatgttttg gggttttagg agttttgctt cgtataccat ttctcgagat ccaaagacta
    11581 cggcttttcc atttccgatt cgtggacgat attctcgttc atcgatagat acaggtatgc
    11641 ctgcatcatt gaaaagctcg attacctttg cctgcatcct ccctttagga agaccgagcc
    11701 tcaattcaac cttttcattt gcgctttctg tcattttctt ctccttttcc tcgtagcctt
    11761 ttctcagcta agaaaaaaag aagccgaccc tcttaggagc cggctttggc aagatcaaaa
    11821 tcaatgaaaa agccggcccc ctggagccgg cttcggtttc gt
//
LOCUS       DAOSOB010000057        11814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_5384_length_11814_cov_76.706608, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000057 DAOSOB010000000
VERSION     DAOSOB010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11814
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5384_length_11814_cov_76.706608"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PLS88_06750"
     CDS             <1..797
                     /locus_tag="PLS88_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOU07077.1"
                     /translation="VNRIAGDRAEVIVIMPPGSNPHSFEPSPRQLAELGTAKLWFSIN
                     VDFEYSFKPKLRSMFPKLQIVDVTKNVQFRRLRPTEQEHDEEAAEGHSENIDPALQNR
                     DQHTWLGIEQAKAEIKVIQDSFIAIDPEGRELYKSNCQNYLKDIDIVYSTLKTKLAPL
                     SGAKVFVYHPAFGYFLDMFDIEQVAVELGGKEPTQKELYALIDLAKKEKARAVFTQAQ
                     FSESAAKAIASAIGAAVMPIDPLAADWLNNLSRIGQALSTAIAKGE"
     gene            799..1689
                     /locus_tag="PLS88_06755"
     CDS             799..1689
                     /locus_tag="PLS88_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU07078.1"
                     /translation="MNQAAQNEAATLGQTPSSLDSMDGKEHGVIVEPALPHPPENVAI
                     WCHDLSFRYREVEVFRNVTFHVHSGEFVVLTGKNGAGKSTLLKLVVGLLEPQQGQVLV
                     FGVSPRRRIESIGYVPQHASFDPAFPIRVEDVVGMGRLSGLSYISKDQKRGDIEWAME
                     QAEVTDLRARPFSALSGGQRRRVLVARALVGHPRLLVLDEPTANMDIDSENKFFEVLA
                     RIKMGATILIATHDSDFVSELADTVLCVGQDSEHPHSVHRHAMEKAEDLPKDIYGSNM
                     LRVRHDIELPDDACCEGKDP"
     gene            1686..2543
                     /locus_tag="PLS88_06760"
     CDS             1686..2543
                     /locus_tag="PLS88_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOU07079.1"
                     /translation="MISFFQAVMNPSVPFVRNALIGSVLAAFLFGILGSMVTVRRIAG
                     LAGAISHTVLGGIGLALFLVSRGAPGWISPMLGALVFAILAAITISIVSIKAKQREDT
                     VINALWAIGMSLGVIFMAKTPGYADPMSYLFGNILLVTQKNLYILGALNIIVLLLVVR
                     FYRHIEAASFDEEFAATKGLPVMAIYLFLLLIIAVAIVMLQTFVGIVMVIAMLTLPAG
                     TAGFFSHNLAEMMVNSSILALIFSVLGLILSWVFDLPAGALIVLIAGMVYIGISVGRM
                     LQGRRSSKS"
     gene            2637..3017
                     /locus_tag="PLS88_06765"
     CDS             2637..3017
                     /locus_tag="PLS88_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOU07080.1"
                     /translation="MTKARNHVLDSIIQATEPLSARQLCDKYKSEHDPATIYRALHFL
                     EEKGRIDSFILYCNAHGTERYYVLHRSEHRHWFHCERCHRFTDLGQCQFDEIVAQMSA
                     EKGLSITSHSFYAIGICGECRKHE"
     gene            3085..>3454
                     /locus_tag="PLS88_06770"
     CDS             3085..>3454
                     /locus_tag="PLS88_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07081.1"
                     /translation="MSKEKPILIEDLDLSLLANIKEKDLTAQGLMVLEGRIVIEKALE
                     SGIVPRFLVCTEAQEEYWQKRTKEIYAIQDNGAVSGDDVRESVFPMHIVSHDSVCNLV
                     DFRFHRGAIAVAEIPQIKPFD"
     assembly_gap    3455..3554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3555..4086
                     /locus_tag="PLS88_06775"
     CDS             <3555..4086
                     /locus_tag="PLS88_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU07082.1"
                     /translation="SSRGIEMPESSRCRLYLCLWNVTDPSNLGALIRTAVCLGAAAIL
                     LGPGCANPYYRKTIRSSMGNVFSIPICSVDLNTLETLDRQSAKVIAAALTEKAIKPQE
                     LKFAFSRQTSENSPALDYCSNSVILLLGNEGYGLPTEVLSLTDIQVCIPMAAGSDSLN
                     VAVAGGILMYEIVRSF"
     gene            complement(4193..6553)
                     /locus_tag="PLS88_06780"
     CDS             complement(4193..6553)
                     /locus_tag="PLS88_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOU07083.1"
                     /translation="MNEKIRHLREESFNAKPSFSAERALLTTEFYKEWYGRVSMPVLR
                     AMNFYNLCEKKTIYIGPDELIVGERGPKPKAVSTFPELTCHSEEDLRILDSRQMTSYS
                     VSAEDMEIYKTKVIPYWRGRSLRDRAFAEIPNEWKDLYEAGLFTEFMEQRAPGHTALD
                     GSIYSKGMNARLIEIAATRAALDWDKDPDVLAKDEELKAMDIACRAAILFAERHATLA
                     DSMAGSESNPRRATELRQIAEVCRRVPAEAPRNFWEAIQMYWFVHLGTITELNGWDAM
                     SPGHFDQHLSPFYEQDLLNGMLDREKAKELLSCFWIKVNNTPAPPKVGVTAAESGTYN
                     DFTNINLGGLRPDGSDGSNEVSYLALEVLDELQLLQPQANLQVSAKTPERLLKTACKV
                     ARRGSGYPSFFNADEVIMAQLGMGKRIEDAREGGISGCIETGCFGKEAYLLHGYLNSP
                     KILELVLNNGIDPLSGKAIGLRTGDPSSFQTFEELYAAFERQLKYVVDIKVRVSNYLD
                     RMFAEYAPAPFLSSVVADCIAKGKDYYNGGARYNTDYIQCCGLGTMTDSLSAIKTHIY
                     EKKDINWNELLSALSSDWQENESLRLLMANKTPRFGNDDEAADSIARRVFNSWFGAID
                     GRASPRGGTYHIDLLSTTCHVYFGLKTGASPDGRHAHSPISDGASPAQGADRNGPTAV
                     IKSLAKIDSAKTGGCLLNQRFLPQTLEGEKGIDNLTSLIKTFFRLGGHHIQFNVVDTK
                     TLREAQKKPENYRNLLVRVAGYSDYFVDLDRNHQEEIISRTAQQAL"
     gene            complement(6574..7605)
                     /locus_tag="PLS88_06785"
     CDS             complement(6574..7605)
                     /locus_tag="PLS88_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOU07084.1"
                     /translation="MSKVEEDNMTSGIVFDIRRFSVHDGPGIRTAVFLKGCPLHCLWC
                     HNPEGISFSPEILRRTERCVDCRTCVRLCPLKIDPRAEAGGHLCKDCPSFGCCAEACP
                     AEALQIIGRRITVSVLMEAIRADLPFYEESGGVTFSGGEPLAQKEFILALLRACKSEG
                     IHSALDTSGFTDRDFMLEAGMLTDIVLFDLKLLDEEKHRKFTGVSNTSILANLSALYE
                     AGANVVLRLPLIPGINDAPEELEKMALYIGSLEAKPTELRPIEIRHTDKESIETGPIS
                     DNSWPSAQTKFLPKKAHILPYHDSAKDKYRLRRQEYAMSGTAIPSTESIDRAVSIFEA
                     AGISVTIGG"
     gene            complement(7598..>8253)
                     /locus_tag="PLS88_06790"
     CDS             complement(7598..>8253)
                     /locus_tag="PLS88_06790"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU07085.1"
                     /translation="CAINSESLASSVYEELKRRLNAGMLKPGQFIDLTALGNELGISR
                     TPLRDALIRLELEGFIVIYQRRGVMVKVLQLSDIRDIYQIIGALEASAIEEISKRFRA
                     SDADKMDSLCVEMADALSADDFDRYYRANLKFHDVYLELSENKKLIDNVHILKERLYD
                     FPRRSSYIKEWELDSMAEHAEIVKRLRSADFKGAASYVKDVHWSFEVQERYIRKYYV"
     assembly_gap    8254..8353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8354..9844
                     /gene="glpK"
                     /locus_tag="PLS88_06795"
     CDS             8354..9844
                     /gene="glpK"
                     /locus_tag="PLS88_06795"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOU07086.1"
                     /translation="MDRNSTYILALDQGTTTSRAILFNHDGIIISVKQIPFRQIFPKP
                     GWVEHDPEEIWRTQLKAARGAIEAAQIEPTQIAAIGITNQRETTIVWERATGKPVHNA
                     IVWQCRRSSSICDELKAQGATDLIRQKTGLVLDPYFSGTKLTWLFREHPEIKARAERG
                     ELMFGTVDSWLIYNLTGVHATDPSNASRTMLFDINERRWDKELLSLLEVPADILPEVR
                     PSSGLFGVAKKDIFGAEIPVAGVAGDQQAALFGHACFEPGDVKNTYGTGCFTLMNTGP
                     FPVESKHNLLATVAWDLGKGYTYALEGSVFIAGAVIQWLRDQMGLLTDSAESERFARM
                     VEDSQGVYIVPAFVGLGAPYWDSEVRGTVVGITRGTSKAHFIRAALESIAYQSMDLFE
                     AMEKDSGRRLSCIKADGGASQNSFLMQFQADITGTRVVLPEVSETTALGAAYLAGLAI
                     GYWNSLDDVRKNWRMRREFSPRMAEESRAGLVEGWHKAVATARNFR"
     gene            9975..10436
                     /locus_tag="PLS88_06800"
     CDS             9975..10436
                     /locus_tag="PLS88_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOU07087.1"
                     /translation="MERTDEAVALHVAGSACSQAVFTVFAEELGLEPSVAHRISGGFG
                     GGIGRMGLTCGAVTGGIMALSLALGAADSADQDSKLELYQIVAEYIERLKAFKGSIEC
                     RVLLEGADLWTTEGRDTVKARQLSEKVCNPMIREAVEEAERILSEKGIFKG"
     gene            10450..11277
                     /locus_tag="PLS88_06805"
     CDS             10450..11277
                     /locus_tag="PLS88_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOU07088.1"
                     /translation="MQYERFEGSWFLKSNWHLVKTGQSDQSKGVPVPPQEEPPAPEDC
                     VIDLPPPHRILKSYGENQRQKSESRSDMDMKSREKVIPELSERAAVLYHLLNNRKSRR
                     KYTEEPLAIEELSYLLWAIEGVKENRGKFSFRTTPSGGARHPLDVYIFAHKVEGLNVG
                     LYRYLPVEHELVLERQGDDSVALDEALNGQFWNAACVVMWAAVPYRSEWRYGKAAYKL
                     VALDTGHSCQNLYLACESLGLGTCAIGAYDQERLDAYLGLDGEEMFAIYAAPVGHVV"
     gene            11423..>11814
                     /locus_tag="PLS88_06810"
     CDS             11423..>11814
                     /locus_tag="PLS88_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOU07089.1"
                     /translation="MTIDTVKLDFPTNCNIIIGQSHFIKTVEDIAEIMMNSVPGVKFG
                     LAFCEASGPCLVRTEGTDPYLIEVAQKMAMAVRAGHSFYLVIGPGAYPLNVLDRIKAN
                     PETCRIFCATANPVEVVRAIIDQGAAILG"
BASE COUNT         2994 a         2676 c         2871 g         3073 t
ORIGIN      
        1 gggtgaaccg tatcgcgggg gacagagctg aagtcatcgt cataatgcct ccaggttcga
       61 atcctcattc ttttgaaccc tcgcctcgcc agcttgccga attaggaaca gcaaagctat
      121 ggttttccat caacgtagac tttgagtatt cttttaagcc gaaattgcgc tctatgtttc
      181 caaagcttca aatagtcgat gtaacgaaga atgtccagtt ccgaaggctc cggcctactg
      241 aacaggagca tgatgaagaa gcggctgaag gacactctga aaatatagat ccggctctcc
      301 agaaccgaga tcagcataca tggcttggca tcgaacaggc caaggcagag ataaaggtaa
      361 tccaagacag ctttatagca atagaccctg aagggagaga actatacaaa agcaattgtc
      421 aaaattatct aaaagatata gatatagtat actcaacatt gaagacaaag ctggcaccgc
      481 tttctggcgc taaagtattt gtatatcacc ctgcatttgg ctatttcctt gacatgttcg
      541 atatcgaaca ggttgcagtc gagctaggcg gcaaggaacc gacacagaaa gagctttacg
      601 ctctcataga tcttgcaaaa aaagaaaaag cccgcgcagt ttttactcag gcccaattct
      661 ccgaaagcgc tgcaaaagcc atcgcaagcg caataggagc tgcggtaatg ccgattgatc
      721 ctctggccgc tgattggctg aataatttgt ccagaatagg ccaggctctg tctacggcaa
      781 tagccaaagg agaatagaat gaaccaagca gctcaaaatg aggctgctac gttagggcaa
      841 accccaagca gcctagattc tatggatggc aaggagcatg gggtaattgt tgaacctgct
      901 ctgcctcatc ctccagagaa tgttgctatc tggtgccatg acctgagctt tagatatcgc
      961 gaggtagagg tattcagaaa tgtcaccttt catgtgcata gcggcgaatt cgtcgtgctc
     1021 accggcaaga atggagcggg caagagcact ttgctcaagc tcgttgtagg tcttctggaa
     1081 ccacaacagg gacaagtttt ggtatttggt gtctctcctc gcagacgaat tgaatctata
     1141 ggctatgtgc cccagcatgc aagctttgac ccggcttttc ccattcgagt cgaagatgtc
     1201 gtaggcatgg ggaggctttc tggactttca tacatttcta aggaccaaaa aaggggagat
     1261 atcgagtggg ctatggagca agcagaagta acagatcttc gagcgcgccc attctcggct
     1321 ctttctggtg gacagaggcg aagagtcctg gtagctcggg cgctggtagg acatccaagg
     1381 ctccttgtgc tggatgaacc aacggcaaat atggatattg acagcgagaa taaattcttc
     1441 gaagtactgg ctcgcataaa aatgggcgca acgatcctaa tagctaccca tgattcagat
     1501 tttgtgtcag aactggcgga tactgttcta tgtgtaggcc aggattctga gcatcctcac
     1561 tctgtacata gacatgccat ggaaaaagca gaagacctgc caaaagatat ctatggaagc
     1621 aatatgctac gagttcggca cgacatcgag ctacccgacg atgcttgctg cgaaggaaaa
     1681 gacccatgat tagctttttc caagcggtaa tgaatccttc ggttcccttt gttagaaatg
     1741 ctcttatagg cagcgtgtta gcggcctttt tgtttgggat tcttggctct atggtcactg
     1801 tgcggcggat tgctgggctg gctggcgcga tttcgcatac tgtattggga ggcatagggc
     1861 ttgctctctt tcttgtttcg agaggtgctc ctgggtggat aagccccatg ctcggtgcgc
     1921 tcgtgtttgc catccttgcg gctataacga tcagcattgt gtcgataaag gcaaaacagc
     1981 gcgaagacac ggtgataaat gcactttggg ccataggcat gagccttggt gtgattttta
     2041 tggccaagac tccaggctat gcggatccca tgagttatct cttcggcaat attctccttg
     2101 tgactcaaaa aaatctttat attttaggag cgctgaacat aatagtattg ttgcttgtag
     2161 tgcgattcta cagacatatc gaagcagcat cttttgatga ggaatttgca gctaccaaag
     2221 gtctgccggt tatggccatt tacctttttt tgcttcttat tatcgcggtt gcgatagtta
     2281 tgcttcaaac ctttgtgggt atagtcatgg tgatagcaat gctaaccctg cctgctggca
     2341 cggctggctt tttttcgcac aatctagctg agatgatggt gaattcgagc attctggcgc
     2401 ttatattttc ggtgcttggg ctgattctgt catgggtttt cgatttgcca gcaggagcat
     2461 tgatagttct aatagcgggg atggtctata ttgggatttc ggtgggcaga atgctccaag
     2521 gacgtaggtc gtcgaaatca tgattctcgg agtcgagatg agcaatagaa aatcacgagc
     2581 tcggcaagga aatcgagtaa agagctcgag ctaggagctc gggccaggag aaggcaatga
     2641 caaaggcgcg taatcatgtt ctagacagca ttattcaagc aacagagcca ctctctgcgc
     2701 gtcaactctg cgataaatat aaaagcgagc atgatcctgc cacaatctat cgagcgcttc
     2761 attttctcga agaaaaagga cgcatcgatt cttttatttt atactgcaat gcgcatggaa
     2821 cggagcgtta ttatgtgctt caccggagcg aacatcgaca ttggtttcac tgtgagcgct
     2881 gtcatcgctt taccgacctt ggccagtgtc aattcgatga aattgttgca cagatgagcg
     2941 ctgaaaaagg actttcgatc acttcgcact cattttatgc cattggtata tgtggcgagt
     3001 gccgtaaaca tgaatgatgg gccttttttc ttattgctgc atacctttta tatatgagca
     3061 ttaaagaagg agatttgaca aaagatgtcg aaagagaaac ctattctcat cgaggatctt
     3121 gatcttagcc tcctcgcaaa tataaaagaa aaagacctaa cagctcaggg actcatggtt
     3181 ctcgaagggc gcattgtcat cgaaaaagcc ttagaatctg gtatcgtacc gcgtttcttg
     3241 gtttgcaccg aggcgcaaga agaatactgg caaaaaagaa cgaaagagat ttatgcaatc
     3301 caagataacg gcgcggtcag cggtgatgat gttagggaaa gtgttttccc aatgcatatc
     3361 gtgagccatg attcagtctg caatcttgta gattttagat ttcatcgagg agcgatagct
     3421 gttgcggaaa ttccacaaat aaagccattt gaccnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnaagttc tagaggaata gaaatgccag aatcctcaag atgcaggcta
     3601 tatctctgcc tctggaacgt caccgatcct tcgaatctgg gcgctctcat ccgaacggca
     3661 gtttgccttg gtgctgcggc tatccttctg ggtccaggct gcgccaaccc ttattaccga
     3721 aaaactatcc gctcatctat gggaaatgta ttttctattc caatctgcag cgtagatttg
     3781 aataccttgg aaacactaga tcgacaaagt gcaaaggtaa tagcagcagc cttgacagaa
     3841 aaagccataa aaccacaaga gctgaaattt gctttttcgc gccagaccag cgagaattca
     3901 cccgcactag actattgctc caattctgtt atccttttat tgggcaacga gggctatgga
     3961 cttccgacag aagtgttgtc tcttacggat atccaggtgt gcatcccaat ggctgctggc
     4021 agcgattctc tcaatgtcgc cgtagcagga ggaatactaa tgtatgagat tgttcgatcg
     4081 ttttgactct tatgctaatt ttagggtatt aaatgagtct tgaaagttct acttatttgt
     4141 ggccccctaa ttatctgaaa taagaatagg ggcagagcaa agccaagcag ggtcagagtg
     4201 cctgctgcgc cgtccgactt atgatttctt cctggtggtt tcgatcgagg tcgacgaaat
     4261 aatccgaata tccggctaca cgcacaagaa ggttgcggta gttttcgggc tttttttgag
     4321 cctctcgcag cgtttttgta tcgacgacat tgaattgaat atgatgacca cctagtcgga
     4381 aaaaagtctt tattagactt gtcagattat cgataccttt ttctccttct agcgtctgag
     4441 gaagaaaacg ctggtttaga agacaaccac cagtttttgc cgaatctatt tttgcaaggc
     4501 ttttgatcac cgcggttggc ccatttcggt cagctccctg tgctggagat gctccgtcgg
     4561 atattggaga atgcgcatgt cttccgtcag ggcttgctcc ggtctttagt ccaaagtata
     4621 catggcaggt tgtggataat aggtcgatgt gataggtgcc gcccctaggg gaagctcttc
     4681 catcgatcgc cccgaaccag ctattaaaaa cacgccgagc aatggagtcg gctgcttcat
     4741 catcgtttcc gaagcgtggc gttttgttcg ccataaggag ccttagactc tcattttctt
     4801 gccaatccga ggatagagcg ctgagaagct cattccaatt tatatctttc ttttcatata
     4861 tgtgagtctt aatggcagat agcgagtcgg tcattgtccc gagtccgcag cattgtatat
     4921 agtccgtatt ataccgcgct ccgccattgt aataatcctt tcccttggcg atacagtcgg
     4981 ctaccacaga agacaagaaa ggcgctggcg catactctgc gaacatgcgg tctagataat
     5041 tcgaaaccct tacttttata tcgacgacat atttcagctg gcgttcaaag gcagcatata
     5101 actcctcgaa ggtctggaaa ctggatggat cccctgtcct aagcccaatc gccttgccgc
     5161 ttaatggatc gatgccattg ttgagaacga gctctagtat ctttggagaa ttgagatatc
     5221 catggagaag ataagcctct ttgccaaagc aaccggtctc gatgcaacca gaaataccgc
     5281 cttcacgagc atcttcgatt cttttgccca ttccaagttg agccattatg acttcgtccg
     5341 cattgaaaaa cgaaggatag cccgatccgc ggcgagcgac tttgcatgca gtctttaaga
     5401 gccgctccgg cgtttttgcg cttacctgaa gattggcttg tggttgcaga agctgtagct
     5461 catccagcac ctcgagcgca agataagata cctcgttaga tccatccgag ccatcaggcc
     5521 ttagaccccc aagatttatg ttagtgaaat cattgtatgt tccgctttcg gcagccgtaa
     5581 caccaacttt aggcggcgcc ggcgtgttgt tcaccttgat ccagaaacaa gacaggagct
     5641 ctttagcctt ttccctatcg agcattccgt tcaggaggtc ttgctcatag aatggcgata
     5701 gatgctgatc gaagtgcccc ggactcattg cgtcccagcc atttagttcg gtaatggtgc
     5761 caaggtgtac aaaccaatac atctgtatag cttcccagaa attacgagga gcctctgcgg
     5821 gcactctgcg acagacctcg gcaatctgcc taagctcggt tgcgcgcctt ggattggatt
     5881 cagagccggc catggaatct gcaagcgtcg catgacgctc ggcaaagagg attgcagctc
     5941 tgcacgcgat atccatagcc ttgagttctt catctttagc cagaacatcg gggtctttgt
     6001 cccagtccaa ggctgccctt gtggcggcaa tctctatgag cctagcattc atgcctttgc
     6061 tgtatataga gccatctagg gcagtatggc ccggagctct ctgctccatg aattcggtga
     6121 atagccctgc ctcatagaga tccttccatt catttggtat ctccgcaaat gctctatcgc
     6181 gaaggcttct gcctctccag taaggaatca cctttgtttt gtagatttcc atatcctctg
     6241 cggaaaccga gtagcttgtc atctgtctgg aatcgagaat tcgcaggtcc tcttcggaat
     6301 ggcaggtcaa ttctggaaag gtagatacgg ctttgggctt gggccctcgc tctcctacta
     6361 tgagctcatc cggaccaata tagatagtct tcttttcgca tagattatag aagttcattg
     6421 ccctgagcac aggcatcgaa acacggccat accattcctt atagaactcg gtagtcaata
     6481 atgctcgctc agccgaaaaa cttggtttgg cattgaaact ctcttctcta agatgtctta
     6541 ttttttcgtt catagctacc tccaatagga ttgctaacca cctatcgtca ctgaaattcc
     6601 tgcggcttcg aatatcgaga cagccctgtc gatagattca gtagaaggaa tagctgtccc
     6661 gctcatcgcg tattcttgcc gtcgcaacct atatttatct tttgcagaat cgtggtaggg
     6721 cagtatatgc gccttttttg gcaagaactt tgtctgtgcc gaaggccaag aattatccga
     6781 gataggtccc gtctctatgg actctttatc cgtatgcctt atttctattg gcctcagctc
     6841 tgtgggcttt gcctcgagag aaccaatgta gagagccatc ttttctagct cttccggtgc
     6901 atcgtttatt cctggaataa gcggaaggcg cagtactaca ttagcccctg cctcgtagag
     6961 tgcggatagg tttgccaaaa tgctcgtatt cgagacacct gtaaattttc tatgcttttc
     7021 ctcgtctagg agcttcaaat cgaagagaac gatatcagtg agcattccgg cttccagcat
     7081 aaaatcccta tcggtaaagc ctgaagtatc gagagcgcta tgtatccctt cggatttgca
     7141 agcccttaga agcgcaagga tgaattcttt ttgcgccaag ggctctcctc ccgagaaggt
     7201 aactcctccc gactcttcat agaaaggcag atctgctcgt atagcctcca tcaaaacact
     7261 aaccgtaatg cgccttccta ttatttggag cgcttcggca gggcaggctt ctgcacagca
     7321 tccgaaagaa ggacaatctt tgcataaatg gcctccagcc tcggcccttg gatcgatctt
     7381 taaaggacaa agccttacgc atgttcggca atctacacag cgctccgttc ggcgcaagat
     7441 ttccggagaa aaagaaattc cttcaggatt gtggcaccag aggcagtgaa gcgggcatcc
     7501 ctttagaaac accgccgtcc gtatccctgg gccatcgtgg acggaaaacc tgcgtatatc
     7561 gaatacaatc ccagaggtca tattatcttc ctcgacttta gacataatac ttcctgatat
     7621 agcgttcctg cacctcgaaa ctccagtgca catccttcac atagctcgct gctcccttga
     7681 agtcggcgct tcgcagtcgt ttgactattt ccgcatgttc ggccatcgaa tcgagttccc
     7741 attcttttat ataagatgag cggcgcggaa agtcgtaaag cctttctttg aggatatgca
     7801 cattatcgat tagtttctta ttctcagaga gttcaagata gacatcatgg aattttaggt
     7861 ttgctctata atatctatcg aagtcatctg cactaagagc atctgccatc tcgacacata
     7921 gactatccat cttatctgca tctgaggctc tgaagcgttt ggatatctct tctattgcgg
     7981 aagcctcgag ggctccaatt atctgataaa tatctcgaat atccgaaagc tgaagaacct
     8041 ttaccattac accgcggcgc tgataaataa caataaagcc ctctagctcg agccgaatca
     8101 atgcatccct aagcggcgtt cggctaattc caagctcatt cccgagagca gtgagatcga
     8161 tgaattggcc aggcttcagc atacctgcat tgaggcggcg cttaagctct tcatatacag
     8221 aagaagcaag ggattcggag ttaattgcgc atgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnatggacc gcaattcaac ttatattctt gccctagatc agggcacaac
     8401 gacttcaagg gctattttgt tcaatcatga tggtatcatc atctcggtaa agcagattcc
     8461 attcaggcag atttttccaa agccaggatg ggtagaacat gaccccgagg agatatggcg
     8521 cacacagcta aaggcagcaa gaggcgcgat cgaggcggcg cagatagaac cgacacagat
     8581 tgccgcaata ggcattacga atcagcgcga aacaacaatt gtatgggagc gcgcaacagg
     8641 caagcctgtc cataatgcga ttgtttggca atgccgtcgc tcgagctcga tatgcgacga
     8701 attgaaagcg cagggtgcta ccgatttgat tcggcaaaaa acaggacttg tgctcgatcc
     8761 atatttctcc ggcacaaagc tcacatggct cttccgagag catcccgaaa taaaggcgcg
     8821 cgccgagcgg ggtgaattga tgttcggaac tgtcgattcc tggctcatct ataatctcac
     8881 gggggttcat gcgaccgatc cttccaacgc gagccgcaca atgctcttcg acataaacga
     8941 acgccgttgg gataaggagc ttttaagtct tcttgaagta cctgcggata ttctaccgga
     9001 agtcagacct tcttcgggcc tctttggtgt agcgaaaaag gatatatttg gtgcagaaat
     9061 tccagtggct ggagtagcag gcgatcagca agccgcactt tttggtcatg cgtgcttcga
     9121 gccaggagat gtgaagaaca cttatggtac gggctgcttc accttgatga atacaggacc
     9181 tttccccgtt gaatcaaagc ataatctgct cgcaacggtt gcatgggatc ttgggaaggg
     9241 ttacacatat gctctcgaag gctctgtttt tatagccggc gcggttatcc agtggctaag
     9301 agatcagatg ggtctattga ctgactctgc ggagagcgaa agatttgctc gtatggtcga
     9361 agatagtcaa ggagtctata ttgttccggc cttcgtgggc ttgggtgcgc catattggga
     9421 ttcggaagtt cgaggcacag tggttggcat tactagagga acatcgaaag cgcatttcat
     9481 tcgagccgcg ctggaatcca tcgcctatca atccatggac ctgtttgaag ccatggaaaa
     9541 ggattcggga agacggcttt cctgtataaa ggccgacgga ggagcttcgc aaaactcatt
     9601 tcttatgcaa ttccaagccg atataacagg gaccagagtt gtccttcctg aggtgagcga
     9661 gacgactgcg cttggagcag cctatcttgc cggtcttgct ataggctatt ggaatagcct
     9721 cgatgatgtg cgcaagaatt ggcgaatgcg cagagaattt tcgccgcgaa tggcggaaga
     9781 atcaagagct ggtcttgtgg aaggatggca taaggcggta gctacagcaa ggaatttcag
     9841 gtaatgaatg ccgttgcaaa ttttatagta aatgcgatga caaatttaat agcaaatgcc
     9901 actgtaaatt ttatgacaga tatcatgtca gatgccgata caaggcaaaa gtgaattata
     9961 atcggaggat gagtatggaa agaacggatg aagcagtagc gctacatgta gctggatcgg
    10021 cctgctctca agcagtgttt actgtatttg cagaggaact gggcttagag ccttccgtgg
    10081 ctcatcgtat ttctggggga tttggagggg gaatagggcg tatgggcttg acctgtggtg
    10141 ctgttacagg aggaatcatg gctctttcgc ttgctcttgg agccgctgat tcggccgatc
    10201 aggatagcaa gctagagctt tatcagatcg ttgcagaata tatagagagg cttaaggcct
    10261 tcaagggcag tatagaatgc agagtcttgc tggaaggggc ggatctttgg accacggaag
    10321 gtcgcgacac ggtaaaagcc agacagctct ccgaaaaagt ctgtaacccg atgatccgcg
    10381 aagcggtgga agaagcagaa agaatattga gcgaaaaggg tatttttaag ggttaatttg
    10441 gagtttgata tgcaatacga gcgttttgaa ggaagttggt ttctcaaatc caactggcat
    10501 ttagtaaaga cagggcaatc ggatcagtca aagggagtac ctgttcctcc gcaagaggag
    10561 ccacctgctc cagaagactg tgttatcgac ctaccgcctc ctcatcgcat tctgaagtca
    10621 tatggtgaaa accagagaca gaagagcgaa tcgcgctccg acatggacat gaaaagccgt
    10681 gaaaaagtta ttcctgagct gtctgagcgc gccgccgtgc tttaccacct cctcaataat
    10741 cgcaaatcgc ggcgcaaata cactgaagag cccctcgcaa tagaggagct ctcgtatctt
    10801 ctttgggcca tcgaaggcgt aaaagaaaat cgaggcaaat tctcttttcg caccactcct
    10861 tcaggcggcg cgcgccaccc gctcgatgtc tatatctttg ctcacaaggt cgagggcctc
    10921 aatgtaggcc tatatcgtta ccttccagtc gagcatgagc tcgtcctcga acgtcagggg
    10981 gacgattcgg ttgctctgga cgaagcgttg aatgggcaat tctggaatgc agcttgtgtg
    11041 gttatgtggg ctgccgttcc ctatagaagc gagtggcgct atggcaaggc cgcatacaaa
    11101 cttgtagctc tagatactgg tcattcatgc cagaacctct atctcgcctg cgaatcgctc
    11161 ggtcttggaa cctgtgcgat aggcgcttat gaccaggaaa gactcgatgc ctatcttggg
    11221 ctcgatggcg aagagatgtt tgccatctat gctgcgcctg tcgggcatgt ggtttaattc
    11281 taggaccaga actcttgtca tgaatcgcgg cgaattttgg cttttattgg aggaatgggc
    11341 taatagccga gttttgaaag aatgtctatt gacaatgaaa tagattcaat gagaaacatt
    11401 ggcataagga gggtttttga ttatgaccat cgatacagtc aagctagatt ttccgacgaa
    11461 ttgcaacatc ataataggac agtcgcattt tataaaaacc gtcgaggaca tcgcagagat
    11521 aatgatgaac tctgtgccgg gagtaaagtt cggtcttgcc ttttgcgagg cttctggccc
    11581 atgcctcgtt cgcacagaag gtacggatcc atatctgata gaggtagctc aaaagatggc
    11641 catggcggtt cgcgcaggtc atagttttta tcttgtcatt ggacctggtg cgtatccgct
    11701 caatgttctt gaccgtataa aggcaaaccc cgaaacctgc aggattttct gtgctacagc
    11761 caatcctgtc gaggttgtaa gggctattat agatcaagga gccgcaatcc tcgg
//
LOCUS       DAOSOB010000058        11783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_5406_length_11783_cov_74.565825, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000058 DAOSOB010000000
VERSION     DAOSOB010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11783
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5406_length_11783_cov_74.565825"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLS88_06815"
     CDS             complement(<1..351)
                     /locus_tag="PLS88_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07090.1"
                     /translation="MISPRSLVMYKTKPAIVISKDLDRILIKPIDGDERRVRIKDLTL
                     VHAGPIDSFPPIQQSIEVDEAIELLKDENPEGLSIISWNEFAELAWSKAEAQHIVIAW
                     YTLLENPAIEILDNG"
     gene            507..785
                     /locus_tag="PLS88_06820"
     CDS             507..785
                     /locus_tag="PLS88_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07091.1"
                     /translation="MTALLVGIGLIVVAVLMVIPGILGWWSDVLAFLRGALPVIALMI
                     GALAVFIGIADIKDKIEEKKEEEREKEEEKNKSKNSENEEKATEKKGD"
     gene            885..1313
                     /gene="rplM"
                     /locus_tag="PLS88_06825"
     CDS             885..1313
                     /gene="rplM"
                     /locus_tag="PLS88_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOU07092.1"
                     /translation="MKTAFFTAATADRNWYVIDAAGKPLGRVAAKVASILRGKNKPTF
                     TPSQETGDYVIIINADKVDITGRKRQNKTYYHHTGYPGGIKDISFEELIARNPVSPIE
                     IAIKGMLPKGPLGRKLFKNVKVYAGPDHPHIAQNPIAIDI"
     gene            1330..1728
                     /gene="rpsI"
                     /locus_tag="PLS88_06830"
     CDS             1330..1728
                     /gene="rpsI"
                     /locus_tag="PLS88_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOU07093.1"
                     /translation="MVKNINLGIGTGRRKCAVARVFLRDGSGKIFINEKDLKDYFAVE
                     GLIAQVRKPLLVTDSENKFDAYITVVGGGLSGQAGACSHGLARALAQIDPANHISLKA
                     NGLLTRDPRMVERKKYGQRGARRRFQFSKR"
     gene            1735..2448
                     /locus_tag="PLS88_06835"
     CDS             1735..2448
                     /locus_tag="PLS88_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOU07094.1"
                     /translation="MAMRIDSVRKKASGSLEVAVSGGLLFCFDEVDAQLLELSSNNSS
                     LDVYSLTEHSEGESIDEEMILLLSRLSTLHRARNDALLLISRAEQASRQLYQKLLKKG
                     YEPEIAHICIRWICSEHYVDDRRYVRMLFQSHCVNRGQGPTRMQALAWPRIGLFENPQ
                     TILADSMATIPESDFMEAIKKNVESLLRKPSTFSRLNLNTISERRSFLRSFLRRQGFP
                     YQSVETFIETLVLKENKND"
     gene            2532..3185
                     /locus_tag="PLS88_06840"
     CDS             2532..3185
                     /locus_tag="PLS88_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU07095.1"
                     /translation="MDDDILTIEEVAKYLRVSERTVYDWAQKGEIPSGKIGTVWRFKK
                     SEIERWVNERLSSNRPVSLISSVKIQNIISPDRIIFLNYPSKRDALIKLAENLSTAPQ
                     IKNKEELISEILRREELMSTAIGKGIAIPHVRLNSVTDLVVSIGISRCDIVDFQTLDD
                     VPVRLLFMIAAAYNQHAYYLQTLSFFSARLKIVELRDGLLDCKTTQEAYDLLIQQDR"
     gene            3270..6149
                     /locus_tag="PLS88_06845"
     CDS             3270..6149
                     /locus_tag="PLS88_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU07096.1"
                     /translation="MYLKSLEIFGFKSFADRIRIEFSKGISALVGPNGCGKSNIVDSI
                     KWVVGEQSAKSLRAETMEDVIFNGTETRRPLSVAEVAITITNDEGALPLDVPEIEVKR
                     RIYRSGENEYFINGKPARLKEIRELFWDTGIGKSAYSVMEQGRIDQVLSSKPEDRRYL
                     FEEAAGITKHRVRAHEAELKLAKTEEHLRQIEAIIREVKQSYENLRVQSDKTIKYRQL
                     KDRIFETELDLYLIRLRQHIREQDRVNDLFNQKKKDREALVQRIDSMSQSMSADLDIL
                     NKLEARLIEMQKLIYGLAVEKNGKEKQKALLTERLREIKSIIEQLEARDRAIASKIES
                     LQEEEGDKEAEFAGYKTRIREIEANIHTFDENISAAALLLKANEESIRHNIEESKEIA
                     QRTTLLHEKLDTITERIAELVDERLRKSESQIEQKQKLEAQIQQKIKEISISASSRAD
                     RLQDYTRIASSLSAQDRSRELEGLVDGLRALSSLSSDLERDFLQYIEIEPGFLDELVA
                     PEGVMTQKRRIDAEIFDQSERIKRIEAENQGLSLRNTDLAKKIEAYRKTLEEAKLEKA
                     KIQAQMEAAKEALSVLKREIAGQEAYHREVASELLDQQRRCEKLEEEIKLLQEELAEI
                     EHKGRNLTEEMSELDKSISRATEDLGDKRKESTELEQNLQSIQQELENLHTAKAQSET
                     EIRNLKDNFADLYSRDLLAFESRMYEIRSPVSEIKEKLVSLKASLSSLGSVNLMAPEE
                     FEPVKQRFEFLSGQYDDLVKARADLVRITNEIRTESAQLFVETYNKIKKNFHNIFRRL
                     FGGGRAELRLSDPDHVLESGIEIFAQPPGKKLENISLLSGGERTLTAIALLFATYMVK
                     PSPFCFLDEIDAALDEANIVQLVNLLREFGTMSQFIVITHNKKTVASADTLLGITMEE
                     SGVSKAIAIRVQNENGVVHPVYVPDQPFEEEEVDYEEGRQLEANR"
     gene            6146..7387
                     /locus_tag="PLS88_06850"
     CDS             6146..7387
                     /locus_tag="PLS88_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOU07097.1"
                     /translation="MNAEMLGERLFYLDPWLKEADVVVKATKPVVNRGSTILGVLLSE
                     TIFYPEGGGQPADLGFIEDKQVMDVQEIEGEIWHYIELQNEKEEEILKPGSAVHLSLD
                     WPRRLYHMQQHTGQHLLSAVLEEEYGIHTISFHLGTEYCTIDVSAKDPAELPLAQIET
                     EVEAWIERDAAVLIHYCPPEDISTFRLRKRPPINEAVIRVVEIQGYDCSPCGGTHLER
                     TGQVRALKVLSLERYKGHVRMYFASGAHAIELMSRAYEDNREAATLLGSSIGDISIRL
                     REILEKSASLEKRAKRCLVEYAEAEAKLASIHAGPSDIIQFTLEKEDADWGTMLCKAA
                     VSLGRPAIASCLLDSTIIIQVPAKARFPALSAVLSEIMLNLGGKGGGGEAFYRASFAS
                     ADMAKQFALKAKEVLEGFQAT"
     gene            complement(7446..8561)
                     /locus_tag="PLS88_06855"
     CDS             complement(7446..8561)
                     /locus_tag="PLS88_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOU07098.1"
                     /translation="MNNIDETGEFLKSLGFPLLNIHAMAKHHDFITANKRILVIGPMG
                     SGKTEFAARVWRDSRVAIKKKGAVRRLTSTANVDRRNVFVVRSALDKSRFPDYPDDAL
                     AYRGGYERCGNRIGAAKDSFGLEVLIAENPEVGTWIVDEAAFFDERVAYLTKDEGELR
                     GLCFIFPTLLLNFRREIFNQTARLLLETATDVVPLTAYCEHEECLRDSLYTYRYYLVD
                     GQECPALYFDPLIIIGGDRTKHDGREPNYCTRCDDHHYLPAKEYTFFTLKPFGELAAR
                     GNIAPLFAELAALQGNIEESRLYSSIRGRCAEEIEREMQMNSLKVPLIAERALVYLYA
                     EQNLLSEEQMRFFIQKLNLDKDYLSQRLADNRRPLAL"
     gene            complement(8583..10187)
                     /locus_tag="PLS88_06860"
     CDS             complement(8583..10187)
                     /locus_tag="PLS88_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU07099.1"
                     /translation="MNSRLFLVVSFLALAFLPLAAQMQETEFVTVYGSQLPELDPQKA
                     IFSNEAQIHTAIYEGLFNYDPNTLEPMRALAQNWERSVDKKLYRFTIRDNALWSDGSP
                     ITAEDFVRSWFRMLDINAEYATFFDIIEGAKQYRMGLDKNPEHVGIKAASPNTLEITL
                     VRPVAYFTRLLCHQSFSVIHPSMIDAGDWRNAIPYPVSGPYKPVSMTDTELILEKNEA
                     YWDSKNVSIPKLRMMFTDDDELATSLFNTGYVHWLDGPGNYDKVLLQTAIQISPMYST
                     HYWFFNCRSEPWNDKRVRRALALLLPWDSIRSSEFYMLPAKTLVLPLPGYSKTKGIEK
                     TDSTEAMKLLEESGYPKGQGLPEIIIAFADYERPRSIANTFKTEWEKALDVKVTLKPM
                     QPSSYYESIGNRSEASSFTIAHETWIGDFADPEAFLQMWTSDAPLNIAGYNDSVFMDF
                     MIQSYDASDEKRMSILAKAETELLQGAACLPIYHNFAASVIDIDFIQGWYQNALDIHP
                     YKYLHFGNPSISPNVAKVSSSYISLR"
     gene            10229..10765
                     /locus_tag="PLS88_06865"
     CDS             10229..10765
                     /locus_tag="PLS88_06865"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOU07100.1"
                     /translation="MGIEKEFIPERLIVGVLSSDTEAEIAALDAMIGLYGPLCFQSSK
                     ESFLWTKYYCPEMGENILRSYWAFRELIDPSKLSMIKRATDAIELRLAKNSCRLVNLD
                     PGILGTARFCLATTKDRSHRIPLSDGIYAELTLIFEHGEFKALPWTYPDWASEPVRKM
                     MSELRAGLLGDLRHLHLI"
     gene            complement(10804..11703)
                     /locus_tag="PLS88_06870"
     CDS             complement(10804..11703)
                     /locus_tag="PLS88_06870"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOU07101.1"
                     /translation="MANPIGVASGIFGYGEEYEELVNIDELGALYTKTVTLEPREGNP
                     APRLVETPMGLINSIGLANPGLECFLKEKLPLLRNYSCAIIANIAGSTEDEYAMLIDG
                     IEERLSLSEPLPKIGIDGYEINVSCPNVCEGGMSFGIDPVIVKRLTRSLRKRTDKPLI
                     VKLSPNVTDIAEIARAAEEGGADAVSCINTVLGMVIDTDRMKPAIAKGIGGLSGPAIR
                     PIGVAATYRVSRSVSIPVIGVGGIARASDAIQYFLAGAQAVQIGTALFSNPHAPQEIL
                     SGITDWMKRHGVLRVHDINSMLK"
BASE COUNT         3491 a         2438 c         2719 g         3135 t
ORIGIN      
        1 gccattatca agtatttcta tggcagggtt ttcaagaagc gtataccaag ctataacgat
       61 atgctgtgct tcggctttgc tccaagcaag ttctgcaaac tcattccaag atattattga
      121 gagaccttcg gggttttcat cttttaatag ctctatggct tcatctacct ctatgctttg
      181 ttgaataggt ggaaaagaat caattggacc agcatggaca agagtcagat ctttgattcg
      241 cactctgcgt tcatctccat ctattggctt gatcaaaatc ctatctaaat cttttgagat
      301 aacaatagcg ggttttgttt tatacatgac tagagaacga ggcgatatca tgtgatgttt
      361 ataatctcga ttatgccgat atacaagtgt gtatcaacgc tccattgcga ataatcgatg
      421 cttgtgttat attagaaaac aattgaggac atatcggtcc gctaaacaca aatgccaaaa
      481 agcttgtaca attaaggaga agagaaatga ctgcattatt agttgggatt ggactaatag
      541 tagtggcggt actcatggta attccaggga ttttaggatg gtggagtgat gtcctagctt
      601 ttcttagggg tgctttgcct gtaatagctt taatgattgg tgcccttgca gtttttattg
      661 gaatagcaga tatcaaagat aaaatagaag agaaaaaaga agaagagaga gaaaaagaag
      721 aagagaagaa caagtctaag aattctgaaa atgaagagaa agctaccgag aaaaaaggag
      781 actaaaagtt gttttttggc tcggatgctt gacagatttt tgaaactgtg ggaatatgca
      841 cccgcatttt taagtattcc tcaataaggt gaaggcatat ataaatgaag actgcatttt
      901 ttactgctgc aaccgccgat cgaaattggt atgtcatcga tgcagcaggt aagccacttg
      961 gtcgcgtggc agctaaggtt gcttcgattt tgagaggcaa aaacaagccg acatttactc
     1021 caagtcaaga gacaggggat tatgtcatta ttatcaatgc tgacaaagtc gatataacag
     1081 ggcgcaaacg tcagaataaa acatactatc accatactgg atatcctggt ggtattaagg
     1141 atattagctt tgaagagctt atcgcccgca atccagtatc tccaatagag attgcaatca
     1201 aaggcatgtt gcccaagggg cctcttggcc gcaagctgtt caagaatgta aaggtctatg
     1261 ctggtcccga tcatccgcat atagcacaga atccgattgc aatagatatt tgagcggaga
     1321 aggaaagaaa tggtaaagaa catcaatctt ggtattggca cgggccgcag gaaatgcgca
     1381 gtcgccagag ttttcctacg agatggctca ggtaagatat tcataaacga gaaagatctt
     1441 aaggattatt ttgcagttga aggtctaatt gcccaagtta gaaagccgct tcttgtaacc
     1501 gatagcgaaa ataagttcga tgcatatatt acagtagtag gtggtggttt atccgggcag
     1561 gcgggtgctt gttctcatgg ccttgcccgt gccctggctc agatcgatcc ggcaaatcat
     1621 atttcgctta aagccaatgg cctattgact cgcgatccgc gcatggtgga aagaaaaaaa
     1681 tacggccaac gcggcgctcg ccgaagattc cagttctcaa aacgctgacg acttatggcg
     1741 atgcggatcg attccgttcg caaaaaggcc tccggatcgc tcgaagtggc ggtttctgga
     1801 ggccttctgt tttgtttcga tgaagtggat gcacagcttc tagaactatc atcgaacaat
     1861 agttcgttgg atgtctattc tctgaccgaa cattcggaag gcgaatcaat agatgaagag
     1921 atgattctgc ttctgagccg ccttagtact ctgcatagag ctcgcaatga tgctcttttg
     1981 cttatttctc gagctgaaca agcttcaaga cagctatatc agaaacttct aaaaaaagga
     2041 tatgagccgg aaattgcgca tatctgtatt cgatggatat gctccgagca ttatgtggat
     2101 gaccgccgct atgttaggat gctttttcag tcgcactgtg taaatagagg acaggggcct
     2161 actagaatgc aagctttggc ctggcctcga attgggcttt tcgaaaaccc tcagacaata
     2221 ctggctgaca gcatggctac tattccagaa agcgatttta tggaagccat aaagaaaaat
     2281 gttgaatcct tgctaagaaa accatcaaca ttttctcgct taaatctcaa tactatttct
     2341 gaaaggaggt cttttttgcg atccttttta agaagacagg gtttcccata tcagtctgtg
     2401 gagacattta tcgaaacctt ggtactaaag gaaaacaaaa atgactagaa atactatttt
     2461 ccaatacttg acatacctta caaatagatt caataatatg tcagagaatt caagtaggag
     2521 gcgcatatcg aatggatgac gacatcctta ctattgaaga agtagcaaag tacttgcggg
     2581 tttccgaacg aacagtctac gactgggctc agaaaggcga aataccgtcc ggaaagatag
     2641 gcactgtctg gcgatttaaa aaaagtgaga tagaacgatg ggtaaacgag cgcctttctt
     2701 cgaatagacc tgtttcactc atcagcagtg taaaaatcca aaatatcatt tcccctgatc
     2761 ggattatttt tctaaattac ccctccaagc gcgatgcact aatcaaattg gcagaaaatc
     2821 tttcaacagc accccaaata aagaacaaag aagaattgat ctctgaaatt ctccgacgcg
     2881 aagaactcat gagtacagcg ataggaaaag gcattgctat cccccatgtg aggcttaatt
     2941 cggttacaga ccttgtggtt tcaataggaa tctctcgttg tgacattgtc gattttcaga
     3001 cgctcgatga tgtgccagtg cgattattgt tcatgattgc tgccgcatat aaccagcatg
     3061 catactattt gcaaactctg tcgtttttct ctgcaagact caaaatagtt gagcttcgag
     3121 atgggctttt agactgcaag accacgcaag aagcatatga tctgctcatt cagcaagatc
     3181 gatagaactt tattgcttct gtgagagcac ctgcagtgcg cccatgcgtt tctgtgggtg
     3241 cttttattta gctacgaagg aatcattctg tgtatttaaa aagccttgaa atatttggat
     3301 tcaaatcatt tgcggatcga atacgcatcg agttttcaaa aggaatctca gctctggtag
     3361 gtcctaatgg ttgcggcaaa tccaacatcg tagactctat caaatgggta gtaggtgaac
     3421 agtctgcgaa aagcctgcgc gcagaaacaa tggaagatgt catattcaac ggtactgaga
     3481 cgaggcgacc actttctgtt gcagaggtag caattacaat aaccaatgat gaaggagcgc
     3541 tacctctcga tgttcccgaa atcgaggtaa agcgaagaat ctatcgctct ggggaaaatg
     3601 aatatttcat aaacggcaag ccagcaaggt tgaaggagat tagggaactc ttttgggata
     3661 ctggtattgg caaatcagcc tattcagtga tggaacaggg gcgcatagat caggtgttat
     3721 cctcgaaacc tgaagatcgg cgttatctat tcgaagaggc agcgggtatc acgaagcaca
     3781 gagttcgtgc tcacgaagct gagcttaagc ttgccaaaac cgaagagcat ctccgccaaa
     3841 tagaagctat aatcagagaa gtcaaacaat cctacgaaaa cctaagagtc caatccgaca
     3901 agactatcaa atacaggcag ctaaaagata ggatatttga aacggagcta gatctatatt
     3961 tgatccgctt gcgccagcac atccgcgagc aggaccgagt taatgacctt tttaatcaaa
     4021 aaaagaaaga tcgcgaagca cttgtccagc gcatagattc tatgagccaa tctatgtccg
     4081 cggatctcga tatattgaac aagcttgaag caagactaat cgagatgcag aaactgatct
     4141 atggccttgc tgttgaaaag aatggcaaag aaaagcaaaa ggcgctgttg acagagcggc
     4201 ttcgcgagat caagtcaata atagagcaac tcgaagcaag agatcgtgct atcgcctcaa
     4261 aaatagaatc acttcaagaa gaagaaggcg ataaagaagc ggaatttgcc ggctataaaa
     4321 ctagaattag agaaatcgaa gccaatatac atactttcga tgagaatatc tctgcagctg
     4381 ctttattgct aaaagcaaat gaggaaagca taagacacaa tatcgaggaa tccaaggaaa
     4441 ttgctcaaag gacaacactt ctccatgaga aactggatac tatcacagaa agaatcgcag
     4501 agctcgtcga cgaacggctt cgcaaatcag aatcgcagat cgagcaaaag cagaagctcg
     4561 aagcacaaat acagcagaaa ataaaagaaa tctctatttc agcatcttct cgggcagata
     4621 gactccaaga ttataccaga attgcttctt ctttgagcgc ccaagatcgt tctcgtgaac
     4681 tcgagggctt ggtcgatgga ctacgagctt tatcttctct ttcctcggat cttgaaaggg
     4741 attttttaca atacatcgag atagagcctg gattcttaga cgagcttgtc gctcccgaag
     4801 gcgttatgac tcaaaaacgt cgcatcgatg cggaaatttt tgatcaaagc gagcgtatta
     4861 agagaataga agccgagaat cagggtctat cgctaagaaa tacagatcta gcaaagaaaa
     4921 tcgaagccta tcgcaagaca ctagaagagg ccaagctgga aaaagcgaag attcaagcac
     4981 agatggaagc tgcaaaagaa gcactttcgg tgctaaagag agaaatcgcc ggacaagaag
     5041 cttaccatcg cgaagttgct agcgagctct tagatcagca aagacgctgc gaaaagctcg
     5101 aagaagaaat caaattgtta caggaagagc ttgcagagat agagcataag ggtagaaatc
     5161 taacagagga aatgtcggag ctcgataaat ccattagtcg agcgactgaa gatcttggag
     5221 acaagcgcaa agagtcgaca gagctcgagc agaatctgca gtccattcaa caagagctcg
     5281 aaaacttaca tactgcaaaa gcgcaaagcg agacagagat tcgcaacctc aaggataatt
     5341 ttgcagatct ctattcgcga gatttactag ctttcgaaag ccgaatgtat gaaattcgct
     5401 ctcctgtttc tgaaatcaag gaaaagcttg tatccttaaa ggcttctcta tcgtcactag
     5461 gttctgtaaa ccttatggct cccgaggaat tcgagccagt gaaacagcgc tttgaatttc
     5521 tttcggggca atatgatgat ctcgtaaaag cgcgagcaga ccttgtaagg atcaccaatg
     5581 agataagaac cgaaagcgca cagctttttg tggaaaccta taataaaatc aaaaagaatt
     5641 ttcacaatat attccgaaga ctatttggtg gaggaagagc agagctacgg ctttcggatc
     5701 ccgaccatgt cctagagagt ggcatagaaa tctttgctca gcctccaggc aagaagctcg
     5761 aaaacatctc tttgctttcc ggaggagaaa ggacactcac ggctattgcg cttctttttg
     5821 caacctatat ggtcaaaccc tcgccattct gttttttgga cgagatagat gcggctctcg
     5881 atgaagccaa tatagtacag cttgtgaatc tcttgagaga attcggcaca atgagccaat
     5941 tcattgtgat cactcataat aagaagacag tggctagtgc cgacaccttg cttggtatca
     6001 cgatggagga atctggagtg agtaaggcta tcgcaatccg tgtccaaaat gagaatggtg
     6061 ttgtgcatcc agtctatgtt cccgatcagc ctttcgaaga agaggaagtc gattatgaag
     6121 aaggtagaca gctagaggcg aatcgatgaa tgccgaaatg ctaggtgaaa ggttgtttta
     6181 tctcgatcca tggcttaagg aagcggatgt cgttgttaag gctaccaaac ctgtagtcaa
     6241 tagaggctct actatattag gtgttttgct gagcgagaca atattctacc cagagggcgg
     6301 aggacagcca gcggacttag gttttattga agataagcag gttatggatg ttcaggagat
     6361 agaaggtgag atatggcatt atatcgagct gcaaaatgag aaagaggaag aaattttaaa
     6421 gcctggttca gccgtacatt tgagcctcga ttggccaagg cgtctctacc acatgcagca
     6481 gcatacagga caacatctac tttcagcggt gctcgaagag gaatatggca ttcatacgat
     6541 ttcttttcac cttggaacag agtattgcac aatcgatgtt tcagcaaaag atccagctga
     6601 gctgccgctt gcgcagatcg aaaccgaggt cgaggcctgg atagagaggg atgcagcggt
     6661 tttgatacat tactgtcctc cagaagatat ttcgaccttt agacttagaa aaaggcctcc
     6721 aataaacgaa gctgttattc gtgtcgtcga gattcaaggc tatgattgct ctccatgtgg
     6781 agggactcat ctggaacgaa caggccaagt tcgagctctc aaggtgcttt cgctcgagcg
     6841 ctataaaggc catgtacgca tgtattttgc atctggagct catgccatag agctcatgtc
     6901 cagggcttat gaggacaata gagaagctgc tactttgctt ggatccagca ttggtgatat
     6961 ttctatacga ttgcgtgaga ttctagaaaa gtctgcttca ttggaaaaaa gagccaagag
     7021 atgcttggta gagtatgctg aagccgaggc caagcttgct tcgatccatg caggaccctc
     7081 agacataatt cagtttacgc tagagaagga agatgcagat tggggtacta tgctttgcaa
     7141 agcagctgta tcgcttggaa ggccagcaat agcttcttgc cttcttgatt ccactattat
     7201 catacaagtc cctgcaaaag cgcgattccc agcgctttca gcggttcttt cagaaattat
     7261 gttgaattta ggtggaaaag gaggaggggg agaggctttt tatcgcgcaa gctttgcttc
     7321 agcggatatg gcgaaacaat tcgccttaaa agcaaaagaa gtattagaag gatttcaagc
     7381 tacataataa ggctttgatt ctatgctata tccctaagac aacaatctat aagatcccta
     7441 acgccttaaa gcgccaaagg ccttcgattg tcagctagtc tctgactgag ataatcctta
     7501 tcgagattca gcttttggat aaaaaagcgc atctgctctt cggataacag gttttgctcg
     7561 gcatacagat atacaagggc tcgttcggct attaaaggta ccttgagaga attcatctgc
     7621 atctcacgtt ctatctcttc tgcacaacga cctcgtatag atgaatagag cctcgattct
     7681 tcgatatttc cctggagagc agccaactca gcgaagagtg gcgcaatatt tccacgggca
     7741 gcaagctcgc caaaaggctt aagagtgaag aaagtatatt cctttgcggg cagataatga
     7801 tgatcgtcgc atctggtgca gtagtttggt tcacgtccat cgtgcttggt acggtcccct
     7861 cctattatga ttagaggatc gaagtatagg gcggggcact cctgaccatc cactagataa
     7921 tatcgatatg tatagagaga atcccttaga cattcttcgt gttcgcagta agctgtaagt
     7981 ggtactacat ccgttgcggt ctccaggaga agtcgagcgg tctgattgaa gatttcgcga
     8041 cgaaaattta ataacagtgt ggggaaaatg aaacaaagtc ctctcaattc gccttcatct
     8101 tttgtgagat atgctacacg ctcatcgaaa aatgccgctt cgtcgacgat ccaggtacct
     8161 acttcaggat tttcagctat gagcacttcg agcccaaaag agtcttttgc agcacctatt
     8221 ctatttccgc atctttcgta tcctccccta tatgctaatg catcatcagg ataatcgggg
     8281 aaacgactct tatctaaagc agagcgaact acaaagacat tccttcgatc gacattggcc
     8341 gtgcttgtca atcttcttac agcacctttt tttttaatcg ctacacggct atctcgccaa
     8401 acacgcgcag caaattcagt tttgcctgaa cccatcgggc caattacaag gatgcgcttg
     8461 tttgccgtaa taaaatcgtg atgcttagcc attgcatgga tatttaacag cggaaagcca
     8521 aggcttttca ggaattcgcc tgtctcatcg atgttgttca tagttcttcc tggtctttca
     8581 gtttatctca atgaaatgta tgagcttgat acctttgcca cattggggct tatgctagga
     8641 ttgccaaagt gaaggtattt ataagggtgt atgtcaaggg cattctgata ccatccttgg
     8701 atgaaatcga tatcaatcac agatgctgcg aagttatgat atattggtag acaagctgcg
     8761 ccctggagca gctcggtttc tgctttagcc agaatactca tgcgcttttc atcgctagca
     8821 tcgtaagact gtatcatgaa gtccatgaaa accgaatcat tataacctgc gatattgagg
     8881 ggcgcgtcgc tagtccacat ctgaaggaaa gcttccgggt ctgcgaagtc gccgatccag
     8941 gtttcatgag ctatagtaaa ggatgaagcc tccgacctgt tgcctatgct ctcataatag
     9001 gatgaaggtt gcattggctt tagagttact tttacatcga gcgctttttc ccactcagtc
     9061 ttaaaagtat tggcgataga acgcggtctt tcataatctg caaatgctat gattatttcg
     9121 ggaaggcctt ggcccttggg gtatccggac tcttcgagaa gcttcatagc ttccgtgcta
     9181 tcagtttttt ctattccttt ggtcttggag taaccaggca aggggagtac cagagtcttc
     9241 gcaggaagca tatagaattc gcttgagcgg atactatccc aaggtagaag aagagcaaga
     9301 gctcgcctta ctctcttgtc attccagggc tctgaacgac agttgaaaaa ccaataatga
     9361 gtcgaataca taggagaaat ctgaatcgct gtttgaagga gcaccttgtc ataattcccg
     9421 ggtccatcga gccagtggac atatccagta ttgaaaaggg aagtagctag ttcatcgtcg
     9481 tctgtaaaca tcattctgag ttttggaata ctcacatttt tggagtccca gtaagcttcg
     9541 ttcttttcaa gaataagctc tgtatctgtc atacttactg gcttataggg gccgctgaca
     9601 gggtagggaa ttgcgtttct ccaatctcct gcatctatca tcgatggatg aattacagag
     9661 aaagattgat ggcaaaggag ccttgtaaag taagcgacag gtcgcacaag cgttatttcg
     9721 agcgtattag gggaagctgc cttgattcca acatgctctg gattcttatc caagcccatg
     9781 cgatattgtt ttgctccctc gattatatcg aagaaagttg catattcagc gtttatgtct
     9841 agcattctaa accatgaacg cacaaagtct tcagcagtga tgggtgagcc atcggaccag
     9901 agagcattgt ctcgaatggt aaagcgatat agtttcttat ccactgagcg ttcccagttc
     9961 tgggctaggg cacgcattgg ttcgagagtg ttgggatcat agttgaaaag gccctcatat
    10021 atagcagtgt ggatctgagc ttcattcgag aagatagcct tctgaggatc tagttctggc
    10081 agttgagagc catatactgt cacgaattcc gtttcttgca tttgagcagc aagaggcaaa
    10141 aaagctaggg ctaaaaaaga tacaaccaga aaaagccttg agttcataga actaattatg
    10201 caatataata gtttggaagg caataaacat gggaattgaa aaagaattta tcccggagcg
    10261 gctaattgtt ggagtgcttt cttcggatac ggaagcggaa atagctgctc tcgatgcaat
    10321 gataggactg tatggaccgc tttgcttcca atcaagcaaa gaatcttttt tgtggacaaa
    10381 atactactgt ccggaaatgg gagaaaatat cttacgatct tactgggctt ttagagagct
    10441 aatagatcct tccaaactat cgatgatcaa gcgcgctacc gacgctatag agctaaggct
    10501 tgcaaaaaat agctgcaggt tggtcaatct cgatccaggc atactcggga ccgctcgatt
    10561 ctgtctagcc acaaccaaag accgttccca ccgtatcccc ctctcggatg gaatctatgc
    10621 agaattgacc ttgatctttg agcacggaga attcaaagcg cttccatgga cctatcctga
    10681 ttgggcttcg gagccagtta ggaaaatgat gtcagagctt cgagctggac ttcttggcga
    10741 tctaaggcat ctgcatctta tttgaatatg ccccaagaag agacaattct ttagattatt
    10801 ctcttatttc agcatagaat tgatatcatg caccctcagc acaccatgtc ttttcatcca
    10861 gtcggtaatc ccactaagta tttcttgcgg agcatgaggg tttgagaaca gtgcggttcc
    10921 aatctgcact gcctgagcac cggcaaggaa atattgaatc gcatcacttg cacgagcgat
    10981 gccgccgact cctatcacag gaatagatac tgagcggctg acacgatagg ttgcagctac
    11041 tccaatagga cggatagcag gacccgagag ccctcctatg cccttggcaa tagcaggttt
    11101 catgcgatct gtatctatga ccatgcctaa aactgtgttg atacaggata ctgcatcggc
    11161 cccgccttct tctgcggcgc gagctatttc tgctatatct gtaacattag ggctaagttt
    11221 cacaattaga ggcttatcgg tgcgctttct taaggagcga gtcaatcgtt ttacaatgac
    11281 agggtcgatt ccaaaagaca ttcctccctc gcacacattg ggacaagaga cattgatttc
    11341 atagccatcg ataccaattt ttggtaaagg ttcagataat gaaagccgct cctcgatacc
    11401 atcaataagc atggcatatt cgtcttcggt cgagccagcg atattagcta taatagcaca
    11461 gctataattt cttaagagtg gcagtttttc tttgagaaaa cactcgaggc cgggattagc
    11521 tagacctatg gaatttataa gtcccattgg tgtttcaaca agtcgcggtg ctggattgcc
    11581 ttctcttggt tcaagggtta ccgtttttgt atatagagcg cctaattcat cgatattaac
    11641 aagctcctcg tattcttcgc catatccaaa gataccagat gcaactccta tgggatttgc
    11701 catcttaaga gaaccaattt ttacagataa gtctggctcg aaagaagcag aagaattatt
    11761 ttggccttct attgagttct tca
//
LOCUS       DAOSOB010000059        11506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_5598_length_11506_cov_67.672518, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000059 DAOSOB010000000
VERSION     DAOSOB010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11506
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5598_length_11506_cov_67.672518"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="PLS88_06875"
     CDS             <1..1298
                     /locus_tag="PLS88_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07102.1"
                     /translation="LQKTINGIQTDFGDAFRKAAPIPTLCRPDPKVDPAFELFLKNKS
                     VVNWYPRIQAMKSKGLLGEQMQYQPNEAHLTAQHVAFLDLDALYFELERFKAERGWFN
                     LNIPRSVIGELLVDPSWYTLLIPKAELAFGSYDRVRTWQEIALALLKKYLERFYTFQK
                     QKWELPHLEVRYLEDFDPNLLGVGEKPSDPYYRVAVDKSDQELIASLRQLKERIENRS
                     ETEWNFRNLDAIWFAGHLFQPLLIIDTDRQGYSAEEGTGGHLKLEISPVPLNRGEGQF
                     VRDIKEFYAREKAKGEAGFFAHRELFMLRNLSRGKGVGFFEAGNFHPDFMLWLVDADG
                     RQHVRFVDPKGTEHIEFTDAKIDFYQTIKDIEKRLADPDLTLDSYILSDTSSMDMMLH
                     WNVDKAKMLAHHVVFLYEDKEHYLQTILADNKEDFRPQA"
     gene            complement(1377..2684)
                     /locus_tag="PLS88_06880"
     CDS             complement(1377..2684)
                     /locus_tag="PLS88_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU07103.1"
                     /translation="MKKMKKLMVLFLFAVAALQAFGLEGLIETKSNTPLSSSALWTTQ
                     DAKKDKVVVVSDIHLGVLDKYSENLKNRAVFIEFLKRLQNTEDVGELVIAGDFLDEWY
                     LPLTYPAYSDSSKFYWQVVKNNQAVFDELKALMEKGIKLTYVPGNHDMTLEGDILDKA
                     LPRINQARDVNGLGKYVTGVRDEIIIEHGHRYDVFSAPDSVSNKDITGDAPSILPPGY
                     FYARIAASWVLQGKPMIKKDYPEIDKVPDPKTDPDQYGAYLYYRIWSGEINRITPFER
                     FEDRVIDIKINGYNGKYSIKDFYPVVQADGTISAPTLFKNYQRDWDKIQENNLVSIKT
                     SFAESVAGALDSEYFFKQAKKQYLENTNWKVDVVVFGHTHNPMLRKLPNAEGKYYVNS
                     GTWIDENPDHPAASTFVVVTSREDTDIVELYEYYRDGTIRKLD"
     gene            complement(3305..4858)
                     /locus_tag="PLS88_06885"
     CDS             complement(3305..4858)
                     /locus_tag="PLS88_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOU07104.1"
                     /translation="MSLDTDNSVLYPNNAPLGLLSLGARTQLLQPDRVLEIVFQSLKE
                     LVDYELAVVLSIEQGNVLRVRKAFGPLAIPALENFSISLDQRKDIAEILQSKRPKLFH
                     QSEAHIDTYYGIIDLPSGHSCLVSPLYIEDLLVGMLTIDHRSCNRFSPSIVQFIGTIS
                     RLISVSLIQTDASTDLLKRTRVILDERNRLLDENAQVFHDLIGVSPTWMRVLDDIRLV
                     AASELPIFITGETGTGKEQVARTIHAMSTRNQGPFIAVNCSGLVTTLAESELFGHEKG
                     AFTGATTSRRGRFEIADGGTLFLDELGDLPLEIQPKLLRVLQDGIIERVGAEHPLHVD
                     VRIIAATNKNLEDEMTKGNFREDLWYRINVFPIHLPPLRERDHDAILIAQYFLEKIRK
                     RPGYNSVEFSDQAIDKILQIPWYGNVRELRNSIERAAILARGGIIHAEHIISYRDQRQ
                     AMHENVLIEKSEIGSLEEIEKHHIEKILMQTNGKIYGTDGAAAILGLKPSTLQSKMKK
                     LGIQRPARS"
     gene            5108..5755
                     /locus_tag="PLS88_06890"
     CDS             5108..5755
                     /locus_tag="PLS88_06890"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOU07105.1"
                     /translation="MEQQAVHMPLLGDPFPQVSLQTTQGMLNLPGDLKGSWFILFSHP
                     ADFTPVCTSEFVAFQKLIPEFEKLGVKLIGMSIDQITSHIKWLEWIKEKLGVEITFPL
                     AAANDAIAMTLGMLHSGKGTNTVRAVYIVDPNGIIRLILYYPQEIGRFMPEVLRAVKA
                     LQISDKNHVSMPANWPNNELIGDHVIIPPPKTVAEAADRLQHYEGFDWWFCHKSL"
     gene            6008..6475
                     /gene="bfr"
                     /locus_tag="PLS88_06895"
     CDS             6008..6475
                     /gene="bfr"
                     /locus_tag="PLS88_06895"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOU07106.1"
                     /translation="MKGDPKVIDVLNSLLADELTAVNQYMVHSEMCDDWGYEKLHKSI
                     EKRAIDEMKHAEKLIGRILFLEGTPIVSKYNEIHIGADVSEMYKNDHLAEADAIKSYN
                     SAIALCGEARDYATRDILEAILKDEDAHIDNIEAIQDEIKQMGIQVFLSMQLG"
     gene            complement(6846..7661)
                     /locus_tag="PLS88_06900"
     CDS             complement(6846..7661)
                     /locus_tag="PLS88_06900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU07107.1"
                     /translation="MEEYTKYYENKVAVVTGAASGIGLALCELLLEYGARTIVMADLN
                     GEKLKAESQRLELAYPKKVLAIQTDVSQKESVEAMIAEAARFGGGQIHFLFNNAGLGL
                     WKRFDDTTDKDWNFAFDVNFFSAFHGIRAVLPIMRAQGGGHIANTASGAVYSPMAEQS
                     MYCATKSALLGLTGALRYELWDENIRFSTVIPGSVATSIWDNVGGPPASAISPRESAK
                     GILKGLAANERVVIVTEADRDAARNTYRPEIAQHTDEYFLGVARQRREGKLGL"
     gene            complement(7755..8540)
                     /locus_tag="PLS88_06905"
     CDS             complement(7755..8540)
                     /locus_tag="PLS88_06905"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOU07108.1"
                     /translation="MFKFEEGKCYKMPAHFGGVPFDPDAKAIYDDVVTIDFCYTTDAE
                     KLSNYLPEGLELIKPELTVEYQQCRQVEWMAGSAYNLVCVTVPVRFNGRHDHLEGIFV
                     LVMWENKTTPILTGNMMGVPKIYADIEDLHILADNFRTCMSFEGKTFLELEAIRQTAM
                     SGDQVKAMDTDLNTIGWRYIPKVGAPGADLSQPILFPMHRTPKVGWTGTGTLKWTALT
                     WAQNPLQYYIIDQVAELPILDIAPIVITQGEQVLTEARGRVLS"
     gene            complement(8567..9754)
                     /locus_tag="PLS88_06910"
     CDS             complement(8567..9754)
                     /locus_tag="PLS88_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07109.1"
                     /translation="MKLREAKLPLEVIILIIAGILVLTTGVLLFSVYKGALPYFENGL
                     YGLLLIIFALQTITLGRTPFGDLPRSTLVLFLGLLIAVIGMVTCLIPISIRLPRILLF
                     LCFGPGGLLLFLQLLFAKDKLRRWAHYGGIFRHLIVACGAVYVLSMVIALLLLNQRLL
                     TTPLTAVVILVFGVAVFYLAIVLEKIDRTYPEAAKSAEGNVKLSTEQVMILFTGIFMV
                     IIGIVLIPVNLGILPFSASAQLGILMVIFAIQMLAFGNTPIGPFPRSWIVLAFGLLFA
                     LLGAISCIIPEILVQFLTILVGVLNILGGVLALAKMLPSKQRRPEEPRKPTPPILTKL
                     FATQLTLNLLQILFGMSMLVPGMVPGLVIGVILAANGCVLLYLLHILLMLDTMRSEME
                     AAK"
     gene            complement(9929..10450)
                     /locus_tag="PLS88_06915"
     CDS             complement(9929..10450)
                     /locus_tag="PLS88_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07110.1"
                     /translation="MKKLLIVVFSMVSSLCLFPQPMENSVNYVSILLKNEAYLGIKSI
                     DWRYENNQYVFTLALNNALLFEKLTNKNTFIEWDIFIDIDSNTKTYSWLPAPEYTNDL
                     DVDYLVRIIMTDTGYSSRITNSKKKVNKSFQYRIEENTITYWIGENDLESKDQFFQQH
                     QLEPMLARAIRNH"
     gene            10582..10815
                     /locus_tag="PLS88_06920"
     CDS             10582..10815
                     /locus_tag="PLS88_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07111.1"
                     /translation="MRVSKKFGIAVGCVLLILLLSSCVDVEYRISINSDHSADVFYQI
                     LMNPLFASLMQGDADQGPLDSMREYCYEPCVFF"
     gene            complement(10836..>11506)
                     /locus_tag="PLS88_06925"
     CDS             complement(10836..>11506)
                     /locus_tag="PLS88_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07112.1"
                     /translation="AHSQYILSLTHATLRRSGRCTDSQKRRKIILYRSLVFEKAALER
                     SFSELLDQLYRERCSAYSSPLILITDEKCEYARALKLHPSRFSIHHLTVNSKVPRTFQ
                     NPLFASNYLDRELRKDLADHRRETVCFPRNVANMLNRLVVYLGWHNYEKPYRIGRHIV
                     MTHAEVAGIERKAICKAREKQFQERAFLSRAGLSLLDKKLWLRSFPTPLKRKAEYVPA
                     YAYA"
BASE COUNT         3126 a         2579 c         2635 g         3166 t
ORIGIN      
        1 ggctccaaaa gaccatcaat ggcattcaaa ctgactttgg tgatgctttc cgaaaagctg
       61 cgcctatacc gacgttgtgc aggcccgatc ccaaggtcga tcctgcgttc gagttgtttc
      121 ttaagaacaa aagcgtggtg aattggtatc cgagaattca ggccatgaag tcaaagggct
      181 tgctaggcga gcaaatgcaa tatcagccca atgaagcaca cctgacggca cagcatgttg
      241 cattcctcga tttggacgcg ctctatttcg agcttgagcg tttcaaagcc gaacgcggct
      301 ggttcaatct caacattcca cgctccgtga tcggcgaact ccttgtcgat ccgtcatggt
      361 ataccttgct cattccaaag gccgaacttg catttggttc gtatgaccgc gtgcgtacct
      421 ggcaagagat cgcgctcgcg ctcctcaaga agtatctcga gcgcttctat accttccaga
      481 agcaaaaatg ggagttaccg catcttgaag tgcgctactt ggaggatttc gatcccaatt
      541 tgctgggcgt tggggaaaaa cccagcgatc cttattatcg cgtcgctgta gacaagtcgg
      601 accaagaact cattgcaagt ctacgacagc tgaaggagag aattgaaaat cgttctgaaa
      661 ccgaatggaa tttccgaaat ttggatgcaa tctggttcgc gggtcacctt tttcagccgc
      721 tcttgataat cgataccgat agacagggat attctgctga agaaggcaca ggtggacacc
      781 tcaaattgga gatcagccct gtgcccctca acaggggcga aggccagttt gtacgcgata
      841 ttaaggagtt ttatgcccga gagaaagcca agggcgaggc cggttttttt gcgcaccgag
      901 agctttttat gctgcgcaac ttgagtaggg gcaaaggcgt gggctttttt gaagcaggca
      961 acttccatcc cgacttcatg ctatggcttg tcgatgcgga tggccgccag cacgtgcgct
     1021 ttgtcgatcc gaaaggaaca gagcacatcg agtttacgga tgccaaaatt gatttctacc
     1081 aaaccatcaa ggacatcgag aaacgccttg ccgaccctga cctaaccctc gactcgtaca
     1141 ttctttcgga tacatcctcc atggacatga tgctgcactg gaacgtcgac aaagccaaga
     1201 tgcttgcaca tcatgtagtt ttcctgtacg aggataaaga gcactaccta cagacgattc
     1261 tggcagataa taaggaagat ttccgtccac aagcatagcg tagcaaaaac ttatctctcg
     1321 ccatcgatca aggtcaaaat ctaaggatgt gccactacaa ggatgcacaa aaaggttcag
     1381 tccaatttcc taattgtccc atcgcggtaa tactcgtaca gctccacaat atcagtatct
     1441 tctctgctcg taacaacgac aaaggtgctc gctgcaggat gatcgggatt ctcgtcgatc
     1501 caggtgccgc tgtttacgta atattttcct tcagcattag gaagttttct aagcattggg
     1561 ttgtgagtgt gcccgaatac cactacgtcg accttccaat tcgtgttctc gaggtactgt
     1621 ttcttcgctt gcttgaagaa gtactcgctg tctagcgcgc cagctacact ctccgcgaag
     1681 gaggtcttaa tcgagaccaa gttgttttcc tgaatcttgt cccagtctcg ctgatagttt
     1741 ttgaagagag ttggcgcgct aatagttcca tcggcttgga caacagggta aaaatccttg
     1801 atggagtatt ttccattata gccattgatt tttatatcga tcacgcgatc ctcgaaacgt
     1861 tcaaagggtg taattcggtt tatctcgccg ctccagatcc tgtagtatag ataagcgccg
     1921 tactggtctg gatcggtttt tggatctggc actttgtcaa tctccggata atctttcttg
     1981 atcattggct tgccttgcag aacccagctg gcggcaatcc tggcgtagaa atagcccggt
     2041 gggaggatag atggcgcatc ccctgttata tctttgtttg agacgctgtc gggcgcggaa
     2101 aagacatcat atctatgtcc atgctcgatt atgatctcgt ctcggacccc ggtcacgtat
     2161 tttcctaatc cattgacatc tcttgcttgg ttgatcctgg ggagagcctt gtctagtata
     2221 tcgccttcca atgtcatgtc atgattaccg ggcacatagg tcagctttat gcctttctcc
     2281 atcagggctt tgagttcatc aaaaaccgcc tgattgttct ttacgacttg ccaatagaat
     2341 ttgctcgaat cgctatacgc ggggtaggtt agcggaaggt accattcatc gaggaaatcg
     2401 ccagctataa ccaattctcc tacatcctct gtattctgga ggcgtttcag gaactcgatg
     2461 aataccgctc ggtttttaag gttttcagag tatttgtcta gtactcccag atgaatatcg
     2521 ctaactacga ctactttatc ttttttcgcg tcttgggttg tccacaatgc cgatgaactt
     2581 aaaggggtgt tgcttttcgt ctctattagc ccttccaaac caaaggcttg cagcgctgca
     2641 actgcaaaaa ggaatagaac catcaatttt ttcatctttt tcatcattcc gcctccaata
     2701 gaaaaacata ttgcacgtgc acacggcaaa gatggtgtaa tgtaaggcgg attggttcgt
     2761 caaggccact tttagtaaga atcaaattgg agcgtcgacc catgacagtc ataggagcaa
     2821 cgctcccaag gtcactttta gtaagaatca aattggagcg tcgacaaaga tgaaatgctt
     2881 gcacatcaca ttgcatttct ctatgaggat agggagagct atctaaagac tattttgatg
     2941 ggcaatgtgg aaaatcatag tcagcaagaa atagaggaac aaatttgata aaagtccaat
     3001 atcaaaaagt aagattcccc tcgcgctgtt tgatagattc gaaggaaaga atgtctattt
     3061 tatcgattcg acagacttga caggcaaagt tagcgaaagg tcttatccga tagaatcata
     3121 taaaatcatt gaatatggaa gactgcatgt ctatcgttag agaatttgcc tagaaaattc
     3181 gccaaaaaga acaaaaagaa gattaagaat atcgaaagac tgccttcatg gtccgttttt
     3241 aggacgccat aatcaaagcc tggcttgagt tataatctat gccaattaat aagcagctct
     3301 tagctcacga tcttgcaggt cgctggattc ctaatttctt cattttgctt tgaagggtgc
     3361 tgggtttgag accgagtatt gcggcagctc catcggtacc atagattttt ccattggttt
     3421 gcatgaggat cttctcgata tgatgtttct cgatttcctc tagtgatcct atttcgcttt
     3481 tttctatgag cacattctca tgcatcgctt gtctttggtc tctataggaa attatatgct
     3541 ctgcgtgaat aatgcctcct cttgcaagaa tagcggcacg ctctatgcta tttcttagct
     3601 cgcgaacatt gccataccag gggatttgca aaatcttgtc tatcgcttga tcggagaatt
     3661 ccactgagtt atatcctggc ctttttctta ttttttctag gaaatattga gcaatgagta
     3721 ttgcatcatg atcgcgttcg cgaaggggtg gaaggtgaat tgggaaaaca tttatcctat
     3781 accatagatc ttctcggaag ttgccttttg tcatttcatc ttcgagattt ttgttcgttg
     3841 cggcgatgat acggacatcg acatggagag gatgctcagc tccgacgcgc tctattatgc
     3901 catcctgtaa tacgcgcaaa agtttaggct gaatctcgag cggcaaatct cccaattcgt
     3961 cgagaaaaag agtaccacca tcggcaattt cgaatcggcc tcgtctggag gtagtagcgc
     4021 ctgtaaaagc ccccttttca tgaccaaaaa gctcgctctc ggcaagtgtg gtcacgaggc
     4081 cggaacaatt gacagctata aaggggcctt gatttcgggt cgacatcgca tgaatggttc
     4141 ttgctacttg ttctttgcca gtgccagtct cccccgtaat gaagatgggg agctctgaag
     4201 ccgcgacaag tcgtatatca tccaggacgc gcatccatgt tggagaaacg cctatgaggt
     4261 catgaaatac ctgtgcattc tcatcgagta gtctatttcg ttcatcgaga atcacacgag
     4321 tgcgtttcaa aagatctgta gaagcgtctg tctgaataag cgacaccgaa ataagtctcg
     4381 agattgttcc aataaactgc acaatagatg gtgagaatcg attacaggaa cgatggtcaa
     4441 tggtaagcat gcccacaaga aggtcttcaa tatagagtgg cgaaacaagg catgaatgcc
     4501 cggatggcaa atcgataatt ccgtaataag tatctatatg tgcttccgac tgatggaaaa
     4561 gcttaggtcg cttagattgg agaatctctg cgatatcttt acgctgatcg agactaatag
     4621 aaaaattttc tagtgctggt attgctagcg gaccgaaagc ttttcggact ctaagcacat
     4681 ttccttgttc gatggataaa accacagcaa gctcgtagtc gactagttct ttcaaggatt
     4741 gaaatactat ctcgagtact ctatccggtt gcagcaattg ggttctcgcg cctaaagaca
     4801 acaatcccaa aggagcatta ttgggataga gtaccgaatt atcagtatcc aacgacatac
     4861 agtcatttaa taccgatatt tcggtgctgt caagataact ctctcctttt atcctatgct
     4921 cttacccatc agtgaatgat aatcatctga aaaaggattg agcgataaag acttagaaat
     4981 ttaaagaccc ttattatttt ttgcagataa ttggcactat tcttgcttaa aattcttgtg
     5041 cctatttctc ccaaagagaa taggatgcat cacaatgcag aaatcttgtt ctaaaaggag
     5101 agttgctatg gaacaacaag cagtacatat gccgctttta ggggatccat tccctcaggt
     5161 cagccttcag actacgcaag gaatgctaaa tctacctgga gatcttaaag gttcgtggtt
     5221 cattcttttc agccatcccg ccgactttac ccctgtgtgc acaagcgaat ttgtagcttt
     5281 tcagaaactg attccagaat tcgaaaagct cggcgtcaag ctgatcggaa tgtcgataga
     5341 tcaaataaca agccatatca aatggctaga gtggataaag gaaaagctcg gtgtcgaaat
     5401 aaccttccct cttgctgcag caaacgatgc tattgcaatg acccttggca tgcttcactc
     5461 cgggaaaggc accaatactg tgcgagcagt ctacattgta gatcctaatg gcattattcg
     5521 tcttatactc tattatccac aagaaatagg acgatttatg ccagaggttc ttcgagcagt
     5581 aaaggcatta cagatctcgg ataagaatca tgtcagcatg ccggcaaatt ggccaaataa
     5641 tgagctaatt ggcgaccacg tgattattcc gccgccaaag acagttgccg aggcggcaga
     5701 tcgccttcag cattatgaag gcttcgattg gtggttctgc cacaaatcgc tgtagaaaca
     5761 tccttgaagg gttaaccatc ccgcatgtat gaggaacatc cagtatcaat cttgctattg
     5821 ttaggcagag cgctcgagta tgcgctctgc cttaaggatc tgagataatt ttatgggtaa
     5881 accaatctct cttcattatt tctgaggata catatcatgc ttttcactat ttactgcctc
     5941 tgattggctg tatatgatac ttttaaagga caagctcaaa taatagaaat ttggaggagg
     6001 aacaacaatg aaaggagatc ccaaagtaat cgatgtgctc aattcccttc tggcggatga
     6061 attgaccgca gttaaccaat acatggtaca ttccgagatg tgcgatgact ggggatatga
     6121 gaagcttcat aagtccatcg agaaacgagc tatcgatgag atgaaacatg cagagaagct
     6181 gattggtcgt attctttttt tggaaggcac gccaattgtc agtaagtaca acgaaatcca
     6241 tattggtgca gatgtatccg agatgtataa aaacgatcat ttggcggaag cagatgcgat
     6301 aaagagctat aattctgcca ttgccctttg tggagaagct cgggattatg ctacgcgcga
     6361 tattctagaa gcgattctca aggacgaaga tgcccatatc gataatatcg aagccataca
     6421 ggatgaaatc aagcagatgg gcatacaggt tttcctttcc atgcagctag gctagctctt
     6481 gctatattat tgaataaaaa ggctatatcg cagctgttta tggtcgcatg acaaatttgt
     6541 gcagggttag ctaagcagca ttgcaaagta ggagcgcaca gtggcgcacg gcaaatttgt
     6601 gcaaagctag ctaagcagcg caaacttgac aatgctatta gacagcctaa aaatatatca
     6661 ttctgccaaa agtcgtcctg acaggtatca ttgttagttt tatctcgctt gcatttctgc
     6721 tatcgcttgt gtttctctac tggacaccta cattataaat catggagctg gaatgggaat
     6781 gtagcctctc tattcgttat gtcttcgcgg tctttaggtc cacgggagaa agcgccatag
     6841 cttattcaga ggcctagctt tccttctcgt ctttgtcgag cgactcctag gaaatattca
     6901 tccgtatgtt gggcaatctc ggggcggtac gtattcctgg cggcgtcgcg atcggcttcc
     6961 gtgacgataa cgaccctttc attggctgcc agtcctttta gaatgccttt tgccgattct
     7021 ctaggcgaaa tggcggaagc gggagggcct ccgacattat cccaaattga ggtggcaacg
     7081 gagcctggaa taacggttga aaagcggata ttctcgtccc agagttcata acgcagcgcg
     7141 ccggtaagcc caagcaaagc ggattttgtg gcacagtaca tactttgctc cgccattggc
     7201 gagtaaacgg caccggaagc cgtgtttgca atatggccgc ctccctgcgc ccgcatgatg
     7261 ggcagaaccg cccgaatgcc atggaacgcg ctgaagaaat ttacatcgaa ggcaaaattc
     7321 caatccttgt cagttgtgtc gtcaaaacgc ttccacaagc caaggcctgc gttgttgaaa
     7381 agaaaatgaa tctggcctcc tccgaatcta gctgcttccg cgatcatcgc ttccacactt
     7441 tctttttggg agacatccgt ctggattgca agcacttttt tggggtatgc aagctctagt
     7501 cgttggcttt ccgccttcaa tttctcgccg ttaaggtccg ccatgacgat ggttcttgca
     7561 ccatactcca agagcaactc acaaagcgcg aggcctatgc ctgaagcggc accggtgaca
     7621 acggcaactt tgttttcgta gtatttcgtg tattcttcca taatttatga cctcctttgg
     7681 ataccactat tcaccgatgg ataccatttc ttattagact atttgttgct gcggacatac
     7741 tgccgagagc gactttaact taagacacgc cctcgcgctt ccgtcaaaac ttgctcgcct
     7801 tgcgttatca cgataggcgc tatgtcgagt atgggcagct ctgccacttg atcgattatg
     7861 tagtactgca gcgggttttg tgcccaggtc aaagcggtcc atttcaacgt tcctgtccca
     7921 gtccagccga cctttggggt gcggtgcatt ggaaagagaa tcggttgact caaatccgct
     7981 cccggagcgc ccacttttgg tatatagcgc cacccaatgg tattgaggtc ggtgtccatt
     8041 gcctttactt gatccccgct catggctgtc tgcctaatcg cttccaactc taggaacgtt
     8101 ttgccctcaa aactcatgca agttcggaag ttgtcggcaa gaatatgtaa atcttcaatg
     8161 tcggcataaa tcttggggac gcccatcatg ttccctgtca gaatcggcgt tgtcttgttt
     8221 tcccacatga cgagcacaaa tatcccttcc agatggtcgt gacggccatt gaaccgtacc
     8281 ggaacggtaa cacagactag gttgtaggcc gagccggcca tccattcgac ctgtctgcac
     8341 tgctgatatt caacggtcaa ttcgggcttt atgagctcga gaccttccgg caggtagttg
     8401 gaaagcttct cggcgtccgt ggtgtagcaa aagtcgatcg taacaacgtc gtcgtaaatg
     8461 gctttggcat cgggatcgaa aggaaccccg ccaaagtgtg ctggcatttt gtagcatttt
     8521 ccttcttcaa acttgaacat cctaaacctc cttgcaaaga tacatcttat ttggcagctt
     8581 ccatctcgct gcgcattgta tccagcatga gtaagatgtg gagaagatag aggagcacac
     8641 acccatttgc agcaaggatt acaccgataa ccagccctgg aaccatgcca ggaacgagca
     8701 tcgacatgcc aaaaaggatt tgtaggaggt tgagggtaag ctgtgttgca aagagcttag
     8761 tgagtattgg gggagtgggt ttgcgcggtt cctctgggcg tcgctgtttg gaaggcagca
     8821 tttttgccag agctagtacg ccgcccaaaa tattgaggac gccgacaaga attgtcagaa
     8881 actgtaccaa aatttctgga atgatgcatg atatagctcc caatagggca aaaagaagac
     8941 caaacgcaag cactatccag ctgcgcggaa atgggccaat tggagtgttt ccgaaggcaa
     9001 gcatctggat agcgaaaatt accataagaa ttcctaactg tgcgcttgct gaaaagggaa
     9061 gtatgcccaa attgacagga atcaacacaa tgccgatgat caccatgaaa ataccagtga
     9121 acaatatcat cacctgctcg gtggagagct tgacattgcc ttctgcgctt tttgctgctt
     9181 ctggatatgt tcgatctatc ttttcgagta cgatcgcaag ataaaagact gccacgccaa
     9241 aaacgaggat caccaccgcc gtgagcggcg tcgtcaaaag gcgctggttc aaaaggagca
     9301 gcgcaatgac cattgacaga acatagaccg cgccacacgc aacaatgaga tgtcgaaaaa
     9361 tgcccccata gtgtgcccaa cgcctgagct tgtctttggc aaacagaagc tgtaaaaaaa
     9421 gcagcaagcc gcctgggcca aaacagagaa acaacagaat gcgaggaagg cgaatcgata
     9481 tcggaatgag acaggtcacc atgccaatta ctgcgatcaa caagccaaga aagagcacga
     9541 gcgttgacct tggtagatct ccaaacggag ttctgccgag tgttattgtt tggagcgcaa
     9601 atatgataag aagcaaacca tagagcccgt tttcgaagta agggagcgct cccttataca
     9661 cggagaaaag caaaacccca gtcgttagta ccaaaatccc agcgataatg aggattatca
     9721 cctcgagagg aagctttgct tccctcaatt tcatatattc cgtccttcgc tgataacagc
     9781 gctgatagca gcaacgatat tgccgaggat ctcgatatca gaaacaataa gaaagaatat
     9841 cgagtcaatg cgactgttgg actccaatac tttttctatt gtctacttac tacatagtgc
     9901 ctggtttctg gggaattgtc aaagtacatc aatgattgcg aattgccctt gccaacatag
     9961 gctccaactg atgctgctga aaaaattgat ctttactttc aagatcattt tccccgatcc
    10021 aataagtaat agtattctcc tcaattctat actgaaatga tttgttgact tttttcttcg
    10081 agtttgttat tcgagaacta tatccagtat cagtcattat tattctgacc aaataatcaa
    10141 catctaaatc attcgtatat tcaggtgccg gcaaccacga atatgttttg gtattcgaat
    10201 caatatcgat aaaaatgtcc cattctatga atgtattttt attggtgagt ttttcgaaaa
    10261 gaagagcgtt gttgagggcc aaggtgaaca catattgatt attttcatat ctccaatcaa
    10321 tgctttttat gccaaggtat gcttcatttt ttagcagtat agaaacataa ttgactgaat
    10381 tctccatagg ttgggggaaa aggcacagag aagaaaccat agaaaaaact acaatcaaga
    10441 gcttcttcat atagtcctct tcctttgatt atatttagat attatatcac aatatgatag
    10501 gaatagtatg gcgctaataa cgactggata tgctttgtgg gtcaatattt agcaatctca
    10561 cgcagaccat aaggaggttc tatgcgcgtt agcaaaaaat ttggaatcgc tgtaggatgc
    10621 gtgctgctta ttctattgtt gtcgagctgt gtcgatgtcg agtacagaat ctcgatcaat
    10681 agcgaccata gcgccgatgt gttctatcag attctcatga atcccttgtt cgctagtttg
    10741 atgcaaggcg atgctgacca aggtccgtta gattcaatgc gtgaatactg ttacgaacct
    10801 tgtgtatttt tctaagagat ttgggaggaa gtatctcatg cataagcata agcaggtaca
    10861 tactccgctt ttctcttaag gggagttggg aaacttctaa gccatagctt tttatcaaga
    10921 agcgacaatc ctgctctgct taggaaagca cgttcttgga actgcttctc tctagcctta
    10981 caaatcgctt ttctttcaat accagctact tccgcatgcg tcataacgat atgccttcca
    11041 atcctgtaag gtttttcata attatgccaa cctaagtata cgacaaggcg attcagcata
    11101 ttcgcaacat ttcgcggaaa acaaactgtt tcgcgacgat gatcggcaag atcctttcta
    11161 agctctctgt cgagatagtt tgaggcaaag agaggattct ggaaggtacg tgggactttg
    11221 gaattgacgg tgagatgatg tatagagaag cgactagggt gtagtttgag cgctctagca
    11281 tattcacact tctcatctgt gatgaggatt agcggtgaag agtaagcact acagcgttcc
    11341 ctatataact gatctagaag ctctgagaag gagcgttcga gggcagcctt ttcaaagact
    11401 aatgatcgat agagtatgat ttttctgcgc ttttggctgt cggtgcagcg acctgagcgg
    11461 cgtaatgtgg catgagtgag acttagaata tactggctat gtgcac
//
LOCUS       DAOSOB010000060        11331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_5721_length_11331_cov_79.764278, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000060 DAOSOB010000000
VERSION     DAOSOB010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5721_length_11331_cov_79.764278"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3481)
                     /locus_tag="PLS88_06930"
     CDS             complement(<1..3481)
                     /locus_tag="PLS88_06930"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU07113.1"
                     /translation="MKKARIFAMMLVLVSISIGGVFGYDPPEGASLLGRIYGPWMMGA
                     GMSLTEKGDNTWNFHLNPASTSEEELPILALSYTGIADTSSGGPQGWGSAAMAAFSLP
                     QAYGVWNGSVRLFSAPGSMTSMPLGTFVTIGGGFSKKISDSLSVGASAWLAMGGNGSF
                     GWGLWSDIGIIKEFTDIGFLQNPRLGFVFSGIGKEFNYQVPPIGMSPGSIASTGFLPA
                     FSPGLGFSADLINDYNMRIRANVDLRAPSFGDLEGELGIGISYRNMLNLRISIAESLF
                     DIQNKTGRSLWPSITISGRIPLGSKDPQNKGVSAIVPALGFMPLYDSLDSFSIGAQLA
                     WRQPDKSSPEVKLELPPSVEGPPVAYISPNSDGKQDKLEIPLSVKDEQGIVAGWLFKV
                     EDRSSGKVVRTITEHSVLPDRIDSFSSLKQGFGYVKKNVQVPNILDWDGKDDEGNPVP
                     EGTYIVSFHAWDDLGNTNANYDSCMNVVVDRTAPTVNAWVLSSEQPLQGFDIMIFSPD
                     GDNSKDTISFRVQGSIEDNWKYEILNEGNVAVRTMEIKGRSAPRDFVWDGTDDRGKRV
                     PDGYYRFKLSTMDPAGNTGQKIIGISEDKSDWIIVDTSRPAVKVAANKKAFSPKKEGE
                     AGTALISFELESLDNLVTWKEFIQDQNGKTVWTSYGDGSTPPPRSFQFSGLGPSGEML
                     PDGQYRAGIEMKYRNGYSPLSYSESFILDATPPSAAIVLDDIRAIFSPDGDGSRDTLV
                     FSLSGSKEDVWNLSICNEAGESVYTRQYHDTLPEHFEWDGADSTGRRVKDDKYTLNLE
                     SVDIAGNEFKISYGPIVVDTRTPSASFVFNREVFSPNDDGVADILDVNLKIDSIDGLL
                     NWKLHVSSTIEGASDAEKTIRQITLLEGNKGSPPPTDFQFDGKGEGTLLKEGTYHFEL
                     SLEYENGWKTKKLSPEFVIDITAPKAQVSRSREFFNPRGEARQSQVIISQIGSKETLW
                     IGELRDSQGVLRCKWEFRDSQPSEILWDGRTAEGALLPDGIYAYRLFCIDLAGNSFAS
                     EPILIGIDTTLKQSSISADTMAISPNGDGIQDKMTLAISATAIESVQDWQLTILAGQE
                     KVMEWKGIGNVLRTIVWEGTSSARLSVPDGNYTAIFRAEYPNGDSTEASLGPLIVDRI
                     APQATVSLSRSIFS"
     gene            complement(3559..4536)
                     /locus_tag="PLS88_06935"
     CDS             complement(3559..4536)
                     /locus_tag="PLS88_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07114.1"
                     /translation="MYCAKPSIVVRNVLFAILFFIISFASDAMEPLPYRLFPALYRKE
                     NISRIDQNMSLANYQSSGGFLWERVPLVANNTILAYYGSPLSDKMGILGLYPKEKIAE
                     MLKEMASQYDQVNGKDGVIPAFYIIYGTCWPGGEIGYLDDKILLDYIRYAQSMGMLVF
                     IDHQIGKYSLRGAMDKILPFLRYPNVHLAIDPEWRTLSPMKEIGSITAAELNDAQNYM
                     DAYIRANDIPGIRMLVVHQFADKMIQDREEVISHRDRVILIHTADGFGAPKLKKATYQ
                     RNANAENMPIKGFKLFFKSDFPLAGFDMPLMSPVEVMQLEPRPSLVIYQ"
     gene            complement(4755..4946)
                     /gene="rpmB"
                     /locus_tag="PLS88_06940"
     CDS             complement(4755..4946)
                     /gene="rpmB"
                     /locus_tag="PLS88_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOU07115.1"
                     /translation="MSRVCEICGKGTLYGNNVSHSKNRTSRTWYPNLIKVRTIENGTV
                     RRIKVCARCLKSGKVEKYI"
     gene            complement(5057..6385)
                     /gene="ffh"
                     /locus_tag="PLS88_06945"
     CDS             complement(5057..6385)
                     /gene="ffh"
                     /locus_tag="PLS88_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOU07116.1"
                     /translation="MLEKISETFTDVFRTISGKSAISEKNVEDALDRIKIALLEADVN
                     VRIVRRFVNGVLEEARGEKVLRSVTPGQQFIKIVYDRMVALLGDEKQDLLLKGTDTQS
                     VILLLGLQGSGKTTTAAKLANFLKKRDRKVLLVACDLVRPAAVEQLAVLAGQVGVDIH
                     REELKDPIQVARNALTRAKREGHDVIIVDTAGRLQIDEPLMQELARIRDALSPVESLL
                     VADAMTGQSAVEIAKAFDERIGITGAILTKFDSDTRGGAALSLKSITGKPIKFIGVSE
                     RIDGLEPFYPDRIANRILGMGDIVSLVEKAQEVYDQKEALELEQKISKESFTLEDYLG
                     QIQKMKKMGSVKSMLEMIPGLAGQVDEDQIDLQEMKYEEAILLSMTKKERQNHLIIGP
                     SRRTRIAKGSGTSVAQVNRLLKKFEKSRSMMKKMVKNKGAMAKMFGSGAK"
     gene            complement(6427..6990)
                     /locus_tag="PLS88_06950"
     CDS             complement(6427..6990)
                     /locus_tag="PLS88_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU07117.1"
                     /translation="MTRDEAVVLWKLWNSDESLWHHALSTEAVMRHFARKYGEDAEYW
                     GIIGLLHDIDYEKFPEEHLKHAREILASAGFDDAFIRSVESHGWGICTQVEPKLLMEK
                     VLFAADELTGFIAACSYVRPSRSVLDLEVKSVKKKWGSAAFAAGVRRNVIEQGAQMLG
                     VPLDELIGETILALRGVASEIGLKGNL"
     gene            complement(7025..8899)
                     /locus_tag="PLS88_06955"
     CDS             complement(7025..8899)
                     /locus_tag="PLS88_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOU07118.1"
                     /translation="MENGVQTRFQRRIIVLVALLTLFAGVVLVRYGLLALEGPENNRL
                     SAEAPIERGRILDRNGKLLAFDIPKFNLAIRKNEIDPYRISKDLSSVAKSIGLQPESL
                     EQKIRESSQNFVYLAKRLDIDIAKPLQARIEKGELAGFVLEEINGRIYPEGRLASHLI
                     GFTGEGNTGLEGVEFKYDKELSSIGSDKLRGNDVYLTIDARLQYSLEGIARKAFEQNK
                     AESIFILAMDVNSGEVLAYVQMPDFDPNTFASFRESEREDRMSVYSYEPGSVFKIFSM
                     SSILDAGLITPRTKFNCDGAYQKVLPSGEKIIIKDLNSYGMLDLAGILAKSSNAGAGY
                     ASDRISDNEFYSRILSFGFTEKTGIGSPGENPGSLREPSKWSARTKPTVAIGQEIRVT
                     ALQMITASVAIANGGILLKPNTISRIEDANGNRIYSHEVIAKRRVIAPETSRAIMAAM
                     ESAASLEGTGWRAKVPDVRMAVKTGTAQMIDPKTKAYSDTDFIASTLGILPANNPKLA
                     IYVAIVKPKGESYLGGQIAAPVLRDAAEAAIGILGLPREKSLNANHAGIVVIQEPPLA
                     SIGANMPDLKGYSKRQLLPLLLRKDIEVQLEGDGYVVAQFPSPGTAVEAGTRIVLRLQ
                     "
     gene            complement(8906..9334)
                     /locus_tag="PLS88_06960"
     CDS             complement(8906..9334)
                     /locus_tag="PLS88_06960"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOU07119.1"
                     /translation="MKGSPSPKIKKFDSLMITILAIVVVAFLVRFFILDAAIVEGKSM
                     LPYYRNGEVVLIMKAAYGIRGLEGNYWMRWKLPAAGEVVVALSPTSHEIIIKRIGEVR
                     KQGEELAYFLIGYNPIESIDSRDFGLVQFDSIIGKVIPQR"
     gene            9422..10627
                     /locus_tag="PLS88_06965"
     CDS             9422..10627
                     /locus_tag="PLS88_06965"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU07120.1"
                     /translation="MKSVDHSENSILEADEILNKPLVSVLSGENFIQKPPLKKSKKAH
                     SIPSMQKQRPSSEPLFSPSTVKWNYNSSGEKASNDLQQFHRNPFKNLIKLPPRILLLI
                     AVLLTTSGAGVLAASFISHSHPSISLPDENSAQEALMAMLEPEPSSPPTDESALPPIP
                     SLLAEKTYTIRRGDTLQTIASRFGVREDTIISANNLSSKSQIQVGKTLKIPNMNGIYH
                     TVKKNESLSSISRTYGVDMTRIADANNMSSATIRIGERIFIPNAKLNASVLRNFYGET
                     FIWPVRGQISSPFGYRTNPFSGQRTFHAAIDIVVNRGTVVKATREGKVADTGYNAVFG
                     NYVIIRHADGYQSLYAHLDSILAKKGTSVNQGETIGRSGNTGQSTGPHLHFAIFRNGQ
                     AVDPRQYVK"
     gene            complement(10687..>11331)
                     /locus_tag="PLS88_06970"
     CDS             complement(10687..>11331)
                     /locus_tag="PLS88_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07121.1"
                     /translation="LTHATLRRSGRCTDSQKRKKTILYRSLVFEKAALERSFSELLDQ
                     LYRERCSAYSSPLILITDEKCEYARALKLHPSRFSIHHLTVNSKVPRTFQNPLFASNY
                     LDRELRKDLADHRRETVCFPRNVANMLNRLVVYLGWHNYEKPYRIGRDIVMTHAEVAG
                     IERRAICKAREKQFQERAFLSRAGLSLLEKKLWLRSFPTPLKRKAEYVPAYAYA"
BASE COUNT         3081 a         2730 c         2233 g         3287 t
ORIGIN      
        1 gagaaaatat cgagcgagaa agactaacag tcgcttgtgg cgctattcga tctacgataa
       61 gagggcctaa actagcttcc gtggagtctc cattggggta ttcggctcgg aagatagccg
      121 tatagttgcc gtcaggaaca gacaagcgag ctgagcttgt gccttcccaa acaatagtcc
      181 ggagcacatt tccgatacct ttccactcca taaccttctc ttgtccagca agtattgtaa
      241 gttgccaatc ctgaacgctt tctatcgctg ttgcgcttat ggcaagagtc atcttatcct
      301 gaatcccatc tccattcgga ctaatagcca tagtatcagc ggaaatagaa gactgtttta
      361 aagtagtatc gataccaatc aagataggct ctgatgcaaa actgttccca gccaaatcta
      421 tacaaaatag tcgataggca tagattccat cagggagtag agcaccttcg gctgttctgc
      481 catcccaaag gatttctgat ggctgactat ccctaaattc ccacttgcac ctcagcacac
      541 cctgactgtc gcgcaattct cctatccata gagtttcttt cgagccaatc tgagaaataa
      601 tcacttgcga ctgcctggcc tcacctcgag gattaaagaa ttctcgcgag cgcgatactt
      661 gagcttttgg tgcggtaata tcgataacaa attcagggct cagctttttt gtcttccagc
      721 cattttcgta ttctagcgag agttcaaagt gataagtgcc ttcttttaaa agtgtccctt
      781 ctcccttgcc atcgaattgg aaatcagtgg gaggagggct gcccttattg ccctcaagca
      841 atgtaatttg tctgattgtc ttttcagcat cagatgcgcc ttctattgta gaggaaacat
      901 gaagcttcca gttcaaaagc ccatcaatgc tgtcaatttt caaattcaca tcaagaatat
      961 ctgcgacacc atcatcattc gggctaaaga cttccctgtt gaacacaaaa gaggcgcttg
     1021 gcgttcttgt gtcaacaaca atcggtccat agcttatctt gaattcattg cccgcaatat
     1081 ctacactttc gaggtttagt gtatatttat catctttaac acggcggcct gtcgaatcgg
     1141 cgccatccca ttcaaaatgc tctggcagag tgtcgtgata ttgtcgagtg tagaccgact
     1201 caccagcttc attgcagatg ctaagattcc atacatcttc tttgcttcct gataagctaa
     1261 aaactagcgt atctctgctt ccatctccat cagggctgaa aatagctcga atatcatcaa
     1321 gaacaattgc ggcagatggt ggcgtcgcat ctagtataaa agattccgaa tagctgagag
     1381 gcgaatatcc atttcgatac ttcatctcaa tgcctgctct atactgtcca tccggaagca
     1441 tttctccaga aggtccaagt ccactaaact ggaaagatct cgggggtggt gtcgagccat
     1501 caccatagga ggtccagact gtcttgccat tctgatcttg aataaactct ttccaggtaa
     1561 cgaggttatc taaggattca agctcgaaag agattagtgc agtacccgct tctccttctt
     1621 tttttgggct aaaggccttc ttgttagctg cgacttttac tgcaggtctg ctggtgtcta
     1681 ctatgatcca atcggattta tcctcagaaa tgccaatgat tttttggcca gtgtttccag
     1741 caggatccat tgtagaaagc ttgaaacgat agtaaccatc ggggactctt ttgcctctat
     1801 catcagtgcc atcccaaaca aagtcgcgag gcgcacttct gccttttatt tccattgtcc
     1861 taacagctac attgccttca ttgagaattt cgtacttcca gttgtcttct atagaacctt
     1921 gaacgcggaa ggaaatcgta tctttgctat tatcgccatc agggctaaaa atcattatgt
     1981 caaaaccttg taagggctgt tcgctgctca atacccatgc attcactgtt ggcgctgttc
     2041 ggtccacaac aacattcatg caagagtcat aatttgcgtt tgtgtttcca agatcatccc
     2101 aagcatggaa actcacaata taggttccct cgggtactgg attgccttca tcatctttgc
     2161 catcccaatc taagatattc ggaacctgta cgttcttctt tacataacca aaaccctgct
     2221 ttagggaaga aaaggaatct atacgatcag gaagaacaga atgttctgta atagtcctta
     2281 ctaccttgcc agaggagcgg tcctcgacct tgaataacca accggctaca attccctgtt
     2341 cgtcttttac cgaaagagga atctctagct tgtcctgttt gccatcgcta ttgggtgaaa
     2401 tataggctac aggaggacct tctacagaag gcggaagctc gagcttcact tcgggagagc
     2461 ttttatcagg ctgtctccaa gcaagctgag cgccaataga gaatgaatct aagctatcat
     2521 aaagaggcat gaagccaagg gctggaacaa tagccgatac tcccttattc tggggatctt
     2581 tgctgccaag aggtatcctt cccgaaatgg tgatagaggg ccataaactc cgccccgttt
     2641 tattttgaat atcaaatagg ctttccgcta tggaaattcg aagattaagc atgtttcgat
     2701 aagaaattcc tattcccagc tcaccctcta aatctccaaa cgaaggcgcg cgcaaatcaa
     2761 cattggccct tatgcgcata ttgtaatcat ttataagatc tgcgctaaat ccgagacctg
     2821 ggctgaaggc ggggagaaag ccagtagatg caatgcttcc gggcgacatg cctattggcg
     2881 gtacctgata gttgaattct tttccaattc cgctaaaaac aaaacctaat cttggattct
     2941 gcaaaaatcc tatatcggta aattctttga taatcccaat atcggaccaa agcccccatc
     3001 caaagcttcc attccctccc attgcaagcc aagcggaagc tcccaccgac agactatctg
     3061 agattttttt cgagaatcca cccccgatag taacaaaggt cccaagaggc atgcttgtca
     3121 ttgaacctgg agcgctaaaa aggcgcacag aaccgttcca tactccataa gcttggggta
     3181 ggctgaaagc tgccattgct gcactacccc atccttgagg cccccctgaa gaggtgtcag
     3241 cgatgcctgt gtaggataaa gcgagaatag gaagttcttc ttccgatgta gaagccgggt
     3301 ttaaatgaaa attccatgtg ttgtcccctt tttcagtgag cgacattcca gcgcccatca
     3361 tccaaggacc gtaaatacgg ccaagcagcg atgctccctc gggcggatca tagccaaaga
     3421 caccacctat actaatagag accaatacaa gcatcattgc aaaaatccgg gcttttttca
     3481 tcgatatccc cttctctatt aaagaaacct tcagattcaa tctatcacag gaagagccca
     3541 acttcagggc ttgactcatc actgataaat gaccaggctt ggccttggct caagctgcat
     3601 aacttcgaca ggcgacatca gtggcatatc gaatcccgct agaggaaaat cagatttaaa
     3661 aaagagcttg aaacctttta tcggcatatt ctctgcgttc gcgtttcttt gataagtggc
     3721 tttcttgagc ttcggagcac cgaaaccatc cgcagtatga ataagaatga cacgatccct
     3781 gtgcgatatc acttcctcac gatcttgaat catcttgtct gcgaactgat gcacaacaag
     3841 catacgtata ccaggaatat cattcgccct gatatatgca tccatataat tctgagcatc
     3901 gttaagctca gccgcggtaa tagatcctat ttctttcatg gggctgagcg ttctccactc
     3961 cggatctatc gctagatgga catttggata tcgcaaaaat ggcaagatct tgtccatggc
     4021 tccccgaagg ctgtatttgc ctatctgatg atcgataaat acaagcatgc ccatagattg
     4081 agcatatctg atgtaatcca aaagtatttt atcgtctaga taacctattt ctcctcctgg
     4141 ccagcaagtt ccatagatta tatagaatgc tggaatcaca ccatccttgc catttacctg
     4201 gtcatactgt gatgccatct cctttaacat ttcagcgatc ttttctttcg gataaaggcc
     4261 aagtataccc attttatcgg aaagtggact gccataataa gcgagaatag tattgtttgc
     4321 cacaagaggt accctttccc acagaaagcc tccactggat tgataattgg caaggctcat
     4381 attctgatca atacgagaaa tgttttcttt tctatagaga gcaggaaaaa gcctataagg
     4441 aaggggttcc attgcatcgc ttgcaaagga aattataaag aagagaattg caaaaagtac
     4501 attgcgcact acgatggaag gctttgcaca atacattata aaccctttca atacacaact
     4561 attcattatt gtatcggcat caacttgagg cttctatagc cccacccaca gggagtctag
     4621 tctatctccg gagcatattt gtaaaccgct tatcaatata ccgtgggaat aaaaaaaccg
     4681 ccatattttg gcggcttttt aaataatcca ttacaaatcg gcaagcattt tgcctaaagc
     4741 cgattgagaa acaactaaat gtatttctcc acttttccag atttgagaca acgagcacat
     4801 acttttatgc ggcgcacagt tccattctca attgtgcgca ctttgatgag attcggatac
     4861 caggttcggc ttgtgcggtt ctttgaatgt gatacattat ttccgtaaag tgtacctttg
     4921 ccgcagattt cacacactct agacattgta gtctccatag aacatagtcg atgcaatata
     4981 ccgaaaatga cgaattatgt aaagtggtca ggatataatt agctcgttta aatatattcc
     5041 tttactctag cttgaattat ttagctccac tcccaaacat tttcgccatg gctcctttgt
     5101 tctttaccat ttttttcatc atcgatctgg atttttcaaa tttcttgagg agtctattga
     5161 cctgagctac agaagtacct gagccttttg cgattctagt tcgcctggag gggccaataa
     5221 tcagatgatt ctggcgttcc ttttttgtca tggatagaag tatagcctct tcatatttca
     5281 tctcctgaag atctatttgg tcctcatcaa cttgacctgc cagacctgga atcatttcga
     5341 gcatcgactt gacggagccc atctttttca ttttttgaat ctgacctaga taatcttcga
     5401 gtgtaaaact ttccttgctg attttttgtt cgagctcaag agcttccttt tggtcgtaga
     5461 cttcctgagc tttctccact agcgatacta tgtctcccat gcctagaatg cgattggcga
     5521 tcctatctgg gtaaaagggt tcaagtccat cgattcgttc gctgacacca atgaacttta
     5581 ttggcttgcc agtaattgat tttagtgaaa gcgcggctcc tccacgggta tctgaatcga
     5641 attttgtcag gatggcgcca gttattccaa ttctttcatc gaatgctttg gcaatttcga
     5701 ctgcggactg gccggtcata gcatctgcta caagaagact ctccactggc gaaagtgcat
     5761 cgcgaattct ggctaattct tgcattagag gctcatcgat ctgtagacgg cctgcagtat
     5821 cgacgatgat cacatcatga ccttcacgct ttgcccgtgt taaagcattg cgtgctacct
     5881 gtatcggatc tttaagttct tctctatgga tatcgacacc gacttgtcca gcaagcacgg
     5941 caagctgttc caccgctgcg ggtctcacaa gatcgcaggc tacgagcaat acttttcgat
     6001 ctctcttctt aagaaagttt gcaagtttcg ctgcggtagt agtctttcct gaaccctgaa
     6061 gacctaagag aagaattaca ctttgagtat cagtcccttt tagaagaagg tcttgctttt
     6121 catcgcctag aagagcgacc atgcggtcat agactatctt tataaactgc tgcccaggtg
     6181 tgacagaacg aagcaccttt tcccctctgg cttcttccaa cacaccattg acaaaacgac
     6241 gcacaattct cacatttacg tcggcttcaa ggagcgcaat tttaattcga tcgagggcat
     6301 cttcgacgtt tttctctgaa atagccgatt tgccacttat tgtcctgaat acatccgtaa
     6361 aggtctctga aatcttttca agcattgttt ctttccatag atatctatta ttgaattccc
     6421 tttgagctat agattccctt taagccctat ctccgaagcg acgcccctaa gagctagaat
     6481 tgtctcacca ataagctcgt cgaggggtac acccaacatc tgagctcctt gctctatgac
     6541 gtttctgcgc acaccagctg caaatgcagc agagccccat ttcttcttta cagattttac
     6601 ttccaagtca agcacgctcc gcgatggcct aacataggag caagcagcaa taaaaccggt
     6661 caattcatca gcagcaaaaa gcaccttttc cattaggagc ttaggttcga cttgtgtgca
     6721 tattccccag ccatggcttt ctacagatcg aataaatgca tcatcaaacc ctgctgatgc
     6781 aaggatctct cgtgcatgct tcaaatgttc ctcaggaaac ttctcgtaat cgatatcgtg
     6841 caaaagacct atgatccccc aatattctgc gtcctcacca tattttcgtg caaaatggcg
     6901 catcaccgct tcagttgaaa gagcatggtg ccacagagat tcatcactgt tccatagctt
     6961 ccataaaaca actgcttcat ctctagtcat tcttcatacc tcaagccaga aatgtcttga
     7021 aaaatcattg tagccgaagc actattcttg taccagcctc tacggctgta cccggagatg
     7081 gaaactgagc aaccacatat ccatctcctt caagttggac ttcgatgtcc tttcgcaaaa
     7141 gaagaggcaa caattgacgc tttgaatagc cttttagatc tggcatgttt gcaccaatcg
     7201 aggctaaagg aggctcctgg atcaccacta ttccagcatg attagcattt agactttttt
     7261 ctctcggcag gccaagaatg ccaatcgcag cttcagctgc atctcgcaaa acaggcgctg
     7321 caatttgacc tcctaaatac gattctcctt ttggctttac aatggcaaca tatattgcaa
     7381 gctttggatt atttgctggc aatattccca atgtactggc gataaaatcc gtatccgagt
     7441 aggctttggt ttttggatct atcatctgcg cagttccggt ctttacggcc atacgaacat
     7501 caggcacttt ggctcgccat cctgtcccct caaggcttgc agctgattcc atcgcagcca
     7561 ttattgctct agatgtctcg ggcgcaatga ctcgtcgctt agctattact tcgtgtgaat
     7621 aaatcctatt gccattagca tcctctatgc gagatatggt gttgggctta agtagaatac
     7681 ctccatttgc tatagcgacc gaggctgtta tcatctgcag tgccgttaca cgtatctcct
     7741 gtcctatcgc cacggttggt tttgttcttg cagaccactt tgaaggttct cttaagcttc
     7801 caggattctc gccaggggat ccaattcccg tcttttctgt aaaaccgaag cttaaaattc
     7861 gggaatagaa ctcgttatcc gaaatgcgat ctgaagcata accagctcca gcattgctgc
     7921 tcttagcaag aatacctgct agatccaaca ttccatagga atttaaatcc tttattataa
     7981 ttttttctcc agaaggaagg accttttggt atgcgccgtc gcagttgaat ttagtcctcg
     8041 gcgttattag accagcatcg agtatcgatg acatcgaaaa tatcttgaac acgcttcctg
     8101 gttcgtaaga ataaacagac attctgtctt cgcgctccga ttctctgaaa ctagcaaaag
     8161 tgtttggatc gaaatctggc atctgaacat aggcgagaac ttcgccgcta tttacatcca
     8221 ttgccaatat aaaaatggat tcggctttgt tttgctcgaa ggctttgcgt gcgattcctt
     8281 ccaatgaata ctgaagtcgt gcatctatgg tgagatagac atcgtttccg cgtagcttgt
     8341 cacttccaat actcgaaagc tctttgtcat atttaaattc gacgccttct agtccagtat
     8401 ttccttctcc tgtaaagcca atcaggtgtg aggctaatct cccttcagga tagattcggc
     8461 catttatttc ttcgagtaca aaaccagcaa gctctccttt ttcaattctt gcctgcagtg
     8521 gctttgctat atcgatatcc agcctcttgg cgagatatac aaaattttgg ctcgattccc
     8581 ttattttctg ctcaagagac tcaggctgta agccgattga ttttgcaaca ctactcagat
     8641 ctttggagat tctatatgga tcgatttcat tcttacgaat ggcaagattg aattttggaa
     8701 tgtcaaaagc caaaagcttt ccatttctat caagaatcct tcccctttca atgggtgctt
     8761 ctgctgaaag cctattattc tctggtcctt ccagagccag caagccatat cgaacaagaa
     8821 caacaccagc aaatagtgtc aacaaagcga caagcacaat tatccttctt tggaagcggg
     8881 tttgtacacc attctccatc tgcgcctacc tctgaggaat tacctttcct attattgaat
     8941 cgaattgtac aagtccgaaa tctcttgaat cgatcgattc aatcggattg tatcctatga
     9001 ggaaatacgc taactcctct ccttgttttc gaacttcccc tattcttttt attattatct
     9061 catgcgaagt gggtgataga gccacaacaa cctctcctgc cgctggtagc ttccatcgca
     9121 tccagtaatt cccttctaaa cctcgtattc cataagctgc cttcatgatc aataccacct
     9181 cgccgttcct ataataagga agcatggatt tcccttccac aatggctgca tcgagaatga
     9241 aaaacctaac caagaaggcc acaacaacaa tagcaagtat agtaatcatt aagctgtcga
     9301 actttttaat tttcggactg ggtgatccct tcattaatat ctcccttttg ctctcttgca
     9361 ttctagcttt cgacagctta aattaaatta tcggctaaaa tatgccgata caaaagaagc
     9421 tatgaaatct gtggatcact ctgagaattc tatcctcgaa gcagatgaga ttctcaataa
     9481 gcctcttgtt tctgtgctct ctggcgaaaa ttttatccaa aagcctccac taaaaaaatc
     9541 caaaaaagcg cattcaatac catcgatgca aaagcaaagg ccttctagtg agcctctttt
     9601 ctctccatca actgtcaagt ggaactacaa ttcttcagga gaaaaggctt ctaacgatct
     9661 acaacaattt catcgaaatc cattcaagaa tctaatcaag ctgccaccgc gcattctttt
     9721 attgattgcg gttttattga ccacaagtgg tgctggtgtg cttgcagcct ctttcatatc
     9781 acattctcat cctagcataa gccttccaga tgaaaactca gcgcaagaag cgcttatggc
     9841 aatgcttgaa cctgaaccat catctccacc taccgatgaa tcagctcttc caccgattcc
     9901 ttcgctccta gcggagaaaa cctatactat acgtcgcggt gacactctac agacaatcgc
     9961 aagcagattt ggtgttcgcg aagataccat aatctcagca aataatctga gctctaaaag
    10021 ccagattcaa gttggcaaaa ccctcaaaat acccaatatg aatggcatat atcacactgt
    10081 caaaaagaat gagagcctct cttctatttc cagaacctat ggagtagata tgacacgcat
    10141 cgctgatgcc aataatatgt cctcagcaac aattagaata ggagagcgaa ttttcatacc
    10201 aaatgccaaa ctcaacgcct cggttttgcg caacttttat ggtgaaactt ttatttggcc
    10261 tgttcgtggc cagatctctt caccatttgg atatcgcaca aatcccttta gcggacagcg
    10321 gacatttcac gcagcaatag atatcgtagt aaatagagga acagtcgtca aagcaacccg
    10381 tgagggcaag gttgcagaca cgggatataa tgctgtattt ggcaattatg ttattatccg
    10441 acatgctgat ggctatcagt cgctctatgc tcatttagat tcgatccttg ctaaaaaggg
    10501 aacaagcgta aatcaaggcg aaactatagg ccgctctggg aatacaggtc agagcactgg
    10561 acctcatctg cattttgcta tcttcagaaa tggtcaagct gtcgatccac gacaatatgt
    10621 gaaataaaaa cattcgatac tgttacgaac cttgtgtatt ttctaagaga tttggtagga
    10681 agtttttcat gcataagcat aagcaggtac atactccgct tttctcttaa ggggagttgg
    10741 gaaacttcta agccatagct ttttttcaag aagcgataat cctgctctgc ttagaaaagc
    10801 gcgttcttgg aactgcttct ctctagcctt acaaatcgct cttctttcaa taccagctac
    10861 ttccgcatgc gtcataacga tatcccttcc aatcctgtaa ggtttttcat aattatgcca
    10921 gcctaagtat acgacaaggc gattcagcat attcgcaaca tttctcggaa aacaaactgt
    10981 ttcgcgacga tgatcagcaa gatcctttct aagctctctg tcgagatagt ttgaggcaaa
    11041 gagaggattc tggaaggtac gtgggacttt ggaatttacg gtgagatgat gtatagagaa
    11101 gcgactaggg tgtagtttga gcgctcgagc atattcacac ttctcatctg tgatgaggat
    11161 tagcggtgaa gagtaagcac tacagcgttc cctatataac tgatctagaa gctctgagaa
    11221 ggagcgttcg agtgcagcct tttcaaagac taatgagcga tagagtatag tttttttgcg
    11281 cttttggctg tcggtgcagc gacctgagcg gcgtaatgtg gcatgagtga g
//
LOCUS       DAOSOB010000061        10815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_6074_length_10815_cov_50.709015, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000061 DAOSOB010000000
VERSION     DAOSOB010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6074_length_10815_cov_50.709015"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="PLS88_06975"
     CDS             <1..1553
                     /locus_tag="PLS88_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOU07122.1"
                     /translation="QEDIPCEGMYFSSGYAKAENGERYTFVWNEKKFPHAEEFIRNLR
                     NRGYRICCNVKPGILLAHPWYDRLAKDDVFIPDNDGSPLISYYWGNSASFIDFSRKEG
                     FDWWVNALKKYILEKGISGVWNDNNEFEIEDKALPIQDVKNILPIKMAEASFKALRSF
                     YPGKRPWLISRSGYAGLQRYARTWTGDNVSSYDTFRFNIAMGMNLGLSGLPFYGHDIG
                     GFVGPIPDEELLLRWCQSAVFQPRFVMHSWKPDGSITEPWLYPNRLSDIRNFIRIRYR
                     FLPYIYDLAIRASETGIPMESPVALEFPKDISLQLDSLDRMAGDAILIPAPPEQGKDE
                     VSMKLPDGINWFDPIPQIIYKGGETLRFHYDLGEIRYFFRCGTVIPTFYKIEAIGKSL
                     LDGYEFIVIPPAEKSDIDLLPIACIHSEDDGESDFILGSFWRWKMEYTMEEENCYCLN
                     ITQTESVDDKHNSSVFRKKWRFLVPDGFMLYDGNEVEMGNSVAFDFDKAPKRLSLRIK
                     GAYRNKQG"
     gene            1571..2560
                     /locus_tag="PLS88_06980"
     CDS             1571..2560
                     /locus_tag="PLS88_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU07123.1"
                     /translation="MVTIRDVAEHAGVSVSTVSHALSGKRPISQVTRQRIFQAIEQLG
                     YEPNPAARALRTKSTGVIGFFAFDITEVFAARIIHGVERIVREKNCFLLFTSGVGFNY
                     DLAHGIDFLRKRRVDGIIIAYGVRQEISPKTFDLSNIPLVTINTFVDPTIPSLHPDDF
                     GGGKEAAMHLVSKGARHPAMIAGPENRFASKERLLGFEKGLEECGIPFDASERVVHGD
                     FGAESGSYCFDALLKACPDLDAVFCANDYMAAGAINRALASGISIPRDLHIIGYDDRE
                     FASFWPIPITTFAQPLEYMGELGATMLFERIDGRNPEPSMVKLAPTLIARRSS"
     gene            2682..4145
                     /locus_tag="PLS88_06985"
     CDS             2682..4145
                     /locus_tag="PLS88_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOU07124.1"
                     /translation="MANHASCFFDSDNEKNSFPFADNDNPLDLVIAYIGGGSRGWAWT
                     LMSDLALEGSLGGIVRLYDIDKEAAEINARIGNTLRERDDVLGKWRYEIAEDLESALR
                     GADFVIASILPGTFAEMASDVHAPEKYGIYQSVGDTTGPGGIVRALRTIPMYEVIGMA
                     IREFCPKAWVVNYTNPMALCLRTLYEIFPDIKAFGCCHEVFGTRRLLAAMVRERLGAE
                     GIVDAKNATMQQIEVNVLGINHFTWIDRASFGTVDLIPHYRAFVEEHFESGYEGAESE
                     SWLNSYFVSAERVKFDLFKRYGLIAAAGDRHLAEFMPGRMYLRDPETVRSWKFSLTPV
                     SWRIENAEKLKKKAMSYAEGKEQFELKHSGEEGVKLIKALCGLGSFVSNVNLPNHGQI
                     KGLPLGAVVETNAIFWCDCVQPVDAGRLPSQIEALVVPHAIAQDIVLHASMRRDAWAA
                     FPAFLHDPLMTATPSEAEALFASMLSATAKYLPDYSL"
     gene            complement(4160..5416)
                     /locus_tag="PLS88_06990"
     CDS             complement(4160..5416)
                     /locus_tag="PLS88_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU07125.1"
                     /translation="MKKTGLALLIILMSAVMVFGQQQITIRWAYWGSESRVKRSQEAI
                     NLFEAANPGIKVNPEISGGTGDHFNKVDTQLAGGSGPDIIQMGGNINDYVKRGVLLPL
                     EKYSGTILNTAVIDPSAIESGTIDGHLYGVSTGVTMPALVYNKSLIQRAGAPLPKVSM
                     TYEEFRNYLVTLKSKLPAGVYPMQDIGALSSNSTPFGYWTRYNGTPLYDAATKTTAVK
                     PADAQKYLELFKDYRNNGLVPPADIAAGYAETNADTSMVIAGKAAIGFIWTNQLAGYQ
                     AATTDELCLMEFPGAAATKALWQAPSQFYTVNKNSKYPEAAVKFINFLVNSPEAAKVL
                     GNDRGASASATARAAGAADINDRKVLEYLDVAGPHSSKETDHVPNDTEFNSTLFLIYQ
                     KVAFGQISTAAGGQQIYDLIVRLIKK"
     gene            complement(5447..6295)
                     /locus_tag="PLS88_06995"
     CDS             complement(5447..6295)
                     /locus_tag="PLS88_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU07126.1"
                     /translation="MKKHTRNLINSIITNAFIILLGVCMLYPVVWLIAASFKESNTIF
                     SDPGLWPKAFTLQNYVKGWQGIGIVGFGTFFKNSFIISGLCVIANVIFCSLTAYAFAR
                     LKFAGRNFWFAVMMVTMMLPTHVTTIPRYIMFRKFGWIDTFLPLVIPKFFATDAFFIF
                     LLVQFIRSLPKELDESALIDGCGKFGIYTQIVMPLTKPALITTVIFTFLWTWDDFFNQ
                     LLYLNSPSKYTVPMGLRLFLDSSGISSWGPMFAMSVLSLLPCFILFFSLQKYFVQGIA
                     TTGIKG"
     gene            complement(6292..7218)
                     /locus_tag="PLS88_07000"
     CDS             complement(6292..7218)
                     /locus_tag="PLS88_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU07127.1"
                     /translation="MNKRARKKAIRENLTSYAFLIPWLLGFFVLTLYPMIYSFFLSFT
                     EFNILQPPKWIGLRNYFVMFIGNEQFPRDERFLNSLLVTFKYVFISVPLKLIFALGVA
                     MLMNRDLKLIPFYRTLYYIPTLLGGSVAIAALWRRLFAGDGVLNTILRSAFGLNPPAW
                     ISNPKYALYTLILLSVWQFGSPMIIFLAGLKQIPREYYEASAVDGAGKMRQFFSITLP
                     CLSPIIFFNLVMQMINAFQSFTQAYIVSGGQGGPLDSTMFYSLYLYIKGFAFSEMGYA
                     SAMAWVLLIIIAVLTALAFKLSGSLVSYGSGE"
     gene            7677..8570
                     /locus_tag="PLS88_07005"
     CDS             7677..8570
                     /locus_tag="PLS88_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOU07128.1"
                     /translation="MKRKDIQIRDPFVLPLQDEKRYCLYGTTDTDPWKAPGIGFDAYI
                     SENLEEWEGPYPIFRPLLGFWASHNFWAPEVYKYRESYYMFASFKSPEHERATQILRA
                     TVPFGPFLVHSNEPVTPSGWECLDGTFWEEEGQPWLVFSREWIEVNDGEIWAAPLSID
                     LDRLEAEPILLFRGSDASWTKKMKRRDGSGLEDARVTDGPFLYRSKSGTLFMLWSSIG
                     ESGYAMGYARSQSGKLIGPWLQESKPLVEGGRGHGMIFCGLDEKTYLAYHFPNETPYE
                     RFHYREIELSDSNIVLIGEEL"
     gene            8574..10622
                     /locus_tag="PLS88_07010"
     CDS             8574..10622
                     /locus_tag="PLS88_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07129.1"
                     /translation="MAIDRRSLVRRHNPVLRSYDPFSPLSLGNGEFAFTVDITGLQSL
                     VSNIPGITPLCTMSQWGFHSYPEREDCSRDEKRLRLSYFDPIRKTGGYMTDPTGQEEL
                     FNELRMNPHRINLARVGFVFEGKELKFQYISDIEQILDLWKGELHSRFSYKDQPVDVY
                     TICDPEVDALGIHVFSPLLDQGRLAVRIAFPYGSHEIDASDWNAEKRHSTIVLEHGKN
                     SRQENGESLSLERILDSEGYFVQIQAGATGDAYIHQESKHEFIIAGVGPQLDLVAQFS
                     PQKILEPPLSFRSISDRVARHWEEFWSTGGAIELIESRDERAMELERRIILSRYLTAI
                     QCDGSYPPSETGLTCNSWYGKFHLEMHYWHAAHFIVWGKPELFERSFEWYRSILPSAK
                     KRAQEQGYIGARWPKMTDPSGRDSPSPIGPLLCWQQPHPLMYIELLRRAYPNRQILYE
                     YRDIIEATADFMASFAQWNESKQRYDLGPPLIPAQENHRPEDTLNPPFEVEYWRWSLF
                     AAIYFFEMLDLPVNSKWREVASRLALLPVDKEKHAYLAHENCPKTYDQFASDHPEMLF
                     ALGMLPGPSVDKTIMSNTLDMVLKTWNLESLWGWDFPAMAMTAFRLGREKDAIDLLLM
                     QTPKNSYRANGHNPQLPRTDLPVYLPGNGALLLAMSLMAQDWEDEDWEMQAEGLLPIP
                     "
BASE COUNT         3014 a         2303 c         2579 g         2919 t
ORIGIN      
        1 aacaagaaga tataccctgc gaaggcatgt atttttcttc aggctatgca aaagcggaaa
       61 atggcgagcg ctatactttt gtatggaatg aaaaaaaatt cccgcatgcg gaagaattta
      121 ttcggaatct cagaaacaga ggatatcgaa tctgctgcaa tgtgaaacca ggcatattgt
      181 tggctcatcc atggtatgac cggcttgcta aggatgatgt ttttatacct gataatgatg
      241 gttctcccct aataagctat tattggggca atagcgcatc cttcatagat ttttctcgaa
      301 aagaaggttt tgattggtgg gtcaatgcgc ttaaaaaata tatcctcgaa aaaggaatct
      361 ctggagtatg gaatgacaat aacgaattcg aaatcgagga taaggctctc ccgattcagg
      421 atgtcaagaa tatattgcca ataaagatgg cagaagcatc ttttaaggca ttaaggtctt
      481 tttatcctgg caaaaggcct tggctgatca gtcgctctgg atatgcgggg ctccagcgct
      541 atgcccgtac atggactggg gacaatgtat ctagctatga tacatttcgc ttcaatattg
      601 caatgggaat gaatttgggt cttagtggtc taccatttta tggacatgac attggaggct
      661 ttgtcggccc aataccagat gaagaattgc tgctgcgttg gtgccaaagt gccgtttttc
      721 agccgcggtt tgttatgcac tcttggaaac ctgatggaag cataaccgag ccatggctct
      781 atccaaatcg actttcagat atccgcaatt tcatacgaat acgatataga tttttgcctt
      841 atatctatga tctagcaatt cgtgcaagcg aaaccggcat cccaatggaa agccctgttg
      901 cgctagaatt tccaaaggat atttcacttc aattagatag cctagatagg atggctggag
      961 atgccatctt gatacccgcc ccacccgaac aaggcaaaga cgaagtatcg atgaagcttc
     1021 ccgatgggat aaactggttc gatccaattc ctcaaatcat atataaaggc ggggagactt
     1081 tgcgtttcca ttatgatcta ggcgagatta gatacttttt cagatgcggt actgtaatcc
     1141 ctacgtttta caaaattgag gcaattggca aatccctttt agatggctat gaattcattg
     1201 ttatccctcc cgcagaaaaa agcgacatcg acttactgcc tattgcttgc atccatagcg
     1261 aggatgatgg agagagcgat tttattctcg ggagcttctg gcgatggaag atggaataca
     1321 caatggaaga agaaaattgt tattgtctca atataacgca gaccgaaagc gtcgatgaca
     1381 aacataattc ctcggtcttt agaaagaaat ggcgattttt ggtgcccgat ggtttcatgc
     1441 tatatgacgg gaatgaagtc gaaatgggca atagtgtagc attcgatttt gacaaagccc
     1501 caaagcgact ctctcttaga atcaagggag cctatcgcaa taaacagggc taagatgata
     1561 ttctagatta atggtaacga ttcgagatgt cgcagaacat gcaggagtat cggtttcgac
     1621 ggtgagccat gcgcttagcg gaaaacgccc cataagccag gtaacaagac agagaatatt
     1681 tcaagcaatt gagcaactcg gttacgaacc caaccctgcc gcccgtgctc ttcgaacaaa
     1741 gagcacgggg gttataggtt tttttgcttt tgatattaca gaagtatttg ccgcaagaat
     1801 aattcatggt gttgaacgaa tagttcgcga gaagaattgt tttctgcttt ttacaagcgg
     1861 cgttggattc aattacgatt tggctcatgg tatagatttt ttacgcaaac gaagggtcga
     1921 tggcatcatt attgcctatg gtgtacgtca agaaataagc ccgaaaactt tcgatctttc
     1981 caatatacct ttggtgacaa taaacacttt cgttgatcct accattccat ctctccatcc
     2041 agacgatttc gggggtggga aggaggctgc gatgcatttg gtttctaaag gcgctcggca
     2101 tcctgccatg atagctggtc ctgaaaacag gtttgcaagc aaagagcgcc ttttgggttt
     2161 cgaaaaaggg ctggaagaat gtggtattcc tttcgatgcc tcagaaaggg tagtacatgg
     2221 ggactttggt gcagaatccg gctcttattg ctttgatgcc ttattgaagg cttgtcccga
     2281 tctggatgca gtcttttgcg caaatgacta tatggcagca ggagctataa accgcgctct
     2341 cgcttctgga atctcaattc ctcgggatct acatataatt ggctatgatg atcgtgaatt
     2401 tgcctctttt tggccaattc ctatcactac attcgcacag cctcttgaat acatgggaga
     2461 actcggtgcg accatgcttt tcgaacgcat cgatggtcgc aatccagagc caagcatggt
     2521 caaactggcg ccaactctta ttgctcgtcg atcttcatag agctggatta acttagtggc
     2581 gcgagtttta tccacgatta tttttagctt atccttttca tttaggtcta aatatagtat
     2641 tgttgggcaa caagatctga gttctatcca aggaataaaa aatggcaaat catgcttcct
     2701 gctttttcga ttctgataat gaaaaaaaca gctttccttt tgcggacaac gataatccat
     2761 tagatctcgt catagcttat atcggtggag gctcgcgtgg ttgggcttgg accctgatga
     2821 gcgatctcgc tttggaagga tcgctcggag gaatcgttcg gctctacgat atcgataaag
     2881 aagcagccga gataaatgcg agaattggaa atactcttcg agagagagat gatgtgcttg
     2941 gaaagtggcg atacgaaata gcagaagatc ttgagagcgc cttacgcggt gcggattttg
     3001 taatcgcttc tattcttcct gggacttttg ccgaaatggc ttcagatgtt catgcgcccg
     3061 aaaaatatgg aatttaccaa tctgtaggcg ataccacagg accaggcggg attgtgcgag
     3121 cgctcaggac tatcccaatg tatgaagtga ttggaatggc gatacgcgaa ttctgtccca
     3181 aagcatgggt tgtcaattat acaaatccga tggctctttg cctccgcaca ctatacgaaa
     3241 tcttcccaga tatcaaggct tttggctgtt gccatgaagt attcgggaca cgtaggctat
     3301 tggctgcaat ggttcgcgaa cgccttggag ctgagggtat agtggacgct aagaatgcca
     3361 ctatgcagca gatcgaagtc aatgtgcttg gaataaatca tttcacatgg atagatcggg
     3421 catcgttcgg cacagtggat ctcatacctc actatagagc ttttgtagaa gagcacttcg
     3481 aatctggata cgaaggggca gagagcgaga gctggctcaa ttcctatttt gtatccgcag
     3541 agcgtgttaa attcgatcta tttaagcgtt atggtctaat tgcagcagct ggcgatagac
     3601 atcttgccga attcatgcca ggcagaatgt atctacgaga tccagagacc gttcgatcat
     3661 ggaaattttc tttgacacct gtatcatggc gtatagaaaa tgcggagaag ctcaagaaaa
     3721 aagcgatgag ctacgctgaa ggcaaagaac aattcgaact gaagcattct ggagaagaag
     3781 gcgtaaaact cataaaagct ctctgcggac taggttcatt tgtctcgaat gtcaatctgc
     3841 caaatcatgg ccagataaaa gggcttccat tgggagcggt cgtagagacc aacgctattt
     3901 tttggtgcga ctgtgttcag cctgtagatg cgggaagact tccctctcaa atcgaagccc
     3961 ttgttgtgcc acacgccatt gctcaagata tagttttgca cgcttccatg cgccgtgatg
     4021 cttgggcggc atttcctgca ttcttgcatg atcctttaat gacggctaca ccttctgaag
     4081 ccgaggctct ttttgcaagc atgctaagcg cgacagcgaa atatctacca gattactcgc
     4141 tatagaaagc aattgcggct catttcttta taagccttac aattagatca tagatttgct
     4201 gtccgccagc tgccgtagat atctggccaa acgccacctt ttggtagatt aggaacaatg
     4261 tgctgttgaa ttcggtatca ttgggcacat gatctgtttc tttcgaagag tgtggccctg
     4321 ctacgtccag gtattctaga accttcctat cattgatgtc ggctgctcca gctgctcttg
     4381 cggttgctga ggctgaggca ccgcgatcat tgcctaggac tttagctgct tctgggctat
     4441 tcacaaggaa gttgatgaac tttacagcgg cctctggata cttggaattc ttgttcactg
     4501 tgtagaactg gctaggagcc tgccatagcg ctttagtagc cgctgcacct ggaaattcca
     4561 tcagacacag ttcatcggtc gtggctgcct gataacccgc gagctggttc gtccaaataa
     4621 accctatggc agccttgccg gctataacca tcgatgtgtc ggcgtttgtc tctgcatagc
     4681 ccgcggcgat atcagctgga ggaacgagcc cattgttgcg gtaatccttg aagagctcga
     4741 gatacttctg tgcgtccgcc ggttttaccg cagtggtctt ggttgctgca tcgtatagag
     4801 gagtgccatt gtagcgagtc caatatccaa aaggagtgga gttagaggaa agagctccaa
     4861 tatcctgcat tggatatact ccagccggaa gtttcgattt gagcgtcaca agataattgc
     4921 gaaattcctc ataagtcatg gagacctttg gcagaggtgc accagccctt tgtatgagcg
     4981 atttattgta gacaagcgct ggcattgtta ctcctgtgga gaccccatac agatgtccat
     5041 cgatagtgcc ggattctatg gcgcttggat cgatcaccgc tgtgttgagg atagttcccg
     5101 aatatttttc cagaggaaga agcactcctc ttttgacata gtcattgatg ttaccgccca
     5161 tctgaattat gtcaggaccg ctcccgccag caagctgcgt atcgacctta ttgaagtgat
     5221 ctcctgttcc gcccgagatt tcggggttca ctttgatacc cggatttgct gcttcaaaga
     5281 ggtttatagc ttcctgactt cgttttactc gagattcgct tccccagtaa gcccaacgaa
     5341 ttgttatctg ctgttgcccg aataccatga cagcgctcat tagaataatg agtagtgcca
     5401 aacccgtttt tttcatgatc tacctcctat cgaaaagata taatgcctat cccttaatac
     5461 ccgttgtagc aattccctgc acaaagtact tctggagcga gaaaaagaga ataaagcagg
     5521 gaaggagaga aagaaccgac atggcgaaca taggccccca agatgagata ccagaggaat
     5581 caaggaagag cctgagcccc atgggaactg tgtatttaga aggggaatta aggtaaagga
     5641 gctgattgaa gaagtcatcc caagtccaca agaaagtgaa gataacagtt gttatcaagg
     5701 ctggtttggt gagtggcatg acaatctgtg tgtagatgcc gaatttgccg caaccatcta
     5761 tgagagcaga ctcatcgagc tctttgggaa gacttcgaat aaattgcact agcagaaaga
     5821 tgaaaaatgc atctgtagca aagaatttgg gaataacgag tggcaggaaa gtatctatcc
     5881 atccaaattt tcgaaacatg atatatcgtg ggattgtagt cacatgcgtt ggcaacatca
     5941 tggtaaccat catcacggca aaccaaaaat ttcttcctgc aaatttcagc cgcgcaaagg
     6001 cataggcagt gagggaacag aatatgacat ttgcaattac gcataacccg gatatgatga
     6061 atgaattctt gaagaaagtg ccaaatccca cgatgccaat tccttgccag cccttgacat
     6121 aattctgcag agtgaaggcc tttggccata atccaggatc gctgaagatt gtattgctct
     6181 ctttgaatga tgcggcgatt aaccagacaa caggatatag catgcataca ccgagcaaga
     6241 taatgaaggc atttgtgatg atagagttta tcaagttcct agtgtgcttt ttcattctcc
     6301 gctcccatag ctgacaagtg aacctgataa tttgaaggca agggctgtaa gaactgcaat
     6361 gatgatgagc aacacccacg ccatggcgga tgcatagccc atctcggaga atgcaaaacc
     6421 ctttatgtaa agatagagac tatagaacat cgtcgaatcc agcggcccgc cttgtccgcc
     6481 tgagacaata taggcttgtg taaaggactg aaaagcattg atcatttgca taacaaggtt
     6541 gaaaaaaatg attggcgata ggcagggcag ggtaatagag aagaattgtc gcattttgcc
     6601 agctccatct accgccgaag cctcatagta ttctcttggt atctgcttaa gacctgcgag
     6661 gaaaataatc ataggagagc cgaattgcca taccgatagc agaatcaggg tatatagagc
     6721 atatttagga ttcgatatcc aagccggtgg attgaggccg aaagcgctgc gcaagattgt
     6781 attcaatact ccatctccag caaagagcct tctccataga gcagcaatgg cgacggagcc
     6841 tcctagtagt gtaggaatat agtagagagt tcgatagaag gggatgagtt tgaggtctcg
     6901 gttcatcagc atagctacgc ccaacgcaaa tatcaatttg agagggacag aaatgaacac
     6961 atatttgaac gtaactagaa gagaattgag aaatcgctca tccctcggaa actgctcgtt
     7021 tcctataaac attacaaagt aattgcgtag gccaatccat ttaggaggct gaaggatatt
     7081 gaattcggtg aaagacagaa aaaacgaata tatcattggg tagagagtga gaacgaaaaa
     7141 gccgagcagc catggaatga ggaaggcata cgatgtcagg ttttcgcgga tagccttctt
     7201 cctagctctc ttgttcattt ttttcttccc tggtttccaa ctccaactct acaaacccta
     7261 catacctata ctaggtaccc atagataact ataaaactca tcaataaaaa cgcatttata
     7321 ttattaaaac tctttcggca aaataccatg taaaatattg acaatagata tctattattg
     7381 atatttcaag gcaacatcaa atgttgaaaa ctagcgtctt atatatataa aaatatgtat
     7441 attcagctta agaatctttt tgacgaatgc aaaatgaaaa attgtatttt gaagctaaca
     7501 tgattatttc ttgtattgac aaagaactct tctctttagg ctattttggt tcactaaaag
     7561 agaaaaagat ggaaagatcc gaatatcgga tagaattgcc gtccaaaaac atatctgtta
     7621 ttatgctatt atctagggaa tttagactta atgcgcaaca tataaaagag aaagccatga
     7681 agagaaagga tatccaaata cgcgatccat tcgtgctgcc tttacaagat gagaagcgat
     7741 attgcctata tgggactacc gatactgacc cctggaaggc gccaggtatc ggcttcgacg
     7801 catacattag cgaaaacctc gaagaatggg aggggccata tcctattttt agacctttgt
     7861 tgggtttttg ggcaagccat aatttttggg cgccagaagt atacaagtat agagaatcat
     7921 actacatgtt tgccagtttc aagtcgcctg aacatgagcg ggcaacgcaa attttacgcg
     7981 caactgttcc ctttggacca tttctagtgc attcaaatga gcctgtgact ccatcgggct
     8041 gggaatgcct agatggaact ttctgggaag aagaaggtca gccttggctg gttttttctc
     8101 gagagtggat cgaggtaaat gatggcgaaa tttgggcagc gcctctttcg atcgatctcg
     8161 ataggctaga agccgaacct attttgcttt tccgaggttc ggacgcatct tggacaaaga
     8221 aaatgaaaag acgcgatgga tcaggcctag aagatgcccg agtcaccgat ggacctttcc
     8281 tctatcgttc aaagagtgga acgctattta tgctctggtc tagtattggg gaatccggct
     8341 atgcaatggg atatgctcga tcccaatcag gaaaactcat tggcccatgg cttcaagaat
     8401 cgaagcccct tgtcgaagga ggacgtggcc atggcatgat tttttgcggt ctcgatgaga
     8461 aaacgtactt ggcttaccat ttcccaaatg aaactcctta tgagcgcttt cattatcgag
     8521 aaatagaact atcggattca aatattgtgc ttattggcga ggagttatag ttcatggcca
     8581 tagaccgacg ttctcttgtt cgtagacata atccagttct tcgcagctat gatccattct
     8641 ctcctctatc gttaggcaat ggcgaattcg cctttaccgt ggatatcacc ggcctacaga
     8701 gtctagtttc caatatacct ggcataacgc ccctttgcac catgtcacaa tggggattcc
     8761 attcttatcc ggaacgcgaa gattgctctc gcgatgaaaa aaggctcagg ctgagctact
     8821 tcgatccaat tagaaaaact ggcggctata tgaccgatcc aacaggtcaa gaagagttat
     8881 tcaatgagct tagaatgaat ccgcacagga ttaatctcgc tagagtcggt tttgtctttg
     8941 aggggaaaga gctgaaattt caatatatat ctgatatcga acaaattctc gatctatgga
     9001 aaggggagct gcatagccga ttttcctata aggatcagcc tgtcgatgtc tatactatct
     9061 gcgatcctga agtggatgca ttgggtatcc atgttttttc cccgcttctt gaccagggtc
     9121 gtcttgctgt gcgcatcgcc tttccttatg gttcgcacga aatcgatgct tccgattgga
     9181 atgccgaaaa gcgccatagt accattgttt tggaacatgg caagaattca aggcaagaga
     9241 acggcgaatc gctttcgctc gagagaattc tagatagcga aggttatttt gttcaaattc
     9301 aggctggcgc aacaggcgat gcatatatcc atcaggaaag caagcatgaa tttattattg
     9361 cgggtgtagg cccacagctc gatctagtag cgcaattctc tccacagaaa atccttgagc
     9421 ccccattgtc atttcgttct ataagcgaca gggttgctcg acactgggag gaattctggt
     9481 cgaccggtgg agcgatagag ctcatcgaga gccgagacga gagggcaatg gagctcgagc
     9541 ggaggataat tctttcccga tatctcaccg cgatccagtg cgatggatca tatccgcctt
     9601 cagagacggg gcttacatgt aatagctggt atggcaaatt tcatctggag atgcattact
     9661 ggcatgctgc gcacttcatc gtatggggaa aacccgagtt attcgagcga agcttcgaat
     9721 ggtatagatc tatcctgcct tccgcaaaaa agcgagcgca agaacaaggg tatataggcg
     9781 ctcgatggcc aaagatgacc gatccatcgg gaagagacag tccctcgcca attgggccgc
     9841 ttctttgctg gcagcagcct catccactta tgtatatcga gctcttaaga agggcttatc
     9901 cgaatcgaca gatcctttat gaatataggg atatcatcga agcaacggct gacttcatgg
     9961 ctagctttgc ccaatggaac gagtctaaac agcgctatga tcttgggcct cctctaatcc
    10021 ctgcgcagga aaatcatagg ccggaggata cactcaatcc gcccttcgag gtggaatact
    10081 ggcgttggag tttatttgcg gcgatttatt ttttcgagat gttggatctg cctgtaaact
    10141 cgaaatggag agaagtggcg tctaggctag ctttgcttcc agtggataag gagaagcatg
    10201 catatcttgc acacgagaac tgtcccaaga cctacgatca atttgcatcc gaccacccgg
    10261 agatgctttt tgccctgggg atgctgccag gcccttcggt agacaagacc ataatgtcca
    10321 atactctgga tatggttttg aagacctgga atttagaatc cctttggggc tgggattttc
    10381 ccgcaatggc catgaccgct tttcgcctag gaagggagaa ggacgcaatc gatcttcttc
    10441 ttatgcaaac gcctaagaac agctatagag cgaatggcca caatccccag ctgccgcgta
    10501 ccgacctacc cgtgtatctg ccgggtaacg gtgcgcttct attggctatg tcgctgatgg
    10561 cccaggattg ggaggatgaa gattgggaaa tgcaggctga ggggctattg ccgattcctt
    10621 aaagtattcg ttactgctac gaaccttgta taagaatttg aagcataaag tagggaagag
    10681 agcgataaag agaatgtatg gaggtaagca tgcattcttt tcctttctgc ccaaatccag
    10741 cctgtctcta tcatgaggca gctccaaaag gtcgctggtt tttaaggcta ggcttctatc
    10801 ataccaaatg ttttg
//
LOCUS       DAOSOB010000062         9785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_6932_length_9785_cov_83.931552, whole genome shotgun sequence.
ACCESSION   DAOSOB010000062 DAOSOB010000000
VERSION     DAOSOB010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6932_length_9785_cov_83.931552"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..396
                     /gene="rpsL"
                     /locus_tag="PLS88_07015"
     CDS             22..396
                     /gene="rpsL"
                     /locus_tag="PLS88_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012538144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOU07130.1"
                     /translation="MPTINQLIRNGRKQTQWKTKSPALMGCPQRRGVCTRVMTVTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGIGHNLQEHSAVLVRGGRVKDLPGVRYHIIRGTK
                     DALGVEDRKQGRSKYGAKKPKA"
     gene            409..879
                     /gene="rpsG"
                     /locus_tag="PLS88_07020"
     CDS             409..879
                     /gene="rpsG"
                     /locus_tag="PLS88_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOU07131.1"
                     /translation="MGRKKKAIDRGHAPDVKYNSVVISRFICRMMWEGKKSVCTGIMY
                     DALDLLQQRSGQPALDAFTKAIENVKPSIEVKSRRVGGATYQVPIEIRESRREALAMR
                     WIIQAARARTGKSMPECLADEFFDAFNNTGAAIKKKDDMHKMAEANKAFAHYRW"
     assembly_gap    976..1075
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1185..3299
                     /locus_tag="PLS88_07025"
     CDS             1185..3299
                     /locus_tag="PLS88_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOU07132.1"
                     /translation="MDLSKTPEKNLGAPIRNIGILAHIDAGKTSITERILHVAGVREK
                     AGNVDDGTTTTDYLSVERQHGITIKTAAVRFIHKGVLFNLLDTPGHVDFSAEVDRVLR
                     VLDGAVIILCAVSGVQVRTGFIATGCRAHHIPRIYFINKMDRRGADFFGAVKEIAGEL
                     GEPVLPLQIPTFEDYRWNGIVDLVDMCWYPALSNKEENETETKSNEPRIAPAQYPLPI
                     EEAPITEEMRNLALESREKLLDRVSMYDDSLMEKLISGKIVSEKEIRSALSLPIWRQE
                     LTPILCGSSFSDISSRLLLDAIIDYLPDPYSRGCPPAKDMKTGKEIRLEAKPDAPFSA
                     YIFKTALDVNLNTLAWVRLWSGRIHEGMKAMAMPFKKIAQIKHLYGINGAEIEQIDSA
                     AAGEIVGAYLSYMEAGSTLCEPHLQLVYESFKNPEPLVSIALEPNSQQDLPKLRNALK
                     RFSLEDAGLMIDEEKETGRITIAGQGELHLDIVLERLQKEYGLHVRAGNPQVPKVERL
                     IKPVHIEEEFTGDFAGEKLSATLGISLENQKGKDENQVIFAQGLRLSANYEAALHRAA
                     ETAFAVGPSGGWPIVGAKLIVERIIAPMSGDRRAETAVEVAASAILRKAVSLAGSNIL
                     VPIVQVSVEVPDTWFGAALASLQARGARIEAVEDEGGIKSIIAYAPMENLFGYTTSLR
                     SITEGRGIYQAKFDHYGNLNEY"
     gene            3538..4728
                     /gene="tuf"
                     /locus_tag="PLS88_07030"
     CDS             3538..4728
                     /gene="tuf"
                     /locus_tag="PLS88_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOU07133.1"
                     /translation="MAKEKFERTKPHMNVGTIGHIDHGKTTLSAAITMYCGRKFGDKV
                     LKYEEIDKAPEEKARGITISTRHLEYQSNKRHYAHIDCPGHADYIKNMITGAAQMDGA
                     ILVVSAPDSVMPQTREHVLLARQVGVPAIIVFLNKIDLVDDPELIDIVEEEIRDLLNF
                     YGFPGDKTPIIRGSAFKAMTEPDNPEATKCIEELLDAMDSYFPDPVRDIDKPFLMPIE
                     DVFTISGRGTVVTGRVDRGIIRVNDTVEIVGIHPTKTTVVTGVEMFNKTLDEGRAGDN
                     IGTLLRGIEKKEVERGQVLAKPGSITPHTKFKGQIYCLTKEEGGRHSPFFTGYRPQFY
                     FRTTDITGMVTLPEGRDMIMPGDNSEIEVELIYPIAMEKGLKFAIREGGHTVASGQVT
                     EIIA"
     gene            5001..5309
                     /gene="rpsJ"
                     /locus_tag="PLS88_07035"
     CDS             5001..5309
                     /gene="rpsJ"
                     /locus_tag="PLS88_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOU07134.1"
                     /translation="MMKDRIRVKLRGFDVRLVDDSAKSIIKTVQDAGSKVSGPVPLPT
                     RINKFTVLRSPHVHKKSREQFEMRTHKRLIDIIEPTPEVMDALMKLELPSGIDVEIRQ
                     "
     gene            5389..6012
                     /gene="rplC"
                     /locus_tag="PLS88_07040"
     CDS             5389..6012
                     /gene="rplC"
                     /locus_tag="PLS88_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOU07135.1"
                     /translation="MIGVIGKKIGMTQVFDEIGQVVPVTVVQVAPNLVVGKKTAEKDG
                     YNAIVVGVYEKKKTRVTKPYAGQFPESIAPTRILREFRNFEGEVEIGQSIDASILSGV
                     RYVDVIARSKGKGFQGVVKRWGFEGGRGSHGSKFHREPGSTGQSTFPHHSFKNIKMPG
                     HMGNERVTMQNLRVVRVDPEKGVVLIRGAVPGPRNCDVLIRKSIKKS"
     gene            6027..6674
                     /gene="rplD"
                     /locus_tag="PLS88_07045"
     CDS             6027..6674
                     /gene="rplD"
                     /locus_tag="PLS88_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOU07136.1"
                     /translation="MEAKVISLSGKEPRTIELSDAVFGLPINEDVIWYAVTNELANAR
                     VGTASTRDRSEVRGTNRKPFSQKGGGRARHGDLKANIFVGGGVSFGPKPRDYSYSIPR
                     KEKQLSLKTILSLKIQDELMTIIEDFTVESGKTKDLIKVLSNVVDIKNPERTVLILKD
                     DDPMIKRAARNIPWLTYLTYNRLRAHDLFYARRVIMMEGASAKLNEFYAKAAKES"
     gene            6677..6961
                     /gene="rplW"
                     /locus_tag="PLS88_07050"
     CDS             6677..6961
                     /gene="rplW"
                     /locus_tag="PLS88_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOU07137.1"
                     /translation="MEYDAVLIEPVLSEKSNIVRETGQYIFKVDKRATKPMIMDAVAK
                     TFNVHPVSCKIINVTSKPKRLRGRPGQTAEWKKAIVRLKKGETIRVFEGA"
     gene            6982..7812
                     /gene="rplB"
                     /locus_tag="PLS88_07055"
     CDS             6982..7812
                     /gene="rplB"
                     /locus_tag="PLS88_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOU07138.1"
                     /translation="MAIRTFRPVTPGLRHRVQVINTEISSNKSKPAKSLTKGKSSTGG
                     RASNGRISVRHHGGGHKRKYRSIDFKRDKFGVPGKVQSIEYDPNRSANIALIAYVDGE
                     KRYILSPKGLRVGQTVLSGPEAAPEVGNALPLEKIPVGFTVHNVELTLGRGGQLARSA
                     GVSALIAAKEGDYVVLKLPSGEQRMVFKKCIATVGQVGNDEHMNERIGKAGRTRWLGI
                     RPTVRGTVMNPVDHPHGGGEGRGKGYKQPVSPWGQPAKGYKTRDAHKPSSRFIVKRRK
                     "
     gene            7830..8111
                     /gene="rpsS"
                     /locus_tag="PLS88_07060"
     CDS             7830..8111
                     /gene="rpsS"
                     /locus_tag="PLS88_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOU07139.1"
                     /translation="MSRSVKKGPFIEKSLYKKVIEMNRAGEKKLIKTYSRTSTIIPEM
                     VGQTISVYNGKTWVPVYVTENLVGHKLGEFAVTRIFRGHAGSDKKAEKK"
     gene            8122..8490
                     /gene="rplV"
                     /locus_tag="PLS88_07065"
     CDS             8122..8490
                     /gene="rplV"
                     /locus_tag="PLS88_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOU07140.1"
                     /translation="MANNETGYKASAKWLIASPFKVRPVADLVRAKPYPEAIAILDNM
                     PNKGAELIRKVIASAAANALNKNRKLGEDMLYIKELRVDEGPRLKRVWFRARGRADMQ
                     LRRMCHISCIVDEIGKKTEA"
     gene            8492..9340
                     /gene="rpsC"
                     /locus_tag="PLS88_07070"
     CDS             8492..9340
                     /gene="rpsC"
                     /locus_tag="PLS88_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOU07141.1"
                     /translation="MGQKVNPIGLRIGINKEWSSHWYVEPREYAKTLHEDLAIRRRLL
                     ELEETKGADISEIEIIRHPQRITLVIHSARPGVLIGTKGANIDKIGAQIQKVTQKKLQ
                     IKIKEIKKVDTNAQLVAQNIARQLENRQSFRRAMKSAIQNALKGGAQGCKVRLSGRLG
                     GSDMSRTEELKDGRIPLHTLRADIDYGFAEANTTFGKIGVKVWIYQGMVYSQDKNEDA
                     GQLARKARRESLGEPRGERRESGQDRQGRRERVERAELGEQSDRNERRTAPRPEASRA
                     EGEARS"
     gene            9344..9760
                     /gene="rplP"
                     /locus_tag="PLS88_07075"
     CDS             9344..9760
                     /gene="rplP"
                     /locus_tag="PLS88_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOU07142.1"
                     /translation="MLLPKRVKYRKQQRGRIRGEAHSGNTIVFGDYGLVSLEPHWITN
                     RQIEAARIALNRYIKRGGKMWIRIFPDKPYSKKPAETRMGRGKPGPEYWVAVVKPGTV
                     LFELSGVEPKVAEEAMRLAGSKLPVKTKFAIRSEME"
BASE COUNT         2761 a         2201 c         2577 g         2146 t
ORIGIN      
        1 gaaagggagt ctagagctta tatgccgacg attaaccagc taatccgaaa tggaaggaag
       61 caaactcagt ggaagactaa atcgccagcg ctgatgggat gcccacaacg ccgaggtgtt
      121 tgtacacgtg ttatgacagt aacgcccaag aagccaaact cagctctacg aaaggtagca
      181 cgtgtgcgac tcaccaacgg tattgaggtg accgcataca ttccgggaat tgggcacaat
      241 cttcaggagc actctgcggt gctcgtccgt ggcggtcgtg tcaaggacct tcctggagtt
      301 cgctatcata tcatccgtgg aacaaaagat gcgctcggag tcgaagaccg caaacagggt
      361 cgctcaaaat atggcgcaaa gaagccaaag gcttgagcga ggtaataaat gggaagaaaa
      421 aagaaagcaa tagacagggg acacgcccct gatgtcaaat ataattcggt tgtcatctct
      481 cgttttatat gtagaatgat gtgggaaggc aagaaatctg tctgcaccgg cataatgtat
      541 gatgccctag atctccttca gcagagatcc ggtcagcccg ctctagatgc ctttaccaaa
      601 gcaatcgaaa atgtaaaacc ctctattgag gttaaatctc gccgcgttgg cggtgctacc
      661 tatcaggtac cgatcgagat acgtgaatcg cgccgagaag ctcttgcaat gcgatggatc
      721 atccaggcag cccgcgctag aacaggtaag tcaatgcccg aatgtcttgc cgatgagttt
      781 ttcgatgcct ttaacaacac tggagccgca atcaaaaaga aagacgacat gcacaaaatg
      841 gctgaagcca ataaggcatt tgcgcactac cggtggtagt gcacaaatgc tggctatagg
      901 cagcgcgtag cgatgccgtt aagccaataa ggcatttgcg cactaccggt ggtagtgcac
      961 aaatgctggc tatagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcagc
     1081 gcgtagcgat gccgtttagc gaacaaagct tttgcgcatc attcaaagcc gccttccaaa
     1141 ggcggctttt ttctttgggg tggctctgat atattgatat tgcgatggat ttatcaaaga
     1201 cgcctgaaaa aaacctcgga gcgcctatta gaaacatagg tattctcgcc catatagatg
     1261 ccggcaagac atcgattacc gaacgtattt tacatgtcgc gggcgttcga gaaaaagcag
     1321 gaaatgtaga cgacggcaca acaactaccg actacctaag cgttgaacgc caacacggaa
     1381 taaccataaa aacagcggcc gtacgcttta ttcacaaagg agtcctcttc aatttattgg
     1441 ataccccagg tcatgtagat tttagtgctg aggtagatag ggttctacgt gttctcgatg
     1501 gtgcggttat catcctctgt gcagtatctg gcgttcaggt tagaactggt tttatagcta
     1561 ccggctgcag agctcatcat attccgcgaa tctatttcat caacaagatg gacagacgag
     1621 gcgccgattt ttttggcgca gttaaagaga tagcaggcga acttggcgaa ccagttctac
     1681 ccctccagat acctactttt gaagattatc gctggaatgg gattgtggat ctagtcgaca
     1741 tgtgctggta tcctgccttg tcgaataaag aagaaaacga gacagagact aagagcaatg
     1801 aacctcggat agcaccagcc caatatcctc ttcctataga agaagctcca attaccgaag
     1861 agatgcgcaa tctagctctt gaatctcgag aaaagcttct cgatcgcgtc agcatgtatg
     1921 atgattcctt gatggaaaaa ctgattagcg gaaaaatcgt ctctgagaag gaaatccgat
     1981 ccgcgcttag tctaccaatc tggcggcagg aactcacacc gattttatgt ggttcttctt
     2041 tttcagacat ttccagtcgg cttcttctgg atgcaataat cgactatctt cctgatccat
     2101 attctcgcgg ctgccctcct gcaaaggaca tgaaaacagg caaggaaatc agactggaag
     2161 ctaaacccga tgctccattt tccgcctata ttttcaaaac agcccttgat gtcaatctca
     2221 ataccctggc atgggttcgc ctatggtcag gccggatcca cgaaggcatg aaagccatgg
     2281 caatgccttt caaaaagatt gctcagataa aacatctcta tgggataaat ggggccgaaa
     2341 tagagcaaat cgattcggca gccgcaggag aaatagtagg cgcatatctt tcctatatgg
     2401 aagcaggcag cacactttgc gagcctcatc ttcagcttgt ctatgaatct ttcaaaaatc
     2461 ccgaaccctt ggtgtctata gcgctggaac caaactccca gcaagatctc ccaaaactgc
     2521 gcaatgccct taagcgattc agccttgaag atgcaggcct aatgatagat gaagaaaaag
     2581 agaccggtag aataacaata gcaggtcaag gagaattaca tctagacatt gtgctcgagc
     2641 gcttacagaa agaatatgga ctccatgtac gggcagggaa tcctcaggtt cccaaggtcg
     2701 agcgactcat aaaacccgtc catatcgaag aagaattcac aggggatttt gctggagaaa
     2761 aactttcggc gacccttggc atttcattgg aaaaccagaa aggcaaggac gaaaaccagg
     2821 tcattttcgc acagggattg cgcctttcgg ctaattacga ggcagctctg catcgagcgg
     2881 cagagacagc ttttgccgtt ggaccatcgg gtggatggcc gattgtaggt gctaaactga
     2941 ttgttgagcg gattatagcc cccatgagcg gcgatagacg agcagagacc gcggtggaag
     3001 ttgcggcatc tgcgattctg cgtaaggcag tgagtcttgc tggcagcaat atacttgtgc
     3061 caatcgttca ggtttctgtt gaagtaccag atacatggtt tggcgctgcc ttggcttcgt
     3121 tgcaggctcg gggagctcga atcgaagccg tagaggatga gggaggcatc aagtccatca
     3181 tagcttacgc gcctatggag aacctctttg gatacaccac ttcattgcga tctatcacgg
     3241 aagggcgtgg aatttaccaa gcaaaattcg atcattatgg caatttgaat gaatattgag
     3301 atggattttt ataatttcct gacatatagg cacatatatt ttccaggaaa gaaaaaagag
     3361 cttttcttct ataaggctat tgcgcaatct caattctttg cttataatcc gaacgctcaa
     3421 acaagtaaat tatggcgtag tatccggcgg catcgatgcc ggcggacggt attcgtggta
     3481 cttttatgta ttgaaatatt gcgaatagcc ggtgacgaag ccaaggagga gatttatatg
     3541 gccaaagaaa aattcgagcg taccaaacct catatgaacg ttggtaccat cggacacatc
     3601 gaccatggca agacgacgct tagtgcggcc atcaccatgt actgcgggcg caagttcggc
     3661 gacaaagttc taaaatacga agaaatcgat aaggcaccgg aagaaaaggc acgcggtatt
     3721 accatcagca ccagacacct cgagtatcag tcgaataagc ggcattatgc acatatcgac
     3781 tgcccgggtc acgcagacta catcaagaac atgatcactg gtgcagcgca gatggatggt
     3841 gcaatcctcg tcgtatccgc tcctgactct gtcatgccgc agaccagaga gcacgtgctg
     3901 ctggctcgtc aggtgggagt accagcgatt atcgtatttc tcaataagat cgatctcgtc
     3961 gatgaccccg aactcatcga tatcgtagag gaagaaatac gggacttgct gaacttctat
     4021 ggattccccg gcgataagac acccattatt cgcggttctg cctttaaggc aatgaccgag
     4081 ccggacaacc ccgaagccac taagtgcatc gaagagcttt tggatgcgat ggacagctac
     4141 ttcccagatc cagtgcgcga catcgataaa cccttcctta tgcctatcga ggacgtcttt
     4201 acgatctccg gccgcggcac tgtcgttacc ggtcgtgtgg atcgcggtat aatccgtgta
     4261 aatgatactg tagagatcgt gggtattcat ccaacaaaga caactgtcgt tacgggtgtc
     4321 gagatgttca acaaaacgct ggatgaaggt agggctggcg acaatatcgg tactcttcta
     4381 cgtggaattg aaaagaaaga agtggaacgc ggacaagttc tcgccaaacc ggggagcata
     4441 actccgcaca caaaattcaa gggtcagata tattgcctta ccaaagagga aggcgggcgc
     4501 catagcccct tctttacagg ctatcgtccg cagttctatt tcagaaccac cgatataacc
     4561 ggtatggtaa cactgccaga aggccgagac atgataatgc ccggcgacaa tagcgaaatc
     4621 gaagtagaac tcatctaccc gattgccatg gagaaagggc ttaagttcgc tatccgcgaa
     4681 ggtggccaca ctgttgcctc aggtcaagta accgagatta ttgcataaga gtttcagggc
     4741 tgattgctta aggtttgctt tatgagccct aagggaacaa cacgattggc ttatgcaaag
     4801 gcaataaatt agaggttagc tactggacaa atatttttat gagtgctagt atgccgtgcc
     4861 cttcgttgag aggggcacgt ttttgtgtaa tttggtagca cgataccaag ggtggattcc
     4921 ccaatctgcc cgcacctatc gtcgtaggcg cgcctgcctg ggtacggcct gcggtgcaag
     4981 tgcaagagat ctttggagga atgatgaaag acagaatccg agtcaagctc agaggctttg
     5041 acgtgcgcct agtggatgat agcgccaaaa gcataatcaa aactgtgcaa gatgcgggct
     5101 cgaaggtttc cgggccagtg ccgcttccta cgcgcataaa taaatttacg gtgctgcgct
     5161 cgcctcatgt gcacaagaag agccgtgagc agttcgagat gaggacacat aagcgcctaa
     5221 tcgacataat cgagccgacg ccggaagtta tggatgcgct catgaaactc gaacttccct
     5281 ctggaatcga tgtcgaaatc aggcaataga cttttgtccg tcgcggcaaa taagagaatg
     5341 ctgtaacgcc cggcttagtg ctggaagcgt tcaacgcagg agtaccgaat gatcggtgtc
     5401 attggaaaga aaatagggat gacccaagta ttcgacgaga taggccaggt tgtgcccgta
     5461 accgtcgttc aggtcgcacc taatcttgta gttggaaaga agacagccga aaaagatggc
     5521 tacaatgcca ttgtggtagg tgtatacgag aagaaaaaga ctcgcgtaac caaaccctat
     5581 gcaggccaat tccccgagag cattgcgccc acaagaattc tccgagagtt tagaaatttt
     5641 gaaggagaag tcgagattgg ccaatctatc gatgcctcga ttctaagcgg ggtgagatat
     5701 gtcgatgtga tagctcgttc gaaaggcaag ggttttcagg gtgttgtaaa acgctggggg
     5761 ttcgaaggcg gacgaggatc tcatggttcc aagttccatc gagaaccagg ttcgactggc
     5821 cagagcacat ttccgcatca tagcttcaag aatattaaga tgcccgggca tatgggcaat
     5881 gagcgagtta ctatgcagaa cctgagggta gtaagagttg atccagaaaa aggcgttgta
     5941 ctcattcgcg gtgctgtccc aggtccacgg aattgcgatg ttcttattcg caaatcgata
     6001 aagaagagct gatggcggag gactgtatgg aagctaaagt tatttcccta agtgggaaag
     6061 agcccagaac aatcgagctg tccgatgccg tcttcgggtt gcctatcaat gaagatgtca
     6121 tttggtatgc agttactaac gagctggcaa atgcccgagt tggtactgcc tcgacaaggg
     6181 atcgaagcga agttcgtggc actaaccgta aacccttctc gcagaagggt ggcggtcgcg
     6241 ctcgccatgg cgatctgaag gcaaatattt ttgtgggggg cggcgtatcc ttcggaccaa
     6301 agcccagaga ttactcctat tcgattcctc gcaaagaaaa gcagctttcg ctcaagacta
     6361 ttctgagttt aaaaatccag gatgagctta tgacgatcat cgaggatttc acggtagaat
     6421 cgggcaagac aaaagacctc atcaaagtgc tatcaaatgt agtcgatatc aagaatcccg
     6481 agaggacggt cctcattcta aaggatgacg atccaatgat aaaacgcgcc gcaaggaaca
     6541 ttccctggtt gacatatctt acatacaacc gattgagggc tcatgatcta ttctatgcac
     6601 gaagggtcat aatgatggaa ggagcttccg caaagctcaa cgagttttac gcgaaagctg
     6661 ctaaggagag ctgattatgg aatacgatgc agttcttatt gagccggtgc tctccgaaaa
     6721 gagcaacata gtgcgcgaaa ctggtcagta tatctttaag gtcgataagc gagccacaaa
     6781 gcccatgata atggatgccg ttgcaaagac attcaatgtg catccggtga gctgcaagat
     6841 catcaatgta acctcgaagc caaagcggct acgcggcaga ccagggcaga cggcagaatg
     6901 gaaaaaggcg attgtcagac tcaagaaggg tgagacgatc agagtgttcg aaggcgcatg
     6961 agaagaatat aaggatagaa tatggcgatc agaacattta gacctgtcac gcctggactt
     7021 cgacaccgtg tgcaggtaat aaacacagag atctcgagca acaaaagcaa gccagccaaa
     7081 tccctcacaa aaggaaagag cagcacgggt ggcagagcat caaatggccg catttccgtc
     7141 aggcatcatg gaggcggtca taagcgcaag tatcgaagta tcgatttcaa gcgcgataaa
     7201 tttggcgttc ctggcaaggt gcagagtata gaatacgacc cgaatcgaag tgcgaatatc
     7261 gcgctaattg cttatgtaga cggcgagaag cgctatattc tcagccccaa gggtcttcgg
     7321 gttggacaga ctgtgttgtc gggtccagaa gccgcgcccg aggtcggcaa cgcgttgccc
     7381 ctcgaaaaga ttcccgtcgg cttcacggtg cacaatgtcg agctcaccct cgggcgcggc
     7441 ggtcaactcg cccgctccgc aggcgtcagc gcgctcatag ctgccaagga aggcgattat
     7501 gtggtgctca agctgccttc tggcgagcag cggatggtat tcaagaaatg cattgcgacc
     7561 gtaggccagg ttggcaatga cgagcatatg aacgagcgga taggaaaagc agggcgtacg
     7621 cgctggcttg gcataaggcc tacggtacgc ggtacggtta tgaaccctgt agatcatccg
     7681 catggtggtg gagaaggacg aggcaaggga tacaagcagc ctgtttctcc atggggtcag
     7741 ccggcgaagg gctataagac ccgcgatgcg cataagcctt cgagccgctt tattgtcaag
     7801 cggcgcaagt gagatagagg agtaaactcg tgtccaggtc ggtaaaaaaa gggcccttca
     7861 tcgagaagag cctgtacaaa aaggtaatcg agatgaacag ggcgggagag aaaaagttga
     7921 taaagacata ctcccgcaca tcgacgatca ttcccgaaat ggtcggtcag acgatttcag
     7981 tctataacgg caaaacttgg gttccggtct atgtgaccga aaaccttgta ggccataagc
     8041 ttggcgaatt tgccgtgacc cgcatattcc gtggtcacgc aggcagcgac aagaaagccg
     8101 agaagaaata ggggtgcgaa aatggcgaat aatgaaactg gctataaagc ctcggcgaaa
     8161 tggctgatcg cctcgccgtt caaggtgcgc ccggtcgcgg atcttgttcg agcaaagcct
     8221 tatccagagg caattgccat attggataat atgccgaaca agggagccga attgattcgc
     8281 aaggtaattg cttctgcggc cgctaacgcg ctcaacaaga accgcaagct gggagaagac
     8341 atgctctata tcaaagaact gagggtggat gaaggccccc ggctcaagag agtttggttc
     8401 cgtgcacgcg gaagagcgga tatgcagcta aggcgcatgt gccacatctc atgcatagtc
     8461 gacgagatcg gcaagaagac ggaggcgtaa agtgggtcaa aaagtcaatc caattggatt
     8521 aaggattggt atcaataaag aatggagctc gcactggtat gtagaaccgc gcgagtatgc
     8581 gaagaccctt catgaagacc tcgctatccg tcgtcggctt ctcgaactgg aggagaccaa
     8641 gggtgcggat atatctgaaa ttgaaatcat ccggcatccg cagcgtatca ccttagtgat
     8701 ccatagcgct cgtccaggtg ttctaattgg aaccaaaggg gcgaatatcg acaagatagg
     8761 agcccagatc cagaaggtca cacagaagaa actgcagatc aagataaaag agatcaagaa
     8821 agtagatacc aatgctcaac ttgtggctca aaatatcgcg cgtcagttgg agaacagaca
     8881 atcattccgg cgtgctatga aatccgcaat ccagaatgca ttaaagggtg gtgcacaggg
     8941 ctgcaaggta aggctatcgg gtcgtctggg cggttctgac atgtcccgta ccgaagagct
     9001 gaaggatggg cgaattcctc tgcatacact ccgtgcggat atcgattacg gttttgccga
     9061 agcgaatact acttttggta agatcggcgt aaaggtttgg atatatcagg gcatggtata
     9121 ttctcaggat aaaaacgaag atgccggtca gcttgctagg aaagcgcgca gagagtcttt
     9181 gggagagcct cgtggagagc gcagagaaag cggtcaagat aggcaaggac gacgcgagcg
     9241 tgttgagcga gctgaacttg gcgaacaatc cgatcgaaat gaacgccgaa ctgctccgag
     9301 acctgaagcg agccgagcag aaggtgaggc aaggagctaa actatgctgt tacctaagag
     9361 agtaaaatat agaaagcaac agcgcggcag gattcgcggc gaagctcatt cgggcaatac
     9421 cattgtgttt ggcgattatg gccttgtgag cctcgagccg cattggataa cgaatcgcca
     9481 gatcgaagca gcgcgaatag cgctaaaccg ctatatcaag cgtggcggaa agatgtggat
     9541 tcggattttc ccagataagc catattctaa aaaacccgcg gaaactcgca tgggtcgtgg
     9601 aaaaccaggt cctgaatatt gggttgcggt agtaaagcca ggcacggtgc tttttgagct
     9661 atcgggcgtt gaaccaaagg tagccgaaga agctatgcgc cttgctggtt ctaagctccc
     9721 tgtcaagaca aagtttgcga tccgcagcga gatggagtga tcaccatgaa aaactcattc
     9781 aagga
//
LOCUS       DAOSOB010000063         9325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_7328_length_9325_cov_56.559115, whole genome shotgun sequence.
ACCESSION   DAOSOB010000063 DAOSOB010000000
VERSION     DAOSOB010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7328_length_9325_cov_56.559115"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PLS88_07080"
     CDS             complement(<1..856)
                     /locus_tag="PLS88_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07143.1"
                     /translation="MLTHRAMKIILSMLILLYIAQAPNAPSIYAQSTETQEKPKQTPY
                     IEGFEFHLKDVRETAVLRYLDMQIGQKFASQSDLDDFIAEKQRLLQENRVFNEEAAIW
                     YEQRNPSNPEAITIIVRGYATLTALAVPIPKYTEDDGFFLAFRYKDFNFLGMLEPLVF
                     SLDHYFLGGNTTIGAEFGYSPRFMNSQWEFSLTSSVDIGRNLDPAFSDTAISLSTEYP
                     ISEAPEYWAIVPSLSYTYTKSSKIHVGIASLSLIRPIFLLFPGNIELQSSYKQENDAG
                     SWSYLAIAE"
     gene            complement(890..1990)
                     /locus_tag="PLS88_07085"
     CDS             complement(890..1990)
                     /locus_tag="PLS88_07085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU07144.1"
                     /translation="MRVLHIVTRSDSGGAQCMISTLAALQSQSGHIVAVASGLEGAGI
                     AWHSLPASIEKIPVRHLKRNIAPWDDLLALSEIRHICMGWKPDIVHIHTSKAAALTRI
                     AINPNRYPIIYTMHGYDQLKVANKKLLFIDKMLSPRTSAIVAVSHYDLRRMQTDGYHP
                     KLIANGVKAPSGQKSDASELTLALQLLEDLRKKESPTGLMIARPSSPKRPELVRESAR
                     QLKGLCSIVWIGGNARADDPPTFYALGPISDAGRLIPFCDFVLLPSDHEGLPMTLLEC
                     LAAGKPFIASNVGGIPELLKYADLPIAPGFAVENSAEDFASAIHSLADDIELRKAMGA
                     SGKKLWEKCYTAEIMAAEYESLYKHCKQIKKE"
     gene            complement(1990..3147)
                     /locus_tag="PLS88_07090"
     CDS             complement(1990..3147)
                     /locus_tag="PLS88_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU07145.1"
                     /translation="MKTRDSLFATSLRFLPFLFALAFSFSGYTNIGIVVILLLSFWYF
                     ILNWQIVIRDRWALCVIGFFLAIAAKDGIMLALGKGSFEAFAKSGSRVVVLAGCSAML
                     YAHSREDIERALSVGFSVTFFYLFIIFIFMRFKILPMIYNANTFGMVGIWAAILTGTN
                     KWQNKTKNSKLQALAILISMFILVLQDAFFSGSFEGSRTAVFAMIAALCFILLSETKY
                     AIILARLELAVVLVFIFIGSMVFIPQFNDLLANRQYLWQAFLRKGLEQPLLGWGFTTE
                     AANIHLVSSYMIDTPFYDSFAKTGLGPHNSFLAMFFENGVIALAGYITLLIARIRAIR
                     VPLTMFDVSLLAYFIFMSLDAMAPGGLTVLGYFLGLCILAVPEKAQKNKDT"
     gene            complement(3144..5276)
                     /locus_tag="PLS88_07095"
     CDS             complement(3144..5276)
                     /locus_tag="PLS88_07095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU07146.1"
                     /translation="MIEPLITVLTPTWNRASTLPRLKASLEAQTYPNFEWLIVDDGST
                     DGTADLVRQWQLDSALVIILIERQNGGKHRSVNDGVRAAKGRWIYIVDSDDKLPNRAL
                     ENIASHLPSIEAIPNVAGLSGLKADFSGHIIGDCFPTGLEFCDAISLTYRYGIRTDKA
                     DVYKKEILLHFPFPEFKGEKFLTEAVVWYRIANADYVLLLTNEVLYECEYRPDGLSAH
                     SLELRIANPIGTLLFYKDMLGYDLPYRAKLREASNYIRFRKHYKWSPSFDPKNFESVK
                     QVSFPIKVRFPAFIVGNIAYVFDRLALARSKNPQNVCDVSVSKPSDSEKTIHVLHVIN
                     SLSQGGAERFLASLLPRLKEQHIENTVLALNLESAVYAPMLEKAGISIYGTHLRHFVS
                     IENGKKSNKRLQALQIYSVLRVLDVLRAIRYLQPDIVHAHLAPSFHWCALASLFARKP
                     IYITTEHASKNRRMSIPFFKWIEKACYVRYTSIICVSSSVAQALSKWLRIPPSKLQTI
                     ISGIDIEQFSNSATPVAEVVQFLAGRIGIAMTARFVPIKDHPIALEILAKLPESYALI
                     LIGDGPERPHIQDLAAKMQLEQRCLFLGSREDVPDILAACRLYIQTSQQEGLGLAVAE
                     AMAAGLPVVVRNTSGLSDLVDGAGLFFDALDIEKAVAQILSLESIDLREKIVLAEKER
                     VKYLSIERTAHEYGSYYRQILRAKKAIE"
     gene            complement(5273..6529)
                     /locus_tag="PLS88_07100"
     CDS             complement(5273..6529)
                     /locus_tag="PLS88_07100"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU07147.1"
                     /translation="MSSERHVIERNEPHILYVRGERIEQIKGGADHVSNSLLYMLRTY
                     FQHSVNELYLYEFCKRPTSRQAVLFDALQGRFFPYGRQEAAQLRRIIPSYDFVFLDQS
                     VYGRLLSDARISRPDLKTAVLFHNVECEFYAQRVWRAGRIHNALLIPAILRAEKEALR
                     ADLVMTLTRKDSKKLQCLYGRSADCIIMPVVTDRYNRALAEQVKSKNKTDKFNMLFAG
                     SAFPPNIEGIRWFVHNVLPQVPGELFIVGRNFEKYRAQLAADRVHVIGTVDEVDSWYY
                     QSDCVVSPIFWGSGIKVKTAEAFMLGRTIVGTKESFVGYDPIAANAIVVDKEAQFVEA
                     LQRLYSQIMSGSPKENAQARVYYIRELSLEAQYARFSAALDNLFAKANVLSENERQEI
                     KPMRKYELKGKDKHKRKLSEPRRRIM"
     gene            complement(6519..7460)
                     /locus_tag="PLS88_07105"
     CDS             complement(6519..7460)
                     /locus_tag="PLS88_07105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU07148.1"
                     /translation="MSEQSISVIVPTYKRAHLLSLTLPTYLQPETSELIVVDDCSPDK
                     TREVVESLAADDPRIRYIRTDANRKQPHAKNLGISNARGEYLYFGDDDSVLLPGSLER
                     LITTLHDYGALIVGACAPYITRLSKDKDFSSIFEEILGEIEQSERLQKTKINKRLIDP
                     VSLEARFNIRTKEPVEVPFVQASFLVSRELAEKICFDEHYFGNCYREETDFLIRAHNT
                     GAKIIYEPRAIQANLPRSIAIGGAHDGSSNFLIRKLSYFKSALGNNWYFLTKNKEALQ
                     KAFGLNISPLHRQAVFAMIIMRVIVSYPLRKVIGHEL"
     gene            complement(7453..8697)
                     /locus_tag="PLS88_07110"
     CDS             complement(7453..8697)
                     /locus_tag="PLS88_07110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU07149.1"
                     /translation="MGKTSNFAQNIGESKSGHILRSRKIIIISPNGLSDAGGVERVML
                     YAFRALSEHGFQVGIIEQKALAKSKIGQFFSIFKRGVLGFLGQSFGLSIIANRERRSG
                     AFIINNGYAAFFARSDILFCHGCVRAARLTQHRVCLEQHRLDLSKLQEHFDNTEIPRP
                     IRLIESLKLSIYGPDEIAEMIAGNRARIIVAVSEHSQRDWQRYYHLSSGKFEHLPNTV
                     DTKHLQPTSELNEQESSKQSKRVLRILFVGSLTYLKGLDRLRTTVEMARETGKNWEFI
                     IATPSSLNTEHFSSKPNVTIHKVVSFAELPNLYRSCDLLYFPLIYEGFEMVTLEALSS
                     GLPVVGTDVGAISVFCEAEFPGVYRIEPDNFAQVSDTFKIAAAQWKNPAEKHRLHNLI
                     DENYGISIWSQKLIEIVEACYE"
     gene            complement(8700..>9325)
                     /locus_tag="PLS88_07115"
     CDS             complement(8700..>9325)
                     /locus_tag="PLS88_07115"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOU07150.1"
                     /translation="VNAAKEESTRLLQNSFSAIALFVIPAVFGLVAIADDLIQIFAGA
                     RFAPAASSMRITAWIVPIVAFSNALQMQILIPQQKEKSMLISFSIGIVVTGLVLCFAV
                     KPFGQVGAALGMLAGETSVLVAHICFCGRKAISMVFSRKRLFHYLLGGALCGLVAYFI
                     SKLSGIMIVVRLILAIGTSGLFYLAFLLAVKDDFALFILRSGNKNTR"
BASE COUNT         2671 a         2064 c         1984 g         2606 t
ORIGIN      
        1 tttctgcaat agctagatat gaccaagaac cggcatcatt ttcttgttta tatgatgact
       61 gcaattctat gtttccagga aagagaagga aaatcggcct tattaggctc aagctagcaa
      121 tccctacatg gattttgctg ctctttgtat aggtatagga cagcgaagga acaattgccc
      181 agtattcagg tgcctcagat ataggatact cagtcgaaag gctgattgca gtatcgctga
      241 aggctggatc gaggtttcgg ccgatatcaa ctgagctcgt aagcgaaaac tcccattgag
      301 aattcataaa gcgaggcgag tatccaaact cagcaccaat tgtcgtattt ccacctaaga
      361 aataatggtc caaggaaaac actagtggct ccagcatacc gagaaagttg aaatccttat
      421 agcggaatgc aaggaaaaaa ccatcgtctt cagtgtattt aggtattggc acagcaagtg
      481 cggtcaaggt agcatagcca cgcactataa tagttattgc ttctggattg gatggatttc
      541 gctgttcata ccagattgct gcctcctcgt tgaagactct attctcctga agaaggcgct
      601 gcttttctgc aataaaatcg tcgaggtcgg attggcttgc aaatttctgg cctatttgca
      661 tatccaaata tcgaagaact gctgtctctc ggacatcttt caaatgaaac tcgaatccct
      721 caatataagg tgtttgctta ggtttttctt gtgtttcagt gctctgcgca taaatgcttg
      781 gagcattagg agcttgcgct atatatagca aaatcagcat cgaaagtatt attttcatag
      841 cacgatgagt cagcattcta aacgctcctt atgcaatttc ataattcttt tattcctttt
      901 tgatctgctt acagtgctta tagagcgact catattctgc agccatgatt tcagcagtat
      961 agcatttctc ccagagtttt ttgccactcg ctcccattgc tttacgcagt tcgatgtcat
     1021 ccgcaaggct atggatagct gaagcaaaat cctctgcgct attctccaca gcaaatcctg
     1081 gagctattgg taaatccgca tacttaagaa gctcggggat gcctcctaca ttagaggcga
     1141 taaaaggctt acctgcagcc aaacattcga gaagggtcat cggcaaacct tcgtggtcag
     1201 atggcaagag gacaaaatcg caaaaaggaa taagtctgcc ggcatctgaa atcgggccaa
     1261 gcgcataaaa ggtgggtgga tcatcagctc gagcattgcc gcctatccaa acgatactgc
     1321 aaaggccttt tagctggcga gccgactctc gaactagttc gggccgcttg ggagaagagg
     1381 gtcgagctat cattagccct gtcggcgatt ccttttttcg caaatcttct agaagctgta
     1441 gtgctaaagt caattcagaa gcatcggatt tctggccgct tggagccttt acgccattag
     1501 ctatcagctt tggatgatac ccatcggttt gcatgcgcct aagatcgtaa tgagagacag
     1561 caacaatcgc cgatgttctt ggagaaagca ttttatctat gaaaagaagc tttttgttag
     1621 caaccttgag ttggtcgtag ccatgcatcg tatagattat tggatatcta ttaggattta
     1681 tggctattct tgtaagcgcc gcagctttgc ttgtatgaat atggacgata tcgggtttcc
     1741 agcccataca gatatgacga atttcggaga gtgctaatag atcgtcccaa ggtgctatgt
     1801 tgcgctttag gtgccgaact ggtatttttt ctatcgaggc cggaagagaa tgccatgcga
     1861 ttcctgctcc ttccaagcca gaagcaactg caacgatatg gccagattga ctctgcaaag
     1921 ctgcaagggt agagatcatg cattgcgcgc cgcccgagtc agatctggta actatatgca
     1981 gaactctcat caagtatcct tgttcttttg agctttttct ggcactgcaa ggatacatag
     2041 tcctaggaaa tatcctagta ctgttagtcc tcctggagcc atcgcatcaa gagacataaa
     2101 aataaaatag gctaaaagag agacatcaaa cattgtgaga ggcactcgta ttgcccgaat
     2161 ccgagcaatg agcaatgtta tgtatcctgc aagagcgata acaccatttt cgaaaaacat
     2221 ggctagaaag ctattgtgag ggccaaggcc tgtcttggca aagctatcat agaacggtgt
     2281 atctatcata taggaactca caagatggat attagccgct tctgtagtaa atccccagcc
     2341 aagcaaaggt tgctcgaggc ctttccttaa gaatgcttgc caaagatatt gtcgatttgc
     2401 taataaatcg ttgaattgag gaatgaatac catcgaaccg ataaaaataa aaacaagaac
     2461 aactgcaagt tccaatctag caagtatgat tgcatatttt gtttcagata aaagtatgaa
     2521 gcaaagtgct gctatcatag caaaaaccgc agtcctagac ccttcaaaac ttcctgagaa
     2581 aaacgcatct tgaaggacca gtatgaacat cgagataaga attgctagag cttgcagctt
     2641 actatttttt gttttatttt gccatttatt cgttcccgta agaattgccg cccatatccc
     2701 taccattcca aatgtatttg cattgtaaat cataggtaat attttgaatc gcataaaaat
     2761 aaaaatgata aacaaataaa aaaaagtaac cgaaaatcca accgaaagag ctctttcgat
     2821 gtcctctctt gaatgagcat atagcattgc actacagcct gccagtacaa caactcgaga
     2881 tcctgactta gcaaatgctt cgaagctacc tttccccaaa gcaagcataa taccatcttt
     2941 ggctgcaatc gccaggaaaa aacctatcac acataaggcc catcgatctc ggataacaat
     3001 ttgccaattc aagataaagt accaaaagct aagtaaaaga atgacaacaa ttccgatatt
     3061 ggtatatccg ctaaagctga atgcaagggc aaaaagaaat ggcaagaagc gtaagcttgt
     3121 cgcaaataga gaatcgcgag tcttcattct attgcctttt ttgcgcgcag aatttgacga
     3181 taatatgatc catattcgtg cgctgtacgt tcgatcgaaa gatattttac tctttctttc
     3241 tcagctagta ctatcttttc tcgcagatct atgctctcaa gggaaagtat ttgtgcaaca
     3301 gccttctcta tatccagagc atcgaaaaag aggccagcac catctactag atcggaaaga
     3361 ccactagtgt tccgcactac tactggaaga ccagctgcca ttgcctccgc aaccgccaag
     3421 ccaagcccct cttgctgcga agtctgtatg tataaacggc atgcagcgag aatatccggg
     3481 acgtcctcgc gcgaaccaag gaataggcag cgctgttcga gttgcatctt tgctgctaaa
     3541 tcttgaatat gtggccgctc tgggccatcg cctatcaata tgagagcata gctctctggc
     3601 aatttggcga gaatctcgag cgcaataggg tgatccttta tcggtacaaa acgcgctgtc
     3661 atagcgatcc caattctccc tgcaagaaac tgtacaactt cagcgacagg tgttgcacta
     3721 ttcgaaaatt gctctatatc gataccgcta ataatggttt ggagcttact aggaggaatc
     3781 ctaagccatt ttgagagcgc ttgcgctact gaagagctca cacagataat cgaagtatat
     3841 cgcacgtaac aggctttctc gatccatttg aaaaaaggaa tggacatacg ccgattctta
     3901 cttgcatgtt cggtagttat atagatgggc tttcgagcaa aaaggcttgc tagtgcacac
     3961 caatggaacg aaggagcaag atgtgcgtgg acgatatctg gctgtaggta gcgaattgct
     4021 cgcagcacat ctaagaccct caatactgaa tagatttgaa gtgcttgtag tctcttgttg
     4081 gattttttcc cgttctctat ggaaacaaag tgccgtaagt gagtcccata tattgaaatg
     4141 cctgcctttt caagcatagg agcatatacg gctgactcta ggttgagggc aagaactgta
     4201 ttctcgatat gctgctcttt cagccttgga agaagagaag caagaaagcg ctctgctccg
     4261 ccctgcgaca atgaatttat aacatgtagt acatgaatag tcttttctga atcagatggt
     4321 ttgcttactg atacatcaca gacgttttgg ggattctttg agcgagctag agcaagacga
     4381 tcaaaaacat aagcaatatt tccaactata aatgcaggaa accgtacttt tatagggaaa
     4441 gagacttgct ttactgattc gaagtttttt ggatcgaagc taggagacca cttataatgc
     4501 ttgcgaaaac gtatatagtt tgatgcttct ctcaattttg ctctatatgg caagtcatag
     4561 ccaagcatat ccttgtagaa aagcagagtt cctatcggat tggcaatgcg aagctcgagg
     4621 ctatgtgcag atagaccatc cggccgatat tcgcactcat acaagacttc attcgttaga
     4681 agcaatacat agtctgcatt cgctatacga taccagacca cggcttcggt taggaatttt
     4741 tcgcctttga attctggaaa aggaaaatgt agcaatattt cctttttgta cacgtctgct
     4801 ttatcggttc taattccata gcgatatgtg aggcttattg catcacagaa ctccagcccg
     4861 gtaggaaaac agtcaccgat aatatgtcca ctaaaatctg ccttaagtcc gcttaggcct
     4921 gctacattgg gaatcgcttc tatggatggt aggtggcttg caatgttttc gagtgctcta
     4981 ttgggaagct tgtcatcaga gtccactata tagatccacc tgcccttggc ggcacgtaca
     5041 ccatcgttga cagaacgatg cttgcctcca ttctgtcgct cgatgagaat aatgacaaga
     5101 gcactatcga gctgccactg cctaacaagg tcggcagttc catctgtcga tccatcatcg
     5161 acaatgagcc attcgaaatt gggataggtc tgcgcttcta acgaagcctt gagccgtggt
     5221 agagtcgaag ctcgattcca tgtcggagtg agaacggtta tgagaggctc tatcacataa
     5281 ttctccttcg aggctctgat agcttacgct tatgcttgtc tttgcccttg agttcgtatt
     5341 tgcgcatagg ctttatttct tgcctttcat tttcggacag cacattggct ttggcgaaca
     5401 agttatctaa agcagccgaa aatcttgcgt attgtgcttc gagcgatagt tctcgaatat
     5461 agtagacgcg agcctgtgca ttctctttag gagagccgga cattatttgc gaatataatc
     5521 tctgaagtgc ctcgacgaat tgcgcctctt tatcaactac aatcgcgttt gccgcaatag
     5581 gatcatatcc tacgaaagac tcctttgttc cgacaatggt tcttccgagc ataaaagcct
     5641 cggcggtctt gactttgatg cccgatcccc agaaaattgg cgatactaca cagtctgact
     5701 gatagtacca ggaatccact tcatccacag taccaatgac atgaacacga tccgcagcaa
     5761 gctgagcacg atatttctcg aaatttctac cgactatgaa aagttctcct gggacctgtg
     5821 gcaatacatt gtgcacaaac catcggatac cttcaatatt aggtggaaac gccgatccag
     5881 caaataacat attgaattta tcggtcttat tttttgattt cacctgttct gctaatgctc
     5941 tattgtagcg atctgtgaca acaggcatta ttatgcaatc tgcggaacgc ccatagaggc
     6001 actgaagctt tttgctatct tttcttgtta gtgtcattac aaggtcggct cttaacgctt
     6061 ctttttcggc tcttagaatc gctgggatca gtaaggcatt atgaattcgg cctgcgcgcc
     6121 aaactcgctg cgcatagaat tcgcattcga cattatgaaa taataccgca gttttgaggt
     6181 ctggccgaga gattcgggca tcggaaagaa gccttccata gactgactga tccagaaaga
     6241 caaaatcata gcttggtata attctgcgta attgtgcggc ttcttgcctc ccataaggga
     6301 aaaaccggcc ctgtaatgca tcgaagagga ctgcctggcg acttgttggc cgcttgcaga
     6361 attcatagag atagagctcg tttactgaat gctgaaaata tgtcctgagc atatagagaa
     6421 gcgagttcga tacatggtct gcgcctcctt tgatttgctc tattctttcg cctcgaacat
     6481 ataggatatg aggttcattt ctttcaatga catgcctttc agagctcatg acctataacc
     6541 ttgcgaagcg ggtaagatac aattactcgc ataataatca ttgcaaacac tgcttgccga
     6601 tgtaaaggag aaatattaag gccaaaggcc ttttgtaagg cctctttatt tttagtaaga
     6661 aaataccagt tatttccgag tgcagattta aagtagctca attttctaat caaaaaattg
     6721 ctagaaccat cgtgcgctcc accaatagca atcgagcgtg gaagattggc ctggatagct
     6781 cggggctcgt atatgatttt tgcgcctgtg ttgtgcgctc ggattaaaaa atcggtctct
     6841 tcacgatagc aattgccaaa gtagtgctca tcgaagcaaa tcttttccgc caactcgcga
     6901 gagacgagaa aggaggcttg cacaaagggc acttcaactg gctccttagt tcttatatta
     6961 aaacgagctt cgaggcttac aggatcgatg agccttttat taattttggt tttttgcaat
     7021 cgttctgatt gctcaatctc tccaagaatc tcctcaaaga tagaagaaaa gtctttatcc
     7081 ttggatagcc ttgttatata gggcgcacaa gcgcctacaa tcaaagcacc atagtcgtgc
     7141 aaagttgtta taagtcgttc caaagagcca ggcaaaagaa ctgaatcgtc atccccaaaa
     7201 tatagatatt ccccgcgcgc atttgagata cctaggtttt ttgcatgggg ctgcttgcga
     7261 tttgcatctg ttctgatata gcggattcgc ggatcgtctg ctgcaagact ttcgaccacc
     7321 tcgcgagttt tatctggaga acaatcgtcc accacaataa gctcgctggt ctctggctgt
     7381 aggtatgtgg gaagggtaag agaaagcaag tgagctcgct tatatgtagg cacaatgaca
     7441 gaaatagatt gttcactcat agcatgcttc cacaatctct atcagcttct gtgaccatat
     7501 cgaaattcca taattctcat cgataagatt atgcaatcga tgtttctcgg cagggttctt
     7561 ccattgagcc gcagctattt tgaaagtatc ggagacttga gcaaaattgt cgggttcgat
     7621 gcgatacact ccaggaaact ctgcttcaca aaaaacagaa attgccccta catctgtgcc
     7681 cacaaccggc aatcctgaag agagcgcttc cagcgtgacc atttcgaatc cttcatatat
     7741 caaagggaaa tacaagagat cacaggaacg atagagattg ggtagttcag caaagctaac
     7801 taccttgtgg atagtaacat ttggctttga agaaaaatgt tctgtattta aagatgaagg
     7861 tgtcgcgatt atgaactccc aattcttccc tgtttctcga gccatttcaa ccgttgttct
     7921 tagcctatcg aggcctttga ggtatgtaag tgaccccaca aagagaattc gtagcactct
     7981 tttagattgt ttcgaagatt cttgttcgtt taattccgaa gtaggctgta aatgttttgt
     8041 atctacggta ttgggaaggt gctcaaattt gccagatgaa agatgataat atcgctgcca
     8101 atccctttgt gaatgttcgg ataccgctac gatgatacgt gcacgattcc ctgcgatcat
     8161 ttctgctatt tcatctggtc catatatcga gagcttcaag ctctcgataa gcctaatagg
     8221 cctggggatt tcagtattat cgaaatgctc ttggagcttt gataaatcca acctgtgctg
     8281 ttctaaacac actctgtgct gtgttaagcg tgcagcccga acacagccat ggcaaaacag
     8341 aatatcggat ctggcaaaaa aagcagcata tccattattg attatgaaag caccagatct
     8401 ccgctctcta ttggcaatta tcgataatcc aaaggattgt cctaaaaaac ctaacactcc
     8461 tctcttgaaa atagagaaaa attgaccgat tttgctcttt gccaaggctt tttgttcaat
     8521 tataccaact tgaaaaccat gttccgaaag agcacgaaat gcatagagca tgactcgctc
     8581 tacacctcct gcatcagaga gtccgttggg agagattatt attatctttc gagacctaag
     8641 tatatggccg gatttggatt ccccaatatt ttgcgcaaag ttcgaggtct ttcccatatc
     8701 tatctcgtat tcttatttcc gcttcttaag atgaagagcg caaaatcgtc cttgactgca
     8761 agaagaaatg caagatagaa aagtccactt gtaccaatag ccaatataag ccttacaaca
     8821 atcattatcc ctgataattt gcttataaaa taggcaacta gcccacagag tgctcctcct
     8881 aaaagataat ggaatagtct ctttcgtgaa aacaccatag aaatcgcctt tctcccgcag
     8941 aagcatatat gtgcgacaag gactgatgtc tctcctgcca acatgcctag ggcagcccct
     9001 acctggccaa aaggcttcac tgcaaagcat agcacaaggc cagtcactac gatgccgatg
     9061 ctgaaagaaa ttagcataga cttctctttc tgttgtggta tcaagatttg catctgaagc
     9121 gcattcgaga atgccactat cggtacgatc catgccgtta ttcgcattga agatgctgca
     9181 ggtgcaaatc tagcgccagc aaatatttgt attaaatcat cggcaatagc tacaagccca
     9241 aaaactgcag gaattacaaa taatgcaatg gccgagaaag aattctgcaa aagcctcgta
     9301 ctctcttctt tagccgcatt gacaa
//
LOCUS       DAOSOB010000064         9056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_7646_length_9056_cov_55.123653, whole genome shotgun sequence.
ACCESSION   DAOSOB010000064 DAOSOB010000000
VERSION     DAOSOB010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7646_length_9056_cov_55.123653"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            576..1517
                     /gene="cas6"
                     /locus_tag="PLS88_07120"
     CDS             576..1517
                     /gene="cas6"
                     /locus_tag="PLS88_07120"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HOU07151.1"
                     /translation="MKIIYGKVSFLLDFEKPISWNTLPTFILRSLLGTQLKRICCVQR
                     QLSCEKCNISQSCAYAVLFESPIPKDTKVLPGRNRGAHPFVLTHGKVINSGKQYEFTI
                     TLIGFALQFFPFVVLAFQRAGDEGIFSDQIMFSIAQITDSTNGKSLGVPILNPLSTKI
                     FSFEETDCSIANKANIHFITPARIKHRGQYTLDFDAFSLLQSLYRRIEVLAYFYTDDS
                     SDAQGYRPPADIIITKRNLTWKDFSRYSHRQSAAMELGGALGWIEIQGPIGAREKSLF
                     DAGTIFHVGKNTGFGLGEMSVEWPSISIEEVHDASIE"
     gene            1501..3900
                     /gene="cas10"
                     /locus_tag="PLS88_07125"
     CDS             1501..3900
                     /gene="cas10"
                     /locus_tag="PLS88_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HOU07152.1"
                     /translation="MQASNERDIFPIAVAALLHDIGKFAQRAGRKDLFKTEMEGQILP
                     QDKSSGQYSHLHALYTYGFLEDCLKKSLGSETYAEKIAHLAAYHHNPSSEEDNFITLG
                     DWAASGLDRIKQKQERIEQKFYETPICSVLSLITLPEREAIHQHYLGLGKLDDDDTQL
                     KEKLTQEDYSVLWDQFLIDWKRLPISTNTFQYLVNLDSILERWTSLIPSATYKTEPDV
                     SLYDHSKVTAAFSVCAYAYHAEKKDYSEVSIFNVADKKWLFVYGDVQGIQKHIFNLKK
                     NDKSSKLLRARSFQIEALCKSAAHSLIVRSGVVSQCEIINGGGNFMLLLPNTEKVRIA
                     VENYEKEINGFLLREYLGTLSLNITLGAEVSLQDLTQENTPELLQTIHASNYKGKQKR
                     FQSVLDTQGHYIDHYYEAARESIGEGNSVCTLCDYRPAQRDRKDEEVICSHCDALISL
                     GTRLPKAHAVVLEILDPAEDNAQKKSASFGFFPVVGQLEGSGIQYCINGYGTGSFMAF
                     HPAPYHVPIAIDKNGEPRVLMFEEIAKKATGTQKLAMFKADVDNLGTIFREGLGAKVS
                     LSKYASLSRQMHYFFSVKLNKWIQEQKDYAESIYTVYSGGDDICVLGPWNVIFDFARD
                     IQAQFASFSFHNPSLTLSAGIALADSNMPIPMLAEEAERQLERAKAQPSKNSLCVFSI
                     PVSWQEFDLLYNNGKRYADHLGRQELPVSLFRKLLDLSNRAWSMLAEGDISSTNALWL
                     SQLKYTIARYREMKSCRVPAQFWDNLLAELTSNHYQMMWKSKISLCYALYSIRSITTK
                     I"
     gene            3913..4386
                     /gene="csm2"
                     /locus_tag="PLS88_07130"
     CDS             3913..4386
                     /gene="csm2"
                     /locus_tag="PLS88_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HOU07153.1"
                     /translation="MTDNNQGKAAWRAGYNQRPVQQAANLPSLPSLLTENGKLNAELF
                     KEELLTQIAKAIQNVSKSQMRKVFDEVKRHKFALEAGQKWEDIYPQVLMQKAKISYLC
                     KRKSEDNKIDESYYNNLKKLIFQLADDCRSAEAYKAYATFLEALYGYYYEIARRK"
     gene            4404..5069
                     /gene="csm3"
                     /locus_tag="PLS88_07135"
     CDS             4404..5069
                     /gene="csm3"
                     /locus_tag="PLS88_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HOU07154.1"
                     /translation="MKIQQIKKITGKIKIETGLHIGASTDALEIGGMDNPIIKDPMTS
                     LPYIPGSSLKGKMRSLMEWKLGKISINGSLCSCGAEDCPICRVFGAGISREKDQDAKR
                     RGPTRLIVRDASLSQEFIDKADLIIEEKSENSINRVTAEANPRPLERVAPGVTFDLEI
                     VYRVLDTGDGGAKDEELFKTTVIESLKLLQNDYLGGGGSRGNGKISFIDLKDEVGTPI
                     SFS"
     gene            5099..6028
                     /locus_tag="PLS88_07140"
     CDS             5099..6028
                     /locus_tag="PLS88_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07155.1"
                     /translation="MNCYRAILKVQGLLKIPIVSDTLWGHIAWGIALEEGEEALEAFL
                     QHYDESPPLVLSHAFPCGYLPRPLLRIAPPDSPNIKKLMKIGYLPKELFAQPIGWQML
                     DNLIKNNDLQHVAMASESRIRNAIDRISSTVEGEKLWSEQGLYWYERSTETSRRRLIN
                     QFDVYCFSSWTKDELERRIEQGLRNGYGGKSSIGYGNVKLLDIKEEKPPLQGRRAMSL
                     GTFIPVKGDKLENLSTKIVVRSGKLGSIFSATMNPFKKPLVFYDEGTTFDRPQKDYVG
                     GLVPNVHPDARIHSCGITLYATFQEEEAANDNL"
     gene            6015..7139
                     /gene="csm5"
                     /locus_tag="PLS88_07145"
     CDS             6015..7139
                     /gene="csm5"
                     /locus_tag="PLS88_07145"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HOU07156.1"
                     /translation="MIIYEISITPITPIHIGTGEDLMPFSYTLLKMSPQGQNYKYVRF
                     NDERLVSFMTTDQIERLEALIAKDDFPHLRQTYNEIACKIILSHQECIFYLAEITNEI
                     LNLWQELEKRPQNSFVIQPTICSLYTKKPYIPGSSIKGAIRTAILDPHAQEFAKTHKQ
                     LKEQDMLSVIECMRDQKYKAQADPLRALKITDAIFPARDSRIVGQLALYNRVKGESEN
                     IAINEEVIKSAAISSLCEGDNAEPITTTTTIALDDRLIKKHPFRITPRSFQEIADRCN
                     EFYETALNKEYELFYRDAPDELYNGYKYIDDAFSNLNQEREFMARLGCHSQIEYLTFS
                     TERAPGKKAFETKTRTLFKYKNMLLPMGWVRCALVSEKEA"
     gene            7150..8235
                     /gene="cas1"
                     /locus_tag="PLS88_07150"
     CDS             7150..8235
                     /gene="cas1"
                     /locus_tag="PLS88_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOU07157.1"
                     /translation="MGTVYILSDHGKLAKQNDALMFVSGSGETRKIFLHRTDRIIIAG
                     NIEITGSALRLIMRNQIDTIFLSANGKFNGKLEFEEGKNVFLRKRQYESLKEPDFCLK
                     IARSIAIGKISNQISFMQRINRKSNRINVESTIEQSKRNLAAVEIAKSIESIRGHEGL
                     GSKLYFSAFRLNINPEWAIFKGRSMNPPRDNVNAVMSFLYTLLMYRVDSFIEMEGLDP
                     YAGYLHTLEYGKRSLTFDLMEEYRASICDTLACALFNLGILTASDFEIVDFSVTSDDA
                     PLHQAEADQSETEQIETSEAIKGILLSKVGAKKVAEKFEEKMESVIFYPPENQRMSNQ
                     KIIANQVHRFRRVLQNEEAEYKPFIIR"
     gene            8241..8516
                     /gene="cas2"
                     /locus_tag="PLS88_07155"
     CDS             8241..8516
                     /gene="cas2"
                     /locus_tag="PLS88_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOU07158.1"
                     /translation="MIWLVSYDIANPKRLNKVAAYCEKFGLRLQKSTFQIDTEKDILE
                     NLLEGLKEIMNRKYDSIVIYPLCEDCRRLSLTDGPNNIINPDEVIFL"
     gene            8513..8800
                     /gene="cas2"
                     /locus_tag="PLS88_07160"
     CDS             8513..8800
                     /gene="cas2"
                     /locus_tag="PLS88_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOU07159.1"
                     /translation="MSHWLLIYDISDTKRLRKVAKIANAYGNRVQRSVFEISCSRETI
                     EMIRKRIGLIIEDPDSVAYIPLCQFDYSGIMRFGKSLFDSDYDIDEKHLFL"
BASE COUNT         2792 a         1942 c         1888 g         2434 t
ORIGIN      
        1 gcgtaaaccg gtatgctttt tcgatcatac tgattcctcc aagctataaa aatctttttc
       61 ccctttccta ttcaagagga atctatagtg agccagataa gtctgtaaag atgatatttt
      121 aaaagccgtt ggtgaaaatt ccaaatgcta ctctttgtaa aagctaacgt ttccgtatct
      181 aaactctttt tcaaggatag attcttatca aaagtctttt ttagatgttt ataaaagcca
      241 aagctgggct ttaaatgaaa aatctaaaaa ttatttgata gaattttgag agggacacta
      301 tatgtcccgt atctcctatt atactaaatc ttataaagct ttatttactt ctgatgcaag
      361 ctagtctatt cttataagga ggtcagatat ataaaagcca attctgtttc ggcgaatggc
      421 ttttgtgata attttttatg caatcaggaa taattaaatg tgttttttta ttatttacat
      481 atgtatattt tgatagcaaa aaataattcg tcttcatgaa gttattgtag tcaataattt
      541 ttaaataatg tctaaagatc aataaggaga acgccgtgaa gattatatat ggcaaggtct
      601 cttttttgct cgatttcgaa aaacctatca gttggaatac tcttcctact tttatcctta
      661 gatcgcttct gggtacacaa ctaaagagaa tctgctgtgt acaacgccaa ctttcatgcg
      721 aaaaatgcaa tatctcacaa tcatgtgctt atgcagtatt gtttgagagc cctattccca
      781 aagacacaaa agtcttacca ggaagaaatc ggggtgctca tccttttgta ttaactcatg
      841 gcaaagtaat aaattctggc aaacaatatg aatttacaat aacactaata ggctttgcat
      901 tgcagttctt cccttttgtt gtgcttgcat tccagagagc aggggatgaa ggcattttct
      961 cagatcaaat aatgttttca attgcacaga ttactgattc gacaaatggt aagagtcttg
     1021 gcgttcctat tttaaatcct ttatctacaa agatattttc ttttgaagaa acagattgct
     1081 ctatagcaaa taaagcaaat atacatttta ttacgcctgc aagaatcaaa catcgagggc
     1141 agtacacatt agattttgat gctttctctc ttcttcaaag tctatatcga cgcatagaag
     1201 tattggccta tttttatact gatgattctt cagatgcaca gggatatcga cctcctgctg
     1261 atattatcat aacgaaacgc aatcttacat ggaaagattt ttctcgctac tcgcaccgac
     1321 aaagcgctgc catggagctt ggaggtgcgc ttggatggat tgaaatccaa ggaccgatag
     1381 gagctcgcga aaagagtctt ttcgatgctg gtactatttt tcatgttggg aaaaatacag
     1441 gctttggcct tggagaaatg agcgttgaat ggccatctat ttcgatcgag gaggtacatg
     1501 atgcaagcat cgaatgaacg cgacatcttt cctattgctg ttgctgcttt gctgcatgat
     1561 atcggcaaat ttgcgcagag ggctggtcgc aaagatcttt tcaaaacgga aatggaaggg
     1621 caaattcttc cacaggataa atcgagcggg caatattccc atctacatgc actttataca
     1681 tatggttttc tggaagactg tttgaaaaaa tctttgggat ctgaaacata tgctgagaag
     1741 attgcccatc ttgcagcata tcatcacaat ccatcatctg aagaagataa tttcataacc
     1801 ttgggagatt gggcagctag tggcctcgat cgtatcaaac aaaaacaaga aagaatcgaa
     1861 caaaaattct atgaaactcc aatctgttca gttctctctc ttattactct tccggagaga
     1921 gaggcaattc atcagcatta tctgggtctt ggaaaactag atgatgatga tactcaactg
     1981 aaagaaaaac taacccagga agactactcg gttctttggg atcagtttct aattgattgg
     2041 aaaagacttc cgatttctac caatactttc cagtatctgg tgaatctcga tagcatcctt
     2101 gaacgatgga cgagcctaat tccttctgca acctataaaa cagagcctga tgtctccctc
     2161 tatgatcatt ctaaagtcac cgctgcattt tctgtgtgtg cttatgcata tcatgctgag
     2221 aaaaaagact attccgaagt gtcgatattc aatgtggctg ataagaaatg gctctttgtc
     2281 tatggtgatg ttcagggtat ccaaaagcat atcttcaatc tgaaaaaaaa cgacaaatct
     2341 tccaaactat tgcgtgcgcg aagttttcaa atcgaagcac tctgcaagag tgcagcacat
     2401 agcctaattg tgcgaagcgg agttgtatct cagtgcgaaa tcataaatgg gggcggaaat
     2461 ttcatgctgc ttctacccaa tacagaaaaa gtgcgaattg ctgtagaaaa ctacgaaaaa
     2521 gaaatcaacg gctttctttt gcgcgaatac cttgggacac tctcgctcaa catcaccctg
     2581 ggcgcggagg tttctctgca agatctcaca caagagaaca ctccggagct acttcagaca
     2641 attcatgcct caaattacaa aggcaagcaa aagcgctttc agtccgttct tgatactcaa
     2701 gggcattata ttgatcatta ttatgaagca gcacgagaat ctatcggcga aggaaattcc
     2761 gtctgcacgc tctgtgacta ccgccctgct cagagagacc ggaaggatga agaagtaatt
     2821 tgtagccatt gcgatgctct catctccctt ggcactcgtc tacctaaggc acatgctgtg
     2881 gtcctcgaaa tccttgaccc tgcagaagac aatgctcaga aaaaaagtgc ttcgtttggg
     2941 tttttccctg tagtaggtca attagaaggc agcggtattc aatactgcat caatggatat
     3001 ggcacaggct catttatggc cttccacccc gcgccctatc atgttcctat agctatagat
     3061 aagaatggtg agccaagagt gctaatgttt gaagaaattg ccaaaaaagc tacaggcacc
     3121 cagaagcttg ccatgttcaa agcagatgtc gataacctcg gcactatctt cagagaaggg
     3181 ttaggcgcaa aagttagcct ttcaaaatat gcatctttga gccgacaaat gcactatttc
     3241 ttctctgtca aacttaataa gtggatacaa gaacaaaagg actatgctga atcaatctat
     3301 accgtctact caggaggcga tgatatctgc gtcctagggc cttggaatgt aatcttcgat
     3361 tttgcccgag atatacaggc tcagtttgca tctttcagct ttcataaccc atcactaact
     3421 ctatcggcag gaatcgctct cgcagactct aatatgccaa tccctatgct cgctgaagaa
     3481 gcagagcgcc agctcgaacg agcaaaggca cagccaagca agaattccct atgtgtgttt
     3541 tctatacctg tttcatggca ggaattcgat cttctgtaca acaatgggaa acggtatgct
     3601 gaccatctag gacgtcaaga attacctgta agccttttta gaaaactgct cgatctcagc
     3661 aatcgcgcat ggtccatgct agcggaaggt gatattagct ctaccaatgc tctctggctt
     3721 tcacaactga aatacaccat tgcccgctat cgcgaaatga aaagctgcag agttcctgcg
     3781 cagttttggg ataatcttct agcagagctc actagtaatc attaccaaat gatgtggaaa
     3841 tctaaaatca gcttgtgtta cgcgctctat agcatacgat caataacaac aaaaatataa
     3901 aggagaaaag ccatgacaga caacaatcaa ggcaaagcag cttggcgcgc aggctacaat
     3961 caacggcctg ttcaacaagc agccaatcta ccgagcttgc catccctttt gacagaaaat
     4021 ggtaagctaa atgcagagct ctttaaagaa gagcttctaa cacagattgc taaagctatc
     4081 caaaatgtct cgaagagtca gatgcgcaaa gttttcgatg aggtcaagcg tcacaagttt
     4141 gcacttgaag cagggcagaa gtgggaagat atctatcctc aagtgctcat gcagaaggca
     4201 aaaatcagct atctttgcaa gagaaagagc gaagacaaca agatagatga atcctattat
     4261 aataatttaa aaaagctcat attccagctt gcagacgatt gtagatcagc tgaagcttat
     4321 aaagcttatg cgacattcct cgaggcgctc tatggttact attatgagat tgcgcgaaga
     4381 aaatagacat aaggagatag gcaatgaaaa tccaacaaat taagaaaatc actggcaaaa
     4441 tcaaaatcga aacagggcta catatcggtg cgtccaccga tgcacttgag atcggcggca
     4501 tggataatcc catcattaag gaccccatga ccagcctccc ctatattcca ggttcttctc
     4561 tgaaaggcaa aatgcgttcc ctcatggaat ggaaacttgg aaaaattagc ataaatggca
     4621 gtctttgtag ctgtggtgca gaagactgtc ctatttgcag agtctttggt gctggtatct
     4681 cgcgcgagaa agatcaagat gccaaaaggc gtggtcctac acgacttatc gttcgcgacg
     4741 catcactttc ccaggaattc attgataaag cagatttaat aattgaagaa aagagcgaaa
     4801 actctataaa cagagtcacg gctgaggcca atcctcgtcc tctcgagcgt gtagctccgg
     4861 gcgtaacctt cgatctcgaa atagtctatc gcgtgcttga tactggcgat ggtggagcta
     4921 aagacgaaga gctcttcaaa acgaccgtta tagaatctct aaagcttttg cagaacgact
     4981 acctgggcgg aggtggtagc cgcggcaatg gcaagataag ctttatcgac ctcaaagatg
     5041 aagttggcac gccgatttcg tttagctgaa atcctaaggc ccttcaagga ggtcaatcat
     5101 gaattgctac cgtgccattc ttaaggtgca gggtcttctc aagatcccca tagtgagcga
     5161 tacgctctgg gggcatatcg cctggggtat tgcgctcgaa gaaggcgaag aagcgctgga
     5221 agccttctta caacattatg acgaaagccc accgctcgtt ctctcgcatg ctttcccttg
     5281 tggttacctg ccgaggccat tgcttcgtat agctcctcct gattcgccta acatcaagaa
     5341 gcttatgaaa ataggctatc tacctaaaga acttttcgca caacccattg gctggcaaat
     5401 gctagataac ctaatcaaga ataacgatct gcagcatgtc gccatggcat cggagtcgag
     5461 aatacgcaac gcaatcgata gaatttcttc cacagttgaa ggcgaaaaac tatggagcga
     5521 acagggactc tattggtatg aacgtagtac cgaaacatcg agacgaagat tgatcaacca
     5581 attcgatgtc tattgttttt cttcttggac aaaagatgag ttggagcgtc gcatcgaaca
     5641 gggccttcga aatggatatg gtggaaagag ctctatcgga tatggcaatg tgaagctgct
     5701 cgacatcaag gaagaaaagc ccccattgca aggaaggaga gctatgtcct taggtacttt
     5761 tatacccgtt aagggcgaca aactagagaa cctatcgaca aagatcgtag tccgcagtgg
     5821 aaagcttggt tctatcttta gcgcaaccat gaatcccttc aaaaaacctc tggttttcta
     5881 tgacgaagga accaccttcg atcgccctca aaaagactat gtcggaggcc ttgtacccaa
     5941 tgtgcatcca gatgctcgaa tacacagttg cggaataacg ctttatgcta ccttccaaga
     6001 agaggaggcc gctaatgata atttatgaga tcagcatcac accaataaca cctatacata
     6061 tcggaactgg cgaggatcta atgccttttt catatactct acttaaaatg agcccacaag
     6121 gacaaaacta taaatatgtc cgattcaacg atgaacggct agttagcttt atgacaacag
     6181 accaaataga aaggcttgaa gctctcatcg caaaggacga ttttcctcat cttcgtcaga
     6241 catacaatga aattgcttgc aagattattc tctcccatca agaatgcatc ttctacttag
     6301 ccgagattac gaacgaaatc ttaaatctat ggcaggagct cgaaaagaga ccgcagaata
     6361 gctttgtaat ccagcctact atctgtagcc tttataccaa gaaaccgtac atccccggct
     6421 cttcgatcaa aggagcgatt cgcactgcta tcctcgatcc acacgcgcaa gagtttgcaa
     6481 aaacgcataa gcagctaaaa gaacaggaca tgctttctgt tatcgagtgc atgcgtgacc
     6541 agaagtataa agcccaagcg gatccattgc gagccttgaa gattacagat gctatctttc
     6601 ccgctcgcga ttcgcgcatc gtgggccagc ttgcccttta caatcgagtc aagggggaat
     6661 ctgaaaacat tgctattaat gaagaagtca tcaagtcagc cgcaatcagc tctctttgcg
     6721 aaggagataa tgcagagcct attactacta ccacaactat cgcgctcgac gatagactta
     6781 ttaaaaagca tccctttagg attactcctc gatcgttcca agaaatagcc gatcgctgca
     6841 acgaattcta cgaaacagca cttaacaaag aatatgaact cttttatcga gatgctccag
     6901 acgagcttta caatggatac aagtacatcg acgatgcatt tagtaatcta aaccaagaga
     6961 gagagtttat ggcccgctta ggatgccata gtcagatcga ataccttacc ttctcgacgg
     7021 aacgcgctcc aggtaaaaaa gcttttgaaa ctaaaacgcg cactctcttc aaatataaaa
     7081 acatgctttt gcccatgggt tgggtgcgtt gcgctctcgt cagtgaaaaa gaggcatgag
     7141 ggctaggcta tgggcacggt ctatatcctc tcggaccatg gcaagctcgc caaacagaat
     7201 gatgcactca tgtttgtttc tggcagcgga gaaacacgta agattttcct tcacagaacc
     7261 gatcgcatca tcatagcggg gaatatagag ataactggaa gtgcgctacg acttataatg
     7321 cgcaaccaaa ttgacacaat ctttttgagc gccaatggaa agttcaatgg caaactagaa
     7381 tttgaggagg gaaagaacgt atttctacgc aagcgccagt atgagtccct caaggaacca
     7441 gatttctgcc ttaaaattgc ccgtagcatt gctataggaa aaatcagcaa ccagatctcc
     7501 ttcatgcagc gaatcaatcg aaaaagcaat cgaataaatg tagagagcac catcgaacaa
     7561 tccaagcgca acctcgcagc tgtagagatt gctaaaagca tcgaatctat ccgtggacac
     7621 gaaggcctgg gttcaaaact ttacttttct gcctttcgcc taaatatcaa tcccgaatgg
     7681 gctatcttca aaggaagatc catgaatccg ccgcgtgaca acgtgaatgc agtcatgagc
     7741 tttctgtata ctttacttat gtatcgggta gatagcttta tcgaaatgga aggcctcgat
     7801 ccttatgctg gctatctcca caccctagaa tatggcaagc gaagcctcac cttcgatctc
     7861 atggaggagt atcgagcttc tatctgcgat accctagcat gtgcactctt caaccttggt
     7921 attcttacag ctagtgattt cgagatcgtt gatttttcgg taacaagcga cgatgctccc
     7981 ttgcatcaag cagaagcgga tcagagtgag acagagcaaa tcgaaacgag cgaagcaatt
     8041 aaaggcattt tattaagcaa ggtgggcgca aaaaaggtcg cggaaaaatt cgaggagaag
     8101 atggagagcg tcatcttcta tcctcctgaa aaccagcgca tgtctaacca aaagatcata
     8161 gcaaaccaag tgcatcggtt ccgaagagtc ttacagaatg aagaagcaga atataagcct
     8221 tttatcataa ggtgaacacc atgatatggc ttgtatctta tgacatagcg aaccctaagc
     8281 gccttaataa agttgcagcc tactgcgaaa aatttggtct acggcttcaa aagtccacat
     8341 ttcagatcga tactgaaaag gatatattag agaatctctt agaaggtctc aaggaaatca
     8401 tgaaccgcaa gtacgattca attgtgatct atccactctg tgaagattgt cgccgactat
     8461 ctttgacaga tggacctaac aatatcatca atcccgatga ggttatattt ctatgagcca
     8521 ttggcttttg atctacgata tcagcgatac aaaacggctg cggaaagtag caaagatagc
     8581 taatgcgtat ggtaatcgcg ttcagcgctc ggttttcgag atttcatgct ctagagaaac
     8641 tatcgagatg atccgcaagc gcatcgggct tatcattgag gatcctgatt ctgttgctta
     8701 tattcccttg tgccaattcg attattcagg tataatgagg tttggtaaga gtctttttga
     8761 ttcggattac gatatcgatg agaagcattt atttctgtaa tgaaataatt tttatgaacg
     8821 agcaaacctc ctgttttttc cagaaatgca tttttggcta taggttggca caaaaattta
     8881 tttcccaaaa tagtaacatc ataaacgata tcgcattacg caattcaaat cgatttttcc
     8941 taatgtgcca ccgaatagag caaggtcagc gtatcatatc aactaattca tggtgccata
     9001 aaggaatggt gagcttgcgg ttttgagatt tagacctgat agaagggatt aagact
//
LOCUS       DAOSOB010000065         8879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_7842_length_8879_cov_76.243200, whole genome shotgun sequence.
ACCESSION   DAOSOB010000065 DAOSOB010000000
VERSION     DAOSOB010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7842_length_8879_cov_76.243200"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1052)
                     /locus_tag="PLS88_07165"
     CDS             complement(225..1052)
                     /locus_tag="PLS88_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU07160.1"
                     /translation="MSKHLKSRYPFTMLFLFISSALMIFPFVWMMLSSFKTPADVYSY
                     PPKWIPSSFKFTNYEEVFKKIPFIRFYANSIFVSVVQTVLQIAISAMGAFAFAKLEFP
                     LKNKLFAFMQSSLFVPEIVTIIPLFLLAASAGLIDTYTGLIIPGLNCAFTTLLIMSFF
                     QTIPNELIDAAKVDGCGYIRVFLEIMLPNSKTAIGTASLFAFLTHWRSYMWPLIITND
                     VKMRTLPIGLKYLVTETGTEYQVLMAASVMAIVPVLIVYMLMEKEFVQSITLTGLKT"
     gene            complement(1068..1982)
                     /locus_tag="PLS88_07170"
     CDS             complement(1068..1982)
                     /locus_tag="PLS88_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU07161.1"
                     /translation="MSVKKKKGAQVYSIKKEQHRAAAIFVTPLLITLILLFLLPVILV
                     IIMSFTNWTMTSTARSYVGFRNFVFVLKDPRFWKAFKNTAYYTALKLVLDTGLALFIA
                     VLLDRKIHFRRFFRIAHFAPVVVPITASSLIWLWFYDPGIGPLNQILNFLGLPSMQWL
                     YSEKTAMLSVILFSIWKGIGYNIILFLAGLQSIPESYLEAARIDGASEWQVFFKVKLP
                     LLSPVTSFIIMMGIINCFKVFTEVDVMTPNHGPLESTLLMVSYIYDQSFTRGKMGRGA
                     ASSLILFLIIFAFTMIQRRLGRKEISFD"
     gene            complement(1973..2977)
                     /locus_tag="PLS88_07175"
     CDS             complement(1973..2977)
                     /locus_tag="PLS88_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07162.1"
                     /translation="MKIGVVIGSASGKGFAPEAIISLVPSLQSYDVAVCAGPFGCDLL
                     PSSWSRLKPELSASYVENLKTSVASLLDWGSDMLICIGGDGLGSYVLDAMISHGNKVR
                     LLGIGAGTINVGPIVAFGIEDIPMLDISKLFVKTIDAVEISVDGKHLAYALNDAIIGN
                     SFLGTLEGRVVNLSAKALLDDGRKQEIEPSSDIVSRDFCIRKNGLRIENYMQKPAQII
                     ASPLRKREFFARAIAGALCNASYMDGAAALGLFDTVIVRAATPLRGFENMALSEHLLF
                     GPKDFVEIEGLSRDGDIVVDGNPFHREGEVVSFKLRIDVAEVLVPRDHNSEPEGVLCR
                     "
     gene            complement(3053..4102)
                     /locus_tag="PLS88_07180"
     CDS             complement(3053..4102)
                     /locus_tag="PLS88_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOU07163.1"
                     /translation="MKALVYTGIKQLEVKDVPEPSSDFIVKVLGCGICGTDLKTYQKG
                     HHLFSPPAILGHEFYGLVEKAPIGCGYFAGDKVVVAPYAECGFCDVCIRGAGSLCANK
                     HYVEGGAFCEKVGIASDYIEQGVFHIKELDDVYTLVEPLACVLNGFEQLDLKPTSRAL
                     VVGAGPMGALFALLFRAKGIPVAVVEPSAQRRERIASWGIEAFEPGGAGHVESPRAGQ
                     VESARPGQVESARPGRVEKGFYDIAIIAVNKKELVSEYIKLVADAGTVLMFSGLSSDE
                     IVAIDAYSIHYRQVSLKGSFGYALRHFKEALSLIEAHKEMFSKIITHNYPLELGKEAF
                     EMLASGEALKIVLRP"
     gene            complement(4099..>4793)
                     /locus_tag="PLS88_07185"
     CDS             complement(4099..>4793)
                     /locus_tag="PLS88_07185"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOU07164.1"
                     /translation="RQSNREGQSNQGGQSTRPFFVPLIGLSGDTNPNLQINTIIDRFS
                     SSFQSKGLFINLSSVREKGRALSNIEEHRIQALQEYWKQVEAAVVGLGTPPAFSINIL
                     DELPEKYKEELKKSSSCADILAQFFDENGTIFHSEHEYDLLAYDIRDLPNLQRSVCLA
                     GGELKVPGIVAAAQARFISDLVTDEQTALAILRLLRGQSGQSAEGLPINKKNQKASQI
                     GPIGQSQKGNKS"
     assembly_gap    4794..4893
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4894..5384)
                     /locus_tag="PLS88_07190"
     CDS             complement(<4894..5384)
                     /locus_tag="PLS88_07190"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOU07165.1"
                     /translation="MKTGPDISTIYRIARYYYADGLSQEEIAAKEGFSRSQISRLLEK
                     AKSLGMVRITLVPPASQQSEELSEILAEMMGLRSVLVVPIAKTANRDEIVGAIATRAA
                     DFLGELLPSFNVVGIGWGKTVYETSMLLSRHVGQSDYDRQSNHNRQSNREGQSNQGGQ
                     STRP"
     gene            complement(5539..6267)
                     /locus_tag="PLS88_07195"
     CDS             complement(5539..6267)
                     /locus_tag="PLS88_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07166.1"
                     /translation="MTNGLKRNVERLFDDKGHVLLLALDHAQSGVMPGLENISSLMDK
                     LAFAPLDGFILNIGLAGNLAHSPFLRKKLVLRTSFGASSLASSYPQAHLNHVSPETAL
                     AVGADAVLMMALIGGEDYCSLQPLAADIDAFHQYSIPVVVEIIAAEFDKTATFDVQYH
                     GARIAAELGADAVKVFYVNDFEKVIECCPVPVILAGGPKDRDIGTVAKHAVDCGARGF
                     AFGRNIFQANDPLEVIGSLREILG"
     gene            7021..8679
                     /locus_tag="PLS88_07200"
     CDS             7021..8679
                     /locus_tag="PLS88_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOU07167.1"
                     /translation="MLARLRNEPDEREWLEFKDSFYDPQTIGEYLSALANGACLAGES
                     RGYLVFGIEDKSHEIVSTRFYPYREKARGNQDLIIWLSSGLDPKVGFNIYELNHPKGH
                     VVIFEIISAWNRPIKFHGKAFIRVGASKTELYKYPEKERAILTRRIDWSAMIREGASV
                     EDLDAEAIKKAREQFMIKHPNRASEVKSWDDMAFLNKCKLTIHNAITNTALILLGKQE
                     SSALLSPVIMKITWILKDLNNNALDYEHFAPPILLQVDRILARIRNLIVRALPSGTLF
                     PQEISQYDPWVLREALHNAIAHQDYGLGGRINIVEMPSSILIENVGGFLPGDLDTVIC
                     QDAPLEIYRNPFLAEAMVNLNMIDTQGGGIKRMFEKQKQRFFPMPDYDLSNSEKVKVT
                     IPGNILNENYSRLLMERADLDLWQVILLDRVQKRQRISIEAHRALKSVGLVEGRYPNI
                     IIASKIAGITGEKAKYIRDRGFNKKYYLDMIETLIKEHQPIQRSEIDRLLLDKLPEML
                     TTKQKKAKIHNLMAELVSKNKIENRGSKANPAWYAMNSLQSNNE"
BASE COUNT         2475 a         2078 c         1957 g         2269 t
ORIGIN      
        1 tctccggaat ctcccgaaac cgcgaaccat accttcagct tcgtctgcgc acccgcaggg
       61 atcacgaccg cgatcagtgc gagcatcacg atacatatca tgacagacct tttcatggta
      121 ttacctcctt gaggaattgc tcctctattg ttagcacgca tgcgcgtgcc caattctcta
      181 ttcatgtttc ctgccaacac gcatgcgcgt gccgtatttt ccattcatgt cttgagtccg
      241 gtcaacgtta tcgattgtac aaactctttt tccatgagca tgtacactat caatacaggc
      301 actatggcca ttaccgaggc cgccatcagc acttgatatt ccgtaccggt ttcggttacg
      361 aggtatttga gccctattgg caaagtcctc atctttacat catttgtgat gatgaggggc
      421 cacatatagc tgcgccaatg agtgaggaat gcaaagagcg atgcggttcc gattgctgtc
      481 ttggagttgg gcagcattat ctcgaggaac actcgaatat acccacagcc atctactttt
      541 gccgcatcta tgagttcgtt cgggattgtc tggaaaaacg acattataag cagcgtcgta
      601 aaggcgcagt tcaaccccgg tatgataagc ccggtgtagg tatctattaa acctgcactt
      661 gcagccagca ggaatagcgg tatgatagtg acgatctccg gaacgaaaag gctgctttgc
      721 atgaatgcga agagtttgtt cttgagaggg aattcgagct tggcgaatgc gaatgctccc
      781 atcgccgaaa tcgctatctg gagaacagtc tgaactacag agacgaagat gctgttcgca
      841 tagaatcgaa taaaggggat cttcttgaac acttcttcgt aatttgtgaa tttgaatgaa
      901 cttggaatcc atttcggagg atagctatat acatcggctg gcgtcttgaa cgaagagagc
      961 atcatccaga caaatgggaa aatcatgagc gcagaactta tgaaaagaaa gagcatcgta
     1021 aatgggtagc gcgatttcaa atgtttgctc atatcccagt cctccgatta gtcaaagctg
     1081 atctctttgc gccccaatcg ccgctgaatc attgtgaacg caaagattat caagaacagg
     1141 ataagggacg atgccgctcc gcgccccatc ttgcctcttg tgaatgattg gtcgtagata
     1201 tacgagacca tgaggagagt cgactctagg ggaccatggt ttggcgtcat gacatccact
     1261 tcggtgaata ctttgaagca gttgatgatt cccatcatga tgataaaaga agtcacggga
     1321 gaaagaagcg gaagctttac cttgaagaaa acctgccact cgctcgctcc atcgatacgg
     1381 gcggcttcca ggtaagattc cgggatactt tgaagcccgg ccaagaacag aatgatattg
     1441 tagccaattc ctttccatat cgaaaagaga atgaccgaga gcatggcagt cttctcgctg
     1501 tagagccact gcattgaagg taggccaaga aagttcagta tttggtttaa aggaccaatg
     1561 ccaggatcgt aaaaccatag ccagataagg ctcgaagcag ttatgggcac cacaactggg
     1621 gcaaagtgcg caatgcggaa gaagcgcctg aaatggattt tcctgtccag aagcaccgct
     1681 atgaaaagcg caagacccgt gtccaacaca agcttcagcg cagtatagta tgccgtgttc
     1741 ttgaaagcct tccagaaacg gggatccttc agcacgaaga caaaattgcg aaatcccaca
     1801 taggagcgag cggtcgaagt catggtccaa tttgtaaagc tcattatgat tacaagaatt
     1861 acaggaagca aaaacagtag aatgagcgta atgagaagag gcgtaacaaa gatcgccgct
     1921 gccctgtgct gttccttttt gatgctatat acctgcgctc cctttttctt ttttaccgac
     1981 ataggacacc ctctggttct gaattatgat ccctaggaac tagaacttcc gctacatcga
     2041 tgcgaagttt gaagctcacg acttcgcctt ctctatgaaa gggattgcca tctacgacga
     2101 tatcgccatc gcggcttagc ccttctattt caacaaaatc ctttggccca aagagcagat
     2161 gctcggaaag cgccatattc tcgaaacccc gcagaggcgt cgccgctctc acaatgaccg
     2221 tgtcgaaaag cccaagagcc gcggcgccat ccatgtatga ggcattgcag agtgcgcccg
     2281 caatggctct tgcaaagaat tcgcgctttc ttagcggcga agcgattatc tgtgcgggct
     2341 tctgcatgta attttcgatc cgaaggccat tttttcttat gcagaaatcc ctgctcacga
     2401 tgtcggagga aggctcgatt tcctgtttcc tgccatcgtc cagcagggct tttgcggaga
     2461 gattgacaac gcgcccctct agagtgccta aaaacgaatt gccgatgatg gcatcgttca
     2521 gagcataagc gaggtgtttt ccatcgacag agatctcgac cgcatctatt gtctttacga
     2581 aaagcttcga gatgtcgagc ataggaatat cttcgatccc gaaagcgact ataggcccta
     2641 catttatggt ccctgcgcca atgcctagaa gccgcacttt gttgccatga cttatcattg
     2701 catcgagcac ataagaacca agcccatcgc cgcctatgca gatgagcata tccgagcccc
     2761 aatccagaag gctcgccact gaagtcttca gattttccac atagctcgcc gataattccg
     2821 gctttaacct cgaccatgag gagggcagta agtcgcatcc aaatgggccg gcgcatacgg
     2881 cgacatcata agattggaga ctggggacta gcgaaataat ggcttcaggt gcgaatccct
     2941 tacccgaagc agatcctatg acaactccga ttttcatgcc gctcctcttt gtatgtcgct
     3001 tttctttatg ccttattccc tttgtgcttc attttctttg tgtgccattc ccttacggtc
     3061 taagcacgat tttcaatgcc tcgcccgagg caagcatttc gaatgcctct tttccgagct
     3121 ccagcgggta attatgcgtg attatcttcg aaaacatctc cttatgcgct tctatcaagg
     3181 agagagcttc cttgaaatgg cggagcgcat acccaaaact ccctttcaga gagacctgtc
     3241 gatagtggat cgaataagca tcgatggcga ctatttcgtc gctcgagagc cccgagaaca
     3301 tcaagacagt gcctgcgtcg gctactagct ttatatattc gcttacgagc tcttttttgt
     3361 tgacggcgat gatggcgata tcgtagaagc ctttctccac ccggcctggg cgggcgcttt
     3421 ccacttgtcc agggcgggca ctttccacct ggcctgcgcg ggggctttcc acatggccag
     3481 caccgccagg ctcgaaagct tcgatgcccc atgaggcgat tctctctctt ctctgcgcgc
     3541 taggctcgac aacggcaacc ggtatgcctt ttgccctgaa aagaagcgca aagagagcgc
     3601 ccatcggccc cgccccgacc actagagccc tgcttgtcgg ctttagatcg agctgttcga
     3661 atccatttag aacacaggcg agcggctcga ccaaggtata gacatcgtct agctctttga
     3721 tatggaatac gccttgctcg atataatccg atgctatccc caccttctcg cagaatgcgc
     3781 ctccttcgac ataatgcttg ttagcgcata gagatcccgc accccttatg cagacatcgc
     3841 aaaaaccaca ttctgcataa ggcgcaacaa ccaccttgtc gcccgcaaaa tagccgcatc
     3901 ctataggagc cttctcgact aggccataga attcgtgtcc caatatcgca ggaggagaaa
     3961 agagatgatg tcccttctga tatgttttaa gatcggttcc gcaaatgccg cagccaagaa
     4021 ctttgacgat aaaatccgag cttggctccg gaacatcttt tacttcgagc tgtttgatgc
     4081 cagtgtatac aagtgccttc atgatttatt tcctttttgt gattggccga taggcccaat
     4141 ttgacttgct ttttggttct tcttgtttat gggcaagccc tctgcggatt gtccggattg
     4201 cccacggaga agtcttagaa tcgcgagggc agtctgctcg tccgtgacca gatcgctgat
     4261 gaacctcgct tgtgcagccg cgacaatacc cggaaccttc agctcgcccc cggccaggca
     4321 gaccgatcgc tgtagattgg gcaagtctct tatatcgtaa gcgagcaagt cgtattcatg
     4381 ttcggagtga aaaatagtcc cgttttcgtc gaagaattgg gcaagaatat cggcgcatga
     4441 agagctcttt ttgagctctt ctttgtattt ctccggcaat tcgtcgagga tgtttatcga
     4501 aaaggcaggg ggagttccca accccactac agcggcctct acctgcttcc agtattcttg
     4561 gagcgcctgg atgcgatgct cctcgatatt ggagagcgct ctgccttttt cgcgcaccga
     4621 cgataggttg atgaacagcc ccttggattg aaaagaactg ctgaaacggt cgattatggt
     4681 gttgatctgc aaattcgggt tggtatctcc cgaaaggccg ataagaggca cgaagaatgg
     4741 gcgcgtactc tgtccaccct gattgctctg tccttcacga ttgctttgtc gatnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngggcgcg tactctgtcc accctgattg
     4921 ctctgtcctt cacgattgct ttgtcgattg tgattgcttt gtcgatcata atcgctctgt
     4981 ccaacatggc gagatagaag catcgaggtt tcatagacgg tcttgcccca gccgatcccc
     5041 acgacattga aagaaggcaa tagttcgcca agaaaatcgg ccgctcgcgt agctatcgct
     5101 ccgacaattt catctcgatt ggcggtcttg gctatcggaa ctacaaggac tgacctaagt
     5161 cccatcatct cggcgagaat ctccgacaac tcttcgcttt gctgactcgc cggaggcaca
     5221 agcgtgattc ttaccatacc cagcgacttg gccttctcca ggagtctcga aatctgcgaa
     5281 cgagaaaagc cttccttggc tgctatttct tcttgagaaa gcccatccgc atagtaatat
     5341 cgggctattc tatatatcgt ggagatatca ggcccggttt tcatcgaaaa tgccatcctc
     5401 ctcttaagcg ctcatcctat aaaatgccta ttccagcagc gccaatctaa atctcataag
     5461 gggctagcca gtcaaatgcc tattctgacg agccatcatc aaaaaggcgc tgccagccaa
     5521 gggcctattc cagcaaccct atcccaaaat ctccctcaaa gacccaatca cttccagagg
     5581 atcgtttgct tggaaaatat ttctgccaaa ggcaaatcca cgcgctccgc aatccactgc
     5641 atgcttggct acggtgccga tatccctatc ctttgggccg cctgcaagta tgactggaac
     5701 tgggcaacat tcgatcacct tctcgaagtc gtttacatag aagaccttca ccgcatctgc
     5761 ccccaattcg gcggcaattc ttgcgccatg atattggaca tcgaaagttg cggtcttgtc
     5821 gaactccgcg gcgattatct cgaccacaac cggaatggaa tattgatgga atgcatctat
     5881 atcggccgca agaggctgta gactgcagta atcctcgcca cctatcaaag ccatcatgag
     5941 aacagcatct gcaccaacag ccagcgccgt ctcagggctc acatgattga gatgagcctg
     6001 cggatagcta cttgcaaggc tcgaagcgcc aaaggaagtc ctcaatacca gttttttcct
     6061 aagaaaagga gagtgtgcaa gatttcctgc cagaccgata ttcagaataa agccatctag
     6121 aggcgcaaaa gcgagcttat ccatcaagct cgaaatgttc tcaagcccag gcattacccc
     6181 gctctgcgca tgatcgagcg ccaataataa gacatgcccc ttatcatcga aaagcctttc
     6241 gacatttctt ttaagaccat ttgtcaatct atttctcctt cttgatttgg tggccttgtt
     6301 acacggagat tgccgttttc ctgctcgctg gcgctattaa ccaagccaac tgcataatgc
     6361 aactgcaagc cagagcgaca aagcttgctc cgactaagac cataatgcaa ctgtaagcta
     6421 taagcctaat gcgtgctaag accataatgc aactttgtgc atcaccaaat cgcatataaa
     6481 taggatacat cgagttttca tagctgtcaa caaaaaacaa tggggtattg gatataggaa
     6541 ttcagataag tactattcga caatccatga tagggtaatt gaagagagct ctaggctgaa
     6601 catatcatag agccattgct acattagaat tagccaaatt gtctttataa tcaatttttc
     6661 aaagaagact ttttgaaact ttcagattaa agatccttat ctacagcttt gcgattctga
     6721 atcttaaaaa gaaggccaaa gatcgaaatg tgagataaaa acgattttac attgattttg
     6781 gccccaaaat ttatagcgag agcgaagaaa gacttgatcc taatttaatc atttatggat
     6841 cggcctcggc aagacattga gacagaatta tgctgatatc tttatctact gcaggatttt
     6901 aattaattaa ggacttgttt tgaagtttta agataggaaa tactttcagg ttagcatgat
     6961 aaagaggtta gcgaatttca aaaggagacg aaaagaatag ctgaggaaga ccttttttct
     7021 ttgcttgctc gacttagaaa tgaaccagac gaaagagaat ggcttgaatt caaagatagt
     7081 ttttatgatc cccagacaat tggagaatat ctttccgcct tggctaatgg cgcttgtcta
     7141 gcaggagaat ctcggggcta tctagttttt ggtatagaag ataaaagcca tgagatagtt
     7201 agtactcgct tttatccata tagagaaaaa gcaagaggga atcaagactt aataatatgg
     7261 ttatctagtg gcttggatcc aaaggttgga tttaatattt atgagctaaa tcatcctaaa
     7321 ggccatgtcg taatcttcga aataatatca gcttggaatc gtccaattaa atttcatgga
     7381 aaagccttta tccgtgtagg agcaagcaaa acagagcttt acaagtatcc agaaaaagag
     7441 agagcgatat tgacaaggag aatagattgg tcagcaatga tccgagaagg agcaagcgtc
     7501 gaagatctcg atgctgaggc tataaagaaa gctcgtgagc aattcatgat aaaacatcca
     7561 aaccgagcat cagaagtaaa atcctgggat gatatggcat ttctcaacaa atgcaagctt
     7621 actattcata atgctataac caatacagct cttatccttc tggggaaaca ggaatcatcg
     7681 gcgctcttat ctccagtaat catgaaaatc acatggattt taaaagatct aaataataat
     7741 gcattggact atgagcattt cgcacctcca atactgcttc aagtagatcg gatcctagct
     7801 cgaatcagga acttgatagt cagggctttg ccaagcggaa ctcttttccc acaagaaatt
     7861 agtcagtacg atccttgggt cctacgcgag gcattacata atgctatcgc gcatcaagat
     7921 tatggccttg gaggacgaat taatatcgtt gaaatgcctt cttcaatctt gattgagaat
     7981 gtcggaggat ttctaccagg agatttggat actgttatct gccaagatgc accgctagaa
     8041 atctatcgga atccatttct tgcagaagca atggtcaact taaacatgat tgatacccag
     8101 ggcggaggaa ttaaaagaat gttcgagaaa caaaaacaac gattctttcc aatgcccgac
     8161 tatgatctct ctaattcaga aaaagtaaaa gtaacaattc ctggcaatat tctaaacgag
     8221 aactatagtc gattgctaat ggaaagagca gatctcgatc tttggcaggt tatccttctt
     8281 gatcgagttc agaagcgaca gcgcataagt attgaagcac atcgagctct gaagagtgta
     8341 ggcttggtgg aaggaagata tcccaatatt attattgctt caaagatagc tgggattaca
     8401 ggagaaaaag caaaatatat tcgagataga ggcttcaata aaaaatatta cctagatatg
     8461 atagaaacct tgattaaaga acatcaacca atccaaaggt cagaaataga taggcttctt
     8521 ttggacaagc tgccagaaat gcttaccact aagcaaaaaa aggctaaaat ccataatctt
     8581 atggccgaac ttgtcagcaa gaataagatt gagaacaggg gaagcaaagc aaatcctgca
     8641 tggtatgcaa tgaatagctt acaatcaaac aatgaataat taagcgaaca agtgtcaatt
     8701 tttctcaata aaaatgataa ttcaatattc aataatatat tatcctttgt ttgatttttt
     8761 ccattaagaa tgattaacaa aggtctaaac aaagtaaaaa cggcaagctc caataataaa
     8821 acaatactgc tacgaacctt gtataagaat ttgaagcata aagtagggaa gagagcgat
//
LOCUS       DAOSOB010000066         8535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_8231_length_8535_cov_71.452241, whole genome shotgun sequence.
ACCESSION   DAOSOB010000066 DAOSOB010000000
VERSION     DAOSOB010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8231_length_8535_cov_71.452241"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLS88_07205"
     CDS             <1..453
                     /locus_tag="PLS88_07205"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOU07168.1"
                     /translation="KIANPMTENYEYVRNSPIPGLLQTESVSAKASYPHRTFEVGKVA
                     LKDAEANYGIVTRQYIGFLVSHAQADYNEIASHVATLMYFLGKNYTVRESDDPRFVPG
                     RQVEILVGEKDSKRIGVFGEVHPAVLEAFDVMMPCATAELDLDLLLTS"
     gene            522..2162
                     /locus_tag="PLS88_07210"
     CDS             522..2162
                     /locus_tag="PLS88_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOU07169.1"
                     /translation="MDLYAHEPLGFDGILVHIEADIRSGIPAVEIVGLASTSVREARE
                     RARIAVRNAGFEFPQDRILVNLSPADLPKEGSAYDLPIAIKILEKSGQVGDLPICALA
                     MGELTLEGEIRPVRGILPAVRSAASHGIRICIIPFENAQEARIVKSVQVWPIRHLAES
                     ARILIEIGKGKTPQGQTLQCQEEDILQQSSSFWFDLNESKETYSDFRGDERLMRALVI
                     AAAGGHNMFLAGPPGAGKTMAARRFPSILPDLSEHEALDTASIYSLWGSLQTCLGKRP
                     PFRSPHHSASLEGILGGARPLRPGEVSLAHHGVLFLDECPEFRRDVLQGLREPVERGY
                     VDIVRAGRVLRFPSDFQLIMAANPCPCGNLGMPGKTCICSPEEIRRYWKKLGGPLLDR
                     IDMRIAILPPKASRLIESQAVDVDSLRDKVLFARDLQSIRLKRINDDGTNKKEMDSIG
                     YSRCITFSDTKQILARNNAHIPAGIINEMCHVEGSAEKAFLSGMANYNLSARAGHSIL
                     RLARTIADIDGEDEISESAIQEAIEYRQFGDGDAVWPF"
     gene            complement(2256..3332)
                     /locus_tag="PLS88_07215"
     CDS             complement(2256..3332)
                     /locus_tag="PLS88_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU07170.1"
                     /translation="MQNKVRFGMIGYGKVAALHAKALATIQHGELVSVCGHRQEKRDS
                     FAYRWGLNSRNSVKEMVKEDAIQAVLITTPHPQHYSDALLALKAGCHVLVEKPLTLSV
                     AEAETLISEANKRNLLLSVIAQRRWYPSCQRIRHAIDEGNLGIPALGQLTILGWRDEA
                     YYRSDPWRGSWEHEGGGVIVNQAPHQFDLLCWYMGEVAEVYGRWSNINHPYIEVDDSA
                     VATVLFKSGGLASIFISNSQKPGIYAKVHIHGSSGASAGVQTDGGAMFIAGRSAVLEA
                     PYNDIWTIPGQEALRTEWQKEDEEFFSSIDATWYFFAQQEENFARAILEGQAPSVTGS
                     DGLQVARIIEGIYRSNREGIPIRY"
     gene            complement(3447..4481)
                     /locus_tag="PLS88_07220"
     CDS             complement(3447..4481)
                     /locus_tag="PLS88_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOU07171.1"
                     /translation="MKAMVLKEYKYLEIEDIPIPHIEAADELLVRVKATAICGSDIHG
                     FDGSTGRRKPPLVMGHEASGVVESVGSEVKSLKIGDRVTFDSTVWCGECYFCRQGQVN
                     LCDKRRVLGVSCDEYRKDGTFAEYVIVPERIVYKLPDNVAFEEAALTEPVSVAMHAFD
                     ITGMKPGENAAVIGSGLIGLLLIQIIRAYSPHLIIAFDTDEKRRKAAIEAGADIALDP
                     QDPESHKTVLSMTNNRGVDRAFEAVGASAAIGTAIESVRKGGSVTLIGNISNKAEIPL
                     QSVVTRQIALLGSCAISGEYPVSLALMANRTIDVRQVISAIAPLEEGPLWFERLYKRE
                     PGLLKVVLTP"
     gene            complement(4511..5596)
                     /locus_tag="PLS88_07225"
     CDS             complement(4511..5596)
                     /locus_tag="PLS88_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOU07172.1"
                     /translation="MNPIILLPEEKAKLDLLSLGGLVMRMDPGIVPFAFAEHYDVHVS
                     GGEYNVAANLARCFGKRTAIVSAIVDNPLGEKVAAEVRKMNVLGKYKHFKSDGVRGPN
                     MALVWSDRGQGVRPPLVFYNRANEAAALLKPGDFDFAAIFDEGVRWFHSGGIFAALSD
                     STPKLIIEAMKIAHDHGAIVSFDLNYRAKLWAAANTGEKPSDVFGRIVEHVDVLLGNE
                     EDLQMGLGLPGPDIHSASKLDPSSFLKTIEQVHQRWPNIKAVATTLRDVKSTNRHLWS
                     AVLWIEGAHWVAPTMELDIYDRVGGGDGFASGLIYGLLDGADPEEALRLGWAHGALLT
                     TYPGDTTMASLEQVKALAQGGSARIQR"
     gene            complement(5646..7097)
                     /gene="gnd"
                     /locus_tag="PLS88_07230"
     CDS             complement(5646..7097)
                     /gene="gnd"
                     /locus_tag="PLS88_07230"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOU07173.1"
                     /translation="MKADIGLVGLAVMGENLVLNMESRGFTVAVYNRTIEKVDSFLHG
                     RGAAKRILGAHSPKEFVEMLKKPRIVMLMVRAGTAVDEMIAQLVPFLEPGDIIVDGGN
                     SNYEDTMRRVSDLESKGFLFVGTGVSGGEEGALSGPSIMPGGSEAAWPHIKPIFQAIA
                     AKVDDGSPCCEWVGPSGAGHFVKMVHNGIEYGDMQLISEVYQIMRDRLRMTPDEMAMV
                     FDRWNEGELDSYLTSITSDILRFKDEDGSPLIEKILDAAGQKGTGKWAGINALNFSVP
                     LTLIVEAVFARCISAMKDERLRAVKVLGKPIGTEFSGSQEKTIEDLGAALYAAKIISY
                     AQGFMLMREASAAYNWKLNYGSIALMWRGGCIIRSRFLGRIKEAFDADPELSNLAFAP
                     FFAEQLLKNEKAMRNVVAEAVVGGIPVPALSSALAWFDALRTERLPANLIQAQRDYFG
                     AHTYERIDKPRGQFFHTNWTGHGGSTSASTYIV"
     gene            complement(7141..7992)
                     /locus_tag="PLS88_07235"
     CDS             complement(7141..7992)
                     /locus_tag="PLS88_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOU07174.1"
                     /translation="MERSTAPSCLYLIHSLSNSLSERDKRIADYILNDPAKAVHFSIE
                     ELAEAAHVSVSTLVRFVKKLGFRGYQQFRITLASEALAPEARIYETIVDSGEDPIHLA
                     FDSASKALEITASMIDTQALNELASRIIRANCVYLFGLGGSLIVAKDALHKLVRTGIR
                     CIGAEDYHVQLMIASQMNQQDTAILISHTGANKDAIGLAETIKATGAFLCVITTYPRS
                     ALSRMADMRFISASSGSQAISEAFSARIAQLALIDSLYIAIMKQLGEEGIKNVEKMRA
                     TIAKRRI"
     gene            8216..>8535
                     /locus_tag="PLS88_07240"
     CDS             8216..>8535
                     /locus_tag="PLS88_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU07175.1"
                     /translation="MKRKFMFALVALLALFATSAQQKEIRVLLANHPYGDLLKAAIPE
                     YEKATGVKVNVESLQESQLTTKLTTEFATKSSTVDVFMTRPLQEGKMFYKNGWYEPLT
                     GYDFS"
BASE COUNT         2242 a         2054 c         1893 g         2346 t
ORIGIN      
        1 aaaatagcca atcctatgac cgaaaactat gagtatgtca ggaattcgcc tattccaggg
       61 ctcttgcaga ctgaaagcgt atcggccaaa gcgtcatatc ctcatcggac attcgaagta
      121 ggtaaagttg cgctcaagga tgctgaagca aattatggta ttgtcacacg acaatacatt
      181 ggctttttag taagccatgc acaagcagat tacaatgaaa tcgcttctca tgtcgcgaca
      241 cttatgtatt ttttgggcaa gaattacaca gtacgcgagt ccgatgatcc gcggtttgtg
      301 ccaggccgac aggtcgaaat ccttgttggc gaaaaggatt cgaaaaggat aggggttttc
      361 ggcgaagtgc accctgcagt actggaagcc ttcgatgtaa tgatgccttg tgccacagct
      421 gaacttgacc tggatttact tctgactagc taagagaatt gaagcgcaat gccgttttct
      481 ctgcgtcttt aagagagaga caaagagagg ctaaaaacgg catggatctc tatgcacatg
      541 aaccgcttgg attcgatgga atcttagttc atattgaggc agacatcaga agcggaatac
      601 cagcagtgga aatagttggt ctcgcatcga catcggtgcg tgaagcacgc gagcgcgcca
      661 ggattgcagt aaggaatgcg ggctttgaat ttcctcagga tcgtattctc gtcaaccttt
      721 ctcctgcgga tttgcctaaa gagggctcag cctatgattt gcctattgca ataaaaatac
      781 tcgaaaaatc tgggcaggtt ggagacttgc cgatatgtgc gctggccatg ggagaattaa
      841 cgcttgaagg agaaatacgg cctgttagag ggatcttgcc agctgttcgc tctgccgctt
      901 ctcatggtat tcgaatttgt atcatccctt ttgaaaatgc ccaagaggca cggattgtaa
      961 agtctgttca ggtatggcct atccgtcatc ttgcagaatc agcaagaatt cttatcgaaa
     1021 ttggaaaagg aaaaaccccg caaggacaga cactacaatg ccaggaagaa gatattttgc
     1081 agcaatcttc ctctttttgg ttcgatctga atgaaagcaa ggaaacatac agcgattttc
     1141 gaggtgatga gagattgatg cgtgcacttg tcattgctgc ggcgggagga cataacatgt
     1201 ttctcgccgg accaccaggt gccggaaaga ccatggctgc tcgtcgattt ccttcgatcc
     1261 ttccagatct ttcagagcat gaagctctag atacagcttc tatttattct ctttggggga
     1321 gcctccagac ctgcttggga aagaggccac catttagatc accacaccat tctgccagtc
     1381 ttgaaggaat actgggaggt gcaagacccc tgaggccagg agaagttagt ctggctcatc
     1441 atggagtgct ttttctcgac gagtgtcctg aatttaggcg ggatgtttta caggggctcc
     1501 gcgagcccgt tgaacgtggc tatgttgata tcgtgcgagc aggtcgagtg cttcgttttc
     1561 cttcggattt tcagcttatt atggcagcaa atccttgtcc ctgtggcaat ttgggcatgc
     1621 ctggaaaaac ctgcatatgc tctcccgaag agattaggcg atattggaaa aagctcggag
     1681 gacctctctt agatagaata gatatgcgaa tcgcgatttt gcctccgaaa gcttcgcgcc
     1741 ttattgagtc tcaagcagtc gatgtagatt cgcttcgcga taaagtttta tttgcacgcg
     1801 atcttcaaag cattcggctc aaaaggataa acgatgatgg aacgaataag aaagaaatgg
     1861 atagtatagg atattcaaga tgcataactt tttcggacac aaaacaaatt cttgcccgca
     1921 acaatgccca tattcctgct gggatcataa atgagatgtg tcatgttgaa ggatcggccg
     1981 aaaaagcatt tctatcggga atggccaatt ataacctttc tgcccgtgct ggtcacagta
     2041 ttcttaggct tgctcgtacc atagccgaca tagatggaga ggacgaaatc tcagagtctg
     2101 caatacaaga agcgatcgag tatcgacagt ttggtgatgg agatgctgtt tggccttttt
     2161 aatcgatgga ttcttgatcc atttaaatcc tatcgatata aatttcgagc tcaaaacata
     2221 aataaagtct ggataaaatc tttgaaagga agtgttcagt accgaattgg tatgccttct
     2281 ctgttagatc tataaattcc ttcgatgatg cgcgcgacct ggaggccatc tgagccagtc
     2341 accgagggag cttgcccctc caaaatcgcg cgagcaaaat tttcttcctg ctgtgcaaaa
     2401 aaataccatg tggcatcgat gctagaaaaa aattcttcat cctctttttg ccactccgtc
     2461 ctaagagctt cttgtccagg aatggtccaa atatcgttat atggcgcttc tagcacggcg
     2521 ctgcgtccag caatgaacat tgctccacca tcggtttgaa ccccagcaga agcccctgaa
     2581 cttccatgaa tatggacttt cgcatagatt cctggctttt gtgagttaga tataaaaatc
     2641 gatgcaagac ccccagactt aaagagaacc gtcgctactg ccgaatcatc gacctctatg
     2701 taaggatgat tgatattcga ccatctaccg tagacttctg ctacttctcc catataccag
     2761 cacaggagat cgaattgatg aggagcctga ttaacgatca cacctccgcc ttcatgttcc
     2821 caggatcctc gccatggatc ggatcgataa taggcttcgt ctcgccagcc gagaatagtg
     2881 agctgaccta aggctggtat cccgagattt ccttcgtcta ttgcatggcg aattcgctga
     2941 caagatggat accagcgccg ctgtgcaata acactgagaa gcagatttcg cttatttgct
     3001 tcgctgatca gtgtctcggc ttcggctact gatagagtaa gaggtttttc gacaagcaca
     3061 tggcatcctg ctttaagcgc taataacgcg tccgaataat gctgggggtg gggtgttgta
     3121 atcaagacag cttgaatggc atcttctttg accatctctt tcacgctatt tcgggaattc
     3181 aacccccatc gataggcaaa agaatctcgc ttttcttgtc tatgaccaca aacagataca
     3241 agttctccat gctgtatggt tgcgagtgcc tttgcatgga gtgcagcgac ttttccatat
     3301 ccaatcattc caaatcgaac tttattctgc atgcttatct cctatctatg aggaaacctt
     3361 atatctgaga atactttatg agatttgcca aaaacaaaca aaagaaatgg cgcagcaatc
     3421 gatagatcga ttgacacatt catccatcaa ggagtcaaca cgaccttgag aagccccggc
     3481 tcccgcttat atagccgctc aaaccacaac gggccttctt cgagaggagc aatcgcgctt
     3541 attacttgtc tcacatcgat agttcgattc gccatcaatg caagcgaaac tggatactca
     3601 cccgaaatcg cgcaagaacc aagcaatgca atttgccgtg tgacaaccga ctgcaaggga
     3661 atttccgcct tgttgctgat attgcctatt agcgtcaccg aacctccctt tctcacgctt
     3721 tctatggcgg taccaatggc agcgcttgct cccactgcct caaacgctct atcgacacct
     3781 ctattatttg tcattgaaag caccgtttta tgagattctg gatcttgggg gtcgagcgct
     3841 atgtcagcgc ctgcttctat ggctgctttc cttcgctttt catccgtatc gaaggctatt
     3901 atcaggtgtg gcgaatatgc gcggattatc tgaatgagca gtagcccgat aagcccagag
     3961 ccaataactg ctgcgttttc tcctggtttc atgcctgtga tatcgaatgc gtgcatggca
     4021 acgctgacgg gctcggtgag tgcggcttcc tcaaatgcta cattgtctgg caatttatac
     4081 acaattcttt ccggaacaat aacatattct gcaaaagttc catcttttcg gtattcatca
     4141 caagatactc cgagtacacg ccttttgtcg cacaaattta cctgtccttg tctgcaaaag
     4201 tagcattcgc cacaccacac cgtcgaatca aaggttactc tatcacctat tttaagcgac
     4261 ttgacttcag atcccactga ttcaactacg ccggaagctt catgtcccat cactaacgga
     4321 ggctttcgcc ttcctgttga accatcgaaa ccatgaatat cggaaccaca gattgccgta
     4381 gcctttactc gaacgagcag ttcatcggcg gcttcgatat gtggaattgg aatatcttct
     4441 atttccaagt atttgtattc tttcaatacc attgccttca tagttgctat taattcctat
     4501 tgtatttctt ttatctctga atgcgtgctg aaccgccttg tgcgagcgct ttcacttgct
     4561 caagacttgc catcgtggta tcgccaggat aagtagtcaa aagcgcacca tgcgcccatc
     4621 ctagtcgaag agcctcttct ggatcagccc cgtcgagcag tccatagata aggccagaag
     4681 caaatccatc tcctccacca actcgatcat agatatcgag ctccatcgta ggagcaaccc
     4741 aatgagcccc ttctatccag agcactgcgc tccaaagatg tctattagtc gatttgacat
     4801 cacgcaatgt tgttgcaacg gccttgatat tgggccatct ttggtgaacc tgctcgatgg
     4861 ttttgaggaa ggaagaagga tcgagtttcg acgcagaatg gatgtctggg cctggcaagc
     4921 caagtcccat ctgtagatct tcctcattac ctaaaaggac atcgacatgt tcgacaattc
     4981 tgccgaacac atccgaaggc ttttcgccag tattcgctgc agcccaaagc tttgcccgat
     5041 aattgaggtc gaaagatact attgctccgt gatcatgggc tattttcatg gcttcgataa
     5101 ttagcttagg tgttgaatcg gacaaagctg cgaagatgcc tccagaatgg aaccatctga
     5161 cgccttcgtc aaagattgct gcaaaatcga aatcgcctgg ttttagtaat gctgccgctt
     5221 cattggcgcg gttgtagaat actaggggtg gacggactcc ctgtccccga tctgaccata
     5281 cgagagccat gtttgggcct cgcactccat cggatttgaa atgcttatac ttgcctagga
     5341 cattcatttt ccgaacttct gcagcaacct tttcgcccaa ggggttatcg actatagcac
     5401 ttacgattgc ggttctcttt ccaaagcatc gcgctagatt agcggctaca ttatattctc
     5461 ctcccgaaac atggacatcg taatgctcag caaaggcaaa aggcacaatg ccaggatcca
     5521 ttctcatgac aagacctccc aaggatagaa ggtctagttt cgctttctct tctggaagca
     5581 atatgattgg attcatgctt tcaatctcct catatcatgg taattatgtc agatatctat
     5641 gaatatcaaa ctatatatgt gctcgcagag gtagagcctc catgacctgt ccaatttgta
     5701 tgaaagaact ggccacgagg tttatctatt cgttcataag tgtgtgcacc aaaataatct
     5761 cgctgtgctt ggatgaggtt tgcaggcagt ctctcggttc ttagagcatc gaaccatgca
     5821 agggcagaag atagagctgg cacgggtatt cctccaacta cagcttctgc taccacattt
     5881 ctcattgctt tttcattctt gagaagctgc tcagcaaaga agggagcaaa agccaggttg
     5941 ctcagctcgg gatcggcatc gaaggcttct ttgattctgc caaggaatcg cgaacggatt
     6001 atgcaaccac ctctccacat tagagcaata gagccatagt ttagcttcca attataggcc
     6061 gccgaagcct cgcgcatcag cataaagcct tgagcatatg agattatttt cgctgcatag
     6121 agtgcggcgc ccaagtcttc aatggttttt tcttgcgagc cagagaattc tgtgccaatt
     6181 ggttttccta gcactttgac cgccctaagc cgctcatcct tcatagcaga gatacaacga
     6241 gcaaatacgg cttcgacaat caatgtaagc ggtacactaa aattgagagc gttgattccc
     6301 gcccactttc ccgttccctt ttgtccagct gcatcgagga ttttttctat tagaggactc
     6361 ccgtcttcat ccttaaatcg aagaatatcg cttgtaatgg aagttagata actatcgagc
     6421 tctccttcgt tccatctgtc gaagaccatc gccatttcgt ctggggtcat tcttagtcta
     6481 tctcgcatta tttggtagac ttctgagatg agctgcatgt ctccatattc gataccatta
     6541 tggaccattt tgacgaagtg tccggctccg ctcggtccaa cccattcgca gcaagggctt
     6601 ccatcatcta cctttgcagc aatggcttga aaaattggct taatatgagg ccaagctgct
     6661 tcggagcctc ctggcatgat cgaaggacca ctaagagctc cctcttcacc tcctgaaact
     6721 ccagtaccca caaataagaa gccttttgat tctaaatcgg aaacgcgcct catcgtatcc
     6781 tcataatttg agtttcctcc atcgacgatg atgtctccag gctccaagaa gggcaccagc
     6841 tgtgcgatca tttcatctac agcagtccct gctcgtacca ttagcattac aattctgggt
     6901 ttcttgagca tttctacaaa ctcttttgga gaatgagcac caaggattcg ctttgctgct
     6961 cctctgccat ggaggaaaga atctactttt tcaattgttc ggttgtagac tgctactgta
     7021 aatccgcggc tttccatgtt gagcacgaga ttttcgccca tcaccgcgag tccaactagg
     7081 cctatgtcgg ctttcataca tcctccccta tctcccctat gattagatgc gcccctatgg
     7141 tcagatacgc cgctttgcga tggtagcgcg cattttttct acattcttta tgccttcttc
     7201 tcccagctgc ttcattattg caatatagag cgaatctatt aaagcgagct gcgcgattct
     7261 ggcagagaaa gcttccgata tggcctgaga cccagaggat gccgatataa agcgcatgtc
     7321 agccatccta gaaagggcag accgtggata agtggtgatc acacatagaa aagctcccgt
     7381 agccttgatt gtctcggcaa gtccgatagc gtctttattg gctccggtat gcgatattag
     7441 gattgccgta tcctgctgat tcatctgtga agctatcatg agctgcacat gataatcctc
     7501 agcgcctatg caacggattc ccgtgcgaac aagcttatgc aatgcatctt ttgcaactat
     7561 caaggatcct cctagaccga aaaggtagac gcagttagct ctaataatgc gcgaagccaa
     7621 ttcattcaaa gcctgggtgt caatcatcga tgcagtgatt tcgagtgctt tcgaagcaga
     7681 atcgaaagcc agatgaatag gatcttctcc gctatcgact atggtttcgt aaatccttgc
     7741 ctccggtgcg agcgcttccg aagccaaggt tattctgaat tgctgataac ctctaaagcc
     7801 aagtttcttt acaaagcgca caagcgtcga gacggaaacg tgggcagctt ctgccaattc
     7861 ttcgatactg aaatgcaccg ccttggctgg atcattcaga atataatccg cgatgcgctt
     7921 atcgcgctcg ctcagtgagt ttgagagcga atgaattaga tatagacagc tcggcgcagt
     7981 ggatctttcc atagaaatct cgtcaagtct tctcattcta tattatatgc tcggatgagt
     8041 acagaggagt ggcaaaaatt tccaaccatt tgaaaaaaaa ttaccttcat atgttgacaa
     8101 aaggctcttt tggttcatag tataaaagtt gtcaaatccg tctaacttga atcaatcctc
     8161 aagcgaaagg cggataatta aagaattgca acacatttct atggaggagg ttatgatgaa
     8221 aagaaaattt atgtttgctt tggtggcttt gcttgctctt tttgccacta gtgctcagca
     8281 aaaggaaatc cgagtcttac ttgcaaacca cccctatggc gatctactca aagcggccat
     8341 tcccgaatat gaaaaagcaa ctggagttaa ggtcaatgtg gaaagccttc aagagagtca
     8401 gctcacgacc aaactgacca cagaatttgc aaccaagtct tccacagtag atgtctttat
     8461 gacgagacct ctgcaggaag gaaagatgtt ctacaagaac ggttggtacg aaccactcac
     8521 tggatatgat ttctc
//
LOCUS       DAOSOB010000067         8481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_8305_length_8481_cov_63.340375, whole genome shotgun sequence.
ACCESSION   DAOSOB010000067 DAOSOB010000000
VERSION     DAOSOB010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8305_length_8481_cov_63.340375"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLS88_07245"
     CDS             <1..330
                     /locus_tag="PLS88_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07176.1"
                     /translation="YQRLAGQLLRTRHSYLQLEDPEFCPVYAFPIFSETSVKEIIDYA
                     MKHGVESEYAFASSIAKRISGSEPQEEVIFPTARSIAMRCILFPMHHKLTNQQVDQIG
                     KVIATLP"
     gene            340..1194
                     /locus_tag="PLS88_07250"
     CDS             340..1194
                     /locus_tag="PLS88_07250"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOU07177.1"
                     /translation="MSESKSTLIFANLNKDDAGHAAQIVQAELEKKGWHAEILAFRGS
                     ISEYPDFDRYSLIISLGGDGTLLYAARFAGPRSIPILPINLGTLGFIAANKMDTWALT
                     FEHWQSGDLKPSKRLMLQSCAYRGKNILHRGIALNDIVVSSESGARMIRMCLSINREC
                     FGYYRADGLIVSTPTGSTAYNLAAGGPALHPEISALIINPICPFTLASRPLVIPASNY
                     IDIEIDETKKAGALLIADGHIVANLEKGDVVQIREYEKSAFLVVPSGNAFFSALRTKL
                     GWSGDNDA"
     gene            1187..2884
                     /gene="recN"
                     /locus_tag="PLS88_07255"
     CDS             1187..2884
                     /gene="recN"
                     /locus_tag="PLS88_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOU07178.1"
                     /translation="MLEQLSVRNFAIIESIDIDFSPGMTVFSGETGTGKSLIVDALGF
                     LLGARVDNSIIREGATECAVSGLFSASSDSQVGNWLKENGIEWQPNEEILLRRVLKQN
                     GRTLAWIQDRQVSRNELLEFTQNLVDIHGQHEHQRLIDSSTHLDMLDAYAGLEDELRS
                     YQKLYLEWKEAARQLQELLEQKAKRAQEVDYLDFVIRDIASIKPKPNEDNELLAEEKI
                     LSEHEKLFGAVSEALMFLDSDESADVLHALKKVRANIETARTIDIRLGSFSERLSTAY
                     YELEDVSESLNEYKASLRFDPDRLESIERRLADLQRLKHKYGPKLDDVLQAYERARRR
                     LESLEHAEENAEELEKKEKTLEKEVFNRAYQLSEKRMIAAVSMSSSIKEIVKNLGMPE
                     AQVNLRLKRIIDERGEYRTTPRGIDEAEFFIAPNPGEPELPLSRIASGGELSRFALAI
                     KAVLASKDEVETLVFDEIDSGIGGQVGVAVGEYLKQLSQYRQVLCVTHLATIAVQADH
                     QGKVEKSVSKGRTGASIRFLSGKERETEIARMLSGSSESEVSRIHAAELLKKAISHD"
     gene            3135..5684
                     /locus_tag="PLS88_07260"
     CDS             3135..5684
                     /locus_tag="PLS88_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU07179.1"
                     /translation="MARYIIVGGVAGGASTAARLRRLNEHAEIVLFERGPYISYANCG
                     LPYYAGETIKERERLFVMTPEKFNAWLNVDVRIRTEVISIDREAKKIRARELDSGREY
                     SLEYNALVLSPGAEPIKPPIPGIEDPRIFTLRSVSDIDHIKEYLDTKRPERIIVVGGG
                     FIGLEMAENLHARGSFVTIVEALDQVMNPIDFEMAALVHQHLKQKNVELYLSSAVLKF
                     EEAGSRIVAVLADGTRLDADMIVLSIGVRPETAIAKAAGLETTPNGAILVNENLQTSD
                     PAIYALGDAIAFPHPVLDMAMPVPLAGPANKQARVVADNIAKGPGTRKWHGAIGTSIA
                     KVFDITVAAAGVAEKLLRRNNIPCISIITHGSSHASYYPGAQPLTIKTIFTPDGTLLG
                     AQVVGYDGVDKRIDLIADYIRRKAKVTELGEIEHAYAPPFSSAKDPVNIAGMVAENVL
                     AGLSRHLQWHEVKAFQEKGGFVLDVRTPEEFSIGAIPGAKNIPLDSLRQNLGEIPQNR
                     EVLIYCGVGLRGYLAERILRQNGWTSIANLSGGYKTWEIATEPQSNKGIYKPGFVGFQ
                     SGLGKGSQELLHTTFAEGSGMPESFAAKSQTIVQVDACGLQCPGPIMRLKTEIDKLPE
                     GGRVVIRSTDPGFVRDAGAWCKVTGNLLISMEESNGTYTAMVEKTAKQSAMAVAQETE
                     GMRGEPFVQMTPKGATIIVFSNDFDRALASFVLANGAAAAGKDVTMFFTFWGLSVIRK
                     PNAPRVAKDFMGRMFGMMLPKHAGGLSLSHMNFGGIGPKLMKSRMKSKNIDMLETMMS
                     QARQAGVHFIACQMSMDIMGVKHEELLDGVEVGGVATYMEAATEGNVNLFI"
     gene            complement(5884..6318)
                     /gene="cdd"
                     /locus_tag="PLS88_07265"
     CDS             complement(5884..6318)
                     /gene="cdd"
                     /locus_tag="PLS88_07265"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOU07180.1"
                     /translation="MKTEYASLLEQALKACEQSYAPYSKFRVGAALLCEDDSIITGVN
                     VENRSFGLTICAERNAIAAAIAAGKKKFKALVIATPDADYPVSPCGACRQVISEFMPP
                     SATVIFGNSEDSYISSTVGELLPYDALHELASQEQDLKVSKN"
     gene            6422..7513
                     /locus_tag="PLS88_07270"
     CDS             6422..7513
                     /locus_tag="PLS88_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07181.1"
                     /translation="MRRRYYGLILGIAAIVFSLFSVRSTLPEKEITSIDFLAFSEALN
                     LSIIPMNKSDKILPFVGDSLLGYADLPENKLYVHPFKGRAAIDKAGWITYDRLATNLE
                     LYEKNGREQPLQAYAYPWFKASWRVLLRADQMGIALIDKTGFILWEKEFQMPVTAIDA
                     SSSFLAIGTLDGSISVFDRNGWAILSIQASLQESQTIYGLALSGDSKYLIILRGISPK
                     KIETYRFEDNSFAKLFESSLMVNTTFQATMAIAQDNSHAIMSFGDLLIYYNIKNKYYR
                     ILEAEMLGGDLSENQTDVRYFVLGSLGDSLVAILRSSLEDIASSDVLILNHGLLVHTA
                     HGALGAIGEEEVIGFVYKDGVELLKGWQP"
     gene            7510..>8481
                     /locus_tag="PLS88_07275"
     CDS             7510..>8481
                     /locus_tag="PLS88_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07182.1"
                     /translation="MKGKYCFLMAYLWLTTLLGALDFQIPKVLDISGFGTILADQVRL
                     GISGEYNGKGRFYGMIPRSDVQLISADKTLEAPSSGTVFYSQVEANLPTVFSFPLGGA
                     LAVIHPDKFVSLTSGLDSFQVIESSFRSSKEMQKSSAIKKGEKLSGGVGSGVYTSKSF
                     GLRLFDVKNELWMNTIFLAPWIEDNTSPIIRSARLIKAGTDNKILFDIIEANKKERRI
                     TCPQGAYSLFVDARDIIISNSHFYSAPYRIEAILDGEIVINASFMAARASGGGISFLG
                     NPPPSQRAIGEDCSYQTGQFIILRGKHDFLLQVNDFAGNQTDMRISIT"
BASE COUNT         2461 a         1806 c         1995 g         2219 t
ORIGIN      
        1 taccagagat tggcaggaca acttttgcgg actcgccatt catatttgca gctagaagac
       61 cctgaattct gtcccgtata tgcctttcca atattctctg aaacaagcgt gaaagaaatt
      121 atagattatg ctatgaagca tggtgtagag agcgaatatg cgtttgcttc ttctatagca
      181 aagagaatat ccggcagcga gcctcaagaa gaagtgatat ttcctacagc aagatctata
      241 gcaatgcgct gtattctatt tcctatgcat cataaactca ccaaccagca agtagatcaa
      301 ataggcaaag taattgcaac actaccatag gagattttta tgtcggaaag caaatctacg
      361 ctcattttcg caaatctcaa caaagatgat gctggacatg cagcacaaat tgtgcaagct
      421 gagctagaaa agaaaggctg gcatgcagaa atcctcgcat ttcgaggatc tatatcagaa
      481 tatccagatt ttgatcgata tagccttatc ataagtcttg gtggcgatgg cacgcttctt
      541 tatgccgcta gatttgctgg tcctcgcagt ataccaatac ttccaataaa cttaggaact
      601 ctaggattta tcgccgctaa caagatggat acctgggctc ttactttcga gcattggcag
      661 agcggagatt taaagccatc aaagcgctta atgctgcaat cttgtgcata cagaggaaag
      721 aatattttgc acagaggaat cgcgctcaat gatatagttg tatcgtctga gagcggcgct
      781 agaatgatac gcatgtgttt atcaataaat agagaatgct ttggttatta cagggccgat
      841 gggcttattg tttccacacc taccgggtct actgcttaca atctagcggc gggtgggcca
      901 gccttacatc cagaaatcag tgctcttata ataaatccaa tatgcccgtt tacattagca
      961 tcgaggcctt tagttatacc agcctccaat tacatagata tcgagatcga cgagactaaa
     1021 aaagcagggg ctcttttgat tgcggatggt catatagtgg caaatctcga gaagggcgat
     1081 gtagtccaga ttcgagaata tgagaagagc gcatttttgg ttgttccttc aggaaatgcc
     1141 tttttttctg ctctaaggac aaaactgggt tggtcgggag acaacgatgc ttgagcagct
     1201 ttctgtgcgc aatttcgcca ttattgagag catagacata gatttttcgc ctggaatgac
     1261 agtgttctca ggagagaccg gaactggcaa atctttgatt gttgatgctc ttggattttt
     1321 gcttggcgca cgagtcgaca attcaataat ccgcgaagga gcaacagaat gcgctgtatc
     1381 aggacttttc tcagcttcaa gcgattctca ggttgggaac tggcttaaag agaatggcat
     1441 agaatggcag cctaatgaag aaatcttgct tcgccgagtt ctcaaacaaa acggtcggac
     1501 tctagcatgg atccaagatc gacaagtttc taggaatgag ctcctggaat tcactcaaaa
     1561 tcttgtcgat atacatggtc aacacgaaca tcaaaggtta atagattcat ctactcatct
     1621 ggacatgctg gatgcctatg ctggtctaga agatgaattg cgatcatatc aaaagctcta
     1681 tttggaatgg aaagaagcgg ctcgtcaact gcaggaacta ttggaacaaa aagcaaagcg
     1741 cgcgcaggaa gtagattacc ttgattttgt aattagagac attgcatcga taaagccaaa
     1801 gcccaatgag gacaatgagc ttttggctga ggaaaagata ctatcggagc acgaaaaact
     1861 atttggagca gtttctgagg ccttaatgtt tttagatagc gatgaaagcg ccgatgtgct
     1921 ccatgcctta aaaaaggttc gagctaatat cgaaactgcg cgtaccatag acatccggtt
     1981 ggggtctttt tccgaaagac tttctaccgc ctactatgag ctagaagatg tatccgagtc
     2041 tttgaatgag tataaggcta gccttcgctt cgatcccgat cgtctcgaaa gcattgaaag
     2101 gaggctcgct gatcttcaac gcctaaagca taaatacggg ccaaaacttg atgatgtgct
     2161 acaggcctac gaacgagccc ggagacgact tgaatcgctt gaacatgctg aggagaatgc
     2221 cgaagaactt gaaaagaaag aaaaaacgct cgagaaagag gtatttaata gagcatatca
     2281 gctttctgaa aagagaatga tagccgcggt ttcgatgtct tcttcgataa aagagatagt
     2341 taaaaatcta ggaatgccag aagcgcaggt gaaccttcgt ttgaagagaa tcatagacga
     2401 aagaggagaa tataggacaa cacctcgtgg tatcgatgaa gcggaattct tcattgcccc
     2461 gaacccagga gaacctgaac ttcccttgtc tagaatcgct tcaggaggag agctatcgag
     2521 atttgctctt gcaataaagg ccgtgttggc ttctaaggat gaggttgaga ccttagtgtt
     2581 cgatgaaatc gattcaggga ttggagggca agttggtgtc gcagttggcg aatacttaaa
     2641 gcagttaagc cagtatcgtc aggttttatg cgttacacat ctcgcaacca tcgcagtcca
     2701 agccgaccac caaggcaaag tcgaaaaatc cgtatcaaaa ggcagaacag gtgcctccat
     2761 acgcttcctt tctgggaaag agagagaaac tgagattgca cggatgctat ctggctcctc
     2821 ggagagcgaa gtatccagaa tccacgctgc cgaactgctg aaaaaagcca tttctcatga
     2881 ttaaatagcc taaagcctct tctacttata cttcttttaa cactggcggt taatgagaaa
     2941 aacgtcgatt attatcttat ggcagagaga ggcttaagag cgcctcatag tcaatcaact
     3001 tatgttaaaa ggaattatct tctttacttt attaaaacaa ttgtaaaagc ctagcaaggg
     3061 atattgcatt tattggatta tctttatata aatatatcta tataatttaa ttggatttat
     3121 aaaggagcat ttaaatggca cggtatatca tcgtaggcgg cgtcgcggga ggagcatcga
     3181 cagcagccag gctccgcagg ctcaacgagc atgcggaaat tgtcttattc gagcgtggtc
     3241 catacataag ttacgccaac tgcgggcttc cctattacgc aggagaaacg ataaaagagc
     3301 gagagcgact ctttgtcatg acacccgaaa aattcaacgc atggctcaat gtcgatgtgc
     3361 gtattcgtac agaggtgatc agcatcgacc gcgaggcgaa gaagattcgg gccagagaac
     3421 tggacagcgg ccgcgaatac tcgcttgagt acaatgcgct cgtgctatcg cccggagcag
     3481 agccgattaa accgcccatc ccaggcatcg aagacccacg cattttcacg cttcgatccg
     3541 tgagcgacat cgatcacata aaagaatacc ttgacacaaa gcgtccagag cggatcattg
     3601 tggttggcgg tggtttcatc ggcctcgaaa tggccgagaa cctgcacgca cgaggttcgt
     3661 tcgtcacaat cgtcgaagcc ctcgatcagg tgatgaatcc aatagatttc gagatggcgg
     3721 cgcttgtcca tcagcatctg aaacaaaaaa acgtcgagct ctaccttagt tcagcggttt
     3781 tgaaattcga agaggctggc tcacggattg tcgcggtcct agccgatgga acccgtctcg
     3841 acgccgacat gatcgtgctt tctataggcg tccgccctga aactgccatc gctaaagcag
     3901 ctggactcga aacaacgcca aatggcgcga ttctcgtcaa tgaaaattta cagacgagcg
     3961 atcctgctat ctatgctcta ggcgatgcaa tcgccttccc tcatcctgtg ctagacatgg
     4021 caatgcctgt accgcttgcg ggacctgcta ataaacaggc gcgcgtagtc gcagacaata
     4081 ttgccaaggg ccctggaaca cgaaaatggc atggcgcgat aggcacatcg atcgctaagg
     4141 tcttcgacat caccgtggct gccgcagggg tcgctgaaaa actcctcagg aggaacaata
     4201 tcccctgcat atcgataatt actcatggtt caagccatgc cagctattac cctggtgccc
     4261 agccacttac tatcaagact atattcacac ccgacgggac actccttgga gctcaggtcg
     4321 tcggttacga tggcgtcgac aagcgaatcg acctgatcgc cgattatata cgtcgcaagg
     4381 caaaagtgac ggaacttggc gaaattgagc atgcttacgc accacctttc tcgagcgcta
     4441 aagatcctgt caacattgct ggaatggtgg cggaaaacgt gcttgcaggc ttaagccggc
     4501 atttgcagtg gcatgaggtg aaagccttcc aggaaaaggg tggttttgtg ctcgatgtgc
     4561 gcacccctga agaattctcc atcggcgcca tccctggcgc gaaaaacatt cctctggatt
     4621 cactgcgcca aaaccttggt gaaattcctc agaaccgaga agtgcttata tattgcggag
     4681 tgggtcttcg ggggtatctc gcggagcgaa ttctcagaca aaatggctgg acatcaatag
     4741 ctaacttgtc cggcggctat aaaacatggg agattgcgac cgagcctcaa tctaacaaag
     4801 gtatctacaa gccaggtttc gtgggtttcc agtccggtct gggcaagggc tctcaggaac
     4861 ttctgcacac gacctttgcg gaaggatcgg gcatgcctga atcgttcgca gcgaagagcc
     4921 agacgattgt ccaggttgac gcctgcggtc tgcagtgccc cggccctatc atgcggctca
     4981 agaccgaaat cgacaaacta cctgagggcg gtcgcgtggt catacgttcg actgatccag
     5041 gctttgtccg tgacgcagga gcatggtgca aggtgacagg aaatctcctc atatcgatgg
     5101 aagaatcaaa tggcacatac accgcgatgg tagagaaaac ggccaaacaa agtgccatgg
     5161 ccgttgcgca ggaaacagaa ggcatgagag gagaaccatt cgtacagatg acacccaaag
     5221 gtgcgaccat catcgtgttt tcgaacgatt tcgatcgcgc tcttgcgagc ttcgtgctcg
     5281 caaacggggc agctgctgcc ggcaaggatg taaccatgtt ctttacattc tggggtcttt
     5341 cggtcattcg gaagcccaat gcgccaaggg tcgccaaaga cttcatggga aggatgttcg
     5401 gcatgatgct ccctaagcat gctggtggac tttccctttc ccatatgaat tttggcggaa
     5461 tcggacccaa gttgatgaag tcgcggatga aaagcaaaaa tatcgacatg cttgaaacca
     5521 tgatgtcaca ggcgcgccag gcgggcgttc acttcattgc atgccagatg tcgatggaca
     5581 taatgggggt caaacacgaa gaactgctcg atggagttga ggtcggcggc gtcgcgacct
     5641 acatggaagc tgcgaccgaa ggcaatgtaa atctgttcat ctaaatgcag ctttagaaat
     5701 gtatctggga aacatgtaat actaaactaa agaggaaaga ccttataaca tgtttcattc
     5761 tagctcataa gccatttcgg ttaggaaaaa cgtcgctttt ggcagtgctg attagaaagg
     5821 catagagata ctcgagctca acattaagaa ggatcatccg catacatttg tggtagtttt
     5881 ttattagttt ttagaaactt tcagatcttg ctcctggctg gcaagctcgt gtagggcatc
     5941 gtatggtaaa agttcgccta ctgttgaact tatatatgaa tcttcgctat tgccgaaaat
     6001 taccgtggcg gagggaggca tgaactcaga aatcacttgc cgacatgctc cgcaagggct
     6061 aacggggtaa tctgcatctg gtgttgctat gactagtgct ttgaattttt tcttgccagc
     6121 agcgatggct gcagctattg catttcgctc tgcacagatt gtcaagccaa agcttcggtt
     6181 ttcgacattt acaccagtaa tgatgctatc atcttcgcag agcaaggccg ccccaactct
     6241 aaatttagaa taaggcgcat aactttgttc acaggctttg agagcttgtt ctaataaaga
     6301 agcatattcc gttttcatgt cagttcaaat ccttttctta tactatatcc tgaaatcaaa
     6361 ggaattgaaa tgcgccatat ggtattgact caaagacttc agtcatgcac cataaaatac
     6421 gatgagaagg cgatattatg gccttattct tggtatagct gcaattgttt tttcactctt
     6481 ttcagtacga agtactttgc cagaaaaaga gattacatcc atcgatttcc ttgctttttc
     6541 cgaagctctg aacttgtcaa tcataccaat gaacaagagc gataagattc tgccctttgt
     6601 tggagactct ctcttgggct atgccgacct acccgaaaat aagctctatg ttcatccttt
     6661 caaaggaaga gcagcaatag acaaggccgg atggatcaca tacgatcgcc ttgccaccaa
     6721 tctagaatta tatgaaaaaa atggtaggga gcagcccctt caagcatatg cttatccatg
     6781 gtttaaggct tcgtggagag tactgcttcg ggctgaccaa atgggtatag cgctaataga
     6841 taaaacagga tttatcttgt gggaaaagga attccagatg ccagtcacgg caatcgatgc
     6901 atcttcttcg ttcctcgcta ttggaacact tgatggatcg ataagtgtat ttgatcgaaa
     6961 tggatgggcc atcctttcga tacaagctag ccttcaagag tctcaaacaa tctatggtct
     7021 agctttatct ggagacagca aatacctcat aattcttaga ggtatttcac cgaaaaaaat
     7081 tgaaacctac aggtttgaag acaattcatt tgcgaagcta ttcgaatctt cattgatggt
     7141 gaatacaact ttccaagcta ccatggcaat agctcaggat aattcacatg caataatgag
     7201 ctttggtgat ctgttaatat attataatat aaaaaataaa tactatagaa ttcttgaagc
     7261 agaaatgcta ggaggagatc tatcagaaaa tcagactgat gttaggtatt tcgtgcttgg
     7321 ttcgttagga gactcgcttg ttgcgattct gagaagcagt ttagaagata tagcaagctc
     7381 tgatgttctc attcttaatc atggtcttct agtacatact gcacatggag ctcttggagc
     7441 aattggcgaa gaagaagtaa tcggtttcgt atataaagat ggcgttgagt tgctaaaggg
     7501 atggcagcca tgaaggggaa atattgtttt ttgatggctt atttatggct tacaacatta
     7561 cttggcgcat tggactttca gatacctaaa gttcttgata tatcaggctt tgggacaata
     7621 cttgccgatc aagtaagatt aggaatatcc ggagaataca atggaaaagg aagattctat
     7681 ggcatgattc caagaagcga tgtacagttg atatcagcag ataaaacact tgaagcacct
     7741 tcaagcggta ctgtttttta ttcacaagtt gaagctaatt taccgacagt cttttccttt
     7801 ccattaggag gagccttggc tgttattcat cccgataaat ttgtgagtct aacttcaggg
     7861 ctagattctt ttcaggtcat agaatcatct tttagatcat cgaaagaaat gcagaagagt
     7921 tcagctatta aaaaaggaga aaagctctct ggaggagttg gttcgggtgt atatacatcc
     7981 aagagttttg gtttgcggct tttcgatgta aagaatgaac tatggatgaa tacgatattt
     8041 cttgctcctt ggatcgagga caatacatct cctataattc gtagcgcaag actgattaag
     8101 gcaggcacag ataataagat attatttgat ataatagaag caaataaaaa agaaagacga
     8161 attacatgtc ctcaaggtgc ttattcattg tttgtcgatg cacgagatat catcatatcc
     8221 aattcacatt tctattctgc tccctatcgc atagaggcca tccttgatgg ggaaattgtc
     8281 attaatgctt catttatggc tgcacgcgca agtggagggg gaatatcatt tctgggtaat
     8341 ccacccccat cacaaagagc gataggagaa gattgttcct accaaactgg tcaattcatt
     8401 attctcagag gtaaacacga ttttttgctt caagtaaacg attttgcagg aaatcaaact
     8461 gatatgagaa tctccataac c
//
LOCUS       DAOSOB010000068         8442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_8355_length_8442_cov_79.547872, whole genome shotgun sequence.
ACCESSION   DAOSOB010000068 DAOSOB010000000
VERSION     DAOSOB010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8355_length_8442_cov_79.547872"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(423..1391)
                     /locus_tag="PLS88_07280"
     CDS             complement(423..1391)
                     /locus_tag="PLS88_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU07183.1"
                     /translation="MNPRQPSRHIPGIPDLSEHDFKKTKEIESSDMKGGISIEKSWTG
                     MNERKSLSPNPIASSSQEMPKTILEANNLSIGWKKGRKFNVLAQDINFKAKEGYLIAL
                     VGPNGAGKSTLLKTIAGLQPPCGGSLFLMGKEISRISVEERAQLLACVFPERIETGYL
                     TVWELVAFGRYPYTNARHRLTKEDRQQVMEALSMVGMENFKNRTVSSLSDGERQKVQI
                     ARAVAQSTALLIFDEPTAFLDAPSRIEVFRLAERLVRQAGKTLLLCTHEVDLALKTAD
                     ELWVIDRAHHFSAGAPFVIARSGAIGRAFDLPSVAFDVFSGTFKAR"
     gene            complement(1388..2536)
                     /locus_tag="PLS88_07285"
     CDS             complement(1388..2536)
                     /locus_tag="PLS88_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU07184.1"
                     /translation="MNENEKNKNRNHEKMTENQPKINKEPRQRLLVLYLAGAVLLLAL
                     LDIALGSVCIPLLEVLKSLAGKASRPEWTTIVLVFRLPKMLTAAMAGAGLSVAGLILQ
                     SIFRNPLAAPDSLGIGAGASIGVAVLMFAGESLGALGAGAKGIGTQGSALLGNLPPLG
                     YTLLVIAASAGAAAVLIIILLISKRFEQAVTVLIMGLLVGYLASSLVSLLVYFGSPQK
                     VQLYLSWTYGSFGGVRTNELPYLAIAIGIGFIIALGEIKALNAFLVSERFASTIGIRI
                     RASRTKLLVAASILAGSITAFCGPIAFLGIAAPHVARLLSRKADHRILLPIAALEGAC
                     FALLADIISSLPTYRSLLPINPILALIGSPIIISMFFHRNKGSMEAVE"
     gene            complement(2554..3723)
                     /locus_tag="PLS88_07290"
     CDS             complement(2554..3723)
                     /locus_tag="PLS88_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU07185.1"
                     /translation="MRRIAGIFLSLFLVFFPLFGLQRTYSQQASAQSTSSQQATSLQT
                     SAQHASFSIEQKNGYKLLKAYNLWPGASSTKTYVLYPRGSKVPRDVSADLAIAVPVRR
                     VVLYSTTYIPAIEAIGELDAVVGVDNAAYIYNSALRTQIATGKTIETSKNWMPDIERL
                     IMLKPDIIFNYGIGNEWDTFPKMQEAGLPVVLIGDWNEQDPIARAQWAVFIAAFFDKE
                     NIALERLDAITKAYESLRSLALGASKRPKVLVNGPFQGIWSVSGGQSYMARLIKDAGG
                     DYLWADNKDTGALNLSVEAVFERALKADVWLNPVSGAKQLSDVRAMDPRFSALPVLQK
                     GQVWNNDLRCSPEGGNDYFESAVMNPHLVLSDLIAIFHPELMPNYKFNYYKKLNE"
     regulatory      complement(3749..4161)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4233..>5105)
                     /gene="proB"
                     /locus_tag="PLS88_07295"
     CDS             complement(4233..>5105)
                     /gene="proB"
                     /locus_tag="PLS88_07295"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOU07186.1"
                     /translation="CAAIGQPILMEEYRRAFGVFGLVAAQLLVTRDEWDDRASYLNLR
                     KTVETLLETRAIPVFNENDSVSTAEIGNAFGDNDRLSAYVASKIDAELLIILSDVDSL
                     YDSDPRENPNAKPIPYVRELSEEHLSAAGGRGSEFSTGGMKTKLAAVSIARDAGCRVV
                     IAHGREPDVIKRIAHGEALGTLFDATHALKNRIRWLKNSQPRGRLTIDEGALAAIKGH
                     NSLLPAGVVAVEGDFGRDSVVLVNGIIKLISNFASTELRAIMHKRSEEIDLILGDNAP
                     HVVARPEEMAFLDE"
     assembly_gap    5106..5205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5359..6669)
                     /locus_tag="PLS88_07300"
     CDS             complement(5359..6669)
                     /locus_tag="PLS88_07300"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOU07187.1"
                     /translation="MQQRLERASLAASRMLSLETKVKNSALAGIAQSLRENRDSIFYA
                     NQLDLTAASQNGLAAPLFKRLEFDEKKLADVLAGIQDILVMPDPVGKVLEARLLDEGL
                     ILKRVSCPIGLIAMIFESRPDALVQMASLAVKSGNAIVLKGGSEAKESNKALATVIAE
                     AGEEMGLPSNWLTLLETREEVSELLAFDKYVDLVIPRGSKEFVAKIKAESRIPVLGHS
                     DGVCHVYVHEDADPVMAIKIALDSKTQYPAACNAAEVLLVHAGFAHRSLMPLLRALSK
                     AGVMLDVCENVAQMLDKDSQQTQSLHYSVKTDTDWTVEYLGMRMAVKIVDSLDEAIAH
                     INHYGSGHTDVIVCSSSEAAERFLKEVDSASVYHNASTRFADGYRYGLGAEVGISTSK
                     IHARGPMGLEGLCTYKWLLEGQGHIVSDYASGVRHFVHRDLPVD"
     gene            6808..7686
                     /locus_tag="PLS88_07305"
     CDS             6808..7686
                     /locus_tag="PLS88_07305"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU07188.1"
                     /translation="MAQRTIGFIGLGVMGAPMAGHIRASGERLLIYTRTKSKARLVLD
                     TGAEWRDSPKQIVEECDVVFTMVGYPKDVEEIYFGPDGLIENARPGTIFVDFTTSSPD
                     LAVKIYESAKARGLSAIDAPVSGGDIGAKNATLTIMVGGDEETFQKVKPLLETVGKTV
                     ILQGGPGAGQHTKMANQIAVAGNLLGTVEALIYAKNAGLDPKHVLLSIANGAAQSWQL
                     SNNAPRMLERNFDPGFYIKHFLKDLRIALDSAHAMHIELPMLALSEKLFAKMVNEGMG
                     ELGTQAIYLLYERGLA"
     gene            7841..>8442
                     /locus_tag="PLS88_07310"
     CDS             7841..>8442
                     /locus_tag="PLS88_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU07189.1"
                     /translation="MSIEESGASNSLAREASFWRHIYETQQAGILIQVNGIINDLNET
                     AIQMLGGQSASEFIGKPSIETLICPEYIDCILDRTRQLRNGRPSVPPIEIRVRQLNGA
                     EVDVLISATSWIENGDTIIETMLIDIGSLKQRERLLQAISDLASMPVKKEFFSDTSLA
                     MSYVFSSMKKLYVEPHNCGFISFVYHENGFLEEACESFSS"
BASE COUNT         2199 a         1952 c         1995 g         2196 t
ORIGIN      
        1 ccaatcctgt cgaggttgta agggctatta tagatcaagg agccgcaatc ctcggcgtgg
       61 tcgatggttt ttcgcctaaa ggcgtagaga gtgcagaaga taaggttgcc cgaaaagcca
      121 tgttgcgaga gtttgggtat aaattctaag ttttcgacag gattgacggg attttttgga
      181 ctggatgaca ggatgagatg aggattgagg gggttttttc gacaggattg acatgatgag
      241 atgaggattg atgggatttt tggactggat tgacgggatt ttttggactg gatgacagga
      301 tgagatgagg atttacagga taagaatgtt tttagggaaa aagagaagcg ctatcttttc
      361 tttatctaaa ataaaatcct atccatcctg cctaagctca gcaccattca ttataaaacg
      421 agctatcgtg ccttgaatgt gccgctaaag acatcgaaag caacggacgg cagatcaaag
      481 gctcttccta ttgcccctga acgcgctata acaaagggag ctccagcaga aaaatgatgt
      541 gcgcggtcga tgacccagag ctcatccgct gtcttaaggg caagatccac ttcatgcgta
      601 cagaggagaa gagtcttgcc tgcctgccgt acaaggcgtt cagcaagcct gaaaacttca
      661 atgcgagaag gagcatccag aaaagcagtc ggctcgtcga agatcagtag cgccgtgctc
      721 tgcgccactg cccgggctat ctgcactttc tgtcgctctc catctgagag gctggacaca
      781 gttcggttct taaaattttc cattccgacc atcgaaagcg cttccattac ttgctgtcta
      841 tcctcttttg tcaagcgatg gcgtgcattc gtatagggat atcggccaaa ggctaccagt
      901 tcccacacgg tgaggtaacc agtttcgata cgctcaggga aaacacaggc gaggagctgt
      961 gctcgctctt caactgaaat cctcgaaatc tcttttccca tcaagaatag agagccgccg
     1021 cagggaggct gaagccctgc tatagttttc aacagtgtgg atttacctgc tccattcggg
     1081 ccaacaagag cgatcagata cccctctttt gccttgaaat tgatatcttg tgctaggaca
     1141 ttgaattttc tgcctttctt ccagccaata gacaagttat tggcttctag aatcgtctta
     1201 ggcatctctt gggaagaaga agcgatagga ttagggctga gacttttcct ctcattcata
     1261 cctgtccaag atttttctat cgatattcct cctttcatat cagaactttc gatttcttta
     1321 gttttcttga aatcgtgttc tgataaatcg ggaataccag gaatatggcg tgaaggttgc
     1381 cttggattca ttctacagcc tccattgagc ctttattcct gtggaaaaac atcgaaatga
     1441 taattggaga acctatcaag gctaggattg ggtttatagg caatagcgat ctataagtag
     1501 gaagggatga tattatgtca gccagcaaag cgaaacaagc tccctcaagc gcggcgatcg
     1561 gcagaagaat tcgatgatcc gccttacgcg atagtaggcg agcaacatga ggagcggcga
     1621 ttcctaaaaa tgctatgggt ccacagaaag ctgttatgct tccagctagg atcgaagcag
     1681 ccactaaaag ctttgttctc gaagctcgaa tcctaatgcc tattgtagaa gcaaagcgct
     1741 cgcttactag aaaggcgttg agcgctttta tctctcccaa ggcgatgatg aatccgatac
     1801 ctatcgcaat tgcgaggtat ggcagctcat tcgtgcgtac gcctccaaat gagccatagg
     1861 tccatgaaag atatagctgg actttctgag gagaaccaaa atatacaagc agcgatacga
     1921 ggctagatgc aagataacca actagcagac ccattataag caccgtcact gcctgctcga
     1981 atcttttcga tattagaaga ataatgatta gcacagccgc tgccccagcg ctagccgcga
     2041 ttacaaggag cgtatatcca aggggcggca gatttcccaa gagtgcgctc ccttgagttc
     2101 ctattccctt tgcaccagct ccgagagcac caagggattc tcctgcaaac atcaatactg
     2161 ctactccaat agaagcgcca gccccaatgc cgagagagtc cggtgctgcc aaaggattgc
     2221 gaaaaataga ctgtagaatg agccccgcaa ccgaaagccc cgctcccgcc atggcggccg
     2281 ttagcatctt aggtaatcga aatacaagga caatggtagt ccattctggt cgcgatgcct
     2341 tgccggctag cgacttcagc acttctaaga gaggaatgca taccgaaccc aatgctatgt
     2401 ccagaagagc aaggagtaga acagcaccag caaggtatag gacgagtagc ctttgtcgtg
     2461 gttctttgtt gattttgggc tgattctctg tcatcttttc atgattccta ttcttgttct
     2521 tctcattttc attcatgcgg ctgcgcttag gctttattcg tttaattttt tataataatt
     2581 aaatttatag tttggcataa gctccggatg aaagatcgct atcaagtcag aaagaacaag
     2641 atgaggattc attaccgccg actcgaagta atcgttgcct ccttctggag aacatctgag
     2701 atcgttgttc caaacctgcc ctttctgtag aactggaagt gccgagaagc gagggtccat
     2761 agctcgcaca tcggaaagtt gctttgctcc tgagacggga ttgagccaaa catctgcctt
     2821 tagggctctt tcgaaaaccg cttctaccga aagattgaga gctcccgtgt ctttgttatc
     2881 tgcccaaagg taatcaccgc ctgcatcctt tattagcctt gccatatagc tctgtccgcc
     2941 cgatacgctc catatccctt gaaaaggacc atttacaagc acctttggcc gcttcgaagc
     3001 gcctaaagcg agggatctga ggctctcgta agcttttgtt atagcatcca agcgctctag
     3061 ggctatgttc tccttatcaa agaaagcagc gatgaataca gcccattggg ctcttgctat
     3121 tggatcctgc tcgttccaat ctccgatgag cacgacaggt agccccgcct cttgcatctt
     3181 tggaaaagta tcccattcat tgccaatgcc ataattgaat atgatatcgg gctttagcat
     3241 aatcagccgt tctatatcgg gcatccagtt tttagatgtc tcgatagtct tacctgtggc
     3301 tatctgtgtg cgtagggcgg aattatatat ataagcagcg ttgtcgacgc ccaccacggc
     3361 atctagttct ccaatcgctt cgatggctgg aatataggtt gtagaataca gaacaactcg
     3421 ccgtacaggt acagctatag caaggtctgc agagacatct ctcggaactt tggatcctct
     3481 agggtagagc acataggttt ttgtcgaaga ggctcctggc cagagattgt aggcctttag
     3541 tagcttgtag ccattttttt gctctatgct gaagcttgca tgttgagcgc tggtttgcag
     3601 agatgtggct tgttgagagc ttgttgattg ggcgcttgct tgttgcgagt atgttcgctg
     3661 caggccgaac aatggaaaaa agacaagaaa caaggaaagg aaaataccgg cgattcgtcg
     3721 catatccgcc tcctcccacc gaagagcaag gatgaaaatt ctcaagggca ggtttcctgg
     3781 cttgctggcc atgcaattct agacattcta caattcacaa atatctacgt gagaaaagct
     3841 agaaatgcat atgatgcggt cttccttccc agccccgatg gcttttgttc ttcttttatc
     3901 tatatcttct cgattggaag cctatcgact agaagccgaa caaaagcatt aagaccagtg
     3961 gatcagccaa ggctaacaaa acgtgcaatt atcatgatag cgctcttagc cttgactgca
     4021 gcgacattgc acaatatcga tatcgctgga ctgcagctat accagctcac agtagcgggg
     4081 gctgcaacgg ttttccaccg tcttcccttt tccgctgcac ggcgacgccg cacatacggc
     4141 acccatgagc ttaggttatt ttactacaat gggcgaaatg agaaaaggcc gaaacttggc
     4201 gctatgtgct ctatcagaaa aacatgaaaa aattactcat ccaaaaaagc catctcttcg
     4261 ggtcttgcga ctacatgggg cgcattatcg ccaaggataa ggtctatttc ttcgctcctt
     4321 ttatgcataa tcgctcttag ttccgtcgaa gcaaaattgc ttatcagctt tatgattccg
     4381 tttaccaaga cgacggaatc tcggccaaaa tcgccttcta ccgcaacaac accagctggt
     4441 aaaagcgaat tgtgcccttt tatggcagcc agcgcacctt catctatcgt aagcctgcca
     4501 cgaggttgag aattcttgag ccatcgtatt ctatttttaa gcgcatgggt cgcatcgaaa
     4561 agcgttccaa gagcttcccc gtgggcaata cgcttaatga catcgggctc acggccatgg
     4621 gcgatgacca cgcggcaacc cgcatcgcgc gctatagaaa cggctgcaag ctttgtcttc
     4681 attcctcccg tggagaattc cgaacctcga ccaccggctg ccgaaagatg ctcttccgat
     4741 agttctcgta cataggggat gggcttggca ttgggattct cccttggatc tgaatcatag
     4801 agcgaatcta catcggaaag aatgatgagc aattcggcat cgatcttgct cgctacatag
     4861 gccgatagcc tgtcgttgtc accaaaggca ttgccaatct ctgccgttga gaccgaatca
     4921 ttttcgttga agacagggat cgctcgggtt tccaggagag tctccacggt ctttcggaga
     4981 tttaggtaac ttgcgcgatc atcccactca tctcgagtca caagtagctg agctgctact
     5041 aggccaaaga caccaaacgc tcgcctgtat tcttccataa ggatgggctg accgattgcc
     5101 gcacannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntctgt aaagccgagt
     5221 gagacggact gggctcgatt caggtctgga aatggtcgct gtaccgattt tgactacaat
     5281 gcggtttgcg tgcgcaagtg ctgctcgagc agaagcttca tccatagttt catgagtttg
     5341 catagctctt ttcctctctc aatccacggg aagatctcta tggacaaagt ggcgtacgcc
     5401 cgaagcatag tcactcacga tatgcccctg gccttcgagt agccatttgt aggtgcagag
     5461 tccttcgagt cccattggac ctcgtgcgtg gatcttgcta gtcgagatac cgacttctgc
     5521 acccaggcca tagcgatatc catctgcaaa tcgagtgctc gcattgtggt agacagatgc
     5581 tgaatccacc tccttaagga agcgctctgc tgcttcggaa gaagaacaga ctattacatc
     5641 ggtatgacca cttccataat ggtttatgtg tgcaattgct tcatcgaggg aatcgacaat
     5701 cttgacagcc attcgcattc cgagatattc gacagtccag tcggtatcgg tcttgacgga
     5761 ataatgcaag ctctgggttt gctggctgtc tttatcgagc atctgggcta cattttcgca
     5821 gacatcgagc atcactccag ccttagaaag agctcgcaaa agcggcataa ggctacgatg
     5881 ggcaaatcca gcatgcacga gaagcacctc cgctgcattg caggccgctg gatactgcgt
     5941 cttcgagtcc aaggcgattt tgatagccat taccgggtct gcatcttcgt gcacatagac
     6001 atggcataca ccatccgaat gtccaagcac aggtatacgg ctttccgcct ttatctttgc
     6061 gacgaattcc ttggaaccac gaggaatgac tagatcgaca tatttatcga acgccagcag
     6121 ttcgcttact tcttcccgtg tttccaggag agtgagccag ttcgaaggga gtcccatttc
     6181 ttcgccagcc tcggcaatta ccgtggccag agccttattg ctctcttttg cttcgctgcc
     6241 tcctttgagc acgatggcgt ttccgctttt aaccgccaat gaagccatct gcacaagggc
     6301 atccgggcga ctttcgaaga tcattgcgat gagcccaata gggcagctga cccttttcaa
     6361 tattaggcct tcatccaaaa ggcgcgcttc tagaaccttt cctacaggat caggcataac
     6421 aaggatatcc tgaataccgg caagcacatc ggccaatttt ttttcgtcga attctaggcg
     6481 tttaaaaaga ggcgctgcca gtccgttttg acttgcagca gtcaggtcca attgatttgc
     6541 gtagaaaatg ctatcgcgat tttctcgcaa ggattgcgcg ataccagcga gcgcagaatt
     6601 cttgaccttt gtttctaagg aaagcatcct cgaagcggca agacttgctc gctcaagtct
     6661 ttgctgcata ttcatgcata atactatata aaaaattaat gaaatcaaaa agagggtgaa
     6721 atgtagtata tttgaaaaga tgaaccacgc ggtttgaaat aattacccgc ggaaactgca
     6781 aatccaaatt gcaaaggaga ctgtgaaatg gcgcagagaa caattgggtt tattggacta
     6841 ggtgtgatgg gcgctcctat ggccggacat atacgcgctt cgggcgaaag gcttttaatc
     6901 tatacacgca caaaaagtaa ggcacggctt gtattggata caggagccga atggcgagat
     6961 tctcctaagc agattgttga ggaatgcgat gtggtcttta cgatggttgg ctatccaaaa
     7021 gatgtggaag agatatactt cggccccgat ggccttatag agaatgctag acctgggact
     7081 attttcgtcg actttacgac atctagcccc gatcttgctg ttaaaattta cgaatcggca
     7141 aaagctcgcg gactgagcgc aatagatgcg cctgtttcgg gtggcgatat aggcgcaaag
     7201 aatgctactt tgacgattat ggtcggcgga gacgaagaga cattccagaa agtaaagcca
     7261 cttcttgaga cagtaggcaa aactgttatc cttcaaggcg gtcctggcgc tggccagcat
     7321 accaagatgg ccaaccagat cgccgtggca ggtaatcttc ttgggacggt ggaagctctt
     7381 atctatgcaa agaatgcagg cctcgatcca aaacatgtgc tcttatctat tgcgaatggc
     7441 gccgcccaaa gctggcagct ctcgaacaat gctccgcgga tgctcgagag gaatttcgat
     7501 ccaggctttt atatcaagca ttttctcaag gatttgcgca tagctttgga ttctgctcat
     7561 gcaatgcata ttgaactgcc gatgttggct ctttccgaaa agctctttgc caagatggtg
     7621 aacgagggca tgggagaact gggcacgcag gcaatatatc tgctctatga gcgcggatta
     7681 gcataaaaat gcagtcttgc ctttataatc tttttattat gaacgataat agattaagcc
     7741 ttgtgaattt cataccgata cagtaaacat tgccgaagtt atcttctggg ttagagaaaa
     7801 tgattgcctc aatgaggcga aataggtagt ggcataaaaa atgagtatag aagaatctgg
     7861 agcgtctaat tcgcttgcaa gagaggcttc gttttggcgg catatatacg aaactcaaca
     7921 agctggcata ttgattcagg tcaatggaat aattaatgac ttgaatgaga ctgctatcca
     7981 aatgctcggc ggccagtccg catctgaatt cattggaaaa ccttctattg agacattgat
     8041 atgccccgaa tatatcgatt gcattctcga tagaactcgc caactacgaa atggtcgccc
     8101 ttcggttcca cccatagaga tcagagtgcg acagctaaat ggagcggaag tggatgtgct
     8161 aatatcggca acttcatgga ttgaaaacgg cgatacgata atcgaaacta tgctgataga
     8221 tattgggtcg ctaaagcagc gcgaaaggct attgcaggca atttctgact tggcttcgat
     8281 gccggtaaag aaagagtttt tttctgacac aagtcttgct atgtcttatg tttttagctc
     8341 tatgaagaag ctatatgtag aacctcacaa ctgcggtttc ataagttttg tttatcatga
     8401 aaatggcttt ttagaagaag cctgtgaaag cttctcgagc ga
//
LOCUS       DAOSOB010000069         8096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_8858_length_8096_cov_68.335903, whole genome shotgun sequence.
ACCESSION   DAOSOB010000069 DAOSOB010000000
VERSION     DAOSOB010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8858_length_8096_cov_68.335903"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1877)
                     /locus_tag="PLS88_07315"
     CDS             complement(45..1877)
                     /locus_tag="PLS88_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07190.1"
                     /translation="MRLISRKQNFNARYSALIFCIALIFSAANREAYAQETATWNVIK
                     AKSGSQGNLQANSPIHAADLFFPGDDEYDEAFSAAIRQGRVPKTNVFPLFGSELGASL
                     NNDIKESELLSGIVFRASTPTLYYDSPAKQSDLGTSLLEDPSLDINTFPEILDFWQTL
                     PDVLALGLNLYPSASIAFNIDISFNYRRDKIVGQSSPFQWDIDDFRENLTSAAQFPLR
                     TYIAWVGKGYGVALGRFPSGMGWGTLSGSILNPRASSYDQIRFYLESGPFRFTSMTAT
                     SSAQLSKAEQEIQFRTFSDGSNFWDSLNDHDYAAHQMAIKMANWHEIEWKPAPWLELS
                     VAEMSVIGGRTPSLSFVLPTTIWHNTYAAGYSNVGVSLSAAIVPFRGLMLSGELFIDD
                     MKGPRESADAKPQSLAWLGTARWSTLLFDRISLDTGLEYQHVDRWTYMRWSPYLAMYQ
                     RQTLPDGKSGVDQSLGAPWGPDYDSIGAYTNMKFKNGARLGLSYEFIRKGPIYQGMAS
                     KVSVTADFDGDPTTTATTEKIWVPVYYDYDKYAGDGALEAILSRPDEYRHLLSIIGAM
                     PLEKAIELQLSTTIGFYRNFRHVEGASETALLLYAGIVVHLPAK"
     gene            complement(1914..3041)
                     /locus_tag="PLS88_07320"
     CDS             complement(1914..3041)
                     /locus_tag="PLS88_07320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU07191.1"
                     /translation="MINEQLQVSIYHDIDRPAAIWDSLVQSPCIYAFQTRPWLSTWQN
                     TIGKAHGVAPFIIVFKKGQNTALFPLGKKKVRFGALSSTMLIWLGYGVSDYAAPIFSN
                     ETNFDMVELFAHLKNIAKKEHCCAIYLDRIPKFWADGTPLIFAKALLALPFGHRLHYS
                     AHAMQLSQDPLMRLNTKERAHLRRAKRRLSEAGTLSFIIAGDKSLRERLTHTMIAFKQ
                     ERFRQIKVHDNFADPAFRNFYIQASQNDNIPAHICALMLNKNPLAVHWGLSCPPRLYY
                     LMPAFDTENYSSLSTGNLLLVKLLEYCAENDYQLIDFANGDEPYKEKWCDIELPLYEL
                     YIPANLAGWFIALFGSALESIKRSALAPHLKQMAQFLKLSM"
     gene            complement(3038..4618)
                     /locus_tag="PLS88_07325"
     CDS             complement(3038..4618)
                     /locus_tag="PLS88_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07192.1"
                     /translation="MKKLYLPAIAILLFLIFSGIFTLSAQQASQSAAAQQPSQSVFQQ
                     PSEIAEQGLLFTLATELPYRAGTYREEIEARGLTPNASISLQYEQPHIGLIAAIDLTS
                     DQVYGPWLADSPTGALGNFNFMIKEAGIRIFGDSFSLKGGRLRHFDVVDSPYSLFINA
                     NGIAAPMLDFVYRDDRFFYESRYISLTLDSAMHTPAWPDDPSYTGFPDRGANFKVFGL
                     SFGRLTIGFEDSSVYSGRAFDWEYFLSPMPQYFTQYVRGTGGRPWYANYDDNYNAGLF
                     VTWKEPGAYDLYAQAFVDDLGMLGLFGWTNNPWQIALALGGRIKTPKGTFGLHLAGAT
                     KYTFEPGDMRTPTTANQIQSSYGYTYFPETRFEYEWRPSYPKSYKEIAPELNLIGYQY
                     GENNLAMRLDWNGSVSKFECEAFFEFRLSGSNSPANPWHDLWVDPQVAFTWLDDAVLE
                     KRFTISARAITGHSPWQFFAKATGSLALDALELRDPIGVSSSQTLVDQKVQIYSPVPG
                     NTKLLGELTLGVLLRLGI"
     gene            complement(4726..6312)
                     /locus_tag="PLS88_07330"
     CDS             complement(4726..6312)
                     /locus_tag="PLS88_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07193.1"
                     /translation="MKHRCKMRAANSNLFFLYFIFSIFLFSGSRMLFGRNFLFAQTQS
                     RPTSLQWRIVLSINIEYSLRSDNVRIEPPVSQYPSVAYSNGIDIQRNYFGQLPFAVLS
                     IEAGSALDAQGFDPWLCNAQGLGVAAEIDLRHSWAGDYFPVHNLPLNGTTPDVEGNIV
                     ARSVLYWKSPIFEIGFGRDRLDYNGILEGGFLPSRRIPYYDALRAQGKLGCFAFDWLV
                     STIQAVQAWDKNDISPEAGYGFEDQPGTPTIIVEAMNRFSCHLGWVTLGVTDHAMMSR
                     LNNHFVLTDFFPIGSRHQASVAQTNNSMVFDASWEPISGLTIAGQLGLDDIDLNLVGI
                     GDTGSPTISAYIAGVRYSGQIGRVRALPFAIYMEAGSTHWLWGNYDASQAWPSIGDVN
                     YFARFIYRFPRYLGGSILLPLTSPYGPGVTWAQSHGELIIPIGSNNNKNPLSLGVGYR
                     LLFLDMNTDANLITTQVYNNSTTPAAPHWKSFLISLPLRLSAGLFSFDIAPSLVRSDN
                     SMAFELDIALSGTIAFGTVH"
     gene            complement(6382..7275)
                     /locus_tag="PLS88_07335"
     CDS             complement(6382..7275)
                     /locus_tag="PLS88_07335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU07194.1"
                     /translation="MENAMIGSTLRAVAYHAVPKSSESSFSRQLDYFAKHYIPLDAEG
                     FARFLDQGSCEVSGMRRKYGHSAGKAKISNKQASMQCKPGLIISFDDGLMEHYEIAAP
                     LLEARGMRGWFFVTSTLPGLAPKFQEGFCASHEISLHKKTAHLGKAQHSFVSSHVAMS
                     WNEILDLAKRGHVIGCHTAHHVRMRPTLDTAILFDEIVEAKKELEKHLGYPVNAFAWV
                     GGESDTYRADALQLLCDAGFSWIFTTQSSLFRVGEDARAIHRTVLEPDLPFSLFLAKI
                     SGLSDLAHCRRRRASLNAMRL"
     gene            complement(7293..>8096)
                     /locus_tag="PLS88_07340"
     CDS             complement(7293..>8096)
                     /locus_tag="PLS88_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07195.1"
                     /translation="SYKQENDAGSWSYLAIAETTLSLRIPLFSVASASMLAFTPSAGL
                     SLSYDLAKGMVYDAGLSASGSVGYNSIKWKGNFRKGSSASLSESYMFHIGSKHSTRPL
                     DLVLSSQLSAFYSWFNLLGIDFRAIGRWDAGWSLYNDLSYFDDVDASTYLRGIRGPIY
                     GDLAAIVNLQIPVNFAQGKFFQWEAMDAEIFIIPFFDLGYVRTNPANPLWSIDNLLMG
                     GGLDVVIFPEHARSFTYRLSLGYNIEHYLETKTLDWGDTEIWLGIGLHL"
BASE COUNT         2190 a         1807 c         2009 g         2090 t
ORIGIN      
        1 ttttcacaca aattcctctt tgaaagataa gattgaaaga ccagctattt tgcaggcaaa
       61 tgtaccacaa tacctgcgta taaaagaagg gctgtctcac ttgcgccttc aacatgtcgg
      121 aaattacggt aaaaacctat ggtagtagat aactggagtt ctatggcttt ttctagaggc
      181 attgcgccga taatcgataa aagatggcga tactcatcgg gtcttgaaag aatagcttca
      241 agagcgccat ctcctgcata tttgtcgtag tcgtagtaga ctggtaccca gattttttca
      301 gtggtggccg tggtggtggg gtcgccatcg aagtccgcgg taacgctgac tttgctcgcc
      361 attccctggt agatgggtcc ttttcggata aattcgtagc tgaggccaag acgagcgcca
      421 ttcttgaact tcatgtttgt atatgcgcct atgctgtcat aatcgggacc ccagggagcc
      481 cctagggatt gatccacacc cgactttcca tcgggaagcg tttgtctttg atacatagct
      541 agataaggac tccagcgcat ataagtccag cgatcgacat gctggtactc taggcctgta
      601 tctagagaaa tacggtcaaa aagcaatgtc gaccaacgag cagtgccaag ccaagcaagg
      661 gattggggct tggcatctgc agattctcta gggcctttca tatcgtcaat aaagagctct
      721 ccagacagca ttaagcctcg aaatggaact atggctgcgc tcagcgaaac cccaacattg
      781 gagtatcccg ctgcataggt attgtgccag atcgtggtag gaagaacaaa actgagcgag
      841 ggtgttcttc cgccaatcac gctcatttcc gcaaccgaaa gctctaacca aggcgccggc
      901 ttccattcga tctcgtgcca gttggccatc tttattgcca tttggtgcgc tgcataatca
      961 tggtcattga gcgagtccca aaaattgctc ccatcagaaa aggttcggaa ttgaatttcc
     1021 tgctcggctt tggagagctg tgcagaactt gtcgctgtca tgcttgtaaa gcgaaatgga
     1081 ccggattcga gatagaatcg tatttggtcg taagatgaag cccgtggatt taagatggaa
     1141 ccggacaggg ttccccagcc cataccagaa ggaaatctac ccagggctac accatagcct
     1201 ttaccaaccc aagctatata tgttcttagt ggaaattgtg cagcggatgt gaggttttct
     1261 cgaaaatcgt ctatatccca ttggaatggc gaactctgac ctactatttt gtcgcgtcga
     1321 taattgaacg aaatatcgat attgaaggcg attgaagccg aaggatacag gtttaagccc
     1381 aacgccaata catccggcag ggtttgccaa aaatccagga tttctggaaa ggtattgata
     1441 tcgagactgg gatcctctag aaggctcgtg ccgagatcac tctgctttgc aggcgaatcg
     1501 taatagaggg tcggcgtact tgccctgaag acaatgcccg aaagaagctc ggactctttt
     1561 atatcattgt tcaaggaagc cccaagctca ctaccaaaaa gaggaaagac attggttttg
     1621 ggcacacgcc cttggcgtat agcagccgaa aaagcctcgt catattcatc atcgccagga
     1681 aagaaaaggt ctgcagcgtg gatggggctg ttggcttgca aattgccttg cgagcctgat
     1741 tttgctttga taacattcca tgtagccgtc tcttgtgcat aagcctctcg attagcagcg
     1801 ctaaagatca gcgcgatgca gaaaatcagc gcagaatatc tagcattaaa gttctgcttt
     1861 cttgatatta accgcattgt atcccttctc tatcagttta ttccatgatt gtatcacatg
     1921 gataatttta agaattgagc catttgtttt agatggggtg caagagcgct tcgcttaatg
     1981 ctttctaagg cactgccaaa tagcgcaata aaccatccag ctaggttagc cgggatatat
     2041 aattcatata gaggcagctc tatatcgcac catttttctt tatagggttc atcgccgttg
     2101 gcaaagtcga taagctgata atcattttct gcacaatact ctagaagctt gacaaggagc
     2161 agattgcctg tagaaagtga agaataattt tcggtatcga aagcaggcat aaggtaatat
     2221 aggcgtggcg gacagctaag tccccaatgg acagctaggg gatttttatt cagcataagc
     2281 gcgcatatat gtgcggggat attatcattt tgcgaggctt ggatatagaa attgcgaaat
     2341 gcaggatcag caaagttgtc gtggactttt atctggcgaa agcgctcctg cttaaaagca
     2401 atcatggtat gcgtaaggcg ctctcgaagc gatttgtctc cagcgatgat aaaggagaga
     2461 gtaccagctt ctgatagacg gcgtttggct ctgcggaggt gcgcacgttc tttagtgttg
     2521 agacgcatta gtgggtcttg tgaaagctgc attgcgtgag cgctataatg gaggcgatgg
     2581 ccaaagggca atgcaagtag tgcttttgca aatataagag gtgtgccgtc agcccagaat
     2641 ttggggattc ggtcaagata tatggcacag cagtgttctt tcttcgcaat gtttttgaga
     2701 tgggcaaaga gctctaccat atcgaagtta gtctcattcg aaaagattgg tgcagcgtag
     2761 tcagatacgc catagccgag ccatattagc attgtgctgg ataaagcacc aaatcgcacc
     2821 tttttctttc caagagggaa gagagctgta ttctggcctt ttttgaaaac gataataaaa
     2881 ggggcaactc catgggcttt cccaatggta ttctgccaag tagaaagcca gggtcgagtt
     2941 tgaaaagcgt agatgcatgg actttgtaca aggctatccc agatagctgc aggtctatcg
     3001 atatcgtggt atatcgaaac ctgtagctgt tcattaatca tattccaagc ctcaatagaa
     3061 ctcctaaggt tagttcgccg agaagttttg tgttgcctgg aacaggagaa taaatctgta
     3121 ctttttggtc gacaagagtc tggcttgacg atacgccaat tgggtcgcgt agttcgagcg
     3181 catcaagggc aaggctgcca gtggcttttg caaagaactg ccaggggcta tgccctgtaa
     3241 ttgctcgagc tgaaatagta aatctttttt cgagcacagc gtcatctagc caggtgaaag
     3301 caacttgtgg atcgacccaa agatcgtgcc agggattggc ggggctattc gagccagaaa
     3361 gcctaaattc aaagaaggcc tcacattcga acttactaac agaaccgttc cagtcgaggc
     3421 gcatggcaag attgttctcg ccgtattggt agccgattag attcagttct ggagcaatct
     3481 ccttatagct tttaggatag cttggtctcc attcgtactc aaaacgagtt tctgggaaat
     3541 aggtgtaacc gtaggaactt tggatctgat tcgctgtagt cggagtgcgc atatctccag
     3601 gctcaaaggt gtattttgtt gcgccagcta gatggaggcc aaaagtcccc ttaggtgttt
     3661 ttattcttcc gcctagggcg agtgcaatct gccagggatt gttcgtccat ccaaaaagtc
     3721 ccaacatccc aaggtcgtcg acaaaagcct gcgcatagag gtcataagcc cctggctctt
     3781 tccaagtgac gaataatcct gcattgtagt tatcatcgta gttggcatac caaggccgac
     3841 ctcctgtgcc tctgacatat tgggtaaagt attgaggcat agggcttagg aaatattccc
     3901 aatcgaaagc tcggccggaa taaaccgaag agtcttcaaa gcctatcgta agccttccaa
     3961 aagaaaggcc aaagaccttg aagtttgccc ctcgatcggg aaagccggta taggagggat
     4021 catcgggcca agccggtgta tgcattgcag aatcgagagt aagcgagatg tatcgcgatt
     4081 cgtaaaagaa tcgatcatct ctatacacaa agtcgagcat cggcgctgca atgccattcg
     4141 cgttgataaa gagcgagtac ggcgaatcga ccacatcgaa atggcgaagt ctgccacctt
     4201 ttaagctaaa tgagtcacca aaaattcgga taccagcttc cttgatcatg aaattgaaat
     4261 tcccgagggc gcctgtggga ctatcggcga gccaagggcc atagacttgg tcgctggtga
     4321 gatcaatggc agcgataagc ccaatatgag gttgctcata ctgaagggat attgaggcat
     4381 ttggtgttag tcctcgtgct tcgatttctt cgcgataggt gcccgcgcgg taaggcaatt
     4441 ctgtagcgag agtgaagaga aggccttgct cggcaatctc gctgggctgc tggaatacgc
     4501 tttggctggg ctgctgtgct gctgcgcttt ggctggcctg ttgggcagac agcgtaaaaa
     4561 ttcccgagaa gataagaaat aaaagaatcg caattgctgg caaatatagt tttttcatct
     4621 atattcctac tatccattat taagtctata gcaattcaac tgacccgcta tgaagcttgt
     4681 ggctatttgg ctttgtttgt attcggtaat gaatacctgc tggtatcagt gaacagttcc
     4741 gaatgctatg gtacctgaga gcgcaatgtc aagctcgaag gccatcgagt tatcgcttcg
     4801 gacaaggctc ggtgcgatat cgaaagagaa caaacctgca gacagacgta aaggtagact
     4861 tattaaaaag cttttccaat gcggcgccgc aggcgttgtg ctgttgttat agacttgagt
     4921 cgtgataagg ttggcatcgg tattcatatc taaaaacaaa aggcgatagc caacaccgag
     4981 gctgagggga ttcttattat tattgcttcc aattgggata atgagctcac catggctctg
     5041 cgcccaagta acgccagggc catagggact tgtgagcggc aataggatcg atcctcctaa
     5101 gtagcgcgga aaccggtata taaagcgagc aaaataattg acatctccta tgctgggcca
     5161 ggcttggctc gcatcgtagt tgccccagag ccaatgagta gaaccagcct ccatatatat
     5221 tgcaaaaggt aaggctctta ctcgacctat ctggccgcta tagcgcacac ctgctatata
     5281 agcagagatg gtaggtgaac cagtatcgcc gatgccaact agattcaaat cgatatcgtc
     5341 taggcccagc tgaccagcga tagtaaggcc actaattggc tcccagctcg catcgaacac
     5401 catggagttg ttggtctgtg caaccgatgc ctggtggcgc gagccaatgg ggaaaaaatc
     5461 ggtgagcaca aagtggttgt tgagacggga catcattgca tggtctgtta cacctaaggt
     5521 tacccaaccg agatgacagg aaaatcggtt cattgcctcg acaattattg ttggcgtgcc
     5581 aggttgatcc tcaaagccat atccggcctc tggcgagata tcatttttgt cccatgcttg
     5641 cactgcctgg attgtggaca cgagccagtc gaaagcaaag caaccaagtt ttccttgagc
     5701 tcgtagcgca tcgtaatatg gtatccggcg cgatggaaga aagccgcctt cgagtatgcc
     5761 gttgtaatcg aggcggtcgc gtccaaagcc tatctcgaat attggagatt tccaatatag
     5821 gacggatcgt gcaactatgt tgccctctac atcaggtgtt gtgccattta gaggcagatt
     5881 atgaacaggg aaatagtctc ctgcccagct atgccgcaga tcgatttctg ccgcgacacc
     5941 aaggccctgg gcattgcata accatggatc gaagccctgt gcatcgaggg cgctcccagc
     6001 ttcaatgctc agaactgcaa agggcaattg gccaaaataa ttccgttgga tatcgatgcc
     6061 attggaatat gctactgatg ggtactgact cacaggaggt tcgatgcgga cattatcgct
     6121 tcttaggctg tattcgatat ttatactaag cacgatgcgc cattggagcg atgtgggcct
     6181 agattgagtt tgcgcaaaaa ggaaatttct cccaaagagc atccttgatc ccgaaaacaa
     6241 aaatatagag aaaataaaat atagaaagaa tagattggaa tttgccgcgc gcattttgca
     6301 tcgatgtttc atggtttcat tctcctttaa tcgcgtttag ctagattgcc cttttgatcg
     6361 tgcttatccg atcatttttc atcaaagccg catggcgttg aggctcgctc ttctgcgcct
     6421 gcaatgcgcg aggtcggaaa gcccacttat ttttgcaaga aagagcgaaa atggaagatc
     6481 gggctcaagt actgtgcgat ggattgctcg cgcatcttca cctactctga atagcgagga
     6541 ttgggtcgta aaaatccatg aaaaacctgc atcgcagagt agctgtaagg cgtctgcacg
     6601 gtaggtatcg gattctcccc ctacccacgc aaatgcattg acagggtagc caaggtgctt
     6661 ttcgagctct tttttagctt ctacaatctc gtcaaagagt atagcggtat cgagagtcgg
     6721 acgcatgcga acatggtgag cggtatggca accaattaca tgaccgcgct tcgcaaggtc
     6781 gaggatctca ttccagctca tcgcaacgtg gctcgaaacg aatgaatgct gagccttccc
     6841 tagatgagct gtttttttat gcagggaaat ttcatgtgat gcacaaaagc cttcttgaaa
     6901 ttttggtgcc aagccaggaa gtgtacttgt aacaaaaaac catccacgca taccacgggc
     6961 ttcgagaaga ggcgcagcaa tttcgtagtg ttccataagg ccatcgtcga agctaataat
     7021 aaggcctggt ttgcattgca tgctggcttg cttgttcgat attttcgctt ttccagcaga
     7081 atggccatac tttctgcgca ttccagatac ctcgcaagag ccttgatcta agaaccgcgc
     7141 aaagccttct gcatcgagtg ggatatagtg ctttgcaaag taatcgagct ggcgcgaaaa
     7201 gctggactct gaagatttag gtacagcatg atatgcgacc gccctcagag tactgccaat
     7261 catggcattt tccattacaa tggagcctcc aattatagat ggagccctat ccccagccat
     7321 atttcggtat cgccccaatc tagagtttta gtttccaggt agtgttctat attgtagccc
     7381 aaagagagcc gataggtgaa cgatcgcgca tgttcaggaa atatcaccac atcgagtcct
     7441 cctcccatca gaaggttgtc tatagaccat agagggttgg caggattcgt tcgcacatat
     7501 cctaaatcga agaaaggaat aatgaagatc tccgcatcca ttgcttccca ttggaagaac
     7561 ttgccctgag caaaatttac cggtatctgc aaattgacta ttgcagcgag atcgccatat
     7621 attggccctc gaattccacg aaggtaggtg ctcgcatcca catcatcgaa gtagcttagg
     7681 tcgttatata agctccaccc cgcatcccag cgccctatgg cccgaaaatc aattcccaga
     7741 aggttgaacc aagagtaaaa ggctgaaagc tgagaggaaa gaacaaggtc tagaggtcgg
     7801 gtagaatgtt tcgatccaat atggaacata tacgattcag ataaagaagc actgctgcct
     7861 tttctgaagt tgcccttcca tttgatgcta ttgtatccca ctgaaccaga tgcacttagt
     7921 cctgcatcgt ataccatgcc tttggccaga tcataggata aagaaagacc tgcggaaggt
     7981 gtaaatgcga gcatagatgc cgaagctact gagaaaaggg gaatacgaag agagagcgtt
     8041 gtttctgcaa tagctagata tgaccaagaa ccggcatcat tttcttgttt atatga
//
LOCUS       DAOSOB010000070         8011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_8987_length_8011_cov_79.085721, whole genome shotgun sequence.
ACCESSION   DAOSOB010000070 DAOSOB010000000
VERSION     DAOSOB010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8987_length_8011_cov_79.085721"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PLS88_07345"
     CDS             complement(<1..684)
                     /locus_tag="PLS88_07345"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOU07196.1"
                     /translation="MTQELWLFILMVGAFAAGCFAFKWPVSVAMLVASALGAIVGGVQ
                     NPVRHLVEGTFGYLDTILTIATAMMFMTGFRDSGALHGLTAAVIKKFNKWPALLMILL
                     TFVIMVPGMITGSSTASVLTAGAVVAPMFIAMGMSTVEAGALISIAAILGMIAPPVNI
                     PVMIIGGGIDMPYVGFEIPLILLTFPLAIVFALWFGWRNVRKVDRSNVLVTLDFSELN
                     KYGGLRLFSP"
     gene            complement(695..>1138)
                     /locus_tag="PLS88_07350"
     CDS             complement(695..>1138)
                     /locus_tag="PLS88_07350"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOU07197.1"
                     /translation="QSADVEMVKTLMTRAKLNFTMDPLVKAQALASTNAKTLVIVIGG
                     SSKGLGAAGISADAELERTKALLAEAKKRGMKIIGLHVGGEARRGELSDRFINEAVPS
                     CDYFIVVEEGNKDGLFTKLCGNKIPLDIVQKISQIGEPLAAAFLK"
     assembly_gap    1139..1238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1307..2992)
                     /gene="ggt"
                     /locus_tag="PLS88_07355"
     CDS             complement(1307..2992)
                     /gene="ggt"
                     /locus_tag="PLS88_07355"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOU07198.1"
                     /translation="MKKAIFAFMLLVAAVTALSAQLPVNLYGRAAVGENGVVAAAKPE
                     ASQVGVDILKKGGNAVDAAVATGFALGVLEANANGIGGGGFMIIKMVDMDEPVVIDFR
                     EKAPGKATPTMYLGPDGKVIPNSTIEGGLSVGVPGEVKGLLYALENYGSGKLSRADII
                     QPAIQWALQGIPVTVNLESIIKDNYGKLVKYENGAQIYLKDGLPYEVGDVIYNPDLAK
                     TLAKIVKEGEDAIYKGPIAQAMVDEVQKRGGILTLEDLANYEIKVRKAVEGDYRGYKV
                     YSVPPASSGGTHLIEILNILENFDVVKLGFQTPQASHLWSEILKLTFADRAKYMADTD
                     FVQVPLKGLTDKEYAKQQAARINMNASMSSPQPGDPFKYESGSTTHFSVMDKEGNMVA
                     VTKTINYFFGSGVVIPGWGFIMNDEMDDFVPTPGSVNSVEPGKRPLSSMSPTLVLDPE
                     GRSFMAIGSPGATRIFPTVAQVISNVIDFGFPIQEAILAPRLFQAASGNLNLEGRYPV
                     SALDGIKKLGHDVTIRADWDSYFGGVHAVLYDYDKGLLYGGADPRRDGQAAAF"
     gene            complement(3133..3777)
                     /locus_tag="PLS88_07360"
     CDS             complement(3133..3777)
                     /locus_tag="PLS88_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07199.1"
                     /translation="MDRERISKPVILLFILTLVLSIATVVTVQVFSRYKPDIRSKFNL
                     YLEKLEPQSMLVVATTQERYEASKEFTAKLLAIFNIRAKIRLSAVADVTYIIPASDPS
                     AWSIDWNSKARKIVLSSPAPDCLLPAVHTETIEIITENQNILTNTIFKLKEEAAHMQA
                     ELSNDFLVRARATLTEPAVRATIEDGLKGFAIAFCESAHLGKPTSIEIHLGESH"
     gene            complement(3799..5094)
                     /locus_tag="PLS88_07365"
     CDS             complement(3799..5094)
                     /locus_tag="PLS88_07365"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HOU07200.1"
                     /translation="MCDTLILHDNRGKPFLFGKNSDRHPEEPQALVYIPPHEPKNVQV
                     REGIEYSDKGYAFLLSKPSWINGGEMGLNSKGLAIGNEAVFSRFKPDKNGVLGMDILR
                     AALSACATSEEALDFISSFIERFPQGGNGAYKGKLFYNNSFLIADPEKAYILETAGAR
                     WAWRKAMIADAISNVYCIEDDYERLDAVTGKEISAGDDRVARPNETDPSQKEQRASWK
                     KYVEDKKYLLFTQGEQRRSTSLSSLMRIAAGSDKTEQGQVLSSSAIEPFFAILRSHEG
                     APKPGAFLNRMKNVCIHPGIFPKSATTASMAVEYCSGGAIIWYTDSSYPCISLYKPAL
                     LKDGRFYALWKSLLEENKAEKSYTYWGKRKDWASQPHKLALSSQQSFVQSRDAGQKSI
                     VAIAHQALSSMIKEKTTPERMLSVYASEVAAIVGEWEEH"
     gene            5230..6123
                     /locus_tag="PLS88_07370"
     CDS             5230..6123
                     /locus_tag="PLS88_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU07201.1"
                     /translation="MILRIVVFVFALINISAFVTVNNLLYAQTDYVLREPSPPQNPAS
                     VGKFQFSSPGLLSQSQEILPGIPFPKGMMLPAVTTFFELRDNVYKSATPESIEAQAVS
                     LLKMLSIQPISGSNQALLEARISYLVGRSWNDHKDKKKAITWFERAVEAAQKMIGLEG
                     ETPTALVTLAEPLGELSILKDLGFLIKNGPKVGQYASKALESDPKNIKALLLKASALA
                     YPPPIWGGDYKKALEIYASVLSIAQNGLPRDVLFDLRVGIATAYSNLKLSENAAWWFD
                     AALELYPENPYAKSELEKLPL"
     gene            6120..7259
                     /locus_tag="PLS88_07375"
     CDS             6120..7259
                     /locus_tag="PLS88_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU07202.1"
                     /translation="MINALLTAYGLIIFAACVFFFFITVSNILWLMHTLTIERKKTGP
                     SVAVLIPARNEALRIRPCIDSLLCQNYSSYQIYVIDDNSSDETWNILQSYMIRFPSKF
                     KAFKAEPLPEGWYGKPHALQKLAAHVKEDYLLCTDADTIHKPDSISKAIAAADKYKAD
                     LITGYPHHSMPTFAEAAVVPSMYILTMLGMPLYLIPATKSPLFSHAIGQFMLFKRSFF
                     EKIGGYEKVKHEATEDVRLSRIIKEHGGKIAFIDLKDIVECRMYTTYKEAIQGIAKNA
                     YDYLGKNLFILFSGTVAVPLVFYIPLIVLFFDIPWLGPAIPYLKASAIITFYSWVLET
                     IDRRLPWYIPFIYPLIFVNTMSALWRGFRLVNKEGGIEWKGRKVK"
     gene            7256..>8011
                     /locus_tag="PLS88_07380"
     CDS             7256..>8011
                     /locus_tag="PLS88_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU07203.1"
                     /translation="MRYRKGAPLVSDTPLSRLIGDILVAFIGLIGWPLCRLLYNIKIE
                     RIGWEKRRRLPSRAILVSNHCMPLDPVFHGLSILPRRTFFTLLEETCEAPVIGSLVRL
                     LGGIPLPRNKKRLGDIERAVEHALSTRGLVHFYPEGECFLGNQNIFPFKAGAVFFAIK
                     FGVPVIPIVTILKRANGQKSKRIQVNVHMLEPIMPKTFLSKPISSIARDPPQENHREM
                     LSCAIECTREIHALMQAEIERSSGDKSLYKGPMP"
BASE COUNT         2067 a         1906 c         1768 g         2170 t
ORIGIN      
        1 gggagaaaat agcctgaggc cgccatattt attcagctcg ctgaaatcga gggttacaag
       61 cacatttgaa cgatcgacct tgcgcacatt tctccagcca aaccaaagcg caaacactat
      121 ggcgagcggg aatgtcagga gaataagggg tatttcgaag cccacataag gcatgtcaat
      181 acctccgccg atgatcatga ccggaatgtt cactggcggg gctatcatcc ccaaaatcgc
      241 cgcaatcgaa atgagcgctc ccgcttctac cgtagacatg cccattgcaa tgaacatcgg
      301 agcaaccacg gcaccggcag tcagcaccga cgcagtagaa ctacctgtga tcatccctgg
      361 caccataatc acaaaggtca ggagaatcat aagaagtgca ggccatttat tgaacttctt
      421 tatcactgcc gccgtcaaac catgcaaggc tcccgaatcg cgaaagccag tcatgaacat
      481 catggccgtc gcaatcgtga ggattgtatc gagatagcca aatgttccct ctaccagatg
      541 tcgaactgga ttctgtacgc cgccaacaat ggcacccagt gccgaagcaa caagcattgc
      601 aaccgatact ggccatttga aagcaaaaca tccagcggca aatgcaccaa ccatcagaat
      661 gaaaagccat aattcctgtg tcacatagac ctcgctattt tagaaaagcc gcagccagag
      721 gctcgccaat ctgagagatt ttttgcacta tatcgagagg aatcttattt ccgcacagct
      781 tggtaaaaag gccatcctta ttgccttcct caacgactat gaaatagtcg caggaaggga
      841 cagcctcatt aataaagcga tctgaaagct cgccacgccg tgcttcgcca ccaacatgca
      901 gaccaattat tttcatgcca cgttttttag cctcggcgag cagagctttt gttcgttcga
      961 gttctgcatc tgcggagata cctgctgcac ccagcccctt ggatgagcct ccaatcacta
     1021 tcactagcgt cttggcattc gtgcttgcaa gtgcctgagc tttgactaat ggatccatgg
     1081 taaaattcaa ctttgctctt gtcatgagcg tttttaccat ttcgacatcg gcgctctgnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct atctcagcgg ccggctccat
     1261 tgtctaagac attctaatgc gatgcagatt tactgcattg atttgctcag aaggccgcag
     1321 cttgtccatc gcgacgagga tctgcaccgc catagagcaa tcccttgtca taatcataca
     1381 aaaccgcatg cactccgcca aagtaggaat cccagtcggc gcgaatagtc acatcatgcc
     1441 ccagcttttt gattccatcg agcgcgctga ccggataacg cccttcgaga ttcaagtttc
     1501 cagaagctgc ttgaaagagc cttggcgcaa gaattgcttc ctgtatcggg aatccgaaat
     1561 cgataacatt ggatatgacc tgagcaacgg tggggaatat tctcgttgcg cctggagatc
     1621 caattgccat aaaggagcgg ccttcaggat cgagaacgag agtggggctc atgctggaaa
     1681 gaggacgctt gccaggttcg acagaattga cagaaccagg agtcggcaca aagtcatcca
     1741 tctcgtcgtt cattatgaat ccccagcctg ggataacgac gcctgagccg aagaagtagt
     1801 ttatcgtctt ggtaacagct accatattcc cttctttatc cattacggag aagtgagttg
     1861 tagagcccga ttcgtatttg aatggatcgc ctggctgcgg agagctcatc gatgcattca
     1921 tattgatcct tgctgcttgc tgcttggcat attccttatc ggtaagtccc tttaacggaa
     1981 cctggacaaa atcggtatca gccatgtact tcgctcggtc tgcaaaagtg agctttaata
     2041 tctctgacca gagatgagat gcctgcggag tctggaagcc gagctttaca acatcgaagt
     2101 tctctaagat attgagaatc tcgatgaggt gagtgccgcc agaagatgct ggaggcactg
     2161 agtagacctt atatcctctg taatcgccct ccactgcctt gcgaactttg atctcgtagt
     2221 tggcaagatc ttcgagagtg aggatgcctc cgcgtttctg gacctcatcg accattgctt
     2281 gagcgattgg gcctttgtaa atcgcatcct cgccttcctt cacgatcttt gccaatgtct
     2341 ttgctaggtc gggattgtat atgacatctc ccacctcata aggaaggcca tcttttagat
     2401 agatctgggc tccattctca tatttgacga gcttgccata gttgtctttt atgatggatt
     2461 ctagattgac cgtgactggt atgccttgca atgcccactg aatggctggc tgtatgatat
     2521 cggctcgaga caatttgcct gagccatagt tttcgagtgc atacaatagg ccctttacct
     2581 cacccggtac tccgacggaa agcccgcctt ctatggtcga attgggaata accttgccat
     2641 ctgggcctaa atacatggtc ggcgtggcct tgccgggagc cttctcgcga aaatcgatca
     2701 caaccggttc atccatgtct accattttga tgatcataaa accgccaccg ccgatgccat
     2761 tggcgtttgc ttcgagcacg ccgagcgcaa agccagtcgc aaccgcggca tcgactgcat
     2821 taccaccctt ctttagaata tcgactccaa cctgggatgc ttcaggctta gctgcggcaa
     2881 ccactccatt ttctcctact gctgcacggc cgtatagatt gactggtagt tgggcagaaa
     2941 gcgcagtaac cgctgctacc aacaacataa acgcaaaaat agcttttttc ataagctcct
     3001 ccttcaattc ttaccctgta atatttaatc aatttaagat tcttatagtt tatggcaggt
     3061 gtcaaccctt tgtcgggcaa ttgcgctcaa tattcgcaaa tcatccgagc tcgattcaat
     3121 ccaggctgtt ttttaatgcg attcacccag atgaatttcg atcgatgttg gttttccaag
     3181 gtgggctgat tcgcagaatg caatagcaaa gccctttaag ccatcttcga ttgttgcgcg
     3241 aaccgctggc tctgtaagcg ttgctcttgc tcgaacgagg aagtcattcg aaagctcggc
     3301 ctgcatgtga gcagcttctt ccttgagttt gaatattgta tttgtaagga tattttgatt
     3361 ctctgtaatg atttcgattg tctcggtgtg tacggctggc agaagacaat ctggcgcagg
     3421 gctcgagagg acaatttttc gggctttaga attccaatcg atagaccagg cagaaggatc
     3481 tgatgcaggg attatataag ttacatcagc tacagcacta agacggattt tcgctctgat
     3541 attaaagata gcgagcagtt ttgctgtaaa ttccttagaa gcctcatagc gctcctgggt
     3601 tgttgctacg actagcattg actgaggttc caatttctct agatatagat tgaatttaga
     3661 tcgtatatcc ggcttgtagc gagaaaatac ctgtacggtc acgactgtcg ctattgatag
     3721 aacgagagtc aagataaaaa gaagaataac aggtttagag atgcgttctc tatccatctc
     3781 taatttctcc agtgttcttc agtgttcttc ccactcaccg actattgccg caacctcgct
     3841 agcatataca gaaagcatac gctccggggt agttttttcc tttatcatcg aagaaagagc
     3901 ttgatgagcg attgcgacaa tcgatttttg ccctgcatct ctcgactgca caaaggattg
     3961 ctggcttgat agggcgagct tgtgtggctg cgaagcccaa tcttttcgct ttccccagta
     4021 ggtatagctt ttttcagctt tgttttcttc caagagagat ttccagagag catagaaccg
     4081 accatctttc aatagagctg gcttatagag agaaatgcag ggataggaac tatctgtgta
     4141 ccatataatt gcgccgccgc tacaatattc tactgccatc gaggctgtag tagcgctctt
     4201 tgggaaaatg ccaggatgga tgcagacatt tttcattctg tttagaaatg cacctggttt
     4261 aggagctcct tcgtgcgagc gcaaaattgc aaagaaaggc tcgattgcgc tcgaagaaag
     4321 tacctggcct tgctcagtct tatccgatcc cgcagctatg cgcataaggc tagaaagtga
     4381 agtcgatctt cgctgctctc cttgagtaaa gagaagatat tttttatctt ccacatattt
     4441 tttccatgaa gcgcgctgtt ctttttggct cggatccgtt tcgttaggac gagctacccg
     4501 atcgtctcca gctgaaattt ccttgccagt aacagcatcg agacgctcat agtcgtcctc
     4561 tatgcagtag acattcgaga ttgcatcggc gatcatcgct ttccgccagg cccagcgcgc
     4621 tccagcagtc tcgaggatat aggctttctc agggtctgct ataagaaaac tattattata
     4681 gaaaagtttg cccttatatg ctccattgcc tccttgtgga aatctttcta taaaagagct
     4741 tataaaatcg agggcctctt ccgaagtagc acaggccgat aacgcggccc ttaagatgtc
     4801 cattccaagt acgccatttt tatctggctt gaagcgcgaa aaaactgcct cgttgccaat
     4861 ggcaagaccc ttgctgttga gacccatttc tcccccatta atccaagaag gcttggagag
     4921 aaggaaggca tagcctttat cagaatactc aattccttcc cttacctgaa catttttggg
     4981 ttcatgagga ggtatataga caagggcttg aggttcttct gggtgccgat ccgaattttt
     5041 gccaaataaa aatggtttac cccggttatc atgcagaatt agagtatcac acataggctt
     5101 gcatacctcc tcagggatta gatcccggcg caagtcgctg aattatccta tatcaacttt
     5161 taatattccc gcaagcgcaa aagtctgcta tcctggttta taatggattc aaataagaca
     5221 catttagatg tgatattgcg aatcgtggtg tttgtttttg ctctaataaa tatttcggca
     5281 tttgtaaccg ttaataatct gctctatgct cagaccgatt atgtccttag ggagcctagt
     5341 ccaccgcaaa atccagcttc agtcggcaag ttccagttca gcagcccagg gcttttatct
     5401 caaagccaag agattctgcc tggaatccct tttccaaagg gtatgatgct acctgcagtt
     5461 actacctttt ttgagctccg cgacaatgta tataaaagcg ccacccccga atcaatagaa
     5521 gcacaagcgg tttcactgct caagatgcta agcattcagc ctatatccgg ttccaatcag
     5581 gcattgctag aggcgcgtat ttcatatttg gtcggtcgat cctggaatga tcataaagat
     5641 aaaaagaaag caattacttg gttcgagcgc gctgtagagg cggctcaaaa aatgatcggc
     5701 ttggaagggg aaacgcctac agccctcgtg acgcttgctg agccattagg agagcttagc
     5761 atcctaaaag atcttggttt cctaatcaaa aatggtccca aggtaggcca atatgccagc
     5821 aaagcgctcg aatcagatcc gaaaaatatc aaagccttgc tgctcaaagc cagtgccctg
     5881 gcttatcctc cacctatttg gggcggcgat tataaaaaag cattggaaat atatgctagt
     5941 gtcttatcta tagcccagaa tggcttgccc agagatgttc tattcgatct acgcgtcgga
     6001 atcgctaccg cctatagcaa tctcaagctt tccgaaaatg cagcatggtg gttcgatgcc
     6061 gcgcttgaac tttatcccga gaatccttat gcaaaaagcg aattggagaa actgccatta
     6121 tgatcaacgc tttgctcact gcttatgggt taataatctt tgctgcatgt gtgtttttct
     6181 ttttcatcac ggtttctaac attttatggc ttatgcacac actgactata gagcgaaaaa
     6241 agactggacc atctgtcgct gtacttatcc cggcgcgcaa tgaagctttg cgcattcgtc
     6301 cttgcatcga ttctctgcta tgtcagaatt atagttctta ccagatctat gttatcgatg
     6361 acaactccag cgacgaaacc tggaatatcc tgcaaagcta catgataaga tttccatcca
     6421 aattcaaggc cttcaaggcc gaacccctgc cagaaggctg gtatggcaag ccacatgcgc
     6481 tacagaagtt agctgctcat gtcaaagaag actaccttct atgcacagat gcggacacga
     6541 tccataagcc agattccata agcaaagcaa ttgcagccgc agataaatac aaagccgatc
     6601 tgatcactgg ttatccgcac cactcgatgc cgacattcgc agaggctgct gtcgttcctt
     6661 cgatgtatat tcttactatg cttggcatgc cactctatct gattccagct acaaaatctc
     6721 cactcttcag ccatgctatc gggcagttca tgcttttcaa gaggtctttt tttgaaaaga
     6781 ttggcggcta tgaaaaagta aaacacgaag cgacagaaga tgtgagatta tcgcggatca
     6841 taaaagaaca tggcgggaaa atagccttta tcgatcttaa agatatcgtg gaatgccgaa
     6901 tgtatacgac atataaagaa gctattcagg gcattgctaa aaatgcctat gattatcttg
     6961 gtaagaatct ctttattctc ttttctggaa ccgtggctgt gccgcttgtc ttttatattc
     7021 cattgattgt gctttttttc gatattccat ggcttggacc ggctattcca tatcttaaag
     7081 cttcggcgat tattacattt tactcctggg ttcttgaaac aattgatcgc cggctaccat
     7141 ggtatatacc atttatctat ccgctaatct ttgtgaatac aatgtcagcg ctttggcggg
     7201 gatttcgcct tgtcaacaag gaaggcggaa tcgaatggaa gggcagaaaa gttaaatgag
     7261 ataccgaaaa ggtgcccctc ttgtctcgga cacacctcta tcgcgtctta ttggcgacat
     7321 ccttgtggca tttattggtt tgataggatg gcctctctgt cgtcttcttt acaatatcaa
     7381 gatcgaaagg atcggctggg agaaaaggcg tagattgccg tctcgagcta ttttggtaag
     7441 caatcactgc atgcctctcg atccggtctt tcatggtctt tccattttgc ctcgtcgtac
     7501 tttttttact cttctcgaag aaacctgcga ggcgccggtt attggctcat tggtgaggct
     7561 gcttggcggg attccattac ctagaaacaa aaaaaggctt ggagatatcg agagggctgt
     7621 cgagcacgcg cttagtactc gaggcctagt ccatttctat ccagaaggcg aatgtttcct
     7681 agggaatcag aatatattcc cctttaaagc gggtgccgtt ttttttgcga taaagttcgg
     7741 agtaccggtg atacctattg ttacaattct aaagcgagct aatggccaaa aatcgaagcg
     7801 tatccaggtc aatgtgcata tgcttgagcc aatcatgcca aagacttttc tgtctaagcc
     7861 tatatcgagc atagctagag atccacccca agaaaatcat cgcgagatgc tctcttgtgc
     7921 aatcgagtgc actagagaga ttcatgctct aatgcaagcg gaaatagagc gctcaagcgg
     7981 cgataaatca ctatacaaag ggccaatgcc g
//
LOCUS       DAOSOB010000071         7992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_9019_length_7992_cov_92.485322, whole genome shotgun sequence.
ACCESSION   DAOSOB010000071 DAOSOB010000000
VERSION     DAOSOB010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9019_length_7992_cov_92.485322"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1283
                     /gene="metK"
                     /locus_tag="PLS88_07385"
     CDS             93..1283
                     /gene="metK"
                     /locus_tag="PLS88_07385"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOU07204.1"
                     /translation="MTRALLRPFTSESVSEGHPDKICDQISDAVLDACIAKDKHSHVA
                     CECFATTALVIVGGEITTRTYVDIQELARSVVRDIGYDNPEYGLDWASMAVLNSIHNQ
                     SPDIAQGVDGHGLKEYEGKQGAGDQGMMFGFACNETSELMPMPIMLAHKILQKAAAVR
                     KAKEIAWMRPDGKSQVTVEYDGYTPVRISAVVLSQQHDEKNGGGHYLSYEEIRETLIE
                     RIIKPVLEPTGLLDAGTRYFINPTGRFVIGGPLGDTGLTGRKIIADTYGGMGRHGGGS
                     FSGKDPTKVDRSAAYMVRYIAKNVVAAGLADRCEIEVAYAIGVPEPISVFVETFGTAK
                     VDEEAIERAILQVFDLTPKGIIETLELKNPIYRATSVYGHFGRDSFSWEKTDRVEDLL
                     KAIR"
     gene            complement(1367..>2166)
                     /locus_tag="PLS88_07390"
     CDS             complement(1367..>2166)
                     /locus_tag="PLS88_07390"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOU07205.1"
                     /translation="NPYSTVELFDDIEFSEPDPARLAQSIGDRLIQVGDRALAKPMPK
                     LSGIPLILRGKEAEEIFSWFFENSRTEAIYSKASPFRTGVNVQGGNENIEAEHKVYGA
                     IIEPLDLWAEPVISGLPASAAFDSDGFPLERTLVIENGILKTLIGSVRHADWLGVPRK
                     GAFSLFSVPGGSMSLEEMHAIPYIEPVMFSDFRLDSMTGDFGAEVRLAYYFDGKNVTP
                     VTGGSISGSMSTMRTTMRRSKETATMSRSVCPIAVMLQGAIIAGARY"
     assembly_gap    2167..2266
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2267..3008)
                     /locus_tag="PLS88_07395"
     CDS             complement(<2267..3008)
                     /locus_tag="PLS88_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07206.1"
                     /translation="MIDTIKKAIAIINESSSKNLHASARIGAAYAAAPSPVDSESPVA
                     QAIADWRLVERNIQRQERYYIVDRAEQARSIDEVVYSLTVYVDSIDDVSHPSACGNEN
                     TPDEASANSAPSSEKVDGSEAPRRFRGEATITIFPSFSLEECVVKIRQAVFAASKSHN
                     PWFSLPAPSPLSVQVPISGFEALSFEDRVKSIRSALYSPFEHMNQPKAVPIVDSLAMP
                     KNVNPRINSLELFISKEQRRLLNSQGIDY"
     gene            complement(3008..3961)
                     /locus_tag="PLS88_07400"
     CDS             complement(3008..3961)
                     /locus_tag="PLS88_07400"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOU07207.1"
                     /translation="MAQSRYECVDVSRLFVSPKRSLMQSFLWSQAYLFGLARSGEISK
                     MSYRSASGRKGLEILANLESSVPELSKELADLLDAVPMEPGEYEVILDPDMAGTLAHE
                     AFGHGVETDMFFKKRARAVDYLDKTVGSELVTMYDGAAGVDQTGSYLFDDEGVFSSKT
                     CVIDKGILKGGISDTLSALALNLPLTGNGRREAYSHKAYARMTNTYFAPGISTLEDMI
                     ASVKHGWKLSQLDAGMEDPRNWGIQLICMVGREIKDGKFTGRVASPIVCSGYVPDVLS
                     SVSMVSNDFELGGSGACGKGHKEYAKVSSGGPYVKTRMRLG"
     gene            complement(4010..4513)
                     /locus_tag="PLS88_07405"
     CDS             complement(4010..4513)
                     /locus_tag="PLS88_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07208.1"
                     /translation="MQKRSHSPFLLAAKPLLDQIINLLGPHFDYISVLATDDRGLSYV
                     ALPGETRTYEPMWVQRGFVFRAQRDGKVVEYACSTLPFDVSGISDLAYEAIKSLSLSG
                     ADSEATSKASSIAASAASIIQTRLEVLLTDPTLIEFPKIPDIPATEEYCGEIQEDPFV
                     TDPESIL"
     gene            complement(4548..5114)
                     /locus_tag="PLS88_07410"
     CDS             complement(4548..5114)
                     /locus_tag="PLS88_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU07209.1"
                     /translation="MLVPESFELTNIWLRRPRLSDAPAIFEYGSDPEVARFADWPRLT
                     SLEPLLETLRRREILWNEGLEYYWIITLKDSDKAIGAISCRIAENAAEIGYLLNRKYW
                     GRGYATQAARAIVDWALSQAEVSLVWATCDVENVASIRVLERIGMHREKILPNYRIRP
                     QISGQARDAYLYVRISKTGTRKQKQHNI"
     gene            complement(5124..5648)
                     /locus_tag="PLS88_07415"
     CDS             complement(5124..5648)
                     /locus_tag="PLS88_07415"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOU07210.1"
                     /translation="MITIRDIVQYYAQYNLNANTRMIAIISDMIQDPINSPLKGYFLK
                     ALGTLLEHIYLTDLSWLKAFMELGNYGKGIEEDLGFALEPDKSLFQNFEDFTLKRQKL
                     DMLIVEFTSKVEEEFFSKKLTIKNSEGEEAECEIWKAIIQFFNRQTHYRGQISNILDQ
                     LHIPNDFSNMILIS"
     gene            5867..7282
                     /locus_tag="PLS88_07420"
     CDS             5867..7282
                     /locus_tag="PLS88_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU07211.1"
                     /translation="MASLGNKARDISFFLEAKKPAEVRKAIFKLALPVMLEFFLMMLV
                     QMIDMIQVGSLGPWAIAATGLAAQPTMLIFSVFSALVVGTTALVARASGAGDREYACR
                     VTRQSFVIVLLLGVVVTAAGIPASRTIIRLMGANVDTIKPATIYMQIVIAGTVFTVGN
                     MVLAASLRGVGDVITPMISNIVANIVNIFLNWVLINGKLGFPRLEVAGAAIATSFSRL
                     IAFCITMHAVYARDKYISISIHDSYRFESNIMRDVLNIGTPAAIEQLIMRTGALLFAR
                     LIAGFGTIMFAAHQIAINIDSLGLVPAMAFQMAATTLVGQGIGAKKPELSEEATRQSM
                     FISIIIMSLVGVVYFFLGKYIATIFTRDAQVIALGENALKILAFSQPFAAVYFVVAGA
                     LRGAGDTKYAMYASAIGVWAIRLVMGYLLADLLDMALVGAWIAVTLDMAVRMVLVLKR
                     FSSGVFKKYSKEYSEDSEVMI"
     gene            7393..7698
                     /locus_tag="PLS88_07425"
     CDS             7393..7698
                     /locus_tag="PLS88_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOU07212.1"
                     /translation="MPEFSTAFSVQKSDRRLTHQELVRAIRFMIAAEYEAIQLYMQLA
                     ESIDNDLAIEVLEDIANEERVHAGEFLRLLQEIAPDESKFYKEGAEEVEEEIKKAQK"
     gene            complement(7810..>7992)
                     /gene="glnQ"
                     /locus_tag="PLS88_07430"
     CDS             complement(7810..>7992)
                     /gene="glnQ"
                     /locus_tag="PLS88_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695655.1"
                     /note="similar to ATP-binding component of ABC
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HOU07213.1"
                     /translation="DLAHEGMTMLVVSHEMGFARAAANRIIFMDAGQIVEEAPPDTFF
                     TSPREERTRRFLEHIL"
BASE COUNT         2094 a         1713 c         1963 g         2122 t
ORIGIN      
        1 aagccaggtc ggcagaagtt ttccaagcgt cctggtcccg ccgatttatc cgaattgcgg
       61 gcgggtaaaa atatctgcta aagagggcta aaatgactcg agctcttctt cgtcctttta
      121 cttccgaatc tgtgtccgaa ggacacccgg acaaaatctg cgatcagatt tcagatgccg
      181 ttctcgatgc ttgtatcgca aaagataagc acagccatgt ggcttgcgag tgctttgcta
      241 cgaccgcgct tgtgattgtc ggaggggaaa ttacaacgcg aacctatgtc gatatccagg
      301 aacttgcacg ctcggtggtg cgcgatatcg gttacgacaa cccagaatat gggctcgatt
      361 gggcatcgat ggcggtactc aactctatcc ataatcagtc tcccgatatc gcccaaggag
      421 tggatgggca tggtctcaaa gaatatgaag gcaagcaagg agcgggcgat caaggcatga
      481 tgttcggttt tgcctgcaac gagacttccg agctaatgcc aatgcctata atgcttgccc
      541 ataagatact tcagaaagca gcagcggtgc gcaaggcaaa ggagatagca tggatgaggc
      601 cggatggcaa aagccaagtc acggttgaat acgatggata tacgccagtg cgaatctcgg
      661 cggttgtgct ttcgcagcaa cacgatgaga aaaacggcgg cgggcattat ctaagctatg
      721 aagagataag agaaacgctc atcgaaagaa ttatcaaacc cgttcttgaa cccacagggc
      781 ttctcgatgc gggcacgaga tactttatca atcctactgg gcgatttgta attggcggcc
      841 ccttgggcga tactggactt acgggacgaa agataattgc cgatacctat ggcggcatgg
      901 gacgccatgg cggcggatca ttctctggca aggatcccac taaggtagac cgatccgcgg
      961 catatatggt gcggtatatt gcgaagaatg tggtagctgc agggctggcg gaccgctgcg
     1021 aaatagaagt ggcctatgct atcggagttc cagagcctat ttccgtattt gtcgagacat
     1081 ttggtaccgc caaggtcgat gaagaggcga tagagcgagc tattctacag gtattcgatt
     1141 tgacaccaaa aggcataatc gagacactgg aactaaagaa tcccatttat cgagcaacat
     1201 cggtctatgg gcactttggg cgcgatagct ttagttggga aaagaccgac agggtggaag
     1261 acttgctcaa ggccatccgc tgagaaacag tcgaactatc ggataagcca caaagcgacg
     1321 gcttccgacc gataactttt gatcgacagc ttctaatcga caacttctaa tatcgggctc
     1381 ctgcaataat ggcgccctgg agcataacag caatagggca cactgagcga ctcatcgttg
     1441 cggtttcttt gcttcggcgc attgtggttc gcattgtgct catcgaaccg gatatcgagc
     1501 ctcccgtaac tggagttaca ttcttcccgt cgaaataata cgccaagcgc acctctgcgc
     1561 caaagtcgcc ggtcatcgaa tccagccgga agtcgctgaa cataacgggc tctatgtatg
     1621 ggatagcatg catttcttca aggctcatag aaccgccggg cacagagaaa agcgaaaagg
     1681 ctcccttgcg tggaactcca agccagtctg catgccgaac agatcctata agggttttta
     1741 agataccatt ttctatgaca agcgtacgct ctagaggaaa tccatcgcta tcgaaggctg
     1801 ccgaggctgg gagtcccgat atgacaggtt cggcccatag atcgagcggc tcgattatag
     1861 cgccatagac cttgtgttca gcttcaatgt tttcgttgcc gccctgcaca ttgacgccag
     1921 ttctaaatgg cgaagctttg gaataaatgg cttcggttcg cgaattctcg aagaaccatg
     1981 aaaatatttc ttcagcttct tttccgcgca gaatgagcgg tatgccgctt agtttgggca
     2041 taggcttggc aagagcgcga tcgccgacct gtataagtct atcgccgatg ctctgtgcca
     2101 agcgcgctgg gtcgggctcg gagaattcga tatcgtcaaa gagctcgact gtgctatagg
     2161 gattgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaata gtcgatacct
     2281 tgcgaattga gaaggcgtcg ctgttctttg gagatgaaga gttcgagcga attgatacgt
     2341 ggattgacat tttttggcat agctaaggaa tccactatag gtacagcttt aggctgattc
     2401 atgtgttcga agggggagta taaggcagaa cggatagatt tgactcgatc ttcaaaagaa
     2461 agggcttcaa atccggagat cggtacttgt acagaaagtg gggacggagc tggcaggcta
     2521 aaccatggat tgtgagactt ggaggccgca aaaacggcct gtcgtatttt aaccacacat
     2581 tcttccagcg agaaactcgg aaagatggtt atagtggcct cgccgcgaaa tcttctaggt
     2641 gcttcagagc cgtcgacctt ttcggagcta ggagcgctat tggcgctagc ttcatctggg
     2701 gtgttttcat tgccacaggc gctcggatga gaaacatcat cgatactgtc gacatagacg
     2761 gtcaaagaat agacgacttc gtcgatggag cgggcctgct cagcgcgatc gacgatataa
     2821 tacctctctt gccgctggat gttgcgctct acaaggcgcc agtccgcaat ggcttgggct
     2881 actggagatt ctgagtccac aggagaaggt gcggcagcat aagctgctcc tattcgagcc
     2941 gaagcatgca ggttctttga actactctca tttattatcg caatagcctt ctttattgta
     3001 tcgatcatca tcctaacctc attctagttt tgacataggg acctccggaa gagactttgg
     3061 catattcttt atgtcccttg ccgcaagctc cagagccgcc cagttcaaaa tcgttcgata
     3121 ccatgctcac cgaggagagt acatcgggga catagcccga gcatacgatt ggacttgcaa
     3181 cgcgaccagt aaatttgcca tcctttattt ctcggcctac catacagatg agctggatgc
     3241 cccaatttcg gggatcctcc atgcctgcat cgagttgcga gagcttccat ccatgcttaa
     3301 cagaggcgat catgtcttcg agagtggata ttcctggggc aaaataggtg tttgtcatac
     3361 gcgcatatgc cttatgagaa tatgcttcgc gtcttccatt accggttagg ggaagattga
     3421 gcgcgagcgc ggatagggtg tcggatatac cacctttgag aatacctttg tcgatgacgc
     3481 aggtcttgga tgagaagacg ccttcgtcat cgaaaagata agaaccggtc tggtcgacac
     3541 cggctgcgcc atcgtacatt gtgacgagct cagaaccgac ggtcttatcg aggtagtcga
     3601 ccgcgcgagc gcgcttttta aagaacatat cggtctccac gccatggcca aaggcctcgt
     3661 gtgcgagtgt gccggccatg tcggggtcga ggatcacttc gtattcgcca ggttccatgg
     3721 gtacggcatc taggaggtct gcgagctctt tggaaagttc ggggacggag ctttcgagat
     3781 tggccagaat ctctaagcct tttctaccgg atgcggagcg gtaggacatc ttgctgattt
     3841 ctccactacg cgccaagcca aagagataag cttgcgacca cagaaaactc tgcataagtg
     3901 aacgcttagg cgaaacaaaa agcctggaga catcgacaca ttcatagcga gactgagcca
     3961 tgatcacgat cttgccatct gtgagtcttt cgcgcagagc tacaaggcgc tagagaattg
     4021 attcagggtc ggtaacgaat ggatcttctt ggatttctcc gcaatattct tctgtggcgg
     4081 gaatatcggg gatctttggg aattcaatta gcgtcggatc ggttagtaag acctcgaggc
     4141 gggtctggat tatagaagcg gctgatgcag caatcgaact agccttagag gtggcttcag
     4201 agtcggcgcc agataaggat agagatttga tcgcctcata agccaagtcc gaaatcccag
     4261 aaacatcgaa gggcagggtt gaacatgcgt attccacaac cttaccatct cgttgggctc
     4321 gaaaaacaaa gcctcgctga acccacatag gctcgtaggt gcgcgtctcg ccaggcagag
     4381 caacataaga aagaccgcga tcatccgtag cgagcacgct gatataatcg aaatgaggcc
     4441 cgagcagatt gataatctgg tctaatagcg gcttggccgc gagtaaaaaa ggactgtgtg
     4501 atcgtttttg cataatactc ctctatcgag tcaactatag catggattta gatattgtgt
     4561 tgcttttgct ttctagtacc tgtcttactt attcgaacat atagatatgc atctcttgcc
     4621 tggcctgaaa tctgcggcct tatgcggtaa tttggcaata ttttttctct gtgcatgcca
     4681 attctctcaa gcacacgaat agaggcgaca ttttcaacat cgcaagtcgc ccagactagc
     4741 gatacctcag cctgagacaa ggcccaatcg acgatagctc tagcggcttg ggttgcatag
     4801 cctctgcccc aatatttcct attgaggaga tatcctattt ctgccgcatt ttctgctatt
     4861 cgacaggaaa tagcaccgat cgctttgtct gaatctttaa gagtaattat ccaatagtat
     4921 tcgagccctt cattccacag gatttctcgt ctacgcagtg tttcgagaag tggctcgaga
     4981 cttgtgagcc taggccaatc ggcaaatcgt gcaacttctg ggtcgctacc atattcgaaa
     5041 atagctggtg catcactcag acgcggtcgc cttagccaaa tattggtcag ttcgaagctt
     5101 tctggtacga gcattttgtt gctttatgat ataagaatca tattcgaaaa atcgtttgga
     5161 atatgaagct gatcgagtat gttgctgatt tggccacgat aatgagtttg gcgattaaag
     5221 aactggatta ttgccttcca tatctcgcat tcagcttctt cgccttcgct gttttttatc
     5281 gtgagctttt ttgaaaagaa ttcttcttcg actttgctag tgaactcgac aatgagcata
     5341 tctaactttt gccttttcaa tgtgaaatcc tcgaagtttt ggaaaaggct cttgtcgggc
     5401 tcgagtgcga atccaagatc ttcttctata cctttgccgt agttccccag ttccataaag
     5461 gccttgagcc atgagagatc ggtcaaatag atatgttcaa gcaaggttcc tagtgctttt
     5521 aagaaatagc ctttcaaagg agagtttata ggatcctgaa tcatatcgga gattatggct
     5581 atcatgcgag tgttggcatt aagattgtat tgagcataat attgaacgat atctctgatt
     5641 gtgatcatag agacctccgt tggcttgaca ctctatattg caattattat ctttttttga
     5701 aagcgaggca attcatcatg tgtctttata aactaagaag gagtcaaatt ttgattgata
     5761 aaaagctaca atttttatac tatagcaaaa tatacaaaat ataaatttct aactaaatct
     5821 gcatttgtca gaaaatcaaa tctcatgcaa gagaaagggt agtgttatgg catcgttagg
     5881 gaataaagct cgagacattt cgtttttttt ggaagctaaa aaacctgctg aggtgcgtaa
     5941 ggcaatattc aaattggctt tgccggtgat gcttgagttt tttcttatga tgctggttca
     6001 aatgatagac atgatacaag tagggtcgct tggaccatgg gctattgctg caaccggact
     6061 agcagctcag ccaaccatgt taatattctc ggtatttagt gcactggttg tcggaactac
     6121 agcccttgtg gcaagagctt cgggagctgg agatcgtgaa tatgcttgtc gtgtaaccag
     6181 acaatctttt gtaatagtgc tgcttcttgg agtcgttgta accgcagcgg gtattcccgc
     6241 ttccaggact attatccgat taatgggagc aaatgttgat accattaagc cggcaacaat
     6301 ttatatgcaa attgtaattg cagggacagt gtttactgtg ggcaacatgg tactggcagc
     6361 ctctttaaga ggtgtgggcg atgtaataac ccccatgatt tcaaacattg tggcaaatat
     6421 cgtcaatata ttcttgaatt gggtactaat aaatggcaaa ctcggatttc ctcgactaga
     6481 agtagcaggc gcagcaatag ctacatcttt ttccaggtta atagcatttt gcattactat
     6541 gcatgcagta tatgcacgag ataaatatat cagtatatct attcatgatt cataccgctt
     6601 tgaatccaat attatgcgag atgtattgaa cattggcacg ccagcagcaa ttgagcagct
     6661 gattatgagg acgggagcat tattgtttgc acgactgatt gccggatttg gtacgataat
     6721 gtttgctgcc catcagatag caataaatat tgatagtctg ggacttgtac cggcaatggc
     6781 atttcaaatg gctgcgacta ctctcgtggg acagggtata ggtgcaaaaa aacctgaact
     6841 ttcagaagaa gcaacacgac aatcaatgtt tatcagtata ataatcatgt cattagtcgg
     6901 tgttgtttac tttttcttgg gtaaatatat agcaacaatt tttactcgag atgctcaagt
     6961 aattgctctg ggggaaaatg ctttaaaaat tttagcgttc tctcagcctt ttgctgctgt
     7021 ttactttgtt gttgctggtg ctttacgagg tgcaggagat acaaaatatg ccatgtatgc
     7081 cagcgcaatt ggtgtatggg ctattcgcct tgttatggga tatctattag cagatttgtt
     7141 ggatatggcg cttgttggtg catggattgc cgttacccta gatatggcgg ttagaatggt
     7201 gttagtgctt aaaaggtttt ctagtggtgt atttaagaag tatagcaagg agtatagtga
     7261 ggatagtgaa gtgatgatat gaaagcgctt catcagcatt gttagtttta tatggttatc
     7321 taagtctctg ttttgcgtta agcgcatgaa aaacaaagct caaaccaata atcaatgacg
     7381 aggaggatca cgatgcctga attttcaaca gcattttcgg ttcaaaagag cgatcggagg
     7441 ctaacacatc aagagctcgt acgcgctatc cgatttatga tagccgcaga gtatgaagct
     7501 attcaactct atatgcagct tgccgaatcg atcgacaatg atttggctat cgaagtatta
     7561 gaagatattg ccaatgaaga acgggtgcat gcaggcgaat tcttgcgtct attgcaagag
     7621 atcgctcccg atgaatccaa attctataaa gagggggccg aagaagtgga agaggagata
     7681 aaaaaagccc aaaagtagta cacatgactt ttaaggccca gcccaattct cgaaaggcca
     7741 tgcattttaa agttagggct ttcttaatcc tgttcatccc gttcaatccc gtcatcatgt
     7801 caagaattct tatagaatat gctccaaaaa cctgcgtgtg cgctcttctc gcggagaggt
     7861 gaaaaatgtg tccggtggcg cttcttcaac aatttgaccg gcatccataa agattatgcg
     7921 attggcggca gccctggcaa agcccatctc atgcgaaacc accagcatgg tcatgccttc
     7981 atgagcgaga tc
//
LOCUS       DAOSOB010000072         7668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_9538_length_7668_cov_72.761592, whole genome shotgun sequence.
ACCESSION   DAOSOB010000072 DAOSOB010000000
VERSION     DAOSOB010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9538_length_7668_cov_72.761592"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1184)
                     /gene="ilvE"
                     /locus_tag="PLS88_07435"
     CDS             complement(102..1184)
                     /gene="ilvE"
                     /locus_tag="PLS88_07435"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR01123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOU07214.1"
                     /translation="MSRFESMVQSGTIVPTAFSEIDWDKLGFGGIITPFIGGSVASSD
                     GIFEPITIVPNGNISIPPLACCLNYGQELFEGMKANRGPDGRIRLFRIDANAARMAKG
                     AARFMLADPSEALYREAVIETVKANSDYVPPYGKGALYVRPILAGTGMTLNPAPSDTT
                     VFLVTTVPVGMHYKGSSMVSIKVETDFQRAAAKGTGWVKAAGNYAPCFLPTHEAKHQG
                     FDDLLFLNQTGTHVEEVGTANFAMMKHGVLYVADSPSILPGITRDSVMRIARELLDIE
                     VVFAPLELDRVLGLGKFAGEGPADEAFFTGTAAIISPISNINYKGKDYFLGENVGPFA
                     TKLRELLLGIQTGVIPDSFGWVTIVE"
     gene            1692..2969
                     /locus_tag="PLS88_07440"
     CDS             1692..2969
                     /locus_tag="PLS88_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOU07215.1"
                     /translation="MDFFVLIVLVLMYLGIIFMPSKKAWISLGATVVLIVGGAVTPAH
                     AFGSLVNWNILLIYLGSLILAELFIYSRVPAFIAQRIVERSPSIGIAITIILFLTGLL
                     SAFVENVATVLVVAPIMIELGKRGQRNLAPLMIGLAVMANLQGTATLVGDPPSMLFAN
                     FAGYSFNDFFFKLGKPSIFFAIQIGAIVGALYFYFYFRKVESDIKALPPEGIVSWFPL
                     VLLLSMIGGLALFSAISVRGGIHPASGIFVLVLAFVGLAWLVFIKKEKEEAKNLVLHL
                     DWMTLVFLVGIFIVVGVLAESSLLIQLAEQMAQWVKGDILLGFTLIIAISVLISGFVD
                     NVPYIAAMLPVASALAEAMQVQPDLLMFGLLIGSCLGGNLTPFGASANIVAVGLSEKN
                     GSKVSFWNWVKLAGPFTVITTIAASAFIWFVWA"
     gene            3142..6147
                     /gene="purL"
                     /locus_tag="PLS88_07445"
     CDS             3142..6147
                     /gene="purL"
                     /locus_tag="PLS88_07445"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOU07216.1"
                     /translation="MANGENLYRFEVRTRGAGSPRIEALKADVRALGMGILRSARDIA
                     LYFVEGDLTPEELCLLGRFLLSDPVEETFVWREVSRNALHGSTSSPDSVWIVEICRKP
                     GVTDTVANELVRAAREMGILGLNRAATGIRWELEADWLDETTLSKLTRVLLANSVIER
                     WEFGEIEPVFPKGQPFVPAVECYDLRNMDDDSLLSLSFERRLALDLSEMKAIQAWSIA
                     KERKITDVELEMIAQTWSEHCVHKTFKADIEVRNDSNSSAGNSSYPRIIHSIFNTYIK
                     NTTEEIGAPWVKSAFVDNAGIVSFDDQWDISFKVETHNHPSAIEPFGGANTGVGGVIR
                     DIMGVSAKPIAATDILCFGPPDMEEKELPQGTLHPRLVARGVVAGVEDYGNKMGIPTV
                     NGAVHFHPGYAANPLVYCGCVGLAPRGIHRNRPEPGDRVIVLGGRTGRDGIRGATFSS
                     MKMDGSTGEVAGASVQIGDPIVQKRALDVLLAARDAGLYNAVTDCGAGGLSSAVGEMA
                     RALGAEIELSGLGTKYPGLAPWEIWLSEAQERMVLAIPPRHVKAFSELCGIFESEFWD
                     IGAFGSDSELKVRMKGELVLDLDMVFLHQGLPKKLLVAQEPLSKSLQGPLSKSLQEPL
                     KKSLKTNSADLEGQFLKLLCSPEVASRERIVRRYDHEVQGNSVIKPFVGAFCDSPSDA
                     AVLKPREAAGEWGMALSNGFRSDYGELDPYRSAWAAVDEAMRNIVASGGDPRRTAILD
                     NFCMGDPNDPGVMWSLLECARGLREAALSFQTPIISGKDSFYNEYLGADGSRHAVPPS
                     LLVSALAFVPKIERIVTTFLKQPGDSIWLVGDFRPSAIWENEVPDISPKAPNVYGALF
                     DAIQEGEVLAAHDLSEGGLAVALAEMCIGGRLGARIRLPDMNEKRNNPISKAMEQDNI
                     LAVSRLALFGETMGCLLVEVSAGKEKQFAARFASNSSQKSLEQENLAQRLGEVSKEPF
                     LEITDPSLFSVSIKEMFDAWNCASQELLP"
     gene            6144..6905
                     /gene="purQ"
                     /locus_tag="PLS88_07450"
     CDS             6144..6905
                     /gene="purQ"
                     /locus_tag="PLS88_07450"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOU07217.1"
                     /translation="MKAIILKAPGTNRDYDVQSAIEQAGGHADILPISRLRSDARLLR
                     NYGMLVIPGGFSYGDALGAGRLFALDLSRFFAEEVGRFIESGKPVLGICNGFQVLVRA
                     GILPGKQFEPHRFTLAHNENGSFECRWVRLMAPASNSVWTRGIDFIFCPIAHGEGRFS
                     ADSAEAIEKLEKKGCIALQYTDSSGELAHGSYPENPNGSIRDIAGICNPEGNVLGLMP
                     HPENAVFEWQTLALADRTADGARKLFENGLRYAAL"
     gene            6918..>7668
                     /locus_tag="PLS88_07455"
     CDS             6918..>7668
                     /locus_tag="PLS88_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOU07218.1"
                     /translation="MVSEDLVRQLLPIAFRGTGKADGEGKVREWWNLSGNKRLMVTTD
                     RISAFDRVLAAVPFKGQVLNELSAFWFRRTKDIIANHLISLPDPNCSVVEETRPLPIE
                     VVVRGYITGVTSTALWRRYELGERIIYGQRFPEGMRKNEKLPHPVITPTTKGGPGSHD
                     ERLEPREVVEKGYLDAAIWNDVQDAAFALFSRGTDCAKRAGLILVDTKYEFGIAPDGS
                     LMLIDEVHTPDSSRFWLASSYEERFEAGLEPE"
BASE COUNT         1874 a         1685 c         2041 g         2068 t
ORIGIN      
        1 caagcagcat tctcttgcct attgggccat tttgcaatct tttctctacc gggctctctt
       61 ggcttttcgc ttcttttgct tctttctctt ttgtccgttt cttattcgac tatggtcacc
      121 cacccaaatg agtcgggtat tacaccggtc tgaattccga gtagaagctc gcgtagcttg
      181 gtcgcaaatg gcccgacatt ctctccaagg aaatagtctt ttcctttata gttgatattg
      241 gagatggggc ttatgatagc cgcagttccc gtaaagaaag cttcatccgc gggcccttcg
      301 ccagcaaact tcccaagtcc taggacacga tcgagctcga gaggtgcaaa gacgacctcg
      361 atatcgagca attctcttgc aatgcgcatt actgaatcgc gagttatgcc aggaagaata
      421 gaaggagagt ccgcaacata tagtacgcca tgcttcatca tagcaaaatt ggcagtgcca
      481 acttcttcga catgggtccc agtctggttg agaaaaagca aatcgtcgaa gccttggtgc
      541 tttgcctcgt gagtgggaag aaaacaggga gcatagtttc ctgcggcttt tacccagcct
      601 gtgccctttg ccgcagcccg ctgaaagtct gtttcaactt ttatgctgac catagagctt
      661 cccttgtaat gcatgccgac tggaacagtg gtgacaagga acaccgttgt atccgatgga
      721 gctgggttga gggtcatacc ggttcctgcc aggattggtc ttacgtatag ggctcctttg
      781 ccataaggag gcacatagtc ggaatttgct ttgacagttt cgattacagc ttcgcgatac
      841 agcgcttcgg atggatctgc aagcatgaaa cgcgcggctc cttttgccat tcgcgctgca
      901 tttgcatcga tacgaaagag tcttatccga ccatctggac cgcgattggc cttcattcct
      961 tcgaagagtt cctgcccata gttaaggcag catgcaaggg gaggaataga tatgttgcca
     1021 ttaggtacga tcgttatagg ttcgaatatc ccatcggatg aagctacact tcctccgatg
     1081 aatggtgtga ttattccccc aaaacccagc ttatcccaat cgatttcgga aaaggccgtg
     1141 gggacgatgg tgccagattg gaccattgat tcaaatcgac tcataagaac ctcccgaaaa
     1201 attttcgaat aataacgcac aaagggtaat aaagacaata ggagaactca ataaaatgca
     1261 aaatgacctt atatatatgc aatgctttta tgtggtactg atcgattgca tggcagcttc
     1321 ctggaaacct gaagagttga tcgccacgca tttgaagtca catttagagg cgcacttaaa
     1381 agcgcataat tttcttgtgc aaaggtttct catacaaaac agatcagaac tgcaacaatc
     1441 ttctattgac tgacattttc tgaatggagt atcttaaaag tctagcgggg agtagctttc
     1501 tgcgtttaga tagcagatgc gaaaatcgtc aagacggagc tttgctcccg gttttcgcag
     1561 atccaaaatg attgagcaag accgcacggt gtcatggccg tgcggtcttt tttttgactg
     1621 tacagtgacc aaaaacgcct aagaagttgg ttgtaggcga aatgccattg ggcttttcaa
     1681 aggaggatac cttggacttt tttgtgctca ttgtgctcgt gttgatgtat ctgggaatca
     1741 tttttatgcc ttcaaaaaag gcatggattt ctttgggtgc gactgtcgtg ctcatcgtgg
     1801 gaggtgcagt tactccagca catgcctttg gttcgcttgt aaactggaat attctgctaa
     1861 tctatttggg gagccttata cttgccgagc ttttcatata ttcgagggtt cctgctttta
     1921 tcgcccagcg aattgttgag cgctctcctt cgataggcat tgccataact atcattcttt
     1981 ttttgacagg gttattgtct gcattcgtcg agaacgttgc tacggtgctt gtagtggcgc
     2041 ctatcatgat tgagctggga aagcgaggcc agagaaatct tgctccgctc atgatcggac
     2101 ttgcggttat ggcgaatttg cagggcaccg ctactttggt cggcgatcct ccttccatgc
     2161 tctttgccaa ctttgcaggc tactcattca acgatttctt tttcaagctt gggaagcctt
     2221 cgattttctt tgccatccag attggcgcaa tcgtaggagc gctttatttc tatttctatt
     2281 ttagaaaagt tgagagcgat atcaaagcct tgccgccaga gggcattgtg tcatggtttc
     2341 cattggttct gcttctgtct atgatcggag gccttgctct gttttctgct atatcagtcc
     2401 gaggtgggat ccatcccgca agcggaattt tcgttcttgt gcttgctttt gttggacttg
     2461 cgtggcttgt ctttataaag aaagaaaaag aagaagcgaa aaacctcgtt ctccatctag
     2521 actggatgac actggttttc ctagttggca ttttcatcgt agtgggagtt ttggccgaat
     2581 cgagtcttct aatacaactt gcagaacaga tggcccaatg ggttaaaggt gacatcttat
     2641 tgggttttac gctgattata gccatatctg tgcttatttc gggatttgtg gacaatgtgc
     2701 cgtatattgc ggcgatgctt cctgtagcga gcgcgcttgc ggaggcaatg caagttcagc
     2761 cagatttgtt gatgtttggc cttctcattg gttcttgcct tggcggaaat ctgacaccct
     2821 ttggggcttc cgctaacatc gtcgcagtag gcctttcgga aaaaaatgga tcgaaggtta
     2881 gtttctggaa ctgggtcaag cttgcgggtc cctttaccgt aataacgaca attgcggcat
     2941 cggcatttat ttggtttgtg tgggcttgaa tctattttat aatattttat agggccataa
     3001 gattaggtca aatgcgcatg ataagctcgg ctgctagcta agatttatgg gcaagcagct
     3061 gtcggtcaaa attcaaaagg cgccattggc tgagatttat gggcagcttg acaatctatc
     3121 catatcgcga tagatagtat tatggctaac ggagaaaatc tttataggtt cgaggttcgg
     3181 accaggggag ccggaagccc gcgcatagaa gcgttaaaag cggatgtacg cgcgctaggg
     3241 atggggatac tgcgcagtgc gcgagacatc gcgctctact ttgtggaagg tgatttaacg
     3301 cccgaggagc tttgtcttct cgggcgtttt cttttatccg atccggtgga ggagacattt
     3361 gtatggcgag aagtgtcgcg aaatgcatta catggttcaa cctcaagtcc ggattcggtc
     3421 tggatcgtag agatttgccg aaagcccgga gttacggata ctgtcgccaa cgagcttgtc
     3481 agagcggcgc gggagatggg tattttaggt ctcaataggg cagcgacagg catccgttgg
     3541 gagctcgagg cagactggct cgatgaaacc acactatcta agctgaccag agtgcttcta
     3601 gcgaattcgg tgatagagcg ttgggagttt ggtgaaatcg aacctgtttt tccaaagggg
     3661 cagccttttg ttccagcagt agagtgctac gatcttagaa acatggatga tgattctctg
     3721 ctctcgctat ctttcgaaag gcgactggca cttgatcttt ccgagatgaa ggccattcag
     3781 gcttggtcta tcgcaaagga acgtaagatt actgatgtag agcttgaaat gatcgctcag
     3841 acatggagcg agcattgcgt gcacaagacc ttcaaagccg atatcgaggt aagaaacgat
     3901 tcaaattcaa gcgctggaaa ttcatcatat ccaagaatta tacatagtat tttcaacacc
     3961 tatatcaaaa acactaccga ggaaattgga gctccgtggg taaagtccgc ctttgtagac
     4021 aatgctggca ttgtgagttt cgatgaccaa tgggacattt cattcaaggt ggaaacacat
     4081 aatcatcctt ccgcaatcga gccttttggg ggcgctaata cgggagtagg aggcgttatt
     4141 cgcgatatta tgggcgtctc cgcaaagcct atagcagcga ccgacattct atgctttggg
     4201 ccacccgaca tggaggaaaa agaattgcct caagggacgc tgcatccacg ccttgtagcg
     4261 cgaggcgttg ttgcgggtgt cgaggattat ggcaacaaga tgggtattcc cactgtcaac
     4321 ggggcggttc attttcatcc tggctatgca gcaaatccgc tcgtatactg tggctgtgtg
     4381 gggcttgcac ctcgtggcat tcatcgaaat agaccagagc cgggcgatcg tgtgatagtt
     4441 ctgggagggc gaacaggccg agatggcatt cgcggagcca ctttttcttc gatgaagatg
     4501 gatggttcta ctggagaagt tgccggcgca tcggtacaaa taggagatcc tattgtccaa
     4561 aaaagagccc tagatgtgct tctagcggcg cgcgatgcag gactatacaa tgcggtgacg
     4621 gattgtgggg caggaggact ttcttctgcg gttggggaaa tggcacgagc gctcggagcg
     4681 gagatcgagc tttctggtct tggcaccaaa tatcccgggc tcgctccctg ggagatctgg
     4741 ttgtccgagg ctcaagaacg tatggtctta gcaatacctc ctcgacatgt caaggctttt
     4801 tcggagctat gcggtatttt tgaatccgaa ttttgggata ttggggcatt tggaagcgat
     4861 tcagagttga aagtgaggat gaagggcgag ctcgtactcg atctggacat ggtattttta
     4921 catcaaggct tacctaaaaa gctccttgtg gcgcaagaac cactatcgaa atcgttacaa
     4981 ggaccactgt cgaaatcgct acaagaaccg ctaaaaaaat cgcttaagac aaattcagcc
     5041 gatctcgaag gccagtttct aaagctgctt tgtagcccgg aagttgcctc gcgcgagaga
     5101 atagtgcgcc gctatgacca tgaagtgcaa ggcaatagcg ttatcaagcc cttcgtaggt
     5161 gctttctgcg attcgccttc ggatgcggcg gttctgaagc ctcgagaggc agcaggagaa
     5221 tggggcatgg cgctttccaa tggctttagg tcggactatg gggaactcga tccttatcgg
     5281 agtgcatggg ccgcagtgga cgaagccatg cgaaacatag tggcaagcgg aggagatcca
     5341 cggcggactg caattctcga taatttctgt atgggagatc caaacgatcc tggtgtaatg
     5401 tggtcgcttt tggagtgtgc acggggccta agagaagctg ccttgtcctt tcaaactcca
     5461 atcatctctg gtaaagattc cttttacaat gaatatctgg gcgctgacgg aagcaggcat
     5521 gccgttccgc cgtcgctgct tgtatcggca ttggcctttg tgcccaaaat cgagcgaata
     5581 gtgactactt ttcttaagca gccaggcgat tcaatatggc ttgtagggga ttttaggcca
     5641 tctgcaatat gggaaaacga agtgcccgac ataagcccaa aagctcctaa tgtctatggt
     5701 gccttgttcg atgcgattca agaaggggag gtgctcgccg cacatgatct ctccgaagga
     5761 ggccttgccg tagctcttgc ggagatgtgc attgggggaa gattaggcgc tcggatcagg
     5821 ctgcccgata tgaatgaaaa gagaaataat cccataagca aggccatgga acaagataat
     5881 attcttgcag tatcaaggct tgctctcttt ggtgagacga tgggctgtct tttggtcgag
     5941 gtaagtgctg gaaaagaaaa gcagtttgcg gcgcgattcg cttcgaattc ttcacagaag
     6001 agcttagaac aagagaatct agctcagagg cttggcgaag tatcgaagga accctttctg
     6061 gaaatcaccg atccatctct tttttcggtt tcaataaaag aaatgttcga cgcttggaat
     6121 tgcgccagcc aggagttgct tccatgaaag cgattattct caaggctcca ggaaccaatc
     6181 gagattacga tgtgcaatct gccatagagc aagccggagg tcatgctgat atcttgccaa
     6241 tttcccgtct tcgtagcgat gcaaggcttt tgcgaaacta tggcatgctc gtcattccgg
     6301 gaggcttttc ctatggggat gccttaggag caggaaggct gttcgctctc gacctttcac
     6361 gcttctttgc ggaagaagtg ggacgcttta tagaatctgg caagccagta ctaggtattt
     6421 gcaatggttt tcaggtattg gtgagagcag gaatcctgcc gggaaaacaa tttgaacctc
     6481 atagattcac tcttgcgcac aatgaaaatg ggagtttcga gtgcagatgg gtgcgtctta
     6541 tggcaccagc ttccaattct gtttggacaa gagggatcga tttcattttc tgcccgatag
     6601 cccatggcga agggcgcttc agcgcagact cggctgaagc tattgaaaag ttagaaaaaa
     6661 aaggctgtat cgctctccaa tatacagatt cttcgggtga gctcgcccat ggcagttacc
     6721 ccgagaatcc caatggatcg ataagagata ttgcgggcat ctgcaatcca gaaggcaatg
     6781 tgctaggact aatgcctcat cccgagaatg cggttttcga gtggcagact ctggctctgg
     6841 ctgaccgcac agcggatggc gcgcgcaagc tctttgaaaa tggattgcgg tatgcggctt
     6901 tgtgagaagg aggcagtatg gtttcggagg atctagtcag gcagcttctt ccaatcgctt
     6961 ttcgagggac aggcaaagcc gatggagagg gcaaggtcag ggaatggtgg aatctgtcgg
     7021 gcaataagag gcttatggta accacggata gaatctctgc ttttgatagg gtgcttgcag
     7081 ccgtgccttt caaggggcag gtcctaaatg agctttcagc cttctggttt agaagaacta
     7141 aagatatcat cgccaatcac ttgatctctt tgcccgatcc taattgctcc gtggtcgagg
     7201 agacgaggcc gttaccgata gaagttgtag ttcgaggtta cataactggt gtcacttcca
     7261 ctgctctctg gaggcgatat gagctaggcg agcgcataat ctatgggcag cgttttcccg
     7321 aaggtatgcg caagaacgag aagcttccgc atccagtgat cacacccaca acaaagggcg
     7381 gtcctggcag ccacgatgaa aggctcgaac ctcgagaagt agtggaaaaa ggctacttag
     7441 atgcagccat atggaatgat gtccaagatg cggcttttgc tcttttttct aggggaacgg
     7501 attgtgccaa gcgcgctggc cttatcttag tggataccaa atatgaattc ggcattgctc
     7561 ctgacggttc gctcatgctg atcgacgaag ttcacacgcc tgattcatcg cgcttctggc
     7621 ttgcttcgag ctatgaggaa cgattcgaag cggggctcga gccagaaa
//
LOCUS       DAOSOB010000073         7660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_9557_length_7660_cov_72.166864, whole genome shotgun sequence.
ACCESSION   DAOSOB010000073 DAOSOB010000000
VERSION     DAOSOB010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9557_length_7660_cov_72.166864"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..75)
                     /locus_tag="PLS88_07460"
     tRNA            complement(3..75)
                     /locus_tag="PLS88_07460"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Met,seq:cat)
     gene            complement(242..925)
                     /locus_tag="PLS88_07465"
     CDS             complement(242..925)
                     /locus_tag="PLS88_07465"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HOU07219.1"
                     /translation="MEIGNAKQCPLCGTPNPRACEEKNLVTDSIDDQTSSKEEICSAE
                     KRTTVWEVLSVTLAIAILVLGSINLFESRRFSWSLYAIVSIFFIWVELTAFFMLRKKA
                     VLRILMSVLAPPFLFITIGYISGGQRWALGLAVPIIVIVESIIGLVYLLFEKSKQKGL
                     NLIAYMTVAIALLCFCLEVFIDLFANGVVVLKWSPICAIALLSIAVFFLYLHFRVFKN
                     TDLHRIFHF"
     gene            complement(971..2347)
                     /locus_tag="PLS88_07470"
     CDS             complement(971..2347)
                     /locus_tag="PLS88_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07220.1"
                     /translation="MNRYGQSTIDRRFVHTRREDNAGEWYALDNAAIIMPAVTDDVST
                     SLFRFEAELDAPIDPEILKKALDLTVSRMPYFNVALRRGFFWYYFEPCKTLPPLFPDS
                     PIPCLRWNINRRGTRMFRILWEQNKVAGEFSHALTDGTGGIAFFKSLLANYLILKGVD
                     PGVQLGEGEWRDIIVSFGDMSSSATSEEFEDAYQRYFPNGLPFPDLRPHAFHLSKTCL
                     AKGQYRVISGKLLTSEVIAEAHRRNVSLTELLTAVYLDALQHIWHAMKPHPREPYISI
                     EIPINLRQLFPSCTFRNFSLFVLISEDMRLGMRDFDELVSRTHYQMKLEYDRMSIARH
                     LCRNAGSARNLAVRLVPLRVKDFFAKVFFSKFGESMLSGFISNIGQLKMPKAFASHIL
                     SFGFIPTPRMSTMTNASMLSWGNDLIINFGSLAQSRDLERLFFRRLRSIGLHVSVSCR
                     SDNEIQGD"
     gene            complement(2355..3050)
                     /locus_tag="PLS88_07475"
     CDS             complement(2355..3050)
                     /locus_tag="PLS88_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOU07221.1"
                     /translation="MVATLINAGAIILGSIIGILIRKGIKEEYRKVVFTAAGITSLTI
                     GIQMVLKTSHILSFALALMAGGLLGTMLDLEGAIERLGESLKRRFARKSEDTFAAGFL
                     NASVLFCAGAMAILGSFNAGTVGDYSLLLTKSVLDGFVSIIFAGAMGIGVAFAALSVL
                     VYQGALTLLSEYIKPYVSDLMLSELTAIGGALVIMIALGLLEIKSFKTGNFLPAMVVI
                     IILALAMPFISFL"
     gene            3108..3518
                     /locus_tag="PLS88_07480"
     CDS             3108..3518
                     /locus_tag="PLS88_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07222.1"
                     /translation="MHRSLLRYVALFALSLVGLLVFSCASSPTPVPEDASAQKIIQLA
                     QERYDVYDLNGAKYYYQVLLERYGSDPNYMLNAKYEIAFIEYKQGNKDKAIAGFREII
                     ERYNEPDASSLSPTWKVLSEMMIEKLESNKNTKS"
     gene            complement(3570..5555)
                     /locus_tag="PLS88_07485"
     CDS             complement(3570..5555)
                     /locus_tag="PLS88_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOU07223.1"
                     /translation="MPFLQLSGIRLSFGARDLILNASLNLQDGSRAALTGPNGAGKST
                     LMKIAAGLVKPDAGEVVATKDARIAYLPQTGIQFESGTVHEIAESAFSFYHNLLKEQE
                     QIGRSLDSSILSEKETERLLAKHHEITERLEQAEYWTREERISEVLSGLDFKIKDFGR
                     SVSELSGGWQMRLALAKILLEEPDIMLLDEPTNYLDLEARTWLEDFLKAYPGAVLVVS
                     HDRFFLDVTVKEVYELFNGTLTRYVGTYSQYEIRRSQELEALFEAWEHQQEERQRIED
                     FIRRFRYKDSKAAQVQSRIKMLEKMTPIEIPEGMKRIHFSFPPAPRSARVVLRMRELS
                     KSYGELMVIEPFSLEVERGQKLALIGPNGAGKSTLMRIIAKEDNPTGGEAILGANVLV
                     GYFSQESAEIMESTATVEEEAESVCPPEMLPKIRNLLGAFLFRDDDIEKPISVLSGGE
                     RSRLALLKLLLKPANLLVLDEPTNHLDLTSKDILLEALSKFEGTVIFVSHDRQFLDEL
                     PDRVLELSCGNVPRLYYGNYAYYLKKKAEKENMRLEDIKEDIRKPPRQRRQSDNTSVS
                     SVANQTSVPNRDWEEEKARRARLRKLKRREEEISTRIEEIQTEKERLQGELAQPRNYI
                     NGEKTRRILTELEVLDRETEALNEEWLEIAQSLAEEA"
     gene            complement(5568..6971)
                     /locus_tag="PLS88_07490"
     CDS             complement(5568..6971)
                     /locus_tag="PLS88_07490"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU07224.1"
                     /translation="MRRERGIIKGILSVFGLALILFCILLAGCARLEGWGLVIWSVKG
                     TAAKAGTIVPVYLKSNISKTYIIGLPDDSKTKIEVPFATIEFFTSKKAAEKRAREFAP
                     YALYYLSAGRDGLPVRDKPSVTGRRVYRLRLGESVKVLNRVEGEAVFTGGKALPGEWY
                     FVQATDGTRGYVFSNTMILYEEKEGISVPVLTTAPTPSASLLDMIYAKPWRPAYYQLM
                     LDEDTVDPDLFLLQYGLFADAKNSQVRIELPGYSKAFDFSSVSQMGDWLVFEGSNLRI
                     KFENESTIVADWSGLEPVLPEEGWIFGGQAARFVHLDTSIPVIISGESSRRDSELKIF
                     FQRVSQLQTDGSSSVTFLSDHAGILTIDLKGNFEWSHIDQLPAGFAPEISPGSDTNAL
                     KGKIRFGLHLGAPLSSYWMGGFSLLMGKESQRYDFVYRFENEQLIIAKAIQVSLRGTT
                     ESIDSRFSPVNYYLINK"
     gene            7059..>7660
                     /locus_tag="PLS88_07495"
     CDS             7059..>7660
                     /locus_tag="PLS88_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOU07225.1"
                     /translation="MSETLSQKSQEIKENSGNPPSIHAAIEIGSTGIRLIIAEIDSKG
                     DLKILDRASKQSRIGRDVFTMGNISRDGMRETIAILDSFSELLAGYGLRPADIQVIGT
                     SALREASNRDTFIDRISLQTSYHVRIIEDIEENHLMYLAVKKALEDERSYLGRSNSMI
                     LEVGGGTTELMLLKKGQMVSSHSLSIGTLRIDEQIRESAQ"
BASE COUNT         2157 a         1802 c         1627 g         2074 t
ORIGIN      
        1 tctagcaggg gaaggactcg aacctccgac ctccgggtta tgagcccgac gagctgccaa
       61 ctgctctacc ctgcggcgca agaaaaattt acagtaaatc gcagaatatg tcaagggata
      121 gaaatatttt ctgatacaat accaggtttt cacaatcagg ataagaagat aggagtctag
      181 ttcgggttaa attatgttag cctaatttaa tctcataggc ccttctcgcc agattgaagc
      241 actagaaatg aaagatgcga tgcaaatccg tattcttgaa aacacgaaag tgaagatata
      301 agaagaagac cgcaatggat aaaagagcaa tagcacagat aggagaccat ttaagcacaa
      361 cgactccgtt cgcaaataga tcgataaaca cctcgaggca aaaacagagc agcgctatgg
      421 ccactgtcat gtaagcaatc agattgagtc ccttctgctt acttttttca aataggaggt
      481 aaaccaggcc aataatagat tccacaatta ctataattgg tacagctaga ccgagcgccc
      541 atcgctgacc acctgaaata tacccaattg ttatgaacaa gaatggcggc gcaagaacgc
      601 tcattaagat ccgaagcaca gccttctttc tcaacataaa aaacgccgtc aattccaccc
      661 atataaaaaa aatcgataca atggcgtaga gggaccacga gaatcgcctc gattcgaaaa
      721 gattaatgga gccgagcacc aatatggcga tagccaatgt cacagagagt acttcccaga
      781 cagtcgtccg cttttctgcg gagcagattt cttctttgga acttgtctgg tcgtctatcg
      841 agtcggtaac tagatttttc tcttcacacg ccctcgggtt tggggttccg cagagcgggc
      901 actgtttagc atttcctatt tccaccccac aatcagggca atacgacatg attcaccccc
      961 gttcttgctt tcaatctcct tgaatttcat tgtcagatcg acacgaaaca ctgacatgta
     1021 atccgatact tcgcaatcgt ctaaagaata gccgttcgag gtcgcgcgat tgcgccaagc
     1081 ttccaaaatt gataataagg tcgttgcccc aactgagcat tgaagcgttt gtcatggtac
     1141 tcattcttgg agtgggtatg aatccaaagc tcaatatgtg agaagcaaaa gctttgggca
     1201 ttttcagttg gcctatgttg gaaatgaaac cagaaagcat gctctcgcca aatttggaga
     1261 aaaagacttt agcaaaaaaa tctttaactc taagaggaac caatcggaca gccagatttc
     1321 tcgcacttcc agcattacgg cacaagtgtc ttgctatgct cattcgatca tattcgagct
     1381 tcatctggta atgagtccgc gataccagct catcgaaatc tctcatacct agccgcatat
     1441 cttcgctgat aagcacaaaa agagaaaaat tgcgaaaggt acacgaagga aaaagctgtc
     1501 gcagattgat gggtatctct attgatatat aaggctcacg aggatggggc ttcattgcgt
     1561 gccatatatg ctgcaaggca tcgagataga cagccgttag gagttcagtc aggctgacat
     1621 ttctccgatg agcctctgcg ataacttcgc tggtcagcaa cttccccgaa attacacgat
     1681 actggccttt ggccaagcag gttttactca aatgaaaagc atgcggcctt aagtccggga
     1741 atggtaatcc attgggaaaa taccgctgat aagcatcttc gaattcttcg cttgtcgctg
     1801 agctgctcat atcgccaaag gagacaatta tgtctctcca ctcgccttcc cctagttgca
     1861 caccagggtc gacacccttc aatataagat aattcgcaag gagagactta aaaaaagcta
     1921 tacctcctgt gccatcggtc aaggcatgcg agaactctcc tgcaacctta ttctgctccc
     1981 aaagaattcg aaacatgcgc gttccgcggc gattgatgtt ccatcttaga cagggaatag
     2041 gagaatccgg aaagagtggc ggcaaggttt tgcatggctc aaaataatac cagaaaaatc
     2101 ctcgtcgaag tgcaacattg aaatagggca tgcggctcac tgtaaggtca agtgcctttt
     2161 tcaatatttc tggatctatg ggcgcatcga gctcagcttc gaaccgaaaa agactcgtgc
     2221 tgacatcatc ggtgaccgct ggcattatta ttgcagcatt atccagtgca taccattcgc
     2281 cagcattatc ttcgcgccga gtatggacga atctacggtc tatggtactc tggccatacc
     2341 tgttcatgtc ttaattatag aaatgatata aatggcattg caagggcaag aattataatg
     2401 acaaccattg ccggaagaaa attacctgtt ttgaagctct taatctctaa gagccctaaa
     2461 gctatcatta tgacgagtgc tcctccgata gccgtgagct cgctcagcat taggtcactg
     2521 acataaggct ttatgtactc ggacagcaaa gtaagagccc cttgatacac aagaacggac
     2581 agggctgcaa atgccactcc gatgcccatt gcacctgcga atattatcga gacaaagcca
     2641 tcgagcacgc tctttgtcaa tagaagcgaa taatccccta cggttccagc gttaaaagaa
     2701 cccaatatgg ccatggctcc tgcacagaat aaaaccgaag cattgagaaa accagctgca
     2761 aaggtgtctt ctgatttacg agcgaatcgt cttttgagac tctctccaag cctctctatt
     2821 gcaccttcga ggtcgagcat tgtacccaag agtccccctg ccatcaacgc aagggcaaaa
     2881 gaaaggatat ggctggtttt tagcaccatt tgaataccaa ttgtaagcga tgtaatccct
     2941 gccgcagtaa atacgacctt tcgatattcc tcttttatac cttttcgtat aagaatacca
     3001 atgatcgaac ccagaataat ggcccctgca ttgattaatg ttgcaaccat gcttgtcttg
     3061 acctcgttca gcgagtatag cataattgcg acttgtgagg aaattacatg catagatctc
     3121 ttttacgcta tgtagcctta tttgccctct cacttgtcgg gcttctggtg ttttcctgtg
     3181 cctcttcgcc tacgccagtt cctgaagatg caagcgctca gaagatcatc cagctagccc
     3241 aggaacgata tgatgtctat gatctaaatg gtgctaagta ttattatcaa gtactattgg
     3301 agagatatgg cagcgatcct aactacatgc tcaatgcaaa atatgagata gcctttattg
     3361 aatacaaaca aggcaataag gataaggcga tcgctggctt tagggaaata attgaacgct
     3421 ataatgagcc tgatgcatcg agcttatccc caacatggaa agtactttcc gagatgatga
     3481 tcgaaaagct cgagagcaat aagaatacta aatcctaaat gctttaggcc gcgctttttt
     3541 aatttcaagg agcctatttt aagtagtcct caagcttctt cggctaaaga ctgcgcaatt
     3601 tcgagccatt cttcattgag agcttcggtt tctcgatcga ggacttctaa ctcggtcaga
     3661 atgcgccgcg ttttttctcc atttatgtag tttcgaggct gagccaattc tccctgaagc
     3721 ctttcttttt ctgtctggat ttcttcaatg cgagtagaaa tctcctcttc cctacgttta
     3781 agcttgcgta atcgagccct gcgcgctttt tcttcttccc aatctctgtt tggaactgaa
     3841 gtctggtttg cgacagaaga gactgaggta ttatcacttt gccggcgctg tctaggaggc
     3901 tttcgaatat cttcttttat atcctccaac ctcatattct ctttttcagc tttctttttt
     3961 agataatagg catagttccc ataatagagg cgcggcacat ttccacagga gagttccaga
     4021 actcgatccg gaagctcatc gaggaattga cgatcgtgag agacaaaaat gactgtgcct
     4081 tcgaatttac tcaaggcttc gagcaaaatg tctttgctcg tcaaatccaa atgattggtc
     4141 ggttcatcga gcactaggag gtttgcaggt tttagtagaa gcttgagaag agctaaccgc
     4201 gagcgctcac ctccagataa cactgagatt ggtttttcta tatcatcatc gcggaaaaga
     4261 aaggctccaa gcagattacg aatctttgga agcatctcgg gtggacagac agattcggct
     4321 tcttcttcta ctgttgcggt cgattccatt atctctgcgg attcctgtga aaaatagcct
     4381 acgaggacat ttgctcctaa gatagcttct ccgcctgttg gattatcttc ctttgcaatg
     4441 atgcgcatga gagtagattt ccctgccccg tttggaccta taagagccaa tttttgtcca
     4501 cgctctactt cgagagaaaa tggttctatt accatgagtt ctccataaga tttggagagc
     4561 tcgcgcattc gtagaaccac acgagcagac cttggagctg gcggaaaaga aaagtgaatt
     4621 cgcttcatgc cctctgggat ctcgataggc gtcatttttt ccagcatttt aatgcgactc
     4681 tggacctgag cagccttgga atccttgtat ctaaagcgcc gtataaaatc ttcgatcctt
     4741 tgcctttctt cttgctgatg ttcccaagct tcgaagagtg cctctagctc ttgggatctg
     4801 cgtatttcat actggctata ggtccccaca tagcgcgtaa gagtaccatt gaatagctca
     4861 tatacttctt ttactgtaac atcgagaaag aatctgtcat gcgaaaccac tagcactgca
     4921 ccagggtagg ctttaagaaa atcttctagc catgtgcgtg cctctaggtc gaggtaattg
     4981 gttggttcgt ccaataacat tatatctggc tcttcgagca atatcttcgc cagcgcaagt
     5041 cgcatctgcc agccaccaga tagctcggac acagagcgtc caaaatcctt tatcttgaaa
     5101 tctagaccac ttaaaacttc ggatatcctc tcctcgcggg tccagtattc tgcctgctca
     5161 agcctttcag ttatttcatg atgctttgca agaaggcgtt ctgtttcttt ttcactcaaa
     5221 atagaggaat ccaatgatct accaatttgt tcctgttcct ttagaagatt atgatagaat
     5281 gaaaatgcgc tctctgcgat ctcatgaaca gttcctgatt cgaattgaat tcctgtctgc
     5341 ggaaggtagg ctattcgagc gtcctttgtt gcgacgactt cgcctgcatc tggtttgaca
     5401 agtcccgcag ctattttcat aagtgtcgat ttgccagcgc cattgggccc agtcaatgct
     5461 gcgcgagatc catcctgcaa attcaagctt gcattcaata taagatctcg ggcgccgaat
     5521 gaaagcctaa ttccgctcaa ctgcagaaag ggcatgttat ctcaacctta tttatttata
     5581 aggtaataat ttacaggaga gaatctcgaa tcgatactct ccgtagttcc tcttaggctc
     5641 acctgaatcg ctttggcaat gataagctgt tcattctcga agcgatatac aaaatcatac
     5701 ctctgggatt ctttacccat aagaagcgaa aaaccaccca tccaatagga tgaaagagga
     5761 gcccctaaat gtagcccaaa ccgaatttta ccttttagag cattggtatc ggatcctgga
     5821 gatatttcag gggcaaaacc agcaggcagt tgatcgatat ggctccattc aaagttccct
     5881 ttaaggtcta tagttaaaat ccccgcatgg tcagaaagaa aggtcactga tgagctacca
     5941 tctgtttgaa gctgtgagac tctctggaaa aagatcttca attccgaatc acggcgtgaa
     6001 ctctctcctg agataataac tggaatcgat gtatctaggt gtacgaaacg agctgcttgt
     6061 cctccaaaaa tccagccttc ttcagggagc acaggctcta gaccactcca atccgctaca
     6121 attgtcgatt cattttcgaa tttgattcta agattcgatc cctcgaacac gagccaatct
     6181 cccatctgac tgactgaact gaaatcaaag gccttcgaat agccaggtag ttctattctg
     6241 acttgagaat tctttgcgtc cgcaaaaagc ccatattgga gcaagaaaag atctggatcg
     6301 acagtgtctt catctagcat gagctgataa taagctggac gccacggttt tgcgtatatc
     6361 atatcgagca atgaagcgct gggggttggg gccgttgtta atacaggcac gcttatgcct
     6421 tctttttcct catatagaat catggtattg ctgaatacat aaccccgcgt gccatcggta
     6481 gcctgtacaa aataccactc tccaggaagc gcttttccac ctgtaaatac agcttcgcct
     6541 tctactctgt taagaacttt caccgattcg ccaagcctta gccgatagac tctccggcca
     6601 gtcacgcttg gcttgtcgcg cacaggaagg ccatctcgcc cagcgcttag ataatagaga
     6661 gcataaggcg caaattccct ggccctcttt tctgccgctt ttttcgaagt aaaaaactcg
     6721 atcgtagcaa aaggcacttc tatttttgtt ttgctgtcgt cgggcaaacc aattatatag
     6781 gttttgctta tgttcgattt taggtaaaca ggaacgatgg tgcccgcctt ggccgctgtt
     6841 ccttttacag accaaatgac caaaccccat ccttctaatc tcgcgcagcc agctagcaaa
     6901 atgcaaaata gaatgagagc aaggccaaaa accgacaaaa tccctttaat gatgccgcgc
     6961 tctcttctca ttttaagatt catacctcaa gagtgtacaa tgcatggaaa attctggcta
     7021 gtatttttag tcagcatgaa gatgctataa taaattatat gtcagagaca ctctctcaga
     7081 aatcgcaaga aatcaaggaa aatagcggga atccgcctag tatccatgct gctatagaaa
     7141 tagggtccac cggcatacgt ctcataatcg ctgaaatcga ttcgaaagga gacttaaaga
     7201 tactcgatag agcctccaaa caatcccgca ttggtcgcga tgtattcact atgggcaata
     7261 tttctcgaga tggtatgaga gaaacaattg ctatacttga ctcatttagt gagcttcttg
     7321 caggttatgg tttacgccct gcagatatcc aggtgatagg aacgagcgcg ctccgagagg
     7381 cctcgaatag agatactttt atagatcgca taagcctgca gacaagttat catgttcgga
     7441 tcatcgagga catagaagaa aatcatctta tgtacctggc ggtaaaaaag gcactcgagg
     7501 acgaaaggtc ctatcttggt cgctcgaatt cgatgattct agaggtaggg ggtggcacta
     7561 ctgagctcat gcttctaaaa aaggggcaaa tggtctccag ccatagcctt agcataggca
     7621 ctcttagaat agatgaacag atccgcgaat cagcacagca
//
LOCUS       DAOSOB010000074         7283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_10252_length_7283_cov_95.280576, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000074 DAOSOB010000000
VERSION     DAOSOB010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10252_length_7283_cov_95.280576"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1147)
                     /gene="rhaS"
                     /locus_tag="PLS88_07500"
     CDS             complement(134..1147)
                     /gene="rhaS"
                     /locus_tag="PLS88_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOU07226.1"
                     /translation="MKKTVSRVALILFVLALIIPSAVFAAEKPKFAIVFKNTGNPYGE
                     KQLEGFRVGIEEQGFEAILRSPDLPTAEAQIQIIEQLIAQKVASICIVGNDYDALTPV
                     LKKASAQGIKVFSLDSSVNPQARLTHVNQADSEKIGQTLIKAAYDMAGGKGEIAILSA
                     TSQASNQNIWIDFMKKELAKPEYKDLKLVKIAYGDDLRDKSVSETEGLLRSYPNLKVI
                     IAPTTVGIAAAGKVLTDKGLKGKVFLTGLGLPSEMAEYINNGVCPYMFLWNPIDVGYL
                     GSYVATGLVSGKITGKVGEKFSAGRLGNYTITQAPDGGTEVLLGPPFKFDKSNIEEWK
                     KVY"
     gene            complement(1149..2204)
                     /locus_tag="PLS88_07505"
     CDS             complement(1149..2204)
                     /locus_tag="PLS88_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU07227.1"
                     /translation="MGKSGLDINRPHALQRYQWEVFLFVLFLAVNVVNSFLSPYYLSL
                     DTFLSTPMNFLDKAFLVLPMTMIIILGNIDVSVGSIVALSSVLMAVSYNAGVPMPLAM
                     VLALLVSTTCGFINGFLQIKFRELSATIITLSTMTVYRGIAYVILEDRSAGKFPSWFS
                     FLAWGYIGKVPFIFIVFIIAAIIFSIILHKTRFGRMVYAVGNNRTASEYSGIRTDRVI
                     LGVSTLAGLMAGFTSLFLTSRMGSTRPNVALNYELDAIAMTVLGGISTSGGKGRIGGP
                     LIAIFLIGFLNYGLGLRNVSAQVILIILGILLILSVLVQNFTDRANRSPRKSATLAHR
                     SGVGDGSDIHKNSAQGG"
     gene            complement(2215..3285)
                     /locus_tag="PLS88_07510"
     CDS             complement(2215..3285)
                     /locus_tag="PLS88_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU07228.1"
                     /translation="MQSHKKIASSDIDQSLKDNAPTGSSKLSVSKLLQNSESFRELGL
                     LLFIIILSTIFQLRNPSFLSLANIKDLLANTAILSILSVGMMMVILTRGIDLSIGSTM
                     ALSGMVTALTVSANPEISPLLSLLEGMSIGLAAGSIIGVLIAYFNILPIIATLGLMNI
                     LRGMTYLVSKGKWVSAYQMSTGFKNLSTGTTLGINNLIIFAVIIYIFYSYFINQTKTG
                     RYIYAVGSSPETAELIGIKRRRIILLVYSLMGLLAGLAGVLWVSKFASAQGDTAVGYE
                     MNVIAATVLGGVSVSGGRGRVTGIILGSILFGILANALPLINISPFWQQFIQGIVILA
                     AIISNVLLQRRNERAALKKRAI"
     gene            complement(3272..4789)
                     /locus_tag="PLS88_07515"
     CDS             complement(3272..4789)
                     /locus_tag="PLS88_07515"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOU07229.1"
                     /translation="MPDYILELRHISKSFPGVQALKDVHFQLKPGEIHALMGENGAGK
                     STLIKIITGVYQPDQGEIYFNGQRIHFRSPTDAQRLGIAAVYQHVACFPDLSVLENIF
                     LGHESFDPVTRKINWRELRKKADDLLESLDADFSPSSTMGNLSIAQQQIVEIAKALSV
                     NAKILIMDEPTAPLSRRECEDLYRIVENLRDTGVSIIFISHRIEDMYRIAERVTVLRD
                     GAYVDTWKVPGLEQAQLVRGMVGREITQYFPSRHIQIGEEIFRVENLSRTGFFKDISF
                     SVRRGEILALTGLVGAGRTEICESIYGIAPRDSGRFFLEGKEISIRSPEEALSHGIGY
                     LPEDRMKQGLVLDWDLVRNITLASLKSFCQRGFMKQDQEREIAARLINDLKVKAAHIY
                     VKASTLSGGNQQKLIVAKLLTSKLKLIILDEPTKGVDVGAKTEIYRIMNQLAEQGYGI
                     IMISSEMPEVLGMSDRIVVLREGRKSAEFETAVATQEAILEAAMTSQRRQAHAIA"
     gene            complement(4803..6239)
                     /locus_tag="PLS88_07520"
     CDS             complement(4803..6239)
                     /locus_tag="PLS88_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOU07230.1"
                     /translation="MIENCAPNPTVGLFGIGLDTYWPQFPGLKERLAGYLTQTRTLLE
                     KASATVIDAGLVDTVQKARDAARLFREKRVSVVFLHITTYALSQTVLPIAQGIGVPII
                     IINMQPAKNIDYSWFNSLGDRALMTAEWLANCQSCCVPEIANVFNRNSIPYYLVTGFR
                     GEEFIEHELTSWVKAAQVAETLRSANIGILGHYYEGMLDVYTDVTLMAGTFGSQFNLI
                     EMDDLARLRKNVTASQIKEKIAQLRQEFDVVAECDLSELERAALTSVALDALVKEKNL
                     GALTYYYEGSPGSEHRDIVTSVIAGNTLLTAHHVPVAGECEVKNVIAMKILDLLGAGG
                     SFSEFYLIDYEDDVVLWGHDGPAHTGIAEGKVKLVPLPIYHGKPGKGLSIQMSVRHGP
                     VTLLSVVEGARGKVFLLTAEGESVSGPTLQIGNTNSRYRFPIPAREFSEAWSKQGPAH
                     HCAIGIGHVADEIEKVAALFGIECNRVC"
     gene            6388..7278
                     /locus_tag="PLS88_07525"
     CDS             6388..7278
                     /locus_tag="PLS88_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOU07231.1"
                     /translation="MEKTTEHTYFRYLTYSDEDEQWEMVCTDAGYAEVPPYTIYPPNK
                     DGHPRMFQKVAVGRTLNEYQIIYVTKGEGIFETMGKRYEVKPGSIIMVFPGIRHFYKP
                     VYEIGWMEYWVGFKGEHFEMLRERDFLNPGQAFFEIGLQNNILNLYNEIIDEVRNQKP
                     LYQIVATAKILSLISEIKACSRRQTQSSHTAKIVESAKCIMAEKIYGDIDIPSIASQL
                     GTSVSRFNDIFKTYTSMTPYQYYIHIKIHAAKSLLEQGDLSVKEVAYRLGFEDQYHFS
                     RLFKKKTGIAPSQWRAFMYE"
BASE COUNT         1975 a         1720 c         1647 g         1941 t
ORIGIN      
        1 tattccgctt catttccagg tttgagctgc atgacaaatg catagcgttt cattttttta
       61 tccttaaatc aatagggcac aatggaaatg gatattgcgg gcttgcatat ctcagcaagc
      121 ccgcataaat cgctcagtac acttttttcc actcttcgat gttgctcttg tcgaatttga
      181 atggaggccc gagcaatact tcggtaccgc catctggtgc ttgagtgatg gtgtagtttc
      241 ctagacgtcc agcactaaac ttctctccga ccttgccggt aatcttgcca ctcacaaggc
      301 cagtcgctac ataagatcca aggtaaccaa cgtcgattgg attccagagg aacatataag
      361 gacagactcc attattgatg tattcggcca tttcggaagg caatccaaga ccggtcagga
      421 agactttgcc cttgagcccc ttgtcggtga gcacttttcc agctgcggct atgccaactg
      481 tggttggggc tatgatcacc ttcaagtttg gataggatcg aagaagtcct tcggtttcgg
      541 agacggattt atcgcgcaga tcatcgccat aggcaatctt gacaagcttt agatctttat
      601 attctggttt ggcgagctct tttttcatga agtcgatcca gatgttctga ttcgatgctt
      661 ggctcgtcgc cgagagaata gctatctcgc ccttgccacc agccatgtca tacgcagctt
      721 tgatgagcgt ctggccgatc ttctctgaat ccgcttgatt gacatgggtt aatcgcgcct
      781 gtgggttgac cgaagaatcg agagagaaca ccttgatgcc ttgtgcgctt gctttcttta
      841 ggacaggcgt cagggcgtca taatcattgc cgacaatgca gatcgaggcg actttctgag
      901 caataagctg ctcgattatt tgaatttgtg cctcggcggt aggcagatcg ggtgagcgca
      961 gaattgcctc gaagccctgt tcctctatgc cgactctgaa tccctctagc tgtttttcgc
     1021 cgtatggatt gccagtgttc ttgaatacaa tggcaaattt tggcttctct gccgcaaata
     1081 cggcagatgg aatgataagc gccagtacga atagtataag cgctactcta gatactgtct
     1141 ttttcatatc agcctccttg tgctgaattt ttatgaatat ctgaaccatc tcccacacct
     1201 gaccggtgcg ctagagtggc cgatttcctt ggagatcgat tagctcgatc ggtaaagttt
     1261 tgcacaagca cagaaagaat gagaagaatc cctaagatta tgaggatgac ctgcgctgat
     1321 acattccgca gaccaagtcc atagttcaag aatcctatca agaaaatagc aataagcggt
     1381 ccaccaatgc gccccttgcc acctgaggta cttattccac ccagtaccgt catggcaata
     1441 gcatcgagtt catagttgag ggctacattc ggtcttgtac tgcccatgcg cgaagtaaga
     1501 aagagcgaag taaatccagc cataagccct gctagcgtgc ttacgccaag aattacgcga
     1561 tcggttcgaa tgccggaata ttcgctggcg gtcctgttgt ttccgactgc gtacaccatg
     1621 cgtccgaagc gagttttatg gaggataatc gaaaaaataa tcgcggcgat gataaacact
     1681 atgaagataa agggtacttt acctatgtat ccccaagcca aaaagctgaa ccatgaaggg
     1741 aacttcccag ccgagcgatc ttcgagaatc acataggcaa ttccacggta aaccgtcata
     1801 gtgctgagtg taataatcgt tgcggaaagc tctctaaact tgatctgaag aaaaccattg
     1861 ataaaaccgc aggttgtgct tacaaggagg gctaaaacca tagccagagg cataggaacc
     1921 cctgcattat aagaaactgc catcaacaca gagctgagcg ctacgattga acccaccgat
     1981 acatcgatgt tgccaagaat aataatcatc gtcatgggca aaacgagaaa ggccttatcg
     2041 agaaaattca tcggcgtgct caaaaatgta tcgaggctaa gataatacgg gctaagaaat
     2101 gaatttacga catttaccgc cagaaaaaga acaaagagaa aaacttccca ctggtatcgt
     2161 tggagcgcat gcggtctatt gatatcgagc cctgattttc ccatctcttt cctctcaaat
     2221 tgcgcgcttt ttaagcgctg ccctttcgtt tcgccgctgc aacaaaacat tagagattat
     2281 ggcagcaagg atgacgattc cttgaatgaa ttgctgccaa aatggtgaaa tattgattag
     2341 tggcagggca tttgcaagaa ttccaaagag aatcgatccc aaaataatgc ccgttactct
     2401 tcctcgcccg ccggatacac ttacaccgcc caaaaccgtc gcggcaatta cgttcatttc
     2461 atatccaact gccgtatctc cctgtgccga tgcaaatttg cttacccaca gcacaccagc
     2521 caatccagca agcagaccca taagcgaata gacaaggaga ataatgcgtc ttcgttttat
     2581 accaataagt tcggctgtct ctggacttga acccacggca tagatatatc gacctgtctt
     2641 ggtttgattg ataaaatagg aataaaaaat ataaattatt actgcaaaaa taattagatt
     2701 attgatgccc aaggtagtac ctgtgctcag atttttgaaa cccgtcgaca tttgataagc
     2761 gctgacccat ttacccttgc taacaaggta ggtcatgcct cgaagaatat tcataaggcc
     2821 gagagttgca ataattggaa gaatattgaa gtaagcgata agcacaccga tgatcgaacc
     2881 ggcagcaagt ccaatcgaca ttccctctag caaagatagc aaaggcgata tctctggatt
     2941 tgcgctcaca gttagcgctg tcaccatgcc agatagagcc attgtggaac caatcgacag
     3001 atcgatgcct cgggtcagaa tgaccatcat catacctacc gaaagaatgc tcaaaattgc
     3061 ggtattggca agtaaatcct tgatattcgc caggcttaaa aaactgggat ttcgcagttg
     3121 aaatattgta gataggatta ttataaagag aagcaaacct agctctcgaa atgattcgga
     3181 attctgcaac agtttagata cactcaattt ggatgagcca gtcggagcat tatctttgag
     3241 agactgatcg atgtcgcttg atgctatctt tttatgcgat tgcatgcgct tgtctcctct
     3301 ggctagtcat tgccgcttct aggatcgctt cctgcgtggc tactgctgtc tcgaattctg
     3361 cggattttcg accttcgcgg agcaccacta tgcgatcgct catgcctaga acttcgggca
     3421 tctcgctcga aatcattata atgccgtagc cttgttccgc gagctgattc atgatgcggt
     3481 aaatctcggt ttttgcgcct acatcgacgc ctttggtcgg ttcgtccaaa ataatcagct
     3541 tgagcttgga agttagaagc ttagccacga tgagtttctg ctgatttcct ccagagagtg
     3601 tggaagcttt tacatagatg tgagcggctt ttaccttgag atcatttatc aagcgtgccg
     3661 caatctctcg ctcttggtcc tgcttcatga agcctctttg gcagaaggat ttgagagatg
     3721 caagggtgat attgcgaacc aaatcccagt cgagcacaag gccttgtttc atccggtctt
     3781 caggtaggta tcctatacca tgagatagag cttcttccgg agaccgaatc gagatttcct
     3841 ttccttcgag aaagaatctg cccgaatcgc gaggtgctat accgtatatg ctttcgcaaa
     3901 tttcggttcg tccagcaccc acaaggccag tcaacgctaa gatttctcca cgccgaaccg
     3961 aaaaggaaat atccttgaaa aaccccgtgc gggaaaggtt ttccacgcgg aatatttctt
     4021 ctccaatttg aatatgtctt gaagggaagt attgagttat ctcacgaccc accattcctc
     4081 gtactaactg cgcttgttcg aggccaggta ccttccaagt atcaacatag gcaccatccc
     4141 ttagaactgt aacacgctcg gctatccgat acatatcttc gattcgatgc gaaatgaaga
     4201 ttatcgatac gccagtgtcg cgcagatttt ccacaattcg atagaggtct tcgcattcgc
     4261 gtcgagacaa aggggcggtt ggttcgtcca tgataaggat cttggcgttt acggatagag
     4321 cctttgcaat ttcgacgatt tgttgctgcg caatcgaaag gttgcccatg gtggatgagg
     4381 ggctaaaatc cgcatctagg ctttcgagga gatcgtcggc cttcttgcgc agctcccgcc
     4441 aattgatttt gcgagttact ggatcgaagc tctcgtggcc caagaatata ttttcaagca
     4501 cggagagatc tggaaagcag gctacatgct ggtaaactgc cgcaattcca agtctctgtg
     4561 catcagtagg gcttcgaaaa tggattcgtt gtccattgaa atagatctcc ccctggtctg
     4621 gttggtatac accggttatt atcttgatga gagtcgactt tccagctccg ttttctccca
     4681 taagagcatg aatttctcca ggctttagct gaaaatgcac atctttgagt gcttgaacgc
     4741 cagggaaaga cttggatata tgtcttagtt ccaaaatata atcaggcatt ataccttcct
     4801 tttcaacaga cgcggttgca ctcgatgcca aatagagcag ccactttttc gatttcatcg
     4861 gcaacatgcc ctatgccaat agcgcaatga tgcgctggtc cctgtttaga ccaagcttcg
     4921 gaaaactcgc gtgctggaat agggaatcta tagcggctat tggtgttgcc aatctgcaaa
     4981 gtcggtccag ataccgactc gccttctgca gtcaggagaa atacttttcc tctggcgcct
     5041 tcgactacgg acaagagcgt aacggggcca tgtcgaacgc tcatctgaat ggagaggcct
     5101 ttaccaggtt ttccatgata gatagggagc ggtacgagct taactttacc ttctgcaatg
     5161 ccagtatgcg cgggaccatc atgcccccat aaaaccacat catcctcgta atcgatcaga
     5221 tagaactccg agaacgagcc tcccgcgccc aataggtcta ggattttcat ggcaattaca
     5281 ttctttactt cgcattcgcc agcaaccggc acatgatgcg cggtcaataa tgtattccca
     5341 gctataaccg aggttacaat atcccgatgc tcgctgcctg gagagccttc gtaataatag
     5401 gtcagagctc ccagattctt ttcttttacc agagcgtcta gggcaacgga tgttaaggca
     5461 gctcgctcta actctgacaa gtcacattca gccaccacat cgaattcttg tcgtagttga
     5521 gctattttct ctttaatctg agatgcagtg acattcttgc ggagccgcgc cagatcatcc
     5581 atttcgataa gattgaattg agagccgaag gtgcccgcca taagcgtaac atcggtataa
     5641 acatcgagca tgccttcgta atagtgacca agaattccta tgttggcaga tcgcagagtc
     5701 tcggcaactt gggcagcctt gacccagcta gtcagttcat gctctatgaa ttcttcaccg
     5761 cggaatccgg tgaccaaata gtaagggatg ctatttctgt tgaatacatt ggcaatttcg
     5821 ggcacgcagc aggattggca gttcgcaagc cattcggcag tcattagcgc tcgatcgcct
     5881 aggctgttga accaagaata atcgatattc tttgcgggtt gcatgttgat aattattatg
     5941 ggtactccaa ttccttgtgc gataggcaac accgtttgcg agagcgcata ggttgtgata
     6001 tgcaagaata caaccgagac tctcttttct cgaaagagcc ttgcagcgtc tcgggctttt
     6061 tgcaccgtat ccactagtcc cgcatctatg actgttgcgc tcgctttttc taataaggtg
     6121 cgagtttgcg ttagatatcc agcaagtctt tctttgagtc cggggaattg aggccaatag
     6181 gtatctagac cgattccaaa gagcccaaca gtgggatttg gtgcacaatt ctcaatcata
     6241 tttcaattag tataggtcag ctttggtagg acacaatgga tattattgtt tttatgatag
     6301 ataattcttg cataagggaa agaaaatgag catcttgcct gttgcaatag atcccactat
     6361 aagggcaata ttaagagaga aaagcccatg gagaagacca cagaacatac ctattttcgc
     6421 taccttacct actcagacga agacgaacaa tgggagatgg tctgcacaga cgcaggttat
     6481 gccgaagtgc cgccttatac catctatcct cccaacaagg atggacaccc tcggatgttc
     6541 cagaaggttg cggtgggcag aacactcaac gaatatcaga tcatctatgt cacaaagggc
     6601 gagggcatct tcgagaccat gggtaagcgc tacgaagtaa aaccaggctc tataatcatg
     6661 gtctttccag gcattagaca tttctacaag ccggtctatg agattggatg gatggagtat
     6721 tgggtgggct tcaaaggaga gcatttcgaa atgctccgag agcgagattt tctaaatcct
     6781 gggcaagctt ttttcgagat aggcttacag aacaacatcc tcaatctata taatgaaata
     6841 atcgacgaag ttcgcaacca aaagccactt tatcaaatag tcgctacagc caagattctc
     6901 tcactaatct cggagataaa agcttgctcg cgcagacaga ctcagtccag ccatacggcc
     6961 aagatagtcg aatccgcaaa atgcatcatg gccgaaaaaa tatatgggga tatagacatt
     7021 ccaagcattg caagtcagct tggcaccagt gtctcccgct tcaacgacat cttcaagact
     7081 tatacttcta tgacacccta tcaatattat atccacataa agatacacgc ggcgaagagt
     7141 ctactcgaac aaggcgatct ttcggtaaaa gaagttgcct atcgtctagg ctttgaagac
     7201 cagtatcatt tttctaggct tttcaagaaa aagacaggga ttgccccttc gcaatggcga
     7261 gcctttatgt atgaatgaga ttg
//
LOCUS       DAOSOB010000075         7271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_10277_length_7271_cov_64.541574, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000075 DAOSOB010000000
VERSION     DAOSOB010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10277_length_7271_cov_64.541574"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1112
                     /locus_tag="PLS88_07530"
     CDS             99..1112
                     /locus_tag="PLS88_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07232.1"
                     /translation="MAEKKICRSADISNLYQDNDDYPLGPESHPRGRGEAAIIYPVIS
                     RRAGGLSLGINLFPERKVCSYNCPYCEVLPFSNPGASLKSGDVSRAIQRFFDEEWSEL
                     SKTLELKDISISGNGEPTLSPFLEEALYAARQAIETIANAKDCAVRFPQHSIPTLVLI
                     TNSTGFLHESIRELLIGFYKNFPFEIWAKLDGGTEKLHTQLSRSSFPFAEIVRSITDF
                     SRSVPLTLQTMVIYDNRTGNVYFDCQAYAATINKILNNGGQIKAIQLYTVARVPVESW
                     VVPVSDKEIVSFARQLSSLLDKLPKEKAINEKKEALQNEAYSLPIFCYGSQGKLDWDH
                     ADR"
     gene            1099..1950
                     /locus_tag="PLS88_07535"
     CDS             1099..1950
                     /locus_tag="PLS88_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU07233.1"
                     /translation="MPTADLHTHSNASDGELTPTALVEKARALGLSTIALTDHDTTQG
                     LAEARAAAALEKLEFISGIEIEIDFEPGEFHLLGYGIDEAAPSLVEAVERLARARHER
                     NLAIIERIMDAKLPLDFARIESISRTSYLGRPHLADLLVEAHCAHNRQDAFDRYLGKG
                     KPFYIKKACLALEEALSIIDTAGGVSVIAHPYSLFVSKAVLTTLFSQWKEMGIRGIEA
                     YHPTAKYGQCVILDRMARDQGMFVTAGSDFHGKMRPECGLGRTSGNMPIDMRFRDQLV
                     AALESRT"
     gene            complement(2062..3168)
                     /locus_tag="PLS88_07540"
     CDS             complement(2062..3168)
                     /locus_tag="PLS88_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOU07234.1"
                     /translation="MKTANPRKISFLILFLVLLLLVGRLFYPFLSVILWSGLLYVFLE
                     PLYKKLKGPEGIKGRKTMRARLSALFLSILGVIIILIPLAFLAFETIQQLADLLKSSI
                     RFVEDNIDKLRIDSQGQIGSAIQSLLGDLYDVSTFDPMRELQSFLASGVNQLLKISTS
                     LIKNISKFVITICFMIFTLYYLLMDASSLGDTFVSMMPIAPQYTRLFMSKLRETGRQL
                     VIGYFLVSLFQGFMMFLLSIIFGFKNSLVLAVLTAISSFVPLLGTTVVWAPMGIYLAV
                     NGQIIKGIIFVACASVVVATLDNFIRPIVLGEQIKIHPLLLFFSITGGLAVFGFNGLI
                     LGPIILILFIAAGDLYRALNEESEFSNKKSESES"
     gene            3304..4011
                     /gene="rsmI"
                     /locus_tag="PLS88_07545"
     CDS             3304..4011
                     /gene="rsmI"
                     /locus_tag="PLS88_07545"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOU07235.1"
                     /translation="MSTLFMIATPIGNLGDISLRALETLNSVDIIACEDTRHTLKLLS
                     HYDIHKPLISCHSYNEQQGAKKIIGLLSEGKEVAYCSDAGTPGLSDPGALLVQMARAA
                     GYKVVPIPGPSAFACLISASGLQGRTFLFDGFLSPKAGKRRTRLRELLDRGEPFILYE
                     SPFRIGKLLADIASIDPERRLCIGRELTKIHEEITEGKAKELALRFFAEPAKGEFCIL
                     VHGEKPESYADCMENES"
     gene            4008..4823
                     /locus_tag="PLS88_07550"
     CDS             4008..4823
                     /locus_tag="PLS88_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOU07236.1"
                     /translation="MMDISHFENLHGRARLRKAVLLLERLEREWRKTDPLFISLEDIV
                     FVRQIAQILADSKETPLECVKAASEFLSILSGLSDGLDHSGFLQAENRFRHRLIVISG
                     QSVADWDFIAVPRPSSSHTIKRAGMRVFLEDLRSPFNIGSIFRTAEALGFEEIILSPE
                     CADPMHPRAIRSSMGTIERMQWRRAELSELDCVKGVFALEVGGIPLNKFSFPFPGLMV
                     LGSEELGVSNKALQYCSAGCVEIPLVGTKASLNVATAFGIAGYSWYYSRNLQA"
     gene            4897..5787
                     /locus_tag="PLS88_07555"
     CDS             4897..5787
                     /locus_tag="PLS88_07555"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU07237.1"
                     /translation="MSFNPFMKKLGLSVLDRAVIVHVDDVGMCLSSIEAFFELYDFGT
                     VTSGSLMAPCPWFPEVARRCAARPDVDVGIHATLTSEWKGYRWGPVSTRDPDSGLLDA
                     EGYFPRTSEELQSRAKPEAAMREIAAQVAMVYAAGIEPTHIDTHMFSVAHERLIDAYI
                     SQAIEHRIPAMLPKPDIAGFESIVAGYETRDCLAGYLRELGEKGMPLLDAVVAMPLDE
                     PCDQTETVKRLFGSLKEGITHFVLHASTDSPELRAITPDWESRVWNYRTMHSDTMKRI
                     MESEGIVLIGYRDLRDALRK"
     gene            5927..7048
                     /gene="ugpC"
                     /locus_tag="PLS88_07560"
     CDS             5927..7048
                     /gene="ugpC"
                     /locus_tag="PLS88_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOU07238.1"
                     /translation="MAKVELKGIGKVYEGNVRAVSDANITINDKEFVVFVGPSGCGKT
                     TTLRMIAGLEDITEGEIYIDSKLVNDVPPKDRDIAMVFQNYALYPHMTVYDNMAFGLK
                     IRKLPKEEIETRVKEAARILDIEKLLDRKPKQLSGGQRQRVAVGRAIVRKPKVFLFDE
                     PLSNLDAKLRVQMRAELIELHDRLQSTMIYVTHDQVEAMTMGDKIVVMKDGIIQQIGS
                     PLYLYNNPINKFVAGFIGSPPMNFMSVDVIEEGGQVMVDEGSFKLAPDEKHAALLKPY
                     IGKSVYFGIRPEDLPVADAAHRGQKFNAKVTVVEPLGSEIHLQATTPTQGMVSRIPPH
                     HIYKHGDTIMFAPVMAKAIYFDKETEKSILPVKWNEQEA"
BASE COUNT         2010 a         1626 c         1697 g         1938 t
ORIGIN      
        1 gtaactaaaa gttagctaat attatatata aaattttatc atatagaatc attggctctc
       61 gccaaactcc gatcgaaaca ttattataat tttatatcat ggcagaaaag aagatttgtc
      121 gcagtgcgga tattagcaat ctttaccaag ataacgatga ctatccgctt ggtcctgaat
      181 cccatccgag gggtagagga gaggctgcaa tcatctatcc ggttatttca cgacgagctg
      241 gagggctttc attaggcatc aatcttttcc ctgagcgcaa ggtctgcagc tataattgcc
      301 cctattgtga ggttttgccc ttttcaaatc ctggggcttc actaaagtct ggcgatgtga
      361 gccgcgctat ccagcgcttt ttcgatgaag aatggtcaga gctctccaag actttggagc
      421 tcaaagatat ttcgatatct ggaaatggcg aacccacgct ctctcccttt ttggaagaag
      481 ctctgtatgc tgctcgccaa gcgattgaga ccatagctaa tgcaaaggat tgcgcagtgc
      541 gctttccgca gcattcgatt ccaactctcg tgcttatcac taattccacc ggttttctac
      601 atgaaagtat tagggagctt ctaattggtt tttacaaaaa ctttcctttt gaaatctggg
      661 caaagctcga tggcggtact gaaaagcttc atacacagct ttcacgctcg agttttccgt
      721 ttgcggaaat tgtgcgcagc attacggact tttcgcgcag tgtccctctt actctgcaga
      781 ctatggtgat atatgacaat cgaacaggca atgtttactt tgattgccaa gcctatgctg
      841 ctactattaa taaaattcta aacaatggcg gacaaataaa ggcaattcag ctctataccg
      901 tggcaagggt tccagtcgaa tcatgggttg tgcctgtatc cgataaggaa atcgtgagct
      961 ttgctcggca attatcttcg ctattagaca agctcccaaa agaaaaagct atcaacgaaa
     1021 aaaaggaagc cttacaaaac gaggcctatt cgcttccaat cttctgttat ggatcacagg
     1081 ggaaactgga ctgggatcat gccgaccgct gaccttcaca ctcactccaa tgcctccgat
     1141 ggcgagctta cgcctaccgc actcgtcgaa aaagcccgcg cgctcggcct atccaccata
     1201 gcccttaccg accacgacac tactcaaggc ctagcggaag cgcgcgcggc tgccgccctt
     1261 gagaagctcg agttcatatc tggcatagag atcgagatag atttcgagcc cggtgagttt
     1321 catcttcttg gttatggtat cgacgaggct gctccatcgc tagtagaagc ggtggaaaga
     1381 ctcgctcgcg cccgacatga gcgcaactta gcaatcatcg agcgcattat ggacgcaaaa
     1441 ctccctcttg actttgcacg aatcgaatcc atttccagaa cctcctatct aggtaggccg
     1501 cacctcgcag atctccttgt cgaggcgcat tgcgctcaca accgtcagga tgcttttgac
     1561 cgctatcttg gcaaaggaaa gccattctac attaaaaaag cctgtctagc gctcgaagaa
     1621 gctctctcga ttattgatac tgctggtgga gtctcggtta ttgctcatcc ctattcactt
     1681 ttcgtctcaa aagccgttct tacaacgctt ttttctcaat ggaaagagat gggcattaga
     1741 ggaatagaag cttaccatcc aacagccaaa tatggtcaat gcgttattct ggaccgaatg
     1801 gcccgcgacc aaggaatgtt tgttactgct ggctcagatt ttcatggcaa gatgaggcct
     1861 gaatgtgggc tggggcgcac ttctgggaat atgcctatag atatgcggtt tcgtgatcag
     1921 cttgtcgctg cccttgagag tcgtacttga tattcatatc tgaaaatcgc tactaattgc
     1981 cgctaaatct caaagagact ttaagaacaa tagcttttaa tgacaacttc atttgacaac
     2041 aagagccctt agaattagcc actatgattc gctctcgctt tttttgttag agaactcgct
     2101 ctcctcgttc aaagctctat acaaatcacc agccgcaata aaaagtatga ggataatagg
     2161 gccaaggatc agcccgttga atccaaagac agccaagcca ccggttatgg agaaaaaaag
     2221 caaaagggga tgtattttta tctgttcacc cagcacaatt ggcctaataa aattatcgag
     2281 tgtcgccaca accacgctgg cacaggcgac aaaaataatt cccttaataa tctgaccatt
     2341 caccgcaaga taaatcccca ttggagccca tactacagtg gtaccaagca gtgggacaaa
     2401 agatgaaatg gccgtcaata ctgccagtac aagactattc ttgaaaccga agataatgct
     2461 caggaggaac atcataaaac cttgaaaaag cgaaaccaga aagtacccga tgacaagttg
     2521 acggcctgtt tcgcggagct tgctcataaa aagccgagta tactgaggag ctatgggcat
     2581 cattgaaaca aatgtgtctc ctaaggaact tgcatccatt aaaagataat acagagtgaa
     2641 tatcatgaaa caaatcgtga ttacaaattt ggatatattc tttatgagcg aagtggatat
     2701 ttttagcagt tggtttactc cggatgcaag aaacgactgc agctctctca taggatcaaa
     2761 agtgctcaca tcatatagat caccgagcaa agactgaatt gctgaaccta tttgaccctg
     2821 agaatcgatt ctcaacttat cgatattgtc ctctacaaag cgaatactcg atttgagcaa
     2881 atctgcaagc tgttgtatcg tctcgaatgc gagaaatgca agaggaataa gaatgatgat
     2941 aacgccaaga atcgacaaga acaaagcgct caatcgggcg cgcatggttt ttcggccttt
     3001 tatgccttca ggtcctttga gctttttata aagcggttcg agaaagacgt atagtaagcc
     3061 actccataat attacgctca gaaaaggata gaaaagcctt ccgaccagaa gcaataatac
     3121 gaggaatagg atcagaaatg atatctttcg tggatttgca gttttcattg tccgttatga
     3181 taattattat tgaggcttat ggatagatgc gaattattta gagttcggtg cgagctttgt
     3241 tgcactatag gtatttagga aaagcctaga cataatggct cgtaatcgta cactattgtc
     3301 tttgtgagca cactattcat gatcgcgaca cccattggca atctcggaga cataagtcta
     3361 agggcgctcg aaacactcaa ttctgtcgat attattgcct gtgaggacac tcggcacaca
     3421 ttaaagctgc tttcgcacta tgatattcat aagccattaa tctcatgcca ttcatataat
     3481 gaacagcaag gagcaaaaaa aattattggc cttttaagcg agggtaaaga ggtggcctat
     3541 tgttcagatg caggaactcc tgggctctca gatccaggag cgttactggt gcaaatggcg
     3601 cgggcagcag gatacaaggt cgtacctatt cccgggccaa gcgcttttgc ttgtcttatt
     3661 agcgcatctg gactacaggg aaggacattt ctgttcgatg gttttctttc accaaaagca
     3721 ggcaagaggc ggacaagact aagagagctt cttgaccgtg gagaaccctt tattctctat
     3781 gaatcgcctt ttcgaatcgg aaagctgctt gctgatatag cttcaataga tccagaaaga
     3841 aggctgtgta taggcagaga gctcacaaaa atacatgagg agataacaga agggaaggcc
     3901 aaagagctag ctcttcgatt ttttgcagaa ccagccaaag gggagttttg tatacttgtg
     3961 catggcgaaa agcctgagtc ttacgcggat tgtatggaaa atgaatcatg atggatatat
     4021 ctcactttga gaatcttcat ggacgtgcaa gattgcgaaa agccgtgcta ttgctcgaac
     4081 ggctagaaag agagtggcgg aagaccgatc ctctttttat ttctctcgaa gacattgttt
     4141 ttgtgaggca aatagctcaa attcttgccg attcaaaaga gacaccgttg gaatgcgtca
     4201 aggctgccag cgaatttctc tcgattttgt ccggtcttag cgatggtctt gatcacagcg
     4261 ggtttttaca agctgagaat agatttcggc atcgactcat agtcatatct ggccagagtg
     4321 tagcggattg ggatttcata gctgtaccca gaccttcgag tagccatacc ataaaaagag
     4381 ccggcatgcg agtattttta gaagacctac gctcgccttt caatatagga agcatctttc
     4441 gcaccgccga agctcttggt ttcgaagaga taattctttc gccggaatgt gcggatccta
     4501 tgcatccaag ggcaatccga agttcaatgg gaaccataga gcgcatgcaa tggcgcagag
     4561 ctgaactctc tgagctcgat tgcgttaagg gagtttttgc tcttgaagtt ggtggtattc
     4621 ctctcaataa gttctctttt ccatttccag ggttaatggt ccttggctct gaagagcttg
     4681 gagtctccaa taaagctcta caatactgca gtgcaggatg cgttgaaatt cctctagtag
     4741 gaacgaaggc ttcgcttaat gtggcgacag cctttggtat tgcggggtat tcatggtact
     4801 attctagaaa tctgcaagca tgatttgctt atattttgcc atgctgtttt gccgctagca
     4861 ctagcactga ggcgatgcag tcgaaaggaa catagaatgt cttttaatcc ttttatgaag
     4921 aagcttggtc tttcggtttt ggacagggcg gtcatagtcc atgtggacga tgtaggaatg
     4981 tgcctttcat cgatagaagc attttttgag ctatacgatt ttggcactgt gacatccgga
     5041 tcgctgatgg caccttgccc ttggttcccc gaggtggcgc gccgctgtgc ggcgcgccct
     5101 gatgtagatg ttggcattca tgcgactctg acaagcgagt ggaaaggcta ccgctgggga
     5161 ccagtatcga ctcgcgatcc agattccggt cttctcgatg ccgaaggata ctttccgagg
     5221 acaagcgagg agcttcagtc tagagctaaa cccgaagctg caatgagaga aatagcagct
     5281 caagtagcca tggtatatgc tgcaggtatc gaacctaccc atatagacac ccatatgttt
     5341 tcggtggcgc atgaaaggct aattgatgcc tatatatccc aagccattga acataggata
     5401 cctgccatgc ttccaaagcc agatatcgct ggtttcgagt ccattgttgc aggttatgaa
     5461 acccgcgact gccttgctgg atatcttaga gaactagggg aaaaaggcat gcccctattg
     5521 gatgctgttg tagctatgcc cctagacgag ccttgcgatc agaccgagac tgtcaagcgc
     5581 ctttttggtt cccttaaaga gggtatcacg cattttgtgc ttcacgcttc aaccgacagt
     5641 cctgagctaa gggccataac ccccgattgg gagagcaggg tctggaatta cagaaccatg
     5701 catagcgata ctatgaaaag aattatggag agcgagggaa tcgttctaat tggctaccgt
     5761 gacctccgcg atgcattgag gaaatgatct ggacatctcc gaagctgggt gaaggattta
     5821 tgcatagaaa atcaagctgg ggtatacgat aagcctcggt tgacgccttt tataaatcat
     5881 cgtaaactgt cttttatata acatacacct cttgaaagga ggatccatgg caaaggttga
     5941 actaaaaggt attggaaagg tgtacgaagg caatgtgaga gcagtctcgg atgccaacat
     6001 caccatcaat gacaaggagt tcgtggtctt cgtaggtccg tcgggctgtg ggaagacaac
     6061 gacgctccga atgatcgcag gactcgaaga tatcactgaa ggcgaaatat acatcgatag
     6121 taagctcgtc aatgatgttc cacccaagga tagagacata gcgatggtgt tccaaaacta
     6181 cgcgctttat ccccacatga ctgtctatga caacatggct ttcggtctga agattcgcaa
     6241 attaccgaag gaagagatcg aaactcgtgt aaaggaagcg gcgcgaatcc tagatatcga
     6301 aaagttgctc gaccgcaagc ccaagcagct ttcgggtggg cagcgtcaac gagtagcggt
     6361 gggtcgcgcc attgtgcgta agcccaaggt ctttcttttc gatgaacctc tatcgaacct
     6421 cgacgcaaaa ttgcgtgtcc agatgcgcgc cgagcttatt gagttacacg accgtctaca
     6481 gtctacaatg atctacgtaa ctcatgacca agtagaggct atgacaatgg gcgacaagat
     6541 tgtcgtcatg aaggatggaa ttatccagca gattggctct cccttgtacc tctacaataa
     6601 tccaatcaac aagtttgttg ctggcttcat aggctctcct cctatgaact tcatgagtgt
     6661 cgatgtcatt gaagagggtg gtcaggttat ggtcgatgaa ggttccttca aactcgctcc
     6721 tgatgagaag catgctgcgc ttctcaagcc ttatatcggc aagagtgtct attttggtat
     6781 cagaccagaa gatctccctg tggctgacgc tgcacatcgt ggccaaaagt tcaatgcaaa
     6841 ggtcacggtg gtcgagccac taggttcgga aatccacctt caggccacca cgcctaccca
     6901 gggcatggtg tcgcgtattc cgcctcacca tatttataaa cacggcgata ccatcatgtt
     6961 tgctccagta atggcaaaag caatctactt cgataaggag acagagaaga gtattcttcc
     7021 cgtaaaatgg aacgaacagg aggcctgagg cttataccga aagccgaagc cctattatct
     7081 taaaaaatga tctgttgatt gctatttttt atagtaataa atcgaaaatt aaaagccgcc
     7141 tcaaatagag gcggcttttt tagggccaat tattcggtct ctttagattc tttttttgcg
     7201 cgtttctctg cgcgcttttc cttaagcgtt ttctggggtt ttttcttctc ggccgtctta
     7261 ggtttatcca t
//
LOCUS       DAOSOB010000076         7203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_10392_length_7203_cov_75.952434, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000076 DAOSOB010000000
VERSION     DAOSOB010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10392_length_7203_cov_75.952434"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..768)
                     /locus_tag="PLS88_07565"
     CDS             complement(31..768)
                     /locus_tag="PLS88_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07239.1"
                     /translation="MSKVYSKFDFAGKNKRPESFLIFCLYACFIFLLTTCSSDSLSFP
                     EGFVQFYPKLEAWENRSEGFTLVSISSADGAQGFFEWDAEGIYGRIVEVGASKDVRFF
                     SIGDGGMNGLALEAKKKGKSLEIFYNSMLQNSYVSQGESNQHKISVDRLKAIEDLRLL
                     GGMFEETQENGRKISMRLQYLVSRKDFEERAFDDFLRRGNSPYKMAEFRRARLEASSL
                     VKYDLPEDSGNSDSESTKNVSILFQLT"
     gene            1012..>2605
                     /locus_tag="PLS88_07570"
     CDS             1012..>2605
                     /locus_tag="PLS88_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07240.1"
                     /translation="MKFVKRFLPLLAIVALVLASCATQAGQVPKGAETPPSVAESEPQ
                     QPQVQPEAEVEKPSVEKSELQSLFEQANDLKDEAIKFGLDSILSDKFQEANKKHDTVS
                     AKYKDLVEAPAQYDGAKAYPLKDELAEVVSTWDELIQEGNLICPEMVKMERNGAELMR
                     SIAMDADAPMMASAQYDIGLQYCAQAEIYADDSRFLDAIDSYKLASAAFENSAIAAEV
                     NKLRENIENNGYSSYESIQMYLVMGEEKYKEAQDLWQKGSIEDIAASTSVLKEAKEYY
                     YEVNSKGSNFKALEAKDKAIAAQDEAIKVNADLNAPDEYQNALDLFAEAEKNMEEADY
                     ISAYSNYIDAATVFEAARDISQAMAFDAASAIAEAEARFAEAKDRASELGLEDNIYLI
                     EASLHLDNAKALERELKYSAAIFETNEVSNYIGLSDSFVQQEAEIREKARLDQLEKDK
                     AASEKAIADAKARIAWADQVDLKSDYPKQYESASNAMAAAEKAYQIEKYAAAKTLAEE
                     VSTTLSDDFQKQVLAARELAKIK"
     assembly_gap    2606..2705
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2857..3549
                     /locus_tag="PLS88_07575"
     CDS             2857..3549
                     /locus_tag="PLS88_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07241.1"
                     /translation="MVWADLNNIKADYSAVYNSAHSAMEAAEKAYQLEKYAAATKLAD
                     EVLSTLSPDFEAKVAADRAEKETQELVAQNKKYAETAISDAKSRYDWAASKNAANNYP
                     DLFKEGGDLLADAQTAFNALDYVRAKDLAAQAYWTLMEIGEFAPLPATYKVRLIPERR
                     DCLWRIAEYPFVYNNPYKWPVLYEANKKTFKDPSNPNLIFPDQVLTIPSIKGEVRKGA
                     WDPKKTYQPLSK"
     gene            complement(3622..4947)
                     /locus_tag="PLS88_07580"
     CDS             complement(3622..4947)
                     /locus_tag="PLS88_07580"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOU07242.1"
                     /translation="MEQMIKNLMDFVDASPTPFQAIDNLTGILEAHGAIKLDEREIWQ
                     LEPGVSYYVVRGSCSLVAFRMGLKPPSESGFLIAGAHTDSPALKIRPEKELQSRGYLR
                     LALESYGSPILSGWLDRPLGVAGALAVLENKDIHQRFYISHEPIAVIPNLAIHLNRDI
                     NKGFEYNLNQQLPALFGLATLNRKESESESESEKENETASASYSEIMMRISQELGVDP
                     KTIISSDLRLYDTEPSRFFDNSLINAPRLDDLAGCAALIEAFISVQAQASTQVACFFD
                     AEEIGSMTVGGAQSSYLRDVLARITLASRSLGQDLYRALACSACVSIDGAQAWHPGYP
                     DKFDDFYTPLLGRGIALKSNANMNYATDFTSRQIGHTIASKAGIKTQLYMARADIQPG
                     KTIGPITASRTGVPTVDVGHPLLAMHAIRETIAASDHLDMIAYLRAFYF"
     gene            complement(4910..5350)
                     /locus_tag="PLS88_07585"
     CDS             complement(4910..5350)
                     /locus_tag="PLS88_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU07243.1"
                     /translation="MIYLSIGQLERMLSIPASTLRFWEQEVPLLMPSRTKSGRRNYSL
                     SEVVLIARLKHLALDKKLGLGRAQRILEYELLYQDQELRANIKQLREELFRLLLDCDR
                     WKASLGELIAVSTAESSVKENSVEKNLGKELLDGTDDKELNGFC"
     gene            complement(5354..6799)
                     /gene="der"
                     /locus_tag="PLS88_07590"
     CDS             complement(5354..6799)
                     /gene="der"
                     /locus_tag="PLS88_07590"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOU07244.1"
                     /translation="MSKKKQTAEPLEIDLQQKGALSQSKEKEESSPNIRYENLPLVVL
                     AGRPNVGKSTLFNRLIGKRKAITDAKPGVTRDPIEEECELLGTNKRICLVDTGGFKLE
                     REGLDELVVARALAYIQRADLIIFIVDITQITPEDEEFAAFLRPYAKKLLLVVNKADS
                     PERDALVWEHARWGLGQMLFISAEHNRNIDKLAEAIVARLDFSNIQQVEVEKADIHLA
                     IMGKPNTGKSTLLNALVGYERSIVSPEPGTTRDIVKDRFQYRGRGIVVLDTAGIRRKK
                     KVTDNVEYYSVTRSIRIVSQADVVVLLIDAEEGLSDQDKKIAAFAVEAGRPVVFALSK
                     WDKMPDIKNAFEATRDRLRFFFGQMAYAPVVAISAKEGKGLDKLMSTVVSLYAKANKV
                     IETSRLNQAVAEWIQETPPPAGPSTRFKLRYAVQSSVNPQRFIFFVTRPEAVPESYKS
                     FLKNKIRFDFGLDGVPIQVELRASRRDRLRS"
     gene            complement(6808..>7203)
                     /locus_tag="PLS88_07595"
     CDS             complement(6808..>7203)
                     /locus_tag="PLS88_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOU07245.1"
                     /translation="VLSRSCDFFSIGTNDLSQYTLAVDRGNQKVAYLNNPFDIAVLRL
                     ISMTIKSSKKANIGVSLCGEMAADPASAVLLVGLGLRSLSMSASAIPAVKRALMSITM
                     EEASSLAQQALSMNTASQVTALLNSRLNL"
BASE COUNT         2041 a         1652 c         1528 g         1882 t
ORIGIN      
        1 gcactgaaag gaattatttc ccctactccc ttatgtcaac tggaaaagta tggagacgtt
       61 tttcgttgac tcagaatccg agtttccaga atcttctggt aaatcatatt taacaaggga
      121 agaagcttct agccgagccc ttctgaattc agccatctta tagggagaat ttcctcggcg
      181 gagaaaatcg tcaaatgccc tttcttcaaa gtcttttcta gacacaagat attgtaaacg
      241 catagaaatc ttgcgcccgt tttcttgggt ttcttcgaac ataccaccga gcaatctaag
      301 atcctcaatc gcttttaatc gatctacaga aattttatgt tgattagatt ctccttgaga
      361 tacataggag ttttgaagca tagaattata gaatatctca agtgattttc cttttttctt
      421 tgcctccaat gccaagccat tcatgcctcc atctcctatc gaaaaaaaac gaacatcttt
      481 gctcgcaccc acttccacaa tacgcccgta aataccctct gcatcccatt caaagaaacc
      541 ctgtgctcca tccgcgctcg aaatagatac taatgtgaag ccttcactac gattttccca
      601 tgcctctaat tttggataaa attgtacaaa gccttccgga aaagatagag aatcactgct
      661 gcaagtcgtc aataaaaata taaaacaggc atataaacaa aaaatgagaa agctctctgg
      721 ccttttgttc ttgccagcaa aatcgaattt gctatagact ttgctcatag aactagcaat
      781 ccttgccgaa actatactat agagaagaaa tttatgacaa ttttatagca aatatccctg
      841 ctatgaaagc taggaagtga tataattaaa attggtttga tatagttttc gataaattaa
      901 ttagatatga aggaggcgtc ttttacaaag aaatcttgct tttcatgttt actcaatctt
      961 gatatagtta tatcattgaa gaaaagaccg aaatatccga ggagggtagt aatgaaattt
     1021 gtgaaaaggt ttctccctct gctggctata gtggcacttg tgctagcttc atgcgccacc
     1081 caagccggtc aggtaccaaa gggagcagaa actcccccgt ccgtagctga aagcgagcca
     1141 caacagcctc aagttcagcc agaggctgaa gttgaaaagc cttctgtaga gaaatctgag
     1201 ctgcaaagtc tattcgaaca agcaaatgat ctaaaagacg aggcaattaa attcggccta
     1261 gactcaatac tttccgacaa attccaagaa gcaaacaaaa aacacgatac tgtatccgca
     1321 aaatacaagg acctcgtcga agcaccagct caatacgatg gagcaaaggc atatcctcta
     1381 aaggacgaac ttgccgaagt cgtttcgaca tgggacgagc tcattcagga aggtaatctg
     1441 atatgcccag aaatggtcaa aatggagaga aatggagcag aactgatgcg ctccatagct
     1501 atggatgccg atgcgccaat gatggcctct gcccagtatg atatcggcct gcagtattgc
     1561 gctcaagctg aaatatatgc cgatgactcc cgctttttgg acgctattga ttcctacaaa
     1621 cttgcctctg ctgcattcga aaattcagct atagctgcag aagtcaataa gcttagagaa
     1681 aacatcgaaa ataatggcta ttcgagttac gaaagcatcc aaatgtactt agtcatgggc
     1741 gaagaaaaat acaaagaagc gcaggatcta tggcagaaag gcagtatcga ggatattgca
     1801 gcatctacct cagtgctaaa agaagccaaa gagtactatt atgaagtgaa ctccaagggt
     1861 tcaaatttta aggcactcga agccaaagac aaggctatag cagcccaaga tgaagctata
     1921 aaggtaaacg ctgacctaaa tgctcctgac gaatatcaaa atgcccttga tttatttgcg
     1981 gaagcagaga aaaacatgga agaggccgac tatatcagtg cctattccaa ctatatcgat
     2041 gcagctacag tcttcgaagc cgcacgcgat atttcgcaag ccatggcctt tgatgccgcc
     2101 agcgccattg cagaagccga agccagattt gcagaagcaa aagacagggc ttctgagctt
     2161 ggactggaag ataatattta tctgatcgaa gccagtctgc atctcgataa tgcaaaagct
     2221 cttgagaggg aattgaaata tagcgctgca atattcgaga ccaatgaggt atctaactat
     2281 ataggtttat cagattcctt cgtccagcaa gaagcagaga tacgcgaaaa ggctcgcctc
     2341 gatcagcttg agaaagacaa agctgcgtca gaaaaggcaa ttgcagacgc aaaagctcgc
     2401 atagcctggg ctgatcaagt ggaccttaaa tcggattatc caaagcaata tgaaagcgcc
     2461 tctaatgcta tggcagcagc cgaaaaggcc tatcaaatag agaaatatgc tgctgctaaa
     2521 acccttgcag aagaggtctc aacgactttg tccgatgatt tccaaaaaca agttctcgct
     2581 gctagggagc tagctaagat aaaggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnctacg atgcctctgt agcgcttgcc aatgatgttg tccgtatcat ctccgatgat
     2761 ctgatggcag aggtgaatgc cttgagagca gccgcagaac agcttgctgc agataaagca
     2821 atggccgagg aggtcatgcc aaaggcgcga gatcgcatgg tatgggctga tctaaacaac
     2881 atcaaagctg actattcagc ggtttacaat tctgcacatt ctgcgatgga agccgctgaa
     2941 aaagcttatc agttagagaa atatgctgct gctaccaagc tagccgatga ggtcctctca
     3001 acactttcgc ccgattttga ggccaaggtg gccgccgatc gtgccgaaaa agaaacccag
     3061 gaactcgtcg ctcaaaataa aaaatatgca gagacagcta tttctgatgc aaagagccgc
     3121 tatgattggg cagcctctaa gaatgcagct aacaactatc cagatctctt caaagaagga
     3181 ggagatttgc tagccgacgc ccaaaccgca ttcaatgctc tcgattatgt acgtgcaaag
     3241 gatctcgctg cacaggctta ttggaccctt atggaaattg gcgaatttgc tcctctgccg
     3301 gcaacatata aggtaaggct cattcccgaa cgcagagatt gcctctggag aattgctgag
     3361 tatccattcg tctacaataa tccatataag tggccagtcc tgtacgaggc gaacaagaag
     3421 actttcaagg atccttctaa tccgaatctc atattccccg atcaagtgtt aacaattcct
     3481 agcataaagg gagaagtgcg caagggagca tgggatccga aaaaaaccta tcaaccgctc
     3541 tctaagtaag tgcttagaca ataaaattgg gccgcccatt cgggcggcct tttttttata
     3601 ataaatctat ggagccggga tctagaaata gaatgcccta agataagcta tcatatccaa
     3661 gtggtcggag gcggcaatag tctctctaat ggcatgcatg gccagcaagg gatggcctac
     3721 atccactgta gggacgcctg ttcggcttgc cgtgatagga cctatcgtct ttcctggctg
     3781 aatatcggca cgggccatat agagttgggt ttttatacct gctttgctag ctatcgtatg
     3841 accaatttgc cgagaagtga aatctgttgc ataattcatg ttggcattgg attttagagc
     3901 aatacccctg cctaagagcg gcgtatagaa gtcatcaaat ttatccggat agccaggatg
     3961 ccatgcttgc gctccatcta ttgaaacaca ggctgaacaa gctagtgctc tatataaatc
     4021 ctgaccaaga ctgcgtgaag caagtgtaat tctggcaaga acatcacgca ggtaggagga
     4081 ttgagctcct ccaacagtca tcgaacctat ttcttctgca tcgaaaaaac atgcgacctg
     4141 cgttgatgct tgggcttgta cacttataaa agcttctatc aatgcggcac agccagcaag
     4201 atcatctagc cttggtgcat tgatgagcga attatcgaaa aagcgagaag gttcggtgtc
     4261 atagagccta agatcggatg agatgattgt tttagggtct actccgagct cttgagaaat
     4321 acgcatcatg atttctgaat aggaagcgct tgcagtctcg ttttcttttt cagattcgct
     4381 ttcggattca gactcttttc tattgagagt cgctaaacca aaaagagctg gcagttgctg
     4441 gtttagattg tattcaaaac ccttgttgat gtctcggttc aaatggatag caagatttgg
     4501 gatgactgca ataggttcat gacttatata aaagcgctgg tgtatatctt tgttttccag
     4561 cactgcgaga gccccagcca ctccaagcgg tctatcgagc caacctgata gaattggcga
     4621 gccataggat tccagcgcaa gccggagata gcccctagac tgaagttctt tttctggacg
     4681 aatcttgagc gcagggctat cggtatgggc tccagcgata agaaatccgg attcagaagg
     4741 tggttttaaa cccattctaa aagcaacaag agagcagctt cctcgaacta catagtaaga
     4801 aaccccgggc tcgagctgcc atatttctcg ctcatccaat ttaatggctc catgtgcttc
     4861 aagaatacct gtaaggttat ctatagcctg aaaaggtgtt ggtgatgcgt caacaaaatc
     4921 cattaagttc tttatcatct gttccatcca ataactcctt tcccaaattc ttctctaccg
     4981 aattttcctt taccgaactc tctgcggtag aaacagctat tagctctcct aatgaggctt
     5041 tccatcgatc acagtccaac aaaagacgaa atagttcttc cctcaattgc ttgatatttg
     5101 ctctaagctc ctgatcttga taaagaagct cgtactctag tattctctgt gcacgaccaa
     5161 gaccaagttt tttatccagc gctagatgct tgagccttgc aatgagaacc acttctgaga
     5221 gcgagtaatt tctgcggccg gatttcgttc tgctaggcat taaaagaggt acctcttgtt
     5281 cccagaaacg cagagtggac gcaggaatcg ataacattcg ctccaattgt ccaatgctca
     5341 ggtatatcat tgatcagctc cgcagtctgt ctctgcgtga agcacgcagt tccacctgaa
     5401 tcggaacacc atcgaggcca aaatcgaacc gtatcttgtt ttttaggaaa ctcttgtaag
     5461 attctggtac tgcctctggg cgcgttacga agaagataaa acgctgagga tttaccgaac
     5521 tctgcactgc atagcgcaat ttgaaacgag tgcttggtcc tgccggaggt ggagtctcct
     5581 gtatccattc cgcgaccgct tggtttaggc gcgaagtctc gataaccttg tttgccttag
     5641 catacaaaga gaccaccgta gacataagct tatcgagtcc ctttccttct ttagcggaaa
     5701 tcgccacgac gggagcatat gccatttgcc cgaaaaaaaa tcgcagccga tcgcgagtag
     5761 cttcgaacgc attctttata tcaggcatct tatcccattt gctcagcgca aacaccaccg
     5821 gtcggcccgc ttccaccgca aaagcggcaa tctttttatc ctggtcagaa agaccttctt
     5881 ctgcatctat gaggagcacc actacatccg cctggctcac aatgcggata gatcgggtga
     5941 ccgaataata ctcgacattg tctgtaactt tcttctttct tcggattcct gcggtatcga
     6001 gcacgacaat gcccctaccc ctatactgaa atcgatcttt tactatgtca cgagttgtcc
     6061 ctggctctgg agaaacaatg ctccgctcat agccaactag ggcattgagc aatgtcgatt
     6121 tcccagtgtt tggcttaccc attattgcta aatgtatgtc ggctttttct acttctactt
     6181 gctgaatgtt cgagaaatcc aagcgcgcca ctatggcttc tgcaagcttg tctatgtttc
     6241 tattatgttc cgccgagata aacaacatct gcccgaggcc ccagcgagca tgctcccata
     6301 ccagggcatc gcgctcggga ctatcggctt tgtttacgac aagcaaaagc tttttggcat
     6361 aaggcctaag aaatgcagcg aattcttcat cttccggtgt gatctgcgta atatctacaa
     6421 tgaatattat tagatctgct ctctgaatat aggctagagc tctagccact acaagctcat
     6481 ccaggccttc acgctcaagc ttgaagcctc cagtgtcgac taggcatatc cttttgttgg
     6541 tccccaatag ctcgcattct tcttcgatag gatctcgtgt tactccaggc ttcgcatcgg
     6601 ttattgcctt ccgcttgccg atcaatcgat tgaaaagcgt agatttgcct acattaggcc
     6661 tccctgcaag aaccacaaga ggcaggtttt catagcgaat gtttggtgaa gactcttctt
     6721 tctcttttga ttgtgataaa gcgccttttt gctgcaagtc tatctcaaga ggctctgcag
     6781 tctgtttttt tttgctcata agaataatta tagattgagc ctagaattca gcaaggcagt
     6841 aacctgcgat gccgtattca tggatagcgc ttgctgagcc agggaggaag cttcttccat
     6901 cgttatggac atcagcgctc tcttaaccgc tgggatagct gaggctgaca tagagagact
     6961 tcttaagccc aatcctacta ggagcaccgc gctagctgga tctgctgcca tctctccaca
     7021 gagactcacg ccgatatttg ccttcttact agattttatt gtcatactga taaggcgcaa
     7081 cactgctata tcgaacggat tattcaaata agctactttt tgattacccc tatcaacagc
     7141 cagcgtgtat tgacttagat cgtttgttcc tatcgagaag aaatcacaag atcttgagag
     7201 tac
//
LOCUS       DAOSOB010000077         6917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_11006_length_6917_cov_47.106237, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000077 DAOSOB010000000
VERSION     DAOSOB010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11006_length_6917_cov_47.106237"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="PLS88_07600"
     CDS             <1..986
                     /locus_tag="PLS88_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HOU07246.1"
                     /translation="HRSLRTEKAYLAWTHRFLNFVWSKKLVQEAKGKKGDYIITADHL
                     RAFLSYLALEAHVSAATQEQALNALLVLFRTILHVDIEGLSSVLRAKKRKRLPVVLSR
                     NEVAALLASLRQPYRLMACLMYGCGLRLEECLSLRVKDIDFDHDSIEVRSGKGGKDRL
                     TVLPEVLKGDLRAYLAELRQAWEQARRRDLPGVFLPEALARKYPSLSKEWSWYWLFSA
                     RSPCANTRTGEIGYWHVHPSVIQKKIKEAILQAGITKMASAHTLRHSFATHLLEDGYD
                     IRTIQELLGHSNVQTTMIYTHVAIKNRRGVCSPLEHLQGGTLSGQRSSLRR"
     gene            1391..2428
                     /locus_tag="PLS88_07605"
     CDS             1391..2428
                     /locus_tag="PLS88_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07247.1"
                     /translation="MSRKRNISIQRRVAKPNTYQFWRSLALFSIVSLLAACSPPSLEN
                     KMLGDIRPPIVRDAKLKNAGEFEIEFDETVCVGREAFSAEPKRVAVCAESSGEKVCIS
                     LLPQPFPGEKVILAGTVEDICGNSTHVQVQFKGYNDKPARIILTEVQTAKNTSKKAPH
                     RDYIEFIVQKGGNLGGMFVQWASSAKIMKYDFMPCEVRADEVIVLHLAPEGILEEKDE
                     TGEDTALSGGIDANAKGRDFWSEAGGLPDSSGAISVYEREGSLPIDGLFYGESSKSGP
                     LESSKLISIVQELSDARIWNSESPAKWENAFLWKPSASRPFIRTSLSQSGASAWKVGE
                     SGSQTPGSFSR"
     gene            complement(2421..2744)
                     /gene="trxA"
                     /locus_tag="PLS88_07610"
     CDS             complement(2421..2744)
                     /gene="trxA"
                     /locus_tag="PLS88_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOU07248.1"
                     /translation="MSAEVTLTAENFKKEVLESKIPVLVDFWAEWCMPCKMIGPSISQ
                     IAETYKDKIKVGKLNVDSEAEIASQYSIISIPTLIIFKDGQAIRQKVGAMPRHEIEKL
                     FSDLI"
     gene            complement(2935..3007)
                     /locus_tag="PLS88_07615"
     tRNA            complement(2935..3007)
                     /locus_tag="PLS88_07615"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2972..2974),aa:Asn,seq:gtt)
     gene            3292..3855
                     /locus_tag="PLS88_07620"
     CDS             3292..3855
                     /locus_tag="PLS88_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07249.1"
                     /translation="MQRVKIAITANEPVLYTEFYPIFEDSPFLIIIDEYNHVQKYSAE
                     IGAKGILKGRAEWIIGRGARILVTGSIENEDYQKLRRAGVAIKWESFGEVKSLVERAR
                     RFADYLLEAMENEKHVDRSRFDRRLRTMSIAAPYFGHSQEIDPRYLESLEQKAEKKGK
                     KLLLQRKDEEDSNDNDYETSGNTSHKQ"
     gene            complement(3874..5949)
                     /locus_tag="PLS88_07625"
     CDS             complement(3874..5949)
                     /locus_tag="PLS88_07625"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOU07250.1"
                     /translation="MAYINKLLTENFLYYASYVIMDRAIPELDDGLKPVQRRILHSLF
                     EMDDGKFHKVANVVGHCMKYHPHGDASIASALISLANRGLFIDTQGNFGNPITGDEAS
                     APRYIECRCSPFAKEIFYHPKITRYINSYDGRNKEPLVFPAKIPVILILGAEGIAVGM
                     STKILPHNPIEVIKAEIAYLQGKTSTIYPDFPTGGTADCSEYADGNGKVRVRATIDSS
                     DPKKIVIKEIPYGSTTESLIGSIEEAVRAGHLKIASISDFTTSKVEIELKLARGVYAQ
                     DVVDALYAFTECERSISCNCLVIKEGKPVITTVSETIEYHAGKLLILLRQELELEREE
                     LKNELFSRTLERIFIEEKIYTSIETKRTNDTVIKAVLDGLKPFTKELARKVEIEDVEQ
                     LLKIPIRRISLYDIEKAKKEMEQIERKISENSRHLSHLVDYAIEILQIYIEMIEKNCP
                     RKTKLKAFKKVEARVVARHDLALKYDAEGGYLGTEVSGEKLLEASKFDKILILRNNGI
                     YTIIPLPDKFFAGQKILWTAIATKESLSKITITLIYSLSNENGAYIKRTIIDSWLTGR
                     DYSLVPETAVILGFSTENEFDFKLEYKPKSRSKKTSEVFHVKQYAVRNRAAQGIKLTD
                     REVIKFEMLSSQKSETKREQKELAPSKSPVSKKKTNKKAKEKKETAPSELTSGGLLKA
                     AIQKQKKET"
     gene            complement(6001..>6917)
                     /locus_tag="PLS88_07630"
     CDS             complement(6001..>6917)
                     /locus_tag="PLS88_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOU07251.1"
                     /translation="ISIRIKNPIFESQTKNKLGNSDVRPWILQEVKKGVDEVLRRNPQ
                     AAKILQEKILTNEKLRTELNIVRKEAKEAAKKIELKIPNLKDCKIHLGDGDMGDQSTI
                     FITEGLSASGSMVSARNVHTQAIFSLRGKPENMFERPRSAIYKNEELYNLMMALGIEN
                     NVENLRFARIVIATDADYDGFHIRNLLLTFFLTFFEELVVQGRVYLLETPLFRVRTKK
                     ETLYCYSIHERDDAIKKLGQGSEVTRFKGLGEISPAEFGQFIGKNIRLVKVDIQTLSS
                     VPELLNFFMGKNTPQRRQFIMDNLVLDL"
BASE COUNT         1789 a         1378 c         1582 g         2168 t
ORIGIN      
        1 aacatcgctc actgcgcacc gaaaaggcat atcttgcatg gacccatcga tttctaaatt
       61 ttgtgtggag taagaagctt gtacaagagg cgaaaggcaa gaaaggcgac tatattatca
      121 ctgcagatca tctgcgcgcg tttttgagtt atcttgcgct tgaggcccat gtgagtgcag
      181 cgacacaaga gcaagcgctc aatgcgcttc ttgtgctgtt tcgcacgatt ttgcatgtcg
      241 atattgaggg actttcgagc gtattgaggg cgaaaaaacg caaaaggcta ccagtggtac
      301 tcagccgcaa tgaggtggcg gcattgctcg catcgctgcg tcagccttat cggcttatgg
      361 cttgccttat gtatgggtgc ggattacggc tcgaggaatg tctttccctc agggtgaagg
      421 atatcgattt tgatcatgat tcgatcgagg ttcgatcagg aaaagggggg aaggataggc
      481 tcacggtcct tccagaggtg ctcaagggtg atttgcgggc gtatcttgct gaattgcggc
      541 aagcttggga gcaagctaga aggcgagatc tgccaggggt gtttcttcct gaggcgcttg
      601 cacgaaaata tccttcgctt tcgaaggaat ggagttggta ttggctcttt tctgcacgaa
      661 gtccttgcgc aaatactcgt accggcgaaa ttggctattg gcatgtgcat ccttcggtga
      721 ttcagaagaa gattaaagaa gcaattctcc aagcgggtat cacgaagatg gcatcggcac
      781 ataccttgcg gcatagcttt gcgacgcatc tactggaaga tggttacgac atccgtacaa
      841 ttcaagagct tttgggacat tcaaatgtgc agactacgat gatttatact catgtagcga
      901 ttaagaatag acgcggcgtc tgcagtcctt tggaacatct gcaagggggc actctaagtg
      961 ggcaaaggag ctctttgcgg agataaaaca gtgtgtttta gaagataaaa tcgctcgtct
     1021 ttgataaggt aatgccttac tgtgcaattt ttctttaatt gagagaacat tctcttatat
     1081 atacaatatt cccaatgttg gagctcaacc caaaaatgag gatttttttc cgcgatgcta
     1141 agtgcgaccg ccgcaacagg ttcacatctt agtatatcgc gcttttataa ggttttacct
     1201 taagacctat tgtatgtttg ccttttagca cagtcaattg tccttcattt atatattgag
     1261 tttttctaga aattaatttt gaagcaatgt attggattgc tcgcgaaata tcatagaacc
     1321 tatcgatttg agctccttgg aaatgacgct ggaaaaagat gctcttttat atgcatggag
     1381 gcgagagaaa atgtcgcgaa aaaggaatat ctcgattcaa agaagggtag caaaaccgaa
     1441 tacctatcaa ttttggcgct ctttggcttt attctcgatt gtgtccttgc ttgcagcttg
     1501 ttcgcctcca tcgctggaga ataagatgct tggtgatatt cgtcctccta tagtgcgaga
     1561 tgctaaacta aaaaatgctg gcgaatttga aatagaattc gatgagactg tatgtgtggg
     1621 cagagaggct ttttccgctg agcccaaaag agttgcggta tgcgcagaga gttcagggga
     1681 aaaggtgtgt attagccttc ttcctcagcc ttttccagga gaaaaagtga ttcttgcggg
     1741 cactgttgaa gacatatgcg gcaatagcac tcatgttcag gttcaattca agggctataa
     1801 tgataagcct gcccgaatta tcttgaccga agtacagact gctaagaata cctcgaaaaa
     1861 ggctcctcat cgcgattata ttgaatttat cgtccagaag ggtgggaatc taggtgggat
     1921 gttcgtacaa tgggcaagct ctgcaaagat tatgaagtat gacttcatgc cttgcgaagt
     1981 aagggcagat gaggttattg tcttgcattt agcgccagaa ggtattctag aggaaaagga
     2041 cgaaacggga gaagatacag cactttctgg cggcatcgat gcaaacgcca agggaaggga
     2101 tttttggtcg gaagcaggcg gtttgcctga ttcgagcgga gctatctctg tatatgagcg
     2161 agaggggagt cttcccatcg atggattatt ctatggtgaa agctccaaga gtggtccttt
     2221 agagtcttca aagcttatct ctattgtaca ggagctttca gatgcgcgaa tatggaattc
     2281 tgaatctcct gcgaaatggg aaaatgcatt tctatggaaa ccttcggcat ctcgaccttt
     2341 tatccgaaca tcactatctc aatctggggc aagtgcgtgg aaagtcggag aaagcggtag
     2401 tcaaacgcct ggctcttttt ctagataagg tcgctgaata gtttttctat ttcgtgccgg
     2461 ggcatggcgc cgactttttg gcgtattgct tgtccgtctt tgaatattat tagcgttgga
     2521 atagaaatta tgctgtattg agaagcaatt tcagcttcgc tatctacatt gagttttccc
     2581 accttaatct tatctttata agtttctgcg atttgggaaa tagatggtcc tatcattttg
     2641 cagggcatgc accattcggc ccaaaaatcg acaagtacgg gaattttcga ctctagcact
     2701 tcttttttga aattttcagc ggtcagggtt acttcggcgc tcatctttgc tcatgctcct
     2761 tcgaataatt ggctttctct atgtattgtt catagggaat gctaatataa aaatataatc
     2821 acccttgtca taaagagtca aattcgaaaa attcatgata tgcacagatt gagcctaaag
     2881 tggggctaat cttataaaaa aggccatcca taaggatggc cttaagctca ttttctcccc
     2941 cgcggtggtt cgaacaccgg acccagtggt taacagccac ttgctctgcc agctgagcta
     3001 caggggaata tgaattctca gcggaaagta aaatagccaa aattagcact tttgtcaaga
     3061 ttatgtgttg ccttcgatta cctttccata aaaatttcag gcgcctttga agcatgctgg
     3121 catacgattt aagaatcaaa agtgcactat tttttgagga tttaaagctt aaaagtgcat
     3181 tatcgattct tagttaaccg attaaatcga ttgcttatat gaaatatctg cgagtttata
     3241 cttgacccct ctatatgcaa agagtaagaa tgattcaaaa gggttctatc catgcaaaga
     3301 gtcaaaattg ctattaccgc caatgagcca gttctgtata cagagttcta tcctattttt
     3361 gaagacagcc ctttcctaat aattatcgat gaatataacc atgtccaaaa gtattctgct
     3421 gagattggcg caaaagggat tctaaaaggt agagcggagt ggataattgg cagaggcgca
     3481 agaattcttg tcacaggatc tatcgagaac gaggattatc aaaaactgag gagggctggt
     3541 gtagccataa aatgggagtc ttttggcgag gtaaaatcct tggttgagag agcaagaaga
     3601 tttgctgact atctgctaga ggcaatggaa aatgagaagc atgtagatag gagcagattt
     3661 gacaggcgcc taagaacaat gagcatcgcc gctccatatt ttggtcactc tcaggaaata
     3721 gatcctagat atttagaaag cttagaacaa aaggccgaaa agaaaggcaa gaagctactt
     3781 ttgcaacgca aagacgagga agactctaat gacaatgatt atgaaacgag tgggaatact
     3841 tctcataaac agtgagatag cgatctagca ttcttaggtt tcttttttct gcttttgtat
     3901 ggcggcctta agcaagcctc cagaagttag ctccgaggga gcggtttcct ttttctcttt
     3961 tgctttttta tttgtctttt tcttggatac aggagattta gaaggagcca attctttttg
     4021 ctctcgctta gtttctgact tctgagagga aagcatttca aacttgatta cttctcgatc
     4081 cgtaagcttt attccctgtg ctgctctatt cctcaccgca tattgcttga catgaaatac
     4141 ctcagaggtc ttcttgctcc ttgatttagg cttgtattct aacttaaagt cgaattcatt
     4201 ctcagtcgaa aatccgagaa tcactgctgt ctctggtacc agactatagt ccctgccagt
     4261 aagccagcta tcgattatgg ttctttttat atacgctccg ttttcattgg ataatgaata
     4321 gataagcgtt atcgtgattt tggagaggct ttcctttgtt gcgatagctg tccacagaat
     4381 tttttgcccg gcaaagaatt tatcgggaag agggataatc gtatatattc cgttgtttct
     4441 caaaatgaga atcttgtcga atttagaggc ttcaagcaat ttttcgcctg aaacttctgt
     4501 tcctagataa cctccttcgg cgtcatattt gagtgcaaga tcatgtcgcg cgacaaccct
     4561 ggcctctact tttttgaatg cttttagctt ggttttacga gggcaattct tttcgatcat
     4621 ttcgatataa atctgcagta tttcaattgc gtaatctact agatgactca aatgccttga
     4681 attctcggat atctttcttt cgatctgctc catttctttt ttggcttttt ctatgtcata
     4741 gagagatatc ctgcgaatgg gtattttgag gagctgctcc acatcttcta tttcgacctt
     4801 tctagcaagc tcttttgtaa atggtttgag tccatctagg acagccttaa tcacggtatc
     4861 attagttctc tttgtttcga tcgatgtata gattttttct tcgatgaaaa tgcgttcgag
     4921 cgtcctagag aagagttcat tctttagctc ttctctttct agttcgagct cttggcgcag
     4981 cagaatcagc agctttcctg catggtattc tatagtctcg gatacagtgg taattactgg
     5041 ctttccttct ttgatcacta ggcagttgca ggaaatcgat cgctcgcatt ccgtgaaggc
     5101 atagagcgca tctactacat cctgcgcata cactcctctc gccagcttta gctctatctc
     5161 aaccttgcta gttgtgaaat cggagataga agcgattttg agatgaccag ccctgactgc
     5221 ttcttcgata ctgcctatta agctctcggt tgtagaacca taagggatct cttttataac
     5281 gatttttttg gggtccgagc tgtcgattgt ggctcggact cttacttttc catttccatc
     5341 tgcatattcg ctacaatcgg cagttcctcc tgttggaaaa tctggataaa tagtggaggt
     5401 ttttccttga agatatgcta tttcggcttt gattacttcg ataggattgt gtggcagtat
     5461 ctttgtgctc attcccactg caatgccctc cgctcccaag atcaaaatta cagggatttt
     5521 tgcagggaat acgagcggtt ctttatttct tccgtcatag ctgtttatat agcgagtgat
     5581 tttaggatga tagaagatct ctttggcaaa cggagaacat ctgcattcta tatagcgggg
     5641 tgcagaagct tcgtctcctg ttatggggtt tccgaaattt ccctgggtat ctatgaacaa
     5701 gcctctgtta gcaaggctta tgagagcaga agcgatagaa gcgtctccat gagggtggta
     5761 tttcatgcag tggcctacga catttgcgac tttatgaaat ttgccatcgt ccatttcgaa
     5821 gagagagtgg aggattcgac gctgtactgg tttaaggcca tcgtccagtt ctgggattgc
     5881 tctgtccata attacgtatg aggcataata gaggaagttt tcggtgagca acttatttat
     5941 ataagccact ttattctcta tcctttcata gctttctggc tgattttttg gttttgggcc
     6001 tcaaaggtcc aagactaggt tgtccattat gaattgtcgc ctctgtggtg tatttttccc
     6061 cataaagaaa tttagaagct ctgggacaga tgataatgtc tggatatcga cctttaccag
     6121 tcgtatgttt ttcccaatga attgtccgaa ttctgcaggg cttatctcgc ctaaaccttt
     6181 gaaacgtgtt acttctgatc cttgcccaag cttttttatt gcatcatctc gttcatgaat
     6241 tgaatagcaa tatagagtct ccttttttgt tcttactcta aaaagtggtg tttcaagcag
     6301 atagactcgt ccctggacta ctaactcttc aaagaaggtc aagaagaatg tcaaaagaag
     6361 gtttcgtata tgaaaaccat cgtaatctgc atctgtagcg ataacaattc gggcaaagcg
     6421 caaattttcg acattgtttt cgatgccaag cgccatcata agattgtata gctcttcatt
     6481 cttgtagata gcagagcgag ggcgctcgaa catattttcg ggtttgccgc gcaagctaaa
     6541 aatggcttga gtgtgtacat tccttgcgct taccatgctt ccagacgcag agaggccttc
     6601 ggtaatgaat atcgtcgatt ggtctcccat atctccgtct ccaaggtgta tcttacaatc
     6661 cttgagattg ggtattttga gctcgatctt ctttgcagct tcttttgctt cttttcgaac
     6721 gatatttagt tcggtacgga gtttttcgtt agtaaggatc ttttcttgaa ggatttttgc
     6781 cgcttgagga ttgcgtcgca gtacttcatc tactcccttc ttgacttcct ggagtatcca
     6841 tgggcggaca tcgctgttgc caagcttgtt tttggtctga ctttcgaaaa tcggattctt
     6901 gattctaatg gagatag
//
LOCUS       DAOSOB010000078         6794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_11263_length_6794_cov_73.394717, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000078 DAOSOB010000000
VERSION     DAOSOB010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11263_length_6794_cov_73.394717"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1111
                     /locus_tag="PLS88_07635"
     CDS             119..1111
                     /locus_tag="PLS88_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU07252.1"
                     /translation="MWPIIVVYAGLVCTLTAVSYVLMFRPKCKSLEFTKQSSLERGEF
                     DESFLNLDWQEWSFASPHGYTLRGQYLRGRKDAPAALFVHGITWSRYGMVKYMRPFIE
                     RGWNVAAFDLAGHGASMAPRRFYPSYGFYEKYDVKAGVESLHSLFSNAPLYGLFGESL
                     GAASALQYAPLTKAKPSREVDFIVADCSFSSAWEELLEQYRAIHMPNFIAWPSAHVTR
                     LFVRLLRGFDIKEASPSKAVLLTDIPILFAHGMEDSYVPTIMSVRMASARLSNNIGLT
                     ELVLIPGARHAVGILTDQMRWLSAVDAFIDRVFLEKSEKCNKASCNISLNEESK"
     gene            1137..1541
                     /locus_tag="PLS88_07640"
     CDS             1137..1541
                     /locus_tag="PLS88_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOU07253.1"
                     /translation="MELKEGIIAEKEIIVEEKYVASHLGSGGVPVYATPMMVLHMEET
                     SRLVVDHLLESGKATVGAFIAVKHLAPTPKGMKVRIRSELAKIEGRMLTFKVEAWDEV
                     EKVGEAEHIRSIIDIERFSERIEKKKAIAASK"
     gene            complement(1552..2340)
                     /locus_tag="PLS88_07645"
     CDS             complement(1552..2340)
                     /locus_tag="PLS88_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07254.1"
                     /translation="MDYEKRLYMIVHPNHSLIASQLEPERFIKHYVQGSTRYFEGRLI
                     FVEVDPEFRNPYFDIDTAYSELKPHEDGRPKATKFIKSYRVLEHMDYNAFGKLYISNS
                     LGDYVELESKPHEPSIDSDEFRIMLEVNPVRFIVLTKLNYHEFGQFITDPRNTKGAPK
                     TAFTLLEFDSQQFMKEYEENPLIRCYVPGIHPARLAAAIEEVRNTPGKVVKGISLDCP
                     IDHISYKYLKEGFVFAEHDKPCKFYPYLDLGTIERRFYRFWKSM"
     gene            complement(2429..4504)
                     /locus_tag="PLS88_07650"
     CDS             complement(2429..4504)
                     /locus_tag="PLS88_07650"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOU07255.1"
                     /translation="MLSSGVFVAIITALAALVYSLITSSKIKKISLSNPTIIRISGYI
                     EEGAHAFLVREYKAVGFIIPVVAVLLLCLNNGALKLQAVSFLVGSVLSALSGWFGMGI
                     AVKANARTAEAAEKGVLPALRIAFSSGSVMGMTVVGLVLLGIGAILGLLLLMPEAEQI
                     LAQTIFPIVSGFSLGASMMALFARVGGGIYTKAADVGADLVGKVEVGIPEDDHRNPAV
                     IADNVGDNVGDVAGMGADLFESYAGSIVGCMVLGIAISASPELQQRLAILPLIAAGTG
                     VVASLIGTLFVHLSRAKHPQNALNAGSIGASIIAIIILIPVVRFIIGDGRFSIGSREA
                     GWLGVYLACITGLVAGTAIGLITEYYTGSDSPPVRKIAKSCTTGAATTIISGLGVGMI
                     STVFPILVIGLAIGLSNWLAGLYGIGIAGLGMLLTLGIQLSVDAYGPVADNAGGLAEM
                     AEMEPEVRRITDSLDAVGNTTAAIGKGFAIGSALLTSLILIVAFLSAAGLPVEMMNLA
                     NPSVLIGLFIGAMIPFFFSSLAMDAIYRAAFAMIEEVRKQFHAKPGILEGTDEPDYKS
                     CVDISTQTALKSMILPGISAIAAPILTGFIGGPAVLTGLLVGATVSGGLLAIFMSNAG
                     GAWDNAKKLIESRSEEGKGTSAHRAAVVGDTVGDPFKDTAGPSLNILIKLMAVISLVL
                     APVLKTVWG"
     gene            4977..6026
                     /gene="argC"
                     /locus_tag="PLS88_07655"
     CDS             4977..6026
                     /gene="argC"
                     /locus_tag="PLS88_07655"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOU07256.1"
                     /translation="MIHVGILGATGYAGLELTRILLQHPEIAALSASSVSFEGKRLED
                     VYPAMINIQSDVALPMLEKAEDVICKADIVFSCLPHGHAEEAGQQCFEASKPFIDISA
                     DFRFGIHENIFEKWYGAPYKYPKLHEYAIYGLPELNRKAIKSAVLIANPGCYPTSAEL
                     GLMPAIQESLVDISSIVITSMSGVTGAGREPSQTTHYPEAADAASPYKVGSHRHQPEI
                     DLLLSEMAGEQVQSVFTPILAPMNRGIVSTICFRLKRVVSDEELHSIYIERYVDEPFV
                     RVLPLGAAASNRFVKFSNYCDISVHLSADKERAVIISTIDNMVKGAAGQAVQNMNILL
                     GFEETEGLRFIPPAF"
     gene            6041..>6794
                     /locus_tag="PLS88_07660"
     CDS             6041..>6794
                     /locus_tag="PLS88_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOU07257.1"
                     /translation="MKQSRGGVTAAKGFLASGVHVGIRKNKEKKDLALIWSEVPCAAA
                     AVYTTNRVKGQTLIVTQEHLADGRAQAIIVNSGNANTCTGEEGLKAARRMAELVSQYL
                     PVKTEDVVVASTGVIGQQLDVSVIEAGMPDLVKGLSKGGSIDAREAIMTTDTMKKEIA
                     VIEELDGVPVTIGAMAKGSGMIHPNMATMLCFITTDCAIAEPLLKKALKEVVRVSFNR
                     VSVDGDTSTNDMVVILANGKANNAYIDKEDDNY"
BASE COUNT         1847 a         1586 c         1625 g         1736 t
ORIGIN      
        1 tgtgcctttg agctcgattt ttaacttggc atatagttgc ttagatttgc ttagagttaa
       61 gccctggcag taatttatag ccctgctcag ctcattaaaa atgcgctaga atgcaagcat
      121 gtggccgata atcgtagttt atgctggatt ggtatgcact cttacagcgg tgtcctatgt
      181 gcttatgttt aggccaaaat gcaagagcct tgaatttacg aagcaatctt cactcgagag
      241 gggggagttc gatgagagtt ttttaaatct cgattggcaa gaatggtcat tcgcctcgcc
      301 tcatggctat actctgagag gccagtatct acgaggccga aaagatgcac cggcagcgct
      361 ctttgtccat ggcataacat ggtcgcgtta tggaatggta aaatatatgc gaccattcat
      421 agagcgcggc tggaatgtag cggctttcga tcttgcaggg catggagcca gcatggctcc
      481 aaggcgcttc tatccatcct atggtttcta tgaaaaatac gatgtgaagg ctggagtcga
      541 atcgctacat tcgctctttt caaatgctcc actctatggt ctttttggtg aatcgctcgg
      601 agcggcctcg gcccttcaat atgcacctct cacaaaagcc aaaccctcga gagaagtcga
      661 ttttattgta gccgactgtt ctttttcttc ggcatgggag gagctcctcg aacagtatag
      721 agcaattcat atgccaaact tcatagcatg gccttctgct catgttacaa ggttatttgt
      781 acgccttctg cgaggcttcg acataaaaga agcaagcccg tccaaggcag tattgctgac
      841 cgatataccc attctctttg ctcacggtat ggaggacagc tatgtgccga ccataatgag
      901 cgtgcgtatg gcgagcgctc ggctgtcgaa caatatcggg ttgacggagc tagttcttat
      961 tcctggtgcc aggcatgcgg tgggaattct gacagaccag atgcgttggt tatctgctgt
     1021 agacgctttc atagatcgtg tctttcttga aaagagcgaa aaatgcaata aagcgtcgtg
     1081 caatatatct ttgaatgaag agagcaaata gaaatctgac catgaggaga gcagcaatgg
     1141 aattgaagga agggattatc gcagaaaagg aaataattgt ggaagaaaag tatgtcgcct
     1201 cgcatctggg ctcaggaggc gtccctgtct atgcgacacc gatgatggta ctgcacatgg
     1261 aagagacttc gcgtcttgtg gtggatcatc ttctggaatc aggcaaggct acggtcgggg
     1321 cttttattgc cgtcaaacat ctcgctccga ccccaaaagg aatgaaagtt cgaattcgct
     1381 ctgaactagc aaaaatcgaa gggcgcatgc taactttcaa ggtcgaagct tgggacgaag
     1441 tagaaaaagt cggagaggca gaacatattc gttccattat agatattgaa cgattttccg
     1501 aacgaatcga gaagaaaaaa gctatagccg cttcgaaatg agcgctatat attacatgct
     1561 cttccagaat ctatagaatc tacgctcaat agtgccaagg tctagatagg ggtagaattt
     1621 gcatggttta tcatgctcag cgaagacgaa tccttccttg agatatttat acgaaatatg
     1681 gtcgattgga cagtctagag aaataccctt gacaaccttg cctggcgtat tgcggacttc
     1741 ctctatggcc gctgcaagtc ttgcgggatg tataccaggt acatagcagc ggataagcgg
     1801 attttcctca tattctttca tgaattgttg actatcgaac tcgaggagtg taaaagcggt
     1861 ctttggcgca cccttggtgt tgcgagggtc ggttatgaat tggccaaact catggtagtt
     1921 tagttttgtt agcactatga atcgcacggg atttacttca agcattatcc taaattcatc
     1981 gctgtctatt gaaggttcgt ggggtttgga ttccagctcc acataatctc ctagcgaatt
     2041 gcttatatag agcttgccga atgcgttgta atccatatgc tccaataccc ggtagctctt
     2101 tatgaatttt gtggcctttg gtctaccgtc ttcatgaggt ttgagctctg aataagctgt
     2161 atcgatgtcg aaataggggt ttctgaattc aggatcgact tcgacaaaga tcagcctacc
     2221 ttcaaagtat cgcgtcgagc cctggacata atgctttatg aagcgttcag gctcgagctg
     2281 tgaagctatt agcgagtgat tcggatgaac gatcatatat aaacgttttt cataatccat
     2341 ggggcgaacc tcctaaggtt ccgaatagcc taaatatatt atcgcaattc ttacatattt
     2401 aaagaaattt ctgcaatagg ctaaaacatc aaccccacac tgtttttaga acaggagcaa
     2461 gtacaagcga aataaccgcc atcaatttta tcaggatatt tagcgaaggg ccggccgtat
     2521 ccttgaaggg atcgcctacg gtatctccca ctacggcagc acggtgagcg ctagtgccct
     2581 tgccttcttc gcttcgtgat tctatgagtt tcttggcatt gtcccaggct ccgcctgcat
     2641 tcgacatgaa tatggcaaga aggcctccgg agaccgtggc gccaactaat aatccggtaa
     2701 gtacggcagg cccaccgatg aagccagtca atataggcgc ggctatggcc gaaatacctg
     2761 gcaatatcat gctcttcaaa gctgtctgtg tagaaatgtc gacacagctt ttatagtcag
     2821 gttcatcggt tccttcgaga atgcctggtt ttgcgtggaa ctgttttctt acctcttcta
     2881 tcatggcgaa tgcagcccga tagattgcat ccatggccaa tgaactgaaa aagaaaggaa
     2941 tcattgcgcc aatgaaaaga ccgattaaca cgctgggatt tgcaagattc atcatctcta
     3001 caggcaatcc tgcagcgctc aaaaaggcaa caatcaggat gagcgaagta agaagcgccg
     3061 atccaatggc aaagccctta ccaatggccg ccgtcgtatt accgacagcg tcgagcgaat
     3121 ctgtgattct tcgtacttct ggctccattt ctgccatctc cgcaagaccg cccgcattat
     3181 ctgcaacagg accatatgca tcgaccgaga gttgtattcc gagtgtgagc agcattccga
     3241 ggccagcaat cccaatacca tagagccccg caagccagtt tgagagccca atggctagcc
     3301 caatgaccag aataggaaat accgtagaaa tcatgccgac gcctaagcca gagatgatcg
     3361 tggttgcggc gccggtagta caggattttg ctatctttcg aacaggagga gaatccgagc
     3421 ccgtgtagta ttcggttatg agaccaattg ccgtacctgc caccaagcct gttatgcatg
     3481 caagatagac cccaagccac cctgcttctc tcgacccaat cgaaaaacgg ccatctccaa
     3541 taatgaagcg aacgactgga ataagaataa ttatggctat tatagatgct cctatagaac
     3601 cggcattgag ggcattctga gggtgtttgg ctcggcttag gtgtacgaag agcgttccga
     3661 tgagcgatgc caccacacct gttcccgcgg cgatgagcgg caatatagct aatctttgct
     3721 gaagctctgg gctagcggaa atcgcaatgc cgagtaccat gcagccgact atcgatcccg
     3781 catacgattc gaaaaggtct gctcccattc cagcaacgtc gcccacatta tcgccaacat
     3841 tatccgcaat gactgcaggg tttcggtggt catcttcggg tataccgact tcgaccttgc
     3901 ctacgaggtc ggcaccaaca tcggcggctt ttgtgtaaat gccgccgcca acgcgtgcaa
     3961 aaagagccat catcgaggcc cctagcgaaa atccagacac gatcgggaat attgtttggg
     4021 ccaatatctg ctctgcctct ggcattagaa gaagaaggcc taggatagct cctattccca
     4081 agagcacaag gccaaccact gtcataccca taacagatcc gctcgaaaac gcaattctga
     4141 gtgcaggcag gacacctttc tccgcagcct ccgcagtcct agcattggcc ttgaccgcta
     4201 tgcccatacc aaaccagccc gacagcgcgg acagaactga gccgaccaga aaagaaacgg
     4261 cttgcaactt aagagcgcca ttattgagac aaagaaggag aacagcaacc actggaatga
     4321 taaagcctac tgctttgtat tccctgacga ggaatgcatg ggctccttcc tctatatagc
     4381 ccgaaattcg tattatagta ggattggaaa gagatatctt tttaattttt gagctcgtaa
     4441 tcaaggaata caccagcgcc gccagggctg ttatgattgc aacgaatact ccactcgaga
     4501 gcatcggcga cctcctaatt gcgaaaagcc aaattgtgct ttacgtttca ttataaggct
     4561 ggttatttaa atctggcaat attgaaaata aatacaaaac ccctagcaaa aacatgataa
     4621 tgcctagaaa tattgagaac ataataacta tgtacagaga gcacggcagt attatgcatt
     4681 atatgtcggg tttaagaaaa ttttgtggaa agttaattaa tctattgcgc atctatatcc
     4741 ttgacaaaaa cagcgaattg tatttttatt gaaacacttt tttcttggga ggggcacatg
     4801 gaatgctcgc ttctcatatc tatctactca ccatctctaa gaactctgcc aaagcgcttt
     4861 cttgcattcc attctcgtcg tcttcatcta ttctgctatc gtcgttgaca atcttgcact
     4921 ttatacgtgc aatttacctc atttctcatt acacttgtta ttgaaaggag atgcccgtga
     4981 ttcatgtggg tatattaggg gccaccggct atgctgggct cgagctgacc cgaatcctct
     5041 tgcagcatcc ggaaatagca gcattgagcg cttcatctgt gagctttgaa ggcaaaagac
     5101 tcgaagacgt ctacccagcg atgataaaca ttcagagcga tgttgcgctt ccaatgcttg
     5161 aaaaggccga ggatgttata tgtaaagccg atatcgtttt ctcttgctta ccgcatgggc
     5221 atgcagagga agcaggtcaa caatgcttcg aagcatcgaa gccttttatc gatatctctg
     5281 ccgattttag gtttggaatc catgagaata tattcgaaaa atggtatggt gcgccgtata
     5341 aatatccaaa actgcatgag tatgcgatct atgggctacc cgagttgaac cgcaaagcaa
     5401 tcaaatcggc tgttctaatc gcaaacccag gatgctaccc tacctctgcg gagctaggcc
     5461 ttatgccagc tattcaggaa agccttgtgg acatttccag tatcgtgatt acttcgatgt
     5521 cgggagttac tggggcaggc cgcgaaccga gccaaacaac gcattatcca gaagcagcgg
     5581 atgctgcttc gccctataaa gtcggctctc ataggcatca gccagagatt gatcttttgc
     5641 tctctgagat ggccggagaa caagtgcaga gcgtattcac accgatcctc gctccaatga
     5701 atcgaggaat tgtgagcacg atctgctttc gacttaagcg tgtagtctcg gacgaggagc
     5761 ttcattcgat ctatatagag cgctatgttg acgaaccctt tgtgcgagtg ctgccacttg
     5821 gagctgcggc ttcgaaccgt tttgtaaaat tttcgaacta ttgcgatatc tcggttcacc
     5881 tatcagcgga taaggagcga gccgtcataa tctctactat cgataatatg gtaaaaggcg
     5941 cggctggtca ggccgttcag aatatgaata ttctcctggg cttcgaggaa acagaggggc
     6001 tcaggttcat tccacctgca ttctaggcaa ggagggtgca atgaaacaaa gtcggggcgg
     6061 tgtaactgcc gcaaaaggat tcctagcttc tggcgttcat gtcggaattc gcaaaaacaa
     6121 ggaaaagaag gatctagccc tgatatggtc ggaagttccc tgtgcggctg ctgctgtgta
     6181 cacgacgaat agagtaaagg gtcagactct tatcgttact caggaacacc tggcagatgg
     6241 ccgcgctcag gctataattg tcaactcggg caacgcaaac acctgtaccg gagaagaagg
     6301 tctaaaagct gcacggcgca tggctgagct tgtatcccag tatcttccag taaagaccga
     6361 agacgtagtc gtagcctcta ccggtgtcat tggtcaacag ctcgatgtgt cagttataga
     6421 agccggcatg cccgatctag taaaaggcct ttccaaaggc ggatcgatcg atgccagaga
     6481 ggcaatcatg acgaccgaca caatgaaaaa ggaaattgcg gtcatcgaag aactcgatgg
     6541 cgtaccggtt accataggag ctatggccaa aggttccggc atgatccacc caaacatggc
     6601 aacaatgctc tgctttataa ccacagactg tgcgattgcc gaaccgcttc ttaagaaagc
     6661 gctcaaagaa gtcgtccggg ttagcttcaa ccgcgtcagt gtagatggcg atacttccac
     6721 taacgatatg gtcgtaatcc ttgccaatgg caaagctaac aatgcgtata tcgacaagga
     6781 agatgacaac taca
//
LOCUS       DAOSOB010000079         6476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_12020_length_6476_cov_75.161969, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000079 DAOSOB010000000
VERSION     DAOSOB010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12020_length_6476_cov_75.161969"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..1205)
                     /gene="murB"
                     /locus_tag="PLS88_07665"
     CDS             complement(258..1205)
                     /gene="murB"
                     /locus_tag="PLS88_07665"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOU07258.1"
                     /translation="MKKLWQIAQKINTDHCSISFDEPMKEHTTFRIGGPADLFIRPRS
                     ILAFKEVLELLQIENVPFFILGGGANILVGDKGIRGAVIDTSLLSSIMHLKQKNKNDP
                     ILLYAECGVPLITLCEEAQVCGLSGLENFYGMPGSLGGSIYMNARCYEDDISTHIQDL
                     TIIDNQGRFKILPASAQSWSYKRSPFMPGGELESFIICAASFRLISDDPKKIAERMCF
                     RMRDRMQKRHFNFPSAGSMFKNNHDFGKPTGKILDELGLRNYRVGNALISPWHANIFV
                     NIGEASARDMKELIEYAQKVVHNATGFCLEPEVLFVGEF"
     gene            1272..2699
                     /gene="cysS"
                     /locus_tag="PLS88_07670"
     CDS             1272..2699
                     /gene="cysS"
                     /locus_tag="PLS88_07670"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOU07259.1"
                     /translation="MHDVYIFNTMGRNIQLFKPLHEGSVGFYGCGPTVYNYAHIGNLR
                     TYIFEDTLVRMLKRFGYEVTHVMNITDVGHLSSDDDTGEDKMLKSARERGQSVLEIAQ
                     FYTDAFFRDTERLNIQQPKIICKATDHIQDMIELIKRIEANGYTYFAGGNLYFDVSKF
                     DRYGELAGLQLDQLKAGARVELDPNKRNPFDFVLWFTKSKFDKQALLWESPWGIGYPG
                     WHIECSAMSMKYLGEQFDIHAGGVDHIAVHHTNEIAQSEAATGHKWVNYWMHAEFLVI
                     DKGKMSKSSGSFLTLESLIDRGYDPLDYRYFCLGGHYRSQLSFSWEGLDQAKSARLSL
                     VDKVLALKEKVAKPAALSELGEKALQYRSAFDEALADDLAMPRAMAQVWNLLKDNDVP
                     AQDALALILDMDEVLGLDLAKAQRKEVSVDPETLQWINSQIERRAAAKKARDFATADA
                     IRSELIAKGIQLEDRPEGTVWRLHE"
     gene            2736..3332
                     /locus_tag="PLS88_07675"
     CDS             2736..3332
                     /locus_tag="PLS88_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU07260.1"
                     /translation="MFQNKDDGPISNYNLIFAQNVDLLLRIAVHITNDWESAEDIVQD
                     AFSKLIEKQMRFPSEEDARYWLIRVVKNGAINWSRRKVREFKAYEHWWNTETTQAKIS
                     ADALDSRDMQNQHAGTDQSADHEILMKECAQEVRDALMKIPEKLRIVLILKEYEGMNY
                     KEIAKVLGITEGNVKVRAFRARETLLSILNEGGSYVSR"
     gene            3319..3945
                     /locus_tag="PLS88_07680"
     CDS             3319..3945
                     /locus_tag="PLS88_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOU07261.1"
                     /translation="MCPDESLLTAYVDNEVPSPWKERIEMHLGQCERCANRVAQYRAL
                     KLALKAADVFDAPQREEAAKRIQASLRSITDESSHSLRTEAIFERYPLFSALMSRRIS
                     VPLPVLAASLLITVFFAGLAFGIFGAHRTTGQALALSTKLPEESASNIESIVSLLSQT
                     DPNQFVTIHAPGNISSKPLVDSTPVYVIYNPSGRKPTIMAFPVQGEIK"
     gene            3942..4505
                     /locus_tag="PLS88_07685"
     CDS             3942..4505
                     /locus_tag="PLS88_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07262.1"
                     /translation="MKRSALALAAFSILNIAWLFAQTPSIPTAPSSIVAPDSSSSNIP
                     IFQFQIRTAFTLTPGMVAAIQSASPGSLGSKAGQGESIDKANSPNWEDKVVREVSLAS
                     PLGIKIENDRLTAIIVFMPIELVKKDITMLVQNHIYMRSPDNSIQLNTSVHTVRIPVD
                     SVFYYYPLGGDSKQGAPMVIEILVNSK"
     gene            4510..6144
                     /locus_tag="PLS88_07690"
     CDS             4510..6144
                     /locus_tag="PLS88_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOU07263.1"
                     /translation="MKQRRIITLCILILLVVTGIIVYLRIQPRLLAIDPVTPMPGDLV
                     NIVGHNLGKNGGELLLDNVPLPSQAVILWSPVLISFRMPSNYDSATVQARTKLGVSNP
                     LMIALASKVPAKAENNIVQQQPPSITSIKPSTNAQIGKLIELRGSYFGMPEDESAVFF
                     SKVPIISAVEAVDSDNFIRVESMSLFIDKWNSSYISAYVPDGVENGYVLVRTRNGLSN
                     AFPISVSRNPGRLSRGDSVTYTLEDTLVIHVAASLPDGRVSFILSKPPTTQYQSTITT
                     IETGSDHLRAETAVWQEFRFGAADARLGRIELRRRLVIDVCEIKADIAIGSLQPLGAK
                     PPAFLSSYLAADALVPSANPAIVSAALSIQKKEKNPFRQIVLAAQWTFSNIKLNEEKQ
                     ILRDDAINALKSKAGGMRSLVLINCALLRALGIPAIPVAGFLVTEDMRLIPHYWSEYY
                     LAGIGWIPFDPALAIGITPSGFRPIFTDRSSYYQGIDNRHIAMARGYQLLPSVPVEAQ
                     SKTKISWSLFEYDEAAYGLSYTSTWNPPVLLSTAIQ"
     gene            6230..>6476
                     /locus_tag="PLS88_07695"
     CDS             6230..>6476
                     /locus_tag="PLS88_07695"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307286.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOU07264.1"
                     /translation="MSYIKSMDPELYAAMEAEAERQQEKIELIASENYTSKAVMEAVG
                     SVLTNKYAEGYPGKRYYGGCEFVDIAENLARDRAKKLF"
BASE COUNT         1867 a         1407 c         1460 g         1742 t
ORIGIN      
        1 tgcagcgata acgaggtaca ttgccaaaac atttggtatg atagaagcct agcctgacaa
       61 accagcgacc ttttggagct gcctcatgat agagacaggc tggatttggg cagaaaggaa
      121 aagaatgcat gtttacctcc atacattctc tttatcgctc tcttccctac tttatgcttc
      181 aaattcttat acaaggttcg tagcagtaat gaaaccttgt gcatcttagc gcgcattaaa
      241 atgctccatg aagctaatca gaactctccc acgaatagga cttctggctc gagacaaaat
      301 ccagtagcgt tatgaacaac cttctgagcg tattctatca gttctttcat atcgcgagcg
      361 ctagcttctc cgatatttac gaatatgttt gcatgccatg gagagataag cgcattgcca
      421 actctatagt ttcgcaagcc aagttcatca agaatttttc cagttggttt accgaaatca
      481 tgattatttt tgaacataga ccctgcgctt ggaaagttga agtgtcgctt ttgcatgcga
      541 tctctcatgc gaaagcacat acgctcagcg attttcttag gatcatctga gatgagccta
      601 aacgatgcag cacagattat aaaggattct aattccccgc caggcatgaa gggtgagcgc
      661 ttataggacc atgactgagc actagcggga aggattttga atcttccttg gttgtcgata
      721 attgtaagat cttggatgtg cgtagatata tcatcttcgt aacagcgcgc attcatatag
      781 atcgaacctc caaggcttcc gggcattcca tagaaatttt caagcccaga aagaccacaa
      841 acctgagcct cttcgcacag agtaatcaaa ggaactccac attcggcata caacagaata
      901 gggtcgtttt tgtttttctg tttcaaatgc attatcgaag agaggagact tgtatcgatc
      961 accgcacccc taataccctt atcacctaca agtatattgg ccccgcctcc aagaatgaaa
     1021 aaaggtacat tctcaatctg tagaagctcc aatacctctt taaatgctaa tatagagcgt
     1081 ggccttataa agagatctgc gggtccaccg atcctgaaag tggtatgctc cttcataggc
     1141 tcgtcaaaag aaatcgagca atggtcagta ttgatttttt gagcgatttg ccatagcttt
     1201 ttcatgtaat tccagtatct tagcttaaag tagtaaaagc aagaaggtcc gactgacgaa
     1261 aaggagcgta tatgcatgat gtctacatat tcaatacgat gggtcggaac attcagcttt
     1321 ttaagcccct gcatgaaggg agtgtagggt tctatggctg cggacctaca gtctacaatt
     1381 atgctcatat aggaaatcta agaacatata ttttcgaaga tactcttgtc cgaatgctaa
     1441 agcgatttgg atatgaagtg actcatgtaa tgaatatcac tgatgtcggg catctctcta
     1501 gtgatgatga tacaggtgaa gacaagatgc ttaagtcagc cagagaaaga ggccagtcag
     1561 tgctcgaaat tgcgcaattc tatactgatg ctttttttag agacacagaa cgcctaaata
     1621 ttcaacagcc caaaatcatc tgtaaagcaa cagatcatat tcaggatatg atcgaactaa
     1681 tcaagcgtat cgaggcaaat ggttatactt atttcgctgg aggcaatctc tatttcgatg
     1741 tctccaaatt cgatcgctat ggtgagcttg ctgggcttca actagaccag ctcaaagcag
     1801 gagcgcgcgt cgagctagat cccaacaaac gcaatccttt cgactttgtc ctttggttca
     1861 caaaatcgaa attcgataag caagcgctac tctgggaaag tccatgggga ataggatatc
     1921 caggctggca tatcgaatgc tcagccatga gcatgaagta tttgggagaa caattcgata
     1981 tacatgcagg tggggtcgat catatcgctg tgcaccatac aaacgaaatc gctcaatccg
     2041 aagcagcgac gggccataaa tgggtcaatt attggatgca tgcggaattc ttagttatcg
     2101 acaagggcaa gatgtctaaa tcttcgggga gctttcttac gcttgagtct cttatcgata
     2161 gaggctacga tcccctggac tatagatatt tttgtctggg tgggcattat cgcagccagc
     2221 tctcattctc ctgggaagga ctggatcaag ctaaatctgc aaggctttcc ctggtggata
     2281 aggttcttgc ccttaaagaa aaggtagcga agccagcagc tctttcggag ctgggcgaaa
     2341 aagcgcttca atatcgtagc gccttcgacg aagctctagc cgatgacttg gcaatgccac
     2401 gcgcaatggc tcaggtgtgg aacttgctca aggataatga tgtacccgct caggatgctc
     2461 ttgcgctcat tttagatatg gatgaagtac tgggtctcga tcttgctaag gctcaaagaa
     2521 aagaagtctc ggtagatcca gagactcttc aatggatcaa tagtcagata gagagaagag
     2581 cagcggcaaa aaaggcgcga gattttgcga cagctgatgc aattcgcagc gagcttattg
     2641 ctaaaggcat tcagcttgaa gatagacctg agggaacagt gtggagattg cacgaatgat
     2701 tccaatgggc tttctgtaac aaaaggattg atagattgtt ccaaaataag gatgatgggc
     2761 caatatcaaa ctacaacctc atctttgcac agaatgtcga cctgcttcta cgcattgccg
     2821 tgcatattac aaatgattgg gaatctgctg aagacatagt tcaagatgcc tttagcaagt
     2881 tgatcgaaaa gcagatgcga ttcccttcag aagaggatgc ccgatattgg ctcattcgag
     2941 tagtaaaaaa tggggctata aactggtcca ggcgaaaagt gagagaattc aaggcctacg
     3001 aacactggtg gaacacagaa acaacccagg caaagatttc tgcagatgct ctcgattcga
     3061 gggacatgca aaatcaacat gcaggaacgg atcaatctgc agatcatgaa attttgatga
     3121 aagaatgcgc ccaagaagta cgagatgcct tgatgaagat tcccgaaaag ctgcgaatag
     3181 tactcatcct caaagaatat gaggggatga attataaaga gattgccaag gttctcggta
     3241 ttaccgaagg aaatgtcaaa gtgagagctt tccgagcaag agaaactctt ctttcgatat
     3301 tgaatgaggg aggatcttat gtgtcccgat gaaagtctgc tgaccgctta tgtcgacaat
     3361 gaagttccat caccttggaa ggagcggatc gagatgcatc tgggccaatg tgagcgatgc
     3421 gcaaaccgcg ttgctcaata tcgtgctcta aagctagcct taaaagctgc tgatgttttc
     3481 gatgctccac aacgggaaga agcagccaaa agaatacagg cttctttgag aagtattaca
     3541 gacgaatctt cacattcttt gcgtaccgaa gccatatttg aaagatatcc gctgttctcg
     3601 gctctaatgt ccaggcgcat atctgtaccg cttccggtcc tcgctgcatc ccttctgatt
     3661 acagtatttt ttgctggcct tgcttttggc atttttggag cgcatagaac tacaggccaa
     3721 gcgctcgcac tgagcacaaa gcttccagag gaatcggcat cgaacataga gagtattgtt
     3781 tccttgcttt cccagacaga tccaaaccag ttcgtaacaa tacacgcgcc agggaatata
     3841 tcctctaaac ctctggtcga ttcaacgcct gtgtatgtga tatacaatcc ctccgggcgt
     3901 aaaccaacaa tcatggcttt ccccgtacag ggagagataa aatgaagcgt tctgctctcg
     3961 cacttgcagc attttcaatt ctgaatattg cgtggctttt tgctcagacg ccttccatcc
     4021 caacagctcc ttcatcaatt gtagcgcccg attcatcttc ttcaaatatt cctattttcc
     4081 aatttcagat tcgaacagca ttcaccttaa cacctgggat ggtagcagca atccagtcag
     4141 cttcgcctgg atctctaggc tcaaaagcag gtcaaggcga atccatcgac aaagcgaatt
     4201 ctcccaattg ggaagataaa gtagttcgag aagtgagtct cgcctctccg ctcggtatca
     4261 agatcgagaa cgatagactc acggcaataa tagtattcat gcctattgaa cttgtaaaaa
     4321 aagatattac tatgcttgtc cagaatcaca tctatatgcg aagcccggat aactcgattc
     4381 agctgaatac ctcggttcat acagtgagaa ttccagttga ttcagtgttt tattactatc
     4441 ccttgggcgg agactctaaa cagggtgcac ccatggtgat cgagattctt gtgaattcaa
     4501 agtaaaccca tgaagcagcg taggataatt acgctttgca ttttaattct tttagttgtg
     4561 acaggaataa ttgtctatct aaggatacaa cctaggcttt tagcaatcga tcctgtaacc
     4621 cctatgcctg gtgatcttgt caatatagtg ggacacaatc tgggcaagaa tggaggggaa
     4681 ctccttttag ataatgttcc tctaccttca caagctgtaa ttctttggag ccctgttctg
     4741 ataagctttc ggatgcccag caattatgat tcggctactg tgcaagcaag aacaaagcta
     4801 ggagtatcga atccccttat gatagcgctt gcttcgaaag tccccgcgaa ggcggaaaat
     4861 aatatagtgc agcagcagcc cccaagcatt acgagcataa agccttcgac aaatgctcag
     4921 atagggaaat tgatcgagct tagaggcagt tacttcggca tgccagagga tgagtctgcg
     4981 gtgttttttt ctaaagtgcc cattatttct gcggttgaag cggtagattc ggacaatttc
     5041 ataagagtcg aatcgatgag tctttttata gataaatgga atagctctta tatatccgct
     5101 tatgtgccag atggtgttga aaatgggtat gtcttagtcc gaacaagaaa tggtctctcg
     5161 aacgcctttc ctatttctgt ttcgcgcaat cctggcagat tgtcaagagg agattccgta
     5221 acatatacac ttgaagatac cctcgttatc catgttgccg catctctgcc cgatggtcgg
     5281 gtatccttca tcctctctaa acctccaaca actcagtatc agtcgacaat aacaacgatc
     5341 gagacaggat ccgatcatct aagagccgaa acggcagtat ggcaggaatt tagatttggc
     5401 gcagcggatg caaggctggg aagaatagaa ttaaggaggc gattagtcat cgatgtttgc
     5461 gaaataaaag cagatattgc cataggttca ctacaaccac ttggagctaa gcctcctgct
     5521 tttttgagtt cttatcttgc tgcagatgct ctggttcctt ccgccaatcc agcgatagtc
     5581 tcggcagctc tttcgatcca aaaaaaggag aagaatcctt ttcgacagat cgtgctggct
     5641 gctcagtgga cattctcgaa tataaagcta aatgaggaaa aacagatttt aagggatgac
     5701 gctatcaatg cgcttaaaag caaagctggt ggtatgcgtt ctcttgtttt aatcaattgt
     5761 gctcttctca gagcattggg tattccagcg atcccggtag ctggttttct tgtgactgaa
     5821 gatatgcgcc taattcctca ttattggtca gaatattatc ttgccggaat aggatggatt
     5881 ccattcgacc cagcccttgc aatagggata actccttcgg gttttcgtcc aatctttacg
     5941 gatagatcca gctattatca aggaattgat aatagacata ttgcaatggc tagggggtat
     6001 cagcttctgc cctctgtccc agtagaagca cagagcaaaa caaagatttc ctggtccctt
     6061 ttcgaatatg atgaagcggc ctatggatta agctatactt cgacttggaa tcctccagtt
     6121 cttctttcga cagcaatcca atgattctta caaaaagatg aacacacgac aattcgtttt
     6181 ttgtagtata ctaatagatg ccatgataca ataatccgtg ggaggagata tgtcctatat
     6241 taaatctatg gacccagaac tctatgctgc gatggaggct gaggcagaac gccagcaaga
     6301 aaaaatcgag ctcatcgcaa gcgaaaacta tacctccaaa gccgttatgg aggctgtcgg
     6361 ttcggtactt acgaacaaat acgccgaggg ataccccggg aagcgctact atggcggctg
     6421 tgaatttgtc gatattgctg agaatctagc ccgtgacagg gcgaagaagc tttttg
//
LOCUS       DAOSOB010000080         6442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_12125_length_6442_cov_51.131361, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000080 DAOSOB010000000
VERSION     DAOSOB010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12125_length_6442_cov_51.131361"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1334)
                     /locus_tag="PLS88_07700"
     CDS             complement(237..1334)
                     /locus_tag="PLS88_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU07265.1"
                     /translation="MENAHVMLRNVSKKFGSFKALDNISLEIAQGSFTTLLGPSGCGK
                     TTLLRSIAGFYQIDEGEILIDGKVINDLPSHLRNAIMVFQDYALFPHMSIRENITYGL
                     RIKKMPQQEINERLERTIQYLDIGTLLDRMPGQISGGQQQRVALARALIMEPEVLLLD
                     EPLSNLDANLRNSIRAELRQLQKRLNITTIYVTHDQSEALAMSDSIAVLNTGKIEQIG
                     SPFDIYYHPANRFVASFVGTVNFIEGVIIDIDEQYIHVRSDRIDIDVEGNKFKPEMLG
                     KLEKSEKVLLVIRPESMKIIDMQNAQNSASNIFQGQIDHFIFEGSNIRYWIKAFENTI
                     IIDIFDPGEAGIKDGIISFWIDPSKIHLIPL"
     gene            complement(1396..3021)
                     /locus_tag="PLS88_07705"
     CDS             complement(1396..3021)
                     /locus_tag="PLS88_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU07266.1"
                     /translation="MRHKAFKQKSYEISLIIVIIAIVASLLIFVLYPIVEVFLFPKAS
                     DFSNILKNARYMKTLKNSMLMMLLSTLSSTFLGFIFAYTVTRTNVPGKRIFKFISLLP
                     LFSPPFMVAFSYIMMFGKNGLITAGLFHAKVNIFGWHGLWLSQTIAFFPVAFLVMEGV
                     LQSISPMLEYAGRNLGATGFTLFRTVTFPLARPGVAGAALLVAIQVLADFGNPIIISG
                     SFSVLATEAWMRVEGWADIAGAAILSILLLLPSLVIFILQRYWVGKRSYVTITGKISQ
                     VDIQKTSPIVKWSLFTICTIVSIFVLLVYIGLIIGAFVNGWGYDWSFTLRYFKEISSR
                     SNELFRSLSYSAIAGFSSALFSMIAAYLVSRKRFIMRKFVDFTCILPAALPGIFLGIG
                     YSICFTKPPIDLYGTAAIIILSMIFWNISSGYQTGIGALQQISPSLNEAASNLGATSM
                     RIFREIEIPLIKVPFFSAFIMSFIRAITTLSVIVFLSTSKNAVVTFSIMNLISDGFYG
                     KAAALTCSLLVITFSALGLARLLMGKQVQLFKV"
     gene            complement(3133..4152)
                     /locus_tag="PLS88_07710"
     CDS             complement(3133..4152)
                     /locus_tag="PLS88_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU07267.1"
                     /translation="MKAIRVLFLALVLVTSIYAKAPEETLTIYAALDEKTSKELAEAF
                     TQATGIPAEIALQIEEAGTIAARIKAESARARADVFIGGNSNFHTDLAEGNYLEKYSS
                     PVIKEAGIDPRFMDPDGYWTGWYLGALCLVYNTKMYEQKFKPMGLNPPTTWDDLLNPA
                     YKGLVIGSNPATTGGAYLMVCAQIFRLGSEKAGFDYIRKLHQNVAQYTKGANGCIPLI
                     AQGEAYVGFAWGHDALKQKEQGNLPITIVFPKDTGFEIGCASIIKGAPHTEAAKKFID
                     FLLSPTAEQINAKNGYRYPVRAGVTMPPGVPPFEDLKLAPWDLKAAAVNVDAWKKQWS
                     EITGK"
     gene            complement(4203..5225)
                     /locus_tag="PLS88_07715"
     CDS             complement(4203..5225)
                     /locus_tag="PLS88_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU07268.1"
                     /translation="MNQNNFESRVTIDHVASLCGFSKATVSRVINREGSVKPATEAKV
                     WEAIKKLGYMPNNAASALSGGKTRTVAVYLPDVKTEYYAALLTGLEDVAEKKFFNIII
                     KTKNNKKALYDLAINNKVDAFVLRNNGLQPFDHDVLVAIKRQGIPVVFIGKPPTESDS
                     HAILIDNVGGARLMAHHYVQHGFKNILFIAGPKDNIDSNDRLYGFKMGLNEMSADIQT
                     LDIAHGDFQRESGYQAAQETFENHKYDAVFAASDHMALGAMLYCRSKGIRVPEDVAIT
                     GFDDTFFSEFLDPPLTTVRQPMQDIGSIAMETVLNILEHNSSRFQKMILPTQLQIRKS
                     CGCTHT"
     gene            5326..6321
                     /locus_tag="PLS88_07720"
     CDS             5326..6321
                     /locus_tag="PLS88_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU07269.1"
                     /translation="MLKITFINVGYGDSILIEELNDSKPIFSMLIDGGVPYMGMYCDS
                     YDCSPGRIPPFKYLQSRRIQSLDVLFLSHLHIDHVGGMPEVMQTCKFGYIWSSCKLNE
                     LDYLDNLNRAHIDNRIAKEMRTSLNLLADMQRIANINGKEIEVVGKRLATVELTDKLQ
                     ANIFEVDPFLVSRTEKMVNNLLNGTDQKAEKILMELDEIENNSSVALQLKYGDVKILL
                     PADLPSSFWRRYNKEPYSLKADILKIPHHGQADSMTKRLAGAINPTHAIVSVSRDNPF
                     GAPVPEVLSMFDSKTNFWVTDNIEMPPYFRQTGPHRAVLFEIQEDGSICAMNEPA"
BASE COUNT         1889 a         1291 c         1337 g         1925 t
ORIGIN      
        1 caagaaacgc aagttcgaag ggcattacga cgatttggtc tgtatgggta tgtttttatg
       61 aagatagatc ctacaaatgg taagaatcat ttagcataat cattttcttt tttataggat
      121 tcgcggaaga gttgcttgac ttaatacctc aaagctcgcc cttttatatt tacgcagaga
      181 tagccgctca aaagaattca taccgtgtag cagaagagga gaatatacct cttaggctat
      241 aagggaatca aatggatttt tgaagggtct atccagaaag aaattatgcc atcttttatt
      301 ccagcttctc cagggtcgaa tatgtcgatt attattgtgt tttcaaaagc ttttatccaa
      361 taacgtatat tggaaccctc gaaaataaaa tgatcgattt gcccctgaaa gatattgcta
      421 gcactatttt gggcattttg catgtctatg atcttcatgc tttcaggtct tatgacaaga
      481 agcactttct ctgatttttc aagcttgcct agcatttcag gtttgaactt gttaccttca
      541 acatcaatat cgattctatc tgatcttaca tggatatatt gctcatcaat atcgatgatt
      601 acaccttcaa tgaaatttac ggtaccaaca aatgaagcaa caaatctatt ggcaggatga
      661 taataaatat caaagggaga tccaatctgt tctattttgc ctgtattcag tacagcaata
      721 gaatctgaca tagctagggc ttcgctttgg tcatgggtta catatattgt agttatattt
      781 agtcgctttt gtaattgcct taattcagct ctaattgaat tgcgtagatt tgcatcgaga
      841 ttcgaaagag gttcatcaag caaaagaact tctggctcca taataagagc acgagcgaga
      901 gccactcgtt gttgttgtcc gcctgaaatc tggccaggca ttctatcgag cagtgtgcca
      961 atatcgagat actgtatcgt tctttctaag cgctcgttga tttcttgttg gggcattttt
     1021 ttgatgcgaa gtccataagt gatattttct cgtatgctca tatgaggaaa cagggcatag
     1081 tcctgaaata ccatgatcgc attgcgcaaa tgtgaaggaa gatcattgat aactttgcca
     1141 tcgataagga tttctccttc atctatttga tagaatccag caattgacct caagagagtt
     1201 gttttgccac aaccactagg tccaagcagt gtggtaaagc taccttgggc tatctcgagc
     1261 gatatattat caagggcctt gaaactgccg aatttcttgc tgacattccg tagcatgaca
     1321 tgagcatttt ccatttagta aaactcctct ataagaactt tggcagcact catattcctg
     1381 tggtatagcg ttgatctata ctttaaagag ctgcacctgc ttgcccatca ggagccttgc
     1441 aagtccgaga gctgaaaatg taatgactag caatgagcag gtaagagcgg ctgcttttcc
     1501 atagaatcca tcgctgatga gattcatgat gctaaatgtt acaactgcgt tttttgatgt
     1561 tgatagaaat acaataacgc tcaaggtagt aatggctctg atgaatgaca taataaaggc
     1621 agaaaagaaa ggcactttga tgagtggaat ttctatttct ctgaatatac gcatgctagt
     1681 tgctccaaga ttgctggcag cttcattgag agagggggag atttgttgaa gggctccgat
     1741 accagtctga tagccactgg aaatattcca gaagatcatg ctcaggatga tgatggctgc
     1801 agtaccatag agatctatcg gcggctttgt aaagcatata gaataaccaa tacctaaaaa
     1861 gattcctggc agcgcagctg gcaaaataca ggtaaaatct acgaatttgc gcatgataaa
     1921 tcttttcctt gagacaaggt aagcagcaat catggagaaa agagcactgg agaatcctgc
     1981 tattgctgaa tagctgagac ttcggaatag ttcattggag cgagaggata tttctttgaa
     2041 atatctcaag gtaaaggacc aatcatatcc ccagccattg acgaatgcac cgatgattag
     2101 gccgatatag acaagcagaa cgaagattga aacaatagtg catattgtaa agagactcca
     2161 ttttacaatg gggctcgtct tctgaatatc cacttgagaa attttgccgg ttatcgtcac
     2221 ataagagcgc tttccaaccc agtatcgctg cagaataaat atcaccaaag aaggcaaaag
     2281 tagcaagatt gaaaggattg ctgccccagc tatatctgcc catccttcaa ccctcatcca
     2341 tgcttctgtt gcaagtaccg aaaaactgcc agaaatgata atcggattgc caaaatcagc
     2401 aaggacttga attgcaacca gaagagctgc cccagcaaca ccgggacgcg caagcggaaa
     2461 agttacagta cgaaataatg taaagcctgt tgcgccgaga tttcgtcctg catattcaag
     2521 cattggagaa atcgattgga gaactccttc cataacaaga aaggcaacgg ggaaaaatgc
     2581 tatagtctgc gatagccata gaccatgcca cccgaatatg tttactttgg catgaaaaag
     2641 tccagccgtt atgagaccat ttttcccaaa catcatgata tagctaaagg ctaccataaa
     2701 tggcggagag aaaagcggca ataggcttat gaacttgaag attctcttgc ctggcacatt
     2761 tgttcttgtc acggtataag cgaaaatgaa accaaggaac gtcgaggaaa gtgtcgagag
     2821 gagcatcatc agcatgctgt tcttaagtgt cttcatgtag cgagcattct ttagaatatt
     2881 tgagaagtcg ctggcttttg ggaacaagaa tacttctaca attgggtata atacaaatat
     2941 caaaagagag gcaacaatag caataattac tattattaat gatatttcat aggatttttg
     3001 cttgaaagcc ttatgcctca tagtagcctc ttaaataaaa gtaaagaaaa agctccggcc
     3061 ctattaaaag ggatcgcctt taatcatgaa gcagaaacag atcccggagc ttattgtgca
     3121 ctttgttatc ttttatttgc ccgtgatttc agaccattgt tttttccatg catcaacatt
     3181 tactgctgca gctttcaaat cccatggtgc aagcttcaga tcttcaaagg gagggactcc
     3241 tggaggcatt gttacgccgg ctcttactgg atacctatag ccattctttg catttatctg
     3301 ctcagccgta gggctcaata gaaaatcgat gaactttttt gctgcttcag tatgcggagc
     3361 gccttttatg attgaagcac atcctatttc gaatcctgta tctttgggga aaactattgt
     3421 tatcggcaga tttccctgct ctttctgctt gagggcatca tgcccccacg caaatccaac
     3481 ataggcctct ccttgagcaa tgagaggaat acagccgttt gctccttttg tatactgggc
     3541 aacattctga tggagctttc tgatataatc gaaccccgct ttttcacttc ccaatctgaa
     3601 aatttgcgcg cataccatta aatatgcacc tccagttgtt gctggattcg aaccaattac
     3661 aagaccctta taagctggat tgagcagatc atcccaagta gtaggaggat ttaagcccat
     3721 tggtttgaat ttctgttcgt acatttttgt attgtataca aggcacagag cgcctagata
     3781 ccagccagtc caatatccgt ctgggtccat gaatctcgga tcaatgccgg cttctttgat
     3841 gaccggagat gaatatttct caaggtagtt cccttcagct agatctgtat ggaagttgga
     3901 gtttccacca atgaatacat ctgcccttgc acgtgccgat tcagctttta ttcttgcagc
     3961 gatcgtacct gcttcttcga tttgcaacgc tatctctgca ggtataccag ttgcttgagt
     4021 gaatgcttct gcaagttctt tagaggtttt ttcatccagt gctgcataga tagttagcgt
     4081 ttcttcaggt gcctttgcgt atatgctggt tacaagcaca agcgcaagaa aaagaactct
     4141 gattgctttc atgttttgcc tccttatggc aatatcaaaa atccgcctaa agcagctatt
     4201 tttcaagtat gtgtgcaacc acatgatttt ctgatttgaa gctgcgtagg caaaatcatt
     4261 ttctgaaatc gcgaactatt gtgttcgagg atgttaagga cagtttccat ggcgattgaa
     4321 ccaatatcct gcattggttg tcggactgta gttaggggag gatcgagaaa ttcagagaaa
     4381 aaggtatcat caaaaccagt tatggctaca tcttcaggta ctctaatacc tttgcttcta
     4441 caatagagca ttgcgcctaa agccatatga tcgctagctg caaagaccgc atcatatttg
     4501 tgattttcaa aagtctcttg agctgcttga taaccagatt cacgctgaaa gtctccgtga
     4561 gctatatcca aggtttggat atctgctgac atttcattta ggcccatctt gaagccatat
     4621 agcctatcgt tagaatctat gttgtctttt gggcccgcga taaacaagat atttttgaag
     4681 ccatgctgaa cataatgatg agccatcaac ctggctcctc ccacattatc gattagaatc
     4741 gcatgcgaat cgctttctgt aggtggttta ccgataaaga caacgggaat tccttggcgt
     4801 ttgatagcta ccagtacatc atgatcaaag ggctgaaggc cattgtttct gagcacgaat
     4861 gcatcaactt tgttgtttat tgctagatca tataaggctt tcttattgtt ttttgttttt
     4921 atgataatat tgaaaaattt tttttccgcc acatcttcca gccctgtaag taaggcagca
     4981 taatattcgg tttttacatc gggcagatag acagctacag ttcttgtctt tccgccagag
     5041 agagcgcttg ctgcattatt tggcatataa ccaagctttt taattgcctc ccagactttt
     5101 gcttcagttg ctggcttcac agatccttct ctattgatta cgcgagagac agtagccttt
     5161 gaaaatccac aaagagaggc aacatggtct atagtgaccc tagattcgaa attattttga
     5221 ttcataatgt aaccgcttac tattttaatc aatgttgtat acttgtcaag cataattttt
     5281 tcaatctata ctataaaaat cataattggc tgaaagaagc agaaaatgct aaagataacc
     5341 tttataaatg ttggatatgg cgattctata ctcatcgagg aattgaatga ttctaaaccg
     5401 atattttcaa tgctcatcga tggaggcgtt ccgtatatgg ggatgtattg tgattcatat
     5461 gattgtagtc caggccgtat acctcctttt aaatatcttc aatcccgaag gattcaatct
     5521 cttgatgtat tgttcctgtc ccatttgcat attgatcatg tgggcggaat gccagaagtc
     5581 atgcagactt gcaagtttgg ctatatatgg tcgagttgca agctaaatga acttgattac
     5641 ctagacaatc tgaatagagc gcatatcgac aatcggattg caaaggaaat gagaacgtct
     5701 ttaaaccttc tcgcggatat gcagagaatt gctaatataa atggaaaaga aatagaggta
     5761 gtgggcaaaa gactggcgac tgtggagctt accgataaat tgcaggcgaa tatttttgag
     5821 gttgatcctt tcttggtaag tcgcacagaa aagatggtga acaatctact aaatggaaca
     5881 gaccaaaaag cagagaaaat tcttatggaa ttggatgaaa tcgaaaataa ttccagtgtt
     5941 gccctgcaac tcaaatatgg cgacgtaaaa atccttttgc ctgctgattt gcccagctcg
     6001 ttttggaggc gatataataa agaaccctac tctctaaaag ccgatattct aaaaattccc
     6061 catcatgggc aggcggacag catgactaaa aggcttgcag gggccatcaa tccaactcat
     6121 gctattgtaa gtgtttctcg agataatcct tttggagcgc ctgtaccaga agtgctttcg
     6181 atgtttgatt cgaaaactaa tttttgggta acggataaca ttgaaatgcc gccttatttt
     6241 cggcagactg ggccacacag ggcagtctta ttcgaaatac aagaggatgg cagcatttgt
     6301 gcaatgaatg agcccgcatg atataatgag tcaaaataaa ttataaacaa tgctaagatt
     6361 cggagccgac tatcggacaa ccctccttat ttcatctccg ccagcagctt ctttgcgttt
     6421 tcattaccca tcgaggctgc tt
//
LOCUS       DAOSOB010000081         6227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_12666_length_6227_cov_70.692482, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000081 DAOSOB010000000
VERSION     DAOSOB010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12666_length_6227_cov_70.692482"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /gene="mtnA"
                     /locus_tag="PLS88_07725"
     CDS             complement(<1..963)
                     /gene="mtnA"
                     /locus_tag="PLS88_07725"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOU07270.1"
                     /translation="MPQTLRLDDEKGELIILDQTMLPNEMKYLRISKTEEIWEAIKNL
                     RVRGAPAIGIAAAYGVYLAVRNGQPSSMEDALRIFMEARAYLATSRPTAVNLFWALDR
                     MQKRLEKEMSQSSVQRLCAALREEADAIRKEDAQVCKSIGENSLSLLKKGWGLLTHCN
                     AGVLATSEYGTALAPIYLGQEKGYGFRVFADETRPLLQGARLTAWELKQAGVDVTLIC
                     DNMAASVMKKGWVQAVLVGCDRVAANGDTANKIGTLGLAILAAHFGIPFYVHAPRSTI
                     DMDCETGLEIEIEERSGEEITTKWYEKPMAPKGVKVFNPAFDVTD"
     gene            997..1965
                     /gene="lipA"
                     /locus_tag="PLS88_07730"
     CDS             997..1965
                     /gene="lipA"
                     /locus_tag="PLS88_07730"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOU07271.1"
                     /translation="MKRITTLIIIEPYNLQSITIHNDIFCKSVYNISMKTSISSPKPS
                     WLKVQLPHGQNWRNVEEVLASHGLHTVCDEAHCPNKGECWGSGTATFMIMGDTCTRGC
                     RFCAVKAARFGQPLDPEEPRHLAEAVKSLSLSYAVITSVDRDDLPDRGASHFSACIHA
                     IRNLSPSTRVEVLIPDYIGQELREVLSAMPDILAHNIETVRRLQSIRDQRASYDKSLQ
                     TLSEAASTGIPIKSSLLLGLGETEKEVLEALQDLRSAGVSLIVMGQYLQPTPNEIPVE
                     EYISPEKFDKYADMAKALGFDSVLSAPFARTSFHAREAWSASKKET"
     gene            1968..2264
                     /locus_tag="PLS88_07735"
     CDS             1968..2264
                     /locus_tag="PLS88_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07272.1"
                     /translation="MKLELIGKPEGCKLLRMTLEVEPPLSLSSRIISLSIRGDFFAIP
                     EEAFDEIQEELCGATIGEFADCFDRLAQEKALEFEGISSKGLMELIQKELSHGS"
     gene            2254..3036
                     /locus_tag="PLS88_07740"
     CDS             2254..3036
                     /locus_tag="PLS88_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOU07273.1"
                     /translation="MAHNFRLLKTGFHSAVFNMGLDEAILDSVSQGLCLPTLRFYGWN
                     PPAVSLGYFQGLQEEIDIEACKAFGIDAVRRITGGGAVFHHHEATYSIVIPLGHPLAR
                     PSIIESYRILLAGIIEGLAELGIRSEFAPINDIVSAGKKISGNAQTRKKGCVLQHGTI
                     IIEVDVDLMFSILRVPQEKAKGKLIEDIKSRVTSIRSCLSLPDSVSFEELFSTTIAAL
                     EKGFARALDLSYVRSEPTDLELQNAERLAQEKFSNNQWTARR"
     gene            3129..4844
                     /locus_tag="PLS88_07745"
     CDS             3129..4844
                     /locus_tag="PLS88_07745"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOU07274.1"
                     /translation="MKKENKPHYVIGIDYGTDSCRAVIIDAANGYEISSASAAYPRWS
                     QGLYCDPKINQFRQHPLDYIESLEVVMQKIHEKNGQDVLPFVEGIAIDTTGSTPCLIS
                     DTLCPLAMLPEFQDNPDAMFILWKDHTSTEEAFEITELARKWSIDYTMYEGGVYSSEW
                     FWAKLLHIARTNPNIIKAGFSAIEHCDWMPALLTGTNNLEKIKRSRCAMGHKAMWHES
                     FGGYPGDDFFEALDKQLVRIKKTLGRDTYTSDTEFGKLSSEWARRLHLRPGIPVGVGA
                     YDAHMGAVGGGVEEGVLVKVMGTSTCDMIVGPKTSSANQESTEHLVKGICGQVDGSII
                     PGMIGYEAGQSAFGDVYAWFKNLLLWPLKEFKAESAESADKLGISTSKLDDLIESIDS
                     RLMLAMDKAASKIEPEASSVLALDWLNGRRTPYANQKLKGAIVGLSLGTTAPMIYRAL
                     IESTAYGSRAIVERFEQENIKIKKVRAIGGVARKSKLVVQIVADVLGRPIEIVASDQS
                     VALGAAMFASVVAGIYDSVPEAQLAMKPEIETLIQPDLRRTALYDTLYKNYLKLGAFI
                     ENEFA"
     gene            complement(4953..>6227)
                     /gene="larA"
                     /locus_tag="PLS88_07750"
     CDS             complement(4953..>6227)
                     /gene="larA"
                     /locus_tag="PLS88_07750"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOU07275.1"
                     /translation="KAVIMHFELKYGDGSLPLDFDPSRILSVVLPKTRRTLGNARNAV
                     ERALAQPEGTEPLLEMLIARKPKKLVIIVNDITRPTPYSILMPPLVEVIEKAGIPDEN
                     VTILIATGIHDLHTHEQNLLVYGEEICRRFKIVSHDASDSSMLSYKGKLPSGYDFWLN
                     RIVDDADFLITIGVVMPHYFAGFSGGRKSILPGVVGKQTVERNHARMVEIMDNLPPIR
                     ENPISLEMIHAARIAGVDFILNVVVDEGENVVEVVAGDLEKAWYKAVDVSQEMYMIPI
                     PGKADITISAASGFPRDINLYQAQKALDHADRATKSGGTIILIAECRNGYGEKVFEEF
                     MNAGYSPEEIMSHIKAHFVMGGHKAYGFAKVAAEKRVIMVTALSESIVASCFAQKASS
                     LSAAIEMAIAQQGPEAKFIIMPEGSIAAPVII"
BASE COUNT         1664 a         1520 c         1408 g         1635 t
ORIGIN      
        1 atccgttaca tcgaatgcag gattgaatac tttaacaccc tttggagcca ttggtttttc
       61 ataccacttt gtcgtgattt cttcgcccga ccgttcctct atttcgattt cgagtcctgt
      121 ttcgcaatcc atatctattg tcgatctcgg cgcatggaca tagaagggaa ttccaaaatg
      181 cgctgcaaga atggcaaggc ctaaagttcc tatcttattc gccgtatctc cattggccgc
      241 aacgcgatcg cagcctacaa gcactgcttg tacccaacct tttttcatga cactcgctgc
      301 catattgtcg cagattaaag taacatcaac ccctgcttgc tttagctccc atgcggtcaa
      361 gcgagctccc tgcagcaatg gccttgtctc gtcggcgaat actcgaaaac catagccttt
      421 ctcctgtcca agatagattg gagcgagtgc cgttccatat tctgaagttg caaggactcc
      481 ggcattacaa tgagtaagga gaccccatcc ttttttgagg agcgataaag agttttcgcc
      541 gatagatttg cacacttgtg cgtcttcctt acgaatagca tcggcctcct ctctcagcgc
      601 agcacaaagc ctctgcaccg aagattgcga catttctttt tctagccgtt tctgcattct
      661 atccagggcc cagaaaagat tgaccgccgt aggccttgaa gtagcgaggt aggctcgagc
      721 ttccatgaat attcgcagcg catcttccat gctcgaaggc tggccattcc ggacggccag
      781 atatacaccg tatgctgctg ctatgcctat cgctggagca cctcgaactc gaaggttttt
      841 tatcgcttcc cagatttcct ctgttttcga gatccgtaaa tacttcatct cgtttggtag
      901 catggtttga tcgagaatga tcaattcgcc tttttcatca tcgagtctca atgtctgcgg
      961 catttgcata tcgagatctc tccttttctt acatttttga agcgaataac aaccttaatc
     1021 ataatagaac catataatct gcagtcaatc acgatccaca atgacatttt ctgcaaatcc
     1081 gtctataata tttcaatgaa aacatcaatt tccagcccaa agccatcctg gctcaaggta
     1141 caacttccgc atggccaaaa ctggcgtaat gtagaagaag tgctagcaag tcatggtctt
     1201 catactgtat gcgacgaagc gcactgtcct aacaaaggag aatgttgggg ttccggtaca
     1261 gcgaccttca tgattatggg agacacttgt actcgcggct gtcgcttctg tgctgtaaag
     1321 gctgctagat ttggccagcc tctggatccc gaagaaccac gacacctggc ggaagcggta
     1381 aaatccctaa gcctttctta cgcggttata acttctgtgg atcgagatga tctacccgat
     1441 cggggtgcaa gtcatttttc ggcttgtatc cacgcaatcc gaaatctcag tccatctact
     1501 agagtagaag tcctaattcc agactatatc ggacaggagc ttcgagaagt attgtccgcc
     1561 atgccagaca ttcttgccca taacatagag accgttcgcc gcctgcagtc tatacgcgat
     1621 caacgagcat cctacgataa gagcttgcag acactgagcg aagctgcgtc tacgggaatc
     1681 cccataaaat cgagcctttt gctcggtctt ggagagactg aaaaagaggt gctcgaggct
     1741 ctccaagatc tcaggtccgc aggtgtatcc ctcatagtga tgggtcaata cctgcaacca
     1801 acgccaaatg aaatccccgt agaggaatac ataagcccgg aaaagttcga caaatatgca
     1861 gacatggcaa aggctctcgg gtttgactcg gtgctgtccg ctccatttgc cagaacgagc
     1921 tttcacgccc gcgaggcttg gagtgcctct aaaaaggaga cataaaaatg aagctcgaac
     1981 tgataggtaa gccagaagga tgcaagcttt tgcgcatgac actcgaagtg gagcctccgc
     2041 tctctttgtc ttcgcggatc atttcccttt ctatacgagg ggatttcttt gcaattccag
     2101 aagaagcctt cgatgagatc caagaggaac tatgcggagc tacaatcgga gaattcgctg
     2161 actgcttcga tagactcgcc caagagaaag ctctagaatt cgaaggtatt agcagcaaag
     2221 gacttatgga actcattcag aaggaactca gccatggctc ataattttag acttctcaag
     2281 acaggatttc attccgctgt cttcaacatg ggactcgacg aagcgattct cgattcggtg
     2341 tcgcaaggtc tttgccttcc aactctccgc ttctatggct ggaatcctcc ggccgtatcg
     2401 ctaggatatt ttcagggctt acaagaagaa atcgatatcg aagcatgcaa agctttcggt
     2461 atagatgcag ttcggcgtat aacgggagga ggcgccgtat ttcatcatca tgaagctaca
     2521 tatagcattg taattcccct aggacatcct ctcgctcgtc ccagcatcat cgaatcatat
     2581 agaatattgc tggctggcat tatcgagggt cttgcagaac taggcattcg atctgaattc
     2641 gctcctatca acgatatagt aagtgcaggg aaaaagatct ccggaaacgc ccaaactaga
     2701 aaaaagggtt gcgttcttca acatggcact atcattatcg aggtagatgt ggatcttatg
     2761 ttctcgattt tgagagtccc tcaggaaaag gccaaaggta agctcatcga agatataaaa
     2821 tcgcgcgtta ctagcatacg gagctgcctc tctttgccag attcggtgag cttcgaagag
     2881 cttttttcga ctactatagc agcgctcgaa aaaggatttg cacgagcgct cgaccttagc
     2941 tatgtgcgat ccgaacccac agatctcgaa cttcaaaatg ccgaaaggct tgcccaggaa
     3001 aaattttcta ataatcaatg gactgcacgg cgataggcca aatgcacatc gctcttttta
     3061 tttgaacccg cacccttgct ttggtctatt tttcggtata atagctaatc ttaagaacgg
     3121 agattgagat gaaaaaagaa aacaaacctc attatgtcat tggcatagat tatggaacag
     3181 attcgtgtcg agcggtgata atagatgctg cgaatggcta cgaaatatcc agcgcaagcg
     3241 ctgcataccc tagatggtca cagggacttt attgcgatcc aaaaataaac caattcaggc
     3301 aacatcctct ggattatatc gaatcacttg aagttgtcat gcaaaagata catgaaaaaa
     3361 acggacaaga cgtccttcct ttcgtggaag gcattgccat agatactaca ggttcgacgc
     3421 cttgtcttat aagcgatact ctctgccctc tcgccatgct ccctgagttt caagacaacc
     3481 cagacgctat gttcatattg tggaaggatc acacttctac tgaagaggct ttcgaaatca
     3541 ccgaacttgc ccggaaatgg agcatcgatt atacgatgta tgagggcggc gtatattctt
     3601 cggaatggtt ttgggcaaaa ctcctccata tcgcgcgcac aaaccccaac atcattaaag
     3661 ctggcttttc tgcgatagaa cactgcgact ggatgcccgc ccttctaact ggaaccaata
     3721 atctcgaaaa aataaagcga agccgctgtg caatgggtca taaggcgatg tggcatgaga
     3781 gcttcggggg ctatccaggc gacgatttct ttgaagctct cgataagcag cttgtgcgta
     3841 tcaaaaagac cttaggaaga gatacctaca caagcgatac tgaatttgga aagctctcga
     3901 gcgaatgggc tcgaaggctt catcttagac ctggtattcc agtgggggtc ggagcctacg
     3961 atgctcatat gggagcagtc ggaggcggag tcgaggaagg tgttcttgtg aaggttatgg
     4021 gcacaagcac ctgcgatatg attgttggac caaaaacatc ttcggcaaat caagaatcaa
     4081 ccgagcatct tgtgaaagga atatgtggac aggtcgacgg ttccatcatt cccggcatga
     4141 taggatacga agcgggccaa agcgcttttg gtgatgtcta tgcatggttc aagaatcttc
     4201 tattgtggcc actgaaagag tttaaagccg agtccgctga atcagcagat aaattaggca
     4261 tctccacaag caaacttgat gatttaatag aaagcataga ttctcggctc atgcttgcca
     4321 tggataaggc agcttcgaaa atcgaaccag aggcatccag tgttctcgct ctcgattggc
     4381 tcaatggaag gcgtactcct tatgccaatc agaaactcaa aggggcaatt gtcggcttaa
     4441 gcctaggcac tactgcacct atgatatatc gagcgctcat agaatctacg gcttatggtt
     4501 cacgagccat tgtagagcgt ttcgaacaag aaaacataaa gataaagaag gttcgggcca
     4561 taggcggagt tgcccgaaaa tccaaattgg tggtacagat tgtcgcggat gttcttggtc
     4621 gtccaataga aattgtcgca agcgatcaat ccgtagcctt gggagcggca atgtttgcgt
     4681 cggtggtagc aggaatctat gattctgttc cagaggctca attggccatg aagcccgaaa
     4741 tagaaacttt gatccagccc gatcttcgca gaaccgctct ctacgatact ctatataaga
     4801 attacctcaa acttggggca tttatagaaa atgagttcgc atgaagggct atagccaacg
     4861 cagaatacga acatatgcag attatacgcc cgtgcagatt acagaataag cgcctggttt
     4921 ttacttatga tttttaggca gttggccttt tcttatatga ttacaggcgc ggcaatactt
     4981 ccttccggca taattatgaa tttggcttct ggcccttgtt gggcaatggc catctcgatc
     5041 gctgcactga gcgaagaagc cttttgagca aagcagcttg ccactattga ctctgaaagg
     5101 gcggtcacca taatcactct cttctcggcc gccacttttg caaaaccata ggccttatgt
     5161 ccgcccataa caaagtgcgc tttgatgtgc gacataatct cttccggcga atagcctgca
     5221 ttcatgaatt cttcgaatac tttttctccg tagccattgc gacactccgc aataagtatg
     5281 atagtccctc cgctcttggt ggcacggtcg gcatgatcca gcgccttctg agcttgatat
     5341 agattgatat ccctcggaaa accagaagcg gccgaaatcg tgatatccgc ctttccagga
     5401 atagggatca tatacatctc ttggcttaca tcgactgcct tataccaagc cttctcgagg
     5461 tcgcccgcga ccacctcgac tacgttttcg ccctcgtcca ccacaacatt caggataaaa
     5521 tccacgcctg cgattcgggc tgcatggatc atttccaagc ttatcggatt ttcccgaatc
     5581 gggggcagat tatccatgat ttcgaccatc cgcgcatggt ttctttctac ggtctgcttt
     5641 ccgacaacgc ctggcaaaat ggactttctg ccaccggaga accctgcgaa ataatgcggc
     5701 atgacgacac cgatcgtgat aagaaaatcc gcatcatcca caattctatt cagccagaaa
     5761 tcatatcccg aaggtagctt ccctttgtac gagagcattg aagaatcgct tgcatcatgc
     5821 gaaacaatct tgaatctacg acatatctct tcgccataca caagaagatt ctgctcatga
     5881 gtatgaaggt catggatccc tgtggctatt agaatcgtca cattttcatc tggaatcccg
     5941 gctttttcta tcacttcgac aagaggaggc atcaagatcg aatatggtgt gggtctcgta
     6001 atatcgttga cgatgattac aagcttcttg ggctttcgag ctattaacat ctccaaaaga
     6061 ggttctgtgc cttctggttg cgccagcgct ctttctaccg cattgcgagc attgccaagc
     6121 gttcttcttg ttttggggag cactacactg agaatcctag atggatcgaa gtccaatgga
     6181 agagatccat ccccgtattt tagctcgaaa tgcataatga ccgcctt
//
LOCUS       DAOSOB010000082         5489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_14973_length_5489_cov_72.196724, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000082 DAOSOB010000000
VERSION     DAOSOB010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14973_length_5489_cov_72.196724"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..413
                     /gene="rpsH"
                     /locus_tag="PLS88_07755"
     CDS             15..413
                     /gene="rpsH"
                     /locus_tag="PLS88_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOU07276.1"
                     /translation="MSVSDPIADMLTKIRNANEARHEKVDIPTSKLKLEIVKILKTEG
                     YIKNFKKIQNEGQNFIRIFLKYDEKNDPIIHGLSKVSKPGRRVYSGYRDMPRILNGYG
                     IAIVSTSDGIITGRKAVEKQVGGELICTVW"
     gene            427..969
                     /gene="rplF"
                     /locus_tag="PLS88_07760"
     CDS             427..969
                     /gene="rplF"
                     /locus_tag="PLS88_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOU07277.1"
                     /translation="MSRIGMRPVAIPKGVKVTIEPTVFHVEGPKGKLSQEYCSDIKID
                     IKDGFAIVERKDDSKQSKAYHGLYRSLLDNMITGVSQGFKRSLVISGVGYRAEVQGNF
                     LVLSIGFTNDVIVVIPEGLKVSVDQNGQRIIIEGIDYAKIGKFASEVRSLRKPEPYKG
                     KGIRYEEERIRRKVGKTGVK"
     gene            980..1345
                     /gene="rplR"
                     /locus_tag="PLS88_07765"
     CDS             980..1345
                     /gene="rplR"
                     /locus_tag="PLS88_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOU07278.1"
                     /translation="MSIRELTDKRRKREKRRSHIRKDISGTPDMPRMTVYRSNLHLYV
                     QVIDDIKGNTLASASTMEKQFSNLRPTVNSGSVIGEEIGKRLMEKGITQVVFDRNGYK
                     YHGIVKAIADGARKAGVKF"
     gene            1355..1867
                     /gene="rpsE"
                     /locus_tag="PLS88_07770"
     CDS             1355..1867
                     /gene="rpsE"
                     /locus_tag="PLS88_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOU07279.1"
                     /translation="MERTRKEDSGVQEGYTEKLIQLNRTAKVVKGGRRFSFSALSVVG
                     DKQGHVGFGFGKANDVTEAIRKSVDRAKHAMVTVPVKKGTLPHEVTGVYKATKVYMKP
                     ACPGSGVIAGGPVRAILECAGYIDIISKCVGSRTAVNVVRAVFDGLDKLLDAQEVAKN
                     RGKSLKDMWS"
     gene            1870..2052
                     /gene="rpmD"
                     /locus_tag="PLS88_07775"
     CDS             1870..2052
                     /gene="rpmD"
                     /locus_tag="PLS88_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOU07280.1"
                     /translation="MPKIKITLVRSTIGQKPKARETVRCLGLRKIGSSTIQEASPQIL
                     GMVDKVKHLVEIKEVE"
     gene            2052..2525
                     /gene="rplO"
                     /locus_tag="PLS88_07780"
     CDS             2052..2525
                     /gene="rplO"
                     /locus_tag="PLS88_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOU07281.1"
                     /translation="MPDFYLHAPKGANKKKRIVGRGNSSGSGGTAGRGNKGQQSRSGG
                     KTYPGFEGGQMPLYRRLPGRGFSNHPFRKEYQIVNLCDLEAKFQNGDNVDTVSLFKKG
                     LVKKPELPVKVLGKGEIDIALTVSVDAVSSGAKAKIEAAGGKVETPTATGSSADA"
     gene            2543..3865
                     /gene="secY"
                     /locus_tag="PLS88_07785"
     CDS             2543..3865
                     /gene="secY"
                     /locus_tag="PLS88_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOU07282.1"
                     /translation="MANTLVDIFRVKELKDRILFTLLWLAIFRLGTYLPIPGINASAL
                     QSYFAQQSSTGSGGITDYLNFFAGGAFKNFSLFMMGVMPYISAQIIMQLLIVIFPKLK
                     TIAEEEGGRRKISRWTKIGTIVVAIIQSFSVTVWADRIPNAIVMSNRTLYTIVAILTV
                     TTGTMFLVWIGEQITKRGIGNGVSLLIFAGIVARLPNASYELIKSIQRGDINAVYAIV
                     VLVMFVAIVALVVVEQQGERKIPVNYAKRIVGRRMYGAQNTYIPFKINPSGVIPVIFA
                     SSLLTFPLQIAQSFGVQAKWLRDFSYWLRPSGFWYNLLYVIFIIFFAYFYTQVTLNPQ
                     EISKNIRENGGSIPGIRSDKMEEYLTRVLNRIILPGSLYLAIIALIPTLVQQMFNFPS
                     ELAYLLGGTSLLILVGVDLDTMAQVEAILKMHHYDGLVRKGRIKSRNL"
     gene            3918..4031
                     /gene="rpmJ"
                     /locus_tag="PLS88_07790"
     CDS             3918..4031
                     /gene="rpmJ"
                     /locus_tag="PLS88_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOU07283.1"
                     /translation="MKVRTSVKKICDKCKIIRRNGVVRIVCSNPKHKQKQG"
     gene            4048..4419
                     /gene="rpsM"
                     /locus_tag="PLS88_07795"
     CDS             4048..4419
                     /gene="rpsM"
                     /locus_tag="PLS88_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOU07284.1"
                     /translation="MARIAGVDLPNKHVDIALTYIYGIGRASAQKICETTGIDANKKI
                     NELSNEEIAELRKVIENDYKVEGRLRTEVALNIKRLMDIGCYRGLRHRRGLPVRGQRT
                     RTNARTRKGKRKTVANKKKAV"
     gene            4429..4827
                     /gene="rpsK"
                     /locus_tag="PLS88_07800"
     CDS             4429..4827
                     /gene="rpsK"
                     /locus_tag="PLS88_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOU07285.1"
                     /translation="MNVAATTTKTKKRKEKKSVYEGNVYIQATFNNTIVTVTDLKGNA
                     IAWSSSGSHEFRGAKKSTPFAAQTVTEAAIQKAMQAGLREAHVYVKGPGIGRESAIRQ
                     LGVMGLKVKSINDVTPIPHNGCRPRKARRI"
     gene            4844..5479
                     /gene="rpsD"
                     /locus_tag="PLS88_07805"
     CDS             4844..5479
                     /gene="rpsD"
                     /locus_tag="PLS88_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOU07286.1"
                     /translation="MARYIGPVCRLCRTENRKLFLKGDRCKSDKCPINKKRLPPGKAP
                     KTRMGKKSEYAVQLREKQALKRNYGLMERQFHNVFDEALRLPGKTGDNLIILLERRLD
                     NVVYRLHFASSRAQARQLVNHGHIAVNGKRVNIASSLVRPGDVISVIGQAKKMDSIRH
                     ALEEVQKSGVMPWLEVNADEMTGRYVINPQRQDVTDTLDIKEQLVVELYSK"
BASE COUNT         1590 a         1162 c         1463 g         1274 t
ORIGIN      
        1 ggtaggaggg tgcaatgagt gtatctgatc ccattgcgga catgctgacc aagatccgaa
       61 acgcaaacga ggctcggcat gagaaagtcg acatcccgac ttccaagctc aagcttgaga
      121 tcgtgaagat tctcaagacc gagggctata tcaagaattt caagaagatc cagaatgagg
      181 ggcagaattt tataaggatt tttctcaagt acgacgagaa aaacgacccc ataatccatg
      241 gattgagcaa ggtctccaag ccgggaaggc gcgtctattc cggatatcgc gatatgccac
      301 gcattctcaa tggctatggt attgccatcg tctcgacttc cgatggtatc attaccggta
      361 gaaaggccgt ggaaaagcag gtaggcggcg agcttatctg tacggtctgg taaggcgagg
      421 tagcgtatgt cgagaattgg aatgcgacct gtggcaattc ccaaaggcgt aaaggtgact
      481 atagagccga cggttttcca tgtcgaaggg ccgaaaggca agctttctca ggaatactgc
      541 tccgacataa agatagacat aaaagatgga tttgcaatag tggagcgcaa agatgattcc
      601 aagcagtcca aggcctatca tggtctttat cgcagcttgc tggacaacat gataactgga
      661 gtaagccaag gctttaagcg atctctagtg atatcgggcg tagggtatcg agcagaggta
      721 caaggcaact tccttgtgct cagtatcgga tttacgaacg atgtaattgt ggtgattccc
      781 gaaggcctca aagtctctgt cgatcagaat ggacagagaa taataatcga gggcatagat
      841 tatgccaaga ttggcaagtt tgcaagcgaa gtgcgatcgc tacgaaagcc cgagccctat
      901 aagggcaaag gaattcgcta tgaagaagag cgaattcgca gaaaagtcgg caagacaggc
      961 gtgaagtgag gcatatagga tgagcataag agaactaaca gataaaaggc gcaagcgcga
     1021 aaagcgcagg tcacatattc gaaaggacat ttcgggtact cccgatatgc ctagaatgac
     1081 agtgtatcgt tcgaatttac acctctatgt gcaggtaatc gacgatatta agggcaatac
     1141 ccttgcgagc gcctccacga tggaaaaaca atttagcaac ctcaggccta ctgtcaatag
     1201 cggcagcgtg attggagaag aaattggcaa aaggctcatg gaaaaaggaa ttacgcaggt
     1261 tgttttcgat agaaacggct ataaatacca cggtatcgta aaagcgattg ccgacggtgc
     1321 tagaaaagct ggcgtcaaat tctagggggc ggcagtggaa agaacaagga aagaggattc
     1381 cggtgtccag gaaggctaca ccgaaaagct tattcagctg aacagaactg ccaaggttgt
     1441 caagggagga cgaaggtttt ccttctcagc tttgtcggtg gttggcgaca agcaaggtca
     1501 cgttggattc ggctttggca aggcaaatga cgtcaccgaa gcaataagga aaagcgtgga
     1561 cagagcaaag catgccatgg taacggtacc ggtaaagaaa ggtacgctgc cccatgaggt
     1621 tactggcgtc tacaaggcga ccaaggtata tatgaagcca gcctgccccg gctctggtgt
     1681 cattgctgga ggccctgtgc gtgctattct cgagtgcgcc ggctacatcg atatcatctc
     1741 aaagtgtgtc ggttcgagaa cagctgtaaa tgtggtgcgt gcggtattcg atggccttga
     1801 taagctttta gatgcacaag aagtcgctaa aaaccgggga aagtcgctca aagacatgtg
     1861 gagttgatca tgcccaaaat aaagataaca ttggttcgca gcacaattgg tcaaaagcct
     1921 aaagcgcggg aaaccgtccg ctgtttgggg ctgcggaaga taggttcgag caccatacag
     1981 gaagctagcc ctcagatcct cggcatggtg gacaaagtga aacatctggt tgaaattaag
     2041 gaggtggagt gatgcctgat ttctatctcc atgccccaaa aggtgcaaac aaaaaaaagc
     2101 gaattgtagg cagaggcaac agttcgggtt caggtggcac agcaggccgc ggcaataagg
     2161 gtcaacagtc gcgctcaggc ggcaagacct acccaggttt tgaaggcggt cagatgcctc
     2221 tctacaggcg tttgcctggt cgaggattct caaaccatcc attccgcaag gaatatcaga
     2281 ttgtaaatct atgcgatcta gaagccaaat tccagaacgg agacaacgtg gatactgtga
     2341 gccttttcaa gaaagggcta gtgaaaaagc ctgagttgcc ggtaaaggtc ttgggcaagg
     2401 gcgagatcga tatcgctctc acggtgagcg tcgatgctgt ctcttccggt gcaaaggcaa
     2461 agatagaagc cgctggcggc aaagtggaga cacccactgc cacaggttct tcggcagatg
     2521 cgtaatataa aggcgggagc gaatggctaa tacactcgtc gacatcttca gggtcaagga
     2581 actaaaggat aggatactat ttactctgct gtggctagcg atttttcgcc tgggcacata
     2641 tctgcctatt cctggaatca atgcaagcgc gctgcagtca tatttcgcgc agcagtccag
     2701 caccggcagc ggcggtatta ccgattacct caatttcttc gctggcggcg catttaagaa
     2761 tttctcgctc tttatgatgg gcgtaatgcc ttatatcagc gcacagatta tcatgcagtt
     2821 attgatcgtt atcttcccta aactgaaaac aatcgccgag gaagaaggtg ggcgtcgcaa
     2881 gatttcgcgc tggacgaaga ttggaacgat cgtggttgcg attattcaga gtttttcggt
     2941 gactgtatgg gcagatcgca tacccaatgc aatcgttatg tccaatcgaa cgctatatac
     3001 gatagtcgcc attctcaccg ttacgacagg gactatgttc cttgtttgga taggtgaaca
     3061 gataaccaag cgcggaattg gcaatggagt atctctcttg attttcgccg gtattgtggc
     3121 gaggcttcca aatgctagct atgaactcat aaagagtatc caacggggag atataaacgc
     3181 cgtatatgca atcgtcgtgc tggtaatgtt cgttgcgatc gtagccctcg ttgtcgtcga
     3241 acagcaggga gagagaaaga tacctgtcaa ctatgccaag cgaatcgtag gacgtaggat
     3301 gtatggggct cagaatacct atattccttt caagatcaat ccttccggcg taattccagt
     3361 tatctttgct tcgagccttc taactttccc cttgcagatt gcacagagtt ttggtgttca
     3421 agcaaagtgg cttagagatt tctcgtattg gctcaggcct agtgggtttt ggtataattt
     3481 attatatgtg atttttataa tcttctttgc atatttctat acacaggtta cattgaaccc
     3541 tcaagagatc tccaagaata tccgcgagaa tggtggatcc atacctggca ttcgaagcga
     3601 caaaatggaa gaatacctta cgagggtctt gaatcgtata atacttcctg gatcgctcta
     3661 tcttgccata attgcgttga ttccaacgct ggtgcaacag atgttcaact tcccgagtga
     3721 gctggcttat ctgctgggcg gtacttcctt gctcattctt gtgggggtcg atctcgatac
     3781 catggctcaa gtggaggcta tcctcaagat gcaccactat gacggtcttg tgcgcaaagg
     3841 tcgcataaag tcgaggaacc tctagaaatt tttatgcgaa taggatttaa ttgcgtgttt
     3901 tggaataagg ggaacccatg aaggttagaa caagcgtcaa gaaaatttgt gataaatgta
     3961 agataatacg gcgcaatggg gtggttagga ttgtctgtag caatcctaaa cacaagcaga
     4021 aacaaggcta agagcaggag gaagctcatg gcaagaattg cgggagtcga cctccctaat
     4081 aaacatgtcg atattgctct tacatatatc tatggtattg ggagagcaag tgcccaaaag
     4141 atttgcgaaa cgactggcat agatgccaat aagaaaatca atgagctttc caatgaagaa
     4201 atagctgagc ttcgcaaagt catagagaac gactataagg tcgagggccg cctgaggacc
     4261 gaagtggcat tgaatatcaa gcgtctcatg gatattggct gctatcgtgg cttgcgccat
     4321 cgcagaggtc ttccggttcg gggtcagcgt acaagaacga acgcgcgtac ccgaaaaggt
     4381 aagaggaaga ccgttgcaaa caagaagaaa gctgtttaat agaggagcat gaacgtggct
     4441 gcgactacga cgaagacaaa gaaaagaaaa gagaaaaaga gcgtctacga aggtaatgtg
     4501 tatattcagg cgactttcaa taatacaatc gtaaccgtga cagacctcaa aggaaacgcc
     4561 attgcatggt cgagttctgg aagccatgaa ttccgtggcg caaagaaatc tacacctttc
     4621 gctgctcaga ctgtgactga agccgctata caaaaggcaa tgcaagccgg tctacgagaa
     4681 gcgcatgtct atgtgaaagg gccaggaatt ggtcgcgaat ccgcgattag acaactcggt
     4741 gttatggggt taaaggtaaa gtcgatcaac gatgtcacac cgattcctca taacggctgc
     4801 aggcctcgca aggctcgccg catctaggag ggggggtaca aaaatggctc gatatattgg
     4861 acctgtatgc agactgtgtc gcactgagaa cagaaagctt tttttgaaag gtgatcgttg
     4921 caaaagcgac aaatgtccga tcaacaagaa aaggcttcca cctggaaagg cccccaagac
     4981 tcgcatggga aagaagtccg aatatgcagt gcagctacgc gagaaacaag cattgaaacg
     5041 caattatggc cttatggagc gtcagttcca caacgtgttc gatgaagcct taaggctccc
     5101 tggcaagaca ggcgataatc ttattattct tcttgagagg cgcttggaca atgtcgtata
     5161 ccgattacac tttgcaagct cgcgcgccca ggctcgccaa ctggtcaatc atggccatat
     5221 agcagtcaat ggcaagcgag tcaatatcgc ttcaagtctc gtaaggccag gcgatgttat
     5281 ctcagttata gggcaagcaa agaagatgga ttcgatacgt catgccctgg aagaagttca
     5341 aaaatccggt gtaatgccat ggctcgaggt gaatgcggat gagatgactg gtcgctatgt
     5401 catcaatcca cagcgccaag atgtcacgga taccctggat atcaaggaac agctggtagt
     5461 cgagttgtac tcgaaataat aaggagcgg
//
LOCUS       DAOSOB010000083         5232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_15913_length_5232_cov_87.834846, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000083 DAOSOB010000000
VERSION     DAOSOB010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15913_length_5232_cov_87.834846"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="PLS88_07810"
     CDS             <1..1212
                     /locus_tag="PLS88_07810"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOU07287.1"
                     /translation="HENQAELTALLAAFCFFLSAIEYMLPKPLPFMRIGIANLPILLA
                     VDILPFKWFMVLAAAKVVGMSIVSGTLFSYVALFSLCGTMIAAIAMRATRKLGKGAIS
                     LIGVSISGAMASNAIQMLMARYVIFGEAAWLIAPIFLATGLATGTLMGLFAEFFAENS
                     HWYASARGIGNARSVNNQNNSEVGKRPTGSELAIEDSNSETEDNNSDTKAIDNSGKAA
                     ENKESLRKNIHNQGRIARRKRYEEYMEPWVAAVLGAAIAIFFLLQKGIVVKLLYLILF
                     IFWTWISGKRFSLISTFAVIAGIILANLLIPSGRVIFKIGPFVITEFALIDGFSKALT
                     FEGLMYLSKAAIMSGLRFPGRFGAIIAQAFQYYDHIIEYRGKVRAKTIVQDIDDLLQT
                     MSNGIDRKTQD"
     gene            complement(1271..>2656)
                     /locus_tag="PLS88_07815"
     CDS             complement(1271..>2656)
                     /locus_tag="PLS88_07815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOU07288.1"
                     /translation="ELMSVELDQVLFFTHLEYIATTFLPALLINFVLKFLDYDSPYNN
                     PILIALSIIAFAELILQWTTTSHGLVYRNVHLQAYGNLWVIHFEKGPVYILGAIIQEL
                     SIIGSMILFINALSKAKGTLRTQLSLIIIALSIPLVVFLLYLFSAIPVPYDINSFSFT
                     LVSIFTTILLLRVDMFAFAPVKQGRVFMSMRESCVIVDENGRIVDMNQRAKEMLPFLN
                     SDVIGEKALDVFAIFPSLIKAMIHPEEQEIELNASSVGGPEWILAKISLLNEDRKDSI
                     VYAIFFEDTTERHRMLESLQYYATRDNLTGLFNRRYFYEAIKPGIEVSITKGSEVSII
                     MIDVDSFKEVNDAYGHAIGDKVLRSCAKVLAAKLESESDAIIGRFGGDEFIVFLPEAS
                     LEEAQRIAEKLRLSVVAMPILEEIERPLHISVGVSAHQIARLEEIEMLISKADDALYK
                     AKRSGGNRVCI"
     assembly_gap    2657..2756
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2757..5142)
                     /gene="leuS"
                     /locus_tag="PLS88_07820"
     CDS             complement(<2757..5142)
                     /gene="leuS"
                     /locus_tag="PLS88_07820"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOU07289.1"
                     /translation="MAKYPFRDIEKKWQKRWEEEQTFRVFEDERFPKEKRRYVLDMFP
                     YPSGAGLHVGHPEGYTATDIYCRYLRMNGYNVLHPMGFDAFGLPAENYAIQTGTHPAI
                     TTYANIERFRSQIKALGFSYDWSREVITCDPDYYRWTQWIFLQLFKQGLAYEAEMPIN
                     FCPSCKTGLANEEVKDGHCDRCGTKVVRKRIRQWVLKITAYAERLLNDLDELDWPEPI
                     KLMQRNWIGKSEGASVNFAIEGFDEFLEVYTTRPDTLFGATYMVLAPEHPLVLKITKP
                     SQRRAVEAYMEEAASKSDLERTDLAKDKTGVFTGAYAINPVNGERIPIWISDYVLISY
                     GTGAIMAVPAHDSRDWDFAKKYSLPIVQVVAQPEEIAASESSDPASCDFTREPESYFD
                     GDGIAVNSGQFNGLTTVEFKERVVAWLEERGVAKKTVNYKLRDWLFSRQRYWGEPIPI
                     VHCKKCGIVALPEESLPLTLPEVKSYVPTGTGESPLAAITDWVNTTCPQCGGPATRET
                     NTMPQWAGSCWYFLRYLDPENSEIFAERSKIDYWMPVDLYVGGAEHAVLHLLYSRFWH
                     KVLYDLGLVNTDEPFMRLVNQGMILGEDNQKMSKSRGNVINPDDIIENYGADSMRMYE
                     MFMGPLEVSKPWSTAGLVGVSRFLERVWALSERPIVETPLNARLEKLLHKTIKKVTED
                     TASLNFNTAISQMMIYSNELAKLKSIPRAGWEPFVLLIAPYAPHLGEELWEKLGHKES
                     VSKSTWPVYREELTQDEEKEIVVQINGRVRSKFLAPAGTAQDKLIEMAKHAEKIS"
BASE COUNT         1338 a         1210 c         1181 g         1403 t
ORIGIN      
        1 catgaaaatc aagctgaact aacggccttg cttgctgcct tctgcttttt cttgtccgct
       61 atagaatata tgttaccaaa acccctgcct tttatgcgga ttggcatagc aaacttgcca
      121 attctgctag cggtagacat tctgcctttc aagtggttta tggtattggc cgccgcaaag
      181 gtggtaggca tgtcgatcgt gtctggaact ctcttttcgt atgttgcgct ttttagctta
      241 tgcggaacca tgatagctgc tattgcaatg cgagctacta gaaagcttgg caaaggagca
      301 atttcgctga taggagtctc tatttcagga gctatggctt cgaatgccat tcaaatgctg
      361 atggcacgat atgttatctt tggggaagcg gcctggctta ttgcgcctat ttttctagcg
      421 acagggcttg caactggcac tctaatgggt ttatttgctg aattctttgc cgagaattcg
      481 cattggtacg ccagcgcaag ggggattggc aatgcccgat ccgtcaataa tcagaacaat
      541 agcgaagtcg ggaaaaggcc cacaggctca gaattggcta tagaagatag caattcggaa
      601 acagaagata acaattcgga tacaaaagca atagataatt caggcaaggc agccgagaat
      661 aaagaatctc tgcgaaaaaa cattcacaat caagggcgca tagctcgccg gaagcgctat
      721 gaagaatata tggagccctg ggtagcagca gttctcggtg ccgcaattgc gatatttttt
      781 ttgctccaga aaggaattgt cgtaaagctg ctatatctca tcctgtttat cttctggaca
      841 tggattagtg ggaagcgctt ttctttgatt tcgacttttg cagtaattgc ggggatcatc
      901 ctagccaatc tgctcattcc atctggacgt gtgattttca aaattggccc cttcgtaata
      961 acggaatttg ctcttataga tggattctcc aaggcgctca cctttgaagg attaatgtac
     1021 ttatccaaag ctgccattat gtcaggatta cgtttccccg gaagatttgg cgctattatt
     1081 gcccaagcat ttcaatatta cgatcatatt atcgaatacc gcggcaaggt tcgcgcaaaa
     1141 acgatagttc aggacatcga cgatctgctt caaaccatga gcaacggtat tgatcgaaag
     1201 actcaggatt aggcattctg cctttggctt cactttggct tctatcatcg caagacaatt
     1261 ttatcaaacc ctagatgcat acccgattgc caccgctccg ttttgcctta tacaacgcat
     1321 cgtctgcctt gcttataagc atttctatct cttcaagacg cgctatttga tgagctgaaa
     1381 caccaacgct gatatggaga ggtcgttcaa tttcctcgag aataggcatt gctacaaccg
     1441 aaagcctaag cttttccgct attctctgag cctcttctag actcgcttcg ggcaggaaga
     1501 caataaattc atctcccccg aatcgaccta ttatcgcatc tgactctgac tctaacttag
     1561 ccgcaagaac cttagcacaa gagcgcaaaa ccttgtcgcc tatcgcatgg ccatacgcat
     1621 cgttgacctc tttgaatgaa tcgacgtcga tcatgataat gctgacttca gaacccttgg
     1681 ttatgctcac ttcgatgcca ggcttaattg cttcgtagaa atagcgacga ttgaatagcc
     1741 ccgtaagatt atcccgcgtc gcgtagtatt gaagactttc cagcatgcga tgccgttctg
     1801 tcgtatcctc gaaaaaaata gcatagacga tgctatcctt acgatcttca ttcagtagcg
     1861 atatctttgc aagaatccat tctggtccac caacagacga ggcattcaat tctatttcct
     1921 gttcttcagg atgtatcatg gccttgatta gagagggaaa tatcgcaaaa acatctagag
     1981 ccttctctcc tatgacatca gaatttaaaa atggcaacat ctcctttgct ctctggttca
     2041 tatcgacaat gcgaccgttt tcatcgacga tgacacagct ttctctcatg gacataaaaa
     2101 ctcgcccttg ttttacaggg gcgaacgcaa acatatcgac gcgcaaaagc aaaatagtcg
     2161 taaaaatgct cactaaggtg aatgaaaaag aattgatatc ataaggtaca ggaattgccg
     2221 agaagagata gagcaaaaat acaacaagag gaatactcag tgcaattatt atcaaggaaa
     2281 gctgagttct tagagttccc tttgctttgc tcaatgcgtt tataaataat atcatcgagc
     2341 caattatcga caattcctgg ataatagctc ccaatatata gacaggacct ttctcgaaat
     2401 gaatgaccca caggttacca taagcctgaa gatgaacatt ccgatatacg aggccatggc
     2461 ttgttgttgt ccactgcaga atcaattcgg cgaacgcaat tatgctcagt gcaatcaata
     2521 tgggattatt atatggagaa tcataatcga gaaatttcaa aacgaagttg atcaatagag
     2581 ctgggagaaa agttgtcgca atatattcaa gatgtgtaaa gaacagcact tgatcgagct
     2641 cgacactcat gagttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaga
     2761 aatcttctcg gcgtgctttg ccatttcgat gagtttatct tgagctgttc ccgcaggcgc
     2821 caggaacttg ctgcgtactc taccatttat ctggactaca atctctttct cctcatcctg
     2881 agttagttct tctctgtaga caggccaggt agacttcgat accgattcct tatggcctag
     2941 tttttcccag agttcctcgc caagatgagg cgcgtacgga gcgataagaa gcacaaatgg
     3001 ttcccatcca gcgcgtggga tagactttag cttcgctagt tcgttggaat atatcatcat
     3061 ctgtgagatt gcggtattga agttcaagct cgcggtgtct tctgttactt tcttgatggt
     3121 cttgtgtaga agtttttcga gccttgcatt caacggcgtc tctactatag ggcgctcaga
     3181 caaggcccaa acgcgttcta agaaacgtga gactcctaca agccccgctg tagaccaggg
     3241 tttggatact tctaatggac ccatgaacat ctcatacatg cgcatagaat cggcaccata
     3301 attttcgatg atatcgtctg gattgataac attgccgcga ctcttcgaca ttttctgatt
     3361 gtcttcgcct agaatcattc cttgattgac gaggcgcatg aaaggctcat ctgtgtttac
     3421 caagcccaga tcgtagagaa ccttgtgcca gaaccgactg tagagtagat gcaaaacagc
     3481 atgctctgct cctccgacat atagatcgac cggcatccaa tagtcgatct tggaacgctc
     3541 ggcaaagatc tcggaatttt ctgggtcgag atagcgaagg aaataccaac aggaacccgc
     3601 ccactgaggc atggtgttag tttcgcgagt ggcagggcct ccgcactgcg ggcaggtggt
     3661 atttacccaa tcggtaatgg ctgcgagggg cgattctcct gtaccggtag gaacatacga
     3721 tttaacctcc ggcagagtga gtggaaggct ttcttcaggc agagccacga taccacattt
     3781 cttgcagtgc acaatgggga tgggttctcc ccaatagcgc tgacggctaa atagccaatc
     3841 tcgaagcttg tagttcactg tttttttggc tacacctctc tcctcaagcc aggccacgac
     3901 acgctctttg aattccaccg tggttaggcc attgaactga cccgaattta ccgcaatgcc
     3961 atctccatcg aaataactct ccggctcgcg ggtaaagtca cacgaggcgg gatcggagct
     4021 ttcgctggcg gcgatctcct caggctgcgc aaccacctga acgatgggaa ggctatattt
     4081 cttggcaaag tcccagtcgc gcgaatcgtg ggcgggaacg gccattatgg cgccggtgcc
     4141 ataggaaata aggacataat cgctgatcca gatgggaatt ctttcgccat ttacgggatt
     4201 gatcgcgtac gctccggtaa agacacccgt cttgtccttg gcgagatccg tccgctctag
     4261 atcgctcttg ctggcagctt cttccatata tgcttctaca gctctacgct gactgggctt
     4321 cgtgatcttg agtacaaggg ggtgttctgg cgcaagcacc atataagtcg caccaaagag
     4381 ggtatctgga cgagtcgtat agacttcgag gaattcgtcg aatccctcga tcgcaaaatt
     4441 gacggatgct ccctccgact tgcctatcca gttgcgctgc atcagcttga taggttctgg
     4501 ccagtcgagc tcatcgagat cattaagaag gcgctccgcg taggctgtta tctttagcac
     4561 ccattggcgg atgcgtttgc ggacgacttt ggttccacag cggtcgcaat gaccatcctt
     4621 tacttcttcg ttggcgaggc ctgtcttaca agaaggacag aagttgatag gcatctcggc
     4681 ttcgtaggca agaccttgct tgaatagctg aaggaaaatc cattgagtcc atctatagta
     4741 atctggatcg caggttatga cctcgcgaga ccagtcatag gagaaaccaa gcgccttgat
     4801 ctgtgaacga aatctttcga tgtttgcata agtcgtgata gcaggatggg tacctgtctg
     4861 gatggcgtag ttttctgcag gcagaccaaa ggcatcgaag cccatgggat gcaagacatt
     4921 gtagccgttc attcgcaggt atcgacaata gatatcggtt gcggtgtaac cttctggatg
     4981 tcctacatga agcccggcac cagaaggata gggaaacata tctagcacat agcggcgctt
     5041 ttctttgggg aatctctcat cttcgaatac gcggaatgtc tgttcttcct cccagcgctt
     5101 ctgccatttt ttctcgatat ctctgaacgg atacttcgcc atgatttatc tcccgctatg
     5161 atgttcaaat aatgaaatat tgctatggat agtatctaag aggctttcgg atggcaagtg
     5221 ccgtaccagc aa
//
LOCUS       DAOSOB010000084         5193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_16091_length_5193_cov_75.759245, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000084 DAOSOB010000000
VERSION     DAOSOB010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16091_length_5193_cov_75.759245"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1580)
                     /locus_tag="PLS88_07825"
     CDS             complement(207..1580)
                     /locus_tag="PLS88_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOU07290.1"
                     /translation="MIVDIEEFGGLSDGYTDNSKAFAEAIAALREGGGGTLRMNGKAY
                     LTGPIELFSNISLEIGKNTTLSFVSDMNRYPPVLTRWEGVICYGMHPLIFAREAKNIE
                     LKGEGTIDGNGQPWWEALRRKKREGQNKPIEPFEMELAALNKYSGGQPSGGGGREMQF
                     LRPPLVQFFGCSDVTIEGLKFLNSPFWTIHPVFSDHISIRNISIQNPPDAPNTDGIDI
                     DSCKDISVSGSTIDVGDDCLAVKAGSGEQGIKEGKSSERIAIRDCCFKQGHGGVVIGS
                     ETAGGIYDVVVSGCSFEGTDRGVRIKTRRGRGGKVSRLRFSELHMKKVLCPVAINMYY
                     RCGAKPEEFPELFSLECRPVSASTPHISDIEISNLEAQECRASAGFIIGLPESPIEKL
                     RMQSCWIDLSKSEIVAPSESEMFEGLPETQARGIRIRHASCEFDKLEVFNLPKGEPPI
                     FYESPVD"
     gene            1747..>2140
                     /locus_tag="PLS88_07830"
     CDS             1747..>2140
                     /locus_tag="PLS88_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07291.1"
                     /translation="MQAHYPRIAFLLASAHTGAARGLWPELIAAAQSHRCILFVLPGG
                     RLNAIGGFEYMRNSVYRYAKASNIDSALCWASTLSGYAAESAVESFLIDTVDVPMVTF
                     GLKIDGQPFVGIDAYSGMKALVKHFIRSH"
     assembly_gap    2141..2240
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2259..>5193
                     /locus_tag="PLS88_07835"
     CDS             2259..>5193
                     /locus_tag="PLS88_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU07292.1"
                     /translation="MKALVKHFIRSHKRKHIAFIAGPRQHSSAEERYRAYCDALKEAH
                     ISYDSRLVCLDTPWSEGRRAMLDLIDGRGLVPGKDFDALCTASDLLAFEAGIVLRERG
                     IEIPSTISIGGFNDSEESRIFSPPLTTVRMPFTRQVLSALRLLIDMSKGNPSDDILLK
                     TKLVIRSSCGCVPQAFQMAGRTRVKARKSLPPSLIEAFSASLASQKNNSFFEHLEKIF
                     DEDRSSSSYETGMLENMLTALRLEFLSRASSRDEIDKIEKLIHQAMVALGLSKERKHE
                     YLLWKEKEDEQKLNLFNQELLCAKDIQSIIKAAAAHLPGLGILSGYLVTKDKQGNLMF
                     KGGYRKSKDTPYAEMIYPRPSREAIPRALFLPERIMPTEPGAYFILPLFYESTDLGHL
                     VIQAADIDPSLYEEIRSILSSALRGVKLFEEGDEARKRAERAEKSMSDIVSVISREIQ
                     SSIEAFDKISKLGAKGTQELEVSRLKELVRYLIDLSQAKSESVLTRAVLFDPYRFCAH
                     WAQNQLKTDKTNGKRTNRSESRLMCARPSSVYPLLFGDPKQLERMLTIVVQLLEETFH
                     IEELEISIVPSIQNCELRLDSVSKIPKEIAASGLNRIEKDIRFELAKQICLLHGGHIL
                     FSHNGGFLQVRIELPYPTVSGSIHNIPNREIEPCVIGPQGLSEHLPYGFLDKFSGKII
                     PYNSIFQESINPSQRVLLYLDSAEIDHESSAALSFILDKERFQNAECFIAQNSSSIME
                     TRLDNKPADVSGLLRSVLVSDSPSAIPILGSEASLQREIPIIRHVAQQPGIRLVACTS
                     PEAFEAASNRWRPVLFLVLEPTILKNQEFSTLLQRFPSVPLMAICRFFGAKEEWAAII
                     ERPHTVFCNTGEVFSEVLANLIQRSLEGKKFLPSPTGGIVADAIFYMNKSYSEQISRW
                     KMASYLNTSEDYLSRIFHTQMGISLWNYLARLRVQHAIGLLYSTGAGLAEIADLTGFQ
                     DEAYFC"
BASE COUNT         1317 a         1176 c         1167 g         1433 t
ORIGIN      
        1 tgatagaagc ctagccttaa aaaccagcga ccttttggag ctgcctcatg atggatacag
       61 gctggatttg ggcagaaagg aaaagaatgc atgtttacct ccatacattc tctttatcgc
      121 tctcttccct actttatgct tcaacttttt atacaaggtt cgtagcagta ccgaaagttt
      181 ctaggctttc ctatttctag gttttcctaa tcgactggag attcgtagaa gatgggaggc
      241 tctccctttg gaagattaaa aacttcgagc ttatcgaatt cgcagcttgc atgccttatt
      301 ctaattcctc gcgcttgggt ttccggtaag ccctcgaaca tctcggattc tgaaggagcc
      361 acaatctcgc ttttgctgag atctatccag cagctttgca tacgcagttt ctctattggc
      421 gattcgggca agccgatgat aaatccagcg gaagcgcggc attcttgggc ttccaggttg
      481 cttatctcga tgtcactgat atgaggagtg gaagcgctaa caggccggca ttccagcgaa
      541 aagagttccg gaaattcctc tggtttagca ccgcagcgat aatacatgtt tatagcgact
      601 ggacaaagga cttttttcat gtgtagctct gagaatctaa gcctgctgac cttgcctcct
      661 ctgcctcgcc tagtcttgat tctcactcct cgatctgttc cttcaaaaga acaaccgctt
      721 acaacgacat cgtatatgcc tcctgccgtt tcgctgccta taactactcc gccatgccct
      781 tgtttaaagc agcaatcgcg aattgctatg cgctccgaag atttgccttc tttaattccc
      841 tgttctcccg agcctgcctt gactgctagg caatcgtctc ccacgtcgat cgtcgaaccg
      901 ctaaccgaga tatccttgca tgagtctata tcgattccat cggtatttgg tgcatctgga
      961 gggttctgta tagagatgtt gcgtatgctt atatgatccg agaatacagg gtgaattgtc
     1021 caaaacggcg agtttaggaa tttgaggcct tcgatggtga catccgaaca cccaaaaaat
     1081 tggacgaggg gcggccgtaa gaactgcatt tctctgcctc cacctcctga aggctgacca
     1141 ccactgtatt tatttagagc tgcaagttcc atttcgaaag gctcgattgg cttattctgt
     1201 ccttctctct ttttgcgcct aagggcttcc caccagggct ggccatttcc atctatggtt
     1261 ccttcaccct tgagttctat gtttttggct tctcttgcaa atataagggg gtgcataccg
     1321 taacagataa ctccttccca gcgggttagg actggggggt aacgattcat gtcggaaacg
     1381 aagctcaatg tggtattttt gccaatttcc aggctgatgt tgctgaaaag ctcgatagga
     1441 ccagtgagat aggctttgcc attcatcctt agcgtaccac cgccgccctc tcttagggca
     1501 gcgatcgctt ctgcaaaggc ctttgaattg tctgtgtatc cgtcgcttag gccaccaaat
     1561 tcttcgatat ccactatcat cgagcttcct cttctattcc tcttctataa tgaagattta
     1621 tagaggagtt tcgagaaggc ttcaattgtg tttattggcc attaattgga cttttctgtc
     1681 tttttttaag taaataagca aagaatgttg acaattccaa gaagataagc ccacaattct
     1741 aaaagaatgc aggcacatta tcctagaatt gcttttcttc ttgcctcggc gcacactggc
     1801 gcagccagag gcctatggcc agaactcatt gccgccgctc aatctcatcg atgtattctc
     1861 ttcgtgctgc caggtggaag gctaaatgcc attggcggct ttgagtatat gaggaacagc
     1921 gtatatcgct atgctaaggc ttcgaatata gattctgcat tatgttgggc ttccaccttg
     1981 agcggatatg cagcagaatc ggctgtcgag agttttctca tagataccgt tgatgttcca
     2041 atggttactt ttggccttaa aatcgatggg cagcctttcg tcggtattga tgcatattcg
     2101 ggcatgaaag cccttgtcaa gcattttatc cgaagccaca nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn attgatgcat attcgggcat gaaagccctt gtcaagcatt
     2281 ttatccgaag ccacaagaga aaacatatcg ctttcatcgc aggtccgcgt cagcactctt
     2341 ctgcagaaga aagataccgt gcctattgcg atgccctaaa ggaagcccac atatcctatg
     2401 attccaggct tgtatgcctc gacactccat ggagcgaagg tcgccgtgca atgcttgatt
     2461 tgatcgatgg acgaggcctt gtgcctggca aggatttcga tgcgctatgc acggcgagcg
     2521 atcttctggc attcgaggct ggaattgtgc ttagagaacg aggaatcgag attccttcga
     2581 ctatttctat aggtggattc aacgactccg aagagagtcg cattttttcg cctccgctta
     2641 cgactgtgag aatgcccttt acccgtcagg tactttcagc gctgaggctt cttatcgata
     2701 tgagcaaagg gaatccttca gatgatattc tattgaagac aaagctagta atacgaagct
     2761 cctgcggatg cgttccacaa gctttccaga tggctggaag aacaagagtc aaggcgcgaa
     2821 aatcgcttcc gccaagcctt atcgaagcat tcagtgcttc tcttgcctct caaaagaaca
     2881 attctttttt tgagcatctg gagaaaattt ttgatgaaga tagatcgtcg tcttcctatg
     2941 agaccggcat gctggagaac atgctgacag cgctccgttt agaatttcta agccgagctt
     3001 cttctagaga cgagatagat aaaatagaaa aactaattca ccaggcaatg gttgcattag
     3061 ggctttccaa ggaaagaaaa catgaatacc tcctatggaa ggagaaagag gacgaacaga
     3121 aactcaatct cttcaatcag gagctgcttt gtgcaaaaga tatccagtcg ataataaaag
     3181 ctgctgctgc gcatctacca ggtttgggaa ttctctcggg ttatttggtg accaaagaca
     3241 agcagggcaa cttgatgttc aaaggcggct atagaaaaag caaggatact ccttatgcgg
     3301 aaatgatata tccaagaccc tcgcgggaag ccataccccg agctttattt ctgccagaga
     3361 gaatcatgcc aaccgaacca ggggcttatt ttattttgcc attgttctat gaatcgaccg
     3421 accttggcca cttggttatt caggccgccg atatcgatcc ttctctctat gaggagattc
     3481 gctcgattct atcttctgcg ctgagagggg taaagctgtt cgaagaaggc gatgaagcaa
     3541 gaaaacgggc cgaaagagcg gagaaatcaa tgtcggatat tgtatccgtt atatcgaggg
     3601 aaattcagag ctctatagaa gcttttgata aaatatcgaa gctgggtgct aaaggcacac
     3661 aggagcttga agtatctaga ctcaaagaac tcgtccgata tctgatcgat ctttctcagg
     3721 caaaatcgga atccgtgctt acccgcgcag tgctctttga tccttatcgc ttttgcgctc
     3781 attgggctca aaaccaattg aaaaccgata agacgaacgg caagagaaca aatagaagcg
     3841 aatcgaggtt aatgtgcgct cgaccttctt cagtctatcc cctcctattt ggcgatccaa
     3901 agcaactcga aagaatgctg actatagtgg ttcagttgtt ggaggaaacc tttcacatcg
     3961 aagaattgga aatctcgatt gtgccatcga tacagaactg cgaattgagg cttgactctg
     4021 tttccaagat tccgaaagaa atagctgcct ctggattgaa tcgtatcgaa aaggatatcc
     4081 gtttcgagct agcgaagcag atttgtctgc tccatggagg ccatattctc ttttctcata
     4141 atgggggttt ccttcaagta aggatagagc ttccttatcc aacggtgagc ggatccatcc
     4201 acaacattcc caatagagag atcgagcctt gtgtcatagg tccacaaggg ttgtcggagc
     4261 atttacctta tggatttcta gataaatttt ccggaaagat cattccctat aattctatat
     4321 ttcaagagtc aataaatcca tcgcagagag tattgctata cttggattct gcagagatcg
     4381 atcatgaaag ctcagccgct ctctctttca tactcgacaa ggaaaggttc cagaatgccg
     4441 agtgtttcat agcacagaac agttcctcca taatggagac tcgccttgat aataagcctg
     4501 cggatgtctc tggtttatta cgatcggtac ttgtgtctga ttctccttcg gctattccaa
     4561 ttcttgggag cgaagctagc ctacaaagag aaatacctat tattcgacat gttgctcaac
     4621 agccaggaat aaggcttgtt gcatgtactt ctccagaagc tttcgaggca gcgtcgaacc
     4681 gctggaggcc tgtccttttc ttggttctcg aaccgacgat attgaaaaat caagagttca
     4741 gtacgcttct gcagcgattt ccttcggtac cgctgatggc tatttgcaga ttttttgggg
     4801 caaaggagga atgggctgca attatcgaga gaccgcatac ggtcttttgc aacacgggcg
     4861 aagtgttctc cgaagtcttg gcaaatctga tccagagatc cctggaaggc aaaaaatttc
     4921 tccctagtcc gacgggtggc atagtcgcag atgcaatatt ctatatgaac aaatcgtata
     4981 gcgaacagat ttcgagatgg aaaatggctt cttatctcaa cacaagcgag gattatctaa
     5041 gcagaatatt ccatacgcag atggggatat cgctctggaa ctatcttgcc aggcttaggg
     5101 ttcagcatgc tatcggcctt ctttattcta ctggagccgg gcttgcagag attgcagatt
     5161 tgaccggttt ccaggacgaa gcatatttct gca
//
LOCUS       DAOSOB010000085         5054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_16707_length_5054_cov_70.509902, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000085 DAOSOB010000000
VERSION     DAOSOB010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16707_length_5054_cov_70.509902"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1269
                     /locus_tag="PLS88_07840"
     CDS             142..1269
                     /locus_tag="PLS88_07840"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU07293.1"
                     /translation="MKIIIIGNGVAANSAALAIREHERESDITMLSDENEFFYARPRL
                     TEYLAGKSPFEKIIIHDEKWYEKNNIDLLRGARVSSIDTEHRIVCGSFEDLPYDKLLI
                     ASGASPSFPPFYNPQLEKVFVLRTKEQADRIIAASQQCRSAAIIGGGLLGIETAYALS
                     ERGLESTIVEYYKWLLPRQLDEDSASILQELLLQKGIKFLLGKQTSSLSNENGKISIS
                     FSDGGSVYVDMTIISAGVRPNIAFLRDSKISIGRGITIDNQMRTNLPDVYAAGDCAEF
                     SGKMYGIWPAAKEQGEMAGHAIAGQSVDYRGSVMSTKLKVAGIDVASVGDFSISPSTT
                     VEVLHEEGVFKKYFREGNKLKGAILIGDTSDYFKLQKEIAK"
     gene            complement(1370..2293)
                     /gene="aroQ"
                     /locus_tag="PLS88_07845"
     CDS             complement(1370..2293)
                     /gene="aroQ"
                     /locus_tag="PLS88_07845"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOU07294.1"
                     /translation="MQSNNIAIIHGPNLNTLGQRETGIYGGKTLEEINDEIQLEASKF
                     PISLDFFQSNVEGFIIDFIHGCRGKIDGIVINAGAYTHYSIALRDSIAAVELPTVEVH
                     ISNVYKRESFRHLSVIAPVCVGQICGFSSYSYILGLHALLEILSGKNQQSSSIFPKKK
                     GKIKKFLSISGPNLNLLGQRKTSIYKEVSLDEIHSKLMKKAAELHVEVDCVQFNSEGD
                     IIDCMQTVPGKYEGVILNAGALAHYSYALRSAIGTMLVPCVEVFISNVYAREDFSHTS
                     VLSDVCVGVISGLGANSYVLALEALINLVKS"
     gene            complement(2460..3551)
                     /locus_tag="PLS88_07850"
     CDS             complement(2460..3551)
                     /locus_tag="PLS88_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOU07295.1"
                     /translation="MSQKIRINRGRLERFCTDVFIKLGMPQDEAQDSAEILVAADARG
                     IESHGVGRLWRYKNGLQKGIMKGGIQATTLRETPISLVLDANGAMGLSLSKRTMARTI
                     EKAIVSGAAFSSVRNSNHFGIAGFYSEMAARKDMIGIGMTNTAALGVPTFGRKAMFGT
                     NPIAFSIPAHDGRMFTLDMATTVVTRGKIEVYDRENKPLPSGWAVDTRGKGTSDAHRL
                     LEDMLYQRGGGLLPLGGEGELLSGYKGYGLAEVVDILTAILSGGVFGQAVIDSQATSA
                     RVCHFFGALRLDLFRDPEAIKFDMDRLLNELEKAEPAEGCQRVYYAGLKEHEAEIESA
                     RLGVQISEKVASQLRQIGEELGVELAGIF"
     gene            complement(3577..4497)
                     /locus_tag="PLS88_07855"
     CDS             complement(3577..4497)
                     /locus_tag="PLS88_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HOU07296.1"
                     /translation="MRDLKDYQNCALPETIEFIQGKGSLPLLHICNRYAECSVYLYGA
                     HIASFKPRDNKELLWLSPYSQFSEGTPIRGGIPICFPWFSKHRTMDNLPLHGFVRTRF
                     WNLESAEDFPDGRTKIVLNTESQEPLPDSWPYHFRLTITIIVGEKLEIALVIQNLENR
                     PIVCEDGFHTYFKVENPQKCQVKGLDSIDYIDRTKGDIRKKQNGPAYFEGEMVHAFMH
                     APQRLELVDLVDHRRICIEQENMDSTVLWNPGEYASAANPEILGTWNQFVCIESTNCM
                     DCQLNIPPLGSHYSILRLSSISFDEEENEE"
     gene            4571..>5054
                     /gene="dctP"
                     /locus_tag="PLS88_07860"
     CDS             4571..>5054
                     /gene="dctP"
                     /locus_tag="PLS88_07860"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOU07297.1"
                     /translation="MKKKSTVLVVFFLIAAIFLVSAQTEKKYVLKFGHVLTTQDPYHE
                     AFLKMAEAVSTRTNGNLKVDVYPSAQLGVEEDILEQIRQGANIGWNTDAARLGMYVPA
                     IAVINAPYFVDSLEEVKKLNTIPSVAAWKAELERTQGIKILSFMWVQGFRQMITNKPI
                     R"
BASE COUNT         1410 a         1189 c         1018 g         1437 t
ORIGIN      
        1 catgtttacc tccatacatt ctctttatcg ccctcttccc tactttatgc ttcaaattct
       61 tatacaaggt tcgtagcagt aataaagcac cttggctatt tcaatcaatt gctctatatt
      121 gataaaaaag gaacggatta aatgaaaata ataataatcg gcaatggggt agcggcgaat
      181 tctgcggcat tggcaattcg agaacacgaa agagaaagcg acattaccat gctttcagat
      241 gagaatgaat ttttctatgc gaggccgagg cttacagagt atcttgctgg aaaatcgcct
      301 ttcgaaaaga tcattatcca tgatgaaaaa tggtacgaaa agaacaatat cgatctattg
      361 agaggtgcta gagtcagttc tatcgatacc gagcatcgaa ttgtctgcgg ctcttttgaa
      421 gaccttccat acgacaagct gctgatcgcc tctggtgcat cgccttcgtt tcctcctttc
      481 tataatcctc aacttgaaaa agtgttcgtg ctacgaacca aagagcaggc tgatcgcatc
      541 atcgccgcct cacagcaatg cagaagcgca gcaatcatcg gaggaggtct tcttggaata
      601 gaaaccgcct atgctctttc agaacgcggc ttggaatcca caatcgtcga atattacaag
      661 tggcttctac caagacagct cgatgaagat tctgcaagta ttctccagga attacttctt
      721 caaaaaggca taaaattttt gctaggaaaa cagacatctt ccctttcgaa tgagaacgga
      781 aagattagca tatccttttc cgatggcgga tctgtttatg tcgatatgac gataatttcg
      841 gccggagtac gaccgaatat cgcttttcta agagattcca agatttcgat tggtcgcggt
      901 attaccatag ataatcagat gcgaaccaat cttccagatg tctatgcagc cggcgattgc
      961 gcagaattca gcgggaaaat gtatggaatc tggcctgcgg caaaagagca aggagaaatg
     1021 gctggtcacg ccatagccgg tcaaagcgtt gattatagag gaagcgttat gagcaccaag
     1081 ctcaaggtgg caggtatcga tgtcgcatct gtaggagatt tctcgatctc gccttcgaca
     1141 accgtggagg ttttacatga agaaggtgtt ttcaagaaat acttccgtga aggaaataag
     1201 ctaaaaggcg caattctaat tggcgataca tcagactatt tcaaattaca gaaagaaata
     1261 gcaaagtagt ctttatgcaa tatattgttc ttttaagcct aagaggcttc cccattcata
     1321 gcctctcctc actttacggc ttccgctaag gctcatatta gagctttctt caagacttaa
     1381 ccagatttat aagagcctct agcgccaaga catagctatt tgcacccaat ccagagataa
     1441 ctccgacaca tacatcggaa aggaccgacg tatggctaaa atcttctctc gcatagacat
     1501 ttgaaataaa aacctctaca cagggcacca acatcgttcc gattgcgctg cgcaaagcat
     1561 aactgtagtg tgcaagagct cccgcattga ggataacacc ctcatatttg ccaggcacgg
     1621 tttgcatgca gtctatgata tctccttcgc tattgaactg cacacaatcc acttcgacat
     1681 gcagttctgc ggcttttttc atcagcttcg aatgaatttc atccagcgaa acttctttat
     1741 aaatagaagt ctttctttgt cctagtagat tcagatttgg ccctgaaatc gatagaaatt
     1801 ttttaatctt tcctttcttc tttggaaaga ttgatgatga ttgttggttt tttcccgaaa
     1861 gaatctccaa caatgcatgg agacccaata tgtagctata agagctaaag ccacatatct
     1921 gccctacaca tactggtgca ataacggata aatgcctaaa agattccctt ttgtaaacat
     1981 tcgatatgtg tacttctacg gttgggagtt cgactgcggc tatagaatcg cgcaaagcaa
     2041 tcgaataatg agtataggcg ccagcattga taacaattcc atcgattttc cctctgcagc
     2101 catgaatgaa atcgataata aatccttcga cattcgattg gaaaaaatcg aggctgattg
     2161 gaaatttcga cgcctctaat tggatttcat cattgatttc ttcgagagtc ttgccgccat
     2221 atattccagt ttctctctgt ccaagtgtat tcaggttggg accatgaatt atcgcaatat
     2281 tattagattg catatgcttt cctgtctcaa gagcaattct acagcattgt acttgaatga
     2341 caagtatgta gacttacaat atggcgaaaa aaaacgttgc ctataattgg acattccact
     2401 tcatagcttt cgtcaaagag cttttctcta aaagcattgc ccagaatttg agctgtcatt
     2461 caaaaaatcc cagcaagctc cactcctagc tcttctccaa tctgcctaag ctgtgaagct
     2521 actttttcac tgatctgaac accaagacgg gcgctttcta tctctgcttc atgctcctta
     2581 agccccgcat agtagactcg ctggcagcct tcggcaggct ccgctttttc gagctcattg
     2641 agtaagcgat ccatatcaaa tttgatagcc tcgggatctc ggaagagatc gaggcgcaat
     2701 gccccaaaga aatgacaaac ccgcgccgag gtggcctgac tgtcaattac agcttggcca
     2761 aaaacaccgc cactcagtat cgcggtcagg atgtccacta cctctgctag cccataaccc
     2821 ttgtacccgc tcaacagctc accttctcct ccaaggggca gaagccctcc tcctcgctga
     2881 tagagcatat cttctagaag acgatgcgca tccgaagtgc cttttcctct cgtatctacg
     2941 gcccagccac ttggcaaagg cttattctct ctatcgtaga cttcgatttt tcctcttgta
     3001 acaactgtcg ttgccatgtc gagcgtgaac attcgcccat cgtgagcagg tatagaaaac
     3061 gcgatggggt ttgtgccaaa catagccttg cgaccaaatg taggtacacc gagcgctgct
     3121 gtattggtca tgccaatacc gatcatgtcc ttacgagcag ccatctcgct gtagaagcct
     3181 gcaattccaa agtgattgga attgcgcacc gaagaaaagg ctgctccaga gacaattgct
     3241 ttttcgatgg tcctagccat tgtcctctta cttagagaaa gacccatagc cccgtttgcg
     3301 tcgaggacga gcgaaatagg cgtctcgcgg agggttgtag cctgaatccc gcctttcata
     3361 atgccctttt gcaagccatt cttatatctc cataggcgcc ctactccgtg gctttcaata
     3421 ccgcgagcat cggcagcgac taaaatctcg gctgaatctt gagcctcatc ctgtggcatt
     3481 ccaagcttaa taaacacatc tgtacaaaac cgttcgagtc tgcctcgatt tatccttatc
     3541 ttctggctca ttcagcgcct cctctctcaa gctgctctat tcttcatttt cttcttcatc
     3601 gaatgagata gaactcaatc tcagtatact gtaatgcgat cctaatggag gaatgttcag
     3661 ctggcaatcc atgcagtttg tagactcgat acatacaaac tggttccatg tgcccagtat
     3721 ctctggattt gccgcacttg catattcacc tggattccag agcactgttg aatccatatt
     3781 ctcttgttca atgcatattc ttcgatgatc aacaagatcc acgagttcta atcgctgcgg
     3841 agcatgcatg aaagcatgga ccatttcgcc ttcaaaatag gctggaccat tctgcttttt
     3901 ccggatatct ccttttgtcc tgtctatata atctatgcta tccaatccct tgacctgaca
     3961 tttttgaggg ttttctacct taaaatatgt atgaaatcca tcttcacaaa cgatgggtcg
     4021 attttcgaga ttctggatga caagcgctat ttcaagtttt tcgccaacaa tgattgtaat
     4081 tgtcaatcta aaatgataag gccatgaatc cggtagcggc tcttggcttt ctgtattaag
     4141 cacaatcttt gttcggccat ccggaaaatc ctctgccgat tccaaattcc agaatcttgt
     4201 tctgacaaaa ccatggagag gaagattatc catcgtcctg tgcttactga accagggaaa
     4261 acaaatagga atccctccac ggattggcgt tccttcgcta aattggctat aagggctcag
     4321 ccataatagt tccttattat ctctaggttt gaagcttgca atatgggctc catacaaata
     4381 gacagaacat tccgcatatc tattacaaat atggagaaga ggtaaacttc cttttccttg
     4441 tataaactct atggtttctg gtaatgcaca gttttgataa tcctttagat ccctcatttt
     4501 ttatcttgac aacctcttga ataatgatat catctatctt agatattagc aaggttcaag
     4561 gaggcttgaa atgaaaaaga aatctacggt attagttgtc ttcttcctta tcgccgctat
     4621 tttcctcgtc tcagcccaga cggagaaaaa atatgttctt aaatttgggc atgtattgac
     4681 gacacaggat ccttaccacg aggcattctt gaagatggct gaagcagtat caacgaggac
     4741 gaatggcaat ctaaaggtcg atgtctatcc aagtgctcaa ctgggagtag aggaggatat
     4801 cctcgaacag atccgacagg gagcgaatat aggatggaat accgatgcag ccagactcgg
     4861 catgtatgtc cctgcaattg cagttatcaa tgctccatac tttgtcgaca gtctcgaaga
     4921 agtcaagaaa ctcaatacaa ttccttctgt agctgcatgg aaagccgaac tcgagcgtac
     4981 tcaaggaatt aagattctct cgttcatgtg ggtccagggt ttccgtcaga tgatcaccaa
     5041 taagccaatc cgta
//
LOCUS       DAOSOB010000086         4938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_17199_length_4938_cov_72.509523, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000086 DAOSOB010000000
VERSION     DAOSOB010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17199_length_4938_cov_72.509523"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1212
                     /locus_tag="PLS88_07865"
     CDS             133..1212
                     /locus_tag="PLS88_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOU07298.1"
                     /translation="MTTIVLSGGGGTRLWPLSRQALPKQYCDLLNGPTLFERTIKRNI
                     KFSDNLIVAINVSHFDIAKSQLEKVCPDKSARFILEPCGRNTAPAITLACLALDPDEL
                     VLVVPSDHIILDEEAYEDAVNRALVAASLGYLVTFGLKPEYAETGYGYIEVDSDEEAA
                     EKEFHHVRAFKEKPDRATAEAYIATGRYYWNSGMFAFLAGKYLNELGKTAAEILMASE
                     KAWKIARRSILPGGIGEVITIPQEEMFLIPAESIDYAVMEKSQNVAVVPCSFGWSDLG
                     SWDAIWNIREKSGIEAIEGPSIISVDSKRNLIIGSERLVALVDIEDLIVVDTPDALMI
                     SRRGSSQEVKKIVEILKEKGKTHLL"
     gene            1346..1966
                     /locus_tag="PLS88_07870"
     CDS             1346..1966
                     /locus_tag="PLS88_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU07299.1"
                     /translation="MSRERLFADKKDIINAAIELLSDKGYESFSTRSLAARLGVSPMT
                     LYNYFSDREEIIQAAIEAAYERIFEEFYNDLSSFFEKDSFCPLLGFIEMGKKLLLFSR
                     EQPKIYALVFIMNIMPYHEQPSVQRLFSITLSKVKDRLTAEEIGQDLQNHIYLFMLLV
                     LSLVQNVYNGNDSHDINAFEGNIQIAYDRLLKPFEKFFSFSNSTIR"
     gene            2052..2783
                     /locus_tag="PLS88_07875"
     CDS             2052..2783
                     /locus_tag="PLS88_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOU07300.1"
                     /translation="MSTAQKKKNFLIIISTLFGLVIWSFIAVTAGAQSAPSHSIQVNG
                     QALMYVEPDVANLSLGIEIQADSAANAQEQLLYQASMVLDALSGEGIQRSKMKTSFYR
                     ISPAYSTETGKENYIEGYKAETSISVEISDLESLAMIIDKAIAAGANVVRSVSYDRRD
                     LEAFKAQLIKSACLEASSKAEVGASALGLRLGAPIRVSIQDRFSALDTGNMVMKAAAF
                     GSESISPGEIEISVTVSAEFELIVE"
     gene            2870..3712
                     /locus_tag="PLS88_07880"
     CDS             2870..3712
                     /locus_tag="PLS88_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOU07301.1"
                     /translation="MNEKIGVIDSGMGGLTVVRSLFLRNVPADIVYIGDNANVPYGNR
                     PLDEIVELTLHMLEVLSERNIRIVAIACNTISATIDRLELFGEFSIVDIISPAARYLA
                     TCGESDVGLFGTEFTISSGLHAKLASDLNPAVRIHGVPSRRLAALIDESIEDESAIRE
                     EISSMLAQLVALHPVHKVLLGCTHYPIVMDLFKSLAPEMEFIDPADLQAKAVVELLKK
                     ADWQSNLIKNESIENAEQAEGVLFDELVRPSLEIITSGTEEAYRTMLNKLGIPEARRI
                     EHLI"
     gene            3727..>4938
                     /locus_tag="PLS88_07885"
     CDS             3727..>4938
                     /locus_tag="PLS88_07885"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOU07302.1"
                     /translation="MEIERIDPFSYLIPIREGMHVPARIYASESLFDLLCEDKSIEQL
                     VNVARQPGIVKQALGMPDMHQGFAFPIGGVAAFDINEGIVSPGGVGFDINCGVRVILT
                     SLDEEMVRPRLESIGKKLFAHVPSGVGSKGMLRVSAKEARKAMDNGLRWTLAKGFAED
                     SDLESVESGGYLEEADERAVSDRAVERGKEEFGTLGAGNHFLEIAKVERIFDTARAKA
                     YGLFEGQVLIWIHTGSRGLGHQVATEYIARMRPKMAQYKLPLYEHDFVSIPIRDPIAK
                     EYLSAMAAAANFAWINRQMITYAVREVFTEEFGEPWDSLGMQLLYDVAHNIAKFETHT
                     IEGMEKRLLVHRKGATRAFPAGHVDLSLHAREVGQPVLLPGDMMRGSYILVGGPRAME
                     ESFGSVAHGAGR"
BASE COUNT         1440 a          963 c         1170 g         1365 t
ORIGIN      
        1 attgccattc gatctcgtcc taaggatgat atttgccatt aaatccaaga agattattgc
       61 attctgaatg attcggcgct acagttcatt cagtcgatca tagaagcgaa aattagagga
      121 aaattgccaa gaatgacaac aatcgtcctt tcaggaggtg gtggtactag gctatggccg
      181 ctttcgagac aagctcttcc aaaacagtat tgcgaccttt taaacgggcc aacgcttttc
      241 gaacgcacaa ttaagcgaaa tatcaagttt tctgacaacc tcatagtagc aatcaatgtc
      301 tcgcatttcg atatagcaaa aagccagttg gaaaaagttt gtcctgataa aagcgctcgc
      361 tttatcctcg agccttgtgg tcgcaacaca gcgccagcga ttaccttagc ttgtctagcg
      421 ctcgatcctg atgagcttgt gcttgttgta ccttctgatc atattatact cgatgaagaa
      481 gcttatgaag atgcggttaa tagagctctt gtagccgcaa gccttggcta tcttgtaact
      541 tttgggctaa agccagaata cgctgagaca ggttatggct acatcgaagt agacagcgat
      601 gaagaggctg cagagaaaga gttccatcat gtacgcgctt ttaaagagaa acctgacaga
      661 gccactgctg aagcatacat tgcaacagga agatattatt ggaattcggg aatgtttgca
      721 tttctagctg gcaaatacct taatgagcta ggcaagacgg cagcagagat acttatggct
      781 tcagaaaaag cctggaagat agctcgaaga tctatcttgc caggaggcat tggagaagta
      841 ataaccattc ctcaagaaga aatgttttta attcctgcag aatcaataga ctatgcggtc
      901 atggaaaaaa gccagaatgt tgcagtagta ccttgttctt ttggttggag cgaccttgga
      961 agctgggatg ctatctggaa tatccgagag aaaagcggca tagaagctat agaagggcca
     1021 tcgattattt ctgttgattc aaagagaaat cttattatag gctctgagcg tttagttgcc
     1081 cttgttgata ttgaggacct cattgtggtc gatactcccg atgctcttat gatttcaaga
     1141 cggggttcga gtcaagaggt gaagaaaatt gtcgagatat taaaagagaa agggaagact
     1201 catctcttgt agaaaaattt ccgaaataga tactactata gataattatc taactattta
     1261 ttttgctaac aataaattct tacacaataa ataatctttt gtatattgac ttctcagaaa
     1321 gcttccttca aaataaggaa aatatatgag cagagaacgt ctttttgcgg ataagaaaga
     1381 tattattaac gctgcaatag aacttctaag cgacaaagga tatgagagtt tttctacacg
     1441 aagtcttgca gcgaggcttg gtgtatctcc catgacgctc tataattact tttctgatag
     1501 ggaagagatt atccaagctg cgattgaagc tgcctacgaa agaatattcg aagaattcta
     1561 taatgatctt tcatcatttt ttgaaaaaga ctctttctgt cctctcttag gctttataga
     1621 aatgggcaaa aaattgctat tgttttcacg tgaacagccc aagatatatg cgcttgtatt
     1681 cataatgaat atcatgccgt atcacgaaca gcccagtgtg cagagacttt ttagcattac
     1741 tttatcaaag gttaaggatc gtttaactgc agaagaaatc gggcaggatc ttcagaatca
     1801 tatttacctt tttatgctac ttgtcttgtc tctggtacag aatgtttaca atgggaatga
     1861 ctctcacgat ataaatgctt tcgaaggcaa tatccagatt gcatatgatc gacttctaaa
     1921 accttttgaa aaattttttt ctttctcaaa ttcgactatt cgatgatatt gattttttat
     1981 tgaacaagta actcaatgct tacctatatc taaggtttct atctcaacat ataataaaca
     2041 aggaggagat catgagcacg gctcaaaaga aaaaaaattt tcttattatc atttctaccc
     2101 tcttcggatt ggttatttgg tcttttatcg ctgttaccgc tggtgctcaa tcagcaccta
     2161 gccacagcat tcaggttaat ggtcaagcgc tgatgtacgt agagccggat gtagcaaatt
     2221 tgagtctcgg gattgagata caagctgata gcgctgcaaa tgctcaagag caacttctat
     2281 atcaagcctc catggttcta gatgctttat cgggggaagg catccaacga tctaagatga
     2341 agacaagttt ttaccgcatt tcgccggcat atagcacgga aacaggcaaa gaaaattaca
     2401 ttgaaggtta caaagctgag acaagcattt cagtggaaat ttctgatctg gaatctttgg
     2461 ccatgatcat cgacaaagca atagcagctg gagctaatgt tgtacgatca gtctcttatg
     2521 atcgtagaga tctagaagca tttaaggctc aactcatcaa atcagcttgc ttggaggcca
     2581 gctcaaaggc cgaggttggt gctagcgcgt tgggcttgag attgggagct cctataagag
     2641 tcagcataca ggacaggttc tcggcgctag atactgggaa tatggttatg aaggcagcgg
     2701 ccttcggctc agagtctata agtccaggag agattgaaat cagcgttaca gtgtctgctg
     2761 aattcgagct catagtggaa tagcaaagcc attgcaaaga aatgcgcttt tatcccagtc
     2821 ataagacatt cgattgacag aaagctatgg tcgtgtatca atattattta tgaatgagaa
     2881 aattggagtt atcgatagcg gcatgggcgg gctcacagtt gttcggagcc ttttcttaag
     2941 aaatgtacca gctgatatcg tctatattgg cgataatgct aatgtgcctt atggcaatag
     3001 accattggat gaaatagtag agctcacttt acacatgcta gaagtccttt cggaacgcaa
     3061 tattcgaatc gtggcgattg cgtgcaacac tatttctgct acgatagatc gcttggagct
     3121 ttttggcgaa ttttcgatag tcgatattat ctctccggca gcgcgatatc tcgctacctg
     3181 cggggaatcg gatgtaggcc tatttggcac ggagttcact atcagctcag gcttacatgc
     3241 aaaattggca agcgatctta atcctgccgt gcgcattcat ggagtaccaa gccgaaggct
     3301 agctgcgctt atcgatgaat caatagagga cgaatcagcc atcagagagg agatctcatc
     3361 gatgcttgcg cagcttgtag cgcttcatcc ggtacataag gtgcttcttg gctgcacgca
     3421 ttatcccatt gttatggact tattcaagtc tctggcacca gagatggaat ttatcgatcc
     3481 tgccgatctg caagccaaag ctgtagtcga gctgttaaag aaagcagact ggcaatccaa
     3541 tttgataaaa aatgaaagca ttgaaaatgc agaacaagcc gagggagtcc tttttgatga
     3601 attagtaaga ccttctctag aaattataac ttcggggaca gaggaagcgt atcgcactat
     3661 gctgaacaaa ttaggaattc cggaggctcg cagaatagaa catcttatct aagggcagga
     3721 aggcctatgg aaatcgaacg gatcgatccg ttctcatatc tgattcctat acgagaagga
     3781 atgcatgttc ctgctcgcat ctatgcctcc gaatcgcttt tcgaccttct ttgtgaagat
     3841 aaatccatag aacaattagt aaatgtagct cgacagcctg gcatagtaaa acaagcgctt
     3901 gggatgcctg atatgcatca gggttttgct tttccgatag gcggtgtggc tgccttcgac
     3961 ataaatgaag gcattgtatc gcccggcggt gtgggattcg acataaactg cggcgtaaga
     4021 gtaattctta cttctttaga tgaggaaatg gtccgtccga gactcgaatc tatcggcaag
     4081 aagcttttcg ctcatgtacc atcaggggta ggttcgaaag gcatgttgag ggtatctgct
     4141 aaagaagctc ggaaagcgat ggacaacgga ctgagatgga ctttggctaa gggatttgcg
     4201 gaagattccg acctcgaatc tgttgaaagc ggaggatatc tagaagaagc cgatgagcgc
     4261 gcagtaagcg atagggctgt agagagagga aaggaagaat tcggaactct gggagcaggc
     4321 aaccatttcc ttgagatagc taaggtggaa cgcatttttg atacagcacg cgccaaggcc
     4381 tatggcctat tcgaggggca ggtacttatt tggatccaca ctggatcaag gggtctgggt
     4441 catcaggttg ctacagaata catcgcgcgc atgcgcccga agatggcgca atacaagcta
     4501 ccgctgtatg aacatgattt tgtaagcata cctattcgcg atcctattgc caaggaatat
     4561 ctttcagcta tggcggcggc tgcaaacttt gcatggataa acaggcagat gataacttac
     4621 gctgtacgtg aggtttttac cgaggaattt ggggagcctt gggattcgct tggaatgcaa
     4681 ctattatatg atgttgccca taacatcgca aaatttgaga ctcataccat cgaaggaatg
     4741 gaaaagcggc ttcttgttca cagaaagggt gctacgcgtg catttcctgc tggccacgtg
     4801 gatctatctc tacatgcgcg agaagttggc caacccgttt tattgcctgg tgatatgatg
     4861 agaggcagct atatattggt tggcgggcct agagccatgg aagagagctt tggaagcgtt
     4921 gcacacgggg caggtcgg
//
LOCUS       DAOSOB010000087         4741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_18046_length_4741_cov_66.016859, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000087 DAOSOB010000000
VERSION     DAOSOB010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18046_length_4741_cov_66.016859"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..>2082)
                     /gene="glgP"
                     /locus_tag="PLS88_07890"
     CDS             complement(147..>2082)
                     /gene="glgP"
                     /locus_tag="PLS88_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOU07303.1"
                     /translation="IWRVDIGRAQLYLLDTNISENKPEDRIITATLYGGDMEMRIKQE
                     ILLGIGGIKALKALGIHVGATHMNEGHSAFLALERIRALMEEYGLDAHSAIQAFIPTN
                     IFTTHTPVPAGNERFGIDLMEKYFRYFITSLGLDWNEFLGMGRENPLDNQESFCMTVF
                     ALRLSARSNGVSKLHGEVSRRMWKGMWPELDLKEIPIGHVTNGVFPRTWISHDMLSLL
                     DKFLGPKFELEPDNHDVWAAIDQVSDEELWRTHERRRERLVAISRQRLAATYKRLSLP
                     DVEIVRSSDVLSPYALTLSFARRFATYKRGTLLLKDPDRLIRLLTDKNRPLQIVIAGK
                     AHPHDLAGKELIREIMHFSRREEVFGRLVFIEDYDMAMGRYMTSGSDVWLNTPRRPLE
                     ASGTSGMKAGMNGVLNCSVLDGWWAEAYSSEIGWAIGSGEEYKDEALQDEIEAEDLYD
                     LLEHEILPLFYTRGRDNIPRGWLKKMRSSMKTIGSEFATHRMLKEYYANYYEPALTES
                     RTLEVDNYRASISLAQYIEKVKRVWNGVRIVEFSDDGAPVIPRGSAIKIKALVELAGL
                     LPDDVAVECYLGRLSNKGEILEGVRMPMTLLEHEGQYYRYQCDIVSEITGQIGYSVRV
                     LPTHPALDGRFVPGLVRWAQ"
     assembly_gap    2083..2182
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2183..2842)
                     /locus_tag="PLS88_07895"
     CDS             complement(<2183..2842)
                     /locus_tag="PLS88_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HOU07304.1"
                     /translation="MKILSHTVKPALKGRLAKLDLIARNLWLSWNFDAVSLFTRIDRG
                     LWSASGQNPVRMLGMLSQEKIDELATDPAFLSDLDEVYSNFQRYLKNKIWYRGPRDRV
                     IAYFSMEYGLDVSLPTYSGGLGVLSGDHLKTVSDLGLPLVGVGILYRQGYFKQYLNAD
                     GYQLETYPENDWYNMPVERCVDAQGAPVLIQVDMAGRSISAAIWRVDIGRAQLYLLDT
                     NI"
     gene            complement(2885..4609)
                     /locus_tag="PLS88_07900"
     CDS             complement(2885..4609)
                     /locus_tag="PLS88_07900"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU07305.1"
                     /translation="MKRFLERSEYAEDRIIEFFALLVSDQFFVHKKDILVNDEGISYR
                     APLIASLIESYIEDSNASVNFKIPRSEEETVEKQARIRALVLTEHEEVLADLQKLFGI
                     AHFDSLVISSEEVLKEANTALSERAVVAMPVSLFLAATQEFGFKAREFGLVVLDGADL
                     FGDMPSEQQRRIWGYLLPPWQRRAVLFAEHIGIRTKNTALDFANDPETVVLKKAQASL
                     SQISTYMYRLSSQQKLKVLLALIQENSQEGRGAIIVFCNLRQTSKELELRLKLNHIKA
                     KHISAADPNSERRALFLEFAKIENEADKSQLVLIVSNDNLSVLPSKFASLAIHYDIPL
                     DPDIYLERVRIMRPKASTIIGLVCERYEVGLSAINSRFGLQFEFKEPTQNLLEYRDES
                     EGIPLRLEFEKRSSGHKAGHSAKQSKGPMKREGPNSPQKAEHKKPKEDFHQSKRASQS
                     SQKNLAKESPKRDQDSELYSMSTDERLAYFRKKYRSILDSPTEPKLSLKEEKTRSKPA
                     SASLSFEKIMPAEKANRGQTASVANSVEANKGDENETSGAKSDNPGIVKRFIERFFSG
                     SGSDERKD"
BASE COUNT         1194 a         1116 c          925 g         1406 t
ORIGIN      
        1 tttacatctg actacattaa aattctacgg gcagatttcc attttgtcaa cgcgataatt
       61 ttattttggc tacttttgcc acaaaattgt cgttcaccta tttcatttgt cattgctaat
      121 caatctgcta ttgaaactag aatctgctat tgagcccagc gaacaagtcc tggtacaaaa
      181 cgaccatcca aagctggatg cgtaggcaaa acgcgcacag aataacctat ttgacctgtt
      241 atctcgctca caatgtcgca ttggtagcga tagtattgtc cctcatgctc taatagagtc
      301 ataggcattc gcactccctc cagtatttct cccttgttag aaagcctacc taagtagcat
      361 tcgaccgcaa catcatctgg taaaagacca gcaagctcga caagagcttt tattttgata
      421 gcacttcctc ttggaatgac gggagctcca tcgtcgctaa attcgacaat gcgaacccca
      481 ttccaaaccc gtttgacttt ttctatatac tgagccagcg atatactggc ccgataatta
      541 tccacctcca atgttctact ctccgttaaa gcaggctcat agtaattggc atagtattcc
      601 ttgagcattc gatgcgttgc aaactcagag ccaatagtct tcatcgagga acgcattttc
      661 tttaaccaac ctcgtggaat gttatctcgt cctctggtat agaagagcgg taaaatttca
      721 tgctccagca ggtcatagag gtcctcagcc tctatttcat cctgaagggc ttcatccttg
      781 tattcctcgc cagaaccgat tgcccaaccg atttcggaac tataggcctc agcccaccag
      841 ccatcgagga ccgaacagtt gagtacacca ttcattccag ccttcatgcc tgaagttcca
      901 gacgcttcta gcgggcgccg cggcgtattg agccaaacat ctgagccact cgtcatgtag
      961 cgacccatag ccatatcata gtcttcgata aatacaaggc gaccaaaaac ctcttcgcgc
     1021 ctagaaaaat gcattatttc ccgaataagc tctttgccag ctaaatcgtg cggatgtgct
     1081 tttccagcga ttactatttg aagtggtcta ttcttgtcgg taagaagcct tattagccta
     1141 tctggatctt taagaagcaa ggttcctcgc ttataagtag caaatcggcg agcaaaacta
     1201 agtgtaagcg catatggaga tagcacatcg ctggatcgga cgatctctac atcgggcaag
     1261 ctaaggcgct tatatgttgc agcaagacgc tggcgactta ttgcaacaag gcgctctcgc
     1321 cgtcgttcat gagttctcca tagctcttcg tccgatactt gatctattgc agcccataca
     1381 tcgtgattgt caggctctag ctcgaatttc ggcccaagaa atttatcaag aagactgagc
     1441 atgtcatgac tgatccaggt tcgagggaag actccattgg ttacatggcc gataggtatc
     1501 tctttcagat ctaactcggg ccacatgcct ttccacattc tgcgcgatac ttcgccatgt
     1561 agtttggaca caccgttcga tcgagcggac aagcgcagtg caaaaacagt catacagaag
     1621 ctttcctgat tatctaatgg attctcgcgt cccatgccca agaattcgtt ccagtctagg
     1681 cctaatgaag ttataaaata acggaaatac ttctccatta gatcgatgcc aaagcgctca
     1741 ttgcctgcag gcactggcgt atgtgtagtg aatatattag taggtatgaa tgcttgaata
     1801 gctgaatgtg catctaagcc atattcttcc attagggctc ttattctctc cagagctaaa
     1861 aatgcggaat gtccctcgtt catgtgggtt gcacctacat ggatgcctag tgctttgagc
     1921 gccttgattc cccctattcc taacaaaatc tcctgcttta ttcgcatttc catgtcgccg
     1981 ccatagagcg tcgctgtgat gatacgatct tctggcttat tctcggaaat gttcgtatca
     2041 aggagataga gttgagctct tccgatatct acgcgccaga ttnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnaatgttcg tatcaaggag atagagttga gctcttccga
     2221 tatctacgcg ccagattgcg gcgcttatgg agcgacctgc catatcgact tgaattagca
     2281 caggtgcgcc ttgggcatcc acacagcgtt ccactggcat gttataccaa tcattctcgg
     2341 gataggtttc cagttgatag ccatcggcgt tcagatactg cttgaaatag ccctgacgat
     2401 aaagaatccc aacacccact agcggtaatc caaggtcgct cacggttttt agatgatcgc
     2461 ccgatagtac accgagccca ccagaatagg ttggtagaga aacatcgagc ccatattcca
     2521 tagaaaaata agcgattact ctatcgcgtg gtccgcggta ccaaatttta ttctttaggt
     2581 agcgctgaaa atttgaataa acttcatcaa ggtcagagag aaaagcgggg tcggtggcaa
     2641 gctcatcgat cttttcctgg ctcaacatac ccagcattct tacgggattt tgccctgaag
     2701 ccgaccacaa tcctcgatcg atgcgggtaa agagagacac cgcatcgaaa ttccacgaaa
     2761 gccataggtt tcgtgcaatt aaatcgagct tggccaagcg accttttagc gctggtttaa
     2821 ctgtatgtga caatattttc atttatcaat acctcttttg catttctgtg cctttatcta
     2881 ttctttaatc ttttctttca tccgatcctg atcctgagaa aaatctttct atgaaccgtt
     2941 ttactatacc tggattatcc gattttgcac cactagtctc attctcatct cccttattag
     3001 cctcaacaga gttcgctaca ctggccgttt gacctctatt tgctttctct gcaggcatta
     3061 tcttttcaaa ggacaatgat gccgatgccg gcttagatct tgttttttct tccttgagag
     3121 acaattttgg ttctgtcggt gaatctagga tgctgcggta tttttttcgg aaataggcca
     3181 ggcgttcgtc ggtactcata gaatagagtt cgctatcctg gtcccttttt gggctttcct
     3241 tagctaagtt tttttggctg ctctgcgaag ctcttttgct ttgatgaaaa tcttcctttg
     3301 gttttttgtg ctcagccttt tgaggactat ttggaccttc tcttttcatt gggcctttac
     3361 tttgcttagc gctgtgcccc gctttatgtc ctgaagaccg tttttcaaat tcaagcctta
     3421 aaggtatgcc ttcgctctcg tcgcggtatt ccaacaagtt ctgggtgggc tctttgaatt
     3481 cgaattgcaa accaaatctt gaatttattg ctgataagcc aacttcatag cgctcacaaa
     3541 ctagacctat tatggtagag gcctttggtc gcattattcg tactcgctca agatatatat
     3601 caggatctaa tgggatatca tagtgaattg ctagacttgc aaatttcgaa ggtagcacac
     3661 tcaggttatc attggaaacg attaaaacta attggctttt atctgcttca ttttcgatct
     3721 tggcaaattc caaaaaaaga gctcttctct ctgaattcgg atcagctgcc gatatatgtt
     3781 tcgccttaat gtgattaagc ttgagcctga gctctaattc cttcgatgtc tgcctcagat
     3841 tgcagaatac gatgatggct cctctgcctt cctgggaatt ctcttgaatc aatgcaagga
     3901 gtacctttaa tttctgctgt gaactaagac gatacatgta agtgcttatt tggcttaagc
     3961 ttgcctgagc ctttttcaaa acaaccgttt caggatcgtt tgcgaaatct agcgccgtat
     4021 tctttgtcct tatgccaata tgctcggcaa aaaggactgc cctccgctgc catggaggaa
     4081 gcagatatcc ccatattcgt cgctgctgtt cgctaggcat atctccaaag aggtctgctc
     4141 catcaagcac gacaaggcca aattctcgcg ctttgaaccc gaattcttgt gtcgccgcga
     4201 ggaacagaga cacgggcata gctactaccg ctctttcgga aagggccgta ttcgcttctt
     4261 tcaaaacttc ctctgacgat attactaagc tatcgaaatg agcaattcca aacagctttt
     4321 gtagatccgc caacacctct tcatgctcag ttagcactaa tgcacgtatt ctggcttgtt
     4381 tctctactgt ttcttcttcg cttctgggta ttttaaaatt cacagaggca ttagaatcct
     4441 cgatgtaact ctcaataaga gaagcaatga gtggcgccct atagctaatg ccttcgtcat
     4501 ttacaaggat atccttttta tgaacgaaaa attgatcgct gacgagcagt gcaaaaaatt
     4561 caataattcg atcctctgcg tattcactac gctccaagaa tcttttcatt tctaaccctt
     4621 ctctatattg caatatctat atatattgta ctgttgcttt tttaagcata atcacaaaaa
     4681 tgaaatacat atcataatag ataatcgaca ttaggtatat tgtggtaaat caataatact
     4741 g
//
LOCUS       DAOSOB010000088         4716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_18183_length_4716_cov_73.131517, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000088 DAOSOB010000000
VERSION     DAOSOB010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18183_length_4716_cov_73.131517"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /gene="glyA"
                     /locus_tag="PLS88_07905"
     CDS             <1..1092
                     /gene="glyA"
                     /locus_tag="PLS88_07905"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOU07306.1"
                     /translation="EFVDIAENLARDRAKKLFGADHANVQPHSGSQANMAVLFAALNP
                     GDTIMGMSLAHGGHLSHGAPVSFSGKFYKVVSYGVNPDTELIDFDQVAFLARQHKPKL
                     IIAGASAYSRIIDFDAFRKIADEVGANLMVDMAHIAGIVAAGRHPSPIATAHFVTTTT
                     HKTLRGPRGGMILVGRDMENDRGIMNAKGDALRRWSEVIDSMVMPGIQGGPLMHVIAG
                     KAVAFKEAQKPEFVEYIDHVLYNAKVMAQALTLGGARIVSGGTDNHLMLVDLTPLGIN
                     GRDAEKWLDEANITVNKNSIPFDKKSAFVTSGIRIGTPAITTRGMGPSEMEQIASFIM
                     EVLKSKGDAQVIARIKQEVLALTSRFPLL"
     gene            1109..2326
                     /locus_tag="PLS88_07910"
     CDS             1109..2326
                     /locus_tag="PLS88_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOU07307.1"
                     /translation="MERPELDEEANRRAAYWLLLNAKEQGFFSYGLLSQTAWEQVVES
                     QGAIEVESSEAKQVNRDSLRSGVAETEQLQAESSAVKVLKEHYHLLDARAMLVVALRY
                     APDIEAEAAAGRAERGLPPLPLACYGQPAAKVARFARGNWYREIMDRLGHCAVATFEA
                     ITRERLAESLDLEGKPPSRWPRFVNSRFPEKALALAAGLGTIGRNSLLIAKKTNDSDI
                     LNLLDFQEHLIKSDSHNACIMNLPIWSSAVVIGLMLLPFDVKMEKLEDKDSPRGIAPS
                     FFAVCSDCRLCIDACPSRALSISSIPGFERYLCIQNYSSIEGNLPDFINNSWTDQLYG
                     CDLCLEACPYFEPDEEAHVNHGRIGGFFDAGSLANLNDEQIRVLFKGSALDQKWISPK
                     ALRRNAAIIAKKA"
     gene            2411..2782
                     /locus_tag="PLS88_07915"
     CDS             2411..2782
                     /locus_tag="PLS88_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07308.1"
                     /translation="MKKFIAVFAIISIAFFACTPKKADKDNQTSKNISSIDPEEIKDI
                     TTWKAAYDDLIGAYSSAVSQLNGGNLGTGAKIEELGKRAEALNSVAETIKSGLSVQEQ
                     ADFAAVIEEYKEKFNQAVNSN"
     gene            2908..3879
                     /locus_tag="PLS88_07920"
     CDS             2908..3879
                     /locus_tag="PLS88_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07309.1"
                     /translation="MNIDLITKRSRMVSIVREFFISRDYLELDTPALAPSLIPESCLE
                     VFATEYIHPTLGHIPLYLIPSPEIWMKRVIANTKRSVFQICKAWRNAESVSPIHNPEF
                     TMLEYYGVGFSSKDNIKLTEELFEALASSETPSQARPPFRVMSMKEAWAEFANADLDA
                     LGDTEAMREACRQRGLLVPQSANWEDAFHILFLSIVEPSLPKDKPLILDLYPSGIECL
                     AADIAGTRYKERWELYVEGIEIANCFTEMDNPEAVDRYMESQSNKKKESLVPHIVDKT
                     YSSIFDDFPHCSGVALGFERLMMVLCGKKSIEDVMIFPFSSFFGLDK"
     gene            4027..4590
                     /gene="efp"
                     /locus_tag="PLS88_07925"
     CDS             4027..4590
                     /gene="efp"
                     /locus_tag="PLS88_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOU07310.1"
                     /translation="MIRGGDIGIGTCLIINGIPHIVVEREFVNPGKGAAFARVRAKNL
                     KNGYVVTQTIKTADSVEDAQVDLVDCQYQYSDDESFHFMNNETFEQFEIPITAHEDRA
                     PYLKEGETYTIVMWEGEPIDIKIPYKMIFTVAESENYVRGDTVSGATKPVRTETGLVV
                     RVPLFIKQGEKILINTETNEYVERVNE"
BASE COUNT         1340 a          933 c         1154 g         1289 t
ORIGIN      
        1 gaatttgtcg atattgctga gaatctagcc cgtgacaggg cgaagaagct ttttggtgca
       61 gatcatgcca atgtgcagcc gcattcgggg agtcaagcaa atatggccgt tctctttgca
      121 gcccttaacc ccggtgacac aattatggga atgagcttag ctcatggtgg acacttgtcg
      181 catggagcac cagtatcatt ttcgggtaaa ttctacaaag tggtgagcta tggcgtgaat
      241 cctgatactg agctcataga cttcgatcag gtagcattcc ttgctcgtca acataaaccg
      301 aaattaataa ttgctggagc tagtgcctat tcgcgcataa tcgattttga cgccttccgg
      361 aagatcgcag atgaagttgg agccaacctt atggtcgata tggctcatat tgctggcata
      421 gtagccgccg gcagacaccc cagcccaatt gcgactgctc attttgttac aactacaacg
      481 cataagactt tgcgaggtcc acggggaggc atgatcctag tgggcagaga tatggaaaat
      541 gatcgtggca ttatgaatgc taagggagat gcacttcgca gatggtctga ggttatcgat
      601 agcatggtca tgccaggaat tcaaggtggg ccattgatgc atgtcattgc tggtaaagcg
      661 gttgcgttta aggaagcgca gaagcctgaa tttgtggagt acattgatca tgtgctttac
      721 aatgcaaaag tgatggcaca ggctttaacc ttgggaggag ctcgaatagt atcgggtggc
      781 actgacaatc acctcatgct tgtcgatctg actcctcttg gaataaacgg tcgggatgcg
      841 gagaagtggc ttgatgaagc aaatataacg gtgaacaaga attcaattcc ttttgataag
      901 aaaagcgcat ttgtgacgag tggtatacgc atagggacgc cagctattac gactcgaggt
      961 atgggacctt ctgagatgga acagattgct tcttttatta tggaagtatt gaaatccaaa
     1021 ggtgatgctc aggtaattgc gcgcataaag caggaggttc tagctcttac ttcacgcttt
     1081 cctctgctat aaagcgtaaa tacagaaaat ggagcgccca gagctcgatg aggaagctaa
     1141 taggcgagca gcgtattggc ttctgctgaa tgcaaaggaa caaggttttt tttcttatgg
     1201 attgctttca caaactgcat gggagcaggt tgtggaatcg caaggcgcta tagaagtaga
     1261 aagcagtgaa gcgaagcagg taaatcgaga ttccttgaga tcaggggtag cggagacaga
     1321 gcaactacag gcagagagta gtgcagtcaa ggtactgaag gagcattatc atcttttaga
     1381 tgcgcgagcg atgttagtgg tggcgcttcg gtatgcaccg gatatcgagg ctgaggcggc
     1441 ggcaggccgc gcggagcgcg ggctgccgcc tctgccactt gcttgctatg gccaaccagc
     1501 agcgaaggtg gcaaggtttg cgcgcggcaa ctggtataga gagattatgg atcgtctcgg
     1561 ccattgtgct gttgcaacct ttgaagcaat tacaagagag aggcttgcag aaagtcttga
     1621 tcttgaagga aaaccgccta gccgatggcc acgatttgtg aattcgagat ttcccgaaaa
     1681 agccctcgcc ctcgctgctg ggcttgggac gattggtcga aacagccttc ttatagctaa
     1741 aaaaacaaat gattcggata tcttgaattt gcttgatttc caagaacatc taataaaaag
     1801 cgacagtcat aatgcatgta taatgaattt gccgatttgg tcttctgcgg ttgtgattgg
     1861 cctgatgctt ctgcccttcg atgtaaaaat ggaaaaactt gaggacaagg attctcctag
     1921 aggcatagct ccttcatttt ttgctgtctg tagcgattgc aggctttgta tcgatgcttg
     1981 cccaagtagg gctttgagta tttcatctat tcctggcttc gagcggtatc tctgtattca
     2041 aaattattca tcgatcgagg gtaatctgcc ggatttcatc aataattcct ggaccgacca
     2101 gctctatgga tgcgatcttt gtcttgaggc ttgcccatac ttcgagcccg atgaagaagc
     2161 gcatgtaaat catggtcgaa taggtggttt ttttgatgct gggtcgttgg caaatttaaa
     2221 cgatgagcaa atacgagttt tgtttaaggg ctcggctctc gatcaaaagt ggatttcgcc
     2281 taaagcactt agaagaaatg cagcgataat tgccaaaaag gcttgatata ttgaggaatg
     2341 aatacgttaa atattgacat actatgctaa ataatattta attatgcaat attttgcagg
     2401 agagtgtaat atgaagaaat ttattgcagt attcgcaatc atatctattg cattttttgc
     2461 atgtactcct aaaaaagctg acaaggacaa ccagactagc aagaatattt cgagcattga
     2521 tcctgaggag atcaaagata taacaacatg gaaggccgcc tacgacgatc ttatcggtgc
     2581 ttattcaagc gcagtttcgc agctcaatgg cggaaatctg ggaacaggcg caaagatcga
     2641 agaactagga aagcgcgctg aggcacttaa ctcagtggca gagaccatta aatctggact
     2701 ttcggttcag gaacaagctg attttgctgc ggtaatcgaa gaatataaag aaaaattcaa
     2761 tcaggcagtc aattctaatt gacaaagccc acaataggca gcagctaaaa gcagcccgcg
     2821 ataagcagca atgaagaatt gaattctaag ctcagcctgc ccaatgcgta tatttcattt
     2881 cgttaagggc agtctagaat agtatatatg aatatagatt taataaccaa gcgctctcgc
     2941 atggtgagca ttgtacgcga gttcttcatt tcgagggatt atctcgagct agacacgccg
     3001 gcgcttgctc cttctctcat accggaatct tgcttggagg tctttgctac agagtatatt
     3061 catcctactc ttggccatat accgctctat ctcattcctt cgccagaaat atggatgaaa
     3121 agagtcatcg ccaatacaaa gagaagtgtt tttcaaatct gtaaagcatg gcgaaatgcc
     3181 gaatcggttt cgcccattca caaccctgag tttacaatgc ttgaatatta tggcgtgggt
     3241 ttttctagca aagacaacat caaacttacg gaagagcttt tcgaagcctt agctagctcc
     3301 gaaacccctt ctcaagctcg tccgcctttt cgagtgatga gcatgaaaga agcatgggcc
     3361 gagtttgcca atgcagatct agatgcgctt ggcgatacgg aggctatgcg cgaagcttgc
     3421 cgacagcgtg gactattagt acctcaatct gcgaattggg aagatgcctt ccatattctt
     3481 tttcttagca tagtagaacc ctctcttcct aaggataagc cacttatcct agacctatat
     3541 ccttcaggta tcgagtgtct tgctgcggat attgctggca ccagatacaa agagcgatgg
     3601 gagctctatg tagaaggtat tgaaatcgca aattgtttta cagaaatgga taatcctgag
     3661 gctgtcgatc gatatatgga gtcgcagagc aataaaaaga aagaatcttt agtgcctcat
     3721 attgtcgata agacatatag ctccattttt gatgactttc cgcattgttc tggagttgct
     3781 cttggctttg agcgcctaat gatggttttg tgtggcaaaa aatcgatcga agatgtgatg
     3841 atttttcctt tttcgtcttt ttttggatta gataaataga aaaaattctc taaggaatgc
     3901 cgaatatcaa aataaagtct agaatagttc tgtaatggaa atagtcacaa gatgctttag
     3961 tctttacata gatcaagatt ccaagctagt atagattgat taaatgcttt ttttgaggga
     4021 aaaattatga ttcgtggtgg tgatatcggg atcggaactt gtctcattat caatggcata
     4081 ccgcatatag tcgtagagcg agagtttgtc aatccaggga aaggcgctgc ttttgctcgt
     4141 gtgcgcgcga agaaccttaa aaatggatat gtggttactc aaacaatcaa aaccgctgat
     4201 tctgtagaag atgcccaagt agatcttgtc gattgtcaat atcaatatag cgatgatgag
     4261 tccttccatt ttatgaacaa tgagacattc gagcagttcg agatacccat aacggctcat
     4321 gaagaccgag ctccttattt aaaggaagga gaaacctata caatagttat gtgggaaggt
     4381 gagcctatag acatcaaaat tccctataag atgattttta ccgtggcaga gagcgaaaat
     4441 tatgtgcgcg gtgataccgt atctggagcg acaaaacccg taaggaccga aactgggctt
     4501 gtagtgcggg tgcccctctt catcaagcag ggcgagaaaa tccttatcaa tacggagaca
     4561 aacgagtacg ttgagcgcgt caacgaataa ggccgacgat tcattctgtc agctagatcc
     4621 gctagtaaat aggacagtag atttcgctta ccgtggcact aagctcaaat tcgagctatc
     4681 tcattcgctt ttttcttctt ttgatatcga tgaagg
//
LOCUS       DAOSOB010000089         4545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_19124_length_4545_cov_73.555234, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000089 DAOSOB010000000
VERSION     DAOSOB010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19124_length_4545_cov_73.555234"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="PLS88_07930"
     CDS             <1..1454
                     /locus_tag="PLS88_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOU07311.1"
                     /translation="QGRTKKMVIQDKGQLEKLFESQTERRWELALTGPEERIAKLKRL
                     RSAILARSDELYMALKSDFSKPAFEAWLTEVFPCIEEIDFAVGHLKAWMKGRRAKGTL
                     ILPLASTRVRYEPKGRVLIMAPWNYPFQLLIAPLISAVAAGNCVIAKPSNKTPHTAAF
                     IADLIAGVFEPQEVAIVEGPGSTLGDLLLELPFDHIFFTGSPSVGVKVGEAAARVHAG
                     LTLELGGKSPAILLRGANLKQASRRIAWGKFLNAGQTCIAPDYLFCPSELVGEFATEL
                     RQAVESFYGKTEEDRQASFDFARIVDKAACARLERIVKDAISKGAKLEFGGRFDVDNR
                     YASPTLLTGVQIDMAIMAEEIFGPILPVLAYDSLDEAIEFIRSRPKPLSLYILGRDKK
                     SAERLLARTTSGSVGINDLILQIENLDAPFGGIGMSGTGSYHGFYGFKTLSHERTVLN
                     QGPISAVEKLYPPYNTPAQKRLLGALKRIKNIS"
     assembly_gap    1503..1602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1603..2462)
                     /locus_tag="PLS88_07935"
     CDS             complement(<1603..2462)
                     /locus_tag="PLS88_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOU07312.1"
                     /translation="MDNTLLPDSIDHFIKDALTPEFWSKAFTILVTAILILVFFRILR
                     IILDRGMKKSIPEQKAQLILKAIRYTGWVIAIVSVLQSVGVNLSALLGAAGIAGIAVG
                     FAAQTSISNLISGIFLVFEKPFQISDVIQIDDISGTVMSIDLLSVKLQTFDNRFVRIP
                     NESIIKTNVINLTRFPIRRLDIYVNVSYSTDLKKVTDVLRDLASKNIYVLDNPSPLIL
                     IDKYDIPGVSILLGVWFERSNLIEIKNTILMDIQERFAEEGIEIPYPKRDVYIKGIVE
                     RESSERKLSE"
     gene            complement(2476..3321)
                     /locus_tag="PLS88_07940"
     CDS             complement(2476..3321)
                     /locus_tag="PLS88_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07313.1"
                     /translation="MWNYFVPKESQWYCWKLDGASLWMRKNGEEWRFAASSIPFKQIQ
                     NDSEGPLSIDAPKDLPIALAVAAGKKVALRPHPSTAPYLVSARNEVKIYPSTEAQFDI
                     ALPPIFHIELEDGSSLFECFPFVTPYTWFGDKTSGDLCLSLPIELDPQCKNEQGYFKE
                     IGPNTNISLDPIMQESLRRAAQYLSCRSLIHCKIIVKNKSKEPLDVKRLVIFTSLMNV
                     YLKQDNLISDTVIITATADGSLQTNIDDSAYKNLTKIHIAPKTGLNELLVMKGVSFLR
                     SIAGL"
     gene            complement(3391..>4545)
                     /locus_tag="PLS88_07945"
     CDS             complement(3391..>4545)
                     /locus_tag="PLS88_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOU07314.1"
                     /translation="RLLMIPGPIEFTPEVLAEMAKPTLSHTDPQFIEEFGQALEKMRK
                     VWLAPNGQPFIIAGSGTLAMEIAAVNITEPGDNVLILHTGYFSERMADIFKLHGANVD
                     IVQSTVGDLPQIDEVRKKLASKKYKILSITHVDTSTGVRADVKALAAAAQETDTLVVV
                     DGVCAVAGEELRMEEWGVDIAFTASQKAIGVPPGLALLVASKKAIEAFKSRKSPVGAY
                     YCDWQYWLPVMEAYEGRKSAYFGTPAVNLVRALNISLGQILDEGMEARFARHARNAAA
                     FRAGLSAIGLGFVPIRKEIMANTLSAVYYPEGTDASILPLIAKNGAMLAGGLLPAIKT
                     KYFRIGHMGMSDASEILATLGAIEQGLSTAGYSFEPGASLSAAQAALMQP"
BASE COUNT         1151 a         1015 c         1111 g         1168 t
ORIGIN      
        1 agcaagggag aacaaagaaa atggtcattc aggataaagg gcagcttgaa aagttatttg
       61 aatcgcaaac tgagcgacgt tgggaactag cccttacggg gcccgaagag aggattgcca
      121 aattgaaaag gctccgctcg gcaattttgg caagaagcga tgaattatat atggcgctaa
      181 agtcggactt tagtaagcca gcattcgaag cctggctcac cgaagttttt ccttgcatag
      241 aagaaatcga tttcgcagta ggtcatctta aagcatggat gaagggaaga cgggcaaaag
      301 gcactctcat cctgcctctt gcttcgacgc gcgtgcgata tgagccaaaa gggcgggtcc
      361 taataatggc gccatggaac tatccatttc agcttctcat cgcgccactc atctcagcag
      421 tagctgcagg caactgcgta atcgcgaagc cctcgaacaa gacaccacac actgccgcct
      481 ttatcgcaga cctaatcgcg ggagttttcg agccccaaga agtagcgatt gtcgagggac
      541 caggcagcac acttggcgat ttattgctcg agttgccatt cgatcacata ttctttactg
      601 ggagcccttc ggtcggagtt aaggtgggcg aagccgctgc gcgcgtgcat gcagggctca
      661 cgctggagct tggtggcaaa tcgccagcca ttttgttgcg aggagcaaat ctaaagcagg
      721 ccagtagacg catcgcctgg ggcaaatttc taaatgctgg ccagacatgc attgcgcccg
      781 attatctttt ttgcccttcg gagctggttg gagaatttgc gaccgagttg cgccaggccg
      841 ttgaatcgtt ctatggaaaa acggaagagg atagacaggc ttcttttgat tttgcacgca
      901 ttgtagacaa ggctgcctgc gctcgccttg agcgaatcgt caaagatgca atttccaaag
      961 gagccaagct cgaattcggc ggtcgcttcg atgtggataa tagatatgcc tcgccgactt
     1021 tgctcacggg agtgcaaatc gatatggcca taatggcgga ggaaatcttt ggcccgatac
     1081 tgccggtgct tgcttacgat tcactcgacg aggcgatcga gttcataaga tctaggccaa
     1141 aaccgctttc tctctacatt ctaggaagag acaagaaaag cgccgaaagg ctcttggcca
     1201 ggactacctc gggtagtgtc ggcataaacg accttatttt gcaaatcgaa aaccttgacg
     1261 ctccttttgg tggcataggg atgagcggta caggaagcta tcatggtttc tatggtttca
     1321 agaccttatc gcacgaaaga acagtgttaa atcagggacc cataagcgca gtcgaaaaat
     1381 tatatccgcc ctataatacc ccagcgcaga aaaggctgct tggagctctg aaaaggatca
     1441 agaatattag ttagtagttc gagaaaatca gaatctagca gggataaaac gatactttag
     1501 ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttttcgct caactttctt
     1621 tcgctcgact ccctttcgac aatacccttg atatagacat cgcgctttgg atacggtatt
     1681 tcgatgcctt cctcggcaaa tcgttcttga atatccatga gaatcgtatt ctttatttct
     1741 atgagatttg acctttcgaa ccatacacca aggagaatgc tgacgcccgg aatatcgtat
     1801 ttgtctataa gaatgagggg agatgggtta tcgagcacgt agatgttttt ggatgcaagg
     1861 tctcggagaa catccgtgac ttttttcagg tcggtgctat aggagacatt cacatatata
     1921 tcgagcctac gtattggaaa gcgggtaagg ttgataacat tagttttgat gatgctttcg
     1981 ttcggtatgc gcacaaagcg gttatcgaat gtttgaagtt tgaccgataa aagatcgata
     2041 gacattacgg ttccggatat atcgtcgatc tgaataacat cgcttatctg aaagggtttt
     2101 tcaaagacaa gaaatatgcc gctgatgagg ttcgaaatcg aggtctgtgc cgcaaatcct
     2161 acggctattc ccgcaattcc ggcggctccg agcaatgccg aaagattgac tcccacactc
     2221 tgcaagaccg acacgatagc gattacccag ccagtataac gtatcgcctt gaggatgagc
     2281 tgcgcttttt gctcaggtat gctctttttc attccacggt caaggattat ccgtagtatt
     2341 ctgaaaaaga cgaggataag aatcgccgtg actagtattg taaaagcctt gctccagaac
     2401 tcgggggtaa gagcatcctt tataaagtga tctatggaat cagggagcaa tgtgttatcc
     2461 atttcattct cctcattaaa ggccagcgat actgcgcaag aaacttacac ctttcatgac
     2521 gagcagctca tttaggccag tcttgggggc tatatgaatc tttgtaagat ttttataggc
     2581 gctatcatcg atatttgttt gaagtgaacc atccgcggta gccgtgatga tcacagtatc
     2641 ggatatgaga ttatcttgtt taagatagac attcataagg ctcgtaaaaa tgacaaggcg
     2701 ttttacatcc agaggttctt tggatttatt tttgacaatg attttacaat gaatcagcga
     2761 gcggcaggat aagtattgag ctgctcttcg aagcgattcc tgcattattg gatcgagaga
     2821 gatatttgta tttgggccaa tttctttgaa atagccctgt tcattcttac actgaggatc
     2881 gagctcgatg ggaagcgaga ggcagagatc tccgctggtc ttatcgccaa accaagtata
     2941 aggggtcaca aatggaaagc attcgaatag agaagaacca tcttcgagct ctatatgaaa
     3001 tatgggagga agagcgatat cgaactgtgc ctcggtagat gggtatattt tgacctcgtt
     3061 tcgtgccgag acaaggtagg gcgcagtgct aggatgtggg cgtagcgcaa cttttttccc
     3121 agcagcgacc gcaagggcaa tcgggaggtc tttaggagca tcgatagaca aagggccttc
     3181 cgaatcattt tggatttgct tgaaagggat cgagctagcc gcaaagcgcc attcttctcc
     3241 attcttgcgc atccaaagag aggccccgtc tagcttccag caataccatt gcgattcttt
     3301 aggtacaaaa tagttccaca tacgattcat aatatcgtat gtggaatatt ccttaaagtc
     3361 gattgggggg ggatccttat gattttgcta ttaaggctgc atcaaagccg cttgagcggc
     3421 tgagagcgat gctcctggct cgaaggaata gccagcagtt gaaaggccct gttctatagc
     3481 accgagggtt gcaagtatct ccgaagcatc gctcatgccc atatgcccaa ttcggaaata
     3541 ctttgtcttt attgcgggaa gcagaccccc ggcaagcatg gcaccattct ttgcaattaa
     3601 aggtagaatg cttgcatctg ttccttctgg gtaatagacg gcgctgagag tattggccat
     3661 tatctctttc ctaattggga caaagccaag gccaattgca gaaagccccg ctcggaaggc
     3721 cgccgcattg cgtgcatgcc ttgcaaacct ggcctccata ccctcatcca atatctgccc
     3781 caggctgatg tttagcgctc gcacaaggtt gacagcaggc gttccaaagt aagccgattt
     3841 gcgcccttca tacgcttcca ttacgggcag ccagtattgc cagtcgcaat aatatgcgcc
     3901 taccgggctt tttctcgact taaaagcctc tatggctttc ttgctagcta caaggagcgc
     3961 aaggcctgga ggcacaccaa tggccttttg cgaagcagta aaggcaatgt ccacgcccca
     4021 ttcttccatg cgaagctctt ctcctgccac tgcgcataca ccatcgacca ctacaagcgt
     4081 gtctgtttcc tgtgcggcgg cggcaagagc ctttacatcg gcgcggactc ccgtgcttgt
     4141 atccacatga gtaatgctga gtattttgta ttttttagat gcgagttttt ttcgcacctc
     4201 gtctatttga ggaagatcgc cgaccgtgct ttggacaata tcgacattgg caccgtggag
     4261 tttgaagatg tccgccatgc gttcgctgaa ataacctgtg tgcaagataa gtacattgtc
     4321 tccgggctct gttatgttta ccgcagcaat ctccatggca agcgtaccgc ttcctgcgat
     4381 tatgaatggc tgaccattag gggcaagcca gactttgcgc attttttcga gcgcttgacc
     4441 gaattcttca atgaattgcg gatcggtatg gctcaaggta ggttttgcca tttcggccag
     4501 gacttcgggg gtgaattcta tggggcctgg aatcatcaat aagcg
//
LOCUS       DAOSOB010000090         4447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_19662_length_4447_cov_78.733379, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000090 DAOSOB010000000
VERSION     DAOSOB010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19662_length_4447_cov_78.733379"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..317
                     /gene="rhaM"
                     /locus_tag="PLS88_07950"
     CDS             3..317
                     /gene="rhaM"
                     /locus_tag="PLS88_07950"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOU07315.1"
                     /translation="MKRYAFVMQLKPGNEAEYKRRHDEIWPELKDELRKAGISNYSIY
                     LDPETLRLFAIQNLADDEKSDSLSRLPIMHKWWDSMKDLMETNADNSPKVWALKKMFH
                     MD"
     gene            336..1523
                     /locus_tag="PLS88_07955"
     CDS             336..1523
                     /locus_tag="PLS88_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOU07316.1"
                     /translation="MNLDSIIRLSRRYGADSRYVLAGGGNTSLKQDNIMAVKASGVSL
                     GTIDKRGFVLLSLPQLIDIFEAVLPSSAAEREKIVLERLLASRLPEQTMRPSVETLLH
                     ALFPFKFVVHLHPALVNGLLCSVEALEKAPELFGEEAEILPYITPGYTLADRVRTLFR
                     TRKSMGKGPIKILFLQNHGVFVAADNADEIGDLYRYIFNTLEKWVKPLPVDDDTYSVV
                     SKAKSELLSEDARIVMEAISSEFNVRFFFTSNSLLDYFLSSPEAFIPLSKPFTPDHIV
                     YAGAWPLYLDQSTARNIKHIESSIHGYISDHDELPKIIAIQGIGVVGLGKDHKAAERA
                     CLLFQDAAKIAWYAQSFGGVHPMEQKDADFIRGWEVEKFRSGVAASGLTTPSSESAPI
                     LKK"
     gene            1552..2808
                     /locus_tag="PLS88_07960"
     CDS             1552..2808
                     /locus_tag="PLS88_07960"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOU07317.1"
                     /translation="MPRTEQEYKAARETYAELGIDTDQAIETLASIPLSIHCWQGDDV
                     IGFDGAKSLSGGILSTGSYPGRARNAEELRSDAVFAFSLIPGKKRFNLHAMYAETGGA
                     KVDRCDLEPRYYEGWIEWAKQQHIGLDFNPTFFSHPLAESGWTLAHPDNDIRDFWIRH
                     GKASRRIAQSIADTLKDHVVNNLWVPDGYKDMPADRLGPRLRLKESLDAIYSEKLPND
                     RVLDSVESKLFGIGSEAYVAGSHEFYLGYASQKAIGLCYDMGHFHPTENIADKISSTL
                     LYVPYLIIHLSRGLRWDSDHIVVWNDSLTDVCREIVRMHVWDRVHLALDYFDSSVNRI
                     SAWINGARSTQRALLYAFLEPIEQMRAAEEQGDFGTRLSYIEESSSLPFSAVWHHYCE
                     TENVPYGPSWLEDVKDYEKKVLAKRP"
     gene            2920..3864
                     /locus_tag="PLS88_07965"
     CDS             2920..3864
                     /locus_tag="PLS88_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU07318.1"
                     /translation="MANQENIDIKEKRYSRPNLKMVEQNLHTTSKTDNFDIEKEINGN
                     RYAEIVTQSNKPLFTFLRVSIREDLRFTLPRIIMRTAKTMRRIPRTFIIKNEKELDQY
                     VFETISLWARSLIRITKMDLEVRGAEKILPDKTYLFASNHRSPADIPLLFVCIPQPAA
                     FVANDLFRKIPALSYWMRASGSVFVDQGNTQSEVRALKAMEKRLKKGRSLILFPEGHM
                     HQGKGIGEFSRGGLFAAVLCGVPIVPICTYGTDKVMRPGSFHINPYRHVVVEFGDPIE
                     TKNLDRADKKRIHLVVHDIIKSMRTALEQEFSSSRKKN"
     gene            complement(3896..>4447)
                     /locus_tag="PLS88_07970"
     CDS             complement(3896..>4447)
                     /locus_tag="PLS88_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07319.1"
                     /translation="WLKFDVLSEKIGIKEFLRKGNVRYSASKLIGVFLYWIVMLFALA
                     KAASVLDKRAAETISSWLYAAIPSILATIITATIGIFIANFLSNFVMTIMNNAGLSSA
                     TLVKRLIRVVGYIIVVIMVVEQLGFESIVNVLLLLLIGSVALGVAIAIGLGCKDIARQ
                     YVESFLRSLRERERAAHRTDLEG"
BASE COUNT         1326 a         1026 c          968 g         1127 t
ORIGIN      
        1 aaatgaaacg ctatgcattt gtcatgcagc tcaaacctgg aaatgaagcg gaatacaaac
       61 gccgacatga tgagatatgg cccgaactaa aggacgagct tcgtaaggca ggaatttcga
      121 attattcgat ttacttagat cctgaaacat taaggctttt tgcaattcaa aacctagctg
      181 acgatgagaa aagcgattcc ttgtccagat tgccaataat gcacaagtgg tgggattcga
      241 tgaaagacct catggaaacg aatgctgata attcacctaa agtttgggcg cttaaaaaaa
      301 tgttccatat ggactgaata catgaaggtg tgtgcatgaa tctcgattct attataaggc
      361 tttctcgccg ctatggagcg gactcccgat acgttttggc tggaggagga aacacttctc
      421 tcaagcaaga taatatcatg gcggtaaagg cttcgggagt ttcattggga acaatcgaca
      481 agagaggttt tgtattgtta tcgcttcctc agctcatcga tattttcgag gctgtacttc
      541 cttccagcgc ggctgaacgt gagaaaatcg tgctcgaaag gcttcttgct tcacgcttgc
      601 cggaacagac gatgcggcca agcgttgaga cgctgctgca tgccttattt ccttttaagt
      661 tcgttgtgca tctgcatcct gcgctggtca acggtctatt atgttcggtc gaagctctcg
      721 aaaaagcacc tgagctattt ggggaagaag ctgaaattct cccttatatc acgcctggat
      781 atactcttgc agatagagtc cgaacattgt ttagaacacg caaatccatg ggcaaaggcc
      841 caattaaaat acttttcttg cagaatcatg gagtatttgt cgctgcggat aatgctgatg
      901 aaataggtga tctatatagg tacattttta atactctgga aaaatgggta aagccattgc
      961 cagtggatga tgatacttat tctgtagtaa gcaaggcaaa gagcgagctt ctatcagagg
     1021 atgcgaggat agtgatggaa gctatatcct cggaatttaa cgtaagattt ttctttacgt
     1081 caaattcgct tttggattat ttcctaagct cacctgaagc ttttattccg cttagtaaac
     1141 cttttacacc cgaccacatt gtctatgctg gcgcttggcc tctatacctc gaccaatcaa
     1201 cagctagaaa tataaaacat atcgaatcat caatacatgg atacatctca gaccatgatg
     1261 aactgccaaa aattatcgcc atccaaggaa tcggcgttgt cggcctagga aaagaccaca
     1321 aggcagccga acgcgcctgc cttctctttc aagatgccgc aaaaatcgcc tggtacgctc
     1381 agtcatttgg aggcgttcat ccgatggaac aaaaagatgc ggattttatc cgaggttggg
     1441 aagtagaaaa attccggtcg ggggtagctg ccagcgggct gaccactcct tcttcggaaa
     1501 gtgctcctat actaaagaaa tgatttctag ctatgaaggg aggagaattc aatgcccaga
     1561 actgagcaag aatataaagc agcacgagag acctatgctg aacttggcat cgatacagat
     1621 caagccatag agactctcgc ctctatacca ctttccatcc attgctggca gggagacgat
     1681 gtaataggct tcgacggagc taaatcactc tctggtggca tactctccac aggaagctat
     1741 cctggtcgag cgcgcaatgc cgaagagctg cggtcagacg ccgtcttcgc cttcagcctt
     1801 atacccggta aaaagcgctt taacctccat gccatgtatg cagaaacagg tggcgccaaa
     1861 gtagatcgtt gcgatcttga gccgcgctac tatgaaggct ggatagaatg ggcaaaacaa
     1921 cagcatatcg gcttggattt caatccaact ttcttttctc atcctcttgc agaaagcggc
     1981 tggactttag ctcatccaga taatgatatt cgagattttt ggattcggca tggcaaggca
     2041 agcagacgca tagcccagtc catcgccgat acactcaagg accatgttgt caataatctc
     2101 tgggtgcctg acggttacaa agatatgccg gccgaccgcc ttggtccgcg cttaagactc
     2161 aaagagtctc tcgatgccat atattctgaa aaactaccaa acgaccgtgt actcgacagc
     2221 gtagaatcca agctctttgg tataggctca gaagcctatg tagcaggttc ccacgaattc
     2281 tatctgggct atgcgtccca aaaagccatt ggtctatgct atgacatggg ccacttccac
     2341 cccaccgaaa acatagcgga caagatttct tcgactcttc tgtatgtgcc ttacctgatt
     2401 atccacttga gcaggggatt gcgttgggac tcagatcaca tagtcgtctg gaatgattcg
     2461 ctcacagatg tctgtagaga gatcgtcagg atgcatgtct gggatagagt ccatcttgcc
     2521 ctcgattact tcgattcgag cgtaaatcgc atctcggcat ggatcaacgg ggcacgcagc
     2581 acccaaagag ccctactcta tgcttttctt gagcctatag aacagatgcg cgctgcggaa
     2641 gaacaaggag actttggaac aaggctctcc tatatcgaag aatcaagctc tctgcctttt
     2701 agcgctgtat ggcaccatta ttgcgagaca gaaaatgttc cttatggtcc cagttggctc
     2761 gaagatgtaa aagattacga gaagaaggtc ttggcgaaaa gaccatgact ttacagaagt
     2821 agataatccc aataagctaa acctatacag atattctcgt tcttgactaa tagtgtccaa
     2881 aacctagata ttataatata tagggtagaa aacgaacgca tggcaaacca agagaatata
     2941 gatatcaaag agaaaaggta ttcaagacct aatctcaaaa tggtggagca gaacctccat
     3001 acgacttcta agactgacaa ttttgatatc gaaaaagaaa taaatggaaa caggtatgca
     3061 gaaatcgtta cccagagcaa taagccgctt tttacctttc tccgcgtttc cattagagaa
     3121 gacctcagat tcacgctgcc tcgtattatc atgcgaacgg caaaaaccat gcgtcgaatt
     3181 ccgcgcacct tcataatcaa aaacgaaaaa gaactcgacc aatatgtatt cgaaactatt
     3241 tccttatggg cacgcagctt aataagaatt actaaaatgg atctcgaggt gaggggcgca
     3301 gaaaaaatcc ttcccgataa gacctatctt tttgcttcta atcatcgatc gccagcggat
     3361 atacctctgc tcttcgtttg cattcctcaa cccgctgcct ttgtagccaa tgacttattt
     3421 agaaaaattc ctgctctctc ttattggatg cgcgcaagcg gctcggtctt tgtcgatcaa
     3481 ggcaatacac aatcggaagt gcgcgccctg aaagccatgg aaaagcgttt gaagaagggc
     3541 cgaagcctca tattattccc tgagggacat atgcaccaag gcaaaggaat cggtgagttc
     3601 agccgaggtg gcttgtttgc agcagttcta tgcggcgttc caatcgtacc tatatgtacc
     3661 tatggaacag ataaagtcat gcgacctggc agttttcata tcaatccata tcgccatgta
     3721 gttgtagaat tcggtgaccc catagagacc aagaatttag atagagcaga taaaaagcgc
     3781 atccatcttg tggttcatga catcataaaa agcatgagaa cagctctcga gcaggaattc
     3841 tcatcctcaa gaaagaaaaa ttgagcctac catacaccaa aagaggcata agcatttatc
     3901 cctcaagatc ggttctgtgc gcagcccgct cgcgctcacg aagcgaccta agaaagctct
     3961 ccacatactg tctggcaata tccttacagc caagaccaat cgctattgca actcccagcg
     4021 ctacagagcc tatcagcaat aaaagaagaa cattcacaat cgattcaaaa ccgagctgtt
     4081 cgacgaccat gatgacgaca atgatatagc ccacaactct aattagtctc ttgaccaatg
     4141 ttgctgagct aagccctgca ttgttcatta tcgtcattac gaaatttgaa agaaaatttg
     4201 caataaaaat gccaatcgtc gcagtgatta tagtggctag aattgatgga atcgcagcat
     4261 ataaccatga agaaatcgtc tcggcagccc ttttatcgag tactgatgca gcttttgcca
     4321 aagcaaagag catcacaatc caatatagaa aaacaccaat gagctttgaa gcagaatacc
     4381 taacatttcc cttgcgcaaa aattccttga taccgatctt ctccgaaagc acatcgaact
     4441 tgagcca
//
LOCUS       DAOSOB010000091         4430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_19755_length_4430_cov_66.466286, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000091 DAOSOB010000000
VERSION     DAOSOB010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19755_length_4430_cov_66.466286"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            408..1646
                     /locus_tag="PLS88_07975"
     CDS             408..1646
                     /locus_tag="PLS88_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOU07320.1"
                     /translation="MKSRKTRAIFVMVFFVASLIVLGAQQKTTLSVLWFNDANESDVF
                     LETMKDYQKAHPNIVLDMQVIPFNDYENKLRMMIAGGNPPDVARLASAHVPLFATSLE
                     PLSGKPEFDELAKSYFDSSLALGMDENGRIMAMPTEATANGMIVNKTYFKNAGIDVDK
                     LSLTWTWDQWVDAMKKVLKANPKAKYGITVDFSTHRFATFLYEAGGRFLSSDGKSMNF
                     NTPESLDALKFFKMLHDEGLAPKSVWMGSENPQELFQSGLVACHVGGSWLINAYNKNI
                     KDFEWAAVRMPKRKINSSVPGGKFIGTFKGSPHKKEALDLIAVFSDKAHNEQYCKDTF
                     NLSARKDANIKYAARSNDFAVFTAELNVTPPYTADDWKSDAVAKVNSYAREQIVEGLL
                     GKQTMEQTAANIQAKGNSYF"
     gene            1789..2682
                     /locus_tag="PLS88_07980"
     CDS             1789..2682
                     /locus_tag="PLS88_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU07321.1"
                     /translation="MKAKQRSHGTFEQTLVPLLFLAPNLLIFSIFIIVPAIMGLRMSF
                     FDWSILEGSRFIGFQNFKNLSHDSMFWLTLKNTLVYVVAVVPLLTIFALALALPLSKE
                     DKGMGIFRVIYYIPTMLSMIIVGISWRWLLGYDLGIINYVISLAGGKPVPWLTNGTMA
                     NISLIFVTVWTRVGYFMMMFVGGLQAIPATYYEAAKMDGAGKSTIFSRITLPLLSPTI
                     LVVVVLATIEAFKAYELIFVMTQGGPGTSTKFIVQYIYQVAFQEDKMGYGAAMSIILL
                     VIIGIFTALQFSIQKEEYTNE"
     gene            2675..3484
                     /locus_tag="PLS88_07985"
     CDS             2675..3484
                     /locus_tag="PLS88_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU07322.1"
                     /translation="MNKVLKYILLIVVSGIFLFPILWVIISSFKPQSELFTYPLTILP
                     NKATIENYVNAFASGDFITYYTNSIFVSIVATLLTITINVMAGYALSKFYFRGRDLIF
                     YIMISTLMIPLQAIMIPIFLLLKKLGLLNSLWGIIIPPAATPTGVFLARQYLQTIPNS
                     YIEAARMDGASELRIFLSIIVPLAQPIIATIAIFSFMWRWNDFLWPFIVISSKRKMTL
                     QLALANFVGQFQINWGNLLAMTVVTMIPMIVVFLALQRYFVQGLSAGGVKA"
     gene            3487..>4430
                     /locus_tag="PLS88_07990"
     CDS             3487..>4430
                     /locus_tag="PLS88_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOU07323.1"
                     /translation="MLYVIKNLSAYHLEGSKAIFAGENGQLEVVIHTSNLFSIFYIFD
                     ESIVPPKINKPHAALLSFNNIGDALSWDSVVEKDNQYELIHGSTRILVDKATSRVDIY
                     KNDVLFHGGEIGHKDLVVPQYPLRVQLGDMQSNARQAIYGKFNFRLEEADAFFGLGEK
                     TGKLNKRNRLFKLFNRDALGYNAETSDPLYKSVPFFIKANRVLNNICGFYFPNLQVDE
                     VNFGVESEFYYDVVLSSGPFGYYVITGDHYREVLSTYCKICGLPALPPLFSFGYLTSS
                     MGYTEPDIAQEKILEFFARIEQEDIPCEGMYFSSGYAKA"
BASE COUNT         1263 a          871 c          965 g         1331 t
ORIGIN      
        1 tacggcccta ggatgcttcg tgctccttcg tatgtcagat cggagaagag gctgacaatg
       61 ccaagcagca ctagaaactt gtaagcagct tttttcctgt tttgcatatt gatttccttt
      121 ttgtataata ccccgacaag ggaatcatgg ccagatgtca atagaattat cgaatcatac
      181 aatagtgagg ggtgaggcaa aggcagagtc gagaattgat gtgcacaaca tgtttggcca
      241 aagctaatca aggagaattc tttcggtatc gaaaatttga gtgcacagca gttttgtgca
      301 aaggcagtag ggttggcaat tttagaaact tgacaaatat gcaaagtggt tttatcatca
      361 ttattgtcaa aacgttttga caataaaagg ttgtaggagg ctcgattatg aaatcaagaa
      421 aaacaagggc gattttcgtt atggttttct ttgttgcttc tttgatcgtc ttgggagccc
      481 agcagaaaac gactctttcc gtgctttggt tcaatgatgc caatgagtcc gatgtatttc
      541 tcgaaacaat gaaggactat cagaaggctc acccaaacat cgtcctcgat atgcaggtca
      601 tacctttcaa tgattatgag aacaagcttc ggatgatgat tgcaggcgga aatcctcctg
      661 atgtcgcaag attggcgagc gcgcatgttc cgctttttgc tacgagtctc gaacccctat
      721 ctgggaagcc cgaattcgat gagctggcga aatcctattt cgattcttct ctagctctag
      781 gtatggatga aaatggtaga ataatggcaa tgccaaccga agcaacggca aatggaatga
      841 tagtaaacaa gacctatttt aaaaatgcag gcatagatgt cgataaatta tcgcttacat
      901 ggacatggga tcagtgggta gatgccatga aaaaagtcct aaaagcaaat cccaaggcaa
      961 aatatggcat aacggtagat ttttcgacac accgatttgc tactttcctc tatgaagccg
     1021 gcggacgatt tttatcgagc gacgggaagt cgatgaattt caatacccca gagtctcttg
     1081 atgcactgaa attcttcaag atgttgcatg atgaaggtct tgctcccaaa tcggtgtgga
     1141 tgggctcgga gaatcctcaa gagctgttcc aatcgggctt agtagcctgt catgtcggtg
     1201 gatcatggct aataaatgca tataacaaga atatcaaaga ttttgaatgg gcagcggtaa
     1261 ggatgccaaa acgaaagata aattcctctg tgcctggcgg caagttcatt ggcactttca
     1321 aaggctcacc ccataaaaaa gaagcattgg atctcatcgc tgtattttcg gataaagctc
     1381 acaatgagca atactgcaaa gatactttca acctttccgc acgaaaagat gcgaatatca
     1441 agtatgccgc aaggtcgaac gattttgccg tctttaccgc cgaactcaat gtaactcctc
     1501 cctatacagc cgacgattgg aaaagcgatg cggtagcgaa ggtcaactcc tacgcacgtg
     1561 agcagatagt ggaagggctg ttgggaaagc agacaatgga gcagactgcg gctaatattc
     1621 aagctaaagg caattcatat ttttgaaaaa tagtctgtta tttcaaaatt gacgtaagaa
     1681 tttatcttac gatagaatct aagatgttac ttctgctgta tgcatcgaac caggatgcat
     1741 acggcagcct ctttttatag aaaatcaatt attgaagaga aagaaaggat gaaagccaaa
     1801 cagcgatctc atggcacatt cgagcaaacc ttggtacctc tattgtttct tgcaccgaat
     1861 ttgctgattt tttcaatatt catcatcgta ccagccatca tgggacttcg gatgtcgttt
     1921 tttgattgga gcattctaga ggggtccaga tttataggtt ttcaaaactt caagaatcta
     1981 tcgcatgatt cgatgttttg gcttactcta aagaatacac ttgtctatgt tgttgctgtc
     2041 gttcctcttt taacaatttt cgctttagcc cttgcgttgc ctctttctaa agaagataaa
     2101 ggcatgggta ttttccgtgt catctattat ataccgacca tgctgagcat gattattgta
     2161 ggcatttctt ggcgatggct ccttggatat gatcttggca ttatcaatta tgtaataagt
     2221 ctagctggag gaaagccagt accatggctt accaatggta caatggctaa tatcagcctc
     2281 atatttgtga cggtttggac aagggtaggc tattttatga tgatgttcgt tggtgggctt
     2341 caagctattc cagcgactta ttatgaagcc gccaagatgg acggagcagg caagtcgact
     2401 atttttagcc gtattacgct tcctcttttg agccctacga ttctcgtagt tgtagtgctt
     2461 gcaactatcg aggctttcaa ggcgtatgag ctgatatttg tgatgactca gggtggaccg
     2521 ggaacatcca caaaattcat cgttcagtat atctatcagg ttgcttttca agaggacaag
     2581 atgggatatg gtgcagctat gtcaataatt cttctcgtta tcattggaat attcacggct
     2641 cttcagttca gcatccagaa ggaggaatat accaatgaat aaagtattga aatatatatt
     2701 gttgatagta gtttcaggca tatttttatt cccgattcta tgggtcatta tctcctcatt
     2761 caaacctcaa tcggagctct ttacttatcc actaactatt cttcccaaca aagcgacgat
     2821 cgagaattat gtcaatgcat ttgcaagcgg agattttatc acctattata caaattccat
     2881 ttttgtctcc atagtcgcaa ccttgcttac cataacaata aatgttatgg cgggttatgc
     2941 tctttccaag ttttatttca gagggcgaga cctgattttc tatattatga tcagcacgct
     3001 gatgattccc ctacaggcga ttatgatacc aatttttttg ctattgaaga aattaggttt
     3061 attgaattct ctatggggaa ttatcattcc gcctgcagca accccaactg gtgtattttt
     3121 ggcccgtcaa taccttcaga cgattccgaa ttcgtacatc gaagcggccc gaatggatgg
     3181 agcaagcgaa ttgcgcatct ttttgagtat cattgtacca ttagcccagc cgatcattgc
     3241 tacaattgcg atcttttctt tcatgtggcg atggaatgac ttcctttggc cattcattgt
     3301 tatctcatcg aaaaggaaaa tgactttgca gcttgctctc gctaattttg tggggcaatt
     3361 ccagataaat tggggaaatc ttcttgcaat gactgtagtg acaatgatac cgatgatagt
     3421 cgttttcctc gcgttacagc gatactttgt ccaagggctc agcgcaggcg gagtaaaggc
     3481 ataaatatgc tatatgtcat aaaaaacctc agtgcatatc atctagaagg ttctaaagcc
     3541 atatttgctg gtgagaatgg tcagctcgaa gtagttatcc atacttccaa tttgttctca
     3601 attttctata ttttcgatga atcaatagta ccgccaaaga taaacaagcc tcatgcagca
     3661 ttgctttctt tcaataatat aggcgatgcg ctatcttggg attctgtcgt tgaaaaggat
     3721 aatcagtatg agctgataca tggctcgaca aggattttgg tagataaggc gacgagcagg
     3781 gtcgatattt ataaaaacga tgtgctcttt catggtggcg agattggaca taaggactta
     3841 gtggttccac agtatcctct tcgggtacag ctaggggata tgcaatctaa tgcaaggcag
     3901 gctatatatg gcaaattcaa ttttcgtctt gaagaagcag atgctttttt cggattagga
     3961 gagaagacag gaaagctaaa taagcgaaat cgactcttca aacttttcaa tagggatgcg
     4021 ctcggctaca atgccgagac aagcgacccg ctctataaat cggtgccttt ctttataaag
     4081 gccaatagag ttctcaataa tatatgcggt ttctatttcc caaatcttca agtcgatgaa
     4141 gtcaattttg gagtcgaaag cgaattctat tacgatgtcg ttctgtcatc ggggcctttt
     4201 ggatattatg taataacggg tgatcattat cgagaagtct tatccacata ttgcaaaata
     4261 tgtggcctgc cagctcttcc tcctttgttt tcttttggct atctaacttc gagtatgggg
     4321 tataccgaac cagatatagc ccaagaaaaa atccttgaat tctttgctcg aatcgaacaa
     4381 gaagatatac cctgcgaagg catgtatttt tcttcaggct atgcaaaagc
//
LOCUS       DAOSOB010000092         4406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_19893_length_4406_cov_84.052172, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000092 DAOSOB010000000
VERSION     DAOSOB010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19893_length_4406_cov_84.052172"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1916
                     /locus_tag="PLS88_07995"
     CDS             138..1916
                     /locus_tag="PLS88_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOU07324.1"
                     /translation="MNTSIRKGVITRVSGPVVLARGLEDAGLYDVVKVGESGLIGEII
                     KLDGDKATIQIYEDNTLMRVGEPVECMHRPLSVTLGPGLIGSIYDGIQRPLPVLKRLS
                     GAFLKPGLAGEPLDTEKKWHFVPLLHEGDPIGQGIAYGRIQETSSIEHRLIFDARVPA
                     GIAAYVAPEGDYTVAETLVRSADGASYTSVSNWPVRKARPFREKQMTREPLVTGLRVI
                     DVLFPIAKGGCAAVPGGFGTGKTMTQHAIAKWCDADIIVYIGCGERGNEMTEVLTEFP
                     SLVDPRTGRSLMERTVLIANTSNMPVAAREVSIYTGITIAEYYRDMGYHVAVMADSTS
                     RWAEALRELSGRLEEMPAEEGFPAYLPTRLAEFYERGGMVATLEQKMGSVTIIGAVSP
                     PGGDFSEPVTQHTRRFIRCFWALDRDLANARHYPAISWNDSYSEYTDDLIAHWDAIDP
                     SWDRLRIKAMDILKKERRLSDIVRLIGPDALPDEQRLILLTADMIKNGFLQQSSFDKV
                     DMYCAPQKQTLLLQCILAFHELAESAIKNGAPLPKVSALPIREKIVRLKSSLENDKLE
                     EGRVVIQEIQAAFEQLGLTVQGAVLA"
     gene            1913..3367
                     /locus_tag="PLS88_08000"
     CDS             1913..3367
                     /locus_tag="PLS88_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOU07325.1"
                     /translation="MRGLAYHGIERADGPIVIAERRENIGFSELVRVKDRQGEYRLGR
                     VVELSEKAVAIQLFAENTGISIDDAWVEYLEKPLTFRVGEGIVGRIFNGLGEPIDGYP
                     PIMSSDLRDINGLPINPSARVYPRDFIQTGISAIDGMNTLIRGQKLPIFSGNGLPHNR
                     LAAQIVRQAKVLSGESQFAIVFAGMGIKYDVARFFIDSFESSGVLARVVMFLSLADSP
                     SIERLVTPRTALTAAEYLAYEKNMHVLVVMTDMTNYCEALREVSAARNEVPSRKGYPG
                     YLYSDLASIYERAGRIEGSEGSITQLPILSMPNDDISHPIPDLTGYITEGQIVLERDL
                     SQRGVYPPIAGLPSLSRLMKDGIGEGMTREDHRELAAQLFSAYAYVHTVRDLAAIIGE
                     EELSDRDKIFLNFGDHFESEFLAQGEFENRSIEQTLDLGWKMLSLLPESELVRIPPQI
                     LAKYLPHNDSDNRNDQNDQEKSHEVAILKGLKEN"
     gene            3371..3994
                     /locus_tag="PLS88_08005"
     CDS             3371..3994
                     /locus_tag="PLS88_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOU07326.1"
                     /translation="MAEKMPAPTKTNLIKEKRNLQLALQGYDLLEKKREILVIELMKR
                     MDSLELLEREIADMTEKAYAALRKMLLLVGRERALEISSLPVREITLSASHINVSGMR
                     LPSLDVHASNPTLNYSFMNSFAICDETVIEFTELLDKLSTAAGMRSVVWRLAREVRKT
                     QRRVNALDKMVIPRSKSIVKYIDASLDERERESLFAVKMLKRRQIGQ"
     gene            4000..>4406
                     /locus_tag="PLS88_08010"
     CDS             4000..>4406
                     /locus_tag="PLS88_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU07327.1"
                     /translation="MKKMLGTILVAVNGSDASICAFKYALALAKAFGSKIVACYVVDT
                     ATIRQLALSRIFVPEESEEYERNLENSGQRYLHFCAELAHQKQLDIETSIRRGSVSGE
                     IVKYSMEIDADIIVLGGNPVDVIYRDAIADSNH"
BASE COUNT         1137 a          990 c         1195 g         1084 t
ORIGIN      
        1 cttttggaat ggctcttaga tgaaaagaga ggcgagcttg cagcggcgct atttgggagt
       61 tctgattgaa ttcagtggta gatgcaagaa aatagatgcg gagaaacgag tgcgagaaaa
      121 atagggcaag aaagaatatg aatacaagca tccgaaaagg ggtaattacg agagtttcgg
      181 ggcctgttgt ccttgctcga ggcctggagg acgcggggct ctacgatgta gtaaaggtcg
      241 gagaatcggg gctaattggg gagatcataa agctcgatgg agacaaggcc acgattcaga
      301 tctacgaaga taacacgctt atgcgcgtgg gagagccagt ggagtgcatg catagacccc
      361 tctccgtgac gctcggacct ggccttatag gttcgatcta cgatggaatt cagaggcctc
      421 tgccagtgct aaaaagactt tcgggtgctt ttcttaagcc tggacttgct ggcgaaccgc
      481 tcgatacaga gaaaaaatgg cattttgtgc cgcttctcca tgagggcgat cccataggtc
      541 agggtattgc ctatggaagg attcaagaga cttcttccat tgagcatagg ctaatattcg
      601 atgcgagagt tcctgcggga attgcggctt atgtggctcc agagggcgat tacacagttg
      661 cagaaacgct tgtaaggtcg gccgatggtg ctagttatac ttcggtttcc aattggcctg
      721 tgcgtaaggc gcgtccgttt agggagaagc agatgacgcg cgagccgctt gtaacgggct
      781 tgcgggtcat agatgtgctc tttccaattg cgaagggcgg gtgtgcggcg gttccgggtg
      841 gttttggaac gggcaagacg atgacgcagc atgctatcgc taagtggtgc gacgcggaca
      901 taattgtgta cattgggtgc ggcgagcgcg gcaacgagat gaccgaagtc ctgaccgagt
      961 ttcctagcct tgtcgatcct cggactggtc gctcgctcat ggaacgcacc gtgctcatcg
     1021 ccaatacatc gaatatgccc gttgcggcgc gtgaagtttc gatttataca ggaattacaa
     1081 tagcagaata ctatcgcgat atgggatacc acgttgcggt catggcggat tcaacatctc
     1141 gctgggccga agcgctgagg gagctgtcag gtcggcttga ggaaatgcct gccgaagaag
     1201 gttttcctgc ctatcttcct acgcggctag ccgagttcta tgagcgtggc ggcatggtcg
     1261 ccacattaga gcaaaaaatg ggctcggtga cgatcatcgg agcggtttcc cctcccggag
     1321 gtgacttctc tgagccagtt acacagcata cgaggcgatt tattcgttgc ttttgggcgc
     1381 tcgatcgtga ccttgcaaat gcgcgtcact atccagcgat ttcttggaac gattcctaca
     1441 gcgagtacac cgatgatttg atagctcact gggatgcaat cgatccatct tgggataggc
     1501 ttagaataaa ggcaatggat attctcaaaa aagagcgaag gctttcggat atcgtgcgcc
     1561 tcattggccc agatgcgctg ccggatgagc agcgactcat actccttacc gcagacatga
     1621 tcaaaaatgg atttctacaa cagagcagtt tcgacaaggt cgatatgtat tgtgctcctc
     1681 aaaagcaaac tcttttgctt caatgtattc ttgctttcca tgagcttgcg gagagtgcaa
     1741 tcaagaatgg tgctcctctt ccaaaggtat ccgcgcttcc gatacgggag aaaatcgttc
     1801 gcctcaagag ttcgctagag aatgataaat tagaagaagg cagagtcgta atccaggaaa
     1861 ttcaagctgc cttcgaacag cttggcttaa ctgtacaagg agcggttcta gcatgagagg
     1921 tcttgcatac catggaatag agcgagccga tggtccaatt gtaattgccg aacgcaggga
     1981 gaacattggc ttttccgagc ttgtgagggt caaagatcgg cagggagaat atcggcttgg
     2041 acgggttgtg gagttatctg aaaaagcggt agccattcag ctttttgcgg agaacaccgg
     2101 catatctata gatgacgcct gggtcgaata tcttgaaaag cccctcacct tccgtgtcgg
     2161 cgaaggcatt gtcggcagga ttttcaatgg ccttggcgag cccatagatg gttatccacc
     2221 tataatgtcc tcggacctca gggatataaa cggccttccc atcaatccga gcgctcgcgt
     2281 gtaccccagg gatttcattc agaccggaat ttcagcgata gatggcatga ataccctcat
     2341 tcgaggtcag aaattgccga ttttttctgg aaacggtctt cctcacaata ggttagccgc
     2401 ccagatagtg aggcaggcaa aagtactcag cggagaaagt cagtttgcta tagtatttgc
     2461 cggtatggga ataaaatacg atgttgctag attctttata gattctttcg aaagttccgg
     2521 tgtcctagct cgagtggtga tgttcctatc gctggcagac agtccatcga tagagcgcct
     2581 tgtcacgccg cgcacggcgt tgaccgccgc tgaatatctc gcatacgaga agaacatgca
     2641 tgttctcgta gtcatgaccg acatgacaaa ctattgtgag gctcttcgtg aagtctcagc
     2701 ggcacgcaac gaggtgcctt caagaaaagg ctatccaggc tatctctatt cagacctagc
     2761 ttcgatctac gagagggcgg gaagaatcga aggcagcgag ggatccataa ctcagcttcc
     2821 tattctttcg atgccgaatg acgacatttc gcaccctatt ccagacctga caggctatat
     2881 aaccgaaggt cagatcgtgc tagagcgaga cctttcgcag cgcggagtgt atccgcccat
     2941 tgcagggctt cccagccttt cgcgcctaat gaaggatggc ataggggagg gcatgacccg
     3001 agaagatcac cgcgagcttg ctgcccagct tttttctgcc tatgcctatg tccatactgt
     3061 gcgagacctt gcagccataa taggagaaga ggagctttcg gacagggaca aaatattcct
     3121 caattttggt gatcattttg agagcgaatt cttagcccaa ggcgaattcg aaaatcgcag
     3181 catagagcag acgctagatc tgggttggaa gatgctctcg cttcttccag aatcggagct
     3241 tgttcgcatt ccgcctcaga ttctagcaaa atatctgccc cataatgata gcgataatcg
     3301 gaatgatcag aatgatcagg aaaaatccca cgaagtggcc attctaaaag ggctaaagga
     3361 gaactaataa atggccgaaa aaatgcctgc gcctaccaag acgaatctca tcaaggagaa
     3421 acgcaatctg cagcttgcgc tacagggtta cgatcttctt gagaaaaaga gggagattct
     3481 cgtcatcgag ctgatgaagc gcatggatag tcttgagctc ctagagcggg aaatcgcaga
     3541 tatgacagaa aaagcctatg cagctttgcg aaagatgctt cttttggtcg gccgagagag
     3601 agcgcttgaa atttcatctt tgccagtgcg cgaaatcact cttagcgcat ctcatatcaa
     3661 tgtaagcggg atgcggcttc cttcgctcga tgtacatgct agtaatccta ccttgaatta
     3721 ttcttttatg aattcatttg ccatatgtga cgaaaccgtg atagagttta ctgagcttct
     3781 tgacaagctt tcgacagcag caggtatgag gtcggttgta tggcgccttg cgcgagaagt
     3841 ccgcaagact cagcgcagag tgaacgcatt ggacaagatg gtgatacctc gatcgaaatc
     3901 gatcgtgaaa tatatcgatg cttctctaga tgagcgggag cgcgaatcgc tgtttgcagt
     3961 aaagatgctt aagaggcggc aaataggaca gtaagaatca tgaagaaaat gctagggacc
     4021 atattggtag cggtcaatgg ttcagatgct tccatctgtg ctttcaaata tgctttggcg
     4081 ctcgcaaaag cctttggctc gaagattgtt gcctgctatg tagtcgacac ggcgaccatt
     4141 cgtcagcttg cactttccag aatcttcgta cctgaggaga gcgaagaata cgagcgcaat
     4201 ctcgagaatt cgggtcagcg ctatctccat ttttgtgcag agcttgctca tcagaaacag
     4261 ctagatatag agacatccat ccgccgaggc tccgtttctg gagaaatagt caaatattcg
     4321 atggagatcg atgctgatat aatagtgcta ggtggtaatc cggtcgatgt gatctatcgc
     4381 gatgcgattg ccgattccaa tcacga
//
LOCUS       DAOSOB010000093         4262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_20777_length_4262_cov_82.482767, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000093 DAOSOB010000000
VERSION     DAOSOB010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20777_length_4262_cov_82.482767"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1466)
                     /gene="mtaB"
                     /locus_tag="PLS88_08015"
     CDS             complement(48..1466)
                     /gene="mtaB"
                     /locus_tag="PLS88_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOU07328.1"
                     /translation="MAVLRVAFQTLGCKLNQLETESIADKFLAVGAEIVPFHDVADLY
                     IVNTCTVTSKAEQKARHDIRSVITKLRNTIVIATGCYAQMDSNELSSIDERIVVISGD
                     MKASLLSLPQWLLDNWQGHGELLHAVLEWRDEVLAAATETAKEAVSSLSRFEFHPDRF
                     LMHSRPALKIQDGCNNRCAYCRVCLARGPSISLPIDEALSRVICLEKAGKAEVVLTGV
                     NLAQYRSGGLDFPGLLASMIRATSAIRFRISSYEPQNIDEAFLSIFAHPRVRPHLHLS
                     VQSGCTETLRRMGRPYCAEDLRFAVQNLRSVRCDPFLAADIIAGFPGETESEFENTIE
                     LLSQLDFAWIHAFPFSLRPDTRAASMKPRIPERIAGERVKRLLGLARQGKEAYVNRWI
                     GKCVEMVIEHERRGIEDSQARLIIGTTENYLKAMIEESESVAHKIIPYRPGGSVKVVL
                     NSMSESALPDGGVEVLAKIAEA"
     gene            complement(1485..2165)
                     /locus_tag="PLS88_08020"
     CDS             complement(1485..2165)
                     /locus_tag="PLS88_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU07329.1"
                     /translation="MKCPAHKIRRIALIGMFLFSIQISGLFGQSSSSPISVPSGMTGS
                     DLLRMSQEGKHNDVALLAPASIKKNPQDVDAYIGLAWSLNALKEYVKAKDIAQQGYAR
                     FKDPRLAQALGEAQYYLGENEAALANLQEYLARFPEGSKAALTFYLCGELFVRMDKFN
                     HADIAFSTALQYNPSNARWWVRLGWVREKTARYIPALKAYEQAINLSPNLTEALNGKS
                     RMLAKIED"
     gene            complement(2162..2599)
                     /locus_tag="PLS88_08025"
     CDS             complement(2162..2599)
                     /locus_tag="PLS88_08025"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU07330.1"
                     /translation="MQSIDNYNLYKHPLLLAEIRLILYHIGKMAEEVKLSVVDEEEVD
                     TVIGSEIEFEGDIESSKSLMIKGKVSGNINCSAELYITEQAEVRSTIHAATVVIRGKV
                     YGDISADSLIAVLDWGHVEGRLVAPDIYLSPNCVFKGTTVIKS"
     gene            complement(2568..3443)
                     /gene="rsmA"
                     /locus_tag="PLS88_08030"
     CDS             complement(2568..3443)
                     /gene="rsmA"
                     /locus_tag="PLS88_08030"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOU07331.1"
                     /translation="MSQDKLNYDSPASIQAFLEQNGLAVSKRLGQNFLIDRSVRSRIV
                     KALEVEEGMQVWEIGPGIGAMTILLLEEKVRLTAFEIDYGFVRVLKGLFSEYENFCIV
                     KGDVLKTLCSQKDLPARIFGNLPYNSAFAIISDILEMEILPQHMVFTLQKEAAHRMVA
                     GPGTKDYSSLSVLCTSVCDVRILFDIGSSAFWPRPRVTSSAVQLIPKKKSMSALERKD
                     FSDFVRAAFSSRRKTLRNTLRFWIKQRSPFLDEAKIDTQLQGILEELGIESSIRAEAL
                     APDELFAIYRQLQSI"
     gene            3710..4105
                     /locus_tag="PLS88_08035"
     CDS             3710..4105
                     /locus_tag="PLS88_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07332.1"
                     /translation="MSKEARILPIGARPASKMGKAAPDARIQARTECKTSNTGAVIRP
                     ITGKGIAKSAPAKAIHIKTSMIGAAAVAAKIPAGIKVPKVKSDTGAVPHTAAKDAENA
                     LASKSGIQCLKIIKNRPLNAISPAIALKE"
BASE COUNT         1176 a         1072 c          939 g         1075 t
ORIGIN      
        1 gaaggaaaaa ctatagcacc aaaataggcc tgcgcttaca agaaccccta tgcttctgca
       61 attttcgcta gcacctctac acccccatcc ggcaatgcac tttcgctcat gctattcaaa
      121 acgaccttaa ccgaaccccc tgggcgatat ggtattatct tatgggccac gctttccgat
      181 tcctcaatca tagctttgag ataattttcg gttgtaccaa taattaacct agcttgggaa
      241 tcctctattc cgcgtctttc atgctcgatt accatctcga cgcattttcc aatccagcga
      301 ttcacataag cctctttccc ttggcgagca aggccaagga gtcgcttcac cctctcccct
      361 gcgattcttt cagggattcg cggtttcatg cttgcggcac gcgtatcggg ccttaggcta
      421 aaaggaaaag catgtatcca agcaaaatcg agctgtgata acagttcaat ggtgttctca
      481 aactcggact ccgtctctcc tggaaagcct gctattatgt ccgctgcgag gaaagggtcg
      541 catcggactg aacgcaaatt ctgtacagcg aacctcaaat cttcggcgca gtatggtctt
      601 cccatgcgcc ttagtgtctc ggtgcagccc gattgcacgg aaagatgcaa gtgagggcgt
      661 actcgcggat gagcaaaaat cgaaagaaag gcttcatcga tgttctgtgg ctcataactc
      721 gatatgcgaa atctgatagc gctcgtagct cttatcatcg atgcaagaag acctgggaaa
      781 tcgagcccgc cagatcgata ctgcgcaagg ttgacacctg taagaacaac ctcggccttg
      841 ccggcttttt ctagacagat cacacgagac aatgcctcat caatcggcaa ggatattgaa
      901 gggcccctag caaggcacac acggcaatag gcgcagcgat tgttgcagcc atcctgaatc
      961 ttgagcgcag gccgagaatg catgagaaat ctatctggat ggaattcaaa tcgactgagc
     1021 gatgacactg cttctttagc cgtttctgtg gctgcagcca gaacttcgtc gcgccattcg
     1081 agtacagcat ggagaagttc accatggcct tgccaattat ccaaaagcca ttgaggaagc
     1141 gaaagaagtg aagctttcat atctcccgag ataacgacga tgcgttcatc tatcgaagag
     1201 agctcattgg agtccatctg agcatagcaa cctgtggcaa tgactatagt gttccgaagc
     1261 ttggttataa cggagcgaat atcatgccgc gccttttgtt cggccttgga tgtcactgta
     1321 caagtattca cgatatagag atcagcaaca tcatgaaatg ggacaatctc tgcacccaca
     1381 gccagaaatt tgtctgcaat ggattcggtc tcaagctgat tgagtttgca gcctaatgtt
     1441 tgaaatgcta cccgcaaaac tgccattcga gaatttctcc gccctcaatc ctcaatctta
     1501 gctagcatac gcgattttcc attgagggct tccgttaggt tgggacttag atttatagct
     1561 tgctcgtagg ctttgagcgc cggaatatat cgcgctgttt tttcccttac ccacccaagg
     1621 cgcacccacc atcgagcatt gcttggattg tactgtagag ccgtgctgaa tgctatgtcg
     1681 gcatgattga atttgtccat acgcacaaaa agctcaccgc ataaatagaa tgtaagggcg
     1741 gccttgcttc cttctggaaa tcttgccaga tactcctgca gattagctaa tgccgcttcg
     1801 ttttctccaa ggtaatattg ggcttcgcct aatgcttggg caaggcgcgg atccttgaac
     1861 ctagcatagc cttgctgtgc tatatccttg gcttttacat attcctttaa ggcattgaga
     1921 ctccatgcaa ggcctatgta tgcatcgaca tcttgaggat tcttcttaat tgacgctggg
     1981 gcaagaagag cgacatcatt atgctttcct tcttggctca tgcgaagcag atccgagcct
     2041 gtcatgccag atgggacact tatcggagaa gatgacgact gaccaaatag cccagaaatc
     2101 tgtatagaga aaagaaacat accaataagg gctattcgcc taattttatg agctgggcat
     2161 ttcatgattt aataaccgtc gtccctttaa aaacacaatt tggcgaaaga tagatgtctg
     2221 gagcaaccaa gcgtccttct acatgccccc aatcgagcac ggcaataagg gaatcagcac
     2281 tgatatcgcc atacactttc ccacgaatca caactgttgc cgcatgaatt gtcgatcgca
     2341 cttcagcttg ctcggtgata tatagttcgg cactgcagtt tatatttccg ctcactttgc
     2401 cctttatcat gagcgatttc gaggattcga tatctccctc gaattctatc tcagagccga
     2461 ttacggtatc tacttcttcc tcatcgacca cgctaagctt tacttcttca gccatcttgc
     2521 caatatgata gagaattagc ctaatctcag caagaagcag agggtgctta tatagattgt
     2581 aattgtctat agattgcaaa aagctcgtct ggtgcaagag cttcggcccg gatagaagat
     2641 tcaattccaa gctcctcgag gatcccttgc aattgtgtat cgattttcgc ttcgtctaaa
     2701 aaaggagagc gttgcttaat ccaaaaacgc aaggtgtttc gaagcgtttt tcttcgagag
     2761 gaaaaagcag cccgcacaaa atcggaaaaa tccttgcgct ctaaggcaga catcgatttt
     2821 ttcttcggaa taagctgaac ggcagagctt gtaacccttg gccttggcca aaatgcgctc
     2881 gagccaatat cgaataggat gcgtacatcg cacaccgaag tgcagagcac actcagtgag
     2941 gaataatcct ttgtccccgg accggctacc attcggtggg cggcctcttt ttgaagagta
     3001 aaaaccatat gctgcggcaa aatttccatc tcaagaatat ccgaaatgat agcgaatgca
     3061 gaattgtatg gtagatttcc aaatatgcgc gcaggtaagt ccttttgcga acacaatgtt
     3121 tttagcacat ccccttttac gatacaaaag ttttcatatt ctgaaaaaag gcccttgagt
     3181 accctcacaa atccataatc gatttcgaag gctgtaagtc gtactttttc ttcgagaaga
     3241 aggatagtca ttgcgccgat accagggcca atctcccaaa cctgcatgcc ctcttctacc
     3301 tctagtgctt ttacaatgcg gctgcgcacc gagcggtcta ttagaaagtt ttgccccaag
     3361 cgcttgctga cggcaagccc attctgctca agaaaagcct gaatcgaagc tggagaatcg
     3421 taattcagtt tgtcctggct catagactta tgcctacttc aaatattggg cgccttatct
     3481 tattgttctt tttctggaga atgccagcct aatgaacgtg ctttcgacat cattttgacg
     3541 gacccgagcg taatgagcat gctttcggtc ttattccggc ggatctaacc taataagcat
     3601 tctctttata tcaacaaagg acgcgcaccg ataaaactct tcagttttct gcctcgattt
     3661 cctccagctc gcccatggaa tagcataaat cagagcgata agcgaaacaa tgagcaaaga
     3721 ggcaaggatc ctgcctattg gcgcaagacc tgcttcgaag atgggaaaag ccgcgcccga
     3781 tgcaagaatc caggcaagaa cagaatgcaa aacctccaat acaggtgcgg ttatccgccc
     3841 tataacaggt aaaggaattg ccaaaagtgc accagccaaa gcgatccaca taaaaacaag
     3901 catgatcggt gcggcagccg tcgccgcaaa gataccagca ggaataaagg ttccaaaggt
     3961 caaaagcgat accggagcag ttccgcatac agcagctaaa gatgccgaaa atgccttagc
     4021 tagcaaatct ggcatccaat gcctcaaaat aatcaagaat cggccactaa atgccataag
     4081 cccagccata gcgctgaagg aatagatact cgaaagggaa tttatcgaac gcgggctaaa
     4141 tagcgctaac aaaatcgatg ccagcgaaag aagcgcaaag cccgattgcg gtctatccaa
     4201 ttccctcgca atctccgcgc agctcagcat aaagacagat ctcaaaagcg agggcccagg
     4261 cc
//
LOCUS       DAOSOB010000094         4250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_20854_length_4250_cov_64.351371, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000094 DAOSOB010000000
VERSION     DAOSOB010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20854_length_4250_cov_64.351371"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..777
                     /locus_tag="PLS88_08040"
     CDS             43..777
                     /locus_tag="PLS88_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOU07333.1"
                     /translation="MSNNRKIIADNVAIIKERICHAAEKAGRSIDDIELMAVTKFQPI
                     DVIQQAYLAGIRLFGENRVQEALAKYPLLEPDLEGAFLHMIGTVQKNKINKALTIFDA
                     IQGIDNIETLEAILARIKASKKPIKLYLELHTGEISKAGFPNVDELLRGCEIYAEFLQ
                     SDHQGARKASLCGLMTMAPFTQDVPLIRRSFKMLSAARDKVKSSFDFPEFDELSMGMS
                     NDFEIAIEEGSTLVRIGTAIFGERKA"
     gene            778..1209
                     /locus_tag="PLS88_08045"
     CDS             778..1209
                     /locus_tag="PLS88_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07334.1"
                     /translation="MNKRCFILLIVFICGSLFVYCLPVHAQEENSDSEIVAEEKQNAQ
                     EMLEVPKVTLPSFSYSETKEGAVKRGIVISLGLFPFSYFYTNFAFDLVRFISHDFDTA
                     YAPWPFNTQYSAALTNSEVWTRIGIASGASILLGFLSVIIE"
     gene            1299..1859
                     /locus_tag="PLS88_08050"
     CDS             1299..1859
                     /locus_tag="PLS88_08050"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOU07335.1"
                     /translation="MANGSDVRGDYQKFNLDDAIRKIPDFPKPGILFYDITSILANPK
                     AFQYCIDSMLELYGETEFDAVAAIESRGFVFAAPFCYRRNIPLLLVRKKGKLPGKTIS
                     CSYELEYGTAVLEMHIEDIKAGDRFLIIDDLIATGGTINATCEMIKRCGAVPVRAFSV
                     IGLPSLNYEEKVIDVGIDTLIEYFGE"
     gene            1936..3603
                     /gene="gpmI"
                     /locus_tag="PLS88_08055"
     CDS             1936..3603
                     /gene="gpmI"
                     /locus_tag="PLS88_08055"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOU07336.1"
                     /translation="MLEPLKKNPNWKGREGSLVLVIMDGVGYGAYKEGDAVLAADMEH
                     FRELEASCPTTHLKAHGTAVGLPSDDDMGNSEVGHNAIGCGRVFAQGARLVNISIESG
                     AIFEGRVWKELIANIKATGGTLHFIGLFSDGNVHSHIDHLKAMLVQAQKEGVKRARIH
                     ILLDGRDVGSQSALEYVLPFEDFLEEFRNQGADYRIASGGGRQYITMDRYGANWQIVK
                     RGWDCHVRGIGRQFNSAEEAILTYRKEIPDIIDQDIPEFVIAKNGEPIGTIEDGDSVI
                     FFNFRGDRALEITAAFEQDNFDKFNRVRRPKVYYAGMMQYDGDLKVPTNFLVSPPAID
                     RTIGEYLASTSLKTLAISETQKYGHVTYFFNGNRSGKFSEELEDYIEIPSDRVPFEQR
                     PWMKCAEIADYVVSAIKKGLYSFIRLNFPNGDMVGHTGVFQAVVCGLEGMDIQLGRIR
                     EAVEKAGGILVITADHGNSDDMYEHDKKTGKVILDLNGKPKAKTSHSLNPVPCVIYDP
                     QYQGEYKRELRNGLGISSLAATCIELLGYVPPEDYDPSVLDMAMKQE"
     gene            3611..4174
                     /gene="cyaB"
                     /locus_tag="PLS88_08060"
     CDS             3611..4174
                     /gene="cyaB"
                     /locus_tag="PLS88_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOU07337.1"
                     /translation="MMQTPEYAHNMEIELKARIQRRDEVESRLSSFMHYIGPIDKLDE
                     YWEVSNASSENSSGFRFRVRHELDRITITFKEKTFDGNVEVNHEFEFRIEGEPAFKAF
                     IEKMHAHLIYQKQKKGSRWEDGTGLIAEVVEVSSLGLFLEVEYISNSLATCEQQEAKS
                     RLYKVIDLCDIPRSALEPRPYSQLLGY"
BASE COUNT         1270 a          810 c         1036 g         1134 t
ORIGIN      
        1 gaaagcttgc tcagatttct gttcgctgat aggattttgg atatgtcgaa taatcgaaag
       61 atcattgcag ataatgtggc tattataaaa gaacgaattt gtcacgcagc agagaaggct
      121 ggaagatcta tcgatgatat tgaactcatg gctgtgacta aattccagcc tattgatgtg
      181 attcaacaag cctacttggc aggcattagg cttttcgggg aaaatagagt acaggaagca
      241 ttggcgaaat accctctttt agagccagat ctggaaggcg cttttttgca tatgattggt
      301 acggttcaga agaacaagat caataaagct ttgacaatct tcgatgcaat tcaggggata
      361 gacaatatag aaacgctaga ggctatcctt gcaagaatca aggcctctaa aaaacctata
      421 aaactctatt tagagttgca tacgggagag atttcgaaag ctggcttccc taatgtcgat
      481 gagctcttac gaggatgcga gatttatgcg gaatttctcc aatcagatca tcagggagcg
      541 agaaaagcct cgctctgcgg tctaatgacc atggcacctt ttacgcagga tgtcccattg
      601 atccgtagat ctttcaaaat gctctccgct gcaagagaca aagtcaagag cagctttgat
      661 tttccagaat ttgacgagct ttctatgggt atgagtaatg attttgaaat cgcaatagag
      721 gaaggttcta cacttgtaag gataggcact gctatttttg gcgagaggaa ggcataaatg
      781 aacaagcgtt gctttatttt gctcattgtt tttatttgtg gaagcctttt tgtttattgt
      841 cttccagtac acgctcagga agaaaattcg gactccgaaa tcgtcgcaga agaaaaacag
      901 aacgctcaag aaatgcttga agtgcccaaa gtgactctac caagtttttc ttatagtgag
      961 actaaagaag gcgcagttaa gcgtggcatt gtgatttctt taggactttt tccctttagc
     1021 tatttctata ctaattttgc tttcgatttg gtccgtttta tatcacatga tttcgacacg
     1081 gcctacgcgc cttggccatt caatacccag tatagtgcag ctttaacaaa cagcgaggta
     1141 tggacgagga ttggtatcgc ttcaggagca agtatcttgc tcggtttctt gagcgttatc
     1201 atagagtgat atacaaaaag cactgtattc tgtatactgc agcgcaacct ttatataagg
     1261 tcgcgccata atttccattg gtaagagagg caaaaaatat ggcgaacggc agcgatgtca
     1321 gaggcgatta tcaaaaattt aacctcgatg atgcaatccg taagattcca gatttcccta
     1381 aaccaggtat attgttttat gacatcacga gcattcttgc taatcctaaa gcatttcaat
     1441 attgcatcga ttcaatgctt gaactatacg gagagacgga attcgatgca gtggctgcca
     1501 tagaatcgcg aggttttgta tttgcagcgc ctttttgcta taggcggaat atccctttgc
     1561 ttctagttag gaaaaaaggc aaattgccgg gaaagaccat atcctgctct tatgagttgg
     1621 aatatggaac tgcagtgcta gaaatgcata tcgaggacat caaagcaggt gaccgctttc
     1681 tgataatcga tgaccttatc gctacaggag gcactattaa cgcgacctgc gaaatgataa
     1741 agcgctgtgg agcagtacct gttcgcgcat tctcggtaat aggcttgcca tctttgaatt
     1801 atgaagagaa agtgatagat gtcggcatag atacactcat cgagtacttt ggggagtaga
     1861 aatataggaa gcctattggg caaagcagct ccgaactcat ttgaagctag ccaattatag
     1921 aatagggagc aatttatgct tgaaccatta aaaaaaaatc cgaattggaa aggtagggaa
     1981 gggtcgctag ttcttgtgat aatggatggc gtcggctacg gcgcttacaa agaaggcgat
     2041 gcagtgcttg cagcagatat ggaacatttc agagagcttg aggctagctg ccccacaacg
     2101 catcttaaag cccatggaac tgccgtaggc cttccctccg atgatgatat gggaaactcg
     2161 gaagtcggtc acaatgctat aggttgtggc cgagtttttg cgcaaggtgc gcgacttgtc
     2221 aatatttcga ttgaatctgg ggcaatattt gaaggaagag tctggaagga actcattgcc
     2281 aatataaaag cgactggagg aactctgcac tttattggcc ttttctcaga tggaaatgtc
     2341 cattcgcaca tcgatcattt aaaagctatg ctagttcaag cccagaaaga aggtgtcaaa
     2401 cgagcgcgga tacatatctt gctcgatggc cgggatgttg gaagccagag tgctcttgaa
     2461 tatgtgcttc cttttgaaga ttttcttgag gaatttcgaa atcaaggagc ggattatcgc
     2521 attgcttctg gaggcggaag gcaatatata acgatggacc gttatggtgc caattggcag
     2581 atagtgaagc gcggttggga ttgccatgtc agaggtatcg gaaggcaatt caacagtgca
     2641 gaagaggcaa tcctgactta tcgaaaggag attccagata ttatcgatca ggatattcct
     2701 gaattcgtaa ttgctaaaaa tggggaaccc atcggcacaa tcgaagatgg ggattcggta
     2761 atatttttca atttcagagg tgacagggcg ctcgagatta cggcagcctt cgaacaggac
     2821 aatttcgaca aattcaatag agtcagacga ccaaaggtat attatgcagg catgatgcag
     2881 tacgatggag acctaaaagt tcctaccaat ttcttggtgt caccgcccgc aatagataga
     2941 acgatcgggg aatacttggc ttccacaagc ctcaagacac ttgctatttc ggagacgcag
     3001 aaatatggcc atgtaactta ttttttcaat ggcaataggt ctggaaaatt ttcggaagaa
     3061 ctcgaagact atatcgaaat accaagcgat cgcgtgccat tcgagcagag accatggatg
     3121 aaatgcgctg aaatagcaga ttatgtcgta agtgctatca aaaaaggttt atatagcttt
     3181 attcgcctca attttcccaa tggcgatatg gtaggccata caggagtctt ccaagctgta
     3241 gtatgtggct tggaaggaat ggatatacaa ctaggcagaa ttcgagaggc ggtagagaaa
     3301 gcggggggca ttcttgttat tacggctgat catgggaatt ccgatgatat gtacgagcac
     3361 gacaagaaga cagggaaagt tattctggat ctcaatggca agccaaaagc aaagacaagt
     3421 cattctctca atcctgttcc ctgtgtgatc tacgacccgc aatatcaggg tgagtacaaa
     3481 agagaattga gaaatggtct gggaatctct agcttggctg caacatgcat cgagctttta
     3541 ggctacgttc ccccagagga ttatgatcca tcagtgctgg atatggctat gaagcaagag
     3601 taatagggca atgatgcaga ctccagaata tgcccacaac atggagatag aacttaaggc
     3661 taggattcag cgaagagatg aggttgaatc tcgcctttcc agttttatgc actatatagg
     3721 acccatcgat aagctagacg agtattggga agtttcgaat gcaagttcgg agaattctag
     3781 cggttttcgg ttccgcgttc gtcatgaatt ggatagaatt actattacat ttaaagagaa
     3841 aaccttcgat ggaaatgtgg aagtcaacca tgaattcgag tttcgcatcg aaggcgaacc
     3901 agctttcaag gccttcatag agaaaatgca tgcacatctg atctaccaaa agcaaaagaa
     3961 aggtagccgc tgggaagatg gaacaggctt gatagccgag gttgttgaag tatcctcgct
     4021 cggccttttt cttgaagtcg aatacattag caatagtctg gccacatgtg agcaacaaga
     4081 ggctaaatcg cgattatata aggtaatcga tctatgtgat atcccaagga gcgctctcga
     4141 gcctcgaccg tattcgcaat tgttaggata ctgaaatagg atactgaaag ccgaatcaat
     4201 aagaattatg ggcgctgacg gatttgaacc gccgacctac tgggtgtaaa
//
LOCUS       DAOSOB010000095         4022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_22495_length_4022_cov_81.468868, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000095 DAOSOB010000000
VERSION     DAOSOB010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22495_length_4022_cov_81.468868"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1541)
                     /locus_tag="PLS88_08065"
     CDS             complement(6..1541)
                     /locus_tag="PLS88_08065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOU07338.1"
                     /translation="MSNNKLDSKVLENWLWDAACIIRGPIDAPKYKDYILPLIFFKRL
                     SDVFEDELARLSQGYNSSREVVEALLVEDHKLVRFYLPKNARWEAVASHTTNVGEQLT
                     DAVRSIARENPKLQGVVDTVDFNATASGQRILADDKLKELIDVLGRYRLGLNDVDADI
                     LGRAYEYLLRKFAEGSGQSAGEFYTPGEVAILMSHILDPEPGDEVYDPCCGSGGLLIK
                     CFLRFKEKYNSEPSLEPLTCYGQEILPSTFALAKMNAFIHDMEAEIALGDTMVRPAFL
                     TPQGALKKFDIVTANPMWNQDFSQTVYENDAYNRFTFGYPPSSSADWGWIQHMYASLN
                     DKGKMAVVLDTGAVSRGSGNAGSNRERDIRKQFVENDLVEAVLLLPENLFYNATAPGI
                     IIVINKAKPAERKNTILLINASKLFQKGRPKNYLPDESIEQIADKYLGWNEEEGISKI
                     ITKEEATRNDYNLSPSRYVAQNGEDNTLDLEDAIVQLKEAEEERNQADEKLRTILKEM
                     GVW"
     gene            complement(1793..2962)
                     /locus_tag="PLS88_08070"
     CDS             complement(1793..2962)
                     /locus_tag="PLS88_08070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOU07339.1"
                     /translation="MDDFIFQNPTRIYFGKSHEANIGRIAAQYGKSVLLHYGGQSAER
                     SGLLPLIKKELTEAGLHFFELGGVQPNPRLSLVYKGIELCKSNHIDLVLAVGGGSAID
                     SAKAIAAGALYEGDVWDFYTGKAVPEKALPVGTVLTIPAAGSESSPGSVITKEEGQLK
                     RAVNADCLFPRFSVLNPERCYTLPPAQIANGATDIMCHLMERYFTNSRPVEFTDRLIE
                     GTLRTVIASAPRVLANPRSYNDWAELMWAGTIAHNNLLDTGREGDWASHDIEHELSGL
                     YDIAHGAGLAIVTPAWMKYTLKHDIARFAQYSVRVWGVEDNFFDPERVAREGIARLEA
                     FWRSIGQKVRLSEIGIDERHFTQMALKATDGEAHTVGHFVPLRAVDIEAIYRLAL"
     gene            complement(3285..3653)
                     /locus_tag="PLS88_08075"
     CDS             complement(3285..3653)
                     /locus_tag="PLS88_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU07340.1"
                     /translation="MKLFHPIEENGKSKGVETQDASSRFGNINAESAAKSEETKKVPG
                     VKVLGSGCARCQALEKAAKKALEELGMDTTIDHVTDFAQIASYGVMSTPALVVDGKVV
                     SYGKVLSKDEVKSLLQKVRG"
     gene            complement(3681..>4022)
                     /locus_tag="PLS88_08080"
     CDS             complement(3681..>4022)
                     /locus_tag="PLS88_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOU07341.1"
                     /translation="IGTVIHNWIPEAWISSVLGSRNPFGVVLATLIGVPIYADIFGTI
                     PVAEALLAKGAQLGTVLSFMMAVTTLSVPSMVMLSKAIKPKLLGLFVGICTLGIIIVG
                     YLFNAFHYLFI"
BASE COUNT          969 a         1017 c          947 g         1089 t
ORIGIN      
        1 tacttttacc atactcccat ttccttaagg attgttctca gtttttcgtc ggcctggttt
       61 cgttcttcct cagcttcctt gagctgcact atagcgtcct caaggtcaag ggtgttatcc
      121 tcgccgttct gggcaacata acggctgggg ctcaggttgt aatcgtttcg ggtagcttct
      181 tcctttgtaa taatcttgct gatgccttcc tcttcgttcc agccgagata tttgtcggca
      241 atctgttcaa tgctctcgtc gggcaggtaa ttctttggtc tgcctttttg aaaaagtttg
      301 gaggcattga tgagcaatat tgtattcttc cgttccgccg gtttagcctt gttgatgaca
      361 ataattatcc ctggggcagt ggcattgtag aagaggtttt cgggcaataa cagcacagcc
      421 tccacaaggt cgttctctac aaactgcttt cggatatcgc gctcgcggtt gctacccgca
      481 ttgccacttc cacgggacac agcgccagta tcgagcacga ctgccatctt gcctttgtca
      541 ttgagcgaag catacatatg ttgaatccag ccccagtctg cactgctgga aggcggataa
      601 ccgaaggtaa agcggttgta tgcgtcgttt tcgtacacag tctgcgaaaa atcctgattc
      661 cacatgggat tggccgtaac gatatcgaat tttttgagtg caccttgcgg tgtgagaaag
      721 gcgggccgca ccatggtatc gccaagcgcg atttctgcct ccatgtcgtg aataaaggcg
      781 ttcattttag ccaaggcgaa ggtggaaggc agtatttcct gcccatagca tgtaagcggt
      841 tcaagggaag gttcggagtt gtacttttct ttgaagcgca ggaaacattt gataagcagt
      901 ccgcccgaac cacagcaggg atcgtacact tcatcacccg gttcaggatc gaggatatga
      961 gacatcagga tggcaacctc gccgggagta tagaattcgc ccgcactctg gccggaacct
     1021 tcggcaaatt tccgcagcag gtattcgtac gctcttccca agatgtcggc gtcgacatcg
     1081 ttcaagccca gtcggtatct gcccagcaca tcgatgagct ctttgagctt atcgtcggcg
     1141 aggatccgct gccctgacgc ggtggcatta aagtctacgg tatcgaccac gccctgaagt
     1201 ttgggatttt cgcgtgcaat ggagcgaacg gcatcggtaa gctgttctcc cacattggtg
     1261 gtgtggctgg caacagcctc ccatcgtgca tttttgggta gatagaaacg cacgagcttg
     1321 tgatcctcta ccagcagcgc ttccacgact tctctggagg agttataacc ctgcgagagc
     1381 cgtgcaagct catcctcaaa tacatcagaa agccttttaa aaaagatgag tggcaagatg
     1441 tagtctttat atttcggcgc atcgatcggc ccacggatta tgcaggcagc atcccagagc
     1501 cagttttcca gaactttgct atcgagttta ttatttgaca tctctactcc attgttgcat
     1561 gcctttattt tacgccgcgc tcgcatataa tagaagagca gaacttggtt gcatttatgg
     1621 gctatcttct gcagtctttg atgacttctc tcttctgcca tgcggcaaag cggattcaag
     1681 gaatgcccaa acgcctattt ttctctttct ttcaagaaat aaataatcca gttcatcctg
     1741 caccatcctg tcatcatgtc taaacatcct gttcaggtat taggcgcagc aattacaacg
     1801 caagccgata gatggcttcg atatctactg cccgcagcgg cacgaaatgc cccactgtgt
     1861 gagcttctcc atcagtagcc ttgagcgcca tctgggtaaa atgcctttca tcgataccga
     1921 tctcggagag tctgactttt tggccaatcg agcgccagaa tgcctcgagg cgggcaattc
     1981 cttcgcgagc cacgcgctct gggtcgaaga aattgtcttc cacaccccag acacgcacag
     2041 aatattgggc aaaacgggca atatcgtgct tgagagtgta tttcatccat gcgggtgtga
     2101 cgatcgcaag tccagcccca tgcgcgatat cgtaaagtcc actcaattcg tgttcgatat
     2161 catgcgaagc ccaatctcct tcacggcctg tatccaatag attgttatgg gctatggttc
     2221 ctgcccacat gagctctgcc caatcattat aggacctggg atttgcaagt acgcgaggtg
     2281 cagaggcaat gactgtccga agtgttcctt ctatgagtct atcggtgaac tctactggcc
     2341 tagaattggt gaagtaccgc tccataagat ggcacattat gtcagttgcg ccattggcga
     2401 tctgggcagg ggggagtgta tagcagcgtt cgggattgag caccgaaaag cgagggaaaa
     2461 ggcaatcggc attgacggca cgcttgagct gaccttcttc cttggttatt accgaaccgg
     2521 ggctagattc gctgccagca gcagggatgg tgagcacggt tcctactggg agcgcttttt
     2581 caggaactgc cttgcccgta tagaaatccc agacatcgcc ttcatagagc gcacccgctg
     2641 ctatagcttt ggcagaatcg atggcgctac ctcctcctac agcaagcaca agatcgatat
     2701 ggttgctctt gcatagctcg atgcccttgt acacaaggct caggcgcgga ttgggttgca
     2761 ctccgccgag ctcaaaaaag tgtaaacctg cttcagtgag ctcttttttt ataagtggca
     2821 agagccccga gcgttccgcg ctctggccac cataatgcaa gagcacagac tttccatatt
     2881 gagctgcgat acgaccaata ttcgcctcgt ggcttttccc aaaataaatc cttgtggggt
     2941 tttgaaatat aaaatcatcc atagattctg ctccttttgg tttgcctcta taatatatca
     3001 ctctcaagat gtaccataaa aacataaacc cattgggcag attttgaaag atgccaaaaa
     3061 gtcttgagag ggcctttgat aggatgatcg agatattttt gacgggatga tcgggatttt
     3121 gcaggatgaa caggattttc ttataaggta aagaataaag tcctcatttg gccttctcgc
     3181 ggcgagaata atatcctgtg tatccttatc ttatcctgtc atcatgtcaa aatttgctaa
     3241 cagagtccaa gggcttgcaa agagcagaag cgaacttatc cgccttatcc gcgaactttc
     3301 tggagcaagc tcttcacttc gtccttgctg agcactttgc cataagaaac aactttacca
     3361 tcgactacga gcgcaggggt gctcatgaca ccgtacgatg caatctgtgc aaaatcagtc
     3421 acgtgatcga tggtcgtatc catgccaagc tcttcaagcg ccttttttgc tgctttctcc
     3481 agcgcctgac atcgcgcaca accgcttccc aagactttta ctcctggcac ttttttggtt
     3541 tcttccgact ttgccgccga ttctgcattg atgttgccga agcgggagga agcgtcttgt
     3601 gtttcgaccc ctttgctttt tccattttct tcaattggat ggaacagttt catgccaact
     3661 ctcctttatg taactagtat ttatataaat aaataatgga aagcattgaa taaataacca
     3721 acgataatga ttcccagcgt acaaatcccc acaaaaagac caagcaattt cggtttgata
     3781 gccttgctca acatcaccat cgagggaacg ctgagcgtgg tcaccgccat catgaacgaa
     3841 agtacggtac caagctgtgc accttttgca agcagcgctt ctgctacagg aatcgttcca
     3901 aagatatcag catagattgg cacaccaatg agcgtggcaa gcacaactcc gaatggattg
     3961 cggctaccga gcaccgagga aatccacgct tccggaatcc aattatggat cactgtccct
     4021 at
//
LOCUS       DAOSOB010000096         3943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_23068_length_3943_cov_79.966821, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000096 DAOSOB010000000
VERSION     DAOSOB010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23068_length_3943_cov_79.966821"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1160
                     /gene="trpS"
                     /locus_tag="PLS88_08085"
     CDS             114..1160
                     /gene="trpS"
                     /locus_tag="PLS88_08085"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014695921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOU07342.1"
                     /translation="MQKKRILTGDRPTGKLHLGHYVGSLANRVRLQDEYECFFIIADL
                     HTLTTRPEKENIERLPENIRSAVLDYLSVGIDPNKSVIYLQSAVPEVTELSLFFGNLI
                     TVPRLSRVPSLKEMAQNAHIEEMPFGLLGYPVLQAADILLPRAHLVPVGKDNEAHVEV
                     TREIARRFNYLYGETFPVPEVMVSEYGSLVGTDGNTKMSKSLNNAIFLSDDAKTVYKR
                     VMSMYTDPKRIRADIPGTVEGNPVFIYHDAFNQNTAEVEELKARYRAGTIGDVEVKEK
                     LAKVLNDFLDPIRERRAYYESQKGLVEEIIYDGTLRMREEAKITLSMAKKAMGISSVW
                     NRISRKAEERKKHE"
     gene            complement(1168..2601)
                     /locus_tag="PLS88_08090"
     CDS             complement(1168..2601)
                     /locus_tag="PLS88_08090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOU07343.1"
                     /translation="MKLDASILKDFFLDSSLSDFFKEIDIFYIDFLSHTAQSCTTLRS
                     LGYEDADLLKVMENFPVHESDRDRANLQLQRIWSGQIETFIDAFRLASKDGSSYRWLR
                     FTVKVLLRNSEKRPKIVVGHVVDLDDLITSQEEIRERLVEIDTMRELFTAINKSLDFS
                     ETFSRTIDQLRRIIPFDRSCVESLEDGRLIAVAGYGYNESDLSGLVFPAHGVENPAVR
                     AIEERRIVICNDVQQSFPGFINVADEFVTMSWLGIPLIYEKKVIGLLTLDNKELNAYT
                     DQHVRIASELSDYIAIALEHARRHQLVTRQAMTDKLTGLANRYGLETHGIEIFQKAIE
                     EEHSIGVLIIDIDHFKQINDTYGHSYGDVVLQTIALTIRGQIRTRDYAIRYGGEEFVV
                     LLPELGTREALIVAERLRNKIAQIKMEDNKKMPTVSIGIYAAIPGTLDILHEFIRKAD
                     MALYAAKEAGRNRSRVWSISPEFYGEK"
     gene            2643..3929
                     /locus_tag="PLS88_08095"
     CDS             2643..3929
                     /locus_tag="PLS88_08095"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HOU07344.1"
                     /translation="MKKPLNEISNLTFSAQLNSWYEISSSIGLKLCKNIETSIISKNL
                     RSLQRGLTGDRKLIASSYLDQQRILQSYLLYYWPISFYEVSALLYELRTRAVLPKIHS
                     VLDLGSGPGSASFAAQIFGAIRASLVDKSKHALAAASAIAAQGKVNGLNLEIIEHCAG
                     IEDFCAPKGESYDLIIAANSINELWPNSPEGILKRRDIILRFLPNLRPEGIIIIVEPS
                     AHYTSIPLLELRDSLLAADLDGISLACVGPCLHSFQCPMLSKEKKPCFSEWQWNLPPL
                     VRELADCAGLDRDSLKASWIALKKHEKQEKDKEALPDNETSSLDSSSADSLYSAADLG
                     DSGDLFGFRGLRGRIVSEPMLNKAGRVRYIVCTNSALRVTISALRYDPTAENLGFFGL
                     GRGDLIAANGLEIRGESHFGIAPCSQLEVLMRAPRI"
BASE COUNT         1087 a          888 c          906 g         1062 t
ORIGIN      
        1 ttatacaagg ttcgtagcag taatgaagtt atctgctcat tgcccttttt tttctgtttc
       61 gctaaactgt ttcgacactt cataattcag ataaagagaa tcggagaatt accatgcaaa
      121 aaaagcgcat tcttacgggc gataggccta cgggtaaatt gcatctcggt cattatgttg
      181 gctcgctggc gaatcgagtg cgccttcagg acgaatacga gtgcttcttt ataatcgcgg
      241 atctccatac gctcaccact agaccagaaa aagagaatat agaacgactc cccgaaaata
      301 tacgctcagc cgtactggat tatctctcgg tcggcataga tccaaataaa tcggtgatct
      361 atctgcagtc agcggttcca gaagtaaccg aactctcgct ttttttcgga aatctaataa
      421 ctgtgccgag gctctctcgc gtgccaagtc tcaaagaaat ggcacagaat gcgcacatag
      481 aggagatgcc ctttggactt ttgggatatc cagtgctgca ggcagccgat attcttcttc
      541 ctcgcgcaca tctcgttcca gttggtaaag acaacgaagc tcatgtcgaa gtcacgagag
      601 aaatcgcaag acgcttcaac tatctctatg gtgagacctt tcccgttccg gaagttatgg
      661 tctcggaata cggttcgctc gttggtaccg atggaaatac caagatgtcc aagagcttga
      721 acaatgcgat ctttctttcc gatgatgcaa aaaccgtata caaacgtgtc atgtcgatgt
      781 atacggatcc aaagcgtata cgggccgata ttcctggaac cgttgaagga aatcccgtct
      841 tcatctatca cgatgctttc aaccaaaata cggccgaagt cgaggagcta aaagcgcgct
      901 atagagctgg caccataggc gatgtcgaag taaaagagaa actagccaaa gtcctcaatg
      961 atttcttaga tcccatacgc gaacgacgag cttactacga atcgcaaaaa ggtcttgtcg
     1021 aagaaatcat atacgacggc actcttcgta tgcgcgaaga ggcaaaaata acgctttcca
     1081 tggcaaagaa agcaatgggt attagctcgg tgtggaaccg tatttctaga aaggcagaag
     1141 agcgaaaaaa acacgagtaa attttgccta tttttctccg tagaattcag ggcttatcga
     1201 ccagactcga gagcggtttc tacccgcttc ttttgctgca tatagagcca tatcggcttt
     1261 cctaataaac tcatgcagta tgtcgagggt accaggaatg gcggcataga ttcctatact
     1321 cactgtcggc atttttttat tatcttccat tttaatttga gcgattttat tgcgaagcct
     1381 ctcggcaacg atcaaagctt cgcgcgtgcc caattcaggg agcagcacaa cgaattcttc
     1441 tccaccatag cgaattgcat agtcccttgt acggatctga ccacgaatag taagggctat
     1501 tgtctgaaga acaacatcgc cataggagtg tccgtatgtg tcgtttattt gcttaaagtg
     1561 atcgatgtcg atgataagca ccccgataga gtgctcctct tctattgcct tctggaaaat
     1621 ttctatgcca tgagtctcga ggccatagcg gttggcgagc cctgttagct tatcggtcat
     1681 ggcttgcctc gtcacgagct gatgcctgcg tgcgtgctca agagcaatgg cgatataatc
     1741 ggacaactcg cttgcaatcc taacatgctg atcggtatag gcattcaatt ccttgttatc
     1801 cagagtcagc aatccaatca ccttcttttc gtatataaga ggaatgccga gccaagacat
     1861 agtgacgaat tcatctgcaa cattgatgaa gcctgggaac gactgctgaa catcattgca
     1921 aataacgatc ctacgctctt cgattgcgcg cacggctgga ttttcaactc catgagccgg
     1981 aaaaacaagg ccgctgagat cgctttcatt gtagccatag cctgcaacgg ctatcaatct
     2041 tccatcctcg agagattcca cgcaggatct gtcgaagggt atgatacgcc gtagttgatc
     2101 tatggttctc gaaaaagtct cgctgaaatc gaggctcttg tttatagcgg taaaaagctc
     2161 tcgcatggta tcgatctcga caaggcgctc tcgtatttct tcctgggatg tgatgagatc
     2221 gtcgagatct acgacatggc ccactacgat ttttggtctt ttttcggaat tccgcaacag
     2281 aaccttgaca gtaaaacgaa gccatcgata gctcgagcca tctttcgaag ccagccggaa
     2341 agcatcgata aaggtctcta tctgacctga ccagatgcgc tgaagttgta gattcgccct
     2401 atctctatcg gactcatgga caggaaagtt ctccataacc ttcagcaagt cagcatcttc
     2461 atatccaaga gatcgaagag tggtacagga ttgtgcggtg tgtgagagaa agtctatgta
     2521 gaagatatct atttccttaa agaaatcaga aagtgaagag tctaggaaaa aatcctttag
     2581 gatagaagca tcgagtttca ctatactata gaattatata gtatagtaat gatgctgtat
     2641 atatgaaaaa gccattgaat gaaatatcaa acttgacttt ctctgcacag cttaatagtt
     2701 ggtatgaaat aagttcatct ataggtctca agctatgcaa aaatatagaa acatccatta
     2761 tctccaaaaa tcttcgctcg cttcagcgcg gccttactgg agatagaaaa ctcatagcat
     2821 cttcatattt ggatcagcaa agaattttgc agtcctactt gctatactac tggcctatct
     2881 ccttttatga agtatcagca ttactctatg agctgagaac gagagccgtg cttcccaaga
     2941 ttcattctgt gttggatttg ggcagcggac ctgggtctgc ttctttcgcg gctcagattt
     3001 ttggagccat acgagcaagt cttgtggata agagcaaaca tgcactggcg gcggcctctg
     3061 caatagctgc ccaagggaag gttaatgggc ttaacctgga gataatagaa cattgtgctg
     3121 ggatcgaaga cttctgcgct ccgaaaggtg aatcctatga cctgattatc gcagccaata
     3181 gcataaatga gttgtggccc aattcgccag agggaatttt gaaacgacgt gatatcatac
     3241 taagattttt gcccaatttg cggccagaag gaatcataat aatagtggag ccttccgctc
     3301 actatacgag cattccgctc cttgagttgc gcgatagttt gcttgcggca gatttggatg
     3361 gtatatctct agcatgtgtt ggtccttgtc ttcattcctt ccaatgcccg atgctgagca
     3421 aagagaaaaa accttgtttc tcagaatggc aatggaatct tcctccttta gtgcgtgagc
     3481 tcgcagattg tgcagggctc gaccgagatt ctctcaaagc ttcgtggata gccttgaaaa
     3541 agcacgaaaa acaagagaaa gacaaagaag ctctgcctga caacgagaca agttctcttg
     3601 actccagcag tgcggacagc ctttatagcg cggctgatct tggtgattct ggtgatttat
     3661 ttggttttcg cggacttcgc ggtcgcattg tatctgagcc catgctcaac aaggctggaa
     3721 gagttcgcta tatcgtgtgc acaaatagcg ctttgagagt gacgatttcg gcgcttcgat
     3781 acgacccgac ggctgaaaac ctaggttttt tcgggcttgg acgtggcgac cttattgctg
     3841 caaatggtct agaaattcgg ggtgaatcgc actttggtat tgcgccttgc tcacagctcg
     3901 aagtattgat gagagcccca aggatttaga aagcaaacaa aat
//
LOCUS       DAOSOB010000097         3915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_23274_length_3915_cov_79.176166, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000097 DAOSOB010000000
VERSION     DAOSOB010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23274_length_3915_cov_79.176166"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..3310
                     /gene="ileS"
                     /locus_tag="PLS88_08100"
     CDS             131..3310
                     /gene="ileS"
                     /locus_tag="PLS88_08100"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOU07345.1"
                     /translation="MYKPVDPKVNFPKQEESIVQFWEEQNIFQKSVSQREGAPEFSFY
                     DGPPFATGLPHFGHFVPGTIKDIFPRYKTMRGYHVERRFGWDCHGLPVENLIEKELGL
                     NSKTDIERYGIAEFNEACRGSVLRYVKEWRQIVTRLGRWVDFDHDYKTMEPDYMESIW
                     WVVKSLWDKGLIYEGHYILPYCPRCSTVLSNHELSLGGYEDVSDPAITVRFRITGLPI
                     SLDDPSLADLADGSTYFVAWTTTPWTLVSNLALCLGPDIDYACVIDGKERYILAESRL
                     SDYYKNPESLKFSWKKKGSELTGIRYEPLFPYFAFLAEKGAFVTVLGQHVTTEEGTGI
                     VHTAPGFGEEDYEVLRGSSIPTICPVDEECRFTEEVPDYKGLFVKDADKAIMNRLKNE
                     GKLVKRDQIVHAYPHCWRCGSPLIYRAISSWFVEIEPIKKSMLRANKSIYWVPAHIRD
                     GRFGKWLENARDWAISRNRYWGNPLPIWKCDECGKTICVGSRKELAELGGKVPEDLHK
                     HYLDRVTLPCSCGGTMHRIPEVLDCWFESGAMPYGQNHYPFENKQKFESNFPADFICE
                     GLDQTRGWFYTLTILAAALFDKPAFKACVVNGLVLAEDGKKMSKSLRNYTDPSEVMDK
                     FGADALRLFLMNSAVIKAEDLCYSDEGVKDVLKSVILPLWNAYSFFVTYANIDGFVAE
                     DLDCTQVENPLDKWILSVCEKMIADVTTGFETFDLQAAIAPTVEFVDSLNNWYIRRSR
                     RRFWKSENDTDKKNAYKTLYRVLRRLTLVMAPVIPFVTEAIYQNIKLQMEPESIHLSD
                     WPKTEEKYRNLELERDMRIVRQAVTMGRSLRVANDIKIRQPLASIQLISRVPEEREVL
                     ARMEDILREELNVKAVVIHEKEEDLVEYHAKANFRVLGKELGSYMKEAAEKIANLSSK
                     EIARLLDEEHILLTLSSGKELILDSSRVDIIREEKQGLKVLNEGSLTVALDTNIDSEL
                     LYEGYVRDLIRGIQNARKEKGLDVTDRIILEVSGDDELRAALDRFGQMVADETLALRI
                     DWKDIDKAMINGFTVEAGDKAWIFNLEKA"
     gene            complement(3307..>3915)
                     /locus_tag="PLS88_08105"
     CDS             complement(3307..>3915)
                     /locus_tag="PLS88_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07346.1"
                     /translation="SLVKYDLPEDSGNSDSESMKNVSKEDRSSRVQYLLPPIEYYEIQ
                     YPIYISENIICVATQYYLFNGGAHGSASTDLDIIDRKTGKRLEVGDVLVGDWKIALGP
                     KLKAELLRQSRYWKASGSAAKVQNDKNISIEDLRELGLFESDIYPSDKIFLCKTGIGF
                     EYDQYELAPRYFGEFIILLPWDELKPFLNPAFFTDGQILGSI"
BASE COUNT         1146 a          862 c          868 g         1039 t
ORIGIN      
        1 ccttcttatt ttgcaatatt tttcgcgatt cttgcgcatt tttgtttcca tcatcttcgc
       61 atttaacttc ctcctcttga aaggatttgc aacatacagc attattcgtg acattatcga
      121 ggaggatatc gtgtataagc ctgttgaccc caaggtcaac ttccctaagc aggaagaatc
      181 gatcgttcaa ttttgggaag aacagaatat attccagaag tcagtttcgc agcgtgaagg
      241 tgcaccagaa ttcagtttct atgatggccc tccctttgca actggactcc ctcactttgg
      301 ccactttgta ccaggtacga tcaaagatat tttccctcga tacaaaacaa tgcgaggcta
      361 tcatgtagaa aggcgctttg gatgggattg ccatggctta ccagtagaga atcttatcga
      421 aaaagaactt ggcctaaatt caaagaccga cattgagcgc tatggcatcg ccgaattcaa
      481 tgaggcttgc cgaggttctg tccttcgata tgtaaaagaa tggcgccaga tcgttactcg
      541 cctcggccgc tgggtcgatt tcgatcatga ctataaaact atggaacctg attatatgga
      601 atcgatctgg tgggtagtca aatccctatg ggacaaggga ctcatatatg aaggccacta
      661 tattctccca tattgtccgc gatgttctac agtattatct aatcatgagc tttcactggg
      721 gggatatgag gatgtaagcg accctgcgat tactgtgagg tttagaataa caggcctgcc
      781 aatatcgcta gatgatcctt ctcttgcaga tctagctgac ggatctacat atttcgtagc
      841 atggacaact accccatgga cactggtttc aaacctcgct ctttgtcttg gcccagatat
      901 cgattatgcc tgtgtaatcg atggaaaaga acgatatatt cttgctgaat caagattaag
      961 cgactattat aagaatcctg aatcattgaa attttcatgg aagaaaaaag gcagcgaact
     1021 taccggaatt cgatacgaac cactattccc ctacttcgcc ttcttggctg aaaaaggtgc
     1081 atttgtgacc gtacttggcc agcacgttac tactgaagag ggaactggaa tagttcatac
     1141 agcaccaggt tttggtgaag aagattacga agtattgcgt ggctccagca ttcctactat
     1201 atgcccagtc gacgaagaat gcagatttac tgaagaagtt ccagattata agggattatt
     1261 cgtaaaagat gcagacaaag cgataatgaa tcggcttaaa aatgaaggca aactcgtaaa
     1321 gcgcgaccaa atagttcacg catacccgca ttgctggcgc tgtggaagcc cacttatcta
     1381 ccgtgcaatc tctagctggt tcgttgagat cgagccaatc aaaaaatcca tgcttagagc
     1441 caacaaaagc atctactggg tacctgccca tattcgcgat ggtcgattcg gaaaatggct
     1501 agaaaatgcg cgagactggg cgataagccg aaatagatat tggggcaacc cgcttcccat
     1561 atggaaatgc gatgaatgcg gaaagaccat ctgtgtaggc tctcgtaaag aacttgcaga
     1621 gctcggcggc aaagtgccgg aagacctgca taagcattat ctcgatagag ttacgcttcc
     1681 atgcagctgc ggtggaacca tgcatcgaat tcctgaagtt ctcgattgct ggttcgaatc
     1741 tggagcaatg ccatacgggc agaatcatta tcccttcgaa aacaagcaaa aattcgaatc
     1801 caacttccca gcagatttta tctgtgaggg tctcgaccaa acgcgaggct ggttttatac
     1861 attgacaatc cttgccgcag ctctttttga caagcctgcc tttaaggctt gtgtcgtcaa
     1921 tggccttgta ttggccgaag atggcaaaaa gatgtctaaa agcctaagaa actacaccga
     1981 tccttccgaa gtaatggaca aattcggtgc cgatgcccta agactctttc tcatgaattc
     2041 agctgtcata aaggctgaag acctttgcta ttcagacgaa ggtgtcaagg atgttctaaa
     2101 gagcgtaatc ctcccacttt ggaatgccta tagttttttt gtcacatacg caaatattga
     2161 tggctttgtg gctgaagatt tagactgcac gcaagtcgaa aatcctctgg acaaatggat
     2221 actatctgta tgcgaaaaaa tgattgccga tgtgacgacc ggtttcgaaa ccttcgatct
     2281 acaagcagcc atagccccga ctgtcgaatt cgtggacagt ttaaacaatt ggtatatccg
     2341 tcgttcgcga cgcagattct ggaaatcaga aaacgataca gataagaaga atgcctataa
     2401 gaccctctac cgtgttcttc gccgacttac cttagtcatg gcgcccgtaa ttccatttgt
     2461 cacagaggca atctatcaga acataaagct tcaaatggaa cccgaatcga tccatctgtc
     2521 agactggcct aaaacagaag aaaagtatcg caacctcgaa ttagagcgcg atatgcgcat
     2581 tgtgcgacag gccgttacta tggggcgatc tctcagagtg gccaatgata ttaaaatacg
     2641 ccagcctctt gcatcgattc aacttatcag ccgagttccg gaagaacgcg aagttttggc
     2701 gcgaatggaa gacattctcc gagaagaact caatgtcaaa gctgtggtca tccatgaaaa
     2761 agaagaggat ctcgtcgaat atcacgctaa agccaatttc cgtgtactgg gcaaagagct
     2821 tggttcttat atgaaggaag ctgcagaaaa aatcgccaac ctgagcagca aagaaattgc
     2881 tcgtctccta gatgaggagc atatcttgct caccctgagc tcaggcaaag aattaatcct
     2941 agatagttcc cgagtagata taatccgcga ggaaaaacaa ggcctcaagg tcctaaatga
     3001 aggtagcctt actgtagcgc tcgataccaa tattgactcc gaattgctct atgaaggata
     3061 tgtgcgcgac ttaatccgag gaatacagaa tgcccggaag gaaaaaggtc tcgatgtgac
     3121 cgataggatt atcctagagg tttccggcga tgatgaactt agggctgcac tggaccgctt
     3181 cggtcaaatg gtagctgatg agaccttagc cctaagaatc gattggaagg atatagacaa
     3241 agccatgata aatggcttta cagtagaagc aggcgataag gcttggatat tcaacctgga
     3301 aaaagcttag atcgagccta atatctgtcc atcagtaaaa aacgctggat taaggaaagg
     3361 ctttagttca tcccacggaa gcaatatgat gaactctcca aaatatctag gagcgagttc
     3421 gtattgatcg tattcaaagc ctattcctgt tttgcacaag aatattttgt cggatgggta
     3481 tatatcagat tcgaataatc caagctcgcg aagatcttcg atggagatat ttttatcgtt
     3541 ctgaactttg gcagcacttc ccgaagcctt ccaatatctg ctctgcctaa gcaattctgc
     3601 cttgagcttt ggcccgagag ctattttcca atctcctact aaaacatcac cgacttcaag
     3661 ccgtttgcca gtctttcgat ctatgatatc aagatctgtc gaagccgaac cgtgcgctcc
     3721 tccattgaat aaataatact gggtcgcaac gcaaattata ttttcgctga tatatatggg
     3781 atattggatc tcatagtatt cgataggagg caacaaatat tgaacccggc ttgatctgtc
     3841 ttctttagag acgtttttca ttgactcaga atccgagttt ccagaatctt ctggtaaatc
     3901 atatttaaca aggga
//
LOCUS       DAOSOB010000098         3896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_23431_length_3896_cov_84.185108, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000098 DAOSOB010000000
VERSION     DAOSOB010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23431_length_3896_cov_84.185108"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PLS88_08110"
     CDS             complement(<1..986)
                     /locus_tag="PLS88_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07347.1"
                     /translation="MDHEIEMTFQRISNDVLKIFFRAKEPIECQEPLLELGELTVESL
                     CDALGDECIEAEEAAGLGLGSAVEKLSKARGRLVLVNGWQSWSFAGELKGWERPRRAL
                     LRRDLNLFVDHPAERALRLRARRFFHRHDIISHFLLGLRTGELRLMLVSDNVERWSGP
                     RSSDVDRISRADFSGDSSDKSSCPHYLPPVSFLLRGDKIRVFAYASGGYFAAGEIIAR
                     LCVFIGPDYFSLKDRLGSYWAADRRFEELSWVSKPKSISHGMIGGYASWYNHYTAIDE
                     QIIGDDLEAIKANDNFVNSYFLQRGLPAVFQIDDGWEIQVGDWQADSHKFPNG"
     gene            1124..2017
                     /gene="thyX"
                     /locus_tag="PLS88_08115"
     CDS             1124..2017
                     /gene="thyX"
                     /locus_tag="PLS88_08115"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOU07348.1"
                     /translation="MAHCIVPEAEAILDKEFPVLDKGFVRLVDYLGGDQRIVQAARVS
                     YGEGTKSYREDAALIDYLWRHEHTSPFEQVVLTFHVKLPIFVARQWIRHRTARVNEIS
                     GRYSIMRDEFFLPAPDALAPQSEDNKQGRSDMPFDEQIASKIQDILKEGANLSYERYR
                     ELIDLGLAREVARIALPLSLYTEWYWQMDLHNLFRFLMLRLDPHAQREIREYAKVILE
                     ITRKVAPAATASFERHTLEAVRFSGPEMMELRRRLKQGEALGDAAECVEPRPCQEDQP
                     TKLSKKELERFEEKLKTGRQL"
     gene            complement(2201..>3896)
                     /locus_tag="PLS88_08120"
     CDS             complement(2201..>3896)
                     /locus_tag="PLS88_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOU07349.1"
                     /translation="KIEADEERRNGFILAALTTMRTFLMQRENIQKTSELSILNREAL
                     EIGKKTSLQEIADTALDILDKEKNWRPSVIRFKSRSGEVLETIAYRSQLPIGAEEVQQ
                     RMNLFNEMIYRPGLGMTGYVIESGKPIRCLDLPSDPHYVQTDLNIKYGIYAPISIEGS
                     VEGAIGIESKDYVFKDSDLRLLQSISEIVGIAARSVRLIEVLRERLHWLEILHKINEQ
                     IGIETEPDKLYKTLVDSAIEATQAESAALLIYNPETDLLEKAAAKSWLDKVFEIPLKP
                     DESISGGIFQSGKTRLSPEIREDPLIIPRNLPLIPAGCANIGVPVVYEDKVLGVFHLS
                     IKAPLSFTKELVEFAEMFGSYSGIVIGRMQQIQALQEAEKQIKIAYDETLEGWARAIG
                     YRDNETFQHTLRVTNIANIIGEALSLDDKTLENLRRGAILHDVGKIGIPDNILRKPRA
                     FTKEEKEIMRTHPSFAYELLRPIAFLSEAIIVPYCHHERWDGKGYPQGLKGDEIPLLA
                     RIFSVADVYDAMTSDRPYRKARTHEEAIKYIRSQSGRHFDPHIVDVFLEIVDKIKD"
BASE COUNT          952 a         1013 c          899 g         1032 t
ORIGIN      
        1 ccattcggaa atttgtgaga atcggcttgc caatctccta cctgaatctc ccaaccatcg
       61 tcgatctgaa atacagcagg taagcctcgc tgcaagaaat agctgttgac aaaattatca
      121 ttggccttga tggcctcgag gtcgtctccg ataatctgtt cgtcgattgc cgtatagtga
      181 ttgtaccagg aggcatagcc gcctatcatg ccatgggaaa tggactttgg tttgctgacc
      241 caactcaatt cctcgaaacg ccgatcggca gcccaatagg agccaagcct gtccttaagc
      301 gaaaagtagt ccgggcctat gaacacacaa agacgggcaa ttatctcgcc agcagcaaaa
      361 tagccgcccg atgcataggc aaaaacacgg attttatcgc ctcgaagcaa gaaactgaca
      421 ggaggaagat agtgtgggca agagcttttg tcgctcgaat cgcccgaaaa atcagctcta
      481 gaaatgcggt ccacgtcgct ggagcgaggg ccactccagc gctccacatt gtctgacacg
      541 agcattagcc tcagttcgcc tgttctaagc ccgagcagga agtgcgagat aatgtcgtgc
      601 ctgtggaaga agcgccgcgc ccgcaggcgc agcgcccgct ctgcagggtg gtcgacgaac
      661 aagttcagat cccggcgcaa cagggcgcgg cgcggcctct cccagccctt cagttcccct
      721 gcaaacgacc aagattgcca gccatttacc agcaccagcc gccctcgcgc tttagaaagc
      781 ttttccacag cgcttcctaa tccaagccca gcggcctctt cagcctcaat gcactcatcg
      841 cctagcgcat cgcataagct ctccaccgtt agctcgccga gctccaagag tggctcttgg
      901 cattctatcg gctcttttgc tcgaaaaaag atcttcagca catcgttgga gattctctgg
      961 aatgtcattt cgatttcatg atccataagg ctatggtatc acagcaagcg atttggaagg
     1021 agagtcataa aataaattct gcatctatca atttagcgca ttatgttctt ttatttgaac
     1081 cccaatacct agctactatc tcaatttctg ttacaatatg cctatggcac actgtatagt
     1141 tcccgaagca gaagcaattc tcgataaaga attccccgtt ctcgacaagg gctttgttcg
     1201 gcttgtcgac tatctaggag gcgatcagcg tattgtgcag gccgcccgag tttcctatgg
     1261 tgaaggcacc aagagctatc gcgaagatgc cgctcttatt gattatctct ggcgccatga
     1321 gcacacaagc cccttcgagc aggttgtgct caccttccat gtaaagctgc cgatcttcgt
     1381 ggctcggcag tggattcgcc accgcaccgc acgcgtcaac gaaatatccg gtcgttattc
     1441 cattatgcga gatgaattct ttcttcctgc tcccgatgcc ttggcgccac agagcgaaga
     1501 taacaagcag ggtcgctccg acatgccttt cgacgaacag atagcatcga agatccagga
     1561 tattctcaag gaaggagcca atctttccta tgaacgctat agagaactca tcgatctagg
     1621 ccttgcccgc gaagtagccc gcatagctct gccgctttct ctgtataccg agtggtattg
     1681 gcagatggat ctgcacaatc tttttcgttt tcttatgcta aggctagacc ctcatgcgca
     1741 gcgtgaaatc cgcgaatacg caaaggtaat cctcgaaata accagaaagg tcgcgcctgc
     1801 ggcaaccgct agtttcgagc gacatacact ggaggctgtg cggttctccg gccctgagat
     1861 gatggagctt cgacgaagac taaaacaagg cgaagccttg ggagatgcgg cagaatgcgt
     1921 cgaacccaga ccctgccagg aagaccagcc aaccaaacta tctaagaaag agctcgagcg
     1981 cttcgaagag aaacttaaga ctggccgcca gctttaagta acataatcaa cccgcaaaca
     2041 tgttcgccag ctttaagagc tttaagcggc tctgctttgc gacctagcac acttttaagg
     2101 cacccaatct cggttaatat ctctgcttca atatattgcg atcttatggc cactgttttt
     2161 atatatccag ctttggagcc tagctcagat cttaccttgc tcaatcctta atcttgtcta
     2221 cgatctccaa aaatacatcg acgatatggg gatcgaaatg ccttccagat tgtgatcgaa
     2281 tatactttat ggcctcctcg tgtgtccttg ctttgcgata aggcctgtcg ctggtcatcg
     2341 catcgtagac atcagccacc gagaatatcc ttgcaagaag agggatttca tcgcctttta
     2401 gaccctgcgg ataacccttg ccatcccagc gttcatgatg gcaatagggc acaataatcg
     2461 cctcactcaa aaatgcaatg ggacgcaata gctcgtatgc aaaagatgga tgtgtgcgca
     2521 ttatttcctt ctcttcctta gtgaaagccc taggttttcg tagaatattg tccggtatac
     2581 cgatttttcc tacatcgtga aggattgcgc cacgccttag attctcaagt gttttatcat
     2641 cgagggaaag ggcttcccct attatattag ctatatttgt cacgcgcagc gtatgctgga
     2701 aggtctcgtt atccctatat ccaatggcgc gcgcccagcc ttcaagagtc tcatcgtaag
     2761 ctatcttgat ctgcttttct gcttcctgga gagcctggat ttgctgcata cgcccaatga
     2821 cgattcctga atatgatcca aacatctcgg caaattcgac gagctcctta gtaaaggata
     2881 aaggcgcctt tattgaaaga tggaagactc ccagtacttt atcttcatag actacaggta
     2941 cgccaatgtt agcgcatcca gcaggtatga gcggaaggtt cctaggtatg ataagaggat
     3001 cttctctaat ctcgggcgat aggcgtgtct tgccgctctg gaatattcct ccgcttatgc
     3061 tttcgtctgg cttgagtggt atttcgaaga ccttatcgag ccagcttttg gctgctgctt
     3121 tctctagaag gtctgtctct ggattgtaga tgagaagagc ggcggattct gcctgtgtag
     3181 cttcgatggc gctgtcgaca agtgtcttat agagcttgtc tggttctgtc tctatgccga
     3241 tctgttcatt tattttatgg agtatttcaa gccaatgtag acgttcgcgg agcacttcta
     3301 taagccttac agacctagca gctataccga caatctcgct tatggactgt aagaggcgta
     3361 ggtcagagtc cttgaaaaca taatccttgc tctctatgcc tattgctccc tcaacgctgc
     3421 cttcgatcga tatgggagca tagatgccat acttgatatt taggtcagtc tgaacatagt
     3481 gcgggtcaga tggcaagtcc agacagcgta taggtttccc gctctcgatg acataacctg
     3541 tcatacccag gccagggcgg tatatcatct cgttgaagag attcattctc tgctggactt
     3601 cttctgcacc aataggaagt tggctcctat aggcgatcgt ctctagtact tcgcccgatc
     3661 tagatttgaa tcttataacc gaagggcgcc aattcttttc tttatcgaga atgtccagcg
     3721 cagtatccgc aatctcttga aggcttgtct tcttgcctat ttcgagggcc tccctgttca
     3781 ggatggagag ttccgatgtc ttctgtatat tctcacgctg catgagaaat gtcctcatag
     3841 ttgtcaaggc agcaagaatg aagccatttc ggcgttcctc atcggcttct atttta
//
LOCUS       DAOSOB010000099         3685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_25134_length_3685_cov_100.132231, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000099 DAOSOB010000000
VERSION     DAOSOB010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25134_length_3685_cov_100.132231"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLS88_08125"
     CDS             complement(<1..197)
                     /locus_tag="PLS88_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07350.1"
                     /translation="MNQKSNKPNTMPFRPAWKIERELRLSVIPLDRRTHESNWYTSDT
                     VFPTGDDLIALFWHSSVPGSGA"
     gene            complement(194..1096)
                     /locus_tag="PLS88_08130"
     CDS             complement(194..1096)
                     /locus_tag="PLS88_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU07351.1"
                     /translation="MNIVIGLIVGMISGATPILLAALGGTLTFYAGIFNIAMEGMMLM
                     AAFFAVLGSFLFHSWVIGLVCAIAGAMILALIFIFFSVKLNTDEFVSGIALNLFALGI
                     TTYLLRQIFKVKGVFTSKLITSVPNLRIPFIEKIPVLGPVVSGRNLIVYLAVIATFIC
                     YYLVFKTRFGLRLRAAGYNSNSLDSSGVRTSSMRIQALLLCAVFCGLAGAYLSLGYVT
                     LFAENMTAGRGWISLAAIILVDGHPYGIALISLLFGFFDGLGLFLQSYGVSPQFTSMV
                     PYIATLVALYVYSRRARERERKAL"
     gene            complement(1093..2130)
                     /locus_tag="PLS88_08135"
     CDS             complement(1093..2130)
                     /locus_tag="PLS88_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU07352.1"
                     /translation="MIRKIISGVLGVVIALAVGALIMLLQGYEPLATYGALAQFSIGG
                     LGPFTTTLKNAVPLVLTGLSAAIAFASGPVNLGQPGQFAFGALAATLAGLYTNLPPFL
                     EIPLLMLCAIAGGALWSGLAALLRQAFDMSEFIVTLMLNMIADFFTAWAIAYPFMDKK
                     AFSPMTPPIAKSGWMPEIGSLSSSVIVMLMSVAIMWFVFQRWRAGYEWRITGQNSLFA
                     RIGGCDVRGNFVAVMLVTGALAGLAGGLVVMAGPHRFIKGLGANYAWDGIMVAIMANN
                     GLVETLIYGIFVSAIQTGALGMELITNVPSEIAQVLQAVLVLVIVATREYAVLAFDKL
                     AARRQAREHAA"
     gene            complement(2127..3656)
                     /locus_tag="PLS88_08140"
     CDS             complement(2127..3656)
                     /locus_tag="PLS88_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU07353.1"
                     /translation="MDDFISLEHIVKVYPPNVLAVDNVSVGFRKGEIHSIIGENGAGK
                     STLMKILYGLVPFDSGTVRLNGNAVHFKKPADAIAMGIGMVHQEIELISQYKVWQNVV
                     LGAEPIRGSAGKLDVKQAIELVRAKVDEFQFNIDPEAIVDRISVAARQKVEILKLLYR
                     NVSVLILDEPTSVLAPQEIPQLFSELKRLRDKGTTILFISHRLDEVLELSDRITVMRK
                     GKKIATVAAAETSKAELAQMMVGRQVIFTSKRAPHESGSKMFETKNLNLVRRDGLKLL
                     DDISFSVHAGEIVGIAGVEGNGQFELVNCIMGLTEPSGGHIEVGNKEITRLTILERRK
                     LISFVPQDRAKMGASIPASILDNAIMTHHRLNPDFSKWRGLVLNYRTARTFVESLAER
                     FSVQMASYQNPFRSLSGGNQQKAILGRELLLPTPFFLMDQPTRGLDVGSIEYVHDQIL
                     SMRQENRAILVISADLEELFLIADRILVMHRGRIVADLVTDQTSIEEVGRYMLEGKLT
                     A"
BASE COUNT          998 a          966 c          884 g          837 t
ORIGIN      
        1 gctcccgaac cagggacgga ggaatgccaa aacagagcta tcagatcatc gcctgtaggg
       61 aacaccgtat cgctcgtata ccaattgctt tcgtgcgttc tgcgatccag aggaatgacc
      121 gaaagcctga gctctcgttc gatcttccaa gcaggacgaa agggcatcgt attcggcttg
      181 tttgatttct gattcatagt gctttccttt cgcgctcgcg ggcacggcgc gaatatacgt
      241 agagggccac gagagtcgct atatatggca ccatcgacgt aaattgtggg ctgactccat
      301 agctctgcaa gaaaagccca agcccgtcga aaaagccaaa gagcaacgaa atcagcgcta
      361 tcccataagg atgaccatcc accagaataa tcgctgccaa ggaaatccat ccgcggccag
      421 ccgtcatgtt ctcggcaaag agagttacat agcccagcga aagataggct cccgccagcc
      481 cgcagaatac agcgcacaat agaagagctt gaatcctcat gctagaagtg cgcaccccgc
      541 tcgaatctag cgagttcgaa ttatagcccg ctgctctcag cctcaagcca aaacgtgtct
      601 tgaatacgag ataatagcag ataaatgttg caatcacagc aagatacacg attaggtttc
      661 taccggatac aacgggcccc aacacgggga tcttctcgat aaacgggatt ctcaaattcg
      721 gaaccgatgt gatgagctta gacgtgaaaa caccttttac cttgaatatc tgtctcagta
      781 gataagtcgt aatccccaac gcaaagaggt taagggcaat tccggataca aattcatcgg
      841 tattcagttt tacggaaaag aaaataaaga tcaaagctag aatcatagct cccgctatgg
      901 cgcatacaag cccaatcacc caggaatgaa acaagaaaga gcccagaacc gcgaagaatg
      961 cagccatgag catcatgcct tccatggcaa tgttgaagat gcctgcgtaa aatgtaagag
     1021 ttccgccgag tgcagcaagc agaatgggag ttgctccgga aatcatgcct actatcaaac
     1081 caattacgat attcatgctg catgctccct tgcttgacgt cgggctgcga gtttatcgaa
     1141 ggccaacact gcgtattcgc gcgtagccac tatgacgagc acgagcaccg cttgaagcac
     1201 ctgcgctatc tcgcttggaa cattagttat taactccatg cccagcgctc ccgtctgaat
     1261 cgccgataca aagatgccat agatgagcgt ctccacaagc ccattgttcg ccataatagc
     1321 gaccattatt ccatcccatg catagttggc gccaagccct ttgatgaatc tatgcggacc
     1381 ggccattacc acaagcccgc ccgcaagccc ggcaagtgca ccggtgacca gcattaccgc
     1441 gacgaaattg cctcgcacat cgcagccgcc aatacgcgca aagagcgaat tctgacctgt
     1501 gatgcgccac tcatagcccg cgcgccagcg ctgaaaaacg aaccacatga tagccacaga
     1561 cataagcatc acaataacag atgagctcaa agatcctatt tcaggcatcc agccactctt
     1621 ggcaataggc ggggtcatgg gcgagaatgc cttcttgtcc ataaaaggat atgcaattgc
     1681 ccatgcagta aagaaatccg caatcatgtt gagcattaga gtgacaatga attcgctcat
     1741 gtcgaaggct tggcgtaaaa gggccgctag acccgaccac agcgcgccac ctgcgatagc
     1801 gcatagcatg agaagtggaa tttcgagaaa tggcggaagg tttgtataga gccctgccag
     1861 cgtcgccgca agagctccga atgcgaactg cccgggctgt cctaggttga caggcccgga
     1921 ggcaaaagct attgctgcgg acagtccagt tagcacaagc ggcacggcgt tctttagggt
     1981 agtagtaaat ggcccaagcc cgccaatcga aaattgcgcc agagctccgt atgttgctaa
     2041 aggctcataa ccttgcagca acatgataag agcacctacc gccagcgcga ttacgacacc
     2101 aagcacacca ctgattattt tgcgtatcat gctgtaagct tcccttctag catatagcgc
     2161 ccaacttctt cgatcgaggt ctggtcggta acaagatccg ccactattct tccgcgatgc
     2221 atcacgagta ttctatctgc gattaaaaaa agttcttcga gatccgcaga gatgacaaga
     2281 atggctcgat tttcctggcg catagagaga atctgatcat gcacatactc gatcgatcca
     2341 acatctagcc ctcgagttgg ctgatccatc aagaagaaag gagtgggcaa caatagctcg
     2401 cggccaagga ttgccttttg ctggttgcct ccagatagcg atctaaatgg gttctgatat
     2461 gatgccatct gcacagaaaa gcgctcggca agagattcga caaaagtccg cgcagtgcgg
     2521 tagtttagta caaggcctct ccatttcgaa aaatccggat taagtcgatg gtgtgtcatt
     2581 atggcattgt ccaatatcga agcgggaata ctagctccca tcttggccct atcttgcggc
     2641 acaaaggaaa tcaatttgcg ccgttcgaga atggtaagtc gagtgatttc cttgttgccg
     2701 acttctatat gcccgccgct tggctcggtc aatcccatga tacagttgac cagttcgaac
     2761 tgaccattgc cttccacacc tgctatgccg acaatttcgc ctgcgtgaac cgaaaacgaa
     2821 atatcatcaa gaagtttaag accatcgcgt cgcacaaggt tcagattctt cgtttcgaac
     2881 atcttcgatc cgctctcatg aggagcgcgc ttagaggtaa agatgacctg cctgccgacc
     2941 atcatctgag cgagctctgc cttgcttgtt tccgcagcgg ccacagttgc gatttttttg
     3001 cccttacgca tgacggttat tcgatcgctg agctcgagca cttcatcgag ccgatgggaa
     3061 atgaagagaa tcgtagtgcc tttgtcgcgc agacgcttta gttcggaaaa aagctgagga
     3121 atttcttgag gagccaacac cgatgtcggt tcgtctagta tgagaaccga gacatttcgg
     3181 tagagcagtt tgagaatttc gaccttctgg cgagcggcga cggagatgcg gtcaacgatg
     3241 gcctcgggat cgatgttgaa ctgaaattcg tcgacttttg ctcgcacgag ctcgattgct
     3301 tgcttgacgt ctagcttgcc tgcagagccc cgaatgggct ccgcgccgag cacgacattt
     3361 tgccagacct tgtactgaga gatgagctcg atttcctgat gaaccattcc aatgcccatc
     3421 gctatcgcat cggcgggctt cttgaagtga acagcattgc cgtttagacg gacggtaccg
     3481 gaatcgaatg gcactagacc ataaaggatc ttcatcaacg tgcttttgcc agcgccgttc
     3541 tctcctatta tcgaatgtat ttcgcctttt cgaaatccga cggagacatt gtccaccgcg
     3601 aggacattgg gcggatatac tttgacaata tgttcgagcg aaataaaatc atccatgagt
     3661 tgccactttc aattaaaaat caatg
//
LOCUS       DAOSOB010000100         3528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_26641_length_3528_cov_61.218831, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000100 DAOSOB010000000
VERSION     DAOSOB010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26641_length_3528_cov_61.218831"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..1212
                     /locus_tag="PLS88_08145"
     CDS             127..1212
                     /locus_tag="PLS88_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07354.1"
                     /translation="MKKTWFFSKYLMGAVIIGLLIISCATPPPAEKPSSESQRAKEIV
                     QGTSTPEMPSTTAPGDVQSAAKAVVESQPIPGETQAPSTPSSSPQASASYYTQPMTAE
                     ESRFFQNYINRLSYLVYYNENSGLDPQLAKAAVSQANRYLIEKEGLSVIDFDQIQKNK
                     KDQISAYQAETGGSIDIITYIAQKLNADIYLEIDAKTSFGGVPGAWTGSAQGSIKIFD
                     ASTAALLGSISFLSPQTFSPVSPDAAMMNAISGIIWQSMPKVTEQAKHLMSTLTAARG
                     VRYEIILQNTPDAMAVSQFERNLAKRVREVERLSYSPGETRFALYAFMPASKIQDTIY
                     DVASMSGFPNCYLLYMRGRSYTFNTGI"
     gene            1248..1901
                     /locus_tag="PLS88_08150"
     CDS             1248..1901
                     /locus_tag="PLS88_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07355.1"
                     /translation="MKARKVVLIVLVAAVVISCGTKPPLRKGEVLIEDKGTAYGVKTP
                     KWVELAIVGGYRDIEKLPDYKDKVVFIAQFEAQNLQSAQLLAERMQADTEIARYLSTR
                     VKDAFKGANVADADSKNFGAYGERFVASVGEAKFSGFRMEADWWVKVQTYTPENKPDK
                     QIYRVIQLWAIDKNMLQKQFDILFSQMAGGEPATPEQKRAMDLVQNTVAKDFFGESH"
     gene            1998..3455
                     /locus_tag="PLS88_08155"
     CDS             1998..3455
                     /locus_tag="PLS88_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07356.1"
                     /translation="MKKSIIIWMVIFFLLVASCASAPSIPEWVKKTPSPDSKYTYFVG
                     SSSAKDSATALNDATAALIAGIMQYMGVSISVESSAEAKASLSEYEAQITQTVKTESK
                     GRLSGFEVVEKYIQSDKKTGLYTVHVLARYETKELNKEKARIESLLKEKADAVAIPEQ
                     KGDSAANEGHLIDAIRAYAEAMSAAGSSDIENAQIKLERNAKKASALAATLKLVVLSP
                     SNQEIPLGSTNAPSFVVKLITNRRGIEEGVPGAPLVVTYPRRLTSGRMGTTTQQVFTN
                     QDGIVSFDVPVVELAGDYQVTVQLDFGSIADILGGLPGWALPYMEALESDLSGIRVYM
                     SYKVVSAAKSVPTAIGVYLKDQTQKGSLDPETFVKSLEEALVKEGFSIFTTKISAEYG
                     KPDIAQLKAIAPTQAKRFILATVELVSINKEDSFFIASVTGSLSAIDLIEGNTLYSGS
                     KGAQGMGFSEAEAISAALRTLGGQVFAKELLAALR"
BASE COUNT         1069 a          702 c          829 g          928 t
ORIGIN      
        1 gtcggcctcc ggagccgaag gtcgcgcgtt caaatcgcgc atcgctcaga gaaaagtctt
       61 cgattattat atcttatttc tcatttctat cttgcaaaaa cattaaagct gtccaataat
      121 aatagcatga aaaagacatg gttttttagc aaatatctta tgggtgctgt gattattggt
      181 ctccttatta tcagttgtgc aacgcctcct ccggcagaaa aacccagttc ggaaagtcag
      241 agagcaaaag aaattgttca gggtacgtcg acaccagaaa tgccgagcac gacagctcct
      301 ggagacgttc agtctgctgc aaaagcggtt gtagaaagcc agcctatacc tggcgagact
      361 caagcgccat caacgccttc ttctagtccg caggcaagcg caagttatta cacgcaacca
      421 atgaccgcag aagaaagcag gttctttcaa aattatatta atagactcag ctatctagta
      481 tattacaatg agaatagtgg cttggatcca cagcttgcaa aagctgctgt ctcacaggcg
      541 aatcgatacc tcatcgaaaa agaagggctt tctgtcatcg atttcgatca gattcaaaag
      601 aacaaaaaag atcagataag cgcttatcag gcagagacag gtggctctat cgatattatc
      661 acttatattg cacaaaaact aaatgcggac atctaccttg aaatagatgc taagacaagc
      721 tttggaggag tacctggagc ttggactgga tcagcacaag gtagcatcaa gatttttgat
      781 gcgtcgacgg cagccctcct cggatcgatt tcctttttaa gcccacagac atttagccct
      841 gtatctccag atgcagcgat gatgaatgct atatcaggga ttatctggca atcgatgcca
      901 aaggtaacag aacaagcaaa acatcttatg agcaccctga ccgcagctcg aggagttcga
      961 tacgaaatta ttcttcagaa tactcctgat gcaatggcgg tatctcaatt tgaaaggaat
     1021 ttagccaaaa gagtgcgcga ggttgaaaga ctgtcctatt cgccagggga aactcgcttt
     1081 gcactctatg cttttatgcc agcgtcaaag attcaagata ccatctatga tgttgcttcg
     1141 atgtcgggtt ttccaaattg ttatcttcta tatatgagag gaaggtccta cacattcaat
     1201 acgggaattt aacgtattta tataatgttc attattagga ggaaaagatg aaagctagga
     1261 aggtagtatt gatcgttcta gtcgccgcag tcgtaatctc atgcggtaca aagccccctc
     1321 ttcggaaggg agaagtgctt attgaggata aaggcactgc atacggcgtt aagacaccta
     1381 aatgggtaga gcttgcaatc gtaggtggct accgggatat tgaaaaacta ccagattata
     1441 aggataaagt agttttcata gctcaattcg aagctcagaa tctccaaagt gcacaattgc
     1501 tcgctgagcg tatgcaggct gatactgaga ttgctcgcta tctgtcgaca cgggtcaaag
     1561 atgcattcaa aggtgcgaat gtcgcagatg cggactccaa gaattttggt gcatatggag
     1621 aacgctttgt agcgagtgtg ggtgaagcta aattctctgg ctttagaatg gaggccgatt
     1681 ggtgggttaa agttcaaact tatactccag agaataagcc tgataagcaa atatatcggg
     1741 taattcagct atgggcgata gacaagaata tgttgcaaaa acagttcgat atactattct
     1801 ctcagatggc agggggcgag ccagcaacgc ctgagcagaa gcgtgctatg gatctggtcc
     1861 aaaacacagt agccaaagac ttctttggtg aatctcattg attcaaaaac gaattaatag
     1921 aaagcctcgg tttatgtatg taatgcgagg ctttctttga tttctactag attttatcac
     1981 cccgagagga attatgaatg aaaaagagca tcattatatg gatggtgatt tttttcttgc
     2041 ttgtggcaag ctgtgctagt gcgcccagca ttccggaatg ggtgaaaaag acaccaagcc
     2101 ctgattcaaa atatacttat tttgttgggt catcttcggc aaaagatagc gcaacagcac
     2161 ttaatgatgc aactgcagca ttgattgcgg gtattatgca gtacatggga gtatcgataa
     2221 gcgtagaatc tagcgccgaa gcaaaagctt ctctcagcga atatgaggca cagataacac
     2281 agacagtcaa gacagaatct aagggtcggc tttcgggttt tgaggtggtc gagaaatata
     2341 tacagagtga taaaaaaact gggctgtata ccgtgcatgt cctagctcga tatgaaacaa
     2401 aggagcttaa taaagagaaa gctcgaatcg aatccttact caaggaaaaa gccgatgccg
     2461 tggcaatacc agaacagaag ggtgattctg cagccaacga ggggcacttg atcgatgcca
     2521 ttcgtgctta tgcggaagct atgtcagcag caggaagttc tgatatagag aatgcacaga
     2581 taaaacttga acgcaatgct aagaaagctt ctgcgttagc cgctactctt aaactggtag
     2641 tattgtctcc atcaaatcaa gaaataccgc taggcagcac taatgctcca tcatttgtgg
     2701 tgaagcttat aactaatcgt agaggaatag aagaaggagt gcctggtgct ccccttgttg
     2761 taacctatcc tcgaagactg acttcaggac gaatgggtac tactacacag caagtcttta
     2821 ccaatcaaga tggcatcgtc tcatttgatg ttcctgttgt agaattagct ggggattatc
     2881 aggtaaccgt gcagctagat ttcggaagta ttgctgatat tttaggaggc ttacctggat
     2941 gggcattgcc atacatggaa gcattagaaa gtgatttgtc aggcataaga gtatatatga
     3001 gttataaggt tgtctcagcg gctaaaagtg ttcctacggc gattggggtc tatctcaaag
     3061 accaaacaca gaaaggtagc ctagatcccg aaacatttgt aaagagtctt gaagaagctc
     3121 tcgtaaagga aggtttctct attttcacga caaaaatctc cgctgaatat ggaaagcccg
     3181 atattgcgca gctcaaagcc attgccccaa ctcaggctaa gcgctttatt ttggccactg
     3241 ttgagcttgt ctcgattaat aaagaggata gttttttcat cgcttcggtc acaggatctt
     3301 tgagtgcaat tgatttaata gaagggaata ctctctattc ggggagcaag ggcgctcaag
     3361 gaatggggtt ttctgaggca gaggcgattt ctgcagcact caggacactc ggtggtcaag
     3421 tgtttgccaa ggaactattg gctgctttgc gctgagaatt aacctaagat tgattataaa
     3481 tttaaaccaa agaatcatag tggcacaact atcgaagtcc cactttct
//
LOCUS       DAOSOB010000101         3514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_26779_length_3514_cov_66.020237, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000101 DAOSOB010000000
VERSION     DAOSOB010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26779_length_3514_cov_66.020237"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            459..1814
                     /locus_tag="PLS88_08160"
     CDS             459..1814
                     /locus_tag="PLS88_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HOU07357.1"
                     /translation="MKLSQWNGDLGGIDYVSLIVIDIDGRMRAVSLPSSFADEAVLKK
                     GIGFDASNFGYAKVHASDMVAVPDMDTAFVEQKDGLDILHVFCDVRTTEGELFEQYPR
                     SVIRAAHAALMESGIGNDIKMLVELEFYVFEEVHYSTATDHSYYFVESSEGIGEEYSD
                     TPWIGLSQGYHRMAPEDRYQLLRNRAVKTMIDVGIPVKYHHHEVVAAQLEIELDFISM
                     VKAGDAVSLAKWILRNEADELGLKITFMPKPMYKVAGSGMHVHQFIEKAEASIFPGEG
                     LYGLSGEGLSYTAGLLSHALTGSLLAFSNPSTNSYRRLVPGYEAPVSATFAQGSRAAA
                     VRIPGYLRKGEARIEFRTGDATANVYYFLAAMLLAGLDGIKKELDPVALGFASETTSA
                     QHVFPTGLFNVLTGLKKDHDYLSSAFSEELIETWIARKEKEASYVYNAPVPQEYELYF
                     D"
     gene            1902..>3514
                     /locus_tag="PLS88_08165"
     CDS             1902..>3514
                     /locus_tag="PLS88_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU07358.1"
                     /translation="MSQKVLLSGDEAIAQAALDSGIMAAFAYPGTPATEIMEYLQENI
                     GDASNDESNNATEFRPFAQWCTNEKTAYESALGVSFVGGRTMVSMKHVGLNVAMDPFV
                     NSALVRINGGLVVVVADDPSMHSSQNEQDSRLLADFAHVPCFEPSDQQEAYDMVRAAF
                     DYSETHEVPVMVRIVTRLAHARALVQTATPVPIANRGKTADRLSWTLLPALARKQWSA
                     LLGKQQAFRADGEKASRLALRSKELGVVTCGLALAYYKENEEDWAVAHGGERPSHLHL
                     SRYPIEDLEGESRIRVLAAHVSRILVIEEGYPYIEGKIRGIFGAPISISGKMTGELPI
                     AGELSADSVRLALGLPAVSRTLHAEIELPGRPPQFCQGCPHADSYTALKKALEDEAEF
                     FVASDIGCYALGAQPPWNAIESCVDMGASVGMARGAGCVGYKRSIAVIGDSTFYHSGM
                     TNLLDAVRHHSPMTLIILDNGTTGMTGAQPTISPSQQLPKLLEGLGVEKEHIRVLEAH
                     RRALDSNVATIREELCYDGVSVIIMFREC"
BASE COUNT          864 a          771 c          945 g          934 t
ORIGIN      
        1 agcaatccat atatttatct gtggtagatc tcttccatcc cccatctacc caatagcgaa
       61 gttccttgat ctctgacatg ccgttttgct cctttctact cgtattcttg tagcaaatta
      121 ttgcaaattt gatataaata gatatcgaat gtcaaggctt agtcattctc ggcttctatt
      181 gcttagcaga tagactggct taatctgacc taatctgact cgattatttg gaaatctttg
      241 cattgtcttt tgaagccgtt tcattctttg ataccatcgt ttcataactt gccggatgaa
      301 tgatctcggt ttacaatata ttgatgcagg aagatcatcc taaaatctgt tttgatttgt
      361 agactcagcg aggtcaaaga taaaaagaaa tagcccaaga aggatggcac gatgagtcgc
      421 tcactaagcc gcgctcgcgg cacagcgagg agttttatat gaaattgtct caatggaatg
      481 gtgatttggg gggtatcgac tatgtatcgc tcatcgtaat cgatatagat ggacgaatga
      541 gggcggtaag tcttccttct tcatttgctg atgaagcggt actcaagaaa gggatcggat
      601 tcgatgcctc caattttgga tatgcaaaag tgcatgcatc agacatggta gctgtccccg
      661 atatggatac ggcctttgtg gaacagaagg atggattaga tatactccat gtattctgcg
      721 atgtacgtac aacggaaggc gaattgttcg agcagtatcc gcgttcggta atcagggctg
      781 ctcatgcggc tctgatggaa tctggaatag gcaatgatat caagatgctc gtagagcttg
      841 aattctatgt tttcgaagaa gtgcactatt cgacagctac ggatcattcc tactacttcg
      901 tcgagagttc ggagggcatt ggcgaggagt atagcgatac gccatggatt gggctttccc
      961 aagggtatca tcgtatggcg ccggaggata ggtatcagct tttgcgaaat cgtgcggtaa
     1021 agacgatgat cgatgtcggc attcctgtca aataccatca ccacgaagtg gttgccgcac
     1081 aactcgagat cgagcttgat tttatatcga tggtgaaggc gggagatgcc gtatctctgg
     1141 ccaaatggat tctccgtaac gaggccgatg agcttggact gaagattact tttatgccca
     1201 aacctatgta taaggttgcg ggtagcggca tgcatgtaca tcaattcata gaaaaggcgg
     1261 aggcttcgat tttccccggt gaggggttat atggactttc aggggaaggt ctttcgtaca
     1321 cagcggggct cttgagtcac gcgcttacgg gctcactttt ggctttttcc aatccttcca
     1381 caaattcata tcgaaggctc gtgccaggct atgaggctcc ggtgagtgca acttttgcac
     1441 agggatcgag ggctgcggca gtgcgaattc ccggttatct aaggaaagga gaagcgcgca
     1501 tagagttccg cacaggggat gctactgcaa atgtgtacta ttttttggca gccatgcttc
     1561 tagctggtct cgatgggata aagaaagagc tcgatcctgt agctcttgga tttgctagcg
     1621 agacaacttc ggcgcagcat gttttcccga ccgggctttt caatgtgttg actggcctca
     1681 aaaaggatca tgattatctt tcttctgcgt tttcagaaga gctcatagaa acctggatag
     1741 cacgcaagga aaaagaagcc tcatatgtgt ataacgctcc tgttccacag gagtatgaac
     1801 tatactttga ttgaatagat ggattttcag cgcaggtttg ctcctgcgct tttttttggt
     1861 tttttgattg ctaattggcc ggaggagtaa ggagttcaga aatgtcacaa aaggtccttc
     1921 tttcgggaga tgaagcgata gcacaggctg cgctggattc gggaatcatg gctgcttttg
     1981 cttatcctgg gacgcccgca acagaaataa tggagtattt gcaggaaaat ataggcgacg
     2041 caagcaatga cgaaagcaat aacgcgacag aattccgacc tttcgcacag tggtgcacta
     2101 atgagaagac agcttacgag tccgcgctag gcgtgtcttt cgtaggtggg cgcacgatgg
     2161 tttcgatgaa acatgtcggg ctaaatgtgg cgatggatcc ttttgttaat tctgcgctcg
     2221 ttaggatcaa tggggggctc gtagtagtcg tagcggacga tcctagcatg cattccagcc
     2281 aaaacgagca ggactcgagg cttttggcgg attttgctca tgttccttgt ttcgaaccgt
     2341 cggatcagca ggaagcttac gatatggtaa gagccgcatt cgattattcg gagacacatg
     2401 aggtgccggt aatggtgcgc atagtcacaa ggcttgcaca tgcacgcgcc ctggtgcaga
     2461 cagcgactcc agtgcccatt gccaatcggg gcaagaccgc cgaccgttta tcctggacgc
     2521 tcttacctgc cctggcgcga aagcagtggt cggccttgct cggaaagcag caagcattca
     2581 gggcggacgg agaaaaagcg agccgccttg cgctgcgatc gaaagagctt ggtgtggtga
     2641 cctgtgggct ggctctggcg tactataagg aaaacgagga ggactgggcg gtggcgcatg
     2701 gcggcgagcg gccttcgcac ctacacttaa gccgttatcc gatagaagat ctagaaggtg
     2761 aatctaggat cagagtcctt gctgcgcatg tttcgcggat tttggtaatc gaagagggct
     2821 acccttatat cgaaggcaaa atacgtggca tatttggcgc tcctatatct atttcgggga
     2881 aaatgacggg agaattgccg atagcaggcg agttgagcgc agattctgtg cgactagcac
     2941 ttggacttcc tgccgtttct cgcactctgc acgccgaaat cgagcttcca ggtcgtccac
     3001 ctcaattctg tcagggttgt ccacatgccg attcctatac tgcactcaaa aaggccctcg
     3061 aagatgaagc tgaattcttt gtcgcttcgg acattggctg ctatgctctc ggagcacagc
     3121 caccttggaa tgccatagag tcctgtgtgg acatgggcgc ttcggtgggg atggcgcgtg
     3181 gtgcaggctg tgttggatat aagcgctcca ttgcggtaat aggagattca accttttatc
     3241 attcgggtat gacaaatctg ctcgatgcag tgcgacacca tagtcctatg accctgatta
     3301 ttttggacaa tgggactaca ggcatgaccg gagctcagcc cacaatcagt ccaagccaac
     3361 aactgccaaa gttgctcgaa gggcttggtg tcgaaaaaga gcatatccgt gtactggaag
     3421 cgcatcgccg tgcattagat tccaatgtcg cgacaattcg cgaagagctt tgctacgatg
     3481 gtgtctcggt catcataatg ttccgggaat gcat
//
LOCUS       DAOSOB010000102         3464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_27272_length_3464_cov_70.304488, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000102 DAOSOB010000000
VERSION     DAOSOB010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27272_length_3464_cov_70.304488"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLS88_08170"
     CDS             complement(<1..701)
                     /locus_tag="PLS88_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOU07359.1"
                     /translation="MQGLAKEESEELNEIARELRCTSLRMIHRRQAGHPGGSLSAAEI
                     MTVLYFKVMRIDPENPNWDERDRFLLSKGHASAMFYATLAKRGFFPEEELQKWGELDC
                     HLQGHPDRLKTTGVDMNSGILGHGVPIGVGLCLAARHKRKDYRVFTLLGDGECQAGLV
                     WEGANAAAKYALTNLVAIIDHNGVQLDGFVEEVMPIEPLAEKWRSFGWDVLEVDGHDV
                     DQLYQAFLQCIEAQK"
     gene            complement(720..2699)
                     /locus_tag="PLS88_08175"
     CDS             complement(720..2699)
                     /locus_tag="PLS88_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU07360.1"
                     /translation="MEYKEIEKARIERANILAVFDGLDDALAANRIPLHIDATLSELL
                     ILGMLLQNVKTYFTVFGHGSTEIGEVLRIFEKAGLVRVLGLRNEIEASHAATALNWIT
                     HEKAAVVTSIGPGAFQALAASLVPASDGIGVWYLFGDETTEDEGYNMQQIPKNQEFLF
                     PQVLQAMGSAYSLQDPWALSTALRRGHARVDHPFRPGPFYVSMPMNVQSRMLKGFNLR
                     ELQCGEISCIGAAVGDYEKAVAWIKDSRSAVIKIGAGAKGATEEILQLAELIDGVCVY
                     TPIAGGIVPYEHPRCMGVGGSKGSICGNYAMEMADLLIAIGTRAVCQSDSSRTGYPNV
                     RKVININADLDDALHYQDSLALMGDAKLTLKELIGALKRASEEGLKKALREEPEEALK
                     EALKEKSEEVSRESYGQAHTESSDWLFACAAKKSEWEAFKQERFDNPTLEDSFWRRRV
                     LTQPAAIKIALDWSADKEVINIFDAGDVQANGFQIASDEWSGKTITDSGASYMGFAVS
                     AILASAAKPDCPYILAFSGDGSFTMNPQILIDGAEHGARGCILLFDNNRMAAISGLQM
                     AQYGNEFATWHEKPIDYLGWAKSVPGVMAISGGHSPEELRRALDKAFDYKGLSLIHIP
                     VYYSTDPLGSMGVYGRWNVGNWVEETQKLRHKIGL"
     gene            complement(2666..>3464)
                     /locus_tag="PLS88_08180"
     CDS             complement(2666..>3464)
                     /locus_tag="PLS88_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOU07361.1"
                     /translation="NKSMAEIQSLARGLKILEIMAESEFGIGVTELAEQLQVDKSTAS
                     RLVQTLVKDGFVQRSEDGRTYTLGPTLVNLSRSVIIRMPLREAAKPFLRELVELSGEC
                     AHLAIYAQGKALYIDQMESPSTLRVNVEVGHMAPLHCTALGKALLAFGDYPIPYELEG
                     YTSKTIKDPKILQRELQQVKEQGYAIDDEEFDPDVRCIASPVFDYRDKLVGAIGISGP
                     STRLSLPRIKVLAPQIKNIAERLSDRLGFNEDKRTAKWSTKKSRKRE"
BASE COUNT          868 a          941 c          689 g          966 t
ORIGIN      
        1 ttcttctggg cttcaatgca ttggagaaaa gcctgatata gttgatcgac atcatgccca
       61 tccacctcga ggacatccca tccaaacgat ctccattttt ctgcgagcgg ttcgattggc
      121 ataacttctt cgacaaatcc gtccagctgt actccattgt ggtcgataat ggcgactaaa
      181 ttggtgagag catatttcgc ggcagcattt gctccttccc agacgagccc agcctggcat
      241 tcaccatctc ctaggagggt aaaaacccga tagtcttttc tcttatgtct tgctgcaagg
      301 catagcccta ctccaatcgg aacgccgtgc cccaaaattc cgctattcat atcgacaccg
      361 gtcgtcttga ggcgatcagg atgcccttga agatgacaat ccagttctcc ccacttctgt
      421 aattcttctt cagggaaaaa accacgcttt gcaagagtgg catagaacat tgccgatgca
      481 tgtcccttgc tcagcaaaaa ccgatctctc tcatcccagt ttggattctc aggatcgatt
      541 ctcataacct tgaaatataa aacagtcatg atttctgcgg ctgatagtga tccccccgga
      601 tgcccagcct gccttctatg tatcattcgt agacttgtgc atcgcaactc acgggcaatc
      661 tcgttcaatt cttcgctctc ttctttagct aagccttgca tttcgcaatc ctatcctatc
      721 tacaatccta tcttgtggcg caatttctgg gtctcttcga cccaattgcc aacattccat
      781 cgcccataga ctcccataga gccaagggga tccgtactgt aatagactgg aatatgaatg
      841 agagataatc ctttgtaatc gaaagcttta tccaaagctc ttctcagctc ttctggtgaa
      901 tgacctccgc ttatggccat tactccgggc acggattttg cccatcctag gtaatcgata
      961 ggcttttcgt gccaagttgc aaattcgttt ccatattgag ccatctgcaa accagaaata
     1021 gcagccatgc gattattgtc gaataaaaga atgcagcccc ttgcgccatg ctctgctcca
     1081 tcgatcaaga tctgaggatt catcgtaaag gagccatcgc cgctaaaagc cagaatatag
     1141 ggacaatctg gcttcgccgc gcttgcaagg atcgctgaaa cagcaaatcc catataactt
     1201 gctccggaat ccgtaatagt cttacccgac cattcgtccg aggcgatctg aaaaccattc
     1261 gcctgcacat cgcccgcatc gaaaatattt atgacttctt tgtccgctga ccaatccaat
     1321 gcaattttga ttgcggccgg ctgggtcaat actcttcttc tccagaaaga atcctcgagc
     1381 gttggattat caaagcgctc ttgcttgaag gcttcccatt cggacttttt agctgcgcaa
     1441 gcaaacagcc aatccgacga ttcggtatga gcctgtccat aagattctct agatacttct
     1501 tcagactttt ctttcaatgc ctctttcagc gcttcttcag gctcctctct cagcgctttt
     1561 ttcaggcctt cttccgatgc ccttttcaat gcgccgatca gttctttcag cgtgagtttt
     1621 gcatctccca tcaacgcgag cgaatcctga taatgaagag catcatccag atccgcattg
     1681 atatttatca cttttctgac atttggatat ccagtgcgcg agctatccga ctgacagact
     1741 gctctggtac caatagcgat aagaagatcg gccatttcca tggcataatt gccacagata
     1801 ctccctttcg atccgcctac ccccatacag cgtggatgct cgtaaggcac tatgccacct
     1861 gctattggag tgtaaacaca tactccgtct attaattccg caagttgcag aatttcttct
     1921 gttgctcctt ttgcacccgc gccgatcttt atcacagccg atcttgaatc ttttatccaa
     1981 gcgactgctt tttcataatc tccaacagca gctcctatac aagatatttc tccgcactgc
     2041 agctctcgca gattgaaacc ctttagcata cgtgattgca cattcatagg catggacaca
     2101 tagaaagggc cagggcgaaa gggatgatcc actctcgcat gccctcttcg gagcgccgtc
     2161 gaaagcgccc aaggatcttg gaggctatag gcagagccca tcgcctgcag aacctgagga
     2221 aatagaaact cctgattctt aggaatctgc tgcatattgt agccttcatc ctcggtggtc
     2281 tcatccccaa acaggtacca tacacctatg ccatcggaag cgggcacaag cgaagcggct
     2341 aaagcctgaa aggcaccagg cccaatggaa gtgactacag cagccttttc atgtgtaatc
     2401 caattgagag ctgtagctgc atgggaagct tcgatttcat tccgtaaacc caatactctt
     2461 accagcccag ccttctcaaa gatgcgcaaa acttcgccaa tctccgtcga tccatgacca
     2521 aacacggtga agtatgtttt tacattctgc agaagcatgc ctagaatcag cagttctgag
     2581 agcgttgcat ctatatggag cggaattcta ttcgctgcca atgcatcgtc gagcccatca
     2641 aagacagcaa ggatgttggc tctctctatt ctcgctttct cgatttcttt gtactccatt
     2701 tagcagtcct tttatcttcg ttgaatccca atctatcgga aagtcgctca gcgatattct
     2761 tgatctgtgg agctaatacc ttgattcgag gaagactcaa acgggtagaa ggaccagaaa
     2821 tgccaatagc ccccaccagt ttatctctat aatcaaaaac cggagaagca atacagcgca
     2881 catctggatc gaattcctca tcatctatcg catatccctg ctcttttacc tgctggagct
     2941 ctctctgcaa gattttggga tctttgattg tcttcgaagt atagccctct aattcatatg
     3001 gaattggata atctccaaaa gccaatagtg ctttccccaa agcagtacaa tgcagcggag
     3061 ccatgtgtcc gacctctaca tttaccctta gcgtagaggg cgattccatc tgatctatat
     3121 agagcgcttt cccttgggca tagatagcca agtgcgcaca ttctcctgac agctccacca
     3181 attctctcag gaatggcttc gcagcttctc tcaaaggcat gcggataatg acgctgcggc
     3241 ttaagtttac aagtgttggt cctagtgtat atgtcctacc gtcctctgat ctttgaacaa
     3301 aaccgtcttt tactagggtt tgaacaagcc ttgaagcagt ggatttgtcc acctgcagtt
     3361 gctcagctaa ttctgtaaca ccgattccaa attcgctttc agccataatt tctaggatct
     3421 ttaagccacg agccaacgat tgtatttcag ccatagattt attc
//
LOCUS       DAOSOB010000103         3285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_29236_length_3285_cov_67.444892, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000103 DAOSOB010000000
VERSION     DAOSOB010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29236_length_3285_cov_67.444892"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..1516
                     /locus_tag="PLS88_08185"
     CDS             329..1516
                     /locus_tag="PLS88_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HOU07362.1"
                     /translation="MKKDNSMEEKKSFPYFKVICLVIALIFAFFGGREFYELRHFKET
                     FVSAPGLTEVKMLSDYLGSLKGTWMDTPIFVYDSGVPGGKVLYMANVHPYEPATSLSA
                     YILMENIKVTKGKVFVIPQGNRSGSTTGMLGNAYPKFFHVPTSFGEKTLRIGDRGTNP
                     LDQWPDPFTFVHYPSRQNLSYQDIRNMNRTYPGRENGTLTERASYAIMELIRKENIDI
                     SIDVHEASIMYPVVATYVAHERSFDIAMMAAMTLTATSFDMKCESSPKGLRGLSHREW
                     GDFSDTLAVLMETPEPFIDRVVGPMTEELFTEGKDEFLATAAKHGLTYVPYSLDFGSP
                     LWYRCGRHLSGALEVINQMNQFYPEKEVIVEWPTYEDLKEHDCGYFFHDPDNADPSFV
                     FKI"
     gene            1661..3265
                     /locus_tag="PLS88_08190"
     CDS             1661..3265
                     /locus_tag="PLS88_08190"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOU07363.1"
                     /translation="MKKFVMLLTIALFVFSAQSSFACTIVGVGADATVDGSTIVTHND
                     DSTSADFRLWIIPGQKWPEGSTRDLVIDSHNYGDFGKYPEVKDYGNGMLVSEIPQAPE
                     TYAYFHSRYSFINEKGVAMGESTFNFDQSTDLRKKTYKLIFGNNTGLIDCWNAQDIAL
                     ERASTAREAIQIMGDLVEKYGWKDPGETMDVADGKEVWIAEFYGLDLWAAVRIPSNAF
                     FVAANRARIDHIDFNDHENYMYSPNIKSFAVENGLWSEDSGVPFSPAEIYAPYEGTYS
                     TRREWRALSLVAPSLNLDPNAKRFPLWVIPEKKLSVQDVLEICGDYYKGTPYDLSLAP
                     EAGPYGDVLNPYHKERSINLFRTCYVMIANIKDRLPDPVKCLVWYGYGAADTTYITPL
                     WPTMKELPEFYRTGSRFEEFRRDSGWWTNSYVQQTARINYQSAVKEIHDFRNPRLATL
                     YTVTEEIQNTAASLINNGKEKEAADLISSFAYTTAVKWHDDWLKFGDELYGTYMWGYK
                     DMKSQPPSAWWNEIMQKAPRNPVPFK"
BASE COUNT          943 a          682 c          756 g          904 t
ORIGIN      
        1 tccatcgaga cagtgggtta caaaggctct tatatgtctt tcctaaaaca gatattgctg
       61 cctttgcttg tgcttggagg tttggcactc atcatgctga ttattcctaa taagctcagc
      121 ttccttaaat gaggagaaac atatggtttt tcttgaacaa gtaatacata taatctactt
      181 tatcctagcc gctttaatag gttttttcct tttgaggaat ttattcaaga ggacatcgcg
      241 aactggccga gtgtacgaca ttgtatatgc ttattgcatt attccctttc tcttgcgcgt
      301 gttaggcatc aaataagagg agggagctat gaaaaaggat aatagcatgg aggagaagaa
      361 atcttttcca tattttaaag taatatgtct tgtaatcgca ctgatattcg ccttcttcgg
      421 aggaagagaa ttttacgaac tccgacactt caaagagacc tttgtttcgg ctcctggttt
      481 gaccgaggta aagatgctaa gcgattacct tgggagccta aaaggaacgt ggatggatac
      541 tccgatattc gtctacgatt caggtgttcc tggaggcaag gttctctaca tggccaatgt
      601 ccatccttat gaaccagcga caagtctctc tgcctatatt ttgatggaga atattaaggt
      661 aacgaagggg aaagtatttg taataccaca gggtaaccga agcggatcga ccacaggcat
      721 gttggggaat gcctatccta agttttttca tgttcctacg tcttttggag agaaaacgct
      781 gcgaattgga gatcgtggaa ctaatcctct cgaccaatgg cctgatcctt tcacatttgt
      841 ccactatcca tcgaggcaga acttgtccta tcaggatatt cgaaacatga atcgaaccta
      901 tccaggaagg gaaaatggta ctctcacaga gcgagcttcc tatgcaataa tggagcttat
      961 ccgcaaagag aatatcgaca tatcaataga cgttcatgaa gcatcgatca tgtatcctgt
     1021 ggtagctacc tatgtggcgc acgagcgttc atttgacatt gcaatgatgg ccgcaatgac
     1081 acttacagct actagtttcg atatgaaatg cgagtcttct ccaaaaggcc ttcgtggatt
     1141 gtctcaccgc gaatggggcg atttcagcga tactcttgct gtgcttatgg agactccaga
     1201 gccttttatc gatcgtgtgg taggacctat gacagaggag ctcttcacag aaggcaagga
     1261 tgagtttcta gccactgcag cgaagcacgg actcacctat gtaccatata gccttgattt
     1321 tggctctcct ctttggtatc gctgcggacg acacctttca ggagctcttg aagtaataaa
     1381 tcagatgaat cagttctatc cagaaaagga agtgatagtt gagtggccga cctatgaaga
     1441 tctcaaagag catgattgtg gatacttctt ccatgatcct gataatgcag accccagttt
     1501 tgtattcaag atatgagcaa ttaagaaaac agaaaaatta catgggccgg aaatatctga
     1561 aatttgaatc tatgccgccg ctaacctaag atagagtatt ttatcaaaag gtaagcggag
     1621 acaagagaat atggggtatt ccccaagaaa ggagtgcgat atgaagaaat ttgtaatgct
     1681 gctgacgata gccctattcg tatttagtgc acagtcatcg tttgcatgca ctatcgttgg
     1741 cgttggcgca gatgcgaccg tcgatggctc gacaatagtc actcacaacg acgattctac
     1801 atcggcagac tttcgccttt ggataattcc agggcaaaaa tggccagaag ggtcgactcg
     1861 agatcttgtc attgactcgc ataattatgg agatttcggc aagtatcccg aagtgaagga
     1921 ttatgggaat ggcatgctag tctcagaaat tccacaagct cctgaaactt acgcctattt
     1981 ccattctcgc tattcattta tcaacgagaa aggtgtggca atgggcgaga gcaccttcaa
     2041 tttcgatcaa tctacggatc ttcgcaagaa gacctataag ctaatctttg gaaacaatac
     2101 aggattgatc gattgttgga atgctcagga tattgctcta gagcgagcat ctaccgctag
     2161 agaagccatt caaataatgg gagacctggt agagaaatac ggatggaaag accctggcga
     2221 aacaatggat gtagctgatg gcaaggaagt atggattgct gaattctacg gactagacct
     2281 ttgggccgcg gttcgcattc cgtccaatgc tttctttgtt gctgcaaata gggctcgcat
     2341 cgaccacata gatttcaatg accatgaaaa ctatatgtac tctccgaaca ttaagagctt
     2401 tgctgtagaa aatggtctct ggtctgaaga ttctggagtt ccattctctc ctgctgagat
     2461 atatgcacct tacgaaggta cttatagcac ccgccgcgag tggagagctt taagccttgt
     2521 tgctccttct ttgaatctag acccaaatgc gaaacgcttt cctctctggg tcattccaga
     2581 aaaaaagctt tcagtacaag atgtattaga gatttgcggt gactactata agggaacgcc
     2641 ttatgatctt tctctagctc ctgaggctgg tccttatggg gatgtcctta acccctacca
     2701 taaggagagg tcgatcaacc tctttagaac ctgctatgta atgattgcaa acataaaaga
     2761 tcggctacct gacccagtga aatgtcttgt atggtatgga tatggagcag cggatactac
     2821 ttacatcacg ccgctctggc caaccatgaa agaattgccc gaattctata ggactggaag
     2881 tcgctttgaa gaatttcgta gagactcggg ctggtggaca aactcttatg ttcaacagac
     2941 agcaaggatc aactaccaga gcgccgtaaa agaaattcac gatttccgca atccaagact
     3001 tgctactctg tatacagtta ccgaagaaat acagaataca gctgctagct tgatcaacaa
     3061 tggaaaagag aaggaagcgg ccgatcttat ttcttccttt gcttatacta ctgcagtgaa
     3121 atggcacgat gattggctaa agtttggcga tgagttgtat ggaacttata tgtggggcta
     3181 taaagatatg aagtctcagc caccgagcgc ttggtggaac gagataatgc agaaagctcc
     3241 aagaaaccct gttccattca agtgatagct tggtagaata ggcac
//
LOCUS       DAOSOB010000104         3003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_32818_length_3003_cov_51.568860, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000104 DAOSOB010000000
VERSION     DAOSOB010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32818_length_3003_cov_51.568860"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLS88_08195"
     CDS             <1..782
                     /locus_tag="PLS88_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU07364.1"
                     /translation="IYGVYLSFFDWNILSSKVFIGFKNYVEAFRDPEFLSSFSHTLQF
                     VCISTPLIIIVGFVMALITIQPTRTGRVAETVFFLPYMLSTSVVGTLWAWIFQKNYGL
                     ANQVLKLLNLKPLGWLTDPSIAMTSIIIATLWWTAGFNMILFSAGMKQIPDEIYDAAK
                     IDGAGSFTTLTKITLPLLKDTSLLVLILQVIASFKVFGQVYVMTGGGPYGTTRVLVQY
                     VYKTGFNYFRLGYASAMSVILFLVILLISSLQFLRKPDQAQ"
     gene            779..1594
                     /locus_tag="PLS88_08200"
     CDS             779..1594
                     /locus_tag="PLS88_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOU07365.1"
                     /translation="MRKLLNILVKVLILCLIIIWLIPVIWLLVTSLKSENTVMTDKLS
                     LIFTPTLENYKKAFSSTLLGRWLVNSLIVSVITTILTLLIDSTMAYVLARIQFRGRNV
                     LFIFILAGMMIPFEVLIIQLYLELTKMGLANTLIAVMLPRLAMPIGVFILTQFFKSIP
                     IVLEEAAFIDGASRFTVFRTIIMPLSKSALFAVAIISFINAWNDFLWPLVAISSSEKY
                     TITVGIANFQGTHGTEYSLIMAGAVLASIPQIIMYILFKKNIVKSIAMTGIKE"
     gene            1598..>3003
                     /locus_tag="PLS88_08205"
     CDS             1598..>3003
                     /locus_tag="PLS88_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU07366.1"
                     /translation="MNRAVDFSSIGNNSIFEDHPNPSWARRTWISLDGKWTIEHKREK
                     SSIQVPYPVGSQLSGVHFLDKGTFKYSKSLEIKELDKKKRFILNVGACDYLTKVFVNS
                     SEVGRHVGGYSSFAFDITEALHKGTNKIELIVRDSHSPFQVRGKQTFLRKPFYVWYKG
                     ISGLWQNIWMEIVDPVYLRHGELNPDFDANIFRGKIRTSLLDNDSPENLEIADYSLNI
                     EIVSPSGEIIDLQPIKMNNAGEFHFQCSFSEIKATLWSPDNPALYRIRYDLYREQQCI
                     DTVESYFGLRSVHIDSDGSFLINGKKCFIKMALAQGYYPKGVYTPENKKIIQNDILNL
                     KLMGYNGARIHEKIESPYFQYLCDKIGIFTSFEMPSFYLPSRKGFRAYEQELRELIER
                     DAGHPSCIFRILFNETWGIWGIYKNKSRTDKFVRKMIVMTRKLDPSRPIIDNSGWEHI
                     DTDIVDFHHYLGSASLAR"
BASE COUNT          926 a          606 c          588 g          883 t
ORIGIN      
        1 taatatacgg agtctatcta agtttctttg actggaacat tctatcctca aaagtattta
       61 tcggattcaa aaactatgtc gaagcattca gagatccaga attcctttca tctttttctc
      121 atactcttca atttgtttgc atttcgactc ctttaataat aatcgtcggt tttgtaatgg
      181 cacttatcac aatccaaccc actcgcactg gaagggttgc tgaaactgta ttctttctgc
      241 cttatatgct ttctacgtct gtagtgggga ctctttgggc atggatattt cagaagaatt
      301 atggccttgc gaatcaagta ttgaaattgc ttaaccttaa acctcttgga tggctcacag
      361 atccgtccat cgctatgaca tcgataatca ttgcgacatt gtggtggaca gcgggattca
      421 atatgatctt gttttccgca gggatgaagc aaataccaga tgagatatac gatgcggcaa
      481 aaatcgacgg tgctggaagc tttaccacat tgacgaaaat aacgcttcct cttctcaaag
      541 atacttcact gctagttttg attctccaag tcattgcttc atttaaagtt tttggacagg
      601 tgtacgtaat gaccggcgga ggtccatacg gcactacgag agttcttgtt cagtatgtat
      661 acaaaacagg cttcaattat ttcaggctag gatatgcttc ggctatgtcg gtcattctat
      721 tcttggttat tctgcttatc tcttctctcc aatttctaag aaaaccggat caagcacaat
      781 gagaaaacta ttgaacatcc ttgtaaaagt actcatccta tgtttaataa taatctggct
      841 cattccggta atttggctct tagtcacttc gcttaaatct gaaaacacgg taatgacaga
      901 taagctttct ttgatattca caccaacact tgaaaactac aaaaaagcat tttctagcac
      961 tctgctcggt agatggctag tcaattcgct cattgtttct gttataacta caatcttgac
     1021 attgctaatc gactccacaa tggcctatgt gcttgctagg atacaattcc gcggaagaaa
     1081 tgtattgttt atcttcattc tggctggaat gatgattcca ttcgaagtac tcataataca
     1141 gctatacctc gagctcacaa aaatggggct agcaaacact cttattgccg taatgttacc
     1201 acgccttgcc atgcccattg gtgtattcat acttacacag ttctttaaat ctattcctat
     1261 tgtcctcgaa gaagcagcat tcatcgacgg agcaagcaga tttaccgtat tcagaacgat
     1321 tattatgcca cttagtaaat cagcattatt cgcagttgcg ataatatcct tcataaatgc
     1381 atggaacgat tttctatggc cactagttgc aatttccagt tcggaaaaat acacaataac
     1441 agtgggaatc gcaaacttcc aagggacaca tggcaccgaa tattcgctca taatggcagg
     1501 agccgtactt gcgtcgattc ctcagataat tatgtatatt ctttttaaga aaaacatcgt
     1561 aaaaagcatc gctatgactg gaataaagga atgaatcatg aatcgggccg tagatttctc
     1621 atccataggg aacaattcga ttttcgaaga tcatcccaac ccctcctggg cacgccgaac
     1681 atggatttct ctcgatggaa aatggactat agaacacaaa agggaaaaaa gctcaattca
     1741 ggtaccctat ccagtcggaa gtcaattgtc tggcgtacat ttcctagaca agggaacctt
     1801 taaatactct aaatcgctag aaatcaaaga gctggacaaa aagaaaagat tcattctcaa
     1861 tgtcggtgca tgcgactatt tgacgaaagt atttgtcaat agtagcgaag tgggaagaca
     1921 tgtcggaggc tattcatcat ttgcctttga tatcacagaa gcccttcata aaggcacaaa
     1981 taaaatagag ttgatagtac gagactccca tagccccttc caagttagag gaaagcagac
     2041 atttcttaga aagcctttct atgtgtggta taaaggaata tctgggttat ggcagaatat
     2101 ttggatggaa attgtagacc ctgtatatct taggcatgga gaattgaatc ctgatttcga
     2161 tgcaaatatt ttcagaggaa aaatacggac ttcgctgcta gataatgatt ccccagaaaa
     2221 ccttgagatt gccgattatt ctcttaacat cgaaatagtc tcacctagtg gcgaaattat
     2281 cgaccttcag ccgatcaaaa tgaacaatgc aggagagttt cattttcagt gttctttttc
     2341 ggagataaaa gccacattat ggtcgccgga taatccagcc ctttaccgaa tccgatatga
     2401 tttgtataga gagcaacaat gcatcgatac agtcgagagc tattttggcc tgcgaagcgt
     2461 acatatcgac agcgatggtt cttttcttat aaatggtaag aagtgcttta taaagatggc
     2521 gctcgctcaa gggtattatc caaaaggagt atatacacca gagaataaga aaatcattca
     2581 aaacgacatc cttaatctca aattgatggg ctataatgga gcgcgaatcc atgaaaaaat
     2641 cgaaagtcca tattttcagt atttgtgcga taaaattgga atattcacga gtttcgagat
     2701 gccatcgttc tatttgccgt ccagaaaggg atttagggca tatgagcagg aattgagaga
     2761 gcttatcgaa agagatgcag gacacccttc ttgtatcttc agaatcctgt tcaacgaaac
     2821 atggggaatc tggggaatct acaagaataa atccagaacc gataaatttg taagaaaaat
     2881 gattgtgatg acgagaaaac tcgatccttc tcgtcctata atcgataaca gcggatggga
     2941 acacatcgat acagatatag tcgatttcca ccattattta ggcagcgcat ctcttgcgcg
     3001 aat
//
LOCUS       DAOSOB010000105         3001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_32845_length_3001_cov_78.963340, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000105 DAOSOB010000000
VERSION     DAOSOB010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32845_length_3001_cov_78.963340"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLS88_08210"
     CDS             <1..300
                     /locus_tag="PLS88_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU07367.1"
                     /translation="DKLRNSIMDIAGVSEIFKALSDETRTRILYLISQRELCVCDLAF
                     LLDMTLPAVSHHLRFLRAMRLVKARKEGKNVFYSLDDNHVLALIEQAKEHYIERT"
     gene            379..2568
                     /locus_tag="PLS88_08215"
     CDS             379..2568
                     /locus_tag="PLS88_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOU07368.1"
                     /translation="MIEHRDNEHLCLICEEDRQKKISERQEAPDEAEEPMVSYAMLKG
                     FSSALGEPEVLDANLKASNLALGEPEAPKESRENKTSNKTLARIVLAGVLFLIGSIFN
                     TALHTTPFSIAEYIVLVPAYLLVGGPVLLAAFRNIRHGKVFDEMFLMSIATIGAMIIH
                     QLPEAVGVMLFYAVGEYLQDLAVDRSRGSIAALMDLRPESARIFEKNEGSKEADELNK
                     IDGLEYSGTGIRRLVSPEEVPVGALIEVLPGERIPLDGEVIEGSSAVDTSSLTGESIP
                     RNVNPGDKVLAGFVNDGGRLLVRVEKGFGQTAVARILELVEEAESHKAPTEKFISRFA
                     AIYTPVVVAIAAIIAIVPPIVMPSPSFAAYLSKWVYRALVLLVISCPCALVVSIPLSY
                     FGGIGSAARKKLLIKGANHIDTLAKVDTIVFDKTGTLTEGHFSVLEVVPRNGFSKEQL
                     LIWASAAESKSMHPIARSICAARSIYAGAISKEQEAEDIQELKGFGVIARIGGHEIIV
                     GNDRLLHKEKIIHEDCDVDGTVAYVIVDRSYAGYLIIADAIKQGAAEAIASLKQLGLR
                     KTAMLSGDSESVAKNVAKRLGIDEFYAELLPDQKVAQLDKLKEDPKRDGSIVFVGDGM
                     NDAPVLIHADLGIAMGGLGSDAAIEAADVVVMDDDIRRIPLALRIAKFTRKIVLQNIV
                     FALSVKGAFIILGSIGVANMWEAVIADVGVSLLAVMNSLRTIGHRYS"
     gene            complement(2656..>3001)
                     /locus_tag="PLS88_08220"
     CDS             complement(2656..>3001)
                     /locus_tag="PLS88_08220"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HOU07369.1"
                     /translation="SGSVSMISQLLVAYVLVATSNVESLKQRPEVIPVALGYVVALLA
                     FTTGLSFILNRFFHIDLLTAMYSTAPGGISGMAITAVEAGADTAISSAFQVLRIVLVL
                     VATPLLAAILSK"
BASE COUNT          772 a          665 c          778 g          786 t
ORIGIN      
        1 gataagcttc gaaattcgat catggatatc gcaggggtct cggagatatt caaagcactg
       61 tcggatgaga cacggactag gattctctat cttatttcgc agcgcgaatt gtgtgtctgt
      121 gatctcgctt ttctcttaga tatgacctta ccggcagtct cgcatcatct tcgatttctg
      181 agggcaatgc gccttgtaaa agcaagaaaa gaaggtaaaa acgtttttta tagccttgat
      241 gacaatcatg tgctagctct catcgagcag gcaaaagagc attatatcga acgcacttag
      301 agtcaacttt catgaaccat aaaccttacc cgaaaaatcg aggatctgaa aaccatctga
      361 aagccaagag aggtttctat gatcgaacac cgagataatg agcatctatg cttgatctgc
      421 gaagaagata gacagaaaaa gattagcgaa aggcaggagg cgcctgatga ggcagaggag
      481 cccatggtat cctatgcaat gctcaaaggg ttcagttcgg cgctcggaga acccgaggtg
      541 ctcgatgcaa atctcaaagc gtccaatttg gcgcttggag aacccgaggc tcccaaagag
      601 tctagggaga ataaaacttc aaataagact cttgcaagga ttgttctagc cggagtcctt
      661 tttctcatag ggtcgatttt caatactgcg ctccatacta caccattttc gatagccgaa
      721 tacattgtgc tcgtccctgc ctatctactc gtcggaggcc ctgttctctt ggctgctttt
      781 cgaaacatac gccatggcaa agtcttcgat gagatgtttt tgatgagcat tgctactata
      841 ggcgcgatga taattcatca gttgccggag gccgttggag tcatgctttt ctatgcggtc
      901 ggagaatatt tgcaagactt agctgtcgat cgttccagag gctcgattgc ggctctcatg
      961 gatcttcgcc ctgagtcagc aaggattttc gaaaagaatg agggatccaa ggaagctgat
     1021 gagctaaata aaatcgacgg gctagaatat agcggaacag gcattcgccg acttgtctct
     1081 cctgaggaag tccctgttgg agcgctgatc gaggtattgc caggggagag gattcctctc
     1141 gacggcgagg tgatagaagg atcttctgcg gtagacactt cctctcttac aggcgagtcg
     1201 ataccaagga atgtgaaccc aggagacaag gtattggccg gctttgtaaa cgatggaggc
     1261 agactccttg tacgtgtaga gaaagggttt ggccagacgg ctgttgctcg gattctcgag
     1321 ctggtggagg aggctgaatc tcataaggct cccacagaga aatttataag ccgctttgcc
     1381 gcaatctaca caccagttgt ggtagccatt gccgctatta ttgccattgt tcctcccatc
     1441 gtcatgccga gcccttcttt tgcggcttat ttgtctaaat gggtctatag agctctcgtt
     1501 ctccttgtaa tatcttgccc ctgtgccctt gttgtttcta ttccattgag ttatttcggg
     1561 gggataggaa gcgcagctcg caagaagctt ctcataaaag gagctaatca tatcgatacg
     1621 cttgcaaagg tcgatacgat cgttttcgat aagacgggca ctttgaccga agggcacttt
     1681 tccgtgctcg aggtcgtgcc tcgcaatggc ttttcgaagg aacagcttct tatatgggct
     1741 tctgctgcgg aatcaaaatc catgcatccc attgctcgtt ctatttgcgc ggcgcgctct
     1801 atctatgccg gagctatttc gaaagagcaa gaagccgaag atattcaaga gcttaaaggc
     1861 tttggcgtga ttgcaagaat aggtggtcat gaaatcatcg tgggcaatga tcggcttctc
     1921 cataaagaga agattatcca tgaggattgc gatgtcgatg gcacggttgc gtatgttatt
     1981 gtcgatcgta gctatgctgg ctaccttatt attgcagatg caatcaagca gggtgcggcc
     2041 gaagctattg cgtctttgaa gcaactaggc ttgcgcaaga cagcaatgct ctctggcgat
     2101 tccgaatcgg tggcgaaaaa tgtagcgaaa aggctcggta tcgatgagtt ctacgccgaa
     2161 ctcctgccgg atcaaaaagt tgcacagctc gataagctca aagaagaccc aaaaagagat
     2221 gggtctatag tctttgtggg cgatggaatg aatgatgcgc cagttctgat ccatgccgat
     2281 ctgggtattg caatgggtgg gctaggttcc gatgcggcca tcgaagccgc ggatgttgtt
     2341 gtgatggacg atgacatccg aaggattccg cttgccttgc gcatagctaa atttactcgc
     2401 aagattgtgc ttcagaatat cgtttttgct ctttcggtaa agggggcatt cataatactt
     2461 gggagcattg gcgttgccaa tatgtgggag gcggtgatcg ccgatgtcgg cgtgtcgctc
     2521 ttggctgtca tgaattcgct acggacgata ggacatagat attcgtgatt ttgaaatgtc
     2581 catagccaat tagcaatagc caaagactat tcgctctgga cgacgtgaag ggattctgat
     2641 gctgttctct ttgtgctact ttgacagaat tgccgccaat agcggcgtag cgacgagaac
     2701 gagcacaatt cgcagtacct gaaatgccga ggaaattgcc gtatcggcgc cagcctccac
     2761 agcggtaata gccattccgc ttatgccgcc cggtgcggtg ctatacattg cggtcagcaa
     2821 atcgatgtgg aaaaatcgat tgagaataaa agataagcct gtggtgaaag ccaatagcgc
     2881 tacaacatat ccaagagcca cgggaatcac ttctggcctt tgttttagcg actccacatt
     2941 tgatgttgcc accaaaacat atgccacaag tagctgcgaa atcattgaga cagatccact
     3001 g
//
LOCUS       DAOSOB010000106         2973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_33237_length_2973_cov_103.305346, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000106 DAOSOB010000000
VERSION     DAOSOB010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33237_length_2973_cov_103.305346"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PLS88_08225"
     CDS             complement(<1..311)
                     /locus_tag="PLS88_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU07370.1"
                     /translation="MEKYIEIIDAVKTFGTIEALRGISITVRKGEVVLIIGPSGSGKS
                     TLLRCINRLERLDSGEILIDGESVTDPDSDIRHIREKVGMVFQSFNLFPHLTVLDNIT
                     LA"
     gene            complement(295..1116)
                     /locus_tag="PLS88_08230"
     CDS             complement(295..1116)
                     /locus_tag="PLS88_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOU07371.1"
                     /translation="MKGHSSSEKNSKSSEKTTINVTDGTLIPIKDEGSLMSAWNISFF
                     GAIAMLIILPLISPDPYLRILSFVPDGLIATFSVTIISIFFALIIGLFAGLGRISQNL
                     VINRIATVYVEVIRGIPLLVQLFYIYYALGPLLRLQGHTAAVLAMSICYGAYLAEIFR
                     AGMQAIPRGQMEAALALGMSRSLAMRKIIIPQTIRIVLPPIGNEFIALLKDSSLVSIL
                     AVSDLLRRGREFASTTFRYFESYTVVALIYLVMTLFFSRLVAIMEERLRHRGKVH"
     gene            complement(1156..1917)
                     /locus_tag="PLS88_08235"
     CDS             complement(1156..1917)
                     /locus_tag="PLS88_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU07372.1"
                     /translation="MKKLRSISTTVALALLVALAAGSLFSCAKKPTKIVVATDATWPP
                     MEYIDENKNIVGFDIDLVNEIAKAGGFEVEFKNTAWDGIFAGLATGNYKVIASSVTIT
                     EERKATMDFSEPYVNAGQVLVVRKETTGVSSLADMVGKKVGAQIGTTGAIEISKVSGV
                     ELKTYDEIGLAFEDLANGNIDGVVADSPIAANFALQNASYKERLKIAGEPFTDEWLGF
                     VFKKGDTKTQKLFNDGLAKVKESGKLDELIQKWLK"
     gene            complement(2101..>2973)
                     /locus_tag="PLS88_08240"
     CDS             complement(2101..>2973)
                     /locus_tag="PLS88_08240"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOU07373.1"
                     /translation="AIEGYHSEQLHKVYGHIDEYITEPETTNDDVVYELAFLDALEKN
                     GRNLTSRDIALEWIRQIPFGWSAEWIALHNLADGIFPPESGSWHNPYSNWIGAQMRGM
                     VCGLVSPGWPIEAARLAHLDGVVSHAENGVYGEIYAAVLTSLAFVLSDPRTLLTDAAE
                     FIPAKSEYAAVLAKCLETVRSNAQPEAAWKKLDVYFERYNWIHAYPNIAAVITALWFG
                     NGDMTESFRILAHAGLDVDCNAGLVGTVIGIINGVPDKWGKPLNDTLETYLSGKEHLS
                     IRKLAAITARIAAL"
BASE COUNT          753 a          689 c          742 g          789 t
ORIGIN      
        1 gcgagggtga tgttatcgag cacggttaaa tgggggaaga ggttgaagct ctggaatacc
       61 atgcctacct tttcgcggat gtgacggata tcggaatcag gatccgttac gctctctcca
      121 tcgataagaa tttctccaga atcgaggcgt tcgagacggt tgatgcatcg aagcagcgtg
      181 cttttgcctg aaccagatgg acctatgatt aggacaactt cgccctttct tacggttatg
      241 ctgatacctc ggagcgcttc tatagtgcca aaggttttta cggcgtctat gatctcaatg
      301 tacttttcca cggtgtctca gcctttcttc cattattgca accagacgcg agaaaaagag
      361 cgtcattaca agataaatga gcgcgacgac ggtgtagctt tcgaaatacc tgaaagtagt
      421 cgaagcaaat tctctgcctc ttcttaggag gtcggatacg gcgagaatcg agactagcga
      481 agaatcctta aggagcgcta tgaattcatt tccaatggga ggcagaacga ttcgaatagt
      541 ctgaggaatg atgattttgc gcatggccaa ggagcgcgac atgccgagag caagggctgc
      601 ttccatctgg ccgcgaggga tggcctgcat gcctgctcta aaaatctctg caagataggc
      661 gccatagcag atagacatgg ccagcactgc ggcggtatgt ccctggagcc gcagaagcgg
      721 ccccagagca taataaatat agaaaagctg aacaagaagc ggaattcccc gaatgacctc
      781 gacatagaca gttgcgattc gatttatgac aaggttctgc gaaatgcgcc ccaatccagc
      841 aaagagacca atgatcaagg caaaaaatat ggaaatgata gtaaccgaaa atgtcgcgat
      901 aagtccgtcg ggcacaaagg aaagaatcct aagatatgga tctgggctta tgagcggcaa
      961 gattatcagc attgctatag caccgaagaa ggagatattc caagccgaca taaggctgcc
     1021 ctcatccttt atggggatga gggtgccgtc tgttacattg atggtcgttt tttcggagct
     1081 ttttgaattc ttttcagacg atgaatggcc tttcattttt atgctttttt gaccgttcta
     1141 ttgtggtttt catgcctatt tgagccattt ctggataagt tcgtcgagct tgccagattc
     1201 cttgactttg gcaaggccat cgttgaaaag tttttgggtc tttgtatctc ctttcttgaa
     1261 gacgaagccc agccactcat cggtgaaggg ttccccggca atctttaacc tttccttata
     1321 cgaagcgttc tggagcgcaa aattagctgc aattggtgaa tctgcaacca caccgtcgat
     1381 atttccgttt gcaagatctt cgaaggcaag ccctatttca tcgtatgtct tgagttccac
     1441 accggagact ttgctgattt cgattgcacc ggttgttccg atctgcgcgc ctaccttctt
     1501 gcctaccata tccgcgaggc tgctcactcc cgtagtttct tttcggacaa caagaacctg
     1561 gccggcattt acataaggct cggagaaatc cattgtcgcc ttgcgctcct cggtgatcgt
     1621 gacagaggat gctatgacct tgtagttgcc cgtggcgagg ccagcaaaaa tgccatccca
     1681 ggctgtattt ttgaattcca cttcgaagcc gccggccttg gcgatttcgt tcacaagatc
     1741 gatatcgaat cccacgatat tcttattctc atctatatat tccatgggtg gccaggtagc
     1801 atcggtggca acaactatct tggttggctt ttttgcgcag gaaaaaaggc tacctgcagc
     1861 gagtgcgaca agcaaggcaa gcgcaacagt ggtggaaatt gatctaagtt ttttcatacc
     1921 tctgctcctt ttggacatct attggaatcc atgtcctgta ttaaagcaat ttattgtatg
     1981 aggagaatat ggcgaattgc atatagtgtc aataatctcg aataaatatg ggacttttgg
     2041 ggcatagggc gaggaagaac gcagcgcatc tgatattgga tacaaaatag cgcaatggct
     2101 tcatagagcc gcaatgcgag ccgtgatagc ggctagcttt cgaatgctga gatgctcttt
     2161 cccggataga tatgtttcaa gggtatcatt caagggtttt ccccatttat ccggcacacc
     2221 gtttattatg ccgatcacag tccccacaag gccagcgttg cagtcgacat cgaggcctgc
     2281 atgagcaagt atccggaagg attcggtcat gtcgccattg ccaaaccata gcgcagttat
     2341 gacggctgca atgttggggt aggcatggat ccagttatag cgttcgaaat acacatcgag
     2401 ctttttccag gctgcttctg gctgcgcatt cgagcggaca gtctcgagac attttgcaag
     2461 cacggcagca tattcgctct ttgcaggtat aaattcagcg gcatcggtaa ggagggtacg
     2521 ggggtcagac aaaacaaaag ctagagaagt cagaaccgcg gcatagattt ctccatagac
     2581 tccattttct gcatgcgata ctacgccatc gagatgggcg aggcgagctg cttctattgg
     2641 ccagccaggg ctcacaaggc cacagaccat accgcgcatc tgcgctccta tccaattaga
     2701 gtaagggttg tgccaagagc ctgattctgg agggaatata ccatcagcca agttgtgcaa
     2761 agctatccat tcggcagacc atccaaaggg aatctggcgt atccactcga gtgcaatgtc
     2821 gcgcgatgtc agatttcttc catttttttc cagcgcatcg agaaaggcga gctcatatac
     2881 aacgtcgtcg tttgtggttt ctggttctgt tatgtactcg tctatgtggc catagacctt
     2941 atgtaattgc tcgctatgat aaccttcgat tgc
//
LOCUS       DAOSOB010000107         2818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_35645_length_2818_cov_57.947521, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000107 DAOSOB010000000
VERSION     DAOSOB010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35645_length_2818_cov_57.947521"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLS88_08245"
     CDS             <1..541
                     /locus_tag="PLS88_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOU07374.1"
                     /translation="FHPTQALADILTIEENFGNCAGKKLCFVGDGRNNVARSLMVISA
                     KLGMHFTIVSPRELWPDSELENLCESFAKASGSTLTITDEVDNGVKGADAIYTDVWVS
                     MGEEALKAERVKLLAPYQVNAELMAKTGNNACIFLHCLPAVKGEEVTFDVIEGPQSKV
                     FDEAENRKHTIKAIILATL"
     gene            573..1559
                     /locus_tag="PLS88_08250"
     CDS             573..1559
                     /locus_tag="PLS88_08250"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOU07375.1"
                     /translation="MKQVPRALIDFINHHDCFYILGHREPDGDCIGSQLALASMLQGM
                     GKRVHILSSGPFNRIEILPFEARFKSEVPGERDFERTAAIVLDCSSMERIGNIAERMP
                     NIPASFIDHHSTSSVPGPYDWLDESASAVSAMVLLLMDAMGHSPTKEEAELLFFGLCT
                     DTGFFRHLDEHGSESFRIAARLVEAGVSPKRTYMAINGGRTLASRRMLGELLLRIQPY
                     YEGRLLVSWVTIEDQQKYGMSSRDSDLLYQLMMSIVDCEVCFVVKQETNDMCTVGLRS
                     KNSINVARIAEQYGGGGHRLASGLSINGNVDEVVHTLVGAFADVFSNEETGK"
     gene            1989..>2818
                     /locus_tag="PLS88_08255"
     CDS             1989..>2818
                     /locus_tag="PLS88_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07376.1"
                     /translation="MDSQTYLDLVRKMYEHPKRYGFFTKDEISDALNHYRNRIELIID
                     RAENESRTKDAYLLSSMRYVAKSVHRQNFNLSLCETAYIYSNFTEDLTIESPIEPWTD
                     YETTQDIATEKGILNLAPQVILDKLTPARKRLLFLILKCAWEIDEELLNRCSLSLGIP
                     KQYLFNLIELTKRRTEGCWNKAEQMNIKLNTLWIRLRVLEFRLETCLLNEEKEYILCS
                     LKRCRARYTRLLEKRSHRRSAVSNESISEILGVPKGSVDSGLFYLRKHVDSGRSLEDY
                     "
BASE COUNT          835 a          537 c          620 g          826 t
ORIGIN      
        1 atttcatcca acgcaagctc ttgccgatat cctcactatc gaagaaaatt ttggcaactg
       61 cgcaggaaag aaactctgct ttgtcggtga tggacgcaat aatgtagctc gctcgctaat
      121 ggtaatcagc gcaaaactgg ggatgcattt caccatcgta tctccccggg aactctggcc
      181 cgatagtgag ttagagaatc tatgcgagag ctttgctaaa gcatcgggtt ctaccttaac
      241 aatcacagat gaggtagata atggagtcaa aggagctgat gctatatata ctgatgtttg
      301 ggtttcgatg ggggaagagg ctcttaaggc tgaacgagtc aaattgctag ctccatatca
      361 ggtcaacgcc gagcttatgg caaagaccgg aaataatgcc tgtatttttt tgcattgtct
      421 gccggcagtg aaaggggagg aagtgacatt cgatgtcatt gaaggacctc agtcaaaggt
      481 attcgatgag gctgaaaata gaaaacacac tataaaagcc ataatccttg ctacactgta
      541 gcatcttgga ataggaatct ataatattta acatgaaaca agttccacga gcacttatag
      601 actttataaa tcatcacgat tgtttttata ttttaggtca tcgtgaacct gatggcgatt
      661 gtatcggttc tcaacttgca cttgcttcta tgcttcaggg tatgggaaag cgtgtccata
      721 ttctctcttc tggaccattt aataggatcg agatacttcc atttgaggcg agattcaaaa
      781 gcgaagtccc aggcgaaaga gattttgaga gaacagcggc cattgtgcta gattgctctt
      841 cgatggagag aatagggaat atcgccgaga ggatgcccaa tattcctgcc tcattcattg
      901 atcatcattc gacctctagt gttccaggcc catatgattg gttggacgaa tcggcttcgg
      961 cagtgtcagc aatggtcttg cttcttatgg atgcaatggg tcatagcccc accaaagagg
     1021 aagcagagct tttatttttt ggtttatgca cagatacagg ctttttccga catttggatg
     1081 aacatggaag tgaaagcttt agaatagcgg ctcggctcgt cgaagccggc gtttcgccta
     1141 agcgtacata tatggctatc aatggaggaa gaactctggc ttctcgccgc atgctaggcg
     1201 aacttctcct tcgtattcag ccatactacg aaggcaggct tctcgtttct tgggttacca
     1261 tagaagatca acaaaaatat ggtatgtcta gcagagactc ggatttgcta taccaactaa
     1321 tgatgagcat agtcgattgc gaagtctgtt ttgtagtgaa acaggagacc aacgacatgt
     1381 gtacagttgg tcttaggtca aagaattcta taaatgtcgc tagaattgcc gaacagtatg
     1441 gaggtggtgg gcatcggctt gcttcaggcc tttctatcaa tggtaatgtc gatgaagtcg
     1501 tccatacact agttggagcc tttgcagatg tcttcagcaa tgaagaaacc gggaaatagc
     1561 gatccaatag cgagaaggtt atctatagct gattttacca caataagcat aattatgtgc
     1621 ttattgtgca ttatccttga ctagagaaag tcaattcttt ctattaagaa gggcaataat
     1681 aatttccata aaaccgcact ttgtatgaaa aggtaaactc tttgctttag aaagcagtat
     1741 tctcaaatat tcttttttat cttgttcgtc aatggtatta ccaagttttt accttctata
     1801 aatgaggacc acactgtaat aatttaaaat tgtctttcaa tccagtattt cagctcaatt
     1861 cattttttat taagtttgga aatgtcaaat aattcttgat ttaatcaaaa ataaaatatt
     1921 gtttctgtat ttatggtaat ttttctgatt tggcacatcg tttgcagact agaatcggag
     1981 gctcaagaat ggattctcag acttacctcg atcttgtccg caagatgtat gaacatccaa
     2041 agcgctatgg cttttttaca aaggacgaaa tatcggacgc gctcaatcat tatcgaaacc
     2101 gcatcgaatt gataatcgac cgggctgaaa acgaaagcag gacaaaagat gcatatctat
     2161 tgagcagtat gcgatatgtt gcaaagagcg tacatcgtca aaattttaac ctcagtctct
     2221 gcgagactgc ttatatatat tccaatttca ctgaagacct cacaattgaa tctccaatag
     2281 aaccctggac tgattatgaa acaacgcaag atatagcaac tgagaaagga attttaaatc
     2341 ttgctcctca ggtaatttta gataaattga cccctgctcg caaaaggctg ctttttctaa
     2401 tattgaaatg cgcttgggag atcgatgaag aacttttgaa taggtgtagc ctttctttgg
     2461 gcatccccaa gcaatacctg tttaatctaa tcgaattgac aaagagaaga accgaaggat
     2521 gctggaataa ggcggagcaa atgaatatca aattgaatac gctatggatt cgactccgtg
     2581 tacttgaatt cagacttgag acttgtcttc tcaatgagga gaaagagtat attctatgct
     2641 ctcttaaacg atgtcgggcg cgctatactc ggcttttaga aaagcgcagt caccgcagat
     2701 cggctgtttc gaatgaatca atttcggaaa tacttggggt accgaagggt agtgtagatt
     2761 caggcctttt ttatctcaga aaacatgtcg attctggtag gagtcttgaa gattatag
//
LOCUS       DAOSOB010000108         2723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_37294_length_2723_cov_75.779985, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000108 DAOSOB010000000
VERSION     DAOSOB010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37294_length_2723_cov_75.779985"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLS88_08260"
     CDS             <1..885
                     /locus_tag="PLS88_08260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU07377.1"
                     /translation="LERFGKAIRNPAKTTIVSFAAAEVGAGKGFALQEVLDQIGAFAG
                     PFMLFLVLTLKGNNNKISAYQLSFALLAIPAAIALILLLLARKNYPDPQEFEAPKEAL
                     PQKGLNSAYWYYLVGIGFLALGFADFPLMAFHMTKTKLLPDNVVPVLYSLAMGIDALS
                     ALYFGRMYDRRGITAIIISSAITLFFAPFVFLVGTPVGAIAGVMLWGIGMGAQESILK
                     SAVTTIVPKERRGTAFGIFNAGFGAFWFVGSWIMGLLYDRSLLALVLFSMLGQLIALP
                     FFFKTRSLLKHSLHIRQS"
     gene            complement(854..>2723)
                     /locus_tag="PLS88_08265"
     CDS             complement(854..>2723)
                     /locus_tag="PLS88_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOU07378.1"
                     /translation="LSFSLNEKAGYLSEYLIRQLKRTNVRSDYATDSIFANFRSIATT
                     GIKPGVIYRSSNPINNEIGRAAYADALCRAVGIQTVINLSDSNEDIAKYLSAPGFKSD
                     YYKSLLDSGKVVALNMGVDILGADFAAKFAQGVRFIIANDEPYLIHCTEGKDRAGFVS
                     AVLEALMGADLQEIVADYMKSYENYYGVKTGSDQYDAIANSNIVASLTTAMCGLAKGV
                     DISGINLSKFAEWYLLSIGLKQGEINALKAKLSAEPMVKEPWIKGKVTQIEKYGHALA
                     DITIQDFNNLGFAFGDLVTVIFDNGFVLEAPYLDGYYVNAGDPLVRAYPGHTNIGICI
                     NYGKLNEVAQVKVGSGFIIMLSNPGAYRTQYEIRKLVRSNKREDYASDEIFANFRNIA
                     VGNIAEGVLYRSSSPIDNQLGRAAYADKLIEEAGIETVVNLSDSIDAMKTYLAAKDFA
                     SPYYAELAKNNKVIFLNMNLAYGSEEFRANVAKGLVFMVEHDGPYLVHCTEGKDRTGF
                     ISALLEALMGATKDEIVVDYMESYINYYGVEPGTEKYNLIAQDVLDMLKVITGTGDLE
                     KADLQAGARNYLIQGGMKPEQIEKLMNKLSKSISSSENSEFPAFFLAMIDEYVASA"
BASE COUNT          667 a          650 c          620 g          786 t
ORIGIN      
        1 ctcgagcgct ttggaaaggc aataagaaat cccgcaaaga cgacgatcgt ttccttcgcc
       61 gccgcagaag tcggtgctgg caaaggcttt gctttgcagg aagtgctcga tcagattggc
      121 gcttttgcag gaccattcat gctctttctt gttctcacac taaaagggaa caataataag
      181 atttcggcgt accaactcag ttttgctctt cttgccattc ctgcggctat tgcattgatt
      241 ttgcttctgc tcgcaagaaa aaattatcca gatccccagg aattcgaggc accaaaagaa
      301 gcattacccc aaaaaggcct aaattcggca tattggtact atcttgtagg aattggattt
      361 ctcgcccttg gctttgccga ttttccactc atggcctttc atatgacaaa gacaaaacta
      421 ttgccggata atgtagttcc ggttctgtac tcgttagcta tgggcatcga tgcgctctcc
      481 gcgctgtact ttgggagaat gtacgatcga agaggaatta cggcaatcat catttcttcg
      541 gctatcacct tgttttttgc gccgtttgta tttcttgtag ggacccctgt tggcgccatt
      601 gctggcgtaa tgctatgggg tataggcatg ggcgcccagg aatctattct aaaatctgcc
      661 gtaacaacaa ttgtgcccaa agaacgcaga ggcacggcct ttggcatttt caatgcaggg
      721 ttcggggctt tctggttcgt gggaagctgg atcatgggcc tactatacga tagatctttg
      781 cttgctcttg tccttttctc tatgcttggc cagctgatcg cgcttccctt cttcttcaag
      841 acaagaagcc ttcttaagca ctcgctacat attcgtcaat catagccaag aaaaatgctg
      901 ggaattcaga attctccgag gagcttatgg atttcgagag cttattcatc agcttctcaa
      961 tctgttcggg cttcataccg ccctggatca gataatttcg agctccggct tgtaagtcag
     1021 ctttttcgag atcgccggtt cctgtaatca cttttagcat atcgagtaca tcttgcgcga
     1081 tcaaattgta cttctcggtg ccgggttcta ctccatagta gttgatataa ctctccatat
     1141 aatccacgac gatctcatcc ttggttgcac ccataagtgc ttccaatagc gcggagataa
     1201 agcctgtgcg atccttgcct tcggtgcaat gtacaaggta tggaccatca tgttcgacca
     1261 tgaaaacaag gccttttgct acgttcgccc tgaattcttc acttccatac gctagattca
     1321 tattgaggaa aataacctta ttattctttg cgagttccgc atagtaaggc gatgcaaaat
     1381 ccttggctgc aagataagtc ttcattgcgt ctatggaatc cgacaagttg actacggttt
     1441 cgatgcctgc ttcctcgatc agcttatcgg cataggctgc ccttccgagc tgattatcga
     1501 tggggcttga actgcgataa agcacaccct ccgcaatatt tccgacggca atattgcgaa
     1561 aattggcgaa gatttcatcg gaagcataat cttctctctt gttggagcgc accagtttgc
     1621 ggatttcata ttgcgttcga taggctcctg gattgctcag catgatgata aagccactgc
     1681 ctacttttac ttgagccact tcgttcagtt ttccatagtt gatgcatata ccgatatttg
     1741 tatggccagg ataggcacgc acgagcggat cgccggcatt tacataatag ccatcgagat
     1801 acggcgcctc gagcacaaaa ccattgtcga agataaccgt aacaagatcg ccaaacgcaa
     1861 aaccaaggtt attgaaatcc tgtatggtga tatcggcgag ggcgtgccca tatttctcga
     1921 tctgtgtgac tttgccttta atccaaggct ctttgaccat tggctcggcg gagagctttg
     1981 ctttgagtgc atttatctcg ccttgtttga gcccaatgct caaaagatac cactccgcga
     2041 atttggaaag attgataccg gatatatcga cgcccttcgc aagtccgcac atcgcggtgg
     2101 tcaatgaggc aacaatattg ctattagcaa tggcatcata ttgatcgctt cctgtcttta
     2161 cgccgtagta attctcatag ctcttcatat agtcggcgac gatttcctgt agatccgcac
     2221 ccatcaatgc ttctaggacg gcactgacaa agccggctcg atctttacct tcggtgcaat
     2281 gaatgagata aggctcatcg tttgctataa taaagcgaac accttgggcg aattttgcgg
     2341 caaaatccgc acccaggata tccactccca tgttgagcgc aacgacctta cccgaatcca
     2401 ggagagattt gtagtaatca gatttgaatc cgggagcaga gaggtattta gctatgtctt
     2461 cattggaatc ggatagattt attacggttt gtatgcctac ggccctgcag agcgcatcgg
     2521 cataggcggc tcgtcctatc tcattgttga ttggattgct gcttcgatag ataacaccag
     2581 gctttatgcc tgtagttgca atgctcctga agttggcgaa aatcgaatcg gtagcgtagt
     2641 cggatcgaac attagtgcgc ttgagttggc gaatcaagta ttcagagaga tagccagcct
     2701 tttcatttag cgagaaagaa agc
//
LOCUS       DAOSOB010000109         2713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_37483_length_2713_cov_121.870203, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000109 DAOSOB010000000
VERSION     DAOSOB010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37483_length_2713_cov_121.870203"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2057
                     /locus_tag="PLS88_08270"
     CDS             <1..2057
                     /locus_tag="PLS88_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU07379.1"
                     /translation="PQATVSLSRSIFSPNGDGRADTVELTQSSVYGDRWSGQIISETG
                     KIVRTWEWYPMLADVIWDGKDQSGKIVSDGAYYYELRSIDEAGNSFILPRQQIIVDAT
                     QKNASFKVEPIAFSPNDDGVKDLIYLNVNAPKPDTLLSYSLSIFAGSKTTGGQAPVRS
                     WKGKTDIKSQYVWDGMTDAGLKVPDGYYSAYLALEYANGDIFNFEPIQILVDTVPPKI
                     SISADPLLFSPNGDGIKDTVTITQESEPGDDWSGRIRSASGATVRSYSWKGQAKSFTW
                     DGTDLNGKLVPNGAYSYEVVSVDVAGNSASASIKGITVDATKPRVFVTASDNGISPND
                     DGIRDEVSFSLTVENREGVESWRFSLIDSKGVERSFFGGSDSDVPTRLVWDGRDLHGQ
                     VIEDTFTGKLVVKYLKGDIADATSNKIVVKIAPPQVEIKVRPDYFSPDDDGVDDILTF
                     QIVADKNAGITEWKLEVLETAIVESATLQESKPTRSFMLWSGKGMPPLQITWNGKSPK
                     GELVESATDYQFEFSCWDALGNQTKIKGIIAVDVLVIRDGDRLKIRVPSIVFRANHAD
                     FIGLDSEIVARNEKVVARIAQILGKFPDYRIRVEGHGNNVGKMLGYSASRIQQEEVNE
                     LIPLSTERAEVVRKMLVENGVDPRRLTVVGLGSSEPVVPFTDVQNRWKNRRVEFVLIK
                     NQ"
     gene            complement(2181..>2713)
                     /locus_tag="PLS88_08275"
     CDS             complement(2181..>2713)
                     /locus_tag="PLS88_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07380.1"
                     /translation="SELLDQLYRERCSAYSSPLILITDEKCEYARALKLHPSRFSIHH
                     LTVNSKVPRTFQNPLFASNYLDRELRKDLADHRRETVCFPRNVANMLNRLVVYLGWHN
                     YEKPYRIGRDIVLTHAEVAGIEREAICKAREKQFQERAFLSRAGLSLLDKKLWLRSFP
                     TPLKRKAEYVPAYAYA"
BASE COUNT          772 a          535 c          671 g          735 t
ORIGIN      
        1 cgccacaagc gactgttagt ctttctcgct cgatattttc tccaaatggg gatggacgag
       61 ccgatactgt tgaattgacg cagagcagcg tctatggaga tcgctggagt gggcaaataa
      121 tctcagagac aggaaagatc gtccgcacct gggagtggta tccaatgctc gccgatgtta
      181 tttgggatgg caaagatcag agtgggaaga ttgtatcaga tggtgcttat tattatgaac
      241 tgagatcaat agatgaagct ggtaattctt tcattcttcc tcgccagcag ataatagtgg
      301 atgcaacaca gaaaaatgca agtttcaaag ttgaacccat tgcgtttagc ccaaatgatg
      361 atggtgtcaa agatcttatt tacttaaatg tcaatgcacc aaaaccagac acactcctct
      421 cctattcact ttctatattt gcaggttcaa agaccacagg aggtcaggcg cctgtacgaa
      481 gctggaaagg gaaaaccgat atcaagtcgc aatatgtttg ggatggtatg acagatgcgg
      541 gattgaaagt gccagatggg tattatagtg cgtatcttgc tctagaatat gcaaatggag
      601 atatcttcaa ttttgagcct attcagatac ttgtcgatac agttcctcca aagataagca
      661 tatctgcaga tcctttgctc ttctccccga atggtgatgg cattaaggat actgtgacca
      721 ttactcaaga atcagagcca ggcgacgatt ggtcaggtag aattcggagc gcatctgggg
      781 caacagtacg aagctattcc tggaaaggac aagctaagag ctttacttgg gatggcacag
      841 acttgaatgg gaaacttgta ccaaatggtg cttattccta tgaggtagtc agcgtggatg
      901 ttgctggtaa cagcgcttct gcttctatca agggaattac agtcgatgcc acaaaaccga
      961 gagtttttgt aactgcttca gacaatggaa tttcgcccaa tgatgatgga attcgagatg
     1021 aagtatcttt ctctcttact gtagagaacc gcgaaggtgt cgagtcatgg cgattctctc
     1081 ttattgattc taagggtgtt gaacgctcct tctttggcgg gagtgactca gatgtgccta
     1141 cacggcttgt gtgggatggc cgagacttgc atggccaagt gatcgaagat acttttacgg
     1201 gcaagcttgt ggttaagtat ctgaaaggcg acatagcaga tgcaacaagc aacaaaatag
     1261 tggtcaagat tgcgcctccg caggtagaga tcaaggtcag gccagattac ttcagccctg
     1321 atgatgatgg tgtcgatgat atactcacct tccaaatcgt ggctgataag aatgcaggca
     1381 taacagaatg gaaactcgaa gtgctagaaa ccgctattgt cgaatctgcc accttgcagg
     1441 aaagcaaacc gacgcgatct ttcatgcttt ggagcggcaa aggaatgcct cctttacaga
     1501 tcacctggaa tggaaaatcc cccaagggag aattagtcga gtcggcaacc gattatcaat
     1561 ttgagttctc ttgctgggat gctcttggaa atcagactaa aataaaggga ataattgcgg
     1621 tagatgttct tgttatacgc gacggtgata gactcaagat cagagtacct tctatagtct
     1681 ttagggcgaa ccatgcggac ttcataggcc tggatagtga gattgtggct cgaaacgaaa
     1741 aagtagtcgc tcgaattgca cagatcctag gcaaatttcc agattatagg ataagggtcg
     1801 aaggccatgg aaacaatgtt ggcaaaatgc ttggatacag tgcatcgcgc attcagcaag
     1861 aagaggttaa tgagctaatt ccattatcca ctgagcgagc agaggtagtt cgtaagatgc
     1921 tagtagaaaa tggggtagat ccgcgtcgct tgactgtggt aggtcttggt tcgagcgaac
     1981 ccgttgttcc cttcacagat gttcaaaacc gctggaagaa taggcgtgta gagttcgtgc
     2041 taatcaaaaa tcagtgattt tatataacaa acggatggat ttaaagggct gcccattaag
     2101 ttgggcagcc ttttttttag cttagaatga ttactgttac gaaccttgtg tattttctaa
     2161 gagatttggg aggaagttta tcatgcataa gcataagcag gtacatactc cgcttttctc
     2221 ttaaggggag ttgggaaact tctaagccat agctttttat caagaagcga taatcctgct
     2281 ctgcttagga aagcgcgttc ttggaactgc ttctctctag ccttacaaat cgcttctctt
     2341 tcaataccag ctacttccgc atgcgtcaga acgatatccc ttccaatcct gtaaggtttt
     2401 tcataattat gccaacctaa gtatacgaca aggcgattca gcatattcgc aacatttctc
     2461 ggaaaacaaa ctgtttcgcg acgatgatca gcaagatcct ttctaagctc tctgtcgaga
     2521 tagtttgagg caaagagagg attctggaag gtacgtggga ctttggaatt tacggtgaga
     2581 tgatgtatag agaagcgact agggtgtagt ttgagcgctc tagcatattc acacttctca
     2641 tctgtgatga ggattagcgg cgaagagtaa gcactacagc gttccctata taactgatct
     2701 agaagctctg aga
//
LOCUS       DAOSOB010000110         2681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_38072_length_2681_cov_62.302742, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000110 DAOSOB010000000
VERSION     DAOSOB010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38072_length_2681_cov_62.302742"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..1494)
                     /locus_tag="PLS88_08280"
     CDS             complement(241..1494)
                     /locus_tag="PLS88_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU07381.1"
                     /translation="MKKIAGLAITLLLLVGTTAVTAQSGQKPLEITFWSLFTGGDGEF
                     FDAMVKEFNASQNEIVMKTDTVKFDDYYTKLTAALAAKTAPDVVVMHQDRLSTFVPSG
                     VLLPLDTYVQKAGIDLSTFEEAPLNNCKINNKLYAITLDVHPIIMYCNMDLFSKAGIT
                     KLPESLSELISTAETIQNKTGAIGIAADNTTATYKAYTLTRMFLSMLMEQGVYVFNAD
                     NTKANFNNEAGIKSYQALSDIVNKYKLTPKGYDYDSSVADFRLGKAAIHFNGVWVTGL
                     FEQQPNLNFVAVKFPALFGKNAAWAGSHTFAIPAQKSVDEKKIIGAMKFIDWMTNHGY
                     LWAKAGHVPTRKTAVNTKEFKSLPHRADYADAAKYVFATPATPKWPQCYDVMSDQLEE
                     SIALNRDAKTALTKLEQAINDILKK"
     gene            complement(1518..2525)
                     /locus_tag="PLS88_08285"
     CDS             complement(1518..2525)
                     /locus_tag="PLS88_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU07382.1"
                     /translation="MAASLKQLAAASGYSINTVSRALRDKSDVNPKTKEKIRNLASEL
                     GYVNNNVAKSLRTGKTNTIGVISADSSNPFFAEVILGIENTARQFGYHILLTNTEESP
                     EREREAIKILLGKQVDGLLLMPVLQQYGSCDYLKSLSIPYIIVGRWLPGLEDHSVISD
                     EYEKAKEATLYFIKNGHRDIVHLAGPSCVSSSVDRMAAFRDAHIETNTPLKEDMIIET
                     KGHLEDGYHEVSSLIRSEKHISAIFAFNDLLALGALRALKENGIKVPKEVEVIGFDDL
                     NFSKYLYLSLSSVSIPKKTMGNMAFRALLDHIENPKAPYTKKVLESRIVYRETTLVHE
                     P"
BASE COUNT          736 a          536 c          546 g          863 t
ORIGIN      
        1 acttttgagg atagaatgtt ccagtcaaag aaacttagat agactccgta tattaccgga
       61 aatacgagga aagcgacgta aatggccaaa taaggcagcg agaataagac aatatctctt
      121 ttcttgtgat cggcataatg tgatgacatt caaagctccg gaatatattt gaaaagcaac
      181 gcttgaagag gcaaaagccc cctcaagcgt catatagaaa tatcgactgt tttacaagag
      241 ctattttttc aaaatatcgt tgatcgcttg ctctagcttt gtcaaagctg tctttgcgtc
      301 tctattgaga gcaatggact cctcaagttg atcagacatt acatcatagc attgaggcca
      361 cttgggagtt gctggggttg cgaatacata tttagctgca tctgcataat cggctctgtg
      421 tggcaaggac ttgaattctt ttgtgttgac agcggttttt cttgtaggta catggccagc
      481 ttttgcccat agatatccat gattggtcat ccagtctatg aatttcattg cgccgatgat
      541 ctttttctca tcaaccgact tctgcgctgg tatagcaaat gtatgggatc ctgcccaggc
      601 ggcattctta ccaaataatg ccgggaattt tactgccaca aaattgaggt taggctgttg
      661 ctcgaaaaga ccagtgaccc acacgccatt gaaatggata gctgcttttc caagtctgaa
      721 atcagctaca gaagaatcat aatcataacc ttttggggtc aatttgtatt tatttacaat
      781 gtccgataac gcttgatatg atttgatacc agcctcgttg ttgaaattgg ctttcgtgtt
      841 gtcggcatta aaaacataca caccctgctc catcagcata gaaaggaaca tcctggtaag
      901 agtgtaggcc ttataggttg ccgtcgtatt gtctgcagct atacctattg ctcctgtctt
      961 gttttggatt gtttcggccg tgctgattag ctcggatagc gattcgggca gttttgtaat
     1021 tccggcttta gaaaacaagt ccatattgca gtacataata ataggatgga catctagcgt
     1081 gatagcgtat aatttgttgt ttattttgca gttatttaaa ggagcttctt cgaatgtaga
     1141 tagatcgata cccgcttttt gaacataagt atcgagagga agcaatacac cgctaggaac
     1201 gaatgtgctc aggcgatctt ggtgcatgac gacgacatcc ggtgcggttt tggcagcaag
     1261 ggccgcggtt agttttgtat agtaatcatc gaacttgact gtatcggtct tcattactat
     1321 ctcattctga gatgcattga attccttgac cattgcatcg aagaattcac catctcctcc
     1381 tgtaaaaaga gaccagaatg tgatttccaa cggcttttgt ccactttgag cggtgacggc
     1441 tgtagtgcct actagcaaga gcaatgtaat tgccagacct gctatttttt tcataaaaac
     1501 ctcctcaaat ttgaacctta aggttcatgc actagcgttg tttcccgata tacaattcgg
     1561 gattccaata ctttttttgt atatggagcc ttgggatttt caatgtgatc gagcaaggcc
     1621 ctgaaagcca tatttcccat tgtctttttg ggtatgctca cagaactcag gctgagatac
     1681 aaatatttcg aaaaattgag atcgtcaaag cctattactt caacttcttt ggggactttt
     1741 atgccatttt ctttaagtgc tcttaaggca ccgagtgcaa gcagatcgtt gaatgcaaag
     1801 attgctgata tatgtttttc acttcttatc aatgaactga cttcgtggta tccatcttcc
     1861 aaatgccctt ttgtttcaat tatcatatct tcttttagag gcgtattggt ttcgatatgg
     1921 gcatccctaa aggcagccat tctgtccaca gaactgctta cacaagaagg gccagcaagg
     1981 tgaacaatat ctctatggcc attttttata aaataaagcg ttgcttcttt tgctttttca
     2041 tattcatcgg atatgaccga atgatcttcc aagccaggaa gccatctgcc aactataata
     2101 tagggtatgc ttagagactt tagataatcg cagctgccgt attgctgcaa aaccggcatt
     2161 aaaagaagac catcgacctg ttttcctaaa aggattttga ttgcttctcg ttcgcgctca
     2221 ggcgattctt cggtattagt caatagtata tggtatccaa attgccttgc ggtattctct
     2281 attcccagaa ttacttcagc aaagaaggga ttcgaagaat ccgcggagat tactccgatt
     2341 gtatttgttt ttcctgtcct caaactctta gcaacattgt tgttgacata tccaagttct
     2401 gatgcgagat ttcgaatctt ttccttagtc ttagggttta catcgctctt gtcgcgcaat
     2461 gctcgagaga cagtattgat tgaatatcca cttgcagcag cgagctgctt caaagaagct
     2521 gccataatat taacgttaat gtaaacccac aatgcaaatc tgtcaatatt aaatatttag
     2581 atattatttt tccgttattt tagaaaatca atagaatatt gtggcattat gagtcatttt
     2641 ccctagataa attcatgata gattatgata acgttaatat t
//
LOCUS       DAOSOB010000111         2607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_39432_length_2607_cov_80.614028, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000111 DAOSOB010000000
VERSION     DAOSOB010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39432_length_2607_cov_80.614028"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="PLS88_08290"
     CDS             complement(<1..1111)
                     /locus_tag="PLS88_08290"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HOU07383.1"
                     /translation="MARSSAPRSLSVWLNGEKVGRWSISTAGKHEFCYDDAYISRTDA
                     CPISLSLPLVSSRFIYSGEKVEVFFDNFLPENSEIRQRIRNRFGAKSTSAFDLLAEIG
                     RDCVGALQLLQEDSQPEEIRKITGQIVSDNEVAEILRGVTGAGIRAVFPDEVFRVSIA
                     GAQEKTALLFHDGAWLIPIGSTPTTHILKLPLGRIGHMGIDMSRSVENEWLCSQIAQK
                     LDIKTAHSKISTFDGIKVLVVERFDRVISSEGNWIIRLPQEDFCQITATPSERKYEVD
                     GGPGIRSIMDILLGAVDPLGDRRTFIKSQILFWLLAALDGHAKNFSIFLEGKGHFRLT
                     PIYDIMSAYPVMGHRKDAIPRDKLKLAMAFEGKNRH"
     gene            complement(1114..1407)
                     /locus_tag="PLS88_08295"
     CDS             complement(1114..1407)
                     /locus_tag="PLS88_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU07384.1"
                     /translation="MDYLISILPQLGMSLQITRKMLGLTQREAGERVGLLPKTISALE
                     NHPASCSIESLFKYLSALELDIVLRPRKEAYNHMLEEASIAISDSQKPENVGE"
     gene            complement(1620..2384)
                     /locus_tag="PLS88_08300"
     CDS             complement(1620..2384)
                     /locus_tag="PLS88_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07385.1"
                     /translation="MDIELAIDADSRLEKVRLAIVELKGLDWQTASFKTAQNPDYVPT
                     RAKEILREIRVWPSAHFEARKKAVRDMLRNGNYKPAGRAKPSSEYILQAAIDGDFPMV
                     NFFVDAVNLASLRYLYPMSIFDSDKAGMSLLVRLGAPNEEYIFNTSGQIIDLEDLLCI
                     CALDPHIELFKEKAIEKDIISGTLNDRVICGVRGVPIANPVRDSMATKLFEGAHNAAV
                     VIYAPRNDERDLEAAAHDVAELCGQACNVPCVRILP"
     gene            2500..2573
                     /locus_tag="PLS88_08305"
     tRNA            2500..2573
                     /locus_tag="PLS88_08305"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2534..2536,aa:Ile,seq:cat)
BASE COUNT          694 a          624 c          564 g          725 t
ORIGIN      
        1 aatgtcgatt cttgccttca aacgccatag cgagcttaag cttatctcta ggaatcgcat
       61 cttttctgtg tcccatgact ggataagcag acatgatgtc atagattggc gttagtcgaa
      121 aatgaccttt tccctctaag aatatgctga agtttttcgc atgcccatcg agtgccgcaa
      181 gcaaccaaaa aagtatttgc gatttgataa aagtcctgcg atctccgaga gggtcgactg
      241 ccccaagcaa tatatccata atgcttcgta tgcctggacc gccatcgact tcgtactttc
      301 gctcggaagg cgttgctgtt atttgacaga aatcctcttg tggaagacgt ataatccaat
      361 tgccttccga agaaattact ctatcaaatc gctcaacaac aagcaccttg attccatcaa
      421 aggtcgaaat ttttgaatgc gcagttttaa tatcaagctt ttgagcgatt tgggaacaaa
      481 gccattcatt ttccacggat ctactcatat cgattcccat gtgaccaatt cttcctaagg
      541 gtagcttgag gatatgagtc gttggagtag aacctatcgg aatcaaccat gcgccatcat
      601 gaaagagtaa agctgttttt tcttgcgctc cagcaataga gactcgaaag acctcatcag
      661 gaaatacagc acgaatccca gcacctgtta cgcctctcaa aatctcagcg acctcattat
      721 cgcttacaat ctggccagtg attttgcgaa tttcttccgg ctgactgtct tcttgcagaa
      781 gctgcagagc gcccacacag tccctcccga tttcagcaag gagatcgaat gcagaggtag
      841 atttagcacc aaacctattt ctaattctct gccgaatttc tgaattttct ggcaaaaaat
      901 tatcaaagaa gacctctact ttttcacctg aataaataaa tctagaggag acaagcggca
      961 aggaaagcga aataggacat gcatcggtgc gagaaatata tgcatcatca tagcagaatt
     1021 catgtttgcc cgcagtgcta atgctccatc tgcccacttt ttcaccattg agccatacac
     1081 taaggcttct aggtgcagat gaacgtgcca tgcctattct cccacattct cgggtttttg
     1141 cgagtcgctg atagcaatgc tagcctcttc caacatatgg ttataagcct cttttcgtgg
     1201 tcgcagtaca atatcgagct cgagtgctga gagatattta aaaaggcttt ctatcgagca
     1261 agaagcagga tgattttcga gcgctgaaat tgtcttgggc agaagaccaa cgcgttcgcc
     1321 cgcctctctt tgggtgagcc ctaacatttt ccttgttatt tggagcgaca taccgagctg
     1381 tggcagaatg cttatcaaat agtccattga aaaactccag atcttaaaag cctctgaggt
     1441 aactcaatac cttatgaggt atatcctata ataataccct aaagggtata tatcaatatc
     1501 ataccttata aggtaataat aatcagatta tggcatatct atagttttgc cagtcttagc
     1561 gtagcgttac gaagctttga tttggctact gatagcgcga ttatgaatgt attgtgtcct
     1621 taaggtagga ttcgcacaca aggcacattg caagcttggc cacacaattc agcgacatcg
     1681 tgagccgctg cttcgagatc tctctcgtca ttgcgaggcg catagataac gacagccgca
     1741 ttgtgcgctc cctcaaagag cttcgtcgcc atggagtcgc gcactgggtt agcaatggga
     1801 acaccgcgga cgccgcagat aacgcgatca tttaaagtgc ccgatattat atctttttct
     1861 atggcttttt ccttgaatag ctcaatatgc ggatccagcg cacatatgca gagtaggtct
     1921 tcgagatcga taatctgtcc ggatgtattg aagatatatt cctcattggg cgcacccaat
     1981 ctaacaagga ggctcatgcc cgctttgtcg ctatcgaaga tactcattgg gtagagatat
     2041 ctaaggcttg cgagatttac tgcatccaca aaaaaattca ccataggaaa atcgccatcg
     2101 atcgcagcct gcaaaatgta ttccgaagaa ggctttgcgc gtcctgctgg cttgtaattc
     2161 ccatttctca gcatatctcg cacagctttt ttacgcgcct cgaaatgggc tgaaggccaa
     2221 acccgaattt cgcgcagtat ctccttggca cgtgtgggaa cgtagtcggg attctgagcg
     2281 gttttgaaag acgcagtctg ccagtccaag cctttgagct ccactatagc gagtcgcacc
     2341 ttttcgaggc gggaatccgc atcgatcgca agttcgatat ccatggcgcc ttattttagc
     2401 gagcgcctgc aatagactca attgcccacc ttgcgttgac ttttttacgc tgttctgata
     2461 gattcgtgga gcgctgcata atagccgagg cgaaatgtcg ggcccttagc tcagttggtc
     2521 agagctgcgg actcataatc cgtaggtcgc tggttcaagt ccagcagggc ccaaattcaa
     2581 attgctttta gttttacttc actactg
//
LOCUS       DAOSOB010000112         2534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_40977_length_2534_cov_56.922953, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000112 DAOSOB010000000
VERSION     DAOSOB010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40977_length_2534_cov_56.922953"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1307
                     /gene="gguB"
                     /locus_tag="PLS88_08310"
     CDS             129..1307
                     /gene="gguB"
                     /locus_tag="PLS88_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOU07386.1"
                     /translation="MSELMTVLKKNVREYGMYIALAAIMAVFTILSKGIFISSRNIAN
                     LLNQTGYIAVLAVGMTLVIVIRHIDLSVGFLSGFLGAIAAIALTQWNWPLWLTILFVL
                     ALGVVAGLLTALPVASLGIPAFVASLAGWLIYRGALMLVTAATGTIIIPNEAFNAIGN
                     GYIPDILASETFLPGVHKTTLLLGAIAIVFTIFGQIADRKRKIQYKFEVSSSGLFAAQ
                     LIFISVIIGFITWILAGYNGLSWTIVIMLAVVLVYDFITRRTVLGRHIYAVGGNPEAA
                     ELSGIRVKNITFVVFASMGFLAALSGILFTARLKSATPQAGTLFEMDAIASAYIGGVS
                     AAGGVGSISGSLIGALVYMSLMNGMNLLGTDISLQYIIRGLVLLLAVIFDVMTRKQKA
                     "
     gene            1412..>2534
                     /locus_tag="PLS88_08315"
     CDS             1412..>2534
                     /locus_tag="PLS88_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU07387.1"
                     /translation="MKKFLFGALAIAILMAGCGGGGKSSVIKAGWLGSLTGDQAVWGE
                     CELNTVKMLFEEYNAAGGIEVGGKKYTLEVIGYDNKGDAQESVNATKRLTGQDKVVGI
                     IGPNASGCAIPIAPVLEKAKVPDIATVATNPKVTVQDGKVKPYNFRVCFIDPYQGAVA
                     AGFAYDRLGARTAAVLYDVSDDYSQGLREFFKINFEKKGGTIVADESFNSGDVDFRPQ
                     LSKIKAQNPDVIFMPYFFKEVSLSANQARELGIKATLLGGDGWPSDQLISMGGKAVEG
                     SYFVNHLDYADPAVQDFKNRYREKYGKEAELNGFLAHDAVLLLVEGLKKAGKPNGEAL
                     AKALEGIDVQGITGRIHISPETHNPEGKDAAILKIVNGEY"
BASE COUNT          669 a          518 c          650 g          697 t
ORIGIN      
        1 gaaatactgg gcatgagcga tcgtatctat gtcgtagcga atgggacgat tactggcgag
       61 ctcgatgcaa aatcggcaac gcaagaagcg atcatgcgcc ttgcgacaca gtactgagga
      121 ggaagcagat gagtgagtta atgactgtgc taaagaagaa tgttcgcgaa tacggcatgt
      181 atatcgccct ggcagcgata atggcagtat tcactattct gtctaagggt atattcatat
      241 catcgcgaaa tatcgctaac ctccttaatc aaacaggata tatagccgta ttggcagttg
      301 gcatgacttt ggttattgta attcggcata tagacctgtc tgtgggtttc ctatcgggat
      361 ttctaggtgc cattgccgcc atagctttga ctcaatggaa ttggccgcta tggctaacaa
      421 ttctgttcgt gcttgcattg ggcgtagtag caggacttct aactgcgctc cctgtggcga
      481 gccttggtat tcccgccttc gttgcatctc ttgccggatg gcttatttac cgaggcgcat
      541 tgatgctggt cactgcagct acaggaacca tcataatacc aaacgaggca ttcaatgcca
      601 taggcaatgg ctatattccc gatatcctag ctagtgagac atttttgcct ggagttcaca
      661 agactacgct tcttttagga gcgatcgcca ttgtgtttac gatttttgga cagattgctg
      721 atcgaaagcg caaaatccag tataaatttg aggtttcctc ttctggtcta tttgcggcgc
      781 agcttatttt tatttccgtg atcattggtt tcataacatg gattctagct gggtataatg
      841 gcttatcctg gactatcgta ataatgctgg cggttgtgct ggtatatgat tttattaccc
      901 gacggacagt gcttgggagg catatatatg ccgttggtgg aaatccagag gcggctgagc
      961 tttcgggtat ccgcgtaaag aacataacat ttgtagtatt tgcatcaatg ggatttttag
     1021 cagctctttc cggaattcta tttactgcaa ggctcaaatc ggcgactcca caggcaggca
     1081 cgctattcga aatggatgca attgcttcgg catatatcgg aggagtgtcg gcagctgggg
     1141 gagttggttc tataagtggt tctttaattg gtgctcttgt ctatatgtcg ctcatgaatg
     1201 gcatgaatct gctcggaaca gatatttctt tgcagtatat tatccgtggc ttggttcttc
     1261 tattggctgt aatcttcgat gtaatgacgc gaaaacagaa agcataagcc cagaacattg
     1321 cttctatttt gcatttatat acttgtaggt actccaatca gcgggtataa ttagcagaat
     1381 tcctgtgcat acccaaagga ggtactcgag tatgaaaaaa tttctatttg gggctttggc
     1441 tattgctatc ttaatggccg gctgtggagg cggaggcaaa tcgtctgtta taaaggctgg
     1501 gtggcttggt tcactgaccg gagaccaggc cgtatggggc gagtgcgagc tcaataccgt
     1561 aaagatgttg tttgaagagt ataatgcagc aggcggcatc gaagtaggag gcaaaaagta
     1621 cacgctcgaa gtgataggat atgacaataa aggtgatgct caagagtccg tcaatgctac
     1681 aaagcgccta acaggtcagg acaaagtagt tggcataatt ggtcccaacg cttcgggctg
     1741 tgctattcct atagctccag tcctcgaaaa ggcaaaagtg cctgatatag cgacagtcgc
     1801 taccaatcca aaggtcacag tccaggatgg caaggtaaag ccatacaatt tccgtgtctg
     1861 ttttattgac ccataccaag gtgctgtagc agctggtttt gcctatgatc gattgggagc
     1921 gcggactgct gcagttctct acgatgtaag cgatgattat tctcaaggcc ttcgagaatt
     1981 cttcaaaatt aatttcgaga aaaagggcgg tacaatcgtt gcggacgaat cgttcaactc
     2041 cggcgatgtg gacttcaggc ctcagctatc gaaaatcaag gctcaaaacc cagatgtcat
     2101 ctttatgcct tacttcttca aagaagtatc gctttcagcc aatcaggcta gagaactggg
     2161 cattaaagca acattgctcg gtggagatgg atggccctca gatcagctaa tctcgatggg
     2221 cgggaaagcg gtagaaggga gctattttgt aaatcacctc gattatgcag atccagcagt
     2281 ccaggatttc aagaatcggt acagagagaa gtatggcaaa gaggctgaac tcaatggctt
     2341 cttagcccat gatgcggttc tgcttttggt ggaagggctt aaaaaagctg gcaagccaaa
     2401 tggtgaggca ttagcaaaag cgctcgaggg catcgatgtt caaggtatta ccggccgaat
     2461 tcacataagt ccggaaactc acaacccgga aggcaaggat gctgcaatcc tgaagatagt
     2521 caatggagag tata
//
LOCUS       DAOSOB010000113         2421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_43532_length_2421_cov_82.073542, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000113 DAOSOB010000000
VERSION     DAOSOB010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43532_length_2421_cov_82.073542"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>2421
                     /locus_tag="PLS88_08320"
     CDS             17..>2421
                     /locus_tag="PLS88_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOU07388.1"
                     /translation="MEAKKKTQPKASVVVAKKPAGRSGSSASIKGSTKGHTLRSARGI
                     IALIFFIAALFLLVSMLLWSYLPTIWKPLVLPGQTLLGIFSWAAYYLPLWLAWAAALV
                     YVPKFCPRMTYLLGTSALPFVVVTGFARLSTNPSVFFEHHPSMAKVGLSSLYAALGFL
                     LAASLMVIFAGYLKLSEWLKANGFIRTRAEKKKNGEAQSFGQRLATFIKDNSLKREER
                     RKRKEEARLARAKALAEKDISINIPDVPPPDPLKSEELGGFYASGTNRAGSEMVQKDL
                     SATSVPMGSSMGSTESSLGAHSSASAAGFDTGGISFTSVPPAKPAASEEEHKRAIVER
                     ILERKAAKPYQVPIDGLLNNYPDGQYWIIDDKTRASAGVLKETLAEFGIEAEVTGIRK
                     GPVITMFEILPAHGVKISKITNLSDNIALRLAASSVRIVAPIPGKHAVGIEVPNERRA
                     IVSLRELIESEKFRQSKMEIPVALGKDIAGEVQLIDLTQMPHILIAGATGSGKSVCVN
                     SIILSILYKRTPEEVRLLLIDPKIVELKLYNDIPHLLTPVVTEAKKAFQALQYCICEM
                     ERRYSLLDNLGVRDIRSYNRRIRERNLAQEKVPYIVVIIDEFADLMQTTGKELEATLA
                     RLAAMSRAVGIHLVLATQRPSIDVITGLIKANIPSRIAFMVASKFDSRTIIDMVGAEK
                     LLGRGDMLFSGAQDPFPVRMQGAFVSEEEVERVVAHVKTLGEPEYIDEEIFVDEEESE
                     EPTLFDEDGSDPLFEKALEIVLQQGKASVSYIQRRLKIGFNRAARLVEMMEDKGIVGP
                     AQGS"
BASE COUNT          648 a          603 c          592 g          578 t
ORIGIN      
        1 aaggacttaa ttgcccatgg aagcaaaaaa gaaaactcaa ccaaaagcct cggtggtcgt
       61 ggctaaaaaa cctgccggtc gctccggttc gtcagcctcg ataaagggtt ctacaaaagg
      121 tcatactctt agaagcgcta gaggaataat agcgctcata ttttttatcg ctgcgctttt
      181 cttgcttgtt tctatgcttc tatggtcata tctgccgaca atttggaagc ctcttgtctt
      241 gccaggacaa acattgcttg gcatattttc ctgggcagcc tattatctgc cgctttggtt
      301 agcctgggcc gcagccctgg tgtatgtgcc gaagttctgt ccgcgaatga cctatctttt
      361 gggtacttcg gcattacctt ttgtcgtcgt aacaggattt gcccgtctat ccactaatcc
      421 tagtgttttt tttgaacatc atccatcgat ggccaaagtt ggcctatctt cgctgtatgc
      481 agcacttgga tttttgcttg cggcaagcct tatggttatt tttgccggct atctcaagct
      541 ttcggaatgg cttaaagcta atgggttcat aaggacacgc gcagaaaaaa agaaaaatgg
      601 agaagcccag agctttgggc agagattggc aacttttata aaggataatt cgctaaagag
      661 agaagagagg cgaaaacgta aggaagaagc gcgcctagcg cgtgcaaaag cgctcgcaga
      721 aaaagatatt tcgataaata ttcctgatgt gccaccgcca gatcctctca agtccgagga
      781 gcttggagga ttctatgctt cgggcacaaa tagggcaggt tctgaaatgg tgcagaaaga
      841 cttaagcgca acctccgtcc ccatgggatc gagcatggga tctacggaat cgtctcttgg
      901 ggcacattct tctgcttctg ctgctggttt tgacacagga ggcatttcct ttacctctgt
      961 ccctccagcc aaacccgcag catccgaaga agagcacaag cgtgccatcg tagaacgcat
     1021 cctcgagcgt aaggcagcaa agccatatca agtaccaatc gatggacttc tcaacaatta
     1081 tcccgatggg cagtactgga taatcgacga taaaacacgc gcaagcgcag gagtactcaa
     1141 agagactcta gccgaatttg gcatagaagc cgaagtgacc ggaatccgca aggggcctgt
     1201 cataacaatg ttcgaaattc tcccagccca tggcgtcaag atttcgaaaa tcacgaattt
     1261 gagcgacaat atagccctga ggctcgcggc ctcaagtgtg cgcatcgtcg cgcccatacc
     1321 tggcaagcac gcggtaggta ttgaagtacc aaacgagcgg cgcgcaatag tttcgctgcg
     1381 cgaactcatc gagagcgaga aatttcgaca gagcaagatg gaaatacccg tcgcgctagg
     1441 caaggatata gcaggcgaag tccagcttat cgatcttact cagatgcccc atattctcat
     1501 tgctggcgct acaggctcgg gcaagtcagt ctgcgtcaac tcgattatcc tatcgatctt
     1561 atacaagcgc accccagagg aagtaaggct cttgctaatc gacccgaaaa ttgtcgaatt
     1621 aaagctctac aacgatatac cacatctctt gacaccagtt gtaacggaag caaaaaaagc
     1681 atttcaggct ctgcagtact gcatttgcga gatggaacgg cgctattctc tgctcgacaa
     1741 cctcggtgta cgagacatac gctcatataa ccggcgtatt cgcgagcgca atctagccca
     1801 agagaaagtg ccttacattg tagtgatcat cgacgagttt gccgacttga tgcagacaac
     1861 aggcaaagag ctcgaagcca cgcttgcccg tcttgcggct atgtcccgcg cggttggcat
     1921 acaccttgtg ttagctaccc aacgacccag cattgatgtc ataaccgggc tcataaaagc
     1981 caatatccca tcgcgcatcg cctttatggt cgccagcaaa ttcgatagcc gcacaattat
     2041 cgatatggta ggagccgaaa aactccttgg taggggcgac atgctcttct ccggtgctca
     2101 ggatcccttc cctgtccgta tgcaaggagc cttcgtttcg gaagaagaag tcgagcgagt
     2161 tgtcgcacat gtcaagactc ttggagagcc cgagtatatc gatgaagaaa tttttgtaga
     2221 tgaagaggaa tccgaagaac ccacgctatt cgacgaagat ggttccgatc cacttttcga
     2281 aaaagccctc gaaatcgttc ttcagcaagg caaggcttcg gtgagctata ttcaaaggcg
     2341 gctaaagatc ggcttcaatc gtgcggctcg cctcgttgaa atgatggaag ataagggcat
     2401 cgtaggccca gcacagggct c
//
LOCUS       DAOSOB010000114         2375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_44764_length_2375_cov_73.448707, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000114 DAOSOB010000000
VERSION     DAOSOB010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44764_length_2375_cov_73.448707"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLS88_08325"
     CDS             complement(<1..1171)
                     /locus_tag="PLS88_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU07389.1"
                     /translation="MDKLAKQQKISQRFGLGYFMLFAMYGISSPYLQLMVRRLGYSPA
                     AVGFFLGFFELVGITGPVFIAQIADRFGKLKPFLYASAIMIIVGLVLLVPFRLPLVTL
                     FSLALLSIGIKTPIPIMDTSLLRAIEYTKEKGEKAPNYGVIRAIGSAGFVVVALIAQA
                     IPGFDSSSTATMAIALGFLALLFLLSIPSLPEIGAGQARKEKIRFSFKWLDATYLAGL
                     GVIALGRLAMAPISSFFSMYLMEELNWHAVGAMWALSATCEIPFIVLSWKLIQGRSPM
                     VAIAISSVAIVVRLLIYALFPTPLGAVLGQLLHSLCYGLFQPAAILFINLKTPPSDRA
                     TGMALYMGVGMGLPAFIGSALGGTVVEILGYRWLFASYTLFAVASIALFWRFRKEL"
     gene            1261..>2375
                     /locus_tag="PLS88_08330"
     CDS             1261..>2375
                     /locus_tag="PLS88_08330"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOU07390.1"
                     /translation="MNILDLLHTHCPSNQKKKNLLMIGGLLIGDLFAVMTSFGSGFFL
                     ENLIDMQGINFKSFVTYWPYLPAFIVMLSLLNLYPGITLSAPEKLRRFTIASFLAHAG
                     IILSLYISAKRFDQYQTAFLFSWVFSIPLFFYCRTVSESLLVRHKMWGIPAVIFGAGE
                     TGRLVVDRLLDNLKLGYIPVLLLDDNPLLAGEYRGVPIITGINLGSRIASSGEVQTAI
                     IAMPEVDHRRLGEIIVQQAMEFRRYVIIPDIIGISSLWMTVQDLDGILGLLTDQELLE
                     PRNMAIKRLMDIVLSIIGMILLLPIFIIIAILIKLDSPGPVFYRHHRLGKDSKPIEIL
                     KFRSMYNNSSQMLERLLQDSPEARTEWQSNFKLKNDPR"
BASE COUNT          690 a          540 c          507 g          638 t
ORIGIN      
        1 taagttcctt cctgaacctc caaaacagcg cgatactagc cacggcaaaa agcgtgtagc
       61 ttgcaaagag ccagcgatag cccaagattt ctaccactgt cccacccaag gcgcttccta
      121 taaaagccgg aagacccatt cctacaccca tatagagcgc catgccggta gcacgatctg
      181 aaggcggcgt tttcaaattg atgaatagaa ttgccgctgg ctggaaaagc ccatagcaga
      241 gcgaatggag aagctgaccc aaaacagcgc ctaaaggcgt cgggaaaagc gcatatatca
      301 gaagcctcac cacaatggct acagaagata tcgcaattgc caccataggg cttcttccct
      361 gaatgagctt ccaggacaac acaatgaatg gaatttcaca ggttgcagac aatgcccaca
      421 tagcgcccac agcatgccaa tttagttcct ccatgagata catcgaaaaa aatgagctga
      481 ttggagccat agctaagcgc cccagcgcta ttacgcccag ccctgcgagg taagttgcat
      541 cgagccactt gaacgaaaat ctaatcttct ctttgcgcgc ttgtcctgca ccaatctcag
      601 gcaaagaagg tatgctcagc aagaatagca atgcaagaaa acccaatgct atcgccatag
      661 tagctgtcga actcgaatca aaacccggaa tagcttgagc tattagcgct acgactacaa
      721 aaccagcaga gccaattgcc cttataactc cataattcgg tgctttttct cctttttctt
      781 tggtatattc gatggcccgc aaaagagaag tatccataat aggtatcggg gtctttatcc
      841 caatggagag cagcgcaagg ctaaagagag taaccaaagg aagccggaaa ggaactagaa
      901 gtacaagacc aacgattatc attatcgccg aagcatatag aaaaggtttg agcttcccga
      961 atctgtccgc aatctgtgct atgaacaccg gccccgtaat tcccacgagt tcgaaaaaac
     1021 cgaggaaaaa gcctaccgca gcagggctat agccgagcct gcgcaccatg agctggagat
     1081 acggtgagga aataccatac attgcgaaga gcataaagta accgagtcca aaacgctgcg
     1141 atattttctg ttgttttgca agcttgtcca taactacttg aaagaatagg gttaaggggc
     1201 agatgcgtca acatttgttg caagaatagc aaagaatatg taaaatgatt attaatggaa
     1261 atgaatatct tagatctttt gcatactcac tgtccttcca atcaaaagaa aaaaaatcta
     1321 ctcatgatag gtggacttct cataggtgat ctttttgccg tcatgacaag ttttggctcg
     1381 ggttttttct tggaaaacct tatcgatatg caagggatca atttcaagtc atttgttact
     1441 tattggcctt atcttccagc atttatagtc atgttatcgc ttctaaatct ctatcctggg
     1501 attacccttt ccgcaccaga aaagttaaga cgttttacta ttgcctcgtt ccttgcccat
     1561 gcaggaatta tcctttccct atatattagc gctaaaagat ttgatcaata tcagacggcg
     1621 ttcttgttct cctgggtctt cagtattcct cttttctttt actgcagaac tgtttcagaa
     1681 tcattgcttg taaggcataa gatgtgggga attcctgctg tcatatttgg agcaggggaa
     1741 acaggtaggc tcgttgtcga tcgccttctc gataatctca aattgggtta tatccctgtg
     1801 cttctgctcg atgacaatcc tcttcttgcg ggtgaatatc gaggcgttcc cattattaca
     1861 ggtattaacc ttggttctcg tattgcatct tcgggagaag tccagaccgc aattatagca
     1921 atgccagaag tggatcatag gcgtttaggt gaaatcatcg ttcagcaggc gatggaattc
     1981 cgccgttatg ttattattcc tgatatcata ggtatttcaa gcttgtggat gacggtgcag
     2041 gatcttgatg gaattctagg cttgcttacc gatcaggagc ttctcgagcc ccgaaatatg
     2101 gcgattaagc gcttgatgga tatagtacta agcattattg gtatgatatt gcttttgccg
     2161 atttttatca ttatcgcgat tcttataaag ctcgattctc ctgggccagt attctatcgc
     2221 catcatcgac taggtaagga tagcaaacct atcgaaatac ttaaatttcg ttcgatgtac
     2281 aacaattctt ctcaaatgct tgaaagattg ctacaggata gtccagaggc acggacagaa
     2341 tggcaatcta atttcaagct aaaaaacgat cctcg
//
LOCUS       DAOSOB010000115         2210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_49292_length_2210_cov_80.532251, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000115 DAOSOB010000000
VERSION     DAOSOB010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49292_length_2210_cov_80.532251"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1078)
                     /locus_tag="PLS88_08335"
     CDS             complement(26..1078)
                     /locus_tag="PLS88_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOU07391.1"
                     /translation="MKAALFTQFGESIDIVDVPDPIAPDDGVVLKVDATGICRSDWHG
                     WKGHDPDIVLPHVPGHELAGTIVEIGKDIKNWKPGERVTMPFVAGCGHCGPCLSGNQQ
                     VCDNQFQPGFTAWGSFAEYVAIRYGDMNLVRLPESIDAATAASLGCRFATAFRALEAQ
                     ARLHAGEWVAIFGCGGVGLSAIMVAAAMGARIIAIDIQENKLDKAKELGADIVINSRK
                     VPNIVEAIRDISQGGVQVSMDALGSPDTCFTAIASLAKRGRHVQVGLMLADHKHPAIP
                     MDLVIARELEIYGSHGIQAYRYNILLGMIEAEKLHPECLIEARLSLRQGIDFLQKMDR
                     FPGIGINVITSMQDNS"
     gene            complement(1151..1765)
                     /locus_tag="PLS88_08340"
     CDS             complement(1151..1765)
                     /locus_tag="PLS88_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOU07392.1"
                     /translation="MKELYRSDDNRCIIAVGDITNIACDAIVNAANSSLLGGGGVDGA
                     IHSAGGPEILAECRELRNSTLPNGLPPGKAVATTAGRLPAKRVIHTVGPIWHGGTHGE
                     DETLASCYRECLALAVRKQLKSIAFPAISTGIYGFPKERAAKIAWNTVQHFLANNPEA
                     TSLIVWFVFFSEKDARIFLDANELSRYLSNKVHSSAEPGKRTAV"
     gene            complement(1815..>2210)
                     /locus_tag="PLS88_08345"
     CDS             complement(1815..>2210)
                     /locus_tag="PLS88_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU07393.1"
                     /translation="LALIVDDDKDLNTILKRLLERKIEDLRVAQAYDGFEAGRIIAER
                     HPAIVILDLNLPGVDGISLCKKIRSDESFGQPAVIAITGIGEEDAQPRMMEAGADAFF
                     SKPLNFEALIAKMKELMETRKNLEKEDDT"
BASE COUNT          528 a          538 c          530 g          614 t
ORIGIN      
        1 tcttgggaat tctgtcaaaa tcttgctagg aattatcttg catcgatgtg atcacattta
       61 tgccaatacc tgggaaccga tccattttct gcaaaaaatc gatgccttgg cgtagggaaa
      121 ggcgtgcttc gatgaggcac tcagggtgta gcttttctgc ttcaatcatt ccaagaagaa
      181 tattataacg gtatgcttga ataccatggc ttccatagat ttcaagttct cgcgcaatta
      241 cgaggtccat agggattgcg gggtgcttat gatcagcgag catgaggcct acttgcacat
      301 ggcgaccgcg tttggcaagg cttgcaattg cggtgaaaca agtatctggg ctacctagag
      361 cgtccattga aacctgtact ccgccctggc ttatatcgcg aattgcttcg acaatattag
      421 gcacttttcg gctatttatg acgatatcag caccgagctc cttggcttta tcgagcttat
      481 tttcttgaat atcgatcgct atgatccgtg ctcccatcgc agccgcgacc atgatcgctg
      541 aaaggccaac gccgccacat ccaaagattg caacccattc tccagcatga agcctggctt
      601 gtgcttccag cgctcggaat gctgtagcaa agcgacagcc gagacttgcg gcggtcgctg
      661 catcgatact ctcaggcagg cgtacgagat tcatatctcc ataccgaata gcgacatatt
      721 ctgcaaaaga accccaagcg gtgaatcctg gttggaactg attgtcgcag acttgctgat
      781 tgccgctgag gcaaggccca cagtggccac agcctgccac gaagggcatg gtgactctct
      841 ctcctggttt ccaattcttg atatccttgc caatttcaac aatagtgcca gcaagttcgt
      901 gtcctggtac atgaggaagc acgatatcag gatcgtgtcc cttccaccca tgccaatcgc
      961 tacggcagat gccagttgca tcgactttga gtacgacgcc atcgtcagga gctataggat
     1021 cagggacatc aacgatatcg attgactctc cgaattgcgt gaatagcgcg gctttcatag
     1081 ctttatttcc tttcgttcat tggctcaggt ctgcgatcat ctggattgta ctctgcttaa
     1141 gtgtactctg ctaaaccgca gttcgcttac cgggttccgc tgaactatgt actttattgg
     1201 atagatatcg ggatagctca tttgcatcga gaaaaatgcg tgcatctttc tcggaaaaaa
     1261 atacaaacca gactatgagg gaggtagctt cagggttgtt cgcaagaaaa tgctgaaccg
     1321 tgttccaggc aattttagcg gcacgttctt tggggaatcc atagatgccg gttgagatgg
     1381 caggaaaggc aatcgacttg agctgcttgc gcacagcgag agcaaggcat tctctgtaac
     1441 aggatgcaag ggtttcatct tcgccatgtg tacctccgtg ccaaatcggg cctactgtat
     1501 ggatgacacg tttggcggga aggcggcctg cagtagttgc cactgctttg cctggcggca
     1561 gcccattggg caaggtgcta tttcgcaatt cgcgacattc ggcgaggatt tctggacctc
     1621 ccgcactatg gatagctcca tcaacaccgc caccgcctaa taggcttgag ttagctgcat
     1681 tgacaattgc atcgcaggca atattggtta tgtctccgac tgcgattatg cagcggttgt
     1741 catcagatct atatagctct ttcataatag aaatctatcc ttttagacat gaactctttt
     1801 tagcatagtg ttctttaagt atcatcttcc ttttccaaat tcttgcgggt ttccatgagt
     1861 tctttcattt ttgcgatcaa ggcttcgaaa ttcaatggct tagagaagaa tgcatcggca
     1921 ccggcttcca tcatgcgagg ctgagcatcc tcttctccaa ttcctgtaat agcaattacc
     1981 gctggctggc caaaggattc atcggatcgg atttttttac agagcgaaat gccgtcgact
     2041 ccaggaagat ttaaatccag gatgactatg gctggatggc gttctgctat aattctgcct
     2101 gcttcaaagc cgtcgtaagc ctgtgcaacg cgtaggtctt caatttttcg ctccaggagg
     2161 cgctttaaga tcgtattgag atctttatcg tcgtctacaa ttagcgcaag
//
LOCUS       DAOSOB010000116         2132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_51821_length_2132_cov_98.582571, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000116 DAOSOB010000000
VERSION     DAOSOB010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51821_length_2132_cov_98.582571"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..242
                     /gene="rpmC"
                     /locus_tag="PLS88_08350"
     CDS             36..242
                     /gene="rpmC"
                     /locus_tag="PLS88_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOU07394.1"
                     /translation="MKNSFKDLRLEELLIKRDELRKKYFDLRFQMVVGHVENPVEKRN
                     LRRQIARIETRIAEAQRSSAAKKA"
     gene            282..533
                     /gene="rpsQ"
                     /locus_tag="PLS88_08355"
     CDS             282..533
                     /gene="rpsQ"
                     /locus_tag="PLS88_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOU07395.1"
                     /translation="MTEGKLVLQGVVVSDKNDKTIVVEIIMRKLHPLYRKYVNRTKRV
                     KAHDEHNEAHVGDTVSVVECRPMSREKRWRLVQIVERAK"
     gene            550..918
                     /gene="rplN"
                     /locus_tag="PLS88_08360"
     CDS             550..918
                     /gene="rplN"
                     /locus_tag="PLS88_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOU07396.1"
                     /translation="MIQVESMLNVADNSGAKTVQCIKVLGGTRRRYASVGDIIVVAVK
                     TALPNSAVKKGSIEKAVVVRTHKEYRRPDGTYIRFDDNACVLIDANRNPKGKRIFGPV
                     ARELREKDYMKIISLAPEVL"
     gene            930..1250
                     /gene="rplX"
                     /locus_tag="PLS88_08365"
     CDS             930..1250
                     /gene="rplX"
                     /locus_tag="PLS88_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOU07397.1"
                     /translation="MPNAKYKLRKEDLVQVIAGKERGKQGKVLKIDRENGRVIVSGLN
                     MVKKAMKKKSQTDRGGIVAVEAPIHISNVMIVCKKCNKPVRAGYKIIDGKKKRVCRKC
                     GEVL"
     gene            1250..1885
                     /gene="rplE"
                     /locus_tag="PLS88_08370"
     CDS             1250..1885
                     /gene="rplE"
                     /locus_tag="PLS88_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOU07398.1"
                     /translation="MAAQTTHTAQKAATVHTAQKTETAQSAQAKAYVPRLKKLYREKV
                     ISELFDELGYSSTMQVPRLEKIVVSMGVGEARDNKKLLDAAINDLETIVGQHAVKTKA
                     KKSIANFKIRKGQEIGARVTLRGNQMWEFLDRLMNVALPRVKDFRGVNPNGFDGHGNY
                     TLGLTEQIIFPEIDFDKIEKVMGLSVSIVTTAETDMEAKSLLAKLGMPFRK"
     gene            1897..2082
                     /locus_tag="PLS88_08375"
     CDS             1897..2082
                     /locus_tag="PLS88_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002557082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOU07399.1"
                     /translation="MARKAMFLKAMKTPKYSTRLVRRCKICGRSRGYMRKFDMCRICF
                     RKLASEGKIPGVTKSSW"
BASE COUNT          654 a          442 c          580 g          456 t
ORIGIN      
        1 aagtttgcga tccgcagcga gatggagtga tcaccatgaa aaactcattc aaggacctaa
       61 gactggaaga attgctcatc aagcgcgatg agcttcgaaa gaaatacttt gacctgcgtt
      121 tccaaatggt ggtcggccac gtagagaatc cggtagagaa gagaaatctt cgccgccaga
      181 ttgcgcgtat agaaactaga attgccgaag cccagcgcag cagtgccgcg aagaaggcat
      241 aattcttcag gagaaagaac gtggatgcga atatccaaaa gatgaccgag ggcaagcttg
      301 ttcttcaagg cgtcgtagtt tcggacaaga atgataagac tattgttgta gaaattatta
      361 tgcgcaagct acaccctctc tacagaaaat atgtcaatag gacgaagcgc gtaaaagccc
      421 acgatgagca caacgaagcg catgtcggcg ataccgtttc agtggtcgaa tgcaggccaa
      481 tgtcgcgaga aaagcgatgg aggcttgttc aaatcgtcga gcgggcaaaa taacgagcgg
      541 gagtaggcta tgattcaggt agaaagcatg ctgaatgtcg ccgacaattc aggtgctaag
      601 actgtccagt gtatcaaggt gctaggtggt acacggcgta ggtatgcgag cgtcggcgat
      661 atcatcgtag ttgcagtaaa gacagccctg cccaactcgg cagtcaagaa aggatcgata
      721 gaaaaagccg ttgtcgtgcg tacacataaa gagtacaggc ggccagatgg cacatacatc
      781 aggttcgatg acaatgcttg cgttctaatc gatgctaaca gaaacccgaa aggcaagcgc
      841 attttcggac cggttgctag ggaactaaga gagaaagatt atatgaagat catctctctc
      901 gctcctgagg tcctatgagg agtctgaata tgcctaatgc gaagtataaa cttcgaaaag
      961 aagaccttgt tcaggtaata gctggcaagg agcgggggaa acaggggaag gtcctcaaga
     1021 tcgatcgtga aaatggtcgc gtcatcgtgt ctggtctcaa tatggtaaag aaagccatga
     1081 aaaagaaatc ccagacggat cgtggcggca tagtagccgt cgaggcaccg attcatatat
     1141 cgaatgtgat gatcgtatgc aagaagtgca ataaacccgt tcgtgccggc tacaagatca
     1201 tcgatggcaa aaaaaagagg gtttgtagaa aatgcgggga ggtgctttga tggcagctca
     1261 gactacacat acagcacaaa aggctgcgac agtacatacg gcgcaaaaga ctgaaacagc
     1321 acagtcagcg caagctaagg cctatgttcc ccggctcaag aagctctatc gcgaaaaggt
     1381 gatatcagag ctcttcgatg agcttggtta cagctcaaca atgcaagtgc ctcgtctcga
     1441 aaagatcgta gtatcgatgg gtgtcggcga ggcgcgcgat aataagaagc ttctcgacgc
     1501 ggcaataaac gacttagaga ctattgttgg tcagcacgct gtcaagacaa aagctaagaa
     1561 atccatcgcg aacttcaaga ttcgaaaggg tcaggagata ggagcacgag taacattgag
     1621 aggcaaccag atgtgggagt ttctcgacag gttaatgaat gttgcgcttc ctcgtgttaa
     1681 ggattttcgt ggtgtaaatc cgaatggatt cgatggccat ggaaattaca cccttggtct
     1741 gaccgagcag attatttttc cggaaatcga tttcgacaaa atcgaaaagg ttatggggct
     1801 cagcgtctcg attgtgacaa cagccgagac agacatggag gcaaaaagcc ttcttgccaa
     1861 gttgggcatg cccttcagaa aataaggaag gcatacatgg ctcgaaaagc aatgttttta
     1921 aaggcgatga agacgccgaa atactccact cgtctggttc gcagatgcaa aatctgcgga
     1981 aggtctcgcg ggtatatgag aaaattcgat atgtgccgca tttgcttccg gaagcttgct
     2041 tccgaaggca aaataccagg cgtcacaaaa tcgagctggt aggagggtgc aatgagtgta
     2101 tctgatccca ttgcggacat gctgaccaag at
//
LOCUS       DAOSOB010000117         2124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_52089_length_2124_cov_72.163364, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000117 DAOSOB010000000
VERSION     DAOSOB010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52089_length_2124_cov_72.163364"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1307)
                     /locus_tag="PLS88_08380"
     CDS             complement(66..1307)
                     /locus_tag="PLS88_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU07400.1"
                     /translation="MPKLKYVIRHEFKLTAANKVFIILTIIGPFLIFAVTVLPTLLAQ
                     SPGTVASDKPLALFGAPPSVEQALSAAFSTMKLKVERVEDIEAAKASVVDGSRLALLE
                     LEAGWPDTGKAIWYSKSSTEINVYSTASAVLEAVAREERIKESGIDPALAAKLLSKPS
                     FDVIKLGKGGEEKVQGQGDQAFIETLMTVLVFIMLLYMTILLYGQMIGRSVVTEKTSK
                     TVEIMLSSVTSRDLMIGKILGLGLAGILQYAVWISMAFLLNRFLGPLFGLALPVAVSM
                     QNMIWLFVFFILGFFLYSSAYAALGAASEDEQNLGQVAWPLLIFLIIPMVMLNTLVTS
                     PDSTIVVIMSIFPLTSPIVMLSRILVSSPPAWHLMLCIGILVASVAGMMIFAGRVFRV
                     GILMTGKRPKLKEVLRWAKVK"
     gene            complement(1300..>2124)
                     /locus_tag="PLS88_08385"
     CDS             complement(1300..>2124)
                     /locus_tag="PLS88_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU07401.1"
                     /translation="EIFGLIGPNGAGKTTTIRMIMNIIAPDSGMIRFDGAPLSEADKD
                     RIGYLPEERGLYRKAKLGEVLEYLAAIKGAKPADFQPAIDSWLARFDLSEWKNRKVSD
                     LSKGMAQKAQFIAAVAHNPSVVLFDEPFSGLDPMAQDELLAVMVELKQKGATVLFSTH
                     IMEHAEKICERILLMNKGKAVVYGSMADIKAQYGRNAVQLEFDGDASFVQSLDFVDSV
                     VSYPRWIEVELKEGTDSDLLFKALAGRIKVRRFETVAPSLHKIFIQLVGGEEDENA"
BASE COUNT          545 a          552 c          502 g          525 t
ORIGIN      
        1 ctcgtgaaag ccacctttat ctcattttta actttgcctc atttgtgcct tacgtaagcc
       61 ctaagctatt tcacctttgc ccagcgcaac acctctttga gcttaggcct tttgcccgtc
      121 atgagtatgc ctacccggaa aactcttccc gcgaatatca tcatgcccgc gaccgaggca
      181 acaaggatgc caatacagag catcagatgc catgccggag gtgacgaaac aaggattctg
      241 gaaagcatca cgatagggct cgtgagcgga aagatagaca tgataaccac tatagttgaa
      301 tctgggcttg tcaccagcgt atttagcata accataggta tgatcaagaa aatcaataga
      361 ggccaagcga cctgccccag gttttgctca tcctcgcttg cagctcccaa tgcggcgtat
      421 gccgaagaat atagaaaaaa acctagaata aagaaaacga agagccatat catattctgc
      481 atagataccg ctacaggcaa agccagcccg aagagcggcc ccagaaagcg attgagaaga
      541 aatgccatcg atatccaaac cgcatactga aggattccgg caagccctaa gcccaatatc
      601 ttgcctatca tgagatctcg actcgtcacg ctcgagagca tgatctccac ggtcttcgag
      661 gtcttctcgg ttacaacgga gcgtcctatc atctgtccat agagcaatat ggtcatatag
      721 agcagcatta taaataccag aactgtcata agtgtctcga taaaggcctg gtctccctgg
      781 ccctgaacct tctcttcacc cccttttccc agctttatta catcgaaaga aggcttggac
      841 agaagttttg cagccagagc tgggtcaatt ccgctttcct ttattcgctc ttcgcgcgct
      901 acagcctcta agacggccga agcggtgcta tatacattta tttcagtgct gctctttgag
      961 taccagatgg ctttccctgt gtcaggccat cctgcttcga gttccagcaa agcaagtcga
     1021 gagccatcga caaccgaagc tttagccgct tctatgtctt cgactcgctc gactttgagc
     1081 ttcatggtgg aaaaggcagc tgaaagagct tgttcgaccg aaggtggagc tccgaagagg
     1141 gcaagaggct tgtcagatgc caccgtgcct ggagattgag cgagcaaagt aggtaggaca
     1201 gttaccgcaa agatgagaaa tggcccaatt atggtgagga ttataaaaac cttattagcc
     1261 gcggtcaact tgaactcatg gcgtataaca tattttagct taggcatttt cgtcctcctc
     1321 acccccaacc agttggatga atatcttgtg caaggatggg gctactgttt caaagcgcct
     1381 aaccttgatc ctacctgcga gagccttgaa tagaaggtct gaatcggtac cttccttgag
     1441 ttcgacctca atccagcgcg gataggacac aaccgagtcg acaaagtcaa ggctctgcac
     1501 aaaagaagca tctccatcga attctagctg aaccgcattt cgaccatatt gagccttgat
     1561 atccgccatt gagccataga ccactgcttt gcccttattc ataagaagaa ttcgctcgca
     1621 gatcttctcc gcgtgctcca tgatatgggt ggaaaagagc acagttgcgc ctttttgttt
     1681 gagttcgacc atcaccgcca aaagttcatc ctgcgccatt ggatcgaggc cagagaatgg
     1741 ctcatcgaat aggacgaccg atgggttatg ggcgactgct gcgatgaatt gcgccttttg
     1801 agccatacct ttggacaggt cggatacttt gcgatttttc cactcggaaa ggtcgaatcg
     1861 tgcgagccat gaatcgatcg caggctgaaa atcggctggc ttagctccct tgatcgcggc
     1921 gaggtattcg agcacctcgc ccagcttggc tttgcgatat agccctcgct cttcgggcag
     1981 gtagcctatg cgatccttat ctgcttcgga taagggcgct ccatcgaagc gaatcatgcc
     2041 tgaatcaggc gcgattatgt tcattatcat gcggatagtt gtggtcttgc ccgccccgtt
     2101 ggggccgatg agaccgaaga tttc
//
LOCUS       DAOSOB010000118         1994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_56754_length_1994_cov_79.319237, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000118 DAOSOB010000000
VERSION     DAOSOB010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56754_length_1994_cov_79.319237"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..487)
                     /locus_tag="PLS88_08390"
     CDS             complement(11..487)
                     /locus_tag="PLS88_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOU07402.1"
                     /translation="MVAGDIEFSDELQTFVEEWSKKEGGLVMMLHRIQNEYGFIPRAA
                     AEKLSLISGIPLARIYGVITFYHFFKTEKPGKHRIAVCMGTACYLKGGQELLEETRSI
                     LSVEPGEVTDDGIFSVDEVRCLGCCGLSPVIMIDDEVYGKLTKEQLPSIIAKYRER"
     gene            776..>1994
                     /locus_tag="PLS88_08395"
     CDS             776..>1994
                     /locus_tag="PLS88_08395"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOU07403.1"
                     /translation="MKSGDTVGAYVLEQRLGKGVTASTWLARSTGSVTKLNVQPESEL
                     DNSTDAREAGPLTAISAQAYVLKIFDIAETSSWKPLDQFKREADILRTLDHPRIPKYI
                     ESFETAIDGKFLFVLVMEYIQGDTIEAYIAAGKKFTEEQIEALLAELADILAYLGSLR
                     PPIVHRDINPRNLIIRPDGQIVLVDFSGVQDAIRTALYPGATLVGTAGYIPMEQVAGR
                     ATHRSDLYGAATTTVFMLTGRNPSELPMRNLKIDLSGLLYMSPRLSYVLDNWLDPDPD
                     RRTLSAVKAAEILRGKVALSLETRANLDAKTETTSAENNQNGAGPFWNGTFSRDSFLG
                     EILHNLEEKTEQKAYPSELPSGSLLTIQNLEPGLYIKMPKRSNTSMRGGTVFPIAWMG
                     FVFFWTTMTLRMHA"
BASE COUNT          574 a          478 c          423 g          519 t
ORIGIN      
        1 tgcatcgcac tcagcgctct cggtattttg caataatgct tggtagctgt tcctttgtta
       61 gcttgccata tacttcatca tctatcatga taactggaga gagtccacag caaccaaggc
      121 atcgcacttc atctacacta aagataccat cgtctgtaac ttcgcctggt tctaccgaca
      181 agatagatcg agtttcctcg agtagttcct gaccgccttt gaggtaacaa gccgttccca
      241 tgcacactgc aatgcggtgc ttgcccggtt tttctgtttt gaagaaatga tagaacgtaa
      301 taacgccata tattcttgca agcggaatac cagaaattag cgagagcttc tctgctgcag
      361 cccgcggaat aaaaccgtat tcattctgga tgcgatgcag catcattaca agtccgcctt
      421 ccttcttgct ccattcttcg acaaatgttt gcaactcatc tgaaaactcg atgtctcctg
      481 cgaccatcct gtagctcctc ttagataata ttaggaaatc tgaatataga attatcgata
      541 aatcagtaac gaatatatga tacagctgaa accgaaaaaa ctcaagtcct ttttatgggt
      601 aaatcagacc ttgggataga tgacgaaagt ctttatttga attatctttc tcttagagaa
      661 gcaagcaatg gcgaatattc ataagcatgt aagcatttat aacctcaaaa aaacaagcta
      721 tttcgctcat tgatatgaaa agccatatcg atataaaaaa ccataaggaa gaagagtgaa
      781 atctggagat actgtaggcg cttatgttct cgagcaaaga ctgggcaagg gtgtaacggc
      841 atctacatgg cttgctaggt caaccgggtc tgtaaccaag cttaatgttc aacccgagtc
      901 agagttagat aacagcaccg atgctcgtga ggccggccca cttactgcca tttctgccca
      961 agcttatgtg ctcaaaattt tcgatatcgc cgagaccagt tcctggaagc ctctagatca
     1021 gttcaaacgc gaagccgata tcctccgcac cctcgatcat ccgagaattc ccaaatacat
     1081 agaaagcttc gagacagcaa tagatggaaa attccttttc gtgcttgtaa tggaatacat
     1141 ccaaggtgat acaatcgaag catatatcgc agctggcaaa aaattcaccg aagagcaaat
     1201 cgaagcattg ctcgccgagc ttgcggacat actggcgtac ctagggagcc tacgcccgcc
     1261 tattgtccat cgagatatca atcctagaaa tcttataatt aggccagatg gacaaatagt
     1321 gctagtcgat ttttccggag tccaggatgc cattcgcaca gccctttatc ctggcgcaac
     1381 acttgtgggc actgctggtt atatcccaat ggaacaagtg gctggtcgag ctacccaccg
     1441 ttctgacctc tatggcgctg ccacaacaac ggttttcatg cttacggggc gaaatccttc
     1501 tgaattgccg atgcgaaact taaaaatcga tctctccgga cttctgtata tgtctccgag
     1561 gctttcctat gtgttagaca actggcttga cccagatcca gacaggcgca ctctgtctgc
     1621 agtaaaggcc gcagagattt tgaggggcaa ggttgctctc tccttggaaa ctcgtgcaaa
     1681 tctagatgca aaaacagaaa cgacatctgc tgaaaacaac caaaacggag cgggaccttt
     1741 ttggaacgga accttttctc gcgattcatt cttgggagaa attttgcaca atctggaaga
     1801 gaaaaccgaa cagaaagcct acccttcgga attgccttcg ggcagcctac taacaattca
     1861 gaatctagaa cccgggctct atataaaaat gccaaaaaga agcaatacca gcatgcgagg
     1921 aggcacggtc tttcctattg catggatggg ttttgttttt ttctggacca cgatgacctt
     1981 gcgcatgcat gcgc
//
LOCUS       DAOSOB010000119         1907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_60450_length_1907_cov_70.873110, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000119 DAOSOB010000000
VERSION     DAOSOB010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60450_length_1907_cov_70.873110"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1853)
                     /locus_tag="PLS88_08400"
     CDS             complement(<1..1853)
                     /locus_tag="PLS88_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOU07404.1"
                     /translation="MELIDLAGNWNLIRFSDGTNRPIHIPGDIYSALIVSGELPDPYW
                     GENELLAQWPGKEDWIIERSFFISTDFMAAPNIRLCADVLDTIAEVYINKILVGTSNN
                     MFRPFEAEVRSVLHLGENNIQVIIRSPVQTALRESQRLPYPIPASIYPISSPHRNLIR
                     KAQCMSGWDWGPCLMTGGIYDGIRLVASDGPFIRSIQCHTSALGGFTSRNDCGPDFSL
                     RISAEVEILEPMKVAVSFYIYGKKVIVEQNCEQGNTVISTNLVIQNPRLWWPHGHGEA
                     ALYDLVVTATAVTDMAVTDMAAQASNRPQPHVIHKSIGFRSLEVISQEDSIGRGFKFL
                     VNGKEVFAKGANWIPADALPSRWTRERIEHLLDSAISANMNCLRVWGGGRYESDDFYE
                     LCDKKGILIWQDCMFSCALYPSSREFLSNVEAEITAQVKRLSDHPCIALWCGNNEALG
                     AITWYEESKKNPARYIVDYDRLTEGVLGKTIRRLDPDRCFWPSSPSAGPDDFSDNWHS
                     DERGDMHFWSVWHEGKPFSAYLDVQPRFCSEFGFQALPSISTIASFAPREEWNISAPA
                     FEAHQKNPQGNSIILESLLRYFRMPCGFHELLYLSQVQQAMAIRTAVEFWR"
BASE COUNT          499 a          459 c          442 g          507 t
ORIGIN      
        1 ctgcgccaga attccactgc tgtgcgaata gccatagcct gctgtacttg agagagatag
       61 agaagctcat gaaagccaca tggcatgcgg aagtatcgaa gcaagctttc tagaattatg
      121 ctgttccctt gtggattctt ttgatgggct tcgaacgccg gtgcacttat gttccattct
      181 tctctaggag caaagctagc gatagtcgat atcgaaggca gggcttgaaa accgaattca
      241 gagcaaaaac gaggctgaac atcgagatat gctgagaatg gcttgccttc atgccacact
      301 gaccagaagt gcatgtctcc gcgctcatcc gagtgccaat tgtctgagaa atcgtcggga
      361 cccgcagatg ggctggaagg ccagaagcaa cggtctggat caagccttct aatcgttttt
      421 ccaagaacgc cttcggtaag cctatcataa tccacaatat atcgcgcagg attcttcttc
      481 gactcttcat accaagttat ggcaccaagc gcctcattgt ttccgcacca gagagctatg
      541 caaggatgat ccgaaagtct ttttacctgt gcagtgatct cggcctccac attggatagg
      601 aattctcggc tcgaaggata gagggcgcag gagaacatgc agtcctgcca gatcaaaatg
      661 cctttcttat cgcacaattc atagaaatca tcgctctcat agcgcccacc tccccagacg
      721 cggaggcagt tcatattcgc gcttatggcg gaatcgagca gatgttcgat acgttctctt
      781 gtccatctcg atggcagggc atcggccgga atccagtttg cacccttggc gaatacttct
      841 ttgccattta cgagaaactt gaagcctctt ccaatgctat cttcttgcga aatcacttct
      901 agcgatctaa agccgatgct cttatgtatc acatggggtt gagggcgatt tgaagcctgg
      961 gcagccatat cagtgacagc catatcagtg acggccgtag ctgtgaccac tagatcgtag
     1021 agagcagctt caccatggcc atgcggccac catagtctgg ggttctgaat taccagatta
     1081 gtgcttatga ctgtattccc ctgctcgcaa ttctgttcca ctataacttt cttgccatag
     1141 atatagaaag agacagcaac tttcatgggc tctagtattt cgacttctgc tgagattcga
     1201 agcgaaaagt ctggcccgca atcattacgg cttgtaaagc ctcctagagc cgaggtatgg
     1261 cattgtatag agcgaataaa tggcccatct gaagccacaa ggcgaatgcc atcatatatt
     1321 ccaccagtca ttaggcatgg accccaatcc catcccgaca tgcattgcgc cttacgtatt
     1381 agattgcggt gtggagatga aatggggtag atcgaggctg gtatgggata aggaaggcgc
     1441 tgtgattcac gcaaagctgt ttggacaggt gagcggataa taacttgtat gttattttcc
     1501 cctaaatgca gaactgagcg gacttcggct tcgaaaggcc gaaacatgtt gttgctggtt
     1561 cccaccaaga tcttatttat atatacttca gcaattgtat cgaggacatc ggcacagagc
     1621 cttatgttgg gcgctgccat aaaatccgtg gaaatgaaaa aggatcgctc gataatccaa
     1681 tcttcttttc caggccattg agccaacagt tcattttctc cccagtaagg atcaggcaat
     1741 tctcccgaaa ctatgagagc tgaatatata tctccaggta tatgaattgg ccgatttgta
     1801 ccatcggaaa acctgataag attccaattg ccagcaagat cgatcagttc cactttttac
     1861 ctcctattta tttctgcgtc tcctattgaa ttctgaaaga tttttat
//
LOCUS       DAOSOB010000120         1804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_65350_length_1804_cov_59.016581, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000120 DAOSOB010000000
VERSION     DAOSOB010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65350_length_1804_cov_59.016581"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..866)
                     /locus_tag="PLS88_08405"
     CDS             complement(120..866)
                     /locus_tag="PLS88_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOU07405.1"
                     /translation="MRICIYENAIDLGKHSAMMVSMHIRRALQEKGECSIILATGTSQ
                     LAMLDSLVKDNDIDWNKVEVFHLDEYVGIPDTHKASFRKYLRERFAERVSGLRAFHYI
                     QADANDLSVEIARLSDLISSKNIDVACIGIGENGHLAFNDPPANLDTQEPYLIVQLDE
                     ACRRQQVGEGWFDDISEVPAQAISMSIPQILKSECIVCTVPDKRKAEAVSMAITAPID
                     PMHPCASLRMHKNCYLMLDYSAASLICPLS"
     gene            945..1775
                     /locus_tag="PLS88_08410"
     CDS             945..1775
                     /locus_tag="PLS88_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU07406.1"
                     /translation="MRIIEQFLQSKTGVEETCEDGFVITDCFAAVIDGVTNKNAVQLD
                     FGAPGKIAMELIKKGIGELSPQLSGPDAFKSINKVIYDSYCNLGLLETMKKNPSQRFT
                     ASCVIYNSVRRELWFLGDCMALANGTTYQFSKEADKLFSDLRSFLIHIELSQGTTEEQ
                     LLEHDVCREQILDFIIRQTFLQNSSYNCPFTYYVLDGISEEAPRHTVVIPVEEKTTEI
                     VLASDGYPALYPTLEQTEAYLTYILEIDPLCYREFRSTKGLVKGNVSFDDRAYVRLAI
                     "
BASE COUNT          500 a          369 c          395 g          540 t
ORIGIN      
        1 agacaacctg ttcgacagta agatcttttg cgctaaaatc gattcctcca tatccgttga
       61 cttgcaaatc tattagggta gcataggaat tcatagcctt gatggtcata atgatatact
      121 catgacaaag gacaaatcag ggaagccgca gaataatcta gcatcaaata gcaattttta
      181 tgcattctga gcgatgcgca gggatgcatt ggatctatcg gagcggttat tgccatggac
      241 acagcctcgg cctttctttt atccgggact gtgcaaacaa tgcattcact cttcagtatc
      301 tggggaatgc tcatagaaat agcttgtgcg ggaacctcgg agatatcatc gaaccatcct
      361 tctccgacct gttgccgtct gcatgcttca tcgagctgaa ctatgagata tggctcttgc
      421 gtatctaggt ttgctggagg atcgttaaag gcaagatgcc cattttctcc aatgccaatg
      481 catgcgacat cgatgttttt tgagcttatt aaatcagaaa gtctagctat ttcaacagaa
      541 agatcattgg catcggcttg aatgtagtga aaagctcgca atccggacac tctctcggcg
      601 aatctctctc gcaaatactt gcgaaaactt gccttgtgcg tatcgggtat gcctacatac
      661 tcatctagat gaaaaacctc tactttgttc cagtcgatat cattatcctt tactagtgaa
      721 tccagcatag ctaattggct tgtgccggtt gctagaatga tagaacactc tcccttttcc
      781 tgcagtgccc tacgaatatg catagaaacc atcattgccg aatgtttgcc tagatcaata
      841 gcgttctcat atatacaaat tctcattggc tctttcctcc tcagttttgt tctgttattg
      901 taatttatta tatatgtgaa aggaaggaag ctaaggagcc gatgatgcgg ataatagagc
      961 aatttctcca atctaaaaca ggagtggagg agacttgcga ggatggattt gtcataaccg
     1021 attgttttgc agcagtaata gatggggtga ccaataaaaa tgccgtgcag ttagacttcg
     1081 gtgctccagg gaaaatcgct atggagctga taaagaaagg cattggagag ctatcgccac
     1141 aattatcggg acctgacgct tttaagagca tcaataaagt tatttatgat agttattgta
     1201 acttgggatt attggaaacc atgaagaaga atccttctca gcgtttcact gcttcctgtg
     1261 tgatttataa ttcagtgcgg cgtgagctgt ggtttctagg ggattgcatg gctttagcca
     1321 atgggacgac ataccagttt tctaaggaag ccgataagct tttcagcgat ctgcgttcct
     1381 ttcttatcca tatcgagcta tctcaaggta caacagagga acagcttttg gagcatgatg
     1441 tatgccgtga acagatactt gactttataa taagacaaac ttttctgcag aactcatcgt
     1501 acaattgccc ttttacgtat tatgttctcg atggaataag cgaagaagcg cctagacata
     1561 cagtggttat tcctgttgag gaaaagacga ccgagatagt ccttgcaagc gatggatatc
     1621 ctgctcttta tcctacactt gaacagacag aagcctatct tacatacatt cttgaaattg
     1681 atcctctttg ctatcgtgag tttcgtagta ctaagggctt ggtaaagggg aatgtatctt
     1741 tcgatgacag ggcctatgtg cgtctagcaa tatagtcatc gatttagccc attttgaagc
     1801 tatc
//
LOCUS       DAOSOB010000121         1730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Rectinema sp. isolate AMR_MDS_2356
            NODE_69364_length_1730_cov_84.566567, whole genome shotgun
            sequence.
ACCESSION   DAOSOB010000121 DAOSOB010000000
VERSION     DAOSOB010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864423
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rectinema sp. (wastewater metagenome)
  ORGANISM  Rectinema sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Rectinemataceae; Rectinema.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,684
            CDSs (total)                      :: 1,641
            Genes (coding)                    :: 1,634
            CDSs (with protein)               :: 1,634
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Rectinema sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69364_length_1730_cov_84.566567"
                     /isolate="AMR_MDS_2356"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1937017"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..511
                     /gene="pgsC"
                     /locus_tag="PLS88_08415"
     CDS             80..511
                     /gene="pgsC"
                     /locus_tag="PLS88_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HOU07407.1"
                     /translation="MASTSIVVSIVLGFFFGELTGWLTGGLVAPGYLALYIDQPLRII
                     MTYIAALLAFFLVKLLSQISILFGRRRFMAFILAGICMGALLDAIVRQIPPSGSDLRA
                     IGYVVPGLIANDIWKQGFWRTLLGSLVITLATRVVLLLILV"
     gene            520..1725
                     /gene="pgsW"
                     /locus_tag="PLS88_08420"
     CDS             520..1725
                     /gene="pgsW"
                     /locus_tag="PLS88_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOU07408.1"
                     /translation="MKNSIAKQRQNPALFVRVLIALASLALGLVATELLAHNEPHPAA
                     EAMNKAARRMAQAEAVVAKKRLAMGLPMDPALDPYQTGLIGAESSILTTTVGDIVSKR
                     TSTNTAFAALIVRYFHDIGLESGDRIAVGSSGSFPAILIAVLSACAETGVEPIVIASI
                     GASEYGANIEGFSNAEIMAACRDAGVLPYMPIAISPGGEDDLGISSMYRLEPSDELAK
                     YAQRVAKSLDIPLIQSPDLESSFRAHLEVYEKASPIKAFVNIGGADVNFGSGPASLKL
                     KPGLIVSSARTRGTDLGKLHKQVPRDPKKSARQETSSAGTMDANIGLDAQAGLIAYYM
                     AKGLPVLHFLNIKGLAVQSGIPVDGDPRAPLPSSIVTMRIKPSWPLLISLFLAGTALA
                     IRKPLRYYP"
BASE COUNT          433 a          402 c          427 g          468 t
ORIGIN      
        1 cagttacctc agccgagggg gcacattcag ttgcttcgcc tgaaaaatcg caatccgcta
       61 attcgacagg agaaagataa tggcaagtac atcgattgtt gtttcaatag tgctcggatt
      121 tttctttggc gaattaacag gatggcttac aggtggactc gtggctcctg gatacctcgc
      181 gctatatatt gatcaacctt tgcgaataat aatgacatat atagcggccc ttttggcgtt
      241 ctttttggtc aaattgcttt cgcagataag cattttgttt ggacgaaggc gttttatggc
      301 cttcattctg gctggcattt gtatgggcgc tttactcgat gctattgtaa gacagattcc
      361 cccttcaggc tccgatctgc gagctattgg ctatgtggtt ccaggactca tagcgaatga
      421 tatatggaag caagggttct ggaggacact gcttggaagc cttgtaatta ccctagcgac
      481 tcgtgtcgtg ctactcttga ttttggtttg agtccttcca tgaaaaattc gattgcaaaa
      541 caaaggcaaa atcctgctct ttttgtacga gttctaattg cgctcgcttc gctcgcgctc
      601 ggccttgtcg ccacagaatt gctagcgcac aatgaacctc atccggctgc cgaagcgatg
      661 aacaaggcag ctcgacgaat ggcccaagca gaagctgtag tcgcgaaaaa gcgccttgct
      721 atgggattgc ccatggatcc tgctctagac ccatatcaaa cgggcctaat cggtgcagaa
      781 tcgagcattt tgacaactac tgtaggcgat atagtgtcca agaggacgag cacaaatacg
      841 gcttttgcag cccttattgt gcgatatttt catgatattg gtcttgagtc gggagacagg
      901 atagccgttg gctcatcggg ctcgtttccg gcaatcctta tagccgtact ttctgcttgt
      961 gcggagactg gagtggagcc gatcgttatc gcctcaatag gcgcttcgga atatggcgcc
     1021 aatatcgaag gctttagcaa tgccgagata atggcagcat gcagagacgc tggagtgctg
     1081 ccatatatgc ctattgcgat ttctcccggc ggggaggatg atctggggat ttcttcaatg
     1141 tatagacttg agccaagcga cgagctggcg aagtatgcac agcgtgttgc aaaaagtcta
     1201 gatattcctc tcattcaatc tccagatctc gagagctctt ttagggccca tctcgaagta
     1261 tatgaaaagg catctccaat aaaggctttt gtcaacatag ggggagcgga tgtcaatttt
     1321 ggtagcggcc cagcctcgct caagctgaaa ccaggactca tcgtttcatc ggctcgaaca
     1381 aggggaaccg atttgggtaa attgcataag caagtaccaa gagatccaaa aaaatctgcc
     1441 cgacaggaga caagcagcgc agggacaatg gacgctaata tcggcttaga tgcacaagct
     1501 ggtcttattg cctattatat ggccaaaggc cttcctgtcc tacattttct aaatattaaa
     1561 ggccttgcag tgcaaagtgg aatacctgta gatggagatc cgagagctcc tcttccttcc
     1621 agtatcgtca ctatgcgcat aaagccgagc tggcctcttc ttataagtct cttccttgct
     1681 ggtacggcac ttgccatccg aaagcctctt agatactatc catagcaata
//
